International Journal of Experimental Pathology via MedWorm.com

Glucose transporter protein 1 expression in mucoepidermoid carcinoma of salivary gland: correlation with grade of malignancy

International Journal of Experimental Pathology — Sat, 23 Jan 2010 00:00:00 +0100

Mucoepidermoid carcinoma (MEC), the most common primary salivary malignancy, shows great variability in clinical behaviour, thus demanding investigation to identify of prognostic markers. Since Warburg's studies, unrestricted cell growth during tumorigenesis has been linked to altered metabolism, implying hypoxic stimulation of glycolysis and diminished contribution of mitochondrial oxidative phosphorylation to cellular ATP supply. Hypothesizing that the study of MEC metabolic status could lead to the discovery of prognostic markers, we investigated by immunohistochemistry the expression of glucose transporter 1 (Glut-1), mitochondrial antigen and peroxiredoxin I (Prx I) in samples of MEC from different histological grades. Our results showed that mitochondrial antigen and Prx I were expre...

The Mongolian gerbil as a model for inflammatory bowel disease

International Journal of Experimental Pathology — Fri, 22 Jan 2010 00:00:00 +0100

Mongolian gerbils are used as biomedical research models for a variety of diseases and are in some cases suited better than other rodents for basic research and therapeutic studies. The aim of this study was to establish and characterize a dextran sulphate sodium (DSS)-induced model in gerbils for the human inflammatory bowel disease (IBD) and to utilize them for a therapeutic study in vivo. Four concentrations (0.5%, 1%, 2% and 4%) of DSS were administered via drinking water for 7 days; based on these results, a concentration of 3% DSS was given for 9 days in a second approach. Fluid uptake and general clinical condition were assessed daily using a clinical score. Caecum and colon were scored histologically. Fluid uptake was affected by addition of DSS to the drinking water. First clinica...

Disorders related with ageing in the gerbil female prostate (Skene's paraurethral glands)

International Journal of Experimental Pathology — Tue, 22 Dec 2009 00:00:00 +0100

The female organs, which are regulated by steroid hormones, are targets of studies especially those related to senescence. However, although the female prostate is an organ influenced by hormones and susceptible to lesions, there is still little information about its histopathology. Thus, given the morphophysiological similarity between the prostate in women and female gerbils, the present study aimed to identify the spontaneous histopathological changes in this rodent to provide contributions to the understanding of lesions that also affect the human female prostate. The structural, ultrastructural, immunohistochemical, morphometric-stereological and serological aspects, as well as the quantification of the incidence, multiplicity and percentage of acini affected by different lesions were...

A quantitative study of lung dysfunction following haemorrhagic shock in rats

International Journal of Experimental Pathology — Tue, 22 Dec 2009 00:00:00 +0100

Haemorrhagic shock occasionally causes an episode of lung dysfunction, the severity of which appears to correlate with fatal outcome. Our previous study indicated that proinflammatory cytokines, such as tumour necrosis factor (TNF)-[alpha] and interleukin (IL)-1[beta], played a key role in the development of lung dysfunction through recruitment of activated neutrophils by causing pulmonary endothelial cell damage. Here, we examined this issue quantitatively by grading four groups of severity of bleeding in rats. As the amount of bleeding increased, the expression of mRNA for TNF-[alpha] and IL-1[beta] in the lung tissue and the pulmonary serum levels of both cytokines increased progressively up to 5 h, and the frequency of activated neutrophils increased likewise. The lung dysfunction indi...

Proliferation and apoptotic rates and increased frequency of p63-positive cells in the prostate acinar epithelium of alloxan-induced diabetic rats

International Journal of Experimental Pathology — Tue, 22 Dec 2009 00:00:00 +0100

The effects of experimental type 1 diabetes were investigated in the acinar epithelium of rat ventral prostate, focusing on the rates of cell proliferation and the frequency of apoptosis and p63-positive cells. Type 1 diabetes was induced in adult male Wistar rats by a single alloxan administration (42 mg/kg b.w.) and its effects were analysed for 1 week and 3 months after the establishment of the disease. A group of diabetic rats was treated daily with 5 IU of insulin during 1 week after diabetes had been diagnosed. Immunocytochemical methods for the localization of cell proliferation antigen (PCNA), androgen receptor (AR) and p63 protein were carried out, and apoptotic cells were identified by TUNEL essay. In diabetic rats, testosterone levels reduced drastically after 1 week and in a lo...

Potential role of CXCL10 in the induction of cell injury and mitochondrial dysfunction

International Journal of Experimental Pathology — Tue, 22 Dec 2009 00:00:00 +0100

Chemokines have been known to play a critical role in pathogenesis of chronic pancreatitis and acinar cell death. However, the role played by one of the CXC chemokines: CXCL10 in regulation of acinar cell death has remained unexplored. Hence, this study was designed to assess the role of CXCL10 promoting apoptosis in ex vivo cultured acinar cells. Primary human pancreatic acinar cell cultures were established and exposed to varying doses of CXCL10 for different time intervals. Apoptotic induction was evaluated by both qualitative as well as quantitative analyses. Various mediators of apoptosis were also studied by Western blotting, membrane potential ([Psi]m) and ATP depletion in acinar cells. Analysis of apoptosis via DNA ladder and cell death detection [ndash] ELISA demonstrated that CXC...

Periadventitial delivery of anti-EGF receptor antibody inhibits neointimal macrophage accumulation after angioplasty in a hypercholesterolaemic rabbit

International Journal of Experimental Pathology — Sat, 12 Dec 2009 00:00:00 +0100

Monocyte recruitment and their differentiation into macrophages are both early events in native and accelerated atherosclerosis that follows angioplasty. We have investigated the putative functional role of the epidermal growth factor receptor (EGFR) present on rabbit monocytes/macrophages. The impact of periadventitial delivery of an EGFR-specific, blocking monoclonal antibody (ICR62, which inhibits EGF-binding to its receptor) was investigated in a rabbit model of accelerated atherosclerosis induced by a combination of carotid injury and 4 weeks of a 2% cholesterol-diet. Two weeks after the initiation of the diet, a balloon-catheter angioplasty of the left common carotid artery was performed and a collar placed around the injured carotid artery immediately, for the delivery of ICR62 anti...

Testis response to low doses of cadmium in Wistar rats

International Journal of Experimental Pathology — Thu, 10 Dec 2009 00:00:00 +0100

Although it is well known that cadmium (Cd) causes adverse effects on male rat reproductive organs, few studies have quantified alterations caused by its low doses. Quantification of these alterations, especially in the testis, was measured using morphometry. A single dose of cadmium chloride (1 or 1.2 mg/kg BW) was injected i.p. in adult rats, killed after 7 or 56 days. The lower dose caused slight alterations as measured by morphometrical analysis. The higher dose caused significant reduction in testis and epididymis weight, gonadossomatic index and length of seminiferous tubule (ST) after 7 and 56 days. Cadmium significantly reduced the ST diameter after 56 days. Decreased volume density of ST, after 7 and 56 days, was accompanied by an increase in interstitium volume density. The damag...

Increased calcium influx in the presence of ethanol in mouse pancreatic acinar cells

International Journal of Experimental Pathology — Sat, 05 Dec 2009 00:00:00 +0100

The effects of alcohol on Ca2+ signalling remains poorly understood. Here we have investigated the effects of acute ethanol exposure on Ca2+ influx in mouse pancreatic acinar cells. Cells were loaded with fura-2 and the changes in fluorescence were monitored by spectrofluorimetry and imaging analysis. Stimulation of cells with 20 pM cholecystokinin evoked an oscillatory pattern in [Ca2+]c, both in the presence and in the absence of extracellular Ca2+. Stimulation of cells with cholecystokinin in the presence of 50 mM ethanol led to a transformation of physiological oscillations into a single transient increase in [Ca2+]c. This effect was observed when Ca2+ was present in the extracellular medium, and did not appear in its absence. Addition of 1 mM CaCl2 to the extracellular medium, followi...

A comparative study of the spatial distribution of mast cells and microvessels in the foetal, adult human thymus and thymoma

International Journal of Experimental Pathology — Fri, 04 Dec 2009 00:00:00 +0100

Mast cells (MCs) are widely distributed in human and animal tissues and have been shown to play an important role in angiogenesis in normal and pathological conditions. Few data are available about the relationship between MCs and blood vessels in the normal human thymus, and there are virtually no data about their distribution and significance in thymoma. The aim of this study was to analyse the spatial distribution of MCs and microvessels in the normal foetal and adult thymus and thymoma. Twenty biopsy specimens of human thymus, including foetal and adult normal thymus and thymoma were analysed. Double staining with CD34 and mast cell tryptase was used to count both mast cells and microvessels in the same fields. Computer-assisted image analysis was performed to characterize the spatial ...

Myostatin and follistatin expression in skeletal muscles of rats with chronic heart failure

International Journal of Experimental Pathology — Fri, 04 Dec 2009 00:00:00 +0100

In conclusion, our data show that during chronic heart failure, decreased skeletal muscle trophism is combined with unchanged myostatin and reduced follistatin expression. (Source: International Journal of Experimental Pathology)

Calcium-binding proteins in skeletal muscles of the mdx mice: potential role in the pathogenesis of Duchenne muscular dystrophy

International Journal of Experimental Pathology — Thu, 03 Dec 2009 00:00:00 +0100

Duchenne muscular dystrophy is one of the most common hereditary diseases. Abnormal ion handling renders dystrophic muscle fibers more susceptible to necrosis and a rise in intracellular calcium is an important initiating event in dystrophic muscle pathogenesis. In the mdx mice, muscles are affected with different intensities and some muscles are spared. We investigated the levels of the calcium-binding proteins calsequestrin and calmodulin in the non-spared axial (sternomastoid and diaphragm), limb (tibialis anterior and soleus), cardiac and in the spared extraocular muscles (EOM) of control and mdx mice. Immunoblotting analysis showed a significant increase of the proteins in the spared mdx EOM and a significant decrease in the most affected diaphragm. Both proteins were comparable to th...

Perforin-expressing cytotoxic cells contribute to chronic cardiomyopathy in Trypanosoma cruzi infection

International Journal of Experimental Pathology — Wed, 28 Oct 2009 00:00:00 +0100

Understanding the dual participation of the immune response in controlling the invader and at the same time causing tissue damage might contribute to the design of effective new vaccines and therapies for Chagas disease. Perforin, a cytolytic protein product of killer cells, is involved in resistance to acute Trypanosoma cruzi infection. However, the contribution of perforin in parasite control and chronic chagasic cardiomyopathy is unclear. Perforin-positive cells were detected in the heart tissue during the acute and chronic phases of infection of C57BL/6 mice inoculated with low dose (102 parasites) of the Colombian T. cruzi strain. This protocol led to acute phase survival in both wild-type and perforin null (pfp[minus]/[minus]) mice lineages. During the chronic infection, parasitism a...

PIM-1 kinase expression in adipocytic neoplasms: diagnostic and biological implications

International Journal of Experimental Pathology — Wed, 28 Oct 2009 00:00:00 +0100

The differential diagnosis of soft tissue tumours poses a considerable challenge for pathologists, especially adipocytic tumours, as these may show considerable overlap in clinical presentation and morphological features with many other mesenchymal neoplasms. Hence, a specific and reliable marker that identifies adipocytic differentiation is much sought. We investigated the immunohistochemical expression of PIM-1 kinase in 35 samples of soft tissue tumours using tissue microarray technology and 49 full sections of adipocytic (n = 26) and non-adipocytic tumours (n = 23). Benign and malignant adipocytic tumours showed strong expression of PIM-1 while the non-adipocytic tumours were either negative or showed only weak staining for the protein. In myxoid liposarcomas, PIM-1 showed a distinct, ...

Lipid compounds of human Wharton's jelly and their alterations in preeclampsia

International Journal of Experimental Pathology — Wed, 28 Oct 2009 00:00:00 +0100

Wharton's jelly is a myxomatous substance which surrounds the umbilical cord vessels protecting them against extension, bending, twisting and compression. Very low number of cells in this tissue produce high amounts of extracellular matrix; collagen, hyaluronate and proteoglycans which bind large quantities of peptide growth factors (PGFs). Preeclampsia (the most common pregnancy-associated syndrome) is accompanied by a significant reduction in hyaluronate and a concomitant increase in sulphated glycosaminoglycans/proteoglycans content in Wharton's jelly. Such a phenomenon corresponds to an 'early ageing' of this tissue. We have evaluated the lipid composition of Wharton's jelly and its alteration in preeclampsia. Thin layer chromatography and high-performance liquid chromatography were em...

Reactive oxygen species promote angiogenesis in the infarcted rat heart

International Journal of Experimental Pathology — Tue, 15 Sep 2009 23:00:00 +0100

We examined the spatial and temporal relationship between oxidative stress and angiogenesis as well as the potential regulation of ROS in cardiac angiogenesis. We found: (i) following MI, gp91phox, a subunit of NADPH oxidase, a key enzyme for ROS production, was significantly increased in the border zone at day 2, followed by the infarcted myocardium at day 4, peaked at day 7 and declined at day 14, while superoxide dismutase was significantly reduced; (ii) malondialdehyde, a marker of oxidative stress, was significantly increased in the infarcted myocardium at day 7; (iii) pre-existing blood vessels in the infarcted myocardium underwent necrosis post-MI, whereas newly formed vessels appeared at the border zone at day 4, and then extended into the infarcted myocardium, where microvascular ...

Correlation between NGF/TrkA and microvascular density in human pterygium

International Journal of Experimental Pathology — Mon, 14 Sep 2009 23:00:00 +0100

Pterygium is a surface ocular lesion that is associated with chronic UV exposure. The primary effect is a solar actinic elastosis within the stroma. All the other changes are secondary. Pterygium is characterized by proliferation, inflammatory infiltrates, fibrosis, angiogenesis and extracellular matrix breakdown. The aim of this study was to correlate microvascular density and nerve growth factor (NGF)/NGF-receptor transmembrane tyrosine kinase (TrkA) expression in endothelial cells in human pterygium. Specimens of human pterygium obtained from 30 patients who had undergone surgical excision and of 10 normal bulbar conjunctiva were investigated immunohistochemically by using anti-CD31, anti-NGF and anti-TrkA antibodies. Results showed that endothelial cells in human pterygium are immunore...

A physiologically relevant atherogenic diet causes severe endothelial dysfunction within 4 weeks in rabbit

International Journal of Experimental Pathology — Mon, 14 Sep 2009 23:00:00 +0100

A physiological atherogenic human diet consists of 0.1% cholesterol, fat, as well as high levels of methionine, which is the precursor to homocysteine. The pathological effects of a diet enriched with physiologically high levels of cholesterol, methionine and fat over a short period on the aorta are unknown. In this regard, we sought to determine the effects of a 0.1% cholesterol diet in combination with a 1% methionine over a 4-week period on endothelial function and artery pathology and the expression of endothelial nitric oxide synthase as well as nitrosative stress by nitrotyrosine (NT), oxidative stress by heat shock protein 70 (HSP70) and endoplasmic reticulum stress by glucose regulated protein 78 (GRP78). Rabbits were fed for 4 weeks a diet supplemented with 1% methionine + 0.1% ch...

PDEs1-5 activity and expression in tissues of cirrhotic rats reveal a role for aortic PDE3 in NO desensitization

International Journal of Experimental Pathology — Mon, 14 Sep 2009 23:00:00 +0100

In conclusion, these changes in PDE isozymes could greatly contribute to NO desensitization and to the regulation of vascular and renal function in liver cirrhosis. (Source: International Journal of Experimental Pathology)

Gamma–delta T cell subsets are differentially associated with granuloma development and organization in a bovine model of mycobacterial disease

International Journal of Experimental Pathology — Mon, 14 Sep 2009 23:00:00 +0100

The objective of this study was to use an in vivo bovine model to evaluate [gamma][delta] T cells during the early host immune response to mycobacterial infection. We used immunofluorescent staining, hyperspectral microscopy, and computerized assisted morphometry to evaluate staining and distribution of [gamma][delta] T cells during development of organized and unorganized granulomas. Our data suggest that bovine [gamma][delta] T cell subsets are differentially recruited to early infection sites, and may be instrumental during the initial antimycobacterial host immune response as well as for granuloma organization. (Source: International Journal of Experimental Pathology)

Experimental mouse models for hepatocellular carcinoma research

International Journal of Experimental Pathology — Tue, 21 Jul 2009 11:27:36 +0100

Every year almost 500,000 new patients are diagnosed with hepatocellular carcinoma (HCC), a primary malignancy of the liver that is associated with a poor prognosis. Numerous experimental models have been developed to define the pathogenesis of HCC and to test novel drug candidates. This review analyses several mouse models useful for HCC research and points out their advantages and weaknesses. Chemically induced HCC mice models mimic the injury-fibrosis-malignancy cycle by administration of a genotoxic compound alone or, if necessary, followed by a promoting agent. Xenograft models develop HCC by implanting hepatoma cell lines in mice, either ectopically or orthotopically; these models are suitable for drug screening, although extrapolation should be considered with caution as multiple ce...

Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

The phosphodiesterase type 5 (PDE5) inhibitor, Sildenafil, is a novel, oral treatment approach for pulmonary hypertension. As Leydig cells present PDE5, this study was conducted to investigate the effects of the chronic treatment with Sildenafil (25 mg/kg) on male Swiss Webster mice steroidogenesis. After a 4-week long experimental design, Leydig cells were analysed by morphological and immunocytochemical procedures. Serum testosterone was assayed by radioimmunoassay. Leydig cells presented noteworthy ultrastructural alterations, such as a vesicular smooth endoplasmic reticulum, large vacuoles scattered through the cytoplasm, enlarged mitochondria with discontinue cristaes and whorle membranes with vesicles at the periphery, which are typical characteristics of an activated steroid-secreti...

Tumourigenic effect of Schistosoma haematobium total antigen in mammalian cells

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

Schistosoma haematobium is endemic in several regions of Africa and has been shown to be associated with predominantly squamous cell bladder carcinoma. The mechanisms underlying the association between S. haematobium and bladder squamous cell carcinoma is largely unknown. All the reports so far, demonstrate exclusively an epidemiological evidence linking S. haematobium infection with squamous cell bladder carcinoma. We hypothesized that these parasite antigens might induce tumourigenesis. For this, we used normal mammalian cells of Chinese hamster ovary (CHO) and treated the cells in culture with S. haematobium total antigen (Sh). Our results showed increased proliferation in Sh-treated cells in comparison with the controls. The CHO cells exposed to Sh were inoculated subcutaneously into m...

Microliths in the parotid of ferret investigated by electron microscopy and microanalysis

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

The present investigation is an attempt to determine the occurrence, elemental composition and formation of microliths in the parotid of ferret. Parotids from four normal ferrets were examined by electron microscopy and X-ray microanalysis. Crystalline microliths were found in phagosomes of acinar cells, which occasionally contained secretory material, and in phagosomes situated between mitochondria of striated ductal cells. Crystalline microliths and microliths that consisted of granular material either without crystals or mixed with a component of crystals were found in lumina, where they were often associated with cellular debris. The crystals contained calcium and phosphorus. Phagy and stagnation related to pockets of inefficient secretory activity have been previously found to be feat...

Human respiratory syncytial virus A2 strain replicates and induces innate immune responses by respiratory epithelia of neonatal lambs

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

This study shows that neonatal lambs can be infected with RSV strain A2 and the pulmonary pathology mimics that of RSV infection in human infants thereby making the neonatal lamb a useful animal model to study disease pathogenesis and therapeutics. RSV infection induces increased expression of surfactant proteins A and D in lambs, which may also be an important feature of infection in newborn infants. (Source: International Journal of Experimental Pathology)

Expression and localization of augmenter of liver regeneration in human muscle tissue

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

In conclusion, the present data set Alrp, as a factor associated with mitochondria also in human tissue, call for future studies aimed at establishing Alrp as an important factor involved in the molecular events that trigger neuromuscular diseases. (Source: International Journal of Experimental Pathology)

Protein expression in salivary glands of rats with streptozotocin diabetes

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

Diabetes mellitus (DM) is a widespread disease with high morbidity and health care costs. An experimental animal model was employed, using morphological and biochemical methods, to investigate the effects of DM on the expression and compartmentation of salivary gland proteins. The distribution of proline-rich proteins (PRP), submandibular mucin (Muc10) and the regulatory (RI and RII) subunits of cyclic AMP-dependent protein kinase type I and type II was determined in the parotid and submandibular (SMG) glands of rats treated with streptozotocin. Quantitative immunocytochemistry of secretory granules in diabetic glands revealed decreases of 30% for PRP in both the parotid and SMG, and a 40% decrease in Muc10 in the SMG. Immunogold labelling showed that RII decreased in nuclei and the cytopl...

Diabetes induces stromal remodelling and increase in chondroitin sulphate proteoglycans of the rat ventral prostate

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

Extracellular matrix (ECM) remodelling is an important process involved in prostate cancer progression. Alterations in ECM caused by diabetes in different tissues such as kidney is well described; however, it is poorly investigated in prostate. The aim of this study was to evaluate changes in ECM of rat prostate showing gland atrophy caused by diabetes and their implications in development of malignant lesions. Diabetes was induced in Wistar rats using alloxan (45 mg/kg bw). After 90 days of diabetes onset, animals were killed and ventral prostate was removed and prepared for light microscopy following immunoreaction for fibronectin, chondroitin sulphate and Picrossirius staining for collagen fibres. Proteoglycans (PG) were identified at transmission electron microscopy after fixation with...

Calpain-mediated breakdown of cytoskeletal proteins contributes to cholecystokinin-induced damage of rat pancreatic acini

International Journal of Experimental Pathology — Sun, 19 Jul 2009 23:00:00 +0100

The cytosolic cysteine protease calpain is implicated in a multitude of cellular functions but also plays a role in cell damage. Our previous results suggest that an activation of calpain accompanied by a decrease in its endogenous inhibitor calpastatin may contribute to pancreatic damage during cerulein-induced acute pancreatitis. The present study aimed at the time course of secretagogue-induced calpain activation and cellular substrates of the protease. Isolated rat pancreatic acini were incubated with a supramaximal concentration of cholecystokinin (0.1 [mu]M CCK) for 30 min in the presence or absence of the calpain inhibitor Z-Val-Phe methyl ester (100 [mu]M ZVP). The activation of calpain and the expression of calpastatin and the actin cytoskeleton-associated proteins [alpha]II-spect...

A critical analysis of current in vitro and in vivo angiogenesis assays

International Journal of Experimental Pathology — Fri, 15 May 2009 17:38:27 +0100

The study of angiogenesis has grown exponentially over the past 40 years with the recognition that angiogenesis is essential for numerous pathologies and, more recently, with the advent of successful drugs to inhibit angiogenesis in tumours. The main problem with angiogenesis research remains the choice of appropriate assays to evaluate the efficacy of potential new drugs and to identify potential targets within the angiogenic process. This selection is made more complex by the recognition that heterogeneity occurs, not only within the endothelial cells themselves, but also within the specific microenvironment to be studied. Thus, it is essential to choose the assay conditions and cell types that most closely resemble the angiogenic disease being studied. This is especially important when ...

Corrigendum

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

(Source: International Journal of Experimental Pathology)

Ischaemia/reperfusion induced cardiac stem cell homing to the injured myocardium by stimulating stem cell factor expression via NF-κB pathway

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

Ischaemia/reperfusion (I/R) is a major cause of heart failure. Recently cardiac stem cells (CSCs) were proposed as the most appropriate cell type for heart disease therapy. However, it is still unclear whether I/R can stimulate the CSCs homing to the injured myocardium. Male Sprague[ndash]Dawley rats were subjected to a 30-min ischaemia followed by reperfusion of different intervals. RT-PCR, western blotting and immunohistochemistry were performed to detect stem cell factor (SCF) expression at mRNA and protein levels respectively. Activation of nuclear factor-[kappa]B (NF-[kappa]B) was determined by electrophoretic mobility shift assay. To assess the homing of CSCs in vivo, BrdU-labelled CSCs were injected into AV-groove before induction of ischaemia and examined by immunofluorescent stain...

Expression of ADAM-15 in rat myocardial infarction

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

This study revealed a significant difference of ADAM-15 expression in rat MI and indicated that ADAM-15 maybe one of the important factors involved in inflammatory response and cardiac remodelling of rat MI. (Source: International Journal of Experimental Pathology)

Apoptosis and fibrosis are early features of heart failure in an animal model of metabolic cardiomyopathy

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

In previous experiments, we observed signs of cardiac failure in mice overexpressing lipoprotein lipase (LPL) under the control of a muscle specific promotor and in peroxisome proliferators activated receptor alpha (PPAR[alpha]) knockout mice overexpressing LPL under the control of the same promotor. In our current investigations, we focussed on morphological consequences and changes in mRNA and protein expression in hearts from these animals. mRNA expression was analysed by differential display analysis and Northern blot as well as by cDNA microarray analysis followed by pathway analysis. Protein expression was examined using immunoblot and immunohistochemistry. Fibrosis was determined by chromotrope aniline blue staining for collagen. A distinct increase in the expression of [alpha]-tubu...

Apoptosis, cell proliferation and modulation of cyclin-dependent kinase inhibitor p21cip1 in vascular remodelling during vein arterialization in the rat

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

Neo-intima development and atherosclerosis limit long-term vein graft use for revascularization of ischaemic tissues. Using a rat model, which is technically less challenging than smaller rodents, we provide evidence that the temporal morphological, cellular, and key molecular events during vein arterialization resemble the human vein graft adaptation. Right jugular vein was surgically connected to carotid artery and observed up to 90 days. Morphometry demonstrated gradual thickening of the medial layer and important formation of neo-intima with deposition of smooth muscle cells (SMC) in the subendothelial layer from day 7 onwards. Transmission electron microscopy showed that SMCs switch from the contractile to synthetic phenotype on day 3 and new elastic lamellae formation occurs from day...

A detailed microscopic study of the changes in the aorta of experimental model of postmenopausal rats fed with repeatedly heated palm oil

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

This study was performed to examine the effects of repeatedly heated palm oil mixed with 2% cholesterol diet on atherosclerosis in oestrogen-deficient postmenopausal rats. Ovariectomy causes disruption of tunica intima layer of the rat aorta simulating a postmenopausal condition in females. Twenty-four ovariectomized female Sprague[ndash]Dawley rats were divided into four groups. The control group received 2% cholesterol diet without palm oil. A diet with 2% cholesterol content fortified with fresh, once-heated and five-times-heated palm oil was given to the other treatment groups. The rats were sacrificed at the end of 4 months of study and the aortic arch tissue was processed for histomorphometry and electron microscopy. On observation, there was disruption of the intimal layer of the ov...

High dietary methionine plus cholesterol stimulates early atherosclerosis and late fibrous cap development which is associated with a decrease in GRP78 positive plaque cells

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

The role of homocysteine, or its precursor methionine, in the formation of fibrous caps and its association with endoplasmic reticulum (ER) stress is unclear. Homocysteine can stimulate collagen accumulation and upregulate the ER stress chaperone glucose regulated protein 78 (GRP78). The aim of this study was to determine if high dietary methionine would increase fibrous caps, and that removal of an atherogenic diet would decrease the amount of ER stressed cells. New Zealand white rabbits were fed for 2, 4, or 12 weeks an atherogenic diet [1% methionine + 0.5% cholesterol (2MC, 4MC or 12MC)]; for 4 or 12 weeks a 0.5% cholesterol diet (4Ch, 12Ch); and to study plaque regression, an MC diet for 2 or 4 weeks accompanied by 10 weeks of a normal diet (2MCr, 4MCr). Endothelial function, atherosc...

Can eccentric arterial plaques alone cause flow stagnation points and favour thrombus incorporation?

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

We have used an experimental model of aorta stenosis, with a Plexiglas plug, simulating a stable atheromatous plaque that promotes local turbulence and thrombosis. With animal survival of more than 24 h, we followed the partial fibrinolysis of the thrombus as well as its posterior organization and incorporation to the arterial wall as a neointima for up to 30 days. The mushroom plug form permitted the development of recirculation and stasis areas around it, favouring this evolution. Despite noted limitations, this study demonstrates that thrombus incorporation can contribute to plaque extension, as it can promote recirculation and stasis areas. (Source: International Journal of Experimental Pathology)

Microtubule depolymerizing vascular disrupting agents: novel therapeutic agents for oncology and other pathologies

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

Vascular disrupting agents (VDAs) are a relatively new group of 'vascular targeting' agents that exhibit selective activity against established tumour vascular networks, causing severe interruption of tumour blood flow and necrosis to the tumour mass. Microtubule depolymerizing agents form by far the largest group of small molecular weight VDAs many of which, including lead compound disodium combretastatin A-4 3-O-phosphate (CA-4-P), are under clinical development for cancer. Although distinct from the angiogenesis inhibitors, VDAs can also interfere with angiogenesis and therefore constitute a potential group of novel drugs for the treatment of pathological conditions characterized by excessive angiogenesis, in addition to cancer. The endothelial cytoskeleton is the primary cellular targe...

Coronary vessel development and insight towards neovascular therapy

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

Formation of the coronary arteries consists of a precisely orchestrated series of morphogenetic and molecular events which can be divided into three distinct processes: vasculogenesis, angiogenesis and arteriogenesis (Risau 1997; Carmeliet 2000). Even subtle perturbations in this process may lead to congenital coronary artery anomalies, as occur in 0.2[ndash]1.2% of the general population (von Kodolitsch et al. 2004). Contrary to the previously held dogma, the process of vasculogenesis is not limited to prenatal development. Both vasculogenesis and angiogenesis are now known to actively occur within the adult heart. When the need for regeneration arises, for example in the setting of coronary artery disease, a reactivation of embryonic processes ensues, redeploying many of the same molecul...

The vasculature in rheumatoid arthritis: cause or consequence?

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

The expansion of the synovial lining of joints in rheumatoid arthritis (RA) necessitates an increase in the vascular supply to the synovium, to cope with the increased requirement for oxygen and nutrients. New blood vessel formation [ndash]'angiogenesis'[ndash] is recognized as a key event in the formation and maintenance of the pannus in RA, suggesting that targeting blood vessels in RA may be an effective future therapeutic strategy. Although many pro-angiogenic factors have been demonstrated to be expressed in RA synovium, vascular endothelial growth factor (VEGF) has been demonstrated to a have a central involvement in the angiogenic process in RA. Nevertheless, it is unclear whether angiogenesis [ndash] whether driven by VEGF and/or other factors [ndash] should be considered as a 'cau...

Angiogenesis drives psoriasis pathogenesis

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

Psoriasis pathogenesis is closely associated with disease-inducing Th1 and Th17 cells. Yet, several studies suggest that aberrant keratinocyte or endothelial cell signalling significantly contributes to disease manifestation. Histological hallmarks of psoriatic skin include the infiltration of multiple immune cells, keratinocyte proliferation and increased dermal vascularity. Formation of new blood vessels starts with early psoriatic changes and disappears with disease clearance. Several angiogenic mediators like vascular endothelial growth factor, hypoxia-inducible factors, angiopoietins and pro-angiogenic cytokines, such as tumour necrosis factor (TNF), interleukin (IL)-8 and IL-17, are up-regulated in psoriasis development. Contact- and mediator-dependent factors derived from keratinocy...

Neutrophils: key mediators of tumour angiogenesis

International Journal of Experimental Pathology — Mon, 11 May 2009 04:00:00 +0100

It is now well known that most malignant tumours contain a significant amount of leucocytic infiltrates the presence of which has, on many occasions, been linked to poor patient prognosis. These leucocyte populations are recruited to tumours by chemotactic factors released by either viable or necrotic tumour cells, or by cells within the tumour stroma. In recent times, most studies have analysed the role that tumour-associated macrophages (TAM) have on tumour progression. However, there is now increasing evidence to show that neutrophils also actively participate in this process. Whilst there are some data to suggest that neutrophil-derived factors can promote genetic mutations leading to tumourigenesis, or secrete factors that promote tumour cell proliferation; there is now substantial ev...

Back to basics – how the evolution of the extracellular matrix underpinned vertebrate evolution

International Journal of Experimental Pathology — Sat, 21 Mar 2009 11:11:01 +0100

The extracellular matrix (ECM) is a complex substrate that is involved in and influences a spectrum of behaviours such as growth and differentiation and is the basis for the structure of tissues. Although a characteristic of all metazoans, the ECM has elaborated into a variety of tissues unique to vertebrates, such as bone, tendon and cartilage. Here we review recent advances in our understanding of the molecular evolution of the ECM. Furthermore, we demonstrate that ECM genes represent a pivotal family of proteins the evolution of which appears to have played an important role in the evolution of vertebrates. (Source: International Journal of Experimental Pathology)

Cartilage metabolism and cell based therapiesfor tissue regeneration

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

(Source: International Journal of Experimental Pathology)

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International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

(Source: International Journal of Experimental Pathology)

The molecular and cellular basis of exostosis formation in hereditary multiple exostoses

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

(Source: International Journal of Experimental Pathology)

Histological characteristics of human papilloma-virus-positive and -negative invasive and in situ squamous cell tumours of the penis

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

In conclusion, about half of invasive penile squamous carcinomas in this study were hrHPV-positive, most notably to HPV 16, and probably arose through in situ lesions whereas the other half of invasive penile lesions appeared to be unrelated to hrHPV. A number of histological characteristics differed significantly between hrHPV-positive and -negative invasive penile carcinomas. (Source: International Journal of Experimental Pathology)

Evaluation of a magnetic resonance biomarker of osteoarthritis disease progression: doxycycline slows tibial cartilage loss in the Dunkin Hartley guinea pig

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

The objective was to assess the effect of doxycycline treatment on a magnetic resonance imaging (MRI) biomarker of cartilage volume loss, and on matrix metalloproteinase (MMP) activity in a guinea pig osteoarthritis model. Guinea pigs (9 months old) were dosed with vehicle or doxycycline, 0.6, 3.0 mg/kg/day for 66 days. Fat-suppressed 3D gradient-echo MRI of the left knee was acquired pre- and post dosing. Change in medial tibial plateau (MTP) cartilage volume (MT.VC) was determined using image analysis. At termination, MTP cartilage was removed from knees and proteolytic MMP activity determined using a fluorescent peptide substrate assay. Vehicle-treated animals lost 20.5% (95% CI mean 25.6[ndash]15.1) MT.VC. The doxycycline (0.6 mg/kg/day) group lost 8.6% (P < 0.05, 95% CI 20.6 to [minus...

The lectin KM+ induces corneal epithelial wound healing in rabbits

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

This study aims at investigating a possible effect of KM+ on corneal regeneration in rabbits. A 6.0-mm diameter area of debridement was created on the cornea of both eyes by mechanical scraping. The experimental eyes received drops of KM+ (2.5 [mu]g/ml) every 2 h. The control eyes received buffer. The epithelial wounded areas of the lectin-treated and untreated eyes were stained with fluorescein, photographed and measured. The animals were killed 12 h (group 1, n = 5), 24 h (group 2, n = 10) and 48 h (group 3, n = 5) after the scraping. The corneas were analysed histologically (haematoxylin and eosin and immunostaining for proliferation cell nuclear antigen, p63, vascular endothelial growth factor, c-Met and laminin). No significant differences were found at the epithelial gap between trea...

Dystroglycan patterns on the prostate of non-obese diabetic mice submitted to glycaemic control

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

Dystroglycan (DG) is an adhesion protein which plays a crucial role in the maintenance of tissue integrity. Diabetes has been pointed out as a disease which causes harmful effects on prostate function. Therefore, the main objective of this work was to verify DG distribution and structure features in diabetic mice with and without glycaemic control and to relate these parameters to prostate pathogenesis. Thirty mice (Nod and BALB/c) were divided into three groups after 20 days of diabetic state: the control group received a 5 ml/kg dose of physiological saline daily for 20 days; the diabetic group had the same treatment as the control group; the diabetic-insulin group received 4[ndash]5 IU doses of Neutral Protamine Hagedorn (NPH) insulin daily for 20 days. After 20 days of treatment, all a...

Reduced expression of IL-3 mediates intestinal mast cell depletion in diabetic rats: role of insulin and glucocorticoid hormones

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

In conclusion, our findings show that there is a causative relationship between down-regulation of mast cell numbers and the expression of IL-3 associated with diabetic state. In addition, as both parameters were suppressed by administration of insulin and RU 486, it indicates that an imbalance between the systemic levels of insulin and glucocorticoid hormones seems to be implicated in the reduction in intestinal mast cell population and refractoriness to antigen provocation in alloxan diabetes. (Source: International Journal of Experimental Pathology)

The serum D-xylose test as a useful tool to identify malabsorption in rats with antigen specific gut inflammatory reaction

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

In conclusion, the use of serum d-xylose test was useful to identify the presence of small intestinal malabsorption in our antigen specific gut inflammatory reaction rat model. (Source: International Journal of Experimental Pathology)

Anti-inflammatory effects of the genus Bifidobacterium on macrophages by modification of phospho-IκB and SOCS gene expression

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

In this study, we investigated how three species of Bifidobacterium and Enterococcus faecalis differentially modulate production of cytokines from lipopolysaccharide (LPS)-stimulated macrophages in vitro using RAW264.7 cells. The mRNA levels of proinflammatory cytokines were remarkably increased after exposure to LPS, E. faecalis alone and LPS combined with E. faecalis. In contrast, IL-10 mRNA levels were significantly decreased after exposure to E. faecalis compared with exposure to Bifidobacterium species. When cells were exposed to Bifidobacterium species combined with LPS, mRNA levels of IL12p40 were decreased by co-culture with B. breve and B. longum, IL-1[beta] mRNA levels were decreased by B. breve and B. adorescentis and TNF-[alpha] mRNA levels were decreased by B. adolescentis com...

Characterization of cerebral malaria in the outbred Swiss Webster mouse infected by Plasmodium berghei ANKA

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

Plasmodium berghei ANKA (PbA) infection in susceptible inbred mouse strains is the most commonly used experimental model to study pathogenesis of cerebral malaria (CM). Indeed, many concepts on mechanisms related to this complication have arisen from works using this model. Although inbred strains present several advantages and are indicated for most studies, the use of outbred models can show unique usefulness in a number of approaches such as fine post-quantitative trait loci mapping and discovery of genes relevant to CM susceptibility or resistance, as well as pharmacological and vaccine studies. Here we describe the features of PbA infection and CM incidence, and characterize the associated multiorgan pathology in the outbred Swiss Webster mouse. This model showed a sizeable (62.7%) an...

Establishment of lethal inhalational infection with Francisella tularensis (tularaemia) in the common marmoset (Callithrix jacchus)

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

Susceptibility and lethality studies of inhalational tularaemia were undertaken using the common marmoset (Callithrix jacchus) to determine its suitability as a non-human primate model. Pairs of marmosets were exposed to varying challenge doses of Francisella tularensis by the airborne route and monitored for up to 14 days postchallenge (p.c.). Lethal infection was achieved following a retained dose of less than 10 bacterial colony-forming units (CFU). However, precise LD50 determination was not possible. The model was characterized using a target challenge dose of approximately 100 CFU. Increased core body temperature was the first indicator of disease, at approximately 2.5 days p.c. Overt clinical signs were first observed 12[ndash]18 h after the temperature increase. Significantly decre...

Megaoesophagus in Rassf1a-null mice

International Journal of Experimental Pathology — Wed, 18 Mar 2009 04:00:00 +0100

In this report, we show that aged Rassf1a-null mice have an enhanced susceptibility to megaoesophagus compared with wild-type littermates ([sim]20%vs. [sim]2% incidence respectively; P = 0.01). Histological examination of the dilated oesophaguses shows a reduction in the numbers of nerve cells (both ganglia and nerve fibres) in the myenteric plexus of the dilated mid and lower oesophagus that was confirmed by S100 immunohistochemistry. There was also a chronic inflammatory infiltrate and subsequent fibrosis of the myenteric plexus and the muscle layers. These appearances closely mimic the gross and histopathological findings in human cases of megaoesophagus/achalasia, thus demonstrating that this is a representative mouse model of the disease. Thus, we have identified a genetic cause of th...

Involvement of the cytoskeletal elements in articular cartilage homeostasis and pathology

International Journal of Experimental Pathology — Tue, 20 Jan 2009 03:25:44 +0100

The cytoskeleton of all cells is a three-dimensional network comprising actin microfilaments, tubulin microtubules and intermediate filaments. Studies in many cell types have indicated roles for these cytoskeletal proteins in many diverse cellular processes including alteration of cell shape, movement of organelles, migration, endocytosis, secretion, cell division and extracellular matrix assembly. The cytoskeletal networks are highly organized in structure enabling them to fulfil their biological functions. This review will primarily focus on the organization and function of the three major cytoskeletal networks in articular cartilage chondrocytes. Articular cartilage is a major load-bearing tissue of the synovial joint; it is well known that the cytoskeleton acts as a physical interface ...

Past, Present and Future Insights into the Understanding and Treatment of Osteoarthritis: Molecular and Mechanical Approaches

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

(Source: International Journal of Experimental Pathology)

Long-term inhibition of 5-alpha reductase and aromatase changes the cellular and extracellular compartments in gerbil ventral prostate at different postnatal ages

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

In conclusion, the inhibition of 5[alpha]-r and Aro enzymes affected, in a persistent manner, the structural and ultrastructural morphology of the prostate, irrespective of the gerbil's age. Hence these enzymes appear to be crucial in the maintenance of this gland during postnatal development. Also, these data bring more light to the complex issue of the mechanisms of local steroid metabolism and prostatic histology. Thus, the blockade of the steroid-metabolizing enzymes provided an important novel tool to study the relationship between sex steroids and normal physiology and diseases of the prostate. (Source: International Journal of Experimental Pathology)

Identification of monoclinic calcium pyrophosphate dihydrate and hydroxyapatite in human sclera using Raman microspectroscopy

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

Raman microspectroscopy was first used to determine the composition of a calcified plaque located at the pterygium-excision site of a 51-year-old female patient's left nasal sclera after surgery. It was unexpectedly found that the Raman spectrum of the calcified sample at 1149, 1108, 1049, 756, 517, 376 and 352/cm was similar to the Raman spectrum of monoclinic form of calcium pyrophosphate dihydrate (CPPD) crystal, but differed from the Raman spectrum of triclinic form of CPPD. An additional peak at 958/cm was also observed in the Raman spectrum of the calcified plaque, which was identical to the characteristic peak at 958/cm of hydroxyapatite (HA). This is the first study to report the spectral biodiagnosis of both monoclinic CPPD and HA co-deposited in the calcified plaque of a patient ...

Histopathological abnormalities of prolonged alloxan-induced diabetes mellitus in rabbits

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

The objective of this study was to investigate the prolonged complications of untreated diabetes on histomorphology of rabbits. Diabetes mellitus was experimentally induced in one group of New Zealand white male rabbits by intraperitoneal administration of four doses of alloxan @ 80 mg/kg b.w. at weekly intervals following 12 h fasting. Other group of rabbits served as healthy controls that received isotonic saline in a similar manner. The establishment of diabetes mellitus was confirmed by fasting blood glucose levels. For histomorphological study of different organs, 50% of the animals were killed after 7 weeks and the rest after 26 weeks. Routine haematoxylin and eosin stain and Gomori's modified stain were used. The blood glucose level of diabetic rabbits increased significantly throug...

Does diclofenac increase the risk of cervical necrotizing fasciitis in a rat model?

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

This study suggests that diclofenac does not seem to increase the risk of occurrence of CNF. Nonetheless, NSAIDs can mask inflammatory signs of an already spreading CNF. (Source: International Journal of Experimental Pathology)

Trypanosoma cruziand myoid cells from seminiferous tubules: interaction and relation with fibrous components of extracellular matrix in experimental Chagas' disease

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

The main transmission route of Trypanosoma cruzi is by triatomine bugs. However, T. cruzi is also transmitted through blood transfusion, organ transplantation, ingestion of contaminated food or fluids, or is congenital. Sexual transmission, although suggested since the discovery of Chagas' disease, has remained unproven. Sexual transmission would require T. cruzi to be located at the testes and ovaries. Here we investigated whether T. cruzi is present in the gonads of mice infected with 104T. cruzi trypomastigotes from the CL strain. Fourteen days after experimental infection, histopathological examination showed alterations in the extracellular matrix of the lamina propria of the seminiferous tubules. Furthermore, amastigotes were present in seminiferous tubules, within myoid cells, and i...

Polymorphonuclear cells stimulate the migration and metastatic potential of rat sarcoma cells

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

The tumour microenvironment, which is largely composed of inflammatory cells, is a crucial participant in the neoplastic process through the promotion of cell proliferation, survival and migration. Neutrophil polymorphonuclear cells (PMNs) induce inflammatory reactions that can be either cytotoxic for tumour cells or can promote tumour growth and metastasis. Previously, we have reported a spontaneous metastasis tumour model that has tumour PMNs infiltration, and metastasis, to liver and spleen. The aim of this study was to evaluate the PMNs influences on the tumour cell invasion and metastatic properties. We analysed intercellular adhesion molecule-1 (ICAM-1), urokinase-type plasminogen activator receptor (uPAR), MT1-MMP (membrane type 1-matrix metalloproteinase) and MMP2 protein expressio...

Delayed local inflammatory response induced by Thalassophryne nattereri venom is related to extracellular matrix degradation

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

In this study, we analysed the movement of leucocytes after T. nattereri venom injection in the intraplantar region of Swiss mice, the production of pro-inflammatory mediators and the venom potential to elicit matrix metalloproteinase production and extracellular matrix degradation. Total absence of mononuclear and neutrophil influx was observed until 14 days, but the venom stimulates pro-inflammatory mediator secretion. Matrix metalloproteinases (MMP)-2 and MMP-9 were detected in greater quantities, accompanied by tissue degradation of collagenous fibre. An influx of mononuclear cells was noted very late and at this time the levels of IL-6, IL-1[beta] and MMP-2 remained high. Additionally, the action of venom on the cytoskeletal organization was assessed in vitro. Swift F-actin disruption...

Morphological parameters for assessment of burn severity in an acute burn injury rat model

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

This study confirms key morphological parameters can be an important tool for the detection of burn severity in this acute burn model. (Source: International Journal of Experimental Pathology)

Experimental acute respiratory Burkholderia pseudomallei infection in BALB/c mice

International Journal of Experimental Pathology — Mon, 19 Jan 2009 05:00:00 +0100

The objective of this study was to establish and characterise a relevant, acute respiratory Burkholderia pseudomallei infection in BALB/c mice. Mice were infected with 100 B. pseudomallei strain BRI bacteria by the aerosol route (approximately 20 median lethal doses). Bacterial counts within lung, liver, spleen, brain, kidney and blood over 5 days were determined and histopathological and immunocytochemical profiles were assessed. Bacterial numbers in the lungs reached approximately 108 cfu/ml at day 5 post-infection. Bacterial numbers in other tissues were lower, reaching between 103 and 105 cfu/ml at day 4. Blood counts remained relatively constant at approximately 1.0 × 102 cfu/ml. Foci of acute inflammation and necrosis were seen within lungs, liver and spleen. These results suggest t...

Expression of inhibitors of apoptosis family protein in 7,12-dimethylbenz[a]anthracene-induced hamster buccal-pouch squamous-cell carcinogenesis is associated with mutant p53 accumulation and epigenetic changes

International Journal of Experimental Pathology — Sun, 13 Jul 2008 04:00:00 +0100

Fifty outbred Syrian golden hamsters were equally divided into three experimental groups and two control groups. The pouches of the experimental groups were painted bilaterally with a 0.5% 7,12-dimethylbenz[a]anthracene (DMBA) solution thrice a week for 3, 7 and 14 weeks. One of the control groups was applied with mineral oil while another control group remained untreated throughout the experiment. Neither survivin nor cIAP2 could be detected in any of the control tissues, whereas survivin and cIAP2 were found to be significantly increased in 3-, 7- and 14-week DMBA-treated pouches compared with the control pouches. Expression of XIAP, cIAP1 and NAIP were noted for both the control and 3-, 7- and 14-week DMBA-treated pouches, but levels were found to be significantly elevated in the experi...

Superficial exudates of neutrophils prevent invasion of Bacillus anthracis bacilli into abraded skin of resistant mice

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:36 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page 180-187, June 2008. SummarySkin window procedures in humans have shown rapid accumulation of neutrophils into the exuded fluids above abraded skin. The present study was undertaken to determine if similar epicutaneous neutrophil accumulation might explain the extreme ... (Source: International Journal of Experimental Pathology)

Down-regulation of MyoD gene expression in rat diaphragm muscle with heart failure

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:13 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page 216-222, June 2008. SummaryDiaphragm myopathy has been described in patients with heart failure (HF), with alterations in myosin heavy chains (MHC) expression. The pathways that regulate MHC expression during HF have not been described, and myogenic regulatory factors (MRFs) ... (Source: International Journal of Experimental Pathology)

Antibody titres to heat shock protein 27 are elevated in patients with acute coronary syndrome

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:13 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page 209-215, June 2008. SummaryIgG antibody titres to heat shock protein 27 (anti-Hsp27) were measured to determine whether these titres were affected in patients admitted with acute coronary syndrome. Blood samples were taken from 94 patients admitted with acute coronary ... (Source: International Journal of Experimental Pathology)

BSMB Autumn 2007 Meeting Report

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:12 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page A1-A29, June 2008. (Source: International Journal of Experimental Pathology)

Inhalation of Stachybotrys chartarum causes pulmonary arterial hypertension in mice

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:12 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page 201-208, June 2008. SummaryInhalation of Stachybotrys chartarum, a ubiquitous fungus in our living environment, has been suspected as a cause of acute idiopathic pulmonary haemorrhage in infants, but its relation to human diseases is not yet known. The aim of present study ... (Source: International Journal of Experimental Pathology)

Experimental respiratory anthrax infection in the common marmoset (Callithrix jacchus)

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:12 +0100

International Journal of Experimental Pathology, Volume 89, Issue 3, Page 171-179, June 2008. SummaryInhalational anthrax is a rare but potentially fatal infection in man. The common marmoset (Callithrix jacchus) was evaluated as a small non-human primate (NHP) model of inhalational anthrax infection, as an alternative to larger NHP species. The ... (Source: International Journal of Experimental Pathology)

Acute uriticaria-like lesions in allergen-unexposed cutaneous tissues in a mouse model of late allergic rhinitis

International Journal of Experimental Pathology — Tue, 06 May 2008 08:20:10 +0100

This study examined the development of cutaneous lesions in a mouse model of late allergic rhinitis (LAR). BALB/c mice were sensitized by ovalbumin (OVA) ... (Source: International Journal of Experimental Pathology)

The molecular and cellular basis of exostosis formation in hereditary multiple exostoses

International Journal of Experimental Pathology — Thu, 01 May 2008 18:42:11 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryThe different clinical entities of osteochondromas, hereditary multiple exostoses (HME) and non-familial solitary exostosis, are known to express localized exostoses in their joint metaphyseal cartilage. In the current study biopsies of ... (Source: International Journal of Experimental Pathology)

Increased pancreatic islet mass is accompanied by activation of the insulin receptor substrate-2/serine-threonine kinase pathway and augmented cyclin D2 protein levels in insulin-resistant rats

International Journal of Experimental Pathology — Mon, 21 Apr 2008 18:27:40 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryIt is well known that glucocorticoids induce peripheral insulin resistance in rodents and humans. Here, we investigated the structural and ultrastructural modifications, as well as the proteins involved in beta-cell function and proliferation, in ... (Source: International Journal of Experimental Pathology)

Endothelial nitric oxide synthase is a critical factor in experimental liver fibrosis

International Journal of Experimental Pathology — Mon, 21 Apr 2008 17:12:10 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryReduced expression of endothelial nitric oxide synthase (eNOS) in chronic liver disease can reduce hepatic perfusion and accelerate fibrosis. The relationship between eNOS expression and liver fibrogenesis remains unclear. We investigated whether l-... (Source: International Journal of Experimental Pathology)

Expression of inhibitors of apoptosis family protein in 7,12-dimethylbenz[a]anthracene-induced hamster buccal-pouch squamous-cell carcinogenesis is associated with mutant p53 accumulation and epigenetic changes

International Journal of Experimental Pathology — Thu, 17 Apr 2008 18:24:10 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryFifty outbred Syrian golden hamsters were equally divided into three experimental groups and two control groups. The pouches of the experimental groups were painted bilaterally with a 0.5% 7,12-dimethylbenz[a]anthracene (DMBA) solution thrice a ... (Source: International Journal of Experimental Pathology)

Elevated activity of the oxidative and non-oxidative pentose phosphate pathway in (pre)neoplastic lesions in rat liver

International Journal of Experimental Pathology — Thu, 17 Apr 2008 08:38:54 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. Summary(Pre)neoplastic lesions in livers of rats induced by diethylnitrosamine are characterized by elevated activity of the first irreversible enzyme of the oxidative branch of the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PD),... (Source: International Journal of Experimental Pathology)

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

International Journal of Experimental Pathology — Thu, 17 Apr 2008 08:38:54 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryThioacetamide (TAA) has been used extensively in the development of animal models of acute liver injury. Frequently, TAA is administered intraperitoneally to induce liver damage under anaesthesia. However, it is rarely administered by intravenous ... (Source: International Journal of Experimental Pathology)

Bone repair in mandibular body osteotomy after using 2.0 miniplate system – histological and histometric analysis in dogs

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:51:29 +0100

The objective of this study was to evaluate the bone repair along a mandibular body osteotomy after using a 2.0 miniplate system. Nine adult mongrel dogs were subjected to unilateral continuous defect through an osteotomy between the mandibular 3rd ... (Source: International Journal of Experimental Pathology)

Serum FLT-3 ligand in a busulphan-induced model of chronic bone marrow hypoplasia in the female CD-1 mouse

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:51:21 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 159-170, April 2008. SummaryThe concentration of the cytokine fms-like tyrosine kinase-3 ligand (FL) is elevated in the plasma of patients treated with chemotherapy or radiotherapy for malignant conditions. In addition, plasma FL is increased in patients with bone marrow ... (Source: International Journal of Experimental Pathology)

Dysregulation of matrix metalloproteinases and their tissue inhibitors is related to abnormality of left ventricular geometry and function in streptozotocin-induced diabetic minipigs

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:51:18 +0100

This study aimed to characterize matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in relation to changes in left ventricle (LV) geometry and function in a porcine model with streptozotocin (STZ)-induced diabetes. In 15 Chinese ... (Source: International Journal of Experimental Pathology)

The haemotoxicity of azathioprine in repeat dose studies in the female CD-1 mouse

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:50:52 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 138-158, April 2008. SummaryAzathioprine (AZA) is a cytotoxic immunosuppressive drug used in the prevention of rejection in organ transplants and the treatment of auto-immune diseases. However, AZA is haemotoxic causing significant bone marrow depression. The present studies ... (Source: International Journal of Experimental Pathology)

Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:50:45 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 106-116, April 2008. SummaryExtracellular matrix (ECM) proteins are important modulators of migration, differentiation and proliferation for the various cell types present in the lungs; they influence the immune response as well as participate in the adherence of several ... (Source: International Journal of Experimental Pathology)

Secretion of collagen I and tenascin is modulated by laminin-111 in 3D culture of human adenoid cystic carcinoma cells

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:50:36 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 98-105, April 2008. SummaryAdenoid cystic carcinoma is a frequent malignant salivary gland neoplasm with high levels of recurrence and metastasis. This neoplasm expresses prominent extracellular matrix (ECM). We are studying regulatory mechanisms underlying secretion of ECM ... (Source: International Journal of Experimental Pathology)

Enhanced proliferation of aortal smooth muscle cells treated by 1,25(OH)2D3 in vitro coincides with impaired formation of elastic fibres

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:50:29 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 117-124, April 2008. SummaryElastin is the major extracellular matrix component synthesized, secreted and deposited by vascular smooth muscle cells (SMCs) in the arterial media and thus plays an important role in vascular homeostasis. Results of our previous studies showed ... (Source: International Journal of Experimental Pathology)

Cleavage and conformational changes of tau protein follow phosphorylation during Alzheimer’s disease

International Journal of Experimental Pathology — Tue, 11 Mar 2008 01:50:20 +0100

International Journal of Experimental Pathology, Volume 89, Issue 2, Page 81-90, April 2008. SummaryPhosphorylation, cleavage and conformational changes in tau protein all play pivotal roles during Alzheimer’s disease (AD). In an effort to determine the chronological sequence of these changes, in this study, using confocal microscopy, we compared ... (Source: International Journal of Experimental Pathology)

Turbulent blood flow plays an essential localizing role in the development of atherosclerotic lesions in experimentally induced hypercholesterolaemia in rats

International Journal of Experimental Pathology — Wed, 09 Jan 2008 17:43:05 +0100

International Journal of Experimental Pathology, Volume 89, Issue 1, Page 72-80, February 2008. SummaryTaking into account that atherosclerosis is a focal disease and high levels of plasma cholesterol are closely correlated with its pathogenesis, it is a challenge to explain how equal concentrations of cholesterol bathing the endothelium can produce ... (Source: International Journal of Experimental Pathology)

In vivo models for early development of colorectal liver metastasis

International Journal of Experimental Pathology — Tue, 11 Dec 2007 19:07:56 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, OnlineEarly Articles. SummaryIn Europe, colorectal cancer is the second most prevalent form of cancer diagnosed. Globally each year, almost one million cases of colorectal cancer are registered and almost half a million deaths are attributed to this disease. This high ... (Source: International Journal of Experimental Pathology)

Matrix Turnover – Mechanisms and Common Denominators: A Joint British Society for Matrix Biology/Biochemical Society Focused Meeting

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:46:19 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page A75-A97, December 2007. (Source: International Journal of Experimental Pathology)

Prenatal administration of vitamin A alters pulmonary and plasma levels of vascular endothelial growth factor in the developing mouse

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:45:56 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page 393-401, December 2007. SummaryVitamin A and the retinoids play a unique role in mammalian embryonic and foetal development and are essential for both cellular differentiation and the establishment of normal morphogenesis. Vascular endothelial growth factor (VEGF) is a known ... (Source: International Journal of Experimental Pathology)

International course on Laboratory Animal Science - Utrecht, The Netherlands July 7-18, 2008

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:45:55 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page 445, December 2007. (Source: International Journal of Experimental Pathology)

Laser capture microdissection reveals dose–response of gene expression in situ consequent to asbestos exposure

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:45:55 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page 415-425, December 2007. SummaryThe genes that mediate fibroproliferative lung disease remain to be defined. Prior studies from our laboratory showed by in situ hybridization and immunohistochemistry that the genes coding for tumour necrosis factor alpha, transforming growth ... (Source: International Journal of Experimental Pathology)

Pathology and virus dispersion in cynomolgus monkeys experimentally infected with severe acute respiratory syndrome coronavirus via different inoculation routes

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:45:55 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page 403-414, December 2007. SummarySevere acute respiratory syndrome-associated coronavirus (SARS-CoV) causes SARS. The pathogenic mechanisms of SARS-CoV remain poorly understood. Six cynomolgus monkeys were inoculated with the HKU39849 isolate of SARS-CoV via four routes. After ... (Source: International Journal of Experimental Pathology)

Toll-like receptor and tumour necrosis factor dependent endotoxin-induced acute lung injury

International Journal of Experimental Pathology — Thu, 22 Nov 2007 16:45:55 +0100

International Journal of Experimental Pathology, Volume 88, Issue 6, Page 387-391, December 2007. SummaryRecent studies on endotoxin/lipopolysaccharide (LPS)-induced acute inflammatory response in the lung are reviewed. The acute airway inflammatory response to inhaled endotoxin is mediated through Toll-like receptor 4 (TLR4) and CD14 signalling as ... (Source: International Journal of Experimental Pathology)

What is OnlineEarly?

International Journal of Experimental Pathology — Wed, 14 Nov 2007 19:28:43 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, -Not available-. What is OnlineEarly? OnlineEarly is a Blackwell Synergy service where fully corrected, fully web-functional and complete articles are published online as and when they are ready, prior to their ultimate inclusion in a print issue. This service is a ... (Source: International Journal of Experimental Pathology)

Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background

International Journal of Experimental Pathology — Tue, 13 Nov 2007 20:51:56 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, -Not available-. SummaryEscherichia coli Nissle 1917 (EcN) is a well-characterized probiotic bacterium. Although genomic comparisons of EcN with the uropathogenic E. coli strain CFT073 revealed high degrees of similarity, EcN is generally considered a non-pathogenic ... (Source: International Journal of Experimental Pathology)

Role of p38 mitogen-activated protein kinase pathway on heart failure in the infant rat after burn injury

International Journal of Experimental Pathology — Tue, 13 Nov 2007 15:59:32 +0100

We examined the hypothesis that post-burn activation of the p38 mitogen-activated protein kinase (MAPK) pathway is one aspect of the signalling cascade culminating in post-burn secretion of tumour necrosis factor (TNF)-α which contributes to post-... (Source: International Journal of Experimental Pathology)

Comparison of metastatic brain tumour models using three different methods: the morphological role of the pia mater

International Journal of Experimental Pathology — Mon, 12 Nov 2007 20:27:29 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, -Not available-. SummaryAs methods of cancer diagnosis and treatment progress, interest in metastatic brain tumours continues to increase. There are many studies using various methods of animal model and we considered that each model reflects different pathological ... (Source: International Journal of Experimental Pathology)

Oestrogen supplementation following castration promotes stromal remodelling and histopathological alterations in the Mongolian gerbil ventral prostate

International Journal of Experimental Pathology — Mon, 12 Nov 2007 20:27:29 +0100

International Journal of Experimental Pathology, Volume 0, Issue 0, Page ???, -Not available-. SummaryThe effect of oestradiol on the intact and castrated adult gerbil prostate was evaluated by focussing on stromal and epithelial disorders, and hormonal receptor immunoreactivity. The experimental animals were studied by histological, histochemical ... (Source: International Journal of Experimental Pathology)

Age-related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone-related disorders

International Journal of Experimental Pathology — Mon, 27 Aug 2007 18:34:48 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryThe Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia Spontaneous neoplasia develops in the older animals. Thirty gerbils (age 18 months... (Source: International Journal of Experimental Pathology)

Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammation

International Journal of Experimental Pathology — Mon, 27 Aug 2007 12:37:17 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryThe aim of our study was to evaluate the differences in protein and amino acid metabolism after subcutaneous turpentine administration in the soleus muscle (SOL), predominantly composed of red fibres, and the extensor digitorum longus muscle (EDL) c... (Source: International Journal of Experimental Pathology)

Matrix metalloproteinase-2, -9 and -13 are involved in fibronectin degradation of rat lung granulomatous fibrosis caused by Angiostrongylus cantonensis

International Journal of Experimental Pathology — Mon, 27 Aug 2007 12:37:16 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryPulmonary granuloma formation and fibrosis were experimentally induced in Sprague–Dawley strain rats by Angiostrongylus cantonensis. Increased protein levels of matrix metalloproteinase (MMP)-2, -9, -13 and the imbalance between these enzymes and me... (Source: International Journal of Experimental Pathology)

British Society for Matrix Biology Meeting, Autumn 2006

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:59 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page A46-A74, Aug 2007. (Source: International Journal of Experimental Pathology)

Cardiac and aortic structural alterations due to surgically-induced menopause associated with renovascular hypertension in rats

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:24 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 301-309, Aug 2007. SummaryMenopause and hypertension independently alter cardiovascular remodelling, but little is known about their effect on left ventricular and aortic wall remodelling. Eight-weeks-old Wistar rats were divided into four groups of six animals each: Sham gr... (Source: International Journal of Experimental Pathology)

The adaptability of tendon to loading differs in men and women

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:24 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 237-240, Aug 2007. SummaryThe reason why women sustain more soft tissue injury than men during physical activity is unknown. Connective tissue properties and extracellular matrix adaptability in human tendon were investigated in models that addressed biochemical, physiologic... (Source: International Journal of Experimental Pathology)

Fell-Muir Lecture

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:23 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 201-201, Aug 2007. (Source: International Journal of Experimental Pathology)

Cell phenotypic variation in normal and damaged tendons

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:21 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 227-235, Aug 2007. SummaryInjuries to tendons are common in both human athletes as well as in animals, such as the horse, which are used for competitive purposes. Furthermore, such injuries are also increasing in prevalence in the ageing, sedentary population. Tendon disease... (Source: International Journal of Experimental Pathology)

The mechanobiological aetiopathogenesis of tendinopathy: is it the over-stimulation or the under-stimulation of tendon cells?

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:21 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 217-226, Aug 2007. SummaryWhile there is a significant amount of information available on the clinical presentation(s) and pathological changes associated with tendinopathy, the precise aetiopathogenesis of this condition remains a topic of debate. Classically, the aetiology... (Source: International Journal of Experimental Pathology)

Tendon matrix composition and turnover in relation to functional requirements

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:20 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 241-248, Aug 2007. SummaryTendons are dense regular connective tissue structures that are defined based on their anatomical position of connecting muscle to bone. Despite these obvious commons features tendons from different locations within the body show remarkable variatio... (Source: International Journal of Experimental Pathology)

Symposium: Tendon and ligament remodelling and regeneration. Foreword by Dr Graham Riley

International Journal of Experimental Pathology — Fri, 10 Aug 2007 15:23:20 +0100

International Journal of Experimental Pathology Volume 88, Issue 4, Page 215-216, Aug 2007. (Source: International Journal of Experimental Pathology)

The mouse mammary carcinoma 4T1: characterization of the cellular landscape of primary tumours and metastatic tumour foci

International Journal of Experimental Pathology — Sat, 21 Jul 2007 18:06:00 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryThe murine mammary carcinoma 4T1 causes a leukemoid reaction with profound granulocytosis coincident with the production of tumour-derived growth factors. Here, we study the evolving cellular landscape of primary tumours and metastatic tumour foci a... (Source: International Journal of Experimental Pathology)

Lysosomal acid lipase over-expression disrupts lamellar body genesis and alveolar structure in the lung

International Journal of Experimental Pathology — Sat, 21 Jul 2007 03:55:03 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryThe functional role of neutral lipids in the lung is poorly understood. Lysosomal acid lipase (LAL) is a critical enzyme in hydrolysis of cholesteryl esters and triglycerides to generate free fatty acids and cholesterol in lysosomes. Human LAL was o... (Source: International Journal of Experimental Pathology)

Laser capture microdissection reveals dose–response of gene expression in situ consequent to asbestos exposure

International Journal of Experimental Pathology — Sat, 21 Jul 2007 03:55:03 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryThe genes that mediate fibroproliferative lung disease remain to be defined. Prior studies from our laboratory showed by in situ hybridization and immunohistochemistry that the genes coding for tumour necrosis factor alpha, transforming growth facto... (Source: International Journal of Experimental Pathology)

Interplay between DtxR and nitric oxide reductase activities: a functional genomics approach indicating involvement of homologous protein domains in bacterial pathogenesis

International Journal of Experimental Pathology — Sat, 21 Jul 2007 03:55:03 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryCorynebacterium diphtheriae pathogenesis depends on the production of toxin (Dtx), which in turn depends on a micromolar concentration of nitric oxide (NO)-mediated deactivation of DtxR (an iron-dependent regulator). Inside a host, the pathogen ofte... (Source: International Journal of Experimental Pathology)

Comprehensive characterization of serum clinical chemistry parameters and the identification of urinary superoxide dismutase in a carbon tetrachloride-induced model of hepatic fibrosis in the female Hanover Wistar rat

International Journal of Experimental Pathology — Sat, 21 Jul 2007 03:55:03 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. SummaryCarbon tetrachloride (CCl4) was used to induce liver fibrosis in the rat. Using this model, we have identified changes in serum and urinary clinical chemistry parameters, and characterized histopathological lesions in the liver. Two experiments were... (Source: International Journal of Experimental Pathology)

Relationship between plasma cholesterol, von Willebrand factor concentrations, extent of atherosclerosis and antibody titres to heat shock proteins-60, -65 and -70 in cholesterol-fed rabbits

International Journal of Experimental Pathology — Tue, 19 Jun 2007 18:12:40 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Low coronary driving pressure early in the course of myocardial infarction is associated with subendocardial remodelling and left ventricular dysfunction

International Journal of Experimental Pathology — Fri, 15 Jun 2007 18:08:52 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Infiltration of polymorphonuclear cells into the post-ischaemic myocardium is dependent on β2 and α4 integrins

International Journal of Experimental Pathology — Fri, 15 Jun 2007 17:50:59 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Fell–Muir award of the British Society for Matrix Biology. From collagen chemistry towards cell therapy – a personal journey

International Journal of Experimental Pathology — Fri, 25 May 2007 18:12:15 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Extrapancreatic organ impairment during acute pancreatitis induced by bile-pancreatic duct obstruction. Effect of N-acetylcysteine

International Journal of Experimental Pathology — Fri, 25 May 2007 08:45:09 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Investigation of intratumoural and peritumoural lymphatics expressed by podoplanin and LYVE-1 in the hybridoma-induced tumours

International Journal of Experimental Pathology — Fri, 04 May 2007 18:24:30 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

BALB/c mice resistant to Toxoplasma gondii infection proved to be highly susceptible when previously infected with Myocoptes musculinus fur mites

International Journal of Experimental Pathology — Mon, 30 Apr 2007 13:14:27 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Vascular endothelial growth factor (VEGF), VEGF receptors expression and microvascular density in benign and malignant thyroid diseases

International Journal of Experimental Pathology — Mon, 30 Apr 2007 13:14:27 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Development of an experimental model of brain tissue heterotopia in the lung

International Journal of Experimental Pathology — Mon, 30 Apr 2007 09:12:59 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Central nervous system capillary haemangioblastoma: the pathologist's viewpoint

International Journal of Experimental Pathology — Mon, 30 Apr 2007 09:11:27 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Pleomorphic adenoma and adenoid cystic carcinoma: in vitro study of the impact of TGFβ1 on the expression of integrins and cytoskeleton markers of cell differentiation

International Journal of Experimental Pathology — Thu, 05 Apr 2007 18:06:45 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Differential inflammatory mediator response in vitro from murine macrophages to lactobacilli and pathogenic intestinal bacteria

International Journal of Experimental Pathology — Wed, 04 Apr 2007 16:58:42 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Mixed lineage kinases (MLKs): a role in dendritic cells, inflammation and immunity?

International Journal of Experimental Pathology — Wed, 04 Apr 2007 16:17:05 +0100

International Journal of Experimental Pathology Volume 88, Issue 2, Page 111-126, Apr 2007. (Source: International Journal of Experimental Pathology)

British Society for Matrix Biology Spring 2006 and Abstracts, Queens College, Cambridge

International Journal of Experimental Pathology — Wed, 04 Apr 2007 16:16:28 +0100

International Journal of Experimental Pathology Volume 88, Issue 2, Page A1-A45, Apr 2007. (Source: International Journal of Experimental Pathology)

Erratum

International Journal of Experimental Pathology — Wed, 28 Mar 2007 18:17:22 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Hormonal and morphological study of the pituitaries in reeler mice

International Journal of Experimental Pathology — Tue, 27 Mar 2007 19:01:27 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Ischaemia and reperfusion effects on skeletal muscle tissue: morphological and histochemical studies

International Journal of Experimental Pathology — Tue, 27 Mar 2007 19:01:24 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Reduced protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) in lymph node and liver metastases of gastric cancer

International Journal of Experimental Pathology — Tue, 13 Mar 2007 18:29:35 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Effect of reactive oxygen intermediaries on the viability and infectivity of Mycobacterium lepraemurium

International Journal of Experimental Pathology — Wed, 07 Mar 2007 19:29:58 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Erratum

International Journal of Experimental Pathology — Fri, 23 Feb 2007 19:09:31 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

c-Myc partially mediates IFNγ-induced apoptosis in the primary hepatocyte

International Journal of Experimental Pathology — Tue, 13 Feb 2007 19:06:53 +0100

International Journal of Experimental Pathology Volume 0, Issue 0, Page ???-???. (Source: International Journal of Experimental Pathology)

Characterization of Minaçu virus (Reoviridae: Orbivirus) and pathological changes in experimentally infected newborn mice

International Journal of Experimental Pathology — Mon, 22 Jan 2007 10:45:28 +0100

International Journal of Experimental Pathology Volume 88, Issue 1, Page 63-73, Feb 2007. (Source: International Journal of Experimental Pathology)

Modified method of AgNOR staining for tissue and interpretation in histopathology

International Journal of Experimental Pathology — Mon, 22 Jan 2007 10:44:09 +0100

International Journal of Experimental Pathology Volume 88, Issue 1, Page 47-53, Feb 2007. (Source: International Journal of Experimental Pathology)

Experimental cutaneous Bacillus anthracis infections in hairless HRS/J mice

International Journal of Experimental Pathology — Mon, 22 Jan 2007 10:43:42 +0100

International Journal of Experimental Pathology Volume 88, Issue 1, Page 75-84, Feb 2007. (Source: International Journal of Experimental Pathology)

Intake of melatonin is associated with amelioration of physiological changes, both metabolic and morphological pathologies associated with obesity: an animal model

International Journal of Experimental Pathology — Wed, 20 Dec 2006 19:11:18 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

Histological and serological evidence of experimental paracoccidioidomycosis in Calomys callosus (Rodentia: Cricetidae)

International Journal of Experimental Pathology — Wed, 20 Dec 2006 18:36:22 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

Motor impairment and neuronal damage following hypothermia in tropical amphibians

International Journal of Experimental Pathology — Wed, 20 Dec 2006 18:35:50 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

Histopathological study of time course changes in inter-renal aortic banding-induced left ventricular hypertrophy of mice

International Journal of Experimental Pathology — Wed, 20 Dec 2006 18:35:48 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

Cellular morphological parameters of the human urinary bladder (malignant and normal)

International Journal of Experimental Pathology — Wed, 20 Dec 2006 18:35:46 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

Inflammation-associated remodelling and fibrosis in the lung – a process and an end point

International Journal of Experimental Pathology — Wed, 20 Dec 2006 18:35:39 +0100

Online early issue. (Source: International Journal of Experimental Pathology)

DNA study of bladder papillary tumours chemically induced by N-butyl-N-(4-hydroxybutyl) nitrosamine in Fisher rats

International Journal of Experimental Pathology — Wed, 20 Dec 2006 13:28:29 +0100

Online early issue. (Source: International Journal of Experimental Pathology)