MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Diagnostic Cytopathology' source. Thu, 09 Feb 2012 00:42:10 +0100 http://www.medworm.com/index.php?rid=5651037&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22832 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651037 Wed, 01 Feb 2012 05:00:00 +0100 5651037 http://www.medworm.com/index.php?rid=5651036&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22826 We report a case of mediastinal seminoma diagnosed intraoperatively by cytological examination of cystic fluid containing tumor cells.An anterior mediastinal tumor with cystic component was incidentally found in a 28‐year‐old Japanese male. Cytological examination of the cystic fluid at the time of tumor resection showed single and loose aggregates of large round to polygonal cells with large round nuclei, vesicular chromatin and nucleoli, and mild to moderate amounts of PAS‐positive cytoplasm admixed with mature lymphocytes. A diagnosis of seminoma was made intraoperatively. On subsequent immunostaining, the tumor cells showed nuclear positivity for SALL4, a recently recognized germ cell marker that, in the appropriate setting, can be helpful to distinguish germ cell tumors from oth... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651036 Wed, 01 Feb 2012 05:00:00 +0100 5651036 http://www.medworm.com/index.php?rid=5651035&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22825 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651035 Wed, 01 Feb 2012 05:00:00 +0100 5651035 http://www.medworm.com/index.php?rid=5651034&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22824 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651034 Wed, 01 Feb 2012 05:00:00 +0100 5651034 http://www.medworm.com/index.php?rid=5651033&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22823 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651033 Wed, 01 Feb 2012 05:00:00 +0100 5651033 http://www.medworm.com/index.php?rid=5651032&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22816 We report the cytopathologic findings of a case of metastatic signet‐ring cell carcinoma with abundant extracellular mucin production in the mediastinal lymph nodes of a 41‐year‐old woman, who presented with nausea, abdominal pain, and weight loss. Imaging studies showed a renal mass, numerous lung nodules, and mediastinal and retroperitoneal lymphadenopathy. EBUS‐TBNA of level 4R and 7 lymph nodes showed abundant, thick, “clean” mucus with entrapped ciliated bronchial cells, rare histiocytes, and fragments of cartilage. No neoplastic cells could be identified in Diff‐Quik®‐stained smears during the rapid on‐site evaluation, but rare signet‐ring cells were identified in the Papanicolaou‐stained smears and cellblock sections. A distinctive feature of the aspirates was... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5651032 Wed, 01 Feb 2012 05:00:00 +0100 5651032 http://www.medworm.com/index.php?rid=5639886&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21775 This report highlights that the presence of abundant cell debris, degenerative cells and apoptotic bodies, and the absence of vivid mesothelial cells are the key cytological findings to suspect HSV peritonitis, and the diagnosis can be confirmed by careful surveillance for characteristic nuclear findings of single or multinucleated cells. The frequency of opportunistic infection is increased because of the increased numbers of iatrogenic immunocompromised patients as seen in this case, therefore, cytological examination is a useful method for early detection of the causative agent of peritonitis including HSV. Diagn. Cytopathol. 2011; © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5639886 Mon, 30 Jan 2012 06:31:20 +0100 5639886 http://www.medworm.com/index.php?rid=5585680&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22818 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585680 Thu, 12 Jan 2012 05:00:00 +0100 5585680 http://www.medworm.com/index.php?rid=5585679&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22815 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585679 Thu, 12 Jan 2012 05:00:00 +0100 5585679 http://www.medworm.com/index.php?rid=5585678&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22814 We report two cases of PSCCE and review the literature regarding associated markers and treatment outcomes. Many different markers have been tested for association with PSCCE, with mixed results. Thus, it is likely that several etiologic factors are responsible for the development of PSCCE. Further, due to the rarity of the condition, the optimal postoperative management of patients with PSCCE remains to be defined. Diagn. Cytopathol. 2012; © 2012 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585678 Thu, 12 Jan 2012 05:00:00 +0100 5585678 http://www.medworm.com/index.php?rid=5585677&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22813 AbstractIntrapancreatic accessory spleen is not an uncommon entity and usually located in the tail of the pancreas. Most of them are asymptomatic and incidental findings on radiologic study or at autopsy. On imaging study, it appears to be a well‐defined, solitary, and hypervascular lesion; therefore, it may be confused with pancreatic neoplasms, such as neuroendocrine neoplasm, well‐differentiated adenocarcinoma, solid pseudopapillary tumor, or metastatic tumor to the pancreas. As such, the diagnostic fine‐needle aspiration biopsy of the lesion may be performed. Several case reports describing cytological features of the lesion have been published in recent years. Among them, the most commonly identified cytological findings are sheets of a heterogeneous population of lymphocytes an... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585677 Thu, 12 Jan 2012 05:00:00 +0100 5585677 http://www.medworm.com/index.php?rid=5585676&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21739 AbstractThe patient is a 75‐year‐old man with axillary lymphadenopathy presenting an indurated papule on his buttock. Touch imprint cytology of the biopsied axillary lymph node revealed the monotonous appearance of medium‐sized tumor cells. The nuclei had a slightly irregular contour, finely dispersed chromatin, and a conspicuous nucleolus. Some tumor cells had intracytoplasmic microvacuoles. Immunohistochemistry of the imprint specimens showed that the tumor cells were positive for CD56 and CD123. Histological diagnosis of the lesion was blastic plasmacytoid dendritic cell neoplasm (BPDCN). Epstein‐Barr virus‐encoded RNAs were not detected in the tumor cells. Neither immunoglobulin heavy chain genes nor T‐ cell receptor genes was clonally rearranged. BPDCN should be strongly c... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585676 Thu, 12 Jan 2012 05:00:00 +0100 5585676 http://www.medworm.com/index.php?rid=5585674&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21738 This study was to clarify the nuclear features of “condensed clusters of stromal cells (EGBD‐stromal cells)” and “metaplastic clumps with irregular protrusions (EGBD‐metaplastic cells)” which may be recognized in endometrial glandular and stromal breakdown (EGBD) cases in liquid‐based cytologic (LBC) preparations of endometrial brushings. The material consists of cytologic smears of 20 cases of proliferative endometrium (PE), 20 cases of EGBD, and 20 cases of endometrioid adenocarcinoma grade 1 (G1) for which histopathological diagnosis was obtained by endometrial curettage. Nuclear findings were examined in PE cells, EGBD‐stromal cells, EGBD‐metaplastic cells, and G1 cells, respectively.It was examined about the following items: (1) Nuclear shape; (2) A long/minor axis r... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5585674 Sun, 01 Jan 2012 05:00:00 +0100 5585674 http://www.medworm.com/index.php?rid=5478702&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22806 We report the clinicopathological, cytologic, and histologic findings of a clear cell parathyroid tumor in a 64‐year‐old HIV‐positive patient. A computed tomography (CT) scan with contrast showed a heterogeneous and enhancing mass at the inferolateral aspect of left thyroid lobe. FNA showed a cellular smear with many single and loosely clustered tumor cells with finely granular and vacuolated light‐purple cytoplasm and central nuclei. Occasional microfollicular structures were noted. No colloid was seen. This FNA was misdiagnosed as a follicular neoplasm of the thyroid. Sections of the excised mass showed large polyhedral cells with well‐defined cell membranes and clear cytoplasm with a small amount of eosinophilic granular material. These clear cells were positive for pancytoker... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478702 Mon, 05 Dec 2011 05:00:00 +0100 5478702 http://www.medworm.com/index.php?rid=5478700&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22805 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478700 Mon, 05 Dec 2011 05:00:00 +0100 5478700 http://www.medworm.com/index.php?rid=5478697&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22804 This study was undertaken to assess the best pre‐analytical procedure and to define a reliable reference value for FNAC‐CT. Fifty‐four patients underwent thyroid surgery, so MTC was excluded by surgical pathology examination and CT immunostains. Before surgery, FNAC‐CT was measured on 78 thyroid nodules from such 54 patients. Needles were rinsed by normal saline and specific CT‐free dilution buffer, and CT was measured by a fully automated immunochemiluminometric assay. FNAC‐CT levels were not significantly different in normal saline or specific buffer. The calculated 97.5th upper FNAC‐CT value was 8.5 pg/mL (saline) and 7.43 pg/mL (buffer), respectively. Seeing its relatively large sample size, rigorous selection criteria and inclusion of CT immunostaining of thyroid nodules... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478697 Mon, 05 Dec 2011 05:00:00 +0100 5478697 http://www.medworm.com/index.php?rid=5478696&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22803 AbstractSquamous metaplasia (SM) occurs in a variety of thyroid conditions, both neoplastic and non‐neoplastic. In a small subset of benign thyroid lesions, SM can create a diagnostic pitfall by mimicking a malignant process. Here, we describe the case of a 52‐year‐old male with a 1.5 cm right thyroid nodule. Ultrasound‐guided fine‐needle aspiration biopsy showed markedly atypical, mitotically active epithelial cells which were suspicious for high‐grade carcinoma. Resection of the nodule demonstrated a follicular adenoma with cystic changes and atypical SM of the cyst‐lining component. As illustrated here, SM of the thyroid represents an important diagnostic pitfall for the cytopathologist, and should be included in the differential diagnosis of thyroid nodules, particularly ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478696 Mon, 05 Dec 2011 05:00:00 +0100 5478696 http://www.medworm.com/index.php?rid=5478693&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22808 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478693 Mon, 05 Dec 2011 05:00:00 +0100 5478693 http://www.medworm.com/index.php?rid=5478691&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22807 In conclusion, direct smears represent a feasible and robust source of cellular material for immunocytochemical studies to diagnose Merkel cell carcinoma. This methodology allows the cytologist to confirm on site that material for diagnostic immunocytochemistry is present thereby serving as a safeguard in instances where insufficient cell block cellularity is anticipated or encountered. Diagn. Cytopathol. 2011; © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478691 Mon, 05 Dec 2011 05:00:00 +0100 5478691 http://www.medworm.com/index.php?rid=5478689&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22812 This study has looked in to the diagnostic significance of PBs in fine needle aspiration (FNAC) of breast lesions. Over a 5‐year period, FNACs of the breast were done in 4,563 subjects, of which 1,678 were diagnosed to be malignant. On review of all breast aspirates including non‐neoplastic lesions, 30 cases showed PBs to be associated with breast carcinoma (BC). Cytological features were correlated with clinical, radiological, histological, and immunohistochemical findings. All 30 aspirates and their corresponding histological sections showed varying number of PBs and nonpsammomatous bodies (NPBs). For comparison, 31 cases of age‐matched BC without PBs and NPBs in both aspirates and sections were studied. Statistical analysis using Chi‐square test was done to compare BC with and w... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478689 Mon, 05 Dec 2011 05:00:00 +0100 5478689 http://www.medworm.com/index.php?rid=5478686&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.22811 AbstractPrimary strumal carcinoid tumor of the ovary (SCTO) is an extremely rare entity, though the survival rate is excellent if the disease is confined to one ovary. A case is presented here in which intraoperative squash smears in a 45‐year‐old woman with a left adnexal mass revealed dispersed or small clusters of neoplastic cells forming loosely cohesive gland‐like structures with abundant cytoplasm. The nuclear chromatin was finely granular with a “salt and pepper” appearance and occasional tiny nucleoli. The position of the nucleus presented a vaguely plasmacytoid appearance. Small fragments of thyroidal colloid‐like structures were also identified. A cytopathologic diagnosis of a SCTO was suggested. Further evaluation and immunohistochemical studies were conducted on for... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5478686 Thu, 01 Dec 2011 05:00:00 +0100 5478686 http://www.medworm.com/index.php?rid=5424277&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21849 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424277 Sat, 19 Nov 2011 17:47:47 +0100 5424277 http://www.medworm.com/index.php?rid=5424289&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21789 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424289 Fri, 18 Nov 2011 05:00:00 +0100 5424289 http://www.medworm.com/index.php?rid=5424288&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21863 AbstractReporting of benign appearing endometrial cells (BECs) in the Papanicolaou smears of women aging 40 years or older was mandated in the Bethesda System 2001 aiming at predicting the uterine pathology. The purpose of this study was to determine the clinical significance of the BECs in patients in our Medical Center. A two‐arm study was designed in ≥40‐years‐old women with BECs and without BECs in their Pap smears from January 2002 to December 2004. Of 21,965 patients, 882 had BECs in their Pap smears and the rest did not. Among the patients with BECs, 186 (study group) and among those without BECs, 2,064 (control group) had histopathologic follow‐ups. There were 4 patients in the study and 47 in the control groups who had uterine adenocarcinoma. The Chi‐square P‐value f... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424288 Fri, 18 Nov 2011 05:00:00 +0100 5424288 http://www.medworm.com/index.php?rid=5424287&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21862 AbstractExtramedullary hematopoiesis (EMH) is the production of mature blood elements outside of the bone marrow and can occur as a compensatory result of a marrow replacing process or from marrow space occupying lesions such as tumor or marrow fibrosis. EMH can also be induced by factors elicited by neoplasms, such as vascular endothelial growth factor (VEGF). Usually, EMH is a diffuse process most commonly observed in lymph nodes, liver, and spleen. Rarely, EMH can form a mass lesion. Although the spleen is a common site for diffuse EMH, it is a rare location for a mass forming EMH. Hemangiomas are the most common benign tumors of the spleen.A case of a discrete, 8 cm lesion was noted incidentally on CT scan in a 59‐year‐old man with no significant past medical history. Endoscopic ul... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424287 Fri, 18 Nov 2011 05:00:00 +0100 5424287 http://www.medworm.com/index.php?rid=5424286&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21858 We report a case of a woman who presented with a retroperitoneal lymph node involved by metastatic gangliocytic paraganglioma. Subsequently, fine‐needle aspiration (FNA) cytology was used to identify the primary duodenal gangliocytic paraganglioma. The smears of the aspirate material were highly cellular and contained a dominant population of epithelioid cells, a second population of ganglion cells and a third population of small, bland spindled cells. To our knowledge, the cytologic features of gangliocytic paraganglioma have not previously been documented. Diagn. Cytopathol. 2011. © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424286 Fri, 18 Nov 2011 05:00:00 +0100 5424286 http://www.medworm.com/index.php?rid=5424285&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21857 AbstractThere is a paucity of prospective data on flexible bronchoscopy with rapid on‐site evaluation (ROSE) in the setting of superior vena cava (SVC) syndrome. The aims of this prospective study were to assess the diagnostic yield and safety of these investigations and specifically to evaluate the role of ROSE in limiting the need for tissue biopsies. Over a 5‐year period 48 patients (57.4 ± 9.7 years) with SVC syndrome secondary to intrathoracic tumors underwent flexible bronchoscopy with TBNA and ROSE. Endobronchial Forceps biopsy was reserved for visible endobronchial tumors with no on‐site confirmation of diagnostic material. ROSE confirmed diagnostic material in 41 cases (85.4%), and in only one of the remaining cases did the addition of a forceps biopsy increase the diagnost... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424285 Fri, 18 Nov 2011 05:00:00 +0100 5424285 http://www.medworm.com/index.php?rid=5424284&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21856 AbstractHerein, we described a case of a 29‐year‐old female with a large ovarian mass. Fine needle aspiration cytology (FNAC) of the mass showed discrete round to oval cells in a fatty vacuolated background. FNAC diagnosis of dysgerminoma was suggested. The histology of the tumors showed features of poorly differentiated Sertoli–Leydig cell tumors. We discussed the diagnostic pitfalls of this case on FNAC. Diagn. Cytopathol. 2011. © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424284 Fri, 18 Nov 2011 05:00:00 +0100 5424284 http://www.medworm.com/index.php?rid=5424283&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21855 We report the cytologic findings of a recurrent chordoma sampled through transoral FNA from the parapharyngeal space of a 66‐year‐old woman. As the prior history of chordoma was not available during the rapid onsite evaluation, the presence of bland epithelioid nonvacuolated cells and spindle cells intimately admixed with a fibrillary, intensely metachromatic material led to an initial diagnosis of pleomorphic adenoma. Review of the patient's prior pathology specimen and of the Papanicolaou‐stained smears and cellblock sections showing rare multivacuolated (physalipherous) cells led to the correct diagnosis, which was supported by immunoperoxidase stains (cytokeratin AE1/AE3+, S100+, GFAP−). A review of the literature found no previous instances in which chordomas mimicked pleomorp... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424283 Fri, 18 Nov 2011 05:00:00 +0100 5424283 http://www.medworm.com/index.php?rid=5424282&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21854 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424282 Fri, 18 Nov 2011 05:00:00 +0100 5424282 http://www.medworm.com/index.php?rid=5424281&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21853 In this study, we report the fine‐needle aspiration (FNA) findings of 16 basaloid squamous cell carcinoma cases and compare those cases to 16 cases of small cell carcinoma. To our knowledge, this is the largest series of basaloid squamous cell carcinoma FNA cases ever reported. The following cytomorphologic features were compared for both tumors: cohesive tissue fragments, single cells, adenoid cystic‐like features (cribriform pseudoglandular lumina with hyaline materials), necrosis, nuclear size, nuclear molding, nucleoli, cytoplasm, and the presence of single keratinized cells. Adenoid cystic‐like features and the presence of single keratinized cells were specific for basaloid squamous cell carcinoma (P < 0.05). Diagn. Cytopathol. 2011; © 2011 Wiley Periodicals, Inc. (Source: D... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424281 Fri, 18 Nov 2011 05:00:00 +0100 5424281 http://www.medworm.com/index.php?rid=5424280&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21852 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424280 Fri, 18 Nov 2011 05:00:00 +0100 5424280 http://www.medworm.com/index.php?rid=5424279&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21851 In this study, we report our institutional experience with cytology cell block material for EGFR mutation testing. We retrospectively reviewed EGFR mutation analyses performed on 234 surgical (SP) and cytology (CB) from October 2007 to May 2010. One hundred ninety‐two SP specimens and 42 CB specimens were evaluated for EGFR mutation. CB specimens were evaluated for overall specimen size based on aggregate cellularity in comparison to small biopsy specimens, and percent tumor. Of the 192 SP and 42 CB specimens, 31 (16.1%) and 11 (26.2%) were positive for EGFR mutation, respectively; there does not appear to be an association between mutation detection rate and the source of the specimen (P = 0.124). Limited DNA was obtained from 70.0% (29/42), including 81.8% (9/11) of those which were mu... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424279 Fri, 18 Nov 2011 05:00:00 +0100 5424279 http://www.medworm.com/index.php?rid=5424278&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21850 AbstractSerum calcitonin is a tumor marker used in the diagnosis and follow‐up of medullary thyroid carcinoma. Calcitonin washout evaluation is a new method used for suspicious thyroid nodules and lymph nodes. Limited clinical data are present about the efficacy of this method. A 61‐year‐old female patient with known Hashimoto's thyroditis and an 8‐mm hypoechoic nodule was presented with one previously benign fine‐needle aspiration cytology (FNAC). On referral to our department, she had a moderately high‐serum calcitonin level, and we repeated the FNAC that was reported as nondiagnostic. We performed FNAC for the third time together with calcitonin washout evaluation from the thyroid nodule. The FNAC was again nondiagnostic, but the calcitonin washout level from the thyroid nod... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5424278 Fri, 18 Nov 2011 05:00:00 +0100 5424278 http://www.medworm.com/index.php?rid=5411558&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21725 AbstractTumor cytology has proven to be inadequate for precise diagnosis of thyroid follicular adenoma. This suggests the need for a molecular approach for its diagnosis.Expression of CD26/DPPIV (dipeptidyl peptidas IV), p53, and PTEN was analyzed in smears or sections obtained from 19 patients with histologically proven thyroid follicular adenoma. Papanicolaou staining, CD26/DPPIV activity staining, and HE staining were performed and the specimens were observed morphologically. Immunohistochemical analysis using antibodies against p53 and PTEN was performed. Genetic mutation of PTEN exons was performed using the laser capture microdissection method.The nuclear area of the CD26/DPPIV‐positive cells was significantly larger than that of the CD26/DPPIV‐negative cells. p53 expression was ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5411558 Thu, 17 Nov 2011 08:19:35 +0100 5411558 http://www.medworm.com/index.php?rid=5366941&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21848 In conclusion, the SP + FP detected 100% of HG abnormalities in the trial set; significantly reduced the rate of unsatisfactory specimens; and improved the overall screening rate of detection of HG abnormalities particularly of glandular lesions when compared with other screening technologies. Diagn. Cytopathol. 2011;. © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366941 Tue, 01 Nov 2011 04:00:00 +0100 5366941 http://www.medworm.com/index.php?rid=5366940&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21847 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366940 Tue, 01 Nov 2011 04:00:00 +0100 5366940 http://www.medworm.com/index.php?rid=5366939&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21846 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366939 Tue, 01 Nov 2011 04:00:00 +0100 5366939 http://www.medworm.com/index.php?rid=5366938&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21845 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366938 Tue, 01 Nov 2011 04:00:00 +0100 5366938 http://www.medworm.com/index.php?rid=5366937&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21844 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366937 Tue, 01 Nov 2011 04:00:00 +0100 5366937 http://www.medworm.com/index.php?rid=5366936&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21842 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366936 Tue, 01 Nov 2011 04:00:00 +0100 5366936 http://www.medworm.com/index.php?rid=5366935&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21841 In this study, expression of ER, PR, and HER2 was assessed by immunohistochemical study in 70 cases of metastatic breast carcinomas and HER2 gene amplification was further evaluated by fluorescence in situ hybridization (FISH) in 38 (54%) cases. Positive expression of ER and PR was seen in 42 (60%) and 16 (23%) cases of metastatic breast carcinomas, respectively. HER2 immunoreactivity was scored as 0/1+ in 39 (56%), 2+ in 10 (14%), and 3+ in 21 (30%) cases. HER2 gene amplification was seen in 20% of HER2 2+ and 64% of HER2 3+ cases. ER, PR, and HER2 status in primary breast cancers were available to comparison in 31 cases (44%). The concordance rates between metastatic and primary breast carcinomas were 81% for ER, 65% for PR and 71% for HER2. Our study demonstrates that ER, PR, and HER2 s... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366935 Tue, 01 Nov 2011 04:00:00 +0100 5366935 http://www.medworm.com/index.php?rid=5366934&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21835 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366934 Tue, 01 Nov 2011 04:00:00 +0100 5366934 http://www.medworm.com/index.php?rid=5366933&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21832 We report five cases of intrapancreatic splenules and one case of accessory spleen (AS) in which a definitive diagnosis was made on EUS‐FNA. Previously recognized FNA cytomorphologic features of splenic tissue, including ASs and splenosis, are endothelial cells and polymorphous lymphocytes admixed with neutrophils, eosinophils, plasma cells, histiocytes, and lymphoglandular bodies. We describe the additional finding of abundant large platelet aggregates as another distinguishing feature of splenic tissue on FNA. In all six cases, large platelet aggregates were identified along with polymorphous lymphoid cells, lymphoglandular bodies, loose aggregates of endothelial cells and scattered or aggregated bland spindle cells. A review of 10 consecutive cases of EUS‐FNA‐sampled benign intraa... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366933 Tue, 01 Nov 2011 04:00:00 +0100 5366933 http://www.medworm.com/index.php?rid=5366932&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21830 This report characterizes the cytomorphologic features of five cases of AFH, with correlation to clinical, histology, and cytogenetic findings.Smears of fine needle aspiration (FNA; four cases) and intraoperative scrape (one case) were reviewed from five patients with a histologically confirmed diagnosis of AFH. A review of six previously reported AFH cases with cytomorphology was also performed.The tumor presented as a cystic, deep dermal mass in three pediatric cases and as a solid, deeply seated mass in two adults. The cytomorphologic features are mostly nondistinctive and include cellular smears with ovoid to spindled histiocytoid cells that may be isolated or in clusters. Some of these cells are atypical and others contain hemosiderin. Large cellular clusters with a capillary structur... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5366932 Tue, 01 Nov 2011 04:00:00 +0100 5366932 http://www.medworm.com/index.php?rid=5350371&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21776 AbstractNo absract. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350371 Thu, 27 Oct 2011 05:42:40 +0100 5350371 http://www.medworm.com/index.php?rid=5350378&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21826 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350378 Mon, 24 Oct 2011 04:00:00 +0100 5350378 http://www.medworm.com/index.php?rid=5350377&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21824 We present a case of a 67‐year‐old female with lymphohistiocytoid mesothelioma involving the left pleura. The cytological, histological, and immunohistochemical features are discussed. © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350377 Mon, 24 Oct 2011 04:00:00 +0100 5350377 http://www.medworm.com/index.php?rid=5350376&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21801 We report a case of a 44‐year‐old man who complained for a slowly growing, asymptomatic mass in the left parotid gland that since 12 months. Fine needle aspiration biopsy (FNAB) showed a loose collections of bland‐appearing spindle cells in a myxoid background admixed with capillary fragments and some mature fat cells suggesting a diagnosis of SCL. A cytological diagnosis of mesenchymal myxoid spindle cell tumor with lipomatous differentiation, possibly an intraparotideal SCL was performed. Histological examination of the mass and the positive immunostaining for CD34 and negativity for S‐100, CK‐cocktail, and actin confirmed the diagnosis of SCL. The diagnosis of intraparotid SCL can be made by examining cytologic material containing mature fat with bland spindle cells in a myxoi... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350376 Mon, 24 Oct 2011 04:00:00 +0100 5350376 http://www.medworm.com/index.php?rid=5350375&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21792 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350375 Mon, 24 Oct 2011 04:00:00 +0100 5350375 http://www.medworm.com/index.php?rid=5350374&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21791 In conclusion, by blocking endogenous biotin, Melan‐A antibody offers the greatest performance. In terms of cost‐effectiveness, we suggested that Melan‐A antibody should be used as the first‐line antibody for detecting melanoma in the cytologic specimens. © 2011 Wiley Periodicals, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350374 Mon, 24 Oct 2011 04:00:00 +0100 5350374 http://www.medworm.com/index.php?rid=5350373&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21787 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350373 Mon, 24 Oct 2011 04:00:00 +0100 5350373 http://www.medworm.com/index.php?rid=5350372&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21786 AbstractVaginal lactobacilli assessed by PCR‐based microarray and PCR‐based genotyping of HPV in South African women at risk for HIV and BV.Vaginal lactobacilli can be defined by microarray techniques in fixed cervical samples of South African women. Cervical brush samples suspended in the coagulant fixative BoonFix of one hundred women attending a health centre for HIV testing in South Africa were available for this study. In the Ndlovu Medical Centre in Elandsdoorn, South Africa, identification of 18 hr‐HPV genotypes was done using the INNO‐LiPA method. An inventory of lactobacilli organisms was performed using microarray technology.On the basis of the Lactobacillus and Lactobacillus biofilm scoring, the cases were identified as Leiden bacterial vaginosis (BV) negative (BV‐; n ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5350372 Mon, 24 Oct 2011 04:00:00 +0100 5350372 http://www.medworm.com/index.php?rid=5306552&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21777 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306552 Tue, 11 Oct 2011 04:00:00 +0100 5306552 http://www.medworm.com/index.php?rid=5306551&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21827 We report here the cytopathological features of CLCC of the lung with an emphasis on differential diagnostic considerations. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306551 Tue, 11 Oct 2011 04:00:00 +0100 5306551 http://www.medworm.com/index.php?rid=5306550&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21836 In this study, spontaneously occurring MN were counted in epithelial cells on fine needle aspiration cytology (FNAC) smears from 50 patients with benign and malignant breast lesions. Further, the ploidy status and S‐phase fraction (SPF) of the samples was determined by flow cytometry. All these were then correlated with grades of breast cancer at cytology. Most IDC cases showed variable number of MN (n = 16, MN mean = 9.3), in contrast to the benign lesions (n = 26) where they were consistently absent. Aneuploidy and SPF analysis also showed a significant difference between benign (n = 10, mean DNA index [DI] = 0.96 ± 0.04, mean SPF= 8.07% ± 2.93) and malignant (n = 10, mean DI = 1.5 ± 0.41, mean SPF = 25.05% ± 10.35) lesions. On statistical analysis, a positive correlation was obser... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306550 Tue, 11 Oct 2011 04:00:00 +0100 5306550 http://www.medworm.com/index.php?rid=5306549&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21834 We report the case of a 59‐year‐old Japanese woman with cervical LCNEC combined with small cell carcinoma (SmCC). Cytologic specimens from the uterine cervix demonstrated large cells with coarse chromatin and prominent nucleoli. Frequent mitotic figures were also observed. Curettage of the uterine endometrium revealed an endometrioid adenocarcinoma with squamous differentiation; i.e., an adenoacanthoma. Histologic examination of surgically resected uterine cervical tissue revealed LCNEC with minor foci of SmCC. Neuroendocrine differentiation in LCNEC was confirmed by immunohistochemistry for synaptophysin and CD56. Cytotechnologists or pathologists need to consider a differential diagnosis of LCNEC while examining cervical cytologic specimens; therefore, it is important to correctly id... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306549 Tue, 11 Oct 2011 04:00:00 +0100 5306549 http://www.medworm.com/index.php?rid=5306548&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21831 In this study, a cell with four signals for each probe was classified as a uniform tetraploid cell (UTC); a presumed reparative cell which is currently classified as an abnormal cell in the FDA approved assay. By using the existing criteria, the tests were scored as positive or negative before and after exclusion of the UTCs. Before the exclusion, 24 positive, 13 negative, seven false positive, and one false negative result were obtained with 96% sensitivity and 65% specificity. After the exclusion, the results changed to 22 positive, 19 negative, one false positive, and three false negatives resulting in a 88% sensitivity of 88% and a 95% specificity; a significant improvement in the specificity. We conclude that exclusion of the UTCs as abnormal cells would result in a more solid perform... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306548 Tue, 11 Oct 2011 04:00:00 +0100 5306548 http://www.medworm.com/index.php?rid=5306547&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21814 We present here an unusual case of multiple diffuse cutaneous nodular lesions predominantly in face, trunk, and upper extremities diagnosed initially on aspiration cytology as histoplasmosis. Subsequent serological test revealed positivity for HIV 1 and 2, along with a low CD4 count and low CD4:CD3 ratio. The cytomorphological features were further corroborated by histology and histochemical stains. Hence, cutaneous histoplasmosis can cause multiple wide spread nodular or umbilicated lesions in AIDS patients as the initial presentation. Fine needle aspiration cytology (FNAC) is a rapid, cost effective tool for diagnosis of the fungi from such lesions and initiating work up for immunocompromised states including AIDS. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic C... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306547 Tue, 11 Oct 2011 04:00:00 +0100 5306547 http://www.medworm.com/index.php?rid=5306546&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21807 AbstractInvasive breast cancer is divided into luminal A, luminal B, HER2 overexpression, basal‐like (BL) and normal‐like subtypes, among which the BL subtype has the worst prognosis. The purpose of this study was to determine the clinicopathological and cytological characteristics of BL breast cancer (BLBC). Fine‐needle aspiration cytology samples from 17 patients with consecutive BLBC were investigated, and the findings were compared with those of other subtypes (10 cases each) for the following cytomorphological features: necrosis; lymphocyte infiltration; mitotic index; apoptosis; naked nuclei; nuclear/cytoplasmic ratio; nuclear margin, size and pleomorphism; chromatin granularity and density; and nucleolar appearance. Histologically, the BLBCs were heterogeneous, and included me... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306546 Tue, 11 Oct 2011 04:00:00 +0100 5306546 http://www.medworm.com/index.php?rid=5306545&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21781 We report one such case with some overlapping cytomorphological features with chronic lymphocytic leukemia and acute lymphoblastic leukemia. A 91‐year‐old man presented with generalized lymphadenopathy, pleural effusion, ascites, and an ulcerated growth in rectum. FNA smears from the left cervical lymph node showed a monotonous population of small lymphoid cells having small but distinct nucleoli that was initially diagnosed as chronic lymphocytic leukemia (CLL). Smears from the left axillary lymph node contained both small and medium‐sized lymphoid cells with frequent hand‐mirror cell appearance, which has been described in acute lymphoblatic leukemia (ALL). Immunocyto/histochemical stainings on smears and cell block preparations of the aspirate showed the following immunophenotyp... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306545 Tue, 11 Oct 2011 04:00:00 +0100 5306545 http://www.medworm.com/index.php?rid=5306544&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21780 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306544 Tue, 11 Oct 2011 04:00:00 +0100 5306544 http://www.medworm.com/index.php?rid=5306543&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21779 We present 10 cases of abdominal wall endometriosis diagnosed by fine‐needle aspiration (FNA) cytology and confirmed by subsequent surgical excision. A palpable abdominal wall mass was the most common symptom, followed by pain. Nine cases were associated with previous surgery. The smear showed glandular epithelial cells and spindle or ovoid stromal cells accompanied by macrophages in eight cases. The nucleus was round‐to‐oval and the nucleolus was inconspicuous. Mitosis was not observed in any case. In two cases, which were suspicious of malignancy on FNA, the epithelial cells showed anisonucleosis, hyperchromasia, and small conspicuous nucleoli. The stromal cells were spindle or ovoid in shape, without metaplastic changes. Histiocytes were noted in nine cases and hemosiderin‐laden... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306543 Tue, 11 Oct 2011 04:00:00 +0100 5306543 http://www.medworm.com/index.php?rid=5306542&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21778 This study investigates the distribution of human papillomavirus (HPV) in women with abnormal cervical cytology in Kuwait. Two hundred and ninety‐eight (298) abnormal ThinPreps were taken from women seeking routine gynecological care and screened for HPV DNA by real‐time PCR. HPV genotyping was determined by PCR‐based sequencing. HPV DNA was detected in 152 women (51%), and 29 different HPV genotypes were detected, comprising 16 high‐risk (HR) (16, 18, 31, 33, 35, 39, 45, 51, 53, 56, 58, 59, 66, 68, 73, 97), nine low‐risk (LR) (6, 11, 54, 61, 74, 81, 90, 102, 106), and four intermediate‐risk (IR) (62, 67, 84, 87). HPV16 had the highest prevalence (24.3%), followed by HPV11 (13.8%), HPV66 (11.2%), HPV33 (9.9%), HPV53 (9.2%), HPV81 (9.2%), HPV56 (7.9%) and HPV18 (6.6%). HPV preva... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306542 Tue, 11 Oct 2011 04:00:00 +0100 5306542 http://www.medworm.com/index.php?rid=5306541&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21773 In this study, we applied artificial neural network (ANN) for the diagnosis of lobular carcinoma in fine‐needle aspiration cytology (FNAC) material. We selected a total of 64 cases of histology proven breast lesions consisting of 20 fibroadenomas, 28 infiltrating ductal carcinomas (IDC), and 16 infiltrating lobular carcinomas (ILC). Detailed cytomorphological features were studied on representative Haematoxylin–Eosin (H&E) and May‐Grunwald Giemsa stained slides. Image morphometric analysis was performed on Haematoxylin–Eosin stained smears to study nuclear area, diameter, perimeter, roundness, convex area, and convex perimeter. Both the qualitative cytological features and objective morphometric data were collected and a total of 18 variables were studied. Back propagation ANN ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306541 Tue, 11 Oct 2011 04:00:00 +0100 5306541 http://www.medworm.com/index.php?rid=5306540&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21770 We report a case of pulmonary small‐cell carcinoma occurring in an 88‐year‐old woman that initially presented with a malignant pleural effusion characterized cytologically by a predominance of large three‐dimensional neoplastic cell aggregates (“cannonballs”), associated with rare psammoma bodies. Although the crowded three‐dimensional tumor‐cell aggregates did not allow detailed cytologic examination, the diagnosis of metastatic small‐cell carcinoma could be established noting the characteristic chromatin features of the occasional single neoplastic cells and the characteristic “cell‐in‐cell” and “stack‐of‐coins” arrangements of rare small clusters of neoplastic cells. Immunoperoxidase stains showing positivity of the tumor cells for CD56, synaptophysin, ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306540 Tue, 11 Oct 2011 04:00:00 +0100 5306540 http://www.medworm.com/index.php?rid=5306539&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21774 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5306539 Sat, 01 Oct 2011 04:00:00 +0100 5306539 http://www.medworm.com/index.php?rid=5276567&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21825 AbstractThe aims of this study were to determine the expression of Ki‐67 in type I and type II endometrial adenocarcinomas as well as normal endometrium in imprint smears and to correlate the results with clinicopathologic parameters of primary untreated endometrial cancer patients. During a 29‐month period, 255 patients were evaluated with entometrial imprint cytology. Endometrial samples freshly resected from women who underwent total abdominal hysterectomy were studied. One hundred twenty‐six patients had endometrial carcinoma and 129 cases were diagnosed as normal endometrium. The expression of Ki‐67 was assessed by immunocytochemistry. Positive staining was correlated with increased stage, grade and lymph node metastases. High expression was more frequent in type II than type ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276567 Sat, 01 Oct 2011 04:00:00 +0100 5276567 http://www.medworm.com/index.php?rid=5276576&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21823 AbstractAmeloblastoma is an odontogenic tumor with aggressive biological behavior, high recurrence rate, and a complex microscopic appearance with many different histologic patterns. Primary ameloblastoma is also described in extragnathic locations. Because of its wide morphologic spectrum, which is mirrored also in cytologic smears, a thorough study of distinctive features is required to reach a reliable diagnosis. Twelve cases of ameloblastoma were examined both cytologically and histologically. The patients were seven women and five men 24–85 years old, mean age being 64 years. Eleven cases were primary tumors of the mandible and maxilla, and one case was a lung tumor metastatic from the tibia. The epithelial element in the cytologic smears of the various cases was morphologically div... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276576 Fri, 30 Sep 2011 04:00:00 +0100 5276576 http://www.medworm.com/index.php?rid=5276575&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21822 AbstractStrongyloidiasis is an infectious disease caused by the nematode helminth, Strogyloides stercoralis. Subjects immunosuppressed, immunocomprised, or on steroids are susceptible to hyperinfection and dissemination. Early detection of parasitic infection in such individuals is extremely important as disseminated Strongyloidiasis is potentially fatal. Here, we present a case of Strongyloidiasis initially detected by Prussian blue stain of bronchoalveolar lavage (BAL) specimen from an immunosuppressed patient who was treated with prednisone for acute interstitial nephritis and developed hemoptysis. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276575 Fri, 30 Sep 2011 04:00:00 +0100 5276575 http://www.medworm.com/index.php?rid=5276574&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21821 In this study, the expression of metadherin (MTDH) and erythropoietin‐producing hepatoma‐amplified sequence (Eph) receptor A7 (EphA7) in 96 benign and 108 malignant lesions of gallbladder was determined by immunohistochemistry, and their correlations with pathological features and prognosis were analyzed. Positive expression of EphA7 and MTDH was significantly higher in gallbladder adenocarcinoma than in benign lesions. In adenocarcinoma, the positive expression of EphA7 and MTDH was significantly associated with differentiation, tumor mass, lymphnode metastasis, invasion, and overall survival. Multivariate Cox regression analysis suggested that positive expression of EphA7 and MTDH was an independent poor‐prognostic predictor in gallbladder adenocarcinoma. The elevated expression of... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276574 Fri, 30 Sep 2011 04:00:00 +0100 5276574 http://www.medworm.com/index.php?rid=5276573&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21820 AbstractInterinstitutional consultation in pathology has shown to improve patient safety by detecting interpretive errors that may significantly impact clinical management. We conducted a study of 922 cases of thyroid FNAC slides, referred to our institution over a 2‐year period, to assess the magnitude of discrepancies and determine the clinical impact of second opinion. Disagreements were categorized as none, minor or major, the latter two defined as one‐ or two‐step deviations respectively on the NCI diagnostic categories scale. There were 122 disagreements (13%), including 44 major and 78 minor. Seventy‐five patients underwent a change in management based on second opinion, in conjunction with clinical and radiologic findings (age, size of nodule, family history, ultrasonograph... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276573 Fri, 30 Sep 2011 04:00:00 +0100 5276573 http://www.medworm.com/index.php?rid=5276572&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21819 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276572 Fri, 30 Sep 2011 04:00:00 +0100 5276572 http://www.medworm.com/index.php?rid=5276571&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21839 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276571 Fri, 30 Sep 2011 04:00:00 +0100 5276571 http://www.medworm.com/index.php?rid=5276570&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21838 We report a 65‐year‐old male patient of HCC who presented with bone and soft tissue metastases and was clinically and radiologically suspected to have a soft tissue sarcoma. The patient presented with severe cervical pain with palpable masses in right scapular, nape of neck, and occiput area of scalp. Radiologically, these were large, bulky soft tissue masses expansile, destructive, and lytic in nature. Cytomorphologic studies revealed HCC with uncommon features of multinucleated osteoclast‐like giant cell and very prominent intracytoplasmic hyaline bodies (IHBs). Cytology, immunohistochemistry on cell block preparation, rising serum α‐fetoprotein (AFP) levels (1121.93–5000 ng/ml), and PIVKA II levels confirmed the diagnosis. The patient has been on follow‐up on sorafinib for ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276570 Fri, 30 Sep 2011 04:00:00 +0100 5276570 http://www.medworm.com/index.php?rid=5276569&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21829 AbstractThe diagnosis of nodular sclerosing classical Hodgkin lymphoma (NSCHL) by fine‐needle aspiration (FNA) biopsy has historically been a diagnostic challenge due to the usual paucicellularity of the specimen. This case report, and other previously published reports, suggests that there is another facet to the potentially challenging diagnosis of this particular variant of Hodgkin lymphoma (HL): the presence of suppurative‐necrotizing changes mimicking an infectious etiology. The patient presented here underwent FNA biopsy of an acutely enlarged supraclavicular lymph node and cytologic smears showed marked acute inflammation in a background of necrosis. A diagnosis of infectious suppurative lymphadenitis was made at that time. After a negative infectious work‐up with infectious d... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276569 Fri, 30 Sep 2011 04:00:00 +0100 5276569 http://www.medworm.com/index.php?rid=5276568&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21828 AbstractFine‐needle aspiration (FNA) diagnosis of oncocytic lesions is challenging. In fact, oncocytic changes occur in inflammatory, hyperplastic, and neoplastic settings, including both benign and malignant tumors. The rare oncocytic variant of papillary thyroid carcinoma (PTC), shows papillae composed by cells with large oncocytic granular cytoplasm featuring clear PTC nuclear features. A morphological similar, but biologically distinct lesion, is the encapsulated papillary oncocytic neoplasia. Here, we first report on FNA, its cytological features together with histological, immunohistochemical, and molecular correlates. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5276568 Fri, 30 Sep 2011 04:00:00 +0100 5276568 http://www.medworm.com/index.php?rid=5263358&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21818 We present a rare occurrence of metastatic proximal epithelioid sarcoma (PES) in the pleural effusion of a 23‐year‐old man, developed within one year of diagnosis in his gluteal soft tissue. The cytologic and immunoperoxidase findings are described. PES, due to its epithelioid morphology, can be confused with more common cancers in effusions such as adenocarcinoma and mesothelioma. PES is an aggressive neoplasm that differs clinically and pathologically from conventional epithelioid sarcoma. Knowledge of its cytomorphology in serous cavity effusions, a patient's clinical history and ancillary studies may lead to an accurate diagnosis. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263358 Thu, 29 Sep 2011 18:07:32 +0100 5263358 http://www.medworm.com/index.php?rid=5263367&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21840 AbstractAlthough uncommon, Hurthle cell neoplasms have a characteristic histologic and cytologic features which often aid in the diagnosis of these lesions. In fine needle aspiration biopsies, determining malignant potential on cytologic features is challenging unless other evidence of malignancy (such as metastases) are known. The role of EUS‐guided FNA in diagnosing superior mediastinal masses has been described previously. It's role in evaluating subcarinal metastases from lung cancer primaries have also heen studied. However we describe metastatic thyroid Hurthle cell carcinoma to the subcarina diagnosed through EUS‐guided FNA combining the unique cytologic features o Hurthle cell neoplasms and the versatility ofthe endoscopic ultrasound to reach a diagnosis in this unusual neoplas... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263367 Mon, 26 Sep 2011 04:00:00 +0100 5263367 http://www.medworm.com/index.php?rid=5263366&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21837 AbstractIt is well known that “condensed cluster of stromal cells (CCSC)” and “metaplastic clumps with irregular protrusion (MCIP)” in endometrial glandular and stromal breakdown (EGBD) cases may simulate “clumps of cancer cells (CCC)” in endometrioid adenocarcinoma grade 1 (G1), leading to difficulty in cytological interpretation. The aim of this study was undertaken to clarify the cytological immunoreactivity of nuclear findings about CCSC and MCIP which may be recognized in EGBD cases by using p53 protein and cyclin A in liquid‐based cytologic (LBC) preparations. The material consists of cytologic smears of 20 cases of EGBD and 20 cases of G1 for which histopathological diagnosis was obtained by endometrial curettage at the JA Suzuka General Hospital. The evaluation of imm... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263366 Mon, 26 Sep 2011 04:00:00 +0100 5263366 http://www.medworm.com/index.php?rid=5263365&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21833 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263365 Mon, 26 Sep 2011 04:00:00 +0100 5263365 http://www.medworm.com/index.php?rid=5263364&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21817 In this study, cytology, histopathology, and radiological imaging were employed in order to reach a diagnosis of sarcomatoid carcinoma. CT imaging showed increased thickness of the bladder wall associated to a polypoid mass. MRI showed a 4‐cm sized, broadly necked polypoid mass with calcification and ulceration at the right side of the bladder wall. T2W1 imaging showed low signal. Voided urinary cytology showed a scattered cellular presentation. The tumor cells had a high nucleo‐ cytoplasmic ratio, with elongated cytoplasm with faint with indistinct cytoplasm border. The nucleus was oval to round, with large and irregular nucleoli and irregular nuclear membrane. These tumor cells were positive for cytokeratin (CKAE1/AE3), vimentin, p53, carcinoembryonic antigen (CEA), α1‐smooth musc... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263364 Mon, 26 Sep 2011 04:00:00 +0100 5263364 http://www.medworm.com/index.php?rid=5263363&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21816 AbstractMalignant lymphomas of the female genital tract are very uncommon, and the development of a diffuse large B‐cell lymphoma involving the vagina following treatment for classic Hodgkin lymphoma is extremely rare. Clinically and morphologically, this entity represents a challenge. We herein report such a case with liquid‐based Pap test and tissue biopsy findings. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263363 Mon, 26 Sep 2011 04:00:00 +0100 5263363 http://www.medworm.com/index.php?rid=5263362&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21813 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263362 Mon, 26 Sep 2011 04:00:00 +0100 5263362 http://www.medworm.com/index.php?rid=5263361&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21812 AbstractIndications of fine‐needle aspiration (FNA) of spleen have increased as more splenic lesions are detected because of advanced imaging techniques. A retrospective analysis of cytological material of 36 patients on whom ultrasound‐guided splenic FNA was performed was done. No complications were noted. There were 16 inflammatory lesions, 12 neoplastic and 8 cases were reported as descriptive either because of scant cellularity, blood only, or normal splenic cytology. Inflammatory lesions included nine cases of acute abscess, five cases of tuberculosis, and one case each of leishmaniasis and infarct. Neoplastic lesions included two benign (benign cyst and inflammatory pseudotumor) and 10 malignant lesions. Among malignant lesions, eight were non‐Hodgkin lymphoma (NHL), one suspic... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263361 Mon, 26 Sep 2011 04:00:00 +0100 5263361 http://www.medworm.com/index.php?rid=5263360&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21811 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263360 Mon, 26 Sep 2011 04:00:00 +0100 5263360 http://www.medworm.com/index.php?rid=5263359&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21810 AbstractKikuchi‐Fujimoto disease (KFD) is cytologically characterized by a polymorphous lymphoid cell population, abundant karyorrhectic debris and histiocytes, many of which are crescentic (Kikuchi histiocytes). As per reviewed literature, KFD may be confused with tuberculosis, lymphoma, and reactive hyperplasia of lymph nodes (RHLN). Since RHLN was found to be a major challenging factor during routine cytodiagnosis of KFD in our material, we tried to find out the differentiating clinico‐cytologic features between 76 KFD and 684 RHLN cases seen in Kuwait. 63.2% of KFD were in 3rd and 4th decades of life as compared to 40.2% of RHLN (P = 0.0002). Male to female ratio was 1: 2.45 for KFD and 1:1.09 for RHLN (P = 0.0022). Kuwaiti:non‐Kuwaiti ratio was 1:2.04 for KFD and 1.31:1 for RHLN... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5263359 Mon, 26 Sep 2011 04:00:00 +0100 5263359 http://www.medworm.com/index.php?rid=5240982&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21809 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240982 Thu, 22 Sep 2011 17:59:33 +0100 5240982 http://www.medworm.com/index.php?rid=5240991&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21804 The objective of this study is to assess the utility of endoscopic ultrasound‐guided fine‐needle aspiration (EUS‐FNA) in the diagnosis and staging of colorectal cancer. The study includes patients who underwent EUS‐FNA at our institution for staging of colorectal carcinoma or for evaluation peri‐rectal masses or distal metastases from August 2000 to November 2010. We assessed the frequency with which EUS‐FNA procedure confirms the diagnosis of malignancy and the percent of cases in which it modifies staging of colorectal carcinoma. Using histology as a reference standard, we also assessed the diagnostic performance. We identified 79 cases of EUS‐FNA from 77 patients, mean (SD) age of 60 (12.5), 44 males. Twenty‐seven (34%) aspirates were from patients with primary rectal/pe... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240991 Mon, 19 Sep 2011 04:00:00 +0100 5240991 http://www.medworm.com/index.php?rid=5240990&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21803 AbstractHistoplasmosis has emerged as an important opportunistic fungal infection in immunocompromised patients. Histoplasma is a dimorphic fungus that primarily involves lung and the environmental reservoir is soil. Although several cases of histoplasmosis have been reported in India but cytological diagnosis was made in a few cases. We are presenting two cases of histoplasmosis diagnosed on fine‐needle aspiration cytology. In the first case, pulmonary histoplasmosis was diagnosed on transbronchial needle aspiration of lung in a 41‐year‐old immunocompetent male, while second case was of disseminated histoplasmosis in 40‐year‐old immunocompromised female diagnosed on cytology of cervical lymph node. FNAC is a simple, safe, and rapid technique to establish the initial diagnosis, t... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240990 Mon, 19 Sep 2011 04:00:00 +0100 5240990 http://www.medworm.com/index.php?rid=5240989&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21808 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240989 Mon, 19 Sep 2011 04:00:00 +0100 5240989 http://www.medworm.com/index.php?rid=5240988&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21802 AbstractFine‐needle aspiration (FNA) is a screening and diagnostic tool used to triage the management of thyroid nodules. While FNA has proved to be a sensitive means of detecting common thyroid malignancies, less is known about the sensitivity and positive predictive value (PPV) of FNA for uncommon thyroid malignancies, including anaplastic thyroid carcinomas, medullary thyroid carcinomas, lymphomas, metastatic carcinomas, and other malignancies. We reviewed our experience with these uncommon malignancies sampled by thyroid FNA and recorded interpretations according to the Bethesda System. We compared the FNA interpretations to the follow‐up cytology, histology, and flow cytometry. The sensitivity and PPV were as follows: anaplastic thyroid carcinoma (sensitivity 100%, PPV 89%), lymph... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240988 Mon, 19 Sep 2011 04:00:00 +0100 5240988 http://www.medworm.com/index.php?rid=5240987&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21794 AbstractLiposarcoma (LS) represents the most common type of soft tissue sarcoma. LS is subdivided into four distinct subtypes: well‐differentiated, myxoid/round cell, pleomorphic, and mixed LS. In addition to distinctive morphology, each of the subgroups has a different prognosis and treatment strategy. While subgrouping has traditionally been based on conventional morphology, recent molecular and cytogenetic findings have validated the current classification system. Well‐differentiated LS is characterized by overexpression of MDM2, which blocks the tumor suppressor function of p53. Myxoid/round cell LS is characterized by a specific translocation resulting in a fusion protein which interferes with adipocyte differentiation. Pleomorphic LS, like other high grade sarcomas, is characteri... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240987 Mon, 19 Sep 2011 04:00:00 +0100 5240987 http://www.medworm.com/index.php?rid=5240986&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21793 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240986 Mon, 19 Sep 2011 04:00:00 +0100 5240986 http://www.medworm.com/index.php?rid=5240985&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21785 AbstractErythema nodosum leprosum (ENL) or type 2 lepra reaction is an inflammatory reaction, which may occur in the course of hanseniasis, may compel the patient to seek medical attention and may result in nerve function impairment and subsequent disability. Thus, recognition and timely management of these patients is critical in order to avoid permanent disability. Fine‐needle aspiration cytology is simple and effective tool that aids in the correct diagnosis and management of ENL. Herein, we present two cases of ENL, one with typical and another with atypical presentation. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240985 Mon, 19 Sep 2011 04:00:00 +0100 5240985 http://www.medworm.com/index.php?rid=5240984&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21782 AbstractThe efficacy of thyroid (FNAB) processed by liquid‐based cytology (LBC) in Hashimoto's Thyroiditis (HT) in two reference periods, is evaluated. The morphologic features of 820 cases with both methods and the cyto‐histological comparison are analyzed. The diagnosis of hyperplastic nodules (HN) in HT, its mimickers especially in presence of oxyphilic cells and the role of immunocytochemistry (IHC) are studied.150 cases of HT processed by conventional smear (CS) in 1996–98 and 670 with LBC in 2005–2007,were included. The majority of FNAB were carried out under USguidance and fixed with ethyl alcohol for the CS. LBC material was rinsed in the Cytolit solution, processed according to the manufacturer's recommendations. Among the 150 CS, 83 were HT while 67 were HN in HT; in the ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240984 Mon, 19 Sep 2011 04:00:00 +0100 5240984 http://www.medworm.com/index.php?rid=5240983&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21771 AbstractMalignant small round cell tumors represent a diagnostic challenge for cytologists and pathologists. This case series describes four cases of unusual metastasis of small round cell tumors subtypes into body cavities generating effusions in which fluid cytological examination suggested the neuroendocrine origin of the tumors. Tumor diagnosis (Ewing sarcoma/primitive neuroectodermal tumor and desmoplastic small round cell tumors) were unknown at the cytological evaluation. We can highlight the importance of the accurate analyses of body fluids, both for early diagnosis of metastatic disease, and for the diagnosis of primary tumor when serous effusion is the first manifestation of the neoplasia. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5240983 Mon, 19 Sep 2011 04:00:00 +0100 5240983 http://www.medworm.com/index.php?rid=5164992&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21764 AbstractMesoblastic nephroma (MN) is the most common renal tumor diagnosed in infancy. A case of congenital MN was diagnosed in a 6‐month old child by fine‐needle aspiration cytology. The smears were cellular and consisted of plump spindle cells arranged in clusters along with scattered naked nuclei in the background. Blastemal, epithelial, or glomeruloid structures were not seen. Considering the age and the cytomorphology, a diagnosis of cellular variant of MN was offered which was confirmed on histopathology. Unlike Wilms tumor, preoperative chemotherapy is not required for MN. Hence cytologic diagnosis is important. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164992 Sat, 27 Aug 2011 19:58:48 +0100 5164992 http://www.medworm.com/index.php?rid=5165001&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21799 AbstractA case of metastatic mixed acinar‐neuroendocrine carcinoma (MANEC) of the pancreas to the liver is reported. A diagnostic percutaneous US‐guided FNA and core biopsy of a liver nodule was performed. The FNA smears were cellular and showed neoplastic cells in clusters with acinar formation, isolated single cells, and scattered naked nuclei. The cytoplasm was finely granular. The nuclei were relatively uniform, some with speckled chromatin and prominent nucleoli. The immunohistochemistry performed on the cell block showed strong positivity for cytokeratin AE1/AE3, chromogranin, and synaptophysin. Furthermore, the tumor cells were weakly positive for α1‐antichymotrypsin. The Ki‐67 mitotic index was up to 50%. Based on the morphology and supporting immunohistochemical stains, t... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5165001 Thu, 25 Aug 2011 23:00:00 +0100 5165001 http://www.medworm.com/index.php?rid=5165000&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21798 AbstractLaboratory contamination of clinical specimens submitted for cytological examination is an on‐going threat that requires continuous vigilance and quality control. We recently were confronted with a suspected fungal infection in a bone aspirate that was found to derive from contaminated Hanks solution used in cytological preparation. Diff‐Quik staining of air‐dried cytocentrifuged preparations of Hanks fluid as well as any other suspect laboratory fluid is recommended as a routine quality control procedure. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5165000 Thu, 25 Aug 2011 23:00:00 +0100 5165000 http://www.medworm.com/index.php?rid=5164999&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21797 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164999 Thu, 25 Aug 2011 23:00:00 +0100 5164999 http://www.medworm.com/index.php?rid=5164998&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21796 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164998 Thu, 25 Aug 2011 23:00:00 +0100 5164998 http://www.medworm.com/index.php?rid=5164997&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21795 In conclusion, this case is unusual at three levels: first, extramedullary nodal BC as a presenting manifestation of CML is rare and the blasts are of precursor T lymphoblastic lineage, rather than the more common B‐cell lineage; second, this case suggests that extramedullary lymphoid nodal BC of CML can exist independently without the bone marrow developing into BC; and third, FISH analysis on the single neoplastic cell is an accurate way to confirm that the neoplasm is either extramedullary localized blasts of CML or genetically distinct neoplasm. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164997 Thu, 25 Aug 2011 23:00:00 +0100 5164997 http://www.medworm.com/index.php?rid=5164996&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21790 AbstractThe Bethesda 2007 Thyroid Cytology Classification defines follicular lesion of undetermined significance as a heterogeneous category of cases that are not convincingly benign nor sufficiently atypical for a diagnosis of follicular neoplasm or suspicious for malignancy. In our institution, we refer to these cases as indeterminate, and they are further sub‐classified into two: (1) low cellularity with predominant microfollicular architecture and absence of colloid (INa) and (2) nuclear features not characteristic of benign lesions (nuclear atypia) (INb). We reviewed these indeterminate cases to document the follow‐up trend using this two‐tier classification. A search of the cytology records was performed for the period between January 2008 and June 2009. All thyroid fine‐need... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164996 Thu, 25 Aug 2011 23:00:00 +0100 5164996 http://www.medworm.com/index.php?rid=5164995&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21788 AbstractSquamous cell carcinoma with rhabdoid features (SCCRF) is a very rare and unusual cutaneous tumor. Here, we report an extraordinary case diagnosed by fine needle aspiration biopsy, in a 66‐year‐old man, status post multiple organ transplantation. The patient presented with a large ulcerating fungating mass in his forearm that had all the light microscopic and immunohistochemical features of a SCCRF. Previously six cases of SCCRF phenotype diagnosed by surgical pathology have been reported. This is the first case diagnosed cytologically. A review of the literature with emphasis on the differential diagnoses of such unusual rhabdoid‐like tumors in fine‐needle aspiration biopsy and the potential molecular mechanism for rhabdoid phenotype in transplant patients are discussed. D... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164995 Thu, 25 Aug 2011 23:00:00 +0100 5164995 http://www.medworm.com/index.php?rid=5164994&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21800 In conclusion, a RS derived from a LR of Glut‐1 and EMA IC greatly improves the distinction between MM from RM cells in individual effusions. The study illustrates principles of evidence‐based pathology concerning internal and external test performance in the differential diagnosis of MM versus RM. Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164994 Thu, 25 Aug 2011 23:00:00 +0100 5164994 http://www.medworm.com/index.php?rid=5164993&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21784 AbstractThe aim of this study was to assess the utility of liquid‐based cytologic preparation (LP) compared with conventional preparation (CP) for the assessment of nuclear findings in endometrial glandular and stromal breakdown (EGBD) which may be misdiagnosed as carcinoma in EGBD cases. The material consists of cytologic smears including 20 cases of proliferative endometrium (PE), 20 cases of EGBD, and 20 cases of endometrioid adenocarcinoma grade1 (G1) for which histopathological diagnosis was obtained by endometrial curettage at the JA Suzuka General Hospital. Nuclear findings were examined in PE cells, EGBD‐stromal cells, EGBD‐metaplastic cells, and G1 cells, respectively. It was examined about the following items; (1) nuclear shape; (2) A long/minor axis ratio in cell nuclei; (... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=5164993 Thu, 25 Aug 2011 23:00:00 +0100 5164993 http://www.medworm.com/index.php?rid=4972027&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21748 We report the cytopathologic findings of a case of metastatic sinonasal intestinal type adenocarcinoma (ITAC) in a 58‐year‐old man who presented with enlarged cervical lymph nodes on a follow‐up imaging study, 5 months after resection of a sinonasal ITAC. Ultrasound‐guided fine needle aspiration of the station 2B lymph node yielded moderately cellular smears with abundant background mucin pools, numerous naked, atypical nuclei, and rare cells with signet ring cell morphology. Rare mitoses, apoptotic bodies, and necrotic debris were also present. Occasional clusters of signet ring cells were also seen in the cell block sections. Immunoperoxidase stains showed these cells to be positive for CK20 and villin. The differential diagnosis included a metastatic signet ring cell adenocarcin... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972027 Tue, 28 Jun 2011 01:26:41 +0100 4972027 http://www.medworm.com/index.php?rid=4972033&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21768 We report two additional cases of sclerosing hemangioma of the lung in which the cytologic material was prepared at the time of frozen section. Because of the rarity of this lesion and the overlapping cytologic features with other primary malignant lung tumors, awareness of the cytologic features of sclerosing hemangioma may help to avoid a diagnosis of malignancy. This is especially true pre‐ and intraoperatively because a limited resection may suffice if a diagnosis of sclerosing hemangioma can be established. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972033 Sun, 26 Jun 2011 23:00:00 +0100 4972033 http://www.medworm.com/index.php?rid=4972032&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21758 This study tested the efficacy of PAX1 methylation analysis in the triage of cervical ASCUS and ASC‐H and compared its performance with Hybrid Capture 2 (HC2) HPV test. A hospital‐based case–control study was conducted. Cervical scrapings from patients with ASCUS or ASC‐H were used for the quantitative methylation analysis of PAX1 methylation by MethyLight and HPV testing by HC2. Patients with ASC‐H or ASCUS with repeated abnormal smears underwent colposcopic biopsy and subsequent therapies. Diagnoses were made by histopathology at a follow‐up of 2 years. The efficacies of detecting high‐grade lesions were compared. Fifty‐eight cervical scrapings with cytological diagnosis of ASCUS (n = 41) and ASC‐H (n = 17) were analyzed. One of the 41 (2.4%) ASCUS patients and seven of... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972032 Sun, 26 Jun 2011 23:00:00 +0100 4972032 http://www.medworm.com/index.php?rid=4972031&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21769 AbstractUrine cytopathology is a useful and noninvasive tool in the diagnosis and follow‐up of urothelial neoplasia, which remains complementary to emerging molecular tests. These specimens may be challenging and there are numerous mimics and diagnostic pitfalls with which to contend. This review discusses these various entities and includes consideration of ancillary tests that may be useful in the diagnostic procedure. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972031 Sun, 26 Jun 2011 23:00:00 +0100 4972031 http://www.medworm.com/index.php?rid=4972030&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21761 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972030 Sun, 26 Jun 2011 23:00:00 +0100 4972030 http://www.medworm.com/index.php?rid=4972029&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21760 AbstractWe have built a database on the Internet managing z‐axis video for cytology (Zavic), and report on a new style of case discussion supported by the Zavic database. Z‐axis video for cytology is a movie file derived from the video recording of a microscopic field with changes in the focus. We used it for the case presentation of EUS‐FNA of pancreatic lesions on the Internet prior to a training workshop. The attendees were asked to observe the Zavic and to make diagnoses of 20 cases before the workshop. Fourteen attendees also observed lesions under a microscope on that day, and the results were compared with those of Zavic observation. The evaluation of the Zavic database (DB) was surveyed by a questionnaire. The average number of accurate diagnoses by 46 Zavic observers was 10.... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972029 Sun, 26 Jun 2011 23:00:00 +0100 4972029 http://www.medworm.com/index.php?rid=4972028&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21757 AbstractThe CINtec PLUS® system is an immunohistochemical cocktail composed of antibodies against p16INK4a (surrogate of HPV infection) and Ki‐67 (proliferation marker) meant to improve the sensitivity and specificity for detecting high‐grade dysplasia (HGD). In the presence of dysplasia, a red chromogen marks Ki‐67 expression in the nucleus and a brown chromogen marks cytoplasmic p16INK4a expression. Only cells showing dual staining are interpreted as positive. This retrospective study examined the performance of CINtec PLUS testing when performed on ASC‐US diagnosed samples. Comparison was made to high‐risk HPV DNA test results and colposcopic biopsy results. Technical considerations in the interpretation of this immunohistochemical stain are additionally discussed. CINtec PLU... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4972028 Sun, 26 Jun 2011 23:00:00 +0100 4972028 http://www.medworm.com/index.php?rid=4956264&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21756 AbstractHPV detection in fine needle aspirates from suspected head and neck metastasis may be useful in clinching the diagnosis of HPV related oral squamous cell carcinoma. Ascertaining the HPV status of a particular tumor on cytological specimens could be useful for prognostication as HPV‐related tumors appear to have a better prognosis and clinical outcome. The various techniques of detection are reviewed. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4956264 Wed, 22 Jun 2011 22:09:05 +0100 4956264 http://www.medworm.com/index.php?rid=4956268&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21763 AbstractPrimary breast lymphoma (PBL) is a rare disease, which comprises 0.04–0.53% of all primary malignant tumors of the breast. The most frequent histological subtype is diffuse large B‐cell type (DLBCL) (40–70%). Differentiation of PBLs from other breast tumors such as poorly differentiated carcinomas and lobular carcinoma may at times be difficult on cytomorphology alone. An audit of breast lymphomas diagnosed on fine needle aspiration cytology (FNAC) over a period of 9 years (2001–2009) was performed. Ten cases were retrieved and the cytomorphology was reviewed along with immunochemistry (IHC), flow cytometry as well as histopathology, wherever available. The age of patients ranged from 17 to 83 years. Eight cases were diagnosed as non‐Hodgkin's lymphoma, high‐grade on FN... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4956268 Tue, 21 Jun 2011 23:00:00 +0100 4956268 http://www.medworm.com/index.php?rid=4956267&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21762 We report the fine‐needle aspiration (FNA) cytology findings of a case of EAS that involved the left kidney of an 83‐year‐old male. The smears and cell block sections showed pleomorphic epithelioid cells with ample cytoplasm, eccentric nuclei, occasional cytoplasmic hyaline globules, and rare intracytoplasmic lumina. Immunohistochemical stains performed on cellblock sections showed that the tumor cells were positive for vimentin, CD31, CD34, Factor VIII, and CD10, but showed no staining for cytokeratins AE1/AE3 and 8/18, HMB45, CD45, smooth muscle actin, and S100. The morphological and immunohistochemical distinction between EAS, primary tumors of the kidney, and other epithelioid and rhabdoid neoplasms is discussed. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagno... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4956267 Tue, 21 Jun 2011 23:00:00 +0100 4956267 http://www.medworm.com/index.php?rid=4956266&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21765 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4956266 Tue, 21 Jun 2011 23:00:00 +0100 4956266 http://www.medworm.com/index.php?rid=4956265&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21759 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4956265 Tue, 21 Jun 2011 23:00:00 +0100 4956265 http://www.medworm.com/index.php?rid=4940437&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21755 In this study, we searched the diagnostic value of palpable lesions in patients with thoracic pathology suspected clinically and/or radiologically. We prospectively examined the correlations of clinical/radiologic and pathologic findings of 72 palpable lesions from 68 patients who presented with suspect for a thoracic disease from two tertiary medical centers. Thirty‐two lesions (44.4%) were diagnosed as malignant either by fine‐needle aspiration (FNA) only or FNA with confirmatory biopsy. The most common malignancy was non‐small‐cell carcinoma (10) followed by adenocarcinoma (6), and small‐cell carcinoma (5). The most common localization of the palpable lesions was cervical region (20.8%) followed by left supraclavicular (13.8%) and anterior chest wall (13.8%). FNA was effective... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940437 Wed, 15 Jun 2011 23:00:00 +0100 4940437 http://www.medworm.com/index.php?rid=4940436&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21749 We present the cytomorphology of lung adenocarcinomas, including metastases that were diagnosed by cytologic methods and the relationship to both EGFR and KRAS mutational status. We retrospectively reviewed the clinical and cytomorphologic features of 50 lung adenocarcinomas that were tested for both EGFR and KRAS mutations. Cytomorphologic features evaluated included cell size, architectural pattern, nucleoli, intranuclear cytoplasmic inclusions (INCI), mucin, necrosis, squamoid features, lymphocytic response, and histologic features of BAC differentiation. DNA was extracted from a paraffin‐embedded cell block or frozen needle core fragments. Exon 19 deletions and the L858R mutation in exon 21 of EGFR were detected using PCR followed by capillary electrophoresis for fragment sizing. KRA... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940436 Wed, 15 Jun 2011 23:00:00 +0100 4940436 http://www.medworm.com/index.php?rid=4940435&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21730 AbstractPrimary thyroid lymphoma is a very rare disease. Here, we present a case of primary diffuse large B‐cell lymphoma (DLBCL) in a 48‐year‐old female involving thyroid gland. The patient had thyroid swelling for 15 years which rapidly increased during last 5 months. Fine needle aspiration cytology revealed monomorphic large cells arranged discretely. The cells have high nuclear‐cytoplasmic ratio with prominent single to multiple nucleoli. Aggregates of thyroid follicular cells were absent in the smears. A cytodiagnosis of DLBCL was made and a differential diagnosis of lymphocytic thyroiditis was also included. Subsequent histologic examination revealed a high‐grade non‐Hodgkin lymphoma (NHL). Immunohistochemistry showed the tumor cells expressing CD45, CD20, BCl‐6, and tu... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940435 Wed, 15 Jun 2011 23:00:00 +0100 4940435 http://www.medworm.com/index.php?rid=4940434&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21754 AbstractThe Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) has provided a set of uniform diagnostic terminology including benign (B), atypia of undetermined significance (AUS), follicular neoplasm (FN), suspicious for malignancy (SM), malignancy (M), and nondiagnostic (ND) for the interpretation of thyroid fine‐needle aspiration (FNA). We applied this terminology on our 1,382 thyroid aspirates in a community practice setting, which included 539 cases of B (39%), 376 cases of AUS (27.2%), 116 cases of FN (8.4%), 37 cases of malignant (2.7%), 36 cases of SM (2.6%), and 278 cases of ND (20.1%). Two hundred twenty‐one cases (16%) of thyroid FNA had corresponding follow‐up thyroidectomies. Each diagnostic category represented a unique association with risk of malignancy and ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940434 Wed, 15 Jun 2011 23:00:00 +0100 4940434 http://www.medworm.com/index.php?rid=4940433&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21753 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940433 Wed, 15 Jun 2011 23:00:00 +0100 4940433 http://www.medworm.com/index.php?rid=4940432&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21752 The objective of this study is to assess the relationship between protozoa in spontaneously expectorated sputum samples and a range of clinical and immunological variables. Clinical details including age, gender, smoking status, and use of oral or inhaled steroids were recorded for a cohort of 199 patients whose spontaneously expectorated sputum samples were submitted to a Cytology Laboratory in Spain between January 2005 and December 2006. Slides were scanned for protozoa under light microscopy and scanned for monocytes/small macrophages highlighted by immunocytochemistry (CD68 monoclonal antibody). One hundred ninety‐one patients provided adequate sputum samples, of whom 70 had protozoa in their sputum. There was a strong relationship between the presence of protozoa and monocytes/smal... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940432 Wed, 15 Jun 2011 23:00:00 +0100 4940432 http://www.medworm.com/index.php?rid=4940431&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21751 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940431 Wed, 15 Jun 2011 23:00:00 +0100 4940431 http://www.medworm.com/index.php?rid=4916076&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21747 We report a case of isolated cervical leishmanial lymphadenopathy diagnosed by fine‐needle aspiration cytology (FNAC) in apparently cured case of visceral leishmaniasis. A 28‐year‐old female presented with cervical lymphnode enlargement to surgery outpatient department and was subjected for FNAC. Smear showed numerous Leishmania donovani bodies in the cytoplasm of macrophages and giant cells, and extracellular spaces. She was treated by Amphotericin B for alternate 14 days and the size of the lymphnode regressed. She was found asymptomatic for 1 year of follow‐up. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4916076 Thu, 09 Jun 2011 23:00:00 +0100 4916076 http://www.medworm.com/index.php?rid=4906650&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21746 In conclusion, the results demonstrated the inverse expression of slug and E‐cadherin in the majority of malignant pleural fluid cases compared with nonmalignant pleural fluid. The slug protein may be helpful to access the prognosis of patients with pleural fluid. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906650 Mon, 06 Jun 2011 23:00:00 +0100 4906650 http://www.medworm.com/index.php?rid=4906649&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21742 We describe a case in which the cells had undergone squamous metaplasia with nuclear enlargement and increased nuclear to cytoplasmic ratios corresponding to reactive atypia but with fine and evenly distributed chromatin and smooth nuclear contours, which indicated their benign nature. In addition, in this case intracytoplasmic polymorphs and associated extracellular infiltrates of inflammatory cells are noted. The description of this case may help others to consider a cytological diagnosis of prolapsed fallopian tube, thus preventing repeated cauterisations of vault granulation tissue on one hand, and possibly excessive surgical treatment of a mistaken malignant lesion on the other. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906649 Mon, 06 Jun 2011 23:00:00 +0100 4906649 http://www.medworm.com/index.php?rid=4906648&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21735 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906648 Mon, 06 Jun 2011 23:00:00 +0100 4906648 http://www.medworm.com/index.php?rid=4906647&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21727 In this study, we aimed to report a biopsy‐proven case of extrinsic allergic alveolitis (EAA) who presented with nonspecific respiratory symptoms 1 month after having her whole house interior painted. At CT scanning, we observed the ground glass opacities and the micronodular pattern typical for EAA and also a solid, consolidative lung area, highly suggestive of malignancy. The case initially was misinterpreted as a malignant tumor both radiologically and cytologically at CT‐guided transthoracic fine needle aspiration biopsy. The final pathologic diagnosis was given as EAA on frozen section performed during thoracotomy operation. The patient received short‐term steroid treatment and has been doing well for the last 7 months after her operation.As a conclusion, when assessing a cytolo... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906647 Mon, 06 Jun 2011 23:00:00 +0100 4906647 http://www.medworm.com/index.php?rid=4906646&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21724 AbstractMost guidelines currently recommend the testing of human papillomavirus (HPV) in ASCUS cases. The most used method for this purpose is Hybrid Capture II (HCII), but PCR techniques with GP5+/6+ primers can be also applied. Furthermore, the HCII high‐risk probe test for detection of HPV shows cross‐reactivity with low‐risk HPV. Although this cross‐reactivity has been studied in screening populations, it has received little attention in ASCUS cases.To compare the performance of the HCII high‐risk probe test and PCR for the detection of HPV in ASCUS cases.We randomly selected 83 ASCUS cases that were positive for high‐risk HPV by HCII and applied the PCR test using MYO9‐11 and GP5+/6+ primers to samples from these cases.Our results show cross‐reactivity with low‐risk ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906646 Mon, 06 Jun 2011 23:00:00 +0100 4906646 http://www.medworm.com/index.php?rid=4940430&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21750 We present the cytologic and histologic features of a FNA and needle core biopsy, respectively, of an abdominal mass, likely from a gastrointestinal duplication cyst, foregut cyst or a bronchogenic cyst. We also briefly review the differential diagnosis for ciliated cells encountered in abdominal or pelvic FNAs. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4940430 Tue, 31 May 2011 23:00:00 +0100 4940430 http://www.medworm.com/index.php?rid=4925478&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21743 The objective of the present study was designed to investigate whether immunophenotyping affects BAL results and improves diagnostic accuracy.BAL from 61 patients was included in the study. The patients were also submitted to transbronchial biopsy, with a final diagnosis of granulomatous disease [tuberculosis (TB), n = 20; sarcoidosis (SARC), n = 3; and hypersensitivity pneumonitis (HP), n = 4]; idiopathic interstitial pneumonias (IIPs) [idiopathic pulmonary fibrosis (IPF), n = 9; organizing pneumonia (OP), n = 17]; and lung cancer (LC), n = 8. Immunohistochemistry and histomorphometry were used to identify and quantify type 1 and type 2 alveolar epithelial cells, macrophages, CD3+T‐cells, CD4+T‐cells, CD8+T‐cells, and CD20+B‐cells in BAL. These markers were correlated with a datab... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4925478 Tue, 31 May 2011 23:00:00 +0100 4925478 http://www.medworm.com/index.php?rid=4916075&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21745 This study is designed to test the formula “0.06 × (age) + 4.76 × (S/L ratio) + 2.15 × (internal echo) + 1.80 × (vascular pattern)” generated from the study of Liao et al. as a scoring system for predicting LN malignancy in a cytopathologist operated USG‐FNA practice.Eighty‐three reports of USG‐FNA of LNs issued between 7/1/2008 and 4/28/2010 were reviewed. Patient's age, S/L ratio, internal echo, and vascular pattern were used to generate scores based on the aforementioned formula. A score of seven was used as a cutoff for predicting benign (<7) and malignant (>7) LNs. FNA cytology diagnosis, flow cytometric analysis as well as subsequent surgical diagnosis in some cases served as gold standard for statistical analysis.Among 46 USG‐FNA of LNs with scores > 7, 38 w... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4916075 Tue, 31 May 2011 23:00:00 +0100 4916075 http://www.medworm.com/index.php?rid=4906645&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21715 We report here a case of GCT of bone arising from phalanx of finger because of its very unusual location. The tumor was diagnosed on the basis of fine‐needle aspiration cytology and confirmed by histopathology. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4906645 Tue, 31 May 2011 23:00:00 +0100 4906645 http://www.medworm.com/index.php?rid=4879642&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21639 We present FNAC findings in a series of three cases of SST and discuss the cytomorphological features. We have described Call‐Exner like bodies in one of our cases and hence, suggest that these may not be an exclusive feature of granulosa cell tumor. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879642 Tue, 31 May 2011 16:24:05 +0100 4879642 http://www.medworm.com/index.php?rid=4879647&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21741 Conclusions: EBUS‐TBNA is a minimally invasive procedure which provides sufficient sample for definitive primary diagnosis and classification of malignant lymphoma and granulomatous inflammation in patients with mediastinal lymphadenopathy. Rapid on‐site specimen assessment is invaluable for appropriate assignment of sample to ancillary studies. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879647 Mon, 30 May 2011 23:00:00 +0100 4879647 http://www.medworm.com/index.php?rid=4879646&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21737 We report a rare case of Rosai–Dorfman Disease with both nodal and extranodal involvement in a 33‐year‐old woman. The patient had bilateral cervical lymphadenopathy and diffuse thyroid enlargement. Thyroid gland involvement in RDD diagnosed on fine needle aspiration cytology (FNAC) has rarely been reported in literature. FNAC is a useful and reliable tool for the diagnosis of RDD and the biopsy can be avoided in these patients, thus reducing inconvenience to patients. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879646 Mon, 30 May 2011 23:00:00 +0100 4879646 http://www.medworm.com/index.php?rid=4879645&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21732 AbstractElastofibroma is a rare benign, solid, ill‐defined and slow‐growing soft tissue lesion that typically arises between the tip of scapula and chest wall of elderly. Fine needle aspiration (FNA) is a commonly used technique to definitively diagnose the condition. However, the collagenous nature of the mass often results in a paucicellular FNA smear, resulting in a false‐negative report. Herein, we report cytomorphologic, histological, and radiologic findings in five cases. Five cases of elastofibroma were retrospectively retrieved from the cytopathology archives of a teaching hospital. The cytological material was obtained by CT‐guided (n = 2) and ultrasound‐guided (n = 3) FNA. Smears were stained with Diff‐Quik and Papanicolaou stains. Elastic stain and mucicarmine were r... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879645 Mon, 30 May 2011 23:00:00 +0100 4879645 http://www.medworm.com/index.php?rid=4879644&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21729 AbstractAlthough cytological screening for cervical neoplasia has lowered mortality rates, current screening methods are plagued by sub‐optimal sensitivity and/or specificity. The purpose of this study was to compare the performance of the new CellDetect® staining technology as a potential screening tool. This initial, non‐blinded study, utilized samples are taken at a community‐based clinic. The diagnostic results using CellDetect® were compared with the performance of Pap staining and human papilloma virus (HPV) testing on the same material, as well as the follow‐up biopsies. These data were statistically analyzed in terms of sensitivity, specificity, predictive value (N.P.V and P.P.V), and inter‐observer agreement. Bi‐functional CellDetect® staining revealed morphological... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879644 Mon, 30 May 2011 23:00:00 +0100 4879644 http://www.medworm.com/index.php?rid=4879643&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21652 AbstractIntraoperative smear cytology provides a rapid and reliable intraoperative diagnosis and guidance to the neurosurgeon during surgical resection and lesion targeting. It also helps the surgeon to monitor and modify the approach at surgery. The aim of this article was a clinicopathological and cytomorphological intraoperative crush smear correlation in craniopharyngioma. Thirty craniopharyngiomas were included in this study. Twenty‐seven cases were adaCP and only three cases were papCP. This series included 16 (53%) males and 14 (47%) females adult patients, aged from 15 to 86 years (median, 49 year). Two cases were frank errors, 12 cases showed partial correlation, 5 cases showed incomplete typing of the cell type, and 7 cases discrepancy in type of tumors. The percent error was 1... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4879643 Mon, 30 May 2011 23:00:00 +0100 4879643 http://www.medworm.com/index.php?rid=4866939&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21632 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4866939 Fri, 27 May 2011 16:26:09 +0100 4866939 http://www.medworm.com/index.php?rid=4866941&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21707 We present a case of periductal mastitis associated with epithelial hyperplasia, presenting as a subaerolar swelling in a male breast, which was misinterpreted as lobular carcinoma on FNAC. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4866941 Wed, 25 May 2011 23:00:00 +0100 4866941 http://www.medworm.com/index.php?rid=4866940&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21675 We report a case of AGCT presenting as stage IV disease with exfoliation of malignant cells in the pleural and ascitic fluid with exudative effusions. We wish to emphasize on cytomorphologic features of AGCT to differentiate the same from malignant epithelial tumors. A precise diagnosis in such a case may be possible on serous body fluid cytology with a combined clinical and cytopathologic approach. Diagn. Cytopathol. 2011; © 2011 Wiley‐Liss, Inc. (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4866940 Wed, 25 May 2011 23:00:00 +0100 4866940 http://www.medworm.com/index.php?rid=4834799&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21640 (Source: Diagnostic Cytopathology) Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4834799 Thu, 19 May 2011 14:46:18 +0100 4834799 http://www.medworm.com/index.php?rid=4834802&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21733 This study reviewed the cytology cases of SGTTs in the lung and mediastinum diagnosed over a six‐year period at our institution. The specimens included a total of 22 exfoliative or aspiration cytology specimens identified in 16 patients. Two of the cases were primary tumors: adenoid cystic carcinoma (ACC) of the trachea and mucoepidermoid carcinoma (MEC) of the thymus. The remaining 20 tumors were metastases from the parotid, submandibular gland, tongue, nasal cavity, or soft palate. Eight of the 16 patients (50%) had a diagnosis of ACC, four (25%) had salivary duct carcinomas, two (12.5%) had MECs, one (6.25%) was a basaloid tumor, and one (6.25%) was polymorphous low grade adenocarcinoma. In our series, these SGTTs were more commonly metastatic in the lung or mediastinum (87.5% of the ... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4834802 Mon, 16 May 2011 23:00:00 +0100 4834802 http://www.medworm.com/index.php?rid=4834801&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21656 The objective of this study was to evaluate the frequency of the cytological criteria suggestive of HPV infection in the cervical smears of women with a histological diagnosis of CIN. One hundred and sixty‐two women with abnormal cervical smears and a diagnosis of CIN confirmed by histopathology were selected, including 46 cases of CIN 1, 42 of CIN 2 and 74 cases of CIN 3. Koilocytosis was found in 63% of the smears from women with a histopathological diagnosis of CIN 1. This sign was observed in 26.2% and 25.7% of smears of women with a diagnosis of CIN 2 and CIN 3, respectively. Cytomegaly also was frequent in cervical smears of women with histopathological diagnosis of CIN 1 (71.8%). On the other hand, spindle cells and atypical metaplasia were more frequent in women with CIN 2 and CI... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4834801 Mon, 16 May 2011 23:00:00 +0100 4834801 http://www.medworm.com/index.php?rid=4834800&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21646 In this report, we describe a fatal case of toxoplasmosis in a cardiac transplant patient that was diagnosed by respiratory cytopathology. Although the extracellular organisms were well visualized on the Wright‐Giemsa stained cytospin, they were only faintly seen on the Pap‐stained cytospin trapped within mucin and were not easily appreciated on the ThinPrep slides nor the H&E stained cell block sections. An immunohistochemical stain for Toxoplasma performed on the cell block was strongly positive, and an autopsy performed on the patient confirmed disseminated infection. Our case illustrates that the diagnosis of Toxoplasma in exfoliative cytology specimens can be challenging since organisms are not well visualized on ThinPrep or Pap‐stained material; therefore, Wright‐Giemsa s... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4834800 Mon, 16 May 2011 23:00:00 +0100 4834800 http://www.medworm.com/index.php?rid=4823804&cid=s_33622_32_f&fid=33622&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fdc.21723 AbstractPeritoneal mesotheliomas in children are of rare occurrance. We herein report the clinical, radiological, and pathological findings of a rare case of malignant peritoneal mesothelioma occurring in nine‐year‐old female child. The child presented with abdominal distension and awareness of a painless mass in the abdomen which on radiology appeared as a large heterogeneous pelvic mass with peritoneal deposits at multiple sites. To the best of our knowledge, this is the first case of a peritoneal malignant mesothelioma on which fine needle aspiration (FNA) was performed as first line investigation of the primary tumor. The cytological features, major differential diagnoses, and the pitfalls therein are discussed. Histopathology revealed biphasic pattern of mesothelioma which is agai... Diagnostic Cytopathology journals http://www.medworm.com/rss/comments.php?id=4823804 Sun, 15 May 2011 05:59:01 +0100 4823804