MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Genetics and Molecular Research' source. Thu, 09 Feb 2012 09:43:37 +0100 http://www.medworm.com/index.php?rid=5653993&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290460%26dopt%3DAbstract Combined analysis of the MspI and XbaI polymorphisms in intron 22 of the factor VIII gene for detection of hemophilia A in a Korean population. Genet Mol Res. 2012;11(1):1-9 Authors: Park SH, Chung N, Lee MR, Yoo SK, Choi YM Abstract To determine the usefulness of MspI/int22h-1 (intron 22 homologous region-1) polymorphism of the factor VIII gene for molecular genetic diagnosis of hemophilia A in the Korean population, MspI/intron 22 and XbaI/intron 22 polymorphisms were analyzed in 101 unrelated Korean families with severe hemophilia A. The expected heterozygosity rates of MspI/int22h-1 and XbaI/int22h-1 polymorphisms were 49.5 and 43.6%, respectively; these polymorphisms were not in complete linkage disequilibrium. Combined analysis using both polymorphisms provided an inf... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653993 Fri, 03 Feb 2012 11:24:05 +0100 5653993 http://www.medworm.com/index.php?rid=5653992&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290461%26dopt%3DAbstract Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers. Genet Mol Res. 2012;11(1):10-6 Authors: Guidugli MC, Ferreira-Ramos R, de Sousa AC, Cidade FW, Marconi TG, Mestriner MA, Groppo M, Alzate-Marin AL Abstract Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests. This tree is useful for reforesting degraded areas and the wood can be employed in construction. We developed 12 microsatellite markers from a genomic library enriched for GA/CA repeats, for this species. Polymorphisms were assessed in 40 trees of a highly fragmented population found in Cravinhos, State of São Paulo, in... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653992 Fri, 03 Feb 2012 11:24:05 +0100 5653992 http://www.medworm.com/index.php?rid=5653991&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290462%26dopt%3DAbstract Bayesian inference to study genetic control of resistance to gray leaf spot in maize. Genet Mol Res. 2012;11(1):17-29 Authors: Balestre M, Von Pinho RG, Brito AH Abstract Gray leaf spot (GLS) is a major maize disease in Brazil that significantly affects grain production. We used Bayesian inference to investigate the nature and magnitude of gene effects related to GLS resistance by evaluation of contrasting lines and segregating populations. The experiment was arranged in a randomized block design with three replications and the mean values were analyzed using a Bayesian shrinkage approach. Additive-dominant and epistatic effects and their variances were adjusted in an over-parametrized model. Bayesian shrinkage analysis showed to be an excellent approach to handle complex m... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653991 Fri, 03 Feb 2012 11:24:05 +0100 5653991 http://www.medworm.com/index.php?rid=5653990&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290463%26dopt%3DAbstract Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. Genet Mol Res. 2012;11(1):30-41 Authors: Latif MA, Omar MY, Tan SG, Siraj SS, Ali ME, Rafii MY Abstract Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653990 Fri, 03 Feb 2012 11:24:05 +0100 5653990 http://www.medworm.com/index.php?rid=5653989&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290464%26dopt%3DAbstract Molecular characteristics and expression of calmodulin cDNA from the freshwater pearl mussel, Hyriopsis schlegelii. Genet Mol Res. 2012;11(1):42-52 Authors: Zeng LG, Wang JH, Li YJ, Sheng JQ, Gu Q, Hong YJ Abstract Calmodulin (CaM) is a multifunctional intracellular calcium ion receptor protein that participates in a range of cellular processes, including calcium metabolism in mussels. To investigate the role of CaM in freshwater mollusk shell calcium metabolism, the full-length CaM cDNA was isolated from the freshwater pearl mussel, Hyriopsis schlegelii (referred to as hsCaM) using SMART RACE technology. The full-length hsCaM was 855 bp in size, containing a 70-bp 5ꞌ-untranslated sequence, a 447-bp open reading frame, a 309-bp 3ꞌ-untranslated sequence, and a 26-nucleot... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653989 Fri, 03 Feb 2012 11:24:05 +0100 5653989 http://www.medworm.com/index.php?rid=5653988&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290465%26dopt%3DAbstract An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus. Genet Mol Res. 2012;11(1):53-60 Authors: Hu Y, Zhang WL, Xie SL, Zhao Y, Hu JL, Cai XF, Lai GQ, Huang AL Abstract Early detection of adefovir dipivoxil-resistant mutants during long-term treatment of chronic hepatitis B virus (HBV) infection with this drug is of great clinical importance. We developed an improved reverse dot hybridization test for simple and rapid detection of the rtA181V/T and rtN236T mutations associated with adefovir dipivoxil resistance in chronic hepatitis B patients. Probes were designed for genotypes B, C, and D of this resistance characteristic; a total of 70 clinical samples were analyzed with this improved reverse dot hybridizati... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653988 Fri, 03 Feb 2012 11:24:05 +0100 5653988 http://www.medworm.com/index.php?rid=5653987&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290466%26dopt%3DAbstract Polymorphisms of the TIM-1 gene are associated with rheumatoid arthritis in the Chinese Hui minority ethnic population. Genet Mol Res. 2012;11(1):61-9 Authors: Xu JR, Yang Y, Liu XM, Sun JY, Wang YJ Abstract The T-cell immunoglobulin and mucin domain 1 (TIM-1) is known to be associated with susceptibility to rheumatoid arthritis (RA). We investigated the association of four single-nucleotide polymorphisms (SNPs) in the promoter region of the TIM-1 gene with susceptibility to RA in a Chinese Hui ethnic minority group. Using RFLP or sequence specific primer-PCR, 118 RA patients and 118 non-arthritis control individuals were analyzed for the -1637A>G, -1454G>A, -416G>C, and -232A>G SNPs in the TIM-1 gene. The polymorphisms -232A>G and -1637A>G in the promoter... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653987 Fri, 03 Feb 2012 11:24:05 +0100 5653987 http://www.medworm.com/index.php?rid=5653986&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290467%26dopt%3DAbstract We examined the frequency of the most relevant variants encoding CYP2C9 (alleles *1, *2 and *3) and VKORC1 (SNP -1639A>G) in the Argentinian population. Molecular typing was performed by PCR-RFLP on a randomly selected sample of 101 healthy volunteers from the Hospital Italiano de Buenos Aires gene bank. Fifty-seven subjects were identified as homozygous for CYP2C9*1 and 14 for *2, while 24 and 5 were heterozygous for *2 and *3 alleles; one individual was a composite heterozygote (*2/*3). When we examined VKORC1, 21 subjects were AA homozygous, 60 were AG heterozygotes and 20 were GG homozygotes. This is the first analysis of genotypic frequencies for CYP2C9 and VKORC1 performed in an Argentinian population. These allele prevalences are similar to what is known for Caucasian population,... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653986 Fri, 03 Feb 2012 11:24:05 +0100 5653986 http://www.medworm.com/index.php?rid=5653985&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290468%26dopt%3DAbstract Sodium valproate inhibits MDA-MB-231 breast cancer cell migration by upregulating NM23H1 expression. Genet Mol Res. 2012;11(1):77-86 Authors: Li GF, Qian TL, Li GS, Yang CX, Qin M, Huang J, Sun M, Han YQ Abstract Breast cancer is a common cancer in women, with a highly variable course, from inoffensive to lethal. To find a more effective strategy for its treatment, sodium valproate has been tested as an anti-cancer drug; it is the only clinically available histone deacetylase inhibitor. However, data about the effects of sodium valproate on breast cancer are insufficient in both animals and humans; studies have yielded conflicting conclusions. In particular, little is known about the association between expression of the metastasis suppressor Nm23H1 gene and breast cancer. ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653985 Fri, 03 Feb 2012 11:24:05 +0100 5653985 http://www.medworm.com/index.php?rid=5653984&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290469%26dopt%3DAbstract Association of T869C gene polymorphism of transforming growth factor-β1 with low protein levels and anthropometric indices in osteopenia/osteoporosis postmenopausal Thai women. Genet Mol Res. 2012;11(1):87-99 Authors: Utennam D, Tungtrongchitr A, Phonrat B, Tungtrongchitr R, Preutthipan S Abstract Osteoporosis is the most common metabolic bone disease; it is an important health problem among postmenopausal women. We evaluated the association of three polymorphisms, T869C, C-509T and G915C, of the TGF-β1 gene with bone mineral density (BMD) serum TGF-β1 levels in 278 postmenopausal female osteopenia/osteoporosis subjects and 95 postmenopausal female control subjects. Serum TGF-β1 levels were significantly lower in osteopenia/osteoporosis subjects than in control subjects... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653984 Fri, 03 Feb 2012 11:24:05 +0100 5653984 http://www.medworm.com/index.php?rid=5653983&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290470%26dopt%3DAbstract We examined the geno-, cyto- and overall toxicity of P. niruri whole plant ethanolic extract. The extract was administered as a single dose of 30 or 300 mg/kg to laboratory rats by gavage, accompanied by negative (0.9% saline) and positive (10 mg/mL N-ethyl-N-nitrosourea) controls that were injected intramuscularly 48 h after extract administration. The ratio of polychromatic (PCE)/normochromatic erythrocytes (NCE) from femur bone marrow was scored for genotoxicity. Cytotoxicity was determined using descending concentrations (0.2-0.0125 g/mL) of the extract incubated with peripheral blood mononuclear cells. Lactate dehydrogenase release from damaged cells was determined and the CC(50) calculated. Subchronic administration of the extract at 30 or 300 mg/kg was done for 90 days to determine ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653983 Fri, 03 Feb 2012 11:24:05 +0100 5653983 http://www.medworm.com/index.php?rid=5653982&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290471%26dopt%3DAbstract Abnormal male meiosis explains pollen sterility in the polyploid medicinal plant Pinellia ternata (Araceae). Genet Mol Res. 2012;11(1):112-20 Authors: Liu Y, Hui RK, Deng RN, Wang JJ, Wang M, Li ZY Abstract Pinellia ternata is an important traditional Chinese medicinal plant. Its different populations in China have various ploidy levels, based on x = 13, as well as extensive aneuploid series. The microsporogenesis process was observed in specimens from three populations from three regions of Hubei Province; they were characterized by normal and abnormal meiotic divisions in pollen mother cells (PMCs) at all stages simultaneously. Meiotic abnormalities including univalents/multivalents, chromosomal laggards/bridges and micronuclei appeared in about 50% of the PMCs, together ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653982 Fri, 03 Feb 2012 11:24:05 +0100 5653982 http://www.medworm.com/index.php?rid=5653981&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290472%26dopt%3DAbstract Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata. Genet Mol Res. 2012;11(1):121-30 Authors: Guan JZ, Wang JJ, Cheng ZH, Liu Y, Li ZY Abstract Houttuynia cordata (Saururaceae) is a leaf vegetable and a medicinal herb througout much of Asia. Cytomixis and meiotic abnormalities during microsporogenesis were found in two populations of H. cordata with different ploidy levels (2n = 38, 96). Cytomixis occurred in pollen mother cells during meiosis at high frequencies and with variable degrees of chromatin/chromosome transfer. Meiotic abnormalities, such as chromosome laggards, asymmetric segregation and polyads, also prevailed in pollen mother cells at metaphase of the first division an... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653981 Fri, 03 Feb 2012 11:24:05 +0100 5653981 http://www.medworm.com/index.php?rid=5653980&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290473%26dopt%3DAbstract We examined the cytogenetics of mithun (Bos frontalis), a domesticated version of the Asian gaur, and hybrids (F(1) generation) produced by artificial insemination of Brahman cows (Bos indicus) with mithun semen. Reproductive potential was also examined in the F(1) generation and a backcrossed heifer for utilization of heterosis. Metaphase chromosome spreads were examined by conventional staining and fluorescence in situ hybridization hybridized with the entire chromosome 1 of mithun as a specific probe. Chromosome 1 of mithun was found to be equivalent to Bos taurus chromosomes 2 and 28. The karyotype of the female mithun (N = 4) comprised 58 chromosomes, including 54 acrocentric and four large submetacentric chromosomes, without the four acrocentric chromosomes found in the domesticated ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5653980 Fri, 03 Feb 2012 11:24:05 +0100 5653980 http://www.medworm.com/index.php?rid=5544091&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194162%26dopt%3DAbstract Back fat thickness and meat tenderness are associated with a 526 T→A mutation in the exon 1 promoter region of the MyF-5 gene in Chinese Bos taurus. Genet Mol Res. 2011;10(4):3070-9 Authors: Ujan JA, Zan LS, Ujan SA, Adoligbe C, Wang HB Abstract Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live weight and meat characteristics in indigenous Chinese cattle breeds. PCR-SSCP methodology showed a T>A mutation at 526 bp. Least square analysis revealed a ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544091 Wed, 28 Dec 2011 02:00:11 +0100 5544091 http://www.medworm.com/index.php?rid=5544090&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194163%26dopt%3DAbstract RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil. Genet Mol Res. 2011;10(4):3080-9 Authors: Souza IG, Valente SE, Britto FB, de Souza VA, Lima PS Abstract We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material using a commercial purification kit, was subjected to PCR with the primers A01, A09, G03, G10, N05, and M16. Fifty-five polymorphic loci were identified, with mean of 9.16 ± 3.31 bands per primer and 100% polymorphism. Application of unweighted pair group method using ar... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544090 Wed, 28 Dec 2011 02:00:11 +0100 5544090 http://www.medworm.com/index.php?rid=5544089&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194164%26dopt%3DAbstract Detection of novel SNPs and mapping of the fatness QTL on pig chromosome 7q1.1-1.4 region. Genet Mol Res. 2011;10(4):3090-7 Authors: Huang WH, Ma ZX, Xu ZY, Xiong YZ, Zuo B Abstract Many QTLs for fatness traits have been mapped on pig chromosome 7q1.1-1.4 in various pig resource populations. Eight novel markers, including seven SNPs and one insertion or deletion within BTNL1, COL21A1, PPARD, GLP1R, MDFI, GNMT, ABCC10, and PLA2G7 genes, as well as two previously reported SNPs in SLC39A7 and HMGA1 genes, were genotyped in Large White and Meishan pig breeds. Except for two SNPs in HMGA1 and ABCC10 genes, allele frequencies of the other eight markers are highly significant different between Chinese indigenous Meishan breeds and Large White pig breeds. Eight polymorphic sites we... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544089 Wed, 28 Dec 2011 02:00:11 +0100 5544089 http://www.medworm.com/index.php?rid=5544088&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194165%26dopt%3DAbstract Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas. Genet Mol Res. 2011;10(4):3098-108 Authors: Lu Y, Xu WH, Xie YX, Zhang X, Pu JJ, Qi YX, Li HP Abstract Commercial banana varieties are highly susceptible to fungal pathogens, as well as bacterial pathogens, nematodes, viruses, and insect pests. The largest known family of plant resistance genes encodes proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense. Six degenerate PCR primers were designed to target NBS and additional domains were tested on comme... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544088 Wed, 28 Dec 2011 02:00:11 +0100 5544088 http://www.medworm.com/index.php?rid=5544087&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194166%26dopt%3DAbstract In conclusion, the positive allele of HLA-Cw*0602 polymorphism appears to be a risk factor for psoriasis. PMID: 22194166 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544087 Wed, 28 Dec 2011 02:00:11 +0100 5544087 http://www.medworm.com/index.php?rid=5544086&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194167%26dopt%3DAbstract We examined polymorphism of the TCTA tetranucleotide sequence in the 3rd intron of the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene in the Han population of Ningxia Province in China. We also looked for a possible relationship between STR polymorphism in the 3rd intron of the HPRT gene and primary hyperuricemia. We used Chelex-100 to extract DNA, then PCR, PAGE and silver staining for allele genotyping and DNA sequencing to obtain the distribution of the alleles. We found, for the first time, that there is high STR polymorphism in the 3rd intron of the HPRT gene. We detected 5 STR alleles in this intron in the Han population of Ningxia Province, with 15 genotypes in females; significant differences were observed in the distribution of alleles and genotypes between control and... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544086 Wed, 28 Dec 2011 02:00:11 +0100 5544086 http://www.medworm.com/index.php?rid=5544085&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194168%26dopt%3DAbstract Genetic parameter estimates and response to selection for weight and testicular traits in Nelore cattle. Genet Mol Res. 2011;10(4):3127-40 Authors: Araujo Neto FR, Lôbo RB, Mota MD, Oliveira HN Abstract We estimated genetic parameters for various phases of body and testicular growth until 550 days of age in Nelore cattle, using Bayesian inference, including correlation values and error estimates. Weight and scrotal records of 54,182 Nelore animals originating from 18 farms participating in the Brazilian Nelore Breeding Program (PMGRN) were included. The following traits were measured: weight at standard ages of 120 (W120), 210 (W210), 365 (W365), 450 (W450), and 550 (W550) days; weight gain between 120/210 (WG1), 210/365 (WG2), 365/450 (WG3), 450/550 (WG4), 120/365 (WG5), ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544085 Wed, 28 Dec 2011 02:00:11 +0100 5544085 http://www.medworm.com/index.php?rid=5544084&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194169%26dopt%3DAbstract Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species. Genet Mol Res. 2011;10(4):3141-5 Authors: Xuan SQ, Zheng JY, Wang H, Gao P, Zhou SB, Liu K, Zhu GP Abstract Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci from 21 L. radiata individuals of a natural population from Yellow Mountain in Anhui Province, China. The number of alleles ranged from two... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544084 Wed, 28 Dec 2011 02:00:11 +0100 5544084 http://www.medworm.com/index.php?rid=5544083&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194170%26dopt%3DAbstract Genetic markers of body composition and carcass quality in grazing Brangus steers. Genet Mol Res. 2011;10(4):3146-56 Authors: Baeza MC, Corva PM, Soria LA, Rincon G, Medrano JF, Pavan E, Villarreal EL, Schor A, Melucci L, Mezzadra C, Miquel MC Abstract The somatotropic axis is a major regulatory pathway of energy metabolism during postnatal growth in mammals. Genes involved in this pathway influence many economically important traits. The association of selected SNPs in these genes with carcass traits was examined in grazing Brangus steers. These traits included final live weight, ultrasound backfat thickness (UBFT), rib-eye area, kidney fat weight, hot carcass weight, and intramuscular fat percentage (%IMF). Genomic DNA (N = 246) was genotyped for a panel of 15 tag SNPs lo... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544083 Wed, 28 Dec 2011 02:00:11 +0100 5544083 http://www.medworm.com/index.php?rid=5544082&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194171%26dopt%3DAbstract XPD and hOGG1 gene polymorphisms in reperfusion oxidative stress. Genet Mol Res. 2011;10(4):3157-62 Authors: Isbir O, Görmüş U, Ergen HA, Cakmakoğlu B, Kahraman OT, Baykan N Abstract Knee replacement surgery is an ischemia/reperfusion model, as it uses tourniquet applied to the knee area to stop the blood flow during the operation. Fifty patients that were undergoing elective arthroscopic knee surgery were included in our study. Human 8-oxoguanine glycosylase 1 (hOGG1) is an enzyme to repair specific DNA lesions and a good marker of hydroxyl radical damage to DNA. XPD is another DNA repair gene. We investigated the effect of hOGG1 (Ser326Cys) and XPD (Lys751Gln) polymorphisms on the oxidative stress level after reperfusion. To evaluate oxidative stress conditions, we me... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544082 Wed, 28 Dec 2011 02:00:11 +0100 5544082 http://www.medworm.com/index.php?rid=5544081&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194172%26dopt%3DAbstract Sulcus vocalis: evidence for autosomal dominant inheritance. Genet Mol Res. 2011;10(4):3163-8 Authors: Martins RH, Gonçalves TM, Neves DS, Fracalossi TA, Tavares EL, Moretti-Ferreira D Abstract We found evidence of autosomal dominant hereditary transmission of sulcus vocalis. Four dysphonic patients from three generations of the same family were submitted to videolaryngoscopic examination (three patients) and to direct laryngoscopy (one patient) to diagnose the hoarseness. Sulcus vocalis was diagnosed in all four patients. The finding of four affected individuals in three generations, with vertical transmission affecting man and women, is more consistent with autosomal dominant inheritance pattern; it is an etiological model that we propose for the sulcus vocalis in this p... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544081 Wed, 28 Dec 2011 02:00:11 +0100 5544081 http://www.medworm.com/index.php?rid=5544080&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194173%26dopt%3DAbstract In vitro antioxidant activities of endophytic fungi isolated from the liverwort Scapania verrucosa. Genet Mol Res. 2011;10(4):3169-79 Authors: Zeng PY, Wu JG, Liao LM, Chen TQ, Wu JZ, Wong KH Abstract We investigated in vitro antioxidant activities of 49 endophytic fungi isolated from the liverwort Scapania verrucosa. Based on morphological and molecular identification, the endophytic fungi isolated were classified into seven genera (Hypocrea, Penicillium, Tolypocladium, Chaetomium, Xylaria, Nemania, and Creosphaeria), all belonging to one family (Xylariaceae). By screening with the 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) decolorization assay, the ethyl acetate extracts of five endophytic fungi (T7, T21, T24, T32, and T38 strains), which exhibited remark... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544080 Wed, 28 Dec 2011 02:00:11 +0100 5544080 http://www.medworm.com/index.php?rid=5544079&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194174%26dopt%3DAbstract Methodology Adaptation of a low-cost medium-throughput genotyping system for ovine prion protein gene polymorphims associated with scrapie. Genet Mol Res. 2011;10(4):3180-5 Authors: Ianella P, McManus CM, Paiva SR, Caetano AR Abstract Resistance and susceptibility to scrapie in sheep have been associated with SNPs located at codons 136, 154 and 171 of the prion protein (PRNP) gene. Many countries have sheep breeding programs selecting for resistance to scrapie based on the genotyping of these SNPs. We adapted a fast and robust method for genotyping sheep flocks for these polymorphisms, with reduced costs. Ninety-six samples were genotyped using an adapted SNaPshot PRNP assay, and the results were checked by resequencing. The results showed 100% concordance, using a method t... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544079 Wed, 28 Dec 2011 02:00:11 +0100 5544079 http://www.medworm.com/index.php?rid=5544078&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194175%26dopt%3DAbstract Population genetic structure of the tropical tree species Aegiphila sellowiana (Lamiaceae). Genet Mol Res. 2011;10(4):3186-98 Authors: Medri C, Ruas EA, Ruas CF, Medri PS, Medri ME, Ruas PM Abstract The Tibagi River, located in southern Brazil, is associated with a significant degree of environmental heterogeneity, along its 550 km extension. There is concern about the integrity of this river's ecosystem, as human interference has been increasing. Aegiphila sellowiana (Lamiaceae) is an important pioneer tree species, commonly found near rivers; the fruit is consumed by avifauna. We studied this species along three ecological gradients, comprising the upper, middle, and lower regions of the Tibagi River basin. The genetic structure of nine subpopulations of A. sellowiana dis... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544078 Wed, 28 Dec 2011 02:00:11 +0100 5544078 http://www.medworm.com/index.php?rid=5544077&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194176%26dopt%3DAbstract Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle. Genet Mol Res. 2011;10(4):3199-203 Authors: Huang JM, Wang ZY, Ju ZH, Wang CF, Li QL, Sun T, Hou QL, Hang SQ, Hou MH, Zhong JF Abstract Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the splice variants of the bLF gene in the mammary gland, spleen and liver tissues. One main transcript corresponding to the bLF reference sequence was found in three tissues in both healthy and mastit... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544077 Wed, 28 Dec 2011 02:00:11 +0100 5544077 http://www.medworm.com/index.php?rid=5544076&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194177%26dopt%3DAbstract Methodology Molecular cloning and characterization of gene coding for γ-tocopherol methyltransferase from lettuce (Lactuca sativa). Genet Mol Res. 2011;10(4):3204-12 Authors: Tang YL, Ren WW, Zhang L, Tang KX Abstract γ-tocopherol methyltransferase is an important rate-limiting enzyme involved in tocopherol biosynthesis. The full-length cDNA encoding γ-tocopherol methyltransferase (designated as LsTMT) was cloned from Lactuca sativa for the first time by rapid amplification of cDNA ends and characterized by means of quantitative RT-PCR. The full-length cDNA of LsTMT was 1131 bp, with an open reading frame of 897 bp encoding a γ-tocopherol methyltransferase protein of 298 amino acids, with a calculated molecular mass of 33.06 kDa and an isoelectric point of 5.86. Compara... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544076 Wed, 28 Dec 2011 02:00:11 +0100 5544076 http://www.medworm.com/index.php?rid=5544075&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194178%26dopt%3DAbstract Evaluation of HPFH and δβ-thalassemia mutations in a Brazilian group with high Hb F levels. Genet Mol Res. 2011;10(4):3213-9 Authors: Carrocini GC, Ondei LS, Zamaro PJ, Bonini-Domingos CR Abstract Fetal hemoglobin (Hb F) is characteristic of the fetal development period. However, in some genetic conditions, such as hereditary persistence of fetal hemoglobin (HPFH) and delta-beta thalassemia (δβ-thalassemia), Hb F continues to be produced in adulthood. We evaluated the frequency of two mutations of HPFH, HPFH-1 and HPFH-2 African, and two mutations in δβ-thalassemia, Sicilian and Spanish, in a Brazilian population. Peripheral blood samples were collected from adults from hospitals and blood centers in southeast and northeast Brazil. These individuals were healthy and w... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544075 Wed, 28 Dec 2011 02:00:11 +0100 5544075 http://www.medworm.com/index.php?rid=5544074&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194179%26dopt%3DAbstract We examined the relationship between pyrethroid resistance and gene transcription levels of two closely related genes, CYP6P9 and CYP6P13, in FUMOZ-R. Levels of resistance to 0.75% permethrin were determined based on standard WHO insecticide susceptibility assays using females and males of different ages, ranging from 3 to 30 days old. The transcription levels of the two genes were quantified using qPCR for each age cohort. In the WHO insecticide susceptibility assays, survival of both males and females significantly decreased as age increased. Quantitative analysis of the two genes CYP6P9 and CYP6P13 showed the highest levels of expression at 10 days of age. There was no correlation between expression of these two genes and pyrethroid survival by age. We conclude that the resistance of th... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544074 Wed, 28 Dec 2011 02:00:11 +0100 5544074 http://www.medworm.com/index.php?rid=5544073&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194180%26dopt%3DAbstract Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae). Genet Mol Res. 2011;10(4):3230-5 Authors: Wang Y, Lu H, Zheng J, Long K, Shi YH, Gu ZF, Wang AM Abstract The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per locus, expected and observed heterozygosities ranging from 0.68 to 0.94 and 0.56 to 0.81, respectively. One locus (HUBA09) showed significant deviation from Hardy-Weinberg equilibrium, probably due to the presenc... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544073 Wed, 28 Dec 2011 02:00:11 +0100 5544073 http://www.medworm.com/index.php?rid=5544072&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194187%26dopt%3DAbstract Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts. Genet Mol Res. 2011;10(4):3236-45 Authors: Costa WF, Oliveira AB, Nepomuceno JC Abstract The search for new and effective antitumor agents with fewer cytotoxic side effects on normal tissue has increasingly become important. Lapachol, a natural organic compound isolated from the lapacho tree (Tabebuia avellandedae), is chemically identified as belonging to the naphthoquinone group and is known for its anti-inflammatory, analgesic and antibiotic properties, although there are questions about its effectiveness for treating neoplasic cells. We evaluated the antitumoral effects of lapachol by testing for clones of epithelial tumors in Drosophila melanogaster. S... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544072 Wed, 28 Dec 2011 02:00:11 +0100 5544072 http://www.medworm.com/index.php?rid=5544071&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194188%26dopt%3DAbstract Characterization of 12 microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae), and cross-amplification in five other lamprey species. Genet Mol Res. 2011;10(4):3246-50 Authors: Spice EK, Whitesel TA, McFarlane CT, Docker MF Abstract The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is of conservation concern in North America and Asia. Data on Pacific lamprey population structure are scarce and conflicting, impeding conservation efforts. We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three to 13 alleles per locus were observed in a sample of 51 fish collected from the West Fork Illinois River, Oregon. Observed heterozygosity ranged from 0.235 to 0.902 and expected heterozygosity ranged from 0.21... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544071 Wed, 28 Dec 2011 02:00:11 +0100 5544071 http://www.medworm.com/index.php?rid=5544070&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194189%26dopt%3DAbstract A rapid protocol for purification of total RNA for tissues collected from pigs at a slaughterhouse. Genet Mol Res. 2011;10(4):3251-5 Authors: Méndez V, Avelar E, Morales A, Cervantes M, Araiza A, González D Abstract Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughterhouses. This protocol, modified from other techniques, uses TRIzol Reagent. Sample collection was performed wearing sterile gloves and facemasks, using sterile surgical instruments, and no longer than 8 min spent for each sample. A 0.9% ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544070 Wed, 28 Dec 2011 02:00:11 +0100 5544070 http://www.medworm.com/index.php?rid=5544069&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194190%26dopt%3DAbstract S323I polymorphism of the C5L2 gene was not identified in a Chinese population with familial combined hyperlipidemia or with type 2 diabetes. Genet Mol Res. 2011;10(4):3256-66 Authors: Zheng YY, Xie X, Ma YT, Yang YN, Fu ZY, Li XM, Liu F, Yang SJ, Ma X, Chen BD Abstract C5L2, a G protein-coupled receptor, is known to be a functional receptor of acylation-stimulating protein, which is a stimulator of triglyceride synthesis and glucose transport. A novel C5L2 variant (S323I) was identified and its association with familial combined hyperlipidemia (FCH) was recently reported. We looked for this SNP in three Chinese ethnic groups, including Han, Uygur, and Kazakh controls and patients with FCH and type 2 diabetes. One hundred and eighty-two unrelated subjects (77 of Han, 57 of ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544069 Wed, 28 Dec 2011 02:00:11 +0100 5544069 http://www.medworm.com/index.php?rid=5544068&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22194191%26dopt%3DAbstract Genetic loci mapping associated with maize kernel number per ear based on a recombinant inbred line population grown under different nitrogen regimes. Genet Mol Res. 2011;10(4):3267-74 Authors: Liu XH, He SL, Zheng ZP, Tan ZB, Li Z, He C Abstract Kernel number per ear (KNE) is one of the most important yield-related agronomic traits in maize (Zea mays). To clarify its genetic basis, we made a quantitative trait locus (QTL) analysis of KNE in a recombinant inbred line population derived from lines Mo17 and Huangzao4, under two nitrogen (N) regimes. Seven QTLs, on chromosomes 4, 6 and 9, were mapped under the high N regime, which explained phenotypic variation ranging from 5.03 to 15.49%. Under the low N regime, three QTLs were located on chromosomes 6 and 9, which accounted ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5544068 Wed, 28 Dec 2011 02:00:11 +0100 5544068 http://www.medworm.com/index.php?rid=5524578&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179959%26dopt%3DAbstract Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata). Genet Mol Res. 2011;10(4):2867-80 Authors: Liu SJ, Chen FF, Dai Y, Wu C, Ni QS, Yu WY Abstract The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the MDIi gene in various tissues by real-time quantitative PCR. A full-length cDNA sequence of MDIi was obtained, 1188-bp long, encoding a 222-amino acid protein. A comparison of the amino ac... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524578 Thu, 22 Dec 2011 03:54:02 +0100 5524578 http://www.medworm.com/index.php?rid=5524577&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179960%26dopt%3DAbstract Detection of two morphologically cryptic species from the cursor complex (Akodon spp; Rodentia, Cricetidae) through the use of RAPD markers. Genet Mol Res. 2011;10(4):2881-92 Authors: Yazbeck GM, Brandão RL, Cunha HM, Paglia AP Abstract The cursor complex is a group within the Akodon genus of South American rodents, formed by Akodon cursor and A. montensis. Correct distinction between these two species is of great importance since they can harbor different Hantavirus strains. These species are only distinguishable by means of karyotypic or internal anatomic features, requiring dissection; recently, some other genetic methods have become available. We developed RAPD markers capable of distinguishing between A. cursor and A. montensis. Samples included 42 individuals of A. c... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524577 Thu, 22 Dec 2011 03:54:02 +0100 5524577 http://www.medworm.com/index.php?rid=5524576&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179961%26dopt%3DAbstract Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers. Genet Mol Res. 2011;10(4):2893-904 Authors: Lima AT, de Souza VA, Gomes RL, Lima PS Abstract The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in preliminary tests, and primers were selected from a set designed by the University of British Columbia on the basis of high levels of polymorphism and adequate band resolution. The 21 primers employed in the final analys... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524576 Thu, 22 Dec 2011 03:54:02 +0100 5524576 http://www.medworm.com/index.php?rid=5524575&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179962%26dopt%3DAbstract Novel NKX2-5 mutations responsible for congenital heart disease. Genet Mol Res. 2011;10(4):2905-15 Authors: Wang J, Liu XY, Yang YQ Abstract Congenital heart disease (CHD) is the most common birth defect and is the leading cause of infant morbidity and mortality resulting from birth defects. Increasing evidence demonstrates that genetic variation in the NKX2-5 gene, which encodes a homeobox-containing transcription factor crucial to cardiogenesis, is an important molecular determinant for CHD. Nevertheless, the genetic components underlying CHD remain largely unknown. We screened NKX2-5 for potential molecular defects in patients with CHD. The entire coding region of NKX2-5 was initially sequenced in a cohort of 268 unrelated patients with CHD. The relatives of the patients... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524575 Thu, 22 Dec 2011 03:54:02 +0100 5524575 http://www.medworm.com/index.php?rid=5524574&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179963%26dopt%3DAbstract Transferability and utility of white oat (Avena sativa) microsatellite markers for genetic studies in black oat (Avena strigosa). Genet Mol Res. 2011;10(4):2916-23 Authors: Da-Silva PR, Milach SC, Tisian LM Abstract Preservation and use of wild oat species germplasm are essential for further improvement of cultivated oats. We analyzed the transferability and utility of cultivated (white) oat Avena sativa (AACCDD genome) microsatellite markers for genetic studies of black oat A. strigosa (AsAs genome) genotypes. The DNA of each black oat genotype was extracted from young leaves and amplified by PCR using 24 microsatellite primers developed from white oat. The PCR products were separated on 3% agarose gel. Eighteen microsatellite primer pairs amplified consistent products and... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524574 Thu, 22 Dec 2011 03:54:02 +0100 5524574 http://www.medworm.com/index.php?rid=5524573&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179964%26dopt%3DAbstract We examined whether p53 codon 72 polymorphism confers prostate cancer risk by conducting a meta-analysis. Two investigators independently searched the Pubmed, Embase and CBM databases. This meta-analysis was made of seven case-control studies, that included 892 prostate cancer cases and 1020 healthy controls. Meta-analysis results based on all the studies showed no significant association between p53 codon 72 polymorphism and prostate cancer risk in the comparisons of Pro allele vs Arg allele; Pro/Pro + Pro/Arg vs Arg/Arg; Pro/Pro vs Pro/Arg + Arg/Arg; Pro/Pro vs Arg/Arg, and Pro/Arg vs Arg/Arg [odds ratio (OR) = 1.09, 95% confidence interval (CI) = 0.87-1.36, P = 0.47; OR = 1.22, 95%CI = 0.86-1.73, P = 0.27; OR = 1.03, 95%CI = 0.62-1.72, P = 0.91; OR = 1.22, 95%CI = 0.66-2.26, P = 0.52; O... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524573 Thu, 22 Dec 2011 03:54:02 +0100 5524573 http://www.medworm.com/index.php?rid=5524572&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179965%26dopt%3DAbstract Evaluation of genetic diversity in Piper spp using RAPD and SRAP markers. Genet Mol Res. 2011;10(4):2934-43 Authors: Jiang Y, Liu JP Abstract Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analysis were applied to 74 individual plants of Piper spp in Hainan Island. The results showed that the SRAP technique may be more informative and more efficient and effective for studying genetic diversity of Piper spp than the RAPD technique. The overall level of genetic diversity among Piper spp in Hainan was relatively high, with the mean Shannon diversity index being 0.2822 and 0.2909, and the mean Nei's genetic diversity being 0.1880 and 0.1947, calculated with RAPD and SRAP data, respectively. The ranges of the genetic similarity coeffic... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524572 Thu, 22 Dec 2011 03:54:02 +0100 5524572 http://www.medworm.com/index.php?rid=5524571&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179966%26dopt%3DAbstract Phenotypic and genetic parameters compared during repeated measures of longissimus muscle area and subcutaneous fat thickness in Nelore cattle. Genet Mol Res. 2011;10(4):2944-52 Authors: Pinheiro TR, Mercadante ME, Albuquerque LG, Cyrillo JN, Branco RH Abstract Real-time ultrasound is currently used for in vivo carcass evaluation of beef cattle. We estimated heritability and repeatability coefficients for ultrasound-measured carcass traits in Nelore cattle. We measured longissimus muscle area, backfat thickness, and rump fat thickness in male and female animals ranging in age from 10 to 26 months. The variance components were estimated by single-trait analysis using the derivative-free restricted maximum likelihood method, under an animal model. The complete data file inclu... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524571 Thu, 22 Dec 2011 03:54:02 +0100 5524571 http://www.medworm.com/index.php?rid=5524570&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179967%26dopt%3DAbstract Molecular cytogenetic characterization of a new leaf rolling triticale. Genet Mol Res. 2011;10(4):2953-61 Authors: Yang EN, Yang ZJ, Zhang JF, Zou YC, Ren ZL Abstract Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed protein showed that CMH83 was genetically stable. Sequential Giemsa-C-banding and genomic in situ hybridization showed that CMH83 contains 12 rye chromosomes; two pairs of these chromosomes have reduced telomeric heterochromatin bands. Tests of relative water content and water loss rate of l... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524570 Thu, 22 Dec 2011 03:54:02 +0100 5524570 http://www.medworm.com/index.php?rid=5524569&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179968%26dopt%3DAbstract The effects of resveratrol on cyclooxygenase-1 and -2, nuclear factor kappa beta, matrix metalloproteinase-9, and sirtuin 1 mRNA expression in hearts of streptozotocin-induced diabetic rats. Genet Mol Res. 2011;10(4):2962-75 Authors: Yar AS, Menevse S, Alp E Abstract Resveratrol (RSV) has a beneficial role in the prevention of diabetes and alleviates some diabetic complications, such as cardiomyopathy. We investigated cyclooxygenase-1 (COX-1), COX-2, nuclear factor κB (NF-κB), matrix metalloproteinase-9 (MMP-9), and sirtuin 1 (SIRT1) mRNA expression levels in heart tissue after RSV treatment in streptozotocin (STZ)-induced diabetic rats. After induction of chronic diabetes with STZ, 10 mg RSV/kg per day was administered to DM and DM+RSV groups for four weeks. At the end o... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524569 Thu, 22 Dec 2011 03:54:02 +0100 5524569 http://www.medworm.com/index.php?rid=5524568&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180031%26dopt%3DAbstract Genetic diversity in tetraploid switchgrass revealed by AFLP marker polymorphisms. Genet Mol Res. 2011;10(4):2976-86 Authors: Todd J, Wu YQ, Wang Z, Samuels T Abstract Switchgrass (Panicum virgatum) is a perennial warm-season grass native to North America that has been identified as a dedicated cellulosic biofuel crop. We quantified genetic diversity in tetraploid switchgrass germplasm collected at Oklahoma State University and characterized genetic relatedness among the collections from distinct regions. Fifty-six tetraploid accessions, including seven upland and 49 lowland genotypes from throughout the US, were examined. The amplified fragment length polymorphism (AFLP) procedure was utilized to generate DNA profiling patterns that were scored visually. Sixteen selective ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524568 Thu, 22 Dec 2011 03:54:02 +0100 5524568 http://www.medworm.com/index.php?rid=5524567&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180032%26dopt%3DAbstract Neuroprotective effects of NEP1-40 and fasudil on Nogo-A expression in neonatal rats with hypoxic-ischemic brain damage. Genet Mol Res. 2011;10(4):2987-95 Authors: Zhu WW, Ma XL, Guo AL, Zhao HY, Luo HH Abstract The hypoxic-ischemic encephalopathy caused by peripartum asphyxia is a serious disease in newborn infants, and effective therapies need to be developed to reduce injury-related disorders. We evaluated the effects of NEP1-40 and fasudil on Nogo-A expression in neonatal hypoxic-ischemic brain damage (HIBD) rats. Seven-day-old Wistar rats were randomly divided into control, HIBD, NEP1-40, and fasudil groups. NEP1-40 and fasudil groups were injected intraperitoneally with these compounds. Rat brains at 6, 24, 72 h, and 7 days after HIBD were collected to determine histo... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524567 Thu, 22 Dec 2011 03:54:02 +0100 5524567 http://www.medworm.com/index.php?rid=5524566&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180033%26dopt%3DAbstract Analysis of glutathione peroxidase 1 gene polymorphism and Keshan disease in Heilongjiang Province, China. Genet Mol Res. 2011;10(4):2996-3001 Authors: Wei HL, Pei JR, Jiang CX, Zhou LW, Lan T, Liu M, Wang T Abstract Keshan disease (KD) is an endemic cardiomyopathy associated with selenium deficiency. Recent studies indicate that glutathione peroxidase 1 (GPx1) mutation decreases GPx activity in myocardial cells and increases the risk of KD. To further clarify the correlation between GPx1 polymorphism and KD, we analyzed GPx1 polymorphism, blood selenium levels and GPx activity in KD patients and healthy controls in Heilongjiang Province. Four and 24 new mutation loci in the promoter and the exon region, respectively, of the GPx1 gene were found in the subjects, in contrast... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524566 Thu, 22 Dec 2011 03:54:02 +0100 5524566 http://www.medworm.com/index.php?rid=5524565&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180034%26dopt%3DAbstract Carnitine and carnitine orotate affect the expression of the prolactin-releasing peptide gene. Genet Mol Res. 2011;10(4):3013-9 Authors: Zhu HS, Wang YY, Lin MW, Du JX, Hang LQ, Chen Y, Wang LF Abstract Carnitine is involved in fatty acid metabolism in mammals and is widely used as a nutritional supplement; carnitine orotate is a more absorbable form of carnitine. We investigated the effects of carnitine and carnitine orotate on mouse prolactin-releasing peptide (PrRP) mRNA expression. Twenty-four female mice were randomly divided into four groups of six; control mice were orally drenched with physiological saline solution (250 mg/kg body weight) and treatment mice were orally drenched with carnitine (250 mg/kg) or carnitine orotate (250 or 750 mg/kg), once a day, for 20 da... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524565 Thu, 22 Dec 2011 03:54:02 +0100 5524565 http://www.medworm.com/index.php?rid=5524564&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180035%26dopt%3DAbstract Simple method for culture of peripheral blood lymphocytes of Testudinidae. Genet Mol Res. 2011;10(4):3020-5 Authors: Silva TL, Silva MI, Venancio LP, Zago CE, Moscheta VA, Lima AV, Vizotto LD, Santos JR, Bonini-Domingos CR, Azeredo-Oliveira MT Abstract We developed and optimized a simple, efficient and inexpensive method for in vitro culture of peripheral blood lymphocytes from the Brazilian tortoise Chelonoidis carbonaria (Testudinidae), testing various parameters, including culture medium, mitogen concentration, mitotic index, culture volume, incubation time, and mitotic arrest. Peripheral blood samples were obtained from the costal vein of four couples. The conditions that gave a good mitotic index were lymphocytes cultured at 37°C in minimum essential medium (7.5 mL), ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524564 Thu, 22 Dec 2011 03:54:02 +0100 5524564 http://www.medworm.com/index.php?rid=5524563&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180036%26dopt%3DAbstract Transposable elements in Phyllostachys pubescens (Poaceae) genome survey sequences and the full-length cDNA sequences, and their association with simple-sequence repeats. Genet Mol Res. 2011;10(4):3026-37 Authors: Zhou MB, Liu XM, Tang DQ Abstract Phyllostachys pubescens is a woody bamboo with the highest ecological, economic, and cultural values of all bamboos in Asia. There is more genomic data available for P. pubescens than for any other bamboo species, including 2.12-Mb genome survey sequences (GSS) and 11.4-Mb full-length cDNA sequences (FL-cDNAs) currently deposited in GenBank. Analysis of these sequences revealed that transposable elements (TEs) are abundant, diverse and polyphyletic in the P. pubescens genome, of which Ty3-gypsy and Ty1-copia are the two most abund... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524563 Thu, 22 Dec 2011 03:54:02 +0100 5524563 http://www.medworm.com/index.php?rid=5524562&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180037%26dopt%3DAbstract Genetic polymorphism of the glutathione-S-transferase P1 gene (GSTP1) and susceptibility to prostate cancer in the Kashmiri population. Genet Mol Res. 2011;10(4):3038-45 Authors: Qadri Q, Sameer AS, Shah ZA, Hamid A, Alam S, Manzoor S, Siddiqi MA Abstract Glutathione-S-transferase P1 (GSTP1) is a critical enzyme of the phase II detoxification pathway. One of the common functional polymorphisms of GSTP1 is A→G at nucleotide 313, which results in an amino acid substitution (Ile105Val) at the substrate binding site of GSTP1 and reduces catalytic activity of GSTP1. To investigate the GSTP1 Ile105Val genotype frequency in prostate cancer cases in the Kashmiri population, we designed a case-control study, in which 50 prostate cancer cases and 45 benign prostate hyperplasia case... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524562 Thu, 22 Dec 2011 03:54:02 +0100 5524562 http://www.medworm.com/index.php?rid=5524561&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180038%26dopt%3DAbstract Generation of transgenic chicks using an oviduct-specific expression system. Genet Mol Res. 2011;10(4):3046-55 Authors: Kaleri HA, Xu SY, Lin HL Abstract We successfully replaced the ovalbumin gene of a magnum region in chickens with a human plasminogen activator. We constructed pL-eGFP, pL-tPAGFP and pL-2.8OVtPAGFP vectors and cultured 293FT chicken embryo fibroblasts, chicken primordial germ cells, Hela C127 cells, and oviduct epithelial cells. All vectors were expressed in the transfected cells, except pL-2.8OVtPAGFP vector, which was only expressed in oviduct epithelial cells. A lentivirus with pL-2.8OVtPAGFP was injected in fertilized eggs; 11 chicks hatched in the G(0) generation, four of them carried the tPAGFP. Two cockerels from the G(0) generation were crossed wit... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524561 Thu, 22 Dec 2011 03:54:02 +0100 5524561 http://www.medworm.com/index.php?rid=5524560&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180039%26dopt%3DAbstract An overview of odorant-binding protein functions in insect peripheral olfactory reception. Genet Mol Res. 2011;10(4):3056-69 Authors: Fan J, Francis F, Liu Y, Chen JL, Cheng DF Abstract Insect olfactory perception involves many aspects of insect life, and can directly or indirectly evoke either individual or group behaviors. Insect olfactory receptors and odorant-binding proteins (OBPs) are considered to be crucial to insect-specific and -sensitive olfaction. Although the mechanisms of interaction between OBPs or OBP/ligand complex with olfactory receptors are still not well understood, it has been shown that many OBPs contribute to insect olfactory perception at various levels. Some of these are numerous and divergent members in OBP family; expression in the olfactory orga... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524560 Thu, 22 Dec 2011 03:54:02 +0100 5524560 http://www.medworm.com/index.php?rid=5524546&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183944%26dopt%3DAbstract Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients. Genet Mol Res. 2011 Dec 8;10(4) Authors: Viana-Baracioli LM, Tukamoto Junior NC, Ricci Júnior O, Mattos LC, Angulo IL, Bonini-Domingos CR Abstract It is well documented that Hb S and iron affect blood cells, and trigger oxidative processes and generation of free radicals with potential for lipid peroxidation. We evaluated the frequency of polymorphisms in the HFE gene in Hb AS blood donors and how these polymorphisms influenced lipid peroxidation and antioxidant capacity. Blood samples were collected from 211 Hb AS blood donors, 119 Hb AA blood donors as a control group, and 28 sickle cell disease patients (Hb SS). The ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524546 Thu, 08 Dec 2011 05:00:00 +0100 5524546 http://www.medworm.com/index.php?rid=5524545&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183945%26dopt%3DAbstract Genetic diversity of sea-island cotton (Gossypium barbadense) revealed by mapped SSRs. Genet Mol Res. 2011 Dec 8;10(4) Authors: Wang XQ, Feng CH, Lin ZX, Zhang XL Abstract In order to evaluate the genetic diversity of sea-island cotton (Gossypium barbadense), 237 commonly mapped SSR markers covering the cotton genome were used to genotype 56 sea-island cotton accessions. A total of 218 polymorphic primer pairs (94.75%) amplified 361 loci, with a mean of 1.66 loci. Polymorphism information content values of the SSR primers ranged from 0.035 to 0.862, with a mean of 0.320. The highest mean polymorphism information content value for the SSR motifs was from a compound motif (0.402), and for the chromosomes it was Chr10 (0.589); the highest ratio of polymorphic primers in Xinjia... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524545 Thu, 08 Dec 2011 05:00:00 +0100 5524545 http://www.medworm.com/index.php?rid=5524544&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183946%26dopt%3DAbstract Resistance to lipopolysaccharide-induced endotoxic shock in heterozygous Zfp191 gene-knockout mice. Genet Mol Res. 2011 Dec 8;10(4) Authors: Sun RL, Wang HY, Yang XY, Sheng ZJ, Li LM, Wang L, Wang ZG, Fei J Abstract Zinc finger protein 191, ZNF24 and Zfp191 in both humans and mice belong to the SCAN domain subfamily of Krüppel-like zinc finger transcription factors. Previous studies have suggested that Zfp191 is a pleiotropic factor involved in embryonic development, hematopoiesis and tumorigenesis. However, little is known about its target genes or its role in other physiological and pathological processes. We have identified the putative target genes of Zfp191, using an in silico genome-wide scan. Three hundred and fifty-five putative target genes were identified, which ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524544 Thu, 08 Dec 2011 05:00:00 +0100 5524544 http://www.medworm.com/index.php?rid=5524543&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183947%26dopt%3DAbstract Methylation status and chromatin structure of the myostatin gene promoter region in the sea perch Lateolabrax japonicus (Perciformes). Genet Mol Res. 2011 Dec 8;10(4) Authors: Abbas EM, Takayanagi A, Shimizu N, Kato M Abstract Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain, and heart) isolated from adult specimens. The frequency of methylated cytosines was very low in all tissues, regardless of the level of myostatin expression, s... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524543 Thu, 08 Dec 2011 05:00:00 +0100 5524543 http://www.medworm.com/index.php?rid=5524542&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183948%26dopt%3DAbstract Comparison of bacterial communities in the Solimões and Negro River tributaries of the Amazon River based on small subunit rRNA gene sequences. Genet Mol Res. 2011 Dec 8;10(4) Authors: Peixoto JC, Leomil L, Souza JV, Peixoto FB, Astolfi-Filho S Abstract The microbiota of the Amazon River basin has been little studied. We compared the structure of bacterial communities of the Solimões and Negro Rivers, the main Amazon River tributaries, based on analysis of 16S rRNA gene sequences. Water was sampled with a 3-L Van Dorn collection bottle; samples were collected at nine different points/depths totaling 27 L of water from each river. Total DNA was extracted from biomass retained by a 0.22-μm filter after sequential filtration of the water through 0.8- and 0.22-μm filters. T... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524542 Thu, 08 Dec 2011 05:00:00 +0100 5524542 http://www.medworm.com/index.php?rid=5524541&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183949%26dopt%3DAbstract We examined the role of tumor-associated chondroitin sulfate in breast cancer progression. Enzymatic elimination of endogenous chondroitin sulfate by intra-tumor injections of chondroitinase ABC leads to the development of secondary tumors and increased lung metastases, while primary orthotopic tumor growth was not affected. These results establish a metastasis-inhibiting effect of primary breast tumor-associated chondroitin sulfate, which may open novel carbohydrate-based therapeutic strategies to combat breast cancer. PMID: 22183949 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524541 Thu, 08 Dec 2011 05:00:00 +0100 5524541 http://www.medworm.com/index.php?rid=5524555&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180069%26dopt%3DAbstract Genetic diversity analysis of an indigenous Chinese buffalo breed and hybrids based on microsatellite data. Genet Mol Res. 2011 Dec 5;10(4) Authors: Yang WC, Tang KQ, Mei J, Zeng WB, Yang LG Abstract Chinese native buffaloes have faced the threat of extinction, along with an increase in crossbreeding with domesticated river buffaloes; consequently, conservation of local buffalo genetic resources has become a priority. A Chinese native breed, Jianghan, is often crossed intentionally and unintentionally with imported breeds from India and Pakistan, Murrah, and Nili-Ravi. A total of 128 buffaloes of the breeds Jianghan, Murrah, and Nili-Ravi and their presumed hybrid offspring were genotyped for 10 microsatellite markers. Heterozygosity and Wright's F-statistics were calculate... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524555 Mon, 05 Dec 2011 05:00:00 +0100 5524555 http://www.medworm.com/index.php?rid=5524554&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180070%26dopt%3DAbstract Assessing wheat (Triticum aestivum) genotypes for "Yr" resistance genes using conserved regions and simple-sequence motifs. Genet Mol Res. 2011 Dec 5;10(4) Authors: Cabuk E, Aydin Y, Uncuoglu AA Abstract Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D, to investigate DNA sequence differences and repeat motifs and numbers between wheat cultivars resistant (Izgi2001, Sonmez2001, PI178383) and susceptible (Aytin98, ES14, Harmankaya99) to yellow rust. The F(2) individuals derived from the cros... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524554 Mon, 05 Dec 2011 05:00:00 +0100 5524554 http://www.medworm.com/index.php?rid=5524553&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180071%26dopt%3DAbstract Association of CYP2C19*3 gene polymorphism with breast cancer in Chinese women. Genet Mol Res. 2011 Dec 5;10(4) Authors: Gan CQ, Wang XY, Cao YD, Ye WX, Liu H, Sun YY Abstract Cytochrome P450 (CYP) 2C19 metabolizes arachidonic acid to biologically active epoxyeicosatrienoic acids, which significantly promote proliferation of cancer cells in vitro and in vivo. We looked for a possible association between human CYP2C19*3 gene polymorphism and breast cancer in the Chinese Han population. In a Chinese Han case-control study of breast cancer patients (N = 600) and age- and gender-matched healthy controls (N = 600), we investigated polymorphism in the CYP2C19 gene by PCR-RFLP analysis. The CYP2C19*3 AG + AA genotype was significantly more prevalent in breast cancer patients than ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524553 Mon, 05 Dec 2011 05:00:00 +0100 5524553 http://www.medworm.com/index.php?rid=5524552&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180072%26dopt%3DAbstract We examined 213 diabetic patients, who were divided into retinopathy or no retinopathy groups. Nine selected single nucleotide polymorphisms (SNPs) in the RXR-gamma gene were evaluated. The diabetic retinopathy group had longer diabetes duration, higher body mass indexes, and higher systolic blood pressure, as well as higher concentrations of fasting plasma glucose, blood urine nitrogen, and creatine. One SNP - rs3818569 of the RXR-gamma gene was found to be associated with increased risk for diabetic retinopathy in both allele and genotype frequencies (P = 0.0023 and 0.0057, respectively). Analysis with multivariate logistic regression revealed that the dominant RXR-gamma GG genotype is a risk factors for the development of diabetic retinopathy (odds ratio = 2.388; 95% confidence interval... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524552 Mon, 05 Dec 2011 05:00:00 +0100 5524552 http://www.medworm.com/index.php?rid=5524551&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180073%26dopt%3DAbstract Combining P values to improve classification of differential gene expression in the HTself software. Genet Mol Res. 2011 Dec 5;10(4) Authors: Cortez DA, Tonon AP, Colepicolo P, Vêncio RZ Abstract HTself is a web-based bioinformatics tool designed to deal with the classification of differential gene expression in low replication microarray studies. It is based on a statistical test that uses self-self experiments to derive intensity-dependent cutoffs. We developed an extension of HTself, originally released in 2005, by calculating P values instead of using a fixed acceptance level α. As before, the statistic used to compute single-spot P values is obtained from the Gaussian kernel density estimator method applied to self-self data. Different spots corresponding to the same... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524551 Mon, 05 Dec 2011 05:00:00 +0100 5524551 http://www.medworm.com/index.php?rid=5524550&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180074%26dopt%3DAbstract Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China. Genet Mol Res. 2011 Dec 5;10(4) Authors: Xu HP, He XM, Fang MX, Hu YS, Jia XZ, Nie QH, Zhang XQ Abstract The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and investigate its expression; 16 individuals from four pig breeds (Yorkshire, Landrace, small-ear spotted, and Xinchang) were used to scan the variations. The two transcripts (DRD2L and DRD2S), obtai... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524550 Mon, 05 Dec 2011 05:00:00 +0100 5524550 http://www.medworm.com/index.php?rid=5524549&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180075%26dopt%3DAbstract Molecular sexing of unusually large numbers of Spheniscus magellanicus (Spheniscidae) washed ashore along the Brazilian coast in 2008. Genet Mol Res. 2011 Dec 5;10(4) Authors: Reis EC, Aires RM, Moura JF, Matias CA, Tavares M, Ott PH, Siciliano S, Lôbo-Hajdu G Abstract There have been few studies on Magellanic penguins (Spheniscus magellanicus). In 2008, these penguins washed ashore along the Brazilian coast in unusually high numbers, some reaching as far as northeast Brazil. As Magellanic penguins show little sexual dimorphism, sex determination by morphological features is not accurate. Here, we tested a molecular procedure for sexing specimens of S. magellanicus washed ashore along the coasts of Sergipe, Rio de Janeiro and Rio Grande do Sul in 2008, comparing the sex ra... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524549 Mon, 05 Dec 2011 05:00:00 +0100 5524549 http://www.medworm.com/index.php?rid=5524548&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180076%26dopt%3DAbstract Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP. Genet Mol Res. 2011 Dec 5;10(4) Authors: Ji RQ, Song Q, Xin XF, Zhou X, Feng H Abstract To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-type line, AB01. Sixteen high-quality sequences were generated, 11 of which were up-regulated in fertile buds, and five of which were up-regulated in sterile buds. Based on BLAST screening and functional annotation, these genes have homology... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524548 Mon, 05 Dec 2011 05:00:00 +0100 5524548 http://www.medworm.com/index.php?rid=5524547&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183943%26dopt%3DAbstract Interaction between CYP 2C19*3 polymorphism and smoking in relation to laryngeal carcinoma in the Chinese Han population. Genet Mol Res. 2011 Dec 5;10(4) Authors: Feng J, Li L, Zhao YS, Tang SQ, Yang HB, Liu SX Abstract Cytochrome P450 (CYP) 2C19 metabolizes arachidonic acid to biologically active epoxyeicosatrienoic acids, which strongly promote proliferation of cancer cells in vitro and in vivo. Knowing that smoking is the most important risk factor for laryngeal carcinoma, we examined the relationships between CYP2C19*3 polymorphism, smoking and laryngeal carcinoma in the Chinese Han population. In a Chinese Han case-control study of 300 laryngeal carcinoma patients and 300 healthy controls, we investigated polymorphism in the CYP2C19 gene by PCR-RFLP analysis. The CYP2C... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524547 Mon, 05 Dec 2011 05:00:00 +0100 5524547 http://www.medworm.com/index.php?rid=5524557&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180067%26dopt%3DAbstract We examined the phylogenetic relationships of two carotenoid cleavage dioxygenase genes (CCD7 and CCD8) that are involved in consecutive upstream steps of the proposed strigolactone biosynthesis pathway. The CCD7 and CCD8 sequences from 11 model species, divided into two clades, correspond to sequences from monocotyledons and dicotyledons. However, the sequences from the primitive moss, Physcomitrella patens, appeared to be evolutionarily distinct from those of the angiosperms. CCD7 and CCD8 are much conserved, since no significant positive selection was detected among these plants. Ks values indicated that CCD7 and CCD8 diverged about 290 to 430 million years ago. As essential genes in the strigolactone pathway, the divergence timing of the conserved CCD7 and CCD8 genes reflects the appro... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524557 Fri, 02 Dec 2011 05:00:00 +0100 5524557 http://www.medworm.com/index.php?rid=5524556&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180068%26dopt%3DAbstract Distribution of HLA-B27 and CYP2D6*4 mutations in the middle Black Sea area (Tokat) of Turkey. Genet Mol Res. 2011 Dec 2;10(4) Authors: Sahin S, Aydogan L, Benli I, Ozyurt H Abstract We analyzed distribution of HLA-B27 and CYP2D6*4 mutations in 249 patients from Tokat province in Turkey with symptoms of arthritis, sacroiliac, joint and back pain, using a LightCycler 480 II Real-Time PCR thermal cycler. The Genes-4U was applied for studying HLA-B27 mutation, and the Tib-Molbiol commercial kit was used to examine the CYP2D6*4 mutation. Among the 249 patients, 18.5% had the HLA-B27 mutation. The CYP2D6*4 mutation was found in 22.0% (six homozygotes). Ten patients had both mutations. These frequencies are similar to what has been reported from other populations. PMID: 22180... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524556 Fri, 02 Dec 2011 05:00:00 +0100 5524556 http://www.medworm.com/index.php?rid=5524559&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180065%26dopt%3DAbstract A new approach for cloning hLIF cDNA from genomic DNA isolated from the oral mucous membrane. Genet Mol Res. 2011 Nov 25;10(4) Authors: Cui YH, Zhu GQ, Chen QJ, Wang YF, Yang MM, Song YX, Wang JG, Cao BY Abstract Complementary DNA (cDNA) is valuable for investigating protein structure and function in the study of life science, but it is difficult to obtain by traditional reverse transcription. We employed a novel strategy to clone human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA, which was directly isolated from the mucous membrane of mouth. The hLIF sequence, which is 609 bp long and is composed of three exons, can be acquired within a few hours by amplifying each exon and splicing all of them using overlap-PCR. This new approach developed is simple, time... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524559 Fri, 25 Nov 2011 05:00:00 +0100 5524559 http://www.medworm.com/index.php?rid=5524558&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180066%26dopt%3DAbstract The role of α-satellite DNA and heterochromatin polymorphism in leukemia patients and illicit drug addicts. Genet Mol Res. 2011 Nov 25;10(4) Authors: Movafagh A, Mortazavi-Tabatabaei SA, Kolahi AA Abstract Heterochromatin is considered to play a role in protecting the genome against mutagens. Changes in the quantity and proportion of different types of satellite DNA could increase genetic susceptibility in individuals with heterochromatic variations; they cause chromosome instability and predispose patients to malignancies. We evaluated the heterochromatin associated with chromosomes in 50 leukemia patients, 93 drug addicts and 93 healthy controls from Tehran, Iran. Barium hydroxide saline Giemsa staining was used to examine heterochromatin polymorphism of chromosomes 1, 9... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5524558 Fri, 25 Nov 2011 05:00:00 +0100 5524558 http://www.medworm.com/index.php?rid=5435868&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095595%26dopt%3DAbstract We examined the effects of environmental and genetic factors on the weaning-to-estrus interval (WEI) in sows. In order to perform the analyses of the environmental factors, 8104 observations of the 1st to the 6th WEI were carried out, while 6548 observations of the 1st to the 3rd WEI were carried out for the analyses of genetic factors. The environmental model included as fixed effects, herd, genetic line, year and season of birth, as well as the covariates, age of sow at farrowing, litter size at birth and lactation length. Genetic analysis was performed by repeatability and multitrait models. The mean and coefficient of variation for WEI were 7.02 days and 100.6%, respectively. The linear effect of lactation length and the quadratic effect of the age of sow at farrowing affected the WEI.... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435868 Wed, 23 Nov 2011 01:36:03 +0100 5435868 http://www.medworm.com/index.php?rid=5435867&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095596%26dopt%3DAbstract Lactic acid bacteria dynamics during spontaneous fermentation of cocoa beans verified by culture-independent denaturing gradient gel electrophoresis. Genet Mol Res. 2011;10(4):2702-9 Authors: Santos TF, Santana LK, Santos AC, Silva GS, Romano CC, Dias JC, Rezende RP Abstract Cocoa is naturally fermented in the field before the cocoa seeds are removed for processing. We assessed the dynamics of lactic acid bacteria during cocoa fermentation in Bahia, Brazil. During five days of fermentation, temperature and pH were measured and beans were collected for genomic DNA extraction every 12 h. The DNA was used as a template for amplification with Lac1-Lac2 and Lac3-Lac2 for denaturing gradient gel electrophoresis analyses. pH values ranged from 3.34 to 4.98, while the temperature v... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435867 Wed, 23 Nov 2011 01:36:03 +0100 5435867 http://www.medworm.com/index.php?rid=5435866&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095597%26dopt%3DAbstract This study reports on a cytogenetic finding in a bone marrow examination of a 47-year-old male patient treated in the Hematology and Blood Transfusion Service of the Hospital de Base in São José do Rio Preto, São Paulo State, Brazil. The only alteration found at diagnosis of myelodysplastic syndrome (MDS) subtype refractory anemia with excess blasts (RAEB-2) was clonal monosomy of chromosome 21. The patient evolved to acute myeloid leukemia type M2 and died nine months after diagnosis. Clonal monosomy of chromosome 21, as the only cytogenetic abnormality in MDS, has only been reported three times previously. This uncommon cytogenetic abnormality in MDS has been associated with a poor clinical course, although more data will be needed to determine if this prognosis is invariable. PMI... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435866 Wed, 23 Nov 2011 01:36:03 +0100 5435866 http://www.medworm.com/index.php?rid=5435865&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095598%26dopt%3DAbstract A novel polymorphism of the myogenin gene is associated with body measurement traits in native Chinese breeds. Genet Mol Res. 2011;10(4):2721-8 Authors: Xue M, Zan LS, Gao L, Wang HB Abstract Using PCR-SSCP and DNA sequencing technology, we examined the association of single nucleotide polymorphisms (SNPs) in the bovine MyoG gene with body measurement traits in 779 individuals of six native Chinese cattle breeds, namely Luxi, Luxi × Simmental crossbred, Nanyang, Xia'nan, Jiaxian red, and Qinchuan. A novel SNP, T314C, was detected. Allelic frequencies of MyoG-T/C in the six breeds were 0.8308/0.1692, 0.8774/0.1226, 0.8021/0.1979, 0.8209/0.1791, 0.8630/0.1370, 0.8044/0.1956, respectively. Least squares analysis revealed a significant (P < 0.05) association of the MyoG SNP... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435865 Wed, 23 Nov 2011 01:36:03 +0100 5435865 http://www.medworm.com/index.php?rid=5435864&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095599%26dopt%3DAbstract Assessing molecular and morpho-agronomical diversity and identification of ISSR markers associated with fruit traits in quince (Cydonia oblonga). Genet Mol Res. 2011;10(4):2729-46 Authors: Ganopoulos I, Merkouropoulos G, Pantazis S, Tsipouridis C, Tsaftaris A Abstract Quince is a deciduous tree known to the countries around the Mediterranean since antiquity. Nowadays, quince is used as an ornamental plant, and as a rootstock for pear trees, with its fruit being appreciated mainly for production of jam and sweets rather than for raw consumption. Quince leaves contain compounds with antioxidant, antimicrobial and anticancerous properties that have been the focus of recent research on pharmaceutical and medical uses as well as for food preservatives. An orchard has been establ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435864 Wed, 23 Nov 2011 01:36:03 +0100 5435864 http://www.medworm.com/index.php?rid=5435863&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095600%26dopt%3DAbstract AGPAT6 polymorphism and its association with milk traits of dairy goats. Genet Mol Res. 2011;10(4):2747-56 Authors: He C, Wang C, Chang ZH, Guo BL, Li R, Yue XP, Lan XY, Chen H, Lei CZ Abstract As one of the eight members in the 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs) family, AGPAT6 is a crucial enzyme for the biosynthesis of glycerolipids and triacylglycerol in eukaryotes, as well as catalyzing the conversion from lysophosphatidic acid to phosphatidic acid. AGPAT6 can be considered as a candidate gene for regulating milk composition. DNA sequencing and PCR-RFLP methods were applied to detect genetic variation in the AGPAT6 gene in 549 Chinese dairy goats. Four polymorphisms (NC_007328.3:g.152G>C, 8124G>A, 9263C>G, 16436G>A) were detected in 5'UTR,... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435863 Wed, 23 Nov 2011 01:36:03 +0100 5435863 http://www.medworm.com/index.php?rid=5435862&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095601%26dopt%3DAbstract Authors: Mohd-Hairul AR, Sade AB, Yiap BC, Raha AR Abstract DNA extraction was carried out on 32 medicinal plant samples available in Malaysia using the TriOmic(TM) extraction kit. Amounts of 0.1 g flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1(plus) buffer and followed by RNAse treatment. Then, RY2 buffer was added to the samples and mixed completely by vortexing before removal of cell debris by centrifugation. Supernatants were transferred to fresh microcentrifuge tubes and 0.1 volume RY3 buffer was added to each of the transferred supernatant. The mixtures were applied to spin columns followed by a centrifugation step to remove buffers and other residues. Washing step was carried out twice by applying 70% ethanol to the spin columns. Genomic DNA ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435862 Wed, 23 Nov 2011 01:36:03 +0100 5435862 http://www.medworm.com/index.php?rid=5435861&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095602%26dopt%3DAbstract In conclusion, we found no association between TNF-α -308G>A polymorphism and liver cirrhosis risk, both in Caucasian and Asian populations. PMID: 22095602 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435861 Wed, 23 Nov 2011 01:36:03 +0100 5435861 http://www.medworm.com/index.php?rid=5435860&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095603%26dopt%3DAbstract Population structure and demographic inferences concerning the endangered onychophoran species Epiperipatus acacioi (Onychophora: Peripatidae). Genet Mol Res. 2011;10(4):2775-85 Authors: Lacorte GA, Oliveira IS, Fonseca CG Abstract Epiperipatus acacioi (Onychophora: Peripatidae) is an endemic species of the Atlantic rainforest in southeastern Brazil, with a restricted known distribution, found only in two nearby areas (Tripuí and Itacolomi). Mitochondrial gene COI sequences of 93 specimens collected across the known range of E. acacioi were used to assess the extant genetic diversity and patterns of genetic structure, as well as to infer the demographic history of this species. We found considerable variability within the populations, even though there has been recent envi... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435860 Wed, 23 Nov 2011 01:36:03 +0100 5435860 http://www.medworm.com/index.php?rid=5435859&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095604%26dopt%3DAbstract Molecular differentiation of species of the genus Zungaro (Siluriformes, Pimelodidae) from the Amazon and Paraná-Paraguay River basins in Brazil. Genet Mol Res. 2011;10(4):2795-805 Authors: Boni TA, Padial AA, Prioli SM, Lucio LC, Maniglia TC, Bignotto TS, Panarari-Antunes RS, Prioli RA, Prioli AJ Abstract Fish species of the Zungaro genus (Siluriformes, Pimelodidae) are amongst the largest migratory fish in Latin America and have considerable economic importance for commercial fishing in Brazil. However, natural populations of this large catfish are experiencing a severe decline. There are significant taxonomical inconsistencies for this fish. Two geographically separated species of the fish were initially described, one endemic in the Amazon and another in the Paraná-Pa... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435859 Wed, 23 Nov 2011 01:36:03 +0100 5435859 http://www.medworm.com/index.php?rid=5435858&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095605%26dopt%3DAbstract Molecular authentication of the medicinal herb Ruta graveolens (Rutaceae) and an adulterant using nuclear and chloroplast DNA markers. Genet Mol Res. 2011;10(4):2806-16 Authors: Al-Qurainy F, Khan S, Tarroum M, Al-Hemaid FM, Ali MA Abstract Dried parts of different plant species often look alike, especially in powdered form, making them very difficult to identify. Ruta graveolens, sold as a dried medicinal herb, can be adulterated with Euphorbia dracunculoides. The genomic DNA was isolated from the leaf powder (100 mg each) using the modified CTAB method. Internal transcribed spacer sequences of nuclear ribosomal DNA (nrDNA-ITS), and chloroplast spacer sequences (rpoB and rpoC1) are regarded as potential genes for plant DNA barcoding. We amplified and sequenced these space... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435858 Wed, 23 Nov 2011 01:36:03 +0100 5435858 http://www.medworm.com/index.php?rid=5435857&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095606%26dopt%3DAbstract We examined the expression of at least one miRNA member from each miRNA family in rice roots of Oryza sativa spp indica cv. Embrapa Taim and Oryza sativa spp japonica cv. Nipponbare under high concentrations of aluminum. Forty-six miRNA families were effectively detected by quantitative PCR. Among these, 13 were down-regulated and six were up-regulated in roots of the Nipponbare cultivar after 8 h of aluminum treatment. In roots of the Embrapa Taim cultivar, five miRNAs were down-regulated and three were up-regulated. Analyses of their putative targets suggest that these rice miRNAs are involved in the regulation of various metabolic pathways in response to high concentrations of aluminum. PMID: 22095606 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435857 Wed, 23 Nov 2011 01:36:03 +0100 5435857 http://www.medworm.com/index.php?rid=5435856&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095607%26dopt%3DAbstract Association between a single nucleotide polymorphism in the bovine chemerin gene and carcass traits in Qinchuan cattle. Genet Mol Res. 2011;10(4):2833-40 Authors: Tian WQ, Wang HC, Song FB, Zan LS, Wang H, Wang HB, Xin YP, Ujan JA Abstract Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was detected in 716 samples of six breeds (Jiaxian red, Luxi, Nan yang, Qinchuan, Simmental and Luxi crossbred steers, and Xia'nan), all in China, and three genotypes... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435856 Wed, 23 Nov 2011 01:36:03 +0100 5435856 http://www.medworm.com/index.php?rid=5435855&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095608%26dopt%3DAbstract Lack of association between RANTES-28, SDF-1 gene polymorphisms and systemic lupus erythematosus in the Malaysian population. Genet Mol Res. 2011;10(4):2841-50 Authors: Lian LH, Kee BP, Ng HL, Chua KH Abstract Regulated on activation, normal T-cell expressed and secreted (RANTES) and stromal cell-derived factor 1 (SDF-1) are members of the CC- and CXC-chemokine families, respectively. Both genes have been postulated to be involved in the pathogenesis of systemic lupus erythematosus (SLE). We analyzed position 28 of the RANTES gene promoter region, as well as the SNP observed in the 3' UTR of the SDF-1 gene at position 801, in 130 patients presenting SLE at the Malaya University Medical Centre. Screening of 130 healthy volunteer controls using RFLP was also performed. RANTES... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435855 Wed, 23 Nov 2011 01:36:03 +0100 5435855 http://www.medworm.com/index.php?rid=5435854&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095609%26dopt%3DAbstract Multiple displacement amplification for preimplantation genetic diagnosis of fragile X syndrome. Genet Mol Res. 2011;10(4):2851-9 Authors: Lee HS, Kim MJ, Lim CK, Cho JW, Song IO, Kang IS Abstract Preimplantation genetic diagnosis (PGD) has become an assisted reproductive technique for couples that have genetic risks. Despite the many advantages provided by PGD, there are several problems, including amplification failure, allele drop-out and amplification inefficiency. We evaluated multiple displacement amplification (MDA) for PGD of the fragile X syndrome. Whole genome amplification was performed using MDA. MDA products were subjected to fluorescent PCR of fragile X mental retardation-1 (FMR1) CGG repeats, amelogenin and two polymorphic markers. In the pre-clinical tests, ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435854 Wed, 23 Nov 2011 01:36:03 +0100 5435854 http://www.medworm.com/index.php?rid=5435871&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095481%26dopt%3DAbstract Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues. Genet Mol Res. 2011 Nov 17;10(4) Authors: Pinheiro TT, Litholdo Jr CG, Sereno ML, Leal Jr GA, Albuquerque PS, Figueira A Abstract Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for gene expression analysis, but relative expression analysis requires various reference genes, which must be stable across various biological conditions. We sought suitable reference genes for various tissues of cacao, especially developing seeds. Ten potential reference genes were analyzed f... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435871 Thu, 17 Nov 2011 05:00:00 +0100 5435871 http://www.medworm.com/index.php?rid=5435870&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095482%26dopt%3DAbstract High degree of genetic diversity among genotypes of the forage grass Brachiaria ruziziensis (Poaceae) detected with ISSR markers. Genet Mol Res. 2011 Nov 17;10(4) Authors: Azevedo AL, Costa PP, Machado MA, de Paula CM, Sobrinho FS Abstract The grasses of the genus Brachiaria account for 80% of the cultivated pastures in Brazil. Despite its importance for livestock production, little information is available for breeding purposes. Embrapa has a population of B. ruziziensis from different regions of Brazil, representing most of existing variability. This population was used to initiate an improvement program based on recurrent selection. In order to assist the genetic improvement program, we estimated the molecular variability among 93 genotypes of Embrapa's collection using ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435870 Thu, 17 Nov 2011 05:00:00 +0100 5435870 http://www.medworm.com/index.php?rid=5435869&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22095483%26dopt%3DAbstract Successful preimplantation genetic aneuploidy screening in Turkish patients. Genet Mol Res. 2011 Nov 17;10(4) Authors: Ercelen N, Turtar E, Gultomruk M, Comert H, Coskun H, Mercan R, Nuhoglu A Abstract Preimplantation genetic diagnosis is a preventive approach for identifying genetic abnormalities in early stages of reproduction. We used preimplantation genetic aneuploidy screening in 230 cycles of patients with indications of advanced maternal age, recurrent implantation failure, recurrent spontaneous abortions, or severe male factor. Biopsied blastomeres from embryos with six to eight blastomeres on day 3 were fixed and fluorescence in situ hybridization was utilized on chromosomes 13, 16, 18, 21, 22, X, and Y. Among 945 morphologically normal embryos, 314 were diagnosed ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5435869 Thu, 17 Nov 2011 05:00:00 +0100 5435869 http://www.medworm.com/index.php?rid=5410488&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057955%26dopt%3DAbstract We examined the effect of incorporation of molecular markers on variability between and within populations in order to maximize heterotic effects and longevity of a maize reciprocal recurrent selection program. Molecular variability was quantified by inter-simple sequence repeat (ISSR) markers between and within the maize populations Cimmyt and Piranão in the 10th cycle of a reciprocal recurrent selection program. Forty-two S(1) progenies of each population were analyzed, these being families of full-sibs selected according to their agronomic traits. Thirteen primers were selected, which produced 140 bands; 114 of them were polymorphic and 26 monomorphic. Based on UPGMA grouping analysis and by genetic distances, it was possible to identify "contaminant" progenies. These progenies belong ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410488 Thu, 17 Nov 2011 04:36:02 +0100 5410488 http://www.medworm.com/index.php?rid=5410487&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057956%26dopt%3DAbstract In conclusion, MTRR A66G and C524T polymorphisms are associated with increased risk of CHDs. PMID: 22057956 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410487 Thu, 17 Nov 2011 04:36:02 +0100 5410487 http://www.medworm.com/index.php?rid=5410486&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057957%26dopt%3DAbstract Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F(1) generation. Genet Mol Res. 2011;10(4):2606-12 Authors: Matoso DA, da Silva M, Cortinhas MC, Cestari MM, de Almeida MC, Vicari MR, Artoni RF Abstract Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F(1) fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens. The results suggest different genetic lineages in sympatry in nature. Heterozygosity and percentage of polymorphic loci were 0.3... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410486 Thu, 17 Nov 2011 04:36:02 +0100 5410486 http://www.medworm.com/index.php?rid=5410485&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057958%26dopt%3DAbstract Genome-wide identification, phylogeny and expression analysis of the lipoxygenase gene family in cucumber. Genet Mol Res. 2011;10(4):2613-36 Authors: Liu SQ, Liu XH, Jiang LW Abstract Plant lipoxygenase (LOX) is involved in growth and developmental control processes, through the biosynthesis of regulatory molecules and defense responses to pathogens, wounding and stress. To date, few LOX proteins and little tissue expression profiling have been reported in detail for cucumber (Cucumis sativus L.). Recent completion of the cucumber genome sequence now permits genome-wide analysis of the LOX gene family in cucumber as well as comparison with LOX in Arabidopsis and rice. We identified 23 candidate LOX genes in the cucumber genome; phylogenetic analysis indicated that these LOX... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410485 Thu, 17 Nov 2011 04:36:02 +0100 5410485 http://www.medworm.com/index.php?rid=5410484&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057959%26dopt%3DAbstract Functional characterization and signal transduction ability of nucleotide-binding site-leucine-rich repeat resistance genes in plants. Genet Mol Res. 2011;10(4):2637-52 Authors: Joshi RK, Nayak S Abstract Pathogen infection in plants is often limited by a multifaceted defense response triggered by resistance genes. The most prevalent class of resistance proteins includes those that contain a nucleotide-binding site-leucine-rich repeat (NBS-LRR) domain. Over the past 15 years, more than 50 novel NBS-LRR class resistance genes have been isolated and characterized; they play a significant role in activating conserved defense-signaling networks. Recent molecular research on NBS-LRR resistance proteins and their signaling networks has the potential to broaden the use of resistan... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410484 Thu, 17 Nov 2011 04:36:02 +0100 5410484 http://www.medworm.com/index.php?rid=5410483&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057960%26dopt%3DAbstract We examined 40 female blood samples to verify the specificity of RHD exons (7 and 10) amplified by real-time PCR. Thirty fetuses were predicted to be RHD-positive based on analysis of plasma DNA. Seven fetuses were predicted to be RHD-negative. One fetus was negative for RHD on exon 10, and positive for RHD on exon 7 (early gestation age); two fetuses were RHD-negative on exon 7, and RHD-positive on exon 10 (RHD-CE-D(s) or RHDΨ), indicative of a maternal RHD allele. We conclude that it is necessary to analyze at least two exon regions in the RHD gene. PMID: 22057960 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410483 Thu, 17 Nov 2011 04:36:02 +0100 5410483 http://www.medworm.com/index.php?rid=5410482&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057961%26dopt%3DAbstract Using PCR for early diagnosis of bovine leukemia virus infection in some native cattle. Genet Mol Res. 2011;10(4):2658-63 Authors: Mohammadabadi MR, Soflaei M, Mostafavi H, Honarmand M Abstract Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results obtained by immunodiffusion or ELISA. The native Iranian and Russian cattle have a series of valuable traits that discriminate them as unique breeds that are well able to compete with western analogues. Ho... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410482 Thu, 17 Nov 2011 04:36:02 +0100 5410482 http://www.medworm.com/index.php?rid=5410481&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057962%26dopt%3DAbstract Smith-Magenis syndrome: clinical evaluation in seven Brazilian patients. Genet Mol Res. 2011;10(4):2664-70 Authors: Gamba BF, Vieira GH, Souza DH, Monteiro FF, Lorenzini JJ, Carvalho DR, Morreti-Ferreira D Abstract Smith-Magenis syndrome (SMS) is a complex congenital anomaly characterized by craniofacial anomalies, neurological and behavioral disorders. SMS is caused by a deletion in region 17p11.2, which includes the RAI1 gene (90% of cases), or by point mutation in the RAI1 gene (10% of cases). Laboratory diagnosis is through cytogenetic analysis by GTG banding and molecular cytogenetic analysis by FISH. We carried out an active search for patients in Associations of Parents and Friends of Exceptional Children (APAE) of São Paulo and genetic centers in Brazil. Forty-eigh... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410481 Thu, 17 Nov 2011 04:36:02 +0100 5410481 http://www.medworm.com/index.php?rid=5410480&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057963%26dopt%3DAbstract In conclusion, a synthetic G-CSF cDNA in combination with the recombinant DNA protocol offers a rapid and reliable strategy for synthesizing the target protein. However, commercial utilization of this methodology will require rigorous validation and quality control. PMID: 22057963 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410480 Thu, 17 Nov 2011 04:36:02 +0100 5410480 http://www.medworm.com/index.php?rid=5410479&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22057964%26dopt%3DAbstract We examined the diversity of the gut microbiota of S. levis, which was characterized using culture-dependent and culture-independent methods. Analysis of 16S rRNA amplified directly from the gut community revealed the presence of 14 genera, one group from the Candidatus category, one uncultured group assigned to the family Flavobacteriaceae, and one uncultured group assigned to the family Enterobacteriaceae; all of them are members of the Alpha-Proteobacteria, Beta-Proteobacteria, Gamma-Proteobacteria, Firmicutes, and Bacteroidetes phyla. Microorganisms isolated through culture-dependent methods were classified according to morphological parameters and by 16S rRNA gene sequences. In addition to bacteria, four filamentous fungi were isolated. A higher bacterial diversity was observed in fie... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5410479 Thu, 17 Nov 2011 04:36:02 +0100 5410479 http://www.medworm.com/index.php?rid=5375827&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21491373%26dopt%3DAbstract Authors: Estevão IF, Peitl Junior P, Bonini-Domingos CR Abstract There have been few studies on the mutations that cause heterozygous beta-thalassemia and how they affect the iron profile. One hundred and thirty-eight individuals were analyzed, 90 thalasemic β⁰ and 48 thalasemic β(+), identified by classical and molecular methods. Mutations in the hemochromatosis (HFE) gene, detected using PCR-RFLP, were found in 30.4% of these beta-thalassemic patients; heterozygosity for H63D (20.3%) was the most frequent. Ferritin levels and transferrin saturation were similar in beta-thalassemics with and without mutations in the HFE gene. Ferritin concentrations were significantly higher in men and in individuals over 40 years of age. Transferrin saturation also was significantly higher i... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375827 Fri, 04 Nov 2011 22:25:04 +0100 5375827 http://www.medworm.com/index.php?rid=5375826&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002122%26dopt%3DAbstract Polymorphisms associated with egg number at 300 days of age in chickens. Genet Mol Res. 2011;10(4):2279-89 Authors: Xu HP, Zeng H, Zhang DX, Jia XL, Luo CL, Fang MX, Nie QH, Zhang XQ Abstract We looked for variations that could be associated with chicken egg number at 300 days of age (EN300) in seven genes of the hypothalamic-pituitary-gonadal axis, including gonadotrophin-releasing hormone-I (GnRH-I), GnRH receptor (GnRHR), neuropeptide Y (NPY), dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP), VIP receptor-1 (VIPR-1), prolactin (PRL), and the QTL region between 87 and 105 cM of the Z chromosome. Ten mutations in the seven genes were chosen to do marker-trait association analyses in a population comprising 1310 chickens, which were obtained from a compa... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375826 Fri, 04 Nov 2011 22:25:04 +0100 5375826 http://www.medworm.com/index.php?rid=5375825&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002123%26dopt%3DAbstract Identification and expression analysis of the Broad-Complex core protein isoform 6 (BR-C Z6) gene in the giant tiger shrimp Penaeus monodon (Penaeidae: Decapoda). Genet Mol Res. 2011;10(4):2290-306 Authors: Buaklin A, Klinbunga S, Mensveta P Abstract Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to polypeptides of 479 and 480 amino acids, respectively. Tissue distribution analysis indicated that PmBR-C Z6 was abundantly expressed in hemocytes and ovaries in juveniles. I... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375825 Fri, 04 Nov 2011 22:25:04 +0100 5375825 http://www.medworm.com/index.php?rid=5375824&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002124%26dopt%3DAbstract Prediction of genomic islands in seven human pathogens using the Z-Island method. Genet Mol Res. 2011;10(4):2307-15 Authors: Wei W, Guo FB Abstract We adopted the method of Zhang and Zhang (the Z-Island method) to identify genomic islands in seven human pathogens, analyzing their chromosomal DNA sequences. The Z-Island method is a theoretical method for predicting genomic islands in bacterial genomes; it consists of determination of the cumulative GC profile and computation of codon usage bias. Thirty-one genomic islands were found in seven pathogens using this method. Further analysis demonstrated that most have the known conserved features; this increases the probability that they are real genomic islands. Eleven genomic islands were found to code for products involved in... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375824 Fri, 04 Nov 2011 22:25:04 +0100 5375824 http://www.medworm.com/index.php?rid=5375823&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002125%26dopt%3DAbstract Loss of heterozygosity on chromosome 5 in Iranian esophageal cancer patients. Genet Mol Res. 2011;10(4):2316-25 Authors: Attaran-Bandarabadi F, Ziaee AA, Yazdanbod M, Shahpanah M, Setayeshgar A, Nassiri M Abstract There is a high incidence of esophageal squamous cell carcinoma (ESCC) in Iran. Non-functionality of some tumor suppressor genes has been reported in esophageal cancer. Loss of heterozygosity on chromosome 5 has also been reported in esophageal carcinomas. We assessed loss of heterozygosity along a region of the long arm of chromosome 5 (5q), from 5q23.1 to 5q23.2, by PCR amplifying DNA fragments of tumor tissues from patients with ESCC and their corresponding normal samples. The PCR products were electrophoresed on 6% non-denaturing polyacrylamide gels, and band ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375823 Fri, 04 Nov 2011 22:25:04 +0100 5375823 http://www.medworm.com/index.php?rid=5375822&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002126%26dopt%3DAbstract Authors: Li JJ, Liu L, Ouyang YD, Yao JL Abstract Apomixis is a particular mode of reproduction that allows progeny formation without meiosis and fertilization. Eulaliopsis binata, a tetraploid apomictic species, is widely used for making paper, rope and mats. There is great potential for fixation of heterosis in E. binata due to autonomous endosperm formation in this species. Although most of its embryo sac originates from nucellus cells, termed apospory, we observed sexual reproduction initiation in 86.8 to 96.8% of the ovules, evidenced by callose deposition on the walls of cells undergoing megasporogenesis. However, only 2-3% mature polygonum-type sexual embryo sacs were confirmed by embryological investigation. Callose was not detected on aposporous initial cell walls. The apo... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375822 Fri, 04 Nov 2011 22:25:04 +0100 5375822 http://www.medworm.com/index.php?rid=5375821&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002127%26dopt%3DAbstract We examined NDEA genotoxicity and cytotoxicity in primary cultures of female rat hepatocytes; we also looked at how it affected CYP mRNA expression. Single incubation with 0.9% NaCl resulted in a mean of 0.2% apoptotic cells, which doubled with 105 μg NDEA/mL. The frequency of necrosis with NDEA treatment was also doubled. Besides the cytotoxic effects, there was also a 4-fold decrease in mitotic index and a 3-fold decrease in the percentage of cells with micronuclei. A significant increase in micronucleus cells when hepatocytes were incubated with 2.1 μg NDEA/mL suggests that DNA repair was inactive. The chromosomal aberration evaluation revealed a discrete dose-response curve. Treatment with NDEA induced increases in CYP mRNA: CYP2B2 (1.8 times) and CYP2E1 (1.6 times) with non-cytotoxi... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375821 Fri, 04 Nov 2011 22:25:04 +0100 5375821 http://www.medworm.com/index.php?rid=5375820&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002128%26dopt%3DAbstract A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai. Genet Mol Res. 2011;10(4):2349-57 Authors: Peng ZS, Li X, Yang ZJ, Liao ML Abstract Aiganfanmai is a dwarf tetraploid wheat landrace (Triticum turgidum var. turgidum) that stably produces the semi-dwarf trait. Plant height varies from 80-105 cm under cultivation. Compared with tall durum wheat (T. turgidum var. durum) variety Langdon, we found it to have short spikes and seeds, besides a semi-dwarf character. We crossed Aiganfanmai with Langdon to analyze the genetic basis of the semi-dwarf trait. The F(2) population segregated at a 1:3 ratio for the short trait to the normal, which demonstrates that Aiganfanmai carries a recessive reduced height (Rht) gene. This gene was found to be lo... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375820 Fri, 04 Nov 2011 22:25:04 +0100 5375820 http://www.medworm.com/index.php?rid=5375819&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002129%26dopt%3DAbstract Molecular traceability of beef from synthetic Mexican bovine breeds. Genet Mol Res. 2011;10(4):2358-65 Authors: Rodríguez-Ramírez R, Arana A, Alfonso L, González-Córdova AF, Torrescano G, Guerrero Legarreta I, Vallejo-Cordoba B Abstract Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality brands. Molecular meat traceability would allow verification of conventional methods used for beef tracing in synthetic Mexican bovine breeds. We evalua... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375819 Fri, 04 Nov 2011 22:25:04 +0100 5375819 http://www.medworm.com/index.php?rid=5375818&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002130%26dopt%3DAbstract Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. Genet Mol Res. 2011;10(4):2366-81 Authors: Missio RF, Caixeta ET, Zambolim EM, Pena GF, Zambolim L, Dias LA, Sakiyama NS Abstract Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), res... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375818 Fri, 04 Nov 2011 22:25:04 +0100 5375818 http://www.medworm.com/index.php?rid=5375817&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002131%26dopt%3DAbstract Comparison of RAPD and ISSR markers for assessment of genetic diversity among endangered rare Dalbergia oliveri (Fabaceae) genotypes in Vietnam. Genet Mol Res. 2011;10(4):2382-93 Authors: Phong DT, Hien VT, Thanh TT, Tang DV Abstract Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 RAPD and 27 ISSR) were used. The RAPD primers produced 63 bands across 35 genotypes, of which 24 were polymorphic. The number of amplified bands varied from one... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375817 Fri, 04 Nov 2011 22:25:04 +0100 5375817 http://www.medworm.com/index.php?rid=5375816&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002132%26dopt%3DAbstract Genetic analysis of the Venezuelan Criollo horse. Genet Mol Res. 2011;10(4):2394-403 Authors: Cothran EG, Canelon JL, Luis C, Conant E, Juras R Abstract Various horse populations in the Americas have an origin in Spain; they are remnants of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). We evaluated genetic variability within the Venezuelan Criollo horse and its relationship with other horse breeds. We observed high levels of genetic diversity within the Criollo breed. Significant population differentiation was observed between all South American breeds. The Venezuelan Criollo horse showed high levels of genetic diversity, and from a conservation standpoint, there is no immediate danger of losing variation unless ther... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375816 Fri, 04 Nov 2011 22:25:04 +0100 5375816 http://www.medworm.com/index.php?rid=5375815&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002133%26dopt%3DAbstract Introgression of cotton leaf curl virus-resistant genes from Asiatic cotton (Gossypium arboreum) into upland cotton (G. hirsutum). Genet Mol Res. 2011;10(4):2404-14 Authors: Ahmad S, Mahmood K, Hanif M, Nazeer W, Malik W, Qayyum A, Hanif K, Mahmood A, Islam N Abstract Cotton is under the constant threat of leaf curl virus, which is a major constraint for successful production of cotton in the Pakistan. A total of 3338 cotton genotypes belonging to different research stations were screened, but none were found to be resistant against the Burewala strain of cotton leaf curl virus (CLCuV). We explored the possibility of transferring virus-resistant genes from Gossypium arboreum (2n = 26) into G. hirsutum (2n = 52) through conventional breeding techniques. Hybridization was don... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375815 Fri, 04 Nov 2011 22:25:04 +0100 5375815 http://www.medworm.com/index.php?rid=5375814&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002134%26dopt%3DAbstract Analysis of CD38 and ZAP70 mRNA expression among cytogenetic subgroups of Iranian chronic-lymphocytic-leukemia patients. Genet Mol Res. 2011;10(4):2415-23 Authors: Teimori H, Akbari MT, Hamid M, Forouzandeh M, Bibordi E Abstract Chromosomal abnormalities and ZAP70 expression profile are two major independent prognostic markers in B-cell chronic lymphocytic leukemia. We investigated a possible correlation between these two markers. ZAP70 expression using real-time RT-PCR was examined in 20 B-cell chronic lymphocytic leukemia patients with del13q14, 13 patients with del11q22, 15 patients with trisomy 12, and 16 patients with no detected chromosomal abnormalities. Molecular analysis revealed that ZAP70 expression in the del13q subgroup was the same as in the control group, whi... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375814 Fri, 04 Nov 2011 22:25:04 +0100 5375814 http://www.medworm.com/index.php?rid=5375813&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22002135%26dopt%3DAbstract Prevalence of cystathionine beta synthase gene mutation 852Ins68 as a possible risk for neural tube defects in eastern India. Genet Mol Res. 2011;10(4):2424-9 Authors: Saxena AK, Gupta J, Pandey S, Gangopadhaya AN, Pandey LK Abstract Cystathionine beta synthase gene (CβS) catalyzes the condensation of homocysteine with serine, forming cystathionine by the transsulfuration pathway. Disruption of CβS enzyme activity due to defective folic acid metabolism increases the risk factor for neural tube defects. We evaluated the CβS gene mutation in 25 children with neural tube defects (NTDs), including lumbosacral and thoracic myelomeningocele and open NTDs and mothers of cases, along with 25 healthy children and their mothers, serving as controls. Genomic DNA was isolated to ass... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375813 Fri, 04 Nov 2011 22:25:04 +0100 5375813 http://www.medworm.com/index.php?rid=5375812&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009856%26dopt%3DAbstract Identification of complex vertebral malformation carriers in Holstein cattle in south China. Genet Mol Res. 2011;10(4):2443-8 Authors: Wang C, Tong Q, Hu XZ, Yang LG, Zhong XQ, Yu Y, Wu JJ, Liu WJ, Li X, Hua GH, Zhao HQ, Zhang SJ Abstract Complex vertebral malformation (CVM) is a recently described monogenic autosomal recessive hereditary defect of Holstein dairy cattle that causes premature birth, aborted fetuses and stillborn calves. Guanine is substituted by thymine (G>T) in the solute carrier family 35 member A3 gene (SLC35A3). A valine is changed to a phenylalanine at position 180 of uridine 5'-diphosphate-N-acetyl-glucosamine transporter protein. CVM is expected to occur in many countries due to the widespread use of sire semen. We developed a created restriction s... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375812 Fri, 04 Nov 2011 22:25:04 +0100 5375812 http://www.medworm.com/index.php?rid=5375811&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009857%26dopt%3DAbstract Meta-analysis of the relationship between p21 Ser31Arg polymorphism and lung cancer susceptibility. Genet Mol Res. 2011;10(4):2449-56 Authors: Lin G, Fang F, Yu XJ, Yu L Abstract The cyclin-dependent kinase inhibitor 1A (also known as p21) is thought to be involved in tumor development by mediating cell cycle arrest through the inhibition of cyclin/CDK activity. To explore the relationship of Ser31Arg polymorphism in the p21 gene with the risk of developing lung cancer, we performed an overall and stratified meta-analysis based on ethnicity, lung cancer subtypes and source of controls, with six eligible studies (2366 cases and 3320 controls). No significant variation in lung cancer risk was detected in any of the genetic models in the overall, and the ethnicity-based and ca... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375811 Fri, 04 Nov 2011 22:25:04 +0100 5375811 http://www.medworm.com/index.php?rid=5375810&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009858%26dopt%3DAbstract Multivariate analysis of morphological characteristics of two species of passion flower with ornamental potential and of hybrids between them. Genet Mol Res. 2011;10(4):2457-71 Authors: Santos EA, Souza MM, Viana AP, Almeida AA, Freitas JC, Lawinscky PR Abstract We estimated genetic parameters through multivariate analysis of two species of Passiflora and their hybrids, considered of ornamental potential, based on the morphological characteristics: flower diameter, corona diameter, corona filament size, flower peduncle length, petal length and width, sepal length and width, internode length, stem diameter, leaf length, leaf width (mm), and leaf area (cm(2)). Five specimens of Passiflora sublanceolata [ex P. palmeri var. sublanceolata], five of P. foetida var. foetida and 20... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375810 Fri, 04 Nov 2011 22:25:04 +0100 5375810 http://www.medworm.com/index.php?rid=5375809&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009859%26dopt%3DAbstract Esterase polymorphisms for analysis of genetic diversity and structure of soybean (Glycine max) cultivars. Genet Mol Res. 2011;10(4):2472-81 Authors: Valentini LC, Mangolin CA, Collet SA, Machado MF Abstract We used native polyacrylamide gel electrophoresis to identify polymorphism levels in α- and β-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-grower cooperative (CD), by EMBRAPA (BR), and "Roundup Ready" (RR) cultivars. Esterase isozymes recorded with α-naphthyl acetate and β-naphthyl acetate were produced from 14 loci. Two to three allelic variants... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375809 Fri, 04 Nov 2011 22:25:04 +0100 5375809 http://www.medworm.com/index.php?rid=5375808&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009860%26dopt%3DAbstract Genetic variability of an endangered Bromeliaceae species (Pitcairnia albiflos) from the Brazilian Atlantic rainforest. Genet Mol Res. 2011;10(4):2482-91 Authors: Domingues R, Machado MA, Forzza RC, Melo TD, Wohlres-Viana S, Viccini LF Abstract Pitcairnia albiflos is a Bromeliaceae species endemic to Brazil that has been included as data-deficient in the extinction risk list of Brazilian flora. We analyzed genetic variability in P. albiflos populations using RAPD markers to investigate population structure and reproductive mechanisms and also to evaluate the actual extinction risk level of this species. Leaves of 56 individuals of P. albiflos from three populations were collected: Urca Hill (UH, 20 individuals), Chacrinha State Park (CSP, 24 individuals) and Tijuca National... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375808 Fri, 04 Nov 2011 22:25:04 +0100 5375808 http://www.medworm.com/index.php?rid=5375807&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009861%26dopt%3DAbstract Population genetic structure of wild and hatchery black rockfish Sebastes inermis in Korea, assessed using cross-species microsatellite markers. Genet Mol Res. 2011;10(4):2492-504 Authors: An HS, Kim EM, Lee JH, Noh JK, An CM, Yoon SJ, Park KD, Myeong JI Abstract The population structure of the black rockfish, Sebastes inermis (Sebastidae), was estimated using 10 microsatellite loci developed for S. schlegeli on samples of 174 individuals collected from three wild and three hatchery populations in Korea. Reduced genetic variation was detected in hatchery strains [overall number of alleles (N(A)) = 8.07; allelic richness (A(R)) = 7.37; observed heterozygosity (H(O)) = 0.641] compared with the wild samples (overall N(A) = 8.43; A(R) = 7.83; H(O) = 0.670), but the difference w... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375807 Fri, 04 Nov 2011 22:25:04 +0100 5375807 http://www.medworm.com/index.php?rid=5375806&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009862%26dopt%3DAbstract Non-invasive sex identification of the white-bellied sea eagle (Haliaeetus leucogaster) through genetic analysis of feathers. Genet Mol Res. 2011;10(4):2505-10 Authors: Naim DM, Nor SA, Baharuddin MH Abstract The white-bellied sea eagle, Haliaeetus leucogaster, displays reversed sexual size dimorphism and is monomorphic for adult plumage coloration. Early attempts to identify sex in sexually monomorphic birds were based on morphological or chromosomal characters, but since avian W-specific DNA sequences were identified, PCR amplification has become commonly used for molecular sexing. We used a PCR test employing primers that amplify two homologous fragments of both the CHD-W gene, unique to females, and the CHD-Z gene, occurring in both sexes. This test was applied to five ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375806 Fri, 04 Nov 2011 22:25:04 +0100 5375806 http://www.medworm.com/index.php?rid=5375805&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009863%26dopt%3DAbstract Authors: Zhou JP, Zhu XP, Zhang W, Qin F, Zhang SW, Jia ZH Abstract The most important traits of Chinese Liaoning cashmere goat fiber are fiber diameter, weight, and length. We looked for polymorphisms and their possible association with cashmere fiber traits in the 5' upstream region (5' UTR) of the prolactin receptor gene (PRLR), which encodes an anterior pituitary peptide hormone involved in different physiological activities; it is the principal endocrine regulator in pelage replacement in mammals. A novel single-nucleotide polymorphism (SNP) was found in the 5' UTR of PRLR by PCR-RFLP in an analysis of 590 goats. Two genotypes (CC and CT) were observed. The frequencies of allele C and T were 0.93 and 0.07, respectively. Association analysis revealed that the PRLR 5' UTR polymo... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375805 Fri, 04 Nov 2011 22:25:04 +0100 5375805 http://www.medworm.com/index.php?rid=5375804&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009864%26dopt%3DAbstract Two novel NPHS1 mutations in a Chinese family with congenital nephrotic syndrome. Genet Mol Res. 2011;10(4):2517-22 Authors: Wu LQ, Hu JJ, Xue JJ, Liang DS Abstract Congenital nephrotic syndrome of the Finnish type (CNF) is a lethal, autosomal recessive disorder mainly caused by mutations in the NPHS1 gene; it is found at a relatively high frequency in Finns. We investigated the disease-causing mutations in a Chinese family with CNF and developed a prenatal genetic diagnosis for their latest pregnancy. Mutation analysis was made of all exons and exon/intron boundaries of NPHS1 in the fetus, parents and 50 unrelated controls using PCR and direct sequencing. A heterozygous nonsense mutation within exon 20 (c.2783C>A) and a missense mutation within exon 17 (c.2225T>C) in... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375804 Fri, 04 Nov 2011 22:25:04 +0100 5375804 http://www.medworm.com/index.php?rid=5375803&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009865%26dopt%3DAbstract Isolation and characterization of polymorphic microsatellite loci in Aleurodicus dispersus (Hemiptera, Aleyrodidae). Genet Mol Res. 2011;10(4):2523-6 Authors: Ma GC, Wu X, Ma CL, Wu HL, Hu HY, Niu LM, Fu YG Abstract Ten microsatellite markers were isolated and characterized from Aleurodicus dispersus, the spiraling whitefly, an exotic pest species that is considered to be one of the most serious agricultural pests on Hainan Island, China. The polymorphism of these loci was examined in individual whiteflies from Hainan Island and from the Canary Islands. All loci were polymorphic, with two to four alleles per locus. Mean observed and expected heterozygosity values were 0.773 and 0.585, respectively. These microsatellite markers provide powerful tools for ecological, epidemio... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375803 Fri, 04 Nov 2011 22:25:04 +0100 5375803 http://www.medworm.com/index.php?rid=5375802&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22009866%26dopt%3DAbstract Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection. Genet Mol Res. 2011;10(4):2527-33 Authors: Del Puerto HL, Martins AS, Braz GF, Alves F, Heinemann MB, Rajão DS, Araújo FC, Martins SF, Nascimento DR, Leite RC, Vasconcelos AC Abstract We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a control. Total RNA was isolated from Vero cells using 1 mL Trizol(�... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375802 Fri, 04 Nov 2011 22:25:04 +0100 5375802 http://www.medworm.com/index.php?rid=5375789&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033936%26dopt%3DAbstract This study facilitates the application of microsatellite technology in population studies by examining common sources of genotyping error, identifying unreported problems with microsatellites, and offering solutions to prevent error and bias in population studies. PMID: 22033936 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375789 Fri, 04 Nov 2011 22:25:04 +0100 5375789 http://www.medworm.com/index.php?rid=5375788&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033937%26dopt%3DAbstract Optimization of PCR conditions to amplify Cyt b, COI and 12S rRNA gene fragments of Malayan gaur (Bos gaurus hubbacki) mtDNA. Genet Mol Res. 2011;10(4):2554-68 Authors: Rosli MK, Zamzuriada AS, Syed-Shabthar SM, Mahani MC, Abas-Mazni O, Md-Zain BM Abstract PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine the best conditions of PCR mixture and profile to obtain the best PCR products for sequencing purposes. Four selected target factors for enhancing PCR, a... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375788 Fri, 04 Nov 2011 22:25:04 +0100 5375788 http://www.medworm.com/index.php?rid=5375787&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033938%26dopt%3DAbstract This study demonstrated that variations in the C3435T gene play an important role in the pathogenesis of infantile spasms in the Han Chinese population; 3435TT is associated with increased risk of having this epilepsy syndrome. PMID: 22033938 [PubMed - in process] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375787 Fri, 04 Nov 2011 22:25:04 +0100 5375787 http://www.medworm.com/index.php?rid=5375786&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033939%26dopt%3DAbstract We examined the sensitivity, specificity and accuracy of real-time qPCR, without pre-enrichment or selective enrichment stages, for the quantification of S. enterica serovar Enteritidis in resistant mice, as a model of asymptomatic carrier animal. The results were compared with those obtained by traditional bacteriological culture methods, the gold standard test. Two hundred and forty-three samples, including spleen, liver, mesenteric lymph nodes, portions of intestine, intestinal content of the ileocecal portion, and feces, were collected from a group of 27 C57BL/6 mice, that had been intragastrically inoculated with high doses of S. enterica serovar Enteritidis. The real-time qPCR assay presented a consistent linearity of the standard curve (r(2) = 0.999), with very low differences betwe... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375786 Fri, 04 Nov 2011 22:25:04 +0100 5375786 http://www.medworm.com/index.php?rid=5375794&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033907%26dopt%3DAbstract Sexing single bovine blastomeres using TSPY gene amplification. Genet Mol Res. 2011 Oct 25;10(4) Authors: Carneiro MC, Takeuchi PL, Araújo A, Lôbo RB, Elias FP, Vila RA, Miranda-Furtado CL, Ramos ES Abstract The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same emb... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375794 Tue, 25 Oct 2011 04:00:00 +0100 5375794 http://www.medworm.com/index.php?rid=5375793&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033908%26dopt%3DAbstract Genetic dissimilarity of putative gamma-ray-induced 'Preciosa - AAAB-Pome type' banana (Musa sp) mutants based on multivariate statistical analysis. Genet Mol Res. 2011 Oct 25;10(4) Authors: Pestana RK, Amorim EP, Ferreira CF, Amorim VB, Oliveira LS, Ledo CA, Silva SO Abstract Bananas are among the most important fruit crops worldwide, being cultivated in more than 120 countries, mainly by small-scale producers. However, short-stature high-yielding bananas presenting good agronomic characteristics are hard to find. Consequently, wind continues to damage a great number of plantations each year, leading to lodging of plants and bunch loss. Development of new cultivars through conventional genetic breeding methods is hindered by female sterility and the low number of seeds. Mu... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375793 Tue, 25 Oct 2011 04:00:00 +0100 5375793 http://www.medworm.com/index.php?rid=5375792&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033909%26dopt%3DAbstract A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis. Genet Mol Res. 2011 Oct 25;10(4) Authors: Fang DA, Wang Q, Wang J, He L, Liu LH, Wang Y Abstract The freshwater crayfish Cherax quadricarinatus, originally from Australia, is an invasive species that is also widely used in aquaculture. DEAD-box helicase family genes are found throughout evolution and encode RNA-binding proteins. The human DDX5 (p68) is important for normal cell growth, differentiation and proliferation. We identified a C. quadricarinatus homolog of DDX5 (Cq-DDX5); the temporal expression of Cq-DDX5 mRNA transcripts was measured during early ontogenesis, during spermatogenesis, during testes development, and during the annual cycle.... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375792 Tue, 25 Oct 2011 04:00:00 +0100 5375792 http://www.medworm.com/index.php?rid=5375791&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033910%26dopt%3DAbstract Application of the Sleeping Beauty system in Saanen goat fibroblast cells for establishing persistent transgene expression. Genet Mol Res. 2011 Oct 25;10(4) Authors: Jiang BC, Kaleri HA, Zhang HX, Chen J, Liu HL Abstract The Sleeping Beauty (SB) transposon system is a promising new method for establishing persistent transgene expression in vivo. We applied the SB system for enhancing transgenesis in Saanen dairy goat fibroblast cells. We constructed a pKT2/CMV-EGFP-IRES-PURO vector and investigated the influence of transposon and transposase vector ratios on transfection efficiency in the Saanen goat fibroblast cells. To enhance SB system performance, we developed a new transfection technique (double-transfection method) for the SB system. The cultured cells were transfecte... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375791 Tue, 25 Oct 2011 04:00:00 +0100 5375791 http://www.medworm.com/index.php?rid=5375790&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033911%26dopt%3DAbstract Authors: Almeida LM, Basu U, Williams JL, Moore SS, Guan LL Abstract Bovine spongiform encephalopathy (BSE) is a fatal disorder in cattle characterized by progressive neurodegeneration of the central nervous system. We investigated the molecular mechanisms involved in neurodegeneration during prion infection through the identification of genes that are differentially expressed (DE) between experimentally infected and non-challenged cattle. Gene expression of caudal medulla from control and orally infected animals was compared by microarray analysis using 24,000 bovine oligonucleotides representing 16,846 different genes to identify DE genes associated with BSE disease. In total, 182 DE genes were identified between normal and BSE-infected tissues (>2.0-fold change, P < 0.01);... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375790 Tue, 25 Oct 2011 04:00:00 +0100 5375790 http://www.medworm.com/index.php?rid=5375801&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033900%26dopt%3DAbstract This study presented an inducible recombinant gene expression system, also providing more gene regulation elements with potential for biotechnological applications. PMID: 22033900 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375801 Fri, 21 Oct 2011 04:00:00 +0100 5375801 http://www.medworm.com/index.php?rid=5375800&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033901%26dopt%3DAbstract Cytochrome c oxidase subunit I barcoding of the green bee-eater (Merops orientalis). Genet Mol Res. 2011 Oct 21;10(4) Authors: Arif IA, Khan HA, Shobrak M, Williams J Abstract DNA barcoding using mitochondrial cytochrome c oxidase subunit I (COI) is regarded as a standard method for species identification. Recent reports have also shown extended applications of COI gene analysis in phylogeny and molecular diversity studies. The bee-eaters are a group of near passerine birds in the family Meropidae. There are 26 species worldwide; five of them are found in Saudi Arabia. Until now, GenBank included a COI barcode for only one species of bee-eater, the European bee-eater (Merops apiaster). We sequenced the 694-bp segment of the COI gene of the green bee-eater M. orientalis and ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375800 Fri, 21 Oct 2011 04:00:00 +0100 5375800 http://www.medworm.com/index.php?rid=5375799&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033902%26dopt%3DAbstract Leptin mRNA expression in the rat mammary gland at different activation stages. Genet Mol Res. 2011 Oct 21;10(4) Authors: Wang YY, Wang YL, Li HP, Zhu HS, Jiang QD, Zhang L, Wang LF, Han LQ, Zhong K, Guo YJ, Lu WF, Li HJ, Yang GY Abstract Leptin is expressed in various tissues, suggesting that this protein is effective not only at the central nervous system level, but also peripherically. Recent studies have shown leptin production by other tissues, including the placenta, stomach, and mammary tissues, but there is no information available concerning expression levels of leptin in the rat mammary gland at different activation stages. We used semi-quantitative RT-PCR to investigate leptin mRNA expression levels in the rat mammary gland at different activity stages. Rat mamma... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375799 Fri, 21 Oct 2011 04:00:00 +0100 5375799 http://www.medworm.com/index.php?rid=5375798&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033903%26dopt%3DAbstract Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance. Genet Mol Res. 2011 Oct 21;10(4) Authors: Polizel AM, Medri ME, Nakashima K, Yamanaka N, Farias JR, de Oliveira MC, Marin SR, Abdelnoor RV, Marcelino-Guimarães FC, Fuganti R, Rodrigues FA, Stolf-Moreira R, Beneventi MA, Rolla AA, Neumaier N, Yamaguchi-Shinozaki K, Carvalho JF, Nepomuceno AL Abstract We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375798 Fri, 21 Oct 2011 04:00:00 +0100 5375798 http://www.medworm.com/index.php?rid=5375797&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033904%26dopt%3DAbstract Transcription factors expressed in soybean roots under drought stress. Genet Mol Res. 2011 Oct 21;10(4) Authors: Pereira SS, Guimarães FC, Carvalho JF, Stolf-Moreira R, Oliveira MC, Rolla AA, Farias JR, Neumaier N, Nepomuceno AL Abstract To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed their expression levels under drought stress. The NCBI software was used to find the predicted amino acid sequences of the transcription factors, and the Clustal X software was used to align s... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375797 Fri, 21 Oct 2011 04:00:00 +0100 5375797 http://www.medworm.com/index.php?rid=5375796&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033905%26dopt%3DAbstract In conclusion, this meta-analysis suggests that CDSN -619C/T polymorphism may not be associated with susceptibility to psoriasis. PMID: 22033905 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375796 Fri, 21 Oct 2011 04:00:00 +0100 5375796 http://www.medworm.com/index.php?rid=5375795&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22033906%26dopt%3DAbstract In this study, we investigated the possibility of myostatin being an indicator of denervated muscle atrophy. ELISA was used to detect the concentration of myostatin and follistatin in sera collected from individual rats at different times after sciatic nerve crush. A strong correlation was shown between the expression level of secreted myostatin in circulation and the wet weight ratio of the gastrocnemius muscle. The ratio of follistatin/myostatin could be used to monitor the progress of target muscle atrophy and recovery. Our study provides a potential serological test to detect denervated muscle atrophy for clinical purposes. PMID: 22033906 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research) Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5375795 Fri, 21 Oct 2011 04:00:00 +0100 5375795 http://www.medworm.com/index.php?rid=5293463&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21968620%26dopt%3DAbstract Authors: Silveira CA, Daldegan MB, Ferrari I Abstract We analyzed the results of treatment with imatinib mesylate in 70 patients with chronic-phase chronic myeloid leukemia who had previously been treated (with second-line or higher imatinib), many of them in a late chronic phase. The median follow-up period was 60.5 months (range 3-100 months). Our objective was to assess the efficacy and safety of treatment. The mean dose was 400 mg per day. The hematologic response rate was 92.1% at six months, while the cumulative rates of major and complete cytogenetic responses were 73.6 and 66.3%, respectively. Molecular response rate improved slowly and steadily over time, reaching 65.8% at 60 months, remaining stable for up to 96 months. The five-year progression-free survival and overall ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5293463 Fri, 07 Oct 2011 19:20:02 +0100 5293463 http://www.medworm.com/index.php?rid=5293462&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21968621%26dopt%3DAbstract Authors: Dhawan SS, Rai GK, Darokar MP, Lal RK, Misra HO, Khanuja SP Abstract Velvet bean (Mucuna pruriens) seeds contain the catecholic amino acid L-DoPA (L-3,4-dihydroxyphenylalanine), which is a neurotransmitter precursor and used for the treatment of Parkinson's disease and mental disorders. The great demand for L-DoPA is largely met by the pharmaceutical industry through extraction of the compound from wild populations of this plant; commercial exploitation of this compound is hampered because of its limited availability. The trichomes present on the pods can cause severe itching, blisters and dermatitis, discouraging cultivation. We screened genetic stocks of velvet bean for the trichome-less trait, along with high seed yield and L-DoPA content. The highest yielding trichome-... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5293462 Fri, 07 Oct 2011 19:20:02 +0100 5293462 http://www.medworm.com/index.php?rid=5293461&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21968622%26dopt%3DAbstract Authors: Pinto LF, Ferraz JB, Pedrosa VB, Eler JP, Meirelles FV, Bonin MN, Rezende FM, Carvalho ME, Cucco DC, Silva RC Abstract We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms T945M, GHR2, E2FB, and CAPN4751 were evaluated. All genotypic classes were observed; however, the T/T genotype of T945M and E2FB was found at a low frequency. A significant association of E2FB with drip loss (a measure of water-holding capacity) was detected at seven days of ... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5293461 Fri, 07 Oct 2011 19:20:02 +0100 5293461 http://www.medworm.com/index.php?rid=5293460&cid=s_28401_50_f&fid=28401&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21968623%26dopt%3DAbstract Authors: Bai CC, Liu SF, Zhuang ZM, Lin L, Wang H, Chang YQ Abstract The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)(13)-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. The observed and expected heterozygosities ranged from 0.0244 to 0.7660 (mean 0.4194) and 0.0244 to 0.8758 (mean 0.5002), respectively. Polymorphism at these loci indicated from two to 15 alleles (mean 5.7); 14 of 18 loci conformed to Hardy-Weinberg equilibrium. These markers should be useful for management and conservation studies of this species.... Genetics and Molecular Research journals http://www.medworm.com/rss/comments.php?id=5293460 Fri, 07 Oct 2011 19:20:02 +0100 5293460