MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Experimental and Molecular Pathology' source. Thu, 09 Feb 2012 00:42:11 +0100 http://www.medworm.com/index.php?rid=5630705&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22273483%26dopt%3DAbstract Authors: Oliva J, French SW Abstract Mallory-Denk body (MDB) formation is a component of alcoholic and non alcoholic hepatitis. Proteins of the TLR pathway were shown to be involved in the formation of MDBs, in mice fed DDC. TLR genes are upregulated and SAMe supplementation prevents this up regulation and prevented the formation of MDBs. DNA of livers from control mice, from mice fed DDC 10weeks, refed 1week with DDC and with DDC+SAMe were extracted and used to study the methylation pattern of genes involves in the TLR pathway. A PCR array was used to analyze it. Using PCR arrays for the mouse TLR pathway,24 genes were found whose expression of IL12A was regulated by the methylation of its gene. DDC fed for 10weeks reduced the methylation of the IL12A gene expression. This express... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5630705 Mon, 16 Jan 2012 05:00:00 +0100 5630705 http://www.medworm.com/index.php?rid=5587044&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227376%26dopt%3DAbstract CONCLUSION: Post-irradiation damage to the ileum was delayed and followed by an altered repair process with structural changes of the villi. The observed changes might result from a higher sensitivity to oxidative stress mechanisms with subsequent damage of the regenerative capacity of the crypt-villus axis, accompanied by a sustained "inflammatory response" and vascular damage with a lack of regeneratory cell recruitment. PMID: 22227376 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5587044 Thu, 29 Dec 2011 05:00:00 +0100 5587044 http://www.medworm.com/index.php?rid=5502324&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22138105%26dopt%3DAbstract Authors: Shanklin DR Abstract Normally developed thyroid function is critical to the transition from fetal to neonatal life with the onset of independent thermoregulation, the most conspicuous of the many ways in which thyroid secretions act throughout the body. A role for thyroid secretions in growth and maturation of the lungs as part of the preparation for the onset of breathing has been recognized for some time but how this contributes to tissue and cell processes and defenses under the duress of respiratory distress has not been well examined. Extensive archival autopsy material was searched for thyroid and adrenal weights, first by gestational age, and then for changes during the first hours after birth as ratios to body weight. After a gestational age of 22weeks the fetal th... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5502324 Fri, 25 Nov 2011 05:00:00 +0100 5502324 http://www.medworm.com/index.php?rid=5502323&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22146696%26dopt%3DAbstract Authors: Saugspier M, Dorn C, Thasler WE, Gehrig M, Heilmann J, Hellerbrand C Abstract Female inflorescences of the hop plant Humulus lupulus L. contain a variety of secondary metabolites with bitter acids (BA) as quantitatively dominating secondary metabolites. The use of hops in beer brewing has a long history due to the antibacterial effects of the BA and their typical bitter taste. Furthermore, hop cones are used in traditional medicine and for pharmaceutical purposes. Recent studies indicate that BA may affect activity of the transcription factor NFκB. NFκB plays a key role in the activation process of hepatic stellate cells (HSC), which is the key event of hepatic fibrosis. The aim of this study was to investigate the effect of BA on HSC (activation) and their potential to ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5502323 Fri, 25 Nov 2011 05:00:00 +0100 5502323 http://www.medworm.com/index.php?rid=5502325&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22120785%26dopt%3DAbstract Propagation of ovine prions from "poor" transmitter scrapie isolates in ovine PrP transgenic mice. Exp Mol Pathol. 2011 Nov 19; Authors: Thackray AM, Hopkins L, Lockey R, Spiropoulos J, Bujdoso R Abstract Ovine prion strains have typically been identified by their transmission properties, which include incubation time and lesion profile, in wild type mice. The existence of scrapie isolates that do not propagate in wild type mice, defined here as "poor" transmitters, are problematic for conventional prion strain typing studies as no incubation time or neuropathology can be recorded. This may arise because of the presence of an ovine prion strain within the original inoculum that does not normally cross the species barrier into wild type mice or the presence of a low dose of ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5502325 Sat, 19 Nov 2011 05:00:00 +0100 5502325 http://www.medworm.com/index.php?rid=5449236&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22115939%26dopt%3DAbstract Authors: Suzuki S, Kurata M, Abe S, Miyazawa R, Murayama T, Hidaka M, Yamamoto K, Kitagawa M Abstract Myelodysplastic syndromes (MDS) are characterized by proliferation and apoptosis of bone marrow cells. Minichromosome maintenance protein (MCM) 2, which is known to be essential for regulating DNA replication, has proven to have a pro-apoptotic effect in our recent study. Thus, to determine the role of MCM2 in MDS, real-time PCR, immunohistochemistry and in vitro analysis were performed. Our results showed higher MCM2 expression in MDS than in control and AML. Notably, there was no correlation between MCM2 and Ki67-labeling indices (LIs) in MDS, while MCM2 LIs were significantly correlated with cleaved caspase 3 LIs in MDS. In vitro analysis revealed that MCM2 overexpression induce... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449236 Tue, 15 Nov 2011 05:00:00 +0100 5449236 http://www.medworm.com/index.php?rid=5449238&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22101258%26dopt%3DAbstract CONCLUSIONS: LPCAT1 correlates with the progression of prostate cancer and could be a new biomarker in diagnosis, prognosis and studying the pathogenesis of prostate cancer. PMID: 22101258 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449238 Fri, 11 Nov 2011 05:00:00 +0100 5449238 http://www.medworm.com/index.php?rid=5449241&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22100877%26dopt%3DAbstract Authors: Singleterry WL, Henderson H, Cruse JM Abstract In this present investigation, flow cytometry was utilized to evaluate 13 healthy controls and 31 HIV-1 infected patients who had advanced to the AIDS stage of infection (CD4 count below 200cells/mm(3)), for the expression of CD161 on CD3(+) double negative (DN) (CD3(+)CD4(-)CD8(-)) T cells, CD4(+) T cells, CD8(+) T cells and γδ T cells. The observed depletion of CD161(+) T cells from peripheral circulation was due primarily to the loss of CD4(+)CD161(+) T cells; as these cells represented 8.67±0.74% of the total healthy control peripheral T cell population, while the CD4(+)CD161(+) T cells of the AIDS group represented only 3.35±0.41% (p=<0.0001) of the total peripheral T cell population. We have also shown here that t... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449241 Thu, 10 Nov 2011 05:00:00 +0100 5449241 http://www.medworm.com/index.php?rid=5449240&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22101256%26dopt%3DAbstract CONCLUSION: ALDH1 expression pattern is an independent prognostic marker for survival of T3 N0 M0 G2 colorectal cancer patients. PMID: 22101256 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449240 Thu, 10 Nov 2011 05:00:00 +0100 5449240 http://www.medworm.com/index.php?rid=5449239&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22101257%26dopt%3DAbstract Authors: Melnick M, Sedghizadeh PP, Allen CM, Jaskoll T Abstract Human cytomegalovirus (hCMV) infection is common. Although still controversial, there is growing evidence that active hCMV infection is associated with a variety of malignancies, including brain, breast, lung, colon, and prostate. Given that hCMV is frequently resident in salivary gland (SG) ductal epithelium, we hypothesized that hCMV would be important to the pathogenesis of SG mucoepidermoid carcinoma (MEC). This was initially supported by our finding that purified CMV induces malignant transformation in SG cells in an in vitro mouse model, and utilizes a pathogenic pathway previously reported for human MEC. Here we present the histologic and molecular characterizations of 39 human SG MECs selected randomly from a ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449239 Thu, 10 Nov 2011 05:00:00 +0100 5449239 http://www.medworm.com/index.php?rid=5449237&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22101259%26dopt%3DAbstract Authors: Ayala P, Montenegro J, Vivar R, Letelier A, Urroz PA, Copaja M, Pivet D, Humeres C, Troncoso R, Vicencio JM, Lavandero S, Araya GD Abstract Increasing evidence indicates that endoplasmic reticulum (ER) stress is involved in various diseases. In the human heart, ischemia/reperfusion has been correlated to ER stress, and several markers of the unfolded protein response (UPR) participate during cardiac remodeling and fibrosis. Here, we used isoproterenol (ISO) injection as a model for in vivo cardiac fibrosis. ISO induced significant cardiomyocyte loss and collagen deposition in the damaged areas of the endocardium. These responses were accompanied by an increase in the protein levels of the luminal ER chaperones BIP and PDI, as well as an increase in the UPR effector CHOP. T... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5449237 Thu, 10 Nov 2011 05:00:00 +0100 5449237 http://www.medworm.com/index.php?rid=5430658&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22079478%26dopt%3DAbstract CONCLUSION: This was the first report on experimental SSM-CVB3 infections in macaques with serious hepatic and renal damage, except for myocarditis. The information obtained from this study suggests that the SSM-CVB3 strain and this macaque model could be used for studying CVB3-induced cardiac, hepatic or renal diseases. PMID: 22079478 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5430658 Mon, 07 Nov 2011 05:00:00 +0100 5430658 http://www.medworm.com/index.php?rid=5430657&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22079479%26dopt%3DAbstract Authors: Hoffman WH, Shacka JJ, Andjelkovic AV Abstract Semi-quantitative neuroradiologic studies, quantitative neuron density studies and immunocytochemistry markers of oxidative stress and neuroinflammation indicate neuronal injury and deficits in young patients with chronic poorly controlled type 1 diabetes mellitus (T1DM). Present data suggest that pathogenesis of the neuronal deficits in young patients, who die as the result of diabetic ketoacidosis (DKA) and brain edema (BE), does not involve apoptosis, a prominent form of regulated cell death in many disease states. To further address this we studied mediators of macroautophagy, endoplasmic reticulum (ER) stress and apoptosis. In all areas studied we demonstrated increased levels of macroautophagy-associated proteins includi... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5430657 Sun, 06 Nov 2011 04:00:00 +0100 5430657 http://www.medworm.com/index.php?rid=5430659&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22075155%26dopt%3DAbstract We examined the role of CD43 glycoprotein in a colorectal carcinoma cell line. We expressed human CD43 antigen on HT-29 cells, a colon adenocarcinoma cell line, and compared the adhesion to the extracellular matrix with that of mock-transduced cells in vitro. CD43 expression inhibited the adhesion to extracellular matrix, such as collagen type IV and laminin. As the expression of β1 integrin was downregulated in CD43-expressing HT-29 cells, the anti-adhesive effect of CD43 might be implicated in its expression. Our findings suggest that the anti-adhesive function of CD43 in colon carcinoma cells plays a role in the tumorigenesis and metastasis of colorectal carcinoma cells. PMID: 22075155 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5430659 Sat, 29 Oct 2011 04:00:00 +0100 5430659 http://www.medworm.com/index.php?rid=5364780&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22044461%26dopt%3DAbstract Authors: Sharma M, Blackman MR, Sharma MC Abstract Activation of the fibrinolytic pathway has long been associated with human breast cancer. Plasmin is the major end product of the fibrinolytic pathway and is critical for normal physiological functions. The mechanism by which plasmin is generated in breast cancer is not yet fully described. We previously identified annexin II (ANX II), a fibrinolytic receptor, in human breast tumor tissue samples and observed a strong positive correlation with advanced stage cancer (Sharma et al., 2006a). We further demonstrated that tissue plasminogen activator (tPA) binds to ANX II in invasive breast cancer MDA-MB231cells, which leads to plasmin generation (Sharma et al., 2010b). We hypothesize that ANX II-dependent plasmin generation in breast t... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364780 Tue, 25 Oct 2011 04:00:00 +0100 5364780 http://www.medworm.com/index.php?rid=5364782&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22037282%26dopt%3DAbstract CONCLUSIONS: These data show that human SFs express both chemerin and its receptor. As chemerin enhanced expression of TLR4 and induced release of CCL2 in SFs, a role of this protein in innate immune system-associated joint inflammation is proposed. PMID: 22037282 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364782 Wed, 19 Oct 2011 04:00:00 +0100 5364782 http://www.medworm.com/index.php?rid=5364781&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22037714%26dopt%3DAbstract This study aimed to investigate the expression of drug resistance (MDR1, MRP1, LRP, BCRP, GSTP1, DHFR) and apoptotic genes (p53, BCL-2, Survivin) in adult acute leukemias and compare them with clinical and hematological findings and response to induction chemotherapy. Eighty-five patients with AL [45 with acute myeloid leukemia (AML) and 40 with acute lymphoblastic leukemia (ALL)] were used as a study group. Real-time PCR results showed that expression level of MDR1 was significantly higher in AML whereas expression of DHFR, BCRP and Survivin was significantly higher in ALL patients. In AML, significant correlation was observed between LRP and MRP1 (r(s)=0.44, p=0.016), LRP and DHFR (r(s)=0.41, p=0.02), MDR1 and BCL-2 (r(s)=0.38, p=0.03). Expression of GSTP1 and LRP correlated with high wh... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364781 Wed, 19 Oct 2011 04:00:00 +0100 5364781 http://www.medworm.com/index.php?rid=5364783&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22032939%26dopt%3DAbstract Authors: Ridinger M, Köhl P, Gäbele E, Wodarz N, Schmitz G, Kiefer P, Hellerbrand C Abstract An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. Furthermore, CDT is employed as a marker of abstinence. Here, we analyzed CDT in patients with chronic excessive alcohol abuse at the beginning and during abstinence. Twenty-nine alcohol dependent patients were recruited from an in-patient abstention program. Reported drinking levels were at least 100g/d (range up to 450g/d; mean: 248.9±94.7g/d) within the last month before study entry. Blood samples were drawn at the beginning and during the abstention program and the relative concentration (%CDT) of C... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364783 Tue, 18 Oct 2011 04:00:00 +0100 5364783 http://www.medworm.com/index.php?rid=5364786&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22024355%26dopt%3DAbstract Authors: Maegdefrau U, Bosserhoff AK Abstract Several of the different bone morphogenetic proteins (BMPs) are involved in development and progression of specific tumors. For hepatocellular carcinoma (HCC) only BMP4 and BMP6 are described to be important for carcinogenesis. However, up to now neither the influence of other BMPs on tumor progression, nor the responsible signaling pathways to mediate target gene expression in HCC are known. In order to characterize BMP expression pattern in HCC cell lines, we performed RT-PCR analysis and revealed enhanced expression levels of several BMPs (BMP4, 6, 7, 8, 9, 10, 11, 13 and 15) in HCC. Thus, we treated HCC cells with the general BMP inhibitors chordin and noggin to determine the functional relevance of BMP overexpression and observed d... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364786 Sat, 15 Oct 2011 04:00:00 +0100 5364786 http://www.medworm.com/index.php?rid=5364785&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22032937%26dopt%3DAbstract This study showed the inhibition of the proliferation and increased necrosis in response to SAMe on liver cancer cell lines Hepa 1-6 and C34. PMID: 22032937 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364785 Sat, 15 Oct 2011 04:00:00 +0100 5364785 http://www.medworm.com/index.php?rid=5364784&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22032938%26dopt%3DAbstract In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells (PBMCs) by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. Furthermore, we screened HLA-A24-restricted HPV16 E6- and E7-derived peptides, and found that one E6-derived peptide (E6 66-74) showed the highest immunogenicity with ELISPOT assay from 100% of HPV16-positive patients (4 out of 4). On the other hand, other E6- or E7-derived peptides, including E6 49-57, E6 82-90, E6 87... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364784 Sat, 15 Oct 2011 04:00:00 +0100 5364784 http://www.medworm.com/index.php?rid=5364787&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22019339%26dopt%3DAbstract CONCLUSIONS: We hypothesize that MGMT and PTEN expressions have prognostic significance in breast cancer. Also, based on their predictive value of response to therapy, evaluating MGMT and PTEN and learning to interpret their patterns of immunoexpression will undoubtedly lead to a greater understanding of breast cancer and its treatment. PMID: 22019339 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364787 Wed, 12 Oct 2011 04:00:00 +0100 5364787 http://www.medworm.com/index.php?rid=5364788&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22015804%26dopt%3DAbstract In this study we predicted the 3D (three dimensional) structure of Dectin-1 receptor based on homology modeling using MODELLER 9v8 software. The TMHMM server was used for the prediction of transmembrane helices. DALI, PROFUNC, Q-Site Finder, PINTS servers and PASS software used for the prediction of functional sites in the modeled Dectin-1 receptor. The docking investigation of Dectin-1 receptor with β-glucan suggests that ASP150, ASP113, GLY106, and GLU196 amino acids are the catalytic residues which form a shallow groove in the protein surface and bind to ligand β-glucan. We hope that this work will help in in-silico screening, structure-based design, and in understanding the structural basis of ligand binding to the Dectin-1 receptor. PMID: 22015804 [PubMed - as supplied by publis... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5364788 Sat, 08 Oct 2011 04:00:00 +0100 5364788 http://www.medworm.com/index.php?rid=5336025&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22001380%26dopt%3DAbstract Authors: Morohoshi K, Patel N, Ohbayashi M, Chong V, Grossniklaus HE, Bird AC, Ono SJ Abstract Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in industrial counties. Its pathogenesis is at least partially mediated by immunological factors, including a possible autoimmune response. To date, only a few antibodies have been identified in sera from patients with AMD. In order to reveal an autoantibody profile for AMD and identify biomarkers for progression of this disease, we have performed an antigen microarray analysis of serum samples from patients with AMD and healthy controls. Sera from the AMD groups contained high levels of IgG and IgM autoantibodies to some systemic antigens when compared to the normal group. Targeted antigens included cyc... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5336025 Thu, 06 Oct 2011 04:00:00 +0100 5336025 http://www.medworm.com/index.php?rid=5336024&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22001602%26dopt%3DAbstract In this study, we compared the efficiencies of G2/M-related antigens including Birc5, Aurka, Nke2 and Plk1 by using a DNA vaccination model. Mice that had been immunized with G2/M-related antigens coding plasmid were challenged with CT26 colon cancer cells. Interestingly, Birc5- and Aurka-immunized mice showed an anti-tumor effect, whereas Nek2- and Plk1-immunized mice did not show any anti-tumor effect. We investigated the expression of G2/M-related antigens in cancer stem-like cell (CSC)/cancer-initiating cell (CIC) population to verify the difference in the anti-tumor effect. CSCs/CICs were isolated as side population (SP) cells using Hoechst 33342 dye from CT 26 cells. It was found that Birc5 and Aurka are expressed in both CSCs/CICs and non-CSCs/CICs (shared antigens), whereas Nek2 an... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5336024 Sun, 02 Oct 2011 04:00:00 +0100 5336024 http://www.medworm.com/index.php?rid=5288131&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21497601%26dopt%3DAbstract This study aims to characterize the immune cell population in patients from our community suffering from systemic lupus erythematosus and to prove that these patients exhibit a unique cellular profile compared to healthy age, race and gender matched control subjects. Surprisingly, our findings demonstrate that patients from the local Mississippi area exhibit increased proportions of CD25(+) FoxP3(+) regulatory T cells and CD25(+) FoxP3(-) T cells (of CD45(+) CD3(+) CD4(+) helper T cells) as compared to healthy controls. HLA tissue-typing of these lupus patients revealed a prominent subgroup (~30%) of patients possessing the HLA DRB1*1503 allele. The investigation of this subgroup demonstrated regulatory T cell composition similar to that of the total lupus group and to that of the non-HLA ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288131 Sat, 01 Oct 2011 04:00:00 +0100 5288131 http://www.medworm.com/index.php?rid=5288130&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21801719%26dopt%3DAbstract CONCLUSIONS: Increased cyclins A, E, D1, CDK2 and CDK4 expression is important for HCV-associated CH and HCC. Cyclin D1 and cyclin A are prognostic biomarkers associated with reduced OS in HCC. Cyclin D1 aberration could identify high risk groups of CH patients prone to develop HCC. PMID: 21801719 [PubMed - in process] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288130 Sat, 01 Oct 2011 04:00:00 +0100 5288130 http://www.medworm.com/index.php?rid=5288129&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21801720%26dopt%3DAbstract CONCLUSIONS: Our findings suggest that the ApoB/ApoA 1 ratio is strongly associated with MetS and its components, as well as with IR. PMID: 21801720 [PubMed - in process] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288129 Sat, 01 Oct 2011 04:00:00 +0100 5288129 http://www.medworm.com/index.php?rid=5288128&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21806986%26dopt%3DAbstract In this study, a pair of shared primers was designed for α2 and α1 gene but with length-different amplicons (159 bp and 409 bp). On the dissociation curve analysis profile after PCR, there shows two obvious peaks which represent the two different amplicons. Relative copy number of α2 and α1 gene can be deduced from the ratio of the two peaks. A comprehensive diagnosis for α-thalassemia 10 genotypes of deletions can be achieved when combined with a single-tube duplex PCR for detecting --SEA and non-deletional alleles of αα or α(T)α. Besides, a single-tube multiplex PCR, which is a cost-effective version of dual-priming-oligonucleotide based system, was designed for two common mutations of α-thalassemia in China (Hb Constant Spring and Hb Quong Sze), and these two mutations can be ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288128 Sat, 01 Oct 2011 04:00:00 +0100 5288128 http://www.medworm.com/index.php?rid=5288127&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21963599%26dopt%3DAbstract Authors: Tilli TM, Thuler LC, Matos AR, Coutinho-Camillo CM, Soares FA, da Silva EA, Neves AF, Goulart LR, Gimba ER Abstract Osteopontin splicing isoforms (OPN-SI) present differential expression patterns and specific tumor roles. Our aims were to characterize OPN-SI expression in prostate cancer (PCa) and benign prostate hyperplasia (BPH) tissues, besides evaluating their potential as biomarkers for PCa diagnosis and prognostic implications. Prostatic tissue specimens were obtained from 40 PCa and 30 benign prostate hyperplasia (BPH) patients. Quantitative real time PCR (qRT-PCR) was used to measure OPN-SI mRNA expression. Immunohistochemical analysis was performed using an anti-OPNc polyclonal antibody. Biostatistical analyses evaluated the association of OPN-SI and total Prostat... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288127 Thu, 22 Sep 2011 04:00:00 +0100 5288127 http://www.medworm.com/index.php?rid=5288125&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21963611%26dopt%3DAbstract Authors: Shao Z, Schäffler A, Hamer O, Dickopf J, Goetz A, Landfried K, Voelk M, Kopp A, Herfarth H, Karrasch T, Brünnler T, Koh LK, Buechler C, Schwarz H Abstract The progression of acute pancreatitis to necrotizing pancreatitis which often results in high morbidity and mortality is difficult to predict. Here we report that serum concentrations of sCD137 are increased in patients with acute pancreatitis. Admission levels and 10-day median sCD137 levels positively correlate with markers of biliary pancreatitis and the 10-day sCD137 median is significantly higher in metabolic than in alcoholic pancreatitis. Serum concentrations of sCD137 at time of admission and the 10-day median of sCD137 correlate with the Ranson and APACHE II disease scores but not with the radiological Balthaz... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288125 Thu, 22 Sep 2011 04:00:00 +0100 5288125 http://www.medworm.com/index.php?rid=5288126&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21963600%26dopt%3DAbstract In conclusion, the Molecular Fixative regardless of fixation length, and the rapid tissue processing system were able to preserve large DNA and RNA fragments in paraffin blocks, making these techniques preferable for use in downstream molecular diagnostic assays. PMID: 21963600 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5288126 Wed, 21 Sep 2011 04:00:00 +0100 5288126 http://www.medworm.com/index.php?rid=5270229&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21946149%26dopt%3DAbstract Authors: Walter R, Wanninger J, Bauer S, Eisinger K, Neumeier M, Weiss TS, Amann T, Hellerbrand C, Schäffler A, Schölmerich J, Buechler C Abstract Connective tissue growth factor (CTGF) is induced in liver fibrosis and enhances the activity of transforming growth factor β (TGFβ). Recently we have shown that the hepatoprotective adipokine adiponectin downregulates CTGF in primary human hepatocytes (PHH). In the current study, the mechanisms mediating suppression of CTGF by adiponectin and the well described downstream effector of adiponectin receptor 2 (AdipoR2), peroxisome proliferator activated receptor α (PPARα), were analyzed in more detail. Adiponectin downregulated CTGF mRNA and protein in primary human hepatocytes (PHH) and suppression was blocked by a PPARα antagonist... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5270229 Sat, 17 Sep 2011 04:00:00 +0100 5270229 http://www.medworm.com/index.php?rid=5270232&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945733%26dopt%3DAbstract Authors: Gruber HE, Hoelscher GL, Ingram JA, Hanley EN Abstract Matrix metalloproteinase (MMP) regulation and expression is important in the aging/degenerating human intervertebral disc. MMP-26 (also known as matrilysin-2 or endometase) is a newly discovered MMP which degrades type IV collagen, fibronectin, fibrinogen, vitronectin, denatured collagen types I-IV, insulin-like growth factor binding protein 1, and activated pro-MMP-9. Our objective here was to determine if it is present in human disc tissue and cultured disc cells. Immunohistochemistry and microarray gene expression analyses were used to evaluate the presence of MMP-26 in human disc tissue from healthy and degenerated discs. Immunohistochemistry was also applied to human annulus cells cultured in a collagen sponge. Ce... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5270232 Wed, 14 Sep 2011 04:00:00 +0100 5270232 http://www.medworm.com/index.php?rid=5270231&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945735%26dopt%3DAbstract Authors: Darr CM, Castorena J, Korampally V, Gangopadhyay S, Polo-Parada L Abstract The application of commercially available microarray slides as substrates for fluorogenic protease assays has been explored in terms of binding efficiency, stability, and activity. A fluorescent, biotinylated substrate for botulinum neurotoxin A (BoNTA) was attached via self-assembled monolayer of Streptavidin to amine-reactive aldehyde, epoxy, hydrogel, and polymer slides. Nexterion Slide P® was found to have optimal protein binding efficiency and stability of the slides examined. Addition of glycerol to the printing buffer improved spot morphology significantly and polyvinylpyrrolidone provided long-term stability, allowing chips to be stored for up to 1month with good viability. Detection of a r... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5270231 Wed, 14 Sep 2011 04:00:00 +0100 5270231 http://www.medworm.com/index.php?rid=5270230&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21945736%26dopt%3DAbstract In this study, we investigated the effect of the CD28/B7 antagonist, CTLA-4Ig, on the lung inflammation and the T cell subset profile in experimental Saccharopolyspora recivirgula (SR)-induced HP. C57BL/6 mice were treated with SR or saline during two and three weeks and in addition of CTLA-4Ig was administrated after either the second or third week and mice were sacrificed seven days later. The extent of the lung inflammation was quantified by histopathology and the lung T cell subsets (Treg, Th17, γδT and NKT) were analyzed by flow cytometry. Mice treated with CTLA-4Ig showed a significant decrease in the extent of lung damage (p<0.05), and exhibited a decreased number of inflammatory cells in the bronchoalveolar lavage (BAL) with diminished CD4/CD8 T cell ratio. Also, a significant... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5270230 Wed, 14 Sep 2011 04:00:00 +0100 5270230 http://www.medworm.com/index.php?rid=5236356&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21930124%26dopt%3DAbstract Authors: Jiang Y, Huang Y, Cheng C, Lu W, Zhang Y, Liu X, Zou L, Ben Q, Shen A Abstract No proven targeted therapy is currently available for the treatment of triple-negative breast cancer (TNBC). Ligand activation of peroxisome-activated receptor (PPAR)γ induces antitumor effects in cancer but not obviously in TNBC. In TNBC cells, combined treatment with thiazolidinedione and demethylation drugs Hydralazine up-regulated protein and mRNA levels of PPARγ. Besides the combination of two drugs promote antiproliferative and apoptotic effects in TNBC cells and decrease the proliferation index in the tumor xenografts. Taken together, our results suggest that multidrug regimens including a combination of Thiazolidinedione and Hydralazine may provide a therapeutic advantage in TNBC. ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5236356 Sat, 10 Sep 2011 04:00:00 +0100 5236356 http://www.medworm.com/index.php?rid=5236357&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21930123%26dopt%3DAbstract CONCLUSION: The ADAM33 gene plays an important role in facilitating susceptibility to childhood asthma in this Han Chinese population. PMID: 21930123 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5236357 Fri, 09 Sep 2011 04:00:00 +0100 5236357 http://www.medworm.com/index.php?rid=5236355&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21930125%26dopt%3DAbstract Authors: Castilho-Fernandes A, de Almeida DC, Fontes AM, Melo FU, Picanço-Castro V, Freitas MC, Orellana MD, Palma PV, Hackett PB, Friedman SL, Covas DT Abstract The LX-2 cell line has characteristics of hepatic stellate cells (HSCs), which are considered pericytes of the hepatic microcirculatory system. Recent studies have suggested that HSCs might have mesenchymal origin. We have performed an extensive characterization of the LX-2 cells and have compared their features with those of mesenchymal cells. Our data show that LX-2 cells have a phenotype resembling activated HSCs as well as bone marrow-derived mesenchymal stem cells (BM-MSCs). Our immunophenotypic analysis showed that LX-2 cells are positive for activated HSC markers (αSMA, GFAP, nestin and CD271) and classical mesenc... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5236355 Fri, 09 Sep 2011 04:00:00 +0100 5236355 http://www.medworm.com/index.php?rid=5236354&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21930126%26dopt%3DAbstract Authors: Kanter M Abstract The aim of this study was to investigate the protective effect of Ginkgo biloba (EGb 761) on testicular torsion/detorsion induced ischemia-reperfusion (I/R) injury. A total of 24 male Wistar albino rats were divided into three groups: control, I/R and I/R treated with EGb 761; each group contains 8 animals. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischemia period was 5h and orchiectomy was performed after 5h of detorsion. EGb 761 (50mg/kg, orally) was administrated only once, 40min prior to detorsion. To date, no more histopathological changes on testicular torsion/detorsion induced ischemia-reperfusion (I/R) injury in rats by EGb 761 treatment have been reported. Spermatogenesis and mean seminiferous ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5236354 Fri, 09 Sep 2011 04:00:00 +0100 5236354 http://www.medworm.com/index.php?rid=5207364&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884695%26dopt%3DAbstract This study was aimed to evaluate the clinical value of plasma methylation analysis of a panel of four genes (APC, GSTP1, RASSF1A, and SFRP1), which was identified by our previous work, for the noninvasive diagnosis of hepatocellular carcinoma (HCC). The methylation status of these four genes in 150 plasma samples from 72 patients with HCC, 37 benign live diseases and 41 normal controls was detected with methylation-sensitive restriction enzymes-based quantitative PCR (MSRE-qPCR) method. The plasma methylation levels of APC, GSTP1, RASSF1A, and SFRP1 were significantly higher in HCCs than those in normal or benign controls (P<0.05). Although the area under the receiver-operation characteristic curve (AUC-ROC) for individual gene was moderate (range, from 0.800 to 0.881), the combination ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5207364 Mon, 22 Aug 2011 04:00:00 +0100 5207364 http://www.medworm.com/index.php?rid=5169358&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21855539%26dopt%3DAbstract CONCLUSIONS: Chronic alcohol consumption induced a Th2 response within the Th1/Th2 balance. Th2 response is one of the underlying mechanism involved in alcohol-induced cardiac fibrosis. PMID: 21855539 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5169358 Mon, 08 Aug 2011 23:00:00 +0100 5169358 http://www.medworm.com/index.php?rid=5169359&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21855538%26dopt%3DAbstract In conclusion, our data indicate both the liver and adipose tissue as cellular sources of elevated circulating MCP-1 levels already in the early phase of hepatic steatosis. Since MCP-1 derived from visceral adipose tissue reaches the liver via portal circulation at high concentrations it may significantly contribute to the progression of simple steatosis to NASH. PMID: 21855538 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5169359 Sat, 06 Aug 2011 23:00:00 +0100 5169359 http://www.medworm.com/index.php?rid=5169354&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21855540%26dopt%3DAbstract Authors: Hazard SW, Myers RL, Ehrlich HP Abstract Severed tendons can undergo regenerative healing, intrinsic tendon repair. Fibrillogenesis of chick tendon involves "collagen fibril segments" (CFS), which are the building blocks of collagen fibers that make up tendon fascicles. The CFS are 10.5 micron in length, composed of tropocollagen monomers arranged in parallel arrays. Rather than incorporating single tropocollagen molecules into growing collagen fibers, incorporating large CFS units is the mechanism for generating collagen fibers. Is intrinsic tendon repair through the reestablishment of tendon embryogenesis? Gentamicin treated 10-day-old chick embryo tendons released CFS were fluorescently tagged with Rhodamine (Rh). Organ cultured severed 14-day-old embryo tendon explants... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5169354 Sat, 06 Aug 2011 23:00:00 +0100 5169354 http://www.medworm.com/index.php?rid=5146379&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840308%26dopt%3DAbstract Authors: Liu T, Ye L, Chen X, Peng J, Zhang X, Yi H, Peng F, He Y, Leng A Abstract Clinical trials of suicide gene therapy have achieved limited success, which suggests a need for improvement. Angiogenesis plays a crucial role in the progression of cancers, which is greatly regulated by vascular endothelial growth factor (VEGF).The current study was designed to evaluate the anti-tumor effects of VEGF siRNA in combination with fusion suicide gene yCDglyTK. Introduction of a VEGF-targeted small hairpin RNA (shVEGF) to CDTK/5-FC system could induce cell apoptosis more effectively and decrease micro vessel density in xenograft tissue, thus resulted in a significant tumor growth delay in SGC7901 xenografts. These findings for the first time suggest the potential of combination gene ther... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5146379 Sat, 06 Aug 2011 23:00:00 +0100 5146379 http://www.medworm.com/index.php?rid=5146380&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840307%26dopt%3DAbstract Authors: Schnabl B, Valletta D, Kirovski G, Hellerbrand C Abstract Zinc finger protein 267 (ZNF267) belongs to the family of Kruppel-like transcription factors, which regulates diverse biological processes that include development, proliferation, and differentiation. We have previously demonstrated that ZNF267 mRNA is up-regulated in liver cirrhosis, which is the main risk factor for hepatocellular carcinoma (HCC). Here, we analyzed the expression of ZNF267 in human HCC cells and tissue specimens and found a significant up-regulation compared to primary human hepatocytes and corresponding non-tumorous liver tissue. Over-expression of the transcription factor Ets-1 further enhanced ZNF267 expression, and reporter gene assays revealed that mutation of the Ets-1 binding site to the ZN... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5146380 Thu, 04 Aug 2011 23:00:00 +0100 5146380 http://www.medworm.com/index.php?rid=5146381&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21839737%26dopt%3DAbstract Authors: Bauer S, Weiss TS, Wiest R, Schacherer D, Hellerbrand C, Farkas S, Scherer MN, Ritter M, Schmitz G, Schäffler A, Buechler C Abstract Visceral fat differs from subcutaneous fat by higher local inflammation and increased release of IL-6 and free fatty acids (FFA) which contribute to hepatic steatosis. IL-6 has been shown to upregulate the monocyte/macrophage specific receptor CD163 whose soluble form, sCD163, is increased in inflammatory diseases. Here, it was analyzed whether CD163 and sCD163 are differentially expressed in the human fat depots and fatty liver. CD163 mRNA and protein were similarly expressed in paired samples of human visceral and subcutaneous fat, and comparable levels in portal venous and systemic venous blood of liver-healthy controls indicate that rele... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5146381 Tue, 02 Aug 2011 23:00:00 +0100 5146381 http://www.medworm.com/index.php?rid=5127020&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21821020%26dopt%3DAbstract Authors: Lazarin MD, Ishii-Iwamoto EL, Yamamoto NS, Constantin RP, Garcia RF, da Costa CE, Vitoriano AD, de Oliveira MC, Salgueiro-Pagadigorria CL The purpose of this work was to determine if mitochondrial dysfunction is involved in the development of non-alcoholic fatty liver disease (NAFLD). Using a model of obesity induced by the neonatal treatment of rats with monosodium l-glutamate (MSG), several parameters of liver mitochondrial function and their impact on liver redox status were evaluated. Specifically, fatty acid β-oxidation, oxidative phosphorylation and Ca(2+)-induced mitochondrial permeability transition were assessed in isolated liver mitochondria, and reduced glutathione (GSH), linked thiol contents and the activities of several enzymes involved in the control of redox s... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5127020 Thu, 28 Jul 2011 23:00:00 +0100 5127020 http://www.medworm.com/index.php?rid=5127022&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21820428%26dopt%3DAbstract Authors: French SW, Bardag-Gorce F, French BA, Li J, Oliva J Innate immunity factors such as conversion of the 26S proteasome to form the immunoproteasome and the Toll-like receptor signaling pathways are activated in chronic hepatitis induced by the carcinogenic drug DDC. Over time, preneoplastic hepatocyte phenotypes appear in the liver parenchyma. These changed hepatocytes expand in number because they have a growth advantage over normal hepatocytes when responding to chronic liver injury. The changed hepatocytes can be identified using immunofluorescent antibodies to preneoplastic cells e.g. FAT10/UbD, A2 macroglobulin, glutathione transpeptidase, alpha fetoprotein, glycipan 3, FAS, and gamma glutamyl transpeptidase. The formation of the preneoplastic cells occurs concomitant with ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5127022 Wed, 27 Jul 2011 23:00:00 +0100 5127022 http://www.medworm.com/index.php?rid=5078842&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798260%26dopt%3DAbstract CONCLUSIONS: Optimal IH with preservation of histological boundaries is achieved by graded BI limited to the CCA that preserves carotid blood flow. PMID: 21798260 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078842 Sun, 24 Jul 2011 23:00:00 +0100 5078842 http://www.medworm.com/index.php?rid=5078849&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21791203%26dopt%3DAbstract Authors: Tuszynski GP, Rothman VL, Zheng X, Gutu M, Zhang X, Chang F An innovative "2-D high performance liquid electrophoresis" (2-D HPLE) technology was used to identify serum biomarkers associated with the early stage of breast cancer in addition to other more advanced stages. 2-D HPLE is a newly developed electrophoretic technology that separates 100s of serum albumin complexes on a polyvinyl membrane based on their surface charges. Association of cancer proteins or their fragments (biomarkers) with pre-existing albumin complexes in the blood of cancer patients results in altered mobility on the membrane. Using 2-D HPLE we identified that a specific fragment of G-protein coupled receptor-associated sorting protein 1 (GASP-1) was present in the sera of patients with early stage dise... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078849 Fri, 22 Jul 2011 23:00:00 +0100 5078849 http://www.medworm.com/index.php?rid=5078847&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21791205%26dopt%3DAbstract This study was designed to investigate the role of α-galactosylceramide (α-GalCer) on experimental autoimmune myocarditis (EAM), and to explore the underlying mechanisms. Balb/c mice were immunized with porcine cardiac myosin to establish the EAM model. All the immunized mice were divided into two groups, the α-GalCer group and the EAM group. α-GalCer or vehicle was given intraperitoneally at the time of immunization. Then α-GalCer or PBS was injected on alternate days for 6weeks. Myocardial inflammation was evaluated by H & E staining and the expression levels of C/EBPβ and α-SMA were determined by immunohistochemistry. CD4(+)CD25(+)Foxp3(+) Tregs and iNKT cells were analyzed and sorted by flow cytometry. Western blot analysis was performed to detect MMP-2 and MMP-9 protein exp... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078847 Fri, 22 Jul 2011 23:00:00 +0100 5078847 http://www.medworm.com/index.php?rid=5078846&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798256%26dopt%3DAbstract Authors: McNally AK, Anderson JM Foreign body-type multinucleated giant cells (FBGC), formed by macrophage fusion, are a prominent cell type on implanted biomaterials, although the roles they play at these and other sites of chronic inflammation are not understood. Why lymphocytes are present in this scenario and the effects of fusing macrophages/FBGC on subsequent lymphocyte responses are also unclear. To address the physiological significance of FBGC in this regard, we employed our in vitro system of interleukin (IL)-4-induced human monocyte-derived macrophage fusion/FBGC formation. Initially, we pursued the identities of lymphocyte co-stimulatory molecules on fusing macrophages/FBGC. In addition, we further compared the FBGC phenotype to that currently associated with osteoclasts an... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078846 Fri, 22 Jul 2011 23:00:00 +0100 5078846 http://www.medworm.com/index.php?rid=5078845&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798257%26dopt%3DAbstract CONCLUSION: We can state that the increase of the activity of class III alcohol dehydrogenase isoenzyme in the sera of pancreatitis patients seems to be caused by the release of this isoenzyme from damaged pancreatic cells. PMID: 21798257 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078845 Fri, 22 Jul 2011 23:00:00 +0100 5078845 http://www.medworm.com/index.php?rid=5078844&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798258%26dopt%3DAbstract Authors: Kaneko Y, Obata Y, Nishino T, Kakeya H, Miyazaki Y, Hayasaka T, Setou M, Furusu A, Kohno S Immunoglobulin A (IgA) nephropathy is the most common glomerular disease worldwide. To investigate the pathogenesis of this renal disease, we used animal models that spontaneously develop mesangioproliferative lesions with IgA deposition, which closely resemble the disease in humans. We analyzed the molecular distribution of lipids in hyper-IgA (HIGA) murine kidneys using matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight (MALDI-QIT-TOF)-based imaging mass spectrometry (IMS), which supplies both spatial distribution of the detected molecules and allows identification of their structures by their molecular mass signature. For both HIGA and control (Balb/c) mice... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078844 Fri, 22 Jul 2011 23:00:00 +0100 5078844 http://www.medworm.com/index.php?rid=5078843&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798259%26dopt%3DAbstract This study focused on WT1 exons 7-10 mutations and their correlation with other molecular markers and patients' characteristics. The zinc finger domain of WT1 gene covering exons 7-10 was directly sequenced. Six types of mutations were identified among 49 cases (12.24%); 4 localized on exon 7 and 2 on exon 9. Two novel mutations were identified including the insertion within codon 313 and codon 314. Patients harboring WT1 mutations seemed to have a younger age (29.5 vs 45.4years), a higher white blood cell count (120.3 vs 19.8×10(9)/L), and a lower platelet count (54.2 vs 104.3×10(9)/L) as compared to those without the mutations although statistical differences could not be demonstrated. All exon 7 mutations were frameshift mutations and had NRAS mutation while exon 9 mutations were base... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078843 Fri, 22 Jul 2011 23:00:00 +0100 5078843 http://www.medworm.com/index.php?rid=5078848&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21791204%26dopt%3DAbstract Authors: Wanninger J, Walter R, Bauer S, Eisinger K, Schäffler A, Dorn C, Weiss TS, Hellerbrand C, Buechler C Adiponectin protects from inflammation and fibrosis in metabolic liver disease. In the present study we analyzed whether this adipokine may directly affect the activity of matrix metalloproteinases (MMPs), central regulators of fibrinolysis, in hepatocytes. Global gene expression analysis indicated upregulation of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1) expression in primary human hepatocytes (PHH) in response to stimulation with adiponectin, and these results were confirmed by real-time RT-PCR. Furthermore, gelatin zymography revealed that MMP-9 activity was significantly induced in supernatants of adiponectin stimulated PHHs. In a murine model of hepatic ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078848 Wed, 20 Jul 2011 23:00:00 +0100 5078848 http://www.medworm.com/index.php?rid=5078850&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21787767%26dopt%3DAbstract In conclusion, our study demonstrates that human ovarian cancer cells with the CD117(+) phenotype possess the unique properties of CSCs, including self-renewal, differentiation, a high tumorigenic potential, and chemoresistance. Future studies designed to target CD117(+) cancer cells may identify more attractive and effective therapies for treatment of ovarian cancer. PMID: 21787767 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078850 Tue, 19 Jul 2011 23:00:00 +0100 5078850 http://www.medworm.com/index.php?rid=5078853&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21777578%26dopt%3DAbstract CONCLUSIONS: Our results suggest that changes of these signals have effect on breaking the balance of proliferation and death of gallbladder epithelial cells, even on inducing gallbladder cancer. PMID: 21777578 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078853 Mon, 11 Jul 2011 23:00:00 +0100 5078853 http://www.medworm.com/index.php?rid=5078852&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21777579%26dopt%3DAbstract CONCLUSIONS: The present study suggests MS-LOH is a simple and specific assay for routinely diagnostic pathology. We recommend that D4S415, D1S507, D9S1752, D17S960, D17S1796 and D9S1749 can be used as the first-line markers for differential diagnosis between HGDN and w-MHCC, and D9S1748, D17S921 and D17S520 with a LOH frequency of 40%-50% in w-MHCC, but netative in HGDN, can be regarded as the second-line candidate markers. PMID: 21777579 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078852 Mon, 11 Jul 2011 23:00:00 +0100 5078852 http://www.medworm.com/index.php?rid=5078851&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21777580%26dopt%3DAbstract Authors: de A Simão T, Souza-Santos PT, de Oliveira DS, Bernardo V, Lima SC, Rapozo DC, Kruel CD, Faria PA, Ribeiro Pinto LF, Albano RM Esophageal squamous cell carcinoma (ESCC) is highly fatal due to late diagnosis and inefficient treatment. Early disease detection could improve diagnosis and patient survival. Esophageal squamous epithelial cells express SPRR3, a member of the small proline-rich protein family, which is downregulated in ESCC. Therefore, SPRR3 expression may be used as a biomarker to follow the transition from healthy mucosa to ESCC. Both SPRR3 mRNA splice variants, v1 and v2, were evaluated by real time PCR in tumor and histologically normal adjacent tissue biopsies from 84 ESCC patients and 18 healthy controls. SPRR3-v1 was most highly expressed in the esophageal mu... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078851 Mon, 11 Jul 2011 23:00:00 +0100 5078851 http://www.medworm.com/index.php?rid=5078854&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21763306%26dopt%3DAbstract CONCLUSIONS: These results suggested that SLC22A17, in cooperation with LCN2, to be involved in the acquisition of aggressive behavior among endometrial carcinoma cells. PMID: 21763306 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=5078854 Thu, 07 Jul 2011 23:00:00 +0100 5078854 http://www.medworm.com/index.php?rid=4984901&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21708146%26dopt%3DAbstract CONCLUSION: Betaine feeding lowers the BAL and prevents the BAL cycle by increasing the metabolic rate. This increases the rate of ethanol elimination by generating NAD. PMID: 21708146 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4984901 Thu, 23 Jun 2011 23:00:00 +0100 4984901 http://www.medworm.com/index.php?rid=4984902&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21689646%26dopt%3DAbstract The objective of the present study was to assess the immunolocalization of aggrecan in the annulus, and to assess molecular gene expression patterns in the annulus ECM utilizing microarray analysis. Immunohistochemistry was performed on 45 specimens using an anti-human aggrecan antibody. Affymetrix microarray gene expression studies used the extracellular matrix ontology approach to evaluate an additional 6 grade I-II, 9 grade III, and 4 grade IV disks. Grade III/IV disks were compared to healthier grade I/II disks. Healthy and less degenerated disks showed a general uniform aggrecan immunolocalization; more degenerated disks contained regions with little or no identifiable aggrecan localization. In degenerated disks, molecular studies showed a significant downregulation of aggrecan, ADAMT... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4984902 Sat, 11 Jun 2011 23:00:00 +0100 4984902 http://www.medworm.com/index.php?rid=4931804&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21672537%26dopt%3DAbstract Authors: Joseph LB, Gerecke DR, Heck DE, Black AT, Sinko PJ, Cervelli JA, Casillas RP, Babin MC, Laskin DL, Laskin JD Sulfur mustard (SM, bis(2-chloroethyl)sulfide) is a bifunctional alkylating agent that causes dermal inflammation, edema and blistering. To investigate the pathogenesis of SM-induced injury, we used a vapor cup model which provides an occlusive environment in which SM is in constant contact with the skin. The dorsal skin of SKH-1 hairless mice was exposed to saturated SM vapor or air control. Histopathological changes, inflammatory markers and DNA damage were analyzed 1-14days later. After 1day, SM caused epidermal thinning, stratum corneum shedding, basal cell karyolysis, hemorrhage and macrophage and neutrophil accumulation in the dermis. Cleaved caspase-3 and phospho... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931804 Fri, 10 Jun 2011 23:00:00 +0100 4931804 http://www.medworm.com/index.php?rid=4931807&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669196%26dopt%3DAbstract We present a case of IMT of the lung in a 58year old female patient with a single lung nodule. Tumor was unencapsulated, firm, and well circumscribed. Microscopically tumor had multinodular structure with single or multiple small blood vessels in the center of each nodule surrounded in circular pattern by connective tissue containing spindle cells embedded into the thick layers of extracellular matrix. Extracellular matrix was identified as type I and type III collagen fibrils embedded into type IV collagen and laminin. The tumor was surrounded by T-, B-lymphocytes and polyclonal plasma cells. Histological organization of this lesion's stromal component was unique, but cell composition was similar to inflammatory pseudotumor of the lung. In addition, tumor tissue sections exhibited strong ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931807 Sun, 05 Jun 2011 23:00:00 +0100 4931807 http://www.medworm.com/index.php?rid=4931811&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21641900%26dopt%3DAbstract Authors: Arzt L, Kothmaier H, Quehenberger F, Halbwedl I, Wagner K, Maierhofer T, Popper HH FineFix, RCL-2 and HOPE, three formalin-free fixatives, were compared to the common used formalin fixed tissue samples of lung cancer and were evaluated for their effects on quality, quantity and integrity of RNA and microRNA. Two commercially available RNA extraction Kits (RNeasy FFPE by Qiagen and RecoverAll™ Nucleic Acid Isolation by Ambion) were tested and optimized in order to determine an extraction protocol for RNA as well as miRNA independent of the fixative. Two selected miRNAs were quantified via TaqMan MicroRNA assays. The optimized RNA extraction protocol for Qiagen's Kit leads to similar results for RNA quality and integrity for all fixatives. Highest RNA yield was obtained for fo... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931811 Sun, 29 May 2011 23:00:00 +0100 4931811 http://www.medworm.com/index.php?rid=4931829&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21640719%26dopt%3DAbstract In conclusion, HMGB1 translocation and expression depends on the type of liver injury as it is induced by ischemia, but not by liver resection/hyperperfusion. These results suggest that HMGB1 may be used as molecular marker to visualize ischemic damage. Mechanic injury in hepatic surgery is associated with focal warm ischemia, and thereby HMGB1 translocation reflects surgical quality in experimental PH. Expression of hepatic TNF-α follows the kinetic pattern of HMGB1, pointing to a muss less pronounced inflammatory response after successful PH compared to warm I/R injury. PMID: 21640719 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931829 Thu, 26 May 2011 23:00:00 +0100 4931829 http://www.medworm.com/index.php?rid=4931827&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21640720%26dopt%3DAbstract Authors: Bardag-Gorce F, French SW The proteasome interacts with a large number of proteins which regulate specific cellular functions. The focus of this study is to examine the proteasome interaction with Delta-aminolevulinate dehydratase (ALAD). ALAD is involved in the heme biosynthesis pathway and was co-isolated, with the 20S proteasome using several chromatographic purification steps. The MALDI-TOF mass spectrometry analysis identified this proteasome co-isolated protein as ALAD. When the proteasome was isolated using density-gradient centrifugation, ALAD was also found in the 26S proteasome fractions. It co-isolated with the 20S more than with the 26S proteasome. Furthermore, immunoprecipitated ALAD stained positive with antibodies to proteasome subunits. These results indicate t... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931827 Thu, 26 May 2011 23:00:00 +0100 4931827 http://www.medworm.com/index.php?rid=4931818&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21640721%26dopt%3DAbstract Authors: Aggarwal H, Aggarwal A, Agrawal DK Leukemia/lymphoma related factor/POK erythroid myeloid ontogenic factor (LRF/Pokemon) is a member of the POK family of proteins that promotes oncogenesis in several forms of cancer. Recently, we found higher LRF expression in human breast and prostate carcinomas compared to the corresponding normal tissues. The aim of this study was to examine the regulation of LRF expression in human prostate cells. Epidermal growth factor (EGF) and its receptors mediate several tumorigenic cascades that regulate cell differentiation, proliferation, migration and survival of prostate cancer cells. There was significantly higher level of LRF expression in the nucleus of LNCaP and PC-3 cells than RWPE-1 cells. A significant increase in LRF expression was obser... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931818 Thu, 26 May 2011 23:00:00 +0100 4931818 http://www.medworm.com/index.php?rid=4931814&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21640722%26dopt%3DAbstract Authors: Hentosh P, Benjamin T, Hall L, Leap S, Loescher J, Poyner E, Sundin T, Whittle M, Wilkinson S, Peffley DM Deficiencies of DNA polymerase eta-an enzyme mediating replication past UV-induced DNA damage-predispose individuals to xeroderma pigmentosum variant (XPV) and result in a high incidence of skin cancers. We designed, developed and assessed several complementary molecular approaches to detect a genetically inherited deletion within DNA polymerase eta. RNA was reverse transcribed from XPV fibroblasts and from normal human cells, and standard polymerase chain reaction (PCR) was conducted on the cDNA targeting a region with a 13 base pair deletion within the polymerase eta gene. PCR products were subjected to restriction fragment length polymorphism (RFLP) analysis and cycle D... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4931814 Thu, 26 May 2011 23:00:00 +0100 4931814 http://www.medworm.com/index.php?rid=4885243&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21600201%26dopt%3DAbstract This study was aimed at investigating the methylation levels of multiple genes in hepatocellular carcinoma (HCC) and to identify a combination of methylation markers that would be useful for the diagnosis of HCC. The methylation status of a panel of nine tumor-associated genes (APC, GSTP1, RASSF1A, CDKN2A, SFRP1, RUNX3, SOCS1, Hint1, and HIC-1) in 8 normal liver tissues and 47 paired HCCs and non-tumorous tissues (NTs) was determined using a modified methylation-sensitive, restriction enzyme-based quantitative PCR (MSRE-qPCR) method. The methylation levels of six genes (APC, CDKN2A, GSTP1, RASSF1A, SFRP1 and RUNX3) were significantly higher in HCCs than in adjacent NTs (P<0.05). Although the AUC (area under the curve) for each individual gene was low to moderate (range: 0.576 to 0.835) ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4885243 Tue, 10 May 2011 23:00:00 +0100 4885243 http://www.medworm.com/index.php?rid=4885245&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21596033%26dopt%3DAbstract Authors: Baños M, Arellano-Mendoza MG, Vargas-Robles H, Avila-Casado MC, Soto V, Romo E, Rios A, Hernandez-Zavala A, la Peña-Diaz AD, Escalante B The density of Angiotensin II (Ang) receptors on tissue surfaces is regulated by multiple hormones, cytokines and metabolic factors and is profoundly affected by various pathological conditions, such as age, diet and environmental conditions. The participation of several cardiovascular risk factors in the regulation of Angiotensin II receptor expression has been incompletely studied. We performed an ex-vivo study with human aortic postsurgical specimens to test the hypothesis that Ang AT1 and AT2 receptor expression in human aortic arteries is associated with the presence of cardiovascular risk factors. We included 31 Mexican patients with ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4885245 Sat, 07 May 2011 23:00:00 +0100 4885245 http://www.medworm.com/index.php?rid=4885246&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21586283%26dopt%3DAbstract Authors: Bai Y, Bai X, Wang Z, Zhang X, Ruan C, Miao J To investigate the potential transcriptional regulation and signal pathway of a single microRNA in ischemia-induced retinal neovascularization (NV), we used oxygen-induced retinopathy (OIR) in establishing retinal NV model, and quantitative real-time reverse transcriptase PCR analyzing a microRNA (mir-126) alteration. The mice were treated with plasmid pCMV-MIR-126/liposome mixture intravitreal injection, using pCMV-MIR/liposome mixture as control. The expression levels of VEGF, IGF-2 and HIF-1α, and the level changes of total and phosphorylated p38, ERK in retina from OIR mice were determined by western blot analysis. The effects of miR-126 on retinal NV in OIR mice were identified with fluoresecin angiography and H & E stain... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4885246 Thu, 05 May 2011 23:00:00 +0100 4885246 http://www.medworm.com/index.php?rid=4885244&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21596034%26dopt%3DAbstract CONCLUSION: Compared with normal tissues, the levels of DC-LAMP and DC-SIGN were higher in psoriasis vulgaris lesions. Both DC-LAMP and DC-SIGN proteins may be involved in the pathogenesis of psoriasis vulgaris. The high expression of DC-LAMP may be associated with the altered differentiation of keratynocytes in psoriasis. PMID: 21596034 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4885244 Thu, 05 May 2011 23:00:00 +0100 4885244 http://www.medworm.com/index.php?rid=4885242&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21600202%26dopt%3DAbstract Authors: Khan G, Philip PS, Al Ashari M, Houcinat Y, Daoud S The pathogenesis of breast cancer is unknown. In recent years, a number of studies have implicated a role for Epstein-Barr virus (EBV) in a subset of cases. However, these findings are controversial and others have failed to find any link between the virus and this malignancy. We hypothesized that technical differences and the different type and ethnic origin of the cases may be the cause of the disparities reported. Using a highly sensitive EBER-in situ hybridization and immunohistochemistry, we examined 219 samples (158 malignant and 61 non-malignant) from 61 Emirati breast cancer cases to determine if EBV was etiologically associated with Emirati cases and if there was any correlation with other established prognostic fact... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4885242 Thu, 05 May 2011 23:00:00 +0100 4885242 http://www.medworm.com/index.php?rid=4833853&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21569770%26dopt%3DAbstract CONCLUSION: IDH1 mutations are present in large proportion of Indian patients with diffuse astrocytic and oligodendroglial neoplasms similar to the reported literature form west. The frequency is lower in primary GBMs and as compared to secondary GBMs. Association with younger age and positive correlation with T p53 mutation and 1p/19q loss is observed. More importantly it is emerging as an independent prognostic marker. Hence the greatest challenge now is establishing a reliable user friendly test for incorporating this novel genetic alteration to routine clinical practice. PMID: 21569770 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833853 Mon, 02 May 2011 23:00:00 +0100 4833853 http://www.medworm.com/index.php?rid=4833852&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21570964%26dopt%3DAbstract Authors: Sinha-Hikim I, Sinha-Hikim AP, Shen R, Kim H, French SW, Vazari ND, Crum A, Rajavashisth TB, Norris KC Nonalcoholic fatty liver disease (NAFLD) is the most common form of liver pathologies and is associated with obesity and the metabolic syndrome. Here, we investigated the molecular mechanisms by which a novel cystine based glutathione precursor with added selenomethionine (F1) prevents hepatic steatosis in a moderate high fat dietary model of NAFLD. Adult (8weeks old), male apolipoprotein E (ApoE)-/- mice were fed with a normal diet (ND) or high fat diet (HFD), consisting of 21% fat and 0.21% cholesterol, with or without dietary supplementation of F1 (3g/kg food) for 16weeks. Compared with ApoE-/- mice fed with ND with or without F1, ApoE-/- mice fed with HFD exhibited signif... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833852 Mon, 02 May 2011 23:00:00 +0100 4833852 http://www.medworm.com/index.php?rid=4833854&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565186%26dopt%3DAbstract In conclusion, these data indicate that much caution should be taken when using GAPDH as a RNA reference gene and 28S rRNA is an excellent gene for the RNA study in p53-deficient mice with UUO. PMID: 21565186 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833854 Sun, 01 May 2011 23:00:00 +0100 4833854 http://www.medworm.com/index.php?rid=4833858&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565182%26dopt%3DAbstract In this report, we hypothesized that BST2 is expressed in human and mouse brain tumors and plays a critical role in brain tumor progression. We show that BST2 expression is upregulated at both the mRNA and protein level in high grade when compared to low grade human astrocytoma (p<0.05). To test the relevance of BST2, we utilized the intracranially (IC)-injected GL261 cell-based malignant brain tumor mouse model. We show that BST2 mRNA expression is increased in mouse brain IC-injected GL261 cells, when compared to mouse brain IC-injected saline at 3weeks post-operative (p<0.05). Furthermore, BST2 immunofluorescence predominantly localized to mouse brain tumor cells. Finally, mice IC-injected with GL261 cells transduced with shRNA for BST2±pre-incubation with BST2 mAb show no differ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833858 Sat, 30 Apr 2011 23:00:00 +0100 4833858 http://www.medworm.com/index.php?rid=4833857&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565183%26dopt%3DAbstract In conclusion, quantitative assessment of heteroduplex products from single-tube PCR/DHPLC is simple and rapid, and can be used to detect α-thalassemia gene deletions (α(-3.7), α(-4.2)) and trisomy-21. PMID: 21565183 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833857 Sat, 30 Apr 2011 23:00:00 +0100 4833857 http://www.medworm.com/index.php?rid=4833856&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565184%26dopt%3DAbstract The objective was to employ small molecule inhibitors to target several key steps in the autocrine loop, and in this way ameliorate pathology. Our results indicate that upregulation of ERK phosphorylation is necessary for initial mCMV-induced pathogenesis, and that ErbB receptor family phosphorylation and downstream signaling are highly relevant targets for drug discovery. PMID: 21565184 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833856 Sat, 30 Apr 2011 23:00:00 +0100 4833856 http://www.medworm.com/index.php?rid=4833855&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565185%26dopt%3DAbstract CONCLUSION: It demonstrated that hyperhomocysteinemia may significantly contribute to restrain CSCs-mediated cardiac repair by reducing SCF-induced homing of CSCs. PMID: 21565185 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833855 Sat, 30 Apr 2011 23:00:00 +0100 4833855 http://www.medworm.com/index.php?rid=4833859&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21545801%26dopt%3DAbstract In this study, the influence of E(2) overdose on the number and distribution of neurons in the sympathetic chain ganglia (SChG) projecting to the ovary of adult pigs was investigated. The numbers of ovarian dopamine-β-hydroxylase (DβH-), neuropeptide Y (NPY-), somatostatin (SOM-), galanin (GAL-) and estrogen receptors (ERs-) immunoreactive perikarya as well as the density of the intraganglionic nerve fibers containing DβH and/or NPY, SOM, GAL were also determined. On day 3 of the estrous cycle the ovaries of both the control and experimental gilts were injected with retrograde neuronal tracer Fast Blue, to identify the neurons innervating gonads. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with E(2), while the ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833859 Tue, 26 Apr 2011 23:00:00 +0100 4833859 http://www.medworm.com/index.php?rid=4833862&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21540025%26dopt%3DAbstract Authors: Tegla CA, Cudrici C, Rozycka M, Soloviova K, Ito T, Singh AK, Khan A, Azimzadeh P, Andrian-Albescu M, Khan A, Niculescu F, Rus V, Judge SI, Rus H Voltage-gated potassium (K(v)) channels play an important role in the regulation of growth factor-induced cell proliferation. We have previously shown that cell cycle activation is induced in oligodendrocytes (OLGs) by complement C5b-9, but the role of K(v) channels in these cells had not been investigated. Differentiated OLGs were found to express K(v)1.4 channels, but little K(v)1.3. Exposure of OLGs to C5b-9 modulated K(v)1.3 functional channels and increased protein expression, whereas C5b6 had no effect. Pretreatment with the recombinant scorpion toxin rOsK-1, a highly selective K(v)1.3 inhibitor, blocked the expression of K(v)1... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833862 Thu, 21 Apr 2011 23:00:00 +0100 4833862 http://www.medworm.com/index.php?rid=4833861&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21540026%26dopt%3DAbstract CONCLUSIONS: Serous adenocarcinoma exhibits distinct gene expression profiles, compared with those of endometrioid adenocarcinoma. These differences make it feasible to validate microarray data by immunohistochemistry, and they will ultimately allow us to identify tumors according to their immunohistochemical phenotype. The accuracy of classifying endometrial tumors using a system based on their gene expression patterns is much higher than the accuracy of the FIGO grading system. Thus, this gene expression pattern-based system may prove to be crucial in developing novel treatment strategies for endometrial cancers at the molecular level in future. PMID: 21540026 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833861 Thu, 21 Apr 2011 23:00:00 +0100 4833861 http://www.medworm.com/index.php?rid=4833860&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21540027%26dopt%3DAbstract CONCLUSIONS: These data suggest that SOCS-3 expression is decreased in proliferating smooth muscle cells of neointimal lesions. This leads to uncontrolled growth of vascular smooth muscle cells in injured arteries leading to restenosis. Therefore, local delivery of SOCS-3 gene at the site of injury after coronary artery intervention could regulate the proliferation of vascular smooth muscle cells and help in preventing the neointimal hyperplasia and restenosis. PMID: 21540027 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4833860 Thu, 21 Apr 2011 23:00:00 +0100 4833860 http://www.medworm.com/index.php?rid=4781078&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21530503%26dopt%3DAbstract Authors: Homcha C, Ehrlich HP Open wound contraction necessitates cell and connective tissue interactions, that produce tension. Investigating fibroblast responses to tension utilizes collagen coated polyacrylamide gels with differences in stiffness. Human foreskin fibroblasts were plated on native type I collagen-coated polyacrylamide gel cover slips with different rigidities, which were controlled by bis-acrylamide concentrations. Changes in alpha smooth muscle actin (αSMA), α2β1 integrin (CD49B) and αvβ3 integrin (CD-51) were documented by immuno-histology and Western blot analysis. Cells plated on rigid gels were longer, and expressed αvβ3 integrin and αSMA within cytoplasmic stress fibers. In contrast, cells on flexible gels were shorter, expressed α2β1 integrin and had ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781078 Thu, 21 Apr 2011 23:00:00 +0100 4781078 http://www.medworm.com/index.php?rid=4781076&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21536027%26dopt%3DAbstract Authors: Zargan J, Sajad M, Umar S, Naime M, Ali S, Khan HA The purpose of study was to examine the cytotoxic and anti-cancer properties along with addressing the plausible pathway followed by scorpion venom to reduce cell viability in SH-SY5Y and MCF-7 cells. Following exposure of cells with scorpion venom, cytotoxicity was estimated using MTT and lactate dehydrogenase assays. Apoptotic effects were measured by assessment of mitochondrial membrane potential, reactive nitrogen species, DNA fragmentation, and caspase-3 activity whereas antiproliferative effect was assayed using BrdU incorporation. Our results indicate that scorpion venom causes suppression of proliferation by arresting S-phase and induction of apoptosis through increased nitric oxide production, caspase-3 activity and d... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781076 Thu, 21 Apr 2011 23:00:00 +0100 4781076 http://www.medworm.com/index.php?rid=4781077&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21530504%26dopt%3DAbstract CONCLUSIONS: IL-17/IL-17RA signaling plays an important role in myocardial collagen metabolism in hypertension-induced diastolic dysfunction. PMID: 21530504 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781077 Wed, 20 Apr 2011 23:00:00 +0100 4781077 http://www.medworm.com/index.php?rid=4781079&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21510931%26dopt%3DAbstract Cytokeratin 8/18 monoclonal antibody was dissimilar to anti-cytokeratin CAM 5.2. - A comment on: "Discovery of two novel EWSR1/ATF1 transcripts in four chimerical transcripts-expressing clear cell sarcoma and their quantitative evaluation, Experimental and Molecular Pathology 90(2): 194-200, April 2011" Exp Mol Pathol. 2011 Apr 13; Authors: Lin WL, Chen FL, Kuo JF, Lee MY, Han CP PMID: 21510931 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781079 Tue, 12 Apr 2011 23:00:00 +0100 4781079 http://www.medworm.com/index.php?rid=4781081&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21497600%26dopt%3DAbstract CONCLUSIONS: Despite the use of a high-density SNP platform, our results suggest that genome-wide LOH and copy number amplifications are infrequent events and generally do not determine clinical and histologic characteristics of this disease. PMID: 21497600 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781081 Thu, 07 Apr 2011 23:00:00 +0100 4781081 http://www.medworm.com/index.php?rid=4781080&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21497601%26dopt%3DAbstract This study aims to characterize the immune cell population in patients from our community suffering from systemic lupus erythematosus and to prove that these patients exhibit a unique cellular profile compared to healthy age, race and gender matched control subjects. Surprisingly, our findings demonstrate that patients from the local Mississippi area exhibit increased proportions of CD25(+) FoxP3(+) regulatory T cells and CD25(+) FoxP3(-) T cells (of CD45(+) CD3(+) CD4(+) helper T cells) as compared to healthy controls. HLA tissue-typing of these lupus patients revealed a prominent subgroup (~30%) of patients possessing the HLA DRB1*1503 allele. The investigation of this subgroup demonstrated regulatory T cell composition similar to that of the total lupus group and to that of the non-HLA ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781080 Thu, 07 Apr 2011 23:00:00 +0100 4781080 http://www.medworm.com/index.php?rid=4781082&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21463625%26dopt%3DAbstract Authors: Wang YT, Li W, Liu Q, Guan X, Hu J Although the human papillomavirus (HPV) DNA therapeutic vaccine represents a promising approach to the prevention and treatment of cervical cancer, the mechanism of the HPV DNA vaccine is poorly understood. Moreover, current strategies have met with only limited success in preclinical and dendritic cell-based (DC-based) clinical research. In addition, two-dimensional (2-D) DC monolayers poorly mimic the physiology function in vivo. We used a three-dimensional (3-D) DC culture model in vitro to explore the immune mechanism of the HPV DNA vaccine. DCs were generated from peripheral blood monocytes with interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF). The cells, growing in 3-D collagen gel, were treated with pc... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4781082 Fri, 01 Apr 2011 23:00:00 +0100 4781082 http://www.medworm.com/index.php?rid=4676432&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21419115%26dopt%3DAbstract CONCLUSION: Carriers of the homozygous ERCC2 751 Gln/Gln genotype may be at lower risk for HNSCC. PMID: 21419115 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4676432 Tue, 22 Mar 2011 00:00:00 +0100 4676432 http://www.medworm.com/index.php?rid=4615797&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397596%26dopt%3DAbstract Authors: Wang F, Zou YF, Feng XL PMID: 21397596 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4615797 Thu, 10 Mar 2011 00:00:00 +0100 4615797 http://www.medworm.com/index.php?rid=4615796&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397597%26dopt%3DAbstract "Polymorphisms of the XRCC3 C722T and the RAD51 G135C genes and the risk of head and neck cancer in a Polish population" by Sliwinski et al. published in Experimental and Molecular Pathology, 2010 Dec;89(3):358-66. Exp Mol Pathol. 2011 Mar 10; Authors: Majsterek I PMID: 21397597 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4615796 Thu, 10 Mar 2011 00:00:00 +0100 4615796 http://www.medworm.com/index.php?rid=4615798&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21385580%26dopt%3DAbstract In conclusion, ATRA can regulate the expression of apoE, reduce the accumulation of extracellular matrix (ECM) and step down the progression of GS. PMID: 21385580 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4615798 Sat, 05 Mar 2011 00:00:00 +0100 4615798 http://www.medworm.com/index.php?rid=4615799&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21376035%26dopt%3DAbstract In this study, the entire human genome was subjected to an unbiased RE survey to establish a whole-genome profile of REs and their arrangements. Due to the limitation in query size within the bl2seq alignment program (National Center for Biotechnology Information [NCBI]) utilized for the RE survey, the entire NCBI reference human genome was fragmented into 6206 units of 0.5M nucleotides. A number of RE arrangements with varying complexities and patterns were identified throughout the genome. Each chromosome had unique profiles of RE arrangements and density, and high levels of RE density were measured near the centromere regions. Subsequently, 175 complex RE arrangements, which were selected throughout the genome, were subjected to a comparison analysis using five different human genome se... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4615799 Tue, 01 Mar 2011 00:00:00 +0100 4615799 http://www.medworm.com/index.php?rid=4555064&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21352821%26dopt%3DAbstract In conclusion, the in vitro model of oxidative stress induced by ethanol provided evidence that all 4 agents tested prevented some aspect of liver cell injury caused by ethanol. PMID: 21352821 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4555064 Wed, 23 Feb 2011 00:00:00 +0100 4555064 http://www.medworm.com/index.php?rid=4555063&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21352822%26dopt%3DAbstract CONCLUSION: Increased expression of NPY-Y2 receptors in symptomatic pVSMCs than in healthy and asymptomatic subjects suggests a potential role of NPY-Y2 in plaque instability. This is further supported by the pronounced effect of atheroma-associated cytokines to increase NPY-Y2 mRNA transcripts in pVSMCs of patients with carotid stenosis. PMID: 21352822 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4555063 Tue, 22 Feb 2011 00:00:00 +0100 4555063 http://www.medworm.com/index.php?rid=4555066&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21333644%26dopt%3DAbstract Authors: Sediri Y, Hammami S, Kallel A, Mourali MS, Feki M, Elasmi M, Haj-Taieb S, Omar S, Sanhaji H, Mechmeche R, Jemaa R, Kaabachi N Recent findings suggest that inflammation plays a role in atherosclerosis and its acute complications. Several known mechanisms may play at least a partial role in this process. One of the most likely mechanisms involves lipopolysaccharide (LPS) and its receptor, CD14. The C(-260)T single nucleotide polymorphism (rs2569190) in the promoter region of the CD14 receptor gene has been reported to be associated with a higher risk of MI. Others studies, however, have not corroborated these findings. Considering the contradictory results, the aim of the present study was to investigate the possible association between the CD14 C(-260)T polymorphism and the ris... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4555066 Thu, 17 Feb 2011 00:00:00 +0100 4555066 http://www.medworm.com/index.php?rid=4555065&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21335003%26dopt%3DAbstract In this study, we aimed to compare the expressions of COX-2, E-cadherin, VEGF to classical prognostic factors, and to investigate the correlation with prognosis. Operation records from 79 PTC cases were examined retrospectively. In the study, we aimed to investigate the whole tissue by means of stereology method, which is an impartial one, and we indicated the expression COX-2, VEGF, E-cadherin immunohistochemically in 79 resection diagnosed with PTC. We determined correlations between the expressions of COX-2 and VEGF, E-cadherin, and age, gender, and stage. PMID: 21335003 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4555065 Wed, 16 Feb 2011 00:00:00 +0100 4555065 http://www.medworm.com/index.php?rid=4500561&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21324312%26dopt%3DAbstract Authors: Nemoto H, Konno S, Sugimoto H, Nakazora H, Nomoto N, Murata M, Kitazono H, Fujioka T Limb-girdle muscular dystrophy 2B and Miyoshi myopathy are characterized by muscle fiber necrosis caused by a defect in dysferlin and inflammatory changes. SJL/J mice are deficient in dysferlin and display severe inflammatory changes, most notably the presence of cytokines, which may be related to destruction of the sarcolemma. We tested the hypothesis that tumor necrosis factor (TNF) contributes to myofibril necrosis. Administration of etanercept, an agent that blocks TNF, resulted in dose-dependent reductions in inflammatory change, necrosis, and fatty/fibrous change. These findings indicate that TNF does indeed play a role in damage to muscle in SJL/J mice and that etanercept has the potent... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4500561 Sat, 12 Feb 2011 00:00:00 +0100 4500561 http://www.medworm.com/index.php?rid=4500562&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316360%26dopt%3DAbstract Authors: PMID: 21316360 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4500562 Thu, 10 Feb 2011 00:00:00 +0100 4500562 http://www.medworm.com/index.php?rid=4500564&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315713%26dopt%3DAbstract Authors: Gonçalves CF, Morais MO, Alencar RD, Mota ED, da Silva TA, Batista AC, Mendonça EF PMID: 21315713 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4500564 Wed, 09 Feb 2011 00:00:00 +0100 4500564 http://www.medworm.com/index.php?rid=4500563&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315714%26dopt%3DAbstract Authors: Bejar E, Smith CR, Appelhans K, Henig YS PMID: 21315714 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4500563 Wed, 09 Feb 2011 00:00:00 +0100 4500563 http://www.medworm.com/index.php?rid=4442311&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21281625%26dopt%3DAbstract CONCLUSIONS: Our study indicates that nutritionally relevant concentrations (nM) of genistein inhibit the proliferative effects of estrogen on endometrial adenocarcinoma cells presumably through activation of stromal cell ERβ. We believe that sub-micromolar concentrations of genistein may represent a novel adjuvant for endometrial cancer treatment and prevention. PMID: 21281625 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4442311 Fri, 28 Jan 2011 00:00:00 +0100 4442311 http://www.medworm.com/index.php?rid=4442312&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276439%26dopt%3DAbstract Authors: Oliva J, Bardag-Gorce F, Li J, French BA, French SW Toll-like receptors (TLR) play a role in mediating the proinflammatory response, fibrogenesis and carcinogenesis in chronic liver diseases such as alcoholic liver disease, non-alcoholic liver disease, hepatitis C and hepatocellular carcinoma. This is true in experimental models of these diseases. For this reason, we investigated the TLR proinflammatory response in the chronic intragastric tube feeding rat model of alcohol liver disease. The methyl donor S-adenosylmethionine was also fed to prevent the gene expression changes induced by ethanol. Ethanol feeding tended to increase the up regulation of the gene expression of TLR2 and TLR4. SAMe feeding prevented this. TLR4 and MyD88 protein levels were significantly increased by... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4442312 Tue, 25 Jan 2011 00:00:00 +0100 4442312 http://www.medworm.com/index.php?rid=4442313&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21256843%26dopt%3DAbstract Authors: French BA, Oliva J, Bardag-Gorce F, French SW Recently it has been shown that the expression of the immunoproteasome increased in proportion to the degree of chronic inflammation in both the liver cell cytoplasm and nuclei in liver biopsies from patients who had chronic active hepatitis or cirrhosis. In the present study, biopsies from patients with steatohepatitis, with or without Mallory-Denk body (MDB) formation, were studied by immunofluorescent staining. Normal liver showed colocalization of FAT10, LMP2, LMP7, and MECL-1 at the mitochondria. Only LMP2 and LMP7 were found in the cell nuclei. Liver biopsies from patients with steatohepatitis and MDB formation, and a case of hepatocellular carcinoma forming MDBs in the tumor cells, showed colocalization of FAT10 and ubiquiti... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4442313 Fri, 21 Jan 2011 00:00:00 +0100 4442313 http://www.medworm.com/index.php?rid=4442314&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21251909%26dopt%3DAbstract In this study, the expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. The functional expression of LRF was studied using multiple cellular and molecular methods including RT-PCR, western blotting, immunohistochemistry, and immunofluorescence. Paraffin-embedded human tissues of BPH and PC were used to examine LRF expression. Histological staining of the BPH and PC tissue sections revealed nuclear expression of LRF with minimal expression in the surrounding stroma. The semi-quantitative RT-PCR and western immunoblot analyses demonstrated significantly higher mRNA transcripts and protein expression in PC than BPH. High expression of LRF suggests that it may have a potential role in the pathogenesis of both BPH and prostate cancer. Further s... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4442314 Tue, 18 Jan 2011 00:00:00 +0100 4442314 http://www.medworm.com/index.php?rid=4378268&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21236253%26dopt%3DAbstract Authors: Vigneswaran N, Wu J, Song A, Annapragada A, Zacharias W Hypoxia confers resistance to chemoradiation therapy and promotes metastasis in head and neck squamous cell carcinomas (HNSCC). We investigated the effects of hypoxia in tumor phenotype using immunocompetent murine HNSCC models. Balb/c mice were injected intraorally with murine squamous cell carcinoma cells LY-2 and B4B8. Intratumoral hypoxia fraction was evaluated by the immunohistochemical detection of hypoxic probe pimonidazole and carbonic anhydrase IX (CAIX). Tumor cell apoptosis and autophagy in hypoxic areas of these tumors were examined immunohistochemically. Hypoxia-induced apoptotic and autophagic responses in vitro were examined by treating LY2 cells with CoCl(2). B4B8 tumors exhibited a non-aggressive phenotyp... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378268 Fri, 14 Jan 2011 00:00:00 +0100 4378268 http://www.medworm.com/index.php?rid=4378267&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241689%26dopt%3DAbstract In conclusion our data revealed that oxidative stress had a pathogenic role in primary open-angle glaucoma. Therefore, we suggested that the modulation of a pro-oxidant/antioxidant status might be a relevant target for glaucoma prevention and therapy. PMID: 21241689 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378267 Fri, 14 Jan 2011 00:00:00 +0100 4378267 http://www.medworm.com/index.php?rid=4378266&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241690%26dopt%3DAbstract Authors: Godek J, Sargiannidou I, Patel S, Hurd L, Rothman VL, Tuszynski GP Angiocidin, a tumor-associated peptide, has been previously shown to inhibit tumor progression by blocking angiogenesis. We now show that angiocidin has a direct inhibitory effect on tumor cell proliferation. MDA-MB-231 breast cancer cells were inhibited from proliferating in the presence of epidermal growth factor (EGF) and angiocidin. Angiocidin transfected breast cancer cells also displayed growth inhibition in vitro and failed to develop significant tumors in mice as compared to vector controls. The anti-proliferative effect of angiocidin was reversed by treating the cells with the epidermal growth factor receptor (EGFR) inhibitor 4557W, a potent tyrosine kinase inhibitor. Consistent with these results, we ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378266 Fri, 14 Jan 2011 00:00:00 +0100 4378266 http://www.medworm.com/index.php?rid=4378270&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21194529%26dopt%3DAbstract CONCLUSION: The present study showed a significant and independent association between the -786TC gene polymorphism (presence of C allele) and hypertension in the Tunisian population. PMID: 21194529 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378270 Wed, 29 Dec 2010 00:00:00 +0100 4378270 http://www.medworm.com/index.php?rid=4378269&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21194530%26dopt%3DAbstract CONCLUSIONS: Absence of galectin-3 did not directly affect the process of carcinogenesis and a cytoplasm shift of galectin-3 seems to be associated with development of tongue carcinomas. PMID: 21194530 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378269 Wed, 29 Dec 2010 00:00:00 +0100 4378269 http://www.medworm.com/index.php?rid=4378272&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21185829%26dopt%3DAbstract We examined gene expression changes during the gastric adenoma-carcinoma sequence in 26 snap-frozen samples (normal mucosa, adenoma, and carcinoma samples from eight patients and two additional carcinomas) by oligonucleotide microarray. Unsupervised hierarchical clustering analysis demonstrated differential gene expression between gastric normal mucosa, adenomas and carcinomas. We identified 319 and 422 genes differentially regulated in adenoma and carcinoma, respectively, relative to normal mucosa, using a combination of Welch's t-test and fold-change analysis. Applying a combination of robust multi-category support vector machines to the data, reveal that 39 and 21 genes were gradually up- and down-regulated, respectively, in succession in normal mucosa, adenoma, and carcinoma samples. W... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378272 Fri, 24 Dec 2010 00:00:00 +0100 4378272 http://www.medworm.com/index.php?rid=4378271&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21185830%26dopt%3DAbstract We present a molecular analysis of tumor overgrowing right proximal tibia with bone destruction metastatic to two groin lymph nodes. Fluorescent in situ hybridization analysis performed on paraffin-embedded tissue sections of primary tumor sample indicated one rearranged locus of EWSR1 gene and one additional red signal. Reverse transcription-polymerase chain reaction analysis revealed the presence of four different EWSR1/ATF1 chimerical transcripts in the tumor sample as well as in both metastatic lymph nodes. Two previously described transcripts EWSR1exon7/ATF1exon5 and EWSR1exon8/ATF1exon4, and two novel transcripts EWSR1exon7/ATF1exon4 and EWSR1exon9/ATF1exon4 were identified. Both novel transcripts were out-of-frame fusions and, therefore, most likely had limited biological impact in ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4378271 Fri, 24 Dec 2010 00:00:00 +0100 4378271 http://www.medworm.com/index.php?rid=4289508&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168405%26dopt%3DAbstract Authors: Ward BP, Tsongalis GJ, Kaur P Chronic lymphocytic leukemia (CLL) is the most common leukemia in the western world. Recently research, conducted primarily in basic science laboratories, has indicated a role for microRNAs (miRNAs) in the pathogenesis and prognosis of this disease. MiRNAs are small, non-coding, functional RNAs, that mediate post-transcriptional inhibition of messenger RNAs. Because miRNA expression levels in CLL patients differs from that of normal patients, there may be a role for these novel small molecules as biomarkers in this disease. PMID: 21168405 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4289508 Thu, 16 Dec 2010 00:00:00 +0100 4289508 http://www.medworm.com/index.php?rid=4289498&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168406%26dopt%3DAbstract We report the isolation and characterization of a population of tumor-initiating cells derived from an atypical meningioma. We identify a tumor-initiating population from an atypical meningioma, termed meningioma-initiating cells (MICs). These MICs self-renew, differentiate, and can recapitulate the histological characteristics of the parental tumor when transplanted at 1,000 cells into the flank regions of athymic nude mice. Immunohistochemistry reveals stem-like protein expression patterns similar to neural stem and progenitor cells (NSPCs) while genomic profiling verified the isolation of cancer cells (with defined meningioma chromosomal aberrations) from the bulk tumor. Microarray and pathway analysis identifies biochemical processes and gene networks related to aberrant cell cycle pro... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4289498 Thu, 16 Dec 2010 00:00:00 +0100 4289498 http://www.medworm.com/index.php?rid=4289527&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21167829%26dopt%3DAbstract CONCLUSIONS: Our study indicates that triptolide exert inhibitory effect on VSMC proliferation, inactivation of MAPK pathway and modulation of cell cycle proteins p21(cip1) and Rb are relating mechanisms. Triptolide drug-eluting stents attenuated neointimal formation after stent implantation in rabbit vessel. We believe that triptolid may potentially be useful in treating cardiovascular restenosis after PCI. PMID: 21167829 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4289527 Wed, 15 Dec 2010 00:00:00 +0100 4289527 http://www.medworm.com/index.php?rid=4236658&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21122804%26dopt%3DAbstract In conclusion, TRPS1 binds to the P2 promoter of the Runx1 gene and down-regulates Runx1 expression, which is necessary for normal cartilage formation. PMID: 21122804 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236658 Sun, 28 Nov 2010 00:00:00 +0100 4236658 http://www.medworm.com/index.php?rid=4236653&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21122805%26dopt%3DAbstract Authors: Shao HY, Miao ZY, Hui-Chen , Qin FX, Chen XC, Tan S, Zhang HJ, Wang L, Gao YJ, Yang ZL, Zhang L Nucleophosmin (NPM1) plays key roles in ribosome biogenesis, centrosome duplication, and maintenance of genomic integrity. NPM1 mutations have been recently identified as the most frequent genetic alteration in acute myeloid leukemia and are related with leukemogenesis. NPM1 mutations are involved in regulation of cell proliferation, cell cycle and apoptosis, however, the oncogenic potential of the NPM1 mutations has not been fully understood. Here, we investigated the role of NPM1 mutations in cell migration and invasion in vitro and explored its mechanisms. In our study, NIH3T3 cells were transfected with NPM1 mutation A (NPM1 mA), and the cell chemotactic response in vitro was ev... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236653 Sun, 28 Nov 2010 00:00:00 +0100 4236653 http://www.medworm.com/index.php?rid=4236682&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112324%26dopt%3DAbstract Authors: Ota Y, Chen J, Shin M, Nishiura H, Tokita K, Shinohara M, Yamamoto T Western blot analyses and monocyte chemoattraction analyses of guinea pig plasma and serum indicated the presence of a plasma protein indistinguishable from ribosomal protein S19 and the cross-linked dimerization of it gaining monocyte chemotactic capacity in association with blood coagulation as in the case of human. When coagula preformed in vitro were intraperitoneally inserted into guinea pigs, they were rapidly covered by macrophages within 24h concomitant with an intra-coagulum macrophage infiltration. Differences were observed between the surface macrophages and the penetrating macrophages in ultrastructural, histochemical and immunohistochemical analyses. The inserted coagula were resorbed by day 7. W... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236682 Fri, 26 Nov 2010 00:00:00 +0100 4236682 http://www.medworm.com/index.php?rid=4236679&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21115003%26dopt%3DAbstract Authors: Jha P, Jha P, Pathak P, Chosdol K, Suri V, Sharma MC, Kumar G, Singh M, Mahapatra AK, Chitra S Several single nucleotide polymorphisms of the TP53 gene have been reported, amongst which polymorphism in codon 72 (rs1042522) has received significant attention and shown to be associated with disease susceptibility in different cancer types. However, there are variable reports on this polymorphism in gliomas from worldwide with inconsistent results. In addition, the implications of other polymorphic loci are not much explored in gliomas. Hence, in the present study the TP53 sequence was analyzed for all polymorphism and mutations in a total of 84 gliomas of different types and grades from patients of Indian origin. The complete sequence of all coding exons (2 to 11) and introns 2,... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236679 Fri, 26 Nov 2010 00:00:00 +0100 4236679 http://www.medworm.com/index.php?rid=4236697&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21111728%26dopt%3DAbstract Authors: Berardi GR, Rebelatto CK, Tavares HF, Ingberman M, Shigunov P, Barchiki F, Aguiar AM, Miyague NI, Francisco JC, Correa A, Senegaglia AC, Suss PH, Moutinho JA, Sotomaior VS, Nakao LS, Brofman PS Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-D,L-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that recei... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236697 Thu, 25 Nov 2010 00:00:00 +0100 4236697 http://www.medworm.com/index.php?rid=4236733&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21110955%26dopt%3DAbstract Authors: Rivera-Toledo E, Huerta L, Larralde C, Lamoyi E Cells infected with the human immunodeficiency virus (HIV) can fuse with CD4(+) cells leading to the formation of multinucleated cells. The presence of multinucleated cells infected with HIV in the tissues of patients has been documented, although their cellular composition and role in AIDS pathogenesis is still under study. Here, we present evidence of in vitro heterotypic lymphocyte-monocyte fusion in cocultures of lymphocytic Jurkat T cells expressing the HIV-1 gp120/gp41 glycoproteins (Env) and CD4(+) monocytic THP-1 cells. Using a method previously characterized that involves the differential labeling of fusion partners with fluorescent probes and flow cytometry analysis after coculture, up to 20% of double fluorescent cells... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236733 Wed, 24 Nov 2010 00:00:00 +0100 4236733 http://www.medworm.com/index.php?rid=4236722&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21110956%26dopt%3DAbstract Authors: Skobowiat C, Calka J, Majewski M Sympathetic neurons are capable of extensive regeneration following axonal injury. To investigate the response to axotomy of colon-projecting neurons (CPN) localized in the porcine sympathetic chain ganglia (SChG), the retrograde Fast Blue (FB) tracer, axonal transection and double immunohistochemistry methods were applied. The CPN were localized exclusively in the lumbar SChG and displayed a predominantly catecholaminergic [i.e. Tyrosine Hydroxylase (TH)/Dopamine β Hydroxylase (DβH)] and Neuropeptide Y (NPY) positive phenotype under physiological conditions. Axotomy led to a significant decrease in TH/DβH production and a simultaneous increase in the neuropeptides Galanin (GAL) and Somatostatin (SOM), but not NPY or Vasoactive Intestinal Pe... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236722 Wed, 24 Nov 2010 00:00:00 +0100 4236722 http://www.medworm.com/index.php?rid=4236694&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21111729%26dopt%3DAbstract CONCLUSIONS: Upregulation of eNOS was seen both in infants with CDH and PPHN but was more marked in infants with CDH. These findings may have implications for understanding disease pathophysiology in cases with fatal outcome and development of novel therapies for neonatal pulmonary hypertension. PMID: 21111729 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236694 Wed, 24 Nov 2010 00:00:00 +0100 4236694 http://www.medworm.com/index.php?rid=4236683&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21111730%26dopt%3DAbstract Authors: Vandhana S, Deepa PR, Jayanthi U, Biswas J, Krishnakumar S Retinoblastoma (RB), the most common intra-ocular malignancy in children under 5years of age, has an estimated incidence of about 2000 a year in India, where most cases are in advanced stage at the time of diagnosis. Newer therapeutic approaches would reduce the morbidity of chemotherapy in children with RB. Fatty Acid Synthase (FASN), a lipogenic multi-enzyme complex, is minimally expressed in normal human tissues and over expressed in many cancers, making it an attractive target for cancer therapy. We analyzed RB tissues for FASN protein expression by immunohistochemistry, western blot, and ELISA, and FASN mRNA expression by RT-PCR. FASN expression was correlated with the clinico-pathological characteristics of the t... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4236683 Wed, 24 Nov 2010 00:00:00 +0100 4236683 http://www.medworm.com/index.php?rid=4176829&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21075101%26dopt%3DAbstract In this study, we examined the effect of TNF-α on the cell cycle inhibitor p27(kip) and apoptosis of SMCs in human carotid plaques, and the underlying mechanism. Both Forkhead box subclass o1 (FoxO1) and p27(kip) were more strongly expressed in symptomatic than asymptomatic atherosclerotic plaques. TNF-α significantly induced the expression of FoxO1 in asymptomatic plaque SMCs in a dose- and time-dependent manner via JNK signaling pathway. TNF-α also induced phosphorylation of FoxO1, resulting in its cytoplasmic translocation/nuclear exclusion of transcription factors. The effect of TNF-α was blocked by the PI3K inhibitor, LY294002. Meanwhile, TNF-α not only induced the p27(kip) expression and cell cycle arrest in G(0)-G(1) phase, but also enhanced caspase-3 activity and induced apopt... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4176829 Thu, 11 Nov 2010 00:00:00 +0100 4176829 http://www.medworm.com/index.php?rid=4176830&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21073868%26dopt%3DAbstract CONCLUSIONS: AI and p16 (ink4a) gene aberrant methylation may provide a potential universal biomarker for small-sized adenocarcinoma. PMID: 21073868 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4176830 Tue, 09 Nov 2010 00:00:00 +0100 4176830 http://www.medworm.com/index.php?rid=4130766&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21040720%26dopt%3DAbstract Authors: Subramaniam V, Ace O, Prud'homme GJ, Jothy S In the treatment of breast cancer, although a wide of choice of drugs and treatment modalities are available, drug resistance or drug toxicity pose a considerable challenge. Tranilast is a well tolerated drug used in the treatment of allergic disorders. Previous works in various models have shown that tranilast has the potential to be used as an anti-cancer drug. Hence, in this study using human breast cancer cell lines BT-474 and MDA-MB-231, we studied the effect of tranilast on cell growth, migration and ability to prevent colony formation in vitro, properties that are relevant to a possible therapeutic effect in breast cancer. We found that tranilast inhibits the growth of both breast cancer cell lines. In the cell migration expe... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4130766 Fri, 29 Oct 2010 00:00:00 +0100 4130766 http://www.medworm.com/index.php?rid=4130767&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036165%26dopt%3DAbstract In conclusion, proteasome inhibitor treatment used at a non toxic low dose has potential protective effects against oxidative stress due to chronic ethanol feeding. PMID: 21036165 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4130767 Thu, 28 Oct 2010 00:00:00 +0100 4130767 http://www.medworm.com/index.php?rid=4130769&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21035442%26dopt%3DAbstract Authors: Bauer S, Wanninger J, Neumeier M, Wurm S, Weigert J, Kopp A, Bala M, Schäffler A, Aslanidis C, Buechler C Type 2 diabetes is characterized by increased oxidative stress contributing to the development of cardiovascular disease (CVD). Monocytes are critically important in the pathogenesis of CVD and antioxidant enzymes like superoxide dismutase (SOD2) protect these cells from excessive reactive oxygen species (ROS). Adiponectin is an adipocyte-derived protein with atheroprotective function and the effect of adiponectin on monocyte SOD2 was analysed herein. Adiponectin upregulated SOD2 mRNA and dose- and time-dependently induced SOD2 protein in primary human monocytes. Elevated systemic free fatty acids (FFA) are commonly found in T2D patients and palmitic acid as well as oleic... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4130769 Wed, 27 Oct 2010 00:00:00 +0100 4130769 http://www.medworm.com/index.php?rid=4130768&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21035443%26dopt%3DAbstract Authors: Kawakatsu M, Kanno S, Gui T, Gai Z, Itoh S, Tanishima H, Oikawa K, Muragaki Y Smad3 is an intracellular signaling molecule in the transforming growth factor β (TGF-β) pathway that serves as a regulator of chondrogenesis and osteogenesis. To investigate the role of the TGF-β/Smad3 signaling in the process of fracture healing, an open fracture was introduced in mouse tibiae, and the histology of the healing process was compared between wild-type (WT) and Smad3-null (KO) mice. In KO mice, the bone union formed more rapidly with less formation of cartilage in the callus and eventually the fracture was repaired more rapidly than in WT mice. Alkaline phosphatase staining showed that osteoblastic differentiation in the fracture callus was promoted in KO mice. Additionally, TRAP st... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4130768 Wed, 27 Oct 2010 00:00:00 +0100 4130768 http://www.medworm.com/index.php?rid=4120002&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20970419%26dopt%3DAbstract Authors: Shen M, Chen W, Yu K, Chen Z, Zhou W, Lin X, Weng Z, Li C, Wu X, Tao Z Prostate cancer gene 3 (PCA3) encodes a prostate-specific mRNA that has shown promise as a prostate cancer（PCa）diagnostic tool and is detectable in prostate cancer cells shedding into urine after digital rectal examination (DRE). In our earlier studies, a tissue test for PCA3 appeared to have greater specificity for PCa. There, we performed a clinical evaluation of PCA3 mRNA assay in urine sediments after DRE enriched with prostate cells in a Chinese population. PCA3 mRNA was detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) in the urine sediments. PSA mRNA detected also by real-time FQ-RT-PCR was used to confirm the yield of prostate cells in the... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4120002 Tue, 19 Oct 2010 23:00:00 +0100 4120002 http://www.medworm.com/index.php?rid=4120005&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20969860%26dopt%3DAbstract We report an unusual case of PBL in a 69-year-old, HIV-negative non-immunocompromised man presenting with generalized lymphadenopathy. To our knowledge, this is the first case of PBL presented as primarily generalized lymphadenopathy in HIV-negative patients. Histologic examinations of cervical, inguinal and axillary lymph nodes demonstrated a neoplastic proliferation of large cells with extensive necrosis. The neoplastic cells formed sheets with relatively cohesive growth pattern interspersed by small lymphocytes and plasma cells. The large tumor cells expressed MUM1, OCT-2 and BOB.1, and were negative for CD138, CD38, AE1/AE3, melan A, PLAP, S100, vimentin, CD117, CD30, ALK-1, leukocyte common antigen (CD45), T-cell, B-cell and histolytic markers, CD56, CD10 and BCL-6. The proliferation ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4120005 Mon, 18 Oct 2010 23:00:00 +0100 4120005 http://www.medworm.com/index.php?rid=4120004&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20969861%26dopt%3DAbstract In this study, we established a cell line of malignant mesothelioma from a clinical specimen, and assessed the relationship between the expression of MT1-MMP and the invasion ability of that line as well as cultured cells of several other lines using the simple method that we created previously. We established a cell line from a clinical specimen from a patient with malignant mesothelioma. We assessed the invasive activities of MPM cells in an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that enabled us to visualize cell movements during invasion. To assess the role of MT1-MMP in the invasive activity of MPM cells, we knocked-down its expression by RNA interference (RNAi). The invasion assay with DL-CGH revealed that a high expression of MT1-MMP in MPM cells was a... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4120004 Mon, 18 Oct 2010 23:00:00 +0100 4120004 http://www.medworm.com/index.php?rid=4120003&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20969862%26dopt%3DAbstract In conclusion, our results suggest that there is a subtype of HCC with high expression of CD133, which might have more invasive characteristics by up-regulation of invasion-associated genes and EMT-associated genes. PMID: 20969862 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4120003 Mon, 18 Oct 2010 23:00:00 +0100 4120003 http://www.medworm.com/index.php?rid=4100868&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20965164%26dopt%3DAbstract Authors: Periasamy S, Chen SY, Liu MY PMID: 20965164 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4100868 Sun, 17 Oct 2010 23:00:00 +0100 4100868 http://www.medworm.com/index.php?rid=4100867&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20965165%26dopt%3DAbstract In conclusion, a combination of GSNO with L-cysteine led to significant increase in chloride efflux in CFBE41o(-) cells but the effect was transient and not sustained beyond minutes. PMID: 20965165 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4100867 Sun, 17 Oct 2010 23:00:00 +0100 4100867 http://www.medworm.com/index.php?rid=4100866&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20965166%26dopt%3DAbstract Authors: Liu W Cardiac fibrosis is defined as a progressive accumulation of fibrillar extracellular matrix (ECM) in the myocardium. The regulation of extracellular matrix remodeling is primarily mediated by cardiac fibroblasts (CF). Evidences suggest that various T lymphocyte phenotype differentially affects organ fibrosis through modulating CF collagen and MMP/TIMP gene expression, MMP activity and cardiac collagen cross-linking, leading to altered ECM composition. In regard to the importance of cytokines in cardiac fibrosis and heart failure, in this review, we will address the role of different T cell subsets in inflammation mediated cardiac fibrosis, from a distinct perspective of T cells and fibroblasts interaction. We conclude that in addition to preventive strategies, therapies ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4100866 Sun, 17 Oct 2010 23:00:00 +0100 4100866 http://www.medworm.com/index.php?rid=4086714&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20950609%26dopt%3DAbstract CONCLUSIONS: Over-expression of wild type c-RET is a characteristic molecular event of PTCs in Kuwait. The prevalence of RET/PTC is zero and among the lowest recorded in the world. PMID: 20950609 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4086714 Mon, 11 Oct 2010 23:00:00 +0100 4086714 http://www.medworm.com/index.php?rid=4086713&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20950610%26dopt%3DAbstract In this report, we evaluated the feasibility of cancer immunotherapy using Cep55/c10orf3 peptide for colorectal carcinoma (CRC). To evaluate the expression of Cep55/c10orf3 in CRC tissues, we performed immunohistochemical staining of using anti-Cep55/c10orf3 monoclonal antybody. Sixty-three percent cases showed weak positive for Cep55/c10orf3 in total 70 CRC cases. The Cep55/c10orf3 expression intention was was collated with high histological grade of CRC. Thus, we hypothesized that Cep55/c10orf3 can also be the target of CTLs in CRC cases. We generated CTLs from PBMCs of human leukocyte antigen (HLA)-A24-positive colorectal carcinoma patients using HLA-A24-restricted Cep55/c10orf3 peptides. Two of 6 colorectal cancer patients were reactive for the Cep55/c10orf3_193(10) peptide, which was ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4086713 Mon, 11 Oct 2010 23:00:00 +0100 4086713 http://www.medworm.com/index.php?rid=4033651&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20888811%26dopt%3DAbstract Authors: Qing X, French BA, Oliva J, French SW Over expression of FAT10 is characteristic of numerous types of carinoma including liver, gastric and colon carcinomas. In the case of colon carcinoma it is possible to determine at the point in the progression from the benign to the malignant process of colon cancer development by determining which stage in the neoplastic process FAT10 overexpression occurs. This stage was determined by measuring the intensity of fluoresence of immunohistochemically stained normal mucosa, tubular adenomas, hyperplastic polyps, serrated adenomas, villotubular, villous adenomas and invasive adenocarcinoma stages. Using this approach it was found the overexpression of FAT10 began at the serrated adenoma stage and continued to include the villous and villotub... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4033651 Wed, 29 Sep 2010 23:00:00 +0100 4033651 http://www.medworm.com/index.php?rid=4022134&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20868679%26dopt%3DAbstract Authors: Zejskova L, Jancuskova T, Kotlabova K, Doucha J, Hromadnikova I We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF1A) and total (GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n = 51) or female (n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals (n = 29). A strong positive correlation was observed between fetal-... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=4022134 Mon, 20 Sep 2010 23:00:00 +0100 4022134 http://www.medworm.com/index.php?rid=3994777&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20858485%26dopt%3DAbstract CONCLUSIONS: EBV is closely associated with NPC in Chinese patients. QD-FISH is a novel effective method for EBER detection, and has a moderate concordance with conventional ISH. PMID: 20858485 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology) Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=3994777 Fri, 17 Sep 2010 23:00:00 +0100 3994777 http://www.medworm.com/index.php?rid=3994776&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20858486%26dopt%3DAbstract Authors: Araújo AP, Frezza TF, Allegretti SM, Giorgio S Schistosomiasis mansoni is a chronic parasitic disease where much of the symptomatology is attributed to granuloma formation, an immunopathological reaction against Schistosoma eggs. To more clearly understand the immunopathology of schistosomiasis, the tissue microenvironment generated by S. mansoni infected mice was investigated. Using the hypoxia marker pimonidazole, we provide immunohistochemical evidence that hypoxia occurred in inflammatory cells infiltrated around the eggs and cells surrounding granulomas in the liver, intestine, spleen and lungs of infected mice. Hypoxia-inducible factor-1α (HIF-1α) was mainly expressed in inflammatory cells surrounding the eggs and in hepatocytes surrounding cellular and fibrocel... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=3994776 Fri, 17 Sep 2010 23:00:00 +0100 3994776 http://www.medworm.com/index.php?rid=3984050&cid=s_35559_32_f&fid=35559&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20840848%26dopt%3DAbstract Authors: Jahn JE, Best DH, Coleman WB The molecular pathogenesis of hepatocellular carcinoma is well-studied but not completely understood. We utilized a microcell-hybrid model of tumor suppression in rat liver tumor cells to facilitate the identification of liver tumor suppressor genes located on human chromosome 11. These investigations confirmed a liver tumor suppressor locus at human 11p11.2, identified Wt1 as a potential effector of 11p11.2-mediated tumor suppression, and subsequently identified human SYT13 as a strong candidate for the 11p11.2 liver tumor suppressor gene. In the studies presented here, we introduced SYT13 into the GN6TF rat liver tumor cell line to characterize a functional role for SYT13 in this model system. Transfected clones expressing an SDS-resistant dimer ... Experimental and Molecular Pathology journals http://www.medworm.com/rss/comments.php?id=3984050 Fri, 10 Sep 2010 23:00:00 +0100 3984050