MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Frontiers of Medicine in China' source. Sun, 21 Mar 2010 15:21:02 +0100 http://www.medworm.com/index.php?rid=3274098&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd1071675111x5qwp%2F Abstract  The surgical emergency treatments and curative effects of postrenal acute renal failure following obstruction due to upper urinary calculi in infants were evaluated. Of the 13 infants with postrenal acute renal failure following obstruction due to upper urinary calculi, 11 received retrograded catheterizations of the ureter with semi-rigid ureteroscopy (F 6.8), and two received open ureterolithotomy. The results showed that only one infant had anuresis and continuous reduction of hemoglobin 5 h after the open ureterolithotomy and received exploration via excision and peritoneal dialysis, and the remaining 12 patients well recovered in this group. The renal function of all the patients was restored without postoperative complications. It is concluded that the retro... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274098 Sun, 14 Feb 2010 06:42:04 +0100 3274098 http://www.medworm.com/index.php?rid=3274097&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw08j903t6870kw43%2F Content Type Journal ArticleCategory EditorialDOI 10.1007/s11684-010-0022-9Authors Sai-Juan Chen, Rui-Jin Hospital Affiliated with Shanghai Jiao Tong University School of Medicine Shanghai Insitute of Hematology Shanghai ChinaXiao-Ping Chen, Huazhong University of Science and Technology Tongji Hospital, Tongji Medical College Wuhan ChinaGuang-Biao Zhou, Chinese Academy of Sciences Institute of Zoology Beijing China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 Journal Volume Volume 4 Journal Issue Volume 4, Number 1 / March, 2010 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274097 Sun, 14 Feb 2010 06:42:04 +0100 3274097 http://www.medworm.com/index.php?rid=3274099&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv311223742233774%2F Abstract  China has been undertaking a profound reform on health care. Although more than 1.16 billion people have been covered by rural and urban medical insurance to date, the level of reimbursement from insurance is very limited, especially for critical diseases such as leukemia. This places heavy economic burdens on patients. Under these circumstances, systems innovation is imperative for the efficient utilization of limited funding. In this respect, certain valuable experience from other countries may prove helpful. The prospective payment system of Diagnosis-related Groups (DRGs), Clinical Paths, and the Comparative Effectiveness Analysis adopted by the National Institute of Health and Clinical Excellence (NICE, UK), can be fine tools to reduce medical costs and impro... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274099 Sun, 14 Feb 2010 06:42:03 +0100 3274099 http://www.medworm.com/index.php?rid=3274101&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu836414429632241%2F This study was conducted to evaluate the global and regional left ventricular twist by 2D strain in patients with anterior wall myocardial infarction (AMI). A total of 55 AMI patients were divided into two groups according to their ejection fraction (EF) values (group A: LVEF ⩾ 50%; group B: LVEF < 50%), and 35 normal people served as the control group. Using 2-dimensional strain software, global and regional LV rotation and displacement were obtained at two planes. Compared with the control group, patients of group A showed reduced peak LV twist of the anterior and anterior-septal wall (9.26±1.89 vs 10.74±2.67; 9.71±1.71 vs 11.36±2.29, both P < 0.05), but the radial displacement and global twist were maintained (P > 0.05). Differently, regional and global LV twist and ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274101 Sun, 14 Feb 2010 06:42:02 +0100 3274101 http://www.medworm.com/index.php?rid=3274100&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu60k55n1q8726m6x%2F Abstract  We conducted a trial to assess the influence of closure or nonclosure of the peritoneum and subcutaneous tissue on the clinical outcomes of cervical cancer patients who underwent radical hysterectomy with lower abdominal cross incision. This randomized controlled trial was performed on 158 cervical cancer patients in our hospital between January 2002 and June 2004. Eighty-two patients were allocated to the “closure” group and 76 patients to the “nonclosure” group. Results showed that non-closure of the peritoneum and subcutaneous tissue could shorten operation time and febrile duration, reduce antibiotics requirement, increase the volume of drainage and decrease the incidence of liquefaction of subcutaneous fat (P < 0.05). There was no difference in bloo... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274100 Sun, 14 Feb 2010 06:42:02 +0100 3274100 http://www.medworm.com/index.php?rid=3274102&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb213820n572v9g12%2F Abstract  One hundred and twenty-six patients above 80 years old with biliary diseases undergoing operations in Shaoxing People’s Hospital from Jan. 2002 to Jan. 2007 were analyzed retrospectively. All patients performed a preoperative stair-climbing test, and the risks for cardiopulmonary complications were evaluated with pairmatching and linear correlations analysis between stairclimbing height (h) and left ventricular ejective factor (EF), forced vital capacity (FVC) and forced expiratory volume in one second (FEV1). There was a significant difference in the incidence of cardiopulmonary complications among different stair-climbing heights. Stair-climbing heights were positively related with EF, FVC and FEV1. This suggests that the stair-climbing test is an effective and... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274102 Sun, 14 Feb 2010 06:41:59 +0100 3274102 http://www.medworm.com/index.php?rid=3274104&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa1t3t1030wq427j8%2F Abstract  Hepatitis B virus X protein (HBx), a 17-kd protein encoded by X gene of hepatitis B virus (HBV), has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of the study is to investigate the extracellular regulated protein kinases (ERKs) pathway of HBx on glomerular mesangial cell (GMC) proliferation and tumor necrosis factor-α (TNF-α) expression. The HBV X gene was amplified by polymerase chain reaction (PCR), inserted into the eukaryotic expression vector pCI-neo and confirmed by restriction endonuclease digestion and sequence analysis. PCI-neo containing HBV X gene (pCI-neo-X) was then transfected into cultured GMC line via liposome. GMC proliferation, TNF-α and its mRNA expression w... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274104 Sun, 14 Feb 2010 06:41:58 +0100 3274104 http://www.medworm.com/index.php?rid=3274103&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj353171h87268736%2F This study was to examine the functional significance of XPF promoter polymorphisms and their association with lung cancer risk. The function of XPF promoter polymorphisms was tested by a set of biochemical assays, and their effects on lung cancer risk were determined by a case-control analysis of 988 patients with lung cancer and 986 controls. The XPF — 673T allele showed a significantly higher transcriptional activity as compared with the −673C allele. The −673TT genotype was associated with a decreased risk of lung cancer compared with the CC genotype (adjusted OR = 0.62, 95% CI = 0.42–0.91; P = 0.015) and this effect was more significant among males (adjusted OR = 0.55, 95% CI = 0.35–0.86; P = 0.009), elder subjects (adjusted OR = 0.51, 95% CI = 0.30–0.86; P = 0.012), a... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274103 Sun, 14 Feb 2010 06:41:58 +0100 3274103 http://www.medworm.com/index.php?rid=3274105&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr348553t37p01692%2F This study was to investigate a better way to detect and differentiate primary, residual, recurrent nasopharyngeal carcinoma (NPC) lesions post-radiotherapy in patients with NPC by means of routine computed tomography (CT) in combination with 99Tcm-sestamibi single photon emission computed tomography (99Tcm-MIBI SPECT). Forty-eight patients with histologically confirmed primary NPC underwent 99Tcm-MIBI SPECT at the 3rd month before and after radiotherapy, and at the 6th month after radiotherapy. All patients had contemporaneous CT examinations. Histopathologic results and/or clinical follow-up data (over 18 months) were used as the golden standard for evaluating residual/recurrent lesions. The radioactive count ratio of nasopharynx to scalp was obtained as the MIBI uptake index (MUI). ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274105 Sun, 14 Feb 2010 06:41:57 +0100 3274105 http://www.medworm.com/index.php?rid=3274108&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F862116745k812219%2F This study investigated the molecular types of the Cryptococcus neoformans species complex isolates and their clinical manifestations among human immunodeficiency virus (HIV)-positive and HIV-negative patients in southeast China in the past 15 years. The molecular types of 109 isolates from 108 patients were analyzed by the PCR fingerprinting method, sequences of internal transcribed spacers of rDNA (ITS region), and sequences of the capsule-associated gene (CAP59). In HIV-positive patients, clinical isolates were grouped into molecular types VNI (75%, 15/20), VNII (15%, 3/20), and VNIII (10%, 2/20). In HIV-negative patients, the majority of the clinical isolates were grouped into molecular types VNI (72%, 64/89), VNII (13%, 12/89), VGI (12%, 11/89), VNIII (1%, 1/89), and VGII (1%, 1/... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274108 Sun, 14 Feb 2010 06:41:56 +0100 3274108 http://www.medworm.com/index.php?rid=3274107&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg33012j7782388p0%2F Abstract  Stroke is a major cause of adult death and disability worldwide. Epidemiological and animal studies have provided strong evidence that the pathogenesis of stroke is multi-factorial and induced by a combination of environmental and genetic risk factors, but the identification of individual causative variants remains little known. Genetic influences are likely to be polygenic with small effect sizes, and stroke itself consists of a number of different subtypes which may each have different genetic profiles. In addition, various ethnic populations may have different stroke risk, such as Asian race. The reasons for high risk of stroke among the Chinese, especially hemorrhagic stroke, remain unknown. Most human studies have taken a candidate gene approach using case-co... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274107 Sun, 14 Feb 2010 06:41:56 +0100 3274107 http://www.medworm.com/index.php?rid=3274106&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fqg037p3248521251%2F Abstract  Cardiovascular diseases (CVD) cause one third of all deaths in China, and the number is anticipated to double by 2020. They also greatly result in disability and adjusted life year loss. The prevalence of CVD has become a new burden for China, due to an aging population, smoking, and changes in dietary habits and lifestyles. As the largest developing country, China needs to tailor her own national policies for managing CVD with full consideration of epidemiology, local needs, and affordability. Smoking cessation, increased physical activity, control of hypertension and hypercholesterolemia, and maintenance of a “traditional Chinese” diet should be important strategies for reducing the burden of CVD in China. Health officials in China should take their responsi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274106 Sun, 14 Feb 2010 06:41:56 +0100 3274106 http://www.medworm.com/index.php?rid=3274109&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy4t1473p1622u822%2F Content Type Journal ArticleCategory News & ViewsDOI 10.1007/s11684-010-0024-7 Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 Journal Volume Volume 4 Journal Issue Volume 4, Number 1 / March, 2010 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274109 Sun, 14 Feb 2010 06:41:54 +0100 3274109 http://www.medworm.com/index.php?rid=3241573&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx728168542415260%2F Abstract  Mitogen-activated protein kinase (MAPK) signaling pathway, one of the most important signaling pathways in eukaryotic organism, is involved in multiple cellular events such as cell growth, differentiation, and apoptosis. MAPK is of great importance to the normal function of organisms, while its dysfunction results in various diseases. So far, inhibitors specifically against each subfamilies of MAP kinase have been developed, while more endeavors are needed to discover the compounds selectively targeting a particular subfamily member. Most of the kinase inhibitors exert their functions in an ATP-competitive way or a non-ATP-competitive way. Further studies on the effective mechanism of the MAPK inhibitors and their therapeutic roles in the treatment of diseases are... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241573 Wed, 03 Feb 2010 06:56:08 +0100 3241573 http://www.medworm.com/index.php?rid=3241574&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn43n332v1m8r7752%2F Abstract  Venous thromboembolism (VTE), including deep vein thrombosis and pulmonary embolism, carries significant mortality and morbidity. The most important and effective way to reduce VTE incidence is to identify the patients at risk and give necessary prevention. VTE is a multifactorial and complicated disorder. Major risk factors for VTE include surgery and trauma, acute medical illness, active cancer and pregnancy. Genetic factors increase susceptibility to the disease and are useful in predicting the development of VTE. Gene-gene and gene-environment interactions alter and magnify the clinical picture in this disorder. This brief review summarizes some selected clinical and genetic risk factors for VTE based on the current research in China. Content Type Journal Ar... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241574 Wed, 03 Feb 2010 06:56:07 +0100 3241574 http://www.medworm.com/index.php?rid=3241576&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd4w02m174712q633%2F This study aims to investigate the genetic association between the polymorphism of cytosolic phospholipase A2 (cPLA2) family genes and schizophrenia among Han Chinese in the northern part of China. The polymerase chain reaction-based ligase detection reaction (PCR-LDR) was applied to detect the genotype ten single nucleotide polymorphisms (SNPs) of cPLA2 family genes among 201 pedigrees consisting of fathers, mothers and affected offsprings with schizophrenia. The pedigrees were collected from 2000 to 2006. Haplotype relative risk (HRR) test, transmission disequilibrium test (TDT), haplotype transmission analysis and multiple locus analysis were conducted to analyze the genotyping data. The genotypic frequency of cPLA2 gene did not deviate from Hardy-Weinberg equilibrium either in ca... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241576 Wed, 03 Feb 2010 06:56:06 +0100 3241576 http://www.medworm.com/index.php?rid=3241575&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fwtv1u0417tr50817%2F Abstract  Normal brain function requires continuous supply of oxygen and glucose in a strict manner. The close spatial and temporal relationship between neural activity and cerebral blood flow (CBF) is termed as neurovascular coupling, which is dependent or not dependent on astrocytes. The increase in flow evoked by brain activity is mediated by the concerted action of multiple mediators that originate from different cells and act at different levels of the cerebral vasculature. More studies are needed for further understanding the precise mechanisms underlying neurovascular coupling, especially the implications of astrocytes. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-010-0019-4Authors You Zhou, Dalian Medical University Institute for Brain Disorders ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241575 Wed, 03 Feb 2010 06:56:06 +0100 3241575 http://www.medworm.com/index.php?rid=3241577&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv675075474m29385%2F This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all P ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241577 Wed, 03 Feb 2010 06:56:05 +0100 3241577 http://www.medworm.com/index.php?rid=3241578&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Flv75603282668807%2F This study aimed to identify functional single nucleotide polymorphisms in the cyclooxygenase-2 gene promoter and evaluate their effects on the risk of primary hepatocellular carcinoma (HCC) with hepatitis B virus (HBV) infection. We conducted a population-based, case-control study enrolling 630 Han Chinese people in Hubei province. Subjects included primary HCC patients with HBV infection (n = 210), chronic hepatitis B cases (n = 210) and healthy Han Chinese (n = 210). −1195G/A polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing analysis. We found -1195A allele carriers had a higher risk of HCC with HBVinfection (OR, 0.72; 95% CI, 0.548–0.946). The −1195A allele might be used as a marker in screening indiv... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241578 Wed, 03 Feb 2010 06:56:04 +0100 3241578 http://www.medworm.com/index.php?rid=3241579&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4k701w5514gn3837%2F Abstract  A community-based multi-center randomized controlled trial was conducted to evaluate the effectiveness of blood pressure control for hypertension patients in communities in urban Shanghai by integrated intervention. At present, patients (n = 1395) from four communities have completed follow-up for one year, including the intervention group (n = 921) and usual care group (n = 474). The intervention programs included disease management by a care manager. Blood pressure of each patient was measured regularly. Compared with the control group, the net change of mean systolic blood pressure (SBP) was − 6.75 (95% CI: − 7.79 to − 5.71, P < 0.001) mmHg, mean diastolic blood pressure (DBP) was − 4.29 (95% CI: − 5.08 to − 3.49, P < 0.001) mmHg, and mean pulse ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241579 Wed, 03 Feb 2010 06:56:03 +0100 3241579 http://www.medworm.com/index.php?rid=3241580&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb1283k8470p72283%2F Abstract  Severe sepsis and septic shock affect millions of patients and are major causes of mortality worldwide. Advancements in treatment and disease management led to a decline in in-hospital mortality from 27.8% (1979–1984) to 17.9% (1995 to 2000). In this article, we systemically review recent guidelines for the management of severe sepsis and septic shock published in 2008 by the International Surviving Sepsis Campaign Guidelines Committee. The 2008 Surviving Sepsis guidelines recommend protocolized resuscitation with goals to maintain central venous pressure ⩾ 8–12 mmHg, mean arterial pressure ⩾ 65 mmHg, urine output ⩾ 0.5 mL·kg−1·h−1 and central venous oxygen saturation ⩾ 70% (or mixed venous ⩾ 65%). Further fluid administration, transfusion of pac... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241580 Wed, 03 Feb 2010 06:56:02 +0100 3241580 http://www.medworm.com/index.php?rid=3224111&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F137463jv2r121u13%2F Abstract  Vulnerable atherosclerotic plaque rupture leading to thrombosis is the major cause of acute coronary syndrome (ACS). Studies on the pathophysiologic mechanism of both ACS and plaque stabilizing treatment are driving the development of animal models of vulnerable plaque. In our laboratory, we established animal models of plaque rupture and thrombosis in rabbits and mice that are similar to human plaque rupture. Potential mechanisms involved in plaque vulnerability were studied from the inflammation-immunity, proliferation-apoptosis, oxidative stress and biomechanics aspects. Imaging markers and biomarkers were used to detect vulnerable plaques, including high frequency duplex ultrasound, intravascular ultrasound (IVUS), intravascular ultrasound elastography, magnet... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3224111 Thu, 28 Jan 2010 18:05:05 +0100 3224111 http://www.medworm.com/index.php?rid=2986913&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff0p5540m73864335%2F Abstract  The authors performed a meta-analysis of case-control studies that addressed whether reproductive factors and oral contraceptive use were associated with breast cancer by searching the MEDLINE, PubMed, Proquest, Embase, ScienceDirect, African Healthline, BMJ Health Intelligence and Chinese Periodical net databases for all English-language and Chinese-language papers published from January 1, 1997 to December 31, 2007. A total of 15 studies calculating pool ORs indicated that menopausal age >50 yr [odds ratio (OR), 1.39; 95% confidence interval (CI), 1.22–1.57] and oral contraceptive use (OR, 2.12*, “*”: summary OR was adjusted; 95% CI, 1.24–3.62) were correlated with the increase in breast cancer risk while the summary OR based on number of full-term pr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2986913 Tue, 10 Nov 2009 18:25:04 +0100 2986913 http://www.medworm.com/index.php?rid=2986912&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fyt8414142245t5n4%2F Abstract  Few studies have been conducted to assess the level of physical activity (PA) of women in China. The purpose of this study was to describe the prevalence of PA among women in Wuhan and explore sociodemographic and behavioral correlation to PA for the population. A total of 1359 community-dwelling women (42.97±13.60 years old) participated in this study. The long-version IPAQ was used to define the level of PA. 45.7% of women were considered physically active, and 27.5% were vigorously active. The proportion of physical inactivity was around 26.8%. The total and 4 domains of PA showed significant age, education level, and occupation variation. PA tended to be lower as the age declined. PA in transportation, housework, leisure time, and the total tended to be the l... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2986912 Tue, 10 Nov 2009 18:25:04 +0100 2986912 http://www.medworm.com/index.php?rid=2979239&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F44u3768311415714%2F Abstract  A murine primary mammary tumor model was established to investigate the treatment with ginsenosides Rg3. The relationship between ginsenosides Rg3 and primary mammary tumor was explored. Mammary tumor was induced by using the 7,12-dimethybenz(a)anthracene (DMBA). Ginsenoside Rg3 was employed for treatment. The incidence of mammary tumor in every group was compared, and the expressions of vascular endothelial growth factor (VEGF) and microvessel density (MVD) were detected by immunohistochemical method. The cell cycle and apoptosis percentage were determined by means of flow cytometry. The incidence of tumor in treatment group was significantly lower than that in control group (60.00% vs 33.33%, P < 0.05). The average diameter of mammary tumor was (0.86±0.27) cm... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979239 Sat, 07 Nov 2009 19:05:56 +0100 2979239 http://www.medworm.com/index.php?rid=2979241&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb24401666211m522%2F This study is mainly aimed at evaluating the expression of PC-cell-derived growth factor (PCDGF) in breast cancer and breast adenofibroma, and to compare with other commonly used clinical pathological indices, then to investigate the diagnostic and targeted therapeutic purpose of PCDGF in breast cancer tissue. In this study, we detected the expression of PCDGF, p53 and CerbB-2 in breast cancer tissue and the expression of PCDGF in breast adenofibroma tissue by immunohistochemical method, and analyzed the relationship between them. We found that PCDGF was expressed in most breast cancer tissue, but was not in breast adenofibroma tissue, and the expression of PCDGF was related with the tumor’s pathological category and the expression of estrogen receptor (ER) and progesterone recepto... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979241 Sat, 07 Nov 2009 19:05:50 +0100 2979241 http://www.medworm.com/index.php?rid=2979240&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbn8678u237207226%2F In conclusion, the EBV LMP2 DNA vaccine can induce a strong humoral immune response in mice. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0087-5Authors Jianqing Pan, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaQin Zhang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaDaowen Wang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979240 Sat, 07 Nov 2009 19:05:50 +0100 2979240 http://www.medworm.com/index.php?rid=2971554&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy553174670p36381%2F Abstract  The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated. Rat models of myocardial hypertrophy were established by using abdominal aorta partial coarctation method. Forty-eight rats were randomly divided into sham group (S group), model group (M group), valsartan treatment group (X group), low-dose tanshinone treatment group (LD group), medium-dose tanshinone treatment group (MD group), and high-dose tanshinone treatment group (HD group) (n = 8 in each group). Left ventricular mass index (LVMI), left ventricular posterior wall (LVPW), and septal thickness (IVS) were detected by high frequency ultrasonography. Myocardial fiber diameter (... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971554 Fri, 06 Nov 2009 19:47:49 +0100 2971554 http://www.medworm.com/index.php?rid=2971555&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp75050n5357801gh%2F Abstract  Thl7 cells, a special kind of auxiliary type T cells, can secrete IL-17A, IL-17F, IL-21, IL-22, etc., as a newly discovered T-cell subset in recent years. As a different subset from the Thl and Th2 cells, Th17 cells play an important role in the development of a variety of autoimmune diseases. A current study shows that the IL-6 inflammatory response of the organization in combination with the occurrence of TGF-β can induce the differentiation of Thl7 cells. IL-23 can promote the production of IL17, as well as participate in amplification and maintenance of the survival of IL-l7 generating cells. In this process, STAT3 and ROR-γt are key transcription factors for the growing of Thl7 cells. As our knowledge of Th17 family members is rapidly growing and changing, ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971555 Fri, 06 Nov 2009 19:47:45 +0100 2971555 http://www.medworm.com/index.php?rid=2971556&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjru424647q474138%2F Abstract  The aim of this paper was to investigate the prevalence of human papilloma virus (HPV) infection in women under 30 years old with cervical intraepithelial neoplasia (CIN) or cervical cancer, in order to provide a basis for cervical cancer prevention and treatment. We recruited 2052 female cases from the education system in Futian District, Shenzhen city, from April 2006 to April 2008, with age ranging from 22 to 60 years old. Secondgeneration hybrid capture test was done for detection of female genital tract HPV and cervical colposcopy for screening CIN or cervical cancer. The prevalence of HPV and cervical precancerous lesions or cervical cancer was evaluated in different age groups of women. The HPV positive rate was 247/2052 (12.00%), and 35/2052 (1.71%) of the... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971556 Fri, 06 Nov 2009 19:10:08 +0100 2971556 http://www.medworm.com/index.php?rid=2971557&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa6730022q0008205%2F Abstract  The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea. A total of 98 patients with stage IV cancers were recruited and divided into a prevention group (52 patients) and a control group (46 patients). All patients received the chemotherapy including irinotecan. The prevention group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days; the control group did not receive any prevention. The use of gentamycin and sodium bicarbonate resulted in significantly higher stool pH (P<0.001), while the incidence of diarrhea by irinotecan was reduced (prevention group 13.70% versus control group 34.83%; P<0.001). Gentamycin combined with sodium bi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971557 Fri, 06 Nov 2009 19:10:07 +0100 2971557 http://www.medworm.com/index.php?rid=2971558&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr478m83775p50v20%2F Abstract  The expression of signal transducer and activator of transcription 6 (STAT6) and nuclear factor-κB (NF-κB) in the colonic mucosa of patients with ulcerative colitis (UC) was examined. Real-time polymerase chain reaction and immunohistochemistry were used to detect the expression of STAT6 and NF-κB p65 at both mRNA and protein levels in the colonic mucosa of patients with UC and healthy volunteers. The results showed that the expression levels of STAT6 and NFκB p65 in the colonic mucosa of patients with UC were significantly higher than in normal controls at both mRNA and protein levels. These data suggest that STAT6 and NFκB p65 perhaps play an important role in the pathogenesis of UC and underscore the potential value of anti-UC strategies in the clinical ma... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971558 Fri, 06 Nov 2009 19:10:03 +0100 2971558 http://www.medworm.com/index.php?rid=2968962&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff38347wr1hkt266h%2F Abstract  This paper is aimed to examine if changes in platelet-derived growth factor (PDGF) expression at different stages of cervical cancer are related to the variation in blood vessel density (BVD) and lymphatic vessel density (LVD) to evaluate the relationship between PDGF expression and stages and metastasis of cervical cancer. Polymerase chain reaction (PCR) and RT-PCR were used to detect the expression levels of PDGF in 45 cervical cancer tissue samples (the experimental group). The samples were immunohistochemically stained with monoclonal antibodies D2-40 and CD34, and BVD and LVD were measured. The expressions of PDGF-A, -B, and -D were all higher in the experimental group than in the control group (P<0.05); no significant difference was found in the expression... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968962 Thu, 05 Nov 2009 07:45:23 +0100 2968962 http://www.medworm.com/index.php?rid=2968963&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn226q4261r476752%2F Abstract  The effects of compound Sophorae Flavescentis Jiechangrong capsule (CSFJC) on the expression of nuclear factor-κB p65 (NF-κB p65) and signal transducer and activator of transcription 6 (STAT6) in the intestinal mucosa of patients with ulcerative colitis and the possible mechanism were investigated. Eighteen patients with ulcerative colitis were randomly divided into a traditional Chinese medicine (TCM) group (n = 11) treated by CSFJC and a western medicine (WM) group (n = 7) treated by Sulfasalazine tablets. The treatment duration lasted eight weeks. Before and after the treatment, the symptoms and the physical signs were observed, and the routine stool test, the colonoscopy, and pathological examination were performed in the two groups. The expression levels of ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968963 Thu, 05 Nov 2009 07:45:21 +0100 2968963 http://www.medworm.com/index.php?rid=2968964&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw78v565532025263%2F We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s11684-009-0076-8Authors Lenan Shao, Huazhong University of Science and Technology Department of Stomatology, Tongji Hospital, Tongji Medical College Wuhan 4300... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968964 Thu, 05 Nov 2009 07:45:19 +0100 2968964 http://www.medworm.com/index.php?rid=2968965&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm6083632r37g3273%2F Abstract  This paper is aimed to investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma GBC-SD cells. The viability of human gallbladder carcinoma GBC-SD, GBC-SD/enhanced green fluorescent protein (EGFP), and GBC-SD/survivin cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and mRNA and protein of survivin were tested by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. After the cells were treated with cisplatin (DDP) (3.0 µg/mL) for the same time, cell survival rate and IC50 was detected with MTT, cell apoptosis was detected with fluorescence-activated cell sorting (FACS), and the nuclear alteration was observed by TdT-mediated deoxyuridine triphosphate nick... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968965 Thu, 05 Nov 2009 07:45:18 +0100 2968965 http://www.medworm.com/index.php?rid=2968966&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj107586317283j05%2F Abstract  This paper aims to evaluate the effects and the possible mechanisms of atorvastatin on tumor growth and metastasis in a xenograft tumor model. Twenty-four female athymic BALB/C mice with MDA-MB-435 xenograft tumors were randomly assigned to three groups: a control group, a low-dose atorvastatin treatment group, and a high-dose atorvastatin treatment group. The mice in the treatment groups began to be administered with atorvastatin (10 or 20 mg/kg per day) when the xenograft tumors reached 1 cm in diameter. At the end of the experiment, the tumor volume and weight and the lung metastasis colonies of each mouse were measured. Western blotting was applied to detect phosphorylation of protein kinase B (PKB, Akt), extracellular signal regulated kinase (ERK), c-Jun N-te... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968966 Wed, 04 Nov 2009 21:51:14 +0100 2968966 http://www.medworm.com/index.php?rid=2941341&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl20536765rr6p15n%2F Abstract  The aim of this study was to investigate the inhibitory effects of recombinant adenovirus vector carrying tissue inhibitor of metalloproteinase-3 (RAd-TIMP-3) against degeneration of rabbit intervertebral disc. Thirty Japanese white rabbits of 4 months old were randomly divided into 5 groups. Mild or moderate rabbit lumbar disc degeneration model was constructed with the controllable axial loading device by imposing 98 N pressure at the discs for 2 weeks. Various doses of virus were injected into the degenerated discs as follows: 20 μL of normal saline in group 1; 20 μL of RAd66 (an empty adenovirus vector, 1.0 × 1010 OPU/mL) in group 2; and 20, 10, and 5 μL of RAdTIMP-3 (1.0 × 1010 OPU/mL) in groups 3, 4, and 5, respectively. Two weeks after the injection, th... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2941341 Wed, 28 Oct 2009 07:03:24 +0100 2941341 http://www.medworm.com/index.php?rid=2936604&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj4r30m15w53g7827%2F Abstract  p38 regulated/activated protein kinase (PRAK) plays a key role in cell senescence and tumor suppression. The aim of this study was to investigate if PRAK had effect on cell proliferation. The growth of PRAK +/+ and PRAK −/− mouse embryonic fibroblast (MEF) cells was measured by methylthiazoletetrazolium (MTT) colorimetric assay, and the proportion of the cell number in different phases of the cell cycle was analyzed by flow cytometry. The growth curves showed that the growth rate was notably decreased, and cell double time was elongated in PRAK −/− cells; moreover, the number of PRAK −/− cells was decreased by 44.5% compared with that of PRAK +/+ cells cultured for 96 h, suggesting that G2/M transition is inhibited in PRAK −/− cells. Meanwhile, ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2936604 Tue, 27 Oct 2009 18:07:25 +0100 2936604 http://www.medworm.com/index.php?rid=2936605&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2318566036757rr8%2F In conclusion, exercise training ameliorates insulin resistance. The reduction of the level of serum TNF-α and the increased expression of TNF-α in adipose tissue by exercise training may be involved in this mechanism. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0071-0Authors Hui Sun, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaXiuling Deng, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaFangxi Xiao, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaLulu Chen, Huazhong University of Science and Tech... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2936605 Tue, 27 Oct 2009 18:07:24 +0100 2936605 http://www.medworm.com/index.php?rid=2924264&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc8146m5157655p41%2F Abstract  Natriuretic peptide precursor A (NPPA) is synthesized, stored, and released by atrial myocytes. Previous studies have shown that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis. Rs5065 NPPA gene polymorphism leads to the translation of NPPA with two additional arginines and has been suggested to be associated with salt-sensitive hypertension. The purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphism and the risk of coronary heart disease (CHD) in Chinese Han population. We genotyped the single nucleotide polymorphism (SNP) rs5065 NPPA in the human NPPA gene in 1861 sex- and age-matched subjects, comprising of 904 CHD cases and 957 controls of Chinese Han population.... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924264 Thu, 22 Oct 2009 18:41:12 +0100 2924264 http://www.medworm.com/index.php?rid=2924263&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl0k1118720m25800%2F Abstract  The spinal epidural space is an uncommon presenting site in primary non-Hodgkin’s lymphomas, especially for children. A boy suffered spinal cord compression syndrome caused by primary spinal epidural non-Hodgkin’s lymphoma. Thoracolumbar magnetic resonance imaging (MRI) demonstrated an intraspinal mass. An operation was performed with gross total tumor removal. Histological examination revealed a non-Hodgkin’s B-cell lymphoma. Bone marrow aspiration was negative for lymphoma involvement. No other therapies (chemotherapy and/or radiotherapy) were performed according to the parents’ opinion. The patient died approximately one year after the operation due to brain metastases. The clinical course and imaging features were discussed with a review of literatures... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924263 Thu, 22 Oct 2009 18:41:12 +0100 2924263 http://www.medworm.com/index.php?rid=2924266&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu62674636t63v576%2F Abstract  In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis. The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000. The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. The cell cycle was analyzed by using DNA content analysis by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain. CDK1 gene was successfully silenced by its siRNA, and the CDK1 protein expression level was... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924266 Thu, 22 Oct 2009 18:41:11 +0100 2924266 http://www.medworm.com/index.php?rid=2924265&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu62616847342g711%2F Abstract  Bone remodeling in orthodontic treatment has been one of the most concerned issues. The purpose of this study was to characterize the changes in bone remodeling on both labial and lingual aspects of the anterior teeth during treatment and retention. Data of 55 extraction cases (41 females and 14 males) were collected at pretreatment, post-treatment, and follow-up stages. Bone thickness on both labial and lingual aspects of the upper and lower incisors were measured at the level of the center of resistance (UC and LC) and that of 3 mm apical to the upper and lower incisors’ center of resistance (UAC and LAC). For LC, with the significant lingual movement of point LIR (center of resistance of lower incisor; P < 0.001), no statistically significant changes in alv... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924265 Thu, 22 Oct 2009 18:41:11 +0100 2924265 http://www.medworm.com/index.php?rid=2906567&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw68210xq8656022p%2F This article presents a case of renal AML occurring in a 14-year-old girl with a definite diagnosis of TSC. She had been diagnosed with TSC at the age of three, and a schedule for close observation was disobeyed. At this time, she underwent a series of examinations: physical examination, ultrasonography, angiography, computed tomography (CT), and magnetic resonance imaging (MRI) scans. The physical examination showed adenoma sebaceum in a butterfly paranasal distribution, and a mass was palpated in the left upper quadrant. There were no neurological deficits. Imaging studies (including ultrasonography, angiography, CT, and MRI) of the abdomen showed a large heterogeneous mass arising from the left kidney. Partial nephrectomy was performed. The pathological diagnosis was hemorrhagic r... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2906567 Sat, 17 Oct 2009 17:22:54 +0100 2906567 http://www.medworm.com/index.php?rid=2661648&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F567733j010586278%2F This study was to investigate the regulation of proliferation and apoptosis by LY294002, an inhibitor of PI3K in cervical cancer cells and the expression of FLICE-like inhibitory protein (c-FLIP) in vitro. Human cervical cancer HeLa cells were used in this experiment and cultured. The cultured cells were treated with LY294002 at different concentrations (10, 25, 50 and 100 μmol/L) for 6, 12, 24, and 48 h before harvesting for evaluation. Cell viability was measured by 3-(4,5)-dimethylthiazol(-2-y1)-3,5-di-phenyltetrazoliumbromide (MTT) assay. Apoptosis was analyzed by flow cytometry. The expression of c-FLIP was detected by Western blot. Cell viability was inhibited by LY294002 significantly (P<0.05). Flow cytometry analysis revealed that cell apoptosis was significantly increased... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2661648 Thu, 30 Jul 2009 10:20:23 +0100 2661648 http://www.medworm.com/index.php?rid=2661649&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjq7k86p13u6n0045%2F Abstract  The aim of this paper is to investigate the relationship between hepatitis B virus (HBV) DNA levels during the course and the progression to cirrhosis with chronic hepatitis B. A total of 239 chronic hepatitis B patients confirmed by liver biopsy between 2001 and 2007 were followed up for a median of 28 months. Compared with the patients without cirrhosis, the patients progressed to cirrhosis were older and with higher HBV-DNA levels at end point. However, there was no significant difference in cirrhosis progression between different HBV-DNA groups at baseline (P = 0.531). Kaplan-Meier analysis showed higher HBV-DNA level at endpoint had increasing risk of cirrhosis (P = 0.019). The results of Cox model indicated that HBV-DNA levels at endpoint, stage of fibrosis, n... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2661649 Wed, 29 Jul 2009 07:04:15 +0100 2661649 http://www.medworm.com/index.php?rid=2643075&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm67038251030v122%2F Abstract  The eukaryotic expression vector of human arresten gene was constructed and its secretive expression human embryonic kidney (HEK 293) cells was detected. Human arresten gene was amplified from recombinant plasmid pGEM-Arr by polymerase chain reaction (PCR), and then digested with restriction endonucleases BamH I and EcoR I. The target fragment was inserted into the BamH I and EcoR I restriction sites of eukaryotic expression vector pSecTag2 to construct pST-AT. Restriction analysis and DNA sequencing indicated that the arresten gene was successfully inserted into pSecTag2. The recombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent, and the expression of the target gene was detected. RTPCR revealed that the mRNA of the t... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643075 Sat, 25 Jul 2009 08:53:42 +0100 2643075 http://www.medworm.com/index.php?rid=2643076&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm846k66152vnv662%2F This study aimed to investigate the sensory innervation of the anterior eye segment in rats by retrograde tracing with 1,1-dioleyl-3,3,3,3-tetramethylindocarbocyanine, 4-chlorobenesulfonate (FAST Dil) injected into the anterior chamber. The sensory innervation of distinct elements of the anterior segment of the rat’s eye, i.e. the cornea, ciliary body, iris, and trabecular meshwork, were studied by retrograde tracing using FAST Dil as a tracer. FAST Dil was injected into the anterior chambers of the rat’s eyes. The animals were sacrificed at different time points, i.e., 2, 3, 4, and 5 days after the injection. FAST Dil localization in trigeminal ganglions was studied with a fluorescent microscope. Two days after FAST Dil injection into the anterior chambers, the cornea, the ciliar... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643076 Sat, 25 Jul 2009 04:36:09 +0100 2643076 http://www.medworm.com/index.php?rid=2643077&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F15703w6211872166%2F Abstract  In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0057-yAuthors Xunli Xia, Huazhong University of Science and Technology China-UK HUST-RRes Genetics... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643077 Sat, 25 Jul 2009 04:12:36 +0100 2643077 http://www.medworm.com/index.php?rid=2643079&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy4h01kn336p62833%2F Abstract  Abstract The purpose of this article is to determine the effect of a well-designed combined aerobic, resistance, and extension exercise program on bone mineral density (BMD) in postmenopausal women. The population comprised 45 postmenopausal women, who exercised over 12 months (exercise group), and 36 women who served as a non-training control group. BMD of the hip, and lumbar spine was measured at the baseline and 12th month. Repeated measurement analysis of variance and nonparametric test were utilized to compare differences between the exercise group and controls. Thirty-six out of 45 persons in the exercise group and 36 controls completed the study. Average compliance was 82.2% for the whole exercise group at the 12th month. All the subjects had decreased BMD,... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643079 Sat, 25 Jul 2009 04:12:34 +0100 2643079 http://www.medworm.com/index.php?rid=2643078&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8833rjlm1745x050%2F In this study, we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion. TC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis. Western blot and enzyme-linked immunosorbent assay (ELISA) analysis showed that the TC-tagged core protein, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) could be expressed in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag into core protein in some degree, but cells transfected with the HBV vector coul... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643078 Sat, 25 Jul 2009 04:12:34 +0100 2643078 http://www.medworm.com/index.php?rid=2643080&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx577786770g15607%2F Abstract  The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H0 (non-transfected Hela cells), H1 (pGenesil-1 empty plasmid-transfected Hela cells), and H2 (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O2) and normoxia for 48 h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch test and the invasion assay were applied to examine the invasion in each group. The expression of HIF-1α, E-cadherin, and β-catenin in tumor grafts was assayed immunohistochemically. Western blot results revealed that the bands of HIF-1α, E-cadhe... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643080 Sat, 25 Jul 2009 04:12:33 +0100 2643080 http://www.medworm.com/index.php?rid=2638958&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm883581477347072%2F This article reviews the current knowledge of viral hepatitis (types B and C) at the level of miRNA and tries to outline areas of potential studies. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-009-0055-0Authors Gang Li, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 ChinaXiaojia Xiong, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2638958 Wed, 22 Jul 2009 01:47:02 +0100 2638958 http://www.medworm.com/index.php?rid=2638957&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm7331283j041n372%2F Abstract  Lanthanum salt is a prescription drug, but its underlying functions and mechanisms are not fully understood currently. To explore the potential therapeutic value of lanthanum chloride, cytotoxicity test was applied to investigate its effects on cell proliferation. Furthermore, we observed its influence on pro-oncogene c-met by reverse transcription polymerase chain reaction (RT-PCR). In MCF-7 cell line, lanthanum chloride repressed cell proliferation at high concentrations but had no significant inhibition effect on cell growth at low concentrations. However, we observed that lanthanum chloride repressed c-met transcription at a low concentration. This may suggest that lanthanum chloride is a potent drug to inhibit the high expression of c-met in carcinoma cells an... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2638957 Wed, 22 Jul 2009 01:47:02 +0100 2638957 http://www.medworm.com/index.php?rid=2632315&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5qrtwu42786l36p5%2F Abstract  The feasibility and clinical therapeutic effects of laparoscopic sigmoid vaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser syndrome (MRKHs) were explored. The records of 11 MRKHs patients who underwent laparoscopic sigmoid vaginoplasty from 2003 to 2005 were reviewed, and long-term results were evaluated by follow-up. The mean operating time was 234 min (range, 130–300 min), the mean hospital stay was 9.4 days (range, 7–15 days), and the mean hemoglobin drop was 1.91 g/dL (range, 1.6–3.2 g/dL). A functional neovagina was created measuring 11 to 14 cm in length and two fingers in breadth in all patients. No introitus stenosis was observed. No intra-operative or postoperative bowel complication occurred. At the 3rd postoperative month, the first interc... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632315 Tue, 21 Jul 2009 15:17:42 +0100 2632315 http://www.medworm.com/index.php?rid=2632316&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv463507277h51348%2F Abstract  This paper aims to investigate the effects of Zhihuang (枳黄) decoction on CD14 expression in the lipopolysaccharide signal transduction pathway of alcohol-induced liver disease in rats. Seventy-five Wistar rats were randomly divided into three groups. Ethanol (56%, weight/volumn) was intragastrically administrated to 50 rats (14 mL/kg body weight per day) for 10 days to establish a model of alcohol-induced liver disease, and 25 of these 50 rats were treated with Zhihuang decoction simultaneously. Liver injury was evaluated by biochemical examination. The plasma content of endotoxin was assayed by biochemistry. The expression of CD14 mRNA and protein in rat liver was measured by reverse transcription polymerase chain reaction and immunohistochemistry, r... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632316 Tue, 21 Jul 2009 15:17:38 +0100 2632316 http://www.medworm.com/index.php?rid=2632317&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc68713m76226032q%2F Abstract  To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor (VEGF) and the possible mechanism of VEGF, the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group, VEGF-treated group and VEGF + N-nitro-L-arginine methyl ester (L-NAME)-treated group. The absorbance (A) value of vascular endothelial cells and the levels of prostaglandin (PGI2), endothelin-1 (ET-1) and von Willebrand factor (vWF) in the supernatant were observed by water-soluble tetrazolium salt assay, radioimmunoassay and enzyme-linked immunosorbent assay. The A values and PGI2 in VEGF-treated group and VEGF + L-NAME-treated group were higher than those in control group (P < 0.05 and P < 0.01). The ET-1 and vWF were sign... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632317 Tue, 21 Jul 2009 15:17:35 +0100 2632317 http://www.medworm.com/index.php?rid=2632318&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk7g0564453x1q054%2F Abstract  In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expression of the murine cdk4 gene in vitro. Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants were transfected by lipofectamine into BV-2 cells. The gene silencing efficacy rates of the 3 targets were compared by Western blotting. The cdk4-siRNA2 was the most effective i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632318 Tue, 21 Jul 2009 15:17:29 +0100 2632318 http://www.medworm.com/index.php?rid=2632319&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb288046r7273m054%2F Abstract  The study evaluated the value of ultrasound elastography in differentiating the benign and malignant superficial lymph nodes. A total of 112 subjects, including 82 patients with enlarged lymph nodes and 30 healthy volunteers, were recruited. All the subjects were examined by B-mode ultrasonography, power Doppler ultrasonography and elastography. Most of the patients were histopathologically confirmed by needle aspiration cytology and some patients were diagnosed by clinical data combined with follow-up findings. The sensitivity, specificity and accuracy of B-mode ultrasonography were 59.8%, 76.5% and 67.1%, those of the blood flow classification by power Doppler ultrasonography 77.0%, 82.3% and 79.4% and those of elastographic classification 74.7%, 97.1% and 84.5%... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632319 Tue, 21 Jul 2009 15:17:25 +0100 2632319 http://www.medworm.com/index.php?rid=2632320&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft5158642687231k2%2F Abstract  More and more molecular drugs based on targeted therapy have been utilized in the treatment of gynecologic cancer, especially in ovarian cancer. In this article, we systematically review the current targeted therapeutic trials running in clinic. Large, randomized trials have been conducted in the treatment of ovarian cancer, endometrial cancer and cervical cancer by usingsmall molecule, antisense, mutational gene as well as antibodies. Other planned or ongoing trials currently targeted at molecular markers which may play important roles in gynecological carcinogenesis and progression suggest that combination chemotherapy with molecular targeted therapy will ultimately be an important option. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-009-0052-... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632320 Tue, 21 Jul 2009 15:17:24 +0100 2632320 http://www.medworm.com/index.php?rid=2612527&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F770m278451576442%2F Abstract  Lung cancer is one of the most common human cancers and the number one cancer killer in the United States. In general, lung cancer includes small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), but NSCLC accounts for approximately 90% of lung cancer. The early diagnosis and therapy of lung cancer still presents a big challenge because validated screening tools, which can improve current early detection to reduce mortality from lung cancer, do not exist. Over the last decade, molecular genetic abnormalities have been described in NSCLC, including chromosomal aberrations, overexpression of oncogenes, and deletion and/or mutations in tumor suppressor genes. These molecular markers in NSCLC demonstrated close associations with the development of lung c... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2612527 Thu, 16 Jul 2009 00:16:31 +0100 2612527 http://www.medworm.com/index.php?rid=2604048&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbt76321492496555%2F Abstract  Proteinase-activated receptors (PARs) are a novel subclass of seven transmembrane-spanning, G protein-coupled receptors. PAR-1 and PAR-2 are widely expressed in a variety of cells and are found to be involved in many physiological and pathological processes including inflammation and immune response. However, little is known about the function of PAR-1, 2 in acute graft vs host disease (GVHD). In the present study, we first detected the expression of PAR-1, 2 protein and mRNA in a murine model of acute GVHD using the methods of immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction (PCR). Syngeneic hematopoietic stem cell transplantation (HSCT) mice served as controls. The relative gene expression level of PAR-1 was significantly i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2604048 Tue, 14 Jul 2009 10:55:05 +0100 2604048 http://www.medworm.com/index.php?rid=2604049&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3586143n46162612%2F This study aimed to investigate the protective effect of nicotine on dopaminergic neurons and its mechanisms in mice with Parkinson disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). C57BL/6J mice were injected with MPTP for 8 days to establish a PD model. Nicotine was given for 10 days in the nicotine therapeutic group. Animals were examined behaviorally with the pole test and traction test. Tyrosine hydroxylase (TH) and γ-aminobutyric acid (GABA) were determined by using the immunocytochemistry (ICC) method. The ultrastructural changes of the caudate nucleus (CN) were observed under electron microscopy. The results showed that pretreatment with nicotine could improve the dyskinesia of PD mice markedly. Simultaneously, TH-positive (P<0.01) neurons and G... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2604049 Tue, 14 Jul 2009 10:55:03 +0100 2604049 http://www.medworm.com/index.php?rid=2596198&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu6657131mv2561m7%2F Abstract  Renal tubulointerstitial fibrosis (TIF) is the common end stage of various chronic renal diseases, and pirfenidone (PFD) is a novel, broad-spectrum anti-fibrotic compound but little is known about its effect and mechanism of action on renal TIF. In this work, we employed a unilateral ureteral obstruction (UUO) rat model to investigate the apoptosis of renal tubular epithelial cells (RTC) after PFD treatment. Thirty-five Sprague Dawley (SD) rats were randomized into three groups: sham-operated group (n = 7), UUO group (n = 14) and PFD group (n = 14). All rats were sacrificed at day 7 or 14 after operation. Renal histology was studied by using periodic acid schiff reagent (PAS) and Masson trichromic stain (MASSON); apoptosis was detected by in situ terminal deoxynucl... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2596198 Sat, 11 Jul 2009 07:36:55 +0100 2596198 http://www.medworm.com/index.php?rid=2589974&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd7071rr1686814r5%2F Abstract  Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×106 and 4.096×106, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients in... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2589974 Mon, 06 Jul 2009 20:04:35 +0100 2589974 http://www.medworm.com/index.php?rid=2558099&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1344414037565366%2F Abstract  A referral patient who had previously undergone varicose vein surgery was admitted as an emergency case. On admission, the patient complained of intolerable pain, paralysis and paresthesia of the affected limb, which was characterized by acute arterial ischemia symptoms. Color Doppler of the artery of the affected limb indicated that no blood flow signal existed in the superficial femoral artery. During exploratory operation, we found that the right superficial femoral artery instead of the great saphenous vein of the affected limb had been stripped and ligated. Therefore, the intact right great saphenous vein was taken for auto-transplantation by inverse end-to-end anastomosis to the proximal and distal residual superficial femoral artery, which resulted in gradua... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2558099 Mon, 29 Jun 2009 08:54:00 +0100 2558099 http://www.medworm.com/index.php?rid=2549677&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm248r84h63163427%2F Abstract  The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in oral squamous cell carcinoma (OSCC) and tongue cancer cell line Tca8113, as well as their effects on each other. We detected PTTG protein and bFGF in OSCC tissues from 56 cases using the streptavidin-biotin peroxidase (S-P) method; additionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expression in Tca8113 was examined by immuno-cytochemistry. The results were as follows: (1) Positive rates of PTTG protein and bFGF were 78.2% and 67.3% in OSCC, respectively, which were significantly higher than those in normal mucosal tissues (P<0.05). PTTG protein was significan... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2549677 Fri, 26 Jun 2009 09:57:22 +0100 2549677 http://www.medworm.com/index.php?rid=2408273&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl5u162435488v7t2%2F Abstract  The study aimed to detect the expression of the Th1-specific cell surface protein T cell Ig and mucin domain-containing molecule-3 (Tim-3) mRNA in peripheral blood lymphocytes isolated from asthmatic patients and to examine the correlation among Tim-3 mRNA, interleukin-4 (IL-4), interferon-γ (IFN-γ) level, and FEV1/FVC (force expiratory volume in one second/forced vital capacity) to explore the role of Tim-3 in the development and progression of asthmatic inflammation. Tim-3 mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The IL-4 and IFN-γ levels were determined by using enzyme-linked immunosorbent assay (ELISA). The correlation among Tim-3 mRNA, IL-4, IFN-γ level, and pulmonary ventilatory capacity was analyzed. The ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2408273 Sat, 09 May 2009 05:54:27 +0100 2408273 http://www.medworm.com/index.php?rid=2371467&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj2g6g79300801655%2F This study aimed to investigate the expression of protease-activated receptors (PARs) on platelets in healthy individuals and preliminarily elucidate physiological functions of PARs. Thirty healthy volunteers, who did not take any anticoagulants and antiplatelet agents within 10 days before the examination, were recruited. Fasting venous blood (5 mL) was taken from the medial cubital vein in each individual and platelet-rich plasma (PRP) was prepared. The expression of PAR1 mRNA and PAR4 mRNA in PRP was determined by RT-PCR analysis. The results showed that the average levels of PAR1 mRNA and PAR4 mRNA on platelets in healthy individuals were 0.1601 ± 0.0269 and 0.1073 ± 0.0194 respectively. In combination with literature analysis, it was concluded that the thrombin signaling pathw... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371467 Sun, 26 Apr 2009 05:52:04 +0100 2371467 http://www.medworm.com/index.php?rid=2371468&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr1mk246559j37812%2F Abstract  The mRNA and protein expression of phosphatase of regenerating liver 1 (PRL-1) and phosphatase of regenerating liver 3 (PRL-3) in transitional cell carcinoma of bladder (BTCC) and normal epithelia of bladder was investigated, and the relationship between the BTCC and pathological changes was clarified. The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) in 30 cases of BTCC and 10 cases of normal bladder, and the expression of PRL-1 and PRL-3 protein was checked by using immunohistochemistry in 30 cases of BTCC and 15 cases of normal bladder. The expression levels of PRL-1 and PRL-3 mRNA and protein were higher in BTCC than those in normal bladder epithelia (P<0.05). The increased expression of PRL... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371468 Sun, 26 Apr 2009 05:52:03 +0100 2371468 http://www.medworm.com/index.php?rid=2371470&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1754019v6988gmnt%2F Abstract  To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2 (Adanti-ERK2) gene therapy upon chronic allograft nephropathy (CAN) of rats, male Lewis (LEW, RT11) rats received male Fisher (F344, RT11v1) renal allografts. The recipients were divided into three groups: (1) empty control group; (2) vector control group; (3) gene therapy group. All recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation. Morphometric analysis was used to determine the fibrosis of grafts. Immunohistochemistry was used to detect the expression of E-Cadherin, Vimentin, TβR I and the infiltration of CD4+ T lymphocyte, CD8+ T lymphocyte and ED-1+ monocytes. Enzyme linked immunosorbent assay (ELISA) was used to detect TG... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371470 Sun, 26 Apr 2009 05:52:02 +0100 2371470 http://www.medworm.com/index.php?rid=2371469&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F98054g13h3q7j31u%2F Abstract  Rab proteins and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome to trans-Golgi-network trafficking. To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed lentivirus encoding Rab9 cDNA. The expressing plasmid pCDH1-MCF1-Rab9-EF1-copGFP was constructed by using molecular biological techniques. The lentivirus encoding Rab9 cDNA was packed by Lifectamine-2000 mediated co-transfection of the plasmid pPACKH1-GAG, pPACKH1-REV and pVSV-G into 293T cells. DNA sequencing proved the successful construction of pCDH1-MCF1-Rab9-EF1-copGFP. After 72 h, the expression of GFP could be detected in BV-2 cells. Western blotting revealed that the Rab9... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371469 Sun, 26 Apr 2009 05:52:02 +0100 2371469 http://www.medworm.com/index.php?rid=2366347&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn543p01518023512%2F Abstract  Our previous studies showed that there were close relationships between connexin 43 (Cx43) and acupoints and meridians. In order to further investigate the effect of Cx43 in acupuncture treatment, RNA interference technique was used to construct small hairpin RNA (shRNA) expression vectors targeting Cx43 and identify the efficiency of RNA interference in NIH/3T3 cell lines for further use in vivo. Aiming directly at the two targets of Cx43 mRNA sequence of the rat and mouse homology region, we synthesized two pairs of complementary oligonucleotide strands in vitro. Double strands were formed after annealing, and then inserted into Pgenesil-1 plasmid expression vector. After identification by enzyme cutting and sequencing, the recombinant plasmids named P-Cx43-shRNA... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2366347 Thu, 23 Apr 2009 08:04:47 +0100 2366347 http://www.medworm.com/index.php?rid=2358119&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl76t151q42647ur5%2F Abstract  To clarify the effect of DNA methylation and histone deacetylase inhibitors on the expression of the E-cadherin gene and the invasiveness of the QBC939 cells, the QBC939 cells were separately treated with hydralazine, valproate, or combination of the two drugs. The expression of E-cadherin mRNA and protein was examined by reverse transcription-polymerase chain reaction (RT-PCR), and Western blot.The methylation status of the promoter region of the gene was detected by methylation-specific PCR (MSP). The invasiveness of QBC939 cells was tested by transwell assay. It was found that the promoter region of the E-cadherin gene of QBC939 cells was hypermethylated. Valproate alone could not contribute to demethylation of the gene, whereas hydralazine could make them to be ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358119 Tue, 21 Apr 2009 10:48:33 +0100 2358119 http://www.medworm.com/index.php?rid=2491596&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk271h6185k4377n3%2F This study indicates that EMT and expressions of VEGF, MMP-9 and COX-2, and MVD value are strongly correlated with lymph node metastasis in breast cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0038-1Authors Lihui Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaLianhong Li, Dalian Medical University Department of Pathology Dalian 116044 ChinaShen Lv, The Second Affiliated Hospital of Dalian Medical University Center Laboratory Dalian 116027 ChinaShujun Fan, Dalian Medical University Department of Pathology Dalian 116044 ChinaLi Zhan, Dalian Maternity Hospital Department of Pathology Dalian 116012 ChinaBo Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaZhong Zhang, Dalian Medical University Depa... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2491596 Tue, 21 Apr 2009 10:48:32 +0100 2491596 http://www.medworm.com/index.php?rid=2358133&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk271h6185k4377n3%2F This study indicates that EMT and expressions of VEGF, MMP-9 and COX-2, and MVD value are strongly correlated with lymph node metastasis in breast cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0038-1Authors Lihui Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaLianhong Li, Dalian Medical University Department of Pathology Dalian 116044 ChinaShen Lv, The Second Affiliated Hospital of Dalian Medical University Center Laboratory Dalian 116027 ChinaShujun Fan, Dalian Medical University Department of Pathology Dalian 116044 ChinaLi Zhan, Dalian Maternity Hospital Department of Pathology Dalian 116012 ChinaBo Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaZhong Zhang, Dalian Medical University Depa... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358133 Tue, 21 Apr 2009 10:48:32 +0100 2358133 http://www.medworm.com/index.php?rid=2358126&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu011m1w4279503x7%2F In this study, 100 women who were enrolled were randomly divided into two groups. In one group, the TCT for cervical cells was applied (TCT group), and in the other group routine cervical smear was used. In addition, the cells in the TCT group were screened by double sifters, and centrifuged using a separation medium so as to eliminate mucus, inflammatory cells and blood cells. According to the cell distribution and the thickness of the smear, the results were assigned to three groups, including satisfactory smears, less satisfactory smears and unsatisfactory smears. The TCT had a higher satisfactory rate (98%) compared to the routine cervical smear (32%) (P<0.01), indicating the TCT was superior to the routine cervical smear. It is concluded that the TCT is more acceptable. Meanwh... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358126 Tue, 21 Apr 2009 10:48:32 +0100 2358126 http://www.medworm.com/index.php?rid=2358147&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa643776235m438t4%2F Abstract  The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associated virus (AAV) in vitro was investigated. Recombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinant AAV genome was replicated and packaged with the helper virus HSV1-rc/ΔUL2. The titer of the recombinant rAAV2-tet-off-bFGF was determined by dot-blot assay. MC3T3-E1 cells were infected with rAAV2-tet-off-bFGF. Regulatory effects of Doxycycline (Dox) on bFGF and osteogenic factors were assayed quantitatively by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The physical particle titer of rAAV2-tet-off-bFGF successfully constructed was 1.8×1012 vector genomes/mL, and the virus could i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358147 Tue, 21 Apr 2009 10:48:30 +0100 2358147 http://www.medworm.com/index.php?rid=2358140&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj2585g964gj773w1%2F The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0–100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions (P < 0.05 vs control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation (P < 0.05 vs control) but had no effect on NO pr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358140 Tue, 21 Apr 2009 10:48:30 +0100 2358140 http://www.medworm.com/index.php?rid=2358154&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh1764433m3l5164l%2F In conclusion, single ovarian stimulation may produce more oocytes/embryos. However, repeated gonadotropin stimulation may have a negative effect on the ovarian follicular quality, the number of mature retrieved oocytes, and the embryo quality, even increasing the chance of ovarian cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0037-2Authors Linlin Liang, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College Wuhan 430030 ChinaBei Xu, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College Wuhan 430030 ChinaGuijin Zhu, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358154 Tue, 21 Apr 2009 10:48:28 +0100 2358154 http://www.medworm.com/index.php?rid=2334700&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm37557727443g540%2F Abstract  The purpose of this study was to investigate the expression of inducible nitric oxide synthase(iNOS) in trophoblasts and deciduas in early medical abortion, and study the relationship of medical abortion through mifepristone and iNOS in early pregnancy. Expression of iNOS in trophoblasts and deciduas was detected by both in situ hybridization and immunohistochemical assay in 40 patients (experimental group); the positive expression of iNOS was represented by number density (N/S) and positive unit (Pu) using computer color magic image analysis system (CMIAS). All results were compared with those obtained from vacuum aspiration. In the experimental group, N/S and Pu in trophoblasts were 0.120±0.010 and 15.3±2.6, respectively, while in the control group, they were 0... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2334700 Tue, 14 Apr 2009 06:13:54 +0100 2334700 http://www.medworm.com/index.php?rid=2334701&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd340600068417055%2F Abstract  Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormone deficiency. To simplify the injection process and increase drug compliance, application of the GH injection has become a new treatment plan in recent years. The purpose of the current study was to evaluate the efficacy and safety of rhGH injection for the treatment of growth hormone deficiency (GHD) in children in China. In a nationwide, noncomparative, prospective, randomized, open trial, 31 children with confirmed complete GHD received subcutaneous injection of rhGH at 0.25 mg/kg·wk (0.107 IU/kg·d). The injection was given daily and the total weekly amount was separated into 6–7 injections. The patients were followed up at 3-month intervals and the treatm... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2334701 Tue, 14 Apr 2009 06:13:53 +0100 2334701 http://www.medworm.com/index.php?rid=2311212&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr48hh3j5784m173w%2F Abstract  We implemented a new protocol—multiphase dynamic helical scan to acquire CT angiography (CTA) and whole brain CT perfusion (CTP) images simultaneously with single scan on 16 multidetector CT (MDCT). A total of 90 patients who were randomly assigned into 3 groups were included in our study. Each group underwent CT scan by using the new protocol, traditional CTA and CTP protocol, respectively. The image quality of CTA, the CTP parameter values and the X-ray doses were measured and compared between the new protocol and the traditional protocols. There was no statistically significant difference in the CTA image quality between the above methods (P = 0.55). For CTP parameters, the new protocol tended to overestimate the blood volume (BV) and blood flow (BF) value, an... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311212 Fri, 03 Apr 2009 06:48:43 +0100 2311212 http://www.medworm.com/index.php?rid=2311214&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg41915j84r22143p%2F Abstract  This retrospective study was performed to compare the outcome of thoracoabdominal incision versus flank incision for radical nephrectomy in the patients with large renal tumors. A questionnaire assessing postoperative pain, administration of pain medications and the return to activities and work was sent to the patients who underwent radical nephrectomy through the 11th rib (group 1: undergoing flank incision, including 96 patients) or the 9th to 10th rib (group 2: undergoing thoracoabdominal incision, including 98 patients) from 2003 to 2007 in our hospital. A case retrospective analysis assessing operation time, perioperative hemorrhage volume, size of tumor, success in the treatment of tumor thrombus in renal vein or vena cava, time length of presence of drainag... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311214 Fri, 03 Apr 2009 06:48:42 +0100 2311214 http://www.medworm.com/index.php?rid=2311218&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy77x1p3851547876%2F Abstract  In order to provide a sensitive cell line model for investigating the mechanisms underlying the lymphatic metastasis of tumors and the effect of medicine against cells, a new murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L6) was established and the effect of curcumin on biological behavior of Hca-P/L6 was observed. Murine ascites hepatocarcinoma cell strain with low lymphatic metastatic potential (Hca-P) was subcutaneously inoculated into the medioventral line of a mouse 615 and the first generation of metastatic tumor cells of inguinal lymph node (Hca-P/L1) was obtained. Then, Hca-P/L1 was screened by the route of mouse foot pad subcutaneously → lymph node → scale-up culture in vitro → mouse foot pad subcutaneously f... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311218 Fri, 03 Apr 2009 06:48:41 +0100 2311218 http://www.medworm.com/index.php?rid=2311216&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F056482m487770543%2F Abstract  To gain a broader appreciation of the clinical presentation, operative treatment, and outcome of fibrous dysplasia involving the calvarium in children, we retrospectively reviewed a series of cases of fibrous dysplasia involving the calvarium (4 males and 2 females) with patients’ age ranging from 5 to 12 years old. The clinical manifestation, radiographic findings, surgical treatment, outcome and follow-up were evaluated on the basis of medical records. Fibrous dysplasia in the series was monostotic, involving frontal bone (2 cases), temporal bone (1 case), parietal bone (2 cases) and occipital bone (1 case). The patients most commonly presented with enlarging mass and cosmetic complaints. The treatment given, depending on clinical presentation, was simple biop... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311216 Fri, 03 Apr 2009 06:48:41 +0100 2311216 http://www.medworm.com/index.php?rid=2311215&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F862017334460j651%2F We reported a case of diffuse cavernous hemangioma involving both skin and muscles of the left lower limb and perineal region in an adolescent patient. The patient who had previously undergone a local hemangioma resection of the foot ultimately ended in superficial femoral artery ligation and supergenual thigh amputation of the left upper leg because of uncontrollable massive bleeding of anastomotic stoma. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s11684-009-0031-8Authors Yin Xia, Huazhong University of Science and Technology Department of Vascular Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaDan Shang, Huazhong University of Science and Technology Department of Vascular Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaQin Li,... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311215 Fri, 03 Apr 2009 06:48:41 +0100 2311215 http://www.medworm.com/index.php?rid=2311219&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7825l58757m4gr82%2F Abstract  To investigate the regulation of tumor suppressor XAF1 gene expression in digestive system cancers, we studied XAF1 gene promoter transcription activity and mRNA level in digestive system cancer cell lines (human hepatoma cell line HepG2, human colon cancer cell line LoVo, and human gastric cancer cell line AGS) and nontumor cell lines (human embryonic liver cell line L02 (L02 cells) and human embryonic kidney 293 cells [HEK293 cells]) as controls. 1395-bp-promoter fragment of XAF1 gene was amplified by polymerase chain reaction (PCR) and cloned into pGL3-basic vector and pEGFP-1 vector to assay its promoter transcription activity. The plasmids were transfected into a variety of cell lines by lipofectamine 2000. The promoter transcription activity was determined b... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311219 Fri, 03 Apr 2009 06:48:39 +0100 2311219 http://www.medworm.com/index.php?rid=2311221&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F63ln4853605010x6%2F Abstract  To evaluate the mechanism of vascular endothelial growth factor (VEGF) on the prevention of restenosis after angioplasty, the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells (VSMC) in vitro. The conditioned medium containing VEGF was collected 72 h after the infection. Then, the VSMC and human umbilical vein endothelial cells (HUVEC) were divided into control group, H2O2-treated group and H2O2+ VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium (WST-1) method, in situ nick end labeling (TUNEL) and flow cytometry (FCM). Compared with the control and H2O2+ VEGF-treated groups, the absorbance (A) value of HUVEC was decreased, and apoptosis of HUVEC was signifi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311221 Sun, 29 Mar 2009 05:53:13 +0100 2311221 http://www.medworm.com/index.php?rid=2311222&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm777tt658n4224n1%2F Abstract  The purpose of our research was to evaluate the efficacy, tolerance, and safety of oxcarbazepine (OXC) as monotherapy and add-on therapy for partial epilepsy. We carried out a prospective clinical follow-up trial at the Epilepsy Center of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Sixty-seven patients with partial epilepsy received OXC therapy. The patients were randomly divided into a monotherapy group and an add-on therapy group. We observed the efficacy and safety in the first three months and the following three months respectively, and compared them with each other. There was a significant difference in the decrease of seizure frequency between the two groups (P = 0.002). There was a significant difference in the pe... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311222 Sun, 29 Mar 2009 05:53:12 +0100 2311222 http://www.medworm.com/index.php?rid=2278670&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F15566430x2156411%2F In this study, survival fraction at 2Gy (SF2) values of eight human tumor cell lines (including four human cervical carcinoma cell lines HeLa, SiHa, C33A, Caski, three human breast carcinoma cell lines MCF-7, MDA-MB-231, MDA-MB-453, and one human lung carcinoma cell line A549) were acquired by clone formation assay, and western blot was applied to detect the expressions of Ku80, DNA-PKcs and ATM protein. The correlativity of protein expression with SF2 value was analyzed by Pearson linear correlation analysis. We found that the expression of the same protein in different cell lines and the expression of three proteins in the same cell line had a significant difference. The SF2 values were also different in eight tumor cell lines and there was a positive correlativity between the exp... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278670 Tue, 17 Mar 2009 07:02:51 +0100 2278670 http://www.medworm.com/index.php?rid=2278672&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg347566582500466%2F Abstract  Reactive oxygen species (ROS) are small molecule metabolites of oxygen that are prone to participate in redox reactions via their high reactivity. Intracellular ROS could be generated in reduced nicotinamide-adenine dinucleotidephosphate (NADPH) oxidase-dependent and/or NADPH oxidase-independent manners. Physiologically, ROS are involved in many signaling cascades that contribute to normal processes. One classical example is that ROS derived from the NADPH oxidase and released in neurotrophils are able to digest invading bacteria. Excessive ROS, however, contribute to pathogenesis of various human diseases including cancer, aging, dimentia and hypertension. As signaling messengers, ROS are able to oxidize many targets such as DNA, proteins and lipids, which may be ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278672 Tue, 17 Mar 2009 07:02:48 +0100 2278672 http://www.medworm.com/index.php?rid=2278678&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx6814u08v7t658g6%2F Abstract  The aim of this paper was to explore the relationship between energy metabolism and the meridian phenomenon. The manner of change of oxygen partial pressure in acupoints and no-acupoints of the urinary bladder meridian of goats was observed with a needle-type tissue oxygen tension sensor after the acupuncture effect was blocked by Ca2+ complexation with ethylenediamine tetraacetic acid (EDTA)-Na2. The results showed that: (1) the concentration of calcium ion in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01 or P<0.05); (2) the tissue oxygen tension in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01); (3) the oxygen partial pressure in acupoints decreased significantly after injection with EDTA-... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278678 Tue, 17 Mar 2009 07:02:47 +0100 2278678 http://www.medworm.com/index.php?rid=2278676&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff4501641662878r1%2F Abstract  To study the efficacy of Fuganling granula (FGL, 复肝灵颗粒) in treating mouse immunological hepatic injury that was caused by Bacille Calmette Guerin (BCG) and lipopolysaccharide (LPS), a total of 60 mice were adopted, among which, 50 mice were given intraperitoneal injection with BCG and LPS to establish an immunological liver injury model and then were randomly divided into 5 groups (10 mice/group): 4 groups received treatment of FGL orally at the doses of 100 mg/kg (high-dosage), 50 mg/kg (middle-dosage), 25 mg/kg (low-dosage) and bifendate orally at the dose of 80 mg/kg, respectively. One group was treated with distilled water orally. The remaining 10 mice were given distilled water intraperitoneally as the normal control group. T... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278676 Tue, 17 Mar 2009 07:02:47 +0100 2278676 http://www.medworm.com/index.php?rid=2278674&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy73rn7lr66088605%2F Abstract  The aim of this study was to explore the regulatory mechanism of retinoic acid (RA) on the TBX1 gene expression in myocardial cells. Ventricular cardiocytes were isolated from neonatal rats and cultured, and then treated with different concentrations of retinoic acid. The expression of Shh and Fgf8 at mRNA and protein levels in neonatal rat myocardial cells were measured by using RT-PCR and Western blot technique, respectively. There was basal expression of Shh and Fgf8 in the control group. When treated with 3 × 10−7 mol/L RA, we observed that the expression of Shh mRNA and protein in neonatal rat myocardial cells were up-regulated by 1.51 (P < 0.05) and 1.10 times (P < 0.05), respectively. In comparison with the control group, under the concentration of ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278674 Tue, 17 Mar 2009 07:02:47 +0100 2278674 http://www.medworm.com/index.php?rid=2278680&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn025p8004451k052%2F Abstract  The lacrimal endoscope is applied to the diagnosis and therapy of the disorders of the lacrimal system in combination with laser or micro drills under orthophoria. The changes of mucous membranes, the characteristics of pathological changes and the predilection sites of lacrimal system diseases were initially approached. One hundred and forty six pairs of eyes of 128 patients with lacrimal system disease were observed by the lacrimal endoscope in the Ophthalmology Department of Tongji Hospital from June 2006 to March 2007. The dynamic changes in mucous membranes, lesion sites, secreted substances and formation of membrane could be observed under orthophoria. Combined with laser or micro drill, the endoscope was applied to the therapy of lacrimal system disorders a... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278680 Tue, 17 Mar 2009 07:02:46 +0100 2278680 http://www.medworm.com/index.php?rid=2141458&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2x4771pp3j125613%2F Abstract  In order to provide useful information for effective control and clinical therapy of infection, the resistance status and the rate of carrying AmpC β-lactamase of Enterobacter cloacae (E. cloacae) were investigated. By VITEK (Bacterial automatic biochemical analyzer), the isolates of E. cloacae were identified and the drug resistance was measured. The AmpC enzyme was detected by the five-disk diffusion test. Antibiotic sensitivity test showed that the resistance effects of E. cloacae to cefazolin, cefoxitin and ampicillin were more serious, with resistant rates of 80.5%, 75.3% and 70.1%, respectively. However, it was more sensitive to Sulperazone (cefoperazone/sulbactam, 13.0%), amikacin (16.9%) and ciprofloxacin (19.5%). Meanwhile, the phenotype detection showed t... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2141458 Tue, 27 Jan 2009 07:43:19 +0100 2141458 http://www.medworm.com/index.php?rid=2141457&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj3446k5047x82687%2F Abstract  During normal metabolism, oxidative byproducts will inevitably generate and damage molecules thereby impairing their biological functions, including the aging process. Bushenkangshuai Tang (补肾抗衰方, BT) is a traditional Chinese medicine widely used for clinically treating premature ovarian failure. In the present study, BT administration at high concentrations significantly increased lifespan, slowed aging-related decline, and delayed accumulation of aging-related cellular damage in wild-type Caenorhabditis elegans. BT administration could further largely alleviate the aging defects induced by UV and oxidative stresses, and BT administration at different concentrations could largely rescue the aging defects in mev-1 mutant animals. The... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2141457 Tue, 27 Jan 2009 07:43:19 +0100 2141457 http://www.medworm.com/index.php?rid=2141460&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh27376r6g476u034%2F Abstract  Divalent metal transporter 1 (DMT1) is a ferrous iron import protein. The improper expression of DMT1 is involved in neurodegenerative diseases. In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without the iron response element (DMT1-IRE) and investigated its expression and function in the C6 glioma cell line. The DMT1-IRE gene, obtained by RT-PCR, was cloned into the shuttle plasmiding pAdTrack-CMV containing green fluorescent protein (GFP) reporter gene. Linearized plasmid pAdTrack-CMV-DMT1-IRE was subsequently co-transformed into Escherichia coli (E. coli) BJ5183 cells along with an adenoviral backbone plasmid pAdEasy-1 after digestion with Pme I. Pac I-digested pAdEasy1-DMT1-IRE was then transfected into E1-transformed hu... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2141460 Tue, 27 Jan 2009 07:43:17 +0100 2141460 http://www.medworm.com/index.php?rid=2141459&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhl46326k85866r11%2F This study aimed to investigate the effects of celecoxib, synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalenecarboxylicacid (CD437) and the combination of the two on cell proliferation, apoptosis, and cycle arrest of human malignant melanoma A375 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay (MTT assay) was applied to determine the anti-proliferative effects of the drugs on human malignant melanoma A375 cells. Flow cytometry was performed to investigate the influence of the drugs on cell cycle and cell apoptosis. Both celecoxib and CD437 could inhibit the growth of human malignant melanoma A375 cells in a dose-dependent manner. Celecoxib at 80 μmol/L inhibited proliferation, induced apoptosis and G2/M cell cycle arrest of human malignant me... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2141459 Tue, 27 Jan 2009 07:43:17 +0100 2141459 http://www.medworm.com/index.php?rid=2141461&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9312711q51308h60%2F Abstract  The aim of this study was to explore the expression of integrin-β1 in different stages of hepatic fibrosis and intervention of resveratrol as well as the way by which integrin-β1 promoted hepatic fibrosis. Hepatic fibrosis models of male Sprague Dawley (SD) rats were created and intragastric administration of resveratrol was given in low (40 mg/kg), middle (120 mg/kg) and high (200 mg/kg) dose groups. The expression of integrin-β1, transforming growth factor-β (TGF-β) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in different stages of hepatic fibrosis was detected by using RT-PCR. The expression of hexadecenoic acid (HA) and precollagen III (pc III) was assayed by radioimmunoassay. The expression of integrin-β1, TGF-β and TIMP-1 was determined in ea... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2141461 Tue, 27 Jan 2009 07:43:16 +0100 2141461 http://www.medworm.com/index.php?rid=2131437&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff01350456006l744%2F In this study, 4-1BBL was expressed in non-immune cells and non-tumor cells, and the role of 4-1BBL in lymphocyte activation and tumor suppression was investigated. The plasmid p4-1BBL containing the full length of mouse 4-1BBL cDNA sequence was constructed, and the plasmid was transfected into baby hamster kidney (BHK) cells and murine muscle cells by means of lipofectin-mediated or naked plasmid DNA injection into the muscle directly. The study demonstrated that the molecule 4-1BBL expressed by BHK cells in vitro could enhance the proliferation and cytotoxicity of lymphocytes, and it could increase the expression level of IL-2 and IFN-γ. The treatment with plasmid p4-1BBL in vivo revealed that the number of CD8+ Tcells in the peri-tumoral tissue increased markedly, and the growth r... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2131437 Fri, 23 Jan 2009 09:17:46 +0100 2131437 http://www.medworm.com/index.php?rid=2131438&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn182m8301g00n6q4%2F This study was aimed to explore the anterior cervical surgery methods to treat central cord syndrome without radiographic spinal fracture-dislocation (CCSWORFD), retrospectively analyze the cases of CCSWORFD, and evaluate the curative effect of anterior cervical surgery methods for CCSWORFD. Twenty four cases of CCSWORFD (19 males and 5 females), all suffering from cervical hyperextension injury, between 45–68 (average 59) years old, were operated on by anterior cervical surgery methods. Among these, 18 cases had been followed up for 6–24 (average 15) months; 18 cases, who had anterior decompression and plate fixation with titanium mesh bone grafting or iliac bone grafting achieved reliable effects based on the Japanese Orthopedics Association (JOA) evaluation (improved scores of... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2131438 Fri, 23 Jan 2009 09:17:45 +0100 2131438 http://www.medworm.com/index.php?rid=2131439&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fwg11qh675p378159%2F Abstract  The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I:6 cases, II:1 case, III:1 case; Campanacci I:6 cases, II:1 case, III:1 case; Enneking Staging G0T1−2M0, 5 cases, G1T1−2M0: 2 cases, G1T2M0: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (P < 0.01... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2131439 Fri, 23 Jan 2009 09:17:44 +0100 2131439 http://www.medworm.com/index.php?rid=2112329&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fyjk063ql67031806%2F This study aims to research the expression of spleen tyrosine kinase (Syk) in non-small cell lung cancer (NSCLC) and the relationship between Syk and clinicopathologic factors and p53. Immunohistochemistry was applied to detect the expression of Syk and p53 protein in 39 cases of NSCLC (23 cases of lung squamous cell cancer, 16 cases of lung adenocarcinoma) and tumor-surrounding normal lung tissues. The positive rate of Syk was 46.15% (18/39) and 100% (39/39) in NSCLC and tumorsurrounding normal lung tissues, respectively. The expression level of Syk in NSCLC was significantly lower than that in tumor-surrounding normal lung tissues (P = 0.000). The Syk expression was positively correlated with the p53 expression in NSCLC specimens (P = 0.025). There was no significant association bet... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2112329 Fri, 16 Jan 2009 13:34:22 +0100 2112329 http://www.medworm.com/index.php?rid=2112328&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F871526624570p868%2F Abstract  Protein phosphatase 2A (PP2A) is the predominant serine/threonine phosphatase in eukaryotic cells. In the brains of patients with Alzheimer’s disease (AD), decreased PP2A activities were observed, which is suggested to be involved in neuro.brillary tangle (NFT) formation, disturbed amyloid precursor protein (APP) secretion and neurodegeneration in AD brain. Based on our research and other previous findings, decreased PP2Ac level, decreased PP2A holoenzyme composition, increased level of PP2A inhibitors, increased PP2Ac Leu309 demethylation and Tyr307 phosphorylation underlie PP2A inactivation in AD. β-amyloid (Aβ) overproduction, estrogen deficiency and impaired homocysteine metabolism are the possible up-stream factors that inactivate PP2A in AD neurons. Furt... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2112328 Fri, 16 Jan 2009 13:34:22 +0100 2112328 http://www.medworm.com/index.php?rid=2112330&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff5473k256163201v%2F This study aims to investigate the influence of β-elemene on the secretion of angiotensin II (ANG II) and the expression of angiotensin receptor type 1 (AT1R) in hepatic stellate cells (HSCs). In vitro, HSC-T6 were cultured for 24 hours and then treated with different doses of β-elemene (2.5, 5 and 10 mg/L). A control group was also set up. The secretion of ANG II in the supernatant was detected by radioimmunoassay. The mRNA expression of AT1R at 4, 12 and 24 h after treatment was detected by reverse transcription-polymerase chain reaction (RT-PCR), respectively. The protein expression of AT1R was detected by western blot. At the 4th h, the ANG II secretion in the supernatant was significantly inhibited by 10 mg/L β-elemene compared with the control group (P<0.05), while 5.0 mg/... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2112330 Fri, 16 Jan 2009 13:34:21 +0100 2112330 http://www.medworm.com/index.php?rid=2112332&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd0mp043478n11844%2F Abstract  Lymphography is often used for the diagnosis of lymphatic metastasis in oncology. Determination of lymphatic metastasis is extremely important for accurate cancer staging, which may directly guide the clinical therapeutic scheme. In recent years, the technology of lymphography has developed rapidly on the basis of traditional lymphography, with the appearance of computed tomography (CT) lymphography, nuclear magnetic resonance (MR) lymphography, contrast-enhanced lymphosonography, and so on; the diagnostic accuracy has also been improved. The imaging principles and methods of these various technologies of lymphography are reviewed in this paper, and their applications and significance in oncology are also discussed in detail. Content Type Journal ArticleCategory... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2112332 Fri, 16 Jan 2009 13:34:15 +0100 2112332 http://www.medworm.com/index.php?rid=2112331&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd82w50587066472p%2F Abstract  Leptin resistance is a main mechanism of acquired childhood obesity, and the suppression of long form of leptin receptor (OBRb) gene expression in dietinduced obese rats indicates that the down-regulation of OBRb gene expression plays a pivotal role in the mechanism of leptin resistance. The aim of the present study was to construct the lentiviral RNA interference (RNAi) vector of rat OBRb gene and evaluate the effects of siRNA on silencing OBRb gene expression. The target sequence of siRNA-OBRb was designed, and the complementary DNA containing both sense and antisense oligonucleotides was synthesized. After phosphorylation and annealing, these double-stranded DNA was cloned to pRNA-lentivector-VGFP to construct pRNA-Lenti-OBRb-VGFP recombinants with U6-containin... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2112331 Fri, 16 Jan 2009 13:34:15 +0100 2112331 http://www.medworm.com/index.php?rid=2097995&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8057730930103175%2F Abstract  The role of high sensitivity C-reactive protein (hsCRP) in predicting prognosis after stroke in the Asian population has not been investigated.We hypothesized that elevated levels of hsCRP were associated with worsening prognosis after stroke in Chinese patients. Two hundred and ninety consecutive patients with first-onset stroke and 290 age- and gender-matched control subjects without any cerebrovascular disease were enrolled for study. Plasma hsCRP level was detected and subsequent vascular events and death were recorded in both groups over a 5-year period. Compared to control group, patients presenting with stroke had higher plasma hsCRP level (3.3 ± 3.8 vs 1.3 ± 2.2 mg/L, P<0.01). Furthermore, in the group of patients with stroke, the mean plasma hsCRP lev... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2097995 Sat, 10 Jan 2009 09:41:51 +0100 2097995 http://www.medworm.com/index.php?rid=2033382&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5644638262124343%2F This study was aimed to investigate the relationship between tHcy level and prognosis of first-onset stroke in Chinese people. One hundred ninety six patients with first-onset ischemic stroke and ninety-five patients with first-onset hemorrhagic stroke were enrolled in this study. The patients were divided into two groups in terms of tHcy level (< 18 μmol/L and ⩾ 18 μmol/L). The plasma tHcy level was detected by a high performance liquid chromatography method with fluorescence detection. All the patients underwent a 5-year follow-up. Survival analysis shows that the probability of death or new vascular events in the ischemic stroke patients with high tHcy level (⩾ 18 μmol/L) was significantly higher than that in the counterparts with lower tHcy level (< 18 μmol/L) (50.9... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033382 Wed, 10 Dec 2008 07:50:44 +0100 2033382 http://www.medworm.com/index.php?rid=2033381&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm8322521308560n9%2F Abstract  The aim of this article is to explore the effect of arsenic trioxide (As2O3) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expression variation of vascular endothelial growth factor (VEGF). The viability and apoptosis of U266 cells were observed by methylthiazolyl-tetrazolium (MTT) assay and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). The effect of As2O3 on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. We found that As2O3 could significantly inhibit the growth of U266 cells, and the concentration for 50% growth inhibition (IC50) was 2 μmol/L. After treatment with 2, 5, 10 μmol/... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033381 Wed, 10 Dec 2008 07:50:44 +0100 2033381 http://www.medworm.com/index.php?rid=2033380&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6346577282j4843w%2F Abstract  The postural balance is a important aspect of assessment in vestibular rehabilitation therapy. As the computerized dynamic posturography, the foam posturography performed by using foam in posturography is a useful measurement tool for postural balance. Then, the reliability of foam posturography is critical in clinic. To our knowledge, there is no report about the test-retest reliability of foam posturography in the patients with vertigo in China. The foam posturography was taken on the patients with vertigo in stable state and repeated in 3 to 5 days. In the two assessments of test and retest, all subjects stood under 4 sensory conditions, such as firm surface with eyes open (T1), firm surface with eyes closed (T2), foam surface with eyes open (T3) and foam surfa... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033380 Wed, 10 Dec 2008 07:50:44 +0100 2033380 http://www.medworm.com/index.php?rid=2033379&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn1731713l2660475%2F Abstract  The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4+ T cells. The pMSCV-Foxp3 retroviral vector encoding Foxp3 gene was transduced into the PT67 packaging cell line. Virus-containing supernatant was applied to differentiate CD4+CD25− T cells. The resulting cells were sorted with flow cytometry. The expressions of CD25, CD127, CTLA-4 and the proliferation of transfected T cells were examined. The effect of transfected CD4+ T cells on the proliferation and cytokine production of CD4+CD25− T cells was examined. Foxp3-gene transfected CD4+ T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4, but down-regulated CD127 expression. After transfectio... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033379 Wed, 10 Dec 2008 07:50:44 +0100 2033379 http://www.medworm.com/index.php?rid=2033386&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb1233ku5200k29u5%2F Abstract  This paper reflects on the advancements of clinical and scientific research in the field of burn surgery in China. It includes emergency care of massive burns, resuscitation, anti-infection, prevention and cure of internal organ injuries, metabolic and nutritional support, wound repair and rehabilitation, and special types of burns; it also covers pathology, microbiology, immunology, cell biology, molecular biology, and tissue engineering. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-008-0063-5Authors Shiliang Wang, the Third Military Medical University Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400038 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033386 Wed, 10 Dec 2008 07:50:43 +0100 2033386 http://www.medworm.com/index.php?rid=2033385&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu172tj20640g3308%2F Abstract  Organophosphates (OPs) and Pyrethroids (PRY) have been widely used in agriculture and in the home as broad spectrum insecticides, but may produce considerable risk to human health, especially to children. Children are more susceptible to environmental exposure, and concern about the neurotoxic effects of pesticide exposure on children is increasing. There is a need for better understanding of the potential developmental neurotoxicity of pesticides. Techniques for assessing developmental neurotoxicity of pesticides will continue to be developed, rendering a need for flexibility of testing paradigms. Current techniques used in evaluating the developmental neurotoxicity of OPs and PRY are presented in this review. These include: (1) In vitro techniques (PC12 cells, C6... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033385 Wed, 10 Dec 2008 07:50:43 +0100 2033385 http://www.medworm.com/index.php?rid=2033384&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F45881p075r00437g%2F Abstract  This paper reviews the present theories and empirical research of autisms’ cognitive research and mirror systems and introduces a new hypothesis about the causes of autism spectrum disorders (ASD): autistic mirror neuron dysfunction hypothesis. ASD subjects show obvious lack of the activation of the mirror system during the task of observation or emotional cognition. It is significant to investigate the mirror system for revealing the causes of autism and it is also helpful for developing new ways to diagnose or treat this disorder. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-008-0065-3Authors Wei Chen, Hangzhou Normal University Psychology Department Hangzhou 310018 ChinaJing Zhang, Hangzhou Normal University Psychology Department Hangzhou ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033384 Wed, 10 Dec 2008 07:50:43 +0100 2033384 http://www.medworm.com/index.php?rid=2033383&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl2461v8k23u2q007%2F Abstract  It has been found that lifestyle and diet are associated with the development of cancers. The mortality of breast cancer in Wuhan city is increasing, according to the statistics of recent years. This case control study was aimed to provide data for alimentary therapeutics for breast carcinomas. It included a case group (n = 196) and an age frequency-matched control group (n = 202). A validated food frequency questionnaire was used to obtain information on usual food consumption. The results indicate that intake of soy foods more than 5 times per week was associated with a decreased risk of breast cancer, especially in premenopausal women. The adjusted OR was 0.294 [95% CI was (0.158−0.546), P = 50.000]. Our results indicate that alimentary therapeutics for breast ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033383 Wed, 10 Dec 2008 07:50:43 +0100 2033383 http://www.medworm.com/index.php?rid=2033390&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1q82944mhp7807p1%2F Abstract  To investigate the significance of waist circumference (WC), body mass index (BMI) and hyperinsulinaemia A (HIns) in evaluating metabolic syndrome (MS). Clinical data from middle-aged and senile individuals (middle-senile group) who received glucose tolerance test after diabetes mellitus screening and a group of subjects who received annual oral glucose tolerance test (OGTT) for diabetes mellitus screening (adult group) were collected. Data were collected by use of special messengers, input into a computer data base and analyzed using SAS 5.0 software by expert staff. Abnormal WC and BMI were determined according to International Diabetes Federation (IDF) and Chinese Diabetes Society (CDS) criteria. HIns was ascertained if fasting insulin (FIns) ⩾ 15 mU/L, and/o... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033390 Wed, 10 Dec 2008 07:50:42 +0100 2033390 http://www.medworm.com/index.php?rid=2033389&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh753510132084031%2F This article is to explore if rTMS can cause changes of such chemical substances as N-acetylaspartate (NAA), choline (Cho) and creatine (Cr) in the surrounding area of experimental intracerebral hematoma of rabbits. A total of 36 rabbits were randomly divided into 3 groups: a control group (group A), a sham rTMS group (group B) and an rTMS group (group C). The experimental intracerebral hemorrhage (ICH) was induced by intracerebral injection of selfbody blood of the animals in groups B and C, while those of the group A serving as controls were injected with normal saline. The rabbits of groups B and C were treated with sham and real rTMS, respectively, but those of group A were not. The contents of chemical substances including NAA, Cho and Cr in the perihematomal brain tissues were ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033389 Wed, 10 Dec 2008 07:50:42 +0100 2033389 http://www.medworm.com/index.php?rid=2033388&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F23l4873276385318%2F Abstract  Substernal goiter is considered to be a diagnostic differential for all anterosuperior mediastinal masses. For a substernal goiter, surgical removal is suggested when it is large in size, with a possibility for malignancy or has local compression of adjacent structures. This can be performed through the neck or by the addition of a partial or complete sternotomy if necessary. A 58-year-old Chinese man from Guangdong Province had had dry cough for a year. A subsequent CT scan in our hospital suggested “ectopic intrathoracic thyroid”. Fine needle percutaneous mass biopsy also suggested “ectopic intrathoracic thyroid”. We performed a standard median sternotomy to remove the mass. It measured 13 cm × 8 cm × 6 cm in size, and weighed 2.8 kg. The pathological ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033388 Wed, 10 Dec 2008 07:50:42 +0100 2033388 http://www.medworm.com/index.php?rid=2033387&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj5k560118673k80m%2F Abstract  Hypertension is characterized by vascular smooth muscle constriction and vascular remodeling involving cell migration, hypertrophy and growth. Crk-associated substrate (CAS), the first discovered member of the adapter protein CAS family, has been shown to be a critical cellular component that regulates various smooth muscle functions. In this review, the molecular structure and protein interactions of the CAS family members are summarized. Evidence for the role of CAS in the regulation of vascular smooth muscle contractility is presented. Contraction stimulation induces CAS phosphorylation on Tyr-410 in arterial smooth muscle, creating the binding site for the Src homology (SH) 2/SH3 protein CrkII, which activates neuronal Wiskott-Aldrich syndrome protein (N-WASP)... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033387 Wed, 10 Dec 2008 07:50:42 +0100 2033387 http://www.medworm.com/index.php?rid=2033394&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy5811162638820v3%2F This study was designed to clarify the cellular characteristics in bronchoalveolar lavage fluid (BALF) and body weight loss of rats exposed to oxygen(> 90%). Young male Wistar rats, aged 6 weeks, were divided into three groups: (1) room air group (exposed to room air, n = 22); (2) hyperoxia < 48 h group (exposed to over 90% oxygen for less than 48 h, n = 18); (3) hyperoxia 66–72 h group (exposed to over 90% oxygen for 66–72 h group, n = 7). Compared to the room air group, the total cell counts in the hyperoxia 66–72 h group decreased, whereas the neutrophils increased significantly. The body weights of the rats exposed to room air continued to increase. However, the body weights of oxygen-exposed rats increased slightly on the first day and weight loss was seen from the sec... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033394 Wed, 10 Dec 2008 07:50:41 +0100 2033394 http://www.medworm.com/index.php?rid=2033393&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0mg8105275754748%2F In this study, the eukaryotic vector pshRNA-DNMT1 was constructed and transfected into T24 cells. Levels of DNMT1 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Relative to the blank control at the 24th, 48th and 72nd hour after transfection of pshRNA-DNMT1, the inhibitory rates of DNMT1 mRNA levels in T24 cells were 28.44%, 52.48%, 70.91%, respectively. Those of DNMT1 proteins were 24.27%, 57.79%, and 77.74%, respectively. Proliferation and apoptosis were assayed by MTT and flow cytometry with Annexin-V-FITC/PI staining. The growth inhibition rates of pshRNA-DNMT1 at the 24th, 48th and 72nd hour after transfection of pshRNA-DNMT1 were (4.34 ± 0.76)%, (9.87 ± 1.54)% and (13.78 ± 1.93)%, respectively. There were statisti... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033393 Wed, 10 Dec 2008 07:50:41 +0100 2033393 http://www.medworm.com/index.php?rid=2033392&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F80625182526241gq%2F Abstract  The aim of this paper is to investigate the safety of reconstructed adenovirus in immunosuppressive therapeutics and to explore the role of ciclosporin A in antagonizing the elimination of the vector. Several rats were given retroperitoneal injection of purified ADV-TK in order to obtain models. After 14 days’ treatment of ciclosporin A, samples of different periods were obtained, then stained with hematoxylin-eosin (HE) to detect inflammation reactions. Immunohistochemistry was used to examine the expression of adenovirus in organs. The results are as follows: (1) In HE stained sections of the organs, some transitory and reversible inflammation was detected. (2) In immunohistochemistry assay, reconstructed adenovirus decreased gradually as time went by in the c... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033392 Wed, 10 Dec 2008 07:50:41 +0100 2033392 http://www.medworm.com/index.php?rid=2033391&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg27131193x17jh05%2F Abstract  The aim of this paper is to investigate the clinical features and transformation of elderly male patients with normal blood glucose levels at fasting and 2 hours after glucose intake but with hyperglycemia (⩾ 11.1 mmol/L) 1 hour after oral glucose tolerance test (OGTT-1h HG). Seven years of follow-up visits were performed on 189 elderly male outpatients with OGTT-1h HG and data was recorded on their body mass index (BMI), blood pressure, serum cholesterol and triglyceride test results and on their glucose tolerance changes every 1–2 years after taking OGTT; their possible causes were analysed. Follow-up visits revealed that 19 patients with OGTT-1h HG were diagnosed with diabetes (10.1%), 78 patients with impaired glucose tolerance (IGT, 41.3%), 2 patients tra... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033391 Wed, 10 Dec 2008 07:50:41 +0100 2033391 http://www.medworm.com/index.php?rid=2033397&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F733g3h155t6n573p%2F Abstract  Brunner’s gland adenoma is a rare tumour of the duodenum, which is usually benign. A 71-year-old woman presenting with epigastric pain, upper gastrointestinal haemorrhage and melaena was reported in this paper. Upper gastro-intestinal (GI) endoscopy revealed a large pedunculated tumour on the superior part of the duodenal bulb. Endoscopic polypectomy was successfully performed by clipping and nylon thread without any complications. Histological examination revealed a Brunner’s gland adenoma. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s11684-008-0080-4Authors Binbin Zhang, Heilongjiang Provincial Hospital Department of Digestive Disease Harbin 150001 ChinaXu Ren, Heilongjiang Provincial Hospital Department of Digestive Disease Harbin 150001 C... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033397 Wed, 10 Dec 2008 07:50:40 +0100 2033397 http://www.medworm.com/index.php?rid=2033396&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff7227m545x1u6312%2F Abstract  To study the effects of early signal substances induced by heat stress in brains of Kunming mice, six-month-old mice (n = 72) were pretreated with heat stress and subsequent ischemia/reperfusion by clipping of their bilateral cervical common arteries for 7 min. According to different treatments, animals were randomly divided into four groups: (1) normal control group; (2) heat stress pretreatment followed by ischemia and reperfusion group (HS/IR); (3) ischemia and reperfusion group (IR); (4) heat stress group (HS). Animals in the later three groups were subdivided into 3 subgroups (1 day, 4 days, 14 days), respectively. The changes in the expression of cAMP response element binding protein (CREB) and calcitonin gene-related peptide (CGRP) were detected by immunohis... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033396 Wed, 10 Dec 2008 07:50:40 +0100 2033396 http://www.medworm.com/index.php?rid=2033395&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff38570447t748484%2F In conclusion, the abnormal expression of c-Myc was present in anti-BPDE malignantly transformed 16HBE cells, which may be involved in the carcinogenesis molecular mechanism of anti-BPDE. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-008-0073-3Authors Juan Fu, Tongji Medical College of Huazhong University of Science and Technology Department of Occupational and Environmental Health, the Ministry of Education Key Laboratory of Environment and Health, School of Public Health Wuhan 430030 ChinaYiguo Jiang, Guangzhou Medical College Institute for Chemical Carcinogenesis Guangzhou 510182 ChinaXuemin Chen, Tongji Medical College of Huazhong University of Science and Technology Department of Occupational and Environmental Health, the Ministry of Education Key Labor... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2033395 Wed, 10 Dec 2008 07:50:40 +0100 2033395 http://www.medworm.com/index.php?rid=1679508&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F061628701308412t%2F Abstract  Phthalate esters are widespread in the environment. They have been described as being one of the most abundant man-made environmental contaminants that may be adverse to human health. Particularly, di-2-ethylhexyl phthalate (DEHP) has been shown to cause reproductive and developmental toxicity and is suspected to be an endocrine disruptor. The primary objective of this study is to determine the estrogenic activity of DEHP. Estrogenic activities of DEHP were studied by in vitro assays of human breast cancer MCF-7 cell proliferation. Estrogen-dependent MCF-7 cells were grown in RPMI1640 medium containing 10% fetal bovine serum. Five days before the addition of the test compounds, the cells were washed by phosphate balanced solution (PBS), and the medium was substitut... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679508 Sat, 02 Aug 2008 07:05:26 +0100 1679508 http://www.medworm.com/index.php?rid=1679507&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8173757481564587%2F Abstract  The aim of this paper is to observe the effect of the Feixian Recipe on pulmonary fibrosis in rats. A rat model with pulmonary fibrosis was established by intratracheal injection of bleomycin. On days 14, 28 and 45, the contents of laminin, collagen I and collagen III in lung tissue homogenate in the model group, the sham operated group, the Feixian group and the prednisone group were measured. The contents of laminin and collagen I and III were decreased significantly by the Feixian Recipe. Feixian Recipe has a significant therapeutic effect on bleomycin-induced pulmonary fibrosis in rats. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-008-0060-8Authors Xiaomei Zhang, Beijing University of Chinese Medicine Department of Respiratory Dis... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679507 Sat, 02 Aug 2008 07:05:26 +0100 1679507 http://www.medworm.com/index.php?rid=1679509&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy75613mq575522g6%2F Abstract  Single-tube bi-directional allele specific amplification (SB-ASA) and real-time quantitative polymerase chain reaction (RQ-PCR) assays were developed and performed for JAK2V617F detection on 40 polycythemia vera (PV) samples, 31 essential thrombocythemia (ET) samples, 40 acute leukemia samples, and 40 healthy control samples. Differences between detect limitations of the two assays and their influence on the mutation detection rate were analyzed. The results showed that in some samples, the JAK2V617F burden was as low as nearly 1%, and thus more JAK2V617F-positive samples were detected by RQ-PCR than by SB-ASA assay due to the former higher detect limitation. Mutation allele ratios in PV and ET samples and their relevance to biological characteristics were also an... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679509 Sat, 02 Aug 2008 07:05:24 +0100 1679509 http://www.medworm.com/index.php?rid=1679510&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp322p310557634m1%2F Abstract  The aim of this paper is to investigate a method which may decrease the incidence and severity of oculocardiac reflex (OCR) without drugs. One hundred and sixty children undergoing strabismus surgery were allocated to two groups using double-blind randomization. OCR was defined as a decrease of more than 10% from the baseline heart rate during operation. An alarm sounded and a lamp flashed as soon as OCR occurred in group I, and neither of the above happened in group II. OCR occurred (1.151 ± 0.858) times in group I and (2.287 ± 1.371) times in group II (P < 0.05). Heart rate decreased by (23 ± 19) bpm in group I and (35 ± 28) bpm in group II (P < 0.05). The duration of OCR in group I and group II was (4.36 ± 4.26)s and (7.62 ± 6.41)s, respectively (P &l... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679510 Sat, 02 Aug 2008 07:05:23 +0100 1679510 http://www.medworm.com/index.php?rid=1679512&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7001670035p4p761%2F Abstract  The aim of this paper is to report a new coding variance of the TNFRSF9 gene, a candidate for auto-immune diseases. We found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening method and confirmed our results by direct sequencing and PCR-RFLP. Although without changing the amino acid coding, the variance may have an effect on codon usage and play a role in disease development, such as type 2 diabetes mellitus. However, we cannot define the role of this variance because the frequency of the minor allele is low in the Chinese population and no homozygote of the variance was found. More research in multiple populations will be necessary to define the role of this variance. Content Type Journal ArticleCategory Research ArticleDO... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679512 Sat, 02 Aug 2008 07:05:22 +0100 1679512 http://www.medworm.com/index.php?rid=1679511&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ffx42059j4011px0m%2F Abstract  Idiopathic nephrotic syndrome (INS) is characterized by marked urinary excretion of albumin and other intermediate-sized plasma proteins, such as transferrin and vitamin D-binding protein. Some cases even develop anemia. The aim of this study was to investigate the changes in serum iron, transferrin, and erythropoietin, and the relationships between serum and urine transferrin and erythropoietin. Thirty-seven children with INS and 35 age-and sex-matched healthy children were investigated. The indexes related to iron metabolism, including serum iron, ferritin, transferrin, total iron-binding capacity (TIBC), transferrin saturation, and hematological parameters [hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH)], and urinary transferr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679511 Sat, 02 Aug 2008 07:05:22 +0100 1679511 http://www.medworm.com/index.php?rid=1679513&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxv06855g7n240762%2F In this study, the serum levels, including thyroid hormones free triiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating hormone(TSH)among the subjects from the exposed group (n = 48) and the control group (n = 45) were detected by immuno radiometric assay (IRMA). The expression levels of TRα1, TRβ1, TSHR mRNA in placentas and umbilical cords were detected by fluorescent quantitative real-time PCR (FQ-PCR). The correlations between the thyroid hormone levels in maternal serum and umbilical serum, and between the expression levels of its receptors mRNA in placentas and umbilical cords were determined. We found that the FT4 levels of both maternal serum and umbilical cord serum in the exposed group were lower than those in the control (P < 0.05). However, the increased TSH ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679513 Sat, 02 Aug 2008 07:05:21 +0100 1679513 http://www.medworm.com/index.php?rid=1679515&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe1401711k6243711%2F Abstract  Meta-analysis was used to determine whether maintenance intravesical bacillus Calmette-Guerin (BCG) could reduce recurrence after transurethral resection of tumor 1 grade 3 (T1G3) superficial bladder cancer. All available published data of randomized clinical trials comparing transurethral resection plus intravesical BCG to either resection alone or resection plus another treatment on the treatment results in patients with superficial bladder cancer of T1G3 were selected for analysis. Both the fixed effects model and random effects model were applied, and the odds ratio (OR) with its 95% confidence interval (CI) was used as the effect size estimate. Sensitivity analysis and publication bias determination were performed by funnel plots and comparing ORs of different... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679515 Sat, 02 Aug 2008 07:05:20 +0100 1679515 http://www.medworm.com/index.php?rid=1679514&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn0q258325155t476%2F Abstract  The aim of this paper is to develop an applicable Random Amplified Polymorphic DNAs (RAPD) method for genotyping Neisseria gonorrhoeae strain, and discuss the possibility of using the RAPD method to trace N. gonorrhoeae strain transmission route. Four different pretreatment methods were used on the N. gonorrhoeae genomic DNA component, and the best adaptive extract method was selected for RAPD. Different RAPD primers sequence was used for amplification and their differentiating capabilities for N. gonorrhoeae strains were compared. Applicable RAPD primer was selected for N. gonorrhoeae genotyping and then applied into transmission detection. The results show that the so called cetyl-trimethylammonium bromide (CTAB) method for extracting genomic DNA could give integrat... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679514 Sat, 02 Aug 2008 07:05:20 +0100 1679514 http://www.medworm.com/index.php?rid=1679516&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6337j62k041302q6%2F Abstract  Domestic application of infrared patch clamp techniques on brain slices is limited. The key of the technique is to prepare high-quality brain slices. The present paper describes the preparation procedure of brainstem slices and the spontaneous firing properties of rat medial vestibular nucleus (MVN) neurons. By infrared differential interference contrast technique, neurons of rat MVN were visualized directly at the depth of 50–100 μm underneath the surface of slices. Firing activities of MVN neurons were recorded by the whole-cell patch clamp technique in artificial cerebrospinal fluid (ACSF) and low Ca2+-high Mg2+ fluid. The firing mode was more irregular and depressive in low Ca2+-high Mg2+ fluid than in ACSF. According to the averaged waveform of action potent... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679516 Sat, 02 Aug 2008 07:05:19 +0100 1679516 http://www.medworm.com/index.php?rid=1679518&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmw36551287363237%2F Abstract  The aim of this paper is to investigate the mechanism of hepatocellular damage in rats caused by low serum selenium. Thirty six rats were randomly divided into 2 groups: group A (fed with low-selenium diet from the Keshan Disease area with the content of selenium being 0.017 mg/kg); group B [fed with selenium-supplemented diet and 0.3 mg/L selenium (Na2SeO3) was added to the drinking water]. Both were respectively fed for 12 weeks. At the end of the 12th week, the levels of serum selenium, glutathione peroxidase (GPX) and malondialdehyde (MDA) in hepatic tissue were measured; the hepatocellular ultrastructure and apoptosis were observed as well. The levels of serum selenium and GPX in group A were markedly lower than those in group B. MDA level in group A was signif... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679518 Sat, 02 Aug 2008 07:05:17 +0100 1679518 http://www.medworm.com/index.php?rid=1679517&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw63t273j58337j67%2F Abstract  The vascular reactivity and calcium sensitivity were decreased following hemorrhagic shock. Arginine vasopressin (AVP) was beneficial to endotoxic, infectious/spetic and hemorrhagic shock. Our previous studies found that Rho kinase played an important role in the occurrence of calcium desensitization following shock. It was reported that AVP was with stimulation effect of Rho kinase. So we hypothesized that AVP might have beneficial effect on shock via activation of Rho kinase to regulate the calcium sensitivity and vascular reactivity. Hemorrhagic shock (40 mmHg for 2 h) Wistar rats in vivo were adopted to observe the effects of small dose of AVP on hemodynamics, 24-h survival rate, the pressor effect of norepinephrine (NE) and the contractility of superior mesent... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679517 Sat, 02 Aug 2008 07:05:17 +0100 1679517 http://www.medworm.com/index.php?rid=1679519&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd3607374wn0q3547%2F This study aimed to evaluate effects on hemodynamics in the portal venous system after CP and PCDV only. In 20 patients who received CP and 18 who received PCDV, hemodynamic parameters of the portal venous system were studied by magnetic resonance angiography 1 week before and 2 weeks after operation. Free portal pressure (FPP) was continuously detected by a transducer during the operations. Compared to the preoperative data, a decreased flow in the portal vein (PVF) [(563.12 ± 206.42) mL/min vs (1080.63 ± 352.85) mL/min, P < 0.05], a decreased portal vein diameter (PVD) [(1.20 ± 0.11) cm vs (1.30 ± 0.16) cm, P < 0.01], a decreased FPP [(21.50 ± 2.67) mmHg vs (29.88 ± 2.30) mmHg, P < 0.01] and an increased flow in the superior mesenteric vein (SMVF) [(1105.45 ± 309.03) ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679519 Sat, 02 Aug 2008 07:05:16 +0100 1679519 http://www.medworm.com/index.php?rid=1679520&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F50vv7q35163jw135%2F In this study, four commonly used housekeeping genes, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin, 28S rRNA and 18S rRNA were selected to test whether this assumption is tenable under hypoxic conditions. We tested the RNA expression level of these four genes in different hypoxic conditions. Rats subjected to acute hypoxia for 2 hours were used for tissue detection. Primary cultured neural stem cells from E13 fetal rats were treated with 3% O2 or 10% O2 for 24 hours for in vitro experiments. In both experiments, expression levels of 28S rRNA and 18S rRNA were constant, independent of hypoxia types. However, expression levels of GAPDH and β-actin were all changed in all kinds of hypoxic conditions. In particular, the mRNA expression level of GAPDH was increased by 43.4%... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679520 Sat, 02 Aug 2008 07:05:15 +0100 1679520 http://www.medworm.com/index.php?rid=1679522&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl8jk08438r772606%2F Abstract  Tissue engineering heart valves (TEHV) may be the most promising valve substitute, but the study has been relatively stagnant in the recent five years due to the special position, function and mechanical property of heart valves. It is one of the key factors to select an ideal scaffold material in the construction of TEHV. And this article will briefly review the current research and progress on the scaffolds of TEHV, especially based on Chinese works. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-008-0043-9Authors Nianguo Dong, Huazhong University of Science and Technology Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaJiawei Shi, Huazhong University of Science and Technology Department of Cardiova... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679522 Sat, 02 Aug 2008 07:05:14 +0100 1679522 http://www.medworm.com/index.php?rid=1679521&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv71498434521gm85%2F Abstract  The aim of this study is to investigate the effect of tyrosine kinase Src on Tyrosine 307(Y307) phosphorylation, protein phosphatase 2A (PP2A) activity, and on tau phosphorylation. Specific Src siRNA was transfected into cultured mouse neuroblastoma N2a cells to inhibit the expression of Src protein, and the phosphorylation levels of PP2A Y307 and tau at different sites, as well as PP2A activity were detected at different time points after siRNA transfection. Twelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreased PP2A Y307 phosphorylation. However, the total PP2A protein level was also decreased, together with a decreased PP2A activity. Tau was hyperphosphorylated at the Ser198/199/202 sites. Multiple factors may... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679521 Sat, 02 Aug 2008 07:05:14 +0100 1679521 http://www.medworm.com/index.php?rid=1679524&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F445498r1361k7033%2F Abstract  Wnt/β-catenin signaling pathway has been identified as a key cellular pathway in embryogenesis and disease, including cancers. In recent years, more and more interacting components have been observed and their exact functions approached, thus ensuring the most complicated understanding of this pathway in normal organism development and disorders. In hepatocellular carcinoma (HCC), with a deeply understanding of this pathway, more and more genes which contribute to aberrant activation of Wnt/β-catenin signaling pathway has recently been identified and their exact roles in HCC pursued. In this review, we will focus on a mostly updated understanding of this pathway and its observed role in HCC by emphasizing the gene defects identified to promote tumorigenesis and ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679524 Sat, 02 Aug 2008 07:05:12 +0100 1679524 http://www.medworm.com/index.php?rid=1679523&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc7j4h75832625652%2F Abstract  Hepatocellular carcinoma (HCC) represents an extremely poor prognostic cancer, which is mainly due to the high frequency of metastasis/recurrence after surgical operation. Exploring the molecular mechanisms involved in HCC metastasis could be helpful in the prediction and early diagnosis of HCC recurrence and could also provide new therapeutic targets for HCC metastasis. In the recent decade, we analyzed the genomic aberrations of the clinical specimens, as well as the metastatic models and cell lines of human HCC to identify the genetic markers related to HCC metastasis and to verify their clinical values in the prediction and control of metastasis of HCC. Using the comparative genomic hybridization (CGH) technique, we compared the differences of chromosomal aber... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679523 Sat, 02 Aug 2008 07:05:12 +0100 1679523 http://www.medworm.com/index.php?rid=1679526&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk208p533137h2438%2F This study demonstrates that ox-LDL can cause foam cell formation from macrophages in vitro. LPS can significantly accelerate this event. IL-10, an anti-inflammatory cytokine, can inhibit the effect of ox-LDL and LPS. These results indicate that inflammatory effects in blood vessels can speed up foam cell formation. The inhibitive effect of IL-10 is an important factor for delaying atherosclerosis processes. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-008-0057-3Authors Fei Wang, Medical College of Tongji University Department of Immunology Shanghai 200092 ChinaYalei Dai, Medical College of Tongji University Department of Immunology Shanghai 200092 ChinaTing Xu, Medical College of Tongji University Department of Immunology Shanghai 200092 ChinaBo Xu, Medi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679526 Sat, 02 Aug 2008 07:05:10 +0100 1679526 http://www.medworm.com/index.php?rid=1679525&cid=s_35962_22_f&fid=35962&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu4646hr8m33t2448%2F Abstract  The clinical therapy for cyclosporine A (CsA)-induced gingival hyperplasia (GH) and the pathological changes in hyperplastic gingival tissues were investigated. Nine cases of CsA-induced GH after renal transplantation were subjected to periodontal non-surgical treatment and surgical treatment and were followed up. Gingival index (GI), dental plaque index (PLI) and GH degree (GHD) at different time points were measured. Under light and electron microscopy, the pathological changes in hyperplastic gingival tissues were observed. The CsA concentrations in gingival tissues and blood were determined by using fluorescence polarization immunoassay with TDxFLx monoclonal antibody. The results showed that GI, PLI and GHD in patients with GH declined after periodontal treatm... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=1679525 Sat, 02 Aug 2008 07:05:10 +0100 1679525