MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Frontiers of Medicine in China' source. Fri, 04 Feb 2011 18:08:31 +0100 http://www.springerlink.com/content/062747x7g707g27r/ Abstract  The protective ability of host defense system is largely dependent on germ-line encoded pattern-recognition receptors (PRRs). These PRRs respond to a variety of exogenous pathogens or endogenous danger signals, by recognizing some highly conserved structures such as pathogen-associated molecular patterns (PAMPs) and danger/damage associated molecular patterns (DAMPs). The most studied PRRs are Toll-like receptors (TLRs). Activation of TLRs triggers production of inflammatory cytokines and type I interferons (IFNs) via myeloid differentiation primary response gene 88 (MyD88)-dependent or -independent signaling respectively, thereby modulating innate and adaptive immunity, as well as inflammatory responses. This review introduces the classification, structure, and s... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4243153 Mon, 06 Dec 2010 18:50:05 +0100 4243153 http://www.springerlink.com/content/l351464r7084jw32/ In conclusion, the peak of recurrence was almost totally concentrated in the first year; meanwhile, the first year follow-up is of critical importance in China. It may not be necessary to do the follow-up examination during the second and third years, but after three years, another colonoscopy should be undertaken. Content Type Journal ArticleDOI 10.1007/s11684-010-0200-9Authors Qin-Yan Gao, Department of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, 200001 ChinaHui-Min Chen, Department of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, 200001 ChinaJian-Qiu Sheng, Department of Gastroenterology, PLA. The Mi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4229502 Thu, 02 Dec 2010 18:17:02 +0100 4229502 http://www.springerlink.com/content/7r21163727506443/ This article reviews some of the most common mutations in CN-AML and gives a perspective of the translation of these discoveries from bench to bedside. Content Type Journal ArticleDOI 10.1007/s11684-010-0220-5Authors Felicitas Thol, Department of Hematology, Hemostasis, Oncology, and Stem Cell Transplantation, Hannover Medical School, Hannover, 30625 GermanyArnold Ganser, Department of Hematology, Hemostasis, Oncology, and Stem Cell Transplantation, Hannover Medical School, Hannover, 30625 Germany Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4229503 Wed, 01 Dec 2010 20:20:20 +0100 4229503 http://www.springerlink.com/content/681785781621w724/ Abstract  In documenting clinical experience in the diagnosis and treatment of graft versus host disease (GVHD), we retrospectively analyzed data of one case that has developed GVHD after liver transplantation. This patient exhibited fever, skin rash, and diarrhea on day 9 after liver transplantation. His liver function was normal. Skin biopsy showed scattered keratinocytes accompanied by satellite-like lymphocyte infiltration and basal cell liquefaction degeneration. After carefully analyzing the complications, we took the strategy of decreasing the dose of tacrolimus. Thereafter, the patient’s temperature decreased to normal, his skin rashes subsided, and his diarrhea was relieved. This case suggests that reducing the dosage of immunosuppressive agents can be an effectiv... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4229505 Wed, 01 Dec 2010 20:20:19 +0100 4229505 http://www.springerlink.com/content/nx32215n5384564g/ Abstract  Cystic echinococcosis (CE) (hydatidosis, hydatid disease) is a zoonosis caused by the larval stage of Echinococcus granulosus, typically affecting the liver. Hepatic cystic echinococcosis (HCE) is often asymptomatic, and symptoms occur largely when complications develop. Up to one-third of HCE can be shown their complications such as rupture of the cyst, secondary infection, and anaphylactic reaction. Clinically, patients present with pain, obstructive jaundice, cholangitis, anaphylactic reaction, and shock. Early diagnosis and treatment of complications of CE must be very important, since mortality is high when obstruction of the biliary ducts occurs, leading to ascending cholangitis and septicemia, anaphylactic shock, or even life-threatening conditions. Con... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4229504 Wed, 01 Dec 2010 20:20:19 +0100 4229504 http://www.springerlink.com/content/u34q884441382511/ Abstract  Hilar cholangiocarcinoma, first described by Klatskin in 1965, is a relatively rare tumor arising from the bile ducts. The histomorphological features of hilar cholangiocarcinoma are identical with other extra- and intra-hepatic bile duct carcinomas. The most common disease associated with cholangiocarcinoma is primary sclerosing cholangitis. The development of cholangiocarcinoma is a multistep process associated with several mutations in oncogenes and tumor-suppressor genes. Based on macroscopic appearance, three distinct subtypes have been described: sclerosing, nodular, and papillary. Microscopically, more than 95% of tumors are adenocarcinomas. Hilar cholangiocarcinoma is a slowly growing tumor and tends to spread longitudinally along the bile ducts with neura... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4209633 Fri, 26 Nov 2010 07:07:39 +0100 4209633 http://www.springerlink.com/content/um6643p41tk81241/ Abstract  In order to compare the effects of Bassini and tension-free mesh hernioplasty, a total of 552 patients with inguinal hernia who were subjected to surgical treatment in our hospital were randomly divided into the following two groups: the Bassini group (n = 269) and the tension-free mesh group (n = 283). The recurrence rates, pain, discomfort, and other complications were recorded, and the causes of the complications were explored. The recurrence rate in the Bassini group was 8.9% (24/269), significantly higher than that in the tension-free repair group (2.8%, 8/283). In addition, the recurrence rates in the Bassini and tension-free groups before 2004 were 12.6% and 5.6%, respectively, which were markedly higher than the rates after 2004 (5.3% and 0.7%, respectively... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4209632 Fri, 26 Nov 2010 07:07:39 +0100 4209632 http://www.springerlink.com/content/kh44379740135272/ Abstract  To investigate whether alcohol and tea consumption has an etiological association with nasopharyngeal carcinoma (NPC) in a high-incident population, a large scale case-control study was conducted. The study included 2846 individuals in Guangdong Province, China, with 1387 newly diagnosed cases of NPC and 1459 frequency-matched controls. Exposure histories of alcohol and tea consumption were obtained via personal interviews. Information regarding socio-demographic characteristics (age, sex, education, dialect and household type), family history of NPC, Epstein-Barr virus (EBV) infection, dietary habits and other potential confounding factors was also studied. An analysis was performed using unconditional logistic regression to calculate odds ratios (OR) and 95% con... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4209634 Fri, 26 Nov 2010 07:07:38 +0100 4209634 http://www.springerlink.com/content/3882gq66151434xj/ Abstract  Developing esophageal cancer is a multi-step process that begins with the accumulation of genetic and epigenetic alterations, and leads to the activation of oncogenes and the inactivation or loss of tumor suppressor genes (TSG). In addition to genetic alteration, epigenetic modifications, and in particular DNA methylation, are recognized as a common molecular alteration in human tumors. In esophageal cancer, aberrant methylation of promoter regions occurs not only in advanced cancer, but also in premalignant lesions. DNA methylation is related to survival time and sensitivity of chemoradiotherapy. This review is mainly focused on epigenetic changes in esophageal cancer and the value of early detection for patient prognosis, treatment choices, and potential targeti... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4206588 Wed, 24 Nov 2010 20:23:21 +0100 4206588 http://www.springerlink.com/content/t13r11t8381k0833/ Content Type Journal ArticleDOI 10.1007/s11684-010-0320-2Authors Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Sun Yat-sen University, Guangzhou, 510060 ChinaXiao-Shi Zhang, State Key Laboratory of Oncology in South China, Sun Yat-sen University, Guangzhou, 510060 ChinaQiang Liu, State Key Laboratory of Oncology in South China, Sun Yat-sen University, Guangzhou, 510060 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4204398 Tue, 23 Nov 2010 18:16:59 +0100 4204398 http://www.springerlink.com/content/j05w61g837523533/ Abstract  β-catenin is a key molecule involved in both cell-cell adhesion and Wnt signaling pathway. In our study, we found that, in the development of hepatocellular carcinoma (HCC), β-catenin was correlated with hepatitis B virus (HBV) X gene encoded protein, which is essential for HBV infectivity and is a potential cofactor in viral carcinogenesis. The expression levels of wild-type β-catenin and E-cadherin were decreased in HepG2 cells expressing hepatitis B virus X protein (HBx), accompanied by destabilization of adherens junction. Reverse transcriptase PCR (RT-PCR), Northern and Western blot showed that reduction of wild-type β-catenin expression involved degradation of the protein. However, RNA interference (RNAi) and luciferase assay indicated that HBx enhanced ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4204399 Tue, 23 Nov 2010 18:16:58 +0100 4204399 http://www.springerlink.com/content/j845720wn6333275/ Abstract  Chemical biology, using small molecules as probes to study the cellular signaling network, has developed rapidly in recent years. The interaction between chemistry and biology not only provides new insight into the understanding of cellular activities, but also generates new lead compounds for the treatment of diseases. Transcription factors and kinases such as retinoic acid receptor-alpha (RARα), acute myeloid leukemia 1 (AML1), CAAT/enhancer-binding protein α (C/EBPα), c-myc, and c-abl play important roles in the differentiation of hematopoietic stem/progenitor cells. Abnormalities in these proteins may cause the dysregulation of hematopoiesis and even the occurrence of leukemia. Ubiquitin-mediated protein degradation represents a critical mechanism in regula... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4193089 Sat, 20 Nov 2010 17:52:48 +0100 4193089 http://www.springerlink.com/content/r3t6644055885661/ Abstract  The association of viral mutations and haplotypic carriages with mutations in the preS region of hepatitis B virus (HBV) genotypes B and C with hepatocellular carcinoma (HCC) is of great significance for the prediction of this malignancy, but it remains obscure. We analyzed the preS sequences of HBV genotypes B and C from 1172 HBV-infected subjects including 231 patients with HCC. As compared with the HBV-infected subjects without HCC, C2875T, G2946C, A3054C, C3060A, T3066C, C3116T, A3120C, G3191A, A1C, C7A, C10A, A31C, C76T, G105C, and G147C in both genotypes were significantly associated with increased risks of HCC. C2875A, G2950A, G2951A, A3054T, C3060T, T3066A, T3069G, A3120T, and G3191C were significantly associated with increased risks of HCC in genotype C, ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4193090 Sat, 20 Nov 2010 17:52:47 +0100 4193090 http://www.springerlink.com/content/c12820056l4r1854/ Content Type Journal ArticleDOI 10.1007/s11684-010-0290-4Authors Zongyu John Chen, Minnesota Gastroenterology, PA, Minneapolis, MN 55414, USA Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4193091 Fri, 19 Nov 2010 17:05:56 +0100 4193091 http://www.springerlink.com/content/p08833k627m63jv0/ Abstract  To develop a standardized and well-rounded material available for hepatology research, the National Liver Tissue Bank (NLTB) Project began in 2008 in China to make well-characterized and optimally preserved liver tumor tissue and clinical database. From Dec 2008 to Jun 2010, over 3000 individuals have been enrolled as liver tumor donors to the NLTB, including 2317 cases of newly diagnosed hepatocellular carcinoma (HCC) and about 1000 cases of diagnosed benign or malignant liver tumors. The clinical database and sample store can be managed easily and correctly with the data management platform used. We believe that the high-quality samples with detailed information database will become the cornerstone of hepatology research especially in studies exploring the diagn... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4193093 Fri, 19 Nov 2010 07:38:27 +0100 4193093 http://www.springerlink.com/content/07r72203174513jp/ In conclusion, BAL is, in principle, an important therapeutic strategy for patients with acute liver failure, and may also be a bridge to liver transplantation. It requires further research and development, however, before it can enter clinical practice. Content Type Journal ArticleDOI 10.1007/s11684-010-0110-xAuthors Yi-Tao Ding, Department of Hepatobiliary Surgery, the Affiliated Drum Tower Hospital of the Nanjing University Medical School, Nanjing, 210008 ChinaXiao-Lei Shi, Department of Hepatobiliary Surgery, the Affiliated Drum Tower Hospital of the Nanjing University Medical School, Nanjing, 210008 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4193092 Fri, 19 Nov 2010 07:38:27 +0100 4193092 http://www.springerlink.com/content/m367765428701g8g/ This study was performed to determine the prognostic role of preoperative serum carbohydrate antigen (CA) 19-9 levels in the survival of patients with cholangiocarcinoma. Articles published up to June 1st, 2010 that evaluated preoperative CA19-9 levels and the prognosis of cholangiocarcinoma were collected for metaanalysis. The required information for calculating individual relative risk (RR) was extracted from the studies, and a combined overall RR was estimated. Nine eligible studies were included. One study dealt with extra-hepatic cholangiocarcinoma, while the other eight studies analyzed intra-hepatic cholangiocarcinoma. The mean methodological quality score was 74.1%, ranging from 65.5% to 82.5%. The overall RR for the nine studies was 1.28 (95% confidence interval = 1.10–1.4... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4184726 Wed, 17 Nov 2010 07:04:44 +0100 4184726 http://www.springerlink.com/content/c503454722q44184/ This study aimed to investigate the effect of adjuvant therapy on the treatment of stage II pancreatic carcinomas. The clinical data of 139 cases of stage II pancreatic carcinoma were analyzed retrospectively. The overall 1-, 3-, and 5-year cumulative survival rates of 139 patients were 40%, 6%, and 3%, respectively, and the median survival time (MST) was 279 days. The MST was 399 days for those with adjuvant therapy, 210 days for those without adjuvant therapy, 390 days for the radical resection group, 270 days for the bypass operation and laparotomy group, and 132 days for the nonsurgical group. The adjuvant therapy could not prolong the survival time and decrease the liver metastasis rate of the patients with stage II carcinoma significantly in radical resection group (P>0.05).... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=4180515 Tue, 16 Nov 2010 19:31:11 +0100 4180515 http://www.springerlink.com/content/e7q13285215k1124/ This article reviews advanced multi-center, hospital/population-based studies of most recent years, and aims to draw a definitive conclusion on HPV prevalence and genotyping in cervical cancer and precancerous lesions in China from the aspect of study population, geographic areas and time period. Content Type Journal ArticleDOI 10.1007/s11684-010-0095-5Authors Shao-Ming Wang, Department of Cancer Epidemiology, Cancer Institute of Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100021 ChinaJing Li, Department of Cancer Epidemiology, Cancer Institute of Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100021 ChinaYou-Lin Qiao, Department of Cancer Epidemiology, Cancer Institute of Chinese Academy of Medical Sciences, Peking Unio... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3948555 Wed, 08 Sep 2010 16:06:07 +0100 3948555 http://www.springerlink.com/content/v4493u0j78268668/ Abstract  Nowadays, pyogenic liver abscess (PLA) is still a common and severe intra-abdominal infection, and Klebsiella pneumoniae had emerged as the most common pathogenic bacteria worldwide in the past ten years. Our study aims to achieve an early pathogenic diagnosis and rational therapy modality for Klebsiella pneumoniae liver abscess (KLA) through clinical data analysis. A total of 197 inpatients in Zhongshan Hospital, Shanghai, diagnosed as having liver abscess between March 2001 and September 2009 were enrolled. Patients with monomicrobial infection were divided into two groups: patients with K. pneumoniae liver abscess (KLA group, n = 106) and those with non-Klebsiella pneumoniae liver abscess (NKLA group, n = 56). A retrospective analysis was made between these two gr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3883234 Wed, 18 Aug 2010 06:49:28 +0100 3883234 http://www.springerlink.com/content/jv675777g1766540/ Abstract  Family planning is a basic state policy in China. Its aim is to control population growth and to enhance population quality. Technical services are the key measures for implementing the family planning policy. In order to ensure that people use safe, effective, and appropriate contraceptive methods based on the government’s commitment, China has established countrywide family planning service networks down to the township level. The people can access various and convenient contraceptive services. In urban areas, all contraceptive services are free. The contraceptive prevalence rate in 2007 was 84.6%, the percentage of intrauterine device (IUD) was 52.3%, that of female sterilization was 32.3%, and that of vasectomy was 6.1%. This means that more than 90% of marr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866129 Fri, 13 Aug 2010 07:03:02 +0100 3866129 http://www.springerlink.com/content/a2156mh14611l56h/ Abstract  Polycystic ovary syndrome (PCOS) is a common gynecologic endocrinopathy. The pathogenesis of PCOS is associated with both heredity and environment. PCOS has adverse impacts on female endocrine, reproduction, and metabolism. PCOS can impact women’s reproductive health, leading to anovulatory infertility and higher rate of early pregnancy loss. PCOS has additional metabolic derangements, such as insulin resistance, impaired glucose tolerance, and dyslipidemia. The risks of diabetes, cardiovascular disease, hypertension, metabolic syndrome, and endometrial cancer among PCOS patients are significantly increased as well. Content Type Journal ArticleDOI 10.1007/s11684-010-0098-2Authors Zi-Jiang Chen, Center for Reproductive Medicine, Provincial Hospital Affiliate... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866130 Fri, 13 Aug 2010 07:03:01 +0100 3866130 http://www.springerlink.com/content/a43771w4l36k3736/ Abstract  Patients infected through commercial blood-selling practices (former plasma donors, FPDs) in China represent a unique patient population compared to individuals infected through sexual contact or intravenous drug use. FPDs all have an approximate time of human immunodeficiency virus (HIV) infection during the mid-1990s. Few studies about the natural history of HIV infection in these patients were performed. The current study focuses on characterizing the duration of asymptomatic infection as well as the time to disease and mortality of infected FPDs in China. A retrospective cohort study was conducted based on data collected from 5484 HIV-1 infected FPDs in Shangcai county, Henan province. Kaplan-Meier analysis was applied to estimate the asymptomatic duration fro... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866131 Thu, 12 Aug 2010 18:46:40 +0100 3866131 http://www.springerlink.com/content/cj56578002g4877l/ Abstract  Prenatal screening and diagnosis are major methods for control of birth defects, which is a very important problem in China. Here, we review current situation and development of prenatal screening and diagnosis in mainland China, including prenatal screening and prenatal diagnosis of fetal chromosome abnormalities, non-invasive prenatal diagnostic techniques and prenatal diagnosis of monogenic diseases, polygenic disease and congenital metabolic diseases. We also discuss epidemiology of birth defects and genetic diseases in China and related ethical issues of prenatal diagnosis. Content Type Journal ArticleDOI 10.1007/s11684-010-0100-zAuthors Xu-Ming Bian, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, 100730 ChinaQi ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866132 Wed, 11 Aug 2010 15:08:21 +0100 3866132 http://www.springerlink.com/content/jt3344177755t212/ Abstract  High-intensity focused ultrasound (HIFU) is a technique to destroy tissue at depth within the body, selectively and without harming overlying and adjacent structures within the path of the beam because the ultrasonic intensity at the beam focus is much higher than that outside of the focus. Diagnostic ultrasound is the first imaging modality used for guiding HIFU ablation. In 1997, a patient with osteosarcoma was first successfully treated with ultrasound imaging-guided HIFU in Chongqing, China. Over the last decade, thousands of patients with uterine fibroids, liver cancer, breast cancer, pancreatic cancer, bone tumors, and renal cancer have been treated with ultrasound imaging-guided HIFU. Based on several research groups’ reports, as well as our ten-year clin... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866135 Wed, 11 Aug 2010 15:08:20 +0100 3866135 http://www.springerlink.com/content/t866542n93605548/ In conclusion, hepatobiliary scintigraphy, which is a noninvasive, safe, valuable examination method, in combination with examination of duodenal fluid, is of value for the differential diagnosis between infantile hepatitis syndrome and biliary atresia. Content Type Journal ArticleDOI 10.1007/s11684-010-0099-1Authors Sheng-Xuan Liu, Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030 ChinaZhi-Hua Huang, Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866134 Wed, 11 Aug 2010 15:08:20 +0100 3866134 http://www.springerlink.com/content/t411265m5xx74713/ Abstract  The possibility of the 2 h oral glucose tolerance test (OGTT) as an alternative to the 3 h OGTT was investigated based on data from a national survey on pregnancy-associated diabetes. Data were retrieved from 4179 pregnant women who had OGTT performed after an abnormal 50 g glucose challenge test (GCT). All of the 4 glucose levels during their OGTT were collected and analyzed. According to American Diabetes Association (ADA) gestational diabetes mellitus (GDM) diagnostic criteria, among the 4179 pregnant women who required OGTT, 3429 (82.1%) were normal and 750 (17.9%) were diagnosed as GDM. If the 3rd h glucose levels were omitted from OGTT, 79 cases of GDM (10.5%) would be overlooked. No trend was shown where women with more risk factors were more likely to be o... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866133 Wed, 11 Aug 2010 15:08:20 +0100 3866133 http://www.springerlink.com/content/h55x447281187588/ Abstract  Recurrent spontaneous abortion (RSA), defined as three or more consecutive pregnancy losses before 20 weeks of gestation, is difficult to treat in the clinical setting. It affects 1%–5% of women of reproductive age. In the investigations of immunopathogenesis, diagnosis, and treatment of RSA since the late 1980s, it was found that RSA was associated with abnormal maternal local or systemic immune response. The pathogenesis of autoimmune RSA was mainly associated with antiphospholipid antibody (APA), while that of alloimmune RSA was due to the disturbance of maternofetal immunological tolerance. Systemic etiological screening process and diagnosis systems of RSA with immune type were developed, and anticardiolipin (ACL or ACA) + anti-β2-GP1 antibody combining mu... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3866136 Wed, 11 Aug 2010 15:08:18 +0100 3866136 http://www.springerlink.com/content/gwr002765q220716/ Abstract  Resistin is an adipokine highly related to insulin resistance (IR). The purpose of our research was to investigate how resistin influences skeletal glucose metabolism and explore its mechanisms. We constructed the recombinant plasmid pcDNA3.1 expressing resistin and then transfected it into C2C12 myocytes. The expression of resistin in C2C12 myocytes was detected by Western blotting. Glucose uptake was measured by 3H labeled glucose; glucose oxidation and glycogen synthesis was detected with 14C-labeled glucose. GLUT4 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR). We observed that resistin was expressed in transfected myocytes, and resistin decreased insulin induced glucose uptake rate by 28%–31% and inhibited the expression of GLU... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3832568 Fri, 06 Aug 2010 07:14:09 +0100 3832568 http://www.springerlink.com/content/q264676327235232/ Abstract  The autoimmune regulator (AIRE) is a crucial factor for the induction of central tolerance, and mutations in this gene lead to abnormal immune responses. However, the role of AIRE in autophagy in immune cells, especially in monocytes, is obscure. In the present study, we found that overexpression of AIRE in THP-1 human monocytes resulted in increased endogenous light chain 3 (LC3)-II level and elevated LC3 positive vesicles. Moreover, an autophagy inhibitor or knockdown of AIRE by small interference RNA attenuated these effects. In contrast, the expression of p62/SQSTM1 remained unchanged in THP-1 cells after the corresponding treatment. Our findings indicate that AIRE plays a role in the regulation of autophagy in THP-1 human monocytes. Content Type Journal A... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3832567 Fri, 06 Aug 2010 07:14:09 +0100 3832567 http://www.springerlink.com/content/008110p226705298/ In conclusion, HT is still the most effective method of relieving climacteric symptoms for many postmenopausal women. However, a possible risk of breast cancer associated with long-term HT usage should not be ignored. With respect to prevention of breast cancer, regular evaluation of individual breast cancer susceptibility and close follow-up through mammography and/or breast sonography are necessary strategies for the safety of HT use. Content Type Journal ArticleDOI 10.1007/s11684-010-0093-7Authors Ling Xu, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100730 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3832569 Fri, 06 Aug 2010 07:14:08 +0100 3832569 http://www.springerlink.com/content/k021232p51u4348u/ Abstract  Cervical lesions have been regarded as the common and frequently occurring diseases in China. Recently, the morbidity and youth tendency of cervical cancer have gradually increased. Cervical cancer, related with human papilloma virus (HPV) infection, has been one of the severest diseases threatening health and life of women, and is an infectious disease. The universality of HPV infection in the reproductive tract should not be ignored. The well-known risk factors of HPV infection in cervical lesions consist of high-risk sexual behaviors, immunosuppression, age, contraceptive methods, the concurrent infection of other sexually transmitted diseases, etc. The variation of cervical lesions induced by HPV infection is involved in the continuous pathological process, in... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3774254 Sat, 17 Jul 2010 06:09:58 +0100 3774254 http://www.springerlink.com/content/1v4x3587674368tj/ This study was aimed to detect maternal serum levels of thromboxane B2 (TXB2) in different durations of pregnancy and explore the predictive value of TXB2 for preeclampsia. By employing a prospective and double-blind study method, 180 pregnant women with previously normal blood pressures during their progestation were included in the study. Peripheral venous blood samples were obtained during 10+ 0–14+ 6 (period I), 20+ 0–24+ 6 (period II) and 30+ 0–34+ 6 (period III) weeks of gestation. Maternal serum levels of TXB2 were measured by enzyme-linked immunoassay. The pathogenetic condition and pregnancy outcomes of these cases were observed. (1) Among the 180 previously normotensive women, ten developed preeclampsia (case group; four severe and six mild preeclampsia). One hundred a... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3642667 Wed, 02 Jun 2010 17:07:39 +0100 3642667 http://www.springerlink.com/content/g32m278175100m02/ Content Type Journal ArticleCategory EditorialDOI 10.1007/s11684-010-0044-3Authors Linda S. Birnbaum, National Institute of Environmental Health Sciences, National Toxicology Program Research Triangle Park NC 27709 USA Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 Journal Volume Volume 4 Journal Issue Volume 4, Number 2 / June, 2010 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3642668 Wed, 02 Jun 2010 17:07:38 +0100 3642668 http://www.springerlink.com/content/5wu355375h840425/ Abstract  The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 125... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3583421 Wed, 19 May 2010 10:58:35 +0100 3583421 http://www.springerlink.com/content/0767654mp486l0r3/ This study intended to find out the relationships between the environmental changes and the incidence of hemorrhagic fever with renal syndrome (HFRS) in the Big Three Gorges Area (BTGA) of Chongqing Municipality, China. Landuse thematic maps were applied in the research. The results show that there was a significant relationship between the land-use change in the grass area and the cumulative HFRS incidence (correlation coefficient = − 0.676, P = 0.011). In the BTGA, the land was submerged because water filled in the reservoir, and meanwhile, the government called for returning land for farming to forestry. It is concluded that the changes of environment may influence the incidence of HFRS. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-010-0042-5Author... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3579279 Tue, 18 May 2010 08:17:18 +0100 3579279 http://www.springerlink.com/content/g117073k626411n8/ In conclusion, NRTIs could induce mutation of mtDNA HV2, which might contribute to the development of LD. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-010-0038-1Authors Ya-Song Wu, Chinese Center for Disease Control and Prevention Division of Treatment and Care, National Center for AIDS/STD Control and Prevention Beijing 102206 ChinaXin-Yue Chen, Capital Medical University Center for Infectious Diseases, Beijing You’an Hospital Beijing 100069 ChinaYing Shi, Capital Medical University Center for Infectious Diseases, Beijing You’an Hospital Beijing 100069 ChinaHao Wu, Capital Medical University Center for Infectious Diseases, Beijing You’an Hospital Beijing 100069 ChinaDe-Xi Chen, Capital Medical University Center for Infectious Diseases, Beijing You�... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3579278 Tue, 18 May 2010 08:17:18 +0100 3579278 http://www.springerlink.com/content/p34431423lw165l2/ Abstract  The aim of this study was to investigate epidemiological characteristics of co-infection of HIV and intestinal parasites in a HIV/AIDS highly endemic area in China. A cross-sectional survey was carried out in two villages where HIV/AIDS prevalence in residents was over 1%. Stool samples of all residents in the two targeted villages were examined for the presence of intestinal parasites. Blood examination was performed for the HIV infection detection and anemia test. A questionnaire survey was carried out in all residents participating in the study. A total of 769 individuals were enrolled in the investigation, 720 of whom were involved in stool examination of intestinal parasites. The infection rates of parasites in the residents of the targeted villages were as f... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3579280 Tue, 18 May 2010 08:17:17 +0100 3579280 http://www.springerlink.com/content/x00571q374t842m5/ Abstract  Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of Angiostrongylus cantonensis in Pomacea canaliculata. Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310 g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumu... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3579281 Tue, 18 May 2010 08:17:16 +0100 3579281 http://www.springerlink.com/content/w4753k4895065r12/ Abstract  One hundred and fifty-three isolates from the environment and 36 reference strains of the Legionella were studied with regards to their composition of cellular fatty acids as determined by gas chromatography, and then were classified into 41 groups by numerical analysis. Most reference strains formed only a single group, except L. micdadei, L. jamestowniensis, L. parisiensis, L. jordanis, L. feeleii and L. longbeachae, which were clustered into two or three groups. Even serological types of L. pneumophila could be clearly identified. Therefore, in this study, numerical analysis of cellular fatty acid composition is an effective method for identifying Legionella species. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-010-0031-8Authors Cu... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3579282 Tue, 18 May 2010 08:17:14 +0100 3579282 http://www.springerlink.com/content/6447h23434281765/ This article discusses the concept of health management and stresses that community health management should be the starting point of community health service, necessitating a paradigm shift from observing the treatment-oriented medical model to observing the prevention-and-health management-based medical model. Community health construction has entered a new phase, one which highlights the need to train general practitioners in practice-based life cycle health management. In addition, general practitioners should assume the mission of implementing community health management. Content Type Journal ArticleCategory Health Policy ForumDOI 10.1007/s11684-010-0041-6Authors Kan Zhang, Shanghai Municipal Health Bureau Department of Medical Science & Technology & Education Shanghai 2004... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3571430 Sat, 15 May 2010 08:16:23 +0100 3571430 http://www.springerlink.com/content/lmn0841374367555/ Abstract   Legionella is a causative agent of legionellosis or Legionnaires’ disease. Infection with Legionella spp. is an important cause of community and hospital-acquired pneumonia. Moreover, infection with Legionella is one of the most common causes of severe pneumonia in community settings, and Legionella is isolated in 40% of hospital-acquired pneumonia cases. The clinical features of legionellosis or Legionnaires’ disease are not unique, which makes the diagnosis challenging. Macrolides and fluoroquinolones are the most widely used drugs in treatment. Early recognition, treatment and effective measures for prevention and control of the disease are important and required. Although there have been a lot of methods for diagnosis of Legionella infection, they were ofte... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3565218 Thu, 13 May 2010 08:58:47 +0100 3565218 http://www.springerlink.com/content/v5k57362t12j8m68/ Abstract  Hand, foot and mouth disease (HFMD) is an acute viral illness that primarily affects infants and young children, and often occurs in clusters or outbreaks. The major causative agents of HFMD are coxsackievirus A16 (CVA16), human enterovirus 71 (HEV71) and coxsackievirus A10 (CVA10), of the genus Enterovirus in the family Picornaviridae. HFMD caused by EV71 is associated with severe neurological complications and death. Since the late 1990s, several major epidemics of EV71 HFMD have swept through the Asia-Pacific region, associated with a rapid fulminant course, severe neurological complications, and a large number of fatalities. Until now, little has been known about the genetics and transmission trends of the fast-mutation virus, and there is no effective and spec... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3565217 Thu, 13 May 2010 08:58:47 +0100 3565217 http://www.springerlink.com/content/b8w2j14771315463/ Abstract  Human schistosomiasis, caused mainly by three principal species including Schistosoma japonicum, S. mansoni, and S. hematobium, remains a major public health concern worldwide. S. japonicum is prevalent in southern China, being a major disease risk for 66 million people. The blood fluke has a complex life cycle for survival: as a free-living form in fresh water and as a parasite in the snail intermediate and vertebrate definitive hosts. Systems-based biomedical analyses, including genomic, transcriptomic, proteomic and metabonomic approaches, have been performed on the schistosome. These comprehensive investigations have not only characterized the genomic features but also chartered gene and protein expression profiles across genders and developmental stages. The i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3565219 Thu, 13 May 2010 08:58:44 +0100 3565219 http://www.springerlink.com/content/6t48631681163513/ Abstract  The inhibitory effect of different reperfusion periods 45 min following hepatic ischemia on the expression of cholecystokinin (CCK) and vasoactive intestinal peptide (VIP) in the jejunum and the effect of salvia miltiorrhiza pretreatment were investigated, and the possible mechanism and implications were explored. Eighty rats were randomly divided into four groups: normal control group (CO group), sham-operated group (SO group), ischemia/reperfusion (I/R) injury group (IR group) and salvia miltiorrhiza pretreatment group (SM group). The rat model of I/R was established by using a non-invasive artery clamp to clip (45 min) or relax the hepatic pedicle. In the SM group, saline (40 mL/kg) and salvia miltiorrhiza injection (6 g/kg) were injected via the tail vein 30 m... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3562041 Tue, 11 May 2010 08:27:15 +0100 3562041 http://www.springerlink.com/content/n172887l47h54047/ Abstract  Gastroesophageal reflux disease (GERD) was diagnosed and fundoplication was performed, which relieved the symptoms. This paper explores the effect of fundoplication on GERD-related respiratory symptoms. From January 2007 to October 2008, 64 patients with respiratory symptoms related to GERD were consecutively enrolled for laparoscopic fundoplication. Of these, 60 patients had respiratory symptoms mainly combined with gastroesophageal reflux, while four had respiratory symptoms only. All patients had GERD confirmed, and medical treatment provided before surgery resulted in an insufficiently favorable effect or even producing further complications. All the patients were requested to fill a questionnaire form regarding the severity of symptoms and their medication so... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3554079 Sat, 08 May 2010 10:03:02 +0100 3554079 http://www.springerlink.com/content/v5037444746jml77/ Abstract  Tumor metastasis is the leading cause of death for gastric cancer. Metastasis is the main reason for the failure of clinical treatment for gastric cancer. In order to find metastasis-related genes and abnormal signal transduction pathway of high-invasive gastric cancer, samples of gastric cancer with liver metastasis were collected for microarray detection; up-regulated or down-regulated genes in all three cases were simultaneously screened out. Subsequently, from the preliminary screened genes, molecular pathways possibly impacting liver metastasis from gastric cancer were investigated by the Gene Cluster with Literature Profiles (GenCLip) analysis software. Many biological effects including apoptosis have been validated. Functional analysis of differentially exp... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3554082 Sat, 08 May 2010 10:02:59 +0100 3554082 http://www.springerlink.com/content/m14612137251166p/ Abstract  To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included B. suis (bv. 1, 2, 3, 5) and B. ovis; cluster 2 included B. abortus (bv. 3, 4, 5, 6) and B. melitensis (bv. 1, 2, 3); cluster 3 included B. abortus (bv. 1, 2, 7, 9) and B. neotomae;... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3554081 Sat, 08 May 2010 10:02:59 +0100 3554081 http://www.springerlink.com/content/w882g44786005vp6/ Abstract  Obesity is a medical condition in which excess body fat has accumulated to an extent and may have an adverse effect on health, leading to reduced life expectancy, impaired energy homeostasis and increased health problems. The p160 steroid receptor coactivator (SRC) gene family members have been suggested to be involved in energy homeostasis, but the impact of SRC-3 ablation on white and brown adipose tissue needs to be elucidated. In the current study, we collected in vivo data and carried out morphological studies on the effect of SRC-3 deficiency on white adipose tissue (WAT) and brown adipose tissue (BAT). Primary cells were cultured to investigate the differentiation ability of both adipocytes. Western blot was applied to detect the expression of master genes g... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3554080 Sat, 08 May 2010 10:02:59 +0100 3554080 http://www.springerlink.com/content/lg5054245ph87781/ Abstract  The pandemic H1N1 2009 influenza virus has caused the first influenza pandemic of the 21st century, leading to disproportionate fatalities in the low-risk population despite the generally mild nature of the illness. Advances in science and technology have allowed very detailed study on the pathogenesis of this novel virus, and many have already been published in less than a year after the start of the pandemic. Information generated from cell lines, animal models, and clinical data analysis has provided us with greater understanding of the behavior of this virus and the associated host response. The new knowledge will allow us to formulate scientifically sound and evidence-based management plans. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-01... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3523589 Thu, 29 Apr 2010 21:37:44 +0100 3523589 http://www.springerlink.com/content/v722007uw5187243/ In conclusion, the DCIONPs we synthesized are able to carry plasmid DNA and enhance the early transfection efficiency when using a magnetic field. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-010-0032-7Authors Jian Xin, Huazhong University of Science and Technology Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaYan Luo, Wuhan No. 1 Hospital Department of Ophthalmology Wuhan 430022 ChinaZe-Feng Xia, Huazhong University of Science and Technology Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaKai-Xiong Tao, Huazhong University of Science and Technology Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaKai-Lin Cai, Huazhong U... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3523588 Thu, 29 Apr 2010 21:37:44 +0100 3523588 http://www.springerlink.com/content/thxr620410630p55/ This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P < 0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P > 0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly highe... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3513163 Tue, 27 Apr 2010 07:04:13 +0100 3513163 http://www.springerlink.com/content/w1539q6848p762kk/ This study examined the inhibitory effect of triptolid (TP) on tumor necrosis factor-α (TNF-α) secreted from peripheral blood monocular cells (PBMCs) and the association of the inhibitory effect with TNF-α-308 gene polymorphisms in rheumatoid arthritis (RA) patients. Gene polymorphism at A-G site 308 in the promoter region of TNF-α gene was detected in 42 RA patients by using allele specific polymerase chain reaction (AS-PCR) assay. PBMCs were harvested from these patients and treated first with lipopolysaccharides (LPS) and then with different doses of TP (1, 5.4 and 15 ng/mL). The TNF-α level in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA). The results showed that TNF-α level in the supernatants of TP (1 ng/mL)-treated PBMCs was decreased by 3.80... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3509520 Sat, 24 Apr 2010 08:10:00 +0100 3509520 http://www.springerlink.com/content/1r2kt1h6052wp202/ Abstract  The aim of this paper is to study the effect of bradykinin (BK) on bradykinin-B2 receptor as well as the possible involved signal transduction pathways in cultured rat aortic vascular smooth muscle cells (VSMCs). Rat aortic VSMCs were cultured. Cells after 4–6 passages were used in the experiment. VSMCs were incubated with BK, BK + B2 receptor inhibitor (HOE-140), BK + MEK inhibitor (PD98059), BK + mitogen-activated protein kinase (MAPK) inhibitor (apigenin), BK + phosphoinositide 3-kinase (PI3K) inhibitor (LY294002), and BK + Akt inhibitor to evaluate the expression of B2 receptor and phosphorylation of signaling molecules MAPK, Akt, and PI3K by Western blot. (1) BK markedly up-regulated the expression of B2 receptor in VSMC. (2) Apigenin, PD98059, Akt inhibito... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3481711 Fri, 16 Apr 2010 05:44:00 +0100 3481711 http://www.springerlink.com/content/d1071675111x5qwp/ Abstract  The surgical emergency treatments and curative effects of postrenal acute renal failure following obstruction due to upper urinary calculi in infants were evaluated. Of the 13 infants with postrenal acute renal failure following obstruction due to upper urinary calculi, 11 received retrograded catheterizations of the ureter with semi-rigid ureteroscopy (F 6.8), and two received open ureterolithotomy. The results showed that only one infant had anuresis and continuous reduction of hemoglobin 5 h after the open ureterolithotomy and received exploration via excision and peritoneal dialysis, and the remaining 12 patients well recovered in this group. The renal function of all the patients was restored without postoperative complications. It is concluded that the retro... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274098 Sun, 14 Feb 2010 06:42:04 +0100 3274098 http://www.springerlink.com/content/w08j903t6870kw43/ Content Type Journal ArticleCategory EditorialDOI 10.1007/s11684-010-0022-9Authors Sai-Juan Chen, Rui-Jin Hospital Affiliated with Shanghai Jiao Tong University School of Medicine Shanghai Insitute of Hematology Shanghai ChinaXiao-Ping Chen, Huazhong University of Science and Technology Tongji Hospital, Tongji Medical College Wuhan ChinaGuang-Biao Zhou, Chinese Academy of Sciences Institute of Zoology Beijing China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 Journal Volume Volume 4 Journal Issue Volume 4, Number 1 / March, 2010 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274097 Sun, 14 Feb 2010 06:42:04 +0100 3274097 http://www.springerlink.com/content/v311223742233774/ Abstract  China has been undertaking a profound reform on health care. Although more than 1.16 billion people have been covered by rural and urban medical insurance to date, the level of reimbursement from insurance is very limited, especially for critical diseases such as leukemia. This places heavy economic burdens on patients. Under these circumstances, systems innovation is imperative for the efficient utilization of limited funding. In this respect, certain valuable experience from other countries may prove helpful. The prospective payment system of Diagnosis-related Groups (DRGs), Clinical Paths, and the Comparative Effectiveness Analysis adopted by the National Institute of Health and Clinical Excellence (NICE, UK), can be fine tools to reduce medical costs and impro... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274099 Sun, 14 Feb 2010 06:42:03 +0100 3274099 http://www.springerlink.com/content/u836414429632241/ This study was conducted to evaluate the global and regional left ventricular twist by 2D strain in patients with anterior wall myocardial infarction (AMI). A total of 55 AMI patients were divided into two groups according to their ejection fraction (EF) values (group A: LVEF ⩾ 50%; group B: LVEF < 50%), and 35 normal people served as the control group. Using 2-dimensional strain software, global and regional LV rotation and displacement were obtained at two planes. Compared with the control group, patients of group A showed reduced peak LV twist of the anterior and anterior-septal wall (9.26±1.89 vs 10.74±2.67; 9.71±1.71 vs 11.36±2.29, both P < 0.05), but the radial displacement and global twist were maintained (P > 0.05). Differently, regional and global LV twist and ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274101 Sun, 14 Feb 2010 06:42:02 +0100 3274101 http://www.springerlink.com/content/u60k55n1q8726m6x/ Abstract  We conducted a trial to assess the influence of closure or nonclosure of the peritoneum and subcutaneous tissue on the clinical outcomes of cervical cancer patients who underwent radical hysterectomy with lower abdominal cross incision. This randomized controlled trial was performed on 158 cervical cancer patients in our hospital between January 2002 and June 2004. Eighty-two patients were allocated to the “closure” group and 76 patients to the “nonclosure” group. Results showed that non-closure of the peritoneum and subcutaneous tissue could shorten operation time and febrile duration, reduce antibiotics requirement, increase the volume of drainage and decrease the incidence of liquefaction of subcutaneous fat (P < 0.05). There was no difference in bloo... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274100 Sun, 14 Feb 2010 06:42:02 +0100 3274100 http://www.springerlink.com/content/b213820n572v9g12/ Abstract  One hundred and twenty-six patients above 80 years old with biliary diseases undergoing operations in Shaoxing People’s Hospital from Jan. 2002 to Jan. 2007 were analyzed retrospectively. All patients performed a preoperative stair-climbing test, and the risks for cardiopulmonary complications were evaluated with pairmatching and linear correlations analysis between stairclimbing height (h) and left ventricular ejective factor (EF), forced vital capacity (FVC) and forced expiratory volume in one second (FEV1). There was a significant difference in the incidence of cardiopulmonary complications among different stair-climbing heights. Stair-climbing heights were positively related with EF, FVC and FEV1. This suggests that the stair-climbing test is an effective and... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274102 Sun, 14 Feb 2010 06:41:59 +0100 3274102 http://www.springerlink.com/content/a1t3t1030wq427j8/ Abstract  Hepatitis B virus X protein (HBx), a 17-kd protein encoded by X gene of hepatitis B virus (HBV), has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of the study is to investigate the extracellular regulated protein kinases (ERKs) pathway of HBx on glomerular mesangial cell (GMC) proliferation and tumor necrosis factor-α (TNF-α) expression. The HBV X gene was amplified by polymerase chain reaction (PCR), inserted into the eukaryotic expression vector pCI-neo and confirmed by restriction endonuclease digestion and sequence analysis. PCI-neo containing HBV X gene (pCI-neo-X) was then transfected into cultured GMC line via liposome. GMC proliferation, TNF-α and its mRNA expression w... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274104 Sun, 14 Feb 2010 06:41:58 +0100 3274104 http://www.springerlink.com/content/j353171h87268736/ This study was to examine the functional significance of XPF promoter polymorphisms and their association with lung cancer risk. The function of XPF promoter polymorphisms was tested by a set of biochemical assays, and their effects on lung cancer risk were determined by a case-control analysis of 988 patients with lung cancer and 986 controls. The XPF — 673T allele showed a significantly higher transcriptional activity as compared with the −673C allele. The −673TT genotype was associated with a decreased risk of lung cancer compared with the CC genotype (adjusted OR = 0.62, 95% CI = 0.42–0.91; P = 0.015) and this effect was more significant among males (adjusted OR = 0.55, 95% CI = 0.35–0.86; P = 0.009), elder subjects (adjusted OR = 0.51, 95% CI = 0.30–0.86; P = 0.012), a... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274103 Sun, 14 Feb 2010 06:41:58 +0100 3274103 http://www.springerlink.com/content/r348553t37p01692/ This study was to investigate a better way to detect and differentiate primary, residual, recurrent nasopharyngeal carcinoma (NPC) lesions post-radiotherapy in patients with NPC by means of routine computed tomography (CT) in combination with 99Tcm-sestamibi single photon emission computed tomography (99Tcm-MIBI SPECT). Forty-eight patients with histologically confirmed primary NPC underwent 99Tcm-MIBI SPECT at the 3rd month before and after radiotherapy, and at the 6th month after radiotherapy. All patients had contemporaneous CT examinations. Histopathologic results and/or clinical follow-up data (over 18 months) were used as the golden standard for evaluating residual/recurrent lesions. The radioactive count ratio of nasopharynx to scalp was obtained as the MIBI uptake index (MUI). ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274105 Sun, 14 Feb 2010 06:41:57 +0100 3274105 http://www.springerlink.com/content/862116745k812219/ This study investigated the molecular types of the Cryptococcus neoformans species complex isolates and their clinical manifestations among human immunodeficiency virus (HIV)-positive and HIV-negative patients in southeast China in the past 15 years. The molecular types of 109 isolates from 108 patients were analyzed by the PCR fingerprinting method, sequences of internal transcribed spacers of rDNA (ITS region), and sequences of the capsule-associated gene (CAP59). In HIV-positive patients, clinical isolates were grouped into molecular types VNI (75%, 15/20), VNII (15%, 3/20), and VNIII (10%, 2/20). In HIV-negative patients, the majority of the clinical isolates were grouped into molecular types VNI (72%, 64/89), VNII (13%, 12/89), VGI (12%, 11/89), VNIII (1%, 1/89), and VGII (1%, 1/... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274108 Sun, 14 Feb 2010 06:41:56 +0100 3274108 http://www.springerlink.com/content/g33012j7782388p0/ Abstract  Stroke is a major cause of adult death and disability worldwide. Epidemiological and animal studies have provided strong evidence that the pathogenesis of stroke is multi-factorial and induced by a combination of environmental and genetic risk factors, but the identification of individual causative variants remains little known. Genetic influences are likely to be polygenic with small effect sizes, and stroke itself consists of a number of different subtypes which may each have different genetic profiles. In addition, various ethnic populations may have different stroke risk, such as Asian race. The reasons for high risk of stroke among the Chinese, especially hemorrhagic stroke, remain unknown. Most human studies have taken a candidate gene approach using case-co... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274107 Sun, 14 Feb 2010 06:41:56 +0100 3274107 http://www.springerlink.com/content/qg037p3248521251/ Abstract  Cardiovascular diseases (CVD) cause one third of all deaths in China, and the number is anticipated to double by 2020. They also greatly result in disability and adjusted life year loss. The prevalence of CVD has become a new burden for China, due to an aging population, smoking, and changes in dietary habits and lifestyles. As the largest developing country, China needs to tailor her own national policies for managing CVD with full consideration of epidemiology, local needs, and affordability. Smoking cessation, increased physical activity, control of hypertension and hypercholesterolemia, and maintenance of a “traditional Chinese” diet should be important strategies for reducing the burden of CVD in China. Health officials in China should take their responsi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274106 Sun, 14 Feb 2010 06:41:56 +0100 3274106 http://www.springerlink.com/content/y4t1473p1622u822/ Content Type Journal ArticleCategory News & ViewsDOI 10.1007/s11684-010-0024-7 Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 Journal Volume Volume 4 Journal Issue Volume 4, Number 1 / March, 2010 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3274109 Sun, 14 Feb 2010 06:41:54 +0100 3274109 http://www.springerlink.com/content/x728168542415260/ Abstract  Mitogen-activated protein kinase (MAPK) signaling pathway, one of the most important signaling pathways in eukaryotic organism, is involved in multiple cellular events such as cell growth, differentiation, and apoptosis. MAPK is of great importance to the normal function of organisms, while its dysfunction results in various diseases. So far, inhibitors specifically against each subfamilies of MAP kinase have been developed, while more endeavors are needed to discover the compounds selectively targeting a particular subfamily member. Most of the kinase inhibitors exert their functions in an ATP-competitive way or a non-ATP-competitive way. Further studies on the effective mechanism of the MAPK inhibitors and their therapeutic roles in the treatment of diseases are... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241573 Wed, 03 Feb 2010 06:56:08 +0100 3241573 http://www.springerlink.com/content/n43n332v1m8r7752/ Abstract  Venous thromboembolism (VTE), including deep vein thrombosis and pulmonary embolism, carries significant mortality and morbidity. The most important and effective way to reduce VTE incidence is to identify the patients at risk and give necessary prevention. VTE is a multifactorial and complicated disorder. Major risk factors for VTE include surgery and trauma, acute medical illness, active cancer and pregnancy. Genetic factors increase susceptibility to the disease and are useful in predicting the development of VTE. Gene-gene and gene-environment interactions alter and magnify the clinical picture in this disorder. This brief review summarizes some selected clinical and genetic risk factors for VTE based on the current research in China. Content Type Journal Ar... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241574 Wed, 03 Feb 2010 06:56:07 +0100 3241574 http://www.springerlink.com/content/d4w02m174712q633/ This study aims to investigate the genetic association between the polymorphism of cytosolic phospholipase A2 (cPLA2) family genes and schizophrenia among Han Chinese in the northern part of China. The polymerase chain reaction-based ligase detection reaction (PCR-LDR) was applied to detect the genotype ten single nucleotide polymorphisms (SNPs) of cPLA2 family genes among 201 pedigrees consisting of fathers, mothers and affected offsprings with schizophrenia. The pedigrees were collected from 2000 to 2006. Haplotype relative risk (HRR) test, transmission disequilibrium test (TDT), haplotype transmission analysis and multiple locus analysis were conducted to analyze the genotyping data. The genotypic frequency of cPLA2 gene did not deviate from Hardy-Weinberg equilibrium either in ca... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241576 Wed, 03 Feb 2010 06:56:06 +0100 3241576 http://www.springerlink.com/content/wtv1u0417tr50817/ Abstract  Normal brain function requires continuous supply of oxygen and glucose in a strict manner. The close spatial and temporal relationship between neural activity and cerebral blood flow (CBF) is termed as neurovascular coupling, which is dependent or not dependent on astrocytes. The increase in flow evoked by brain activity is mediated by the concerted action of multiple mediators that originate from different cells and act at different levels of the cerebral vasculature. More studies are needed for further understanding the precise mechanisms underlying neurovascular coupling, especially the implications of astrocytes. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-010-0019-4Authors You Zhou, Dalian Medical University Institute for Brain Disorders ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241575 Wed, 03 Feb 2010 06:56:06 +0100 3241575 http://www.springerlink.com/content/v675075474m29385/ This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all P ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241577 Wed, 03 Feb 2010 06:56:05 +0100 3241577 http://www.springerlink.com/content/lv75603282668807/ This study aimed to identify functional single nucleotide polymorphisms in the cyclooxygenase-2 gene promoter and evaluate their effects on the risk of primary hepatocellular carcinoma (HCC) with hepatitis B virus (HBV) infection. We conducted a population-based, case-control study enrolling 630 Han Chinese people in Hubei province. Subjects included primary HCC patients with HBV infection (n = 210), chronic hepatitis B cases (n = 210) and healthy Han Chinese (n = 210). −1195G/A polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing analysis. We found -1195A allele carriers had a higher risk of HCC with HBVinfection (OR, 0.72; 95% CI, 0.548–0.946). The −1195A allele might be used as a marker in screening indiv... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241578 Wed, 03 Feb 2010 06:56:04 +0100 3241578 http://www.springerlink.com/content/4k701w5514gn3837/ Abstract  A community-based multi-center randomized controlled trial was conducted to evaluate the effectiveness of blood pressure control for hypertension patients in communities in urban Shanghai by integrated intervention. At present, patients (n = 1395) from four communities have completed follow-up for one year, including the intervention group (n = 921) and usual care group (n = 474). The intervention programs included disease management by a care manager. Blood pressure of each patient was measured regularly. Compared with the control group, the net change of mean systolic blood pressure (SBP) was − 6.75 (95% CI: − 7.79 to − 5.71, P < 0.001) mmHg, mean diastolic blood pressure (DBP) was − 4.29 (95% CI: − 5.08 to − 3.49, P < 0.001) mmHg, and mean pulse ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241579 Wed, 03 Feb 2010 06:56:03 +0100 3241579 http://www.springerlink.com/content/b1283k8470p72283/ Abstract  Severe sepsis and septic shock affect millions of patients and are major causes of mortality worldwide. Advancements in treatment and disease management led to a decline in in-hospital mortality from 27.8% (1979–1984) to 17.9% (1995 to 2000). In this article, we systemically review recent guidelines for the management of severe sepsis and septic shock published in 2008 by the International Surviving Sepsis Campaign Guidelines Committee. The 2008 Surviving Sepsis guidelines recommend protocolized resuscitation with goals to maintain central venous pressure ⩾ 8–12 mmHg, mean arterial pressure ⩾ 65 mmHg, urine output ⩾ 0.5 mL·kg−1·h−1 and central venous oxygen saturation ⩾ 70% (or mixed venous ⩾ 65%). Further fluid administration, transfusion of pac... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3241580 Wed, 03 Feb 2010 06:56:02 +0100 3241580 http://www.springerlink.com/content/137463jv2r121u13/ Abstract  Vulnerable atherosclerotic plaque rupture leading to thrombosis is the major cause of acute coronary syndrome (ACS). Studies on the pathophysiologic mechanism of both ACS and plaque stabilizing treatment are driving the development of animal models of vulnerable plaque. In our laboratory, we established animal models of plaque rupture and thrombosis in rabbits and mice that are similar to human plaque rupture. Potential mechanisms involved in plaque vulnerability were studied from the inflammation-immunity, proliferation-apoptosis, oxidative stress and biomechanics aspects. Imaging markers and biomarkers were used to detect vulnerable plaques, including high frequency duplex ultrasound, intravascular ultrasound (IVUS), intravascular ultrasound elastography, magnet... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=3224111 Thu, 28 Jan 2010 18:05:05 +0100 3224111 http://www.springerlink.com/content/f0p5540m73864335/ Abstract  The authors performed a meta-analysis of case-control studies that addressed whether reproductive factors and oral contraceptive use were associated with breast cancer by searching the MEDLINE, PubMed, Proquest, Embase, ScienceDirect, African Healthline, BMJ Health Intelligence and Chinese Periodical net databases for all English-language and Chinese-language papers published from January 1, 1997 to December 31, 2007. A total of 15 studies calculating pool ORs indicated that menopausal age >50 yr [odds ratio (OR), 1.39; 95% confidence interval (CI), 1.22–1.57] and oral contraceptive use (OR, 2.12*, “*”: summary OR was adjusted; 95% CI, 1.24–3.62) were correlated with the increase in breast cancer risk while the summary OR based on number of full-term pr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2986913 Tue, 10 Nov 2009 18:25:04 +0100 2986913 http://www.springerlink.com/content/yt8414142245t5n4/ Abstract  Few studies have been conducted to assess the level of physical activity (PA) of women in China. The purpose of this study was to describe the prevalence of PA among women in Wuhan and explore sociodemographic and behavioral correlation to PA for the population. A total of 1359 community-dwelling women (42.97±13.60 years old) participated in this study. The long-version IPAQ was used to define the level of PA. 45.7% of women were considered physically active, and 27.5% were vigorously active. The proportion of physical inactivity was around 26.8%. The total and 4 domains of PA showed significant age, education level, and occupation variation. PA tended to be lower as the age declined. PA in transportation, housework, leisure time, and the total tended to be the l... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2986912 Tue, 10 Nov 2009 18:25:04 +0100 2986912 http://www.springerlink.com/content/44u3768311415714/ Abstract  A murine primary mammary tumor model was established to investigate the treatment with ginsenosides Rg3. The relationship between ginsenosides Rg3 and primary mammary tumor was explored. Mammary tumor was induced by using the 7,12-dimethybenz(a)anthracene (DMBA). Ginsenoside Rg3 was employed for treatment. The incidence of mammary tumor in every group was compared, and the expressions of vascular endothelial growth factor (VEGF) and microvessel density (MVD) were detected by immunohistochemical method. The cell cycle and apoptosis percentage were determined by means of flow cytometry. The incidence of tumor in treatment group was significantly lower than that in control group (60.00% vs 33.33%, P < 0.05). The average diameter of mammary tumor was (0.86±0.27) cm... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979239 Sat, 07 Nov 2009 19:05:56 +0100 2979239 http://www.springerlink.com/content/b24401666211m522/ This study is mainly aimed at evaluating the expression of PC-cell-derived growth factor (PCDGF) in breast cancer and breast adenofibroma, and to compare with other commonly used clinical pathological indices, then to investigate the diagnostic and targeted therapeutic purpose of PCDGF in breast cancer tissue. In this study, we detected the expression of PCDGF, p53 and CerbB-2 in breast cancer tissue and the expression of PCDGF in breast adenofibroma tissue by immunohistochemical method, and analyzed the relationship between them. We found that PCDGF was expressed in most breast cancer tissue, but was not in breast adenofibroma tissue, and the expression of PCDGF was related with the tumor’s pathological category and the expression of estrogen receptor (ER) and progesterone recepto... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979241 Sat, 07 Nov 2009 19:05:50 +0100 2979241 http://www.springerlink.com/content/bn8678u237207226/ In conclusion, the EBV LMP2 DNA vaccine can induce a strong humoral immune response in mice. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0087-5Authors Jianqing Pan, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaQin Zhang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaDaowen Wang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2979240 Sat, 07 Nov 2009 19:05:50 +0100 2979240 http://www.springerlink.com/content/y553174670p36381/ Abstract  The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated. Rat models of myocardial hypertrophy were established by using abdominal aorta partial coarctation method. Forty-eight rats were randomly divided into sham group (S group), model group (M group), valsartan treatment group (X group), low-dose tanshinone treatment group (LD group), medium-dose tanshinone treatment group (MD group), and high-dose tanshinone treatment group (HD group) (n = 8 in each group). Left ventricular mass index (LVMI), left ventricular posterior wall (LVPW), and septal thickness (IVS) were detected by high frequency ultrasonography. Myocardial fiber diameter (... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971554 Fri, 06 Nov 2009 19:47:49 +0100 2971554 http://www.springerlink.com/content/p75050n5357801gh/ Abstract  Thl7 cells, a special kind of auxiliary type T cells, can secrete IL-17A, IL-17F, IL-21, IL-22, etc., as a newly discovered T-cell subset in recent years. As a different subset from the Thl and Th2 cells, Th17 cells play an important role in the development of a variety of autoimmune diseases. A current study shows that the IL-6 inflammatory response of the organization in combination with the occurrence of TGF-β can induce the differentiation of Thl7 cells. IL-23 can promote the production of IL17, as well as participate in amplification and maintenance of the survival of IL-l7 generating cells. In this process, STAT3 and ROR-γt are key transcription factors for the growing of Thl7 cells. As our knowledge of Th17 family members is rapidly growing and changing, ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971555 Fri, 06 Nov 2009 19:47:45 +0100 2971555 http://www.springerlink.com/content/jru424647q474138/ Abstract  The aim of this paper was to investigate the prevalence of human papilloma virus (HPV) infection in women under 30 years old with cervical intraepithelial neoplasia (CIN) or cervical cancer, in order to provide a basis for cervical cancer prevention and treatment. We recruited 2052 female cases from the education system in Futian District, Shenzhen city, from April 2006 to April 2008, with age ranging from 22 to 60 years old. Secondgeneration hybrid capture test was done for detection of female genital tract HPV and cervical colposcopy for screening CIN or cervical cancer. The prevalence of HPV and cervical precancerous lesions or cervical cancer was evaluated in different age groups of women. The HPV positive rate was 247/2052 (12.00%), and 35/2052 (1.71%) of the... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971556 Fri, 06 Nov 2009 19:10:08 +0100 2971556 http://www.springerlink.com/content/a6730022q0008205/ Abstract  The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea. A total of 98 patients with stage IV cancers were recruited and divided into a prevention group (52 patients) and a control group (46 patients). All patients received the chemotherapy including irinotecan. The prevention group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days; the control group did not receive any prevention. The use of gentamycin and sodium bicarbonate resulted in significantly higher stool pH (P<0.001), while the incidence of diarrhea by irinotecan was reduced (prevention group 13.70% versus control group 34.83%; P<0.001). Gentamycin combined with sodium bi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971557 Fri, 06 Nov 2009 19:10:07 +0100 2971557 http://www.springerlink.com/content/r478m83775p50v20/ Abstract  The expression of signal transducer and activator of transcription 6 (STAT6) and nuclear factor-κB (NF-κB) in the colonic mucosa of patients with ulcerative colitis (UC) was examined. Real-time polymerase chain reaction and immunohistochemistry were used to detect the expression of STAT6 and NF-κB p65 at both mRNA and protein levels in the colonic mucosa of patients with UC and healthy volunteers. The results showed that the expression levels of STAT6 and NFκB p65 in the colonic mucosa of patients with UC were significantly higher than in normal controls at both mRNA and protein levels. These data suggest that STAT6 and NFκB p65 perhaps play an important role in the pathogenesis of UC and underscore the potential value of anti-UC strategies in the clinical ma... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2971558 Fri, 06 Nov 2009 19:10:03 +0100 2971558 http://www.springerlink.com/content/f38347wr1hkt266h/ Abstract  This paper is aimed to examine if changes in platelet-derived growth factor (PDGF) expression at different stages of cervical cancer are related to the variation in blood vessel density (BVD) and lymphatic vessel density (LVD) to evaluate the relationship between PDGF expression and stages and metastasis of cervical cancer. Polymerase chain reaction (PCR) and RT-PCR were used to detect the expression levels of PDGF in 45 cervical cancer tissue samples (the experimental group). The samples were immunohistochemically stained with monoclonal antibodies D2-40 and CD34, and BVD and LVD were measured. The expressions of PDGF-A, -B, and -D were all higher in the experimental group than in the control group (P<0.05); no significant difference was found in the expression... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968962 Thu, 05 Nov 2009 07:45:23 +0100 2968962 http://www.springerlink.com/content/n226q4261r476752/ Abstract  The effects of compound Sophorae Flavescentis Jiechangrong capsule (CSFJC) on the expression of nuclear factor-κB p65 (NF-κB p65) and signal transducer and activator of transcription 6 (STAT6) in the intestinal mucosa of patients with ulcerative colitis and the possible mechanism were investigated. Eighteen patients with ulcerative colitis were randomly divided into a traditional Chinese medicine (TCM) group (n = 11) treated by CSFJC and a western medicine (WM) group (n = 7) treated by Sulfasalazine tablets. The treatment duration lasted eight weeks. Before and after the treatment, the symptoms and the physical signs were observed, and the routine stool test, the colonoscopy, and pathological examination were performed in the two groups. The expression levels of ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968963 Thu, 05 Nov 2009 07:45:21 +0100 2968963 http://www.springerlink.com/content/w78v565532025263/ We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s11684-009-0076-8Authors Lenan Shao, Huazhong University of Science and Technology Department of Stomatology, Tongji Hospital, Tongji Medical College Wuhan 4300... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968964 Thu, 05 Nov 2009 07:45:19 +0100 2968964 http://www.springerlink.com/content/m6083632r37g3273/ Abstract  This paper is aimed to investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma GBC-SD cells. The viability of human gallbladder carcinoma GBC-SD, GBC-SD/enhanced green fluorescent protein (EGFP), and GBC-SD/survivin cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and mRNA and protein of survivin were tested by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. After the cells were treated with cisplatin (DDP) (3.0 µg/mL) for the same time, cell survival rate and IC50 was detected with MTT, cell apoptosis was detected with fluorescence-activated cell sorting (FACS), and the nuclear alteration was observed by TdT-mediated deoxyuridine triphosphate nick... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968965 Thu, 05 Nov 2009 07:45:18 +0100 2968965 http://www.springerlink.com/content/j107586317283j05/ Abstract  This paper aims to evaluate the effects and the possible mechanisms of atorvastatin on tumor growth and metastasis in a xenograft tumor model. Twenty-four female athymic BALB/C mice with MDA-MB-435 xenograft tumors were randomly assigned to three groups: a control group, a low-dose atorvastatin treatment group, and a high-dose atorvastatin treatment group. The mice in the treatment groups began to be administered with atorvastatin (10 or 20 mg/kg per day) when the xenograft tumors reached 1 cm in diameter. At the end of the experiment, the tumor volume and weight and the lung metastasis colonies of each mouse were measured. Western blotting was applied to detect phosphorylation of protein kinase B (PKB, Akt), extracellular signal regulated kinase (ERK), c-Jun N-te... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2968966 Wed, 04 Nov 2009 21:51:14 +0100 2968966 http://www.springerlink.com/content/l20536765rr6p15n/ Abstract  The aim of this study was to investigate the inhibitory effects of recombinant adenovirus vector carrying tissue inhibitor of metalloproteinase-3 (RAd-TIMP-3) against degeneration of rabbit intervertebral disc. Thirty Japanese white rabbits of 4 months old were randomly divided into 5 groups. Mild or moderate rabbit lumbar disc degeneration model was constructed with the controllable axial loading device by imposing 98 N pressure at the discs for 2 weeks. Various doses of virus were injected into the degenerated discs as follows: 20 μL of normal saline in group 1; 20 μL of RAd66 (an empty adenovirus vector, 1.0 × 1010 OPU/mL) in group 2; and 20, 10, and 5 μL of RAdTIMP-3 (1.0 × 1010 OPU/mL) in groups 3, 4, and 5, respectively. Two weeks after the injection, th... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2941341 Wed, 28 Oct 2009 07:03:24 +0100 2941341 http://www.springerlink.com/content/j4r30m15w53g7827/ Abstract  p38 regulated/activated protein kinase (PRAK) plays a key role in cell senescence and tumor suppression. The aim of this study was to investigate if PRAK had effect on cell proliferation. The growth of PRAK +/+ and PRAK −/− mouse embryonic fibroblast (MEF) cells was measured by methylthiazoletetrazolium (MTT) colorimetric assay, and the proportion of the cell number in different phases of the cell cycle was analyzed by flow cytometry. The growth curves showed that the growth rate was notably decreased, and cell double time was elongated in PRAK −/− cells; moreover, the number of PRAK −/− cells was decreased by 44.5% compared with that of PRAK +/+ cells cultured for 96 h, suggesting that G2/M transition is inhibited in PRAK −/− cells. Meanwhile, ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2936604 Tue, 27 Oct 2009 18:07:25 +0100 2936604 http://www.springerlink.com/content/2318566036757rr8/ In conclusion, exercise training ameliorates insulin resistance. The reduction of the level of serum TNF-α and the increased expression of TNF-α in adipose tissue by exercise training may be involved in this mechanism. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0071-0Authors Hui Sun, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaXiuling Deng, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaFangxi Xiao, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaLulu Chen, Huazhong University of Science and Tech... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2936605 Tue, 27 Oct 2009 18:07:24 +0100 2936605 http://www.springerlink.com/content/c8146m5157655p41/ Abstract  Natriuretic peptide precursor A (NPPA) is synthesized, stored, and released by atrial myocytes. Previous studies have shown that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis. Rs5065 NPPA gene polymorphism leads to the translation of NPPA with two additional arginines and has been suggested to be associated with salt-sensitive hypertension. The purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphism and the risk of coronary heart disease (CHD) in Chinese Han population. We genotyped the single nucleotide polymorphism (SNP) rs5065 NPPA in the human NPPA gene in 1861 sex- and age-matched subjects, comprising of 904 CHD cases and 957 controls of Chinese Han population.... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924264 Thu, 22 Oct 2009 18:41:12 +0100 2924264 http://www.springerlink.com/content/l0k1118720m25800/ Abstract  The spinal epidural space is an uncommon presenting site in primary non-Hodgkin’s lymphomas, especially for children. A boy suffered spinal cord compression syndrome caused by primary spinal epidural non-Hodgkin’s lymphoma. Thoracolumbar magnetic resonance imaging (MRI) demonstrated an intraspinal mass. An operation was performed with gross total tumor removal. Histological examination revealed a non-Hodgkin’s B-cell lymphoma. Bone marrow aspiration was negative for lymphoma involvement. No other therapies (chemotherapy and/or radiotherapy) were performed according to the parents’ opinion. The patient died approximately one year after the operation due to brain metastases. The clinical course and imaging features were discussed with a review of literatures... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924263 Thu, 22 Oct 2009 18:41:12 +0100 2924263 http://www.springerlink.com/content/u62674636t63v576/ Abstract  In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis. The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000. The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. The cell cycle was analyzed by using DNA content analysis by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain. CDK1 gene was successfully silenced by its siRNA, and the CDK1 protein expression level was... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924266 Thu, 22 Oct 2009 18:41:11 +0100 2924266 http://www.springerlink.com/content/u62616847342g711/ Abstract  Bone remodeling in orthodontic treatment has been one of the most concerned issues. The purpose of this study was to characterize the changes in bone remodeling on both labial and lingual aspects of the anterior teeth during treatment and retention. Data of 55 extraction cases (41 females and 14 males) were collected at pretreatment, post-treatment, and follow-up stages. Bone thickness on both labial and lingual aspects of the upper and lower incisors were measured at the level of the center of resistance (UC and LC) and that of 3 mm apical to the upper and lower incisors’ center of resistance (UAC and LAC). For LC, with the significant lingual movement of point LIR (center of resistance of lower incisor; P < 0.001), no statistically significant changes in alv... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2924265 Thu, 22 Oct 2009 18:41:11 +0100 2924265 http://www.springerlink.com/content/w68210xq8656022p/ This article presents a case of renal AML occurring in a 14-year-old girl with a definite diagnosis of TSC. She had been diagnosed with TSC at the age of three, and a schedule for close observation was disobeyed. At this time, she underwent a series of examinations: physical examination, ultrasonography, angiography, computed tomography (CT), and magnetic resonance imaging (MRI) scans. The physical examination showed adenoma sebaceum in a butterfly paranasal distribution, and a mass was palpated in the left upper quadrant. There were no neurological deficits. Imaging studies (including ultrasonography, angiography, CT, and MRI) of the abdomen showed a large heterogeneous mass arising from the left kidney. Partial nephrectomy was performed. The pathological diagnosis was hemorrhagic r... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2906567 Sat, 17 Oct 2009 17:22:54 +0100 2906567 http://www.springerlink.com/content/567733j010586278/ This study was to investigate the regulation of proliferation and apoptosis by LY294002, an inhibitor of PI3K in cervical cancer cells and the expression of FLICE-like inhibitory protein (c-FLIP) in vitro. Human cervical cancer HeLa cells were used in this experiment and cultured. The cultured cells were treated with LY294002 at different concentrations (10, 25, 50 and 100 μmol/L) for 6, 12, 24, and 48 h before harvesting for evaluation. Cell viability was measured by 3-(4,5)-dimethylthiazol(-2-y1)-3,5-di-phenyltetrazoliumbromide (MTT) assay. Apoptosis was analyzed by flow cytometry. The expression of c-FLIP was detected by Western blot. Cell viability was inhibited by LY294002 significantly (P<0.05). Flow cytometry analysis revealed that cell apoptosis was significantly increased... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2661648 Thu, 30 Jul 2009 10:20:23 +0100 2661648 http://www.springerlink.com/content/jq7k86p13u6n0045/ Abstract  The aim of this paper is to investigate the relationship between hepatitis B virus (HBV) DNA levels during the course and the progression to cirrhosis with chronic hepatitis B. A total of 239 chronic hepatitis B patients confirmed by liver biopsy between 2001 and 2007 were followed up for a median of 28 months. Compared with the patients without cirrhosis, the patients progressed to cirrhosis were older and with higher HBV-DNA levels at end point. However, there was no significant difference in cirrhosis progression between different HBV-DNA groups at baseline (P = 0.531). Kaplan-Meier analysis showed higher HBV-DNA level at endpoint had increasing risk of cirrhosis (P = 0.019). The results of Cox model indicated that HBV-DNA levels at endpoint, stage of fibrosis, n... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2661649 Wed, 29 Jul 2009 07:04:15 +0100 2661649 http://www.springerlink.com/content/m67038251030v122/ Abstract  The eukaryotic expression vector of human arresten gene was constructed and its secretive expression human embryonic kidney (HEK 293) cells was detected. Human arresten gene was amplified from recombinant plasmid pGEM-Arr by polymerase chain reaction (PCR), and then digested with restriction endonucleases BamH I and EcoR I. The target fragment was inserted into the BamH I and EcoR I restriction sites of eukaryotic expression vector pSecTag2 to construct pST-AT. Restriction analysis and DNA sequencing indicated that the arresten gene was successfully inserted into pSecTag2. The recombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent, and the expression of the target gene was detected. RTPCR revealed that the mRNA of the t... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643075 Sat, 25 Jul 2009 08:53:42 +0100 2643075 http://www.springerlink.com/content/m846k66152vnv662/ This study aimed to investigate the sensory innervation of the anterior eye segment in rats by retrograde tracing with 1,1-dioleyl-3,3,3,3-tetramethylindocarbocyanine, 4-chlorobenesulfonate (FAST Dil) injected into the anterior chamber. The sensory innervation of distinct elements of the anterior segment of the rat’s eye, i.e. the cornea, ciliary body, iris, and trabecular meshwork, were studied by retrograde tracing using FAST Dil as a tracer. FAST Dil was injected into the anterior chambers of the rat’s eyes. The animals were sacrificed at different time points, i.e., 2, 3, 4, and 5 days after the injection. FAST Dil localization in trigeminal ganglions was studied with a fluorescent microscope. Two days after FAST Dil injection into the anterior chambers, the cornea, the ciliar... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643076 Sat, 25 Jul 2009 04:36:09 +0100 2643076 http://www.springerlink.com/content/15703w6211872166/ Abstract  In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0057-yAuthors Xunli Xia, Huazhong University of Science and Technology China-UK HUST-RRes Genetics... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643077 Sat, 25 Jul 2009 04:12:36 +0100 2643077 http://www.springerlink.com/content/y4h01kn336p62833/ Abstract  Abstract The purpose of this article is to determine the effect of a well-designed combined aerobic, resistance, and extension exercise program on bone mineral density (BMD) in postmenopausal women. The population comprised 45 postmenopausal women, who exercised over 12 months (exercise group), and 36 women who served as a non-training control group. BMD of the hip, and lumbar spine was measured at the baseline and 12th month. Repeated measurement analysis of variance and nonparametric test were utilized to compare differences between the exercise group and controls. Thirty-six out of 45 persons in the exercise group and 36 controls completed the study. Average compliance was 82.2% for the whole exercise group at the 12th month. All the subjects had decreased BMD,... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643079 Sat, 25 Jul 2009 04:12:34 +0100 2643079 http://www.springerlink.com/content/8833rjlm1745x050/ In this study, we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion. TC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis. Western blot and enzyme-linked immunosorbent assay (ELISA) analysis showed that the TC-tagged core protein, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) could be expressed in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag into core protein in some degree, but cells transfected with the HBV vector coul... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643078 Sat, 25 Jul 2009 04:12:34 +0100 2643078 http://www.springerlink.com/content/x577786770g15607/ Abstract  The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H0 (non-transfected Hela cells), H1 (pGenesil-1 empty plasmid-transfected Hela cells), and H2 (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O2) and normoxia for 48 h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch test and the invasion assay were applied to examine the invasion in each group. The expression of HIF-1α, E-cadherin, and β-catenin in tumor grafts was assayed immunohistochemically. Western blot results revealed that the bands of HIF-1α, E-cadhe... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2643080 Sat, 25 Jul 2009 04:12:33 +0100 2643080 http://www.springerlink.com/content/m883581477347072/ This article reviews the current knowledge of viral hepatitis (types B and C) at the level of miRNA and tries to outline areas of potential studies. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-009-0055-0Authors Gang Li, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 ChinaXiaojia Xiong, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 China Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China) Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2638958 Wed, 22 Jul 2009 01:47:02 +0100 2638958 http://www.springerlink.com/content/m7331283j041n372/ Abstract  Lanthanum salt is a prescription drug, but its underlying functions and mechanisms are not fully understood currently. To explore the potential therapeutic value of lanthanum chloride, cytotoxicity test was applied to investigate its effects on cell proliferation. Furthermore, we observed its influence on pro-oncogene c-met by reverse transcription polymerase chain reaction (RT-PCR). In MCF-7 cell line, lanthanum chloride repressed cell proliferation at high concentrations but had no significant inhibition effect on cell growth at low concentrations. However, we observed that lanthanum chloride repressed c-met transcription at a low concentration. This may suggest that lanthanum chloride is a potent drug to inhibit the high expression of c-met in carcinoma cells an... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2638957 Wed, 22 Jul 2009 01:47:02 +0100 2638957 http://www.springerlink.com/content/5qrtwu42786l36p5/ Abstract  The feasibility and clinical therapeutic effects of laparoscopic sigmoid vaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser syndrome (MRKHs) were explored. The records of 11 MRKHs patients who underwent laparoscopic sigmoid vaginoplasty from 2003 to 2005 were reviewed, and long-term results were evaluated by follow-up. The mean operating time was 234 min (range, 130–300 min), the mean hospital stay was 9.4 days (range, 7–15 days), and the mean hemoglobin drop was 1.91 g/dL (range, 1.6–3.2 g/dL). A functional neovagina was created measuring 11 to 14 cm in length and two fingers in breadth in all patients. No introitus stenosis was observed. No intra-operative or postoperative bowel complication occurred. At the 3rd postoperative month, the first interc... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632315 Tue, 21 Jul 2009 15:17:42 +0100 2632315 http://www.springerlink.com/content/v463507277h51348/ Abstract  This paper aims to investigate the effects of Zhihuang (枳黄) decoction on CD14 expression in the lipopolysaccharide signal transduction pathway of alcohol-induced liver disease in rats. Seventy-five Wistar rats were randomly divided into three groups. Ethanol (56%, weight/volumn) was intragastrically administrated to 50 rats (14 mL/kg body weight per day) for 10 days to establish a model of alcohol-induced liver disease, and 25 of these 50 rats were treated with Zhihuang decoction simultaneously. Liver injury was evaluated by biochemical examination. The plasma content of endotoxin was assayed by biochemistry. The expression of CD14 mRNA and protein in rat liver was measured by reverse transcription polymerase chain reaction and immunohistochemistry, r... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632316 Tue, 21 Jul 2009 15:17:38 +0100 2632316 http://www.springerlink.com/content/c68713m76226032q/ Abstract  To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor (VEGF) and the possible mechanism of VEGF, the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group, VEGF-treated group and VEGF + N-nitro-L-arginine methyl ester (L-NAME)-treated group. The absorbance (A) value of vascular endothelial cells and the levels of prostaglandin (PGI2), endothelin-1 (ET-1) and von Willebrand factor (vWF) in the supernatant were observed by water-soluble tetrazolium salt assay, radioimmunoassay and enzyme-linked immunosorbent assay. The A values and PGI2 in VEGF-treated group and VEGF + L-NAME-treated group were higher than those in control group (P < 0.05 and P < 0.01). The ET-1 and vWF were sign... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632317 Tue, 21 Jul 2009 15:17:35 +0100 2632317 http://www.springerlink.com/content/k7g0564453x1q054/ Abstract  In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expression of the murine cdk4 gene in vitro. Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants were transfected by lipofectamine into BV-2 cells. The gene silencing efficacy rates of the 3 targets were compared by Western blotting. The cdk4-siRNA2 was the most effective i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632318 Tue, 21 Jul 2009 15:17:29 +0100 2632318 http://www.springerlink.com/content/b288046r7273m054/ Abstract  The study evaluated the value of ultrasound elastography in differentiating the benign and malignant superficial lymph nodes. A total of 112 subjects, including 82 patients with enlarged lymph nodes and 30 healthy volunteers, were recruited. All the subjects were examined by B-mode ultrasonography, power Doppler ultrasonography and elastography. Most of the patients were histopathologically confirmed by needle aspiration cytology and some patients were diagnosed by clinical data combined with follow-up findings. The sensitivity, specificity and accuracy of B-mode ultrasonography were 59.8%, 76.5% and 67.1%, those of the blood flow classification by power Doppler ultrasonography 77.0%, 82.3% and 79.4% and those of elastographic classification 74.7%, 97.1% and 84.5%... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632319 Tue, 21 Jul 2009 15:17:25 +0100 2632319 http://www.springerlink.com/content/t5158642687231k2/ Abstract  More and more molecular drugs based on targeted therapy have been utilized in the treatment of gynecologic cancer, especially in ovarian cancer. In this article, we systematically review the current targeted therapeutic trials running in clinic. Large, randomized trials have been conducted in the treatment of ovarian cancer, endometrial cancer and cervical cancer by usingsmall molecule, antisense, mutational gene as well as antibodies. Other planned or ongoing trials currently targeted at molecular markers which may play important roles in gynecological carcinogenesis and progression suggest that combination chemotherapy with molecular targeted therapy will ultimately be an important option. Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-009-0052-... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2632320 Tue, 21 Jul 2009 15:17:24 +0100 2632320 http://www.springerlink.com/content/770m278451576442/ Abstract  Lung cancer is one of the most common human cancers and the number one cancer killer in the United States. In general, lung cancer includes small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), but NSCLC accounts for approximately 90% of lung cancer. The early diagnosis and therapy of lung cancer still presents a big challenge because validated screening tools, which can improve current early detection to reduce mortality from lung cancer, do not exist. Over the last decade, molecular genetic abnormalities have been described in NSCLC, including chromosomal aberrations, overexpression of oncogenes, and deletion and/or mutations in tumor suppressor genes. These molecular markers in NSCLC demonstrated close associations with the development of lung c... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2612527 Thu, 16 Jul 2009 00:16:31 +0100 2612527 http://www.springerlink.com/content/bt76321492496555/ Abstract  Proteinase-activated receptors (PARs) are a novel subclass of seven transmembrane-spanning, G protein-coupled receptors. PAR-1 and PAR-2 are widely expressed in a variety of cells and are found to be involved in many physiological and pathological processes including inflammation and immune response. However, little is known about the function of PAR-1, 2 in acute graft vs host disease (GVHD). In the present study, we first detected the expression of PAR-1, 2 protein and mRNA in a murine model of acute GVHD using the methods of immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction (PCR). Syngeneic hematopoietic stem cell transplantation (HSCT) mice served as controls. The relative gene expression level of PAR-1 was significantly i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2604048 Tue, 14 Jul 2009 10:55:05 +0100 2604048 http://www.springerlink.com/content/3586143n46162612/ This study aimed to investigate the protective effect of nicotine on dopaminergic neurons and its mechanisms in mice with Parkinson disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). C57BL/6J mice were injected with MPTP for 8 days to establish a PD model. Nicotine was given for 10 days in the nicotine therapeutic group. Animals were examined behaviorally with the pole test and traction test. Tyrosine hydroxylase (TH) and γ-aminobutyric acid (GABA) were determined by using the immunocytochemistry (ICC) method. The ultrastructural changes of the caudate nucleus (CN) were observed under electron microscopy. The results showed that pretreatment with nicotine could improve the dyskinesia of PD mice markedly. Simultaneously, TH-positive (P<0.01) neurons and G... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2604049 Tue, 14 Jul 2009 10:55:03 +0100 2604049 http://www.springerlink.com/content/u6657131mv2561m7/ Abstract  Renal tubulointerstitial fibrosis (TIF) is the common end stage of various chronic renal diseases, and pirfenidone (PFD) is a novel, broad-spectrum anti-fibrotic compound but little is known about its effect and mechanism of action on renal TIF. In this work, we employed a unilateral ureteral obstruction (UUO) rat model to investigate the apoptosis of renal tubular epithelial cells (RTC) after PFD treatment. Thirty-five Sprague Dawley (SD) rats were randomized into three groups: sham-operated group (n = 7), UUO group (n = 14) and PFD group (n = 14). All rats were sacrificed at day 7 or 14 after operation. Renal histology was studied by using periodic acid schiff reagent (PAS) and Masson trichromic stain (MASSON); apoptosis was detected by in situ terminal deoxynucl... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2596198 Sat, 11 Jul 2009 07:36:55 +0100 2596198 http://www.springerlink.com/content/d7071rr1686814r5/ Abstract  Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×106 and 4.096×106, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients in... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2589974 Mon, 06 Jul 2009 20:04:35 +0100 2589974 http://www.springerlink.com/content/1344414037565366/ Abstract  A referral patient who had previously undergone varicose vein surgery was admitted as an emergency case. On admission, the patient complained of intolerable pain, paralysis and paresthesia of the affected limb, which was characterized by acute arterial ischemia symptoms. Color Doppler of the artery of the affected limb indicated that no blood flow signal existed in the superficial femoral artery. During exploratory operation, we found that the right superficial femoral artery instead of the great saphenous vein of the affected limb had been stripped and ligated. Therefore, the intact right great saphenous vein was taken for auto-transplantation by inverse end-to-end anastomosis to the proximal and distal residual superficial femoral artery, which resulted in gradua... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2558099 Mon, 29 Jun 2009 08:54:00 +0100 2558099 http://www.springerlink.com/content/m248r84h63163427/ Abstract  The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in oral squamous cell carcinoma (OSCC) and tongue cancer cell line Tca8113, as well as their effects on each other. We detected PTTG protein and bFGF in OSCC tissues from 56 cases using the streptavidin-biotin peroxidase (S-P) method; additionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expression in Tca8113 was examined by immuno-cytochemistry. The results were as follows: (1) Positive rates of PTTG protein and bFGF were 78.2% and 67.3% in OSCC, respectively, which were significantly higher than those in normal mucosal tissues (P<0.05). PTTG protein was significan... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2549677 Fri, 26 Jun 2009 09:57:22 +0100 2549677 http://www.springerlink.com/content/l5u162435488v7t2/ Abstract  The study aimed to detect the expression of the Th1-specific cell surface protein T cell Ig and mucin domain-containing molecule-3 (Tim-3) mRNA in peripheral blood lymphocytes isolated from asthmatic patients and to examine the correlation among Tim-3 mRNA, interleukin-4 (IL-4), interferon-γ (IFN-γ) level, and FEV1/FVC (force expiratory volume in one second/forced vital capacity) to explore the role of Tim-3 in the development and progression of asthmatic inflammation. Tim-3 mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The IL-4 and IFN-γ levels were determined by using enzyme-linked immunosorbent assay (ELISA). The correlation among Tim-3 mRNA, IL-4, IFN-γ level, and pulmonary ventilatory capacity was analyzed. The ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2408273 Sat, 09 May 2009 05:54:27 +0100 2408273 http://www.springerlink.com/content/j2g6g79300801655/ This study aimed to investigate the expression of protease-activated receptors (PARs) on platelets in healthy individuals and preliminarily elucidate physiological functions of PARs. Thirty healthy volunteers, who did not take any anticoagulants and antiplatelet agents within 10 days before the examination, were recruited. Fasting venous blood (5 mL) was taken from the medial cubital vein in each individual and platelet-rich plasma (PRP) was prepared. The expression of PAR1 mRNA and PAR4 mRNA in PRP was determined by RT-PCR analysis. The results showed that the average levels of PAR1 mRNA and PAR4 mRNA on platelets in healthy individuals were 0.1601 ± 0.0269 and 0.1073 ± 0.0194 respectively. In combination with literature analysis, it was concluded that the thrombin signaling pathw... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371467 Sun, 26 Apr 2009 05:52:04 +0100 2371467 http://www.springerlink.com/content/r1mk246559j37812/ Abstract  The mRNA and protein expression of phosphatase of regenerating liver 1 (PRL-1) and phosphatase of regenerating liver 3 (PRL-3) in transitional cell carcinoma of bladder (BTCC) and normal epithelia of bladder was investigated, and the relationship between the BTCC and pathological changes was clarified. The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) in 30 cases of BTCC and 10 cases of normal bladder, and the expression of PRL-1 and PRL-3 protein was checked by using immunohistochemistry in 30 cases of BTCC and 15 cases of normal bladder. The expression levels of PRL-1 and PRL-3 mRNA and protein were higher in BTCC than those in normal bladder epithelia (P<0.05). The increased expression of PRL... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371468 Sun, 26 Apr 2009 05:52:03 +0100 2371468 http://www.springerlink.com/content/1754019v6988gmnt/ Abstract  To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2 (Adanti-ERK2) gene therapy upon chronic allograft nephropathy (CAN) of rats, male Lewis (LEW, RT11) rats received male Fisher (F344, RT11v1) renal allografts. The recipients were divided into three groups: (1) empty control group; (2) vector control group; (3) gene therapy group. All recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation. Morphometric analysis was used to determine the fibrosis of grafts. Immunohistochemistry was used to detect the expression of E-Cadherin, Vimentin, TβR I and the infiltration of CD4+ T lymphocyte, CD8+ T lymphocyte and ED-1+ monocytes. Enzyme linked immunosorbent assay (ELISA) was used to detect TG... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371470 Sun, 26 Apr 2009 05:52:02 +0100 2371470 http://www.springerlink.com/content/98054g13h3q7j31u/ Abstract  Rab proteins and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome to trans-Golgi-network trafficking. To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed lentivirus encoding Rab9 cDNA. The expressing plasmid pCDH1-MCF1-Rab9-EF1-copGFP was constructed by using molecular biological techniques. The lentivirus encoding Rab9 cDNA was packed by Lifectamine-2000 mediated co-transfection of the plasmid pPACKH1-GAG, pPACKH1-REV and pVSV-G into 293T cells. DNA sequencing proved the successful construction of pCDH1-MCF1-Rab9-EF1-copGFP. After 72 h, the expression of GFP could be detected in BV-2 cells. Western blotting revealed that the Rab9... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2371469 Sun, 26 Apr 2009 05:52:02 +0100 2371469 http://www.springerlink.com/content/n543p01518023512/ Abstract  Our previous studies showed that there were close relationships between connexin 43 (Cx43) and acupoints and meridians. In order to further investigate the effect of Cx43 in acupuncture treatment, RNA interference technique was used to construct small hairpin RNA (shRNA) expression vectors targeting Cx43 and identify the efficiency of RNA interference in NIH/3T3 cell lines for further use in vivo. Aiming directly at the two targets of Cx43 mRNA sequence of the rat and mouse homology region, we synthesized two pairs of complementary oligonucleotide strands in vitro. Double strands were formed after annealing, and then inserted into Pgenesil-1 plasmid expression vector. After identification by enzyme cutting and sequencing, the recombinant plasmids named P-Cx43-shRNA... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2366347 Thu, 23 Apr 2009 08:04:47 +0100 2366347 http://www.springerlink.com/content/l76t151q42647ur5/ Abstract  To clarify the effect of DNA methylation and histone deacetylase inhibitors on the expression of the E-cadherin gene and the invasiveness of the QBC939 cells, the QBC939 cells were separately treated with hydralazine, valproate, or combination of the two drugs. The expression of E-cadherin mRNA and protein was examined by reverse transcription-polymerase chain reaction (RT-PCR), and Western blot.The methylation status of the promoter region of the gene was detected by methylation-specific PCR (MSP). The invasiveness of QBC939 cells was tested by transwell assay. It was found that the promoter region of the E-cadherin gene of QBC939 cells was hypermethylated. Valproate alone could not contribute to demethylation of the gene, whereas hydralazine could make them to be ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358119 Tue, 21 Apr 2009 10:48:33 +0100 2358119 http://www.springerlink.com/content/k271h6185k4377n3/ This study indicates that EMT and expressions of VEGF, MMP-9 and COX-2, and MVD value are strongly correlated with lymph node metastasis in breast cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0038-1Authors Lihui Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaLianhong Li, Dalian Medical University Department of Pathology Dalian 116044 ChinaShen Lv, The Second Affiliated Hospital of Dalian Medical University Center Laboratory Dalian 116027 ChinaShujun Fan, Dalian Medical University Department of Pathology Dalian 116044 ChinaLi Zhan, Dalian Maternity Hospital Department of Pathology Dalian 116012 ChinaBo Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaZhong Zhang, Dalian Medical University Depa... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2491596 Tue, 21 Apr 2009 10:48:32 +0100 2491596 http://www.springerlink.com/content/k271h6185k4377n3/ This study indicates that EMT and expressions of VEGF, MMP-9 and COX-2, and MVD value are strongly correlated with lymph node metastasis in breast cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0038-1Authors Lihui Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaLianhong Li, Dalian Medical University Department of Pathology Dalian 116044 ChinaShen Lv, The Second Affiliated Hospital of Dalian Medical University Center Laboratory Dalian 116027 ChinaShujun Fan, Dalian Medical University Department of Pathology Dalian 116044 ChinaLi Zhan, Dalian Maternity Hospital Department of Pathology Dalian 116012 ChinaBo Wang, Dalian Medical University Department of Pathology Dalian 116044 ChinaZhong Zhang, Dalian Medical University Depa... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358133 Tue, 21 Apr 2009 10:48:32 +0100 2358133 http://www.springerlink.com/content/u011m1w4279503x7/ In this study, 100 women who were enrolled were randomly divided into two groups. In one group, the TCT for cervical cells was applied (TCT group), and in the other group routine cervical smear was used. In addition, the cells in the TCT group were screened by double sifters, and centrifuged using a separation medium so as to eliminate mucus, inflammatory cells and blood cells. According to the cell distribution and the thickness of the smear, the results were assigned to three groups, including satisfactory smears, less satisfactory smears and unsatisfactory smears. The TCT had a higher satisfactory rate (98%) compared to the routine cervical smear (32%) (P<0.01), indicating the TCT was superior to the routine cervical smear. It is concluded that the TCT is more acceptable. Meanwh... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358126 Tue, 21 Apr 2009 10:48:32 +0100 2358126 http://www.springerlink.com/content/a643776235m438t4/ Abstract  The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associated virus (AAV) in vitro was investigated. Recombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinant AAV genome was replicated and packaged with the helper virus HSV1-rc/ΔUL2. The titer of the recombinant rAAV2-tet-off-bFGF was determined by dot-blot assay. MC3T3-E1 cells were infected with rAAV2-tet-off-bFGF. Regulatory effects of Doxycycline (Dox) on bFGF and osteogenic factors were assayed quantitatively by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The physical particle titer of rAAV2-tet-off-bFGF successfully constructed was 1.8×1012 vector genomes/mL, and the virus could i... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358147 Tue, 21 Apr 2009 10:48:30 +0100 2358147 http://www.springerlink.com/content/j2585g964gj773w1/ The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0–100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions (P < 0.05 vs control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation (P < 0.05 vs control) but had no effect on NO pr... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358140 Tue, 21 Apr 2009 10:48:30 +0100 2358140 http://www.springerlink.com/content/h1764433m3l5164l/ In conclusion, single ovarian stimulation may produce more oocytes/embryos. However, repeated gonadotropin stimulation may have a negative effect on the ovarian follicular quality, the number of mature retrieved oocytes, and the embryo quality, even increasing the chance of ovarian cancer. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0037-2Authors Linlin Liang, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College Wuhan 430030 ChinaBei Xu, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College Wuhan 430030 ChinaGuijin Zhu, Huazhong University of Science and Technology Reproductive Medicine Center, Tongji Hospital, Tongji Medicine... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2358154 Tue, 21 Apr 2009 10:48:28 +0100 2358154 http://www.springerlink.com/content/m37557727443g540/ Abstract  The purpose of this study was to investigate the expression of inducible nitric oxide synthase(iNOS) in trophoblasts and deciduas in early medical abortion, and study the relationship of medical abortion through mifepristone and iNOS in early pregnancy. Expression of iNOS in trophoblasts and deciduas was detected by both in situ hybridization and immunohistochemical assay in 40 patients (experimental group); the positive expression of iNOS was represented by number density (N/S) and positive unit (Pu) using computer color magic image analysis system (CMIAS). All results were compared with those obtained from vacuum aspiration. In the experimental group, N/S and Pu in trophoblasts were 0.120±0.010 and 15.3±2.6, respectively, while in the control group, they were 0... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2334700 Tue, 14 Apr 2009 06:13:54 +0100 2334700 http://www.springerlink.com/content/d340600068417055/ Abstract  Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormone deficiency. To simplify the injection process and increase drug compliance, application of the GH injection has become a new treatment plan in recent years. The purpose of the current study was to evaluate the efficacy and safety of rhGH injection for the treatment of growth hormone deficiency (GHD) in children in China. In a nationwide, noncomparative, prospective, randomized, open trial, 31 children with confirmed complete GHD received subcutaneous injection of rhGH at 0.25 mg/kg·wk (0.107 IU/kg·d). The injection was given daily and the total weekly amount was separated into 6–7 injections. The patients were followed up at 3-month intervals and the treatm... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2334701 Tue, 14 Apr 2009 06:13:53 +0100 2334701 http://www.springerlink.com/content/r48hh3j5784m173w/ Abstract  We implemented a new protocol—multiphase dynamic helical scan to acquire CT angiography (CTA) and whole brain CT perfusion (CTP) images simultaneously with single scan on 16 multidetector CT (MDCT). A total of 90 patients who were randomly assigned into 3 groups were included in our study. Each group underwent CT scan by using the new protocol, traditional CTA and CTP protocol, respectively. The image quality of CTA, the CTP parameter values and the X-ray doses were measured and compared between the new protocol and the traditional protocols. There was no statistically significant difference in the CTA image quality between the above methods (P = 0.55). For CTP parameters, the new protocol tended to overestimate the blood volume (BV) and blood flow (BF) value, an... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311212 Fri, 03 Apr 2009 06:48:43 +0100 2311212 http://www.springerlink.com/content/g41915j84r22143p/ Abstract  This retrospective study was performed to compare the outcome of thoracoabdominal incision versus flank incision for radical nephrectomy in the patients with large renal tumors. A questionnaire assessing postoperative pain, administration of pain medications and the return to activities and work was sent to the patients who underwent radical nephrectomy through the 11th rib (group 1: undergoing flank incision, including 96 patients) or the 9th to 10th rib (group 2: undergoing thoracoabdominal incision, including 98 patients) from 2003 to 2007 in our hospital. A case retrospective analysis assessing operation time, perioperative hemorrhage volume, size of tumor, success in the treatment of tumor thrombus in renal vein or vena cava, time length of presence of drainag... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311214 Fri, 03 Apr 2009 06:48:42 +0100 2311214 http://www.springerlink.com/content/y77x1p3851547876/ Abstract  In order to provide a sensitive cell line model for investigating the mechanisms underlying the lymphatic metastasis of tumors and the effect of medicine against cells, a new murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L6) was established and the effect of curcumin on biological behavior of Hca-P/L6 was observed. Murine ascites hepatocarcinoma cell strain with low lymphatic metastatic potential (Hca-P) was subcutaneously inoculated into the medioventral line of a mouse 615 and the first generation of metastatic tumor cells of inguinal lymph node (Hca-P/L1) was obtained. Then, Hca-P/L1 was screened by the route of mouse foot pad subcutaneously → lymph node → scale-up culture in vitro → mouse foot pad subcutaneously f... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311218 Fri, 03 Apr 2009 06:48:41 +0100 2311218 http://www.springerlink.com/content/056482m487770543/ Abstract  To gain a broader appreciation of the clinical presentation, operative treatment, and outcome of fibrous dysplasia involving the calvarium in children, we retrospectively reviewed a series of cases of fibrous dysplasia involving the calvarium (4 males and 2 females) with patients’ age ranging from 5 to 12 years old. The clinical manifestation, radiographic findings, surgical treatment, outcome and follow-up were evaluated on the basis of medical records. Fibrous dysplasia in the series was monostotic, involving frontal bone (2 cases), temporal bone (1 case), parietal bone (2 cases) and occipital bone (1 case). The patients most commonly presented with enlarging mass and cosmetic complaints. The treatment given, depending on clinical presentation, was simple biop... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311216 Fri, 03 Apr 2009 06:48:41 +0100 2311216 http://www.springerlink.com/content/862017334460j651/ We reported a case of diffuse cavernous hemangioma involving both skin and muscles of the left lower limb and perineal region in an adolescent patient. The patient who had previously undergone a local hemangioma resection of the foot ultimately ended in superficial femoral artery ligation and supergenual thigh amputation of the left upper leg because of uncontrollable massive bleeding of anastomotic stoma. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s11684-009-0031-8Authors Yin Xia, Huazhong University of Science and Technology Department of Vascular Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaDan Shang, Huazhong University of Science and Technology Department of Vascular Surgery, Union Hospital, Tongji Medical College Wuhan 430022 ChinaQin Li,... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311215 Fri, 03 Apr 2009 06:48:41 +0100 2311215 http://www.springerlink.com/content/7825l58757m4gr82/ Abstract  To investigate the regulation of tumor suppressor XAF1 gene expression in digestive system cancers, we studied XAF1 gene promoter transcription activity and mRNA level in digestive system cancer cell lines (human hepatoma cell line HepG2, human colon cancer cell line LoVo, and human gastric cancer cell line AGS) and nontumor cell lines (human embryonic liver cell line L02 (L02 cells) and human embryonic kidney 293 cells [HEK293 cells]) as controls. 1395-bp-promoter fragment of XAF1 gene was amplified by polymerase chain reaction (PCR) and cloned into pGL3-basic vector and pEGFP-1 vector to assay its promoter transcription activity. The plasmids were transfected into a variety of cell lines by lipofectamine 2000. The promoter transcription activity was determined b... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311219 Fri, 03 Apr 2009 06:48:39 +0100 2311219 http://www.springerlink.com/content/63ln4853605010x6/ Abstract  To evaluate the mechanism of vascular endothelial growth factor (VEGF) on the prevention of restenosis after angioplasty, the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells (VSMC) in vitro. The conditioned medium containing VEGF was collected 72 h after the infection. Then, the VSMC and human umbilical vein endothelial cells (HUVEC) were divided into control group, H2O2-treated group and H2O2+ VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium (WST-1) method, in situ nick end labeling (TUNEL) and flow cytometry (FCM). Compared with the control and H2O2+ VEGF-treated groups, the absorbance (A) value of HUVEC was decreased, and apoptosis of HUVEC was signifi... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311221 Sun, 29 Mar 2009 05:53:13 +0100 2311221 http://www.springerlink.com/content/m777tt658n4224n1/ Abstract  The purpose of our research was to evaluate the efficacy, tolerance, and safety of oxcarbazepine (OXC) as monotherapy and add-on therapy for partial epilepsy. We carried out a prospective clinical follow-up trial at the Epilepsy Center of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Sixty-seven patients with partial epilepsy received OXC therapy. The patients were randomly divided into a monotherapy group and an add-on therapy group. We observed the efficacy and safety in the first three months and the following three months respectively, and compared them with each other. There was a significant difference in the decrease of seizure frequency between the two groups (P = 0.002). There was a significant difference in the pe... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2311222 Sun, 29 Mar 2009 05:53:12 +0100 2311222 http://www.springerlink.com/content/15566430x2156411/ In this study, survival fraction at 2Gy (SF2) values of eight human tumor cell lines (including four human cervical carcinoma cell lines HeLa, SiHa, C33A, Caski, three human breast carcinoma cell lines MCF-7, MDA-MB-231, MDA-MB-453, and one human lung carcinoma cell line A549) were acquired by clone formation assay, and western blot was applied to detect the expressions of Ku80, DNA-PKcs and ATM protein. The correlativity of protein expression with SF2 value was analyzed by Pearson linear correlation analysis. We found that the expression of the same protein in different cell lines and the expression of three proteins in the same cell line had a significant difference. The SF2 values were also different in eight tumor cell lines and there was a positive correlativity between the exp... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278670 Tue, 17 Mar 2009 07:02:51 +0100 2278670 http://www.springerlink.com/content/g347566582500466/ Abstract  Reactive oxygen species (ROS) are small molecule metabolites of oxygen that are prone to participate in redox reactions via their high reactivity. Intracellular ROS could be generated in reduced nicotinamide-adenine dinucleotidephosphate (NADPH) oxidase-dependent and/or NADPH oxidase-independent manners. Physiologically, ROS are involved in many signaling cascades that contribute to normal processes. One classical example is that ROS derived from the NADPH oxidase and released in neurotrophils are able to digest invading bacteria. Excessive ROS, however, contribute to pathogenesis of various human diseases including cancer, aging, dimentia and hypertension. As signaling messengers, ROS are able to oxidize many targets such as DNA, proteins and lipids, which may be ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278672 Tue, 17 Mar 2009 07:02:48 +0100 2278672 http://www.springerlink.com/content/x6814u08v7t658g6/ Abstract  The aim of this paper was to explore the relationship between energy metabolism and the meridian phenomenon. The manner of change of oxygen partial pressure in acupoints and no-acupoints of the urinary bladder meridian of goats was observed with a needle-type tissue oxygen tension sensor after the acupuncture effect was blocked by Ca2+ complexation with ethylenediamine tetraacetic acid (EDTA)-Na2. The results showed that: (1) the concentration of calcium ion in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01 or P<0.05); (2) the tissue oxygen tension in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01); (3) the oxygen partial pressure in acupoints decreased significantly after injection with EDTA-... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278678 Tue, 17 Mar 2009 07:02:47 +0100 2278678 http://www.springerlink.com/content/f4501641662878r1/ Abstract  To study the efficacy of Fuganling granula (FGL, 复肝灵颗粒) in treating mouse immunological hepatic injury that was caused by Bacille Calmette Guerin (BCG) and lipopolysaccharide (LPS), a total of 60 mice were adopted, among which, 50 mice were given intraperitoneal injection with BCG and LPS to establish an immunological liver injury model and then were randomly divided into 5 groups (10 mice/group): 4 groups received treatment of FGL orally at the doses of 100 mg/kg (high-dosage), 50 mg/kg (middle-dosage), 25 mg/kg (low-dosage) and bifendate orally at the dose of 80 mg/kg, respectively. One group was treated with distilled water orally. The remaining 10 mice were given distilled water intraperitoneally as the normal control group. T... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278676 Tue, 17 Mar 2009 07:02:47 +0100 2278676 http://www.springerlink.com/content/y73rn7lr66088605/ Abstract  The aim of this study was to explore the regulatory mechanism of retinoic acid (RA) on the TBX1 gene expression in myocardial cells. Ventricular cardiocytes were isolated from neonatal rats and cultured, and then treated with different concentrations of retinoic acid. The expression of Shh and Fgf8 at mRNA and protein levels in neonatal rat myocardial cells were measured by using RT-PCR and Western blot technique, respectively. There was basal expression of Shh and Fgf8 in the control group. When treated with 3 × 10−7 mol/L RA, we observed that the expression of Shh mRNA and protein in neonatal rat myocardial cells were up-regulated by 1.51 (P < 0.05) and 1.10 times (P < 0.05), respectively. In comparison with the control group, under the concentration of ... Frontiers of Medicine in China journals http://www.medworm.com/rss/comments.php?id=2278674 Tue, 17 Mar 2009 07:02:47 +0100 2278674