MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Experimental Hematology' source. Wed, 08 Feb 2012 17:42:01 +0100 http://www.medworm.com/index.php?rid=5583485&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005996%2Fabstract%3Frss%3Dyes See Ran et al., pages 155–165. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583485 Mon, 26 Dec 2011 05:00:00 +0100 5583485 http://www.medworm.com/index.php?rid=5583493&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005467%2Fabstract%3Frss%3Dyes Hematopoietic stem cells (HSCs) are maintained at a very low frequency in adult bone marrow under steady-state conditions. However, it is not fully understood how homeostasis of bone marrow HSCs is maintained. We attempted to identify a key molecule involved in the regulation of HSC numbers, a factor that, in the absence of Lnk, leads to HSC expansion. Here, we demonstrate that upon stimulation with thrombopoietin, expression of Bcl-xL, an antiapoptotic protein, was highly enhanced in Lnk-deficient HSCs compared to normal HSCs. As a result, Lnk-deficient HSCs underwent reduced apoptosis following exposure to lethal radiation. Downregulation of Bcl-xL expression in Lnk-deficient HSCs by short-hairpin RNA resulted in a great reduction of their capacity for reconstitution. These findings sugg... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583493 Mon, 21 Nov 2011 05:00:00 +0100 5583493 http://www.medworm.com/index.php?rid=5583486&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005455%2Fabstract%3Frss%3Dyes Thrombocytopenia (TCP) is a dose-limiting toxicity of histone deacetylase inhibitors in both single-agent and combination therapy strategies. A recent report in Blood by Bishton et al. demonstrated preclinical evidence that TCP induced by histone deacetylase inhibitors can be mitigated by concurrent use of a thrombopoietin (TPO) mimetic agent. Based on our earlier work showing rapid recovery from TCP induced by the proteasome inhibitor bortezomib , and promising preclinical and clinical efficacy data for bortezimib + romidepsin combination therapy , we conducted studies in mice to determine whether combination bortezomib + romidepsin induces greater severity of TCP compared to each drug alone, examine platelet recovery kinetics, and test effects on bone marrow megakaryocytes. (Source: Exp... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583486 Mon, 21 Nov 2011 05:00:00 +0100 5583486 http://www.medworm.com/index.php?rid=5583489&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100542X%2Fabstract%3Frss%3Dyes We describe and apply newly developed computational algorithms that allow for an automated generation of high-content data of single-cell characteristics at high temporal and spatial resolution, together with the reconstruction and statistical evaluation of complete genealogical histories. This methodology has been applied to the particular example of purified primary human HSPCs in bioengineered culture conditions. The combination of genealogical information and dynamic profiles of cellular properties identified a marked symmetry between sibling HSPCs regarding cell cycle time, but also migration speed and growth kinetics. Furthermore, we demonstrate that this symmetry of HSPC siblings can be altered by exogenous cues of the local biomimetic microenvironment. Using the example of HSPC gro... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583489 Tue, 15 Nov 2011 05:00:00 +0100 5583489 http://www.medworm.com/index.php?rid=5583487&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005431%2Fabstract%3Frss%3Dyes In order to develop minimally toxic bone marrow transplantation (BMT) protocols suitable for use in a wider range of indications, it is important to identify ways to enhance BM engraftment at a given level of recipient conditioning. CXCL12/stromal cell-derived factor-1α plays a crucial physiological role in homing of hematopoietic stem cells to BM. It is regulated by the ectopeptidase dipeptidyl peptidase IV (DPPIV; DPP4) known as CD26, which cleaves dipeptides from the N-terminus of polypeptide chains. Blocking DPPIV enzymatic activity had a beneficial effect on hematopoietic stem cell engraftment in various but very specific experimental settings. Here we investigated whether inhibition of DPPIV enzymatic activity through Diprotin A or sitagliptin (Januvia) improves BM engraftment in no... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583487 Tue, 15 Nov 2011 05:00:00 +0100 5583487 http://www.medworm.com/index.php?rid=5583491&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005054%2Fabstract%3Frss%3Dyes Novel roles for the serine protease plasmin have been implicated recently in physiological and pathological processes. However, whether plasmin is involved in erythropoiesis is not known. In the present study, we studied the consequences of plasminogen deficiency on erythropoiesis in plasminogen-deficient (Plg knockout [KO]) mice. Erythroid differentiation was attenuated in male Plg KO mice and resulted in erythroblastic accumulation within the spleen and bone marrow, with increased apoptosis in the former, erythrocytosis, and splenomegaly, whereas similar erythropoietic defect was less prominent in female Plg KO mice. In addition, erythrocyte lifespan was shorter in both male and female Plg KO mice. Erythropoietin levels were compensatory increased in both male and female Plg KO mice, and... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583491 Fri, 04 Nov 2011 04:00:00 +0100 5583491 http://www.medworm.com/index.php?rid=5583492&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005029%2Fabstract%3Frss%3Dyes Leukemia stem cell candidates (LSCC) can be enriched from patients with acute myeloid leukemia by high aldehyde dehydrogenase (ALDH) activity and CD34 expression. We have previously demonstrated the leukemia-initiating activity of ALDHbright cells in xenograft transplantation models, as well as in vitro. Applying single-cell long-term culture-initiating cell assays, we have correlated the functional properties of individual cells within this LSCC population and the respective phenotypes. To define their biologic significance, we also analyzed the relationship between LSCC at diagnosis to long-term clinical outcomes. The median percentage of ALDHbright cells among 101 acute myeloid leukemia patients was 0.51% (range, 0.01–12.90%). Single-cell long-term culture-initiating cell assays, fol... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583492 Mon, 24 Oct 2011 04:00:00 +0100 5583492 http://www.medworm.com/index.php?rid=5583490&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005030%2Fabstract%3Frss%3Dyes This study aims to examine the effect of p53 loss on in vivo megakaryopoiesis, platelet production, and function, and to investigate the basis for greater ploidy in p53−/− megakaryocytic cultures. Here, we used flow cytometry to analyze ploidy, DNA synthesis, and apoptosis in murine cultured and bone marrow megakaryocytes following thrombopoietin administration and to analyze fibrinogen binding to platelets in vitro. Culture of p53−/− marrow cells for 6 days with thrombopoietin gave rise to 1.7-fold more megakaryocytes, 26.1% ± 3.6% of which reached ploidy classes ≥64 N compared to 8.2% ± 0.9% of p53+/+ megakaryocytes. This was due to 30% greater DNA synthesis in p53−/− megakaryocytes and 31% greater apoptosis in p53+/+ megakaryocytes by day 4 of culture. Although the bon... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583490 Mon, 24 Oct 2011 04:00:00 +0100 5583490 http://www.medworm.com/index.php?rid=5583488&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005017%2Fabstract%3Frss%3Dyes This article is the first to show that stem-like MCL cells utilize constitutive NF-κB expression for survival. Given that the NF-κB expression in MCL-ICs is resistant to bortezomib, it will be important to find alternative therapeutic strategies to inhibit NF-κB expression. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5583488 Mon, 24 Oct 2011 04:00:00 +0100 5583488 http://www.medworm.com/index.php?rid=5487932&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100498X%2Fabstract%3Frss%3Dyes The ability to expand hematopoietic stem and progenitor cells (HSPCs) in vitro will enhance the success of a wide range of transplant-related therapies. PTEN (phosphatase and tensin homologue deleted on chromosome 10) has been implicated as a regulator of murine HSPC self-renewal, but little is understood about the role of PTEN in human HSPC regulation. We tested the impact of transient small interfering RNA (siRNA)–induced inhibition of PTEN expression in human CD34+ cells on their cell cycle profile, their susceptibility to retroviral transduction, and their ability to self-renew and repopulate nonobese diabetic/severe combined immunodeficiency disease with interleukin-2 receptor γ-chain deficiency mice. Reduced PTEN messenger RNA and protein levels were confirmed in PTEN siRNA-treat... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487932 Mon, 24 Oct 2011 04:00:00 +0100 5487932 http://www.medworm.com/index.php?rid=5487928&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004991%2Fabstract%3Frss%3Dyes In conclusion, we found that hyperchemosensitivity of normal nonleukemic CFU-GM is associated with a high risk of CD34+ cell mobilization failure, while a chemoresistant pattern in CFU-GM and BFU-E is associated with poor disease-free survival and increased cumulative incidence of relapse. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487928 Mon, 24 Oct 2011 04:00:00 +0100 5487928 http://www.medworm.com/index.php?rid=5487927&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005005%2Fabstract%3Frss%3Dyes Hyperkinetic Jak2 tyrosine kinase signaling has been implicated in several hematological disorders, including myeloproliferative neoplasms. Effective Jak2 inhibitors can have significant therapeutic potential. Here, using structure-based virtual screening, we identified a benzothiophene-derived Jak2 inhibitor named A46. We hypothesized that this compound would inhibit Jak2-V617F–mediated pathologic cell growth. To test this, A46 was analyzed for its ability to inhibit recombinant Jak2 protein catalysis; suppress Jak2-mediated pathogenic cell growth in vitro; inhibit the aberrant ex vivo growth of Jak2-V617F–expressing primary human bone marrow cells; and inhibit Jak2-mediated pathogenesis in vivo. To this end, we found that A46 selectively inhibited Jak2-V617F protein when compared ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487927 Mon, 24 Oct 2011 04:00:00 +0100 5487927 http://www.medworm.com/index.php?rid=5409658&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11005042%2Fabstract%3Frss%3Dyes See Porada et al., pages 1124–1135. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409658 Mon, 24 Oct 2011 04:00:00 +0100 5409658 http://www.medworm.com/index.php?rid=5487925&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004905%2Fabstract%3Frss%3Dyes Purine analogs such as 6-thioguanine (6TG) cause myelotoxicity upon conversion into nucleotides by hypoxanthine-guanine phosphoribosyltransferase (HPRT). Here we have developed a novel and highly efficient strategy employing 6TG as a single agent for both conditioning and in vivo chemoselection of HPRT-deficient hematopoietic stem cells. The dose-response and time course of 6TG myelotoxicity were first compared in HPRT wild-type mice and HPRT-deficient transgenic mice. Dosage and schedule parameters were optimized to employ 6TG for myelosuppressive conditioning, immediately followed by in vivo chemoselection of HPRT-deficient transgenic donor bone marrow (BM) transplanted into syngeneic HPRT wild-type recipients. At appropriate doses, 6TG induced selective myelotoxicity without any adver... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487925 Thu, 13 Oct 2011 04:00:00 +0100 5487925 http://www.medworm.com/index.php?rid=5324646&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004577%2Fabstract%3Frss%3Dyes See Trummer et al., pages 1047–1055. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324646 Mon, 26 Sep 2011 04:00:00 +0100 5324646 http://www.medworm.com/index.php?rid=5487931&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004553%2Fabstract%3Frss%3Dyes Transient receptor potential (TRP) channels Trpc2 and Trpc3 are expressed on normal murine erythroid precursors, and erythropoietin stimulates an increase in intracellular calcium ([Ca2+]i) through TRPC2 and TRPC3. Because modulation of [Ca2+]i is an important signaling pathway in erythroid proliferation and differentiation, Trpc2, Trpc3, and Trpc2/Trpc3 double knockout mice were utilized to explore the roles of these channels in erythropoiesis. Trpc2, Trpc3, and Trpc2/Trpc3 double knockout mice were not anemic, and had similar red blood cell counts, hemoglobins, and reticulocyte counts as wild-type littermate controls. Although the erythropoietin-induced increase in [Ca2+]i was reduced, these knockout mice showed no defects in red cell production. The major phenotypic difference at steady... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487931 Thu, 15 Sep 2011 04:00:00 +0100 5487931 http://www.medworm.com/index.php?rid=5487929&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004565%2Fabstract%3Frss%3Dyes In conclusion, our group has developed a sensitized random mutagenesis screen utilizing 5FU and has shown that the strain combination of 129/SvImJ × C57BL/6J is robust for identification of founder lines with defects in megakaryocyte and platelet development. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487929 Thu, 15 Sep 2011 04:00:00 +0100 5487929 http://www.medworm.com/index.php?rid=5487930&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004528%2Fabstract%3Frss%3Dyes The goal of this study was to determine if we could establish a mesenchymal stromal line from zebrafish that would support hematopoietic cells. Such a coculture system would be a great benefit to study of the hematopoietic cell−stromal cell interaction in both in vitro and in vivo environments. Zebrafish stromal cells (ZStrC) were isolated from the “mesenchymal” tissue of the caudal tail and expanded in a specialized growth media. ZStrC were evaluated for phenotype, gene expression, and ability to maintain zebrafish marrow cells in coculture experiments. ZStrC showed mesenchymal and endothelial gene expression. Although ZStrC lacked the ability to differentiate into classic mesenchymal stromal cell lineages (i.e., osteocytes, adipocytes, chondrocytes), they did have the capacity fo... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487930 Wed, 14 Sep 2011 04:00:00 +0100 5487930 http://www.medworm.com/index.php?rid=5409659&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004498%2Fabstract%3Frss%3Dyes In this study, we explored the possible mechanism of bortezomib resistance in clinical MM patients. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409659 Wed, 14 Sep 2011 04:00:00 +0100 5409659 http://www.medworm.com/index.php?rid=5487926&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004516%2Fabstract%3Frss%3Dyes HSPB8 has been shown to be involved in regulation of cell proliferation and apoptosis, and it has also been found to have divergent properties in solid tumors. The purpose of this study was to investigate the expression and function of HSPB8 in hematopoietic malignancies. Expression and induced expression of HSPB8 was evaluated in hematopoietic tumor cell lines and bone marrow samples from patients with leukemia. Methylation status was investigated by methylation-specific polymerase chain reaction. The role of HSPB8 in hematopoietic malignancies was addressed by reintroducing HSPB8 expression into the K562 (leukemia) and Namalwa (lymphoma) cell lines. Expression of HSPB8 was absent in hematopoietic tumor cell lines and primary patient and normal volunteer samples. Promoter DNA methylation ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5487926 Mon, 12 Sep 2011 04:00:00 +0100 5487926 http://www.medworm.com/index.php?rid=5409665&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004450%2Fabstract%3Frss%3Dyes We report that the pan-leukocyte CD45 phosphatase plays an essential role in trafficking and repopulation of the bone marrow by immature human CD34+ cells and leukemic cells in transplanted nonobese diabetic severe combined immunodeficient mice. Inhibiting CD45 function by blocking antibodies or a CD45 inhibitor impaired the motility of both normal and leukemic human cells. Blocking CD45 inhibited homing and repopulation by immature human CD34+ cells as well as homing of primary patient leukemic cells. In addition, CD45 inhibition negatively affected development of hematopoietic progenitors in vitro and their recovery in transplanted recipients in vivo, revealing the central role of CD45 in the regulation of hematopoiesis. Moreover, CD45 blockage induced a hyperadhesive phenotype in imma... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409665 Mon, 12 Sep 2011 04:00:00 +0100 5409665 http://www.medworm.com/index.php?rid=5409663&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004504%2Fabstract%3Frss%3Dyes Telomere maintenance, important for long-term cell survival and malignant transformation, is directed by a multitude of factors, including epigenetic mechanisms, and has been implicated in outcomes for patients with leukemia. In the present study, the objective was to investigate the biological and clinical significance of telomere length and promoter methylation of the human telomerase reverse transcriptase gene in childhood acute lymphoblastic leukemia. A cohort of 169 childhood acute lymphoblastic leukemias was investigated for telomere length, human telomerase reverse transcriptase gene promoter methylation status, genomic aberrations, immunophenotype, and clinical outcomes. Methylation of the core promoter of the human telomerase reverse transcriptase (hTERT) gene was demonstrated in ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409663 Mon, 12 Sep 2011 04:00:00 +0100 5409663 http://www.medworm.com/index.php?rid=5409662&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004462%2Fabstract%3Frss%3Dyes Severe congenital neutropenia is associated with a marked propensity to develop myelodysplasia or acute myeloid leukemia (AML). Truncation mutations of CSF3R, encoding the granulocyte colony-stimulating factor receptor (G-CSFR), are associated with development of myelodysplasia/AML in severe congenital neutropenia. However, a causal relationship between CSF3R mutations and leukemic transformation has not been established. Herein, we show that truncated G-CSFR cooperates with the PML-RARα oncogene to induce AML in mice. Expression of truncated G-CSFR significantly shortens the latency of AML in a G-CSF−dependent fashion and it is associated with a distinct AML presentation characterized by higher blast counts and more severe myelosuppression. Basal and G-CSF−induced signal transducer ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409662 Mon, 12 Sep 2011 04:00:00 +0100 5409662 http://www.medworm.com/index.php?rid=5409660&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004486%2Fabstract%3Frss%3Dyes We report a patient who developed donor-derived MDS followed by leukemic transformation after cord blood transplantation for MDS with MG. Interestingly, MG reappeared before development of donor-derived MDS. We analyzed the immunoglobulin allotype gene polymorphisms to determine whether the MG after cord blood transplantation was of recipient origin or donor origin. Results of genetic analysis and enzyme-linked immunosorbent assay of IgG1 allotype revealed that the MG after cord blood transplantation was of donor origin. Although the mechanism of donor-derived MG remains unclear, the persistent presence of recipient’s antigen presenting cells might have induced the abnormal immunoglobulin production. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409660 Mon, 12 Sep 2011 04:00:00 +0100 5409660 http://www.medworm.com/index.php?rid=5409661&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004401%2Fabstract%3Frss%3Dyes We recently re-established a line of sheep that accurately mimics the clinical symptoms and genetics of severe hemophilia A (HA). Here, we tested a novel, nonablative transplantation therapy in two pediatric HA animals. Paternal mesenchymal stem cells (MSC) were transduced with a porcine FVIII-encoding lentivector and transplanted via the intraperitoneal route without preconditioning. At the time of transplantation, these animals had received multiple human FVIII treatments for various spontaneous bleeds and had developed debilitating hemarthroses, which produced severe defects in posture and gait. Transplantation of transduced MSC resolved all existent hemarthroses, and spontaneous bleeds ceased. Damaged joints recovered fully; the animals regained normal posture and gait and resumed norm... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409661 Thu, 08 Sep 2011 04:00:00 +0100 5409661 http://www.medworm.com/index.php?rid=5239107&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004139%2Fabstract%3Frss%3Dyes See Chung et al., pages 986–998. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239107 Fri, 26 Aug 2011 04:00:00 +0100 5239107 http://www.medworm.com/index.php?rid=5324647&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004073%2Fabstract%3Frss%3Dyes Expanding highly cytotoxic lymphokine activated natural killer cells and cytokine induced killer T cells by Good Manufacturing Practice-quality cultures is an important step toward effective use of those killer cells to treat acute myeloid leukemia patients receiving stem cell transplantation. Elucidating how those killer cells recognize leukemia stem cells will be the next challenge. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324647 Thu, 25 Aug 2011 04:00:00 +0100 5324647 http://www.medworm.com/index.php?rid=5409666&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004115%2Fabstract%3Frss%3Dyes Various agents, including chemotherapeutic drugs, can induce cell senescence. However, the mechanisms involved in the aging pathway, particularly the stress that chemotherapy imposes on telomeres, are still undefined. To address these issues, human mesenchymal stem cells (MSCs) were assessed as target cells to investigate the initiation of the aging process by chemotherapy. The MSCs were obtained from bone marrow (BM) cells from normal adults and grown in the presence of platelet lysates. Cultured MSCs were identified for immunophenotype, and for growth and differentiation properties. The MSCs were exposed to 10 nM doxorubicin and 500 ng/mL etoposide, sublethal doses that induce DNA double-stranded breaks. Telomere length (TL) was assessed by flow-fluorescence in situ hybridization and Sou... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409666 Mon, 22 Aug 2011 04:00:00 +0100 5409666 http://www.medworm.com/index.php?rid=5409664&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004127%2Fabstract%3Frss%3Dyes In conclusion, future clinical trials should favor CD4+CD25hiCD127− and CD4+CD25hiICOS+ Tregs for direct Treg cell transfer, whereas CD4+CD25hi Tregs qualify as best candidate for in vitro expansion. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5409664 Mon, 22 Aug 2011 04:00:00 +0100 5409664 http://www.medworm.com/index.php?rid=5324652&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100405X%2Fabstract%3Frss%3Dyes Transient downregulation of genes in vitro employing short interfering RNA (siRNA) is a time-honored approach to study gene function. A crucial prerequisite to obtain a downregulation is an efficient and nontoxic delivery of the siRNA into the target cells. However, this has proven difficult to accomplish, particular in cells in suspension. Thus, there is a need for a systematic evaluation of different methodologies to identify the most suitable protocol. We compared Nucleofection with Accell, a novel nonviral-based delivery system in the setting of leukemic blasts from patients with myeloid leukemias. Two cell surface proteins, human inhibitory C-type lectin-like receptor and CD96, both believed to be associated with leukemic stem cells, were chosen as target genes. Accell not only yield... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324652 Mon, 22 Aug 2011 04:00:00 +0100 5324652 http://www.medworm.com/index.php?rid=5324651&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004103%2Fabstract%3Frss%3Dyes We present the first studies on microvesicle production by excised cancers from human lung and the capacity of these microvesicles to alter the genetic phenotype of normal human marrow cells. We studied 12 cancers involving the lung and assessed nine lung-specific mRNA species (aquaporin, surfactant families, and clara cell-specific protein) in marrow cells exposed to tissue in co-culture, cultured in conditioned media, or exposed to isolated lung cancer-derived microvesicles. We assessed two or seven days of co-culture and marrow which was unseparated, separated by ficoll density gradient centrifugation or ammonium chloride lysis. Under these varying conditions, each cancer derived from lung mediated marrow expression of between one and seven lung-specific genes. Microvesicles were identi... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324651 Mon, 22 Aug 2011 04:00:00 +0100 5324651 http://www.medworm.com/index.php?rid=5324650&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004085%2Fabstract%3Frss%3Dyes Chemoimmunotherapy with anti-CD20 monoclonal antibody rituximab is increasingly used for the treatment of patients with chronic lymphocytic leukemia (CLL). Antibody-dependent cytotoxicity (ADCC) is one of the most important mechanisms of action of rituximab against B-cell malignancies. We studied ways to increase the cytotoxic effect of rituximab on CLL cells by enhancing ADCC. Peripheral blood mononuclear cell (PBMC) or purified natural killer (NK) cells from healthy donors were activated with interleukin-15 (IL-15) and cultured with rituximab-coated CLL cells, and ADCC was evaluated using a 51chromium release assay. The IL-15 significantly enhanced in vitro ADCC against CLL cells, and this effect was mainly mediated by NK cells. The IL-15 treated effector cells with the low affinity Fc�... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324650 Mon, 22 Aug 2011 04:00:00 +0100 5324650 http://www.medworm.com/index.php?rid=5324649&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004061%2Fabstract%3Frss%3Dyes Recent evidence suggested that 5-azacytidine (5-aza) can impact important immune functions via epigenetic modifications, making it an attractive candidate for pharmacologic manipulation of the immune system. The aim of this work was to study the effects of 5-aza on human dendritic cells (DC) generated from peripheral blood monocytes, and to test the type of immune response induced in patients treated with 5-aza. On the phenotypic level, CD40 and CD86 expression was significantly increased on mature DC exposed to 5-aza (5-aza-DC), compared with control untreated DC. Mature control DC and mature 5-aza-DC secreted comparable amounts of interleukin (IL)-6, IL-12p70, IL-23, and tumor necrosis factor−α. However, mature 5-aza-DC secreted significantly lower levels of IL-10 and IL-27 compared t... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324649 Mon, 22 Aug 2011 04:00:00 +0100 5324649 http://www.medworm.com/index.php?rid=5324648&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004097%2Fabstract%3Frss%3Dyes In conclusion, PSGL-1 microparticles show a characteristic course during alloSCT and their possible association with relapse/progress requires further evaluation of the PSGL-1/P-selectin interaction in leukemias and lymphomas. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324648 Mon, 22 Aug 2011 04:00:00 +0100 5324648 http://www.medworm.com/index.php?rid=5324656&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11004036%2Fabstract%3Frss%3Dyes In the article by Jung et al., entitled “Annexin-2 is a regulator of stromal cell-derived factor-1/CXCL12 function in the hematopoietic stem cell endosteal niche,” which appeared in the February 2011 issue of Experimental Hematology (Vol. 39, Issue 2, Pages 151-166), D erroneously replicated the data that appears as F. Here is the corrected data for D appears on right side. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324656 Tue, 16 Aug 2011 04:00:00 +0100 5324656 http://www.medworm.com/index.php?rid=5120516&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003663%2Fabstract%3Frss%3Dyes See Erdogan et al., pages 915–926. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120516 Wed, 27 Jul 2011 23:00:00 +0100 5120516 http://www.medworm.com/index.php?rid=5239115&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003651%2Fabstract%3Frss%3Dyes Conclusions: miR-10a expression is highly characteristic for AML (IR) patients with NPM1 mutations and may influence its biological properties in AML by interfering with the p53 machinery partly regulated by MDM4. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239115 Mon, 25 Jul 2011 04:00:00 +0100 5239115 http://www.medworm.com/index.php?rid=5324654&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003638%2Fabstract%3Frss%3Dyes Conclusions: These investigations indicate that early and preclinical expression of CCL8 in plasma predicts overall survival of GVHD mice. Together with an involvement of allo-recognition in CCL8 expression, it suggests that CCL8 plays an important role in GVHD pathology. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324654 Fri, 22 Jul 2011 04:00:00 +0100 5324654 http://www.medworm.com/index.php?rid=5324653&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100364X%2Fabstract%3Frss%3Dyes Conclusions: Together our results indicate that during embryonic hematopoietic differentiation Mxd4 is an important player in the regulation of blood progenitor proliferation, and suggest that downregulation of its expression might be required for a proliferative burst preceding lineage specification. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5324653 Fri, 22 Jul 2011 04:00:00 +0100 5324653 http://www.medworm.com/index.php?rid=5239108&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003626%2Fabstract%3Frss%3Dyes One of the key challenges facing biomedical research is to extract biologically meaningful information from the ever-increasing scale and complexity of datasets generated through high-throughput approaches. Hematopoiesis represents one of the most experimentally tractable mammalian organ systems and, therefore, has historically tended to be at the forefront of applying new technologies within biomedical research. The combination of massive parallel sequencing technologies with chromatin-immunoprecipitation (ChIP-Seq) permits genome-scale characterization of histone modification status and identification of the complete set of binding sites for transcription factors. Because transcription factors have long been recognized as essential regulators of cell fate choice in hematopoiesis, ChIP-Se... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239108 Thu, 21 Jul 2011 04:00:00 +0100 5239108 http://www.medworm.com/index.php?rid=5239114&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003316%2Fabstract%3Frss%3Dyes Conclusions: The higher radiation dose (1300 vs. 900 cGy) resulted in significantly enhanced GVL effect against an extramedullary tumor and the alterations in effector T-cell trafficking into tumor tissue are the most likely mechanism. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239114 Mon, 18 Jul 2011 04:00:00 +0100 5239114 http://www.medworm.com/index.php?rid=5239113&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11003304%2Fabstract%3Frss%3Dyes Conclusions: SNDX-275 has antiproliferative activity in HL cell lines, involving several mechanisms: induction of apoptosis, regulation of cytokines and chemokines, and alteration of cancer/testis antigens. Clinical investigation of SNDX-275 alone or in combination with Bcl-2 inhibitors is warranted in patients with HL. Phase 2 studies with SNDX-275 in HL are ongoing, and future clinical studies should investigate combinations with SNDX-275. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239113 Mon, 18 Jul 2011 04:00:00 +0100 5239113 http://www.medworm.com/index.php?rid=5064342&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002918%2Fabstract%3Frss%3Dyes See Matsuki et al., pages 829–836. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064342 Tue, 05 Jul 2011 23:00:00 +0100 5064342 http://www.medworm.com/index.php?rid=5239112&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002876%2Fabstract%3Frss%3Dyes Conclusions: Resveratrol exerts its chemotherapeutic effect on human MM cells through mechanisms involving the impairment of the pro-survival XBP1 signaling and the activation of pro-apoptotic ER stress response. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239112 Mon, 04 Jul 2011 04:00:00 +0100 5239112 http://www.medworm.com/index.php?rid=5239111&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002906%2Fabstract%3Frss%3Dyes Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239111 Mon, 04 Jul 2011 04:00:00 +0100 5239111 http://www.medworm.com/index.php?rid=5239110&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002888%2Fabstract%3Frss%3Dyes Conclusions: Although nonmyeloablative conditioning reversed the autoimmune process in prediabetic NOD mice, the same regimen was significantly less effective in establishing chimerism and reversing autoimmune diabetes in spontaneously diabetic NOD mice. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239110 Mon, 04 Jul 2011 04:00:00 +0100 5239110 http://www.medworm.com/index.php?rid=5239109&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100289X%2Fabstract%3Frss%3Dyes This study elucidates varied mast cell physiological functions for KSR1, including those related to cytoskeletal organization, and it suggests a novel molecular target for attenuating mast cell−mediated inflammation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5239109 Mon, 04 Jul 2011 04:00:00 +0100 5239109 http://www.medworm.com/index.php?rid=5120523&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002827%2Fabstract%3Frss%3Dyes Conclusions: Our current study demonstrates a positive impact of Bid on hematopoietic regeneration mainly due to its unique effects on donor lymphopoiesis in the transplant recipients. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120523 Sun, 19 Jun 2011 23:00:00 +0100 5120523 http://www.medworm.com/index.php?rid=5120518&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002839%2Fabstract%3Frss%3Dyes Conclusions: Clinical scale expansion of CIK cells is feasible from both healthy donors and leukemia patients at various stages of treatment. This robust system allows clinical translation using CIK cells as immunotherapy in various clinical settings. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120518 Sun, 19 Jun 2011 23:00:00 +0100 5120518 http://www.medworm.com/index.php?rid=5120517&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002840%2Fabstract%3Frss%3Dyes Rituximab is one of the most commonly used drugs in the treatment of B-cell non-Hodgkin lymphoma. Because of its ability to target CD20+ lymphocytes, its use before allogeneic stem cell transplantation seemed to reduce risk of graft-vs.-host disease (GVHD) occurrence. We retrospectively analyzed the outcomes of adult patients diagnosed with CD20+ lymphoproliferative disease undergoing allogeneic stem cell transplantation and receiving, or not receiving, rituximab up to 3 months before transplantation. Analysis on a cohort of 57 patients showed a protective role of rituximab on the occurrence of acute GVHD for those receiving anti-thymocyte globulin during conditioning (n = 39). Grade 2 to 4 and 3 to 4 acute GVHD occurred in 10% vs. 48% (p = 0.03) and 0% vs. 24% (p = 0.08) in the rituximab ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120517 Sun, 19 Jun 2011 23:00:00 +0100 5120517 http://www.medworm.com/index.php?rid=5120520&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002785%2Fabstract%3Frss%3Dyes Conclusions: Our data utilize both discovery and validation sets and two complementary platforms to identify miRNAs associated with MDS. We have analyzed predicted targets and identified c-Myb and Sufu as potential diagnostic markers of MDS. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120520 Wed, 15 Jun 2011 23:00:00 +0100 5120520 http://www.medworm.com/index.php?rid=5120522&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002773%2Fabstract%3Frss%3Dyes Conclusions: These results suggest that Ly6C+ monocytes can become HpSCs in the injured liver. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120522 Tue, 14 Jun 2011 23:00:00 +0100 5120522 http://www.medworm.com/index.php?rid=5120521&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002761%2Fabstract%3Frss%3Dyes Conclusions: This study indicates that TLR9 triggering on B cells influences their interaction with extracellular matrix, which will be critical in modulating activation of these cells in conditions, such as infections, and gives a basic insight into the contribution of innate immunity elements in B-cell functional responses. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120521 Sun, 12 Jun 2011 23:00:00 +0100 5120521 http://www.medworm.com/index.php?rid=5120519&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002797%2Fabstract%3Frss%3Dyes Conclusions: Our results demonstrate the potential for IL-15 to support large-scale expansion of clinical-grade LAK-NK effectors, which could retain enhanced longer-term potency and preserve activation receptors in therapy of hematological malignancies. Protocols are readily clinically translatable. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5120519 Sun, 12 Jun 2011 23:00:00 +0100 5120519 http://www.medworm.com/index.php?rid=5064344&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001895%2Fabstract%3Frss%3Dyes Conclusions: Our data suggest that Bcl-2 is a better therapeutic target than c-Myc, but attacking both Bcl-2 and c-Myc would be an even more effective treatment strategy for diffuse large B-cell lymphomas with concurrent Bcl-2 and c-Myc overexpression. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064344 Wed, 01 Jun 2011 23:00:00 +0100 5064344 http://www.medworm.com/index.php?rid=5064350&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002372%2Fabstract%3Frss%3Dyes Conclusions: We propose here a novel graft-versus-GVHD treatment to treat refractory GVHD, and these results strongly suggest that GVHD can be successfully treated by eliminating the harmful lymphocytes responsible for GVHD by a second allogeneic stem cell transplantation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064350 Sun, 29 May 2011 23:00:00 +0100 5064350 http://www.medworm.com/index.php?rid=5064349&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002384%2Fabstract%3Frss%3Dyes Conclusions: These results reveal new candidate positive regulators of human PS cells, providing evidence of their ability to regulate expression of pluripotency, cell cycle, and differentiation genes in human PS cells. These data provide valuable new leads for further elucidating mechanisms of human pluripotency. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064349 Sun, 29 May 2011 23:00:00 +0100 5064349 http://www.medworm.com/index.php?rid=5064348&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002396%2Fabstract%3Frss%3Dyes Conclusions: We provided evidence that KIT p.N822I mutation has transforming potential and can cause a constitutive activation of KIT. In addition, we demonstrated that KIT-N822I is resistant to imatinib and sensitive to dasatinib. Finally, our findings support the hypothesis that not only KIT mutations but other additional genetic abnormalities are contributing to more advanced forms of the disease. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064348 Sun, 29 May 2011 23:00:00 +0100 5064348 http://www.medworm.com/index.php?rid=5064347&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11002360%2Fabstract%3Frss%3Dyes Conclusions: ECP is cytotoxic for HL tumor cells even at low concentrations, but heterogeneity between cell lines exists and not all tumor cells are eradicated. Two cell lines of B-cell origin, KMH2 and L428, were sensitive to ECP in a dose−response manner, but for HDLM-2, which is of T-cell origin, the cytotoxicity reached a plateau. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064347 Wed, 25 May 2011 23:00:00 +0100 5064347 http://www.medworm.com/index.php?rid=5064346&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100227X%2Fabstract%3Frss%3Dyes Conclusions: Elevation of the inflammatory cytokine IFN-γ−stimulated MHC class II expression and MHC class II-Fas colocalization, which may facilitate T-cell−mediated cell destruction. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064346 Sun, 15 May 2011 23:00:00 +0100 5064346 http://www.medworm.com/index.php?rid=5064343&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001871%2Fabstract%3Frss%3Dyes Interactions between hematopoiesis and bone metabolism have been described in various developmental and pathological situations. Here we review this evidence from the literature with a focus on microenvironmental regulation of hematopoiesis and bone metabolism. Our hypothesis is that this process occurs by bidirectional signaling between hematopoietic and mesenchymal cells through cell adhesion molecules, membrane-bound growth factors, and secreted matrix proteins. Examples of steady-state hematopoiesis and pathologies are presented and support our view that hematopoietic and mesenchymal cell functions are modulated by specific microenvironments in the bone marrow. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064343 Tue, 10 May 2011 23:00:00 +0100 5064343 http://www.medworm.com/index.php?rid=5064345&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001883%2Fabstract%3Frss%3Dyes Conclusions: We found that huVB22B, eltrombopag, and thrombopoietin have different effects on megakaryopoiesis, platelet function, and intracellular signaling. The precise mechanisms for these different biological effects regarding stimulation through the same receptor, Mpl, remain to be elucidated. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=5064345 Sun, 08 May 2011 23:00:00 +0100 5064345 http://www.medworm.com/index.php?rid=4865138&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100186X%2Fabstract%3Frss%3Dyes See Walenda et al., pages 617–628 (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865138 Thu, 28 Apr 2011 23:00:00 +0100 4865138 http://www.medworm.com/index.php?rid=4865139&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001810%2Fabstract%3Frss%3Dyes In a previous editorial, I commented on my sense that this is an interesting time for our field and our Journal. This is reinforced by some recent changes in our editorial office and by news garnered at a recent meeting of Editors hosted by our publisher, Elsevier. First to the point of the editorial office, I would like to congratulate Ms. Kim Higgins, who has left her role as Managing Editor to take on a challenging new opportunity with the academic office at the University of Nevada, Reno. We wish her great success in her new role and thank her for her outstanding efforts for our Journal during Dr. Zanjani’s editorship. After a careful search, we were fortunate to recruit Dr. Carolina Abramovich to this position, which is now located in Vancouver. Dr. Abramovich holds a Ph.D. in Bioch... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865139 Sun, 24 Apr 2011 23:00:00 +0100 4865139 http://www.medworm.com/index.php?rid=4865148&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100172X%2Fabstract%3Frss%3Dyes Conclusions: Our findings implicate Fbxl10 in the maintenance of self-renewal capacity of HSCs, thus highlight a role of histone demethylation for the first time in the epigenetic regulation of HSCs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865148 Mon, 11 Apr 2011 23:00:00 +0100 4865148 http://www.medworm.com/index.php?rid=4764258&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001329%2Fabstract%3Frss%3Dyes See Hannah et al., pages 531–541. Hannah et al report a new valuable resource called the Hemo-ChIP Compendium, in which they integrated data from 53 ChIP-Seq experiments, covering 13 different haematopoietic cell types and 30 different factors relevant to transcriptional control of mouse haematopoiesis. They generated very comprehensive and user friendly data files which will ensure broad utility in the haematopoiesis research community as they will allow the average experimental biologist to easily gain access to a genome-wide map of haematopoietic transcription factor binding. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764258 Wed, 30 Mar 2011 23:00:00 +0100 4764258 http://www.medworm.com/index.php?rid=4865145&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001330%2Fabstract%3Frss%3Dyes Conclusions: Our results show dysregulated mitochondrial gene expression that goes beyond a simple age-related effect and is compatible with the putative role of mitochondrial dysfunction in MDS pathophysiology. However, it remains unclear whether the problem arises from primary lesions in the mitochondria, i.e., mitochondrial DNA mutations, or as a result of changes in the cell nucleus. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865145 Sun, 27 Mar 2011 23:00:00 +0100 4865145 http://www.medworm.com/index.php?rid=4764271&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001317%2Fabstract%3Frss%3Dyes There is an error in the presentation of data in Table 2 in the recently published article (Exp Hematol. 2011;39:305-320) entitled “A novel ENU-generated truncation mutation lacking the spectrin-binding and C-terminal regulatory domains of Ank1 models severe hemolytic hereditary spherocytosis” by Michael R. Hughes et al. Data in column 4 (Nonaffected, WT) refers to the peripheral blood phenotype of nonaffected, wild type mice of the hybrid strain, not the C3H/HeJ (N8-9) strain as shown. The correct version of appears below. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764271 Sun, 27 Mar 2011 23:00:00 +0100 4764271 http://www.medworm.com/index.php?rid=4865147&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001305%2Fabstract%3Frss%3Dyes Conclusions: Adipose tissue is one of the alternative sources of MSCs to bone marrow. We showed that AT-MSCs overexpressing CXCR4 preserve their ability for osteodifferentiation. Enhanced migration and engraftment of the transduced AT-MSCs into bone marrow indicate the usefulness of this strategy in overcoming low engraftment of MSCs in clinical approaches of cellular therapies for bone disorders and can represent a powerful tool in regenerative medicine and gene therapies. Thus, these cells may be used as an alternative to bone marrow−derived MSCs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865147 Wed, 23 Mar 2011 00:00:00 +0100 4865147 http://www.medworm.com/index.php?rid=4865143&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001299%2Fabstract%3Frss%3Dyes Conclusions: Foamy virus vectors expressing the human gp91phox transgene constitute potential candidates for the gene therapy of CGD because they combine lack of pathogenicity with efficacy even at low MOI. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865143 Wed, 23 Mar 2011 00:00:00 +0100 4865143 http://www.medworm.com/index.php?rid=4865142&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001287%2Fabstract%3Frss%3Dyes Conclusions: Taken together, these results suggest that LYL-1 plays a definite role in erythropoiesis, albeit with different effects in BM specifically regulating basal erythropoiesis, and spleen, controlling stress-induced erythropoiesis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865142 Mon, 21 Mar 2011 00:00:00 +0100 4865142 http://www.medworm.com/index.php?rid=4865146&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001226%2Fabstract%3Frss%3Dyes Conclusions: Our results suggest that activation of anti-rat platelet T-helper cells can bypass the mechanism of tolerance and result in the secretion of autoreactive antibodies, but this response is still controlled by regulatory T cells that develop progressively after immunization. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865146 Mon, 14 Mar 2011 00:00:00 +0100 4865146 http://www.medworm.com/index.php?rid=4865149&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001214%2Fabstract%3Frss%3Dyes Conclusions: Manipulating host immature DCs may represent a novel mechanism by which bortezomib improves aGVHD. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865149 Wed, 09 Mar 2011 00:00:00 +0100 4865149 http://www.medworm.com/index.php?rid=4865144&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11001202%2Fabstract%3Frss%3Dyes Conclusions: These results demonstrate that SphK1 is upregulated in imatinib-resistant K562 cells by a pathway contingent on a phosphoinositide 3-kinase/AKT2/mammalian target of rapamycin signaling pathway. We propose that SphK1 plays an important role in development of acquired resistance to imatinib in chronic myeloid leukemia cell lines. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865144 Wed, 09 Mar 2011 00:00:00 +0100 4865144 http://www.medworm.com/index.php?rid=4865141&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000774%2Fabstract%3Frss%3Dyes Conclusions: The supportive effect of MSCs on hematopoiesis can be significantly increased by addition of specific recombinant growth factors; especially in combination with SCF, TPO, and FGF-1. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865141 Mon, 28 Feb 2011 00:00:00 +0100 4865141 http://www.medworm.com/index.php?rid=4764272&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000786%2Fabstract%3Frss%3Dyes On January 20, 2011, the interwoven fields of stem cell biology, hematology, and leukemia said goodbye to Dr. Ernest A. McCulloch, one of their most creative and insightful champions. Affectionately known to his closest colleagues and trainees as “Bun,” he was the inspiring parent of many of the concepts that have laid the foundation for current and future advances in these areas of science and medicine. Dr. McCulloch was born in 1926 in Toronto, where he also grew up and went to University, graduating as a physician in 1948. After an introduction to research at the Lister Institute in London, England, he became a member of the faculty of the Ontario Cancer Institute in 1957. There he met and soon teamed up with Dr. James Till, a recently appointed physicist turned biophysicist with a... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764272 Mon, 28 Feb 2011 00:00:00 +0100 4764272 http://www.medworm.com/index.php?rid=4764270&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100066X%2Fabstract%3Frss%3Dyes In the article entitled “Timing of Captopril Administration Determines Radiation Protection or Radiation Sensitization in a Murine Model of Total Body Irradiation” by Davis et al. (Exp Hematol. 2010;38:270−281), the authors wish to make the following correction of the units for captopril administration to mice. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764270 Mon, 28 Feb 2011 00:00:00 +0100 4764270 http://www.medworm.com/index.php?rid=4645565&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000725%2Fabstract%3Frss%3Dyes Eugene Goldwasser, who has made a lasting contribution to science and medicine, died from complications related to advanced prostate cancer at his home in Chicago on Friday December 17th, 2010. Generally regarded as the “father of EPO,” Gene's laboratory at the University of Chicago succeeded, after 25 years of focused effort, in purifying firstly erythropoietin (EPO) from sheep and then human EPO. These achievements helped to launch the biotechnology revolution in the 1980s and improved the quality of life for millions of patients suffering from kidney disease and other anemic disorders. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645565 Mon, 28 Feb 2011 00:00:00 +0100 4645565 http://www.medworm.com/index.php?rid=4645552&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000749%2Fabstract%3Frss%3Dyes See Pardee et al, pages 473-485. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645552 Wed, 23 Feb 2011 00:00:00 +0100 4645552 http://www.medworm.com/index.php?rid=4764263&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000737%2Fabstract%3Frss%3Dyes Conclusions: The ChIP-Seq compendium described here provides a valuable resource for the wider research community by accelerating the discovery of transcriptional mechanisms operating in the hematopoietic system. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764263 Mon, 21 Feb 2011 00:00:00 +0100 4764263 http://www.medworm.com/index.php?rid=4764261&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000683%2Fabstract%3Frss%3Dyes We thank Dr. Moulder and colleagues for their interest in our recent work, “Timing of Captopril Administration Determines Radiation Protection or Radiation Sensitization in a Murine Model of Total Body Irradiation” . In our article, we demonstrated that the timing of captopril treatment relative to exposure to total body irradiation (TBI) differentially affects the viability and repopulation capacity of spared hematopoietic stem cells, resulting in either radiation protection or radiation sensitization. Captopril treatment beginning 1 hour post-irradiation and continuing for 30 days resulted in 100% survival from a radiation dose that in untreated mice results in only 50% survival after 30 days (LD50/30), with improved recovery of mature blood cells and multilineage hematopoietic proge... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764261 Thu, 17 Feb 2011 00:00:00 +0100 4764261 http://www.medworm.com/index.php?rid=4764260&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000671%2Fabstract%3Frss%3Dyes We read with great interest the article by Davis et al. , which reported that the angiotensin-converting enzyme (ACE) inhibitor captopril dramatically increased radiation-induced hematopoietic lethality when used in mice before 7.5 Gy total body irradiation (TBI) and dramatically decreased it when given after TBI. Davis et al. note that we found that “captopril failed to provide bone marrow protection in rats when administration was initiated 7 days prior to irradiation and continued for 28 days after irradiation.” It is correct that we found no effect, beneficial or detrimental, in this schedule in rats, but the possibility arose that in our 1993 study , a beneficial effect of pre-TBI therapy was negated by a detrimental effect of post-TBI therapy. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764260 Thu, 17 Feb 2011 00:00:00 +0100 4764260 http://www.medworm.com/index.php?rid=4764265&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000634%2Fabstract%3Frss%3Dyes Conclusions: This study provides an overview of expression data related to ADAMs and ADAMTSs genes potentially involved in myeloma pathogenesis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764265 Mon, 14 Feb 2011 00:00:00 +0100 4764265 http://www.medworm.com/index.php?rid=4764264&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000646%2Fabstract%3Frss%3Dyes Conclusions: Identical mutations of the JAK2 gene occur in humans and dogs, giving rise to a constitutively active JAK2 kinase, suggesting a common mechanism for human and canine diseases. Thus, common diagnostic tools and therapeutic approaches may be relevant. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764264 Mon, 14 Feb 2011 00:00:00 +0100 4764264 http://www.medworm.com/index.php?rid=4764262&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000658%2Fabstract%3Frss%3Dyes Conclusions: Ablation of the A2bAR in activated macrophages increases MMP-9. A2bAR activation reduces MMP-9 expression, which depends on TNF-α and could contribute to the protective role of A2bAR in a vascular injury model. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764262 Mon, 14 Feb 2011 00:00:00 +0100 4764262 http://www.medworm.com/index.php?rid=4764269&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000245%2Fabstract%3Frss%3Dyes Conclusions: Lnk is a negative regulator of PDGFR signaling. Development of Lnk mimetic drugs might provide a novel therapeutic strategy for myeloproliferative disorders. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764269 Wed, 09 Feb 2011 00:00:00 +0100 4764269 http://www.medworm.com/index.php?rid=4764267&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000233%2Fabstract%3Frss%3Dyes Conclusions: We report for the first time a human TEC line that supports lymphopoiesis from cord blood and bone marrow HPC. The TEC cell lines described herein provide a novel human thymic stroma model to study the contribution of human leukocyte antigen molecules and Notch ligands to T-cell commitment and maturation and could be utilized to promote lymphopoiesis for immune cell therapy. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764267 Tue, 08 Feb 2011 00:00:00 +0100 4764267 http://www.medworm.com/index.php?rid=4764266&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000221%2Fabstract%3Frss%3Dyes Conclusion: Doxorubicin can increase expression of mdr1/P-glycoprotein through activating MAPK/ERK transduction pathway, then increasing expression of YB-1, inducing YB-1 nuclear translocation, and enhancing DNA-binding activity of YB-1. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764266 Tue, 08 Feb 2011 00:00:00 +0100 4764266 http://www.medworm.com/index.php?rid=4596507&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000142%2Fabstract%3Frss%3Dyes See Mohr et al, pages 272-281. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596507 Fri, 04 Feb 2011 00:00:00 +0100 4596507 http://www.medworm.com/index.php?rid=4764268&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100021X%2Fabstract%3Frss%3Dyes Conclusions: Our results indicate that endogenous CD26 normally suppresses megakaryopoiesis and that loss of CD26 activity results in expansion of the megakaryocyte progenitor population in vivo. This suggests the potential use of CD26 inhibitors to improve megakaryocyte progenitor function and/or reconstitution of the megakaryocyte cell population. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764268 Wed, 02 Feb 2011 00:00:00 +0100 4764268 http://www.medworm.com/index.php?rid=4645558&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000191%2Fabstract%3Frss%3Dyes Conclusions: Micronucleated reticulocyte loss after higher sublethal radiation exposures results from rapid depletion of erythroid progenitors and precursors. This injury reveals that erythroid colony-forming units and proerythroblasts constitute a particularly proapoptotic compartment within the erythron. We conclude that the functional transition of primary proerythroblasts to later-stage erythroid precursors is characterized by a shift from a proapoptotic to an antiapoptotic phenotype. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645558 Wed, 02 Feb 2011 00:00:00 +0100 4645558 http://www.medworm.com/index.php?rid=4865140&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000208%2Fabstract%3Frss%3Dyes MicroRNAs (miRNAs) are short non-coding RNAs involved in post-trascriptional regulation of gene expression and diverse biological activities. They are crucial for self-renewal and behavior of embryonic stem cells, but their role in mesenchymal stem cells has been poorly understood. Recently emerging evidence suggests that miRNAs are closely involved in controlling key steps of mesenchymal stem cell differentiation into certain cell lineages. This review focuses on miRNAs identified recently that regulate mesenchymal stem cell differentiation and other activities. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4865140 Tue, 01 Feb 2011 00:00:00 +0100 4865140 http://www.medworm.com/index.php?rid=4764259&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000178%2Fabstract%3Frss%3Dyes Hematopoietic stem cell (HSC) either stays in quiescence or proliferates toward differentiation for the production of mature blood cells, or toward self-renewal for giving rise to itself. In order to both maintain a supply of mature blood cells and not exhaust HSCs throughout the lifetime of an individual, under steady state, most HSCs remain quiescent and only a small number enter the cell cycle. Quiescence of HSCs is not only critical for protecting the stem cell compartment and sustaining stem cell pools over long periods, but it is also critical for protecting stem cells by minimizing their accumulation of replication-associated mutations. The balance between quiescence and proliferation is tightly controlled by both HSC-intrinsic and -extrinsic mechanisms. In recent years, through red... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4764259 Tue, 01 Feb 2011 00:00:00 +0100 4764259 http://www.medworm.com/index.php?rid=4645561&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100018X%2Fabstract%3Frss%3Dyes Conclusions: These data demonstrate that the Flt3-ITD confers sensitivity to Ara-C, but resistance to doxorubicin in a manner that depends on p53. Thus, patients with Flt3-ITD positive AML may not benefit from treatment with an anthracycline. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645561 Tue, 01 Feb 2011 00:00:00 +0100 4645561 http://www.medworm.com/index.php?rid=4596508&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000154%2Fabstract%3Frss%3Dyes I first plunged into the fascinating field of experimental hematology some 40 years ago as a “green as grass” graduate student at the Ontario Cancer Institute. Those were heady times. New clonal assays provided our first glimpse of the complexity of the hematopoietic system and gave birth to key concepts of stem cell biology. This was also the era of the first bold steps toward bone marrow transplantation. As we moved into the 1980s, advances in biochemical purification methods and the advent of recombinant DNA technology rapidly saw crude “witch's brews” of conditioned media give way to a plethora of recombinant growth factors, identification of their myriad receptors, and the unraveling of complex signal transduction pathways. The next decade saw a further explosion in recombinan... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596508 Mon, 31 Jan 2011 00:00:00 +0100 4596508 http://www.medworm.com/index.php?rid=4645560&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000130%2Fabstract%3Frss%3Dyes Conclusions: This study shows activation of mTOR signaling in primary T-ALL cells evolving in the leukemic bone marrow, and supports the inclusion of mTOR antagonists in current therapeutic regimens for this cancer. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645560 Thu, 27 Jan 2011 00:00:00 +0100 4645560 http://www.medworm.com/index.php?rid=4645559&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000117%2Fabstract%3Frss%3Dyes Conclusions: These studies provide new insight into drug transport proteins that may be involved in the in vivo absorption, cellular distribution, and elimination of hydroxyurea. Elucidation of hydroxyurea transcellular movement should improve our understanding of its pharmacokinetics and pharmacodynamics, and may help explain some of the interpatient drug variability observed in patients with sickle cell anemia. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645559 Mon, 24 Jan 2011 00:00:00 +0100 4645559 http://www.medworm.com/index.php?rid=4645564&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000105%2Fabstract%3Frss%3Dyes Conclusions: Our study supports the hypothesis that very small embryonic-like stem cells constitute a “mobile” pool of primitive/pluripotent stem cells that could be released from the BM into the peripheral blood under the influence of various physiological or pathological stimuli. In order to fully support that hBM- and leukapheresis product−derived very small embryonic-like stem cells are actually pluripotent, we are currently testing their ability to differentiate in vitro into cells from all three germ layers. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645564 Tue, 18 Jan 2011 00:00:00 +0100 4645564 http://www.medworm.com/index.php?rid=4645553&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000099%2Fabstract%3Frss%3Dyes The past 20 years of experience with umbilical cord blood transplantation have demonstrated that cord blood is effective in the treatment of a spectrum of diseases, including hematological malignancies, bone marrow failure, hemoglobinopathies, and inborn errors of metabolism. Cord blood can be obtained with ease and then safely cryopreserved for either public or private use without loss of viability. As compared to other unrelated donor cell sources, cord blood transplantation allows for greater human leukocyte antigen disparity without a corresponding increase in graft-vs.-host disease. Moreover, cord blood has a lower risk of transmitting infections by latent viruses and is less likely to carry somatic mutations than other adult cells. Recently, multiple populations of stem cells with pr... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645553 Tue, 18 Jan 2011 00:00:00 +0100 4645553 http://www.medworm.com/index.php?rid=4645557&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000026%2Fabstract%3Frss%3Dyes Conclusions: This study demonstrates the immunogenicity of autologous DCs that cross-present leukemia-associated antigens from autologous apoptotic leukemic cells in vivo in elderly patients with AML. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645557 Mon, 10 Jan 2011 00:00:00 +0100 4645557 http://www.medworm.com/index.php?rid=4645556&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000038%2Fabstract%3Frss%3Dyes Conclusions: Our studies have unexpectedly found that Mad2 haploinsufficiency is protective in the presence of a cycle-specific DNA synthesis agent in vivo, and Ape1/Ref-1 inhibitor in vitro. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645556 Mon, 10 Jan 2011 00:00:00 +0100 4645556 http://www.medworm.com/index.php?rid=4645555&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1100004X%2Fabstract%3Frss%3Dyes We are grateful for the letter from Professor Dirk van Bekkum. We have to admit that these articles, mostly published before we were even born and not readily available in the online public domains, had escaped our attention. However, in the last 10 years numerous articles have been published on the advantages of intrafemoral or intrabone marrow transplantation (reviewed in ). Especially the groups of John Dick and Susumu Ikehara have pioneered the method in xenogeneic and allogeneic transplantation models, respectively. In these models, where (residual) immunity is involved, it may be advantageous to use intrafemoral transplantation. These studies were the basis for renewed interest in the method. Indeed, we find no advantage for using intrafemoral transplantation in syngeneic models and... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645555 Mon, 10 Jan 2011 00:00:00 +0100 4645555 http://www.medworm.com/index.php?rid=4645554&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X11000051%2Fabstract%3Frss%3Dyes In the November issue of the Journal, van Os et al. report equal engraftment efficacy in mice by intrafemoral and intravenous (retro-orbital) administration of syngeneic bone marrow. The authors justify their investigations on the grounds that “It is thought that when cells are injected directly into the place where they need to be (in the bone marrow), they shortcut the homing process and are able to provide better and faster engraftment.” This thinking may have prevailed among the authors, but was relegated to the stack of disproven hypotheses nearly half a century ago . Equivalence of the intravenous and intraosseal injection routes is also repeatedly referred to and discussed in several handbooks . (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645554 Mon, 10 Jan 2011 00:00:00 +0100 4645554 http://www.medworm.com/index.php?rid=4645563&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006491%2Fabstract%3Frss%3Dyes Conclusions: Our results demonstrate that the CD47 pathway in ITP patients abnormally modulates platelet homeostasis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4645563 Wed, 05 Jan 2011 00:00:00 +0100 4645563 http://www.medworm.com/index.php?rid=4596515&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1000648X%2Fabstract%3Frss%3Dyes Conclusions: This study identifies differences in expression of several osteoclast and macrophage genes, as well as functional differences between PT- and OPN-adherent cells. We conclude that the OPN-adherent cells display osteoclast characteristics, especially with regard to expression of matrix-degrading enzymes, whereas the PT-adherent cell might represent a unique TRAP-positive multinucleated bone marrow macrophage implicated in immune recognition and phagocytosis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596515 Mon, 03 Jan 2011 00:00:00 +0100 4596515 http://www.medworm.com/index.php?rid=4596512&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006107%2Fabstract%3Frss%3Dyes Conclusions: The Ank1E924X strain provides a novel tool to study Ank1 and model HS. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596512 Wed, 29 Dec 2010 00:00:00 +0100 4596512 http://www.medworm.com/index.php?rid=4387018&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006065%2Fabstract%3Frss%3Dyes On November 17th, 2010, the members of the International Society of Hematology lost a talented and productive colleague and friend, Dr. Alan M. Gewirtz, the C. Willard Robinson Professor of Hematology and Oncology at the University of Pennsylvania School of Medicine, to lung cancer. I write this obituary on behalf of the Society and our Journal not only to recognize this loss, but also to celebrate Alan’s numerous achievements and endearing personal qualities. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387018 Wed, 29 Dec 2010 00:00:00 +0100 4387018 http://www.medworm.com/index.php?rid=4596516&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006090%2Fabstract%3Frss%3Dyes Conclusions: Spleen HSCs and BM HSCs are functionally similar, but their cycling behaviors differ. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596516 Mon, 27 Dec 2010 00:00:00 +0100 4596516 http://www.medworm.com/index.php?rid=4596518&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006089%2Fabstract%3Frss%3Dyes Conclusions: When plated at high cell densities on irradiated fetal-liver−derived stromal cells, BM cells from WT mice maintain their therapeutic potential for 4 weeks in culture, which is sufficient time for correction of a faulty gene by targeting. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596518 Thu, 23 Dec 2010 00:00:00 +0100 4596518 http://www.medworm.com/index.php?rid=4596514&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006077%2Fabstract%3Frss%3Dyes Conclusions: Plk1 inhibition leads to cell death in MM cell lines and patient myeloma samples. Our data suggest that inhibition of Plk1 may have potential use as a therapeutic strategy in multiple myeloma. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596514 Thu, 23 Dec 2010 00:00:00 +0100 4596514 http://www.medworm.com/index.php?rid=4596519&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006041%2Fabstract%3Frss%3Dyes Conclusions: Our finding that AMD3100 rapidly mobilizes hematopoietic progenitor cells from the bone marrow into the periphery in CD26-deficient transgenic mice that otherwise exhibit a mobilization defect in response to G-CSF suggests that: (1) CD26 is downstream of G-CSF but upstream of the CXCL12-CXCR4 axis and (2) AMD3100 can be used as a single agent to mobilize hematopoietic stem and progenitor cells in normal donors or patients that have an intrinsic defect in their response to G-CSF treatment. Stem cell transplants are often the only curative treatment in some cancer patients. The ability to perform the transplantation and its success is dependent on the ability to mobilize adequate numbers of hematopoietic progenitor cells. The use of AMD3100 as a single agent would give patients ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596519 Mon, 20 Dec 2010 00:00:00 +0100 4596519 http://www.medworm.com/index.php?rid=4596509&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10006053%2Fabstract%3Frss%3Dyes Stem cells are defined as cells that have the ability to perpetuate themselves through self-renewal and to generate functional mature cells by differentiation. During each stage, coordinated gene expression is crucial to maintain the balance between self-renewal and differentiation. Disturbance of this accurately balanced system can lead to a variety of malignant disorders. In mammals, DNA cytosine-5 methylation is a well-studied epigenetic pathway that is catalyzed by DNA methyltransferases and is implicated in the control of balanced gene expression, but also in hematological malignancies. In this review, we focus on the TET (ten-eleven-translocation) genes, which recently were identified to catalyze the conversion of cytosine-5 methylation to 5-hydroxymethyl-cytosine, an intermediate ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596509 Mon, 20 Dec 2010 00:00:00 +0100 4596509 http://www.medworm.com/index.php?rid=4596511&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005977%2Fabstract%3Frss%3Dyes Conclusions: In nonirradiated mice, captopril biphasically regulates EPO via HIF activation. TBI ablates erythroid progenitors, resulting in hypoxia, HIF activation, and increased EPO expression that are modulated by captopril treatment. These data suggest that short-term suppression of radiation-induced EPO immediately after TBI is favorable for erythroid recovery. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596511 Mon, 13 Dec 2010 00:00:00 +0100 4596511 http://www.medworm.com/index.php?rid=4596517&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005965%2Fabstract%3Frss%3Dyes Conclusions: Our data show that purinergic signaling modulates hMSCs functions and highlights a role for extracellular nucleotides in hMSCs biology. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596517 Fri, 10 Dec 2010 00:00:00 +0100 4596517 http://www.medworm.com/index.php?rid=4596510&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005953%2Fabstract%3Frss%3Dyes Conclusions: Taken together, these results suggest a potential therapeutic use for BKT140 in multiple myeloma and leukemia patients. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596510 Mon, 06 Dec 2010 00:00:00 +0100 4596510 http://www.medworm.com/index.php?rid=4596513&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005941%2Fabstract%3Frss%3Dyes In this study, the roles of Bcl-2 family members in PRED-induced apoptosis and their prognostic value to day 8 PRED response are evaluated.Materials and Methods: Four clinically important acute lymphoblastic leukemia cell lines, three PRED-sensitive (697, Sup-B15, and RS4;11) and one PRED-resistant (REH) were studied. Thirty paired patient bone marrow samples were obtained at diagnosis (day 0) and after 7 days (day 8) of PRED monotherapy. Twenty-five patients had PRED good response and five PRED poor response. Differential expressions of Bcl-2 members were observed in those samples and BIM was further investigated using gene silencing technology in representative cell line Sup-B15.Results: The proapoptotic BH3-only Bcl-2 family member BIM was upregulated only in PRED-sensitive cells. Recei... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4596513 Fri, 03 Dec 2010 00:00:00 +0100 4596513 http://www.medworm.com/index.php?rid=4387013&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005552%2Fabstract%3Frss%3Dyes Conclusions: We succeeded to highly purify the CD34− SRCs using 18Lin mAbs and the intrabone marrow injection technique. This newly developed high-resolution purification method is indispensable to precisely characterize a distinct class of primitive human CB-derived CD34− hematopoietic stem cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387013 Mon, 29 Nov 2010 00:00:00 +0100 4387013 http://www.medworm.com/index.php?rid=4387016&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005539%2Fabstract%3Frss%3Dyes Conclusions: When started early after GVHD onset, prednisolone-related beneficial effects can affect aGVHD target organs differently, involving divergent regulation of inflammation and leukocyte migration. Specifically, a change in adhesion properties between leukocytes and endothelial cells in the gastrointestinal tract may be one of the initial steps in a cascade of steroid-related aGVHD-attenuating events. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387016 Wed, 24 Nov 2010 00:00:00 +0100 4387016 http://www.medworm.com/index.php?rid=4387008&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005540%2Fabstract%3Frss%3Dyes Conclusions: These results suggest that annexin-2 serves as an anchor for CXCL12 to help in the localization of HSCs to the niche. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387008 Wed, 24 Nov 2010 00:00:00 +0100 4387008 http://www.medworm.com/index.php?rid=4387011&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005527%2Fabstract%3Frss%3Dyes Conclusions: Overexpression of liver-specific cytokine receptors allowed the leukemic cells to invade the liver. The high concentration of leukemia stem cells in the liver suggests the cells of this leukemia are able to adapt to new extramedullar niches. The model for the investigation and development of preventative strategies against massive liver invasion are described here. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387011 Mon, 22 Nov 2010 00:00:00 +0100 4387011 http://www.medworm.com/index.php?rid=4184551&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005047%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4184551 Sat, 20 Nov 2010 06:27:16 +0100 4184551 http://www.medworm.com/index.php?rid=4387006&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005503%2Fabstract%3Frss%3Dyes Gene expression in the eukaryotic cell is regulated at a number of levels, including transcription of genomic DNA into messenger RNA (mRNA), nucleocytoplasmic export of mRNA, and translation of the exported mRNA into proteins in the cytoplasm by ribosomes. The role played by epigenetics and transcription factors associated with the control of gene expression in the developing neutrophil has been well documented and appreciated over the years. A wealth of information on the role played by transcription factors in myeloid biology has contributed to our understanding of both normal and abnormal neutrophil development. However, regulation of mRNA translation in myeloid cell maturation is much less well-studied. A better understanding of the translational control of myeloid gene expression may ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387006 Fri, 19 Nov 2010 00:00:00 +0100 4387006 http://www.medworm.com/index.php?rid=4387017&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005485%2Fabstract%3Frss%3Dyes Conclusions: Separation of ALDHbright, CD34+, or PKHbright cells enriches for AML long-term surviving capacity, which reside in the CD34+ subpopulation, as rather exclusively CD34+ cells survived and expanded in vivo and ex vivo. Long-term survival capacity may be supported by CD44v7 expression. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387017 Wed, 17 Nov 2010 00:00:00 +0100 4387017 http://www.medworm.com/index.php?rid=4387007&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005497%2Fabstract%3Frss%3Dyes Conclusions: These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387007 Wed, 17 Nov 2010 00:00:00 +0100 4387007 http://www.medworm.com/index.php?rid=4387014&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005412%2Fabstract%3Frss%3Dyes Conclusions: Human AMSCs facilitate the immunosuppressive effect of CsA on T lymphocytes through Jagged-1/Notch-related inhibition of NF-κB signaling. The combination of AMSCs and CsA represents a rationale therapeutic approach aimed to prevent adverse effects of CsA while maintaining its adequate immunosuppressive effect. Expression of Jagged-1 on AMSCs may provide an effective mechanism for the immunomodulatory activity of AMSCs via direct cell−cell interaction. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387014 Mon, 15 Nov 2010 00:00:00 +0100 4387014 http://www.medworm.com/index.php?rid=4296777&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1000545X%2Fabstract%3Frss%3Dyes This issue marks several changes in the editorial leadership of Experimental Hematology. For the last 6 years Dr. Esmail Zanjani and his editorial team have done an outstanding job of keeping our journal as a major force in a rapidly changing publication landscape. This team with the strong support of Kimberly Higgins, the Managing Editor, and our publisher Elsevier has ushered the journal into the internet era with state of art on-line submission, tracking, review and now almost immediate publication of accepted articles. Despite the emergence of several new journals and societies in the area of stem cells, hematology and cell therapy, Experimental Hematology continues to be among the top specialized journals in the fields of hematology, stem cell biology and transplantation. (Source: Exp... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296777 Fri, 12 Nov 2010 00:00:00 +0100 4296777 http://www.medworm.com/index.php?rid=4387012&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005382%2Fabstract%3Frss%3Dyes Conclusions: Each leukemia subtype possesses specific telomere dysregulations that rely on phenotype, karyotype, response to treatment, and survival. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387012 Tue, 09 Nov 2010 00:00:00 +0100 4387012 http://www.medworm.com/index.php?rid=4387010&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005394%2Fabstract%3Frss%3Dyes Conclusions: These results suggest that LR expression influenced the characteristics of leukemia cells toward an aggressive phenotype and increased the number of GM-CSFR. These changes might be partly related to enhanced GM-CSF signaling. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387010 Tue, 09 Nov 2010 00:00:00 +0100 4387010 http://www.medworm.com/index.php?rid=4296778&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005400%2Fabstract%3Frss%3Dyes The SH2-containing inositol-5′-phosphatase, SHIP (or SHIP1), is a hematopoietic-restricted phosphatidylinositide phosphatase that translocates to the plasma membrane after extracellular stimulation and hydrolyzes the phosphatidylinositol-3-kinase-generated second messenger PI-3,4,5-P3 to PI-3,4-P2. As a result, SHIP dampens down PI-3,4,5-P3-mediated signaling and represses the proliferation, differentiation, survival, activation, and migration of hematopoietic cells. There are multiple lines of evidence suggesting that SHIP may act as a tumor suppressor during leukemogenesis and lymphomagenesis. Because of its ability to skew macrophage progenitors toward M1 macrophages and naïve T cells toward T helper 1 and T helper 17 cells, SHIP may play a critical role in activating the immune syst... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296778 Tue, 09 Nov 2010 00:00:00 +0100 4296778 http://www.medworm.com/index.php?rid=4387015&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005023%2Fabstract%3Frss%3Dyes Conclusions: Based on these observations, we postulate that VSELs are the most primitive murine bone marrow−residing population of stem cells that have the potential to become specified into the hematopoietic lineage and may share some of the characteristics of long-term repopulating HSCs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387015 Thu, 28 Oct 2010 00:00:00 +0100 4387015 http://www.medworm.com/index.php?rid=4296783&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1000500X%2Fabstract%3Frss%3Dyes Conclusions: Our in vitro data suggest that the use of ZOL at appropriate doses could be explored clinically in bortezomib-resistant MM patients and combined with arsenic trioxide to increase its proapoptotic effect. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296783 Mon, 25 Oct 2010 00:00:00 +0100 4296783 http://www.medworm.com/index.php?rid=4296781&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10005011%2Fabstract%3Frss%3Dyes Conclusions: The present study suggests a new EPO-independent HSC mechanism of high-altitude erythrocytosis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296781 Mon, 25 Oct 2010 00:00:00 +0100 4296781 http://www.medworm.com/index.php?rid=4296780&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004984%2Fabstract%3Frss%3Dyes Conclusions: DNMT1 is required to maintain DNA methylation of the γ-globin gene promoter and repress γ-globin gene expression in adult-stage erythroid cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296780 Mon, 25 Oct 2010 00:00:00 +0100 4296780 http://www.medworm.com/index.php?rid=4184553&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004996%2Fabstract%3Frss%3Dyes Recently, expression patterns of microRNAs in body fluids underscored their potential as noninvasive biomarkers for various diseases. Here we summarize the current works describing microRNAs in blood cells or serum as biomarkers and the applied methodologies of small RNA purification and detection. Future challenges and the required research relating to optimization of working with microRNAs in serum will also be discussed. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4184553 Mon, 25 Oct 2010 00:00:00 +0100 4184553 http://www.medworm.com/index.php?rid=4184552&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004972%2Fabstract%3Frss%3Dyes With this issue of the Journal, my tenure as Editor-in-Chief ends. It has been a challenging and exciting 6 years. The period saw the implementation of electronic submission and processing of manuscripts, the rapid review and publication of previously reviewed and appropriately revised manuscripts, and the posting of accepted manuscripts online soon after acceptance. I want to thank Graca Almeida-Porada, Andrea Bacigalupo, Anthony Ho, Keith Humphries, Ihor Lemischka, Alan Flake, Thalia Papayannopoulou, Christopher Porada, Peter Quesenberry, Mariusz Ratajczak, Yair Reisner, Paul Simmons, Catherine Verfaillie, Mervin Yoder, and the members of the editorial board for their advice, dedication, and hard work; Kimberly Higgins was indispensible. Thanks also to our publisher Livia Berardi at Else... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4184552 Thu, 21 Oct 2010 00:00:00 +0100 4184552 http://www.medworm.com/index.php?rid=4296790&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004935%2Fabstract%3Frss%3Dyes Conclusions: The availability of this novel framework should accelerate the characterization of LICs and the translation of animal studies into clinical trials. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296790 Mon, 11 Oct 2010 00:00:00 +0100 4296790 http://www.medworm.com/index.php?rid=4296784&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004959%2Fabstract%3Frss%3Dyes Conclusions: These results suggest that the success of strategies aimed at telomerase inhibition in CML is highly dependent on the presence of functional p53 and should be explored preferentially in chronic phase CML. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296784 Mon, 11 Oct 2010 00:00:00 +0100 4296784 http://www.medworm.com/index.php?rid=4296779&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004947%2Fabstract%3Frss%3Dyes Conclusions: Together, these studies support further development of SGI-1252 for clinical use. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296779 Mon, 11 Oct 2010 00:00:00 +0100 4296779 http://www.medworm.com/index.php?rid=4387009&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004923%2Fabstract%3Frss%3Dyes Conclusions: Our results indicate that miR-146a, an LPS-induced miRNA, regulates multiple aspects of hematopoietic differentiation and survival. Furthermore, the consequences of miR-146a expression in hematopoietic cells mimics some of the reported effects with acute LPS exposure. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4387009 Thu, 07 Oct 2010 00:00:00 +0100 4387009 http://www.medworm.com/index.php?rid=4296789&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004911%2Fabstract%3Frss%3Dyes Conclusions: We demonstrate that stromal cell lines generated from embryonic microenvironments provide a tool to identify secreted proteins that play a role in the maintenance of HSC, and that at least one of the factors produced by UG26-1B6 cells responsible for preserving HSC is Wnt5a. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296789 Thu, 07 Oct 2010 00:00:00 +0100 4296789 http://www.medworm.com/index.php?rid=4296786&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004893%2Fabstract%3Frss%3Dyes Conclusions: These results further confirm that the p.P194_H195dup is a polymorphism and illustrate the difficulties that can arise in the differentiation of genetic polymorphisms from malignancy-inducing alterations. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296786 Mon, 04 Oct 2010 00:00:00 +0100 4296786 http://www.medworm.com/index.php?rid=4296787&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004881%2Fabstract%3Frss%3Dyes Conclusions: These data indicate that loss of wild-type clones at the progenitor level is a feature of MF (primary MF, post-ET MF, and post-PV MF), presumably due to expansion of the JAK2 V617F clone and that this characteristic is surprisingly independent of JAK2 V617F homozygosity, suggesting that additional genomic lesions may contribute to this unique molecular process that distinguishes MF from ET and PV. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296787 Fri, 01 Oct 2010 00:00:00 +0100 4296787 http://www.medworm.com/index.php?rid=4296788&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004145%2Fabstract%3Frss%3Dyes Conclusions: Cytokine responses are hampered in untreated CML patients, which may have an effect on various immunological processes in vivo. Interestingly, during TKI treatment, phosphorylation responses were normal, suggesting that TKI treatment does not alter the reactivity of healthy immune effector cells. However, dasatinib treatment was associated with diminished basal activation of the immunosuppressive signal transduction and activator of transcription protein 3 signaling pathway, which could have clinical significance in reversing the lymphocyte anergy against tumor cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296788 Tue, 28 Sep 2010 00:00:00 +0100 4296788 http://www.medworm.com/index.php?rid=4296782&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004868%2Fabstract%3Frss%3Dyes Conclusions: The SeV/ΔP vector would be suitable for transient expression of HoxB4 in human CD34+ cells. In addition, the SeV/ΔP vector is free of concern about transgene-related and insertional leukemogenesis and should be safer than retroviral vectors. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296782 Tue, 28 Sep 2010 00:00:00 +0100 4296782 http://www.medworm.com/index.php?rid=4090622&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004157%2Fabstract%3Frss%3Dyes Conclusions: The proof of the clonal existence of USSC is important for the assessment of biological properties unique for these unrestricted human stem cell candidates. As clones they can be subjected to advanced methods that enable defining of the multilayer nature of regulatory mechanisms through single-cell analysis. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4090622 Mon, 27 Sep 2010 23:00:00 +0100 4090622 http://www.medworm.com/index.php?rid=4296785&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004121%2Fabstract%3Frss%3Dyes Conclusions: The findings suggest that MLL fusion proteins deregulate genes critical for leukemia by excessive recruitment and impaired dissociation of EAP from target loci. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4296785 Mon, 20 Sep 2010 00:00:00 +0100 4296785 http://www.medworm.com/index.php?rid=4184566&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1000370X%2Fabstract%3Frss%3Dyes Conclusions: The kinetics of mixed chimerism as assessed by quantitative real-time polymerase chain reaction is an important prognostic predictor in the post-transplantation period of AML patients. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4184566 Mon, 20 Sep 2010 00:00:00 +0100 4184566 http://www.medworm.com/index.php?rid=4184561&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10004133%2Fabstract%3Frss%3Dyes Conclusions: Taken together, these data suggest a role for T-reg cells in limiting immune responses in CML patients and this may include immune responses to BCR-ABL. The increased frequency of T-reg cells in patients with high levels of BCR-ABL transcripts indicates that an immune mechanism may be important in the control of CML. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=4184561 Mon, 20 Sep 2010 00:00:00 +0100 4184561