MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cryobiology' source. Mon, 06 Feb 2012 14:26:06 +0100 http://www.medworm.com/index.php?rid=5638577&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22280955%26dopt%3DAbstract This study found that this cryopreservation protocol does not affect the biological properties of hAMSC. DISCUSSION: This shows that this protocol is a viable system for banking hAMSC, with the associated advantages that has a low cost in terms of expense, time and personnel involved and is easy to implement. PMID: 22280955 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5638577 Fri, 20 Jan 2012 05:00:00 +0100 5638577 http://www.medworm.com/index.php?rid=5638579&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274740%26dopt%3DAbstract CONCLUSIONS: Multiple-CPA solutions are less cytotoxic than single-CPA solutions of the same total concentration. PG was the most toxic CPA when used in combinations. The highest chondrocyte survival rates were obtained with the 6.0M DMSO-EG-Gy-Fm combination solution. PMID: 22274740 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5638579 Wed, 18 Jan 2012 05:00:00 +0100 5638579 http://www.medworm.com/index.php?rid=5638578&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22280954%26dopt%3DAbstract CONCLUSION: Using the methods described here, long-term (23-25years) cryopreserved human BMCs can be successfully cultivated to obtain MSCs that have good differentiation capabilities. PMID: 22280954 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5638578 Wed, 18 Jan 2012 05:00:00 +0100 5638578 http://www.medworm.com/index.php?rid=5638576&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22280956%26dopt%3DAbstract In conclusion immature goat COCs vitrified using CT and HS showed that viability, maturation rates and expression of genes coding for oocyte secreted factors and apoptosis were similar to non-vitrified controls. PMID: 22280956 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5638576 Wed, 18 Jan 2012 05:00:00 +0100 5638576 http://www.medworm.com/index.php?rid=5638580&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22266474%26dopt%3DAbstract This study characterized betaine formulations, with and without sucrose, and tested these with the model lactic acid bacteria Lactobacillus coryniformis Si3. Betaine alone did not act as a lyo-protectant and thus betaine import prior to freeze-drying should be avoided. Differences in protective agents were analyzed by calorimetry, which proved to be a suitable tool for evaluating the characteristics of the freeze-dried end products. PMID: 22266474 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5638580 Sun, 15 Jan 2012 05:00:00 +0100 5638580 http://www.medworm.com/index.php?rid=5620638&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248796%26dopt%3DAbstract In this study, we have used previously published data to determine a toxicity cost function, a quantity that represents the cumulative damage caused by toxicity. We then used this cost function to define and numerically solve the optimal control problem for CPA equilibration, using human oocytes as representative cell type with high clinical relevance. The resulting toxicity-optimal procedures are predicted to yield significantly less toxicity than conventional stepwise procedures. In particular, our results show that toxicity is minimized during CPA addition by inducing the cell to swell to its maximum tolerable volume and then loading it with CPA while in the swollen state. This counterintuitive result is considerably different from the conventional stepwise strategy, which involves expo... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5620638 Tue, 10 Jan 2012 05:00:00 +0100 5620638 http://www.medworm.com/index.php?rid=5577925&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227231%26dopt%3DAbstract Authors: Terrell KA, Wildt DE, Anthony NM, Bavister BD, Leibo SP, Penfold LM, Marker LL, Crosier AE Abstract Felid spermatozoa are sensitive to cryopreservation-induced damage, but functional losses can be mitigated by post-thaw swim-up or density gradient processing methods that selectively recover motile or structurally-normal spermatozoa, respectively. Despite the importance of sperm energy production to achieving fertilization, there is little knowledge about the influence of cryopreservation or post-thaw processing on felid sperm metabolism. We conducted a comparative study of domestic cat and cheetah sperm metabolism after cryopreservation and post-thaw processing. We hypothesized that freezing/thawing impairs sperm metabolism and that swim-up, but not density gradient centri... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5577925 Thu, 29 Dec 2011 05:00:00 +0100 5577925 http://www.medworm.com/index.php?rid=5577926&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22222678%26dopt%3DAbstract Authors: Mirabet V, Alvarez M, Solves P, Ocete D, Gimeno C Abstract Cryopreservation is widely used for banking cells and tissues intended for transplantation. Liquid nitrogen provides a very stable ultra-low temperature environment. Thus, it is used for longterm storage. Unlike the exhaustive microbiological monitoring of the environmental conditions during tissue processing, storage is not usually considered as a critical point of potential contamination risk in professional standards for cell and tissue banking. We have analysed the presence of microbial agents inside our nitrogen tanks. We have mainly detected environmental and water-borne bacteria and fungi. In addition, we have studied the effect of liquid nitrogen exposure on virus detectability. Only differences for hepatit... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5577926 Wed, 28 Dec 2011 05:00:00 +0100 5577926 http://www.medworm.com/index.php?rid=5559845&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209823%26dopt%3DAbstract Authors: Felipe-Pérez YE, Valencia J, Juárez-Mosqueda ML, Pescador N, Roa-Espitia AL, Hernández-González EO Abstract The cryopreservation process has an important impact on sperm structure and physiology. The negative effects have been mainly observed on the plasma membrane, which is directly stabilized by the cytoskeleton. Since cytoskeleton proteins are osmosensitive and thermosensitive, the aim of this study was to evaluate the damage caused to the bull sperm cytoskeleton by cryopreservation (freezing-thawing). Fresh and frozen-thawed bull semen samples were exposed to a treatment with the neutral detergent Brij 36-T. Electron microscopy evidenced important damages at the sperm perinuclear theca after the protein extraction protocol; the perinuclear theca was partially solub... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5559845 Mon, 26 Dec 2011 05:00:00 +0100 5559845 http://www.medworm.com/index.php?rid=5559847&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22202670%26dopt%3DAbstract Authors: Benson JD Abstract The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the determination of the osmotically inactive volume and, specifically, an argument was made suggesting that this volume was incorrectly determined in the literature [4]. Here we show that this assertion is false. PMID: 22202670 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5559847 Tue, 20 Dec 2011 05:00:00 +0100 5559847 http://www.medworm.com/index.php?rid=5559846&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22202671%26dopt%3DAbstract Authors: Liang Y, Ning FY, Du WJ, Wang CS, Piao SH, An TZ Abstract To improve the vitrification of mouse oocytes using straws, we attempted to estimate the type and extent of injuries during vitrification with a vitrification solution EAFS10/10. Injuries in oocytes were assessed based on cellular viability, the integrity of the plasma membrane, the status of the meiotic spindle/chromosomes, and morphological appearance. For morphologically normal oocytes, the ability to be fertilized and to develop into blastocysts was examined. Morphological assessment revealed 15% of oocytes to be injured by intracellular ice formed during vitrification, and 10% by osmotic swelling during removal of the cryoprotectant. When assessed by the status of spindles/chromosomes, the most sensitive criter... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5559846 Mon, 19 Dec 2011 05:00:00 +0100 5559846 http://www.medworm.com/index.php?rid=5548773&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22197768%26dopt%3DAbstract Authors: John Morris G, Acton E, Murray BJ, Fonseca F Abstract The cellular damage that spermatozoa encounter at rapid rates of cooling has often been attributed to the formation of intracellular ice although no convincing evidence of intracellular ice formation has ever been obtained. We demonstrate that the high intracellular protein content together with the osmotic shrinkage associated with extracellular ice formation leads to intracellular vitrification of spermatozoa during cooling. At rapid rates of cooling the cell damage to spermatozoa is a result of an osmotic imbalance encountered during thawing, not intracellular ice formation. The osmotic imbalance occurs at rapid cooling rates due to a diffusion limited ice crystallisation in the extracellular fluid, i.e. the amount o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5548773 Wed, 14 Dec 2011 05:00:00 +0100 5548773 http://www.medworm.com/index.php?rid=5533213&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155480%26dopt%3DAbstract Authors: Chakraborty N, Menze MA, Elmoazzen H, Vu H, Yarmush ML, Hand SC, Toner M Abstract Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disaccharide trehalose. Trehalose is known to improve desiccation tolerance in mammalian cells when present on both sides of the cell membrane. Because trehalose is membrane impermeant the development of desiccation strategies involving this promising sugar is hindered. We explored the potential of using a high-capacity trehalose transporter (TRET1) from the African chironomid Polypedilum ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533213 Sat, 03 Dec 2011 05:00:00 +0100 5533213 http://www.medworm.com/index.php?rid=5533214&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22142903%26dopt%3DAbstract Authors: Lawson A, Mukherjee IN, Sambanis A Abstract Long-term storage of natural tissues or tissue-engineered constructs is critical to allow off-the-shelf availability. Vitrification is a method of cryopreservation that eliminates ice formation, as ice may be detrimental to the function of natural or bioartificial tissues. In order to achieve the vitreous state, high concentrations of CPAs must be added and later removed. The high concentrations may be deleterious to cells as the CPAs are cytotoxic and single-step addition or removal will result in excessive osmotic excursions and cell death. A previously described mathematical model accounting for the mass transfer of CPAs through the sample matrix and cell membrane was expanded to incorporate heat transfer and CPA cytotoxicity.... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533214 Mon, 28 Nov 2011 05:00:00 +0100 5533214 http://www.medworm.com/index.php?rid=5533215&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22138351%26dopt%3DAbstract Authors: Temmerman L, Vral A, Meire M, Verbeeck RM, Deschepper E, Dermaut LR, De Pauw GA Abstract The purpose of this in vivo study was to investigate revascularization and root growth after autotransplantation of cryopreserved immature teeth. Immature molar teeth were extracted in 4-week-old Wistar rats. In the test group, teeth were cryopreserved for 1week and transplanted subcutaneously to the abdomen. In the control group, teeth were transplanted subcutaneously immediately after extraction. Material was collected in test and control animals at intervals of 1, 2, 4 and 10weeks post-transplantation and histological and microradiographical examination was performed. Results showed that during the first weeks after transplantation, pulpal repair was similar in both groups although ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533215 Fri, 25 Nov 2011 05:00:00 +0100 5533215 http://www.medworm.com/index.php?rid=5533217&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22127305%26dopt%3DAbstract Authors: Fathi R, Valojerdi MR, Yazdi PE, Ebrahimi B, Alipour H, Hassani F Abstract This paper reports studies on the effects of re-vitrification by the CPS (Closed Pulled Straw) method on the development of 4-cell stage mouse embryos. The procedure involved culturing 2-cell mouse embryos in G-1 medium until the 4-cell stage followed by the division of the normal 4-cell stage embryos into a control group (non-vitrified) and two experimental subgroups (vitrified and re-vitrified). Embryos in the vitrified subgroup were cryopreserved by the CPS vitrification method. In the second experimental subgroup (re-vitrified), embryos that were already vitrified were warmed and cryopreserved again by the same method. There was no significant reduction in the rate of blastocyst formation after ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533217 Wed, 23 Nov 2011 05:00:00 +0100 5533217 http://www.medworm.com/index.php?rid=5533216&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22133891%26dopt%3DAbstract Authors: Ichikawa H, Nakata N, Abo Y, Shirasawa S, Yokoyama T, Yoshie S, Yue F, Tomotsune D, Sasaki K Abstract Cryopreservation is an essential technique in basic research and clinical applications of human embryonic stem (hES) cells. Cryopreserved hES cells are fragile and undergo post-thaw apoptosis. We performed gene pathway analysis on cryopreserved and thawed hES cells to examine the effect of Y-27632, a Rho-associated kinase (ROCK) inhibitor, on apoptosis and associated molecular events. Y-27632 was added to the cryopreservation solution and/or the post-thaw medium of two hES cell lines (KhES-1, KhES-3). Post-thaw apoptosis was recorded as a function of time using Giemsa staining and the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Apoptosis plateaued 1... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533216 Wed, 23 Nov 2011 05:00:00 +0100 5533216 http://www.medworm.com/index.php?rid=5533218&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22127304%26dopt%3DAbstract This study would provide theoretical support for the application of allogenic frozen-thawed bladder mucosa in the treatment of urethral stricture. Forty-two adult male New Zealand rabbits were used in this study. The immunogenicity was detected by mixed lymphocyte reaction using the allograft of bladder mucosa (fresh and frozen-thawed) and spleen lymphocytes. Twelve urethral stricture models were established in New Zealand rabbits for substitution urethroplasty using the allograft of bladder mucosa, which were divided into fresh and frozen-thawed group. Two weeks after operation, lymphocyte proliferation was detected in both blood and spleen of recipient rabbits. At the same time, immunohistochemical staining of urethral allograft was performed and the expression of CD3, CD4 and CD8 were o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5533218 Tue, 22 Nov 2011 05:00:00 +0100 5533218 http://www.medworm.com/index.php?rid=5455210&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119932%26dopt%3DAbstract This study evaluates the effects of SG decellularization and subsequent cryopreservation on the extracellular matrix integrity of allograft heart valves. Human aortic and pulmonary valves were trisected, with one-third of each either left fresh (no further processing after dissection), decellularized, or decellularized and cryopreserved. Two-photon laser scanning confocal microscopy was used to visualize collagen and elastin in leaflets and conduits. The optimized percent laser transmission (OPLT) required for full dynamic range imaging of each site was determined, and changes in OPLT were used to infer changes in collagen and elastin signal intensity. Collagen fiber crimp period and collagen and elastin fiber diameter were measured in leaflet tissue. Statistically significant differences ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5455210 Sat, 19 Nov 2011 05:00:00 +0100 5455210 http://www.medworm.com/index.php?rid=5379296&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22037613%26dopt%3DAbstract Authors: Magrini S, Scoppola A Abstract Pteridophytes spore banks are a promising ex situ conservation tool used to increase the chances of survival of ferns, in fact that large quantities of germplasm with high genetic variation can be conserved in a small space with low economic and technical costs. However, methods to maintain the viability of chlorophyllous spores during storage are less understood. The aim of this study was to investigate the influence of long term storage on the viability of Royal Fern spores, which were stored under different conditions derived from various combinations of temperature and degrees of hydration. Survival and germination tests were performed after 1 and 28months of storage. Our results showed the highest survival percentages for spores stored u... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5379296 Thu, 20 Oct 2011 04:00:00 +0100 5379296 http://www.medworm.com/index.php?rid=5379297&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22027383%26dopt%3DAbstract Authors: Barbaric I, Jones M, Buchner K, Baker D, Andrews PW, Moore HD Abstract Human embryonic stem cells (hESCs) can be maintained as undifferentiated cells in vitro and induced to differentiate into a variety of somatic cell types. Thus, hESCs provide a source of differentiated cell types that could be used to replace diseased cells of a tissue. The efficient cryopreservation of hESCs is important for establishing effective stem cell banks, however, conventional slow freezing methods usually lead to low rates of recovery after thawing cells and their replating in culture. We have established a method for recovering cryopreserved hESCs using pinacidil and compared it to a method that employs the ROCK inhibitor Y-27632. We show that pinacidil is similar to Y-27632 in promoting sur... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5379297 Mon, 17 Oct 2011 04:00:00 +0100 5379297 http://www.medworm.com/index.php?rid=5379299&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22020192%26dopt%3DAbstract In this study, we investigated the effect of vitrification and slow freezing on cartilage cell and tissue viability for biobanking. Firstly, primary adult cartilage cells (ACCs) and fetal cartilage cells (FCC) were cryopreserved by vitrification and slow freezing. Cells were vitrified after a two-step equilibration in a solution composed of ethylene glycol (EG), Ficoll and sucrose. For slow freezing three different cooling rates (0.5, 1 and 2°C/min) were tested in straws. Secondly, the tissues taken from articular cartilage were cryopreserved by vitrification and slow freezing (1°C/min). The results revealed no significant difference between the viability ratios, proliferative activity and GAG synthesis of cartilage cells which were cryopreserved by using vitrification or slow freezing m... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5379299 Wed, 12 Oct 2011 04:00:00 +0100 5379299 http://www.medworm.com/index.php?rid=5379298&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22020295%26dopt%3DAbstract Authors: Choi J, Bischof JC Abstract While studies on the freezing of cells in suspension have been carried out extensively, corresponding studies with cells in the attached state and in tissue or tissue-equivalents are less developed. As attachment is a hallmark of the tissue state it is important to understand its impact on biophysics and viability to better apply freezing towards tissue preservation. The current study reports on observed biophysical response changes observed during freezing human dermal fibroblasts in suspension, attached cell, and fibrin tissue-equivalent models. Specifically, intracellular ice formation is shown to increase and dehydration is inferred to increase from suspension to attached systems. Biophysical model parameters fit to these experimental observ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5379298 Mon, 10 Oct 2011 04:00:00 +0100 5379298 http://www.medworm.com/index.php?rid=5379300&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22005593%26dopt%3DAbstract In this study, we investigated the optimal temperature range for preserving dissociated cardiomyocytes for 72h while maintaining their normal growth and beating functions. Neonatal rat cardiomyocytes dissociated by collagenase and suspended in the culture medium were preserved at temperatures from -2 to 35°C for 72h. The cardiomyocytes preserved at temperatures below 20°C maintained the initial dispersed states, whereas they aggregated robustly at higher temperatures. The viability of the dispersed cells after preservation was more than 80%. After the preservation, the microscopic observations during the 7-days cultivation indicated that these dispersed cardiomyocytes grew normally to form a confluent monolayer, and beat spontaneously and regularly during culture, as did the fresh cells.... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5379300 Sat, 08 Oct 2011 04:00:00 +0100 5379300 http://www.medworm.com/index.php?rid=5325670&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21996161%26dopt%3DAbstract Authors: Megha KB, Banerjee S, Mohanan PV Abstract The feasibility of an indigenously developed ELISA method to determine cytokine response to wide spectrum of pyrogenic stimuli utilizing fresh human whole blood is limited by the availability of healthy donors. The possibility of using cryopreservation of pooled human blood for detection of cytokine response to lipopolysaccharide is explored in this study. The effect of cryopreservation on blood parameters, cellular morphology and cytokine response were compared with that of the pooled fresh blood and cryopreserved blood from single and multiple donors. In vitro pyrogenic stimulation with 0.5 and 5EU of LPS was monitored on fresh and cryopreserved pooled blood from single and multiple donors. The release of IL-1β was quantitated b... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325670 Sun, 02 Oct 2011 04:00:00 +0100 5325670 http://www.medworm.com/index.php?rid=5325674&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21985766%26dopt%3DAbstract In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. PMID: 21985766 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325674 Sat, 01 Oct 2011 04:00:00 +0100 5325674 http://www.medworm.com/index.php?rid=5325673&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21985767%26dopt%3DAbstract We examined the impact of bovine oocyte in vitro maturation (IVM) conditions on subsequent embryo yield, quality and cryosurvival. Cumulus-oocyte complexes (COCs) were sampled for cytological and gene expression analysis after IVM in TCM199 supplemented with 10% fetal bovine serum (FBS), 4mg/ml of fatty-acid-free bovine serum albumin (FAFBSA), 4mg/ml of polyvinylpyrrolidone (PVP), FAFBSA with epidermal growth factor (EGF, 100ng/ml) and insulin-like growth factor 1 (IGF-I, 100ng/ml) (FAFBSAGF), PVP with EGF and IGF-I (PVPGF) or PVP with single strength BME and MEM amino acids (PVPAA). The remaining COCs were fertilized. On day 7 (IVF=day 0) quality 1 blastocysts were vitrified or analyzed for glucose transporter 1 (Glut-1) expression levels. The remaining blastocysts (days 7-9) were evaluat... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325673 Sat, 01 Oct 2011 04:00:00 +0100 5325673 http://www.medworm.com/index.php?rid=5325672&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21985768%26dopt%3DAbstract CONCLUSIONS: Post-implant cryograft remodeling improved native vessels/cryografts coupling. Cryografts would have mechanical and geometrical advantages over ePTFE. Anastomotic cryograft remodeling differed from that expected only due to haemodynamic factors. The structural properties of the remodeled cryografts contribute to explain their functional characteristics. PMID: 21985768 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325672 Sat, 01 Oct 2011 04:00:00 +0100 5325672 http://www.medworm.com/index.php?rid=5325671&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21985769%26dopt%3DAbstract In conclusion, dimethylformamide could be used as an alternative cryoprotectant for goat semen freezing. However it was showed that no benefits were derived by using dimethylformamide to replace glycerol at an equal 6% concentration. PMID: 21985769 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325671 Sat, 01 Oct 2011 04:00:00 +0100 5325671 http://www.medworm.com/index.php?rid=5325676&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21982952%26dopt%3DAbstract CONCLUSION: Cryoplasty demonstrated superior rates of SMC apoptosis at 30min and 72h and was associated to relatively low arterial injury and inflammation scores. An immediate second PolarCath inflation did not achieve superior apoptosis. PMID: 21982952 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325676 Thu, 29 Sep 2011 04:00:00 +0100 5325676 http://www.medworm.com/index.php?rid=5325675&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21982953%26dopt%3DAbstract Authors: Huang KM, Peng M, Feng YQ, Huang H, Tu HJ, Luo J, Zhang L, Yuan XH, Wang LC Abstract Glioma, a type of brain tumor originating from glioma cells, varies widely in aggressiveness and causes serious symptoms, but the treatments are limited. Studies have shown that cryosurgery has multiple effects on tumor treatments, and administration of human tumor necrosis factor-alpha (rhTNF-α) arguments the anti-tumor effect of cryotherapy in breast and prostate cancers. To test the hypothesis that cryosurgery and rhTNF-α play synergistic effects against brain tumors, we established a brain glioma model on rat cortex regions following different treatments: the G1 group was sham-operated; the G2 group was treated with cryosurgery; the G3 group was treated with rhTNF-α; and G4 group re... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5325675 Thu, 29 Sep 2011 04:00:00 +0100 5325675 http://www.medworm.com/index.php?rid=5296237&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21963380%26dopt%3DAbstract This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatching and completely hatched. In vitro produced (IVP) blastocysts were derived from prepubertal goat oocytes by slicing of ovaries from slaughtered animals while adult goat oocytes were collected by the laparoscopic ovum pick up (LOPU) method. Blastocysts were vitrified/warmed using the cryotop technique. Survival rates were determined in terms of blastocoele re-expansion at 3 and 20h post-warming. For prepubertal goats, survival rates at 3h post-warming were significantly highe... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5296237 Thu, 22 Sep 2011 04:00:00 +0100 5296237 http://www.medworm.com/index.php?rid=5296236&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21963423%26dopt%3DAbstract Authors: Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H Abstract Insufficient bone union is the occasional complication of biomechanical reconstruction after malignant bone tumor resection using temperature treated tumor bearing bone; freezing, pasteurization, and autoclaving. Since bone morphogenetic protein (BMP) plays an important role in bone formation, we assessed the amount and activity of BMP preserved after several temperature treatments, including -196 and -73°C for 20min, 60 and 100°C for 30min, 60°C for 10h following -80°C for 12h as an allograft model, and 4°C as the control. The material extracted from the human femoral bone was treated, and the amount of BMP-7 was analyzed using an enzyme-link... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5296236 Mon, 19 Sep 2011 04:00:00 +0100 5296236 http://www.medworm.com/index.php?rid=5277090&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945817%26dopt%3DAbstract The objective of this experiment was to determine the optimal developmental stage to vitrify in vitro cultured porcine parthenogenetically activated (PA) embryos. Embryos were vitrified by Cryotop on Day 4, 5 or 6 after oocyte activation (Day 0), and immediately after warming they were either time-lapse monitored for 24h or analyzed by differential staining. After warming, the embryos had to be cultured for at least 8h before their survival rates were stabilized. Both the survival rate at 8h and the hatching rate at 24h of Day 4 embryos were significant higher than those vitrified on Day 5 or 6 (P<0.05), no matter if they were morulae or blastocysts. These results demonstrate that porcine PA embryos can survive successfully after vitrification/warming, that the optimal time for vitrific... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5277090 Fri, 16 Sep 2011 04:00:00 +0100 5277090 http://www.medworm.com/index.php?rid=5277089&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945818%26dopt%3DAbstract Authors: Başpınar N, Coyan K, Bucak MN Abstract The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2mM dithiothreitol and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed individually at 37°C for 20s in a water bath for evaluation. The addition of dithioerythritol at 0.5 and 2mM doses led to higher percentages of subjective motility (62.9±4.2% and 63.6±1.8%) compared to control (52.0±4.9%, P... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5277089 Fri, 16 Sep 2011 04:00:00 +0100 5277089 http://www.medworm.com/index.php?rid=5277088&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945819%26dopt%3DAbstract Authors: Zhou J, Zhang C, Liu J, Fan L, Yang L Abstract The current study aims to optimize the compositions of platelet activation-inhibitors for a loading solution of lyophilizing protectants and to establish a series of perfect pretreatment methods for platelet lyophilization. The optimal combination of six kinds of inhibitors and loading solutions of lyophilizing protectants, including prostaglandin E1 (PGE1), adenosine, l-arginine, phyticacid, bivalirudin, and cilostazol, was analyzed using the orthogonal experimental design. The values of the expression rates of p-selectin (CD62p) and platelet membrane glyeoprotein (PAC-1), as well as of platelet and mean platelet volume (MPV), were selected as indices of platelet activation. The values of CD62p and Pac-1 induced by thrombin w... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5277088 Fri, 16 Sep 2011 04:00:00 +0100 5277088 http://www.medworm.com/index.php?rid=5218470&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910982%26dopt%3DAbstract Authors: Beier AF, Schulz JC, Dörr D, Katsen-Globa A, Sachinidis A, Hescheler J, Zimmermann H Abstract Human embryonic stem cells (hESCs) are candidates for many applications in the areas of regenerative medicine, tissue engineering, basic scientific research as well as pharmacology and toxicology. However, use of hESCs is limited by their sensitivity to freezing and thawing procedures. Hence, this emerging science needs new, reliable preservation methods for the long-term storage of large quantities of functional hESCs remaining pluripotent after post-thawing and culturing. Here, we present a highly efficient, surface based vitrification method for the cryopreservation of large numbers of adherent hESC colonies, using modified cell culture substrates. This technique results in mu... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218470 Sat, 03 Sep 2011 04:00:00 +0100 5218470 http://www.medworm.com/index.php?rid=5218472&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21906586%26dopt%3DAbstract Authors: Kuwabara C, Kasuga J, Wang D, Fukushi Y, Arakawa K, Koyama T, Inada T, Fujikawa S Abstract Deep supercooling xylem parenchyma cells (XPCs) in Katsura tree contain flavonol glycosides with high supercooling-facilitating capability in solutions containing the ice nucleation bacterium (INB) Erwinia ananas, which is thought to have an important role in deep supercooling of XPCs. The present study, in order to further clarify the roles of these flavonol glycosides in deep supercooling of XPCs, the effects of these supercooling-facilitating (anti-ice nucleating) flavonol glycosides, kaempferol 3-O-β-d-glucopyranoside (K3Glc), kaempferol 7-O-β-d-glucopyranoside (K7Glc) and quercetin 3-O-β-d-glucopyranoside (Q3Glc), in buffered Milli-Q water (BMQW) containing different kinds of... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218472 Sat, 27 Aug 2011 04:00:00 +0100 5218472 http://www.medworm.com/index.php?rid=5218473&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21896271%26dopt%3DAbstract This report describes the feasibility of using vitrification for fish sperm. Vitrification can be used to preserve samples in the field and offers an alternative to conventional cryopreservation, although it has not been systematically studied for sperm of aquatic species. The overall goal of the project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of aquatic species germplasm. The objectives of the present study in channel catfish (Ictalurus punctatus) were to: (1) evaluate the acute toxicity of 5%, 10%, 20% and 30% methanol, N,N-dimethyl acetamide, dimethyl sulfoxide, 1,2-propanediol, and methyl glycol; (2) evaluate a range of devices commonly used for cryopreservation and vitrification of mammalian sperm; (3) compare vitrif... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218473 Fri, 26 Aug 2011 04:00:00 +0100 5218473 http://www.medworm.com/index.php?rid=5218471&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21906587%26dopt%3DAbstract This study presents the AFP from F. cylindrus set in a molecular and crystallographic frame. Differential protein expression after exposure of the diatoms to environmentally relevant conditions underlined the importance of certain AFP isoforms in response to cold. Analyses of the recombinant AFP showed freezing point depression comparable to the activity of other moderate AFPs and further enhanced by salt (up to 0.9°C in low salinity buffer, 2.5°C at high salinity). However, unlike other moderate AFPs, its fastest growth direction is perpendicular to the c-axis. The protein also caused strong inhibition of recrystallization at concentrations of 1.2 and 0.12μM at low and high salinity, respectively. Observations of crystal habit modifications and pitting activity suggested binding of AFP... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218471 Fri, 26 Aug 2011 04:00:00 +0100 5218471 http://www.medworm.com/index.php?rid=5218475&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21893053%26dopt%3DAbstract In conclusion, the study has shown that the cryopreservation process induced PTP in spermatozoa and their proportions were similar between portions of SRF. PMID: 21893053 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218475 Wed, 24 Aug 2011 04:00:00 +0100 5218475 http://www.medworm.com/index.php?rid=5218474&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21893054%26dopt%3DAbstract The objectives of this study were to examine the effects of the presence or absence of serum during the in vitro culturing period of domestic cat embryos on their developmental potential into blastocysts as well as their tolerance to cryopreservation using a slow-freezing method. In vitro-fertilized cat oocytes were incubated in a modified synthetic oviduct fluid (mSOF) containing 4mg/mL bovine serum albumin (BSA) throughout culturing (BSA group) or in mSOF containing 4mg/mL BSA for the first 3days followed by mSOF containing 5% fetal bovine serum (FBS group). The developmental potential of the embryos to the blastocyst and expanded blastocyst stages was evaluated 7days after in vitro fertilization. The blastocysts were frozen-thawed by the slow-freezing method and cultured for 3days to ex... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218474 Wed, 24 Aug 2011 04:00:00 +0100 5218474 http://www.medworm.com/index.php?rid=5218480&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21878325%26dopt%3DAbstract Authors: Zheng X, Cheng W, Wang X, Lei C Abstract Insects can increase their resistance to cold stress by prior exposure to non-lethal cold temperatures. Here, we investigated the supercooling capacity and survival of eggs, 3rd and 5th instar larvae, and pupae of Spodoptera exigua (Lepidoptera: Noctuidae) during CA, and responses to various pre-treatment protocols, including constant temperatures, thermoperiods, and RCH, RHH, RCH+RHH and RHH+RCH combined with thermoperiods. Only acclimated eggs demonstrated a significant decrease in SCP, from -20.7±0.3 to -22.9±0.3°C, among all experimental groups compared to non-acclimated stages. Survival increased by 17.5% for eggs, 40.0% and 13.3% for 3rd and 5th instar larvae, and by 20.0% for pupae after CA. Compared to controls, survival ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218480 Tue, 23 Aug 2011 04:00:00 +0100 5218480 http://www.medworm.com/index.php?rid=5218479&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884688%26dopt%3DAbstract This study revealed a positive effect of the M2 thawing methodology on sperm parameters. The percentage of spermatozoa with fast-linear movement is increased (M1: 17.26% vs. M2: 28.05%, p<0.01), with higher viability (M1: 37.81% vs. M2: 40.15%, p<0.01) and less acrosome damage (M1: 40.44% vs. M2: 35.45%, p=0.02). We also detected an increase in the percentage of viable spermatozoa with low membrane lipid disorder (M1: 31.36% vs. M2: 33.17%, p=0.03) and a reduction in chromatin condensation (44.62 vs. 46.62 arbitrary units, p=0.02). Further studies will be necessary to evaluate the possible clinical applications. PMID: 21884688 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218479 Tue, 23 Aug 2011 04:00:00 +0100 5218479 http://www.medworm.com/index.php?rid=5218478&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884689%26dopt%3DAbstract Authors: Kubota N Abstract Thermal hysteresis (TH) readings depend on the cooling rate, annealing time and the concentration of the biological antifreeze (AF) (i.e., antifreeze protein or antifreeze glycoprotein). Such time- and concentration-dependent TH readings are not true (or absolute) values. The true TH should be independent of time and AF concentration, and it should be a unique value for a given AF. Only the true TH can be used to assess the activity of an AF. A mathematical model is proposed to explain the time- and concentration-dependent behavior of AFs. The model assumes a reversible Langmuir adsorption mechanism for the AF molecules and the Kelvin effect to be applicable. A TH equation that correlates the TH reading with the cooling rate, annealing time and AF concent... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218478 Tue, 23 Aug 2011 04:00:00 +0100 5218478 http://www.medworm.com/index.php?rid=5218477&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884690%26dopt%3DAbstract Authors: Niknejad H, Deihim T, Solati-Hashjin M, Peirovi H Abstract Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibility of using the AM after different preservation methods to serve as substrates for endothelial cell expansion ex vivo. The effects of cryopreservation and lyophilization were evaluated on mechanical and histological characteristics of the AM, and the results were compared with the fresh AM. The ECM components of the basement membrane were well conserved in all groups. Although lyophilization resulted i... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218477 Tue, 23 Aug 2011 04:00:00 +0100 5218477 http://www.medworm.com/index.php?rid=5218476&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884691%26dopt%3DAbstract In this study we report the heterologous expression of two antifreeze protein isoforms from F. cylindrus in Escherichia coli. Refolding from inclusion bodies produced proteins functionally active with respect to crystal deformation, recrystallization inhibition and thermal hysteresis. We observed a reduction of activity in the presence of the pelB leader peptide in comparison with the GS-linked SUMO-tag. Activity was positively correlated to protein concentration and buffer salinity. Thermal hysteresis and crystal deformation habit suggest the affiliation of the proteins to the hyperactive group of AFPs. One isoform, carrying a signal peptide for secretion, produced a thermal hysteresis up to 1.53°C±0.53°C and ice crystals of hexagonal bipyramidal shape. The second isoform, which has a ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5218476 Tue, 23 Aug 2011 04:00:00 +0100 5218476 http://www.medworm.com/index.php?rid=5175011&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21856295%26dopt%3DAbstract Authors: Vitiello V, Carlino PA, Del Prete F, Langellotti AL, Sansone G Abstract The aim of this work was to evaluate the effects of temperature, cryoprotectant agents and freezing curves on sperm motility of Ostrea edulis. All phases of cryopreservation were studied (evaluation of semen motility pattern, choice of cryoprotectants and freezing rates) to restore after thawing the motility characteristics distinctive of fresh semen. To assess the temperature effects on sperm motility, semen was activated using four different temperatures (25, 18, 10 and 3°C). Sperm aliquots were maintained inactive at these temperatures for 1 and 3h, then activated with FSW at same temperature of conservation. Sperm was activated and incubated to 3°C with dimethylsulfoxide (M2SO), ethylene glycol (... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5175011 Wed, 10 Aug 2011 23:00:00 +0100 5175011 http://www.medworm.com/index.php?rid=5141209&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21820425%26dopt%3DAbstract This study focused on the effect of chilling and subsequent warming on gene expression of sox2, sox3 and sox19a which play vital roles in the development of zebrafish embryos. A quantitative RT-PCR approach was used to investigate gene expression following chilling at 0°C for up to 180min. The effect on gene expression was also studied during a 180min warming period after chilling for 30 or 60min. There were significant decreases in sox2 (up to 4-fold) and sox3 (up to 3-fold) expressions following chilling. Significant increases in gene expressions of sox2 (up to 2-fold), sox3 (up to 33-fold) and sox19a (up to 25-fold) were observed during warming in the embryos that had been chilled for 30min. Similarly, significant increases were observed in sox2 (up to 3-fold) and sox3 (up to 2-fold) d... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5141209 Wed, 27 Jul 2011 23:00:00 +0100 5141209 http://www.medworm.com/index.php?rid=5141210&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21819976%26dopt%3DAbstract Authors: Qu T, Liu R, Wang W, An L, Chen T, Liu G, Zhao Z Abstract Pectin methylesterases (PMEs) are important cell wall enzymes that may play important roles in plant chilling/freezing tolerance. We investigated the possible roles of brassinosteroids (BRs) in regulation of PMEs under chilling stress. Chilling stress or 24-epibrassinolide (eBL) treatments induced significant increases in PME activity in wild type (Col-0) seedlings of Arabidopsis. The chilling-stress-induced increases in PME activity were also found in bzr1-D mutant, a BZR1 stabilized mutant with a constitutively active BR signaling pathway, but not in bri1-116, a BR insensitive null allele of the BR receptor BRI1. The results suggest that the regulation of PME activity in Arabidopsis under chilling stress depends o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5141210 Tue, 26 Jul 2011 23:00:00 +0100 5141210 http://www.medworm.com/index.php?rid=5097620&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21810414%26dopt%3DAbstract Authors: Diekmann U, Spindler R, Wolkers WF, Glasmacher B, Müller T Stem cell research is a highly promising and rapidly progressing field inside regenerative medicine. Embryonic stem cells (ESCs), reprogrammed "induced pluripotent" cells (iPS), or lately protein induced pluripotent cells (piPS) share one inevitable factor: mouse embryonic feeder cells (MEFs), which are commonly used for ESC long term culture procedures and colony regeneration. These MEFs originate from different mouse strains, are inactivated by different methods and are differently cryopreserved. Incomprehensibly, there are to date no established quality control parameters for MEFs to insure consistency of ESC experiments and culture. Hence, in this work, we developed a bench-top quality control for embryonic feeder... Cryobiology journals http://www.medworm.com/rss/comments.php?id=5097620 Sat, 23 Jul 2011 23:00:00 +0100 5097620 http://www.medworm.com/index.php?rid=4956243&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679702%26dopt%3DAbstract The objective of this study was to examine the effects of cholesterol loaded cyclodextrins (CLCs) on the quality of stallion epididymal sperm during cryopreservation. In experiment I, sperm were treated with different concentrations of CLCs: (1) 0mg (control), (2) 1.5mg, (3) 3mg, and (4) 6mg per 120×10(6) sperm. The sperm viability and amount of cholesterol were determined at 15, 30 and 45min after CLC treatment using viability markers (Ethidium homodimer-1 and Calcein AM) and gas chromatography, respectively. In experiment II, CLC treated sperm (1.5mg CLC per 120×10(6) sperm) were fixed and stained with filipin to examine the cholesterol distribution. In experiment III, sperm were treated with CLCs at concentrations of 1.5, 3.0, 6.0mg per 120×10(6) sperm for 15min, then equilibrated wi... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4956243 Sun, 12 Jun 2011 23:00:00 +0100 4956243 http://www.medworm.com/index.php?rid=4956242&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21684270%26dopt%3DAbstract Authors: Liu G, Ye X, Zhu Y, Li Y, Sun J, Cui L, Cao Y The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was to determine the effect of cryopreservation on the osteogenic differentiation of green fluorescent protein (GFP)-marked UCB-MSCs in vitro. MSCs were isolated from full-term human UCB, expanded, transfected with the GFP gene, and then cryopreserved in liquid nitrogen for 4weeks. After thawing, cell surface antigen markers and osteogenic potential were analyzed, and the luminescence of these cells was observed by fluoresc... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4956242 Sun, 12 Jun 2011 23:00:00 +0100 4956242 http://www.medworm.com/index.php?rid=4907303&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21645501%26dopt%3DAbstract CONCLUSION: Efficiency of HMP might be increased by stimulating livers with dopamine during ex vivo preservation, limiting vascular side effects and improving functional recovery upon early reperfusion. PMID: 21645501 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4907303 Sun, 29 May 2011 23:00:00 +0100 4907303 http://www.medworm.com/index.php?rid=4907304&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621529%26dopt%3DAbstract In this study, we developed a vitrification solution comprising ethylene glycol (EG) and sucrose as well as carboxylated ε-poly-l-lysine (PLL); this solution inhibited devitrification. Human iPS cells were vitrified in 200-μL vitrification solutions comprised 6.5M EG, 0.75M sucrose and 0 or 10%w/v carboxylated PLL with 65mol% of the amino groups converted to carboxyl groups [PLL (0.65)] in a cryovial by directly immersing in liquid nitrogen. After warming, attached colony and recovery rates of human iPS cells vitrified by adding PLL (0.65) were significantly higher than those for cells without PLL (0.65) and vitrification solution (DAP213: 2M dimethyl sulfoxide, 1M acetamide and 3M propylene glycol). Furthermore, even after warming at room temperature, attached colony and recovery rates ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4907304 Thu, 19 May 2011 23:00:00 +0100 4907304 http://www.medworm.com/index.php?rid=4907306&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21619875%26dopt%3DAbstract This study was undertaken in order to study the effect of freeze-drying in the structure of BP. To perform this study BP samples were freeze-dried in two different types of freeze-dryers available in our laboratory: a laboratory freeze-dryer, in which it was not possible to control parameters and a pilot freeze-dryer, wherein all parameters during freezing and drying were controlled. After freeze-drying processes, samples were analyzed by SEM, Raman spectroscopy, tensile strength, water uptake tests and TEM. In summary, it has been demonstrated that damages occur in collagen fibers by the loss of bulk water of collagen structure implicating in a drastic decreasing of BP mechanical properties due to its structural alterations. Moreover, it was proven that the collagen fibrils suffered break... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4907306 Mon, 16 May 2011 23:00:00 +0100 4907306 http://www.medworm.com/index.php?rid=4907305&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21620818%26dopt%3DAbstract Authors: Freimark D, Sehl C, Weber C, Hudel K, Czermak P, Hofmann N, Spindler R, Glasmacher B Human mesenchymal stem cells (hMSCs) have great potential for clinical therapy and regenerative medicine. One major challenge concerning their application is the development of an efficient cryopreservation protocol since current methods result in a poor viability and high differentiation rates. A high survival rate of cryopreserved cells requires an optimal cooling rate and the presence of cryoprotective agents (CPA) in sufficient concentrations. The most widely used CPA, dimethylsulfoxide (Me(2)SO), is toxic at high concentrations at temperatures >4°C and has harmful effects on the biological functionality of stem cell as well as on treated patients. Thus, this study investigates differe... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4907305 Mon, 16 May 2011 23:00:00 +0100 4907305 http://www.medworm.com/index.php?rid=4804257&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21549108%26dopt%3DAbstract Authors: Ding F, Lall SP, Li J, Lei J, Rommens M, Milley JE Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks' balanced salt solution (HBSS) and 0.1M KHCO(3) with 0.125M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6mL volumes. A cryopreservation volume of 4mL and a low dilution ratio (1:1) were examined separately. Based on m... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804257 Wed, 27 Apr 2011 23:00:00 +0100 4804257 http://www.medworm.com/index.php?rid=4804256&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21549109%26dopt%3DAbstract Authors: Corwin WL, Baust JM, Baust JG, Van Buskirk RG Human corneal endothelial cells (HCEC) have become increasingly important for a range of eye disease treatment therapies. Accordingly, a more detailed understanding of the processing and preservation associated stresses experienced by corneal cells might contribute to improved therapeutic outcomes. To this end, the unfolded protein response (UPR) pathway was investigated as a potential mediator of corneal cell death in response to hypothermic storage. Once preservation-induced failure had begun in HCECs stored at 4°C, it was noted that necrosis accounted for the majority of cell death but with significant apoptotic involvement, peaking at several hours post-storage (4-8h). Western blot analysis demonstrated changes associated with... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804256 Wed, 27 Apr 2011 23:00:00 +0100 4804256 http://www.medworm.com/index.php?rid=4804260&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21530502%26dopt%3DAbstract Authors: Lynch AL, Slater NK Significant interest exists in the application of trehalose, which has low permeability to the phospholipid bilayer, as a non-toxic intracellular cryopreservative for mammalian cells. Introduction of between 8±3mM and 266±22mM trehalose into human erythrocytes using the membrane permeabilizing polymer PP-50 allowed investigation of the relationship between intracellular trehalose concentration, pre-freeze cell volume, and cryosurvival. Cellular cryosurvival increased approximately linearly with pre-freeze cell volume up to the normal volume of fresh cells; diminished cell survival correlated with subnormal pre-freeze cell volume in some cases even at >100mM intracellular trehalose concentration. Uptake of >200mM trehalose in cells with near-normal c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804260 Wed, 20 Apr 2011 23:00:00 +0100 4804260 http://www.medworm.com/index.php?rid=4804259&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21536022%26dopt%3DAbstract Authors: Yang G, Zhang A, Xu LX Direct cell injury in cryosurgery is highly related to intracellular ice formation (IIF) during tissue freezing and thawing. Mechanistic understanding of IIF in tumor cells is critical to the development of tumor cryo-ablation protocol. In aid of a high speed CMOS camera system, the events of IIF in MCF-7 cells have been studied using cryomicroscopy. Images of 'darkening' type IIF and recrystallization are compared between cells frozen with and without ice seeding. It is found that ice seeding has significant impact on the occurrence and growth of intracellular ice. Without ice seeding, IIF is observed to occur over a very small range of temperature (∼1°C). The crystal dendrites are indistinguishable, which is independent of the cooling rate. Ice crys... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804259 Wed, 20 Apr 2011 23:00:00 +0100 4804259 http://www.medworm.com/index.php?rid=4804258&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21540134%26dopt%3DAbstract Authors: Sansinena M, Santos MV, Zaritzky N, Chirife J Oocyte cryopreservation is of key importance in the preservation and propagation of germplasm. Interest in oocyte cryopreservation has increased in recent years due to the application of assisted reproductive technologies in farm animals such as in vitro fertilization, nuclear transfer and the need for the establishment of ova/gene banks worldwide. However, the cryopreservation of the female gamete has been met with limited success mainly due to its small surface-area:volume ratio. In the past decade, several vitrification devices such as open pulled straws (OPS), fine and ultra fine pipette tips, nylon loops and polyethylene films have been introduced in order to manipulate minimal volumes and achieve high cooling rates. However, ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804258 Wed, 20 Apr 2011 23:00:00 +0100 4804258 http://www.medworm.com/index.php?rid=4804262&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21521636%26dopt%3DAbstract Authors: Krajňáková J, Sutela S, Aronen T, Gömöry D, Vianello A, Häggman H In coniferous species, including Greek fir (Abies cephalonica Loud), the involvement of somatic embryo plants in breeding and reforestation programs is dependent on the success of long-term cryostorage of embryogenic cultures during clonal field testing. In the present study on Greek fir, we assayed the recovery, morphological characteristics and genetic fidelity of embryogenic cell lines 6 and 8 during proliferation and maturation after long-term cryostorage. Our results indicate successful recovery of both cell lines after 6years in cryostorage. In the maturation phase, both cell lines were capable of producing somatic embryos although some differences were detected among experiments. However, these chan... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804262 Sat, 16 Apr 2011 23:00:00 +0100 4804262 http://www.medworm.com/index.php?rid=4804261&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21524646%26dopt%3DAbstract In this study, we present a cryopreservation technique based on low, non-toxic concentrations of cryoprotectants (≈2-4M) combined with a rapid cooling rate in the liquid nitrogen phase (-196°C). Protocols for successfully cryopreserving the plant parasitic nematodes Globodera tabacum tabacum, Heterodera schachtii and Meloidogyne incognita were developed, as demonstrated by the high survival rates and reproducibility of cyst and root-knot nematode species post-cryostorage. This approach for effective cryopreservation of viable plant-parasitic nematodes was developed by inducing an "apparent vitrification" by rapid cooling of the microscopic samples in less than 2M of cryoprotectant. The extremely thin structure (15-20μm width, 350-400μm length) of these nematodes, in combination with a... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804261 Sat, 16 Apr 2011 23:00:00 +0100 4804261 http://www.medworm.com/index.php?rid=4804264&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21513706%26dopt%3DAbstract Authors: Seita Y, Fujiwara K, Takizawa A, Furukawa K, Inomata T, Ito J, Kashiwazaki N For preservation of rat spermatozoa, the general-purpose method requires that the male be sacrificed for collection of spermatozoa from the epididymides. However, it would be highly useful if the ejaculated spermatozoa could be successfully cryopreserved and the frozen-thawed spermatozoa used for in vitro fertilization, since this would allow the genetically valuable rats to be maintained alive rather than sacrificed. The aim of the present study was to clarify whether ejaculated rat spermatozoa could be successfully cryopreserved and fertilized in vitro. The motility and viability of frozen-thawed ejaculated spermatozoa were similar to those of frozen-thawed epididymal spermatozoa (around 10%). The p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804264 Mon, 11 Apr 2011 23:00:00 +0100 4804264 http://www.medworm.com/index.php?rid=4804263&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21513707%26dopt%3DAbstract Authors: Coyan K, Başpınar N, Bucak MN, Akalın PP The aim of the current study was to evaluate the effects of cysteine and ergothioneine on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities. Semen samples from 5 mature Merino rams were used in the study. Semen samples, which were diluted with a Tris-based extender containing l-Cysteine and l-(+)-Ergothioneine and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed individually at 37°C for 20s in a water bath for evaluation. Ergothioneine at doses of 2 and 4mM increased percentages of subjective motility, VSL and VCL, compared to controls following the freeze-thawing (P<0.001). Ergothioneine at three different doses led to higher rates of p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4804263 Mon, 11 Apr 2011 23:00:00 +0100 4804263 http://www.medworm.com/index.php?rid=4697497&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21466797%26dopt%3DAbstract Authors: Kawase T, Kogami H, Nagata M, Uematsu K, Okuda K, Burns DM, Yoshie H Cultured human periosteal sheets constitute a promising grafting material for periodontal tissue regenerative therapy. However, preparation of these sheets usually requires six weeks or longer, and this lengthy commitment and delay limits both clinical applicability and availability. The aim of this study is to develop an efficient, practical, cost-effective cryopreservation method for periosteal tissue segments (PTSs). Human PTSs were aseptically excised from alveolar bone and pre-cultured in Medium 199 + 10% fetal bovine serum (FBS) for the indicated number of days before they were slowly frozen down to -75(o)C in a commercial freezing vessel using medium containing 10% dimethyl sulfoxide (Me(2)SO) and vari... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4697497 Fri, 01 Apr 2011 23:00:00 +0100 4697497 http://www.medworm.com/index.php?rid=4697498&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21463613%26dopt%3DAbstract Authors: Fadda S, Briesen H, Cincotti A Typical mathematical modelling of cryopreservation of cell suspensions assumes a thermodynamic equilibrium between the ice and liquid water in the extracellular solution. This work investigates the validity of this assumption by introducing a population balance approach for dynamic extracellular ice formation (EIF) in the absence of any cryo-protectant agent (CPA). The population balance model reflects nucleation and diffusion-limited growth in the suspending solution whose driving forces are evaluated in the relevant phase diagram - This population balance description of the extracellular compartment has been coupled to a model recently proposed in the literature [Fadda et al., AIChE Journal, 56, 2173-2185, (2010)], which is capable of quantitat... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4697498 Thu, 31 Mar 2011 23:00:00 +0100 4697498 http://www.medworm.com/index.php?rid=4697500&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457707%26dopt%3DAbstract Authors: Lauersen KJ, Brown A, Middleton A, Davies PL, Walker VK Antifreeze proteins (AFP) are an evolutionarily diverse class of stress response products best known in certain metazoans that adopt a freeze-avoidance survival strategy. The perennial ryegrass, Lolium perenne (Lp), cannot avoid winter temperatures below the crystallization point and is thought to use its LpAFP in a freeze-tolerant strategy. In order to examine properties of LpAFP in relation to L. perenne's life history, cDNA cloning, recombinant protein characterization, ice-binding activities, gene copy number, and expression responses to low temperature were examined. Transcripts, encoded by only a few gene copies, appeared to increase in abundance after diploid plants were transferred to 4°C for 1-2days, and in para... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4697500 Tue, 29 Mar 2011 23:00:00 +0100 4697500 http://www.medworm.com/index.php?rid=4697499&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21458437%26dopt%3DAbstract Authors: Weng L, Li W, Zuo J Quantitative analyses of the bound water content in the alcohol aqueous solution and its osmotic behavior should be cryobiologically significant. This paper has presented two applications of the thermogram of the alcohol/water system recorded by differential scanning calorimeter (DSC). Both applications are: 1. generating the quantitative relationship between the bound water content and the solution composition; 2. calculating the osmotic virial coefficients for alcohols. Five alcohols including methanol, ethanol, ethylene glycol, propylene glycol and glycerol are investigated. In the present study, partial binary phase diagrams of these five alcohol solutions are determined in the first place. The bound water contents in these solutions are quantitatively ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4697499 Tue, 29 Mar 2011 23:00:00 +0100 4697499 http://www.medworm.com/index.php?rid=4638147&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397593%26dopt%3DAbstract Authors: Abedini S, Kaku M, Kawata T, Koseki H, Kojima S, Sumi H, Motokawa M, Fujita T, Ohtani J, Ohwada N, Tanne K The purpose of this study was to evaluate the effects of long-term cryopreservation on the isolated human periodontal ligament cells (PDL) and pulp tissues. In the first part of study, 10 freshly extracted teeth were selected and divided into two groups. In the cryopreserved group, the teeth were frozen for 5years using a programmed freezer combined with a magnetic field, known as Cells Alive System "CAS". As for the control group, freshly extracted teeth were used. In each group, extracted PDL tissues were cultured and gene expression and protein concentration of collagen type I, alkaline-phosphatase (ALP) and vascular endothelial growth factor (VEGF) was compared betwee... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4638147 Thu, 17 Mar 2011 00:00:00 +0100 4638147 http://www.medworm.com/index.php?rid=4638146&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21420397%26dopt%3DAbstract Authors: Dang NX, Hincha DK Hydrophilins are a group of proteins that are present in all organisms and that have been defined as being highly hydrophilic and rich in glycine. They are assumed to play important roles in cellular dehydration tolerance. There are twelve genes in the yeast Saccharomyces cerevisiae that encode hydrophilins and most of these genes are stress responsive. However, the functional role of yeast hydrophilins, especially in desiccation and freezing tolerance, is largely unknown. Here, we selected six candidate hydrophilins for further analysis. All six proteins were predicted to be intrinsically disordered, i.e. to have no stable structure in solution. The contribution of these proteins to the desiccation and freezing tolerance of yeast was investigated in the res... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4638146 Thu, 17 Mar 2011 00:00:00 +0100 4638146 http://www.medworm.com/index.php?rid=4578995&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21376028%26dopt%3DAbstract Authors: Katkov II In the companion paper, we discussed in details proper linearization, calculation of the inactive osmotic volume, and analysis of the results on the Boyle- vant' Hoff plots. In this Letter, we briefly address some common errors and misconceptions in osmotic modeling and propose some approaches, namely: 1) inapplicability of the Kedem-Katchalsky formalism model in regards to the cryobiophysical reality, 2) calculation of the membrane hydraulic conductivity L(p) in the presence of permeable solutes, 3) proper linearization of the Arrhenius plots for the solute membrane permeability, 4) erroneous use of the term "toxicity" for the cryoprotective agents, and 5) advantages of the relativistic permeability approach (RP) developed by us vs. traditional ("classic") 2-paramet... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4578995 Tue, 01 Mar 2011 00:00:00 +0100 4578995 http://www.medworm.com/index.php?rid=4578994&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21376029%26dopt%3DAbstract Authors: Katkov II The Boyle van't Hoff (BVH) law of physics has been widely used in cryobiology for calculation of the key osmotic parameters of cells and optimization of cryo-protocols. The proper use of linearization of the Boyle - vant'Hoff relationship for the osmotically inactive volume (v(b)) has been discussed in a rigorous way in (Katkov, Cryobiology, 2008, 57:142-149). Nevertheless, scientists in the field have been continuing to use inappropriate methods of linearization (and curve fitting) of the BVH data, plotting the BVH line and calculation of v(b). Here, we discuss the sources of incorrect linearization of the BVH relationship using concrete examples of recent publications, analyze the properties of the correct BVH line (which is unique for a given v(b)), provide approp... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4578994 Tue, 01 Mar 2011 00:00:00 +0100 4578994 http://www.medworm.com/index.php?rid=4525420&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21349262%26dopt%3DAbstract This study demonstrates that while the vacuum dried spermatozoa were immotile and had compromised plasma membrane integrity, they were capable of fertilization using ICSI and could support embryo development to the morula stage. PMID: 21349262 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4525420 Mon, 21 Feb 2011 00:00:00 +0100 4525420 http://www.medworm.com/index.php?rid=4525424&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315706%26dopt%3DAbstract Authors: León-Quinto T, Simón MA, Sánchez A, Martín F, Soria B Cryobanking skin samples permit preserving a maximum of genetic representation from the population biodiversity. This is a relevant aspect for threatened species, potentially menaced by an epizooty and from which it is difficult to obtain gametes. As a first step for properly cryobanking skin samples of a given species, the optimal conditions of culture and freezing have to be studied by covering a broad range of possibilities. This paper presents, for the first time, a systematic study of such conditions for the Iberian lynx (Lynx pardinus). To that end, we have analyzed twenty different culture conditions and fifteen different freezing solutions for skin explants, as well as three freezing solutions for isolated cells... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4525424 Sat, 19 Feb 2011 00:00:00 +0100 4525424 http://www.medworm.com/index.php?rid=4525421&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338597%26dopt%3DAbstract Authors: Bellas J, Paredes E Among the most widely used biological techniques in marine pollution assessment, the sea-urchin embryo-larval bioassay is in an advanced developmental stage. Cryopreservation might help to overcome the problem of the spawning seasonality and therefore strengthen the use of those embryo-larval bioassays. This work investigates different factors influencing cryopreservation of sea-urchin embryos, including the cooling rates and holding temperatures, the seeding, or the impact of plunging into liquid nitrogen. The blastula stage yielded better results than the fertilised egg, and the most effective cryoprotectants combination was dimethyl sulfoxide 1.5 M plus trehalose 0.04 M. The optimised protocol developed begins with an initial hold at 4°C for 2 min, foll... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4525421 Fri, 18 Feb 2011 00:00:00 +0100 4525421 http://www.medworm.com/index.php?rid=4525423&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315707%26dopt%3DAbstract Authors: Ferrigno A, Rizzo V, Boncompagni E, Bianchi A, Gringeri E, Neri D, Richelmi P, Freitas I, Cillo U, Vairetti M We previously reported that machine perfusion (MP) performed at 20°C enhanced the preservation of steatotic rat livers. Here, we tested whether rat livers retrieved 30min after cardiac arrest (NHBDs) were better protected by MP at 20°C than with cold storage. We compared the recovery of livers from NHBDs with organs obtained from heart beating donors (HBDs) preserved by cold storage. MP technique: livers were perfused for 6h with UW-G modified at 20°C. Cold storage: livers were perfused in situ and preserved with UW solution at 4°C for 6h. Both MP and cold storage preserved livers were reperfused with Krebs-Heinselet buffer (2h at 37°C). AST and LDH release and mi... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4525423 Wed, 16 Feb 2011 00:00:00 +0100 4525423 http://www.medworm.com/index.php?rid=4525422&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21335000%26dopt%3DAbstract In this study, we assessed the use of disaccharides, antioxidants and caspase inhibitors for cryopreservation of AFSCs in combination with a reduced concentration of Me(2)SO. The thawed cells were tested for viability with MTT assays and a growth curve was created to measure population doubling time. In addition, we performed flow cytometry analysis for cell surface antigens, RT-PCR for mRNA expression of stem cell markers, and assays to determine the myogenic differentiation potential of the cells. A statistically significant (p<0.05) increase in post-thawed cell viability in solutions containing trehalose, catalase and zVAD-fmk with 5% Me(2)SO was observed. The solutions containing trehalose and catalase with 5% or 2.5% (v/v) Me(2)SO produced results similar to those for the control (... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4525422 Tue, 15 Feb 2011 00:00:00 +0100 4525422 http://www.medworm.com/index.php?rid=4470808&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21277294%26dopt%3DAbstract Authors: Elliott JA PMID: 21277294 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4470808 Fri, 28 Jan 2011 00:00:00 +0100 4470808 http://www.medworm.com/index.php?rid=4470809&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276438%26dopt%3DAbstract In conclusion, addition of oligosaccharide can efficiently reduce lesions of freezing on red blood cell membrane in the presence of glucose and dextran. PMID: 21276438 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4470809 Wed, 26 Jan 2011 00:00:00 +0100 4470809 http://www.medworm.com/index.php?rid=4470811&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21272569%26dopt%3DAbstract CONCLUSIONS: Oocyte cryopreservation is associated with molecular injury associated with the decrease of stored mRNA. However the V/W protocol is more conservative than SF/RT resulting in a level of mRNA sufficient to maintain biologic functions in the subsequent fertilized oocyte. PMID: 21272569 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4470811 Tue, 25 Jan 2011 00:00:00 +0100 4470811 http://www.medworm.com/index.php?rid=4470810&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21272570%26dopt%3DAbstract Authors: Elizabeth Tymczyszyn E, Gerbino E, Illanes A, Gómez-Zavaglia A In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-dried samples were stored at 5 and 25°C for different periods of time. After desiccation, freeze-drying or storage, samples were rehydrated and bacterial plate counts were determined. According to the results obtained, all galacto-oligosaccharides assays demonstrated to be highly efficient in the preservation of L. bulgaricus. The higher content of galacto-oligosaccharides in the commercial products w... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4470810 Tue, 25 Jan 2011 00:00:00 +0100 4470810 http://www.medworm.com/index.php?rid=4400858&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21262212%26dopt%3DAbstract In this study, we address these issues by determining the cytotoxicity kinetics for dimethylsulfoxide (Me(2)SO) and 1,2-propanediol (PD) on alginate-encapsulated βTC-tet mouse insulinomas for a range of concentrations and temperatures. Cytotoxicity kinetics were also determined for two cocktails, DPS (3M Me(2)SO + 3M PD + 0.5M sucrose) and PEG400 (1M Me(2)SO + 5M PD + 0.34 M poly(ethylene)glycol with M.W. of 400). PD was found to be more cytotoxic than Me(2)SO at higher concentrations and temperatures. This was reflected in PEG400 being more cytotoxic at room temperature than PEG400 at 4°C or DPS at either temperature. Addition of non-permeating solutes increased the cytotoxicity of cocktails. Furthermore, results indicate that CPA cytotoxicity may not be additive and that combining CPAs... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4400858 Fri, 21 Jan 2011 00:00:00 +0100 4400858 http://www.medworm.com/index.php?rid=4400857&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21262213%26dopt%3DAbstract Authors: Gurina TM, Pakhomov AV, Kyryliuk AL, Bozhok GA A long course of anticancer therapy may lead to testicular steroidogenesis destruction. Cryopreservation of testicular interstitial cells (TIC) would be a strategy to protect hormonal and fertile potential of pre-pubertal boys treated with chemo- or radiotherapy. The aim of this research was to optimize protocols for freezing of TIC. Essential physical processes associated with the presence of dimethyl sulphoxide (Me2SO) in the cryoprotectant solution take place at the temperatures below -60°С. These processes are the eutectic crystallization at the stage of freezing and the recrystallization before the melting of the eutectic mixture at the stage of heating. Both of the processes affect the viability of the cells subjected to c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4400857 Fri, 21 Jan 2011 00:00:00 +0100 4400857 http://www.medworm.com/index.php?rid=4400859&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241687%26dopt%3DAbstract Authors: Johnson LN, Winter KM, Reid S, Hartkopf-Theis T, Marks DC Platelets prepared in plasma can be frozen in 6% dimethyl sulfoxide (Me(2)SO) and stored for extended periods at -80°C. The aim of this study was to reduce the plasma present in the cryopreserved product, by substituting plasma with platelet additive solution (PAS; SSP+), whilst maintaining in vitro platelet quality. Buffy coat-derived pooled leukoreduced platelet concentrates were frozen in a mixture of SSP+, plasma and 6% Me(2)SO. The platelets were concentrated, to avoid post-thaw washing, and frozen at -80°C. The cryopreserved platelet units (n=9) were rapidly thawed at 37°C, reconstituted in 50% SSP+/plasma and stored at 22°C. Platelet recovery and quality were examined 1 and 24h post-thaw and compared to the p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4400859 Sat, 15 Jan 2011 00:00:00 +0100 4400859 http://www.medworm.com/index.php?rid=4400860&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21232534%26dopt%3DAbstract In this study, a novel antifreeze protein gene from the desert beetle Anatolica polita (named as Apafp752) was expressed in a high level in Escherichia coli strain BL21 (DE3). An approximately 30kDa fusion protein thioredoxin (Trx)-ApAFP752 was purified through Ni-NTA affinity chromatography and gel filtration chromatography. The activity of the purified fusion protein Trx-ApAFP752 was analyzed by thermal hysteresis activity (THA) and cryoprotection assay. The results suggested that Trx-ApAFP752 conferred freeze resistance on bacterium in a concentration- and time-dependent manner and the cryoprotective effect increased under alkaline conditions. Circular Dichroism (CD) spectrum analysis showed that the recombinant protein of ApAFP752 possessing β-sheet as the main structure was stable un... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4400860 Tue, 11 Jan 2011 00:00:00 +0100 4400860 http://www.medworm.com/index.php?rid=4337591&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21199643%26dopt%3DAbstract In conclusion, sperm cryopreserved in the extender containing 8% LDL in place of egg yolk exhibited the greatest percentages of post-thaw sperm motility, acrosome integrity and membrane integrity, in comparison with the control, and favored the highest antioxidant activities of CAT, GSH-Px and GSH in comparison with other groups. The replacement of egg yolk by LDL in the composition of extenders was beneficial for bull sperm cryopreservation. PMID: 21199643 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4337591 Fri, 31 Dec 2010 00:00:00 +0100 4337591 http://www.medworm.com/index.php?rid=4337592&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176772%26dopt%3DAbstract This study demonstrates that the protocol established for high-throughput cryopreservation of blue catfish sperm can provide commercially relevant quantities and quality of sperm with stable fertility for hybrid catfish production and provides a model for establishment of commercial-scale approaches for other aquatic species. PMID: 21176772 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4337592 Sun, 19 Dec 2010 00:00:00 +0100 4337592 http://www.medworm.com/index.php?rid=4276449&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168399%26dopt%3DAbstract The objective of this study was to assess the in vitro meiotic maturation of chousingha (four-horned antelope) oocytes following vitrification using open pulled straw (OPS) method. The average number of oocytes recovered per ovary was 65.2. The proportion of oocytes that matured was significantly lower in vitrified oocytes (29.4%) when compared with fresh oocytes (69.3%). The study provides evidence that it is possible to cryopreserve immature oocytes by vitrification collected from the ovaries of chousingha at postmortem and also demonstrates that these cryopreserved oocytes retain their potential to undergo in vitro meiotic maturation. PMID: 21168399 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276449 Thu, 16 Dec 2010 00:00:00 +0100 4276449 http://www.medworm.com/index.php?rid=4276448&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168400%26dopt%3DAbstract In conclusion, we could demonstrate that hypothermia protects cardiomyocytes during oxidative stress by preventing apoptosis via inhibiting mitochondrial dysfunction and DNA damage. PMID: 21168400 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276448 Thu, 16 Dec 2010 00:00:00 +0100 4276448 http://www.medworm.com/index.php?rid=4276447&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168401%26dopt%3DAbstract Authors: Nahiduzzaman M, Hassan MM, Khanam UH, Mamun SN, Hossain MA, Tiersch TR The present study focused on development of a sperm cryopreservation protocol for the critically endangered olive barb Puntius sarana (Hamilton, 1822) collected from two stocks within Bangladesh and reared in the Fisheries Field Laboratory, Bangladesh Agricultural University (BAU). The sperm were collected in Alsever's solution prepared at 296 mOsmol kg(-1). Sperm were activated with distilled water (24 mOsmol kg(-1)) to characterize motility. Maximum motility (90%) was observed within 15 s after activation, and sperm remained motile for 35 s. Sperm activation was evaluated in different osmolalities and motility was completely inhibited when osmolality of the extender was ⩾ 287 mOsmol kg(-1). To evaluate ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276447 Thu, 16 Dec 2010 00:00:00 +0100 4276447 http://www.medworm.com/index.php?rid=4276446&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168402%26dopt%3DAbstract Authors: Slotsbo S, Hansen LM, Holmstrup M The slug Arion lusitanicus Mabille (Gastropoda: Pulmonata: Arionidae) is an invasive species which has spread to most parts of Europe. The area of origin is unknown, but A. lusitanicus seems to cope well with the local conditions in the countries to which it has migrated. It spreads rapidly, occurs often in high densities and has become a serious pest in most European countries. Therefore there is an urgent need for better knowledge of the ecophysiology of Arion lusitanicus, such as the influence of climatic conditions, in order to develop prognostic models and strategies for novel pest management practises. The aim of our study was to investigate the influence of subzero temperatures in relation to winter survival. A. lusitanicus is shown to ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276446 Thu, 16 Dec 2010 00:00:00 +0100 4276446 http://www.medworm.com/index.php?rid=4276450&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21156167%26dopt%3DAbstract In conclusion, the antioxidant defensive capacity of the GSH is severely altered by the freeze-thawing process. The addition of GSH to the freezing and thawing extender could be of partial and limited benefit in improving the function of frozen human spermatozoa. PMID: 21156167 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276450 Sat, 11 Dec 2010 00:00:00 +0100 4276450 http://www.medworm.com/index.php?rid=4276451&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21145884%26dopt%3DAbstract Authors: Gutiérrez-Pérez O, Juárez-Mosqueda ML, Mota D, Trujillo ME The perinuclear theca (PT) is a cytoskeletal structure that surrounds the mammal sperm nucleus which must be disrupted once the sperm has penetrated the oocyte to permit normal chromatin decondensation and formation of male pronucleus. F-actin is a thermo sensitive protein found in the equatorial segment which is involved in the stability of PT. It has been reported that cryopreservation induces alterations in nuclear decondensation of spermatozoa, which have been interpreted as an over condensation. The aims of the present study were identified the presence of changes in sperm sPT integrity of frozen-thawed boar spermatozoa and its effect in sperm nuclei decondensation; and whether changes in the actin cytoskeleton... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4276451 Thu, 09 Dec 2010 00:00:00 +0100 4276451 http://www.medworm.com/index.php?rid=4218132&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21114971%26dopt%3DAbstract Authors: Seki S, Kouya T, Tsuchiya R, Valdez Jr DM, Jin B, Koshimoto C, Kasai M, Edashige K As a step to develop a cryopreservation method for zebrafish oocytes, we investigated the cryobiological properties of immature oocytes at stage III by examining their ability to mature and to develop into hatching embryos after fertilization. When oocytes were chilled at -5 °C for 30 min, the maturation rate decreased, but the rates of fertilization and hatching were not significantly different from those of controls. When oocytes were exposed to hypotonic solutions for 60 min at 25 °C, the rates of maturation, fertilization, and hatching decreased in a solution with 0.16 Osm/kg or below. When oocytes were exposed to hypertonic solutions (containing sucrose) at 25 °C for 30 min, the maturati... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4218132 Fri, 26 Nov 2010 00:00:00 +0100 4218132 http://www.medworm.com/index.php?rid=4218145&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112321%26dopt%3DAbstract Authors: Beirão J, Cabrita E, Pérez-Cerezales S, Martínez-Páramo S, Herráez MP The evaluation of the motility data obtained with a CASA system, applying a Two-Step Cluster analysis, identified in seabream sperm 3 different sperm subpopulations that correlated differently with embryo hatching rates. Hence, we designed an experiment to understand the effect of the application of different cryopreservation protocols in these sperm motility-based subpopulations. We analyzed Sparus aurata frozen/thawed semen motility 15, 30, 45 and 60 seconds after activation, using CASA software. Different protocols were applied for cryopreservation: three different cryoprotectants (Dimethyl Sulfoxide (Me(2)SO), Ethylene Glycol (EG) and Propylene Glycol (PG)) each at two different concentrations and t... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4218145 Thu, 25 Nov 2010 00:00:00 +0100 4218145 http://www.medworm.com/index.php?rid=4218138&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112322%26dopt%3DAbstract Authors: Tollner TL, Dong Q, Vandevoort CA The preservation of the genetic diversity of captive populations of rhesus monkeys is critical to the future of biomedical research. Cryopreservation of rhesus macaque sperm is relatively simple to perform, yields high post-thaw motility, and theoretically, provides via artificial insemination (AI) a way to easily transfer genetics among colonies of animals. In the interest of optimizing semen cryopreservation methods for use with vaginal AI, we evaluated the ability of frozen-thawed rhesus sperm to penetrate periovulatory cervical mucus (CM). Motile sperm concentration of pre-freeze ("fresh") and post-thawed ("thawed") samples from 5 different males were normalized for both computer assisted sperm motion analysis and CM penetration experiment... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4218138 Thu, 25 Nov 2010 00:00:00 +0100 4218138 http://www.medworm.com/index.php?rid=4166187&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21055397%26dopt%3DAbstract Authors: Mazur P, Seki S There is great interest in achieving reproducibly high survivals of mammalian oocytes (especially human) after cryopreservation, but the results to date have not matched the interest. A prime cause of cell death is the formation of more than trace amounts of intracellular ice, and one strategy to avoid it is vitrification. In vitrification procedures, cells are loaded with high concentrations of glass-inducing solutes and cooled to -196°C at rates high enough to presumably induce the glassy state. In the last decade, several devices have been developed to achieve very high cooling rates. Nearly all in the field have assumed that the cooling rate is the critical factor. The purpose of our study was to test that assumption by examining the consequences of coolin... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4166187 Tue, 02 Nov 2010 00:00:00 +0100 4166187 http://www.medworm.com/index.php?rid=4166186&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21055398%26dopt%3DAbstract Authors: Wang X, Xu H A new cell cryopreservation strategy for cell-assembling constructs was proposed. With this strategy, different concentrations of dimethysulfoxide (DMSO) and dextran-40 were directly incorporated into the cell/gelatin/alginate systems, prototyped according to a predesigned structure, cryopreserved at -80°C for 10 days and followed a thawing process at 17°C. The rheological properties, bonding water contents and melting points of the gelatin/alginate hydrogel systems were changed with the addition of different amounts of DMSO. The microscopy analysis, (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide (MTT) and hematoxylin and eosin (HE) staining indicated that the cell numbers were progressively in a selected DMSO concentration range. With DMSO 5% (... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4166186 Tue, 02 Nov 2010 00:00:00 +0100 4166186 http://www.medworm.com/index.php?rid=4166185&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21055399%26dopt%3DAbstract This study shows that both the membrane and cytoskeleton, as a system, are involved in the osmotic and freeze/thaw-induced responses of the mammalian cells. PMID: 21055399 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4166185 Tue, 02 Nov 2010 00:00:00 +0100 4166185 http://www.medworm.com/index.php?rid=4139582&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036162%26dopt%3DAbstract Authors: Nakatsuka S, Yashiro H, Inoue M, Kuribayashi S, Kawamura M, Izumi Y, Tsukada N, Yamauchi Y, Hashimoto K, Iwata K, Nagasawa T, Lin YS Regarding cryoablation for the malignant lung tumors, multiple trials of the freeze-thaw process have been made, and we considered it necessary to view and analyze the freeze-thaw process as a freeze-thaw sequence. We caused the sequence in a porcine lung in vivo by using an acicular, cylindrical stainless-steel probe as the heat source for the freeze-thaw sequence and cooling to -150°C with super high-pressure argon gas by causing the Joule-Thompson effect phenomenon at the tip of the probe. In this experiment, we examined the sequence by measuring the temperature and using the isothermal curve and the freezing function. As a result, it was dem... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4139582 Fri, 29 Oct 2010 00:00:00 +0100 4139582 http://www.medworm.com/index.php?rid=4139583&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21034728%26dopt%3DAbstract Authors: Ohsaka Y, Nishino H Insulin-like growth factor-I and insulin induce the production of phospho-Ser-166 MDM2, a target of Akt, and influence the formation of the MDM2 complex. The glycolipid hormone insulin differentially activates phosphatidylinositol 3-kinase (PI3K)/Akt pathways in 3T3-L1 (L1) adipocytes incubated at 19°C. Responses of L1 adipocytes to different temperature changes and their regulatory mechanisms are poorly understood. We exposed L1 adipocytes to cooling and subsequent rewarming in the presence or absence of wortmannin, a PI3K inhibitor, or mithramycin A, a transcription inhibitor, and examined the induction of phospho-Ser-166 MDM2 and MDM2 and the subcellular formation of the MDM2 complex using western blot analysis. Exposure to 28 and 18°C induced phospho-... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4139583 Tue, 26 Oct 2010 00:00:00 +0100 4139583 http://www.medworm.com/index.php?rid=4123238&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20977900%26dopt%3DAbstract Authors: Yu SO, Brown A, Middleton AJ, Tomczak MM, Walker VK, Davies PL Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an order of magnitude more TH than previously characterized AFPs, we have now determined their activities in ice restructuring inhibition (IrI) assays. IrI activities of three TH-hyperactive AFPs and three less TH-active AFPs varied over an 8-fold range. There was no obvious correlation between high TH activity and high IrI activity. However, the use of mutant AFPs demonstrated that severe disruption of ice-... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4123238 Sat, 23 Oct 2010 00:00:00 +0100 4123238 http://www.medworm.com/index.php?rid=4088296&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20955693%26dopt%3DAbstract Authors: Benson JD, Bagchi A, Han X, Critser JK, Woods EJ Partial phase diagrams are of considerable utility in the development of optimized cryobiological procedures. Recent theoretical predictions of the melting points of ternary solutions of interest to cryobiology have caused us to re-examine measurments that our group made for the ethylene-glycol-sodium chloride-water phase diagram. Here we revisit our previous experiments by measuring melting points at five ethylene-glycol to sodium chloride ratios (R values; R=5, 10, 15, 30, and 45) and five levels of concentration for each ratio. Melting points were averaged from three measurements and plotted as a function of total solute concentration for each R value studied. The new measurements differed from our original experimental value... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4088296 Thu, 14 Oct 2010 23:00:00 +0100 4088296 http://www.medworm.com/index.php?rid=4088295&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20959171%26dopt%3DAbstract Authors: Sisunandar , Sopade PA, Samosir YM, Rival A, Adkins SW Cryopreservation of coconut can be used as a strategy to back up the establishment of living collections which are expensive to maintain and are under constant threat from biotic and abiotic factors. Unfortunately, cryopreservation protocols still need to be developed that are capable of producing a sizeable number of field-grown plants. Therefore, we report on the development of an improved cryopreservation protocol which can be used on a wide range of coconut cultivars. The cryopreservation of zygotic embryos and their recovery to soil-growing plants was achieved through the application of four optimised steps viz.: (i) rapid dehydration; (ii) rapid cooling; (iii) rapid warming and recovery in vitro and (iv) acclimatisat... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4088295 Thu, 14 Oct 2010 23:00:00 +0100 4088295 http://www.medworm.com/index.php?rid=4088297&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20951122%26dopt%3DAbstract Authors: Tuncer PB, Bucak MN, Büyükleblebici S, Sarıözkan S, Yeni D, Eken A, Akalın PP, Kinet H, Avdatek F, Fidan AF, Gündoğan M The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose(®) extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose(®). Insemination doses were processed that each 0.25-ml straw contained 15 x 10(6) sperm. The addition of antioxidants did not present any significant effect on the percentages o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4088297 Tue, 12 Oct 2010 23:00:00 +0100 4088297 http://www.medworm.com/index.php?rid=4077279&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20940007%26dopt%3DAbstract Authors: Jiang J, Goel R, Schmechel S, Vercellotti G, Forster C, Bischof J Cryosurgery is increasingly being used to treat prostate cancer; however, a major limitation is local recurrence of disease within the previously frozen tissue. We have recently demonstrated that tumor necrosis factor alpha (TNF-α), given 4 hours prior to cryosurgery can yield complete destruction of prostate cancer within a cryosurgical iceball. The present work continues the investigation of the cellular and molecular mechanisms and dynamics of TNF-α enhancement on cryosurgery. In vivo prostate tumor (LNCaP Pro 5) was grown in a dorsal skin fold chamber (DSFC) on a male nude mouse. Intravital imaging, thermography, and post-sacrifice histology and immunohistochemistry were used to assess iceball location and... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4077279 Fri, 08 Oct 2010 23:00:00 +0100 4077279 http://www.medworm.com/index.php?rid=4077278&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20940008%26dopt%3DAbstract This study was designed to determine the order of toxicity of five commonly used cryoprotectant agents as well as interactions that occur between them. Isolated porcine articular chondrocytes were exposed to individual cryoprotectant agents and combinations of these agents at 1M and 3M concentrations for 5 minutes and 120 minutes. Cell viability was determined using membrane integrity dyes and a metabolic activity assay. Subsequently, a regression analysis based study was undertaken to extract the maximum amount of information from this data. Results of this study demonstrated that all 1M solutions were minimally toxic. The 3M solutions demonstrated varying toxicity after 120 minutes. Ethylene glycol and glycerol were less toxic than propylene glycol, dimethyl sulfoxide, and formamide. Com... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4077278 Fri, 08 Oct 2010 23:00:00 +0100 4077278 http://www.medworm.com/index.php?rid=4062183&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20937267%26dopt%3DAbstract Authors: Tian P, Legge M Previous immunolocalisation studies using intact cells have identified modification of the cytoskeleton by cryoprotectants. In the present study we have used a proteomics approach to directly resolve the interactive effects of 3T3-LI cells exposed to two cryoprotectants, dimethyl sulphoxide (Me(2)SO) and 1,2-propanediol (PROH) in 5,10, 20 and 50 (v/v) per cent solutions respectively. Two-dimensional protein electrophoresis and western blot analysis of the cell extracts identified a range of immunoreactive actin fragments with varying molecular weights and isoelectric points at all cryoprotectant concentrations. The addition of either 10mM L-cysteine or reduced glutathione to the cells prior to cryprotectant exposure modified the actin fragmentation. In this pre... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4062183 Thu, 07 Oct 2010 23:00:00 +0100 4062183 http://www.medworm.com/index.php?rid=4003515&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20869362%26dopt%3DAbstract Authors: Koh IC, Yokoi KI, Tsuji M, Tsuchihashi Y, Ohta H In the present study, we examined methods for the cryopreservation of Epinephelus septemfasciatus spermatozoa. The percent motility, average path velocity, and linearity of movement (LIN) of fresh and corresponding post-thaw sperm were evaluated. Sperm motility was investigated using computer-assisted sperm analysis. Five percent dimethyl sulphoxide (Me(2)SO) with 95% fetal bovine serum (FBS) was the most successful cryoprotectant diluent with a comparative post-thaw motility of 77.6 ± 8.5%; 5% dimethyl formamide was also effective. Fetal bovine serum was significantly better as an extender when compared with artificial seminal plasma, glucose, and trehalose solution. Sperm tolerated a wide range of cooling rates (from 27.1 t... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4003515 Tue, 21 Sep 2010 23:00:00 +0100 4003515 http://www.medworm.com/index.php?rid=4003793&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20863822%26dopt%3DAbstract The objective of this study is to assess the immunological effects of cryotreated whole tumour cells on dendritic cells (DC) maturation and function using an in vitro model.Prostate cancer cells were cooled using Endocare cryo-system to mimic temperatures achieved during clinical cryotherapy. Human DC were prepared from cluster of differentiation (CD) 14 monocytes and matured with lipopolysaccharide (LPS). Cryotreated cancer cells were added to DC on day 3. On day 7, DC were harvested and phenotyped. Cytokine gene expression was assessed using real time quantitative polymerase chain reaction (RT-PCR). Functional activity of DC was assessed in allogenic mixed lymphocyte reaction (MLR) and the molecular changes using gene microarray technology.There was statistically significant upregulation... Cryobiology journals http://www.medworm.com/rss/comments.php?id=4003793 Sun, 19 Sep 2010 23:00:00 +0100 4003793 http://www.medworm.com/index.php?rid=4003586&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20863823%26dopt%3DAbstract CONCLUSION: The results of this study challenge the idea that oncotic support is not a fundamental requirement of static organ storage. Furthermore, our data suggests that HES is an effective oncotic agent for use in our intraluminal nutrient-rich preservation solution, while Dextran 70 is not. PMID: 20863823 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4003586 Sun, 19 Sep 2010 23:00:00 +0100 4003586 http://www.medworm.com/index.php?rid=4003876&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20858475%26dopt%3DAbstract In conclusion, it is not recommended to centrifuge the ejaculates from collared peccaries prior to conducting the cryopreservative procedures using a Tris-based extender supplemented with fructose or glucose. PMID: 20858475 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=4003876 Fri, 17 Sep 2010 23:00:00 +0100 4003876 http://www.medworm.com/index.php?rid=3981417&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20833164%26dopt%3DAbstract This study was conducted to determine the effects of methionine, inositol and carnitine on sperm (sperm motility, abnormality, DNA integrity and in vivo fertility) and oxidative stres parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze-thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5°C and then frozen in 0.25 ml French straws. Frozen straws were then thawed individually at 37°C for 20 sec in a water bath... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3981417 Wed, 08 Sep 2010 23:00:00 +0100 3981417 http://www.medworm.com/index.php?rid=3954715&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20826129%26dopt%3DAbstract Authors: Olmo A, Risco R PMID: 20826129 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3954715 Thu, 02 Sep 2010 23:00:00 +0100 3954715 http://www.medworm.com/index.php?rid=3899865&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20727872%26dopt%3DAbstract Conclusion Our data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal. PMID: 20727872 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3899865 Tue, 17 Aug 2010 23:00:00 +0100 3899865 http://www.medworm.com/index.php?rid=3899864&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20727873%26dopt%3DAbstract In conclusion, for oocytes that had failed to mature, vitrification gave better survival, maturation, fertilization and also cleavage rates than the slow cooling protocols. Four cells embryos were obtained only from vitrified in vitro matured MI oocytes. PMID: 20727873 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3899864 Tue, 17 Aug 2010 23:00:00 +0100 3899864 http://www.medworm.com/index.php?rid=3853924&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20691677%26dopt%3DAbstract In this study, the power to be dissipated is calculated individualizing the trend of temperature on the Pulse Tube cold head and inside the cell test tubes. The control system is not based on an historical series of data so it can be used in different operative conditions. The obtained temperature curve is in good agreement with the theoretical values, with errors within those accepted by commercial systems. The Pulse Tube cryorefrigerator may represent a valid alternative solution to programmable liquid nitrogen freezer, especially where nitrogen's supply is difficult or extremely expensive. PMID: 20691677 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3853924 Tue, 03 Aug 2010 23:00:00 +0100 3853924 http://www.medworm.com/index.php?rid=3853932&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20691649%26dopt%3DAbstract In conclusion, our results suggest that lyophilized somatic cells can direct embryonic development up to blastocyst stage after WCICI into pig oocytes. Treatment of embryos, produced from lyophilized somatic cells, with TSA can further increase their in vitro developmental potential. PMID: 20691649 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3853932 Wed, 28 Jul 2010 23:00:00 +0100 3853932 http://www.medworm.com/index.php?rid=3791606&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20654607%26dopt%3DAbstract Authors: Chen YK, Liu QH, Li J, Xiao ZZ, Xu SH, Shi XH, Ma DY The aim of this study was to determine the effect of long-term cryopreservation on physiological characteristics, the antioxidant activities and lipid peroxidation of red seabream sperm which were respectively cryopreserved with 15% dimethylsulfoxide (Me(2)SO) for 1 month, 13 months, 26 months, 48 months and 73 months. The motility and fertility of post-thaw sperm decreased with the storage time going on. The highest motility (87.67+/-2.52%) was obtained in sperm cryopreserved for 1 month and the lowest (50.67+/-5.31%) was in sperm for 73 months. There were no significant differences (P < 0.05) in fertilization rates of sperm cryopreserved for 1 month (71.33+/-8.84%), 13 months (69.22+/-1.02%) and 26 months (60.33+/-2.33%... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3791606 Mon, 19 Jul 2010 23:00:00 +0100 3791606 http://www.medworm.com/index.php?rid=3791605&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20654608%26dopt%3DAbstract The objectives were to: 1) evaluate the acute toxicity of methanol, 2-methoxyethanol (ME), dimethyl sulfoxide (Me(2)SO), N, N- dimethylacetamide (DMA), N, N,-dimethyl formamide (DMF), and glycerol with concentrations of 5, 10, and 15% for 60 min of incubation at 4 degrees C; 2) evaluate cooling rates from 5 to 25 degrees C/min for freezing and their interaction with cryoprotectants, and 3) test fertility of thawed sperm cryopreserved with selected cryoprotectants and associated cooling rates. Evaluation of cryoprotectant toxicity showed that methanol and ME (5 and 10%) did not change the sperm motility after 30 min; Me(2)SO, DMA, and DMF (10 and 15%) and glycerol (5, 10 and 15%) significantly decreased the motility of sperm within 1 min after mixing. Based on these results, methanol and ME... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3791605 Mon, 19 Jul 2010 23:00:00 +0100 3791605 http://www.medworm.com/index.php?rid=3791604&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20654609%26dopt%3DAbstract This study demonstrates that our experimental data-based method is a valid one with clear physical interpretations, convenient expressions and a more extensive application room than traditional ones. PMID: 20654609 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3791604 Mon, 19 Jul 2010 23:00:00 +0100 3791604 http://www.medworm.com/index.php?rid=3791603&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20654610%26dopt%3DAbstract Authors: Coloma MA, Gómez-Brunet A, Velázquez R, Toledano-Díaz A, López-Sebastián A, Santiago-Moreno J Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex spermatozoa collected by electroejaculation. The effect of the interaction glycerolization temperature x plasma testosterone concentration was also evaluated. The spermatozoa used in this work came from six adult ibexes maintained in captivity. Each ejaculate was divided into two aliquots in a Tris-egg yolk-based medium. One fraction was subjected to single ste... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3791603 Mon, 19 Jul 2010 23:00:00 +0100 3791603 http://www.medworm.com/index.php?rid=3776929&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20638376%26dopt%3DAbstract Authors: Butts IA, Litvak MK, Kaspar V, Trippel EA Broodstock selection programs are currently underway for Atlantic cod (Gadus morhua). To complement and further these selection programs we need to develop sperm cryopreservation procedures. This will allow genomic DNA from males from selected individuals or stocks to be frozen and conserved in perpetuity. In our study we used a full factorial ANOVA design to examine the effects of diluent (Mounib's sucrose-based diluent, Hanks' Balanced Salt Solution, Mounib's sucrose-based diluent + hen's egg yolk, and Hanks' Balanced Salt Solution + hen's egg yolk), cryoprotectant (propylene glycol, dimethyl sulphoxide, and glycerol), and freezing rate (-2.5, -5.0, -7.5, and -10.0 degrees C/min) on motility of cod frozen-thawed sperm. Sperm velocity... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3776929 Wed, 14 Jul 2010 23:00:00 +0100 3776929 http://www.medworm.com/index.php?rid=3776928&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20638377%26dopt%3DAbstract Authors: Wang X, Wang F, Wu X, Zhao X, Liu J, Huang C, Dong Q The present study employed cryomicroscopy to derive an optimal sperm freezing protocol for guppy (Poecilia reticulata) sperm. Evaluation criteria during the freezing-thawing process were assessed for nucleation temperature (Tn), temperature when more than 50% of sperm display bending midpiece (Tb), temperature when more than 80% of sperm stop moving (Tm), thawing temperature (Tt), and post-thaw motility. We compared four different cryoprotectants: 5% N-dimethyl formamide (DMF), 6% methanol (MEOH), 10% dimethyl sulfoxide (DMSO), and 14% glycerol, as well as glycerol at different concentrations of 7-50%; cooling and rewarming rates ranged from 5 to 100 degrees C/min. The protocol that yielded the highest post-thaw motility was... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3776928 Wed, 14 Jul 2010 23:00:00 +0100 3776928 http://www.medworm.com/index.php?rid=3733564&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599881%26dopt%3DAbstract Authors: Kleinhans FW, Seki S, Mazur P We have developed a simple, inexpensive system (< $300 US) for measuring cooling and warming rates of small ( approximately 0.1 mul) aqueous samples at rates as high as 10(5) degrees C/min. The measurement system itself, can track rates approaching one million degrees C/min. For temperature sensing, a Type T thermocouple with 50 mum wire was used. The thermocouple output voltage was read with an inexpensive USB based digital oscilloscope interfaced to a laptop computer, and the raw data were processed with MS Excel. PMID: 20599881 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733564 Thu, 01 Jul 2010 23:00:00 +0100 3733564 http://www.medworm.com/index.php?rid=3733563&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599882%26dopt%3DAbstract Authors: Kimura M, Rabbani Z, Mouraviev V, Tsivian M, Vujaskovic Z, Satoh T, Baba S, Baust JM, Baust JG, Polascik TJ The aim of this study is to investigate the tumor tissue changes in terms of hypoxia and demonstrate its relationship to vascularity and apoptosis following therapeutic cryoablation in a prostate tumor murine model. Total 67 male C57BL/J6 mice were assigned into sham-operation group and cryoablation group. Murine prostate tumors (RM-9) were inoculated subcutaneously in a right hind leg and treated with cryotherapy. Of 30 mice, tumor volumes were measured for 12 days following operation. Of 37 mice, tumor tissues were harvested in 24 hours following operation, and histological/molecular changes were analyzed. Hematoxylin and eosin or immunohistochemical staining were util... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733563 Thu, 01 Jul 2010 23:00:00 +0100 3733563 http://www.medworm.com/index.php?rid=3733562&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599883%26dopt%3DAbstract The objective of the study was to determine the effect of antioxidant addition to the freezing extender on boar semen qualities and fertility capacity. Ejaculates were collected from a previously selected boar and semen samples were processed using the straw freezing procedure. In experiment 1, semen samples were cryopreserved in lactose-egg yolk solution supplemented with various concentrations of cysteine (0,5and10mM) to determinate a cysteine concentration capable of producing a protective effect during cryopreservation. Semen quality (total motility, progressive motility, viability, acrosome integrity and hypoosmotic swelling test) was evaluated after freezing and thawing and then every hour for 3 h. In experiment 2, ejaculates were cryopreserved with lactose-egg yolk extender with or ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733562 Thu, 01 Jul 2010 23:00:00 +0100 3733562 http://www.medworm.com/index.php?rid=3733561&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599884%26dopt%3DAbstract The objective was to examine the effects of IIF on post-thaw viability of both confluent monolayers and suspensions of DPSCs. Confluent DPSC monolayers were assessed for the expression of gap junction protein Connexin-43. IIF was induced on the cryostage and in the methanol bath at different subzero temperatures. Membrane integrity and colony forming ability were assessed post-thaw. Confluent DPSC monolayers expressed Connexin-43. In cell suspensions, 85.9+/-1.7% of cells were damaged after 100% IIF. In cell monolayers, after 100% IIF, only 25.5+/-5.5% and 14.8+/-3.3% of cells were damaged on the cryostage and in the methanol bath respectively. However, DPSC monolayers exposed to 100% IIF showed no colony forming ability. We conclude that confluent monolayers of DPSCs express the gap junct... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733561 Mon, 21 Jun 2010 23:00:00 +0100 3733561 http://www.medworm.com/index.php?rid=3733560&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599885%26dopt%3DAbstract Authors: Hawes TC, Worland MR, Bale JS The process of organismal freezing in the Antarctic limpet, Nacella concinna, is complicated by molluscan biology. Internal ice formation is, in particular, mediated by two factors: (a) the provision of an inoculative target for ice formation in the exposed mucus-secreting foot; and (b) osmoconformity to the marine environment. With regard to the first, direct observations of the independent freezing of pedal mucus support the hypothesis that internal ice formation is delayed by the mucal film. As to the second, ice nucleation parametrics of organismal tissue (head, midgut, gonad, foot) and mucus in both inter- and subtidal populations were characterized by high melting points (range = -4.61 to -6.29 degrees C), with only c.50% of a given sample o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733560 Fri, 18 Jun 2010 23:00:00 +0100 3733560 http://www.medworm.com/index.php?rid=3733559&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599886%26dopt%3DAbstract This report is expected to evoke interests in applying thermodynamics to the investigation of the intracellular ice formation. PMID: 20599886 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733559 Wed, 16 Jun 2010 23:00:00 +0100 3733559 http://www.medworm.com/index.php?rid=3733558&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599887%26dopt%3DAbstract Authors: Chen HH, Clarke DM Phase diagrams of solutions consisting of cryoprotective agents (CPA) are very useful in cryobiology research. Those diagrams depict the points of solution concentrations at corresponding temperatures: one of essential inputs that can be utilized to compute the volume response of cell under freezing process. However, generating such plots is costly and time-consuming. A direct method is proposed in this study to determine the solution concentration of unfrozen parts at multiple subzero temperatures. Measurements of binary solutions, composed of water and sodium chloride, were performed and compared with published data. Ternary solutions, consisting of water, sodium chloride and dimethyl sulfoxide, were also measured. The uniqueness and advantage achieved thr... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733558 Wed, 16 Jun 2010 23:00:00 +0100 3733558 http://www.medworm.com/index.php?rid=3733557&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599888%26dopt%3DAbstract CONCLUSIONS: : Lesions obtained after cryotherapy seem similar and behave as those obtained after other techniques of liver ablation. However, controversial areas still exist and include the optimum number of freeze thaw cycles, the place of inflow occlusion, the potential corrupting effects of intra-lesional or proximal blood vessels on ablation morphology. The influence of these factors on the local recurrences are still not fully understood. PMID: 20599888 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3733557 Wed, 16 Jun 2010 23:00:00 +0100 3733557 http://www.medworm.com/index.php?rid=3691278&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20561514%26dopt%3DAbstract Authors: Amidi F, Farshad A, Khor AK The susceptibility of mammalian spermatozoa to cold shock and freezing damage is due to changes in membrane lipid composition, particularly cholesterol depletion in plasma membrane during cryopreservation. The aim of this study was to investigate the effects of different concentrations of cholesterol-loaded cyclodextrin (CLC) and bovine serum albumin (BSA) on the cryopreservation of goat spermatozoa in Tris-citrate egg yolk extender. Semen was collected from four mature goats and divided into seven aliquots prior to cryopreservation .The first aliquot remained untreated and was mixed with TCG, the second aliquot was mixed with TCG and egg yolk (TCGY), third aliquot was mixed with TCGY and 2.5% BSA (TCGYB) and other aliquots were mixed with TCGYB con... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3691278 Mon, 14 Jun 2010 23:00:00 +0100 3691278 http://www.medworm.com/index.php?rid=3691279&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20558152%26dopt%3DAbstract This study was designed to determine the vitrifyability and glass stability of solutions containing combinations of commonly used CPAs and to document CPA interactions that occur. One hundred and sixty-four multi-CPA combination solutions of 6-9M were evaluated for vitrifyability and glass stability using direct visualization after immersion in liquid nitrogen for 30 minutes and upon warming. Binary and ordinal logistic regression analysis was used to statistically analyze each CPA for its ability to vitrify and its effect on glass stability in multi-component CPA solutions. Propylene glycol has the greatest incremental contribution to vitrification while formamide had the least contribution. A threshold was established whereby the ability of a solution to vitrify could be determined by ca... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3691279 Sun, 13 Jun 2010 23:00:00 +0100 3691279 http://www.medworm.com/index.php?rid=3691280&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20553897%26dopt%3DAbstract We describe a method to determine membrane hydraulic permeability parameters (E(Lp), Lpg) at subzero temperatures from membrane phase behavior data. In order to do this, it was assumed that the measured freezing-induced shift in wavenumber position of the symmetric CH(2) stretching band arising from the lipid acyl chains is proportional to cellular dehydration. Membrane permeability parameters were also determined by analyzing the H(2)O-bending and -libration combination band, which yielded higher values for both E(Lp) and Lpg as compared to lipid band analysis. These differences likely reflect differences between transport of free and membrane-bound water. FTIR allows for direct assessment of membrane properties at subzero temperatures in intact cells. The derived biophysical membrane par... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3691280 Mon, 07 Jun 2010 23:00:00 +0100 3691280 http://www.medworm.com/index.php?rid=3691277&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20566369%26dopt%3DAbstract In this study, Fourier transform infrared spectroscopy with an attenuated total reflection accessory was used to investigate the influence of trehalose on the dehydration kinetics and residual water content of egg phosphatidylcholine liposomes in real time under controlled relative humidity conditions. In the absence of trehalose, the lipids displayed a transition to a more ordered gel phase upon drying. The membrane conformational disorder in the dried state was found to decrease with decreasing relative humidity. Even at a relative humidity as high as 94% the conformational disorder of the lipid acyl chains decreased after evaporation of the bulk water. The presence of trehalose affects the rate of water removal from the system and the lipid phase behavior. The rate of water removal is d... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3691277 Mon, 07 Jun 2010 23:00:00 +0100 3691277 http://www.medworm.com/index.php?rid=3691276&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20570671%26dopt%3DAbstract Authors: Tchir J, Acker JP The maintenance of cell membrane integrity is an absolute minimum criterion for the selection of a successful cryopreservation process; however, it is often used as the sole determinant of cell "viability". Membrane stresses and strains that develop with cell volume fluctuations are only one component of the overall cellular response to freezing. Damage to organelles resulting from excessive concentration of intracellular solutes and/or the alternation of molecular signalling events may affect post-thaw outcomes. As the low temperature response of cells is affected by the presence of cell-cell interactions, the cryopreservation of tissues and tissue model systems would benefit from a more detailed understanding of the sites and mechanisms of cryoinjury. The p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3691276 Wed, 02 Jun 2010 23:00:00 +0100 3691276 http://www.medworm.com/index.php?rid=3626121&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20515679%26dopt%3DAbstract In conclusion, methionine and raffinose play a cryoprotective role against sperm CASA motility, acrosome abnormality and DNA damage. Raffinose 5 mM exhibited antioxidative properties, decreasing MDA levels. Further studies are required to obtain more concrete results on the characterization of microscopic parameters and antioxidant activities in cryopreserved goat sperm with different additives. PMID: 20515679 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3626121 Fri, 28 May 2010 23:00:00 +0100 3626121 http://www.medworm.com/index.php?rid=3626123&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20510224%26dopt%3DAbstract Authors: Menze MA, Chakraborty N, Clavenna M, Banerjee M, Liu XH, Toner M, Hand SC In species whose evolutionary history has provided natural tolerance to dehydration and freezing, metabolic depression is often a pre-requisite for survival. We tested the hypothesis that preconditioning of mammalian cells with 5-aminoimidazole-4-carboxamide-1-b-D-ribofuranoside (AICAR) to achieve metabolic depression will promote greater survivorship during cryopreservation. AICAR is used extensively to stimulate AMP-activated protein kinase (AMPK), which can result in downregulation of biosynthetic processes. We showed that the metabolic interconversion of AICAR was cell-type dependent. Accumulation of 5-aminoimidazole-4-carboxamide-1b-D-ribofuranosyl-5'-monophosphate (ZMP), as well as other metabolite... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3626123 Mon, 24 May 2010 23:00:00 +0100 3626123 http://www.medworm.com/index.php?rid=3626122&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20510225%26dopt%3DAbstract In conclusion, cumulus-enclosed GV bovine oocytes survived vitrification and subsequently developed at higher rates than MII oocytes using Cryotop method and conventional IVF procedure. Theoretical analysis of permeability characteristics and tolerance limits could not explain the low developmental competence of vitrified oocytes. PMID: 20510225 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3626122 Sun, 23 May 2010 23:00:00 +0100 3626122 http://www.medworm.com/index.php?rid=3578540&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20478291%26dopt%3DAbstract Authors: Kaku M, Kamada H, Kawata T, Koseki H, Abedini S, Kojima S, Motokawa M, Fujita T, Ohtani J, Tsuka N, Matsuda Y, Sunagawa H, Hernandes RA, Ohwada N, Tanne K The purpose of this study was to establish a long-term tooth cryopreservation method that can be used for tooth autotransplantation. Human periodontal ligament (PDL) cells were frozen in 10% dimethyl sulfoxide (Me2SO) using a programmed freezer with a magnetic field. Cells were cryopreserved for 7 days at -150 degrees C. Immediately after thawing, the number of surviving cells was counted and the cells were cultured; cultured cells were examined after 48 h. Results indicated that a 0.01mT of a magnetic field, a 15-min hold-time, and a plunging temperature of -30 degrees C led to the greatest survival rate of PDL cells. Based... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3578540 Thu, 13 May 2010 23:00:00 +0100 3578540 http://www.medworm.com/index.php?rid=3578541&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20471968%26dopt%3DAbstract Authors: Ross-Rodriguez LU, Elliott JA, McGann LE Cryopreservation plays a key role in the long-term storage of native and engineered cells and tissues for research and clinical applications. The survival of cells and tissues after freezing and thawing depends on the ability of the cells to withstand a variety of stresses imposed by the cryopreservation protocol. A better understanding of the nature and kinetics of cellular responses to temperature-induced conditions is required to minimize cryoinjury. An interrupted freezing procedure that allows dissection of cryoinjury was used to investigate the progressive damage that occurs to cells during cryopreservation using slow cooling. Simulations of cellular osmotic responses were used to provide interpretation linking states of the cell ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3578541 Wed, 12 May 2010 23:00:00 +0100 3578541 http://www.medworm.com/index.php?rid=3578542&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20471379%26dopt%3DAbstract This study also demonstrates why high cell recovery after cryopreservation could be achieved in the absence of traditional cryoprotectants. PMID: 20471379 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3578542 Tue, 11 May 2010 23:00:00 +0100 3578542 http://www.medworm.com/index.php?rid=3578543&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20470768%26dopt%3DAbstract In this study, experiments were performed to determine these characteristics for immature (germinal vesicle, GV) and in vitro matured (metaphase II, MII) bovine oocytes within 4 degrees C to 37 degrees C, and a new step-wise adding and diluting protocol for ethylene glycol (EG) was developed and verified. Osmotically inactive volumes (Vb) of GV and MII oocytes were calculated to be 16.1% and 26.1%. The membrane permeability of the oocytes to water (Lp) in the presence of EG were between 0.08-0.18 and 0.14-0.28mum/min/atm, and the membrane permeability of the oocytes to solutes (Ps) were between 0.0011-0.0038 and 0.0029-0.0061cm/min for GV and MII oocytes, respectively. The activation energies (Ea) for Lp and Ps in the presence of EG were 3.68 and 6.84kcal/mole for GV oocyte, while 3.62 and... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3578543 Mon, 10 May 2010 23:00:00 +0100 3578543 http://www.medworm.com/index.php?rid=3545806&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20447385%26dopt%3DAbstract In this study, an auto-sampler differential scanning calorimeter (DSC) was used to determine the quaternary phase diagram of the water-ethylene glycol-sucrose-NaCl system. To improve the accuracy of melting point measurement, a "mass redemption" method was also applied for the DSC technique. Base on the analyses of these experimental data, a comparison was made between the two practical approaches to generate phase diagrams of multi-solute solutions from those of single-solute solutions: the summation of cubic polynomial melting point equations versus the use of osmotic virial equations with cross coefficients. The calculated values of the model standard deviations suggested that both methods are satisfactory for characterizing this quaternary system. PMID: 20447385 [PubMed - as suppli... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3545806 Sun, 02 May 2010 23:00:00 +0100 3545806 http://www.medworm.com/index.php?rid=3530618&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20433824%26dopt%3DAbstract Authors: Hegler F, Kappler A Preservation and storage of microbial stock cultures is desirable since the risk of contamination or loss of living cultures is immanent while over long periods mutations accumulate. Generally, it is rather difficult to preserve photosynthetic bacteria due to their sensitive photosynthetic membranes [1]. Phototrophic Fe(II)-oxidizing bacteria face an additional challenge; since they are exposed to light and Fe(II) during growth, they have to cope with radicals from Fenton-reactions of Fe(II)-species, light and water. Therefore, phototrophic Fe(II)-oxidizing strains are thought to be especially susceptible to genetic modifications. Here we provide a simple and fast protocol using glycerol as cryo-protectant to cryopreserve three strains of anoxygenic phototr... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3530618 Mon, 26 Apr 2010 23:00:00 +0100 3530618 http://www.medworm.com/index.php?rid=3524740&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20430019%26dopt%3DAbstract CONCLUSION: : Viability of cold stored liver grafts can be notably augmented by gaseous ex vivo application of low dose CO to the isolated organ. PMID: 20430019 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3524740 Sun, 25 Apr 2010 23:00:00 +0100 3524740 http://www.medworm.com/index.php?rid=3524739&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20430020%26dopt%3DAbstract Authors: Gearing J, Malik KP, Matejtschuk P Dynamic Mechanical Analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analysed and glass transition events demonstrated. In addition, it allows weak glass transitions to be detected clearly in some complex formulations where they can be obscured by eutectic and other strong thermal events when other methods such as DSC or DTA are used. Classical excipients (trehalose, lactose, dextran) were analysed and shown to give reproducible Tg' values, though with values slightly higher than those obtained by DSC.... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3524739 Sun, 25 Apr 2010 23:00:00 +0100 3524739