MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cryobiology' source. Sun, 21 Mar 2010 17:00:52 +0100 http://www.medworm.com/index.php?rid=3385979&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20298686%26dopt%3DAbstract Authors: Fahy GM, Pegg DE PMID: 20298686 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3385979 Mon, 15 Mar 2010 00:00:00 +0100 3385979 http://www.medworm.com/index.php?rid=3379694&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20233586%26dopt%3DAbstract Authors: Dollo VH, Yi SX, Lee Jr RE Indirect chilling injury commonly occurs during long-term exposure to low temperature in many organisms including insects. A previous study revealed increased rates of survival and reduced cold injury in flesh flies, Sarcophaga crassipalpis, that experienced an intermittent pulse of high temperature during a low-temperature regiment. We extended these studies by determining survival rates and ATP levels for flies that had undergone continuous long-term exposure at 0 degrees C versus those experiencing a 24-h warming pulse of either 15 or 20 degrees C. Survival among flies that had undergone a warming pulse was significantly greater than for flies that were maintained continuously at 0 degrees C. Furthermore, ATP levels of flies that had experienced a... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3379694 Sat, 13 Mar 2010 00:00:00 +0100 3379694 http://www.medworm.com/index.php?rid=3379693&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20233587%26dopt%3DAbstract CONCLUSIONS: Oxygenated machine perfusion improves viability of livers after prolonged warm ischemic damage. Elevated perfusion temperature of 21 degrees C reconstitutes the hepatic functional capacity better than perfusion at 4 degrees C or 12 degrees C. PMID: 20233587 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3379693 Sat, 13 Mar 2010 00:00:00 +0100 3379693 http://www.medworm.com/index.php?rid=3379692&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20233588%26dopt%3DAbstract Authors: Sitaula R, Fowler A, Toner M, Bhowmick S The goal of the study was to improve the partial desiccation survival of bovine sperm by decreasing the dehydration induced osmotic injury. The protective role of sorbitol, a polyol, was investigated by (i) studying the osmotic behavior of sperm in hypertonic Tyrode's buffer in the presence of sorbitol and trehalose, (ii) studying the effect of sorbitol and trehalose on sperm motility following partial dehydration. The osmotic behavior studies included the assessment of motility and volumetric responses in the presence of the additives. For the drying experiments, motility was assayed after drying the samples to different end water content followed by immediate rehydration. Compared to the effect of "intracellular + extracellular" treha... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3379692 Sat, 13 Mar 2010 00:00:00 +0100 3379692 http://www.medworm.com/index.php?rid=3379691&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20233589%26dopt%3DAbstract Authors: Graziano G It is pointed out that the pictorial iceberg model, proposed by Frank and Evans in 1945 to explain the origin of the hydrophobic effect and considered to be right by Dias and colleagues, is questionable because the insertion of a nonpolar solute molecule in water does not seem to cause an enhancement of water structure. The solvent-excluded volume effect associated with solute insertion into water is the physical origin of the hydrophobic effect and of its role in cold denaturation of globular proteins. PMID: 20233589 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3379691 Sat, 13 Mar 2010 00:00:00 +0100 3379691 http://www.medworm.com/index.php?rid=3354660&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20214895%26dopt%3DAbstract This study shows that cryopreservation of teeth affects the expression of the FGFR2 gene in cultured PDL cells, which is related to cell growth, cell development, and cell-cell signaling. PMID: 20214895 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3354660 Sat, 06 Mar 2010 00:00:00 +0100 3354660 http://www.medworm.com/index.php?rid=3311694&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20178778%26dopt%3DAbstract In conclusion, combination of EG with OPS gives the best result in terms of better recovery and survival rate, least morphological damages with good developmental competence of vitrified matured buffalo oocytes post thawing. PMID: 20178778 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3311694 Sat, 20 Feb 2010 00:00:00 +0100 3311694 http://www.medworm.com/index.php?rid=3311692&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20178779%26dopt%3DAbstract In this study, we looked at the effect of cryopreservation on breast cancer progenitor cells known as mammospheres, which are derived from the MCF7 breast carcinoma cell line. We focused on the effect of cryopreservation on the cell biology and function of tumor-initiating cells using a standard method of cryopreservation with 15% dimethyl sulfoxide (Me2SO). Cell proliferation and survival was analyzed by alamarBlue solution on cryopreserved cells stored for 1 to 12 weeks and also by the expression of Ki-67. To assess self-renewal, single cells were harvested by limiting dilution procedure and wells were scored once a week. In order to investigate senescence, the activity of beta-galactosidase was detected by histochemical staining. Our results indicate that cryopreservation of breast canc... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3311692 Sat, 20 Feb 2010 00:00:00 +0100 3311692 http://www.medworm.com/index.php?rid=3302910&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20176010%26dopt%3DAbstract In conclusion, incubation with monosaccharide could significantly increase the recovery of mRBCs frozen with polymer. Although freezing can significantly shorten the half life span of frozen cells, it can not influence the 24h recovery of frozen mRBCs. In addition, incubation with monosaccharide before freezing can not increase the life span of frozen mRBCs. So according to the above data, to increase the life span of hRBCs frozen with polymer and monosaccharide, the osmotic fragility of the frozen RBCs must be decreased in the future. PMID: 20176010 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3302910 Fri, 19 Feb 2010 00:00:00 +0100 3302910 http://www.medworm.com/index.php?rid=3284582&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20159009%26dopt%3DAbstract Authors: Pegg DE This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differenly from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs. PMID: 20159009 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3284582 Sat, 13 Feb 2010 00:00:00 +0100 3284582 http://www.medworm.com/index.php?rid=3276671&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20152822%26dopt%3DAbstract In this study, our analyses showed that antioxidants, principally catalase, performed greater preservation of: CD34+ cells, CD123+ cells, colony-forming units and cell viability, all post-thawed, compared with the standard solution of cryopreservation. Our present studies show that the addition of catalase improved the cryopreservation outcome. Catalase may act on reducing levels of ROS, further indicating that accumulation of free radicals indeed leads to death in cryopreserved hematopoietic cells. PMID: 20152822 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3276671 Tue, 09 Feb 2010 00:00:00 +0100 3276671 http://www.medworm.com/index.php?rid=3276670&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20152823%26dopt%3DAbstract Authors: Petrenko AY, Cherkashina DV, Somov AY, Tkacheva EN, Semenchenko OA, Lebedinsky AS, Fuller BJ Reversible uncoupling of the mitochondrial electron transport chain may be one strategy to prevent intracellular oxidative stress during liver cold preservation / warm reperfusion (CP/WR) injury. 2,4-Dinitrophenol (DNP) is a potent water-soluble uncoupling agent for supplementation of the hepatic CP solution. The aim of this work was to investigate the possible influence of DNP in the CP solution on the isolated rat liver state during CP/WR. Livers were subjected to CP at 4 (o)C in sucrose-phosphate based solution (SPS) for 18 h, followed by WR for 60 min in vitro. The final concentration of DNP was 100 muM. DNP presence during the CP stage led to partial ATP level decrease accompanied... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3276670 Tue, 09 Feb 2010 00:00:00 +0100 3276670 http://www.medworm.com/index.php?rid=3276669&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20152824%26dopt%3DAbstract Conclusion: Cryosurgery is a safe and effective ablative procedure for pancreatic tissue resulting in minimal complications. PMID: 20152824 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3276669 Tue, 09 Feb 2010 00:00:00 +0100 3276669 http://www.medworm.com/index.php?rid=3255029&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20138169%26dopt%3DAbstract This study shows that the new cryo-medium and CRF method provide better recoveries of MNC, HSC and total CFU than the control cryo-medium and iso-propylalcohol freezing (IPA) method. Therefore, this cryo-medium, combined with the CRF method, is valuable for optimizing cryopreservation conditions for HSC from UCB to obtain satisfactory HSC recovery. PMID: 20138169 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3255029 Wed, 03 Feb 2010 00:00:00 +0100 3255029 http://www.medworm.com/index.php?rid=3248871&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20132808%26dopt%3DAbstract Authors: Nevena G, Melita V, Mirjana M, Svetlana D, Aleksandra U, Jelena A, Goran P Although cryosurgery is attaining increasing clinical acceptance, our understanding of the mechanisms of cryogenic cell destruction remains incomplete. While it is generally accepted that cryoinjured cells die by necrosis, the involvement of apoptosis was recently shown. Our studies of liver cell death by cryogenic temperature revealed the activation of endonuclease p23 and its de novo association with the nuclear matrix. This finding is strongly suggestive of a programmed-type of cell death process. The presumed order underlying cryonecrotic cell death is addressed here by examining the mechanism of p23 activation. To that end, nuclear proteins that were prepared from fresh liver, which is devoid of p2... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3248871 Mon, 01 Feb 2010 00:00:00 +0100 3248871 http://www.medworm.com/index.php?rid=3212780&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20097191%26dopt%3DAbstract In conclusion, this study emphasizes that using strict size maintenance as the primary objective does not result in drug complete retention inside the liposome core. PMID: 20097191 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3212780 Thu, 21 Jan 2010 00:00:00 +0100 3212780 http://www.medworm.com/index.php?rid=3186240&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20079725%26dopt%3DAbstract In this study the vitrification was evaluated in order to cryopreserve the immature domestic cat oocytes by assessing the survival of cumulus-oocyte complexes (COC), and the development competence after IVM and IVF by fresh cat epididymal sperms. From a total of 892 COC obtained from queens after ovariectomy were divided into two groups: experiment 1 for viability evaluation (150 vitrified and 100 control COC) and experiment 2 for assessing the developmental competence (414 vitrified and 228 control COC).The viability was evaluated by double staining with carboxyfluorescein and Trypan-blue, while the developmental competence was evaluated by in vitro maturation (IVM), in vitro fertilization (IVF) by fresh epididymal spermatozoa and in vitro culture (IVC).The vitrification was performed in ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3186240 Wed, 13 Jan 2010 00:00:00 +0100 3186240 http://www.medworm.com/index.php?rid=3172280&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20067781%26dopt%3DAbstract Authors: Lee JK, Park KS, Park S, Park H, Song YH, Kang SH, Kim HJ A psychrophilic yeast was isolated from an Arctic pond and its culture supernatant showed ice-binding activity. This isolate, identified as Leucosporidium sp. based on an analysis of the D1/D2 and ITS regions of its ribosomal DNA, produced a secretory ice-binding protein (IBP). Yeast IBP was purified from the culture medium to near homogeneity by the ice affinity method and appeared to be glycosylated with a molecular mass of approximately 26 kDa. In addition, the yeast IBP was shown to have thermal hysteresis (TH) and recrystallization inhibition (RI) activities. The full-length cDNA for yeast IBP was determined and was found to encode a 261 amino acid protein with molecular weight of 26.8 kDa that includes an N-termin... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3172280 Sat, 09 Jan 2010 00:00:00 +0100 3172280 http://www.medworm.com/index.php?rid=3172278&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20067782%26dopt%3DAbstract This study supported that exposure to cryoprotectants prior to freezing not only reduce the freeze-thaw damage but also provide various process to the recovery from freeze-thaw damage. PMID: 20067782 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3172278 Sat, 09 Jan 2010 00:00:00 +0100 3172278 http://www.medworm.com/index.php?rid=3135766&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20040364%26dopt%3DAbstract In this study, we analyzed the supercooling-facilitating activities of 12 kinds of flavonol glycosides in order to determine the chemical structures that might affect supercooling-facilitating activity. All of the flavonol glycosides tested showed supercooling-facilitating activity, although the magnitudes of activity differed among the compounds. It was clear that the combination of the position of attachment of the glycosyl moiety, the kind of attached glycosyl moiety and the structure of aglycone determined the magnitude of anti-ice nucleation activity. However, there is still some ambiguity preventing the exact identification of features that affect the magnitude of supercooling-facilitating activity. PMID: 20040364 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3135766 Sat, 26 Dec 2009 00:00:00 +0100 3135766 http://www.medworm.com/index.php?rid=3119437&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20026102%26dopt%3DAbstract Authors: Brockbank KG, Chen ZZ, Song YC The limited availability of fresh osteochondral allograft tissues necessitates the use of banking for long-term storage. A vitrification solution containing a 55% cryoprotectant formulation, VS55, previously studied using rabbit articular cartilage, was evaluated using porcine articular cartilage. Specimens ranging from 2-6mm in thickness were obtained from 6mm distal femoral cartilage cores and cryopreserved by vitrification or freezing. The results of post-rewarming viability assessments employing alamar-Blue demonstrated a large decrease (p<0.001) in viability in all 3 sizes of cartilage specimen vitrified with VS55. This is in marked contrast with prior experience with full thickness, 0.6mm rabbit cartilage. Microscopic examination followi... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3119437 Thu, 17 Dec 2009 00:00:00 +0100 3119437 http://www.medworm.com/index.php?rid=3066368&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19962974%26dopt%3DAbstract Authors: Mazur P Thermodynamics and physical chemistry have played powerful roles the past forty-five years in interpreting cryobiological problems and in predicting cryobiological outcomes. The author has been guided by a few core principles in using these concepts and tools and this paper discusses these core principles. They are (1) the importance of chemical potentials and of the difference between the chemical potentials of water and solutes inside the cell and outside in determining the direction and rate of fluxes of water and solutes. (2) The influence of the curvature of an ice crystal on its chemical potential and on the ability of ice to pass through pores in cell membranes, on the nucleation temperature of supercooled water, and on the recrystallization of ice. (3) The use ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3066368 Thu, 03 Dec 2009 00:00:00 +0100 3066368 http://www.medworm.com/index.php?rid=3049002&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19948163%26dopt%3DAbstract Authors: Choi J, Bischof JC It is well accepted in Cryobiology that the temperature history and cooling rates experienced in biomaterials during freezing procedures correlate strongly with biological outcome. Therefore, heat transfer measurement and prediction in the cryogenic regime is central to the field. Although direct measurement of heat transfer can be performed, accuracy is usually achieved only for local measurements within a given system and cannot be readily generalized to another system without the aid of predictive models. The accuracy of these models rely upon thermal properties which are known to be highly dependent on temperature, and in the case of significant cryoprotectant loading, also on crystallized fraction. In this work we review the available thermal properties... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3049002 Fri, 27 Nov 2009 00:00:00 +0100 3049002 http://www.medworm.com/index.php?rid=3031384&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19932092%26dopt%3DAbstract Authors: Meng FY, Chen ZS, Han M, Hu XP, Zhou P A modified purification procedure is described for effectively eliminating dead cells after hepatocyte cyropreservation. Isolated hepatocytes from 6 pig tissue samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, we developed a preincubation step prior to gradient centrifugation. The hepatocytes were subsequent cultured in suspension overnight (12-16 h), and then dead cells were eliminated by Ficoll 400 purification. The results showed that a high viability (mean of 96%) of cells was obtained, with a low viable cell loss in number (2-5%), by using this modified method. PMID: 19932092 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3031384 Thu, 19 Nov 2009 00:00:00 +0100 3031384 http://www.medworm.com/index.php?rid=3009004&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19919830%26dopt%3DAbstract In conclusion, both the developmental stage of IVP ovine embryos and the method of vitrification have a significant effect on the viability and developmental competence of sheep embryos. PMID: 19919830 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=3009004 Sat, 14 Nov 2009 00:00:00 +0100 3009004 http://www.medworm.com/index.php?rid=3000614&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19913528%26dopt%3DAbstract Authors: Braulke LJ, Heldmaier G During entrance into torpor heart and respiration rates are greatly reduced in parallel with the reduction of metabolic rate, suggesting an involvement of parasympathetic control. We compared the effect of parasympathetic inhibition with the effect of sympathetic inhibition on spontaneous torpor behavior in the Djungarian hamster. Hamsters were acclimated to short photoperiod and displayed their standard torpor pattern as observed from T(b) records. Parasympathetic inhibition was achieved by a subcutaneous implant of 21-day release pellets with Atropine and the sympathetic noradrenergic pathway was inhibited with a single injection of 6-Hydroxydopamine. Atropine treatment did not affect the occurrence and quality of spontaneous daily torpor at all. Howe... Cryobiology journals http://www.medworm.com/rss/comments.php?id=3000614 Wed, 11 Nov 2009 00:00:00 +0100 3000614 http://www.medworm.com/index.php?rid=2977868&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895799%26dopt%3DAbstract Authors: Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M Cryopreservation of human semen can cause DNA damages, which compromise the fertilization and normal embryo development. The present study showed that the antioxidant resveratrol prevents these damages both in fertile and infertile men. The addition of ascorbic acid before cryopreservation can reduce DNA damages only in infertile men. Although further studies are needed, the present work showed that resveratrol could be considered in human cryopreservation procedures to avoid/minimize DNA damages and preserve sperm integrity. PMID: 19895799 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2977868 Tue, 03 Nov 2009 00:00:00 +0100 2977868 http://www.medworm.com/index.php?rid=2977867&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895800%26dopt%3DAbstract This study suggests that MpAFP698 has a high thermal stability and could be used to improve the thermal stability of the less stable proteins by producing fusion proteins, which could be used for biotechnological purposes. PMID: 19895800 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2977867 Tue, 03 Nov 2009 00:00:00 +0100 2977867 http://www.medworm.com/index.php?rid=2977866&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895801%26dopt%3DAbstract The objective of the present study is to improve the survival rate and viability of NSCs. Neural stem cells with three states of single cell suspension, NSC spheres with diameters of 30 approximately 50mum and 80 approximately 100mum, were cryopreserved by slow freezing method with the cryo-pretective agent (CPA) of dimethyl sulfoxide (Me(2)SO), respectively. Then the post-thawing NSCs were tested for the survival rate and the differentiation ability. As a result, NSC spheres with diameter of 80 approximately 100mum and Me(2)SO concentration of 8% achieve the survival rate of 82.9%, and the NSCs still sustain the multi-differentiation potentiality. These results indicated that both the subtle interaction among NSCs and sphere diameters may affect the survival rate together. PMID: 19895... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2977866 Tue, 03 Nov 2009 00:00:00 +0100 2977866 http://www.medworm.com/index.php?rid=2951387&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19879864%26dopt%3DAbstract Authors: Jiang Z, Hu X, Kretlow JD, Liu N In order to provide a suitable source of cells for lymphatic tissue engineering, the present study was designed to investigate techniques for harvesting and cryopreservation of human dermal lymphatic endothelial cells (LECs) in vitro. The LECs were isolated from children's foreskins and then cultured in endothelial growth medium-2 MV (EGM-2-MV) with 5% FBS. The second passage LECs were suspended in cryopreservation solution containing 40% FBS and 10% Me2SO in EGM-2-MV, cooled to -80 degrees C at about 1 degrees C /min and stored in liquid nitrogen. Samples were thawed quickly in a 37 degrees C water bath, and the cryoprotectant was removed by serial elution. The membrane integrity of thawed LECs was determined by trypan blue staining exclusion,... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2951387 Thu, 29 Oct 2009 00:00:00 +0100 2951387 http://www.medworm.com/index.php?rid=2935339&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19857479%26dopt%3DAbstract Authors: Taylor MJ, Baicu SC This review focuses on the application of hypothermic perfusion technology as a topic of current interest with the potential to have a salutary impact on the mounting clinical challenges to improve the quantity and quality of donor organs and the outcome of transplantation. The ex vivo perfusion of donor organs on a machine prior to transplant, as opposed to static cold storage on ice, is not a new idea but is being re-visited because of the prospects of making available more and better organs for transplantation. The rationale for pursuing perfusion technology will be discussed in relation to emerging data on clinical outcomes and economic benefits for kidney transplantation. Reference will also be made to on-going research using other organs with special ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2935339 Fri, 23 Oct 2009 00:00:00 +0100 2935339 http://www.medworm.com/index.php?rid=2935338&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19857480%26dopt%3DAbstract This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze/thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statistically significant control factors that affect the viability of the cell. Results show that the type of cryoprotectant, freezing rate, thawing rate, and concentration of cryoprotectant (listed in the order of influence) are the statistically significant control factors that affect the post-thaw viability. The end temperature and durations of the first and second stages of freezing do not affect the post-thaw viability. Within the ranges of the control factors studied in this work, ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2935338 Fri, 23 Oct 2009 00:00:00 +0100 2935338 http://www.medworm.com/index.php?rid=2935337&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19857481%26dopt%3DAbstract Authors: Nishigaki T, Teramura Y, Suemori H, Iwata H Human embryonic stem (hES) cells are expected to be useful in the fields of regenerative medicine and tissue engineering due to their pluripotency. Therefore, it is necessary to establish highly efficient and reliable methods for the cryopreservation of hES cells. We have cryopreserved cynomolgus and human ES cells by the vitrification method, using a chemically-defined dimethyl sulfoxide (Me(2)SO)-free and serum-free medium composed of Euro-Collins solution as a base medium and 40% (v/v) ethylene glycol (EG) and 10% (w/v) polyethylene glycol (PEG) as cryoprotectants. When the vitrification and the cryoprotectants were combined, the recovery ratio of hES cells was 22.9 + 7.7%, compared to 0.4 + 0.2% when the conventional slow-freezin... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2935337 Fri, 23 Oct 2009 00:00:00 +0100 2935337 http://www.medworm.com/index.php?rid=2935336&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19857482%26dopt%3DAbstract Authors: Hagedorn M, Carter VL, Leong JC, Kleinhans FW Coral throughout the world are under threat. To save coral via cryopreservation methods, the Symbiodium algae that live within many coral cells must also be considered. Coral juvenile must often take up these important cells from their surrounding water and when adult coral bleach, they lose their endosymbiotic algae and will die if they are not regained.The focus of this paper was to understand some of the cryo-physiology of the endosymbiotic algae, Symbiodinium, living within three species of Hawaiian coral, Fungia scutaria, Porites compressa and Pocillopora damicornis in Kaneohe Bay, Hawaii.Although cryopreservation of algae is common, the successful cryopreservation of these important coral endosymbionts is not common, and thes... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2935336 Fri, 23 Oct 2009 00:00:00 +0100 2935336 http://www.medworm.com/index.php?rid=2935340&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19852953%26dopt%3DAbstract Authors: Higgins AZ, Karlsson JO In a companion paper, we demonstrated that dynamic range limitations can confound measurement of the osmotically inactive volume using electrical sensing zone instruments (e.g., Coulter counters), and presented an improved parameter estimation method in which a lognormal function was fit to the cell volume distribution to allow extrapolation beyond the bounds of the data. Presently, we have investigated the effect of dynamic range limitations on measurement of the cell membrane water permeability (L(p)), and adapted the lognormal extrapolation method for estimation of L(p) from transient volume data. An alternative strategy (the volume limit adjustment method, in which the measured isotonic volume distribution is used to generate model predictions for c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2935340 Tue, 20 Oct 2009 00:00:00 +0100 2935340 http://www.medworm.com/index.php?rid=2924009&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19850023%26dopt%3DAbstract Authors: Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata KI, Watanabe M, Kikawada T, Okuda T Dry-preservation of nucleated cells from multicellular animals represents a significant challenge in life science. As anhydrobionts can tolerate a desiccated state, their cells and organs are expected to show high desiccation tolerance in vitro. In the present study, we established cell lines derived from embryonic tissues of an anhydrobiotic chironomid, Polypedilum vanderplanki, designated as Pv11 and Pv210. Salinity stress induced the expression of a set of anhydrobiosis-related genes in both Pv11 and Pv210 cells, suggesting that at least a part of cells can autonomously control the physiological changes for the entry into anhydrobiosis. When desiccated with medium supplemented with 30... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2924009 Mon, 19 Oct 2009 00:00:00 +0100 2924009 http://www.medworm.com/index.php?rid=2902045&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19833119%26dopt%3DAbstract Authors: Gage AA, Baust JM, Baust JG Cryosurgery is the use of freezing temperatures to elicit an ablative response in a targeted tissue. This review provides a global overview of experimentation in vivo which has been the basis of advancement of this widely applied therapeutic option. The cellular and tissue-related events that underlie the mechanisms of destruction, including direct cell injury (cryolysis), vascular stasis, apoptosis and necrosis, are described and are related to the optimal methods of technique of freezing to achieve efficacious therapy. In vivo experiments with major organs, including wound healing, the putative immunological response following thawing, and the use of cryoadjunctive strategies to enhance cancer cell sensitivity to freezing, are described. PMID:... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2902045 Sun, 11 Oct 2009 23:00:00 +0100 2902045 http://www.medworm.com/index.php?rid=2902044&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19833120%26dopt%3DAbstract In this study, juvenile ovaries (10-day-old) collected from genetically modified mouse lines were cryopreserved using high concentrations of cryoprotectants (dimethyl sulfoxide (Me(2)SO) and ethylene glycol (EG)) and instrumented ultra-rapid freezing. The validation of the frozen ovary batches was assessed by orthotopically transplanting a thawed ovary into a nearly completely ovariectomized mature female (congenic with the ovary donor). After 2 weeks of recovery, the ovary recipient was continuously paired with a male (congenic with the ovary donor) to evaluate the fertility of the recipient and delivered offspring were genotyped to evaluate the continued functionality of the grafted ovary. The recipient females delivered genetically modified offspring starting 6 weeks after ovary transpl... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2902044 Sun, 11 Oct 2009 23:00:00 +0100 2902044 http://www.medworm.com/index.php?rid=2887305&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818745%26dopt%3DAbstract Authors: Song Y, Sharp R, Lu F, Hassan M Cryopreservation of human gonadal tissue and oocytes has brought about new and exciting research in reproductive medicine, as well as new cryopreservation techniques that are dramatically improving post-thaw viability and freezing damage. The work done on gonadal tissues is aimed at improving the quality of life for infertile patients and for prepubertal patients undergoing gonadotoxic chemotherapy, patients for whom hormonal stimulation /IVF is not an option, and women without partners. Cryopreservation of mature oocytes is the best model for timing IVF. Vitrification is likely to benefit the field, and since 2005, implantation and pregnancy rates from vitrified oocytes have matched or eclipsed results from conventional methods, due to new cell... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2887305 Tue, 06 Oct 2009 23:00:00 +0100 2887305 http://www.medworm.com/index.php?rid=2847148&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19786008%26dopt%3DAbstract In conclusion, retrograde flushing is recommended for ibex sperm collection since it would appear that microbial contamination is no more of a problem than that encountered with the cutting method, while a larger number of sperm cells more resistant to freezing-thawing can be obtained. PMID: 19786008 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2847148 Thu, 24 Sep 2009 23:00:00 +0100 2847148 http://www.medworm.com/index.php?rid=2847147&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19786009%26dopt%3DAbstract Authors: Paredes E, Bellas J Current strategies for marine pollution monitoring are based on the integration of chemical and biological techniques. The sea urchin embryo-larval bioassays are among the biological methods most widely used world-wide. Cryopreservation of early embryos of sea urchins could provide a useful tool to overcome one of the main limitations of such bioassays, the availability of high quality biological material all year round. The present study aimed to determine the suitability of several permeant (dimethyl sulfoxide -Me(2)SO-, propylene glycol -PG- and ethylene glycol -EG-) and non permeant (trehalose -TRE-, polyvinylpirrolidone -PVP-) cryoprotectant agents (CPAs) and their combination, for the cryopreservation of eggs and embryos of the sea urchin Paracentrotu... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2847147 Thu, 24 Sep 2009 23:00:00 +0100 2847147 http://www.medworm.com/index.php?rid=2847146&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19786010%26dopt%3DAbstract Authors: Huang C, Sun C, Su X, Zhao X, Miao M, Liu Y, Dong Q In the present study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycerol as cryoprotectant, cool at 25 degrees C/min, and thaw at 40 degrees C in a water bath for 7 s. Live young were produced from females inseminated with frozen-thawed sperm in both species. In guppies, percent fertilization (F) and the number of embryos (N) produced with cryopreserved sperm (F = 50%, N = 74, from 26 females) were similar to those of fresh controls (F = 54%, N = 61, from 22 females). Intere... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2847146 Thu, 24 Sep 2009 23:00:00 +0100 2847146 http://www.medworm.com/index.php?rid=2834926&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19778531%26dopt%3DAbstract Authors: Odintsova NA, Boroda AV, Velansky PV, Kostetsky EY The development of cryopreservation methods for embryonic cells and larvae of sea animals offers a great potential for marine biotechnology. Larval cells of bivalves and sea urchins were frozen to -196 degrees C using traditional cryoprotectants (Me(2)SO and trehalose) and the cryoprotective mixture developed by us. In addition to Me(2)SO and trehalose, this mixture contained an exogenous lipid extract from mussel tissues and antioxidants. A positive effect of antioxidants (alpha-tocopherol acetate, ascorbic acid or echinochrome, the quinoid pigment of sea urchins) on cell viability became significant only in the presence of exogenous lipids. Antioxidants added to cryoprotective mixtures did not reveal visible cryoprotective a... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2834926 Sun, 20 Sep 2009 23:00:00 +0100 2834926 http://www.medworm.com/index.php?rid=2822155&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19766616%26dopt%3DAbstract Authors: Fahy GM Armand Karow, Jr. was a devoted scholar of cryobiology who was responsible for the creation of landmark books and many unique observations. Driven into cryobiology by a fascination with organ cryopreservation that dated from his high school days, Karow carried out or contributed to research on the cryopreservation of hearts, kidneys, pancreatic islets, and reproductive cells and tissues. His interests included not only conventional issues in cryobiology such as cryoprotectant permeation kinetics, theories of freezing injury in cells and tissues, and electromagnetic warming of large organs, but also more esoteric questions such as the limits of tolerance of mammalian organs to high pressures, the role of molecular hydration in cell viability, the pharmacological effects... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2822155 Wed, 16 Sep 2009 23:00:00 +0100 2822155 http://www.medworm.com/index.php?rid=2822154&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19766617%26dopt%3DAbstract Authors: Li X, Zhong Z, Liang S, Wang X, Zhong F Cryopreservation of animal or human peripheral blood mononuclear cells (PBMC) is a commonly used technique. Effects of cryopreservation on functional capacity, especially the cytokine production of human PBMCs, have been extensively defined. However, certain animals, such as livestock, are a shortage of these information. Here we investigated the effects of cryopreservation on cytokine (IL-4, IFNgamma and IL-6) production of porcine PBMC. The porcine PBMCs were cryopreserved at -196 degrees C for a variety time periods for 2, 5, 25 and 50 days. Viability and cytokine production of the porcine PBMCs were measured before and after cryopreservation. The results showed that about 90% cell recovery rate was obtained at each storage time, indi... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2822154 Wed, 16 Sep 2009 23:00:00 +0100 2822154 http://www.medworm.com/index.php?rid=2822153&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19766618%26dopt%3DAbstract Authors: Preobrazhensky SN, Bahler DW Density gradient centrifugation usually allows efficient separation of mononuclear cells from granulocytes using fresh human blood samples. However, we have found that with cryopreserved blood samples, density gradient centrifugation fails to separate granulocytes from mononuclear cells and have explored using immunomagnetic anti-CD15 microbeads as an alternate method to separate these cell populations. Using cryopreserved blood samples from 10 healthy donors we have shown that granulocytes express a significantly higher level of CD15 antigen than monocytes and lymphocytes, which allows for their efficient separation from mononuclear cells using anti-CD15 microbeads. This procedure is critical for purification of individual cell populations from cr... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2822153 Wed, 16 Sep 2009 23:00:00 +0100 2822153 http://www.medworm.com/index.php?rid=2822150&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19766619%26dopt%3DAbstract This study characterized the cryobiological responses of the human erythroleukemic stem cell line TF-1, as a model for HSC. We measured the osmotic parameters of TF-1 cells, including the osmotically inactive fraction, temperature-dependent membrane hydraulic conductivity and the membrane permeability to 1M Me2SO. A two-step freezing procedure (interrupted rapid cooling with hold time) and a graded freezing procedure (interrupted slow cooling without hold time) were used to characterize TF-1 cell recovery during various phases of the cooling process. One outcome of these experiments was high recovery of TF-1 cells cryopreserved in the absence of traditional cryoprotectants. The results of this study of the cryobiology of TF-1 cells will be critical for future understanding of the cryobiolo... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2822150 Wed, 16 Sep 2009 23:00:00 +0100 2822150 http://www.medworm.com/index.php?rid=2822156&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19766103%26dopt%3DAbstract Authors: Ferrigno A, Carlucci F, Tabucchi A, Tommassini V, Rizzo V, Richelmi P, Gringeri E, Neri D, Boncompagni E, Freitas I, Cillo U, Vairetti M We compared the susceptibility of liver grafts from lean and obese Zucker rats to preservation injury, using two organ-preservation techniques: conventional static preservation (SP) and machine perfusion (MP) preservation. SP: livers preserved by UW solution at 4, 8 or 20 degrees C for 6-hrs. MP: livers perfused for 6-hrs with an improved oxygenated Krebs-Henseleit solution (KH) at 4, 8 or 20 degrees C. Reperfusion with KH (2-hrs) was performed either with the SP or MP preserved livers. Fatty livers tolerate SP poorly at 4 degrees C, 8 degrees C and 20 degrees C as compared with MP at the same temperatures. SP induced a decrease in the ATP/AD... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2822156 Tue, 15 Sep 2009 23:00:00 +0100 2822156 http://www.medworm.com/index.php?rid=2797305&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19747475%26dopt%3DAbstract Authors: Cox SL, Hulston D, Maas EW In this research, the viability of three marine thraustochytrid isolates (fungoid protists) (WSG05, W15 and WH3) were investigated after freezing in liquid nitrogen. Five cryopreservative combinations containing horse serum, glycerol and dimethylsulfide (Me(2)SO) were used. The thraustochytrids were assessed directly after removal from liquid nitrogen and cell concentration measured for 10 days post-thawing. Results indicated that a combination of horse serum and Me(2)SO were the most effective cryoprotectants for each of the strains tested. Glycerol was only successful in producing growth in one of the strains once thawed. The protocols developed and tested in this study may have further application for cryopreserving other isolates in this class. ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2797305 Mon, 07 Sep 2009 23:00:00 +0100 2797305 http://www.medworm.com/index.php?rid=2782433&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19735650%26dopt%3DAbstract Authors: Biggar KK, Dubuc A, Storey K Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act to establish rapid biological controls that aid the reorganization of metabolic priorities for freezing survival. Selected microRNA species (miR-16 and miR-21) were evaluated using RT-PCR in liver and skeletal muscle of wood frogs (Rana sylvatica) comparing controls (5 degrees C acclimated) with animals frozen for 24 h at -3 degrees C. Levels of miR-21 increased significantly during freezing by 1.5-fold and 1.3-fold in liver and skelet... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2782433 Thu, 03 Sep 2009 23:00:00 +0100 2782433 http://www.medworm.com/index.php?rid=2775149&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19733556%26dopt%3DAbstract CONCLUSIONS: The observed effects of cold preservation and of trolox and deferiprone on the RET were comparable to those on CT muscle function, as reported in a previously published study (Van der Heijden et al, Transplantation 69 (2000); 1310-22). Both hamster RET and rat CT muscles show considerable functional damage due to actions of reactive oxygen species. In contrast to the CT, in the RET cold preservation-induced functional injury could not be prevented by BDM and was not accompanied by morphological damage such as necrosis and edema. This suggests that the RET myocytes possess a specific adaptation to withstand the Ca(2+) overload induced by cold ischemia. PMID: 19733556 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2775149 Wed, 02 Sep 2009 23:00:00 +0100 2775149 http://www.medworm.com/index.php?rid=2770474&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19729005%26dopt%3DAbstract In this study, we investigated the influence of the concentration of extracellular non-permeable and permeable solutes on the melting points of the intracellular ice in mouse oocytes using cryomicroscopy. The results showed that the melting points of the intracellular ice are always lower than the extracellular ice. Based on this observation and the Gibbs-Thomson relation, we established a physical model to calculate the size of intracellular ice crystals and described its relationship with the concentrations of intracellular permeating solutes and macromolecules. This model predicts that the increased concentration of macromolecules in cells, by increasing the extracellular non-permeating solute concentration, can significantly lower the required concentration of permeable solutes for int... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2770474 Sun, 30 Aug 2009 23:00:00 +0100 2770474 http://www.medworm.com/index.php?rid=2758898&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19723517%26dopt%3DAbstract Authors: Takeda H, Maruyama S, Okajima J, Aiba S, Komiya A We have developed a novel cryoprobe for skin cryosurgery utilizing the Peltier effect. The four most important parameters for necrotizing tissue efficiently are the cooling rate, end temperature, hold time and thawing rate. In cryosurgery for small skin diseases such as flecks or early carcinoma, it is also important to control the thickness of the frozen region precisely to prevent necrotizing healthy tissue. To satisfy these exacting conditions, we have developed a novel cryoprobe to which a Peltier module was attached. The cryoprobe makes it possible to control heat transfer to skin surface precisely using a proportional-integral-derivative (PID) controller, and because it uses the Peltier effect, the cryoprobe does not need... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2758898 Fri, 28 Aug 2009 23:00:00 +0100 2758898 http://www.medworm.com/index.php?rid=2758897&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19723518%26dopt%3DAbstract In conclusion, rewarming from deep hypothermia is not associated with increased phospholipid peroxidation. There is, however, a significant remodelling of the phospholipid fraction of myocardial lipids in vivo probably as a result of receptor or calcium stimulated phospholipase activity. Calcium or calcium stimulated phospholipase activity could contribute to posthypothermic contractile dysfunction. PMID: 19723518 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2758897 Fri, 28 Aug 2009 23:00:00 +0100 2758897 http://www.medworm.com/index.php?rid=2758896&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19723519%26dopt%3DAbstract Authors: Boryshpolets S, Dzyuba B, Rodina M, Li P, Hulak M, Gela D, Linhart O In the present study we investigated the possibility of spontaneous carp spermatozoa activation by freeze-thawing. To evaluate this, the parameters of spermatozoa motility percentage, velocity, ATP content level and fertility rate of sperm were used. The motility and velocity of spermatozoa activated by freeze-thawing were characterized by motile spermatozoa with a median value of 16% and a velocity of 98 mum/s. In addition, the motility and velocity of sperm from the thawed samples were significantly lower than in the control (median value of 100% for sperm motility and 175 mum/s for sperm velocity). Furthermore, a spontaneously activated spermatozoa motility terminated within five minutes post-thaw time. Af... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2758896 Fri, 28 Aug 2009 23:00:00 +0100 2758896 http://www.medworm.com/index.php?rid=2752275&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19716813%26dopt%3DAbstract In conclusion, in our culture conditions, the blastocyst yield is not influenced by culture system, while the cryotolerance of IVP-blastocysts is positively influenced by the presence of somatic cells. Moreover, the expanded blastocysts are more susceptible to cryoinjury than early blastocysts. PMID: 19716813 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2752275 Wed, 26 Aug 2009 23:00:00 +0100 2752275 http://www.medworm.com/index.php?rid=2735046&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19703437%26dopt%3DAbstract Authors: Mao H, Wang Y, Yuan W, Wong LB To determine air-liquid interface (ALI) culture derived from cryopreserved mammalian tracheal ciliated cells is a viable ciliated cell model for the investigations of regulatory mechanisms of ciliary beat frequency (CBF), two studies were performed using ovine and porcine tracheae obtained from local slaughterhouses. The protease-digested tracheal ciliated cells were harvested and cultured at the ALI using collagen-coated, porous membrane inserts. In study 1, the ALI culturing protocols were established using non-cryopreserved ovine tracheal ciliated cells. Ciliogenesis was documented with immuno-histology and electron micrographs. Vigorous beating cilia were video-recorded. CBF was measured by laser light scattering. The functional integrity of ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2735046 Thu, 20 Aug 2009 23:00:00 +0100 2735046 http://www.medworm.com/index.php?rid=2712899&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19686717%26dopt%3DAbstract Authors: Dong Q, Hill D, Vandevoort CA The present study evaluated the interactions among pre-cooling, cryoprotectant, cooling, and thawing for rhesus monkey sperm using a four-way factorial design. Specifically, pre-cooling and thawing were evaluated for two conditions: slow vs. fast. Cooling was evaluated at four rates of 5, 29, 200, and 400 degrees C/min. The types of cryoprotectant involved combinations of egg yolk and glycerol, egg yolk and ethylene glycol, and egg yolk alone without permeable cryoprotectants or buffer alone with glycerol but without egg yolk. Our findings showed strong interactions among cryoprotectants, cooling, and thawing rates, but not pre-cooling rate, on post-thaw motility and forward progression. The optimal combination of cooling and thawing for maximum p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2712899 Thu, 13 Aug 2009 23:00:00 +0100 2712899 http://www.medworm.com/index.php?rid=2696697&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19665010%26dopt%3DAbstract Authors: Prickett RC, Elliott JA, McGann LE The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do not take into account the interactions between different types of solute molecules or (ii) require fitting to multisolute data to obtain empirical parameters. The osmotic virial coefficients, which are obtained from single-solute data, can be used to make predictions of multisolute solution osmolality. The osmotic virial coefficients for a range of solutes of interest in cryobiology are provided in this paper, for use with concentr... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2696697 Tue, 04 Aug 2009 23:00:00 +0100 2696697 http://www.medworm.com/index.php?rid=2696696&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19665011%26dopt%3DAbstract In conclusion, the injuries caused by trehalose loading may directly lead to postthaw hemolysis and poor quality of RBCs. However, monosaccharide can enhance the recovery of frozen RBCs. The cryoprotective effect of galactose may be better than that of glucose or fructose. In the future, we will continue to look for a safe and efficient trehalose loading process and try to decrease the osmotic fragility of RBCs frozen with polymers and sugars. PMID: 19665011 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2696696 Tue, 04 Aug 2009 23:00:00 +0100 2696696 http://www.medworm.com/index.php?rid=2668370&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19646981%26dopt%3DAbstract Authors: Endoh K, Kasuga J, Arakawa K, Ito T, Fujikawa S The freezing behavior of dormant buds in larch, especially at the cellular level, was examined by a Cryo-SEM. The dormant buds exhibited typical extraorgan freezing. Extracellular ice crystals accumulated only in basal areas of scales and beneath crown tissues, areas in which only these living cells had thick walls unlike other tissue cells. By slow cooling (5 degrees C/day) of dormant buds to -50 degrees C, all living cells in bud tissues exhibited distinct shrinkage without intracellular ice formation detectable by Cryo-SEM. However, the recrystallization experiment of these slowly cooled tissue cells, which was done by further freezing of slowly cooled buds with LN and then rewarming to -20 degrees C, confirmed that some of th... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2668370 Tue, 28 Jul 2009 23:00:00 +0100 2668370 http://www.medworm.com/index.php?rid=2668372&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19646431%26dopt%3DAbstract In this study we investigate the effect of TRAIL as a sensitising agent to enhance the effects of cryotherapy on prostate cancer cells.Prostate cancer cells were cooled using Endocare cryo-system to mimic temperatures achieved during clinical cryotherapy. The effects of TRAIL, cryotherapy or combination of both treatment on DU-145 and PC-3 were evaluated. Viability and mode of cell death was assessed following treatment.Cryotherapy did not result in complete cell death at temperature -40 degrees C. Cells died by both necrosis and apoptosis. Cells which survived freeze-thaw cycle became more sensitive to a second freezing injury. TRAIL resulted in minimal cell death. Concomitant treatment of the tumour cells with TRAIL and cryotherapy resulted in complete loss of viability at -10 and -20 de... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2668372 Mon, 27 Jul 2009 23:00:00 +0100 2668372 http://www.medworm.com/index.php?rid=2668371&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19646432%26dopt%3DAbstract The objectives of this study were to determine if adding cholesterol to stallion sperm membranes alters the osmotic tolerance limits and membrane permeability characteristics of the spermatozoa. In experiment one, stallion spermatozoa were treated with cholesterol-loaded cyclodextrin (CLC), subjected to anisotonic solutions and spermatozoal motility analyzed. The spermatozoa were then returned to isotonic conditions and the percentages of motile spermatozoa again determined. CLC treatment increased the osmotic tolerance limit of stallion spermatozoa in anisotonic solutions and when returned to isotonic conditions. The second and third experiments utilized an electronic particle counter to determine the plasma membrane characteristics of stallion spermatozoa. In experiment two, stallion spe... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2668371 Mon, 27 Jul 2009 23:00:00 +0100 2668371 http://www.medworm.com/index.php?rid=2645825&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631202%26dopt%3DAbstract Authors: Seita Y, Okuda Y, Kato M, Kawakami Y, Inomata T, Ito J, Kashiwazaki N Embryo cryopreservation is a valuable tool for efficient production of animals as well as banking of genetic resources. Even though the laboratory rat is one of the most important experimental animals for various research fields, it has been reported that survival and developmental ability of cryopreserved rat embryos are generally low, especially at the early stages. The aim of the present study was to establish rapid cooling method that can be applied for cryopreservation of rat pronuclear-stage embryos using Cryotops (a device). First, optimal equilibration time was examined. Pronuclear-stage embryos were equilibrated in 7.5% ethylene glycol (EG) + 7.5% dimethylsulfoxide (DMSO) + 20% fetal calf serum (FCS... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2645825 Mon, 20 Jul 2009 23:00:00 +0100 2645825 http://www.medworm.com/index.php?rid=2627219&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19619520%26dopt%3DAbstract Authors: Elliott JA This brief manuscript introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. PMID: 19619520 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2627219 Thu, 16 Jul 2009 23:00:00 +0100 2627219 http://www.medworm.com/index.php?rid=2627221&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19616532%26dopt%3DAbstract Authors: Dias CL, Ala-Nissila T, Wong-Ekkabut J, Vattulainen I, Grant M, Karttunen M The hydrophobic effect is considered the main driving force for protein folding and plays an important role in the stability of those biomolecules. Cold denaturation, where the native state of the protein loses its stability upon cooling, is also attributed to this effect. It is therefore not surprising that a lot of effort has been spent in understanding this phenomenon. Despite these efforts, many unresolved fundamental aspects remain. In this paper we review and summarize the thermodynamics of proteins, the hydrophobic effect and cold denaturation. We start by accounting for these phenomena macroscopically then move to their atomic-level description. We hope this review will help the reader gain ins... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2627221 Wed, 15 Jul 2009 23:00:00 +0100 2627221 http://www.medworm.com/index.php?rid=2627220&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19616533%26dopt%3DAbstract In conclusion, it is possible to cryopreserve preantral follicles from pig ovarian tissue using Me2SO or EG. PMID: 19616533 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2627220 Wed, 15 Jul 2009 23:00:00 +0100 2627220 http://www.medworm.com/index.php?rid=2627222&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19615991%26dopt%3DAbstract Authors: Karlsson JO Predictions by various mathematical models of intracellular ice formation (proposed by Mazur, Pitt, Toner, and Karlsson, respectively) were compared to the known thermodynamic and kinetic behavior of ice formation in supercooled aqueous systems. The older models (Mazur, Pitt, and Toner) significantly underestimated the magnitude of colligative nonequilibrium freezing point depression in response to increased concentration of solutes, such as salts or cryoprotectants. Furthermore, kinetics predicted using phenomenological models (by Mazur and Pitt) exhibited implausible temperature-dependence, with the probability of intracellular ice formation being allowed to increase even at temperatures below the glass transition point. The Toner model, on the other hand, produc... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2627222 Tue, 14 Jul 2009 23:00:00 +0100 2627222 http://www.medworm.com/index.php?rid=2602685&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19595995%26dopt%3DAbstract In this study the effect of cryoprotectants that have been shown to be the least toxic to zebrafish ovarian follicles (methanol and Me(2)SO), on mitochondria of stage III ovarian follicles was evaluated. The mitochondrial distributional arrangement, mitochondrial membrane potential, mtDNA copy number, ATP levels and ADP/ATP ratios were assessed following exposure to cryoprotectants for 30 min at room temperature. Results obtained by confocal microscopy showed that 30 min exposure to 2M methanol induced a loss of membrane potential, although viability tests showed no decrease in survival even after 5 h post-exposure incubation. Higher concentrations of methanol (3M and 4M) induced not only a decrease in mitochondrial membrane potential but also the loss of mitochondrial distributional arran... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2602685 Thu, 09 Jul 2009 23:00:00 +0100 2602685 http://www.medworm.com/index.php?rid=2602684&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19596315%26dopt%3DAbstract The objective of the present study was to investigate the potential application of intra- and extracellular raffinose in combination with low concentrations of dimethylsulfoxide (Me(2)SO) to mammalian oocyte cryopreservation. To this end, the fertilization and embryonic development of cryopreserved metaphase II (M II) mouse oocytes were studied in comparison to unfrozen controls. For cryopreservation, M II oocytes were microinjected with 0.1 M raffinose, and then cooled to -196 degrees C in the presence of either 0.3 M raffinose and 0.5 M Me(2)SO (cryopreservation group 1) or 0.3 M raffinose and 1.0 M Me(2)SO (cryopreservation group 2). The control groups included untreated oocytes (untreated control) and oocytes microinjected with raffinose, but not frozen (injection control). The post-th... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2602684 Tue, 07 Jul 2009 23:00:00 +0100 2602684 http://www.medworm.com/index.php?rid=2602686&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19591817%26dopt%3DAbstract This study uses two-photon laser scanning confocal microscopy (LSCM) to evaluate the effect of cryopreservation on collagen and elastin integrity within the leaflet and conduit of aortic and pulmonary human heart valves. To permit pairwise comparisons of fresh and cryopreserved tissue, tests valves were bisected longitudinally with one segment imaged fresh and the other imaged after cryopreservation and brief storage in liquid nitrogen. Collagen was detected by second harmonic generation (SHG) stimulation and elastin autofluorescence excitation. Qualitative analysis of all resultant images indicated the maintenance of collagen and elastin structure within leaflet and conduit post-cryopreservation. Analysis of the optimized percent laser transmission (OPLT) required for full dynamic range i... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2602686 Mon, 06 Jul 2009 23:00:00 +0100 2602686 http://www.medworm.com/index.php?rid=2577752&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19576196%26dopt%3DAbstract In this study, we examined the feasibility of cryopreservation of tissue-engineered bone (TEB) composed of osteo-induced canine bone marrow mesenchymal stem cells (cBMSCs) and partially demineralized bone matrix (pDBM) scaffold by vitrification. A novel vitreous solution named as VS442 containing 40% dimethyl-sulfoxide (DMSO), 40% EuroCollins (EC) solution and 20% basic culture medium (BCM) was developed. After being cultured in vitro for 8 days, cell/scaffold complex in VS442 was subjected to vitreous preservation for 7 days and 3 months, respectively. Cell viability, proliferation and osteogenic differentiation of cBMSCs in TEB after vitreous cryopreservation were examined with parallel comparisons being made with those cryopreserved in VS55 vitreous solution. Compared with that cryopres... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2577752 Mon, 29 Jun 2009 23:00:00 +0100 2577752 http://www.medworm.com/index.php?rid=2556723&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19559692%26dopt%3DAbstract Authors: Preciado JA, Rubinsky B Isochoric (constant volume) preservation is an alternative to traditional cryopreservation methods because it requires less cryoprotectant and is simple to operate. In order to validate that this method automatically minimizes the pressure for a given temperature, pressure and temperature data were collected from a specially designed pressure vessel. This vessel was then used to examine the effect of an isochoric environment on freezing point nucleation in an aqueous antifreeze protein solution, and to generate pressure-temperature phase diagrams for various cryoprotectant solutions. Our results show that the isochoric pressure vessel follows the pressure-temperature phase diagram of water, thereby minimizing the pressure for the given temperature. We a... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2556723 Tue, 23 Jun 2009 23:00:00 +0100 2556723 http://www.medworm.com/index.php?rid=2547406&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19545558%26dopt%3DAbstract Authors: Alapati R, Stout M, Saenz J, Gentry Jr GT, Godke RA, Devireddy RV There are very few experimental reports on the comparative water transport (membrane permeability) characteristics of ejaculated and epididymal mammalian spermatozoa during freezing. In the present study, we report the effects of cooling ejaculated and epididymal bovine sperm from the same males with and without the presence of a cryoprotective agent, glycerol. Water transport data during freezing of ejaculated and epididymal bovine sperm suspensions were obtained at a cooling rate of 20 C/min under two different conditions: 1) in the absence of any cryoprotective agents, CPAs and, 2) in the presence of 0.7 M glycerol. Using values published in the literature, we modeled the spermatozoa as a cylinder of length 3... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547406 Thu, 18 Jun 2009 23:00:00 +0100 2547406 http://www.medworm.com/index.php?rid=2547407&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19540217%26dopt%3DAbstract Authors: Macdonald JA, Degenhardt T, W Baynes J, Storey KB The effects of in vivo freezing and glucose cryoprotectant on protein glycation were investigated in the wood frog, Rana sylvatica. Our studies revealed no difference in the fructoselysine content of blood plasma sampled from control, 27 h frozen and 18 h thawed wood frogs. Glycated hemoglobin (GHb) decreased slightly with 48 h freezing exposure and was below control levels after 7 d recovery, while glycated serum albumin was unchanged by 48 h freezing but did increase after 7 d of recovery. In vitro exposure of blood lysates to glucose revealed that the GHb production in wood frogs was similar to that of the rat but was lower than in leopard frogs. We conclude that wood frog hemoglobin was glycated in vitro; however, GHb produ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547407 Tue, 16 Jun 2009 23:00:00 +0100 2547407 http://www.medworm.com/index.php?rid=2547408&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19539618%26dopt%3DAbstract We present the revealed phenomenon of cluster cyrystallization of these solutions as well as principles of describing this phenomenon using state diagrams, containing the intervals of concentration corresponding to the existence of amorphous and cryocolloid fractions. We note that for the cryocolloid fraction, a low temperature association of molecules of cryoprotective agents leads the formation of ice nanocrystals either close to or directly inside the aggregations. These fractions exist in cooled cryoprotective solutions near the vitrification temperatures of the liquid phase and may contribute to the initiation of damaging events in cryopreserved biological systems. Our data may be helpful in explaining the peculiarities observed during crystallization of cryoprotective solutions and m... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547408 Mon, 15 Jun 2009 23:00:00 +0100 2547408 http://www.medworm.com/index.php?rid=2547413&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19538951%26dopt%3DAbstract This article reviews the current status of static hypothermic storage methods and discusses potential avenues for future exploitation of this technology as the available organ pool is expanded into the more marginal donor categories. PMID: 19538951 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547413 Sun, 14 Jun 2009 23:00:00 +0100 2547413 http://www.medworm.com/index.php?rid=2547412&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19538952%26dopt%3DAbstract Authors: Powell DB, Palm Jr RC, Mackenzie AP, Winton JR The effects of temperature, ionic strength, and new cryopreservatives derived from polar ice bacteria were investigated to help accelerate the development of economical, live attenuated vaccines for aquaculture. Extracts of the extremophile Gelidibacter algens functioned very well as part of a lyophilization cryoprotectant formulation in a 15-week storage trial. The bacterial extract and trehalose additives resulted in significantly higher colony counts of columnaris bacteria (Flavobacterium columnare) compared to nonfat milk or physiological saline at all time points measured. The bacterial extract combined with trehalose appeared to enhance the relative efficiency of recovery and growth potential of columnaris in flask culture c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547412 Sun, 14 Jun 2009 23:00:00 +0100 2547412 http://www.medworm.com/index.php?rid=2547411&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19538953%26dopt%3DAbstract This study investigated additional processing and cryobiological characteristics of DPSC, ending with development of procedures for banking. First, we aimed to optimize cryopreservation of established DPSC cultures, with regards to optimizing the cryoprotective agent (CPA), the CPA concentration, the concentration of cells frozen, and storage temperatures. Secondly, we focused on determining cryopreservation characteristics of enzymatically digested tissue as a cell suspension. Lastly, we evaluated the growth, surface markers and differentiation properties of DPSC obtained from intact teeth and undigested, whole dental tissue frozen and thawed using the optimized procedures. In these experiments it was determined that Me(2)SO at a concentration between 1 and 1.5M was the ideal cryopreserva... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547411 Sun, 14 Jun 2009 23:00:00 +0100 2547411 http://www.medworm.com/index.php?rid=2547410&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19538954%26dopt%3DAbstract Authors: Zhang S, Fan J, Xu X, Chen G, Zhu F, Yan L Trehalose is widely used as a freeze-drying protectant in biomaterial preservation. For this purpose, trehalose has to be loaded into the cells but this is difficult and many methods have been tried. The application of ultrasound can temporarily permeabilize cell membranes, which offers a nonchemical, nonviral, and noninvasive method of cellular drug delivery. Ultrasound is employed here to enhance the loading of trehalose into human platelets. Two frequencies were used, 25 kHz and 800 kHz. The estimated intensity of ultrasound in the sample was varied from 0 to 1.5 W/cm(2). The trehalose concentration in the platelets was 11.27+/-2.53 mmol/L when Wolkers et al.'s method was used without ultrasound. The application of 0.8 W/cm(2), 800... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547410 Sun, 14 Jun 2009 23:00:00 +0100 2547410 http://www.medworm.com/index.php?rid=2547409&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19538955%26dopt%3DAbstract Authors: Wowk B Vitrification is a process in which a liquid begins to behave as a solid during cooling without any substantial change in molecular arrangement or thermodynamic state variables. The physical phenomenon of vitrification is relevant to both cryopreservation by freezing, in which cells survive in glass between ice crystals, and cryopreservation by vitrification in which a whole sample is vitrified. The change from liquid to solid behavior is called the glass transition. It is coincident with liquid viscosity reaching 10(13) Poise during cooling, which corresponds to a shear stress relaxation time of several minutes. The glass transition can be understood on a molecular level as a loss of rotational and translational degrees of freedom over a particular measurement timescal... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547409 Sun, 14 Jun 2009 23:00:00 +0100 2547409 http://www.medworm.com/index.php?rid=2547414&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19527701%26dopt%3DAbstract The objectives of the current study were to determine cryobiological properties of rabbit oocytes, including the isotonic volume, osmotically inactive cell fraction (V(b)), hydraulic conductivity (L(p)), permeability (P(s)) to dimethylsulfoxide (Me(2)SO), ethylene glycol (EG), and glycerol (GLY) and to examine the correlation between cell volume excursions and viability. This has led to the development of the accumulative osmotic damage (AOD) model associated with the processes of CPA addition/dilution. Mature rabbit oocytes were perfused with 15% (V/V) CPA medium (dissolved in 1X PBS). The osmotic responses of the oocytes were videotaped. A two-parameter model was fit to the experimental data to determine the values of L(p) and P(s). Oocyte volumes reached upon equilibration with 285, 600... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547414 Thu, 11 Jun 2009 23:00:00 +0100 2547414 http://www.medworm.com/index.php?rid=2547415&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19508865%26dopt%3DAbstract Authors: Peñaranda DS, Pérez L, Gallego V, Jover M, Asturiano JF Sperm production has been obtained from European and Japanese eels, but its quality and quantity tend to be changeable. So, its cryopreservation has been tried in both species. Dimethyl sulfoxide (Me(2)SO) is the best cryoprotectant for European eel sperm, but increases the medium osmolality, inducing the activation of spermatozoa motility. To avoid this, different combinations of pH (6.5 and 8.5) and NaHCO(3) concentrations (20, 40 and 80mM) were tested with two Me(2)SO concentrations (5% and 10%). Foetal bovine serum (FBS, 25%v/v) was added as a membrane protector to all the freezing media used in the different experiments. The highest Me(2)SO and NaHCO(3) concentrations at pH 6.5 caused the best post-thawing ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547415 Sat, 06 Jun 2009 23:00:00 +0100 2547415 http://www.medworm.com/index.php?rid=2547416&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19501081%26dopt%3DAbstract Authors: Fahy GM Cryoprotectant toxicity is a fundamental limiting factor for the successful cryopreservation of living systems by both freezing and vitrification, and the ability to negate it would be attractive. Past attempts to demonstrate "cryoprotectant toxicity neutralization" (CTN) have had many ups and downs. First convincingly introduced by Baxter and Lathe in 1971, the concept that certain amides can block toxic effects of dimethyl sulfoxide (Me(2)SO) was contradicted by direct experiments in 1990. But in 1995, the opposite mode of CTN, in which Me(2)SO blocked the damaging effects of formamide, was robustly demonstrated. Recent experiments have verified the original 1995 results and extended them to urea and acetamide, but no CTN was detected for N-methylamides (N-methylform... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547416 Fri, 05 Jun 2009 23:00:00 +0100 2547416 http://www.medworm.com/index.php?rid=2547417&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19501080%26dopt%3DAbstract Authors: Wowk B Vitrification is a process in which liquids cooled to a low temperature begin to behave as a solid without any substantial change in molecular arrangement or thermodynamic state variables. The physical phenomenon of vitrification is relevant to both cryopreservation by freezing, in which cells survive in glass between ice crystals, and cryopreservation by vitrification in which a whole sample is vitrified. The change from liquid to solid behavior is called the glass transition. It is coincident with liquid viscosity reaching 10(13) Poise during cooling, which corresponds to a shear stress relaxation time of several minutes. The glass transition can be understood on a molecular level as a loss of rotational and translational degrees of freedom on laboratory timescales, l... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547417 Tue, 02 Jun 2009 23:00:00 +0100 2547417 http://www.medworm.com/index.php?rid=2547428&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444965%26dopt%3DAbstract Authors: Voituron Y, Barré H, Ramløv H, Douady CJ Despite numerous mechanistic studies on physiological responses supporting freeze tolerance in anurans, few have addressed the evolutionary significance of this trait. We thus investigated the phylogenetic relationships among anuran species whose freeze tolerance has been assessed and in combination with new data on freezing tolerance of two closely related species of the European brown frogs (Rana temporaria and Rana dalmatina). The species we studied exhibited short survival times in frozen state (around 8 h for both species). Phylogenetic analysis suggests that freeze tolerance evolved at least two times among Ranidae and one or two times among Hylidae and never in Bufonidae. Furthermore, in order to assess the timing of di... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547428 Sun, 31 May 2009 23:00:00 +0100 2547428 http://www.medworm.com/index.php?rid=2547427&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444967%26dopt%3DAbstract CONCLUSIONS: These findings suggest that CO-RM could be utilized as adjuvant therapeutics in UW solutions to limit the injury sustained by donor livers during cold storage prior to transplantation, as has been similarly proposed for the heart, and kidney. PMID: 19444967 [PubMed - in process] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547427 Sun, 31 May 2009 23:00:00 +0100 2547427 http://www.medworm.com/index.php?rid=2547426&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444970%26dopt%3DAbstract Authors: Möhlmann DT It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thin films of undercooled liquid interfacial (ULI) water on surfaces of micrometer sized and larger mineral particles and microbes in icy environments far below the melting point temperature. This liquid interfacial water can be described in terms of a freezing point depression, which is due to the interfacial pressure of van der Waals forces. The physics behind the possibly also life supporting capability of nanometric films of undercooled liquid in... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547426 Sun, 31 May 2009 23:00:00 +0100 2547426 http://www.medworm.com/index.php?rid=2547425&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444971%26dopt%3DAbstract Authors: Carlsen CU, Kurtmann L, Brüggemann DA, Hoff S, Risbo J, Skibsted LH Incorporation of the fluorescent probe C11-BODIPY(581/591) in two dried membrane systems, soy bean phosphatidylcholine liposomes freeze-dried in a carbohydrate/protein matrix and Lactobacillus acidophilus (La-5) freeze-dried in a carbohydrate matrix, was successful and could be visualised by Confocal Laser Scanning Microscopy (CLSM). The C11-BODIPY(581/591) probe is a lipid oxidation reporter molecule, which is known to associate with the lipids of biological membranes and exhibit a fluorescence shift from the red range to the green range of the visible spectrum when it is oxidised together with the lipids. The present study is the first to demonstrate that the C11-BODIPY(581/591) probe can be used in dri... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547425 Sun, 31 May 2009 23:00:00 +0100 2547425 http://www.medworm.com/index.php?rid=2547424&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444972%26dopt%3DAbstract Cold-regulated proteins with potent antifreeze and cryoprotective activities in spruces (Picea spp.). Cryobiology. 2009 Jun;58(3):268-74 Authors: Jarzabek M, Pukacki PM, Nuc K Antifreeze proteins (AFPs) were obtained from intercellular spaces of spruce needles Picea abies (L.) Karst. and Picea pungens Engelm. by vacuum infiltration with ascorbic acid, followed by centrifugation to recover the infiltrate. As shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS- PAGE), apoplastic proteins are accumulated in these spruce species as a group of 5-9 polypeptide bands. These proteins have a molecular mass of 7-80 kDa. The spruce AFPs have the ability to modify the growth of ice and thermal hysteresis, TH, caused by these AFPs was close to 2.0 degrees C at a concent... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547424 Sun, 31 May 2009 23:00:00 +0100 2547424 http://www.medworm.com/index.php?rid=2547423&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19444973%26dopt%3DAbstract In conclusion, repeated WS and WBC treatments for healthy females do not lead to disorders related to altered secretions of the growth hormone, prolactin, thyrotropin, or thyroid hormones. PMID: 19444973 [PubMed - in process] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547423 Sun, 31 May 2009 23:00:00 +0100 2547423 http://www.medworm.com/index.php?rid=2547418&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19481072%26dopt%3DAbstract Authors: Turov VV, Kerus SV, Gun'ko VM The behaviour of intracellular water affected by organic solvents of different polarity in partially dehydrated marrow cells obtained from tubular bones of broiler chickens was studied using (1)H NMR spectroscopy at 210-290K. The (1)H NMR spectra of intracellular water include two signals which can be assigned to strongly (SAW, chemical shift of the proton resonance delta(H)=4-5ppm) and weakly (WAW, delta(H)=1.2-1.7ppm) associated waters which can be also divided into weakly (WBW, frozen at 250<T<273K and changes in the Gibbs free energy DeltaG>-0.8kJ/mol) and strongly (SBW, unfrozen at T<250K, DeltaG<-0.8kJ/mol) bound intracellular waters. Solvents of different polarity such as dimethylsulfoxide-d(6) (Me(2)SO-d(6)), acetonitrile-d(... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547418 Tue, 26 May 2009 23:00:00 +0100 2547418 http://www.medworm.com/index.php?rid=2547419&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19463808%26dopt%3DAbstract Authors: Quintana AB, Coda Zabetta CD, Baumgartner NO, Biancardi ME, Bessone V, Rodriguez JV, Mamprin ME, Furno G, Guibert EE, Sujatovich V Cryopreserved human cardiac valve allografts could suffer lethal damages if the temperature is elevated during cryostorage. This work describes the functional and morphological alterations suffered by human cardiac valve allografts after a gradual increment of the cryostorage temperature from -147 degrees C to -47 degrees C due to a technical failure. Three experimental groups of human pulmonary and aortic allografts were compared: fresh, cryopreserved (-147 degrees C) and cryopreserved with temperature changes from -147 degrees C up to -47 degrees C and back to -147 degrees C. Fibroblast functionality was studied to asses the degree of valvular da... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547419 Tue, 19 May 2009 23:00:00 +0100 2547419 http://www.medworm.com/index.php?rid=2547421&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19460363%26dopt%3DAbstract Authors: Devireddy RV An overview of the major issues involved in the statistical thermodynamic treatment of phospholipid membranes at the atomistic level is summarized: thermodynamic ensembles, initial configuration (or the physical system being modeled), force field representation as well as the representation of long-range interactions. This is followed by a description of the various ways that the simulated ensembles can be analyzed: area of the lipid, mass density profiles, radial distribution functions (RDFs), water orientation profile, deuterium order parameter, free energy profiles and void (pore) formation; with particular focus on the results obtained from our recent molecular dynamic (MD) simulations of phospholipids interacting with dimethylsulfoxide (Me(2)SO), a commonly u... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547421 Mon, 18 May 2009 23:00:00 +0100 2547421 http://www.medworm.com/index.php?rid=2547420&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19460364%26dopt%3DAbstract Authors: Luciano AM, Chigioni S, Lodde V, Franciosi F, Luvoni GC, Modina SC Oocyte cryopreservation in carnivores can significantly improve assisted reproductive technologies in animal breeding and preservation programs for endangered species. However, the cooling process severely affects the integrity and the survival of the oocyte after thawing and may irreversibly compromise its subsequent developmental capability. In the present study, two different methods of oocyte cryopreservation, slow freezing and vitrification, were evaluated in order to assess which of them proved more suitable for preserving the functional coupling with cumulus cells as well as nuclear and cytoplasmic competence after warming of immature feline oocytes. From a total of 422 cumulus enclosed oocytes (COCs) ob... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547420 Mon, 18 May 2009 23:00:00 +0100 2547420 http://www.medworm.com/index.php?rid=2547422&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19454281%26dopt%3DAbstract Authors: Wang HY, Inada T, Funakoshi K, Lu SS Control of ice formation is crucial in cryopreservation of biological substances. Successful vitrification using several additives that inhibit ice nucleation in vitrification solutions has previously been reported. Among these additives, here we focused on a synthetic polymer, poly(vinyl alcohol) (PVA), and investigated the effects of PVA on nucleation and growth of ice in 35% (w/w) aqueous 1,2-propanediol solution by using a differential scanning calorimetry (DSC) system equipped with a cryomicroscope. First, the freezing temperature of the solution was measured using the DSC system, and then the change in ice fraction in the solution during cooling was evaluated based on images obtained using the cryomicroscope, at different concentratio... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547422 Sun, 17 May 2009 23:00:00 +0100 2547422 http://www.medworm.com/index.php?rid=2547429&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19427303%26dopt%3DAbstract Authors: Seki S, Mazur P The formation of more than trace amounts of ice in cells is lethal. The two contrasting routes to avoiding it are slow equilibrium freezing and vitrification. The cryopreservation of mammalian oocytes by either method continues to be difficult, but there seems a slowly emerging consensus that vitrification procedures are somewhat better for mouse and human oocytes. The approach in these latter procedures is to load cells with high concentrations of glass-inducing solutes and cool them at rates high enough to induce the glassy state. Several devices have been developed to achieve very high cooling rates. Our study has been concerned with the relative influences of warming rate and cooling rate on the survival of mouse oocytes subjected to a vitrification procedu... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547429 Wed, 06 May 2009 23:00:00 +0100 2547429 http://www.medworm.com/index.php?rid=2547432&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19426723%26dopt%3DAbstract Authors: Li LF, Yue H, Ma J, Guan WJ, Ma YH A fibroblast line (named SCF36) from ear marginal tissue of Simmental cattle was established successfully by direct culture of explants and cell cryopreservation techniques. Biological analysis showed that the population doubling time of the thawed cells was 42.8h. The average viability of the cells was 96.8% before freezing and 91.5% after thawing. Measurements of lactic dehydrogenase and malic dehydrogenase isoenzymes showed no cross-contamination of this cell line with other species. Karyotyping showed that the frequency of cells with chromosome number 2n=60 was more than 90%. Tests for bacteria, fungi, viruses and mycoplasmas were negative. The efficiencies of expression of enhanced green, yellow and red fluorescent protein genes (pEGFP-N... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547432 Tue, 05 May 2009 23:00:00 +0100 2547432 http://www.medworm.com/index.php?rid=2547431&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19426724%26dopt%3DAbstract This study documented the extent of individual animal variation with respect to two proven methods of sperm cryopreservation in a captive population of 22 koalas. Semen samples were collected by electroejaculation, diluted in Tris-citrate glucose and equilibrated to 4 degrees C before being further diluted and frozen in media containing a final concentration of either 14% (v/v) glycerol or 12.5% (v/v) dimethylacetamide (DMA). There were significant differences in post-thaw survival of spermatozoa from different animals that were independent of pre-freeze semen quality. Glycerol proved to be a better cryoprotectant than DMA in terms of maintaining motility, plasma membrane integrity and high mitochondrial membrane potential; however, there was no difference between cryoprotectants with rega... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547431 Tue, 05 May 2009 23:00:00 +0100 2547431 http://www.medworm.com/index.php?rid=2547430&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19426725%26dopt%3DAbstract Authors: Bassukas ID, Gaitanis G PMID: 19426725 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547430 Tue, 05 May 2009 23:00:00 +0100 2547430 http://www.medworm.com/index.php?rid=2547433&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19422816%26dopt%3DAbstract Authors: Hu JH, Li QW, Zhang T, Jiang ZL Gynostemma Pentaphyllum Polysaccharide (GPP) was added at concentrations of 0.25, 0.5, 1.0, 1.5 and 2.0mg/ml to the extenders used to freeze boar semen and its effects on the quality of frozen-thawed sperm were assessed. The sperm motility was significantly higher in the extenders containing 0.25 and 0.5mg/ml GPP, as compared to other groups (P<0.05). The extender supplemented with 0.5mg/ml GPP favored the highest intact membrane and intact acrosome percentages in comparison with other groups (P<0.05), respectively. The mitochondrial activity was significantly higher at the concentrations of 0.25, 0.5 and 1.0mg/ml GPP than that of other treatments, and the control group (P<0.05). In biochemical assays, the extender supplemented with 0.2... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547433 Mon, 04 May 2009 23:00:00 +0100 2547433 http://www.medworm.com/index.php?rid=2547434&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19397904%26dopt%3DAbstract Authors: Lin C, Spikings E, Zhang T, Rawson DM Cryopreservation is now common practice in the fields of aquaculture, conservation and biomedicine. However, there is a lack of information on the effect of chilling and cryopreservation at the molecular level. In the present study, we used real-time RT-PCR analysis to determine the effect of chilling and cryopreservation on expression of Pax2a, Pax2b, Pax5 and Pax8 which constitute one subgroup of the Pax gene family. As intact embryos of zebrafish have not yet been successfully cryopreserved, we have used two alternatives: chilling of intact embryos and cryopreservation of isolated blastomeres. Cryopreservation was found to affect the normal pattern of gene expression in zebrafish embryonic blastomeres. The trends, profile changes, in ex... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547434 Sat, 02 May 2009 23:00:00 +0100 2547434 http://www.medworm.com/index.php?rid=2547438&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19394323%26dopt%3DAbstract Authors: Kaneko T, Fukumoto K, Haruguchi Y, Kondo T, Machida H, Koga M, Nakagawa Y, Tsuchiyama S, Saiki K, Noshiba S, Nakagata N The C57BL/6 mouse is commonly used to produce transgenic and knockout strains for biomedical research. However, the motility and fertility of its sperm decrease markedly with freezing. Short-term preservation of sperm without freezing can avoid this. Furthermore, such samples can be transported safety without the special skills or equipment needed for the transportation of live animals or frozen products. We evaluated the motility and fertility of sperm collected from cauda epididymides after preservation or transportation at 4 degrees C. Oocytes with the zona pellucida subjected to laser-microdissection were used to assist fertilization in vitro. Although th... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547438 Thu, 23 Apr 2009 23:00:00 +0100 2547438 http://www.medworm.com/index.php?rid=2547436&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19394324%26dopt%3DAbstract Authors: Gerelchimeg B, Li-Qing L, Zhong Z, Jiang-Tian T, Qing-Ran K, Jun S, Xue-Dong W, Zhong-Hua L The potential subcellular consequence of chilling on porcine germinal vesicle (GV) stage oocytes was examined. Prior to in vitro maturation (IVM), Cumulus-oocyte complexes (COCs) freshly collected from antral follicles (3-6mm in diameter) were evenly divided into four groups and immediately incubated in PVA-TL-HEPES medium at the temperature of 39 degrees C (control group), 23 degrees C (room temperature), 15 degrees C and 10 degrees C for 10min, respectively. Following 42h of IVM at 39 degrees C, the survival rates were examined. There was no significant difference between the survival rate of 23 degrees C chilled group and control group (77.92 and 91.89%), but the survival rate of 15 ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547436 Thu, 23 Apr 2009 23:00:00 +0100 2547436 http://www.medworm.com/index.php?rid=2547444&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19375414%26dopt%3DAbstract The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T(OIF)), extra-cellular ice formation (T(EIF)) and intracellular ice formation (T(IIF)) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T(EIF) and T(IIF) at high cooling rate were... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547444 Wed, 15 Apr 2009 23:00:00 +0100 2547444 http://www.medworm.com/index.php?rid=2547442&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19375415%26dopt%3DAbstract Authors: Elmoazzen HY, Lee GY, Li MW, McGinnis LK, Kent Lloyd KC, Toner M, Biggers JD It has been shown in the past that mouse spermatozoa could be dried under a stream of nitrogen gas at ambient temperature and stored at 4 degrees C or 22 degrees C for up to 3 months and was capable of generating live-born offspring. In previous desiccation work, dried sperm were stored in a vacuum-sealed plastic bag placed in a vacuum-packed Mylar bag. However, dried specimens stored in this way often lost moisture, particularly in samples stored at higher temperatures (22 degrees C) compared to lower temperatures (4 degrees C). The present report describes a method which minimizes this water loss from the dried sperm samples. Its use is described in a preliminary study on the effect of supplementing... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547442 Wed, 15 Apr 2009 23:00:00 +0100 2547442 http://www.medworm.com/index.php?rid=2547440&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19375416%26dopt%3DAbstract Authors: Barfield JP, McCue PM, Squires EL, Seidel GE Cryopreservation of equine embryos>300mum in diameter results in low survival rates using protocols that work well for smaller equine embryos. These experiments tested the potential benefit of incorporating a dehydration step prior to standard cryopreservation procedures. Forty-six, day 7-8, grade 1, equine embryos 300-1350mum in diameter were subjected to one of the following treatments: (A) 2 min in 0.6M galactose, 10min in 1.5M glycerol, slow freeze (n=21); (B) 10min in 1.5M glycerol, slow freeze (n=15); (C) 2min in 0.6M galactose, 10min in 1.5M glycerol, followed by exposure to thaw solutions, then culture medium (n=5); (D) transferred directly to culture medium (n=5). Frozen embryos were thawed and subjected to a three-step ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547440 Wed, 15 Apr 2009 23:00:00 +0100 2547440 http://www.medworm.com/index.php?rid=2547448&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19362084%26dopt%3DAbstract Authors: Thom F The most common method for measuring the mechanical behavior of the human red blood cell (RBC) membrane is micropipette aspiration, because it can be used to apply both a low uniaxial stress at a small part of the membrane or high two-axial stresses to the whole membrane [E.A. Evans, R.E. Waugh, Mechano-chemical study of red cell membrane structure in situ, in: Kroc Foundation Series, vol. 13, Erythrocyte Mechanics and Blood Flow, Alan R. Liss. Inc., New York, 1980, pp. 31-56 (Chapter 3); H.J. Meiselman, Measures of blood rheology and erythrocyte mechanics, in: Kroc Foundation Series, vol. 13, Erythrocyte Mechanics and Blood Flow, Alan R. Liss. Inc., New York, 1980, pp. 75-117 (Chapter 5)]. The elastic shear moduli and area changes of the human RBC published to date wer... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547448 Wed, 08 Apr 2009 23:00:00 +0100 2547448 http://www.medworm.com/index.php?rid=2547446&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19362085%26dopt%3DAbstract Authors: Hayashi M, Tsuchiya H, Otoi T, Agung B, Yamamoto N, Tomita K Improving survival rates for sarcoma patients are necessitating more functional and durable methods of reconstruction after tumor resection. Frozen osteoarticular grafts are utilized for joint reconstruction, but the joint may develop osteoarthritic change. We used a frozen autologous whole-rabbit knee joint graft model to investigate the influence of freezing on joint components. Thirty rabbit knee joints that had been directly immersed into liquid nitrogen (L) or saline (C) without use of cryoprotectants were re-implanted. Histological observations were made after 4, 8, and 12weeks. Both groups had bone healing. In group L, despite restoration of cellularity to the menisci and ligaments, no live chondrocytes were o... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2547446 Wed, 08 Apr 2009 23:00:00 +0100 2547446 http://www.medworm.com/index.php?rid=2324395&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19345683%26dopt%3DAbstract CONCLUSION: Though MP has been well introduced in organ transplantation, in our opinion, it must still be optimized and standardized. It is necessary to clarify questions such as whether there is a need for oxygenation during perfusion, the length of perfusion, the impact of pressure, and the effects of additional scavengers. The results of the present study suggest the reconsideration of the ROPtechnique for the preservation of predamaged donor grafts especially of NHBD and further studies, comparing MP and ROP upon long term preservation are strongly encouraged. PMID: 19345683 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2324395 Thu, 02 Apr 2009 04:00:00 +0100 2324395 http://www.medworm.com/index.php?rid=2295786&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19318090%26dopt%3DAbstract We report a study on the protective effects of intracellular and extracellular sugars during bovine sperm desiccation and the supplemental effects of the addition of an antioxidant (catalase) or a chelator (desferal). The goal of the study was to preserve mammalian sperm in a partially or completely desiccated state. Sperm loaded intracellularly with two different types of sugars, trehalose or sucrose, were dried with and without catalase and desferal and evaluated for motility and membrane integrity immediately after rehydration. Intracellular sugars were loaded using ATP induced poration. Drying was performed in desiccator boxes maintained at 11% relative humidity (RH). Results indicated that sperm exhibited improved desiccation tolerance if they were loaded with either intracellular tre... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2295786 Sat, 21 Mar 2009 04:00:00 +0100 2295786 http://www.medworm.com/index.php?rid=2295781&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19318091%26dopt%3DAbstract CONCLUSION: Viability of long preserved liver grafts can be augmented by transient hypothermic reconditioning using either machine perfusion or gaseous oxygen persufflation, both preventing initial mitochondrial dysfunction and subsequent tissue injury. PMID: 19318091 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2295781 Sat, 21 Mar 2009 04:00:00 +0100 2295781 http://www.medworm.com/index.php?rid=2295791&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19303403%26dopt%3DAbstract Authors: Roach KL, King KR, Uygun K, Hand SC, Kohane IS, Yarmush ML, Toner M Microwell array cytometry is a novel high-throughput experimental technique that makes it possible to correlate pre-stress cell phenotypes and post-stress outcomes with single cell resolution. Because the cells are seeded in a high density grid of cell-sized microwells, thousands of individual cells can be tracked and imaged through manipulations as extreme as freezing or drying. Unlike flow cytometry, measurements can be made at multiple time points for the same set of cells. Unlike conventional image cytometry, image analysis is greatly simplified by arranging the cells in a spatially defined pattern and physically separating them from one another. To demonstrate the utility of microwell array cytometry in t... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2295791 Wed, 18 Mar 2009 04:00:00 +0100 2295791 http://www.medworm.com/index.php?rid=2273129&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19285056%26dopt%3DAbstract Authors: Paynter SJ, Andrews KJ, Vinh NN, Kelly CM, Rosser AE, Amso NN, Dunnett SB Neural cells isolated from the brain have a number of research and clinical applications, including transplantation to patients with neurodegenerative conditions. Tissue supply is one of the major limiting factors to clinical transplantation. Cryopreservation of primary neural cells would improve supply, aid in organisation of transplantation surgery and facilitate research. To date, cryopreservation using standard methods has resulted in reduced yield and/or viability of primary neural tissue. In order to optimise freezing protocols specifically for such cells, the non-osmotic volume (V(b)), water permeability (L(p)) and permeability to cryoprotectant (P(cpa)) were determined. Murine foetal brain tissue... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2273129 Tue, 10 Mar 2009 04:00:00 +0100 2273129 http://www.medworm.com/index.php?rid=2258000&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19268658%26dopt%3DAbstract This study is the first attempt at sperm cryopreservation, as well as a further examination of frozen sperm fertility by the hamster test, applied to the maintenance of an Indian gerbil (Tatera indica) colony, which is a newly developing experimental animal. The osmotic tolerance of the spermatozoa was initially investigated by subjection to hypertonicity, up to 620 mOsm/kg, for 5 min at room temperature prior to freezing. Although the percentage of total motile sperm was not affected, that of progressive motile spermatozoa began to drop at 400 mOsm/kg, and a significant decrease was observed at 620 mOsm/kg (p < 0.01). According to these results, the osmolality of the solutions for the freezing experiment, in which 6 to 22% raffinose was present, was fixed at approximately 400 mOsm/kg. ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2258000 Wed, 04 Mar 2009 05:00:00 +0100 2258000 http://www.medworm.com/index.php?rid=2233263&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19248776%26dopt%3DAbstract Authors: Han X, Ma L, Benson J, Brown A, Critser JK PMID: 19248776 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2233263 Tue, 24 Feb 2009 05:00:00 +0100 2233263 http://www.medworm.com/index.php?rid=2216764&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19236858%26dopt%3DAbstract This study demonstrated that the culture conditions soon after the injection of sperm markedly influenced the subsequent development of embryos. Also, rat sperm after freeze-drying in TE buffer were preserved at 4 degrees C for long term without their deterioration. PMID: 19236858 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2216764 Fri, 20 Feb 2009 05:00:00 +0100 2216764 http://www.medworm.com/index.php?rid=2137753&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19168046%26dopt%3DAbstract Authors: Kanias T, Acker JP PMID: 19168046 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2137753 Sat, 03 Jan 2009 05:00:00 +0100 2137753 http://www.medworm.com/index.php?rid=2137754&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19168045%26dopt%3DAbstract Authors: Takeo T, Kaneko T, Haruguchi Y, Fukumoto K, Machida H, Koga M, Nakagawa Y, Takeshita Y, Matsuguma T, Tsuchiyama S, Shimizu N, Hasegawa T, Goto M, Miyachi H, Anzai M, Nakatsukasa E, Nomaru K, Nakagata N Cryopreservation of 2-cell embryos is an effective technology for storage of genetically engineered mouse strains. Transport of genetically engineered mice between laboratories has frequently been performed using such cryopreserved 2-cell embryos. However, the receiving laboratory requires proficient skills and special instruments to obtain live young from cryopreserved and transported embryos. Therefore, in this study, we tried to address the storage and transport of vitrified/warmed 2-cell embryos at a cold temperature. In cold storage experiments, the development rates of 2-c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2137754 Wed, 31 Dec 2008 05:00:00 +0100 2137754 http://www.medworm.com/index.php?rid=2131399&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19162000%26dopt%3DAbstract In this study, we evaluate the effect of vitreous cryopreservation of rabbit trachea, by comparing vitrification procedure with conventional computer-programmed slow freezing approaches. Harvested rabbit trachea were tailored and divided into groups and cryopreserved by vitrification and programmed freezing, respectively. The morphology and ultrastructure of the thawed tracheal fragments including HE dyes, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were studied to assess the integrity of the tracheal fragments. Morphological studies demonstrated that both cryopreservation procedure retained the integrity of trachea, both epithelial cells, cilia and cartilag... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2131399 Tue, 30 Dec 2008 05:00:00 +0100 2131399 http://www.medworm.com/index.php?rid=2131400&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19161999%26dopt%3DAbstract Authors: Kleinhans FW, Mazur P Typically, subzero permeability measurements are experimentally difficult and infrequently reported. Here we report an approach we have applied to mouse oocytes. Interrupted cooling involves rapidly cooling oocytes (50 degrees C/min) to an intermediate temperature above the intracellular nucleation zone, holding them for up to 40min while they dehydrate, and then rapidly cooling them to -70 degrees C or below. If the intermediate holding temperature and holding time are well chosen, high post thaw survival of the oocytes is possible because the freezable water is removed during the hold. The length of time required for the exit of the freezable water allows the water permeability at that temperature to be determined. These experiments used 1.5M ethylene g... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2131400 Thu, 25 Dec 2008 05:00:00 +0100 2131400 http://www.medworm.com/index.php?rid=2129085&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19159623%26dopt%3DAbstract Authors: Amorim EA, Graham JK, Amorim LS, Spizirri B, Meyers M, Torres CA Different cholesterol conjugates-loaded-cyclodextrin was added to bull sperm to improve sperm quality after freezing. Ejaculates from four bulls were diluted to 120 million cells/ml in Tris (T) diluent and then sub-divided into 10 treatments as follow: no additive (control); 1.5mg CLC (positive control); 0.75mg or 1.5mg of cyclodextrin pre-loaded with cholesterol conjugated to heptanoate, palmitate, pelargonate or stearate, and incubated for 15min at 22 degrees C. The samples were then diluted 1:1 with 20% egg yolk (EY) in T diluent and cooled to 5 degrees C over a 2h. Upon reaching 5 degrees C, the samples were diluted 1:1 with T diluent containing 10% EY+16% glycerol, and allowed to equilibrate for 15min, and p... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2129085 Thu, 25 Dec 2008 05:00:00 +0100 2129085 http://www.medworm.com/index.php?rid=2121896&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152796%26dopt%3DAbstract In this study, over 1.0x10(8)cells/ml PBMC were cryopreserved as long as 52 weeks using a controlled-rate freezer (CRF) and stored in a vapor phase of liquid nitrogen tank. The effect of PBMC cryopreservation on differentiation into DC was studied by comparing the phenotypic and functional properties of immature DC (iDC) and mature DC (mDC) derived from cryopreserved PBMC to those from fresh PBMC. The results show that cryopreservation of PBMC at a fairly high cell concentration does not significantly affect cell recovery, viability, or phenotypes of PBMC. After differentiation into DC, iDC and mDC derived from cryopreserved PBMC had their typical phenotypes and function equivalent to those derived from fresh PBMC. Therefore, the improved cryopreservation process of PBMC described in this ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2121896 Thu, 25 Dec 2008 05:00:00 +0100 2121896 http://www.medworm.com/index.php?rid=2107814&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19141297%26dopt%3DAbstract In conclusion, alpha-tocopherol, supplemented at 200muM concentration in boar semen extender during cryopreservation had a positive effect on post-thawed sperm survivability. PMID: 19141297 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2107814 Thu, 25 Dec 2008 05:00:00 +0100 2107814 http://www.medworm.com/index.php?rid=2104647&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19138683%26dopt%3DAbstract Authors: Redden E, Davey R, Borjigin U, Hutton K, Hinch G, Hope S, Hill J, Herrid M Cryopreservation has become an integral component of any cell transplantation technique helping to overcome the issues associated with known spatial and temporal barriers between donor and recipient. The aim of this study was to develop a protocol for large quantity cryopreservation of bovine testicular germ cells. The impact of 3 different packaging methods (5ml semen straw, 20ml freezing bag and 1.5ml cryovial) and varying cell densities (3x10(6), 9x10(6), or 18x10(6)cells/ml) on the survival of testis germ cells was examined. Cells processed in 5 ml semen straws had a significantly higher viability (70.7+/-1.2%, P<0.05) compared to those cells in 20ml freezing bags (46.7+/-0.1%) or 1.5ml cryovials... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2104647 Thu, 25 Dec 2008 05:00:00 +0100 2104647 http://www.medworm.com/index.php?rid=2101165&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135990%26dopt%3DAbstract Authors: Kanias T, Acker JP One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study was to further investigate the extent of oxidative injury during the loading of trehalose into RBCs. RBCs were incubated in the absence (control) or presence of trehalose (0.8mol/l) at 4 degrees C or 37 degrees C for different time scales. Oxidative damage was monitored by flow cytometry using dichlorofluorescin for reactive oxygen species formation, Annexin V-FITC for phosphatidylserine translocation and fluorescein-DHPE for lipid peroxidation. ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2101165 Wed, 24 Dec 2008 05:00:00 +0100 2101165 http://www.medworm.com/index.php?rid=2101164&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135991%26dopt%3DAbstract In this study, AFPs were delivered in early zebrafish embryos by incubation in media containing protein. Their cryoprotective effects were then analyzed. Chilling sensitivity was evaluated at 4 degrees C and -10 degrees C. Survival rates significantly increased in embryos incorporating AFPI and kept at -10 degrees C. To analyze their effects on cryopreservation, 5-somite embryos were vitrified. Incorporation of AFPI reduced the percentage of embryos that collapsed at thawing (14.2% of AFPI-treated embryos and 48.9% of controls). Cellular damage caused by vitrification was assessed after thawing by cell dissociation and further analysis of cell survival in culture (SYBR-14/IP labeling). The percentage of viable cells at thawing ranged from 25 to 50%, considered incompatible with embryo deve... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2101164 Wed, 24 Dec 2008 05:00:00 +0100 2101164 http://www.medworm.com/index.php?rid=2090087&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19126409%26dopt%3DAbstract We examined factors potentially affecting their survival, including the growth phase, ice-seeding temperature, intracellular trehalose content, freezing period, and duration of supercooling. The results showed that the ice-seeding temperature significantly affected cell viability. In the stationary phase, trehalose accumulation did not affect the viability of yeast cells after brief freezing, although it did significantly affect the viability after prolonged freezing. In the log phase, the ice-seeding temperature was more important for cell survival than the presence of trehalose during prolonged freezing. The importance of increasing the extracellular ice-seeding temperature was verified by comparing frozen yeast survival rates in a freezing test with ice-seeding temperatures of -5 degree... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2090087 Wed, 24 Dec 2008 05:00:00 +0100 2090087 http://www.medworm.com/index.php?rid=2084142&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19121299%26dopt%3DAbstract Authors: Raymond JA, Janech MG Fungi have developed a variety of mechanisms for tolerating cold, including production of proteins that bind to ice, as shown by their ability to slightly lower the freezing point. At present, only one of these proteins, from the snow mold, Typhula ishikariensis, and partial transcripts of a similar protein from shiitake mushroom, Lentinula edodes, have been identified. Here, we report the full sequences of ice-binding proteins from shiitake and another mushroom, the cold-adapted Flammulina populicola (enoki mushroom), and show that the recombinant proteins have ice-binding activity. The three proteins share 50-55% identities and are similar to other ice-binding proteins recently identified in ice bacteria and sea ice diatoms. The possibility that ice-bin... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2084142 Tue, 16 Dec 2008 05:00:00 +0100 2084142 http://www.medworm.com/index.php?rid=2078268&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19118541%26dopt%3DAbstract Authors: Seki S, Kleinhans FW, Mazur P To survive freezing, cells must not undergo internal ice formation during cooling. One vital factor is the cooling rate. The faster cells are cooled, the more their contents supercool, and at some subzero temperature that supercooled cytoplasm will freeze. The question is at what temperature? The relation between cooling rate and cell supercooling can be computed. Two important parameters are the water permeability (Lp) and its temperature dependence. To avoid intracellular ice formation (IIF), the supercooling must be eliminated by dehydration before the cell cools to its ice nucleation temperature. With an observed nucleation temperature of -25 degrees C, the modeling predicts that IIF should not occur in yeast cooled at <20 degrees C/min and... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2078268 Thu, 11 Dec 2008 05:00:00 +0100 2078268 http://www.medworm.com/index.php?rid=2074513&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19111715%26dopt%3DAbstract Authors: Kurtmann L, Carlsen CU, Risbo J, Skibsted LH Storage stability of freeze-dried Lactobacillus acidophilus was found to depend on water activity (0.11-0.43), oxygen level (atmospheric oxygen level and <4% oxygen compared) and presence of sodium ascorbate (0% and 10% (w/w)). Increasing water activities decreased bacterial survival, and a reduced oxygen level (<4% oxygen) improved the storage stability, which strongly indicates a connection between oxidative reactions and bacterial instability. The detrimental effect of atmospheric oxygen was reduced by including ascorbate in the freeze drying medium. However, when ascorbate was present a pink/red colour was observed on the surface of the dried samples increasing with the water activity and oxygen level. Increased water acti... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2074513 Thu, 11 Dec 2008 05:00:00 +0100 2074513 http://www.medworm.com/index.php?rid=2074512&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19114036%26dopt%3DAbstract Authors: Muchlisin ZA Study on the effect of cryoprotectants on abnormality and motility of baung, Mystus nemurus spermatozoa were evaluated using transmission and scanning electron microscopy. Four cryoprotectants, dmso, ethanol, methanol and glycerol at concentration of 10% were tested in triplicates. Three ml of fresh sperm which was diluted with 60ml of ringer solution was added to each of twelve 5-ml vials containing of 0.50-ml of the cryoprotectants. The vials were placed in an icebox containing dry ice 5min and then storage into container containing liquid nitrogen for 13 months. The effect of cryoprotectants on the spermatozoa abnormality and motility were significant (P<0.05). The spermatozoa abnormality was significantly lower in methanol (62.65%) compared with the other c... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2074512 Thu, 11 Dec 2008 05:00:00 +0100 2074512 http://www.medworm.com/index.php?rid=2074514&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19111714%26dopt%3DAbstract This study is a part of an ongoing effort to develop computerized tools for cryosurgery. Based on typical clinical practices, previous automated planning schemes have required that all cryoprobes be aligned at a single insertion depth. The current study investigates the benefit of removing this constraint, in comparison with results based on uniform insertion depth planning as well as the so-called "pullback procedure". Planning is based on the so-called "bubble-packing method", and its quality is evaluated with bioheat transfer simulations. This study is based on five 3D prostate models, reconstructed from ultrasound imaging, and cryoprobe active length in the range of 15-35mm. The variable insertion depth technique is found to consistently provide superior results when compared to the ot... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2074514 Wed, 10 Dec 2008 05:00:00 +0100 2074514 http://www.medworm.com/index.php?rid=2067760&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19109940%26dopt%3DAbstract Authors: Buchinger S, Campen E, Helmers E, Morosow V, Krefft M, Reifferscheid G The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab independent device for the detection of genotoxic compounds in field studies with an integrated module for the metabolic activation of pre-genotoxic compounds. The carbohydrates sucrose, trehalose and raffinose were tested in different concentrations or mixtures in order to increase the product stability of the S9-fraction during and after freeze-drying. The activity of the freeze-dried S9-sam... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2067760 Wed, 10 Dec 2008 05:00:00 +0100 2067760 http://www.medworm.com/index.php?rid=2061168&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19101533%26dopt%3DAbstract Authors: Di Matteo O, Langellotti AL, Masullo P, Sansone G The effects of cryoprotectants, cooling rate and freezing on the mussel Mytilus galloprovincialis sperm were evaluated. At the end of each step of the experimental protocol, motility and fertilization ability of sperm were analyzed, compared to fresh semen. Five cryoprotectants were tested in their toxicity level: dimethylsulfoxide, ethylene glycol, 1-2 propylene glycol at 5%, 7%, 10%, 15% and 20% concentration; glycerol and methanol at concentration of 5%, 7% and 10%. The incubation times were 10, 20 and 30min at 20+/-1 degrees C. Only dimethylsulfoxide, ethylene glycol and 1-2 propylene glycol at 5%, 7% and 10% were chosen for the following pre-freezing step. Five adaptation/chilling rates were analyzed: 10min at 20+/-1, -2, ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2061168 Sun, 07 Dec 2008 05:00:00 +0100 2061168 http://www.medworm.com/index.php?rid=2056183&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19094979%26dopt%3DAbstract Authors: Ehrhart F, Schulz JC, Katsen-Globa A, Shirley SG, Reuter D, Bach F, Zimmermann U, Zimmermann H Cryopreservation of human tumour cells and tissue is a valuable tool for retrospective analysis and for the transport and handling of biopsy material. Tumour tissue consists of different cell types, which have different optimal freezing conditions, and extracellular matrix. A well-defined and authentic model system is required for developing new freezing protocols and media. This work describes the use of L929 and PC-3 spheroids as new model systems for freezing human tumours. Cell suspension and spheroids were frozen in different vessels (1ml cryovials and a special, cryo-compatible 30x25mul multi well plate) at slow rate (1 degrees C/min). Freezing media were combinations of cultur... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2056183 Wed, 03 Dec 2008 05:00:00 +0100 2056183 http://www.medworm.com/index.php?rid=2047124&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19070613%26dopt%3DAbstract Authors: Sarıözkan S, Bucak MN, Tuncer PB, Ulutaş PA, Bilgen A Oxidative stress significantly damages sperm functions such as motility, functional integrity, endogenous antioxidant enzyme activities and fertility due to lipid peroxidation induced by reactive oxygen species (ROS). The aim of this study was to determine the effects of antioxidants such as taurine and cysteine in Bioxcell((R)) extender on standard semen parameters, fertilizing ability, lipid peroxidation (LPO) and antioxidant activities comprising reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) after the cryopreservation/thawing of bull semen. Nine ejaculates for each bull were included in the study. Three groups, namely taurine (2mM), cysteine (2m... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2047124 Wed, 03 Dec 2008 05:00:00 +0100 2047124 http://www.medworm.com/index.php?rid=2006053&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19041639%26dopt%3DAbstract CONCLUSION: The results of this experiment provide accurate permeation kinetics of four commonly used CPAs in porcine articular cartilage. This information will be useful for developing effective vitrification protocols for cryopreservation of AC. PMID: 19041639 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=2006053 Tue, 18 Nov 2008 05:00:00 +0100 2006053 http://www.medworm.com/index.php?rid=2006055&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19041300%26dopt%3DAbstract Authors: Stott SL, Karlsson JO A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attachment to reproducible circular or rectangular domains. Employing frame rates of 8000frames/s and 16,000frames/s to record IIF in micropatterned and suspended cells, respectively, intracellular crystal growth manifested as a single advancing front that initiated from a point source within the cell, and traveled at velocities of 0.0006-0.023m/s. Whereas this primary crystallization process resulted in minimal change in cell opacity, the well-kn... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2006055 Mon, 17 Nov 2008 05:00:00 +0100 2006055 http://www.medworm.com/index.php?rid=2006054&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19041638%26dopt%3DAbstract Authors: Piasecki BP, Diller KR, Brand JJ Cryopreservation is a practical method for stabilizing the genetic content of living algae over long periods of time. Yet, Chlamydomonas reinhardtii, the algal species most often utilized in studies requiring genetically defined strains, is difficult to cryopreserve with a consistently high post-thaw viability. Work described here demonstrates that C. reinhardtii retains high viability only when cryopreserved at a low cell density. Low viability at high cell density was caused by the release of an injurious substance into the culture medium. Rapid freezing and thawing under non-cryoprotective conditions released large amounts of the injurious substance. Heat denaturation of cells prevented the release of the injurious substance, but heating did... Cryobiology journals http://www.medworm.com/rss/comments.php?id=2006054 Thu, 13 Nov 2008 05:00:00 +0100 2006054 http://www.medworm.com/index.php?rid=1991043&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19026625%26dopt%3DAbstract Authors: Wang HY, Lu SS, Lun ZR Knowledge of the glass transition behavior of vitrification solutions is important for research and planning of the cryopreservation of biological materials by vitrification. This brief communication shows the analysis for the glass transition and glass stability of the multi-component vitrification solutions containing propanediol (PE), dimethyl sulfoxide (Me(2)SO) and polyvinyl alcohol (PVA) by using differential scanning calorimetry (DSC) during the cooling and subsequent warming between 25 and -150 degrees C. The glass formation of the solutions was enhanced by introduction of PVA. Partial glass formed during cooling and the fractions of free water in the partial glass matrix increased with the increasing of PVA concentration, which caused slight dec... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1991043 Thu, 06 Nov 2008 05:00:00 +0100 1991043 http://www.medworm.com/index.php?rid=1991042&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19026626%26dopt%3DAbstract Authors: Adams SL, Tervit HR, McGowan LT, Smith JF, Roberts RD, Salinas-Flores L, Gale SL, Webb SC, Mullen SF, Critser JK Cryopreservation is a powerful tool for selective breeding in aquaculture as it enables genetic material from selected stock to be stored and crossed at will. The aim of this study was to develop a method for cryopreserving oocytes of the Greenshelltrade mark mussel (Perna canaliculus), New Zealand's main aquaculture species. The ability of oocytes to be fertilized post-thawing was used as the criterion for success in initial experiments and then subsequently, the ability of frozen oocytes to develop further to D-stage larvae was assessed. Ethylene glycol, propylene glycol, dimethyl sulphoxide and glycerol were evaluated at a range of concentrations with and without... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1991042 Thu, 06 Nov 2008 05:00:00 +0100 1991042 http://www.medworm.com/index.php?rid=1981000&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19017529%26dopt%3DAbstract Authors: Muldrew K, Schachar J, Cheng P, Rempel C, Liang S, Wan R It has been hypothesized that pores in the plasma membrane form under conditions of rapid water efflux, allowing extracellular ice to grow into the cytoplasm under conditions of rapid freezing. When cells with intracellular ice are thawed slowly, the transmembrane ice crystal expands through recrystallization causing the cell to lyse. One of the implications of this hypothesis is that osmotic pores will provide an alternative route for water movement under conditions of osmotically induced flow. We show that the plasma membrane water permeability of a fibroblast cell changes as a function of the osmotic pressure gradient that is used to drive water movement. It is further shown that cell volume is more important than the... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1981000 Wed, 05 Nov 2008 05:00:00 +0100 1981000 http://www.medworm.com/index.php?rid=1952109&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18992734%26dopt%3DAbstract This study also represents the first success of freezing without permeable cryoprotectant in non-human primates. PMID: 18992734 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=1952109 Tue, 21 Oct 2008 04:00:00 +0100 1952109 http://www.medworm.com/index.php?rid=1939301&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18983838%26dopt%3DAbstract Authors: Heikal A, Box K, Rothnie A, Storm J, Callaghan R, Allen M The drug efflux pump P-glycoprotein (P-gp) (ABCB1) confers multidrug resistance, a major cause of failure in the chemotherapy of tumours, exacerbated by a shortage of potent and selective inhibitors. A high throughput assay using purified P-gp to screen and characterise potential inhibitors would greatly accelerate their development. However, long-term stability of purified reconstituted ABCB1 can only be reliably achieved with storage at -80 degrees C. For example, at 20 degrees C, the activity of ABCB1 was abrogated with a half-life of <1 day. The aim of this investigation was to stabilise purified, reconstituted ABCB1 to enable storage at higher temperatures and thereby enable design of a high throughput assay sys... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1939301 Fri, 17 Oct 2008 04:00:00 +0100 1939301 http://www.medworm.com/index.php?rid=1934130&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18976647%26dopt%3DAbstract The objective of this work was to select and test systematically possible cryoprotective agents (CPAs) and to obtain a suitable formula for vitrification of corneal endothelial cells (CECs). Fresh bovine CECs were isolated and tested with an optimized vitrification protocol with multi-step CPA loading and removal. Three types of CPAs components, i.e. the penetrating CPAs, sugars and macromolecular compounds, were experimentally evaluated using the viability assayed by trypan blue. Dimethyl sulfoxide, ethylene glycol (EG), 1,2-propanediol, 2,3-butanediol, acetamide and ethylene glycol monomethyl ether were chosen as the penetrating CPA components. Sugars including xylose, fructose, mannose, glucose, maltose, sucrose and trehalose were tested. Ficoll (MW 7kDa), dextran (MW 7kDa), chondroitin... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1934130 Fri, 17 Oct 2008 04:00:00 +0100 1934130 http://www.medworm.com/index.php?rid=1934129&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18977212%26dopt%3DAbstract Authors: Yoshimori T, Takamatsu H Cell dehydration during freezing results from an elevated concentration of electrolytes in the extracellular medium that is deeply involved in cellular injury. We undertook real-time threedimensional (3-D) observation of osmotic dehydration of cells, motivated by a comparison of cellular responses between isolated cells in suspension and cultured cells adhering to a surface since several studies have suggested a difference in freeze tolerance between cell suspensions and monolayers. A laser confocal scanner was used with a perfusion microscope to capture sectional images of chloromethylbenzamido (DiI)-stained PC-3 cells that were exposed to an increase in NaCl concentration from 0.15 to 0.5M at 23 degrees C. Change in cell volume was determined from re... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1934129 Fri, 17 Oct 2008 04:00:00 +0100 1934129 http://www.medworm.com/index.php?rid=1934128&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18977213%26dopt%3DAbstract In conclusion, the results of the present study recommend the use of gaseous oxygen persufflation to improve tissue integrity and functional recovery of predamaged livers. PMID: 18977213 [PubMed - as supplied by publisher] (Source: Cryobiology) Cryobiology journals http://www.medworm.com/rss/comments.php?id=1934128 Fri, 17 Oct 2008 04:00:00 +0100 1934128 http://www.medworm.com/index.php?rid=1914820&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18950613%26dopt%3DAbstract Authors: Yang G, Zhang A, Xu LX Study of the intracellular ice formation (IIF) and growth is essential to the mechanistic understanding of cellular damage through freezing. In the aid of high speed and high resolution cryo-imaging technology, the transient intracellular ice formation and growth processes of the attached human umbilical vein endothelial cells (HUVEC) were successfully captured during freezing. It was found that the intracellular ice nucleation site was on the cell membrane closer to the nucleus. The ice growth was directional and toward the nucleus, which covered the whole nucleus before growing into the cytoplasm. The crystal growth rate in the nucleus was much larger than that in the cytoplasm, and its morphology was influenced by the cooling rate. During the thawing ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1914820 Fri, 10 Oct 2008 04:00:00 +0100 1914820 http://www.medworm.com/index.php?rid=1914819&cid=s_35487_62_f&fid=35487&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18951888%26dopt%3DAbstract Authors: Hagedorn M, Ricker J, McCarthy M, Meyers SA, Tiersch TR, Varga ZM, Kleinhans FW In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically ... Cryobiology journals http://www.medworm.com/rss/comments.php?id=1914819 Fri, 10 Oct 2008 04:00:00 +0100 1914819