MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Comparative Clinical Pathology' source. Thu, 09 Feb 2012 00:42:09 +0100 http://www.medworm.com/index.php?rid=5661205&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8x402884h4503112%2F This study aimed to investigate the effect of piribedil, a drug used for the treatment of Parkinson’s disease and which has direct dopaminergic stimulating action, on the acute hepatic injury in mice. Hepatotoxicity was induced by CCl4 orally (0.28 ml/kg). Piribedil at three dose levels (4.5, 9, or 18 mg/kg) or silymarin (25 mg/kg) was given orally daily for 7 days, starting at time of administration of CCl4. Liver damage was assessed by determining liver serum enzyme activities and by hepatic histopathology. Piribedil administration lessened the increases in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) and also prevented the development of hepatic necrosis caused by CCl4. The effect of piribedil was dose-d... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5661205 Thu, 02 Feb 2012 18:14:50 +0100 5661205 http://www.medworm.com/index.php?rid=5661204&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg0x7217344940664%2F Abstract  Hepatic stellate cells (HSCs) play a critical role in the fibrogenesis of the liver, so they are the target cells of antifibrotic therapy. Activated HSCs, but not quiescent HSCs, express cyclooxygenase-2 (COX-2). The present study was designed to investigate the possible prophylactic and therapeutic effects of a selective COX-2 inhibitor (celecoxib) on liver fibrosis induced by thioacetamide (TAA) in rats. Forty-two male albino rats were divided into five groups: group I, negative control; group II, model of fibrosis; group III, preventive model before induction of fibrosis where celecoxib was given for 4 weeks before TAA; group IV, preventive model at the time of induction of fibrosis where celecoxib was given concomitantly with TAA for 6 weeks; group V... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5661204 Thu, 02 Feb 2012 18:14:50 +0100 5661204 http://www.medworm.com/index.php?rid=5651021&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F84786606gx635223%2F Abstract  The aim of our study was to evaluate the effect of Orchis anatolica roots ethanol extraction on alloxan-induced diabetic rats. Thirty-six albino rats (200 g) were used in this experiment and divided into six groups. Diabetes was induced in five rat groups by a single intraperitoneal injection of alloxan (150 mg/kg body weight). After hyperglycemia was conformed, one rat group was considered as diabetic control and one group was treated with glibenclamide (10 mg/kg body weight/daily) where the remaining three groups received daily treatments with three different doses of O. anatolica extract namely 200, 400, and 800 mg/kg body weight for 10 days. Body weight and fasting blood sugar levels were recorded throughout and by the end of the treatm... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651021 Mon, 30 Jan 2012 06:32:07 +0100 5651021 http://www.medworm.com/index.php?rid=5651022&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F401080jk442h5252%2F Abstract  Oxidative stress is believed to play an important role in the pathogenesis of smoking-induced chronic obstructive pulmonary disease (COPD). We hypothesized that low serum activity level of paraoxonase-1 (PON1) that play a protective role in the lungs by metabolizing lipid peroxides and genetic polymorphism of antioxidant enzymes that detoxify cigarette smoke products such as microsomal epoxide hydroxylase-1 (m-EPHX-1) would be associated with increased susceptibility to COPD in smokers. The study was conducted on patients admitted to Chest Diseases Department, Kasr Al-Aini Hospital, Cairo University. Sixty subjects were divided into three groups—25 COPD patients, 25 chronic smokers without COPD, and 10 healthy nonsmokers. Pulmonary function tests were done for c... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651022 Fri, 27 Jan 2012 06:50:50 +0100 5651022 http://www.medworm.com/index.php?rid=5651023&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy487144023617057%2F In this study, we evaluated the potential use of Nicotina tabacum in controlling leech populations. After treating the leeches with different concentrations of N. tabacum for 30 min, the LC50 values were analysed. Copper sulphate and ammonium chloride were used as positive controls. The anti-parasitic effect of N. tabacum was compared with positive controls. Our data showed that the LD50 values for N. tabacum were 13 × 104 ppm which was considerable compared with positive control. Our finding offers an opportunity for using the plant for anti-parasite purposes. Content Type Journal ArticleCategory Brief CommunicationPages 1-3DOI 10.1007/s00580-012-1413-xAuthors Mahmoud Bahmani, Member of Young Researchers Club, Islamic Azad University of Dehloran Branch, Dehloran, I... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651023 Thu, 26 Jan 2012 16:43:52 +0100 5651023 http://www.medworm.com/index.php?rid=5651024&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp43h27j8n21k70g4%2F In this study, the effect of oral administration of Marze (Satureja khuzestanica) and mountainous Lael (Oliviera decumbens) extracts on haematological parameters and lysozyme activity stimulation in Common carp was investigated during a 5-week period. At the end of this period, blood samples were taken for haematological and immunological assays (lysozyme activity). The result showed that white blood cell count (p > 0.05) and the ratio of white blood cells (p > 0.05) were not significantly affected by plant extract administration. There were significant differences between the serum lysozyme activities in different treatments (p < 0.05). Generally, it can be concluded that Marze extract, as an immune stimulant, has a positive effect on the immune system and increa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651024 Thu, 26 Jan 2012 16:43:51 +0100 5651024 http://www.medworm.com/index.php?rid=5651027&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3k223327836t6366%2F In conclusion, the antioxidant defense of the RBCs has a considerable role to prevent the occurrence of anemia in malignant ovine theileriosis. Content Type Journal ArticleCategory Original ArticlePages 1-7DOI 10.1007/s00580-012-1419-4Authors S. Nazifi, Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, IranS. M. Razavi, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IranE. Rakhshandehroo, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IranR. Sadoughifar, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IranM. Sharifian, Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Journal C... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651027 Thu, 26 Jan 2012 06:44:01 +0100 5651027 http://www.medworm.com/index.php?rid=5651026&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb8103370r20t0336%2F Abstract  The causes of renal cell carcinoma (RCC) were investigated in 115 nephrectomized Japanese. Among them, 110 nephrectomized Japanese had RCC as follows: 86 clear cell RCC, 8 papillary RCC, 8 chromophobe RCC, 7 dialytic–multicystic RCC, and 1 liposarcoma, while 5 nephrectomized Japanese had benign renal tumors of oncocytoma (1), angiomyolipoma (2), and hemangioma (2). In the eight chromophobe RCC, three cases that resulted in death involved medullary RCC showing extensive oncogenic features. Double or triple cancers were found in 17 clear cell RCC, 5 papillary RCC, 1 chromophobe RCC, and 1 dialytic–multicystic RCC. Among 31 non-RCC found in 24 RCC cases, 23(74%) non-RCC were removed prenephrectomy. Prenephrectomy adenocarcinoma and postnephrectomy squamous cell c... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651026 Thu, 26 Jan 2012 06:44:01 +0100 5651026 http://www.medworm.com/index.php?rid=5651025&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk770486331856uh3%2F Abstract  Immune thrombocytopenic purpura (ITP) is an autoimmune syndrome involving platelets destruction and suppression of platelet production that may predispose to bleeding. Protein tyrosine phosphatase non-receptor 22 gene (PTPN22) is an important negative regulator of signal transduction through the T-cell receptors (TCR). A single nucleotide polymorphism (SNP) 1858C > T within this gene was reported to be associated with increased risk of autoimmune diseases. The aim of the work was to study the frequency of the 1858C > T single nucleotide polymorphism in the PTPN22 gene in Egyptian ITP patients. After full clinical and laboratory examination of our subjects, the expression of the PTPN22 (1858C > T) gene polymorphism was analyzed in 60 ITP patient... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651025 Thu, 26 Jan 2012 06:44:01 +0100 5651025 http://www.medworm.com/index.php?rid=5651028&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm2051m75x2h71817%2F Abstract  Sickle cell disease (SCD) is an inherited disorder of sickle hemoglobin affecting millions of people worldwide. The current study aimed at detecting the prevalence of MBL2 exon-1(codons 52, 54, and 57) and promoter region (-221, X/Y) genetic polymorphisms in Egyptian children with SCD to clear out its possible role as a genetic risk factor for susceptibility to vaso-occlusive crisis (VOC) and/or infections. Genotyping of exon-1 and the promoter region was done by polymerase chain reaction for 50 SCD patients and 50 healthy controls. The frequency of MBL2 promoter polymorphism was 32% for the heteromutant genotype, Y/X and 8% for the homomutant genotype, and X/X with no statistical difference in the distribution of the mutant genotypes between SCD patients and cont... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5651028 Wed, 25 Jan 2012 18:13:50 +0100 5651028 http://www.medworm.com/index.php?rid=5639878&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1r5g505177481826%2F In this study, a questionnaire was distributed among 45 residential areas in 22 rural zones of the southern areas of Ilam province. The objective of this study was the recognition of natural medicinal methods using medicinal plants, and the classification of ethnoveterinary applications and collection of domestic science. Twenty-two medicinal plants from 16 families were identified. The main application of these plants was for the detection and treatment of digestive disorders using Citrullus colocynthis, Aristolochia clematis, Scrophularia deserti, Quercus brantii, Ceracus microcarpa, Echium strigosa, Pistacia atlantica, and Pistacia khinjuk which have been applied using Euphurbia graminifolius, Peganum harmala, Salsola rigida, Artemisia herba-alba, Amygdalus arabica, jolbak of salt wa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639878 Wed, 25 Jan 2012 06:52:33 +0100 5639878 http://www.medworm.com/index.php?rid=5639880&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fyn1460486656810h%2F Abstract  One hundred (100) bovine bulk milk samples were tested for the presence of Coxiella burnetii and the detection of associated risk factors. Of the tested samples, 11% tested positive for C. burnetii. There was no correlation between the presence of C. burnetii in bulk milk samples and the kind of dairy herd the samples were taken from the number of cows in each site, and the variety of water and food. The only association that was found corresponded with seasonal samples. Content Type Journal ArticleCategory Original ArticlePages 1-4DOI 10.1007/s00580-012-1406-9Authors Mohammad Kargar, Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, IranAfsaneh Rashidi, Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, IranAbb... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639880 Wed, 25 Jan 2012 06:52:32 +0100 5639880 http://www.medworm.com/index.php?rid=5639879&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F06561178j3620482%2F Abstract  A year-old German shepherd-cross bitch was presented with a history of incontinence and an abnormal posterior gait that worsened on progression. Visual examination did not show ectoparasites. The heart, pulse and respiratory rates as well as rectal temperature were normal. Palpation of the hip joints did not suggest luxation. History also revealed that the bitch was served 2 weeks earlier. The radiographic examination revealed partial fusion of the last lumbar vertebra with the sacrum. The case was handled conservatively with periodic analgesic treatment recommended. A follow-up of the case showed an improved condition, and the bitch whelped four puppies (two dead and two alive) 6 weeks later. It is concluded from this report that the lameness observed w... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639879 Wed, 25 Jan 2012 06:52:32 +0100 5639879 http://www.medworm.com/index.php?rid=5639881&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg41jl852r3q21155%2F Abstract  Following the report of deaths in a few flocks of domestic pigeons in the Mazandaran province, Northern Iran, we conducted an investigation on the live and dead pigeons. We found reddish larvae on the skin of the pigeons and after close analysis of the mouthparts and spiracles; they were identified as second instar larvae of Protocalliphora sp. These blow fly larvae are obligate haematophagous parasites, highly pathogenic and cause high mortality in birds. Mortality in the infected flocks was approximately 25% and mainly among nestlings and young birds. Infected birds were treated with propoxur powder which was very effective, following which no additional mortalities or larvae were found. This is the first report of this myiasis agent in Iran. Content Type Jo... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639881 Tue, 24 Jan 2012 12:25:51 +0100 5639881 http://www.medworm.com/index.php?rid=5639882&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy58109251093725r%2F Abstract  Sarcocystosis caused by different Sarcocystis species and is a zoonotic protozoan infection with worldwide distribution in man and many animal species. In the present study, three techniques — direct inspection, impression smears and digestion method — were used for the diagnosis of Sarcocystis infection in 112 slaughtered sheep in a Kerman abattoir from November 2010 to May 2011. A total of 450 slaughtered sheep were selected randomly during three seasons, and their oesophagus and diaphragm were inspected using naked eye examination. In the second stage, 112 carcasses out of the 450 which had no apparent infection were selected randomly and their meat investigated using two techniques: impression smear with staining and pepsin digestion. The percentage of macro... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639882 Tue, 24 Jan 2012 12:25:50 +0100 5639882 http://www.medworm.com/index.php?rid=5639883&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn777606m40839257%2F We report a giant spiral leiomyoma that presented with dysphasia and weight loss and enucleated successfully. Content Type Journal ArticleCategory Original ArticlePages 1-3DOI 10.1007/s00580-012-1417-6Authors Rahim Mahmodlu, Urmia University of Medical Sciences, Urmia, IranSona Noori-Vahed, Urmia University of Medical Sciences, Urmia, IranPeyman Mikaili, Urmia University of Medical Sciences, Urmia, IranRahim Mehdioghli, Urmia University of Medical Sciences, Urmia, Iran Journal Comparative Clinical PathologyOnline ISSN 1618-565XPrint ISSN 1618-5641 (Source: Comparative Clinical Pathology) Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639883 Tue, 24 Jan 2012 12:25:49 +0100 5639883 http://www.medworm.com/index.php?rid=5639884&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff555416785818814%2F Abstract  H9N2 avian influenza A viruses (AIV) have become panzootic in Eurasia over the last decade and are endemic in Iran since 1998, and inactivated vaccine has been used in chickens to control the disease. The hemagglutinin (HA), one of eight protein-coding genes, plays an important role during the early stage of infection. To study their evolution and zoonotic potential, we conducted an in silico analysis of H9N2 viruses that have infected broiler in Tehran Province, Iran between 1998 and 2007. The complete coding region of HA genes from nine H9N2 subtypes isolated from chicken flocks in Tehran Province during 1998–2007 was amplified and sequenced. Sequence analysis and phylogenetic studies of H9N2 subtype viruses on the basis of data of 9 viruses in this study and ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5639884 Tue, 24 Jan 2012 07:26:50 +0100 5639884 http://www.medworm.com/index.php?rid=5611946&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh8488005r184j847%2F Abstract  The purpose of this study was to compare albumin concentrations in ring-necked pheasants (Phasianus colchicus) using two different dye-binding methods: the bromocresol green (BCG) and bromocresol purple (BCP). High positive correlation was observed for albumin in BCG and BCP methods. Compared to BCP, the BCG-binding method yielded significantly higher (p < 0.0001) albumin values for adult female ring-necked pheasants. Content Type Journal ArticleCategory Original ArticlePages 1-2DOI 10.1007/s00580-011-1396-zAuthors Elizabeth Moreira dos Santos Schmidt, Department of Veterinary Clinics–FMVZ, São Paulo State University, Distrito de Rubião Junior, s/n, 18.618-970 Botucatu, SP, BrazilAntonio Carlos Paulillo, Department of Veterinary Pathology–FCAV, São... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5611946 Tue, 17 Jan 2012 07:17:00 +0100 5611946 http://www.medworm.com/index.php?rid=5611947&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F183480uvxl352304%2F Abstract  A female Holstein calf with a body weight of 35 kg was born alive after a normal pregnancy period in September 2010 at a commercial dairy farm in Karaj, Iran. At birth, the cleft lip, jaw, and palate were obvious. The cleft was approximately 8.4 cm in length and 7.2 cm in width at the widest part. Pulse rate, respiration rate, and body temperature of the calf were 112/min, 42/min, and 38°C, respectively. Respiration was abdominal and irregular. Necropsy revealed that there was no more congenital abnormality except for the facial defect. Radiologic examination revealed that the cleft extended from the median lip to the rostral part of the palate, which made the dorsal nose and the palate separate. Based on examination of the craniofacial skeletal in ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5611947 Tue, 17 Jan 2012 07:16:58 +0100 5611947 http://www.medworm.com/index.php?rid=5611948&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg20051u8581034v5%2F Abstract  A male 2.5-year-old American short hair cat was admitted to the animal hospital with the primary complaints of abdominal distension and depression for several days. Ultrasound revealed a large hypoechoic cavity with a well demarcated thin wall on a liver lobe. On exploratory laparotomy, a hepatic haematoma was observed. Subsequently, hepatic lobectomy was performed. Histologically, an eosinophilic substance stained with Congo red was deposited in the cyst wall which showed an optical property of apple green birefringence under polarized light. Based on immunohistochemical staining, amyloid A was diagnosed. To our knowledge, this is the first report of a spontaneous hepatic haematoma with an amyloid A deposition in a cat. Content Type Journal ArticleCategory Br... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5611948 Tue, 17 Jan 2012 07:16:56 +0100 5611948 http://www.medworm.com/index.php?rid=5611949&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp673156465365588%2F Abstract  The increasing data provides enough evidences confirming the involvement of free radicals and other reactive oxygen species (ROS) superoxide radical ( . O2−), nitric oxide (NO . ), hydrogen peroxide (H2O2) and hydroxyl radicals ( . OH) in a number of physiological and pathological processes. Imbalance between levels of ROS resulting in the body and the capacity of antioxidant defense mechanisms occur oxidative stress (OS). OS is related to a number of structural and functional damages to cells and is involved in the pathogenesis of many diseases, including neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease (PD), amyotrophic lateral sclerosis, and Huntington disease. Defects in oxidative phosphorylation and oxidative damage play ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5611949 Tue, 17 Jan 2012 07:16:55 +0100 5611949 http://www.medworm.com/index.php?rid=5585664&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F076h811110x71283%2F This study aimed to assess plasma concentrations of copper, iron, and zinc during the course of acute Trypanosoma evansi experimental infection in rats. Fifty male Wistar rats were randomly distributed into seven groups: three trypanosome-infected groups (T2, T4, and T6; n = 10 animals per group) and four uninfected controls (C0, C2, C4, and C6; n = 5 animals per group). Animals from trypanosome-infected groups were intraperitoneally injected with 106 trypanosomes/animal. Blood samples were collected by cardiac puncture before infection (day 0; group C0) or on the second (C2 and T2), fourth (C4 and T4), and sixth (C6 and T6) day postinfection. Parasitemia and hematological evaluation were performed to assess the progression of the disease in animals. The difference between group... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5585664 Mon, 09 Jan 2012 19:36:58 +0100 5585664 http://www.medworm.com/index.php?rid=5585666&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6755p28t70332648%2F Abstract  Narasin poisoning was reported in 15 camels, 7 adults and 8 young, after accidental access to poultry feed medicated with 60 g narasin per ton. Fourteen camels died between 3 and 20 days, and one young animal survived the dose after developing a chronic course of a disease. The main clinical signs of narasin toxicity in the dromedary include: weakness of hind limbs, lack of coordination, oedema of dependent parts, inappetence, ruminal atony, myoglobinuria, profound depression, tachycardia, sternal recumbency and death. The lesions were mainly in the heart and skeletal muscles and consisted of multifocal degeneration and necrosis of heart and skeletal muscle fibres with areas of regeneration and lung oedema. There was high enzyme activity for creatine kin... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5585666 Mon, 09 Jan 2012 06:32:48 +0100 5585666 http://www.medworm.com/index.php?rid=5585665&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fep6463kv72833h26%2F Abstract   Trichomonas vaginalis is the cause of one of the most common types of vaginitis, trichomoniasis. Clinical manifestations of symptomatic women are generally nonspecific, but include vaginal discharge, vaginitis and irritation. T. vaginalis infection has also been linked to the increased risk of human immunodeficiency virus transmission. The aim of the study was to compare a real-time polymerase chain reaction (PCR) assay with culture and wet-mount examination for the detection of T. vaginalis. This is a descriptive analytical study. Vaginal swabs from 504 female patients with suspected vaginitis were included in the study. They were subjected to culture, wet-mount microscopic examination (WM), and real-time PCR. Real-time fluorescence resonance energy transfer hybr... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5585665 Mon, 09 Jan 2012 06:32:48 +0100 5585665 http://www.medworm.com/index.php?rid=5573438&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6703n428407x8735%2F Abstract  Six adult dogs were used in an experimental model of osteoarthritis (OA) reproduced by intra-articular injection of sodium monoiodoacetate (MIA) in the left stifle joints. Contralateral joints served as control. Before the first MIA administration and at days 30, 60 and 105, synovial fluid samples were obtained aseptically from both joints to determine some physical (volume, colour, transparency, viscosity) and biochemical parameters (total protein, glucose, lactate dehydrogenase). The results showed that the used chemical model of OA reproduced successfully the dynamics of the disease in dogs. The changes in studied biochemical parameters (substantially decreased viscosity and glucose content and statistically significantly higher total protein content and l... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5573438 Thu, 05 Jan 2012 06:53:11 +0100 5573438 http://www.medworm.com/index.php?rid=5573437&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx2717mu160880126%2F Abstract  The existence of daily rhythmicity of serum total proteins, their fractions and some biochemical parameters in healthy cows (group A) and in cows affected by Brucella abortus (group B) was assessed in order to clarify the role of circadian timing processes in healthy and disease in cows. On each subject, blood samples were collected, every 4 h over a 24-h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, via intravenous cannulas inserted into the jugular vein. On all samples, serum total proteins and their fractions (α1-, α2-, β1-, β2- and γ-globulins), gammaglutamyl transferase (GGT), alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine and urea were assessed. The application of one-way analy... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5573437 Thu, 05 Jan 2012 06:53:11 +0100 5573437 http://www.medworm.com/index.php?rid=5573439&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc267l46848188259%2F Abstract  To evaluate the prevalence, histopathological, and some epidemiological aspects of abomasal ulcers in water buffalo (Bubalus bubalis), the abomasums of 100 randomly selected water buffaloes were examined after slaughter. Type 1 abomasal ulcers were found in 56 buffaloes. There was no significant difference between either sex (P = 0.085) and between the different age groups in the prevalence of the abomasal ulcers. The occurrence of abomasal ulcers had a seasonal pattern, and the majority of lesions were found in the pyloric region of the abomasums. Histopathological examination showed focal to multifocal demarcated areas of necrosis associated with infiltration of inflammatory cells in the mucosa, both acute and chronic inflammatory reactions, a reduced number o... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5573439 Wed, 04 Jan 2012 07:01:35 +0100 5573439 http://www.medworm.com/index.php?rid=5573440&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F460k2mk82577016g%2F This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Sixteen adult female rats were allocated in two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural matting. Ninety days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological parameters were determined using histological techniques. Results revealed a significant increase in body weight and blood glucose in the offspring of diabetic mothers (ODM) compared to that of controls. The weight, volume and diameter of the ovary and ovarian capsule thickness were decreased in the ODM group. The number and diameter of primary, preantral, antral and... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5573440 Wed, 04 Jan 2012 07:01:34 +0100 5573440 http://www.medworm.com/index.php?rid=5573441&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk161832l77677l75%2F Abstract  Individuals with reduced glomerular filtration rate (GFR) are at greater risk for cardiovascular disease and other comorbidities. Creatinine-based equations are used to estimate GFR, identify patients with potential kidney disease, and classify them into different stages since serum creatinine is insensitive to changes in the GFR. The aim of our work was to evaluate diagnostic performance of serum cystatin C and neutrophil gelatinase-associated lipocalin (NGAL) as markers of kidney function in patients with reduced GFR. Fifty cases at different stages of renal impairment and 30 healthy control subjects were tested. Only serum NGAL and cystatin C were higher in stage 2 chronic kidney disease (CKD) when compared to the control group (p < 0.05). For stages 3–... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5573441 Wed, 04 Jan 2012 07:01:33 +0100 5573441 http://www.medworm.com/index.php?rid=5562664&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fgq2513t144231645%2F Abstract  Regaining adequate bone strength, in bone loss, is one of the main purposes for new bone regeneration in bone tissue engineering. Biomechanical hardness test can be one approach to assay bone consistency in new bone formation. In addition, following up the serum alkaline phosphatase (ALP) alterations may help us in order to evaluate bone formation activity. In the current research, two groups of five male white New Zealand rabbits were studied. Two defects, 8 mm in diameter each, were made in each rabbit calvarium, one defect was filled with either human pericardial collagen (HPM) or demineralized bone matrix (DBM), and the other one was left empty as control. Every 10 days post-surgery, the serum ALP level was assessed, for 60 days. After performin... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5562664 Mon, 02 Jan 2012 06:58:20 +0100 5562664 http://www.medworm.com/index.php?rid=5562663&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq8r31452064h8k72%2F Abstract  The comparative effect of raw red chili and red chili treated by gamma irradiation or microwave pasteurization with or without 5-fluorouracil on experimentally induced colon cancer in rats was investigated. Dimethylhydrazine was used to induce colon cancer which is histologically similar to and comparable with human neoplasm. Histopathological examination of dimethylhydrazine-treated rats demonstrated epithelial and goblet cell hyperplasia as well as preneoplastic changes of the rat colon expressed by loss of polarity of the nuclei and abnormal mitosis. The preneoplastic changes were developed to adenocarcinoma in 80% of cases. The liver showed different pathological pictures that included dysplastic changes, preneoplastic foci, and oval cell proliferation. We obs... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5562663 Mon, 02 Jan 2012 06:58:20 +0100 5562663 http://www.medworm.com/index.php?rid=5553718&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fegu562818t160nh2%2F Abstract  The acute phase response is a nonspecific inflammatory reaction of the host that occurs shortly after any tissue injury. The response includes changes in the concentration of plasma proteins called acute phase proteins (APPs). Calf diarrhea is an important disease that occurs in association with the interaction of various infectious agents and calf susceptibility. The economic losses is associated with death loss and treatment costs, reduction of live weight gain, and reduction of productive life span, which may be considerable. The aim of the present study was to identify relationships among APPs in calves with diarrhea in the different clinical features. Holstein calves (50) within 1 day to 4 months old with signs of diarrhea and healthy calves (40) wi... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5553718 Wed, 28 Dec 2011 06:39:59 +0100 5553718 http://www.medworm.com/index.php?rid=5553719&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Feg37138850200554%2F Abstract  Deep vein thrombosis (DVT) is a common multi-factorial disease, with serious short- and long-term complications, and a potential fatal outcome. Many genes are involved in determining the interindividual variation in traits that define the onset and progression of disease, as well as the response to treatment. Several association studies have designed the relationship between factor XII C46T polymorphism and the risk of arterial and venous thrombosis. Some studies reported that FXII gene polymorphism is not associated with venous thrombosis, whereas other studies found an increased risk of venous thrombosis in carriers of a FXII-T variant. We constructed an age–gender–ethnic–matched case–control study including 52 DVT patients and 100 healthy volunteers. C4... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5553719 Tue, 27 Dec 2011 06:33:58 +0100 5553719 http://www.medworm.com/index.php?rid=5553720&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw4w3604x5n3h3773%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00580-011-1384-3Authors Mohammad Moazeni, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731 IranAshkan Roozitalab, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731 Iran Journal Comparative Clinical PathologyOnline ISSN 1618-565XPrint ISSN 1618-5641 (Source: Comparative Clinical Pathology) Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5553720 Tue, 27 Dec 2011 06:33:57 +0100 5553720 http://www.medworm.com/index.php?rid=5553721&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fdl1j1010n8u252ug%2F In conclusion, this study reveals that serum amyloid A, haptoglobin, and fibrinogen are potentially useful and sensitive markers for early determination of bronchopneumonia and can be used as inflammatory indicators of health in calf herds, thereby facilitating treatment decisions. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00580-011-1377-2Authors Ezzatollah Fathi, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Imam St., Azadi Ave, P.O. Box 5166616471, Tabriz, IranRaheleh Farahzadi, Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Chamran Ave, Tehran, IranMohammad Imani, Novel Drug Delivery Systems Department, Iran Polymer and Petrochemical Institute, P.O. Box 14965/... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5553721 Tue, 27 Dec 2011 06:33:56 +0100 5553721 http://www.medworm.com/index.php?rid=5546078&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr52p340877173867%2F Abstract  A feeding trial was conducted to investigate the effects of dietary supplementations of prebiotic, probiotic, and antibiotic to wet wheat-based diets on broiler performance, carcass yield, organ weights, and histomorphological measurements of the small intestine. One hundred and sixty-day-old male broiler chicks were randomly assigned to one of four dietary treatments for 6 weeks. The dietary treatments were: (1) control (1.2 g water/gram of dry feed); (2) control diet supplemented with antibiotic (15 mg/kg of virginiamycin); (3) control diet supplemented with 1 g/kg probiotic (8 × 105 colony forming units (CFU) of Bacillus subtilis/gram); and (4) control diet supplemented with 1 g/kg prebiotic (Aspergillus sp. meal). The body weight an... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546078 Fri, 23 Dec 2011 16:52:41 +0100 5546078 http://www.medworm.com/index.php?rid=5546077&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F283807231j0h6k15%2F This study investigated the prevalence of and clinicopathologic findings associated with grade 3 ascites in dogs presented at three major veterinary clinics in Enugu State, Nigeria between January and December 2010. Blood samples were collected from reported cases for haematology and serum chemistry tests, while the ascites fluid was collected for cytological and relevant clinical chemistry determinations. Blood samples were also collected from 12 apparently healthy non-ascitic dogs from the study population to serve as control. All haematology, cytology and clinical chemistry tests were carried out following standard procedures. Results showed that out of a total of 1,788 dogs presented in the three clinics during the study period, 14 had grade 3 ascites (0.78%), and there were no s... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546077 Fri, 23 Dec 2011 16:52:41 +0100 5546077 http://www.medworm.com/index.php?rid=5546079&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7630362p63878021%2F Abstract  The study was carried out from September 2010 to September 2011 at Kerman Municipal abattoir to assess the status of hydatidosis in cattle. Out of the total 1,000 cattle examined visually and manually (palpation and incision), 86 (8.6%) were found harboring hydatid cysts. A significantly higher infection was detected in older cattle (P < 0.05) than younger ones. Of the total of 86 infected, 13 (15.11%) had hydatid cysts only in the lung, 33 (38.37%) in the liver, 2 (2.32%) in the muscles, while the rest 38 (44.18%) had multiple organ infections. Of the 125 viscera harboring hydatid cysts, the highest (56.8%) was the liver followed by the lungs (40.8%) and the muscles (2.4%). From the total of 721 hydatid cysts counted, 205 (28.43%), 222 (30.79%), 45 (6.24%),... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546079 Fri, 23 Dec 2011 06:47:43 +0100 5546079 http://www.medworm.com/index.php?rid=5546081&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw607545m6j004115%2F Abstract  The effect of Cannabis sativa extract on acute liver injury caused by acetaminophen or carbon tetrachloride (CCl4) was studied in rats. Cannabis sativa was given at doses of 5 or 10 mg/kg (expressed as Δ9-tetrahydrocannabinol) once daily intraperitoneally (i.p.) for 2 days and simultaneously with acetaminophen or CCl4. Rats were killed 24 h after acetaminophen or CCl4 administration. Reduced glutathione (GSH), lipid peroxidation (malondialdehyde; MDA) and nitric oxide (nitrite/nitrate) concentrations were measured in the liver. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were determined in serum. Hepatic injury was also determined via histological examination of liver sections. The administration of o... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546081 Fri, 23 Dec 2011 06:47:42 +0100 5546081 http://www.medworm.com/index.php?rid=5546080&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn361081833454488%2F Abstract  The efficacy of Kelamidium® in the prevention and treatment of experimental Trypanosoma brucei brucei infection of albino rats was studied. Adult albino rats (55) weighing between 147 and 240 g were used for the study. The rats were kept in metal cages in a fly-proof house and were adequately fed and given water ad libitum. Two experiments were carried out. In experiment I (chemotherapy), 30 adult albino rats were divided into six groups of five rats each, whereas in experiment II (chemoprophylaxis), 25 adult albino rats were divided into five groups of five rats each. In both experiments, groups I and II were uninfected control and infected untreated control, respectively. In experiment I, rats in groups III and V were each infected with 5.0 × 105 trypa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546080 Fri, 23 Dec 2011 06:47:42 +0100 5546080 http://www.medworm.com/index.php?rid=5546082&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fph03655526033226%2F Abstract  Toxic compounds such as disulfides of onions cause oxidative-induced haemolysis in several animal species. In order to study the outcomes of long-term onion consumption on some oxidative haemolysis markers, 12 adult female goats were allocated to three groups, receiving 0% (served as control), 30% (dry matter basis) and 60% spring-grown onion for 60 days. Blood samples were obtained before feeding the onion and every 10 days up to 80 days for measuring malonyldialdehyde (MDA), methaemoglobin (MetHb), mean corpuscular fragility (MCF), serum-free haemoglobin and serum lactate dehydrogenase (LDH) and for evaluating their relation to packed cell volume (PCV) and haemoglobin (Hb) concentrations. PCV and Hb concentrations reduced, however, remained within... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546082 Wed, 21 Dec 2011 20:12:41 +0100 5546082 http://www.medworm.com/index.php?rid=5546083&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxj120nj7q816v420%2F Abstract  The aim of this study was to determine if physiological changes occur in plasma total proteins (TPP) and albumin (ALB) concentrations and the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) in Spanish Purebred mares during pregnancy. Blood samples were collected by jugular puncture and anticoagulated in tubes with lithium heparin. In plasma obtained after centrifugation the concentrations of TPP and ALB and activities of AST, ALT, LDH, CK, ALP and GGT were determined by spectrophotometry. Pregnancy caused an increase in TPP and decrease in AST, CK and GGT (p < 0.05) without modifications in ALB, ALT, LDH and ALP c... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546083 Wed, 21 Dec 2011 20:12:40 +0100 5546083 http://www.medworm.com/index.php?rid=5546084&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F61m707041788l22j%2F This study aimed to test 14-day oral administration of garlic (100 mg/kg) for its effects in alloxan-induced diabetic dogs in comparison with metformin (1,700 mg/dog/day) anti-diabetic activity. The mean decrease in blood glucose was 9.27 mg/dl for metformin and 25.93 mg/dl for garlic. The results showed that treatment with metformin non-significantly reduced blood glucose level (p > 0.05) and significantly increased serum insulin level in diabetic dogs (p < 0.05). In contrast to metformin, garlic resulted in a significant decrease in the level of blood glucose, with a concomitant significant increase in the serum insulin level in diabetic dogs (p < 0.05). Garlic had a mild but significant blood glucose-lowering effect and the long-term use of t... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5546084 Wed, 21 Dec 2011 20:12:39 +0100 5546084 http://www.medworm.com/index.php?rid=5516641&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh678367720228667%2F Abstract  An experimental study examined the effect of sub-conjunctival platelet-rich plasma (sPRP) in combination with topical acetylcysteine on corneal alkali burn ulcers in rabbits. A total of 20 rabbits were used in this study. After collecting intracardiac blood samples from ten rabbits, platelet-rich plasma was obtained by centrifugation. Alkali wounds were inflicted on the central corneas of rabbits by applying a round filter paper, 6.0 mm in diameter, soaked in 1 M NaOH for 60 s. Only one eye in each rabbit was used. A total of 20 rabbits were allocated into four groups of five animals each. Group 1 served as the control group. Group 2 received 3% N-acetylcysteine (NAC) topically three times daily for 2 weeks. The third group received only sPRP wh... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5516641 Thu, 15 Dec 2011 16:49:41 +0100 5516641 http://www.medworm.com/index.php?rid=5516642&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8312575717684224%2F Abstract  A 5-year-old female cat was presented at Kamphaengsaen Animal Hospital for a complication of jaundice. Haematology revealed mild non-regenerative anaemia, lymphopenia, thrombocytopenia, hyper-proteinaemia and icteric plasma. The inclusion bodies in the platelets were detected in a stained blood smear. Partial sequences of the 16S rRNA gene from the isolate indicated that it had the closest relation to Anaplasma platys sequences from many dogs in Thailand and those obtained from GenBank. This is the first report of Anaplasma platys in a naturally infected domestic cat from Thailand. Content Type Journal ArticleCategory Brief CommunicationPages 1-4DOI 10.1007/s00580-011-1378-1Authors Chaleow Salakij, Department of Pathology, Faculty of Veterinary Medicine, Kaset... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5516642 Thu, 15 Dec 2011 16:49:38 +0100 5516642 http://www.medworm.com/index.php?rid=5516643&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F733804h07q03750w%2F In conclusion, in the present study, it was shown that DA causes alterations in the clinical, hematological, and biochemical profiles and these alterations can be more severe when DA is concurrent with other diseases. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00580-011-1382-5Authors M. Mokhber Dezfouli, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, IranZ. Eftekhari, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, IranS. Sadeghian, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, IranA. Bahounar, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, IranM. Jeloudari, Urmia Universit... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5516643 Thu, 15 Dec 2011 06:43:14 +0100 5516643 http://www.medworm.com/index.php?rid=5516644&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr2818254n2184028%2F This study was carried out to determine the effects of methanolic extracts of Allium sativum L. on Limnatis nilotica compared with Niclosomide. In this experimental study in September 2010, a number of leeches (70 in total) from the southern area of Ilam province were prepared, and the effects of methanolic extract of A. sativum L. with Niclosomide as the control drug were compared and distilled water was evaluated as the placebo group which investigated L. nilotica using anti-leech assay. The average time of paralysis and death of L. nilotica for Niclosomide (1,250 mg/kg) and the methanol extract of A. sativum L. (600 μg/ml) were 6.22 ± 2.94 and 68.44 ± 28.39 min, respectively. Distilled water and garlic tablets at a dose of 400 mg were determined as the ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5516644 Wed, 14 Dec 2011 16:40:36 +0100 5516644 http://www.medworm.com/index.php?rid=5507265&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj2351065481x671j%2F Abstract  Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. The aim of the present study was to investigate the presence of classical enterotoxins of S. aureus in raw milk in Iran. For this purpose, from January 2011 to October 2011, a total of 88 raw milk samples from sheep (n = 28), goat (n = 27), camel (n = 14), and water buffalo (n = 19) were collected from 64 randomly selected herds in Isfahan, Chaharmahal va Bakhtiari, and Khuzestan provinces, Iran, and tested for staphylococcal enterotoxins (SEs) presence by enzyme-linked immunosorbent assay method. Out of 88 samples studied, 11 samples (12.5%)... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5507265 Mon, 12 Dec 2011 06:33:09 +0100 5507265 http://www.medworm.com/index.php?rid=5507267&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhp6j37601558m96v%2F Abstract  The genetic abnormalities that contribute to the etiology and prognosis of multiple myeloma (MM) are not fully understood. Polymorphisms of various genes encoding drug transporters and drug targets are potential factors that can influence the risk of developing MM as well as outcome of chemotherapy. NR3C1 gene encodes glucocorticoid receptors which are the cellular target of dexamethasone, the most active component of the VAD scheme used in MM patient treatment. The aim of this study is to look into the influence of investigated polymorphisms on the predisposition to MM development and the efficacy of glucocorticoid-including therapy. The frequencies of functional polymorphisms N363S and BclI of NR3C1 gene were evaluated using PCR-RFLP in 96 MM patients and 55 cont... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5507267 Mon, 12 Dec 2011 06:33:08 +0100 5507267 http://www.medworm.com/index.php?rid=5507266&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr4w5584877151526%2F In this study, we examined the hypothesis of whether genetic polymorphisms in glutathione S-transferase M1 (GSTM1) and cytochrome P450 1A1 (CYP1A1) are related to the risk of developing CRC in Egyptian patients. The association of polymorphisms of CYP1A1 and GSTM1 with CRC was investigated in 40 patients (group I) and 40 controls (group II) using multiplex PCR for GSTM1 and PCR-RFLP for CYP1A1 polymorphism detection. The GSTM1 null genotype was more frequent in group I (45%) than in group II (35%), but this difference was not statistically significant [odds ratio (OR), 0.658 and the 95% confidence interval (CI), 0.217–1.997]. The relationship of GSTM1 deficiency and CRC risk was performed between both sexes; p = 0.213, tumor site p = 0.232, and pathological type p = 0.2... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5507266 Mon, 12 Dec 2011 06:33:08 +0100 5507266 http://www.medworm.com/index.php?rid=5488949&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F80176102035x3202%2F Abstract  To investigate the role of sex steroids of the seminal fluid on sperm quality, the relationships between sex steroids and milt quality parameters (sperm motility and sperm production) were investigated in the Persian sturgeon. The seminal fluid levels of 17α,20β,21-trihydroxy-4-pregnen-3-one (20βs), and 11-ketotestosterone (11-KT) had positive relationships with sperm motility characteristics (percentage and duration of motility) and sperm density. Also, no relationships were found between other sex steroids including: Testosterone (T), progesterone (P), 17α-hydroxyprogesterone (OHP), and milt quality parameters. The good correlation of 20βs and 11-KT of the seminal fluid with sperm motility and sperm density suggests that these steroids may be important horm... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5488949 Tue, 06 Dec 2011 17:01:02 +0100 5488949 http://www.medworm.com/index.php?rid=5488950&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw24218841442746r%2F Abstract  A study was conducted to evaluate the effect of Salmonella Gallinarum (9, 12) infection on broiler chicks infected through oral and intraperitoneal routes. One hundred and ten broiler chicks (1 week of age) were divided into three groups: group CR (30 chicks) were kept uninfected and served as control group, group OR (40 chicks) were inoculated orally with Salmonella Gallinarum (109 organisms/ml) and group IP (40 chicks) were infected intraperitoneally with Salmonella Gallinarum (109 organisms/ml). Three birds from each group (including those that die) were sacrificed at 3, 5, 7, 14, 21, 28, 35 and 42 days post infection (DPI). Clinical signs were noticed as early as 12 h after intraperitoneal infection and on 3 DPI after oral infection... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5488950 Tue, 06 Dec 2011 07:01:34 +0100 5488950 http://www.medworm.com/index.php?rid=5488951&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv766p8l24j763251%2F The objective of this study was to determine the Salmonella prevalence, the serotypes involved, and antimicrobial susceptibility patterns of Salmonella isolates recovered from fish, shrimp, lobster, and crab in Iran. A total of 384 samples of fish, shrimp, lobster, and crab were collected in three provinces along the Persian Gulf in the south coast of Iran. Samples were collected at the end of each month from September 2009 to May 2011. All samples were evaluated for the presence of Salmonella, stereotyped and tested for antimicrobial susceptibility. There was an overall Salmonella prevalence of 5%. Salmonella was isolated from a significantly larger number of fish (10.4%) than shrimp (1.8%; P ≤ 0.05). No Salmonella was isolated from lobster and crab samples. Salmonella isolates re... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5488951 Tue, 06 Dec 2011 07:01:32 +0100 5488951 http://www.medworm.com/index.php?rid=5478658&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb437305500661476%2F Abstract  The goal of this study was to evaluate the effect of unilateral ureteral ligation on blood constituents, renal histopathology and ultrasonography in dogs. A total number of 25 apparently healthy adult mongrel dogs found Assiut City, Egypt, were subjected to study. Animals were divided into five equal groups: the left ureter was ligated for 2, 7, 14 and 21 days in groups, I, II, III and IV, respectively, while group V represented the control group. The animals in groups I, II, III and IV were euthanized on days 2, 7, 14 and 21, respectively. Right and left uretronephrectomies were performed and specimens were taken for the histopathological examination. Results revealed significant increases in total leucocytes and neutrophils counts from day 2 to day 17. Ther... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5478658 Fri, 02 Dec 2011 06:45:38 +0100 5478658 http://www.medworm.com/index.php?rid=5478659&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F26xh86350p41p73m%2F Abstract  We studied the toxic effects of the methanol extract from the stem bark of Diospyros canaliculata via oral administration in rats. Extract was tested in an oral 28-day study at doses of 0.5, 1 and 2 g/kg body weight (bw). Toxicological endpoints examined included blood cell counts and selected organ weights, histopathological examination of the liver and kidneys tissue and biochemical parameters including alkaline phosphatase (ALP), aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT). The extract significantly increased concentrations of serum ALP, AST, total protein and urea at 2 g/kg bw. A significant increase (p < 0.05) was also noted in ASAT activity in 20% liver homogenate of rats receiving the highest dose. Results suggest th... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5478659 Thu, 01 Dec 2011 06:57:48 +0100 5478659 http://www.medworm.com/index.php?rid=5478660&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F118267m831706843%2F This study aimed to evaluate the capability of chelating therapy in reducing the immunotoxic and hematotoxic effects induced by aluminum chloride (AlCl3). For this purpose, 40 male, adult, New Zealand white rabbits were divided into four groups with 10 animals each [control, AlCl3, and hydroxyethyl-ethylene diaminetriacetic acid (HEDTA) or Tiron plus AlCl3 groups]. Aluminum chloride administered via drinking water in a dose of 20 mg/l for 3 months. After that HEDTA or Tiron was administered i.p. at a dose of 50 and 471 mg/kg b.w., respectively, for 21 days, three times/week. Aluminum chloride-exposed rabbits showed a significant decrease in the number of red blood cells, blood hemoglobin concentration, and hematocrit value. Blood δ-aminolevulinic acid dehydratase ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5478660 Thu, 01 Dec 2011 06:57:46 +0100 5478660 http://www.medworm.com/index.php?rid=5470565&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmv69x57375207mv3%2F This study was designed to assay the SLP of albino rats of varied ages and sexes. A total of 480 Sprague Dawley albino rats were used for the study, composed of 240 females and 240 males. The age sets studied were rats of 4, 6, 8, 10, 12, 16, 20, 30, 40, and 50 weeks of age. For each age set, the SLP of 24 females and 24 males was assayed. Standard biochemical procedures were followed in the SLP assay after a 12-h overnight fast of the rats. Results showed that serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC) significantly rose (p < 0.05) from their week 4 values in both sexes to a peak recorded at week 6 of age before a progressive decrease across the ages, while the serum triglyc... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5470565 Tue, 29 Nov 2011 22:20:02 +0100 5470565 http://www.medworm.com/index.php?rid=5424264&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F71512g18l9621266%2F Abstract  Infections with bacteria of the genus Salmonella are responsible for a variety of acute and chronic diseases in poultry. Infected poultry flocks are also among the most important reservoirs of salmonellae that can be transmitted through the food chain to humans. Citrobacter belongs to the Enterobacteriaceae family which is closely related to Salmonella. The aim of this study was to examine goose eggs contaminated with Citrobacter spp. and Salmonella spp. and determine the drug resistance pattern of the isolated organisms. Two hundred and forty goose eggs were collected in Zabol region and were transferred to the microbiology laboratory of Zabol University. The egg shells were thoroughly disinfected and the interior contents of individual eggs were pooled into a steri... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5424264 Wed, 16 Nov 2011 06:47:37 +0100 5424264 http://www.medworm.com/index.php?rid=5424263&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff03pg55p21u02j73%2F Abstract   Astragalus verus Olivier., Fabaceae, has been used as an immunopromoting remedy in Kurdish ethnomedicine. Here, phagocytic (nitroblue tetrazolium (NBT) dye reduction and lysosomal enzyme activity of mouse splenocyte), (co)mitogenic (mouse splenocyte reduction of methyl thiazolyl tetrazolium (MTT)), and antioxidative (nitrite-induced methemoglobin formation) activities of A. verus extracts and chemical compositions of their essential oils (gas chromatography–mass spectrometry) were investigated. All aqueous (AQ), acetone (AC), and methanol (ME) extracts of aerial parts and root polysaccharide extract (PS) enhanced phagocytic activity compared with the control. The phagocytic activities assayed by lysosomal enzyme activity were in agreement with the values obtain... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5424263 Wed, 16 Nov 2011 06:47:37 +0100 5424263 http://www.medworm.com/index.php?rid=5424266&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw4121660p0k2678h%2F Abstract  The use of mesenchymal stem cells (MSCs)-derived equine umbilical cord blood in cell grafts transplantation is advantageous; therefore, preservation of these cells is of utmost importance in repair therapies. To evaluate the viability ratio of the MSCs obtained from equine umbilical cord blood after cryopreservation, umbilical cord blood obtained from nine 46–48-week-old foals were purified for harvesting MCs. The purified cells were frozen from the first to tenth passages and stored in liquid nitrogen. After thawing, the cell viability was assessed through trypan blue staining procedure. The highest viability (>80%) ratio was observed with the cells derived from the first passage in 1 and 8 weeks after cryopreservation. However, the viability of cells w... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5424266 Wed, 16 Nov 2011 06:47:35 +0100 5424266 http://www.medworm.com/index.php?rid=5424265&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe826633gr772101n%2F This study was carried out to investigate the fauna of hard ticks in domestic animals in Sari suburb. Ticks were collected from 2,356 animals including cattle, goat, and sheep and then preserved in 70% alcohol. They were removed through different grades of ethanol, after dehydration, and fixed with Canada balsam and were examined under binocular for identification of ticks based on diagnostic keys. A total of 2,356 animals (cattle, goat, and sheep) were examined from September 2009 to August 2010 randomly to determine the prevalence of ticks. Among these animals, maximum infection was found in goats (46.16%) followed by cattle (32.49%) and sheep (21.35%). Totally, six species were identified. The most frequent species were Hyalomma marginatus (41.20%) followed by Hyalomma anatolicum ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5424265 Wed, 16 Nov 2011 06:47:35 +0100 5424265 http://www.medworm.com/index.php?rid=5424267&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw7802t548776p031%2F Abstract  During pregnancy, metabolic adjustments occur in the mare to preserve iron stores in order to have adequate amounts for the growth and development of the equine fetus. The aim of this study was to analyze the evolution of hematocrit, iron, and ferritin in Spanish Purebred mares during pregnancy. A total of 31 Spanish broodmares aged 4–17 years old were studied during three similar periods of pregnancy (I, II, and III). Hematocrit (packed cell volume, PCV) was determined by microhematocrit and iron and ferritin concentrations were analyzed using spectrophotometry and turbidimetric methods, respectively. Pregnancy in the Spanish mare was characterized by a progressive increase in PCV and serum iron and ferritin concentrations. The physiological state of pregn... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5424267 Tue, 15 Nov 2011 06:49:08 +0100 5424267 http://www.medworm.com/index.php?rid=5411550&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fug64q1l65525w34g%2F This study was based on the hypothesis that a single-nucleotide polymorphism in the promoter region of the interleukin-6 (IL-6) gene influences its transcription and thus affects serum levels of IL-6 and hence of the high-sensitivity C-reactive protein (hs-CRP). This may lead to insulin resistance and consequently type 2 diabetes. This study was performed on 60 subjects: 30 T2DM patients and 30 non-diabetic participants. For all subjects, the serum level of IL-6 was assayed by enzyme chemiluminescence, serum hs-CRP was assayed using a particle-enhanced immuno-turbidimetric assay and the IL-6 –174G/C gene polymorphism was detected by PCR-RFLP. There was no statistically significant association between the IL-6 –174G/C gene polymorphism and the presence of diabetes mellitus or with... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411550 Mon, 14 Nov 2011 16:52:39 +0100 5411550 http://www.medworm.com/index.php?rid=5411551&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff12856gpp0124174%2F Abstract  The aim of the present study was to determine the physiologic electrophoretic pattern of serum proteins in clinically healthy calves and cows using agarose gel electrophoresis, and to describe the possible influence of age on the concentrations of serum protein fractions in young and adult cattle. Into the evaluation we included 14 clinically healthy calves of a Slovak spotted breed and its crossbreeds at the age of 4–6 months, and 13 clinically healthy cows of the same breed at the age of 3–5 years. Blood serum was analyzed for total serum protein concentrations, and for the absolute and relative values of serum protein fractions using agarose gel electrophoresis. In cows we found significantly higher total serum protein concentrations than in calve... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411551 Mon, 14 Nov 2011 06:35:24 +0100 5411551 http://www.medworm.com/index.php?rid=5411552&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx50n4g080lt63765%2F Abstract  The effect of different concentrations of clove solution on blood biochemistry of common carp Cyprinus carpio was investigated. Fish were exposed to 700, 1,000, 2,000, 3,000, 4,000, and 5,000 ppm clove solution over a period of 300, 200, 150, 100, 75, and 70 s, respectively, to reach stage 4 anesthesia. Immediately after induction, blood samples were collected to determine biochemical characteristics. Results showed significant (P < 0.0001) changes in serum cortisol and glucose levels; however, no significant changes were found in serum levels of triglyceride, total protein, cholesterol, albumin, calcium, chloride, or sodium. Serum levels of cortisol and glucose were similar (P > 0.05) in fish exposed to concentrations 4,000 and 5,000 ppm... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411552 Mon, 14 Nov 2011 06:35:23 +0100 5411552 http://www.medworm.com/index.php?rid=5411553&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl3x66x0148w56kk8%2F Abstract  An 8-month female shitzu was presented with depression, anorexia, fever, weakness, lethargy, consecutive coughing, sneezing and severe itching of ears. There was otitis externa with corneal ulcer and keratoconjunctivitis. Microscopic examination of impression smears of all samples revealed numerous yeasts typical of Malassezia sp. Polymerase chain reaction (PCR) and additional tests confirmed the identification of Malassezia pachydermatis. Histopathological examination of skin biopsies revealed oval spores in the superficial layer of the affected skin. There were also follicles containing Demodex spp. Content Type Journal ArticleCategory Brief CommunicationPages 1-4DOI 10.1007/s00580-011-1344-yAuthors Samaneh Eidi, Department of Pathobiology, School of Veterin... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411553 Fri, 11 Nov 2011 06:55:55 +0100 5411553 http://www.medworm.com/index.php?rid=5411554&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F780752081870tp15%2F Abstract  Nephrotoxicity results from an abrupt decline in glomerular functions of the kidney and is regarded as a major cause of concern for limiting therapeutic uses of gentamicin in hospital settings. Recent evidences suggest that both endothelial nitric oxide synthesis (eNOS) and inducible NOS (iNOS)-derived nitric oxide (NO) vasodilators reduce renal toxicity, restoring normal glomerular functions and hemodynamic stability, but this has not been adequately investigated in gentamicin nephrotoxicity. To determine the isoform of NOS that plays a predominant role in the control of glomerular vasodilation under the oxidative stress of nephrotoxicity, we conducted a controlled, randomized study in five equal groups of ten Sprague–Dawley rats. All animals were given a corres... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411554 Thu, 10 Nov 2011 06:46:35 +0100 5411554 http://www.medworm.com/index.php?rid=5411555&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6m48273061818x0u%2F Abstract  For economical and practical reasons, flank laparotomy performed on cattle is done while the animal is standing. To prolong the duration of surgical anesthesia with local anesthetic drugs, vasoconstrictors are commonly added to delay absorption and reduce the systemic toxicity. The purpose of this study was to examine the effects of lidocaine containing either 80 μg ml-1 norepinephrine or 12.5 μg ml-1 epinephrine on tissue integrity following flank inverted L infiltration anesthesia in sheep. Results of histologic examination of the skin and subcutaneous tissue sections by light microscopy showed significant pathologic changes (inflammatory changes and necrosis) of the epidermis and dermis in sheep receiving lidocaine containing 80 μg... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411555 Tue, 08 Nov 2011 06:48:00 +0100 5411555 http://www.medworm.com/index.php?rid=5411556&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5678070265186486%2F In this study, we aimed at optimizing culture conditions for obtaining insulin-producing cells from cord blood hematopoietic stem cells. Twenty cord blood samples were subjected to short-term, liquid static culture that favors the proliferation of CD34+ hematopoietic stem cells. A duplicate culture was set for each sample, one with high glucose concentration and the other with low glucose concentration. Then these cells were subsequently induced to transdifferentiate into insulin-producing cells via a biphasic liquid culture using exendin-4. The expression of human insulin was then tested using RT-PCR. At the end of the culture, 17 out of the 20 samples (85%) cultured in high glucose concentration showed positive human insulin mRNA expression, while culture media with low glucose con... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5411556 Tue, 08 Nov 2011 06:47:59 +0100 5411556 http://www.medworm.com/index.php?rid=5390981&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa22073j675511368%2F Abstract  The current study evaluated the effect of a standardized Crataegus extract on liver injury induced by acute carbon tetrachloride (CCl4) administration in rats. Crataegus extract (10, 20 or 40 mg/kg), silymarin (25 mg/kg) or saline (control) was given once daily orally simultaneously with CCl4 and for 1 week thereafter. Crataegus extract given at the above doses reduced serum alanine aminotransferase (ALT) levels by 28.8, 31.8 and 36.4%, respectively, when compared to the CCl4 control group. Serum aspartate aminotransferase (AST) levels decreased by 14.3, 15.5 and 20.6%, respectively, while alkaline phosphatase (ALP) decreased by 21.8% by the extract at 40 mg/kg. The administration of silymarin reduced ALT, AST and ALP levels by 66.8, 64.9 and 60%,... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5390981 Fri, 04 Nov 2011 16:56:20 +0100 5390981 http://www.medworm.com/index.php?rid=5390983&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb865j32v43802637%2F In this study, phylogenetic analysis showed that all 17 examined strains were clustered within the BVDV genotype 1. Particularly, 14 of them were clustered with the BVDV-1b subgroup, while the remaining three strains were clustered with the BVDV-1e. Moreover, the restriction analysis indicated a bovine origin for all of the 17 strains typed in this study. These results could be useful to carry out an epidemiological survey and to create vaccines that protect cattle against BVDV different subgroups. Content Type Journal ArticleCategory Original ArticlePages 1-6DOI 10.1007/s00580-011-1358-5Authors Vincenza Cannella, Department of Virology, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Palermo, ItalyElisabetta Giudice, Department of Veterinary Public Health, U... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5390983 Wed, 02 Nov 2011 16:55:49 +0100 5390983 http://www.medworm.com/index.php?rid=5390982&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu850q438gg87274p%2F In conclusion, because of the different physiological states of various bovine categories reared on Holstein dairy farms, mineral supplements (Cu, Zn and Fe) should be provided based on physiological requirements of individual bovine categories and the results of those diet analyses. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00580-011-1357-6Authors Vahid Noaman, Department of Veterinary Research, Isfahan Research Center for Agriculture and Natural Resources, P.O. Box 81785–199, Isfahan, IranMohsen Rasti, Department of Animal Science, Isfahan Research Center for Agriculture and Natural Resources, Isfahan, IranAhmad Reza Ranjbari, Department of Animal Science, Isfahan Research Center for Agriculture and Natural Resources, Isfahan, IranEdris Shirvan... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5390982 Wed, 02 Nov 2011 16:55:49 +0100 5390982 http://www.medworm.com/index.php?rid=5390985&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa7321l7878561p02%2F In this study, the first N. caninum isolate was considered low passage (no passage) and then passaged for 80 times in vitro as high passage. Groups of 8-day-old embryonated eggs with 10 eggs in each group were inoculated with 10 and 102 of low or high-passage N. caninum tachyzoites, and any mortality was recorded. Suitable samples from different tissues (liver, heart, and brain) of the dead embryos were collected for histopathological and immunohistochemistry (IHC) study. In this study, the mortality rate decreased in the high passage infection in each group and IHC method showed the presence of the parasite in chicken tissues receiving high and low passage parasites. The present investigation showed that the chicken embryonated eggs can be a valuable alternative approach for in vivo ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5390985 Wed, 02 Nov 2011 16:55:48 +0100 5390985 http://www.medworm.com/index.php?rid=5390984&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F318370n525173289%2F In conclusion, in aggressive types of septic arthritis, surgical drainage in addition to antibiotic therapy (as early as possible) is more reliable and safer than multiple needle aspirations and antibiotic therapy. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00580-011-1351-zAuthors Mohammad Jafar Emami, Research Center for Bone and Joint Diseases, Department of Orthopedic Surgery, Chamran Hospital, Shiraz University of Medical Sciences, Chamran Blvd, Shiraz, IranHesamaddin Fakhrinia, Research Center for Bone and Joint Diseases, Department of Orthopedic Surgery, Chamran Hospital, Shiraz University of Medical Sciences, Chamran Blvd, Shiraz, IranAzizollah Khodakaram Tafti, Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz-... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5390984 Wed, 02 Nov 2011 16:55:48 +0100 5390984 http://www.medworm.com/index.php?rid=5366927&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh667470401139807%2F This study aimed at elucidating the association of the gly972arg and the ala512pro polymorphisms of the IRS-1 gene with PCOS in Egyptian females. We employed a case–control study. The two polymorphisms were analyzed in 62 PCOS Egyptian female patients and 43 age-matched controls using a PCR-RFLP strategy. Fasting insulin, plasma glucose, and testosterone were analyzed by routine analytical methods and calculation of the homeostasis model assessment (HOMA) and the quantitative sensitivity check index (QUICK I) were done based on the results of the fasting insulin and glucose. It was found that 6.5% of the PCOS cases showed the Gly972Arg genotype. The Gly972Arg in IRS-1 polymorphism was not associated with PCOS (p = 0.556). A positive association was found between the gly972arg an... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5366927 Fri, 28 Oct 2011 17:18:34 +0100 5366927 http://www.medworm.com/index.php?rid=5366926&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr023787553h54rj3%2F This study aims to investigate variables suitable for monitoring of unfractionated heparin (UFH) therapy and establishment of an optimal therapy scheme in pigs. This is a prospective study of 32 pigs undergoing catheterization for endovascular embolization of experimentally induced arteriovenous malformation. Pigs were assigned to four groups receiving different UFH treatment during catheter intervention. In groups 1 and 2, UFH was applied as a bolus of either 100 IU/kg (n = 6) and 200 IU/kg (n = 6). Groups 3 and 4 received a continuous infusion of 66 IU/kg/h UFH (n = 10) and 100 IU/kg/h (n = 10), respectively, which was applied 20 min after an initial bolus of 100 IU/kg. Blood samples were taken 0, 10, 20, 40, 60, 100, and 1... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5366926 Fri, 28 Oct 2011 17:18:34 +0100 5366926 http://www.medworm.com/index.php?rid=5366928&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F127868l1w0008v8r%2F This study was performed using blood samples from eight healthy male ostriches, (3 months old), which were in good condition and clinically normal. Diurnal variation of serum biochemical parameters was determined by daily blood collections over 9 days in summer at the School of Veterinary Medicine, Shiraz University. In serum samples nonelectrolytes (total protein, albumin, glucose, cholesterol, triglyceride, HDL cholesterol, creatinine, urea nitrogen, and uric acid), electrolytes (calcium, inorganic phosphorus, sodium, and potassium), enzymes (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and creatin kinase (CK)), and hormones (triiodothyronine (T3) and thyroxine (T4)) were measured by validated standard methods. Most of the s... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5366928 Fri, 28 Oct 2011 17:18:32 +0100 5366928 http://www.medworm.com/index.php?rid=5366929&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg36ht6n235451422%2F We report a case with active systemic lupus erythematosus (SLE) who was treated successfully with plasma exchange (PE). A 26-year-old female, pregnant at 24 weeks, presented to the emergency room with vaginal bleeding, bleeding gums, facial rash, blurry vision, pallor, and lymphadenopathy. A diagnosis of SLE with secondary ITP was made based on her laboratory findings which revealed leucopenia, thrombocytopenia, evidence of hemolysis, positive antinuclear antibody, positive anti-double-stranded DNA antibody, positive lupus anticoagulant, false-positive VDRL, and decreased complement levels. High doses of corticosteroids were given when her illness was worsening with persistent hemolysis and thrombocytopenia. Plasma exchange was conducted after 2 weeks when she was not responding to t... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5366929 Fri, 28 Oct 2011 17:18:31 +0100 5366929 http://www.medworm.com/index.php?rid=5366930&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp7001378180023k7%2F This study examined the effects of combined administration of vitamins C and E to Plasmodium berghei-induced anaemia and organ weight alterations as well as changes in endogenous antioxidant status in mice. Three groups of mice were intraperitoneally infected with chloroquine-sensitive P. berghei (NK 65) among which a group was intraperitoneally treated with a combination of vitamins C (100 mg/kg body weight, bw) and E (1,000 i.u./kg bw) whereas another group was treated with chloroquine (25 mg/kg bw). The remaining infected group was left untreated. Data from these groups were compared with those from the uninfected group that was treated with vitamins C and E and the uninfected untreated group. Treatment with vitamins C and E did not significantly (P > 0.05) aff... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5366930 Fri, 28 Oct 2011 17:18:29 +0100 5366930 http://www.medworm.com/index.php?rid=5350369&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F738141qj85pg27k8%2F Abstract   Escherichia coli is a Gram-negative, nonspore-forming bacillus, usually motile, and a member of the family Enterobacteriaceae. Virulence factors such as CDT and CNF are associated with the capacity of E. coli strains to cause intestinal and extraintestinal infections, and sheep and mutton are important sources for transmission of this infection to human. The purposes of this study were to determine the presence of E. coli at the first step and to examine samples for detection of virulence genes using specific primers in Iranian sheep carcasses. One hundred fifty muttons were collected randomly from slaughterhouse industries in southwest Iran, and bacterial DNA was extracted. For gene amplification, PCR reaction was performed using 16srRNA primer for the presence of... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5350369 Fri, 21 Oct 2011 16:01:10 +0100 5350369 http://www.medworm.com/index.php?rid=5350370&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg61j77564w401203%2F Abstract  The present study was undertaken to evaluate hepatic antioxidants status in female mice after exposure to perfluorooctanoic acid (PFOA). A total number of 20 female mice were subjected to the study; out of them, 15 mice were treated with PFOA. PFOA solutions were prepared at 0.1, 0.5, and 1 mg/ml of deionized water and administered to the mice by gavage once daily for 3 weeks at a volume of 10 ml/kg. Controls (five mice) received an equivalent volume of deionized water. The results revealed significant increase in liver weight at dose of 5 (p < 0.01) and 10 mg/kg body weight (b.w., p < 0.01). Both hepatic total glutathione level and hepatic catalase activity were significantly increased (p < 0.01) at dose of 5 mg/kg b.w.... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5350370 Thu, 20 Oct 2011 15:53:47 +0100 5350370 http://www.medworm.com/index.php?rid=5338848&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F643358165l200077%2F The objective of this study was to investigate the immunohistochemical (IHC) detection of c-erbB2 receptor (crbP) and TP53, the co-expression of these two markers and their relationship with histological type and histological grade in canine mammary carcinoma. After histological diagnosis according to the WHO classification of 30 mammary carcinomas, their histologic grade was identified by the Elston and Ellis method for human breast tumours. Proliferation activity was detected by Ki67 protein. IHC staining was carried out for crbP and TP53 expression. Results of semiquantitative IHC assessment of crbP and 3+ TP53 expression were 23.3% and 53.3% of canine mammary carcinoma, respectively. There were significant relationships between Tp53 and histopathological grade and TP53 and Pki67.... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5338848 Mon, 17 Oct 2011 16:00:35 +0100 5338848 http://www.medworm.com/index.php?rid=5325886&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk22k2xu2127n8780%2F Abstract  Catheter interventions are associated with the risk of thromboembolism; however, the extent of platelet activation is not known. Samples from an arteriovenous malformation model (n = 21 pigs) were examined. The pigs received a continuous infusion of 66 IU kg−1 h−1 (n = 11) or 100 IU kg−1 h−1 (n = 10) heparin applied 20 min after an initial bolus of 100 IU/kg. Platelet aggregation according to Born and ADVIA 120™ platelet activation indices were used to study platelet function and activation. Samples were taken previous to vascular puncture, following vascular puncture, 20 min after application of heparin bolus, following placement of microcatheters and after endovascular embolization. Reactivity of p... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5325886 Fri, 14 Oct 2011 10:39:18 +0100 5325886 http://www.medworm.com/index.php?rid=5317315&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg525620471261713%2F Abstract  We assessed the levels of serum calcium (Ca), phosphorus (P) and magnesium (Mg) during the postpartum hours in adult Holstein-Friesian cows who received subcutaneous calcium borogluconate (CBG) post-calving. Fifteen cows were fed a 3-week close-up ration containing 63 g/day Ca and 40 g/day P, with a dietary cation–anion difference of 335 meq/kg DM. Within 30 min after calving, seven cows were injected 200 ml of a 40% CBG solution (6 g of Ca) subcutaneously and 8 cows were considered as controls. All cows were sampled from the jugular vein at calving time (before injection) and at hours 4 and 12 post-calving. Sera were analysed for Ca, P and Mg. In the experiment group, serum Ca remained almost constant within the physiologic lim... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5317315 Tue, 11 Oct 2011 15:52:26 +0100 5317315 http://www.medworm.com/index.php?rid=5317316&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8j0455t9tr255022%2F Abstract  Raini goat is an Iranian cashmere goat which is raised in large numbers in Kerman province of Iran where goat production contributes significantly to the agricultural economy. Normal hematological indices are valuable aid in the diagnosis of hematological disorders. Determination of the main hematological and hematochemical parameters of animals helps veterinarians to confirm clinical diagnosis, estimate the severity of cases, administer appropriate treatment, and evaluate outcomes. Limited information is available for normal hematological parameters in Raini goats. The purpose of the present study was to determine reference values of hematological indices for clinically healthy Iranian cashmere (Raini) goats in order to establish a basis for clinical interpretati... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5317316 Tue, 11 Oct 2011 15:52:24 +0100 5317316 http://www.medworm.com/index.php?rid=5306536&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp3m926u2m1g40455%2F According to this study, Echinacea has anti-inflammatory effects but may be acting as an agonist for arsenic, so using it for arsenic toxicity is not recommended. Content Type Journal ArticleCategory Original ArticlePages 1-4DOI 10.1007/s00580-011-1338-9Authors Marzieh Heidari, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranAnnahita Rezaie, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranMehdi Pourmahdi Broojeni, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranHossein Najafzadeh, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranBabak Mohammadian, Department of Pathobiology, Fa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5306536 Thu, 06 Oct 2011 05:49:45 +0100 5306536 http://www.medworm.com/index.php?rid=5298479&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv12p7jm373445651%2F In conclusion, there was a good correlation between these two diagnostic tests of fasciolosis although iELISA using ES Ag was more closely correlated with the histopathology findings and could be regarded as a rapid diagnostic method in bovine fasciolosis. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00580-011-1337-xAuthors Mohamad Hossein Razi Jalali, Pathobiology Department, Veterinary Medicine Faculty, Shahid Chamran, Ahvaz, IranSaleh Esmaeil Zadeh, Pathobiology Department, Veterinary Medicine Faculty, Shahid Chamran, Ahvaz, IranMasoud Ghorbanpoor Najaf Abadi, Pathobiology Department, Veterinary Medicine Faculty, Shahid Chamran, Ahvaz, IranJafar Arjmand Yamchi, Shahid Chamran University, Ahvaz, Iran Journal Comparative Clinical PathologyOnli... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5298479 Tue, 04 Oct 2011 05:49:33 +0100 5298479 http://www.medworm.com/index.php?rid=5298478&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm0221q0688u7k727%2F The objective of this study was to determine the prevalence of SCK in postpartum cows in Nishaboor, Iran. A total of 109 lactating cows in 2–4 weeks of lactation from 15 herds in Nishaboor were included in this study. Serum BHB and glucose concentrations were determined by BHB dehydrogenase and glucose oxidase methods. At the cutoff point of 1,400 μmol/L of BHB concentration, 18.42% (35/190) of dairy cows were considered subclinical ketotic with the herd range of 0–50%. The mean concentrations of glucose and BHB in ketotic were 30.6 ± 1.44 mg/dL and 2,400 ± 420 μmol/L, and in nonketotic cows were 43.6 ± 0.7 mg/dL and 637 ± 80 μmol/L, respectively. Blood glucose concentration in subclinical ketotic cows were significantly lower ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5298478 Tue, 04 Oct 2011 05:49:33 +0100 5298478 http://www.medworm.com/index.php?rid=5298480&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc80vu8l8j5256666%2F In this study, 144 1-day-old birds were divided in three groups of 48 animals each (non-treated group, Salmonella Typhimurium (ST)-inoculated group, and Lactobacilli inoculated group). A total of four blood collections were made with the first one performed 3 h after inoculation with ST or treatment with Lactobacilli. Subsequent samples were obtained every 48 h for 7 days. Leucometric evaluation was performed immediately after each collection. All birds presented an initial decrease pattern in general leukocyte values, and the chicks inoculated with ST revealed lymphomonoheteropaenic leukopaenia, eosinophilia and basophilia in conjunction with convalescence after 96 h of inoculation. The animals inoculated with Lactobacilli revealed leucocytosis with monocytosis, ly... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5298480 Tue, 04 Oct 2011 05:49:31 +0100 5298480 http://www.medworm.com/index.php?rid=5288140&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F147438066p732242%2F Abstract  Experimental tibial osteotomy was performed in 24 dogs, fixed by either intramedullary (IMO; n = 12) or plate and screw osteosynthesis (PLO; n = 12). Six dogs with IMO and another six with PLO were subjected to daily therapeutic ultrasound therapy at the site of osteotomy for 10 days, starting from the first post-operative day. The other dogs served as controls. Radiological studies were performed immediately after surgery, by week 2 and months 1, 2 and 3. Blood markers of bone formation (bone alkaline phosphatase and osteocalcin) and bone resorption (tartrate-resistant acid phosphatase and C-terminal telopeptide of collagen type I) were assayed before surgery, by post-operative weeks 1 and 2 and months 1, 2 and 3. Therapeutic ultras... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5288140 Sat, 01 Oct 2011 05:48:13 +0100 5288140 http://www.medworm.com/index.php?rid=5288141&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8884752652209581%2F Abstract  Values of some haematological indices were assessed in 30 Barbus grypus (mean length, 22.88 ± 4.16 cm; mean weight, 101.01 ± 58.50 g). The fishes were randomly selected from ponds of Dashte Azadegan Aquaculture Centre. The blood samples were collected from the caudal vein after adaptation to laboratory conditions. The values of the haematological parameter were measured using standardized and approved methods. The following values (mean±SD) were recorded. Packed cell volume (PCV), 46 ± 5.71%; haemoglobin (Hb), 9.5 ± 4.58 g/dL; white blood cell, 3,196.6 ± 716.54 in each mm3; red blood cell, 1,378,667 ± 195,408.2 in mm3, mean corpuscular haemoglobin concentration, 34.68 ± 20.23%; mean corpuscular haemoglobin 12.53 �... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5288141 Sat, 01 Oct 2011 05:48:12 +0100 5288141 http://www.medworm.com/index.php?rid=5252462&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa744371332488x42%2F Abstract  Diabetes mellitus is a disease that affects millions of people in the world. Many people use therapeutic herbal medicine for many reasons. In the present study, the effects of Ruta graveolens extract on the level of blood glucose, lipids, and hematological parameters have been studied. For this purpose 30 adult male Wistar rats weighing (200–300 g) were divided randomly into six groups (A, B, C, D, E, and F) and housed in single cages. The control group (A) was injected with normal saline. Diabetes was induced by injection of streptozotocin (60 mg/kg, i.p.) in other five groups. Group C received glibenclamide (10 mg/kg) orally, and groups D, E, and F received hydroalcoholic extract of R. graveolens (10, 20, and 30 mg/kg, i.p.) for 10 days... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5252462 Thu, 22 Sep 2011 05:52:07 +0100 5252462 http://www.medworm.com/index.php?rid=5240979&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F334157468398k588%2F Abstract  A total of 246 Japanese composed of 121 with benign prostatic hyperplasia (BPH) and 125 with prostatic cancer (PCa) were analyzed clinically to explain fusion gene anomalies between erythroblast transformation-specific family transcription factor-related gene (ERG) and transmembrane protease serine 2 (TMPRSS2) on chromosome 21q22. Among the 246 cases, 12 (10%) BPH and 18 (14%) PCa showed prostate-specific antigen (PSA) values of >20 ng/ml. It was shown that PSA was not only secreted from the prostates of BPH and PCa, but also actively from bone metastases, which occurred in the 18 PCa, although PCa bone metastases through lymphatic vessels were shown to be blocked by a cystic abscess formation or skin lipoma derived from lymphatic vessels. Among the 30 cas... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5240979 Mon, 19 Sep 2011 13:44:47 +0100 5240979 http://www.medworm.com/index.php?rid=5228662&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1866w728564618hx%2F Abstract  The antioxidant activity of Cassia singueana Delile (Fabaceae) Lock methanol leaf extract was investigated in vivo and in vitro using malondialdehyde (MDA) test, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and ferric reducing/antioxidant power (FRAP) photometric assays. The leaves have been of interest to researchers because of its use in the treatment of various disease conditions in Nigerian traditional medicine. C. singueana leaf extract gave a significant (P < 0.05) dose-dependent increase in antioxidant power with the FRAP assay. The DPPH assay showed 66% antioxidant activity at 400 μg/ml of the crude extract but ascorbic acid showed 79% at the same concentration. All doses (0.25, 0.5, and 1.0 g/kg) of the extract exhibited significant (P ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5228662 Thu, 15 Sep 2011 15:50:27 +0100 5228662 http://www.medworm.com/index.php?rid=5228663&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv44782360734077g%2F Abstract  One of the main parameters involved in the yeast-to-hypha transition in Candida albicans is temperature, and this change is involved in its pathogenicity. A complete switch between yeast and hypha can be achieved by changing the temperature from 28°C to 37°C in Lee medium supplemented with serum. To compare the early transcriptional response of C. albicans to temperature, we have carried out a genome-wide analysis of the C. albicans response to temperature after a 5-min exposure at 37°C. Using a cDNA microarray method, we found changes in 1,635 genes, suggesting that the key time for controlling the dimorphic change occurs very early. The overrepresented categories of up-regulated genes consisted of transporters, transcription factor and translation initiation fa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5228663 Tue, 13 Sep 2011 15:44:34 +0100 5228663 http://www.medworm.com/index.php?rid=5212522&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc6855741nm1un633%2F Abstract  The aim of the study was to evaluate baseline data of mutant strain of mice and to compare haematological parameters with their background strains. Almost all mutant strains of either sex had statistically significant changes in relation to their background strains. These findings will be useful for researchers in designing experiments on mutant strains for various disease models and interpreting data obtained from those strains. Content Type Journal ArticleCategory Original ArticlePages 1-9DOI 10.1007/s00580-011-1330-4Authors Perumal Nagarajan, Small Animal Facility, National Institute of Immunology (NII), New Delhi, IndiaArindkar Shailendra, Small Animal Facility, National Institute of Immunology (NII), New Delhi, IndiaRamasamy Venkatesan, Animal House, JNU,... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5212522 Fri, 09 Sep 2011 05:49:08 +0100 5212522 http://www.medworm.com/index.php?rid=5212523&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe2501679j2767742%2F Abstract  A 2-year-old male mixed breed dog was presented with history of a slow growing subcutaneous mass on the back near to left hip joint. Grossly, a large, firm, gray-white mass about 4.5 × 3.5 × 7.5 cm in dimensions was located within the dermis. Microscopically, a poorly encapsulated, multilobular tumoral tissue was observed with proliferation of the glandular epithelium as tubular and solid structures. The proliferating epithelial cells lining the tubules had round to ovoid, normochromatic nuclei with prominent nucleoli and relatively large eosinophilic cytoplasm. The lumens of a number of tumoral tubules were distended by eosinophilic secretions as hyaline casts. In immunohistochemistry, immunoreactivity for the S-100 protein and cytokeratin were dif... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5212523 Thu, 08 Sep 2011 05:51:32 +0100 5212523 http://www.medworm.com/index.php?rid=5198457&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F72767nu2g60502h0%2F This study investigated the testicular morphology as well as the gonadal and extra-gonadal sperm reserves of West African Dwarf (WAD) goats exposed to graded levels of 2,4-dichlorophenoxyacetic acid (2,4-D). Twenty male WAD goats of five goats per group were used for this study. Goats in groups A, B and C received low [75 mg/kg body weight (BW)], medium (100 mg/kg BW) and high (125 mg/kg BW) dose levels of 2,4-D, respectively. The group D goats served as the control. On day 112, goats in the four groups were sacrificed and the testicular and epididymal sperm reserves were determined. Histopathologic changes in the testis of the 2,4-D-exposed and control goats were also assessed. The mean number of spermatozoa in the testes and the various segments of the epididymi... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5198457 Mon, 05 Sep 2011 16:13:47 +0100 5198457 http://www.medworm.com/index.php?rid=5198459&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6138x86563485w72%2F Abstract  There is a discrepancy between the knowledge of veterinary medicine graduates and professional and society expectations. The main reason for this discrepancy is the conventional curricula, which rely on backward reasoning or disease-centered medical education. The aim of the present study was to develop a scheme as the most important step in the creation of a full clinical presentation (CP) module. Vomiting was chosen in this study as a common CP in dogs. Three series of focused questions were developed. The physiological mechanisms (visceral, cortical, vestibular, metabolic, and chemical) were chosen to reconstruct the top layer of the scheme. To reconstruct the second and third layers of the scheme, anatomical concepts were applied to define the subcategories. T... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5198459 Mon, 05 Sep 2011 16:13:46 +0100 5198459 http://www.medworm.com/index.php?rid=5198458&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx158789568047238%2F This study was aimed to evaluate the preventive efficacy of syringic acid (SA) on acetaminophen (APAP)-induced nephrotoxicity. Nephrotoxicity was induced in male Wistar albino rats by the administration of a single dose of 750 mg/kg APAP intraperitoneally. In this study, we evaluated the levels of renal markers, lipid peroxidation markers (thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH)), enzymatic and nonenzymatic antioxidant status, and histopathological changes of kidney. Our results show significant (P < 0.05) increased levels of serum urea, uric acid, creatinine, and TBARS, LOOH in the kidney tissue. In addition, the activities of superoxide dismutase, catalase, glutathione peroxidase, and reduced glutathione levels significantly (P <... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5198458 Mon, 05 Sep 2011 16:13:46 +0100 5198458 http://www.medworm.com/index.php?rid=5198460&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd325577580254w25%2F Abstract  Concurrent outbreak of staphylococcal pneumonia with infectious bursal disease (IBD) occurred in 29-day-old broiler chickens. The outbreak started after a flock of 279 birds was decongested by moving 121 birds to another pen. The birds were depressed and weak before death and carcasses were cyanotic. At necropsy, the lungs were dark red, congested and consolidated; the bursa of Fabricius (BF) was enlarged; the liver was pale and the intestinal mucosa was hyperaemic. Histopathological examination of the lungs, BF and liver revealed fibrinopurulent pneumonia, purulent inflammation of the BF and hepatic lipidosis, respectively. Staphylococcus aureus was isolated from the teased tissue of the lungs. The mortality was 35.8% within 5 days of the outbreak. The circum... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5198460 Mon, 05 Sep 2011 16:13:45 +0100 5198460 http://www.medworm.com/index.php?rid=5193048&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd081668400126212%2F Abstract  The study of canine haemoglobin (Hb) components can help to forecast Hb changes during many pathological and physiological processes such as responsive anaemia. The aims of our study were to show canine Hb electrophoretic pattern on cellulose acetate and identify Hb types similar to the human Hb pattern. Blood samples from 78 different canine breeds were randomly collected in tubes containing anticoagulant EDTA. Animals were brought to the Small Animal Teaching Centre for a check-up and vaccination. All blood samples underwent electrophoresis on cellulose acetate paper strips to determine Hb types. Haematocrit and Hb were measured simultaneously. The Hb electrophoresis results showed that Hb A1 was assigned to most of Hb components on cellulose acetate paper. Also,... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5193048 Fri, 02 Sep 2011 05:46:27 +0100 5193048 http://www.medworm.com/index.php?rid=5171645&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd883020234153k54%2F This study investigated the leucocytic profile of rats experimentally infected with T rypanosoma brucei brucei and treated with a combination of methanolic Azadirachta indica leaf extracts (MAILE) plus diminazene diaceturate (DDA). Acute toxicity study of the drug and extract combinations was carried. Selection of the best drug and extract combinations was carried out using 54 rats of both sexes separated into nine groups. Three dose combinations were derived from the selection of the best drug and extract combinations used for the final study viz, 7 mg/kg body weight (bw) DDA plus 125 mg/kg bw extract (group B), 3.5 mg/kg bw DDA plus 250 mg/kg bw extract (group C) and 1.8 mg/kg bw DDA plus 500 mg/kg bw extract (group D). The final study had in addition t... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5171645 Thu, 25 Aug 2011 15:47:29 +0100 5171645 http://www.medworm.com/index.php?rid=5171644&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw7005557k6713675%2F The objective of the present work was to investigate the effects of buprenorphine administration on testicular tissue in mice. Sexually mature male mice were randomly divided into five experimental groups (n = 5) and the following treatments were intraperitoneally administered for 21 days: Control male mice were given physiological saline. Positive control group was injected with morphine (Mor, 20 mg/kg). Three other groups were given different doses of buprenorphine including 0.15 mg/kg (B15), 0.30 mg/kg (B30), and 0.60 mg/kg (B60). Left testes were removed for histopathological evaluations and intracardiac blood samples were taken for testosterone assay. Morphometrically, the mean of seminiferous tubules diameter, spermatogonial cells diameter, sertoli c... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5171644 Thu, 25 Aug 2011 15:47:29 +0100 5171644 http://www.medworm.com/index.php?rid=5171648&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu60v117588p8138l%2F This study was conducted on 155 neonates aged 3–7 days attending the neurometabolic clinic of Cairo University Children Hospital as a part of the neonatal screening program. Samples of patients were selected according to their phenylalanine levels obtained by MS/MS. Group 1 included 49 normal neonates with phenylalanine level <150 μmol/L. Group 2 included 106 phenylketonuria (PKU)-affected neonates with phenylalanine level >150 μmol/L selected to cover the analytical range of the commercial kit. Blood samples on filter paper cards were reassayed by an enzyme-based colorimetric test kit. On correlating the phenylalanine level assayed using enzyme-based colorimetric technique using Enzolve kit and MS/MS technique, r = 0.83 and P < 0.01 were found. Al... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5171648 Thu, 25 Aug 2011 15:47:27 +0100 5171648 http://www.medworm.com/index.php?rid=5171647&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4r12621531278174%2F Abstract   Echinacea preparations are widely used in the prevention or treatment of upper respiratory tract infections. The present study aimed to investigate the effect of a standardized Echinacea extract in experimentally induced liver toxicity and whether this herb would have a modulating effect on the silymarin-induced hepatoprotection in rats. Liver damage was induced by the administration of carbon tetrachloride (CCl4). Echinacea extract (18 or 36 mg/kg) alone or combined with silymarin (25 mg/kg), silymarin only (25 mg/kg), or saline (control) was given once daily orally simultaneously with CCl4 and for 1 week thereafter. Serum alanine aminotransferase (ALT) or aspartate aminotransferase were not significantly changed by treatment with Echinacea, b... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5171647 Thu, 25 Aug 2011 15:47:27 +0100 5171647 http://www.medworm.com/index.php?rid=5171646&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F452n3w1820j01604%2F The objectives of the present study were to describe radiographic and magnetic resonance imaging (MRI) findings of early osteoarthritis and their relationships at the same time. A total number of ten rabbits were used and randomly divided into two equal groups. In one group, cranial cruciate ligament (CCL) of the left knee was transected to produce experimental osteoarthritis and in the other group, only the articular capsule was entered as sham operation (arthrotomy group). All of the left knees were examined by MRI and radiography before operation to exclude preexisting abnormalities and serve as control. One month postoperation, two conventional radiographic views and MR imaging were performed under general anesthesia. Statistical analysis of the MRI results revealed that there wa... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5171646 Thu, 25 Aug 2011 15:47:27 +0100 5171646 http://www.medworm.com/index.php?rid=5164987&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg5p526v30hu54h42%2F Abstract  Gender-related changes in plasma chemistry values were examined in guinea pigs after taking oral fluoxetine hydrochloride (HCl) as a widely prescribed drug for weight reduction and treatment of depressive disorders. Twenty guinea pigs of both sexes were divided into four groups of five animals. For both of the sexes, one group was treated with oral fluoxetine HCl (20 mg/kg/day) and another was used as a control which administered with distilled water. On the last day of the experiment (35th day), blood samples were collected for determining selected plasma biochemical parameters. For lipid profile, increases in high-density lipoprotein cholesterol, very low-density lipoprotein-cholesterol, and triglycerides were seen in both sexes, along with increases in tot... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5164987 Tue, 23 Aug 2011 15:52:22 +0100 5164987 http://www.medworm.com/index.php?rid=5164988&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F862q64030727408w%2F Abstract  Hematological parameters are repeatedly used as an essential diagnostic tool to assess the health condition of fish. The purpose of this study was to assess the reference values of some hematological parameters of Catla, Catla catla collected from a freshwater pond in a tropical climate of India from July 2008 to June 2010. Variations in hematological parameters such as hemoglobin, erythrocyte count, total leucocyte count, hematocrit, mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) of fish were compared according to sex and seasonal differences. Analysis of variance showed that there were significant differences between sexes and the results indicated that blood parameters levels between the sexes in summer were significantly dif... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5164988 Tue, 23 Aug 2011 15:52:21 +0100 5164988 http://www.medworm.com/index.php?rid=5149063&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F97802672072317m1%2F Abstract  The aim of the study is to resolve the debated association between ghrelin level and polymorphism of ghrelin gene (Leu72Met) with obesity and type 2 diabetes mellitus. The present study was conducted on 30 diabetic middle-aged patients with normal body mass index (BMI) and 40 age-matched non-diabetic participants (20 with normal BMI and 20 obese). All participants were subjected to full medical history and examination, anthropometric measurements, and laboratory investigations which include complete lipid profile, fasting plasma glucose, glycated hemoglobin, fasting serum insulin and HOMA-IR was calculated, fasting plasma ghrelin level was determined using ELISA method, and the Leu72Met polymorphism of the ghrelin gene was screened using PCR-RFLP. The median level... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5149063 Mon, 22 Aug 2011 16:05:28 +0100 5149063 http://www.medworm.com/index.php?rid=5149064&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv2v76m8322575673%2F Abstract  Peritonitis is an inflammation of the peritoneal cavity and is one of the main causes of animal deaths. It has been reported that many diseases such as peritonitis cause electrolyte imbalance in the body. The present study has been conducted to evaluate the serum electrolyte concentration in cattle with peritonitis. In order to perform this study, 45 cattle with peritonitis were selected in the Karaj area, and 20 healthy cattle were used as the control group. After diagnosis of peritonitis in the infected cattle, 10-ml blood samples were taken from the jugular vein, the concentrations of calcium, phosphorus, magnesium, and chloride were estimated using the spectrophotometric method, and sodium and potassium concentrations were assessed by a flame photometer device... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5149064 Thu, 18 Aug 2011 06:02:37 +0100 5149064 http://www.medworm.com/index.php?rid=5134190&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7486v19463868761%2F Abstract  An 8-year-old male Doberman was presented with a unilateral enlarged mass on its left upper eyelid. Neoplasia was suspected, and a series of diagnostic tests were carried out. Unrecognizable cells were seen in a fine needle aspiration that resembled a neoplastic lesion. The mass was resected from the base with some of underlying tissue, and several samples were obtained for histopathologic examination. Histopathologically, the tumoral mass was composed of a solid growth of irregular islands and trabeculae of basaloid reserve cells, interspersed with fewer sebocytes. The basaloid cells showed moderate mitotic activity. Based on clinical and histopathological findings, meibomian gland epithelioma was diagnosed. This is the first reported case of meibomian epitheliom... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5134190 Sat, 13 Aug 2011 06:05:22 +0100 5134190 http://www.medworm.com/index.php?rid=5134191&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn4433275g50l2747%2F In this study the antibacterial effects of essential oil extracted from Myristica fragrans, Zataria multiflora Boiss, Syzygium aromaticum, and Zingiber officinale Rosci, alone and in combination with nisin, were evaluated against Listeria monocytogenes in brain heart infusion broth. Minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) with essential oils and pH values (5, 6, and 7), alone and in combination with nicin (5 μg ml−1), were determined. The individual essential oils all showed antibacterial effects against this bacterium. Combination of essential oils and nisin showed synergetic effects such that when the essential oils were utilized in combination with nisin, MIC and MBC decreased. By decreasing pH, these antibacterial effects were i... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5134191 Sat, 13 Aug 2011 06:05:20 +0100 5134191 http://www.medworm.com/index.php?rid=5112289&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3314j5p8v7648674%2F Abstract  To identify the features of experimental toxoplasmosis in broiler chickens (Gallus domesticus), a total of 48 birds aged 25 days were randomly assigned to one of four groups of 12 birds each. Tachyzoites of Toxoplasma gondii were injected intraperitoneally at doses of 5 × 105 (group A), 1 × 106 (group B) and 1.5 × 106 (group C), and chickens in group D were treated with an injection of saline only (control). Before and after experimental infection, serum samples from all chickens were tested for antibodies against T. gondii with the Sabin–Feldman reaction. After infection, the clinical signs in all the chickens were recorded daily, and blood smears were prepared to determine parasitemia. Paraffin-embedded tissue sections were used for semi-nested... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5112289 Sat, 06 Aug 2011 05:59:34 +0100 5112289 http://www.medworm.com/index.php?rid=5112290&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F96730876u7613200%2F In this study, the coronary veins of the sheep heart were blocked experimentally and haematological and inflammatory indices (haptoglobin, serum amyloid A, tumour necrosis factor-α and interferon-γ) alterations were studied. Twenty sheep were used for this study. Anaesthesia was induced by ketamine and maintained using halothane. The intercostal space between ribs 4 and 5 was incised, the thorax was opened and the pericardium was incised. In the control group (1), only the myocardium was manipulated. In the second group, three to four superficial coronary veins were totally ligated. In the third group, three to four superficial coronary veins were partially ligated. In the fourth group, five to ten superficial coronary veins were totally ligated. In the fifth group, five to ten sup... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5112290 Sat, 06 Aug 2011 05:59:33 +0100 5112290 http://www.medworm.com/index.php?rid=5099431&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa32m768xl4713877%2F In this study, we characterized six ABC transporter proteins immediately downstream of a sigma factor as AsbI, which were denominated AsbH, AsbJ, AsbK, AsbL, AsbQ, and AsbM, and a siderophore receptor as AsbE. All studied genes showed a high similarity with other siderophore transporter genes in other gram-negative bacteria. Interestingly, asbH and asbJ genes were shown to encode a mixed-permease, ATP-binding protein. Reverse transcriptase PCR analysis demonstrated that the gene cluster is arranged into two transcriptional units: (1) asbIHJKLQM and (2) asbE. It also showed that the expression of these genes is regulated by iron concentration. Content Type Journal ArticlePages 1-5DOI 10.1007/s00580-011-1310-8Authors Mohsen Najimi, Department of Pathobiology, Faculty of Veterinary... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5099431 Tue, 02 Aug 2011 06:17:14 +0100 5099431 http://www.medworm.com/index.php?rid=5099432&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F10848v69417p8811%2F Abstract   Citrullus colocynthis seeds are traditionally used as antidiabetic medication in Mediterranean countries. An experiment was designed to evaluate the comparative effects of oral administration of C. colocynthis and insulin injection on the serum biochemical parameters of diabetic dogs. Twelve apparently healthy mixed breed dogs were selected and randomly allocated into three groups, two diabetic groups and one control group (n = 4). Diabetes was induced with alloxan tetrahydrate. One diabetic group and the control group were treated with a dose of 100 mg/kg/day of C. colocynthis. Three days after confirmation of diabetes mellitus, the dogs were orally administered C. colocynthis capsules twice daily for 8 days. The other diabetic group was injected wit... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5099432 Tue, 02 Aug 2011 06:17:13 +0100 5099432 http://www.medworm.com/index.php?rid=5099434&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg331123311735474%2F Abstract  The anti-diabetic effect of the drug Semecarpus anacardium Linn nut milk extract (SA) was studied in male Sprague Dawley rats. The rats were divided into seven groups of six animals each. Type 2 diabetes mellitus was induced in rats by feeding them with a high fat diet for 2 weeks followed by intraperitoneal injection of 35 mg/kg/body weight (b. wt.) of streptozotocin. The diabetic rats were treated with the drug at four different concentrations of 100, 200, 300 and 400 mg/kg/b. wt. respectively and were compared with the diabetic rats treated with the standard drug metformin for 30 days. Diabetic rats showed an increase in blood glucose levels, increased levels of glycosylated haemoglobin (HbA1c) and impaired glucose tolerance and an increase i... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5099434 Tue, 02 Aug 2011 06:17:12 +0100 5099434 http://www.medworm.com/index.php?rid=5099433&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq36t00v607532648%2F In conclusion, these results shed light on the composition of serum proteins of cows during a 1-year study and allowed us to delineate a serum pattern during physiological conditions. Content Type Journal ArticlePages 1-5DOI 10.1007/s00580-011-1311-7Authors Giuseppe Piccione, Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, Polo Universitario Annunziata, University of Messina, 98168 Messina, ItalyVanessa Messina, Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, Polo Universitario Annunziata, University of Messina, 98168 Messina, ItalyDaniela Alberghina, Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, Polo Universitario Annunziata, University of ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5099433 Tue, 02 Aug 2011 06:17:12 +0100 5099433 http://www.medworm.com/index.php?rid=5081963&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn7542w81715t72r2%2F This study investigated the erythrocytic profile of rats experimentally infected with Trypanosoma brucei brucei and treated with a combination of methanolic leaf extract of Azadirachta indica and diminazene diaceturate (DDA). Acute toxicity study of the drug and extract combinations was carried; selection of the best drug and extract combinations was carried out using 54 rats of both sexes separated into nine groups. Three dose combinations were derived from the selection of the best drug and extract combinations used for the final study, viz: 7 mg/kg body weight (bw) DDA plus 125 mg/kg bw extract (group B), 3.5 mg/kg bw DDA plus 250 mg/kg bw extract (group C) and 1.8 mg/kg bw DDA plus 500 mg/kg bw extract (group D). The final study had, in addition to the... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081963 Fri, 29 Jul 2011 16:05:53 +0100 5081963 http://www.medworm.com/index.php?rid=5081964&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F62342556w068q444%2F This report emphasizes the clinical course, the sequential hematological abnormalities, the cytology and the unusual immunophenotype of a presumptive histiocytic sarcoma in a cat. Content Type Journal ArticlePages 1-6DOI 10.1007/s00580-011-1304-6Authors Mathios E. Mylonakis, Companion Animal Clinic, Veterinary Faculty, Aristotle University of Thessaloniki, Thessaloniki, GreeceNektarios Soubasis, Companion Animal Clinic, Veterinary Faculty, Aristotle University of Thessaloniki, Thessaloniki, GreeceMaria Kritsepi-Konstantinou, Diagnostic Laboratory, Veterinary Faculty, Aristotle University of Thessaloniki, Thessaloniki, GreeceWilliam Vernau, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USAKonstantina Theodor... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081964 Fri, 29 Jul 2011 16:05:52 +0100 5081964 http://www.medworm.com/index.php?rid=5081965&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv76k755124u05657%2F This study was conducted on 30 patients with Child A, B, and C grades of chronic liver disease (ten patients for each stage) as well as ten age- and sex-matched normal healthy subjects were enrolled as a control group. The percent of circulating HSCs was determined by flow cytometry. Also, the isolation of such cells was done by magnetic cell-sorting technique for further ultrastructural assessment by transmission electron microscopy (TEM). Our study revealed that chronic liver disease patients compared to healthy control group, exhibited insignificant difference in the percentage of circulating CD133+ cells. Regarding the level of CD34+ cells, a significant increase was found between Child A chronic liver disease patients and the control group (p = 0.02). However, an insignificant... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081965 Thu, 28 Jul 2011 15:58:00 +0100 5081965 http://www.medworm.com/index.php?rid=5081967&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk411080884324g0x%2F Abstract  An outbreak of mycoplasmal pneumonia occurred in a flock of 180 head of sheep. The prominent clinical signs included pyrexia, coughing, respiratory distress, nasal discharge, ocular opacity in some cases, hot and swollen joints, lameness, and depression. Routine post-mortem examinations were performed on 11 dead sheep. The gross lesions included reddening, consolidation, and localized necrosis in different areas of the lung, the presence of a fibrinopurulent membrane on the pleural surface, and serofibrinous fluid in the thoracic cavity. Culture of the pneumonic lungs, liver, thoracic lymph nodes and nasal swabs in liquid and solid media yielded mycoplasma which was microscopically visible on solid media after 24 h at 37°C. Microscopically, pulmonary lesions... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081967 Thu, 28 Jul 2011 15:57:59 +0100 5081967 http://www.medworm.com/index.php?rid=5081966&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu2416824jn0456x2%2F This study concluded that theileriosis in sheep was associated with some alterations in blood parameters which could be useful in the diagnosis of ovine theileriosis. Content Type Journal ArticlePages 1-4DOI 10.1007/s00580-011-1308-2Authors Hasan Baghshani, Department of Basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranGholam Reza Razmi, Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranSaeed Yaghfouri, Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranAmin Ahmadi Dezaki, Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Journal Comparative Clinical PathologyOnline ISSN 16... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081966 Thu, 28 Jul 2011 15:57:59 +0100 5081966 http://www.medworm.com/index.php?rid=5081968&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft884236734274604%2F Abstract  The purpose of this study is to determine reference ranges for hematological and plasma chemistry of Psammophilus blanfordanus (Sauria: Agamidae) collected from their natural habitat. Blood samples were taken from coccygeal vein and lower abdominal vein of P. blanfordanus and different blood parameters were measured. Among the hematological parameters, there was sex wise difference in RBC count between males and females. Measurement of various types of blood cells was carried out with the help of a stage and an ocular micrometer. Erythrocytes were oval or elliptical shape both in male (length 15.375 ± 1.376 μm, breadth 10.75 ± 0.957 μm) and female (length 12.83 ± 3.083 μm, breadth 7.975 ± 2.206 μm) with centrally located o... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081968 Thu, 28 Jul 2011 15:57:58 +0100 5081968 http://www.medworm.com/index.php?rid=5081969&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F611265760410776p%2F Abstract  Several strategies and methods have been attempted to purify K99 fimbriae from enterotoxigenic Escherichia coli strains. Most of these methods are technically complex and time consuming. In current study a single-step ion-exchange chromatography method that takes just a few hours for purification of this important virulence factor was developed. K99 fimbriae were stripped from E. coli B41 strain by heat treatment and phosphate–urea buffer. The crude extracts were then equilibrated with Tris buffer and were loaded to a HiTrap SP XL column. By stepwise elevation of sodium chloride concentration, the FanC was eluted as a single species. The purified protein had high activity with anti-K99 monoclonal antibody in ELISA. Also, preincubation of red blood cells with the ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081969 Tue, 26 Jul 2011 15:48:47 +0100 5081969 http://www.medworm.com/index.php?rid=5081970&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1741381x1m447471%2F Abstract  The pathophysiology of non-Hodgkin’s lymphoma is still unknown. Many cytokines, including interleukin-10 (IL-10), play a role in the perpetuation of the disease. The aim of the study was to investigate the association of IL-10 gene promoter polymorphisms with non-Hodgkin lymphoma and to correlate with survival. Fifty patients with diffuse large B-cell lymphoma as well as 50 age- and sex-matched apparently healthy volunteers were genotyped for biallellic IL-10 gene promoter polymorphisms at positions −1082(A/G) and −3557(T/A) using polymerase chain reaction–restriction fragment length polymorphism. There were highly statistically significant differences between the two studied groups regarding results of IL-10 1082A/G polymorphism, for homozygous (GG) and h... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081970 Tue, 26 Jul 2011 05:53:58 +0100 5081970 http://www.medworm.com/index.php?rid=5081972&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fpn357l1w44777m66%2F Abstract  A direct association between trace elements and human disease has been observed. The alteration of chromium metabolism has been observed in diabetic patients. The aim of this study was to investigate any association between serum chromium levels and diabetes mellitus in the Egyptian population. Thirty patients with type 2 diabetes were compared to 20 healthy age- and sex-matched control subjects as regards serum chromium levels (measured on atomic absorption spectrometry), fasting and postprandial blood glucose, HbA1C, T.C., T.G., HDL-C, and LDL-C. Serum chromium levels were significantly lower in diabetic patients than in the control subjects (p = 0.035). There was a significant increase in T.C., T.G., and LDL-C in the patient group than the control group (p�... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081972 Tue, 26 Jul 2011 05:53:57 +0100 5081972 http://www.medworm.com/index.php?rid=5081971&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp7334064l42l346t%2F Abstract  In order to establish baseline hematological and biochemical values in loggerhead turtles from the Mediterranean Sea, 84 specimens were sampled, comprising 24 wild turtles in good health at the time of capture and 60 turtles tested after indoor rehabilitation at the Sea Turtle Rescue and Rehabilitation Centre of the Zoological Station Anton Dohrn in Naples, Italy. The following parameters were evaluated: red cell counts (RBC, 488–575 × 103/μL), white cell counts (WBC, 17–24 × 103/μL) and thrombocyte counts (TBC, 19–49 × 102/μL), hemoglobin (Hb, 8–14 g/dL), hematocrit (Ht, 23–34%), mean corpuscular volume (MCV, 487–723 fL), mean corpuscular hemoglobin (MCH, 170–261 pg), mean corpuscular hemoglobin concentration (MCHC, 34... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081971 Tue, 26 Jul 2011 05:53:57 +0100 5081971 http://www.medworm.com/index.php?rid=5081973&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7p0840n3x360l385%2F Abstract  Enterococci have been incriminated as a cause of opportunistic infections in humans and different animal species. The glycopeptide-resistant Enterococcus raffinosus has been reported as an emerging pathogen in humans recently, but the importance of E. raffinosus in animals is largely unknown. The present manuscript appears to be the first report of isolation of E. raffinosus from animal source; more importantly, the isolated organism showed resistance to vancomycin in conventional antibiotic resistance evaluation methods. The organism was isolated from a 35-day-old ostrich chick suffering from diarrhoea in pure culture. Public health and clinical significance of this emerging pathogen need further attention in veterinary and human medicine. Content Type Journal ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5081973 Tue, 26 Jul 2011 05:53:55 +0100 5081973 http://www.medworm.com/index.php?rid=5072448&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj422657514508v7r%2F Abstract  The effect of the methanolic extract of Mucuna pruriens seed was evaluated in mice with regards to delayed hypersensitivity reaction (DTR), primary and secondary antibody response and in vivo inflammatory leucocyte mobilization. The extract at 250 and 500 mg/kg produced significant (p < 0.05) inhibition of DTR in mice by 33.33% and 28.89%, respectively. The extract caused elevation of secondary SRBCs-specific antibody titre with antibody response being significantly (p < 0.05) increased at 250 and 500 mg/kg when compared with control. The extract at 250 and 500 mg/kg increased leucocyte mobilization significantly (p < 0.05) when compared with the control and levamisole-treated groups. At 100 mg/kg, the mobilization did not dif... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5072448 Mon, 25 Jul 2011 15:44:21 +0100 5072448 http://www.medworm.com/index.php?rid=5072447&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk77h8g1263388768%2F In this study, we discuss gross, radiological and histopathological features of an oral SCC in a female sheep. A 3-year-old ewe with a progressive mass located in the intraoral cavity and with a history of reduced appetite, weight loss, salivation and halitosis was referred to the veterinary clinic. At clinical examination, a mass was located in the floor of the mouth, its surfaces showed signs of ulceration and haemorrhage. Lateral radiograph of the mandible region near the tumour showed bone destructive changes. Biopsy specimen was taken and histopathologic examination showed moderately differentiated oral SCC. Content Type Journal ArticlePages 1-4DOI 10.1007/s00580-011-1301-9Authors Abdul Rasool Namjoo, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5072447 Mon, 25 Jul 2011 15:44:21 +0100 5072447 http://www.medworm.com/index.php?rid=5072449&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh586132357k07432%2F This study attempted to characterize and ascertain the role of a siderophore ABC transporter protein AsbJ in siderophore transporter system in this bacterial pathogen. Prediction of protein domains was carried out, and deleted asbJ gene mutant strain was constructed. Results showed that this protein contains special domain related to permease and ATP-binding protein, and it is essential for the siderophore transport process in this bacterial fish pathogen. Content Type Journal ArticlePages 1-5DOI 10.1007/s00580-011-1292-6Authors Mohsen Najimi, Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Journal Comparative Clinical PathologyOnline ISSN 1618-565XPrint ISSN 1618-5641 (Source: Comparative Clinical Pathology) Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5072449 Mon, 25 Jul 2011 15:44:20 +0100 5072449 http://www.medworm.com/index.php?rid=5058646&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff743335925630584%2F Abstract  Toxigenicity, species, and type species of Pasteurella multocida isolates cannot be differentiated by morphology or standard biochemical reactions. A more rapid method is needed for P. multocida detection from clinical cases. These findings provide rapid insights into the characteristics of P. multocida isolates and suggest that this method can identify toxigenic and specific capsular type P. multocida. A PCR assay has been developed for rapid detection of P. multocida and differentiation of capsular types A and D. In this rapid method, kmt1, capA and capD, and toxA genes were amplified and a reliable multiplex PCR method for the detection of P. multocida in sheep and goats in the south of Iran was designed. Twenty isolates were obtained, which evinced characteristic mo... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058646 Thu, 21 Jul 2011 18:03:56 +0100 5058646 http://www.medworm.com/index.php?rid=5058647&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmt351154m4053v71%2F Abstract  Clonal abnormalities are identified by standard cytogenetic analysis of bone marrow cells in 40–50% of individuals with acquired idiopathic sideroblastic anemia/refractory anemia with ringed sideroblasts, a distinctive hematologic illness which is also classified as a form of myelodysplasia. Certain recurring chromosomal abnormalities, including 5q-, 7q-, 20q-, and trisomy 8, are the most frequent examples. Isolated trisomy 19 as a sole clonal aberration in this disorder is a rare event. The case presented here, and which demonstrated 30% involvement of bone marrow cells with trisomy 19, as quantified by fluorescent in situ hybridization studies, appears to represent only the fifth reported instance of such association. An atypical feature in this case was the u... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058647 Thu, 21 Jul 2011 18:03:55 +0100 5058647 http://www.medworm.com/index.php?rid=5058648&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv120477104r4lk75%2F This study was conducted to identify the possible correlation between zinc deficiency and excessive shedding of hair in cats. Two male cats aged 2 and 3 years old, Persian breeds, and weighing 2.9 and 3.2 kg were presented to the Veterinary Hospital of Shahid Chamran University with chief complaints of excessive shedding of hair, depressed appetite, and weight loss in the past month. Physical examination revealed a relatively weak body condition. Serum zinc, copper, calcium, and iodine concentrations were measured using commercially available kits (Randox Laboratories, UK) with a spectrophotometer (Shimadzu, UV-1601). Total protein concentrations were assessed with a refractometer also. A decrease in zinc concentrations (35 and 41 μg/dl) was observed in the studied ca... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058648 Thu, 21 Jul 2011 18:03:53 +0100 5058648 http://www.medworm.com/index.php?rid=5058649&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc662v7256548up04%2F Content Type Journal ArticlePages 1-4DOI 10.1007/s00580-011-1280-xAuthors Mohammad Khosravi, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Azadi Avenue- Qareeb Street, 1419963111 Tehran, IranFariba Kavosh, Razi Vaccine and Serum Research Institute—Ahwaz Branch, Ahwaz, IranAhmad Taghavi-Moghadam, Razi Vaccine and Serum Research Institute—Ahwaz Branch, Ahwaz, IranShamsodin Ghaem-Maghami, Razi Vaccine and Serum Research Institute—Ahwaz Branch, Ahwaz, IranKhodadad Pirali-Kheirabadi, Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, IranPeyman Rahimi-Feyli, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranShahrokh Navid-Pour, Razi Vaccine and Serum... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058649 Thu, 21 Jul 2011 18:03:50 +0100 5058649 http://www.medworm.com/index.php?rid=5058650&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F315l201q7700561g%2F Abstract  Age-related cataract is a leading cause of blindness throughout the world. Regular exposure to sunlight increases the risk of cataract development. Ultraviolet light causes oxidative stress which induces DNA damage in the lens epithelium and the development of opacities. The existence of DNA repair in lens epithelial cells has been reported, and polymorphisms of DNA repair genes may affect repair efficiency. This work aims to study the frequency of polymorphisms of two DNA repair genes, xeroderma pigmentosum complementation group D (XPD) codon751 and X-ray cross-complementing group 1 (XRCC1) codon399, in cataract in the sunny weather of Egypt. Forty-two Egyptian patients with maturity onset cataract were compared to 41 control subjects using polymerase chain react... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058650 Thu, 21 Jul 2011 18:03:49 +0100 5058650 http://www.medworm.com/index.php?rid=5058652&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Faw747613g4767843%2F Abstract  The sialic acid complement of some selected organs of indigenous Nigerian Agama agama lizards and frogs, namely, heart, brain, liver, lungs and kidney, was determined using the thiobarbituric acid assay method and it was observed that the mean sialic acid concentration was highest in the kidney of female frogs (9.80 ± 1.20 mg/ml) followed by the liver of male frogs (9.0 ± 1.0 mg/ml) and least in the liver of female frogs (0.50 ± 0.080 mg/ml). Apart from establishing the baseline data on the sialic acid complement of the aforementioned organs in the above-mentioned species, this study also provides good information on the best organs for sialic acid isolation and preparation of sialic acid standard for research. Results from the present... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058652 Thu, 21 Jul 2011 18:03:48 +0100 5058652 http://www.medworm.com/index.php?rid=5058651&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj644m26421306q75%2F Abstract  Vascular calcifications are recognized as important risk factors for uremia-induced cardiovascular disease. Yet some patients with chronic kidney disease (CKD) do not develop calcification despite exposure to the same uremic conditions. Physiological inhibitors of calcification such as fetuin A, which is an extraosseous calcification inhibitor, and osteoprotegerin (OPG), which is a regulator of bone resorption, may prevent the development and progression of vascular calcification. The aim of this work is to study the serum levels of fetuin A and OPG to understand their role in vascular calcification in patients with chronic renal impairment. A total of 80 subjects (60 CKD patients and 20 age- and sex-matched healthy controls) were studied. Fetuin A and osteoproteg... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058651 Thu, 21 Jul 2011 18:03:48 +0100 5058651 http://www.medworm.com/index.php?rid=5058653&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm67u1p5x77332728%2F This study aims to evaluate a commercially available equine-optimized insulin assay and to evaluate the stability of equine insulin. In addition, serum insulin concentrations before and after feeding are also presented. Samples were taken before and after feeding from 40 healthy horses and from 15 equine patients visiting the University Equine Hospital. Insulin was analysed with the equine ELISA and with two human methods (one ELISA and one RIA). Precision was determined by repeated analysis of samples on one assay run and from one sample analysed on 15 different assays. Recovery from two dilution series and from an additional study was evaluated. Stability of equine insulin was evaluated in samples with and without haemolysis stored at 18–20°C, 6–8°C for 30 days and at �... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058653 Tue, 19 Jul 2011 23:46:25 +0100 5058653 http://www.medworm.com/index.php?rid=5058654&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F637624mwu551863t%2F Abstract  The purpose of this study was to determine the values of some metabolites, ions, and enzymes in maternal blood serum and fetal fluids in relation to pregnancy stage in singleton pregnant goats. Gravid uteri of goats were collected from local abattoirs. The allantoic and amniotic fluids as well as maternal blood samples were collected. Fetal age was determined according to crown–rump length by applying the age estimation formula that previously was presented for goat. The pregnancies were divided into five stages as: stage I (0–30 days), stage II (31–60 days), stage III (61–90 days), stage IV (91–120 days), and stage V (121 days to term). With the progress of pregnancy, the biochemical levels of fetal fluids and maternal serum chan... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5058654 Tue, 19 Jul 2011 06:41:17 +0100 5058654 http://www.medworm.com/index.php?rid=5043074&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa02t5u5367421rq1%2F This study was conducted to assess the effect of short-term exposure to RF waves generated by BTS antenna on the viability and motility of stored sperm in different parts of the epididymis. One hundred testes from slaughtered bulls were collected and used for this study. The testes were divided into two groups, test and sham-exposed, each group, according to time of exposure to RF (1 to 5 h), was divided into five subgroups, ten in each group. After a defined time, the motility (tail of the epididymis) and viability of sperms (in three parts of the epididymis) were evaluated in both groups. In the head of the epididymis, the reduction of sperm viability observed in the test group was 13% (after 3 h), 18% (after 4 h) and 21% (after 5 h) compared to the sham-exposed... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5043074 Fri, 15 Jul 2011 15:59:03 +0100 5043074 http://www.medworm.com/index.php?rid=5012685&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu0246r3371832844%2F Content Type Journal ArticlePages 1-1DOI 10.1007/s00580-011-1279-3Authors Hassan Morovvati, Department of Histology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, P.O. Box 61355–145, Ahvaz, IranYadollah Nikpour, Khoramshahr Marine Science and Technology University, P.O. Box 669, Khoramshar, IranHossein Zolgharnein, Khoramshahr Marine Science and Technology University, P.O. Box 669, Khoramshar, IranMohammadtaghi Ronagh, Khoramshahr Marine Science and Technology University, P.O. Box 669, Khoramshar, IranRahim Abdi, Khoramshahr Marine Science and Technology University, P.O. Box 669, Khoramshar, IranAram Roshan, Khoramshahr Marine Science and Technology University, P.O. Box 669, Khoramshar, Iran Journal Comparative Clinical PathologyOnline ISSN 1618-565XPrint... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5012685 Thu, 07 Jul 2011 05:50:43 +0100 5012685 http://www.medworm.com/index.php?rid=5012687&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc753472845377413%2F Abstract  Cladribine is a cytotoxic agent with high activity in hematological malignancies. It is usually administered intravenously. The study was carried out on 20 Egyptian hairy cell leukemia cases; 17 cases were de novo, and 3 cases were relapsed. Four (20%) were treated with IV cladribine, and 16 (80%) were treated with subcutaneous (SC) cladribine. An average duration of response was 36 months with no relapses in cases receiving IV cladribine vs. 25% relapse rate in cases receiving SC cladribine, with no significant difference between them. The subcutaneous administration of cladribine has a positive benefit/risk ratio in the treatment of patients with hairy cell leukemia. Content Type Journal ArticlePages 1-6DOI 10.1007/s00580-011-1277-5Authors Nehad M. Ta... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5012687 Wed, 06 Jul 2011 06:34:03 +0100 5012687 http://www.medworm.com/index.php?rid=5012686&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F450548649j653576%2F Abstract  Oxidative stress resulting from increased free radical generation plays an important role in carcinogenesis. The present study investigates the therapeutic efficacy of Semecarpus anacardium Linn nut milk extract (SA) against lipid peroxidation (LPO) and antioxidant status in spleen and thymus of experimental animals. Adult female albino rats of Sprague–Dawley strain weighing 170–190 g were used for the study and were divided into four groups. Group I control animals received standard pellet diet and water ad libitum. Group II rats were induced with 7,12-dimethyl benz[a]anthracene (DMBA) (25 mg in 1 ml olive oil) by gastric incubation, whereas another set of DMBA-induced rats were treated with SA (200 mg/kg body weight/day) in olive oil orall... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=5012686 Wed, 06 Jul 2011 06:34:03 +0100 5012686 http://www.medworm.com/index.php?rid=4994936&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu210807723g68252%2F Abstract  Neonatal diarrhea in calves causes intestinal inflammation that can lead to an increase in the concentration of some inflammatory mediators like nitric oxide in serum. In the presence of some molecules in biological fluids, nitric oxide sustains a series of reactions and produces nitrate and nitrite. Consequently, this paper has been done to determine if the serum levels of nitrite and nitrate can be a precise yardstick to evaluate the health condition of calves. Sixty calves in three groups (20 heads healthy as control group, 20 heads with chronic diarrhea, and 20 heads with acute diarrhea) were considered, and blood samples were collected to determine the concentration of nitrite and nitrate in serum by reducing by cadmium and evaluation of photo-absorbance by s... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=4994936 Thu, 30 Jun 2011 09:38:44 +0100 4994936 http://www.medworm.com/index.php?rid=4988048&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx32h66m331736v42%2F Abstract  In autumn 2009, 87 blood samples (28 male and 59 female) were taken, via jugular vein puncture using a heparinised venoject, from Makuii sheep in a preserved herd. The samples were divided into four age groups. Blood samples were transferred to a laboratory on ice, and the serum samples were separated from whole blood by centrifugation. Concentration of zinc and copper was measured in the serum by atomic absorption spectrophotometry. The mean ± SE of zinc and copper was 8.34 ± 1.07 and 12.32 ± 0.28 μmol/l, respectively. Zinc values were less than the normal expected range (P < 0.05), but the copper value was normal in serum samples. It is possible that there is zinc deficiency in the herd in this part of Iran. Content Type Journal A... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=4988048 Tue, 28 Jun 2011 15:48:40 +0100 4988048 http://www.medworm.com/index.php?rid=4972026&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fnwwu414577852672%2F Abstract  Aqueous extract of Abrus precatorius leaf was claimed to have haemopoeitic effect. Forty-two mice that weighed 25.76 ± 2.39 g were administered 0.0, 12.5, 25.0, 50.0, 100.0 and 200.0 mg/kg body weight of aqueous extract of A. precatorius leaf for a period of 3 weeks. Pretreatment blood sample (0.3 mL) was collected from tail vein of each mouse on day 1 and thereafter on days 7, 14 and 21 of the experimentation for determination of packed cell volume and plasma electrolytes. However, total blood volume was determined by multiplying plasma volume by 100/100-haematocrit, and red cell volume was determined by subtracting plasma volume from total blood volume. The results showed significant (P < 0.05) increased weight gain in gro... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=4972026 Fri, 24 Jun 2011 16:14:55 +0100 4972026 http://www.medworm.com/index.php?rid=4963471&cid=s_33457_32_f&fid=33457&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr50721522nl04q42%2F This study was conducted to evaluate the cytological effects of maternal diabetes in the central nervous system (CNS). Sixteen adult female rats were divided in two groups and diabetes was induced in one group using alloxan. Both groups became pregnant by natural mating. At day 7 after birth, the cerebrum, cerebellum and spinal cord were collected from offspring of all rats. The weight of the neonates and their blood glucose were measured. Various cellular parameters were determined using transmission electron microscopy. Results revealed changes in the neurons of the grey matter of the cerebrum, cerebellum and spinal cord in the offspring of diabetic mothers (ODM) compared to controls. Mitochondrial abnormalities were also detected; mitochondrial cristae were destroyed and the ... Comparative Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=4963471 Wed, 22 Jun 2011 15:57:15 +0100 4963471