MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Experimental Hematology' source. Wed, 17 Mar 2010 14:33:28 +0100 http://www.medworm.com/index.php?rid=3289414&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10000160%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289414 Sat, 20 Feb 2010 14:33:35 +0100 3289414 http://www.medworm.com/index.php?rid=3289416&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10000111%2Fabstract%3Frss%3Dyes Conclusions: Our findings underscore the importance of microenvironmental signals for lineage choice in leukemia and identify signals that are important in myeloid-erythroid lineage decisions. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289416 Thu, 21 Jan 2010 00:00:00 +0100 3289416 http://www.medworm.com/index.php?rid=3177357&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004640%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177357 Sat, 16 Jan 2010 14:22:28 +0100 3177357 http://www.medworm.com/index.php?rid=3289422&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X1000010X%2Fabstract%3Frss%3Dyes Conclusion: These studies suggest that cellular systems are more phenotypically labile than previously considered. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289422 Fri, 15 Jan 2010 00:00:00 +0100 3289422 http://www.medworm.com/index.php?rid=3289423&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X10000020%2Fabstract%3Frss%3Dyes Conclusions: The ability to generate large numbers of ECs and SMCs from a single vascular progenitor cell population is promising for therapeutic use to treat a variety of diseased and ischemic conditions. The stepwise differentiation outlined here is an efficient, reproducible method with potential for large-scale cultures suitable for clinical applications. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289423 Mon, 11 Jan 2010 00:00:00 +0100 3289423 http://www.medworm.com/index.php?rid=3289420&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004883%2Fabstract%3Frss%3Dyes Conclusion: KIR activation and HLA expression density are critical determinants for the efficacy of rituximab treatment. An explanation for the failure of rituximab treatment may be the protection of the tumor cells from ADCC by inhibiting NK-cell function with their surface HLA. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289420 Wed, 06 Jan 2010 00:00:00 +0100 3289420 http://www.medworm.com/index.php?rid=3289421&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004871%2Fabstract%3Frss%3Dyes Conclusion: We characterized, for the first time, functional radioresistant DN2-phenotype thymic T-cell precursors, the T-cell progeny of which might provide a first line of defense against infections during the lymphopenic phase post−bone marrow transplantation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289421 Mon, 04 Jan 2010 00:00:00 +0100 3289421 http://www.medworm.com/index.php?rid=3289418&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900486X%2Fabstract%3Frss%3Dyes Conclusion: These findings broaden the value of EriB as a promising candidate targeting apoptosis cascade in treatment of hematological malignancies. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289418 Mon, 04 Jan 2010 00:00:00 +0100 3289418 http://www.medworm.com/index.php?rid=3177367&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004597%2Fabstract%3Frss%3Dyes In the article entitled “Identification of small Sca-1+, Lin−, CD45− multipotential cells in the neonatal murine retina,” which appeared in the September 2009 issue of Experimental Hematology (Volume 37, Issue 9, pages 1096-1107), the correct spelling of the name of the eighth author is Volker Enzmann. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177367 Wed, 30 Dec 2009 00:00:00 +0100 3177367 http://www.medworm.com/index.php?rid=3289419&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004615%2Fabstract%3Frss%3Dyes Conclusions: We have developed a robust analytic algorithm that enables the comparison of αβ TCR repertoires between individuals and have shown that abnormal αβ TCR repertoire is a feature of a subset of patients with advanced MDS. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289419 Fri, 25 Dec 2009 00:00:00 +0100 3289419 http://www.medworm.com/index.php?rid=3289417&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004603%2Fabstract%3Frss%3Dyes Conclusion: CXCR4 inhibition to decrease cell proliferation and to control the leukemic burden may provide a novel therapeutic strategy in patients with advanced FLT3-ITD−positive AML. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289417 Fri, 25 Dec 2009 00:00:00 +0100 3289417 http://www.medworm.com/index.php?rid=3289415&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004585%2Fabstract%3Frss%3Dyes Objective: Despite a fludarabine-based treatment is the first choice of therapy in chronic lymphocytic leukemia (CLL), not all patients achieve a partial or complete response and some of them develop autoimmune manifestations. The aim of this study was to evaluate the influence of CD154 on these adverse effects because CD154 is involved in both B-cell survival and autoimmunity.Materials and Methods: Peripheral blood mononuclear cells (PBMC) from 36 patients with CLL were cultured in vitro with fludarabine or 2-chlorodeoxyadenosine for 24, 48, and 72hours.Results: Seven patients (19.4%) presented CD154 expression in PBMC cultured with purine analogues in vitro for 24 and/or 48hours, while no expression was found when cultured in media alone. These seven patients showed a decreased apoptotic... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3289415 Mon, 21 Dec 2009 00:00:00 +0100 3289415 http://www.medworm.com/index.php?rid=3080745&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004329%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080745 Sat, 12 Dec 2009 14:17:15 +0100 3080745 http://www.medworm.com/index.php?rid=3177365&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004536%2Fabstract%3Frss%3Dyes Conclusions: Our data suggest that the gene expression profiles of cells of the bone microenvironment are different in MM patients and controls, and that MSC cells, but not OBs, have a distinct transcriptional pattern associated with the occurrence of bone lesions in MM patients. These data support the idea that alterations in MSC cells may be involved in MM bone disease. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177365 Mon, 07 Dec 2009 00:00:00 +0100 3177365 http://www.medworm.com/index.php?rid=3177363&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004524%2Fabstract%3Frss%3Dyes Conclusions: MM patients carry a small subpopulation of cycling CD34+CD138+B7-H1+ plasma cells. Their presence may limit the clinical benefits of autologous CD34+ cell transplantation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177363 Mon, 30 Nov 2009 00:00:00 +0100 3177363 http://www.medworm.com/index.php?rid=3177359&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004512%2Fabstract%3Frss%3Dyes Conclusion: Gene expression analysis and BM transplantation assay demonstrated that Ang-1 maintained HSC activity in an in vitro culture. In contrast, Ang-2 reversed the effects of Ang-1/Tie2 signaling in the regulation of long-term HSCs. Our data suggest that Ang-1 is a dominant ligand for the Tie2 receptor in long HSCs in BM. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177359 Mon, 30 Nov 2009 00:00:00 +0100 3177359 http://www.medworm.com/index.php?rid=3177361&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004500%2Fabstract%3Frss%3Dyes Conclusion: Antibodies ELA85 and ELA269 were found to be useful reagents for studying the biosynthesis, processing, and trafficking of NE during normal myelopoiesis. Neutrophils from CH dogs accumulated large amounts of higher molecular weight elastase precursors compared to normal dogs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177361 Thu, 26 Nov 2009 00:00:00 +0100 3177361 http://www.medworm.com/index.php?rid=3177358&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004494%2Fabstract%3Frss%3Dyes Conclusions: Suppression of myeloid progenitor cell expansion requires signaling through TNF-RI and TNF-RII. Signals transduced through the TNF-α–TNF-RI–SIMPL pathway support hematopoietic progenitor cell survival, growth and differentiation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177358 Thu, 26 Nov 2009 00:00:00 +0100 3177358 http://www.medworm.com/index.php?rid=3177362&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004305%2Fabstract%3Frss%3Dyes Conclusions: IL-13 or IL-4 stimulation of the PMBCL cell line Karpas-1106P induced an inflammatory phenotype that resembles that of the HL cell line. Our results suggest that the autocrine release of IL-13 in HL is one critical factor that can at least partly explain the difference in phenotype between these two lymphoma entities. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177362 Fri, 20 Nov 2009 00:00:00 +0100 3177362 http://www.medworm.com/index.php?rid=3177360&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004299%2Fabstract%3Frss%3Dyes Conclusions: We show two new regulatory functions of C/EBPε in myelopoiesis: in the absence of C/EBPε, there is not only incomplete differentiation of granulocytes, but myelopoiesis is disrupted with the appearance of an intermediate cell type with monocyte and granulocyte features, and the neutrophils have abnormal chemotaxis. Restoration of expression of Hlx provides partial recovery of function; it has no effect on neutrophil maturation, but can completely ameliorate the chemotaxis defect in C/EBPε KO cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177360 Wed, 18 Nov 2009 00:00:00 +0100 3177360 http://www.medworm.com/index.php?rid=2997350&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004032%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997350 Tue, 17 Nov 2009 14:20:43 +0100 2997350 http://www.medworm.com/index.php?rid=3177366&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004287%2Fabstract%3Frss%3Dyes Conclusions: iPS cells can be generated easily from CD34+ cord blood cells through the addition of p53 inhibition to standard reprogramming conditions. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177366 Mon, 16 Nov 2009 00:00:00 +0100 3177366 http://www.medworm.com/index.php?rid=3177364&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004275%2Fabstract%3Frss%3Dyes Conclusions: These data suggest that GAS6/Mer axis regulates homing and survival of the E2A/PBX1-positive B-cell precursor ALL in the bone marrow niche. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3177364 Mon, 16 Nov 2009 00:00:00 +0100 3177364 http://www.medworm.com/index.php?rid=3080752&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004196%2Fabstract%3Frss%3Dyes Conclusion: These findings suggest that there is a low frequency of bone marrow−derived HPP-MCFC that can both self-renew at a single-cell level and differentiate toward multiple lineages of mesenchymal origin. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080752 Mon, 09 Nov 2009 00:00:00 +0100 3080752 http://www.medworm.com/index.php?rid=3080751&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004019%2Fabstract%3Frss%3Dyes Conclusion: Evaluation of CD44v-RHAMM coexpression may improve the accuracy of DLBCL prognosis and identify a subgroup of patients who will benefit from therapeutic alternatives to cyclophosphamide, doxorubicin, vincristine, and prednisone. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080751 Mon, 26 Oct 2009 00:00:00 +0100 3080751 http://www.medworm.com/index.php?rid=3080754&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003993%2Fabstract%3Frss%3Dyes Conclusion: TS-stimulated granulocyte colony-stimulating factor mobilizes high numbers of progenitors into the peripheral circulation; in turn, this blood—these progenitors—can be used upon subsequent transfusion to effectively mitigate and repair primary acute radiation injury. The transfused cells act secondarily as a bridging therapy for irradiated mice while their own immune system recovers from the radiation-induced damage. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080754 Thu, 22 Oct 2009 00:00:00 +0100 3080754 http://www.medworm.com/index.php?rid=3080746&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09004007%2Fabstract%3Frss%3Dyes Adoptive therapy with mesenchymal stem cells (MSC) is being developed as a promising approach in the treatment of graft-vs-host disease (GVHD). In vitro, MSC exhibit strong immunomodulatory properties toward alloantigen-induced T-cell responses , and although evidence from in vivo mouse models is conflicting in clinical practice, systemically delivered MSC have been proven effective in reducing GVHD . Multipotent adult progenitor cells (MAPC), a recently described population of nonhematopoietic bone-marrow−derived stem cells with a broader differentiation capacity than MSC, have currently received attention as an alternative source for immunomodulatory cell therapy. Human MAPC can be expanded for >60 population doublings, which should allow use of a single-donor cell bank to treat large... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080746 Thu, 22 Oct 2009 00:00:00 +0100 3080746 http://www.medworm.com/index.php?rid=3080753&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003956%2Fabstract%3Frss%3Dyes Conclusions: Overall, these observations show a lower rate of platelet transfusion and faster platelet recovery kinetics after RIC HSCT, but also highlight the negative effect of severe acute GVHD as a risk factor for increased need for platelet transfusions. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080753 Mon, 19 Oct 2009 00:00:00 +0100 3080753 http://www.medworm.com/index.php?rid=3080750&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003968%2Fabstract%3Frss%3Dyes Conclusions: We demonstrate an inhibitory effect of IM on TA and on the AKT/PDK pathway. Because this effect was observed in cell expressing the BCR-ABL protein as well as cells not expressing it, and in cells sensitive as well as resistant to IM, it is reasonable to assume that the inhibitory effect of IM on TA is not mediated through known IM targets. The results of this study show that cells resistant to IM with regard to its effect on BCR-ABL could still be sensitive to IM treatment regarding other cellular components. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080750 Mon, 19 Oct 2009 00:00:00 +0100 3080750 http://www.medworm.com/index.php?rid=3080749&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003944%2Fabstract%3Frss%3Dyes Conclusion: We show here that the BCR-ABL constant levels ≥0.1% (BCR-ABL plateau) in imatinib-responding patients may indicate mutation analysis. This approach highly reduces the number of examinations for mutation in chronic myeloid leukemia responders and may present cost-effective alternative applicable in clinical practice. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080749 Mon, 19 Oct 2009 00:00:00 +0100 3080749 http://www.medworm.com/index.php?rid=3080748&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900397X%2Fabstract%3Frss%3Dyes Conclusion: This cell culture model represents a useful tool to quantify hematopoietic cell transformation by defined genetic and epigenetic alterations. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080748 Mon, 19 Oct 2009 00:00:00 +0100 3080748 http://www.medworm.com/index.php?rid=3080747&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003981%2Fabstract%3Frss%3Dyes Conclusion: Our results demonstrated that this unconditioned newborn mouse model could be used for enumeration of leukemia initiating cells in ALL and should be further evaluated. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=3080747 Mon, 19 Oct 2009 00:00:00 +0100 3080747 http://www.medworm.com/index.php?rid=2900503&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003701%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900503 Sat, 17 Oct 2009 13:15:29 +0100 2900503 http://www.medworm.com/index.php?rid=2997359&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003919%2Fabstract%3Frss%3Dyes Conclusion: We demonstrated a new function of HOZOTs as helper T cells in addition to suppressor and cytotoxic activities, characterizing HOZOTs as multifunctional T cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997359 Mon, 12 Oct 2009 00:00:00 +0100 2997359 http://www.medworm.com/index.php?rid=2997356&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003907%2Fabstract%3Frss%3Dyes Objective: Animal models have provided evidence for the existence of leukemia stem cells (LSC). However, prospective isolation of human LSC from patients with acute myeloid leukemia (AML), as well as the assessment of their clinical significance, has remained a major challenge.Materials and Methods: We have studied the functional characteristics of a subset of leukemia cells that expressed CD34 and high aldehyde dehydrogenase activity (ALDHbr), which was freshly isolated from the mononuclear cells at the time of diagnosis from the marrow of 68 consecutive patients suffering from AML.Results: The percentage of ALDHbr cells ranged from 0.01% to 16.0% with a median of 0.5%. Compared to their counterparts with low aldehyde dehydrogenase activity from the same individual patients, the ALDHbr po... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997356 Mon, 12 Oct 2009 00:00:00 +0100 2997356 http://www.medworm.com/index.php?rid=2997355&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003890%2Fabstract%3Frss%3Dyes Objective: Polycythemia vera (PV) is characterized by erythrocytosis associated with the presence of the activating JAK2V617F mutation in a variable proportion of hematopoietic cells. JAK2V617F is detected in other myeloproliferative neoplasms, does not appear to be the PV-initiating event, and its specific role in deregulated erythropoiesis in PV is incompletely understood. We investigated the pathogenesis of PV to characterize abnormal proliferation and apoptosis responses and aberrant oncogenic pathway activation in primary PV erythroid precursors.Materials and Methods: Peripheral blood CD34+ cells isolated from PV patients and healthy controls were grown in liquid culture to expand a population of primary erythroblasts for experiments designed to analyze cellular proliferation, apoptos... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997355 Wed, 07 Oct 2009 00:00:00 +0100 2997355 http://www.medworm.com/index.php?rid=2997360&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003889%2Fabstract%3Frss%3Dyes Conclusions: These studies strongly suggest that human fibroblasts are derived from HSCs. In addition, the results suggest that fibrosis seen in patients with CML may be a part of the clonal process. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997360 Mon, 28 Sep 2009 00:00:00 +0100 2997360 http://www.medworm.com/index.php?rid=2997357&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003877%2Fabstract%3Frss%3Dyes Conclusions: These findings further support the ability of dasatinib to modulate the host immune response and highlights scope for off-target applications of dasatinib for the control of TNF-α–mediated inflammatory disorders. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997357 Mon, 28 Sep 2009 00:00:00 +0100 2997357 http://www.medworm.com/index.php?rid=2997354&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003683%2Fabstract%3Frss%3Dyes Conclusion: mGS cells produced multipotent hematopoietic progenitor cells with myeloid and lymphoid lineage potential in vitro and localized in the BM after intra-BM injection but, like ES cells, failed to expand or show stem cell repopulating ability in vivo. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997354 Fri, 25 Sep 2009 00:00:00 +0100 2997354 http://www.medworm.com/index.php?rid=2997358&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003658%2Fabstract%3Frss%3Dyes Conclusions: We describe a novel type of human macrophage generated in vitro after coculture with MSCs that assumes an immunophenotype defined as IL-10–high, IL-12–low, IL-6–high, and TNF-α–low secreting cells. These MSC-educated macrophages may be a unique and novel type of alternatively activated macrophage with a potentially significant role in tissue repair. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997358 Wed, 23 Sep 2009 00:00:00 +0100 2997358 http://www.medworm.com/index.php?rid=2997353&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900366X%2Fabstract%3Frss%3Dyes Aplastic anemia (AA) is characterized by a reduced number of hematopoietic stem cells and fatty replacement in the bone marrow. Transcriptional factor GATA-2 plays several important roles in both hematopoiesis and adipogenesis. Decreased levels of GATA-2 compromise the proliferation and survival of hematopoietic stem cells. GATA-2 suppresses adipocyte differentiation through direct inhibition of adipogenic factors, including peroxisome proliferator-activated receptor−γ (PPARγ). Previous studies have shown that expression of GATA-2 is decreased in marrow CD34-positive cells in AA. To elucidate the mechanisms of fatty marrow replacement, we evaluated the mRNA expression for GATA-2 and PPARγ in mesenchymal stem cells (MSCs) from patients with AA by quantitative real-time polymerase chain... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997353 Wed, 23 Sep 2009 00:00:00 +0100 2997353 http://www.medworm.com/index.php?rid=2997352&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003646%2Fabstract%3Frss%3Dyes Conclusion: Our results show that the hCD68 promoter directs gene expression in a macrophage population distinct from that defined by classical monocyte-macrophage markers or promoters. Whether this population is functionally distinct remains to be established. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997352 Wed, 23 Sep 2009 00:00:00 +0100 2997352 http://www.medworm.com/index.php?rid=2997351&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003671%2Fabstract%3Frss%3Dyes In this study, we generated a zebrafish ortholog of human JAK2V617F (referred herewith jak2aV581F) by site-directed mutagenesis and examined its relevance as a model of human PV.Materials and Methods: Zebrafish embryos at one-cell stage were injected with jak2aV581F mRNA (200pg/embryo). In some experiments, the embryos were treated with a specific JAK2 inhibitor, TG101209. The effects of jak2a stimulation on hematopoiesis, jak/stat signaling, and erythropoietin signaling were evaluated at 18-somites.Results: Injection with jak2aV581F mRNA significantly increased erythropoiesis, as enumerated by flow cytometry based on gfp+ population in dissociated Tg(gata1:gfp) embryos. The response was reduced by stat5.1 morpholino coinjection (control: 4.37% ± 0.08%; jak2aV581F injected: 5.71% ± 0.07%... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2997351 Wed, 23 Sep 2009 00:00:00 +0100 2997351 http://www.medworm.com/index.php?rid=2805783&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003348%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805783 Fri, 18 Sep 2009 16:36:00 +0100 2805783 http://www.medworm.com/index.php?rid=2900509&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003610%2Fabstract%3Frss%3Dyes Conclusions: These results of our present study suggested an accelerated cellular senescence occurred in MDS, and the cellular senescence may be involved in the progression and prognosis of MDS. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900509 Sun, 13 Sep 2009 23:00:00 +0100 2900509 http://www.medworm.com/index.php?rid=2900514&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003609%2Fabstract%3Frss%3Dyes Conclusion: This is the first time that a small-molecule c-MPL agonist has been demonstrated to promote net expansion of HSCs. NR-101 is more efficient in ex vivo expansion of HSCs than TPO. NR-101 could be a useful tool for the therapeutic manipulation of human HSCs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900514 Tue, 08 Sep 2009 23:00:00 +0100 2900514 http://www.medworm.com/index.php?rid=2900513&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003592%2Fabstract%3Frss%3Dyes Conclusions: These findings suggest that platelet WASP deficiency results in an increase in platelet clearance rates by two mechanisms: an antibody-independent mechanism that largely requires WASP deficiency in trans, and an antibody-dependent mechanism that does not. Both an increased incidence of antiplatelet antibodies and an increased susceptibility to their effects contribute to antibody-dependent clearance of WASP(−) platelets. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900513 Thu, 03 Sep 2009 23:00:00 +0100 2900513 http://www.medworm.com/index.php?rid=2900508&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003300%2Fabstract%3Frss%3Dyes Conclusion: Thus, Trk receptors represent a novel therapeutic target for the treatment of NHL. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900508 Mon, 31 Aug 2009 23:00:00 +0100 2900508 http://www.medworm.com/index.php?rid=2900504&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003312%2Fabstract%3Frss%3Dyes Conclusions: We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900504 Mon, 31 Aug 2009 23:00:00 +0100 2900504 http://www.medworm.com/index.php?rid=2900512&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003282%2Fabstract%3Frss%3Dyes Conclusion: NIC greatly increases Mk ploidy under a wide range of conditions without altering Mk morphology. Inhibition of SIRT1 and/or SIRT2 is primarily responsible for NIC effects on Mk maturation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900512 Wed, 26 Aug 2009 23:00:00 +0100 2900512 http://www.medworm.com/index.php?rid=2900510&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003294%2Fabstract%3Frss%3Dyes Conclusions: Marmoset immune system was basically similar to human and mouse systems. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900510 Wed, 26 Aug 2009 23:00:00 +0100 2900510 http://www.medworm.com/index.php?rid=2900507&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003269%2Fabstract%3Frss%3Dyes Conclusions: Pim-1 is variably expressed in leukemia cell lines and associated with drug resistance. Targeting Pim-1 with monoclonal antibody could be explored for the treatment of leukemia and may represent a novel strategy to overcome drug resistance. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900507 Mon, 24 Aug 2009 23:00:00 +0100 2900507 http://www.medworm.com/index.php?rid=2900506&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003270%2Fabstract%3Frss%3Dyes Conclusion: These results provide the basis for the further study of small molecule SHIP activators to improve MM patient outcomes. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900506 Mon, 24 Aug 2009 23:00:00 +0100 2900506 http://www.medworm.com/index.php?rid=2693388&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002902%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693388 Thu, 13 Aug 2009 10:57:23 +0100 2693388 http://www.medworm.com/index.php?rid=2900511&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003130%2Fabstract%3Frss%3Dyes Conclusion: In conclusion, we have developed a serum-free megakaryocyte induction medium, and the combination of serum-free megakaryocyte and serum-free hematopoietic stem cell media can generate a large amount of functional megakaryocytes efficiently. Our method represents a promising source of megakaryocytes and platelets for future cell therapy. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900511 Wed, 05 Aug 2009 23:00:00 +0100 2900511 http://www.medworm.com/index.php?rid=2900505&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003129%2Fabstract%3Frss%3Dyes Conclusions: Fludarabine and procarbazine have a dramatic influence, especially on the most immature hematopoietic cells, mirrored in reduced numbers of LTC-IC. This finding is consistent with clinical observations (poor mobilization after fludarabine) and offers an insight into the mechanism of fludarabine-induced myelotoxicity. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2900505 Sun, 02 Aug 2009 23:00:00 +0100 2900505 http://www.medworm.com/index.php?rid=2805791&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09003105%2Fabstract%3Frss%3Dyes Conclusion: Taken together, these results suggest that specific genetic signatures might be responsible for differential DC differentiation and maturation between APB vs CB, and may provide insight into molecular mechanisms regulating differential immune responses between neonates and adults. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805791 Sun, 02 Aug 2009 23:00:00 +0100 2805791 http://www.medworm.com/index.php?rid=2805795&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002720%2Fabstract%3Frss%3Dyes Conclusion: Cotransplantation with MSCs would improve the efficacy of transplantation of gene-modified HSCs in primates, with enhanced engraftment in BM as well as increased chimerism in peripheral blood through migration and homing. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805795 Sun, 26 Jul 2009 23:00:00 +0100 2805795 http://www.medworm.com/index.php?rid=2805792&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002732%2Fabstract%3Frss%3Dyes We reported the ability to ex vivo expand (EvE) CB mononuclear cells (MNC) after short-term incubation with anti-CD3, interleukin (IL)-2, IL-7, and IL-12 (antibody/cytokine [AB/CY]) into subpopulations of CD3−/56+ natural killer (NK) cells with enhanced in vitro and in vivo tumor cytotoxicity.Materials and Methods: We compared 2- vs 7-day EvE of rethawed CB MNCs in AB/CY and activation of NK and NK-like T (NKT) cell (CD3+/56+) subsets expressing specific NK-cell receptors along with IL-15, IL-18, and interferon-γ production.Results: Nonadherent total cell number were significantly increased at day 7 (p (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805792 Sun, 26 Jul 2009 23:00:00 +0100 2805792 http://www.medworm.com/index.php?rid=2693392&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002586%2Fabstract%3Frss%3Dyes Conclusions: These results demonstrate that the nonpeptidyl TPOR agonist eltrombopag stimulates platelet signal transduction with little or no effect on overall platelet function, in contrast to TPO, which significantly primes platelet activation. These data demonstrate that effects of TPOR ligands on platelet function can vary depending on the specific mechanism utilized to stimulate the TPOR. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693392 Thu, 23 Jul 2009 23:00:00 +0100 2693392 http://www.medworm.com/index.php?rid=2805794&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002574%2Fabstract%3Frss%3Dyes Conclusion: This report demonstrates a critical role of MEK-ERK1/2 pathway in MK differentiation, motility, and proplatelet formation. This study highlights several differences between BM- and FL-derived MKs, which are discussed. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805794 Mon, 20 Jul 2009 23:00:00 +0100 2805794 http://www.medworm.com/index.php?rid=2805789&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002562%2Fabstract%3Frss%3Dyes In this study, histopathological classification of 490 MPN cases was correlated with the allelic burden of JAK2V617F and MPLW515L.Results: Ph− MPN entities largely overlap with regard to JAK2V617F and MPLW515L allele burden, but ET displayed mutant allele burden 40% JAK2V617F alleles (median, 24% JAK2V617F alleles; n=90; p (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805789 Mon, 20 Jul 2009 23:00:00 +0100 2805789 http://www.medworm.com/index.php?rid=2805786&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002550%2Fabstract%3Frss%3Dyes Conclusions: The engraftment and expansion of these leukemia cells in dogs will allow studies into the biology of leukemia and development and evaluation of novel antileukemia therapies in a clinically relevant large animal model. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805786 Mon, 20 Jul 2009 23:00:00 +0100 2805786 http://www.medworm.com/index.php?rid=2805790&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002537%2Fabstract%3Frss%3Dyes Conclusion: This study indicates that MPN hematopoietic progenitor cells do not necessarily always acquire genetic events in the same sequence. (Chromosomally abnormal progenitor cells are closely associated with JAK2V617F homozygosity; p=0.0001.). Chromosomal abnormalities such as +8, +9 can occasionally precede acquisition of JAK2V617F. These findings support the existence of earlier genetic events that precede JAK2V617F or cytogenetic abnormalities in MPN hematopoietic progenitor cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805790 Thu, 16 Jul 2009 23:00:00 +0100 2805790 http://www.medworm.com/index.php?rid=2603904&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002215%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603904 Thu, 16 Jul 2009 10:59:58 +0100 2603904 http://www.medworm.com/index.php?rid=2805788&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002549%2Fabstract%3Frss%3Dyes Conclusion: We demonstrated that WEB-2170 is a powerful antileukemic agent with interesting translational opportunities to treat AML and described mechanisms of drug-induced intrinsic and extrinsic apoptosis both in AML cell lines and blasts from AML patients by addressing PTEN as the master regulator of the whole process. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805788 Wed, 15 Jul 2009 23:00:00 +0100 2805788 http://www.medworm.com/index.php?rid=2805785&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002525%2Fabstract%3Frss%3Dyes Conclusion: Our results indicate that remodeling of the upstream elements may be the primary event in activation of α-globin gene expression. Activation of α-globin genes upon EPO treatment involves initial binding of Pol II, downregulation of pre-existing factors like NF-E2, removal of CTCF from the locus, then rebinding of CTCF in an altered pattern, and concurrent or subsequent binding of transcription factors like GATA-1. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805785 Tue, 14 Jul 2009 23:00:00 +0100 2805785 http://www.medworm.com/index.php?rid=2805787&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002513%2Fabstract%3Frss%3Dyes Conclusion: Besides expanding knowledge of the molecular basis of the NK/DC cross-talk, our study demonstrates that NK cells from NK-LDGL patients are impaired in their ability to interact with Mo-DC. The possible relationship between such abnormal NK cell/DC interactions and chronic NK cell proliferation are discussed. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805787 Sun, 05 Jul 2009 23:00:00 +0100 2805787 http://www.medworm.com/index.php?rid=2805793&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002501%2Fabstract%3Frss%3Dyes Conclusion: These results demonstrate an arrangement by which NO and fetal hemoglobin inducers may stimulate globin genes in erythroid cells via the common paracrine effect of bone marrow stromal cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805793 Thu, 02 Jul 2009 23:00:00 +0100 2805793 http://www.medworm.com/index.php?rid=2805784&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002495%2Fabstract%3Frss%3Dyes Conclusion: The mechanism responsible for increased γ-globin expression in cultured EPC was unexpectedly not associated with increased DNA hypomethylation of the γ-globin gene promoter compared to normal BM erythroid cells, in contrast to BM erythroid cells of decitabine-treated baboons. Rather, increased fetal hemoglobin in EPC cultures was associated with a fetal Iγ/Vγ chain ratio and a difference in the size of the BCL11A protein compared to normal BM erythroid cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2805784 Thu, 02 Jul 2009 23:00:00 +0100 2805784 http://www.medworm.com/index.php?rid=2693399&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002483%2Fabstract%3Frss%3Dyes Conclusion: Together, these studies clarify the current controversy regarding the ability of HSCs to give rise to adipocytes. Furthermore, our primary culture method that generates adipocytes from uncommitted hematopoietic cells should contribute to the studies of the mechanisms of early adipocytic differentiation and may lead to development of therapeutic solutions for many general obesity issues. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693399 Thu, 02 Jul 2009 23:00:00 +0100 2693399 http://www.medworm.com/index.php?rid=2490517&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001830%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490517 Thu, 25 Jun 2009 07:44:03 +0100 2490517 http://www.medworm.com/index.php?rid=2693390&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002197%2Fabstract%3Frss%3Dyes Conclusion: These findings support the concept of a continuum between ET JAK2 mutated and PV, not only in reference to the hematological phenotype, but also in terms of vascular events. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693390 Wed, 24 Jun 2009 23:00:00 +0100 2693390 http://www.medworm.com/index.php?rid=2693400&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002124%2Fabstract%3Frss%3Dyes Conclusions: Findings of this study provide the proof of concept that C48—a novel, single, bifunctional therapeutic modality enabled successful allogeneic, unmanipulated bone marrow transplantation without GVHD, and with lasting specific tolerance. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693400 Wed, 17 Jun 2009 23:00:00 +0100 2693400 http://www.medworm.com/index.php?rid=2693398&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002161%2Fabstract%3Frss%3Dyes Conclusions: The neonatal retina is an abundant postnatal source of multipotential VSEL-like cells that can differentiate in culture into a variety of lineages. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693398 Wed, 17 Jun 2009 23:00:00 +0100 2693398 http://www.medworm.com/index.php?rid=2693396&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900215X%2Fabstract%3Frss%3Dyes Conclusion: This study demonstrates that GAG mimetics induce efficient mobilization of HSPCs, associated with an activation of pro−MMP-9 and a modification in the SDF-1 concentration gradient between bone marrow and peripheral blood. We suggest that structural features of GAGs can modify the nature of mobilized cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693396 Wed, 17 Jun 2009 23:00:00 +0100 2693396 http://www.medworm.com/index.php?rid=2693395&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002112%2Fabstract%3Frss%3Dyes Conclusion: The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693395 Wed, 17 Jun 2009 23:00:00 +0100 2693395 http://www.medworm.com/index.php?rid=2693394&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002136%2Fabstract%3Frss%3Dyes Objective: Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.Materials and Methods: Circulating EPC counts (KDR+/CD34+/Cd45dim cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interleukin-8 were investigated in SCD patients during asymptomatic state (n=66) and painful crisis (n=36) and compared to healthy controls (n=13).Results: EPC counts were comparable between controls (0; range, 0–1.1 cells/mL) and patients (0; range, 0–0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0–186 cells/mL; p (Sourc... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693394 Wed, 17 Jun 2009 23:00:00 +0100 2693394 http://www.medworm.com/index.php?rid=2693391&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002148%2Fabstract%3Frss%3Dyes Conclusion: Thus, fungal colonization may not predict susceptibility to infection in the presence of these single nucleotide polymorphisms. The finding that defective viral but not fungal sensing may predict susceptibility to infection highlights the divergent function of TLRs in the pathogenesis of opportunistic infections. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693391 Wed, 17 Jun 2009 23:00:00 +0100 2693391 http://www.medworm.com/index.php?rid=2603906&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002100%2Fabstract%3Frss%3Dyes This letter aims to make cell culture investigators using human or animal long-term cell cultures aware of potential contamination by nanobacteria-like particles , recently proposed as nanons . (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603906 Sun, 14 Jun 2009 23:00:00 +0100 2603906 http://www.medworm.com/index.php?rid=2693397&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09002094%2Fabstract%3Frss%3Dyes Conclusions: Our studies indicate there are interactions between intrinsic factors influenced by epigenetic mechanisms and external environmental signals in the regulation of HSC expansion. Epigenetic influences on HSC can be accentuated by environmental factors. Regulation of the rate of divisions may be a critical determinant for the maintenance of HSC functional potency during ex vivo expansion. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693397 Sun, 07 Jun 2009 23:00:00 +0100 2693397 http://www.medworm.com/index.php?rid=2693393&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001817%2Fabstract%3Frss%3Dyes Conclusion: These results suggest that the absence of VE in G4N and G6N EBs does not prevent emergence of definitive progenitors from EBs. However, the decreased level of VEGF and Indian hedgehog production in VE devoid G4N and G6N EBs attenuates definitive hematopoietic progenitor cell expansion. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693393 Thu, 04 Jun 2009 23:00:00 +0100 2693393 http://www.medworm.com/index.php?rid=2693389&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001805%2Fabstract%3Frss%3Dyes Although the concept of a humoral regulator of erythropoiesis was introduced more than 100 years ago, its existence was first firmly established a little more than 50 years ago. This review briefly describes the historical development of information about erythropoietin. It then describes our current understanding of where erythropoietin is produced; the factors that regulate its rate of production; how erythropoietin acts at the cellular level to stimulate erythropoiesis; and its role in the regulation of the rate of erythropoiesis. Finally, it discusses the clinical uses of erythropoietin in the diagnosis and therapy of hematopoietic diseases. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2693389 Wed, 03 Jun 2009 23:00:00 +0100 2693389 http://www.medworm.com/index.php?rid=2603914&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001799%2Fabstract%3Frss%3Dyes Conclusion: Long-term expression in vivo and inducible secretion of hsTNFR1 in murine hematopoietic cells support the potential use of storage organelles in hematopoietic cells as vehicles for targeting inflamed/malignant sites with therapeutically active agents. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603914 Sun, 31 May 2009 23:00:00 +0100 2603914 http://www.medworm.com/index.php?rid=2603916&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001787%2Fabstract%3Frss%3Dyes This study was designed to investigate the dynamics of transmigration and engraftment of hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) introduced via intra−bone marrow transplantation (IBMT), which is reserved as a novel strategy for possible clinical transplantation.Materials and Methods: The early distribution pattern and engraftment level of human HS/PCs introduced via traditional intravenous transplantation (IVT) and IBMT routes were compared in the xenotransplanted nonobese diabetic/severe combined immunodeficient mouse model by means of flow cytometric analysis and an optical imaging system.Results: It was obvious that a good deal of IVT-introduced donor cells were entrapped in the liver and lung, 0.06% ± 0.01% and 0.07% ± 0.02%, respectively. Meanw... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603916 Wed, 27 May 2009 23:00:00 +0100 2603916 http://www.medworm.com/index.php?rid=2603915&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001763%2Fabstract%3Frss%3Dyes Conclusion: In this study, we have looked at the site-specific phosphorylation in the wild-type Flt3 in comparison to the mutants found in AML. We observed not only quantitative changes but, more importantly, qualitative differences in the phosphorylation patterns of the wild-type and the mutated Flt3 receptors, which might enhance the understanding of the mechanisms by which Flt3 contributes to AML in patients with mutations in Flt3. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603915 Wed, 27 May 2009 23:00:00 +0100 2603915 http://www.medworm.com/index.php?rid=2603911&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001751%2Fabstract%3Frss%3Dyes Conclusion: SNP-chip analysis detected in MCL very small genomic gains/losses, as well as aUPDs, which could not be detected by more conventional methods. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603911 Wed, 27 May 2009 23:00:00 +0100 2603911 http://www.medworm.com/index.php?rid=2603910&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001775%2Fabstract%3Frss%3Dyes Conclusions: MEDII treatment of hESCs and hiPSCs alike selectively enhances their differentiation into mesodermal cells and allows subsequent generation of detectable levels of hematopoietic progenitors with in vitro and in vivo differentiating activity. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603910 Wed, 27 May 2009 23:00:00 +0100 2603910 http://www.medworm.com/index.php?rid=2490530&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001398%2Fabstract%3Frss%3Dyes A prominent Russian scientist and leading specialist in experimental hematology, Professor Joseph Lvovich Chertkov, passed away on March 20, 2009, at the age of 81. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490530 Sun, 24 May 2009 23:00:00 +0100 2490530 http://www.medworm.com/index.php?rid=2429655&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001428%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2429655 Sat, 23 May 2009 04:51:02 +0100 2429655 http://www.medworm.com/index.php?rid=2603908&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900174X%2Fabstract%3Frss%3Dyes Conclusions: Functional studies presented here demonstrate a direct relationship between transient knockdown of MASTL kinase gene expression and reduction of circulating thrombocytes in zebrafish. This transient knockdown of MASTL in zebrafish correlates with a decrease in the expression of the thrombopoietin receptor, c-mpl, and the CD41 platelet adhesion protein, GpIIb, but has no effect on essential housekeeping zebrafish gene, EF1α. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603908 Tue, 19 May 2009 23:00:00 +0100 2603908 http://www.medworm.com/index.php?rid=2603909&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001593%2Fabstract%3Frss%3Dyes Conclusions: These results support the notion that EML cell line has the capacity for T-cell differentiation. Remarkably, induction of T-lineage gene expression and differentiation of EML cells into distinct stages of T-cell development were very similar to previously described T-cell differentiation of adult hematopoietic stem cells and progenitors in OP9-DL1 cocultures. Thus, EML/OP9-DL1 coculture could be a useful experimental system to study the role of particular genes in T-cell lineage specification, commitment, and differentiation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603909 Sun, 17 May 2009 23:00:00 +0100 2603909 http://www.medworm.com/index.php?rid=2603907&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900160X%2Fabstract%3Frss%3Dyes Conclusion: These data provide evidence that GATA-1 can reverse STAT3-mediated γ-globin gene silencing in erythroid cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603907 Sun, 17 May 2009 23:00:00 +0100 2603907 http://www.medworm.com/index.php?rid=2490518&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001581%2Fabstract%3Frss%3Dyes Long-term engrafting marrow hematopoietic stem cells have been considered to be a quiescent stem cell in G0. However, there are contradictory reports on this point in the literature, showing marked variability of results over time and between mice. Furthermore, there are circadian rhythms for stem cells and progenitors. In general, most studies have not taken stochastic variability or circadian rhythms into account. In addition, stem cell purification has represented the present gold standard in stem cell research. However, evidence exists that the stem cell separations leave behind most stem cells and are not random. Thus, purified stem cells may not be representative of the stem cells in the unseparated marrow cell population. The epitope-based purification of stem cells may have misled ... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490518 Sun, 17 May 2009 23:00:00 +0100 2490518 http://www.medworm.com/index.php?rid=2603917&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X0900157X%2Fabstract%3Frss%3Dyes Conclusions: We conclude that T-cell depletion with CD6 antibody and complement induces graft-vs-host tolerance without jeopardizing engraftment. DLT on days 3, 7, and 14 after transplantation produced GVHD, but it failed to abrogate tolerance in two of four dogs transfused on day 20. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603917 Wed, 13 May 2009 23:00:00 +0100 2603917 http://www.medworm.com/index.php?rid=2603913&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001386%2Fabstract%3Frss%3Dyes Conclusion: Administration of zoledronate–activated Vγ9γδ T LAK cells is a safe and promising immunotherapy approach for treatment of patients with multiple myeloma. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603913 Thu, 30 Apr 2009 23:00:00 +0100 2603913 http://www.medworm.com/index.php?rid=2603912&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001374%2Fabstract%3Frss%3Dyes Conclusion: We confirm that in MDS patients the TCR-βV repertoire is overall extremely contracted, especially in cytotoxic T cells. This pattern is mainly determined by selective proliferations of both helper and cytotoxic T cells, which are, however, mostly polyclonal in the former and oligoclonal in the latter. Such a difference, possibly related to the different human leukocyte antigen restriction, could reflect the selective involvement of cytotoxic T cells either in the anti-tumor immune surveillance or in an autoreactive aggression toward hematopoietic precursors. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603912 Thu, 30 Apr 2009 23:00:00 +0100 2603912 http://www.medworm.com/index.php?rid=2490528&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001350%2Fabstract%3Frss%3Dyes Conclusion: Reciprocal expression of Bmi1 and Mel-18 regulated HSC function. Here, we observed that expression of the PcG genes—bmi1 and mel-18—is correlated with self-renewal and differentiation of HSCs. Thus, it was suggested that the balance between Bmi1 and Mel-18 regulates self-renewal of HSCs. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490528 Thu, 30 Apr 2009 23:00:00 +0100 2490528 http://www.medworm.com/index.php?rid=2490524&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001362%2Fabstract%3Frss%3Dyes Conclusion: These results indicate that STYK1/NOK mRNA is widely expressed in the patients with acute leukemia and suggest that inhibition of this molecule could potentially serve as a novel therapeutic target. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490524 Thu, 30 Apr 2009 23:00:00 +0100 2490524 http://www.medworm.com/index.php?rid=2490521&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001325%2Fabstract%3Frss%3Dyes Conclusion: This study reveals complex patterns of histone modifications associated with highly expressed, moderately expressed, and unexpressed genes. Activation of γ postnatally will likely require extensive modification of the histones in a large domain around the γ genes. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490521 Tue, 28 Apr 2009 23:00:00 +0100 2490521 http://www.medworm.com/index.php?rid=2603905&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001349%2Fabstract%3Frss%3Dyes Conclusion: Runt-related transcription factor 2 and Wnt pathways could be therapeutic targets in the treatment of multiple myeloma bone disease to counterbalance the block of osteogenic differentiation induced by multiple myeloma cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2603905 Sun, 26 Apr 2009 23:00:00 +0100 2603905 http://www.medworm.com/index.php?rid=2490522&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001337%2Fabstract%3Frss%3Dyes Conclusion: This is one of the first reports to show that changes in DNA methylation patterns across large domains around non-CpG island genes correspond with changes in developmentally regulated histone modifications and gene expression. These data support a new model in which extended domains of DNA hypomethylation and active histone marks are coordinately established to achieve developmentally specific gene expression of non-CpG island genes. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490522 Sun, 26 Apr 2009 23:00:00 +0100 2490522 http://www.medworm.com/index.php?rid=2490526&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001313%2Fabstract%3Frss%3Dyes Objective: The purpose of this study was to examine the antitumor immune function of γδ T cells and to scan the granzyme B gene for the known single nucleotide polymorphism in breast cancer patients and normal controls.Materials and Methods: Levels, cytotoxicity, and functional capacity of γδ T cells in peripheral blood mononuclear cells were assessed by flow cytometry, 51Cr release, and ELISpot assays, respectively. Furthermore, sequence based typing was adopted to screen for granzyme B gene polymorphism.Results: We have found that the frequency and function of γδ T cells are reduced both in peripheral blood mononuclear cells of 30 newly diagnosed breast cancer patients (2 [1.2, 3]), compared with 38 normal controls (3.2 [2.5, 5.7]) (p=0.02). In addition, resting γδ T cells from b... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490526 Tue, 21 Apr 2009 23:00:00 +0100 2490526 http://www.medworm.com/index.php?rid=2350723&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000526%2Fabstract%3Frss%3Dyes Conclusion: Serpina1 protein in the BM extracellular fluid is predominantly produced by OB. This indicates that Serpina1 may play a regulatory role in the maintenance of HSC in the OB stem cell niche. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350723 Tue, 21 Apr 2009 09:13:02 +0100 2350723 http://www.medworm.com/index.php?rid=2350722&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000514%2Fabstract%3Frss%3Dyes Conclusion: These results showed that UC-MSCs had higher endothelial differentiation potential than BM-MSCs. Therefore, UC-MSCs are more favorable choice than BM-MSCs for neovascularization of engineered tissues. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350722 Tue, 21 Apr 2009 09:12:53 +0100 2350722 http://www.medworm.com/index.php?rid=2350721&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000319%2Fabstract%3Frss%3Dyes Conclusions: The CIK phenotype, that is CD45RA+, CCR7−, CD62L−weakly positive, CD11a+, CD27+, CD28−, macrophage inflammatory protein 1α+, perforin+, Fas ligand+ coincides almost exactly with that described for the T RA+ effector memory CD27 single positive subset of terminally differentiated human memory T cells. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350721 Tue, 21 Apr 2009 09:12:51 +0100 2350721 http://www.medworm.com/index.php?rid=2350720&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000095%2Fabstract%3Frss%3Dyes Conclusion: Results indicate that humoral factor(s) released by MSCs exert a suppressive effect on the B-cell terminal differentiation. Suppression may be mediated through inhibition of B-lymphocyte–induced maturation protein–1 expression, but the nature of the factor(s) is yet to be determined. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350720 Tue, 21 Apr 2009 09:12:40 +0100 2350720 http://www.medworm.com/index.php?rid=2350719&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000113%2Fabstract%3Frss%3Dyes Conclusions: AS101 in combination with a subtherapeutic dose of melphalan had increased beneficial effect relative to each agent alone in a mouse MM model. In addition, AS101 might be useful for targeting interactions between myeloma cells and the bone marrow microenvironment. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350719 Tue, 21 Apr 2009 09:12:38 +0100 2350719 http://www.medworm.com/index.php?rid=2350718&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000307%2Fabstract%3Frss%3Dyes Conclusion: Although how leukemic cells overcome the antiproliferative effects of Lnk is not yet clear, our data highlight the multifaceted role negative feedback mechanisms play in malignant transformation. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350718 Tue, 21 Apr 2009 09:12:33 +0100 2350718 http://www.medworm.com/index.php?rid=2350717&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000101%2Fabstract%3Frss%3Dyes Conclusion: These results imply that integral regulation of erythropoiesis is operated by low O2 concentrations, beyond the EPO/EPO-responsive cells loop and provide a tool to optimize the technology for ex vivo production of RBC. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350717 Tue, 21 Apr 2009 09:12:29 +0100 2350717 http://www.medworm.com/index.php?rid=2350716&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000538%2Fabstract%3Frss%3Dyes Conclusion: These data suggest that glucocorticoids inhibit erythroid maturation not only through a transcriptional mechanism, but also through a rapid membrane-associated pathway that interferes with EPO receptor signaling. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350716 Tue, 21 Apr 2009 09:12:27 +0100 2350716 http://www.medworm.com/index.php?rid=2350715&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000083%2Fabstract%3Frss%3Dyes Conclusions: Our data indicate that the BMP-4−dependent stress erythropoiesis pathway regulates extramedullary stress erythropoiesis, which occurs primarily in the murine spleen or in the case of splenectomized mice, in the adult liver. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350715 Tue, 21 Apr 2009 09:12:23 +0100 2350715 http://www.medworm.com/index.php?rid=2350714&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000502%2Fabstract%3Frss%3Dyes Conclusion: This study identifies GAR22 as a novel and direct TR target gene. Our results suggest that hormone-induced GAR22 might represent an important trigger of growth inhibition induced by thyroid hormone in red cell progenitors. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350714 Tue, 21 Apr 2009 09:12:08 +0100 2350714 http://www.medworm.com/index.php?rid=2350713&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000496%2Fabstract%3Frss%3Dyes Conclusion: We identified a significant role for the A2bAR in the regulation of TNF-α, which would contribute to the anti-inflammatory actions of adenosine under vascular stress. This conclusion could focus attention on this receptor as a therapeutic target. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350713 Tue, 21 Apr 2009 09:11:41 +0100 2350713 http://www.medworm.com/index.php?rid=2350712&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000939%2Fabstract%3Frss%3Dyes (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2350712 Tue, 21 Apr 2009 09:11:39 +0100 2350712 http://www.medworm.com/index.php?rid=2490525&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001283%2Fabstract%3Frss%3Dyes Conclusion: Mutations of the PSMB5 gene resulting in substitutions of Ala49 and Ala50 of PSMB5 protein can confer varying bortezomib resistance. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490525 Thu, 16 Apr 2009 23:00:00 +0100 2490525 http://www.medworm.com/index.php?rid=2490519&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001295%2Fabstract%3Frss%3Dyes Conclusions: Proteomic analysis should be further explored to define factors important in predicting clinical response in patients with acute myeloid leukemia. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490519 Thu, 16 Apr 2009 23:00:00 +0100 2490519 http://www.medworm.com/index.php?rid=2490527&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001155%2Fabstract%3Frss%3Dyes Conclusions: TIMPs are synthesized and organized in MKs and platelets independently of other secreted proteins present in α-granule pools. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490527 Sun, 05 Apr 2009 23:00:00 +0100 2490527 http://www.medworm.com/index.php?rid=2490520&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09001167%2Fabstract%3Frss%3Dyes Conclusions: Our finding demonstrates the clinical relevance of posttransplantation therapies to upgrade remission, and of remission's depth for long-term survival in myeloma patients. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490520 Sun, 05 Apr 2009 23:00:00 +0100 2490520 http://www.medworm.com/index.php?rid=2490529&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000915%2Fabstract%3Frss%3Dyes Conclusion: In summary, our data suggest that induction of Notch signaling enhances the osteogenic differentiation of hBMSCs while inhibiting the adipogenic fate. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490529 Fri, 27 Mar 2009 00:00:00 +0100 2490529 http://www.medworm.com/index.php?rid=2490523&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.exphem.org%2Farticle%2FPIIS0301472X09000903%2Fabstract%3Frss%3Dyes Conclusions: Ex vivo culture of lin− cells for γ-retroviral transduction downregulates CXCR4 expression and markedly impairs homing and engraftment of murine lin− marrow in submyeloablated hosts. While inhibition of CD26 activity with DipA increases homing and engraftment of fresh lin− cells, DipA treatment does not improve homing and engraftment of cultured lin− marrow cells in submyeloablated congenic hosts. (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2490523 Fri, 27 Mar 2009 00:00:00 +0100 2490523 http://www.medworm.com/index.php?rid=2288607&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302917%26dopt%3DAbstract CONCLUSION: GPI-anchored protein deficiency in Burkitt lymphoma cells is not due to a genetic mutation (e.g., PIGA); rather, the lack of GPI-anchored proteins results from transcriptional silencing of PIGL and PIGY. PMID: 19302917 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288607 Wed, 25 Mar 2009 23:04:34 +0100 2288607 http://www.medworm.com/index.php?rid=2288606&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302918%26dopt%3DAbstract CONCLUSION: Based on this observation, distinct self-renewal regulatory mechanisms in LSCs may be considered as one of the causes of worsening of the features of leukemia after injury and relapse. PMID: 19302918 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288606 Wed, 25 Mar 2009 23:04:25 +0100 2288606 http://www.medworm.com/index.php?rid=2288605&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302919%26dopt%3DAbstract CONCLUSION: Combined inhibition of ILK and FLT-3 with a small molecule kinase inhibitor can achieve selective targeting of AML rather than normal hematopoietic progenitors. PMID: 19302919 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288605 Wed, 25 Mar 2009 23:04:17 +0100 2288605 http://www.medworm.com/index.php?rid=2288604&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302920%26dopt%3DAbstract Authors: Tibullo D, Giallongo C, La Cava P, Berretta S, Stagno F, Chiarenza A, Conticello C, Palumbo GA, Di Raimondo F Imatinib mesylate (IM), a tyrosine kinase inhibitor currently used in chronic myeloid leukemia (CML), may also affect the growth of other cellular systems besides CML cells. Because it has been reported that IM may affect bone tissue remodeling, we evaluated the effects of IM on osteoblastic differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs). After 21 days of culture, hBM-MSCs treated with IM (1 microM) alone or osteogenic medium (OM) + IM showed changes in morphology with evidence of extracellular mineralization and increased mRNA expression of osteogenic markers, such as RUNX2, osteocalcin (OCN), and bone morphogenetic protein (BMP-2). We also obs... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288604 Wed, 25 Mar 2009 23:04:06 +0100 2288604 http://www.medworm.com/index.php?rid=2288603&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302921%26dopt%3DAbstract CONCLUSION: Results obtained in the present study suggest that IgM-producing WM cells not only retained viability in the bone marrow of the WM bone biopsy, but also metastasized to the normal bone marrow of the distant bone implant. The mouse model reported here improves on existing models of WM by recapitulating the adult human bone marrow microenvironment of abnormal WM neoplastic cells. PMID: 19302921 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288603 Wed, 25 Mar 2009 23:03:53 +0100 2288603 http://www.medworm.com/index.php?rid=2288602&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302922%26dopt%3DAbstract CONCLUSIONS: This case demonstrates two different mechanisms by which c-Mpl mutations can impair thrombopoietin signaling, and suggests that mutations in the extracellular domain will not be rescued by c-Mpl agonists if they interfere with normal receptor expression. PMID: 19302922 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288602 Wed, 25 Mar 2009 23:03:45 +0100 2288602 http://www.medworm.com/index.php?rid=2288601&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19302923%26dopt%3DAbstract CONCLUSION: An elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models. PMID: 19302923 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2288601 Wed, 25 Mar 2009 23:03:37 +0100 2288601 http://www.medworm.com/index.php?rid=2190907&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19218011%26dopt%3DAbstract CONCLUSIONS: Our data showed that antibody therapy composed of nonfucosylated rituximab can activate human neutrophil functions involving phagocytosis and MHC class II expression, which may favorably potentiate the adaptive immune response in cancer patients. PMID: 19218011 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2190907 Tue, 17 Feb 2009 15:48:07 +0100 2190907 http://www.medworm.com/index.php?rid=2190906&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19218012%26dopt%3DAbstract CONCLUSION: Runx1 expression play important roles on the proliferation of emerging immature hematopoietic progenitors or the transition process from endothelial to hematopoietic cells presumably by suppressing the genes related to endothelial phenotype. PMID: 19218012 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2190906 Tue, 17 Feb 2009 15:48:02 +0100 2190906 http://www.medworm.com/index.php?rid=2190908&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217201%26dopt%3DAbstract CONCLUSION: Based on our data, VPA is effective at reducing lymphoproliferation in mice, and is currently being studied in a clinical trial as a lympholytic agent in patients with ALPS. PMID: 19217201 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2190908 Wed, 11 Feb 2009 05:00:00 +0100 2190908 http://www.medworm.com/index.php?rid=2188156&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19216019%26dopt%3DAbstract CONCLUSION: Adhesion and direct cell-to-cell contact with an MSC feeder layer supports ex vivo expansion, migratory potential, and stemness of CD133(+) hematopoietic progenitor cells. PMID: 19216019 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2188156 Tue, 10 Feb 2009 05:00:00 +0100 2188156 http://www.medworm.com/index.php?rid=2188155&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19216020%26dopt%3DAbstract CONCLUSION: Perforin deficiency alters T-cell expansion but upregulates T-cell Fas ligand expression. Perforin-mediated cell death appears to play a minor role in mouse models of immune-mediated BM failure. PMID: 19216020 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2188155 Tue, 10 Feb 2009 05:00:00 +0100 2188155 http://www.medworm.com/index.php?rid=2188154&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19216021%26dopt%3DAbstract CONCLUSION: Our findings will be useful in bone marrow transplantations and might explain a body of clinical observations related to LTL distribution in the general population. PMID: 19216021 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2188154 Tue, 10 Feb 2009 05:00:00 +0100 2188154 http://www.medworm.com/index.php?rid=2182901&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19211183%26dopt%3DAbstract CONCLUSIONS: We did not find associations between bone marrow histology and CT45 expression. However, we demonstrated for the first time that positive expression of CT45 was associated with poor prognostic (International Staging System) and poor outcomes in MM patients, meaning that CT45-positive cases presented seven times more chance of worse evolution than the negative ones. PMID: 19211183 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2182901 Mon, 09 Feb 2009 05:00:00 +0100 2182901 http://www.medworm.com/index.php?rid=2101272&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135900%26dopt%3DAbstract CONCLUSION: High-density genomic mapping of CD34+ bone marrow cells from patients with MDS identifies cryptic genetic lesions and offers new opportunities for the discovery of target genes in MDS by integration with gene expression analysis. PMID: 19135900 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101272 Wed, 14 Jan 2009 08:49:24 +0100 2101272 http://www.medworm.com/index.php?rid=2101271&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135901%26dopt%3DAbstract CONCLUSIONS: The NBEA gene at 13q13, and its expression are frequently disrupted in MM. Additional studies are warranted to evaluate the role of NBEA as a novel candidate tumor-suppressor gene. PMID: 19135901 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101271 Wed, 14 Jan 2009 08:49:16 +0100 2101271 http://www.medworm.com/index.php?rid=2101270&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135902%26dopt%3DAbstract CONCLUSION: TPA induces the expression of several miRNAs in HL-60 cells, one such miRNA (miR-320) contributes to downregulation of TfR-1 surface expression characteristically seen during HL-60 monocytic differentiation. Moreover, TfR-1-targeting miRNAs, such as miR-320, may have potential as novel therapeutic agents for cancer due to their inhibitory effects on cell proliferation. PMID: 19135902 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101270 Wed, 14 Jan 2009 08:49:11 +0100 2101270 http://www.medworm.com/index.php?rid=2101269&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19136196%26dopt%3DAbstract CONCLUSION: The presumed autoaggressive T cells were not confirmed in MDS in our experimental arrangement. Alloreactivity studies demonstrated the impairment of effector cytotoxic phase of cell-mediated immunological reaction in MDS, namely in RA. The significance of our finding of defective cytotoxicity for pathogenesis, clinical course, and even for therapy is discussed together with other immunological defects reported so far. PMID: 19136196 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101269 Fri, 09 Jan 2009 05:00:00 +0100 2101269 http://www.medworm.com/index.php?rid=2101276&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135770%26dopt%3DAbstract CONCLUSION: The serum-free culturing system may be a valuable instrument in the study of ALL cell biology, as well as in the evaluation of novel targeted therapeutics. PMID: 19135770 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101276 Thu, 08 Jan 2009 05:00:00 +0100 2101276 http://www.medworm.com/index.php?rid=2101275&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135771%26dopt%3DAbstract CONCLUSIONS: Our data suggest that the early cellular and transcriptional effects of P190 BCR/ABL1 and P210 BCR/ABL1 expression are very similar when they are expressed in the same human progenitor cell population, and that STAT5 is an important regulator of BCR/ABL1-induced erythroid cell expansion. PMID: 19135771 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101275 Thu, 08 Jan 2009 05:00:00 +0100 2101275 http://www.medworm.com/index.php?rid=2101274&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135772%26dopt%3DAbstract CONCLUSION: We have developed an accurate ELISA method to measure BCR-ABL activity within Ph(+) cells, which is comparable to other in vitro BCR-ABL assessment techniques in terms of sensitivity and could be adapted for high throughput. PMID: 19135772 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101274 Thu, 08 Jan 2009 05:00:00 +0100 2101274 http://www.medworm.com/index.php?rid=2101273&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135773%26dopt%3DAbstract Authors: Mercier F, Monczak Y, François M, Prchal J, Galipeau J Myeloproliferative disorders (MPDs) are often associated with the presence of the JAK2-V617F mutation in hematopoietic cells. It is currently not known if this mutation is carried as well by bone marrow mesenchymal stromal cells (MSCs) in these patients. To test this hypothesis, we recruited seven patients with JAK2-V617F(+) MPD, isolated marrow MSCs and characterized their phenotype and mesenchymal differentiation capacity, and probed for JAK2-V617F genomic DNA mutation. We found that MSCs of most patients could be culture-expanded and had a phenotype and differentiation capacity similar to that of MSCs derived from normal subjects. Using real-time polymerase chain reaction and melting curve analysis with probes spec... Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2101273 Thu, 08 Jan 2009 05:00:00 +0100 2101273 http://www.medworm.com/index.php?rid=2061285&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19100521%26dopt%3DAbstract CONCLUSION: These findings suggest that targeted disruption of key signaling pathways in combination with the tyrosine kinase activity of leukemic oncogenes, such as Tel/PDGFRbeta, may result in more efficacious therapies for suppressing leukemic progression in the clinical setting. PMID: 19100521 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061285 Wed, 24 Dec 2008 12:47:41 +0100 2061285 http://www.medworm.com/index.php?rid=2061284&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19100522%26dopt%3DAbstract CONCLUSION: In B cells, Erk activation leads directly to phosphorylation of Bim on sites associated with activation of Bim. In Toledo B-NHL cells, the dependence of apoptosis on sustained signaling suggests that Bcl-2 family members could interpret signal duration, an important determinant of B cell receptor-mediated negative selection. Certain cases of B-NHL might respond to DAG analogue treatment by the mechanism outlined here. PMID: 19100522 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061284 Wed, 24 Dec 2008 12:47:37 +0100 2061284 http://www.medworm.com/index.php?rid=2061283&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19100523%26dopt%3DAbstract CONCLUSIONS: Quantitative assessment of NPM1Amut seems to provide a reliable MRD marker in the posttransplantation period, predicting relapse earlier than morphology or molecular chimerism, which should be confirmed in larger studies. PMID: 19100523 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061283 Wed, 24 Dec 2008 12:47:34 +0100 2061283 http://www.medworm.com/index.php?rid=2061282&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19100524%26dopt%3DAbstract CONCLUSION: Neither postgrafting vaccination nor graft augmentation with MoDC were effective in supporting durable engraftment. Additional modifications are necessary to improve potential strategies aimed at establishment of early tissue specific graft-vs-host reactions. PMID: 19100524 [PubMed - in process] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061282 Wed, 24 Dec 2008 12:47:29 +0100 2061282 http://www.medworm.com/index.php?rid=2061276&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19101069%26dopt%3DAbstract CONCLUSION: Wnt16 does not activate canonical Wnt signaling in E2A-PBX1-positive cells. Instead, beta-catenin is involved in N-cadherin-dependent adherence junctions, suggesting for the first time that leukemia-stroma interactions may be mediated via an N-cadherin-dependent mechanism. PMID: 19101069 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061276 Thu, 18 Dec 2008 05:00:00 +0100 2061276 http://www.medworm.com/index.php?rid=2061275&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19101070%26dopt%3DAbstract CONCLUSION: We find no evidence for an association of ABCG2 with SP activity or hematopoietic progenitor function in hUCB. PMID: 19101070 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061275 Thu, 18 Dec 2008 05:00:00 +0100 2061275 http://www.medworm.com/index.php?rid=2061281&cid=s_35567_19_f&fid=35567&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19100675%26dopt%3DAbstract CONCLUSION: EPO protects erythroid cells from apoptosis in part through ERK-mediated phosphorylation followed by proteasomal degradation of BIM. PMID: 19100675 [PubMed - as supplied by publisher] (Source: Experimental Hematology) Experimental Hematology journals http://www.medworm.com/rss/comments.php?id=2061281 Wed, 17 Dec 2008 05:00:00 +0100 2061281