MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Clinical and Experimental Pathology' source. Thu, 09 Feb 2012 00:42:12 +0100 http://www.medworm.com/index.php?rid=5665163&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295141%26dopt%3DAbstract Authors: Loyd CM, Diaconu D, Fu W, Adams GN, Brandt E, Knutsen DA, Wolfram JA, McCormick TS, Ward NL Abstract VEGF and Angiopoietin (Ang)1 are growth factors that independently improve wound healing outcomes. Using a tet-repressible mouse model coupled with streptozotocin-induced diabetes, we examined wound healing in diabetic and nondiabetic mice engineered to overexpress keratinocyte-specific (K5) VEGF, Ang1 or Ang1-VEGF combined. All nondiabetic mice healed more rapidly than their diabetic counterparts; however overexpression of VEGF, Ang1 or the combination failed to improve wound closure under diabetic conditions. Conversely, under nondiabetic conditions, combining Ang1 and VEGF resulted in rapid wound closure. Molecular analyses of diabetic and nondiabetic K5-Ang1-VEGF skin r... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665163 Tue, 07 Feb 2012 13:36:02 +0100 5665163 http://www.medworm.com/index.php?rid=5665162&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295142%26dopt%3DAbstract CONCLUSION: this study points to extramural growth as a predictor of peritoneal recurrence in GIST, probably as a consequence of tumor rupture or due to microscopic serosal penetration. This study aimed at alerting surgical pathologists to the importance of careful gross and microscopic assessment of resection specimen harboring GIST to allow for reliable prospective evaluation of serosal involvement as an adverse prognostic factor in GIST. PMID: 22295142 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665162 Tue, 07 Feb 2012 13:36:02 +0100 5665162 http://www.medworm.com/index.php?rid=5665161&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295143%26dopt%3DAbstract Authors: Ohgami N, Tamura H, Ohgami K, Iida M, Yajima I, Kumasaka MY, Goto Y, Sone M, Nakashima T, Kato M Abstract About 120 million people worldwide suffer from congenital (early-onset) hearing loss. Thirty percent of them have syndromic hearing loss and the remaining 70% have non-syndromic hearing loss. In addition, a large number of elderly people worldwide suffer from age-related (late-onset) hearing loss. c-Ret and c-RET have been shown to be essential for the development and maintenance of neurons including the enteric nervous system (ENS) in mice and humans. Impairments of endothelin receptor B (EDNRB) and SOX10 have been shown to cause a significantly increased risk of dominant sensorineural deafness in Hirschsprung disease (HSCR) patients. We have recently shown that impai... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665161 Tue, 07 Feb 2012 13:36:02 +0100 5665161 http://www.medworm.com/index.php?rid=5665160&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295144%26dopt%3DAbstract Authors: Dorn C, Heilmann J, Hellerbrand C Abstract Xanthohumol, the major prenylated chalcone found in hops, is known for its anti-inflammatory properties. We have recently shown that xanthohumol inhibits hepatic inflammation and fibrosis in a murine model of nonalcoholic steatohepatitis. The aim of this study was to investigate the effect of xanthohumol in an acute model of liver injury. Carbon tetrachloride (CCl(4)), an industrial solvent, is a hepatotoxic agent and its administration is widely used as an animal model of toxin-induced liver injury. Xanthohumol was applied orally at a dose of 1 mg/g body weight 2 days prior as well as during and after exposure to CCl(4). 72 h after a single CCl(4) application histomorphology and serum levels of transaminases revealed considerable... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665160 Tue, 07 Feb 2012 13:36:02 +0100 5665160 http://www.medworm.com/index.php?rid=5665159&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295145%26dopt%3DAbstract Authors: Khabele D, Fadare O, Liu AY, Wilson AJ, Wass E, Osteen K, Crispens MA Abstract Fallopian tube carcinoma (FTCA) is a very rare cancer type, but may be a useful platform for investigating high grade serous tumors of the pelvis that originate from a serous tubal intraepithelial carcinoma (STIC) precursor. Metastatic tumors from a patient diagnosed with Stage IIIC high grade serous FTCA (P0) were transplanted via intraperitoneal (IP) injection into a small cohort of mice (passage, P1). Patient information was obtained from the medical record. Tumors were grown, harvested and re-implanted or archived through P3. The P3 cohort was treated with saline (n=8) or cisplatin, 5 mg/kg (n=8), weekly for 4 weeks. After sacrifice, tumors from each passage and treatment group were passaged... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665159 Tue, 07 Feb 2012 13:36:02 +0100 5665159 http://www.medworm.com/index.php?rid=5665158&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295146%26dopt%3DAbstract Authors: Terada T Abstract The author investigated histopathology of 615 consecutive duodenal specimens in our pathology laboratory. Computer search of the duodenal lesions was performed. Review of histological slides was done, when appropriate. The duodenal specimens were composed of 567 benign lesions and 48 malignant lesions. The 567 benign lesions were composed of chronic non-specific duodenitis in 334 cases (60.0%), duodenal ulcer in 101 cases (17,8%), heterotopic gastric mucosa in 81 cases (14.3%), hyperplastic polyp in 16 cases (2.8%), Brunner's gland hyperplasia in 14 cases (2.5%), Brunner's gland adenoma in 8 cases (1.4%), lymphoid polyp in 5 cases (0.8%), tubular adenoma in 4 cases (0.7%), lymphangioma in 2 cases (0.4%), endocrine nests in 1 case (0.2%), and amyloidosis i... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665158 Tue, 07 Feb 2012 13:36:02 +0100 5665158 http://www.medworm.com/index.php?rid=5665157&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295147%26dopt%3DAbstract Authors: Terada T Abstract The author investigated histopathology of 615 consecutive duodenal specimens in our pathology laboratory in Japan. A computer review of the duodenal specimens was done. In cases of malignancy, histological slides were reviewed. The duodenal specimens were composed of 567 benign lesions (92%) and 48 malignant lesions (8%). The 48 malignant lesions were composed of 20 cases (42%) of primary adenocarcinoma, 10 cases (21%) of primary adenocarcinoma of ampulla Vater, 4 cases (8%) of primary squamous cell carcinoma, 1 (2%) cases of primary spindle cell carcinoma, 4 (8%) cases of carcinoid tumors, 1 (2%) case of malignant lymphoma, and 8 cases (17%) of secondary carcinoma from the pancreatic carcinoma or bile duct carcinoma. The primary adenocarcinoma (n=20) was... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665157 Tue, 07 Feb 2012 13:36:02 +0100 5665157 http://www.medworm.com/index.php?rid=5665156&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295148%26dopt%3DAbstract CONCLUSIONS: The pathogenesis of IPF is complex and involves multiple factors, possibly including EMT. Histological analysis suggests TGF-β-stimulated myofib rob lasts initiate a contractile response within established fibroblastic foci while proliferating ATII cells attempt to instigate alveolar epithelium repair. Marker expression (N-cadherin and Ki-67) correlation with histological disease activity (as reflected by fibroblastic foci extent) may emerge as future prognostic indicators for IPF. PMID: 22295148 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665156 Tue, 07 Feb 2012 13:36:02 +0100 5665156 http://www.medworm.com/index.php?rid=5665155&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295149%26dopt%3DAbstract We report a case of a primary central nervous system lymphoma (PCNSL) with findings consistent with DLBCL/BL. It is characterized by a very aggressive clinical course, and a widespread multifocal involvement of the CNS. Our case shows that a DLBCL/BL can manifest in the CNS alone without any systemic involvement. PMID: 22295149 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665155 Tue, 07 Feb 2012 13:36:02 +0100 5665155 http://www.medworm.com/index.php?rid=5665154&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295150%26dopt%3DAbstract We report herein a first case of endometrial dedifferentiated endometrioid carcinoma in a 51-year old woman in Chinese population. We performed immunoperoxidase studies for 12 markers. Among them, cytokeratins, keratin 7, keratin 18, EMA, estrogen receptor (ER), progesterone receptor (PR), and vimentin show significantly differential expression between differentiated and undifferentiated area. PMID: 22295150 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665154 Tue, 07 Feb 2012 13:36:02 +0100 5665154 http://www.medworm.com/index.php?rid=5665153&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295151%26dopt%3DAbstract Authors: Terada T Abstract The author reports a very rare case of spindle cell carcinoma (SpCC) of the urinary bladder progressed from ordinary papillary transitional cell carcinoma (TCC). A 63-year-old man complained of hematuria. A transurethral endoscopic examination revealed a papillary tumor, and transuthetral resection of bladder tumor (TUR-BT) was performed and was diagnosed as ordinary papillary urothelial TCC. Since then, he was treated with TUR-BT eight times. Chemotherapy, radiation, radical cystectomy and lymph nodes dissection were performed 16 years after the first TUR-BT. However, he developed rectal mucosal metastasis. He is now alive 17 years after the first presentation. All the TUR-BT specimens were ordinary papillary TCCs without invasion (pTa). Immunohistochemi... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665153 Tue, 07 Feb 2012 13:36:02 +0100 5665153 http://www.medworm.com/index.php?rid=5665152&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295152%26dopt%3DAbstract We present a case of a 70-year-old HIV negative man with a five-year history of progressive dysnomia and new onset right extremity numbness, dysarthria, and blurry vision. On magnetic resonance imaging (MRI), an infiltrative enhancing tumor was noted. Follow up brain biopsy results revealed a small lymphocytic infiltrate with scattered plasma cells in a predominantly perivascular growth pattern. Flow-cytometric findings revealed a lambda monotypic B-cell population. The morphology and the flow cytometric findings were consistent with involvement by a low grade B-cell lymphoma. Subsequent positron emission tomography (PET) studies along with bone marrow biopsy and serum protein electrophoresis showed no evidence of systemic disease. The above findings are consistent with involvement by a no... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665152 Tue, 07 Feb 2012 13:36:02 +0100 5665152 http://www.medworm.com/index.php?rid=5665151&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295153%26dopt%3DAbstract Authors: Terada T Abstract Recently, several cases of intraductal oncocytic papillary neoplasm (IOPN) of the liver and hepatic bile ducts have been reported. The author herein reports the first case of IOPN of the common bile duct (CBD). A 78-year-old man was admitted to our hospital because of jaundice. Imaging modalities including US, CT, MRI revealed an intraductal tumor of the middle CBD and biliary dilation distal to the tumor. A partial resection of the CBD was performed. Grossly, a papillary tumor measuring 20 × 15 mm was found within the CBD. Mucus is absent. Histologically, the papillary tumor was composed of atypical oncocytes. The atypia was enough to be diagnosed as adenocarcinoma. No invasive features were noted. Immunohistochemically, the tumor cells were positive fo... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665151 Tue, 07 Feb 2012 13:36:02 +0100 5665151 http://www.medworm.com/index.php?rid=5665150&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295154%26dopt%3DAbstract We described the case of a 60-year-old female patient who developed bullous pemphigoid for 3 month and presented with bleeding tendency and hematoma in the tongue. Therapy with methylprednisolone, cyclophosphamide, intravenous immunoglobulin and factor VIII reposition was instituted, resulting in a remission of the bleeding and negativity for antibodies against factor VIII titers. We concluded that, despite its rarity, the presence of acquired factor VIII inhibitors should be investigated when patients with autoimmune diseases develop bleeding manifestations. PMID: 22295154 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665150 Tue, 07 Feb 2012 13:36:02 +0100 5665150 http://www.medworm.com/index.php?rid=5665149&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295155%26dopt%3DAbstract Authors: Delaney D, Chetty R Abstract An 85-year old female had a polypoid tumour in the sigmoid colon that histologically conformed to a lymphoepithelioma-like (LEL) carcinoma. The tumour was arranged in cords, chains, clusters and microalveoli of pleomorphic, irregular cells set within a dense intratumoral lymphocytic stroma. The tumour was EBV-negative and showed loss of MLH-1 and PMS-2 mismatch repair proteins. The patient did not fulfil the criteria for HNPCC. Only 5 other cases of primary colonic LEL carcinoma have been described previously and only one case appears to have an unequivocal association with EBV. In addition, one of the cases was encountered in a HNPCC patient. This is an unusual morphologic variant of a microsatellite unstable tumour with a LEL pattern, not ass... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5665149 Tue, 07 Feb 2012 13:36:02 +0100 5665149 http://www.medworm.com/index.php?rid=5484244&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135720%26dopt%3DAbstract Authors: Dhingra S, Feng W, Brown RE, Zhou Z, Khoury T, Zhang R, Tan D Abstract Cancer stem cells (CSC) are unique subpopulations that have the capacity to drive malignant progression and mediate radio/chemoresistance. The role of nestin as a CSC marker in gastric adenocarcinoma is largely unknown. Our objective was to evaluate immunoexpression of CSC markers CD44 and nestin in gastric adenocarcinoma versus non-neoplastic gastric mucosae (NNGM) and correlate it with various clinicopathologic factors. Tissue microarray blocks from 174 cases of gastric adenocarcinoma and 41 samples of adjacent NNGM were assembled. Clinical data including patient's age and sex, tumor histologic subtype and grade, and disease stage were obtained. Expression of CD44 and nestin was assessed by immunohist... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484244 Thu, 08 Dec 2011 17:54:02 +0100 5484244 http://www.medworm.com/index.php?rid=5484243&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135721%26dopt%3DAbstract Authors: Anani W, Bruggeman R, Zander DS Abstract Benign and malignant pleural processes display a large and overlapping spectrum of morphological appearances, and can be difficult to distinguish, histologically, from each other. β-catenin, a participant in the wingless-type (Wnt) transduction pathway, is involved in the pathogenesis of malignant mesothelioma and has received limited evaluation for its ability to serve as a diagnostic aid for distinguishing between individual pleural disorders. We performed immunohistochemistry for β-catenin on 10 pleural malignant mesotheliomas, 10 examples of mesothelial hyperplasia and 18 cases of organizing pleuritis. Although differences were noted in staining intensity between the mesothelioma and mesothelial hyperplasia groups, extensivene... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484243 Thu, 08 Dec 2011 17:54:02 +0100 5484243 http://www.medworm.com/index.php?rid=5484242&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135722%26dopt%3DAbstract Authors: Suh-Lailam BB, Chiaro TR, Davis K W, Wilson AR, Tebo AE Abstract The high avidity (HA) anti-dsDNA IgG ELISA is considered highly specific for the diagnosis of systemic lupus erythematosus (SLE). The main objective of this study was to determine the performance of this test with existing assays for detecting anti-dsDNA IgG antibodies as well as assess its analytical characteristics. For method comparison studies, we investigated the correlation between the HA ELISA with 8 other assays for the detection of dsDNA IgG antibodies namely; six anti-dsDNA IgG ELISA, the Crithidia luciliae immunofluorescence test (CLIFT) and an in-house developed Farr radioimmunoassay (RIA). Overall, 125 patient (100 ANA-positive, 25 CLIFT-tested) and 100 healthy control samples were tested. The as... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484242 Thu, 08 Dec 2011 17:54:02 +0100 5484242 http://www.medworm.com/index.php?rid=5484241&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135723%26dopt%3DAbstract In conclusion, overexpressions of FASN and p-c-Met were observed in a subset of thyroid carcinomas of follicular origin, which may be of values for targeted therapy and predicting prognosis while the positive immunostaining for these immunomarkers may be nonspecific for Hürthle cell thyroid carcinomas. PMID: 22135723 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484241 Thu, 08 Dec 2011 17:54:02 +0100 5484241 http://www.medworm.com/index.php?rid=5484240&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135724%26dopt%3DAbstract This study suggests that HIF-1α may regulate the expression of EPO, which plays a favorable regulatory role in the proliferation and survival of human first-trimester trophoblast cells and DSCs via inactivating p38 and activating STAT5 in an autocrine manner, while the inadequate EPO expression at maternal-fetal interface may lead to pregnancy wastage in humans. PMID: 22135724 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484240 Thu, 08 Dec 2011 17:54:02 +0100 5484240 http://www.medworm.com/index.php?rid=5484239&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135725%26dopt%3DAbstract Authors: Miiji LN, Petrilli AS, Di Cesare S, Odashiro AN, Burnier MN, de Toledo SR, Garcia RJ, Alves MT Abstract Biologic agents targeting oncogenes have encourage researchs trying to correlate the role of tyrosine kinase in the pathogenesis of tumours. Osteosarcoma is a high grade aggressive neoplasm with poor survival. Our aim was to investigate c-kit immunoexpression, its prognostic relevance for patients with osteosarcoma, and the effect of imatinib mesylate (STI571) on proliferation and invasion of the human osteosarcoma cell line.A retrospective immu-nohistochemical study was performed on archival formalin-fixed paraffin-embedded specimens from 52 patients with high-grade primary osteosarcoma of extremities treated at the Pediatric Oncology Institute (IOP, GRAAC) and archived... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484239 Thu, 08 Dec 2011 17:54:02 +0100 5484239 http://www.medworm.com/index.php?rid=5484238&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135726%26dopt%3DAbstract Authors: Shen Q, Duan X, Feng W, Nguyen N, Lapus A, Brown RE, Chen L Abstract Intravascular large B-cell lymphoma (IVLBCL) is a rare, aggressive and often fatal non-Hodgkin lymphoma characterized by preferential growth of malignant B-cells within the lumina of small vessels. Rituximab plus anthracy-cline-based chemotherapy is the current standard regimen for IVLBCL, however it has minimal efficacy in relapsed or refractory diseases. Recent clinical trials have shown a significant anti-lymphoma activity of mammalian target of rapamycin (mTOR) inhibitors in relapsed and refractory diffuse large B-cell lymphoma (DLBCL); however, the activation status of the mTOR pathway and the therapeutic potential of mTOR inhibitors in IVLBCL have not yet been studied. Here we described the clinicop... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484238 Thu, 08 Dec 2011 17:54:02 +0100 5484238 http://www.medworm.com/index.php?rid=5484237&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135727%26dopt%3DAbstract Authors: Terada T Abstract Angiosarcoma of the oral cavity is extremely rare. A 77-year-old woman consulted to our hospital because of polypoid mass of the mandibular gingival. Physical examination showed polypoid reddish mass measuring 1.5 × 1.5 × 1 cm in the mandibular gingival posterior to the front tooth. Enucleation of the tumor was performed. Grossly, the tumor was not encapsulated. Histologically, the tumor consisted of atypical spindle with hyperchromatic nuclei with nucleoli. Mitotic figures were scattered. Vasoformative channels were present in some areas. The surgical margins were positive. Immunohistochemically, the tumor cells were positive for factor VIII-related antigen, CD31, CD34, vimentin, p53 protein, but negative for pancytokeratin (AE1/3 and CAM5.2), S100 pro... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484237 Thu, 08 Dec 2011 17:54:02 +0100 5484237 http://www.medworm.com/index.php?rid=5484236&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135728%26dopt%3DAbstract Authors: Terada T Abstract Herein reported is a case of papillary squamous cell carcinoma (PSCC) in the oral cavity with features of koilocytosis, acantholysis and pseudovascular structure. A 73-year-old woman consulted to our hospital because of a tumor in the right mandibular gum. Physical examination revealed an exophytic papillary tumor of the right mandibu-lar gum, and a biopsy was performed. The biopsy revealed squamous cell carcinoma. No metastases were found by various imaging techniques. Therefore, resection of the tumor and mandibular bone was performed. Grossly, the tumor was exophytic and papillary, and measured 2 × 2 × 1 cm. The mandibular bone was free from tumor invasion. Microscopically, the tumor showed exophytic papillary proliferation with fibrovascular cores a... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484236 Thu, 08 Dec 2011 17:54:02 +0100 5484236 http://www.medworm.com/index.php?rid=5484235&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135729%26dopt%3DAbstract Authors: Terada T Abstract Gastric adenocarcinoma of fundic gland type is extremely rare. A 78-year-old Japanese man with carcinomatosis of unknown primary origin underwent upper gastrointestinal endoscopy. The endoscopy revealed an elevated lesion with central ulcer (1.3 × 2 × 1.5 cm) in the gastric corpus of fundic gland area. One biopsy was taken. The biopsy showed well differentiated adenocarcinoma. The adenocarcinoma was composed of basophilic chief cell-like cells and acidophilic parietal cells. The biopsy diagnosis was well differentiated adenocarcinoma of the stomach composed of chief cell-like cells and parietal cells (gastric adenocarcinoma of fundic gland type). PMID: 22135729 [PubMed - in process] (Source: International Journal of Clinical and Experimental Patholo... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484235 Thu, 08 Dec 2011 17:54:02 +0100 5484235 http://www.medworm.com/index.php?rid=5484234&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22135730%26dopt%3DAbstract In conclusion, a very rare case of DLBCL of the ethmoid sinus was reported. PMID: 22135730 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5484234 Thu, 08 Dec 2011 17:54:02 +0100 5484234 http://www.medworm.com/index.php?rid=5430210&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076163%26dopt%3DAbstract Authors: Uddin S, Hussain AR, Siraj AK, Khan OS, Bavi PP, Al-Kuraya KS Abstract Leptin is a multifunctional adipose-derived cytokines that play a critical role in bodyweight homeostasis and energy balance. Recently, leptin and leptin receptor dysreulation have been reported in variety of malignant cells including thyroid. Leptin modulates growth and proliferation of cancer cells via activation of various growth and survival signaling pathways including JAK/STAT, PI3-kinase/AKT and/or Map kinases. In this review, current understanding of leptin's role in the pathogenesis of thyroid cancer has been described. PMID: 22076163 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430210 Mon, 21 Nov 2011 08:24:03 +0100 5430210 http://www.medworm.com/index.php?rid=5430209&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076164%26dopt%3DAbstract Authors: Huang L, Schauer IG, Zhang J, Mercado-Uribe I, Deavers MT, Huang J, Liu J Abstract TMPRSS2:ERG is a gene fusion resulting from the chromosomal rearrangement of the androgen-regulated TMPRSS2 gene and the ETS transcription factor ERG, leading to the over-expression of the oncogenic molecule ERG. This gene rearrangement has been found in approximately half of all prostate cancers and ERG overexpression is considered as a novel diagnostic marker for prostate carcinoma. However, little is known about the role of the TMPRSS2:ERG gene fusion in ovarian cancer. The purpose of this study was to test ERG expression in ovarian cancer and its potential as a diagnostic marker for ovarian carcinoma progression. A tissue microarray containing 180 ovarian cancer tissues of various pathol... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430209 Mon, 21 Nov 2011 08:24:03 +0100 5430209 http://www.medworm.com/index.php?rid=5430208&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076165%26dopt%3DAbstract Authors: Song X, Andrew Allen R, Terence Dunn S, Fung KM, Farmer P, Gandhi S, Ranjan T, Demopoulos A, Symons M, Schulder M, Li JY Abstract Glioblastoma with primitive neuroectodermal tumor-like components (GBM-PNET), a rare variant of glioblastoma, poses both diagnostic and therapeutic challenges. Ten patients with GBM-PNET were investigated with a median age of 51.5 years and the male to female ratio of 4:1. The majority of patients (7 out of 10) showed ring-enhancing lesions on magnetic resonance imaging (MRI), which is classic for GBMs. Restricted diffusion was noted in 7 cases where diffusion weighted imaging (DWI) was performed, which correlates with the presence of PNET-like components. CD56 and vimentin immunostaining made the diagnosis of GBM-PNET much easier. Vimentin stro... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430208 Mon, 21 Nov 2011 08:24:03 +0100 5430208 http://www.medworm.com/index.php?rid=5430207&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076166%26dopt%3DAbstract Authors: Schnabl B, Czech B, Valletta D, Weiss TS, Kirovski G, Hellerbrand C Abstract Hepatocellular lipid accumulation is a hallmark of non-alcoholicfatty liver disease (NAFLD), which encompasses a spectrum ranging from simple steatosis to non-alcoholic steatohepatitis (NASH) and ultimately cirrhosis. Zinc finger protein 267 (ZNF267) belongs to the family of Kruppel-like transcription factors, which regulate diverse biological processes that include development, proliferation, and differentiation. We have previously demonstrated that ZNF267 expression is up-regulated in liver cirrhosis and is further increased in hepatocellular carcinoma (HCC). Here, we analyzed the expression of ZNF267 in tissue specimens of NAFLD patients and found a significant up-regulation compared to normal ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430207 Mon, 21 Nov 2011 08:24:03 +0100 5430207 http://www.medworm.com/index.php?rid=5430206&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076167%26dopt%3DAbstract CONCLUSION: The results show that both claudins are most commonly expressed in lung adenocarcinomas and they are more occasionally detected in other histological tumour types. Curiously, female patients and non-smokers express these claudins more commonly suggesting that they may play a part in the carcinogenesis of tobacco unrelated carcinoma. Claudin 18 associated with a better survival in lung adenocarcinoma and had an independent prognostic value and may thus be used in the evaluation of patient prognosis. PMID: 22076167 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430206 Mon, 21 Nov 2011 08:24:03 +0100 5430206 http://www.medworm.com/index.php?rid=5430205&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076168%26dopt%3DAbstract Authors: Valdivia A, Peralta R, Matute-González M, García Cebada JM, Casasola I, Jiménez-Medrano C, Aguado-Pérez R, Villegas V, González-Bonilla C, Manuel-Apolinar L, Ibáñez M, Salcedo M Abstract The metalloproteinases (MMP) 11 and 12 have been shown to be expressed in cervical cancer (CC). In order to extend our previous results, these MMPs were evaluated in cervical precursor lesions. One hundred seventeen cervical scrapes: thirty-six normal, thirty-six Low grade squamous lesions (LSIL), thirty-six High grade (HSIL), nine CC; and, also ninety-nine paraffin-embedded cervical lesions: fifteen normal cervices, thirty eight LSIL, sixteen HSIL, and five CC were collected. The samples were analyzed for relative expression by real time RT-PCR or immunohistochemistry assay. We wer... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430205 Mon, 21 Nov 2011 08:24:03 +0100 5430205 http://www.medworm.com/index.php?rid=5430204&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076169%26dopt%3DAbstract This study addresses the morphopathogenesis of Achilles tendinosis, using a rat model and presenting quantitative analysis of time-dependent histological changes. Thirty Wistar rats were used, randomly split in experimental and control groups. Animals of the experimental group were submitted to a treadmill running scheme. Five animals of each group were euthanized at four, eight and sixteen weeks. Achilles tendons were collected and processed routinely for histopath sections. Slides were stained by Hematoxylin-Eosin, Picrosirius Red, Alcian Blue, AgNOR, TUNEL and evaluated morphometrically. Cellular density decreased slightly along the time and was higher in the experimental group than in controls at fourth, eighth and sixteenth weeks. Fiber microtearing, percentual of reticular fibers and... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430204 Mon, 21 Nov 2011 08:24:03 +0100 5430204 http://www.medworm.com/index.php?rid=5430203&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076170%26dopt%3DAbstract Authors: Bassiouny AR, Zaky AZ, Abdulmalek SA, Kandeel KM, Ismail A, Moftah M Abstract Oxidative stress in liver cells may contribute to the etiology of hepatic diseases, as in liver cirrhosis. AP-Endonuclease1 (APE1/Ref-1) is essential for cell protection toward oxidative stress by acting as a transcriptional regulator of pro-survival genes and as a redox sensitive protein. The aim of this study was to critically analyze the various parameters governing the success of human umbilical cord blood mononuclear stem cell-based (MNCs) therapy without the use of an immunosuppressant and to investigate for the first time the expression of APE1 during thioacetamide (TAA)-induced cirrhosis and MNCs therapy in a rat model. Umbilical cord blood samples from full-term deliveries were collected... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430203 Mon, 21 Nov 2011 08:24:03 +0100 5430203 http://www.medworm.com/index.php?rid=5430202&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076171%26dopt%3DAbstract Authors: Terada T Abstract Histologic and immunohistochemical features of oncocytic papillary renal cell carcinoma (RCC) have not been fully elucidated. The author herein report a case of oncocytic papillary RCC (OPRCC). A 71-year-old man with diabetes mellitus and diabetic nephropathy was found to have a small right renal tumor by CT. He had been treated with hemodialysis for chronic renal failure for 10 years. A nephrectomy was performed. Grossly, a small (1.5cm) encapsulated yellow tumor was found in the kidney. Histologically, the tumor was completely encapsulated, and consisted entirely of atypical oncocytes arranged in a diffuse papillary structure with fibrovascular cores. The oncocytes showed grade 3 atypia and pseudostratification. A few mitotic figures were seen, and psam... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430202 Mon, 21 Nov 2011 08:24:03 +0100 5430202 http://www.medworm.com/index.php?rid=5430201&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076172%26dopt%3DAbstract We report a patient with extranodal NK/T-cell lymphoma, nasal type that developed extensive bone marrow involvement during the course of her disease with some overlapping features with aggressive NK-cell leukemia. PMID: 22076172 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430201 Mon, 21 Nov 2011 08:24:03 +0100 5430201 http://www.medworm.com/index.php?rid=5430200&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076173%26dopt%3DAbstract Authors: Terada T Abstract The author herein reports a very rare case of pure small cell carcinoma of the esophagus with an emphasis on KIT and PDGFRA. A 72-year-old man was admitted to our hospital because of dysphagia, and endoscopy showed a tumor in the esophagus. A biopsy of the esophageal tumor showed a small cell carcinoma consisting of malignant small cells with very hyperchromatic nuclei and inconspicuous nucleoli and without any differentiations. An immuno-histochemical study revealed positive reaction for cytokeratin (Dako, Glostrup, Denmark), KIT, PDGFRA, synapto-physin, p53 protein, and CD56, and negative reaction for chromogranin, CD45, CD20, CD3, and CD30. The Ki-67 labeling was 95%. A molecular genetic analysis showed no mutations of KIT and PDGFRA genes. The patient... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430200 Mon, 21 Nov 2011 08:24:03 +0100 5430200 http://www.medworm.com/index.php?rid=5430199&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076174%26dopt%3DAbstract Authors: Clement CG, Potluri VR, Gonzales J, Qian YW Abstract Lymphoma is the most frequent malignant tumor of the ocular adnexa with the most common histologic type being extranodal marginal zone B-cell lymphoma (EMZL) of mucosa-associated lymphoid tissue (MALT lymphoma). Here we report a case of a 28 year-old male who presented with a left conjunctival mass of one year duration. A diagnosis of primary MALT lymphoma of the conjunctiva was made based on morphologic and immunopheno-typic studies. Chromosome analysis revealed a male karyotype with a translocation t (5;11) (q33;p11.2) as the primary chromosomal abnormality, which, to the best of our knowledge, is the first reported translocation in MALT lym-phomas and ocular MALT lymphomas as well. PMID: 22076174 [PubMed - in proc... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430199 Mon, 21 Nov 2011 08:24:03 +0100 5430199 http://www.medworm.com/index.php?rid=5430198&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076175%26dopt%3DAbstract Authors: Terada T Abstract A 60-year-old man presented with dysuria and elevated PSA (6.95 ng/ml). Needle biopsies of the prostate revealed well differentiated adenocarcinoma of Gleason's score 6. Prostatectomy and bilateral seminal vesiculotomy were performed. The material was totally cut into 16 preparations. The prostate showed well differentiated adenocarcinoma. The left seminal vesicle showed intraluminal monstrous large epithelial cells with acidophilic cytoplasm and hyperchromatic nuclei, simulating carcinoma cells. Lipochrome pigment was present in the monstrous cells, and some monstrous cells showed large bizarre nuclei. Such monstrous cells were also present in the mucosal seminal vesicle epithelium, and gradual merge between the intraluminal and mucosal monstorous epithe... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430198 Mon, 21 Nov 2011 08:24:03 +0100 5430198 http://www.medworm.com/index.php?rid=5430197&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22076176%26dopt%3DAbstract Authors: Ziats NP, Petersen RB, Lee HG, Castellani RJ, Perry G, Zhu X PMID: 22076176 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5430197 Mon, 21 Nov 2011 08:24:03 +0100 5430197 http://www.medworm.com/index.php?rid=5199210&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904630%26dopt%3DAbstract Authors: Verbeke SLj, Bovée JV Abstract Vascular tumors of bone are a heterogeneous group. Numerous terms have been introduced as well as different classification systems. None of the classification schemes have been accepted due to lack of consistent terminology, accepted histologic criteria, and limited correlation with clinical outcome. It is acknowledged that vascular tumors of bone originate from endothelial cells, resulting in variable expression of endothelial markers. None of these markers are useful to discriminate between benign and malignant lesions. Although radiologic appearance is not specific, radiologic multifocality should trigger to include a vascular neoplasm in the differential diagnosis. This review gives an overview of current literature by describing all dif... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199210 Mon, 15 Aug 2011 04:00:00 +0100 5199210 http://www.medworm.com/index.php?rid=5199209&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904631%26dopt%3DAbstract In this study, we analyzed thirty formalin-fixed paraffin-embedded whole-mount radical prostatectomy specimens of prostate cancer patients. Immunohistochemistry was employed to identify IL-17 producing cells in all sites of mononuclear cell accumulation, noting their relationships to areas of prostate cancer, proliferative inflammatory atrophy (PIA), or hyperplastic benign tissue. Levels of IL-17 producing cells were similar in zones of benign prostate tissue and areas of prostate cancer. Pronounced intraluminal and peri-glandular IL-17 producing cell accumulations were identified in the mononuclear cell infiltrates associated with PIA lesions. Glandular and peri-glandular CD68+ macrophages and neutrophils were the predominant IL-17 producing cells in PIA lesions. The accumulation of IL-17... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199209 Mon, 15 Aug 2011 04:00:00 +0100 5199209 http://www.medworm.com/index.php?rid=5199208&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904632%26dopt%3DAbstract Authors: Ampawong S, Combes V, Hunt NH, Radford J, Chan-Ling T, Pongponratn E, Grau GE Abstract The pathogenic mechanisms underlying the occurrence of cerebral malaria (CM) are still incompletely understood but, clearly, cerebral complications may result from concomitant microvessel obstruction and inflammation. The extent to which brain edema contributes to pathology has not been investigated. Using the model of P. berghei ANKA infection, we compared brain microvessel morphology of CM-susceptible and CM-resistant mice. By quantitative planimetry, we provide evidence that CM is characterized by enlarged perivascular spaces (PVS). We show a dramatic aquaporin 4 (AQP4) upregulation, selectively at the level of astrocytic foot processes, in both CM and non-CM disease, but significantl... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199208 Mon, 15 Aug 2011 04:00:00 +0100 5199208 http://www.medworm.com/index.php?rid=5199207&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904633%26dopt%3DAbstract The objective of this study was to investigate the potential of circulating miRNAs for early detection of lung cancer. We searched the published literature for the miRNA microarray data of primary lung cancer and selected 15 miRNAs that were most frequently up-regulated in lung cancer tissues. Total plasma RNA including miRNAs was isolated, polyade-nylated and reverse-transcribed into cDNAs. The levels of miRNAs were determined by real-time RT-PCR in 74 lung cancer patients and 68 age-matched cancer-free controls. We found that the levels of miR-155, miR-197, and miR-182 in the plasma of lung cancer including stage I patients were significantly elevated compared with controls (P<0.001). The combination of these 3 miRNAs yielded 81.33% sensitivity and 86.76% specificity in discriminating... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199207 Mon, 15 Aug 2011 04:00:00 +0100 5199207 http://www.medworm.com/index.php?rid=5199206&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904634%26dopt%3DAbstract Authors: Iovino SM, Krantz KD, Blanco DM, Fernández JA, Ocampo N, Najafi A, Memarzadeh B, Celeri C, Debabov D, Khosrovi B, Anderson M Abstract Impetigo is a highly contagious bacterial skin infection affecting children worldwide that is caused by the Gram-positive bacteria Staphylococcus aureus, Streptococcus pyogenes, or both. Staphylococcus species can quickly develop drug resistance rendering mupirocin, fusidic acid, and erythromycin ineffective. Preclinical and clinical studies demonstrated that NVC-422 (N, N-dichloro-2, 2-dimethyltaurine) rapidly kills pathogens without the development of drug resistance. 129 patients with clinically diagnosed impetigo were randomized to three dose groups (0.1, 0.5, or 1.5% NVC-422 topical gel) in a study conducted at 2 centers; 125 patients ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199206 Mon, 15 Aug 2011 04:00:00 +0100 5199206 http://www.medworm.com/index.php?rid=5199205&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904635%26dopt%3DAbstract Authors: Al-Hashmi S, Hassan Z, Sadeghi B, Rozell B, Hassan M Abstract Hematopoietic stem cell transplantation (HSCT) is a curative treatment for otherwise incurable diseases. Conditioning regimen is an important part of HSCT and consists of chemotherapy with or without irradiation. Conditioning exerts myelosuppressive, immunosuppressive and antitumor effects, but also contributes to HSCT-related complications including graft-versus-host disease (GVHD). Since almost 50% of the transplanted patients are conditioned with cytostatics without irradiation, we developed and characterized a GVHD mouse model following conditioning with busulphan and cyclophosphamide. Recipient Balb/c female mice were treated with busulphan (20 mg/kg/day for 4 days) and cyclophosphamide (100 mg/kg/day for t... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199205 Mon, 15 Aug 2011 04:00:00 +0100 5199205 http://www.medworm.com/index.php?rid=5199204&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904636%26dopt%3DAbstract CONCLUSION: Aldosterone-receptor blockade by eplerenone effectively attenuated proteinuria, kidney damage and the inflammatory response in anti-GBM GN by significantly decreasing pro-inflammatory cytokines in the regional draining lymph nodes of the kidney. Our results suggest that this selective aldosterone receptor antagonist is a possible additional tool in the treatment of GN. PMID: 21904636 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199204 Mon, 15 Aug 2011 04:00:00 +0100 5199204 http://www.medworm.com/index.php?rid=5199203&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904637%26dopt%3DAbstract The objectives of this study were to determine expression of HIF-1α, angiogenic proteins, angiopoietin-2 (Ang-2), and matrix metalloproteinase 2 (MMP2), and survival/apoptotic proteins (Bcl-xL, caspase 3) in the cerebromicrovasculature of AD transgenic mice and to determine the direct effect of hypoxia on cerebral endothelial expression of these proteins in vitro. Cultured brain endothelial cells were subjected to hypoxia for 4-6 h and analyzed by western blot and immunofluorescence. Our results demonstrated that HIF-1α is induced in cultured brain endothelial cells exposed to hypoxia and that expression of Ang-2, MMP2 and caspase 3 was elevated and the anti-apoptotic protein Bcl-xL decreased. Brain sections from AD and control mice showed that HIF-1α, Ang-2, MMP2 and caspase 3 are elev... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199203 Mon, 15 Aug 2011 04:00:00 +0100 5199203 http://www.medworm.com/index.php?rid=5199202&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904638%26dopt%3DAbstract We present the first reported case of papillary cystadenofibroma of the epididymis. The tumor occurred in a 46-year-old man. The mass was 3.7 cm and included a hemorrhagic fluid-filled cyst. Microscopically, stromal-filled papillae were lined by low cuboidal to columnar epithelium. Epithelial cells were reactive for cytokeratin 7, cy-tokeratins AE1/3, and focally in the apical cytoplasm for CD10. Focal CD10 reactivity was also noted in the stroma. The lesion was negative for alpha-fetoprotein. These findings ruled out other lesions, including metastatic renal cell carcinoma. PMID: 21904638 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199202 Mon, 15 Aug 2011 04:00:00 +0100 5199202 http://www.medworm.com/index.php?rid=5199201&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21904639%26dopt%3DAbstract Authors: Terada T Abstract Immunohistochemical profiles of normal mesothelium and histiocytic/mesothelial hyperplasia (HMH) are unknown. A 19-year-old man was treated by thoracoscopic resection of bullae of left lung. Histologically, there were cell proliferative foci composed of round cells without significant atypia (histiocyte, mesothelium and T-lymphocytes). The cell proliferative foci were patch-like, and no invasive features were seen. Because it is composed of histiocytes, mesothelium, and T-lymphocytes, the diagnosis was HMH. Immunohistochemically, cell components of HMH showed the following immunoreactions: calrenitin 3+, D2-40 3+, pancytokeratin AE1/3 3+, pancytokeratin CAM5.2 3+, cy-tokeratin (CK) 34βE12 1+, CK5/6 1+, CK7 1+, CK8 3+, CK 14 1+, CK18 2+, CK19 2+, p53 10%,... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5199201 Mon, 15 Aug 2011 04:00:00 +0100 5199201 http://www.medworm.com/index.php?rid=5027846&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738815%26dopt%3DAbstract CONCLUSIONS: The results of our study suggest that morphological features such as increased size, ulcer, thrombosis, hemorrhage and presence of aberrant mucosal vessels favor the diagnosis of VM. Co-existence of other clinical conditions such as cardiovascular disease, encountered in association with both lesion types, might exacerbate a tendency towards hemorrhage. PMID: 21738815 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027846 Sun, 19 Jun 2011 23:00:00 +0100 5027846 http://www.medworm.com/index.php?rid=5027845&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738816%26dopt%3DAbstract In conclusion, the proliferation markers Ki-67/MIB-1, PHH3, and survivin are positively correlated with each other and with tumor grade, and may contribute in the identification of aggressive ovarian carcinomas. PMID: 21738816 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027845 Sun, 19 Jun 2011 23:00:00 +0100 5027845 http://www.medworm.com/index.php?rid=5027844&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738818%26dopt%3DAbstract Authors: Barqawi AB, Turcanu R, Gamito EJ, Lucia SM, O'Donnell CI, Crawford ED, La Rosa DD, La Rosa FG Gleason score (GS) (sum of primary plus secondary grades) is used to predict patients' clinical outcome and to customize treatment strategies for prostate cancer (PC). However, due in part to pathologist misreading, there is significant discrepancy of GS between needle-core biopsies (NCB) and radical prostatectomy specimens. We assessed the requirement for re-evaluating NCB diagnosed by outside pathologists in patients referred to our institution for management of PC. In 100 patients, we reviewed both their original "outside" and second-opinion ("in-house") diagnoses of the same NCB specimens, and compared them with the diagnoses of the whole-mount radical prostatectomy (WMRP) specime... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027844 Sun, 19 Jun 2011 23:00:00 +0100 5027844 http://www.medworm.com/index.php?rid=5027843&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738819%26dopt%3DAbstract Authors: Dobashi Y, Watanabe Y, Miwa C, Suzuki S, Koyama S The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates cell growth and metabolism in response to diverse external stimuli. In the presence of mitogenic stimuli, mTOR transduces signals that activate the translational machinery and promote cell growth. mTOR functions as a central node in a complex net of signaling pathways that are involved both in normal physiological, as well as pathogenic events. mTOR signaling occurs in concert with upstream Akt and tuberous sclerosis complex (TSC) and several downstream effectors. During the past few decades, the mTOR-mediated pathway has been shown to promote tumorigenesis through the coordinated phosphorylation of proteins that directly regulate cell-cycle pr... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027843 Sun, 19 Jun 2011 23:00:00 +0100 5027843 http://www.medworm.com/index.php?rid=5027842&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738820%26dopt%3DAbstract Authors: Zhang J, Yu Z, Yu Z, Yang Z, Zhao H, Liu L, Zhao J Stroke is one of the neurological diseases which lead to permanently neuronal damage after temporary or long-term occlusion of vessels or after heart attack. However, there are few efficient strategies to prevent or treat this kind of insult in clinical because the consequence is irreversible and could be long-lasting after the onset of stroke. Gene therapy especially using viral system has long been addressed to be of great potential to reduce the damage. Here, we generated recombinant adeno-associated virus (rAAV) carrying brain-derived neurotrophic factor (BDNF) gene. Cells infected with rAAV-BDNF could be able to produce functional BDNF which promoted neurite outgrowth and protected neurons from apoptosis induced by serum ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027842 Sun, 19 Jun 2011 23:00:00 +0100 5027842 http://www.medworm.com/index.php?rid=5027841&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738821%26dopt%3DAbstract In conclusion, the global normalization method helped to define positive p53 staining in the tissue microarray set used. The method used helped to define clear cut-off points and confirmed all negatively stained tissue cores. Such normalization methods should help to better define clinically useful biomarkers. PMID: 21738821 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027841 Sun, 19 Jun 2011 23:00:00 +0100 5027841 http://www.medworm.com/index.php?rid=5027840&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738822%26dopt%3DAbstract Authors: Zhao G, Zhang ZQ, Zhang B, Luo M, Sun YW, Wu ZY Expressed in hepatic stellate cell (HSC), tTG is involved in fibrotic diseases including human hepatic fibrosis by promoting the cross-linking of ECM and participating in the initiation and/or progression of liver fibrosis. The purpose of this study is to identify whether depletion of tTG could attenuate liver fibrosis. PMID: 21738822 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027840 Sun, 19 Jun 2011 23:00:00 +0100 5027840 http://www.medworm.com/index.php?rid=5027839&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738823%26dopt%3DAbstract In conclusion, our data demonstrate that cardiac hypertrophy can be induced in an E2F1-independent fashion and suggest that in contrast to previous reports, targeting E2F1 may not be a good therapeutic approach. PMID: 21738823 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027839 Sun, 19 Jun 2011 23:00:00 +0100 5027839 http://www.medworm.com/index.php?rid=5027838&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738824%26dopt%3DAbstract CONCLUSIONS: We conclude that this disorder may have several components in its etiopathology, including a possible restricted immune response and a possible genetic component; these possibilities warrant further investigation. PMID: 21738824 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027838 Sun, 19 Jun 2011 23:00:00 +0100 5027838 http://www.medworm.com/index.php?rid=5027837&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21738825%26dopt%3DAbstract Authors: Savage NM, Alleyne CH, Vender JR, Figueroa R, Zhang H, Samuel TA, Sharma S Clinical presentation with dural-based metastasis mimicking meningiomas is rare. We aimed to evaluate the role of frozen section in guiding surgery and histopathologic diagnosis in determining primary sites of dural-based metastatic carcinomas. Following the receipt of HAC approval, we retrospectively reviewed 7cases presenting with dural-based masses clinically suspected to be primary brain tumors (6 meningiomas and 1 superficial glioblastoma), but diagnosed to be metastatic carcinomas on subsequent resection. Pertinent clinical records and follow-up data were reviewed. Patient's age ranged from 59 to 80 years. Imaging showed extra-axial dural-based masses with contiguous but not primary brain involvem... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=5027837 Sun, 19 Jun 2011 23:00:00 +0100 5027837 http://www.medworm.com/index.php?rid=4884550&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577318%26dopt%3DAbstract In conclusion, our results point to rare evidence of Lgr5 positive stem cell like cells in the metastatic cascade of colorectal cancer, but these few cells might be biologically powerful in the metastatic process of cancer subsets. Clonal analysis is necessary to proof this hypothesis. PMID: 21577318 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884550 Thu, 31 Mar 2011 23:00:00 +0100 4884550 http://www.medworm.com/index.php?rid=4884549&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577319%26dopt%3DAbstract Authors: Schwarz S, Müller M, Ettl T, Stockmann P, Zenk J, Agaimy A We analyzed 41 oral salivary gland carcinomas from consecutive 290 salivary gland carcinoma database (14%) with emphasis on the histological spectrum and clinical outcome of adenoid cystic carcinoma (ACC) and polymorphous low-grade adenocarcinoma (PLGA). The cohort included 14 ACCs, 14 mucoepidermoid carcinomas (MECs), 8 PLGAs, 3 adenocarcinomas, not otherwise specified and 2 acinic cell carcinomas. Mean age was 48, 58 and 61 yrs for ACC, MEC and PLGA, respectively. Eight patients (19.5%) died of tumor at a mean interval of 66.5 months. ACC and PLGA showed similar mean age, gender distribution, predominant palatal localization, nodal metastasis, perineural invasion and MIB-1 index. However, ACC tended to show higher t... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884549 Thu, 31 Mar 2011 23:00:00 +0100 4884549 http://www.medworm.com/index.php?rid=4884548&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577320%26dopt%3DAbstract Authors: Kliment CR, Englert JM, Crum LP, Oury TD The purpose of this study was to develop an improved method for collagen and protein assessment of fibrotic lungs while decreasing animal use. PMID: 21577320 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884548 Thu, 31 Mar 2011 23:00:00 +0100 4884548 http://www.medworm.com/index.php?rid=4884547&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577321%26dopt%3DAbstract This study is the first to suggest a potential role for activated pSTAT3 in lymphatic metastases in thyroid cancer. PMID: 21577321 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884547 Thu, 31 Mar 2011 23:00:00 +0100 4884547 http://www.medworm.com/index.php?rid=4884546&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577322%26dopt%3DAbstract This study aimed to evaluate the association between the immunohistochemical expression of NAD(P) H:quinone oxidoreductase-1 (NQO1) and nuclear factor erythroid 2-related factor 2 (Nrf2) in resected specimens of intrahepatic cholangiocarcinoma (ICC) and to elucidate the prognostic value of NQO1 and Nrf2 expression. A retrospective analysis was conducted of 34 consecutive patients who underwent surgical resection for ICC. Immunohistochemistry of the resected specimens was conducted using each of the following primary monoclonal antibodies against NQO1 and Nrf2. Of the 34 patients, 23 were classified as having tumors with NQO1-positive expression and 11 had tumors with loss of NQO1 expression, whereas 22 patients had tumors with Nrf2-positive expression and 12 had tumors with loss of Nrf2 ex... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884546 Thu, 31 Mar 2011 23:00:00 +0100 4884546 http://www.medworm.com/index.php?rid=4884545&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577323%26dopt%3DAbstract We describe the clinicopathologic features of 18 AML patients with isolated +11 and their mutation status of NPM1, FLT3, NRAS ,KRAS, and KIT. Fourteen patients had de novo AML and 4 patients had a history of myelodysplastic syndrome (MDS). Fifteen patients had a progressive clinical course with refractory or relapsed disease. The median overall survival was 5 months (range, 2 to 48 months). Only 1 patient achieved complete remission after undergoing stem cell transplantation. The bone marrow median blast count was 65% (range, 22 to 86) and 14 patients had blasts >50%. The most common type of AML was AML without maturation (7 patients) classified by the World Health Organization classification system, or M1 (10 patients) by the French-American-British (FAB) system. FLT3 mutations were de... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884545 Thu, 31 Mar 2011 23:00:00 +0100 4884545 http://www.medworm.com/index.php?rid=4884544&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577324%26dopt%3DAbstract Authors: Suzuki Y, Yazawa I Dentatorubral-pallidoluysian atrophy (DRPLA) is caused by the expansion of polyglutamine (polyQ) in atrophin-1 (ATN1), also known as DRPLA protein. ATN1 is ubiquitously expressed in the central nervous system (CNS), although selective regions of CNS are degenerated in DRPLA, and this selective neuronal damage gives rise to the specific clinical features of DRPLA. Accumulation of mutant ATN1 that carries an expanded polyQ tract seems to be the primary cause of DRPLA neurodegeneration, but it is still unclear how the accumulation of ATN1 leads to neu-rodegeneration. Recently, cleaved fragments of ATN1 were shown to accumulate in the disease models and the brain tissues of patients with DRPLA. Furthermore, proteolytic processing of ATN1 may regulate the intrace... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884544 Thu, 31 Mar 2011 23:00:00 +0100 4884544 http://www.medworm.com/index.php?rid=4884543&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577325%26dopt%3DAbstract Authors: Yen SS The proteasomal degradation of cytosolic, phosphorylation-independent tau in human brains is potentially linked to the pathogenesis of neurofibrillary pathology in Alzheimer's disease (AD). Previous studies showed that the active 20S proteasome core degrades recombinant tau effectively, which prompted this study to determine if there was evidence of proteasomal degradation of tau in human brain with a range of neurofibrillary pathology. Cytosolic proteins from temporal cortex were isolated from 30,000xgsupernatants by resolving in size-exclusion chromatogra-phy for assay of tau and proteasomal subunits by Western blots. Levels of tau and proteasome subunits varied from case to case, with a significant inverse correlation between the levels of tau and 20S β-subunits, an... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884543 Thu, 31 Mar 2011 23:00:00 +0100 4884543 http://www.medworm.com/index.php?rid=4884542&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577326%26dopt%3DAbstract We present a case of de novo non-keratinizing carcinoma of the nasopharynx (NK-NPC) with an unusual combination of histological features; (1) a minimal associated component of reactive lymphoplasmacytic cells and (2) a prominent desmoplastic stromal response. Apart from the unusual histologic features, this case did not display any unusual clinical or radiological features. On immunohistochemistry the tumor cells were strongly positive for cy-tokeratins (AE1-3 and 5/6) and p63 and there was strong and diffuse nuclear positivity for EBV on in situ hybridization. Since no external factor could be attributed to the conspicuous paucity of associated lymphoid cells, we feel that this may be due to inherent features of the neoplasm itself. This case highlights the histomorphological variability ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884542 Thu, 31 Mar 2011 23:00:00 +0100 4884542 http://www.medworm.com/index.php?rid=4884541&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577327%26dopt%3DAbstract Authors: Ahsanuddin AN, Brynes RK, Li S Angioimmunoblastic T-cell lymphoma (AITL) is a unique type of peripheral T-cell lymphoma. Patients with AITL may have occasional reactive plasma cells present in the peripheral circulation. Prominent peripheral blood polyclonal plasmacytosis mimicking plasma cell leukemia, however, is distinctly uncommon. Here we describe 3 such cases from two large tertiary medical centers and discuss the role of ancillary studies in the differential diagnosis of peripheral blood plasmacytosis. PMID: 21577327 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884541 Thu, 31 Mar 2011 23:00:00 +0100 4884541 http://www.medworm.com/index.php?rid=4884540&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21577328%26dopt%3DAbstract We describe the clinicopathologic and immunohistochemical features of a multifocal hepatic EBV-SMT in a 34-year-old acquired immunodeficiency syndrome (AIDS) patient and investigate the activation status of the mTOR signal pathway in this tumor. In addition, we provide a review of the literature on the clinicopathologic findings of hepatic EBV-SMT in adult AIDS patients, and discuss their biologies and possible therapeutic strategies. PMID: 21577328 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884540 Thu, 31 Mar 2011 23:00:00 +0100 4884540 http://www.medworm.com/index.php?rid=4884539&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21594003%26dopt%3DAbstract Authors: Gotoh N Non-small cell lung cancer (NSCLC) is a major subtype of lung cancer that has been the most common and most fatal cancer worldwide. Gefitinib (Iressaâ„¢) and erlotinib (Tarcevaâ„¢), specific tyrosine kinase inhibitors (TKI) for the epidermal growth factor receptor (EGFR), have been demonstrated to be effective for some NSCLC patients and are pioneering molecular-targeted drugs used in the clinic for cancer. Because many studies indicate that only some patient populations benefit from these drugs, there has been an urgent need to develop diagnostic methods to select appropriate patients for whom treatment with these drugs will be beneficial. Moreover, problems of acquired resistance after long-term treatment with the drugs have emerged. In this review, I summarize the c... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4884539 Thu, 31 Mar 2011 23:00:00 +0100 4884539 http://www.medworm.com/index.php?rid=4727030&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487518%26dopt%3DAbstract Authors: Patel VP, Chu CT Trafficking of transcription factors between the cytoplasm and the nucleus is an essential aspect of signal transduction, which is particularly challenging in neurons due to their highly polarized structure. Disruption in the subcellular localization of many proteins, including transcription factors, is observed in affected neurons of human neurodegenerative diseases. In these diseases, there is also growing evidence supporting alterations in nuclear transport as potential mechanisms underlying the observed mislocalization of proteins. Oxidative stress, which plays a key pathogenic role in these diseases, has also been associated with significant alterations in nuclear transport. After providing an overview of the major nuclear import and export pathways and d... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727030 Wed, 30 Mar 2011 23:00:00 +0100 4727030 http://www.medworm.com/index.php?rid=4727028&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487519%26dopt%3DAbstract Authors: Uozaki H, Barua RR, Minhua S, Ushiku T, Hino R, Shinozaki A, Sakatani T, Fukayama M Gastric cancer (GC) is a major cancer, sometimes associated with Epstein-Barr virus (EBV). Some transcriptional factors (TFs) are specific to the digestive tract and related to the character of the tumors. PMID: 21487519 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727028 Wed, 30 Mar 2011 23:00:00 +0100 4727028 http://www.medworm.com/index.php?rid=4727021&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487520%26dopt%3DAbstract This study confirms paradoxical responses to two different models of pulmonary fibrosis and suggests a further role for RAGE in cellular migration. PMID: 21487520 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727021 Wed, 30 Mar 2011 23:00:00 +0100 4727021 http://www.medworm.com/index.php?rid=4727016&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487521%26dopt%3DAbstract Authors: Matsunami T, Sato Y, Hasegawa Y, Ariga S, Kashimura H, Sato T, Yukawa M An important source of reactive oxygen species (ROS) production is nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which on activation induces superoxide production via oxidation in the mitochondria, inflammation and stress; such ROS are implicated in the pathogenesis of diabetic complications, including neuropathy. Hyperbaric oxygen (HBO) treatments are applied various diseases including diabetic patients with unhealing foot ulcers, however, and also increases the formation of ROS. In a previous study, we showed that a clinically recommended HBO treatment significantly enhanced oxidative stress of pancreatic tissue in the diabetic rats. However, no study has been undertaken with regard to the... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727016 Wed, 30 Mar 2011 23:00:00 +0100 4727016 http://www.medworm.com/index.php?rid=4727013&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487522%26dopt%3DAbstract Authors: Soini Y, Haapasaari KM, Vaarala MH, Turpeenniemi-Hujanen T, Kärjä V, Karihtala P Oxidative stress markers and peroxiredoxins are connected to cancer. A large set of urinary bladder carcinomas were studied for the expression of nitrotyrosine and 8-hydroxydeguanosine (8OHdG) , two markers indicating oxidative damage. Serum and urine 8-OHdG were assessed in a subset of patients. We also analysed immunohisto-chemically the expression of nrf2, keap1, all six peroxiredoxins (prx) and thioredoxin (trx) in these tumors. 15 % of the cases showed 8OHdG and 36 % nitrotyrosine positivity. Expression of nitrotyrosine and 8OHdG associated with a poor prognosis (p=0.050, p=0.011, respectively). Peroxiredoxin positivity ranged from 39 % to 84 % lowest expression being for prx 4 and highest ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727013 Wed, 30 Mar 2011 23:00:00 +0100 4727013 http://www.medworm.com/index.php?rid=4727006&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487523%26dopt%3DAbstract Authors: Li MQ, Tang CL, Du MR, Fan DX, Zhao HB, Xu B, Li DJ Tetraspanin CD82 has been identified as a potential contributor to controlling trophoblast invasiveness in human first-trimester pregnancy. However, it is unclear how the regulation of CD82 expression at maternal-fetal interface. The present study is to investigate the effect of the trophoblast-derived CXCL12 on CD82 expression in decidual stromal cells (DSCs) that in turn controls trophoblast cell invasiveness. In-cell Western was used to evaluate the expression of CD82 in DSCs. A co-culture model was established to investigate the reciprocal interaction between trophoblasts and DSCs via CXCL12/CXCR4 and CD82 expression. We found that both anti-CXCL12 and anti-CXCR4 neutralizing antibody can eliminate increase of CD82 expres... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727006 Wed, 30 Mar 2011 23:00:00 +0100 4727006 http://www.medworm.com/index.php?rid=4727002&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487524%26dopt%3DAbstract In conclusion, our data suggest that IL-15 may contribute to atherosclerotic plaque integrity by stimulation of smooth muscle cells, probably in a PDGF dependent fashion. PMID: 21487524 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4727002 Wed, 30 Mar 2011 23:00:00 +0100 4727002 http://www.medworm.com/index.php?rid=4726995&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487525%26dopt%3DAbstract Authors: Graddis TJ, McMahan CJ, Tamman J, Page KJ, Trager JB Prostate cancer is the most common cancer and the second leading cause of cancer deaths among males in most Western countries. Autologous cellular immunotherapy for the treatment of cancer seeks to induce tumor-specific immunity in the patient and is consequently dependent on a suitable target antigen and effective presentation of that antigen to the patient's immune system. Prostatic acid phosphatase (PAP) has been tested as a target antigen due to its high and apparently specific expression in the prostate. We used a variety of approaches to analyze PAP expression, including immunohistochemistry, in situ hybridization, and quantitative polymerase chain reaction. We complemented these laboratory-based techniques with an in ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4726995 Wed, 30 Mar 2011 23:00:00 +0100 4726995 http://www.medworm.com/index.php?rid=4726990&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487526%26dopt%3DAbstract We describe the case of a 77-year-old man who presented with slightly progressive chest pain associated with dyspnea, fatigue and edema of the lungs. Transthoracic echocardiography detected a left atrial mass that has been successfully excised. Histopathological examination showed a neoplasm combining features of both atrial myxoma and PFE. However, close evaluation of the latter showed microscopic foci of myxomatous tissue within papillary cores, indicating that the PFE-like component has developed around preexisting myxomatous tissue that served as a nidus for papillary fronds, probably by a process of fibrinous microthrombosis, organization and endothelialisation. This unusual case may shed light on the pathogenesis of the PFE pattern. PMID: 21487526 [PubMed - in process] (Source: I... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4726990 Wed, 30 Mar 2011 23:00:00 +0100 4726990 http://www.medworm.com/index.php?rid=4726982&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487527%26dopt%3DAbstract Authors: Gui L, Chen K, Zhang Y H1N1 vaccination is currently safe, and only rare acceptable side-effects have been reported. Here we describe for the first time a serious adverse event, i.e., acute transverse myelitis, following H1N1 vaccination in China. After the standard treatment with methylprednislone, the patient recovered completely. PMID: 21487527 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4726982 Wed, 30 Mar 2011 23:00:00 +0100 4726982 http://www.medworm.com/index.php?rid=4726969&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487528%26dopt%3DAbstract Authors: Lee L, Zhou F, Simms A, Wieczorek R, Fang Y, Subietas-Mayol A, Wang B, Heller P, Huang H, Pei Z, Osman I, Meehan S, Lee P A case of metastatic balloon cell malignant melanoma (BCMM) is presented. The balloon melanoma cells (BMC) were absent in the shave biopsy of the primary lesion and present as a minor component in the wide and deep excision. A subsequent right neck lymph node metastasis showed complete replacement of the lymph node by large, foamy cells. Though the tumor was amelanocytic and Fontana-Masson stain failed to reveal melanin, it stained positively for S-100, HMB-45, and Melan-A. Ultrastructurally, the foamy cells were characterized by cytoplasmic vacuoliza-tion and a lack of melanosomes. The differential diagnosis of metastatic balloon cell malignant melanoma is... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4726969 Wed, 30 Mar 2011 23:00:00 +0100 4726969 http://www.medworm.com/index.php?rid=4726963&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487529%26dopt%3DAbstract We present two cases of 5q- syndrome with a similar and unusual course of transformation to lymphoblastic leukemia while on Lenalidomide. These two patients achieved an initial response; however, later acquired a second cytogenetic abnormality, became refractory to treatment and evolved into acute leukemia. At the time of transformation, both patients had recurrence of the 5q- abnormality. Review of the literature and the mechanisms of transformation of the 5q-syndrome into an acute leukemia are discussed. Although the relationship between the events in our cases remains unclear, the intriguing similarity between the two cases raises a question whether immune modulators can alter the natural course of MDS. To our knowledge, no similar cases were previously reported in the literature. P... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4726963 Wed, 30 Mar 2011 23:00:00 +0100 4726963 http://www.medworm.com/index.php?rid=4498639&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326808%26dopt%3DAbstract In conclusion, our study makes the novel observation that signalling through NPM-ALK and JunB affect the expression of cytotoxic molecules in ALK+ ALCL. Moreover, these findings demonstrate the expression of GzB and Perforin in this lymphoma is not solely due its presumed CTL origin, but that oncogenic signalling is actively influencing the expression of these proteins. PMID: 21326808 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498639 Sun, 20 Feb 2011 16:45:03 +0100 4498639 http://www.medworm.com/index.php?rid=4498638&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326809%26dopt%3DAbstract Authors: Wilson AC, Dugger BN, Dickson DW, Wang DS Transactive response DNA-binding protein of 43 kDa (TDP-43), an RNA and DNA binding protein involved in transcriptional repression, RNA splicing and RNA metabolism during the stress response, is the major component of neuronal inclusions in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin inclusions, now referred to as FTLD-TDP. While initially thought to be relatively specific to ALS and FTLD-TDP, TDP-43 pathology has now been detected in a number of other neurodegenerative diseases, many associated with tau pathology, including Guam Parkinson dementia complex and Alzheimer's disease (AD). TDP-43 pathology is detected in 25% to 50% of AD cases, especially those with more severe clinical phenotyp... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498638 Sun, 20 Feb 2011 16:45:03 +0100 4498638 http://www.medworm.com/index.php?rid=4498637&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326810%26dopt%3DAbstract Authors: Ferruelo A, El-Assar M, Lorente JA, Nin N, Peñuelas O, Fernández-Segoviano P, Gonzalez C, Esteban A Samples used for genotyping and transcription studies are obtained and conserved in very specific conditions. The possibility to use autopsy tissue samples, which contain nucleic acids of very poor quality, would open new possibilities for genetic studies. PMID: 21326810 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498637 Sun, 20 Feb 2011 16:45:03 +0100 4498637 http://www.medworm.com/index.php?rid=4498636&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326811%26dopt%3DAbstract Authors: Zheng J, Xu DF, Li K, Wang HT, Shen PC, Lin M, Cao XH, Wang R Some women in childbearing ages take selective serotonin reuptake inhibitors (SSRIs) such as fluoxetine and fluvoxamine for treating psychiatric symptoms. However, these compounds may cause some side effects to their children. It has been identified that early life exposure to SSRIs increased the chance of developing mood disorders and the biological basis is still unclear. Here, we studied the effects of neonatal exposure to SSRIs on neuronal morphology. We used GFP-transgenic mice to investigate the acute and long-lasting effects of early life exposure to SSRIs on dendritic spine density of CA1 neurons. We found that 18-day drug applications of fluoxetine and fluvoxamine significantly reduced spine density of basa... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498636 Sun, 20 Feb 2011 16:45:03 +0100 4498636 http://www.medworm.com/index.php?rid=4498635&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326812%26dopt%3DAbstract This study explores the relevance of two nuclear parameters in osteosarcoma: large area and round shape. Computerized nuclear morphometry was performed in 56 conventional osteosarcoma preoperative biopsies. The mean patient follow-up time was 35.1 months. Based on the nuclear area, no significant difference (P = 0.09) in overall survival between patients with large (> 42.5 μm(2)) and small (< 42.5 μm(2)) tumor nuclei was found. However, when cases with large and round nuclei were analyzed jointly (> 42.5 μm(2) and coefficient of nuclear roundness > 0.7), these two parameters together were likely to be a predictive factor (P = 0.05). Osteosarcoma patients with large and round tumor nuclei had a better outcome than patients with small and polymorphic (ovoid or spindle-shaped) ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498635 Sun, 20 Feb 2011 16:45:03 +0100 4498635 http://www.medworm.com/index.php?rid=4498634&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326813%26dopt%3DAbstract In conclusion, D2-40 immunohistochemistry reliably identifies the MEC of breast in a variety of lesions in a pattern similar to that of calponin and p63, and can be used as an additional MEC marker. Caution should be exercised when interpreting the staining of cells surrounding DCIS and carcinoma with retraction artifact. PMID: 21326813 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498634 Sun, 20 Feb 2011 16:45:03 +0100 4498634 http://www.medworm.com/index.php?rid=4498633&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326814%26dopt%3DAbstract We present the case of a 67-year-old Taiwanese male with HCL-v who had leukocytosis and splenomegaly. His hairy leukemic cells were weakly positive for TRAP and expressed CDllc and CD103 but not CD25. He received oral chemotherapy with chlorambucil and in complete hematological remission in 9 months but relapsed 2 months later. Literature review revealed 9 cases of HCL and 3 cases of HCL-v including current case from Taiwan. All patients were adults with splenomegaly. The HCL patients had a significantly higher frequency of leukopenia (p = 0.024) and monocytopenia (p = 0.008) and a lower frequency of leukocytosis (p = 0.018) than HCL-v patients. All 8 HCL patients responded favorably to 2-chlorodeoxyadenosine with or without splenectomy. The 3 HCL-v patients had leukocytosis and received c... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498633 Sun, 20 Feb 2011 16:45:03 +0100 4498633 http://www.medworm.com/index.php?rid=4498632&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326815%26dopt%3DAbstract We describe a granular cell tumor arising from the tissue adjacent to the thyroid gland and with multiple organ/tissue involvement in a 27-year-old woman with an initial presentation of a thyroid mass. The diagnosis was made by fine needle aspiration and immunocytochemial stains in conjunction with radiological examination. To our knowledge, there is no previous report of such cases in the literature and the importance of fine needle aspiration in conjunction with radiologic examination in the evaluation of granular cell tumor presenting as a thyroid mass has not been well addressed. PMID: 21326815 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498632 Sun, 20 Feb 2011 16:45:03 +0100 4498632 http://www.medworm.com/index.php?rid=4498631&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326816%26dopt%3DAbstract We present here a case of invasive variant of pleomoprhic lobular carcinoma with coexisting classic and pleomorphic variants of lobular carcinoma in situ along with focal ductal carcinoma in situ. The immunohistochemical results on hormone receptors and high molecular weight cytokeratins in all the above components of the tumor are presented. The invasive tumor was negative for estrogen receptors, progesterone receptors and Her2/neu. Most foci of lobular carcinoma in situ showed morphogenic heterogeneity and a corresponding heterogenous staining for hormone receptors. The high molecular weight cytokeratins (CK5/6 and CK 903) were non contributory in establishing diagnosis. PMID: 21326816 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498631 Sun, 20 Feb 2011 16:45:03 +0100 4498631 http://www.medworm.com/index.php?rid=4498630&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326817%26dopt%3DAbstract We report an unusual case of urethral carcinosarcoma from a female patient with melanocytic differentiation. The tumor consists of a high-grade papillary serous carcinoma with psammoma bodies and a mesenchymal component with area of heterologous (cartilaginous) element. More interestingly, there are epithelioid tumor cells containing melanin pigment. On immunohistochemical stains, the epithelioid tumor cells are positive for S100, HMB45 and Mart-1, but negative for cytokeratin. This case represents an unusual carcinosarcoma with areas of melanocytic differentiation. Such rare tumors have been occasionally reported in the breast, uterus, kidney, and lung. These cases demonstrate the capacity of tumor cells to differentiate into divergent elements, supporting the concept of pluripotent tumor... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498630 Sun, 20 Feb 2011 16:45:03 +0100 4498630 http://www.medworm.com/index.php?rid=4498629&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21326818%26dopt%3DAbstract Authors: Demir R, Schmid A, Hohenberger W, Agaimy A Primary mesenteric tumors are exceedingly rare and may thus pose a diagnostic challenge. They encompass both benign and malignant neoplasms as well as reactive and idiopathic tumefactive fibroinflammatory lesions. PMID: 21326818 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4498629 Sun, 20 Feb 2011 16:45:03 +0100 4498629 http://www.medworm.com/index.php?rid=4288940&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151387%26dopt%3DAbstract In this report, we describe a widespread expression of AKR1C3 in glial neoplasms and meningiomas, with limited expression in medulloblastoma and no expression in Schwannoma. These tumors, except meningioma, are not classically considered to be sex hormone-dependent or related brain tumors. The current results corroborate our earlier observations that AKR1C3 is expressed in both sex hormone-dependent and hormone-independent malignancies. Similar to AKR1C3 distribution in Wilm's tumor, we also demonstrate that expression of AKR1C3 is reduced in tumors with embryonic phenotypes. PMID: 21151387 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288940 Sun, 26 Dec 2010 11:20:02 +0100 4288940 http://www.medworm.com/index.php?rid=4288939&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151388%26dopt%3DAbstract In this study, we retrieved 2 thyroidectomy specimens of ATC with coexisting well differentiated thyroid carcinomas (DTCs) including one papillary carcinoma (PTC) and one follicular carcinoma (FTC). We used im-munohistochemistry to examine the expression of stem cell markers (nestin, CD133 and CD44) and a marker for EMT (E-cadherin). Intense expressions of nestin, CD133 and CD44, and no expression of E-cadherin were observed in both ATCs. In contrast, the PTC and FTC, and non-neoplastic thyroid tissue in both cases were negative for nestin and positive for E-cadherin. The expressions of CD133 and CD44 were variable in the PTC, FTC, and non-neoplastic thyroid tissue and were at a lower level of expression of these markers in the overall pattern. The results confirmed EMT, demonstrated the s... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288939 Sun, 26 Dec 2010 11:20:02 +0100 4288939 http://www.medworm.com/index.php?rid=4288938&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151389%26dopt%3DAbstract Authors: Jiang L, Marlow LA, Cooper SJ, Roemeling CV, Menke DM, Copland JA, Tun HW Primary Central nervous system lymphoma (PCNSL) is most frequently a diffuse large B cell lymphoma (DLBCL), which is confined to the Central nervous system (CNS). We performed an experiment in which lymphoma cells from a PCNSL patient were implanted subcutaneously in an athymic mouse. The lymphoma cells were shown to home to the CNS with histologic evaluations of the brain showing multiple large B cells in blood vessels consistent with intravascular large B cell lymphoma (IVL). We did not find any evidence of lymphoma at the site of implantation or other locations. The findings are consistent with highly selective tropism of PCNSLforthe CNS and its vasculature. PMID: 21151389 [PubMed - in process] (S... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288938 Sun, 26 Dec 2010 11:20:02 +0100 4288938 http://www.medworm.com/index.php?rid=4288937&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151390%26dopt%3DAbstract We report the correlation of Mcl-1 protein expression with higher grade and stage in colorectal cancer. Mcl-1 correlated also with pAKT expression. We also report the up regulation of pAKT during the transition from NR to CRC. PMID: 21151390 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288937 Sun, 26 Dec 2010 11:20:02 +0100 4288937 http://www.medworm.com/index.php?rid=4288936&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151391%26dopt%3DAbstract This study investigates the relationship between MMP-2 immunoexpression and angiogenesis in a series of lung carcinomas metastatic to the central nervous system (CNS). Twenty eight metastatic carcinoma cases with adequate brain-tumor interface were identified from the archives at the Moffitt Cancer Center. MMP-2 expression was determined by immunohistochemistry using an antibody directed against pro and active forms (NeoMarkers). Similarly, microvessels were identified on parallel sections with anti-CD34 antibody (Biogenix). Angiogenesis profiles within the tumor and at the CNS/tumor interface were morphometrically assessed by the Image Pro Plus image analysis system. Briefly, CD34 positive vessels were quantitated and correlated with presence or absence of MMP-2 expression in the tumor. M... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288936 Sun, 26 Dec 2010 11:20:02 +0100 4288936 http://www.medworm.com/index.php?rid=4288935&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151392%26dopt%3DAbstract CONCLUSIONS: Beta-catenin nuclear location could be considered as a paraneoplastic pattern which is considerably tumor-related. ERas may be a potential biomarker for gastric cancer, but advanced studies are wanted. GKN2 reduction is indiscernible by immunostaining. PMID: 21151392 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288935 Sun, 26 Dec 2010 11:20:02 +0100 4288935 http://www.medworm.com/index.php?rid=4288934&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151393%26dopt%3DAbstract Authors: Dhingra S, Li W, Tan D, Zenali M, Zhang H, Brown RE Fibrolamellar hepatocellular carcinoma (FLHCC) has a better prognosis than conventional hepatocellular carcinoma. Nevertheless, FLHCC has a propensity to recur with limited responsiveness to chemotherapy. PMID: 21151393 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288934 Sun, 26 Dec 2010 11:20:02 +0100 4288934 http://www.medworm.com/index.php?rid=4288933&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151394%26dopt%3DAbstract We report a CD20dim- positive T-cell large granular lymphocytic (T-LGL) leukemia in a patient with concurrent hairy cell leukemia and plasma cell myeloma. This patient was first diagnosed with T-LGL leukemia with dim CD20 expression, which by itself was a rare entity. He received no treatment for T-LGL leukemia. The patient later developed a hairy cell leukemia, which went into complete clinical remission after one cycle of 2-CdA. Five years later, he was diagnosed with a third malignancy, plasma cell myeloma. Complex cytogenetic aberrancies were present at the time when plasma cell myeloma was diagnosed. This is the first report, to the best of our knowledge, in the English literature with the aforementioned three distinct hematopoietic malignancies in one patient. PMID: 21151394 [Pub... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288933 Sun, 26 Dec 2010 11:20:02 +0100 4288933 http://www.medworm.com/index.php?rid=4288932&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151395%26dopt%3DAbstract This report demonstrated that the findings of malignant spindle cell proliferation does not imply this entity to be carcinosarcoma. The distinction of AC from true sarcomas is important because of the poorer prognosis of the later compared with the quite favorable behavior of AC. However, such existence necessitates a careful tissue sampling for the logical distinction between AC and carcinosarcoma, which is critical for planning further management and ultimately the predictor of prognosis. PMID: 21151395 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288932 Sun, 26 Dec 2010 11:20:02 +0100 4288932 http://www.medworm.com/index.php?rid=4288931&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151396%26dopt%3DAbstract We report the case of a 49 year old male with a history of NFI and MPNST involving only the right elbow which was excised with clear margins. Five years later, he presented with an isolated 11.5 × 9.0 × 7.0 centimeter right axillary mass which was completely excised. Microscopic examination revealed metastatic MPNST with lymph node involvement. Although isolated lymph node metastasis from MPNST is rare, this case expands our knowledge of the clinical behavior of MPNST. PMID: 21151396 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288931 Sun, 26 Dec 2010 11:20:02 +0100 4288931 http://www.medworm.com/index.php?rid=4288930&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151397%26dopt%3DAbstract Authors: Haycocks NG, Zhao XF Large B-cell lymphoma presents with the most varied infiltrating patterns and morphologies. Here we report two cases of unusual large B-cell lymphoma in two old female patients. Both lymphomas show: 1) scattered and clustered large B-cells infiltrating the periphery of polyclonal lymphoid nodules; 2) large B-cells with an immunoblastic morphology; 3) large B-cell infiltration associated with vascular proliferation; 4) coexisting lymphoid nodules with hyaline vascular proliferation. The first case took an aggressive clinical course with transformation into acute leukemia, and imparted a short patient survival, whereas the second case responded to chemotherapy, experienced a local recurrence and long survival for >7 years. To our knowledge, these are the ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288930 Sun, 26 Dec 2010 11:20:02 +0100 4288930 http://www.medworm.com/index.php?rid=4288929&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21151398%26dopt%3DAbstract Authors: Xianfeng F. Zhao. Pitfalls in Diagnostic Hematopathology: Part I. Int J Clin Exp Pathol. 2009;2(1):11-20.On page 12, in the sentence interpreting the data of Table 2, the median survival months of CLL/SLL memory B-cells (293 months) and näive B-cells (109 months) were mistakenly switched. However the data in Table 2 is correct. The conclusion is not adversely affected by this error.[This corrects the article on p. 11 in vol. 2, PMID: 18830390.]. PMID: 21151398 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=4288929 Sun, 26 Dec 2010 11:20:02 +0100 4288929 http://www.medworm.com/index.php?rid=3959834&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830235%26dopt%3DAbstract Authors: Rodero MP, Khosrotehrani K Skin wound healing is a multi stage phenomenon that requires the activation, recruitment or activity of numerous cell types as keratinocytes, endothelial cells, fibroblast and inflammatory cells. Among the latter, macrophages appear to be central to this process. They colonize the wound at its very early stage and in addition to their protective immune role seem to organize the activity of other cell types at the following stages of the healing. Their benefit to this process is however controversial, as macrophages are described to promote the speed of healing but may also favour the fibrosis resulting from it in scars. Moreover wound healing defects are associated with abnormalities in the inflammatory phase. In this review, we summarise our knowled... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959834 Sun, 12 Sep 2010 08:54:03 +0100 3959834 http://www.medworm.com/index.php?rid=3959833&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830236%26dopt%3DAbstract In this study, we investigated the mechanism by which β-catenin is regulated in breast cancer cells. Our analysis of the gene promoter of β-catenin revealed multiple putative STAT3 binding sites. In support of the concept that STAT3 is a transcriptional regulator for β-catenin, results from our chromatin immunoprecipitation studies showed that STAT3 binds to two of the three potential STAT3-binding sites in the gene promoter of β-catenin (-856 and -938). Using our generated MCF-7 cell clones that carry an inducible STAT3C construct, we found that the expression levels of STAT3C significantly correlated with the transcriptional activity of β-catenin. Similar observations were made when we subjected two breast cancer cell lines (MCF-7 and BT-474) to STAT3 knock-down or transie... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959833 Sun, 12 Sep 2010 08:54:03 +0100 3959833 http://www.medworm.com/index.php?rid=3959832&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830237%26dopt%3DAbstract Authors: Lin WL, Wszolek ZK, Dickson DW Hereditary diffuse leukoencephalopathy with spheroids (HDLS) is a rare autosomal dominant disorder characterized by cerebral white matter degeneration with myelin loss and axonal swellings (spheroids) leading to progressive cognitive and motor dysfunction. Histopathology of HDLS has been well characterized, but ultrastructural details are lacking. Here we report ultrastructural and immunoelectron microscopic characterization of spheroids and capillary basal lamina in white matter of HDLS brains. Spheroids had thin or discontinuous or no myelin sheaths. They contained various combinations of aggregated neurofilaments (NF), cytoplasmic organelles, dense bodies, and laminated figures. Aggregated filaments labeled with antibodies to phosphorylated NF... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959832 Sun, 12 Sep 2010 08:54:03 +0100 3959832 http://www.medworm.com/index.php?rid=3959831&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830238%26dopt%3DAbstract In conclusion, our data indicate hepatocytes as cellular source of elevated hepatic as well as circulating RANTES levels in response to hepatic steatosis. Noteworthy, upregulation of RANTES in response to lipid accumulation occurs in the absence of relevant inflammation, which further indicates that hepatic steatosis per se has pathophysiological relevance and should not be considered as benign. PMID: 20830238 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959831 Sun, 12 Sep 2010 08:54:03 +0100 3959831 http://www.medworm.com/index.php?rid=3959830&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830239%26dopt%3DAbstract Authors: Bellayr IH, Gharaibeh B, Huard J, Li Y The liver is unique for its ability to regenerate after injury, however, critical injuries or disease cause it to lose this quality. Stem cells have been explored as a possibility to restore the function of seriously damaged livers, based on their self-renewability and multiple differentiation capacity. These experiments examine the ability of muscle derived stem cells (MDSCs) to differentiate into hepatocyte-like cells in vitro and acquire functional liver attributes for repairing damaged livers. In vitro experiments were performed using MDSCs from postnatal mice and mouse hepatocyte cell lines. Our data revealed that MDSCs differentiated into hepatocyte-like cells and expressed liver cell markers, albumin, hepatocyte nuclear factor 4ÃŽÂ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959830 Sun, 12 Sep 2010 08:54:03 +0100 3959830 http://www.medworm.com/index.php?rid=3959829&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830240%26dopt%3DAbstract CONCLUSION: MORPHOPROTEOMIC, HISTOPATHOLOGIC AND MORPHOMETRIC FINDINGS IN THIS STUDY OF DIFFUSE PHHI COINCIDE WITH EXISTING GENOMIC AND SIGNAL TRANSDUCTION DATA IN: 1) supporting a role for a constitutively activated and overexpressed mTORC1 pathway in the acinar pancreas in its pathogenesis; 2) reaffirming transdifferentiation of acinar-to-islet cells; 3) raising the possibility of rapamycin as a therapeutic option in PHHI. PMID: 20830240 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959829 Sun, 12 Sep 2010 08:54:03 +0100 3959829 http://www.medworm.com/index.php?rid=3959828&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830241%26dopt%3DAbstract Authors: Domingo DS, Camouse MM, Hsia AH, Matsui M, Maes D, Ward NL, Cooper KD, Baron ED Epigallocatechin-3-gallate (EGCG) is the main polyphenol component of green tea. This compound exhibits antioxidant, immunomodulatory, photoprotective, anti-angiogenic, and anti-inflammatory properties. We conducted a small randomized, double blind, split face trial using a cream containing 2.5% w/w of EGCG. Four healthy volunteers with significant erythema and telangiectasia on the face applied EGCG cream to one side of the face, and vehicle control cream to the other, twice daily for six weeks. After six weeks, biopsies were taken from EGCG and vehicle treated sites. Immunohistochemistry was used to measure VEGF and HIF-1α. HIF-1α expression was decreased in EGCG treated sites, such that 28... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959828 Sun, 12 Sep 2010 08:54:03 +0100 3959828 http://www.medworm.com/index.php?rid=3959827&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830242%26dopt%3DAbstract Authors: Ozeki T, Natori T Many anticancer drugs are developing until now. However, conventional anticancer drugs causes damage to not only cancer cells but also non-cancerous tissues and cells. Therefore, the development of new drugs are anticipated.HepG2 cell proliferation in cell culture was significantly inhibited by gabexate mesilate. In TUNEL method, a significant amount of HepG2 cells cultured with gabexate mesilate showed a decrease in the number of total cells and an increased in the number of positive cells. Further immunohistochemical staining for P-53,ss-DNA and caspase 3 showed samely a decrease in the number of total cells and an increase in the number of positive cells. The staining for bcl2 showed a decrease in the number of total cells and no remarkable change in the n... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959827 Sun, 12 Sep 2010 08:54:03 +0100 3959827 http://www.medworm.com/index.php?rid=3959826&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830243%26dopt%3DAbstract We describe a case of an 8-month-old Caucasian baby who presented with prominence of left eye and fever without lymphadenopathy or hepatosplenomegaly. Bone survey showed diffuse periosteal reaction in the femur, pelvis, maxillary and orbital bones (with fracture). CBC revealed normal white blood cell count with increased blasts, mild anemia and moderate thrombocytopenia. Bone marrow biopsy showed increased myeloblasts with bilineage dysplasia and 3-4+ reticulin fibrosis. Flow cytometry revealed blasts positive for CD34, CD33, and MPO and negative for CD7, CD13, and HLA-DR. Trisomy 10 was demonstrated by chromosome analysis and fluorescence in-situ hybridization. The patient received induction chemotherapy and achieved complete clinical and hematologic remission at day 28. However, he relap... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959826 Sun, 12 Sep 2010 08:54:03 +0100 3959826 http://www.medworm.com/index.php?rid=3959825&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830244%26dopt%3DAbstract We describe the clinical course and have characterised anatomically and genetically a unique case of a newborn with bilateral hypoplasia of pulmonary arteries, consecutive extremely hypoplastic lung tissue and associated unilateral renal agenesis. Intrauterine oxygenation by the placenta seemed to have allowed normotrophic body maturity but immediately after delivery, in the third trimester, progressive hypoxemia developed and the newborn succumbed to acute respiratory failure. Genetic analysis by array-based comparative genomic hybridisation and quantitative PCR revealed duplication of 1p21, which, however, might not be the disease causing aberration. This case might represent an extreme form of previously reported, rare cases with simultaneous dysorganogenesis of lungs and kidneys. P... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959825 Sun, 12 Sep 2010 08:54:03 +0100 3959825 http://www.medworm.com/index.php?rid=3959824&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830245%26dopt%3DAbstract CONCLUSION: Although primary seminoma of the lung is rare in old male population, the diagnosis should be taken into serious consideration in order to improve the treatment. And in this case, primary lung seminoma is associated with high degree of malignancy and metastasis. PMID: 20830245 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959824 Sun, 12 Sep 2010 08:54:03 +0100 3959824 http://www.medworm.com/index.php?rid=3959823&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830246%26dopt%3DAbstract Authors: Henderson-Jackson E, Nasir NA, Hakam A, Nasir A, Coppola D Primary lymphoepithelioma-like carcinomas (LELC) of the hepatobiliary tract are quite rare and the majority are associated with the Epstein-Barr virus (EBV). Here we report an unusual case of intrahepatic cholangiocarcinoma (ICC), admixed with LELC in a 63 year-old Filipino woman who presented clinically with right flank and back pain. Histologically, the tumor showed a dense lymphocytic infiltrate, predominantly composed of CD3 (+) T cells, and two components: an undifferentiated carcinoma, morphologically similar to nasopharyngeal carcinoma, and a poorly differentiated ICC intimately admixed. Immunohistochemical studies revealed that both components were immunoreac-tive for AE1/AE3, cytokeratin 7 and, focally, for mo... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959823 Sun, 12 Sep 2010 08:54:03 +0100 3959823 http://www.medworm.com/index.php?rid=3959822&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20830247%26dopt%3DAbstract Monoclonal antibody Cam 5.2 targeted mainly CK8, but not CK18 - Comment on: "Chromophobe renal cell carcinoma with liposarcomatous dedifferentiation - report of a unique case. Int J Clin Exp Pathol. 2010 May 5; 3 (5):534-40.". Int J Clin Exp Pathol. 2010;3(7):742 Authors: Leei CK, Lin WL, Han CP PMID: 20830247 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3959822 Sun, 12 Sep 2010 08:54:03 +0100 3959822 http://www.medworm.com/index.php?rid=3799549&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661403%26dopt%3DAbstract Authors: Pansuriya TC, Kroon HM, Bovée JV Enchondromatosis is a rare, heterogeneous skeletal disorder in which patients have multiple enchondromas. Enchondromas are benign hyaline cartilage forming tumors in the medulla of metaphyseal bone. The disorder manifests itself early in childhood without any significant gender bias. Enchondromatosis encompasses several different subtypes of which Ollier disease and Maffucci syndrome are most common, while the other subtypes (metachondromatosis, genochondromatosis, spondyloenchondrodysplasia, dysspondyloenchondromatosis and cheirospondyloenchondromatosis) are extremely rare. Most subtypes are non-hereditary, while some are autosomal dominant or recessive. The gene(s) causing the different enchondromatosis syndromes are largely unknown. They ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799549 Thu, 29 Jul 2010 20:30:03 +0100 3799549 http://www.medworm.com/index.php?rid=3799548&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661404%26dopt%3DAbstract Authors: Santos RX, Correia SC, Wang X, Perry G, Smith MA, Moreira PI, Zhu X Alzheimer's disease is a progressive neurodegenerative disorder, either assuming a sporadic, age-associated, late-onset form, or a familial form, with early onset, in a smaller fraction of the cases. Whereas in the familial cases several mutations have been identified in genes encoding proteins related with the pathogenesis of the disease, for the sporadic form several causes have been proposed and are currently under debate. Mitochondrial dysfunction has surfaced as one of the most discussed hypotheses acting as a trigger for the pathogenesis of Alzheimer's disease. Mitochondria assume central functions in the cell, including ATP production, calcium homeostasis, reactive oxygen species generation, and apoptot... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799548 Thu, 29 Jul 2010 20:30:03 +0100 3799548 http://www.medworm.com/index.php?rid=3799547&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661405%26dopt%3DAbstract Authors: Karp JK, Akpek EK, Anders RA Based on the revised criteria of the American-European Consensus Group, we retrospectively established the diagnosis of primary or secondary Sjögren's syndrome for 202 patients referred to a Sjögren's syndrome clinic. Of these, 58 patients and 8 patients fulfilled criteria for primary and secondary Sjögren's syndrome, respectively. Of the 58 patients with primary Sjögren's syndrome, one (1.7%) had definite autoimmune hepatitis, as defined by the International Autoimmune Hepatitis Group diagnostic criteria. One additional symptomatic patient who did not fulfill criteria for primary Sjögren's syndrome had definite autoimmune hepatitis. None of the patients with secondary Sjögren's syndrome had definite autoimmune hepatitis. Two (1%)... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799547 Thu, 29 Jul 2010 20:30:03 +0100 3799547 http://www.medworm.com/index.php?rid=3799546&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661406%26dopt%3DAbstract Authors: Inoue M, Matsuyama M, Shiono H, Honda O, Sumikawa H, Tomiyama N, Okumura M BUF/Mna rats develop thymomas spontaneously, which histologically mimic human thymomas. Although neoplasms in this rat strain contain a large number of immature lymphocytes, the phenotype has not been sufficiently assessed. We characterized T cell phenotypes in tumors from BUF/Mna rats in the present study. We also analyzed BUF/Mna-Rnu/+ rats, a heterozygous strain with suppressive thymomagenesis, and compared the histology and T cell maturation with those from the BUF/Mna rats. A total of 11 BUF/Mna and 10 BUF/Mna-Rnu/+ rats were used. Three-color flow cytometry was performed with anti-CD3, CD4, and CD8 antibodies to identify infiltrated lymphocytes, while tumor histology was evaluated with hematoxylin... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799546 Thu, 29 Jul 2010 20:30:03 +0100 3799546 http://www.medworm.com/index.php?rid=3799545&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661407%26dopt%3DAbstract In this study, we correlated the clinical, histologic and array CGH findings of 10 spiztoid melanocytic neoplasms. Our study shows that the clinical and histologic changes correlate well with the molecular findings by array CGH. Further that array CGH can be used to help classify and predict behavior of spitzoid melanocytic neoplasms. A limited variety of copy number aberrations including gains of 11p and much more rarely 7q may be seen in spitz nevi. Additionally in this report we present the first case of a typical spitz nevus with copy number gains involving both 7q and 11p. Conversely, melanomas with spitzoid features typically have multiple chromsomal copy number aberrations involving a variety of loci. A smaller number of chromosomal aberrations, possibly a single aberrant locus, may... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799545 Thu, 29 Jul 2010 20:30:03 +0100 3799545 http://www.medworm.com/index.php?rid=3799544&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661408%26dopt%3DAbstract Authors: Bohn OL, Fuertes-Camilo M, Navarro L, Saldivar J, Sanchez-Sosa S BLBC represents a distinctive group of invasive breast carcinomas with specific genotype and immunopro-file. BLBC is usually defined by gene expression profiling and is currently associated with poor outcome. BLBCs are estrogen receptor (ER) negative, progesterone receptor (PgR) negative, HER2 negative, and usually show a variable expression of basal cytokeratins (CKs), EGFR and CD117. p16 (INK4a) is a tumor suppressor protein, encoded by the CDKN2A gene, which regulates cell cycle. The reported association of abnormalities in the p16/Rb pathway with increased risk of malignancy prompted us to determine the expression of p16(INK4a) in a group of BLBC; the results were compared with a group of high-grade invasive ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799544 Thu, 29 Jul 2010 20:30:03 +0100 3799544 http://www.medworm.com/index.php?rid=3799543&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661409%26dopt%3DAbstract Authors: Zakharov V, Lin HK, Azzarello J, McMeekin S, Moore KN, Penning TM, Fung KM The diagnosis of endometrial hyperplasia and endometrial type adenocarcinoma arising within the uterine cavity has long been rested on morphologic criteria. Although distinction between normal endometrial epithelium from adenocarcinoma is usually straightforward, the separation between normal and hyperplastic endometrium, particularly those cases without atypia, can be a diagnostic challenge. The same is true in separation of hyperplastic endometrium with atypia from endometrial-type endometrial adenocarcinoma. Type 2 3alpha-/type 5 17beta-hydroxysteroid dehydrogenase (HSD) (AKR1C3) is a multifunctional enzyme involved in androgen, estrogen, progesterone, and pros-taglandin metabolism. Its expression ha... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799543 Thu, 29 Jul 2010 20:30:03 +0100 3799543 http://www.medworm.com/index.php?rid=3799542&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661410%26dopt%3DAbstract Authors: Luo X, Yan R Since BACE1 was reported as the beta-secretase in Alzheimer's disease (AD) over ten years ago, encouraging progress has been made toward understanding the cellular functions of BACE1. Genetic studies have further confirmed that BACE1 is essential for processing amyloid precursor protein (APP) at the beta-secretase site. Only after this cleavage can the membrane-bound APP C-terminal fragment be subsequently cleaved by gamma-secretase to release so-called AD-causing Abeta peptides. Hence, in the past decade, a wide variety of BACE1 inhibitors have been developed for AD therapy. This review will summarize the major historical events during the evolution of BACE1 inhibitors designed through different strategies of drug discovery. Although BACE1 inhibitors are expected... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799542 Thu, 29 Jul 2010 20:30:03 +0100 3799542 http://www.medworm.com/index.php?rid=3799541&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661411%26dopt%3DAbstract We report a case of primary small cell carcinoma of the breast with coexisting carcinoma in situ in which the invasive carcinoma and in situ component both expressed neuroendocrine markers and Thyroid transcription factor-1 (TTF-1) by immunohistochemistry. Expression of neuroendocrine markers and TTF-1 in the in situ component allowed a highly confident diagnosis of primary small cell carcinoma of the breast. To our knowledge there is only one previous report of TTF-1 expressing in situ carcinoma associated with a primary SmCC of the breast. PMID: 20661411 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799541 Thu, 29 Jul 2010 20:30:03 +0100 3799541 http://www.medworm.com/index.php?rid=3799540&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661412%26dopt%3DAbstract Authors: Takeuchi T, Iwasaki S, Miyazaki J, Nozaki Y, Takahashi M, Ono M, Saibara T, Furihata M Angiosarcoma involving the serous membrane may mimic mesothelioma; therefore, the term "pseudomesotheliomatous angiosarcoma" has been suggested for this entity. However, the pathogenesis of pseudomesotheliomatous angiosarcoma remains unclear. Here, we report an autopsy case of splenic angiosarcoma, which systemically metastasized to the serous membrane of both the peritoneum and pleura, closely resembling a mesothelioma. The spindle-shaped tumor cells exhibited marked invasion of the lymphatic vessels and invaded the serous membrane causing thickening of the fibrous tissues like desmoplastic mesothelioma. In the present case, immunohistochemical staining showed that the tumor expressed not o... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799540 Thu, 29 Jul 2010 20:30:03 +0100 3799540 http://www.medworm.com/index.php?rid=3799539&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20661413%26dopt%3DAbstract Authors: Dickson DW, Li S, Iacobuzio-Donahue C, Wang D PMID: 20661413 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3799539 Thu, 29 Jul 2010 20:30:03 +0100 3799539 http://www.medworm.com/index.php?rid=3738738&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606727%26dopt%3DAbstract Authors: Agaimy A Following the successful introduction of the receptor tyrosine kinase inhibitors (TKI) as the mainstay for the treatment of advanced and metastatic gastrointestinal stromal tumor (GIST), GIST has received a special attention in the recent literature. This resulted in major achievements on the surgical pathology diagnosis and improved our understanding of the molecular biology of the disease. Availability of the effective TKI therapy has emphasized the need for a more reliable and reproducible system for assessment of the malignant potential in GIST to allow for an optimal individualized patient treatment. All of the risk stratification systems proposed so far have emphasized the value of tumor size, mitotic count and anatomic site for risk estimation, at the same time... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738738 Sat, 10 Jul 2010 05:42:03 +0100 3738738 http://www.medworm.com/index.php?rid=3738737&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606728%26dopt%3DAbstract In this study, we used an animal model of NASH to examine hepatic AdipoR2. Obese fa/fa Zucker rats fed a high-fat and high-cholesterol (HFC) diet spontaneously developed fatty liver with inflammation and fibrosis, characteristic of NASH, after 4, 8, or 12 weeks of HFC diet consumption. AdipoR2 expression was significantly decreased, whereas the expression of genes related to NADPH oxidase complex were increased. As a result of the decrease in AdipoR2 expression, the mRNA expression of genes located downstream of AdipoR2, i.e., Cu-Zn superoxide dismutase (Cu-Zn SOD) and Mn-SOD, also decreased. Furthermore, the expression of genes related to inflammation was increased. Increased oxidative stress and inflammation by down-regulation of AdipoR2 may contribute to the progression of NASH. Thus, t... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738737 Sat, 10 Jul 2010 05:42:03 +0100 3738737 http://www.medworm.com/index.php?rid=3738736&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606729%26dopt%3DAbstract Authors: Yin DT, Wang L, Sun J, Yin F, Yan Q, Shen R, He G, Gao JX The role of aberrant methylation of fragile histidine triad (FHIT) promoters in the differentiated thyroid carcinoma (DTC) is not yet clear. Therefore, we investigated the association of the status of FHIT promoter methylation and FHIT protein expression with the clinicopathological progression of DTC, using PCR-based methylation assay and immunohistochemical technique. While no FHIT gene promoter methylation was observed in the matched non-cancerous epithelium (NCE) specimens, 24.6% of DTC samples demonstrated methylation in the FHIT promoter region. The protein expression of FHIT in NCE and DTC was 100.0% and 41.5% (P<0.01), respectively. There was a negative correlation between promoter methylation and protein exp... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738736 Sat, 10 Jul 2010 05:42:03 +0100 3738736 http://www.medworm.com/index.php?rid=3738735&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606730%26dopt%3DAbstract Authors: Zhang L, Smyrk TC Autoimmune pancreatitis (AIP) is a rare form of chronic pancreatitis that is characterized by lymphoplasmacytic infiltrate, storiform fibrosis, obliterative phlebitis, and increased IgG4(+) plasma cells. Serum IgG4 levels usually are elevated. Patients with AIP frequently have disease affecting other organs or sites; these tissues show similar histologic changes, including increased IgG4(+) plasma cell infiltrate and response to corticosteroid therapy. A new clinicopathologic concept of IgG4-related systemic disease (ISD) has been proposed. These diseases often are not limited to the pancreas, and the pancreas may not be involved at all. In this article, we review the literature and our own experience to detail the clinicopathologic features of AIP and extrap... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738735 Sat, 10 Jul 2010 05:42:03 +0100 3738735 http://www.medworm.com/index.php?rid=3738734&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606731%26dopt%3DAbstract In conclusion, the transcriptional induction of FASN expression in hepatic steatosis is impaired in NASH, while hepatic inflammation in the absence of steatosis does not affect FASN expression, suggesting that FASN may serve as a new diagnostic marker or therapeutic target for the progression of NAFLD. PMID: 20606731 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738734 Sat, 10 Jul 2010 05:42:03 +0100 3738734 http://www.medworm.com/index.php?rid=3738733&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606732%26dopt%3DAbstract This study was aimed at determining whether CD44 and p53 may serve as indicators of malignant progression of neurofibroma. CD44, a transmembrane glycoprotein receptor for hyaluronic acid, and participates in cell-extracellular matrix interactions and migration. CD44 may play a vital role, either through under or overexpression, with invasion and metastases of tumors, altering their ability to infiltrate the adjacent tissue. The tumor suppressor gene, p53, has also been implicated in malignant progression of various human tumors including malignant peripheral nerve sheath tumors (MPNST). A total of 44 tumors from 33 patients with NF1 were evaluated with an anti-human CD44H, CD44 splice variant v6 and anti-p53 monoclonal antibodies. Morphologic expression patterns of expression were evaluate... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738733 Sat, 10 Jul 2010 05:42:03 +0100 3738733 http://www.medworm.com/index.php?rid=3738732&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606733%26dopt%3DAbstract Authors: Shackelford RE, Bui MM, Coppola D, Hakam A Nicotinamide phosphoribosyltransferase (Nampt) catalyzes the rate-limiting step of nicotinamide adenine dinucleotide (NAD(+)) synthesis and is required for cell growth, survival, DNA replication and repair, and angiogenesis. Nampt expression increases gene expression which promotes cell survival and increases SirT1 activity, promoting angiogenesis, and it is increased in several human malignancies. Recently, others have shown that ovarian serous adenocarcinomas (OSAs) express high levels of activated Stat3. Since Nampt expression is increased by Stat3, we hypothesized that Nampt protein might be highly expressed in OSAs. Using tissue microarray (TMA) and the avidin-biotin complex immunohistochemical technique we examined Nampt express... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738732 Sat, 10 Jul 2010 05:42:03 +0100 3738732 http://www.medworm.com/index.php?rid=3738731&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606734%26dopt%3DAbstract Authors: Zhang G, Lu Q, Yin H, Wen H, Su Y, Li D, Xiao R Retiform hemangioendothelioma (RH) is an extremely rare low-grade angiosarcoma mainly involving the skin and subcutaneous tissue. Clinically patients often present with an asymptomatic slow-growing solitary nodular or plaque-like lesion. RH is characterized by frequent local recurrences but a very low metastatic rate. Here we reported a case of RH in a 61-year-old Chinese woman who presented with a rapid growing cutaneous plaque-like lesion on her right scalp, followed by another lesion behind the right ear. The lesions were associated with paroxysmal sharp needle-stabbing like headache. She underwent wide excision and skin engraftment. Three months post surgery, she experienced tumor recurrence, and died 9 months after the initi... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738731 Sat, 10 Jul 2010 05:42:03 +0100 3738731 http://www.medworm.com/index.php?rid=3738730&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606735%26dopt%3DAbstract Authors: Petersson F, Michal M, Franco M, Hes O Sarcomatous transformation of chromophobe renal cell carcinoma (CRCC) is a well recognized phenomenon. Of the published cases with sarcomatous transformation of CRCC, none have shown liposarcomatous differentiation. Out of a cohort of 250 cases of CRCC, 19 (7.6%) showed sarcomatous differentiation. In one case (female, age 46 years), the sarcomatous component of the tumor displayed histological features of a pleomorphic liposar-coma. Light microscopic examination revealed a biphasic pattern with a chromophobe renal cell carcinoma(CRCC) and a high-grade sarcomatous component containing large pleomorphic lipoblasts. In several areas both components were intermingled. The conventional CRCC component showed classic histological features with ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738730 Sat, 10 Jul 2010 05:42:03 +0100 3738730 http://www.medworm.com/index.php?rid=3738729&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606736%26dopt%3DAbstract Authors: Terada T Cutaneous angiosarcoma is a very rare but aggressive tumor. Angiosarcoma of the scalp is very rare, and a review of the world literature revealed less than 60 cases. Here, the author reports a case of poorly differentiated angiosarcoma of the scalp. The pathological diagnosis was very difficult. A 70-year-old Japanase man was admitted to our hospital complaining of red mass of the scalp. An excisional biopsy was done. The biopsy showed proliferation of malignant spindle cells in the dermis. Apparent differentiation was not recognized. Invasion into the lateral dermis and subcutis was recognized. There were many mitotic figures and a few foci of necrosis. The size was 2 x 2 x 3 cm. Intracytoplasmic vacuoles were recognized in the malignant tumor cells in some places. A... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738729 Sat, 10 Jul 2010 05:42:03 +0100 3738729 http://www.medworm.com/index.php?rid=3738728&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606737%26dopt%3DAbstract Authors: Pan L, Peng L, Jean-Gilles J, Zhang X, Wieczorek R, Jain S, Levine V, Osman I, Prieto VG, Lee P Carney complex is a syndrome that may include cardiac and mucocutaneous myxomas, spotting skin pigmentation, and endocrine lesions. Many patients with Carney complex have been shown to have a stop codon mutation in the PRKAR1A gene in the 17q22-24 region. Here we present the case of a 57 year-old man with multiple skin lesions and cardiac myxomas. Histology of the skin lesions showed lentigenous melanocytic hyperplasia and cutaneous myxomas, confirming the diagnosis of Carney complex. Lesional and control normal tissue from the patient were identified and sequenced for the PRKAR1A gene. A germline missense mutation was identified at exon 1A. This is the first report of this mutation... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738728 Sat, 10 Jul 2010 05:42:03 +0100 3738728 http://www.medworm.com/index.php?rid=3738727&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20606738%26dopt%3DAbstract We describe herein two such cases. Case 1 was a 59-yr-old woman who presented with signs of ileus and a large mass submitted as Meckel diverticulum. The resection specimen showed a large GIST with diverticulum-like and solid areas. The diverticular component showed a diffuse proliferation of spindle cells extending for several centimetres from the solid tumor replacing the full thickness of the gut wall and lined by intact mucosa. Mutation analysis revealed a combined deletion/insertion in c-KIT exon 11 (V560delEins) in both the solid and the diffuse tumor component. Case 2 was a 66-yr-old man who underwent segmental sigmoid colon resection for adenocarcinoma in a villous adenoma. Random sections from grossly unremarkable colonic wall showed a diffuse proliferation of CD117+/CD34+ spindle ... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3738727 Sat, 10 Jul 2010 05:42:03 +0100 3738727 http://www.medworm.com/index.php?rid=3587652&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490325%26dopt%3DAbstract In conclusion, piwil2 is expressed in various stages of breast cancers and has the potential to be used a novel biomarker. PMID: 20490325 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587652 Sat, 22 May 2010 13:45:03 +0100 3587652 http://www.medworm.com/index.php?rid=3587651&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490326%26dopt%3DAbstract Authors: Jedlicka P Since its first description by James Ewing in 1921, Ewing Sarcoma has been a cryptic malignancy. A poorly differentiated tumor of uncertain histogenesis and aggressive biologic behavior, it is the second most common malignancy of bone and soft tissue affecting adolescents and young adults. Some two decades ago, the understanding of Ewing Sarcoma biology took a leap forward with the identification of recurrent EWS/Ets fusions, which drive onco-genesis in this disease. A further leap forward occurred over the last half decade with the application of gene silencing, global expression profiling and primary cell culture technologies to the study of this disease. Resulting work has revealed EWS/Ets fusions to be surprisingly versatile regulators of gene expression, and ha... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587651 Sat, 22 May 2010 13:45:03 +0100 3587651 http://www.medworm.com/index.php?rid=3587650&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490327%26dopt%3DAbstract Authors: Okudela K, Woo T, Mitsui H, Yazawa T, Shimoyamada H, Tajiri M, Ogawa N, Masuda M, Kitamura H We have established a concise sub-typing system suitable for predicting the postoperative outcome in cases of stage I lung adenocarcinoma (ADC), using morphometric profiling. The association between postoperative disease recurrence and a variety of morphological features including histological architecture, cell type, cytoplasmic color/internal structure, nuclear shape/size, chromatin pattern, and nucleoli count/remarkableness, was analyzed. Histological architecture had the most prognostic value and could be subdivided into low-grade (bronchioloalveolar, papillary and tubular: "tubular" in this paper is defined as a tubular or glandular structure lined with single-layered neoplastic c... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587650 Sat, 22 May 2010 13:45:03 +0100 3587650 http://www.medworm.com/index.php?rid=3587649&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490328%26dopt%3DAbstract This article focuses on the main classification of primary lung carcinoma - small cell carcinoma, adenocarcinoma and squamous cell carcinoma - with potential mimics discussed under each tumor category. We have attempted to separate pitfalls from common potential mimics and have suggested general rules when such pitfalls are encountered. PMID: 20490328 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology) International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587649 Sat, 22 May 2010 13:45:03 +0100 3587649 http://www.medworm.com/index.php?rid=3587648&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490329%26dopt%3DAbstract Authors: Wuebbles RD, Long SW, Hanel ML, Jones PL The genetic lesion leading to facioscapulohumeral muscular dystrophy (FSHD) is a dominant deletion at the 4q35 locus. The generally accepted disease model involves an epigenetic dysregulation in the region resulting in the upregulation of one or more proximal genes whose overexpression specifically affects skeletal muscle. However, multiple FSHD candidate genes have been proposed without clear consensus. Using Xenopus laevis as a model for vertebrate development our lab has studied the effects of overexpression of the FSHD candidate gene ortholog, frg1 (FSHD region gene 1), showing that increased levels of frg1 systemically led specifically to an abnormal musculature and increased angiogenesis, the two most prominent clinical features o... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587648 Sat, 22 May 2010 13:45:03 +0100 3587648 http://www.medworm.com/index.php?rid=3587647&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490330%26dopt%3DAbstract In this study, we evaluated the ability of current biopsy protocols to predict cancer characteristics in radical prostatectomy (RP) specimens. We reviewed 4437 cases from 2000 to 2008 in our PowerPath database, and identified 158 patients with low-risk cancer, defined as a pre-biopsy PSA level </= 10 ng/mL, unilateral, low tumor volume (</=5%) and low to intermediate Gleason score (GS</=6) on first positive prostate biopsy. The pathological characteristics of subsequent RP specimens were reviewed. We found that, of 158 patients with these criteria, 117 (74%) had bilateral cancer, 49 (31%) had increased tumor volume (>/= 10%), and 46 (29%) were upgraded to GS >/= 7 at RPs. When patients were stratified by total biopsy core numbers, extended biopsy core protocols were not sign... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587647 Sat, 22 May 2010 13:45:03 +0100 3587647 http://www.medworm.com/index.php?rid=3587646&cid=s_38149_32_f&fid=38149&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20490331%26dopt%3DAbstract In this study, we will examine the efficacy of sorafenib on a human neuroblastoma cell line (SK-N-AS) and also investigate its possible mechanisms. After cells reached 50-60% confluence, they were treated with various concentrations of sorafenib (0, 0.1, 1, 5, 10 and 20 muM) for different periods of time. The cell viability and apoptosis were determined by MTS colorimetric assay and TUNEL, respectively. Phosphorylation of Akt1/2/3 (p-Akt1/2/3), extracellular signal-regulated kinase 1/2 (p-ERK1/2), STAT3 (p-STAT3), and AMP-activated protein kinase alpha subunit (p-AMPKalpha) were determined with Western blot. The results indicate that as early as 2 hours post-treatment, cell viability was significantly decreased at 10 muM concentration. In 24 hours or longer treatment groups, sorafenib at 5... International Journal of Clinical and Experimental Pathology journals http://www.medworm.com/rss/comments.php?id=3587646 Sat, 22 May 2010 13:45:03 +0100 3587646