MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Epigenetics and Chromatin' source. Thu, 09 Feb 2012 09:43:32 +0100 http://www.medworm.com/index.php?rid=5642469&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F5%2F1%2F4 The integrity of DNA is continuously challenged by metabolism-derived and environmental genotoxic agents that cause a variety of DNA lesions, including base alterations and breaks. DNA damage interferes with vital processes such as transcription and replication, and if not repaired properly, can ultimately lead to premature aging and cancer. Multiple DNA pathways signaling for DNA repair and DNA damage collectively safeguard the integrity of DNA. Chromatin plays a pivotal role in regulating DNA-associated processes, and is itself subject to regulation by the DNA-damage response. Chromatin influences access to DNA, and often serves as a docking or signaling site for repair and signaling proteins. Its structure can be adapted by post-translational histone modifications and nucleosome remodel... Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5642469 Mon, 30 Jan 2012 05:00:00 +0100 5642469 http://www.medworm.com/index.php?rid=5642470&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F5%2F1%2F3 Conclusions: Taken together, these data suggest that G9a is not involved in maintenance of DNA methylation in somatic cells but might play a role in re-initiation of de novo methylation after treatment with hypomethylating drugs, thus serving as a potential target for combinatorial treatments strategies involving DNMTs inhibitors. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5642470 Fri, 27 Jan 2012 05:00:00 +0100 5642470 http://www.medworm.com/index.php?rid=5590967&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F5%2F1%2F2 Conclusions: Taken together, the data indicate that MEN1 contributes to STAT1 activated gene expression in a novel manner that includes defining the TSS and RNA processing. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5590967 Thu, 12 Jan 2012 05:00:00 +0100 5590967 http://www.medworm.com/index.php?rid=5575575&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F5%2F1%2F1 Regulatory DNA elements like enhancers, silencers, and insulators are embedded in metazoan genomes, and they control gene expression during development. Although they fulfil different roles, they share specific properties. Some examples are discussed, and a parsimonious model for their function proposed; all are transcription units that tether their target promoters close to, or distant from, transcriptional hot-spots (or 'factories'). (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5575575 Mon, 09 Jan 2012 05:00:00 +0100 5575575 http://www.medworm.com/index.php?rid=5415664&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F21 Conclusions: By exploring the spatial organization of the Kcnq1 locus, our results reveal a novel mechanism by which local activation of genes can override the regional silencing effects of non-coding RNAs. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5415664 Tue, 15 Nov 2011 05:00:00 +0100 5415664 http://www.medworm.com/index.php?rid=5394555&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F20 Conclusions: Taken together our data indicate that Miz-1 and Myc maintain human ES cell pluripotency by coordinately suppressing differentiation genes, particularly Hox genes. These data also support a new model of how Myc and Miz-1 function on chromatin. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5394555 Fri, 04 Nov 2011 04:00:00 +0100 5394555 http://www.medworm.com/index.php?rid=5353760&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F19 Conclusions: We describe a novel proteome-wide methodology for the identification of new PKMTs substrates. This technological advance may lead to a better understanding of the enzymatic activity and substrate specificity of the more than 50 PKMTs present in the human proteome, most of which are uncharacterized. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5353760 Mon, 24 Oct 2011 04:00:00 +0100 5353760 http://www.medworm.com/index.php?rid=5320186&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F18 Conclusions: Our data indicate that the intracellular trafficking of SMCX is controlled by its association with PCNA. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5320186 Thu, 13 Oct 2011 04:00:00 +0100 5320186 http://www.medworm.com/index.php?rid=5300919&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F17 Conclusion: We conclude that Tsix and pluripotency factors act synergistically to repress Xist in undifferentiated ES cells. Double mutants do not exhibit maximal levels of Xist expression, indicating that other pathways also play a role. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5300919 Fri, 07 Oct 2011 04:00:00 +0100 5300919 http://www.medworm.com/index.php?rid=5202306&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F16 Conclusions: Our data reveal another important layer of epigenetic control orchestrating skeletal muscle cell terminal differentiation, and introduce a novel function of the PRC2-Ezh1 complex in promoter setting. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5202306 Mon, 05 Sep 2011 04:00:00 +0100 5202306 http://www.medworm.com/index.php?rid=5101797&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F15 ${item.shortDescription} (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5101797 Thu, 04 Aug 2011 23:00:00 +0100 5101797 http://www.medworm.com/index.php?rid=5046624&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F11 ${item.shortDescription} (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5046624 Tue, 19 Jul 2011 23:00:00 +0100 5046624 http://www.medworm.com/index.php?rid=5025784&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F10 In this study, we take advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs. Results: By comparing the promoter methylation status of over 14,000 genes in human placentas from 10 diandries (extra paternal haploid set) and 10 digynies (extra maternal haploid set), using 6 complete hydatidiform moles (paternal origin) and 10 chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=5025784 Tue, 12 Jul 2011 23:00:00 +0100 5025784 http://www.medworm.com/index.php?rid=4900180&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F9 Conclusions: While truly "poised" bivalently modified genes may exist, the original hypothesis that all bivalent genes are epigenetically pre-marked for subsequent expression might be over-simplistic. In fact from the data presented here, it is equally possible that many genes, which appear to be bivalent in pluripotent and multipotent cells, may simply be stochastically expressed at low levels in the process of multi-lineage priming. Although both situations could be considered to be forms of "poising", the underlying mechanisms and the associated implications are clearly different. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4900180 Sun, 05 Jun 2011 23:00:00 +0100 4900180 http://www.medworm.com/index.php?rid=4797315&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F8 After publication of this work [Vinayachandran et al., 2009], we noticed that there was an inadvertent oversight due to which Figure 5 and 6 Legends were interchanged. While the figures and their captions are correct, the legends should have been interchanged and read as follows. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4797315 Fri, 06 May 2011 23:00:00 +0100 4797315 http://www.medworm.com/index.php?rid=4787165&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F7 Conclusions: Most fetal tDMRs seem to reflect transient DNA methylation changesduring development rather than permanent epigenetic signatures. The extensive tissuespecificand developmental-stage specific nature of DNA methylation will need to be3elucidated to identify abnormal patterns of DNA methylation associated with abnormaldevelopment or disease. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4787165 Wed, 04 May 2011 23:00:00 +0100 4787165 http://www.medworm.com/index.php?rid=4702530&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F6 Conclusions: This study provides insights into the role that MLE plays in the function of the MSL complex through its association with roX RNAs and the other MSL subunits and suggests a hypothesis to explain the role of MLE in the synthesis of these RNAs. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4702530 Mon, 11 Apr 2011 23:00:00 +0100 4702530 http://www.medworm.com/index.php?rid=4609584&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F5 Conclusion: Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4609584 Fri, 18 Mar 2011 00:00:00 +0100 4609584 http://www.medworm.com/index.php?rid=4599317&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F4 Conclusions: This analysis showed that different PREs have quantifiably different properties, and that changes in as few as four base pairs have profound effects on PRE function, thus illustrating the power and sensitivity of C31 site-specific integration as a tool for the rapid and quantitative dissection of elements of PRE design. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4599317 Wed, 16 Mar 2011 00:00:00 +0100 4599317 http://www.medworm.com/index.php?rid=4520957&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F3 Conclusions: : These data support a model in which general chromatin factors independent of both DNA sequence and CENH3 enforce roughly uniform centromeric nucleosome spacing while allowing flexibility in the mode in which nucleosomes are positioned. In the case of tandem repeat DNA, the natural bending effects related to AA/TT periodicity produce an energetically-favorable arrangement consistent with conformationally rigid nucleosomes and stable chromatin at centromeres. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4520957 Fri, 25 Feb 2011 00:00:00 +0100 4520957 http://www.medworm.com/index.php?rid=4432162&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F2 Conclusions: Targeting of Dot1 promoted gene expression by antagonizing gene repression through both histone methylation and chromatin relocalization. Our findings show that binding of Dot1 to chromatin can positively affect local gene expression by chromatin rearrangements over a considerable distance. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4432162 Thu, 03 Feb 2011 00:00:00 +0100 4432162 http://www.medworm.com/index.php?rid=4417982&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F4%2F1%2F1 Conclusions: Our validated pyrosequencing methylation assays can be widely used as a tool to investigate DNA methylation levels of imprinted genes in clinical samples. This first comprehensive analysis of normal methylation levels in adult somatic tissues at human imprinted regions confirm that, despite intra-individual variability and tissue specific expression, imprinted genes faithfully maintain their DNA methylation in healthy adult tissue. DNA methylation levels of a selection of imprinted genes are, therefore, a valuable indicator for epigenetic stability. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4417982 Mon, 31 Jan 2011 00:00:00 +0100 4417982 http://www.medworm.com/index.php?rid=4211882&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F21 Conclusions: These studies demonstrate a cell-autonomous role for Brg1 in lens fiber cell terminal differentiation and identified DNase IIbeta as a potential direct target of SWI/SNF complexes. Brg1 is directly or indirectly involved in processes that degrade lens fiber cell chromatin. The presence of nuclei and other organelles generates scattered light incompatible with the optical requirements for the lens. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4211882 Tue, 30 Nov 2010 00:00:00 +0100 4211882 http://www.medworm.com/index.php?rid=4157684&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F20 Conclusion: Our findings may be combined into a hypothesis for the emergence of a weak nucleosome-positioning code. According to this hypothesis, consistent nucleosomes may be partly guided by nearby nucleosome-free regions through statistical positioning. Once established, a set of well-positioned consistent nucleosomes may impose secondary constraints which further shape the structure of the underlying DNA. We are able to capture these constraints through the application of a recently introduced structural property that is related to the symmetry of DNA curvature. Furthermore we show that both consistently-positioned nucleosomes and their adjacent nucleosome-free regions show an increased tendency for the conservation of this structural feature. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4157684 Fri, 12 Nov 2010 00:00:00 +0100 4157684 http://www.medworm.com/index.php?rid=4148751&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F19 Conclusions: UBF is the first common interaction partner of CTCF and CTCFL, suggesting a role for these proteins in chromatin organization of the rDNA repeats. We propose that CTCF affects RNA polymerase I-mediated events globally by controlling nucleolar number, and locally by regulating chromatin at the rDNA spacer promoter, similar to RNA polymerase II promoters. CTCF may load UBF onto rDNA, thereby forming part of a network that maintains rDNA genes poised for transcription. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4148751 Mon, 08 Nov 2010 00:00:00 +0100 4148751 http://www.medworm.com/index.php?rid=4084899&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F18 Histone acetylation is one of the key regulatory mechanisms controlling transcriptional activity in eukaryotic cells. In higher eukaryotes, a number of nuclear histone acetyl-transferase (HAT) enzymes have been identified, most of which are part of a large multi-subunit complex. This diversity, combined with the large number of potentially acetylable lysines on histones suggested the existence of a specific regulatory mechanism based on the substrate specificity of HATs. Over the last decade, intensive characterisations of the HAT complexes have been carried out. However, the precise mode of action of HATs and particularly the functional differences amongst these complexes remain elusive. Here we review current insights into the functional role of HATs focusing on the specificity of their ... Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=4084899 Tue, 19 Oct 2010 23:00:00 +0100 4084899 http://www.medworm.com/index.php?rid=3997051&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F17 Conclusions: These data indicate that DNA methylation in pluripotent stem cells is much more dynamic and error-prone than is maintenance methylation in differentiated cells. DNA methylation requires DNMT3L in stem cells but DNMT3L is not expressed in differentiating somatic cells. Error-prone maintenance methylation will introduce unpredictable phenotypic variation into clonal populations of pluripotent stem cells, and this variation is likely to be much more pronounced in cultured female cells. This epigenetic variability has obvious negative implications for the clinical applications of stem cells. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3997051 Thu, 23 Sep 2010 23:00:00 +0100 3997051 http://www.medworm.com/index.php?rid=3945096&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F16 Conclusions: H2B monoubiquitination promotes H3K4 methylation, but the E3 ubiquitin ligase, RNF20, is repressive of inducible transcription at the IRF1 gene locus, suggesting that ubH2B can, directly or indirectly, affect Pol II CTD phosphorylation cycling to exert control on ongoing transcription. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3945096 Tue, 07 Sep 2010 23:00:00 +0100 3945096 http://www.medworm.com/index.php?rid=3860934&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F15 Conclusions: Our data suggest a model in which MES-4 helps to maintain an "epigenetic memory" of transcription that occurred in germ cells of previous generations, and that MES-4 and its epigenetic product are essential for normal germ cell development. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3860934 Wed, 11 Aug 2010 23:00:00 +0100 3860934 http://www.medworm.com/index.php?rid=3801391&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F14 Conclusions: We have characterized both mH2A subtypes from carp fish, and evaluated their participation in the regulation of the ribosomal cistron. Our findings revealed that the differential incorporation of mH2A subtypes could regulate gene expression during the acclimatization process of carp. In this context, we report new evidence that reveal a differential chromatin incorporation of mH2A subtypes through the environmental adaptation process, which concurs with antagonist transcriptional states in the carp ribosomal cistron. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3801391 Wed, 28 Jul 2010 23:00:00 +0100 3801391 http://www.medworm.com/index.php?rid=3732341&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com Conclusions: These results demonstrate an ability of DNA sequences selected solely for nucleosome affinity to organize chromatin in vivo, and the ability of other mechanisms to overcome these interactions in a dynamic nuclear environment. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3732341 Wed, 30 Jun 2010 23:00:00 +0100 3732341 http://www.medworm.com/index.php?rid=3541363&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F10 Conclusions: This study provides insight into the requirements for efficient Xist mediated silencing and specifically identifies organisation of the chromosome into gene-rich L1-depleted and gene-poor L1-dense domains as a major influence on the ability of Xist-mediated silencing to be propagated in a continuous manner in cis. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3541363 Thu, 06 May 2010 23:00:00 +0100 3541363 http://www.medworm.com/index.php?rid=3507163&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F9 Conclusions: The Cbx3 gene product (the HP1gamma protein) has a non-redundant function during spermatogenesis that cannot be compensated for by the other two HP1 isotypes. The Cbx3hypo/hypo spermatogenesis defect is similar to that found in Miwi2 and Dnmt3L mutants. The Cbx3 gene-targeted mice generated in this study provide an appropriate model for the study of HP1gamma in transposon silencing and parental imprinting. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3507163 Mon, 26 Apr 2010 23:00:00 +0100 3507163 http://www.medworm.com/index.php?rid=3427878&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F8 Conclusions: Because TBG is the main carrier of the thyroid hormone T4, which regulates energy metabolism, we propose that overexpression of TBG is responsible for the fat accumulation observed in H2afy-deficient liver. Moreover, our results suggest that the sexual dimorphism of the steatotic phenotype is probably due to the different incorporation of macroH2A1 in males and females. In combination with previous studies, our data demonstrate a role for macroH2A1 in regulating homeostasis in a sex-dependent manner, subject to genetic background. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3427878 Wed, 31 Mar 2010 23:00:00 +0100 3427878 http://www.medworm.com/index.php?rid=3397890&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F7 Conclusions: We have characterised a novel methylation site in the C-terminus of H1 that is the target of G9a/Glp1 both in vitro and in vivo. To our knowledge, this is the first demonstration of variant-specific histone methylation by the same methyltransferases, but with differing downstream readers, thereby supporting the hypothesis of H1 variants having specific functions. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3397890 Wed, 24 Mar 2010 00:00:00 +0100 3397890 http://www.medworm.com/index.php?rid=3238575&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F5 Conclusion: Taken together, these experiments suggest that the 19S proteasome plays a crucial role in the initial reorganization of events enabling the relaxation of the repressive chromatin structure surrounding inducible promoters. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3238575 Thu, 04 Feb 2010 00:00:00 +0100 3238575 http://www.medworm.com/index.php?rid=3184614&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F3 Conclusions: Our study suggests that epigenetic transgene silencing can result from convergent transcription of inverted repeats which can lead to silencing of an entire multi-copy transgene array. This mechanism may account for a significant proportion of the reported cases of transgene inactivation in mice. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3184614 Tue, 19 Jan 2010 00:00:00 +0100 3184614 http://www.medworm.com/index.php?rid=3178086&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F2 Conclusion: A large amount of data is produced from whole-genome expression and epigenetic profiling studies of cellular material. We have shown that such publicly available data can be mined and analysed in order to generate novel findings for categories of genes or regulatory elements. Comparing two types of gene regulation, imprinting and developmental, our results suggest that different histone modifications associate with these distinct processes. This form of analysis is therefore a useful tool to elucidate the complex epigenetic code associated with genome function and to determine the underlying features conferring epigenetic states. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3178086 Fri, 15 Jan 2010 00:00:00 +0100 3178086 http://www.medworm.com/index.php?rid=3162832&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F3%2F1%2F1 Conclusions: These data provide evidence that the diversification of defined embryonic and extra-embryonic lineages is accompanied by chromatin remodelling at specific loci. Stem cell lines from the ICM, TE and PrE can each dominantly reprogramme somatic cells but reset gene expression differently, reflecting their separate lineage identities and increasingly restricted developmental potentials. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3162832 Tue, 12 Jan 2010 00:00:00 +0100 3162832 http://www.medworm.com/index.php?rid=3047187&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F16 Conclusions: PcG silencers bind and coordinately regulate INK4b and INK4a, but not ARF, during a variety of physiological processes. Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. Our results revealed a chromatin looping mechanism of long-range control and argue against models involving homogeneous spreading of PcG silencers across the INK4b-ARF-INK4a locus. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3047187 Wed, 02 Dec 2009 00:00:00 +0100 3047187 http://www.medworm.com/index.php?rid=3047186&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F17 Conclusions: Our results suggest a central role of upstream promoter acetylation in the quantitative regulation of gene expression in this model gene. Induced core promoter acetylation is dispensable for the highest gene expression in the diurnal and circadian rhythm. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3047186 Wed, 02 Dec 2009 00:00:00 +0100 3047186 http://www.medworm.com/index.php?rid=3047185&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F18 Conclusions: Our data identifies chromatin features that correlate with the silencing state and indicate that an array of phased nucleosomes is not sufficient for full repression. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=3047185 Wed, 02 Dec 2009 00:00:00 +0100 3047185 http://www.medworm.com/index.php?rid=2995029&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F15 Conclusions: Our experiments show a genetic and biochemical interaction between RNA Pol II (largest and second largest subunits) and the small RNA silencing machinery in Drosophila. The interaction has functional aspects in terms of determining H3K9me2 and HP-1 deposition at the chromocentric heterochromatin. Thus, RNA Pol II has an important role in establishing heterochromatin structure in Drosophila. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2995029 Mon, 16 Nov 2009 00:00:00 +0100 2995029 http://www.medworm.com/index.php?rid=2950040&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F13 Epigenetics & Chromatin published its first papers a year ago, together with an editorial in which we stated our aim to publish a high-quality journal with a broad scope. A year later we are happy to report that these aims are being achieved. Papers published so far represent a broad swath of research on chromatin-based processes and epigenetic mechanisms. We are also delighted with the quality and breadth of submissions from many of the leading laboratories in the field. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2950040 Mon, 02 Nov 2009 00:00:00 +0100 2950040 http://www.medworm.com/index.php?rid=2868097&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F12 Conclusions: Loss of ESET or Oct4 results in strikingly similar phenotypes both in ES cells with their differentiation into trophectoderm cells, and in early embryos where there is a failure of development of the pluripotent inner cell mass (ICM) of blastocysts. We propose that SUMOylated ESET-Oct4 complex is critical for both the initiation and maintenance of pluripotency through repression of differentiation, particularly of the trophectoderm lineage by epigenetic silencing of Cdx2. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2868097 Tue, 06 Oct 2009 23:00:00 +0100 2868097 http://www.medworm.com/index.php?rid=2784947&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F11 Conclusions: Our results suggest that there may be a memory or an epigenetic mark on the nucleosome-depleted PHO5 promoter that is independent of the transcription apparatus and maintains the promoter in a nucleosome-depleted state through DNA replication. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2784947 Thu, 10 Sep 2009 23:00:00 +0100 2784947 http://www.medworm.com/index.php?rid=2616409&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F8 Conclusions: These data point toward a role for transcription of non-coding RNAs as a developmental strategy for the establishment of functionally distinct domains within the mammalian genome. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2616409 Sun, 19 Jul 2009 23:00:00 +0100 2616409 http://www.medworm.com/index.php?rid=2467172&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F7 Conclusion: The varied patterns of methylation differences detected between tissues by our methylation profiling method reinforce the potential functional significance of regional differences in methylation levels outside of CpG islands. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2467172 Mon, 08 Jun 2009 04:00:00 +0100 2467172 http://www.medworm.com/index.php?rid=2411620&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F6 Conclusions: In agreement with its proposed role in the initiation of DNA replication, GINS proteins associated with chromatin near sites of ORC binding that were devoid of EdU (absence of DNA replication). The association of GINS proteins with PCNA was consistent with a role in the process of elongation. Additionally, the large size of our chromatin fibers (up to approximately 7 Mb) allowed for a more expansive analysis of the distance between active replicons than previously reported. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2411620 Thu, 14 May 2009 04:00:00 +0100 2411620 http://www.medworm.com/index.php?rid=2320539&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F5 Conclusions: These data suggest a bimodal recruit and maintain model whereby Mll2 is required to establish certain epigenetic decisions during differentiation, which are then maintained by redundant mechanisms. We also suggest that these mechanisms relate to the epigenetic maintenance of CpG island promoters. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2320539 Mon, 06 Apr 2009 04:00:00 +0100 2320539 http://www.medworm.com/index.php?rid=2272037&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F4 Conclusions: On a gene locus, multiple nucleosome positions are directed by a gene sequence to provide a pool of possibilities, out of which the preferred ones are selected by the chromatin remodeler and transcription factor of the gene under different states of activity of the gene. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2272037 Mon, 16 Mar 2009 04:00:00 +0100 2272037 http://www.medworm.com/index.php?rid=2255542&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F3 Conclusions: In this study we demonstrate the validity of using pooled DNA samples to accurately assess group DNA methylation averages. Such an approach can be readily applied to the assessment of disease phenotypes reducing the time, cost and amount of DNA starting material required for large-scale epigenetic analyses. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2255542 Tue, 10 Mar 2009 04:00:00 +0100 2255542 http://www.medworm.com/index.php?rid=2196396&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F2 Spinocerebellar ataxia (SCA) is a physically devastating, genetically inherited disorder characterized by abnormal brain function that results in the progressive loss of the ability to coordinate movements. There are many types of SCAs as there are various gene mutations that can cause this disease. SCA types 1-3, 6-10, 12, and 17 result from a trinucleotide repeat expansion in the DNA-coding sequence. Intriguingly, recent work has demonstrated that increased trinucleotide repeat expansions result in defects in the function of the SAGA histone acetyltransferase complex. The SCA7 gene encodes a subunit of the SAGA complex. This subunit is conserved in yeast as the SGF73 gene. We demonstrate that SGF73 is required to recruit the histone deubiquitination module into both SAGA and the related ... Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2196396 Wed, 18 Feb 2009 05:00:00 +0100 2196396 http://www.medworm.com/index.php?rid=2142604&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F2%2F1%2F1 Conclusions: Our molecular maps demonstrate that heterochromatin can invade a normally euchromatic region, yet the strength of HP1 binding and effects on gene expression are highly dependent on local context. Our data suggest that the white gene has an unusual intrinsic affinity for heterochromatin, which may cause this gene to be more sensitive to PEV than most other genes. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2142604 Thu, 29 Jan 2009 05:00:00 +0100 2142604 http://www.medworm.com/index.php?rid=2043757&cid=s_38184_50_f&fid=38184&url=http%3A%2F%2Fwww.epigeneticsandchromatin.com%2Fcontent%2F1%2F1%2F10 Conclusions: Efficient and highly specific recognition of CenH3 in histone core particles isolated from native centromeric chromatin demonstrates that tetramers are the predominant form of centromeric nucleosomes in mature tetramers. Our findings provide proof of principle that this approach can yield insights into chromatin biology using direct and rapid detection of native nucleosomes in physiological salt concentrations. (Source: Epigenetics and Chromatin) Epigenetics and Chromatin journals http://www.medworm.com/rss/comments.php?id=2043757 Wed, 17 Dec 2008 05:00:00 +0100 2043757