MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Food Microbiology' source. Sun, 21 Mar 2010 17:22:45 +0100 http://www.medworm.com/index.php?rid=3374238&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231042%26dopt%3DAbstract This study reports the effect of reduced a(w) and reduced Psi(m) on the germination of xerophilic fungi (represented by Eurotium herbariorum) and non-xerophilic fungi (represented by Aspergillus niger) on model glycerol agar media. Germination curves were plotted with the percentage of germinated spores against time. The germination time (t(G)), which is defined as the time at which 50% of the total viable spores have germinated, was estimated using the Gompertz model. Total viable spores was defined as those spores that were able to germinate under the optimum a(w) and Psi(m) conditions for each species, i.e. 0.95 a(w) and 2.5% agar for E. herbariorum and 0.98 a(w) and 2.5% agar for A. niger. As a(w) decreased from 0.90 to 0.85 a(w), t(G) increased significantly for both the xerophilic fu... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3374238 Tue, 02 Mar 2010 00:00:00 +0100 3374238 http://www.medworm.com/index.php?rid=3374241&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20226553%26dopt%3DAbstract Authors: Yang EJ, Chang HC The aim of this study was to purify and to identify an antifungal compound of Lactobacillus plantarum AF1, which was isolated from kimchi, and to determine if Lb. plantarum AF1 can prevent fungal growth in a particular food model system. The antifungal compound was purified using SPE and recycling prep-HPLC and its structure was elucidated using NMR and ESI-MS. The active compound from Lb. plantarum AF1 is C(12)H(22)N(2)O(2), 3,6-bis(2-methylpropyl)-2,5-piperazinedion has a molecular mass of 226. This is a new antifungal compound produced by lactic acid bacteria (LAB). To investigate the potential application of the antifungal compound to eliminate fungal spoilage in food and feed, soybean was used as a model. White mycelia and dark green spores of Aspergillu... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3374241 Wed, 24 Feb 2010 00:00:00 +0100 3374241 http://www.medworm.com/index.php?rid=3374239&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20226556%26dopt%3DAbstract This study reports the dynamics of microbial populations adhering on the surface of debittered green olives cv. Bella di Cerignola in fermentation sets inoculated with the probiotic strain Lactobacillus paracasei IMPC2.1 in different brining conditions (4% and 8% (w/v) NaCl) at room temperature and 4 degrees C. The probiotic strain successfully colonized the olive surface dominating the natural LAB population and decreasing the pH of brines to </=5.0 after 30days until the end of fermentation. The dynamics of microbial populations associated with olive surface and belonging to the different groups indicated that inoculated olives held at room temperature did not host Enterobacteriaceae at the end of fermentation. Yeast populations were present in a low number (</=log(10) 5.7CFU/g) th... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3374239 Wed, 24 Feb 2010 00:00:00 +0100 3374239 http://www.medworm.com/index.php?rid=3374240&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20226555%26dopt%3DAbstract Authors: Even S, Leroy S, Charlier C, Zakour NB, Chacornac JP, Lebert I, Jamet E, Desmonts MH, Coton E, Pochet S, Donnio PY, Gautier M, Talon R, Le Loir Y Some coagulase negative staphylococci (CNS) species play an important role in the fermentation of meat and milk products and are considered as food-grade. However, the increasing clinical significance of CNS and the presence of undesirable and unsafe properties in CNS question their presence or use in food. Our goal was to assess the safety of CNS by developing a diagnostic microarray targeting 268 genes corresponding to safety hazards in a food context i.e. toxins (especially enterotoxins) and determinants of antibiotic resistance and biogenic amine production. Target genes were selected among staphylococci and Gram-positive species... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3374240 Tue, 23 Feb 2010 00:00:00 +0100 3374240 http://www.medworm.com/index.php?rid=3362773&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20223544%26dopt%3DAbstract Authors: Gurtler JB, Rivera RB, Zhang HQ, Geveke DJ Pulsed electric field (PEF) technology has been used for the inactivation of microorganisms and to prevent flavor loss in liquid foods and beverages in place of thermal pasteurization. When used to pasteurize orange juice, PEF may prevent loss of volatile sensory attributes. Enterohemorrhagic E. coli O157:H7 (EHEC), two strains of Salmonella Typhimurium, and twenty strains of non-pathogenic bacteria were screened for inactivation in orange juice by PEF at 22 and 20kV/cm at 45 and 55 degrees C, respectively. Higher populations of both salmonellae were inactivated (2.81 and 3.54 log CFU/ml) at 55 degrees C, in comparison with the reduction of EHEC (2.22 log). When tested under the same conditions, inactivation of EHEC was slightly great... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3362773 Tue, 23 Feb 2010 00:00:00 +0100 3362773 http://www.medworm.com/index.php?rid=3362772&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20223546%26dopt%3DAbstract Authors: Ganan M, Campos G, Muñoz R, Carrascosa AV, de Pascual-Teresa S, Martinez-Rodriguez AJ The effect of growth phase on the adherence to and invasion of Caco-2 epithelial cells by five strains of Campylobacter was studied. No significant differences were observed between the behaviors in the exponential or stationary phases for the most stationary-phase tolerant strains (C. jejuni 118 and C. coli LP2), while the strains that produced a greater reduction in the viability in the stationary phase (C. jejuni 11351, C. jejuni 11168 and C. jejuni LP1), also presented reduced adherence to and invasion of Caco-2 cells. In order to find a possible explanation for the observed differences, the presence of putative virulence factors was studied in the analyzed strains. In spite of the f... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3362772 Tue, 23 Feb 2010 00:00:00 +0100 3362772 http://www.medworm.com/index.php?rid=3362775&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20219260%26dopt%3DAbstract Authors: Quirós M, Gonzalez-Ramos D, Tabera L, Gonzalez R Yeast mannoproteins are highly glycosylated proteins that are covalently bound to the beta-1,3-glucan present in the yeast cell wall. Among their outstanding enological properties, yeast mannoproteins contribute to several aspects of wine quality by protecting against protein haze, reducing astringency, retaining aroma compounds and stimulating growth of lactic-acid bacteria. The development of a non-recombinant method to obtain enological yeast strains overproducing mannoproteins would therefore be very useful. Our previous experience on the genetic determinants of the release of these molecules by Saccharomyces cerevisiae has allowed us to propose a new methodology to isolate and characterize wine yeast that overproduce m... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3362775 Fri, 19 Feb 2010 00:00:00 +0100 3362775 http://www.medworm.com/index.php?rid=3362774&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20223543%26dopt%3DAbstract Authors: Morales-Rayas R, Wolffs PF, Griffiths MW Two sample preparation methods based on electrostatic binding were tested to simultaneously separate different viral particles from different food surfaces (lettuce, strawberry, raspberries and green onions). Both methods were evaluated using a multiplex real-time PCR assay designed for detection of hepatitis A virus and norovirus GI and GII. Single and multiplex detection limits were determined as 10(1) viral particles for HAV and norovirus GII, and 10(2) viral particles for norovirus GI using artificial templates, one HAV strain and different norovirus isolates. Manual extraction based on silica columns was found more suitable for viral RNA preparation than an automatic extraction technique. Consistent detection of infectious amounts ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3362774 Fri, 19 Feb 2010 00:00:00 +0100 3362774 http://www.medworm.com/index.php?rid=3347343&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20207437%26dopt%3DAbstract Authors: Menz G, Vriesekoop F, Zarei M, Zhu B, Aldred P The aim of this study was to investigate the factors affecting the survival and growth of four food-borne pathogens (Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, and Staphylococcus aureus) in sweet and fermenting brewery wort. The Gram-negative pathogens (E. coli and Salm. Typhimurium) were capable of growth during the initial stages of fermentation in hopped wort, although they were quickly inactivated when added during the later stages of fermentation. When the wort was left unpitched, the two Gram-negative pathogens grew unabated. Pathogen growth and survival was enhanced as the pH was increased, and as both the ethanol and original gravity were decreased. Although having no effect on the Gram-negat... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3347343 Fri, 19 Feb 2010 00:00:00 +0100 3347343 http://www.medworm.com/index.php?rid=3347351&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20206394%26dopt%3DAbstract The objective of the present study was to determine the viral load of HEV in liver, loin, bladder, hepatic lymph node, bile, tonsil, plasma and faeces samples of 43 pigs at slaughter. Feline calicivirus (FCV) was used as sample process control to validate the RNA extraction process, as a confirmation of the absence of sample inhibitors and as an amplification control. Using FCV/HEV multiplex TaqMan RT-qPCR system, HEV RNA was detected in 14 out of the 43 animals tested. HEV was detected in lymph nodes (11/43), bladder (10/43), liver (9/43), bile (8/43), faeces (6/43), tonsils (3/43), plasma (1/43) samples from infected animals. No HEV-positive loin samples were observed. Viral loads of 10(3) to 10(7) copies/g were estimated in positive liver and bile samples. PMID: 20206394 [PubMed - a... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3347351 Thu, 18 Feb 2010 00:00:00 +0100 3347351 http://www.medworm.com/index.php?rid=3347350&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20206395%26dopt%3DAbstract In this study culture filtrates of the basidiomycete Lentinula edodes (LF23) were used to enhance the biocontrol activity of LS28. In vitro L. edodes culture filtrates improved the growth of C. laurentii and the activity of its catalase, superoxide dismutase and glutathione peroxidase, which play a key role in oxidant scavenging. In addition, LF23 also delayed P. expansum conidia germination. The biocontrol effect of LS28 used together with LF23 in wounded apples improved the inhibition of P. expansum growth and patulin production in comparison with LS28 alone, under both experimental and semi-commercial conditions. The biocontrol effect was confirmed by a semi-quantitative PCR analysis set up for monitoring the growth of P. expansum. PMID: 20206395 [PubMed - as supplied by publisher] ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3347350 Sun, 14 Feb 2010 00:00:00 +0100 3347350 http://www.medworm.com/index.php?rid=3347349&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20207436%26dopt%3DAbstract Authors: Porto-Fett AC, Call JE, Shoyer BE, Hill DE, Pshebniski C, Cocoma GJ, Luchansky JB We evaluated the effectiveness of fermentation, drying, and high pressure processing (HPP) to inactivate Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., and Trichinella spiralis in Genoa salami produced with trichinae-infected pork. In addition, we evaluated the effectiveness of using HPP to inactivate T. spiralis larvae in pig masseter tissue. In part A, Genoa salami batter (about 2.3log larvae/g) prepared with trichinae-infected pork was separately spiked with a five-strain cocktail of each microbial pathogen (about 7.0log CFU/g) and subsequently fermented at 20 degrees C and about 90 to 95% RH for 6h and then at 27 degrees C and about 90 to 95% RH for 26h before being dried ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3347349 Sat, 13 Feb 2010 00:00:00 +0100 3347349 http://www.medworm.com/index.php?rid=3336260&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20199820%26dopt%3DAbstract Authors: de Freitas CG, Santana AP, da Silva PH, Gonçalves VS, Barros MD, Torres FA, Murata LS, Perecmanis S The occurrence of foodborne diseases is increasing throughout the world. Bacteria of the genus Salmonella are responsible for food poisoning and, in some cases, may be fatal. The aim of this study was to adapt the multiplex PCR technique (mPCR) on the rapid and direct identification of the presence of Salmonella sp. as well as serotypes Enteritidis, Typhi and Typhimurium in poultry carcasses (n=127) and viscera (n=73). The implementation of the standard technique using positive controls was successfully adapted. The results of Salmonella sp. detection in refrigerated viscera showed that the mPCR was able to detect Salmonella genus in 2.74% of these samples. Traditional micr... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3336260 Sat, 13 Feb 2010 00:00:00 +0100 3336260 http://www.medworm.com/index.php?rid=3329155&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20193970%26dopt%3DAbstract This study underlines the necessity to use appropriate analytical tools, such as molecular methods, to fully understand the spread and persistence of L. monocytogenes in food producing companies. PMID: 20193970 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3329155 Thu, 11 Feb 2010 00:00:00 +0100 3329155 http://www.medworm.com/index.php?rid=3329156&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20188428%26dopt%3DAbstract This study produced a phenotypic characterization data set for a collection of S. bayanus var. uvarum strains, thus paving the way for industrial developments using this species as a new genetic resource. PMID: 20188428 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3329156 Wed, 10 Feb 2010 00:00:00 +0100 3329156 http://www.medworm.com/index.php?rid=3316368&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185187%26dopt%3DAbstract Authors: Echevarria R, Bautista-Gallego J, Arroyo-López FN, Garrido-Fernández A The goal of this work was to apply the Quasi-chemical primary model (a system of four ordinary differential equations that derives from a hypothetical four-step chemical mechanism involving an antagonistic metabolite) in the study of the evolution of yeast and lactic acid bacteria populations during the storage of Manzanilla-Aloreña table olives subjected to different mixtures of ascorbic acid, sodium metabisulphite and NaCl. Firstly, the Quasi-chemical model was applied to microbial count data to estimate the growth-decay biological parameters. The model accurately described the evolution of both populations during storage, providing detailed information on the microbial behaviour. Secondly,... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3316368 Wed, 10 Feb 2010 00:00:00 +0100 3316368 http://www.medworm.com/index.php?rid=3312184&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20181403%26dopt%3DAbstract The objectives of this study were to combine genotypic (pulsed-field gel electrophoresis [PFGE], 16S rRNA gene sequencing, and automated ribotyping) methods with traditional phenotypic biochemical methods to characterize a collection of Cronobacter isolates from various origins. In addition, the relative growth dynamics were compared by estimating the growth rates for each isolate in non-selective broth (BHI) at 25 degrees C and 37 degrees C. According to biochemical test profiles the majority of isolates were identified as Cronobacter sakazakii, which seemed to be the most common species distributed in the environment of PIF production plants. Furthermore, the PFGE technique displayed very high discriminatory power as 61 distinct pulsotypes were revealed among the 150 Cronobacter isolates... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3312184 Wed, 10 Feb 2010 00:00:00 +0100 3312184 http://www.medworm.com/index.php?rid=3303268&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20172617%26dopt%3DAbstract Authors: Mol S, Cosansu S, Ucok Alakavuk D, Ozturan S Survival of Salmonella Enteritidis inoculated (5-6 log cfu/g) on horse mackerel fillets was studied during salting and/or drying processes at 4 degrees C for up to 70days. Sample groups were as follows: group A (salt/fish=80/100), group B (salt/fish=30/100), group C (salted as group A and dried after 15days), group D (salted as group B and dried after 15days), and group E (dried without salting). The salt content increased in all groups with a maximum level of 29.36%. Final salt contents (%) were higher (P<0.05) in salted-dried groups (C and D) than the salted groups (A and B). Total reductions in a(w) values were found as group E>group D>group C>groups A and B (P<0.05). Salmonella survived 60days in group A, 65days i... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3303268 Sat, 06 Feb 2010 00:00:00 +0100 3303268 http://www.medworm.com/index.php?rid=3303271&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20171753%26dopt%3DAbstract Authors: McMeekin TA, Hill C, Wagner M, Dahl A, Ross T The term ecophysiology suggests that a natural connection exists between microbial ecology and microbial physiology, the former being concerned with the responses of microbial populations to environmental influences, and the latter with activities within individual cells. In this contribution we choose to integrate these as far as possible and also indicate how understanding of both is benefiting from advances in molecular biology and informatics. We consider how microbial dispersal relates to microbial survival, recovery and proliferation, including the significance of random factors (stochasticity) in continuation of bacterial lineages, observing that minor environmental changes, can greatly influence the potential for food-borne... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3303271 Mon, 01 Feb 2010 00:00:00 +0100 3303271 http://www.medworm.com/index.php?rid=3291449&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20163884%26dopt%3DAbstract Authors: Chang PK, Ehrlich KC Aspergillus flavus and Aspergillus oryzae belong to Aspergillus section Flavi. They are closely related and are of significant economic importance. The former species has the ability to produce harmful aflatoxins while the latter is widely used in food fermentation and industrial enzyme production. This review summarizes the current understanding of the similarity of the A. flavus and A. oryzae genomes, the genetic diversity in A. flavus and A. oryzae populations, the causes of this diversity, and the relatedness of nonaflatoxigenic A. flavus strains to A. oryzae. PMID: 20163884 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3291449 Mon, 01 Feb 2010 00:00:00 +0100 3291449 http://www.medworm.com/index.php?rid=3278138&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20153540%26dopt%3DAbstract Authors: Lacombe A, Wu VC, Tyler S, Edwards K We investigated the antimicrobial effect of constituents of the American cranberry (Vaccinium macrocarpon); sugar plus organic acids, phenolics, and anthocyanins, against Escherichia coli O157:H7. Each fractional component was assayed over a 24-h period with 5-log initial inocula to determine the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and log CFU/ml reductions, at their native pH and neutral pH. Each fraction produced significant reductions (P<0.05) at the native pH: MICs for sugars plus organic, phenolics, and anthocyanins were 5.6/2.6 Brix/acid (citric acid equivalents) 2.70g/L (gallic acid equivalent), and 14.80mg/L (cyanidin-3-glucoside equivalent), respectively. Sugars plus organic acids at... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278138 Mon, 01 Feb 2010 00:00:00 +0100 3278138 http://www.medworm.com/index.php?rid=3278137&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20156660%26dopt%3DAbstract Authors: Kong Q, Shan S, Liu Q, Wang X, Yu F A strain of marine Bacillus megaterium isolated from the Yellow Sea of East China was evaluated for its activity in reducing postharvest decay of peanut kernels caused by Aspergillus flavus in in vitro and in vivo tests. The results showed that the concentrations of antagonist had a significant effect on biocontrol effectiveness in vivo: when the concentration of the washed bacteria cell suspension was used at 1x10(9)CFU/ml, the percentage rate of rot of peanut kernels was 31.67%+/-2.89%, which was markedly lower than that treated with water (the control) after 7days of incubation at 28 degrees C. The results also showed that unwashed cell culture of B. megaterium was as effective as the washed cell suspension, and better biocontrol was obta... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278137 Mon, 01 Feb 2010 00:00:00 +0100 3278137 http://www.medworm.com/index.php?rid=3278136&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20156661%26dopt%3DAbstract Authors: Harapas D, Premier R, Tomkins B, Franz P, Ajlouni S Minor shoot injury to glasshouse celery, Cos lettuce and chive plants significantly increased the persistence of applied Escherichia coli (P<0.05). After 1week, mean counts of about 5 log(10) CFU/g decreased to fewer than 0.5 log(10) CFU/g on the uninjured plants, compared to 4 log(10) CFU/g or more on injured plants. By the end of the 3-week long experiments, counts from the uninjured plants were 0.21 log(10) CFU/g or fewer, but 2.8, 2.3 and 5.1 log(10) CFU/g on injured Cos lettuce, celery and chive plants, respectively. A field experiment using Cos lettuce also showed that shoot injury increased E. coli persistence. Counts from the injured plants on days 1, 3, and 7 were, 4.2, 4.1 and 3.3 log(10) CFU/g, respectively, whe... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278136 Mon, 01 Feb 2010 00:00:00 +0100 3278136 http://www.medworm.com/index.php?rid=3278156&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20153068%26dopt%3DAbstract Authors: Rivas L, McDonnell MJ, Burgess CM, O'Brien M, Navarro-Villa A, Fanning S, Duffy G The antimicrobial activities of thymol and carvacrol were assessed against a selection of verocytotoxigenic Escherichia coli (VTEC) strains (n=11) and other bacterial species and spoilage bacteria (n=7) using a model broth system. The effects of pH, temperature, water activity, sodium chloride concentrations, inoculum size and the presence of competing microflora on the activities of thymol and carvacrol against E. coli O157:H7 strain 380-94 were also determined. The minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively) and numbers of surviving E. coli O157:H7 were determined following incubation. The mean numbers of VTEC surviving exposure to thymol or carvacrol at conce... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278156 Fri, 29 Jan 2010 00:00:00 +0100 3278156 http://www.medworm.com/index.php?rid=3278146&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20153069%26dopt%3DAbstract Authors: Rúgeles LC, Bai J, Martínez AJ, Vanegas MC, Gómez-Duarte OG The prevalence of diarrheagenic Escherichiacoli in childhood diarrhea and the role of contaminated food products in disease transmission in Colombia are largely unknown. The aim of this study is to identify E. coli pathotypes, including E. coli O157:H7, from 108 stool samples from children with acute diarrhea, 38 meat samples and 38 vegetable samples. Multiplex PCR and Bax Dupont systems were used for E. coli pathotype detection. Eighteen (9.8%) E. coli diarrheagenic pathotypes were detected among all clinical and food product samples tested. Four different pathotypes were identified from clinical samples, including enteroaggregative E. coli, enterotoxigenic E. coli, shiga-toxin producing E. coli, and e... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278146 Thu, 28 Jan 2010 00:00:00 +0100 3278146 http://www.medworm.com/index.php?rid=3278140&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20153071%26dopt%3DAbstract Authors: Whitehead KA, Smith LA, Verran J A range of food soils and components (complex [meat extract, fish extract, and cottage cheese extract]; oils [cholesterol, fish oil, and mixed fatty acids]; proteins [bovine serum albumin (BSA), fish peptones, and casein]; and carbohydrates [glycogen, starch, and lactose]) were deposited onto 304 2B finish stainless steel surfaces at different concentrations (10-0.001%). Scanning electron microscopy (SEM) and epifluorescence microscopy were used to visualise the cell and food soil distribution across the surface. Epifluorescence microscopy was also used to quantify the percentage of a field covered by cells or soil. At 10% concentration, most soils, with the exception of BSA and fish peptone were easily visualised using SEM, presenting differen... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278140 Thu, 28 Jan 2010 00:00:00 +0100 3278140 http://www.medworm.com/index.php?rid=3278139&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20153539%26dopt%3DAbstract Authors: Marcó MB, Reinheimer JA, Quiberoni A Bacteriophage infection of lactic acid bacteria (LAB) constitutes one of the major problems in the dairy industry, causing economic losses and a constant risk of low quality and/or unsafe foods. The first step in the phage biology is the adsorption on the host cell surface. In a previous study, a remarkable thermal, chemical and photocatalytic resistance was demonstrated by four phages of Lactobacillus plantarum (ATCC 8014-B1, ATCC 8014-B2, FAGK1 and FAGK2). In the present work, these phages were used to characterize the adsorption process on L.plantarum ATCC 8014. Clearly, the characterization of this process could increase the possibilities of design useful strategies in order to prevent phage infections. The influence of Ca(2+), tem... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3278139 Thu, 28 Jan 2010 00:00:00 +0100 3278139 http://www.medworm.com/index.php?rid=3231589&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20116876%26dopt%3DAbstract This study investigates the preservation of orange juice using PEF in combination with nisin (2.5ppm), natamycin (10ppm), benzoic acid (BA; 100ppm), or lactic acid, (LA; 500ppm). Pichia fermentans, a spoilage yeast frequently isolated from orange juice, Escherichia coli k12 or Listeria innocua were inoculated into sterile orange juice (OJ) with, and without, added preservatives. The antimicrobial activity over time was evaluated relative to an untreated control. The effect of PEF treatment (40kV/cm, 100mus; max temperature 56 degrees C) was assessed on its own, and in combination with each antimicrobial. The acidic environment of OJ inactivated E. coli k12 (1.5log reduction) and L. innocua (0.7log reduction) slightly but had no effect on P. fermentans. PEF caused a significant decrease (P&... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3231589 Thu, 28 Jan 2010 00:00:00 +0100 3231589 http://www.medworm.com/index.php?rid=3231594&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20116124%26dopt%3DAbstract This study provides the first step towards the exploitation of the yeast wealth in China's vine-growing regions. The aim of this study was to investigate the yeast population density and diversity on three grape varieties cultivated in four representative vine-growing regions of China. Yeast species diversity was evaluated by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis of the 5.8S internal transcribed spacer (ITS) ribosomal DNA (rDNA) region of cultivable yeasts. The grapes harbored yeast populations at 10(2)-10(6)CFU/mL, consisting mostly of non-Saccharomyces species. Seventeen different yeast species belonging to eight genera were detected on the grape samples tested, including Hanseniaspora uvarum, Cryptococcus flavescens, Pi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3231594 Mon, 18 Jan 2010 00:00:00 +0100 3231594 http://www.medworm.com/index.php?rid=3231591&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20116875%26dopt%3DAbstract In this study, we investigated the relationship between cell injury and inactivation of Escherichia coli K-12 in apple juice treated with a combination of RFEF and UV-light. Apple juice purchased from a wholesale distributor was inoculated with E.coli K-12 at 7.8 log CFU/ml, processed with a laboratory scale RFEF unit at 20kHz, 15kV/cm for 170micros at a flow rate of 540ml/min followed by UV-light treatment (254nm) for 12s at 25, 30 and 40 degrees C. Treated samples were analyzed for leakage of UV-substances as a function of membrane damage and were plated (0.1ml) on Sorbitol MacConkey Agar (SMAC) and Trypticase Soy Agar (TSA) plates to determine the viability loss and percent injury. At 40 degrees C, UV-light treatment alone caused 5.8 log reduction of E. coli in apple juice while RFEF ca... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3231591 Thu, 14 Jan 2010 00:00:00 +0100 3231591 http://www.medworm.com/index.php?rid=3231587&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20116877%26dopt%3DAbstract Authors: Mataragas M, Zwietering MH, Skandamis PN, Drosinos EH The presence of Listeria monocytogenes in a sliced cooked, cured ham-like meat product was quantitatively assessed. Sliced cooked, cured meat products are considered as high risk products. These ready-to-eat, RTE, products (no special preparation, e.g. thermal treatment, before eating is required), support growth of pathogens (high initial pH=6.2-6.4 and water activity=0.98-0.99) and has a relatively long period of storage at chilled temperatures with a shelf life equal to 60days based on manufacturer's instructions. Therefore, in case of post-process contamination, even with low number of cells, the microorganism is able to reach unacceptable levels at the time of consumption. The aim of this study was to conduct a Quantit... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3231587 Thu, 14 Jan 2010 00:00:00 +0100 3231587 http://www.medworm.com/index.php?rid=3231583&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20117853%26dopt%3DAbstract Authors: Vegas C, Mateo E, González A, Jara C, Guillamón JM, Poblet M, Torija MA, Mas A The population dynamics of acetic acid bacteria in traditional vinegar production was determined in two independent vinegar plants at both the species and strain level. The effect of barrels made of four different woods upon the population dynamics was also determined. Acetic acid bacteria were isolated on solid media and the species were identified by RFLP-PCR of 16S rRNA genes and confirmed by 16S rRNA gene sequencing, while strains were typed by ERIC-PCR and (GTG)(5)-rep-PCR. The most widely isolated species was Acetobacter pasteurianus, which accounted for 100% of all the isolates during most of the acetification. Gluconacetobacter europaeus only appeared at any notable level at the en... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3231583 Thu, 14 Jan 2010 00:00:00 +0100 3231583 http://www.medworm.com/index.php?rid=3213172&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20096471%26dopt%3DAbstract In this study, we revealed that Japanese industrial baker's yeast possesses one MPR gene. The nucleotide sequence of the MPR gene in industrial baker's yeast was identical to the MPR2 gene in Sigma1278b strain. Gene disruption analysis showed that the MPR2 gene in industrial baker's yeast is involved in air-drying stress tolerance by reducing the intracellular oxidation levels. We also found that expression of the Lys63Arg and Phe65Leu variants with enhanced enzymatic activity and stability, respectively, increased the fermentation ability of bread dough after exposure to air-drying stress compared with the wild-type Mpr1. In addition, our recent study showed that industrial baker's yeast cells accumulating proline exhibited enhanced freeze tolerance in bread dough. Proline accumulation al... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3213172 Mon, 11 Jan 2010 00:00:00 +0100 3213172 http://www.medworm.com/index.php?rid=3213171&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20096472%26dopt%3DAbstract Authors: Masud U, Matsushita K, Theeragool G The adhS gene which encodes the smallest subunit, subunit III, of quinoprotein alcohol dehydrogenase (PQQ-ADH) from Acetobacter pasteurianus SKU1108 has been cloned and characterized. The role of this subunit on the function of PQQ-ADH was investigated by construction of adhS gene disruptant and mutants. The adhS gene disruptant completely lost its PQQ-ADH activity and acetate-producing ability but retained acetic acid toleration. In contrast, this disruptant grew well, even better than the wild type, in the ethanol containing medium even though its PQQ-ADH activity and ethanol oxidizing ability was completely lost, while NAD(+)-dependent ADH (NAD(+)-ADH) was induced. Heme staining and immunoblot analysis of both membrane and soluble fractio... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3213171 Mon, 11 Jan 2010 00:00:00 +0100 3213171 http://www.medworm.com/index.php?rid=3213170&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20100628%26dopt%3DAbstract In this study survival of STEC and non-STEC on surfaces under various humidities, temperatures and in the presence of different types of soil was investigated. A model system with controlled relative humidity and temperature was established by using saturated salt solutions. All the 12 STEC strains had a reduction in viable count during incubation at 70% RH at 12 degrees C. The reduction was 2-3.5 log and 4.5-5.5 log after 1 and 7days of incubation, respectively. Surviving cells were observed after 19days of incubation. The STEC strains were more resistant to desiccation than non-STEC strains. STEC survived better at 12 degrees C, compared to 20 degrees C. The survival of STEC was much lower than the survival of a Staphylococcus simulans strain tested, which showed less than 1 log reductio... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3213170 Mon, 11 Jan 2010 00:00:00 +0100 3213170 http://www.medworm.com/index.php?rid=3194707&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20089325%26dopt%3DAbstract Authors: Furukawa S, Akiyoshi Y, O'Toole GA, Ogihara H, Morinaga Y Effects of food additives on biofilm formation by food-borne pathogenic bacteria were investigated. Thirty-three potential food additives and 3 related compounds were added to the culture medium at concentrations from 0.001 to 0.1% (w/w), followed by inoculation and cultivation of five biofilm-forming bacterial strains for the evaluation of biofilm formation. Among the tested food additives, 21 showed inhibitory effects of biofilm formation by Staphylococcus aureus and Escherichia coli, and in particular, sugar fatty acid esters showed significant anti-biofilm activity. Sugar fatty acid esters with long chain fatty acid residues (C14-16) exerted their inhibitory effect at the concentration of 0.001% (w/w), but bacterial... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3194707 Thu, 07 Jan 2010 00:00:00 +0100 3194707 http://www.medworm.com/index.php?rid=3164531&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20060189%26dopt%3DAbstract Authors: Chen J, Zhang L, Paoli GC, Shi C, Tu SI, Shi X A 5'-nuclease real-time PCR assay using a minor groove binding probe was developed for the detection of Salmonella enterica from artificially contaminated foods. S.enterica-specific sequences were identified by a comparative genomic approach. Several species-specific target sequences were evaluated for specificity. A real-time PCR assay was developed targeting a nucleotide sequence within the putative type III secretion ATP synthase gene (ssaN). An internal amplification control (IAC) probe was designed by randomly shuffling the target probe sequence and a single-stranded oligonucleotide was synthesized to serve as an IAC. The assay demonstrated 100% inclusivity for the 40 Salmonella strains tested and 100% exclusivity for 24 non-... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3164531 Wed, 06 Jan 2010 00:00:00 +0100 3164531 http://www.medworm.com/index.php?rid=3164532&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20060188%26dopt%3DAbstract Authors: Govaris A, Solomakos N, Pexara A, Chatzopoulou PS The antimicrobial effect of oregano essential oil (EO) at 0.6 or 0.9%, nisin at 500 or 1000IU/g, and their combination against Salmonella Enteritidis was studied in minced sheep meat during storage at 4 degrees or 10 degrees C for 12days. Sensory evaluation showed that the addition of oregano EO at 0.6 or 0.9% in minced sheep meat was organoleptically acceptable, and attribute scores were higher for the EO at 0.6 than 0.9%. According to compositional analysis of the oregano EO, the phenols carvacrol (80.15%) and thymol (4.82%) were the predominant components. Treatment of minced sheep meat with nisin at 500 or 1000IU/g, proved insufficient to act against S. Enteritidis. The combination of the oregano EO at 0.6% with nisin at 50... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3164532 Thu, 24 Dec 2009 00:00:00 +0100 3164532 http://www.medworm.com/index.php?rid=3164530&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20060611%26dopt%3DAbstract Authors: Qin G, Zong Y, Chen Q, Hua D, Tian S Boron, an essential plant micronutrient, was effective in the form of potassium tetraborate for control of postharvest gray mold caused by Botrytis cinerea on table grapes stored at room temperature or at 0 degrees C. The inhibition of fruit decay was closely correlated with boron concentrations and partially influenced by pH value of the solution. Boron strongly inhibited spore germination, germ tube elongation, and mycelial spread of B. cinerea in the culture medium. Application of boron at 1% caused the appearance of abnormal spores (disrupted) in some cases. By using propidium iodide fluorescent staining, loss of membrane integrity in B. cinerea was observed after boron treatment. Furthermore, boron led to the leakage of cellular consti... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3164530 Thu, 24 Dec 2009 00:00:00 +0100 3164530 http://www.medworm.com/index.php?rid=3130156&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20036024%26dopt%3DAbstract Authors: Zhou G, Zheng D, Dou L, Cai Q, Yuan Z The occurrences of Bacillus cereus group strains in 40 ice cream samples were investigated. Among 109 isolated B. cereus group strains confirmed by 16S rDNA sequence analysis only 50 were identified as B. cereus and one as B. thuringiensis by using FDA (U.S. Food and Drug Administration) standard, indicating the two identification standards were highly inconsistent. Furthermore, the psychrotolerant growth properties and the occurrence of specific psychrotolerant genes of the isolates were also studied. Both psychrotolerant 16S rDNA fragments and enterotoxic genes could be detected among mesophilic and psychrotolerant strains. No relationship among psychrotolerance, presence of psychrotolerant 16S rDNA fragments and enterotoxic genes were f... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3130156 Wed, 23 Dec 2009 00:00:00 +0100 3130156 http://www.medworm.com/index.php?rid=3115482&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20022397%26dopt%3DAbstract The objective of this study was to evaluate the fate of E. coli O157:H7 and Salmonella on fresh (4 degrees C, 12 degrees C and 23 degrees C) and frozen (-20 degrees C) cut mangoes and papayas. Cut mangoes and papayas were spot inoculated with either a four-strain or five-strain cocktail of E. coli O157:H7 or Salmonella, respectively. Inoculated samples were air dried, placed in containers and stored at 4+/-2, 12+/-2, 23+/-2 and -20+/-2 degrees C. Samples were enumerated following stomaching on nonselective and selective media at days 0, 1, 3, 5, 7, 10, 14, 21 and 28 (4+/-2 and 12+/-2 degrees C); 0, 1, 3, 5 and 7 (23+/-2 degrees C); and 0, 7, 14, 21, 28, 60, 90, 120, 150 and 180 (-20+/-2 degrees C). Population levels (log CFU/g) of fruit were calculated. E. coli O157:H7 and Salmonella have ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3115482 Thu, 17 Dec 2009 00:00:00 +0100 3115482 http://www.medworm.com/index.php?rid=3105620&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015563%26dopt%3DAbstract This study evaluated the influence of the essential oil from Origanum vulgare L. on the enterotoxin production, membrane permeability and cell surface characteristics of Staphylococcus aureus. The suppression of enterotoxin production occurred totally in the broth added with the essential oil at subinhibitory concentrations (0.3 and 0.15microL/mL). Loss of 260-nm-absorbing material and potassium ions occurred immediately after addition of the essential oil at 0.6 and 1.2microL/mL and followed up to 120min. Electron microscopy of essential oil-treated cells revealed the formation of roles in the cell surfaces and loss of cytoplasm material. According to these results, O. vulgare essential oil could be rationally applied in food products both to inhibit the growth of S. aureus and to suppres... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3105620 Fri, 04 Dec 2009 00:00:00 +0100 3105620 http://www.medworm.com/index.php?rid=3105619&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20018397%26dopt%3DAbstract Authors: Neetoo H, Chen H The application of high hydrostatic pressure (HHP) at a level of 600MPa at 20 degrees C to decontaminate crimson clover, red clover, radish and broccoli seeds inoculated with E. coli O157:H7 and Salmonella were evaluated. Salmonella was generally more pressure-resistant than E. coli O157:H7 on clover and radish seeds except on broccoli seeds where the trend was reversed. In addition, the application of HHP differentially affected seeds' germinability and the order of pressure tolerance of the seeds was such that red clover>crimson clover approximately broccoli>radish seeds with final germination percentages ranging from 85-100% while their untreated counterparts had final germination percentages of 99-100%. Pre-soaking the different types of seeds in wat... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3105619 Thu, 03 Dec 2009 00:00:00 +0100 3105619 http://www.medworm.com/index.php?rid=3049277&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19948367%26dopt%3DAbstract This study evaluated the use of microencapsulation to maintain probiotic Lactobacillus rhamnosus GG (LGG) viability during exposure to detrimentally high levels of water activity. Freeze-dried LGG was mixed into a whey protein solution and sprayed onto a core particle. A second batch was prepared by applying an extra layer of palm oil onto the LGG encapsulates. Viability of the LGG preparations was measured over time in an accelerated shelf-life study at 37 degrees C which included exposure to different water activities (a(w)=0.15 or 0.7), ambient oxygen, and incorporation of the probiotic in infant formula powder. Results showed that a high water activity (0.7) was detrimental to the survival of unencapsulated LGG, yielding more than a 10log(10) loss in cell viability within 2weeks. The p... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3049277 Sat, 28 Nov 2009 00:00:00 +0100 3049277 http://www.medworm.com/index.php?rid=3040462&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19944473%26dopt%3DAbstract This study emphasizes the importance of aerobic spore-forming bacteria in raw milk as the species that are able to produce toxins and/or spoilage enzymes are all abundantly present in raw milk. Moreover, we demonstrated that some strains are capable of growing at room temperature and staying stable at refrigeration temperatures. PMID: 19944473 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3040462 Thu, 26 Nov 2009 00:00:00 +0100 3040462 http://www.medworm.com/index.php?rid=3033976&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19939484%26dopt%3DAbstract Authors: Hata T, Tanaka R, Ohmomo S Bacteriocins produced by lactic acid bacteria showing stability even in neutral and weak alkaline pH were screened, and a new bacteriocin produced by Lactobacillus plantarum A-1, plantaricin ASM1 (PASM1) was purified and characterized. This bacteriocin which is heat-stable but digested by trypsin inhibits the growth of lactic acid bacterial species, such as Lactobacillus, Leuconostoc, and Enterococcus. PASM1 showed stability in a wide pH range compared to nisin A. The bacteriocin was purified using cation exchange, hydrophobic interaction, and reverse-phase high-performance liquid chromatography. The activity of the purified bacteriocin was obtained as one fraction. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3033976 Tue, 10 Nov 2009 00:00:00 +0100 3033976 http://www.medworm.com/index.php?rid=3033978&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19932925%26dopt%3DAbstract Authors: Sørensen JL, Aveskamp MM, Thrane U, Andersen B Strains of the genus Phoma are often isolated from various environmental samples including cereals and maize. In the present study we performed a chemical characterization of strains isolated from Danish samples derived from whole plant material collected at harvest. All strains were isolated using a recently developed isolation medium and identified morphologically as P. pomorum. This species is placed in the Phoma section Peyronellaea and strains of other members in this section were also included in the present study. Sequence analysis of the internal transcribed spacer region (ITS) grouped the Danish P. pomorum strains with representative P. pomorum strains isolated from other sources. The metabolite production on dichlor... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3033978 Fri, 06 Nov 2009 00:00:00 +0100 3033978 http://www.medworm.com/index.php?rid=3033975&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19939485%26dopt%3DAbstract This study confirmed also the potentiating effect of a mild temperature treatment on the antimicrobial efficacy of the molecules. Neither the thermal treatment alone nor the presence of the terpenes at their maximum concentrations (without thermal treatment) were able to guarantee the microbial stability of the beverages. PMID: 19939485 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3033975 Fri, 06 Nov 2009 00:00:00 +0100 3033975 http://www.medworm.com/index.php?rid=3015830&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926155%26dopt%3DAbstract Authors: White AS, Godard RD, Belling C, Kasza V, Beach RL Ninety beverages of three types (sugar sodas, diet sodas and water) were obtained from 20 self-service and 10 personnel-dispensed soda fountains, analyzed for microbial contamination, and evaluated with respect to U.S. drinking water regulations. A follow-up study compared the concentration and composition of microbial populations in 27 beverages collected from 9 soda fountain machines in the morning as well as in the afternoon. Ice dispensed from these machines was also examined for microbial contamination. While none of the ice samples exceeded U.S. drinking water standards, coliform bacteria was detected in 48% of the beverages and 20% had a heterotrophic plate count greater than 500cfu/ml. Statistical analyses revealed no d... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015830 Fri, 06 Nov 2009 00:00:00 +0100 3015830 http://www.medworm.com/index.php?rid=3015829&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926156%26dopt%3DAbstract This study hypothesized that this difference did not originate from the structure of water but based on the OH radical (OH). It was proved by the UV spectroscopy, (17)O-NMR spectroscopy and electron spin resonance analysis. NEW contains more OH compared with AcEW in the same available chlorine concentration level. The OH that exists in NEW and AcEW was found to have an important fungicidal factor that destroys the cellular structures of the A. flavus conidia. It also damages the cellular normal function of A. flavus conidia that brought about K(+) and Mg(2+) leakages. The levels of OH that exist in NEW and AcEW could be the important reason that leads to significant fungicidal efficiencies against A. flavus. PMID: 19926156 [PubMed - as supplied by publisher] (Source: International Jour... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015829 Fri, 06 Nov 2009 00:00:00 +0100 3015829 http://www.medworm.com/index.php?rid=3015833&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19923029%26dopt%3DAbstract This study investigated the effectiveness of heat treatment and Pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. In vitro experiments demonstrated that heat treatment at 38 degrees C significantly inhibited mycelial growth of three different pathogens (Botrytis cinerea, Alternaria alternata and Rhizopus stolonifer Ehrenb). In vivo experiments unveiled that either heat treatment or P. guilliermondii reduced decay caused by these pathogens. Furthermore, a combination of heat treatment followed by the application of P. guilliermondii (H+P) provided the best efficacy in prevention of cherry tomato from fungal spoilage. Following, H+P treatment, electronic nose detected a reduction of volatility in cherry tomato fruit odor, an ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015833 Thu, 05 Nov 2009 00:00:00 +0100 3015833 http://www.medworm.com/index.php?rid=3015832&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19923030%26dopt%3DAbstract Authors: Somolinos M, García D, Mañas P, Condón S, Pagán R Stationary growth phase cells of Escherichiacoli were more pulsed electric fields (PEF) resistant in citrate-phosphate McIlvaine buffer at pH 4.0 than at pH 7.0. The greater PEF resistance was also confirmed in fruit juices of similar acid pH. In this work we studied whether the higher PEF resistance of E. coli at acid pH was due to the low pH itself or to the interaction of the components of the treatment medium with the cells. The protective effect on E. coli cells was due to the presence of organic acids such as citric, acetic, lactic or malic at pH 4.0. The protective effect of citric acid at pH 4.0 depended on its concentration. A linear relationship was observed between the Log(10) of the citric acid c... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015832 Sat, 31 Oct 2009 00:00:00 +0100 3015832 http://www.medworm.com/index.php?rid=3015834&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19923028%26dopt%3DAbstract Authors: Mutharia LM, Klassen MD, Fairles J, Barbut S, Gill CO Blood, liver, kidney, lymph nodes and muscle tissue were obtained from the carcasses of five cows with advanced Johne's disease. Samples from the raw tissues, from cooked muscle tissues and from cooked hamburger patties that contained chopped mesenteric lymph nodes were collected aseptically. Each sample was divided into two portions, one of which was decontaminated. Both portions were homogenized. Homogenates were spread on selective agar for the recovery of Mycobacterium avium subsp. paratuberculosis (Map) and inoculated into a Map growth medium with the organism being detected in the cultures by PCR procedures and Ziehl-Neelsen staining. Map were recovered at numbers >10(3)cfu/g from 7 of 15 liver and mesenteric and i... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015834 Fri, 30 Oct 2009 00:00:00 +0100 3015834 http://www.medworm.com/index.php?rid=3015831&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19923031%26dopt%3DAbstract Authors: Wang S, Kong J, Gao C, Guo T, Liu X A new virulent phage (phiLdb) of Lactobacillus delbrueckii subsp. bulgaricus was isolated from a Chinese yogurt sample showing slow acidification. It belonged to the Siphoviridae family with an icosahedral capsid of 47.7+/-0.9nm in diameter and a long tail of 129.8+/-2nm. The genome of phage phiLdb was estimated to be approximately 41kbp, and did not contain cohesive ends. One-step growth kinetics of its lytic development revealed latent and burst periods of 45min and 75min, respectively, with a burst size of 56+/-2 phage particles per infected cell. Phage phiLdb was highly specific for Lactobacillus delbrueckii subsp. bulgaricus. The presence of calcium or magnesium ions was necessary to accelerate cell lysis and improve plaque formation. P... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3015831 Fri, 30 Oct 2009 00:00:00 +0100 3015831 http://www.medworm.com/index.php?rid=3001408&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19913932%26dopt%3DAbstract In conclusion, data presented in this study have revealed considerable differences in the ability of L. monocytogenes strains to invade host cells and suggest the presence of additional factors that may contribute to adhesion and invasion. Virulence of L. monocytogenes is still not fully understood in some respects. Further studies focused on the mechanisms of L. monocytogenes pathogenicity together with the development of more reliable and efficient methods for virulence determination in this species are still required. PMID: 19913932 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3001408 Thu, 29 Oct 2009 00:00:00 +0100 3001408 http://www.medworm.com/index.php?rid=3001407&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19913934%26dopt%3DAbstract The objective of this work was to introduce an alternative Poisson-based distribution framework capable of representing this kind of data without incurring loss of information. The negative binomial, and two zero-modified parameterisations of the Poisson and negative binomial distributions (zero-inflated and hurdle) were fitted to actual zero-inflated bacterial data consisting of total coliforms (n=590) and Escherichia coli (n=677) present on beef carcasses sampled from nine Irish abattoirs. Improvement over the simple Poisson was shown by the simple negative binomial (p=0.426 for chi(2) test for the coliforms data) due to the added heterogeneity parameter, although it slightly overestimated the zero counts and underestimated the first few positive counts for both data sets. Whereas, the z... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3001407 Wed, 28 Oct 2009 00:00:00 +0100 3001407 http://www.medworm.com/index.php?rid=3001406&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19914726%26dopt%3DAbstract Authors: Garre E, Raginel F, Palacios A, Julien A, Matallana E The tolerance of the yeast Saccharomyces cerevisiae to desiccation is important for the use of this microorganism in the wine industry, since active dry wine yeast is routinely used as starter for must fermentations. Many studies have shown the complexity of the cellular effects caused by water loss, including oxidative injuries on macromolecular components. However the technological interest of yeast drying was not addressed in those studies, and the dehydration conditions were far from the industrial practice. In the present study a molecular approach was used to characterize the relevant injuring conditions during pilot plant dehydrations under two different drying temperatures (i.e., 35 and 41 degrees C). We have analyz... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=3001406 Wed, 28 Oct 2009 00:00:00 +0100 3001406 http://www.medworm.com/index.php?rid=2992922&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19906457%26dopt%3DAbstract Authors: Nogueira JH, Gonçalez E, Galleti SR, Facanali R, Marques MO, Felício JD Aflatoxin B(1) (AFB(1)) is a highly toxic and carcinogenic metabolite produced by Aspergillus species on food and agricultural commodities. Inhibitory effects of essential oil of Ageratum conyzoides, on the mycelial growth and aflatoxin B(1) production by Aspergillus flavus were studied. Cultures were incubated in yeast extract-sucrose (YES) broth for days at 25 degrees C at the following different concentrations of the essential oil (from 0.0 to 30mug/mL). The essential oil inhibited fungal growth to different extents depending on the concentration, and completely inhibited aflatoxin production at concentrations above 0.10microg/mL. The analysis of the oil by GC/MS showed that its main component... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2992922 Sat, 24 Oct 2009 00:00:00 +0100 2992922 http://www.medworm.com/index.php?rid=2978149&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897269%26dopt%3DAbstract The objectives of this work were to characterize the recovered isolates phenotypically and to evaluate the use of a Pulsed-Field Gel Electrophoresis (PFGE) protocol for Cronobacter spp. subtyping. Out of 23 isolates studied from three brands of PIF (20 of brand A, 1 of brand B and 2 of brand C), 22 were identified as C. sakazakii and 1 as C. malonaticus. All isolates were susceptible to twelve antimicrobial agents assayed. The 19 C. sakazakii isolates of brand A showed five XbaI-PFGE patterns and the genetic clusters revealed by XbaI were confirmed with a second restriction enzyme, SpeI. The isolate from brand B showed the same XbaI and SpeI patterns as those of a group of isolates of brand A, suggesting a possible common source of contamination. The C. sakazakii isolates of brand C exhibi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2978149 Fri, 23 Oct 2009 00:00:00 +0100 2978149 http://www.medworm.com/index.php?rid=2978148&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897270%26dopt%3DAbstract This study evaluates the effect of individual environmental parameters on 3-HPA production by Lactobacillus reuteri DSMZ 20016, which only proved possible under conditions that allow accumulation well below the threshold concentration affecting cell viability. 3-HPA production was optimal at pH 6 and in the presence of 300mM glycerol. Production increased with an increase in cell concentration up to an OD(600) of 50, whereas higher cell concentrations inhibited accumulation. Data presented in this study suggest that 3-HPA plays a role in regulating its own production through quorum sensing. Glycerol dehydratase possessing bacterial strains isolated from South African red wine, L. pentosus and L. brevis, tested positive for 3-HPA accumulation. 3-HPA is normally intracellularly reduced to 1,... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2978148 Thu, 22 Oct 2009 00:00:00 +0100 2978148 http://www.medworm.com/index.php?rid=2978150&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19896745%26dopt%3DAbstract In this study the inactivation of MAP was studied in a fluid homogenate of lamb skeletal muscle at temperatures previously identified as being relevant to cooking processes applied by domestic consumers. A PCR thermocycler was used to ensure accurate temperatures and rapid heat exchange, while radiometric culture was used to ensure sensitive detection of viable MAP for determination of D and z values. Among the two predominant strains of MAP, S and C, D(55) ranged from 56 to 89min, D(60) was 8 to 11min, D(65) was 26 to 35s while D(70) was 1.5 to 1.8s. Values for z were 4.21C degrees for the S strain and 4.51C degrees for the C strain. At temperatures of 65-70 degrees C, MAP appeared to be less heat tolerant in skeletal muscle fluid than in previous reports using milk as the medium. The tot... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2978150 Wed, 21 Oct 2009 00:00:00 +0100 2978150 http://www.medworm.com/index.php?rid=2968243&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19892425%26dopt%3DAbstract In conclusion, we suggest that anisakiasis in Japan is mainly caused by A. simplex sensu stricto because it penetrates the muscle of the fish at a higher rate than A. pegreffii. PMID: 19892425 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2968243 Tue, 20 Oct 2009 00:00:00 +0100 2968243 http://www.medworm.com/index.php?rid=2968242&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19892426%26dopt%3DAbstract Authors: Menniti AM, Gregori R, Neri F The ability of trans-2-hexenal, carvacrol and eugenol to control F. verticillioides was explored in vitro and in artificially infected kernels. The effect of the trans-2-hexenal fumigation on F. verticillioides control, fumonisin production and kernel germination was also investigated in naturally infected kernels. Trans-2-hexenal, carvacrol and eugenol vapour showed fungicidal activity against F. verticillioides, in in vitro trials. Trans-2-hexenal was the best pathogen inhibitor, followed by carvacrol and eugenol. In maize kernels, fumigations with trans-2-hexenal provided a high inhibitory effect on F. verticillioides growth and its efficacy depended on concentration and time of incubation. The most effective dose of trans-2-hexenal was 369muL/... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2968242 Sat, 17 Oct 2009 00:00:00 +0100 2968242 http://www.medworm.com/index.php?rid=2954991&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19879008%26dopt%3DAbstract Authors: Fadda ME, Viale S, Deplano M, Pisano MB, Cosentino S The occurrence of yeast microflora in raw goat's milk collected from 62 dairy farms located in different areas of Sardinia was evaluated. Candida zeylanoides was the most frequently occurring species followed by different Basidiomycetous species. In the strains isolated some biochemical characteristics of technological interest were investigated and a predominance of lipolytic yeast species was found. We employed a simple method of DNA extraction that in a minimal time and with low-cost provided a high quality of DNA for RAPD analysis of 32 isolates of C. zeylanoides. The primers M13 and CDU were used and at 40% of similarity, two distinct clusters were observed. The presence of C. krissii species was supposed but further mo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2954991 Sat, 17 Oct 2009 00:00:00 +0100 2954991 http://www.medworm.com/index.php?rid=2948556&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19875188%26dopt%3DAbstract Authors: Rasooly R, Do PM Shiga toxin-producing Escherichia coli have been associated with food-borne illnesses. Pasteurization is used to inhibit microbial growth in milk, and an open question is whether milk pasteurization inactivates Shiga toxins. To answer this question we measured Shiga toxin's inhibition effect on Vero cell dehydrogenase activity and protein synthesis. Our data demonstrate that Shiga toxin 2 (Stx2) is heat-stable and that pasteurization of milk, at the various suggested temperatures and times by the U.S. Food and Drug Administration, (63 degrees C for 30min, or 72 degrees C for 15s or 89 degrees C for 1s), did not reduce the biological activity of Stx2. However, treatment at 100 degrees C for 5min inactivated the toxin. These data demonstrate that Stx2 is not ina... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2948556 Tue, 13 Oct 2009 00:00:00 +0100 2948556 http://www.medworm.com/index.php?rid=2944437&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19861230%26dopt%3DAbstract The objective of this study was to develop an effective antimicrobial edible coating containing organic salts to control the growth of L. monocytogenes in CSS slices and fillets. An in-house made formulation consisting of sodium lactate (SL, 0-2.4%) and sodium diacetate (SD, 0-0.25%) as well as 2.5% OptiForm (a commercial formulation of SL and SD) were incorporated into five edible coatings: alginate, kappa-carrageenan, pectin, gelatin or starch. The coatings were applied onto the surface of CSS slices inoculated with L. monocytogenes to an inoculum level of 500CFU/cm(2) ( approximately 3 log CFU/g) and stored at room temperature (22 degrees C) for 6days. Alginate coating was found to be the most effective carrier for the various antimicrobial treatments in inhibiting the growth of L. mono... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2944437 Tue, 13 Oct 2009 00:00:00 +0100 2944437 http://www.medworm.com/index.php?rid=2944436&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19864033%26dopt%3DAbstract Authors: Liao H, Zhang L, Hu X, Liao X Apple juice was pasteurized by high pressure carbon dioxide (HPCD) at 20MPa with CO(2) concentration of 4.5-5.3% and mild heat (MH) at atmospheric pressure. Microbial inactivation and stability of natural microorganisms in apple juice were investigated. The temperatures were 37, 42, 47, 52, 57 and 62 degrees C, treatment time was 30min, and storage temperatures of pasteurized apple juice were 2 and 28 degrees C. The aerobic bacteria (AB) treated by MH at 62 degrees C and by HPCD at>/=52 degrees C were almost totally inactivated, the microbial counts were<10CFU/mL. The yeasts and moulds (Y&M) treated by MH at >/=57 degrees C and by HPCD at >/=42 degrees C were totally inactivated. HPCD increased the susceptibility of these natural m... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2944436 Tue, 13 Oct 2009 00:00:00 +0100 2944436 http://www.medworm.com/index.php?rid=2935659&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19853944%26dopt%3DAbstract Authors: Cerf O, Carpentier B, Sanders P There are concerns that more extensive application of disinfectants in the food industry could result in increased resistance of bacteria to antibiotics and that therapeutic failure could ensue. This paper highlights the differences in application and mode of action between antibiotics in human or animal medicine and disinfectants in the food industry. It describes the completely different methods used to determine in-use concentrations in the two contexts. It points out that the minimum inhibitory concentration (MIC) is never the concentration at which disinfectants should be applied. It also discusses erroneous conclusions that may be drawn when the failure of therapy or disinfection is attributed to intrinsic properties of the molecules rathe... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2935659 Thu, 08 Oct 2009 00:00:00 +0100 2935659 http://www.medworm.com/index.php?rid=2935658&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19853945%26dopt%3DAbstract In conclusion, our study has provided insights in phenotype-associated, and general and acidulant-specific responses in B. cereus. PMID: 19853945 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2935658 Mon, 05 Oct 2009 00:00:00 +0100 2935658 http://www.medworm.com/index.php?rid=2920462&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846233%26dopt%3DAbstract Authors: Stratford M, Plumridge A, Nebe-von-Caron G, Archer DB Fungal spoilage of many foods is prevented by weak-acid preservatives such as sorbic acid or acetic acid. We show that sorbic and acetic acids do not both inhibit cells by lowering of internal pH alone and that the "classical weak-acid theory" must be revised. The "classical weak-acid theory" suggests that all lipophilic acids with identical pK(a) values are equally effective as preservatives, causing inhibition by diffusion of molecular acids into the cell, dissociation, and subsequent acidification of the cytoplasm. Using a number of spoilage fungi from different genera, we have shown that sorbic acid was far more toxic than acetic acid, and no correlation existed between resistance to acetic acid and resistance to sorbic... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2920462 Sat, 03 Oct 2009 23:00:00 +0100 2920462 http://www.medworm.com/index.php?rid=2902178&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19833401%26dopt%3DAbstract Authors: González M, Skandamis PN, Hänninen ML Campylobacter is one of the leading causes of foodborne bacterial enteritis. Since chicken meat may be an important source of C. jejuni, the aims of this study were (i) to evaluate the survival/inactivation of C. jejuni strain 49/7R and its antimicrobial resistant variants (49/7RAT and 49/7RATCIP32) in minced chicken meat during extended storage at temperatures ranging from -20 degrees C to 25 degrees C and (ii) to test the suitability of the Weibull model for predicting the inactivation of C. jejuni in minced chicken meat in a wide range of temperatures. Minced chicken meat samples were inoculated with C. jejuni and log CFU/g were counted after different storage times at -20 degrees C, -5 degrees C, 4 degrees C, 15 degrees C or ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2902178 Tue, 29 Sep 2009 23:00:00 +0100 2902178 http://www.medworm.com/index.php?rid=2887571&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19822375%26dopt%3DAbstract Diversity and evolution of the microbial populations during manufacture and ripening of Casín, a traditional Spanish, starter-free cheese made from cow's milk. Int J Food Microbiol. 2009 Sep 30; Authors: Alegría A, Alvarez-Martín P, Sacristán N, Fernández E, Delgado S, Mayo B Classical culturing and denaturing gradient gel electrophoresis (DGGE) techniques have been used for studying the microbial diversity and dynamics of the traditional Spanish Casín cheese during manufacturing and ripening. As with other starter-free cheeses made from raw milk, the microbial diversity of Casín was shown to be high by both culturing and DGGE analyses. The culture technique showed that lactic acid bacteria (LAB) species constituted the majority of the microbi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2887571 Tue, 29 Sep 2009 23:00:00 +0100 2887571 http://www.medworm.com/index.php?rid=2887572&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19822374%26dopt%3DAbstract Authors: Powell MR Domestic and international food safety policy developments have spurred interest in the design and interpretation of experimental growth challenge studies to determine whether ready-to-eat (RTE) foods are able to support growth of Listeria monocytogenes. Existing challenge study protocols and those under development differ markedly in terms of experimental design and the acceptance criteria under which a RTE food is determined not to support L. monocytogenes growth. Consequently, the protocols differ substantially with respect to the probability of incorrectly determining that growth occurs and the statistical power to detect growth if it does occur. Applying a fixed acceptance criteria exceedance value (e.g., less than a 0.5 log(10) or 1 log(10) increase) to disting... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2887572 Sat, 26 Sep 2009 23:00:00 +0100 2887572 http://www.medworm.com/index.php?rid=2887574&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19819038%26dopt%3DAbstract Authors: Bae YY, Lee HJ, Kim SA, Rhee MS We investigated the lethal effect of supercritical carbon dioxide (SC-CO(2); temperature: 65, 70 degrees C, pressure: 80, 100, 120bar, time: 10-40min) on Alicyclobacillus acidoterrestris spores (10(6)-10(7)spores/ml) suspended in apple juice. A. acidoterrestris spores were completely inactivated by SC-CO(2) to undetectable levels in above 65 degrees C, 100bar for 40min and 70 degrees C, 80bar for 30min. The SC-CO(2) did not affect (p>0.05) the pH and Brix of apple juice. In electron microscopic observations, the surface and internal morphological changes and extraction of intracellular materials of the treated spores were observed. Our results indicate that SC-CO(2) can effectively kill A. acidoterrestris spores in apple juice with no changes... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2887574 Fri, 25 Sep 2009 23:00:00 +0100 2887574 http://www.medworm.com/index.php?rid=2887573&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19819579%26dopt%3DAbstract Authors: Gil-Serna J, Vázquez C, Sardiñas N, González-Jaén MT, Patiño B Ochratoxin A (OTA) is one of the most important mycotoxins because of its high toxicity to both humans and animals and its occurrence in a number of basic foods and agro-products. Some of the main OTA-producing species belong to Aspergillus section Circumdati, whose taxonomy has been lately revised with the description of new species. The high morphological similarity of these species (Aspergillusochraceus, Aspergillussteynii and Aspergilluswesterdijkiae) makes difficult discrimination among them and with respect to the other species included in the same section unable to produce OTA. In this work, PCR assays specific for the OTA-producing species, A. ochraceus, A. steynii and A. westerdijk... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2887573 Fri, 25 Sep 2009 23:00:00 +0100 2887573 http://www.medworm.com/index.php?rid=2887575&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818520%26dopt%3DAbstract This study investigated accumulation of Vibrio parahaemolyticus in Zhe oyster (Crassostrea plicatula) from culture water and effectiveness of frozen and chilled storage on reducing V. parahaemolyticus in oysters. Freshly harvested oysters were placed in artificial seawater containing V. parahaemolyticus (10(4)CFU/mL) at 16, 20, 26, and 32 degrees C for 96h. Contaminated oysters were stored at chilled temperatures (0, 5, and 15 degrees C) and frozen at -18 and -30 degrees C and changes of V. parahaemolyticus populations in oysters were determined using the most probable number (MPN) method. Accumulations of V. parahaemolyticus in C.plicatula reached the peaks at 6.66 (32 degrees C), 5.72 (26 degrees C), 5.04 (20 degrees C), 4.72 (16 degrees C) log MPN/g after 32h in contaminated artificial ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2887575 Mon, 21 Sep 2009 23:00:00 +0100 2887575 http://www.medworm.com/index.php?rid=2878587&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19811846%26dopt%3DAbstract Authors: Arroyo C, Condón S, Pagán R In the present study the influence of various environmental and physiological factors on the heat resistance of Enterobacter sakazakii (Cronobacter) have been investigated. Our results demonstrated that the heat resistance of E. sakazakii depended on the strain studied, the growth conditions - phase and temperature - the characteristics of treatment medium and the recovery conditions. The strain STCC 858 (ATCC type strain 29544) showed maximum heat resistance among the strains tested and it was selected for the further study. Stationary-phase cells grown between 20 and 37 degrees C (mean D(60)=0.9min) resulted to be more resistant than cells grown at 10 degrees C (D(60)=0.2min). Resistance decreased when the treatment medium pH was lower t... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2878587 Mon, 21 Sep 2009 23:00:00 +0100 2878587 http://www.medworm.com/index.php?rid=2847360&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19786312%26dopt%3DAbstract Authors: Jasson V, Sampers I, Botteldoorn N, López-Gálvez F, Baert L, Denayer S, Rajkovic A, Habib I, De Zutter L, Debevere J, Uyttendaele M A comparative study examining Bolton broth and Preston broth for enrichment and reliable detection of Campylobacter jejuni (both healthy and freeze stressed cells) was performed. Tested as pure cultures, Bolton broth enabled faster resuscitation and growth of C. jejuni compared to Preston broth. When C. jejuni was co-incubated with extended-spectrum-beta-lactamase (ESBL) producing Escherichia coli isolated from Belgian poultry meat preparations, the latter dominated in the Bolton enrichment broth and crowded the mCCDA plates. This resulted in the inability to recover C. jejuni by ISO 10272-1:2006 standard method. Preston broth did not su... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2847360 Tue, 15 Sep 2009 23:00:00 +0100 2847360 http://www.medworm.com/index.php?rid=2839496&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19783060%26dopt%3DAbstract Authors: Sterr Y, Weiss A, Schmidt H Spontaneous fermented sourdoughs prepared from five amaranth flours were investigated for the presence of lactic acid bacteria predominating the autochthonous microbiota and thus may be suitable as starter cultures. The doughs were fermented with daily back-slopping on a laboratory scale at 30 degrees C for 10days. Each day, pH-values and total titratable acidity degrees were determined and samples were analyzed for lactic acid bacteria and yeasts by cultural methods. The identity of the strains was tracked with randomly amplified polymorphic DNA-PCR during fermentation. Taxonomic identity of the strains was revealed by sequence analysis of 16S rDNA. Sugar and organic acid profiles of fermented doughs were determined with HPLC. The strains Lactobaci... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2839496 Mon, 14 Sep 2009 23:00:00 +0100 2839496 http://www.medworm.com/index.php?rid=2832506&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775768%26dopt%3DAbstract Authors: Lappe R, Motta AS, Sant'anna V, Brandelli A The ability of the bacteriocin cerein 8A to inhibit Salmonella Enteritidis in combination with EDTA and sodium lactate was investigated. Salmonella Enteritidis was incubated with combinations of cerein 8A (3200AU/mL) and EDTA (20, 50, 100mmol/L) or sodium lactate (200mmol/L). All treatments caused a significant reduction in the OD(600) values of Salmonella Enteritidis cultures. The addition of cerein 8A plus EDTA resulted in higher inhibition in comparison with the bacteriocin alone; the greater the concentration of EDTA, the greater the inhibitory effect. The combination of cerein 8A plus 100mmol/L EDTA results in a more efficient treatment to reduce the number of viable cells of Salmonella Enteritidis. The combination of cerein 8A ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2832506 Fri, 11 Sep 2009 23:00:00 +0100 2832506 http://www.medworm.com/index.php?rid=2832507&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775767%26dopt%3DAbstract Authors: Nissen L, Chingwaru W, Sgorbati B, Biavati B, Cencic A In interaction studies with the host intestine, the use of the appropriate gut functional cell model is essential. Therefore, we examined the protective properties of selected lactobacilli in a newly established intestinal cell model. Bacteria were cocultured with the pig small intestinal epithelial cells (PSIc1) and pig blood monocytes (PoM2) in a functional intestinal cell model. Intercellular intestinal integrity was measured by transepithelial electrical resistance (TER), before and after coculture with selected bacterial strains. All selected bacterial strains showed important gut health promoting activity by: enhancing the intestinal integrity and increasing metabolic activity of intestinal cells. Stimulation of immu... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2832507 Tue, 08 Sep 2009 23:00:00 +0100 2832507 http://www.medworm.com/index.php?rid=2810327&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762101%26dopt%3DAbstract Authors: Mols M, Pier I, Zwietering MH, Abee T Both the growth and stress survival of two model Bacillus cereus strains, ATCC 14579 and ATCC 10987, were tested in three different conditions varying in oxygen availability, i.e., aerobic, microaerobic and anaerobic conditions. Both B. cereus strains displayed highest growth rates and yields under aerobic conditions, whereas the microaerobic and anaerobic cultures showed similar reduced growth performances. The cells grown and exposed microaerobically and anaerobically were more resistant to heat and acid than cells that were cultured and exposed aerobically. On the other hand, the anaerobically grown cells were more sensitive to hydrogen peroxide compared to the (micro)aerobically grown cells. The increased heat- and acid-induced inactiv... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2810327 Tue, 08 Sep 2009 23:00:00 +0100 2810327 http://www.medworm.com/index.php?rid=2775369&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19733930%26dopt%3DAbstract This study shows that irradiation effectively reduces viable E. coli cells internalized in lettuce, and decontamination is not influenced by lettuce variety. Ionizing irradiation effectively reduced the population of internalized pathogen in a dose-dependent manner and could be used as an effective killing step to mitigate the risk of foodborne disease outbreaks. PMID: 19733930 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2775369 Wed, 26 Aug 2009 23:00:00 +0100 2775369 http://www.medworm.com/index.php?rid=2762233&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19720415%26dopt%3DAbstract Authors: Pablos M, Rodríguez-Calleja JM, Santos JA, Otero A, García-López ML Aeromonas-associated cases of gastroenteritis are generally considered waterborne. The purpose of this study was to evaluate the potential microbiological risk associated with the presence of these bacteria in public drinking water. Over a period of one year, 132 drinking-water samples were monitored in León (NW of Spain, 137,000 inhabitants) for mandatory drinking-water standards and the occurrence of Aeromonas spp. Samples were taken at the municipal water treatment plant, one storage facility, and two public artesian drinking-water fountains. Because of low numbers of coliforms or Clostridium perfringens, the non-compliance rate with microbial standards was 3.8% whereas the percentage of... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2762233 Fri, 21 Aug 2009 23:00:00 +0100 2762233 http://www.medworm.com/index.php?rid=2762232&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19723600%26dopt%3DAbstract This study shows that the prevalence of enteropathogenic Yersinia is high and both enteropathogenic Y. enterocolitica and Y. pseudotuberculosis are common findings in tonsils of wild boars in Switzerland. PMID: 19723600 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2762232 Fri, 21 Aug 2009 23:00:00 +0100 2762232 http://www.medworm.com/index.php?rid=2752628&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19717202%26dopt%3DAbstract Authors: Zhang H, Shen Y, Weng P, Zhao G, Feng F, Zheng X Microemulsions are colloidal nanodispersions of oil and water stabilized by an interfacial film of surfactant molecules, typically in conjunction with a cosurfactant. There is a limited number of reports in the literature on microemulsion use for antimicrobial purposes. The physicochemical characterization of a food-grade fully dilutable microemulsion system with glycerol monolaurate (GML) as oil, organic acids as cosurfactant, Tween 80 as surfactant, and the antimicrobial activities against Escherichia coli and Staphylococcus aureus have been studied in this paper. The influence of organic acids on oil solubilization was clearly reflected in the phase behavior of these systems. Propionic acid demonstrated the greatest capabilit... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2752628 Tue, 18 Aug 2009 23:00:00 +0100 2752628 http://www.medworm.com/index.php?rid=2752629&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19717201%26dopt%3DAbstract Authors: Mayr C, Strohe G, Contzen M In the course of a rotavirus outbreak in a mother and child sanatorium 74 food samples from the sanatorium kitchen were taken and tested for rotavirus. Rotavirus particles were isolated from 25 g food samples by a simple method including ultrafiltration, originally designed for the detection of norovirus in various food matrices. Rotavirus was successfully detected in a sample of potato stew by conventional RT-seminested-PCR. Sequence comparison of the amplification products obtained from the potato stew and a stool sample from an infected child verified that the two viruses were identical. PMID: 19717201 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2752629 Wed, 12 Aug 2009 23:00:00 +0100 2752629 http://www.medworm.com/index.php?rid=2703251&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19679367%26dopt%3DAbstract Authors: Magnino S, Colin P, Dei-Cas E, Madsen M, McLauchlin J, Nöckler K, Prieto Maradona M, Tsigarida E, Vanopdenbosch E, Van Peteghem C The consumption of a wide variety of species of reptiles caught from the wild has been an important source of protein for humans world-wide for millennia. Terrapins, snakes, lizards, crocodiles and iguanas are now farmed and the consumption and trade of their meat and other edible products have recently increased in some areas of the world. Biological risks associated with the consumption of products from both farmed and wild reptile meat and eggs include infections caused by bacteria (Salmonella spp., Vibrio spp.), parasites (Spirometra, Trichinella, Gnathostoma, pentastomids), as well as intoxications by biotoxins. For crocodiles, Salmonella ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2703251 Tue, 11 Aug 2009 23:00:00 +0100 2703251 http://www.medworm.com/index.php?rid=2700012&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19674803%26dopt%3DAbstract Authors: Nauta MJ, van der Wal FJ, Putirulan FF, Post J, van de Kassteele J, Bolder NM "Testing and scheduling" has been proposed as a strategy for control of Campylobacter in broiler meat. By this strategy, flocks with high numbers of Campylobacter in fecal samples would be diverted away from fresh meat production at the entrance of the broiler meat processing plant. Risk assessment studies suggest that this would effectively decrease human health risks, if these flocks are responsible for the meat products with the highest Campylobacter numbers. To investigate the effect of this control strategy, the numbers of Campylobacter were determined in fecal samples from transport containers, and in cecal and breast meat samples from birds in 62 broiler chicken flocks. Results from direct pla... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2700012 Sun, 09 Aug 2009 23:00:00 +0100 2700012 http://www.medworm.com/index.php?rid=2675368&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19651454%26dopt%3DAbstract Authors: Pennacchia C, Ercolini D, Villani F Brochothrix thermosphacta is a psychrotrophic species commonly involved in the spoilage of meat and often recognized as the dominant organism causing off-flavours. The knowledge of the genera/species affecting meat spoilage is necessary to define a successful method for food preservation. The aim of this study was to develop a Real-Time (RTi-) PCR method for the species-specific detection of B. thermosphacta and to evaluate a RTi-PCR approach for its enumeration in fresh and spoiled beef, avoiding the culturing steps. The specificity of the primers designed on the basis of the 16S rRNA gene sequences of B. thermosphacta was tested using the DNA extracted from strains belonging to bacterial species usually associated with meat. The RTi-PCR as... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2675368 Fri, 31 Jul 2009 23:00:00 +0100 2675368 http://www.medworm.com/index.php?rid=2675366&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19651456%26dopt%3DAbstract This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive the ionising radiation stress. PMID: 19651456 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2675366 Thu, 16 Jul 2009 23:00:00 +0100 2675366 http://www.medworm.com/index.php?rid=2675367&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19651455%26dopt%3DAbstract Authors: Robert H, Gabriel V, Fontagné-Faucher C The lactic acid microflora of nine traditional wheat sourdoughs from the Midi-Pyrénées area (South western France) was previously isolated and preliminary characterized using conventional morphological and biochemical analysis. However, such phenotypic methods alone are not always reliable and have a low taxonomic resolution for identification of lactic acid bacteria species. In the present study, a total of 290 LAB isolates were identified by PCR amplification using different sets of specific primers in order to provide a thorough characterization of the lactic flora from these traditional French sourdoughs. Overall, the LAB isolates belonged to 6 genera: Lactobacillus (39%, 8 species), Pediococcus (38%, 1 species), Leuco... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2675367 Sat, 11 Jul 2009 23:00:00 +0100 2675367 http://www.medworm.com/index.php?rid=2675365&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654071%26dopt%3DAbstract Authors: Whitehead KA, Benson P, Verran J The accurate monitoring of surface cleanliness in terms of bacterial contamination is usually carried out using methods such as plate counts or replica plating. However these methods take at least eighteen hours to obtain results and do not determine the presence or amount of residual organic material on a surface, which may interfere with cleaning and disinfection. This work describes the application of fluorescent stains to cells (Listeria monocytogenes) and food soil (solubilized whey) to optimize a dual staining method that can be used in the quantitative analysis of surface cleanability. Seven different stains were tested at a range of concentrations (0.3%-0.001 mg/ml) and application methods. The best stain combination for differential st... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2675365 Sat, 11 Jul 2009 23:00:00 +0100 2675365 http://www.medworm.com/index.php?rid=2646203&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19632733%26dopt%3DAbstract Authors: Tronchoni J, Gamero A, Arroyo-López FN, Barrio E, Querol A Yeasts with a high fructose consumption capability are very important for winemakers to solve problems associated with sluggish or stuck fermentations causing undesirable sweetness in wines. In the present study, we analyze the kinetics of glucose and fructose consumption during wine fermentations performed at low (12 degrees C) and high (28 degrees C) temperatures by twelve different yeast strains belonging to the species Saccharomyces cerevisiae, S. bayanus var. uvarum, S. kudriavzevii as well as interspecific Saccharomyces hybrids. Different mathematical equations (sigmoid, exponential and linear decay functions) were used to fit, by means of linear and nonlinear regressions, the sugar degradation along the fer... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2646203 Sat, 11 Jul 2009 23:00:00 +0100 2646203 http://www.medworm.com/index.php?rid=2646202&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19632734%26dopt%3DAbstract Authors: Al-Zoreky NS Food-borne diseases such as listeriosis and diseases caused by the emergence of multi-drug resistant pathogens (e.g. Staphylococcus aureus) are globally recognized as environmental hazards to the food supply and human health. Natural inhibitors for pathogenic microorganisms have been explored in many plants. The antimicrobial activity against some food-borne pathogens by various extracts from pomegranate fruit peels was evaluated using both in vitro (agar diffusion) and in situ (food) methods. The 80% methanolic extract of peels (WME) was a potent inhibitor for Listeria monocytogenes, S. aureus, Escherichia coli and Yersinia enterocolitica. The minimum inhibitory concentration (MIC) of WME against Salmonella enteritidis was the highest (4 mg/ml). WME afforded >... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2646202 Sat, 11 Jul 2009 23:00:00 +0100 2646202 http://www.medworm.com/index.php?rid=2622135&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19616336%26dopt%3DAbstract Authors: Kiwaki M, Sato T The minimum inhibitory concentrations (MICs) of 17 antimicrobials for 26 Bifidobacterium breve strains of various origins were determined by broth microdilution. MIC distributions for 17 antimicrobials except streptomycin and tetracycline were unimodal for all strains tested, whereas bimodal distributions were observed for streptomycin and tetracycline. The probiotic strain B. breve strain Yakult showed intrinsic susceptibility to all antimicrobials except streptomycin to which the strain showed an atypically higher MIC of >256mug/ml. Because this strain is a commercial strain, which is often ingested by many consumers on a daily basis, it is very important to determine the genetic basis for streptomycin resistance of this strain. Molecular analysis reveale... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2622135 Tue, 30 Jun 2009 23:00:00 +0100 2622135 http://www.medworm.com/index.php?rid=2592251&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19589610%26dopt%3DAbstract Authors: Jaeger H, Schulz A, Karapetkov N, Knorr D The inactivation of Lb. rhamnosus by pulsed electric field treatment (PEF) was studied in different fractions of raw milk and Ringer solution in order to evaluate the protective effect of nutrient rich media in comparison to aqueous buffer solutions. Apart from monitoring of culturability, analysis of the physiological fitness of Lb. rhamnosus was conducted aiming to identify sublethally damaged cells. Therefore, flow cytometry and a selective medium plating technique were used and compared to each other. The goal of the study was to apply three different parameters describing the physiological fitness of the model organism Lb. rhamnosus after PEF treatment such as culturability, membrane permeability and metabolic activity depending o... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2592251 Fri, 26 Jun 2009 23:00:00 +0100 2592251 http://www.medworm.com/index.php?rid=2580789&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19577815%26dopt%3DAbstract Authors: Ongol MP, Asano K Ghee is widely produced from a traditional fermented butter-like product named mashita in western Uganda. However, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. The aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. The most commonly identified species of lactic acid bacteria (LAB) in mashita using culture-dependent techniques were Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus plantarum and Lactobacillus perolens constituting 37.3%, 10.1%, 8.1% and 7.7% of total bacterial colonies isolated respectively. L. paracasei was the only bacterial species identified in all mashita samples by cultu... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2580789 Tue, 16 Jun 2009 23:00:00 +0100 2580789 http://www.medworm.com/index.php?rid=2477449&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%2520%28%2522Int%2520J%2520Food%2520Microbiol%2522%255Bta%255D%29%2520AND%2520%25221900%252F01%252F01%252000.00%2522%255BEDAT%255D%253A%25222009%252F06%252F15%252023.15%2522%255BEDAT%255D%29 4242 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Int J Food Microbiol"[ta] These pubmed results were generated on 2009/06/15PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2477449 Tue, 16 Jun 2009 03:15:07 +0100 2477449 http://www.medworm.com/index.php?rid=2260419&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19268382%26dopt%3DAbstract Authors: Nguefack J, Dongmo JB, Dakole CD, Leth V, Vismer HF, Torp J, Guemdjom EF, Mbeffo M, Tamgue O, Fotio D, Zollo PH, Nkengfack AE The food preservative potential of essential oils from three aromatic plants Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris and their fractions was investigated against two mycotoxigenic strains each of Aspergillus ochraceus, Penicillium expansum and P. verrucosum. The fungicidal activity was determined and expressed as a Number of Decimal Reduction of the colony forming units per ml (NDR cfu). The influence of pH variation on this activity was studied. The NDR cfu varied with the essential oils and its concentration, the pH of the medium and the strain tested. The essential oils from O. gratissimum exhibited the highest activity against th... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2260419 Wed, 04 Mar 2009 05:00:00 +0100 2260419 http://www.medworm.com/index.php?rid=2249394&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19264370%26dopt%3DAbstract Authors: Zoumpopoulou G, Tsakalidou E, Dewulf J, Pot B, Grangette C Intestinal epithelial cells (IECs) provide a primary physical barrier against commensal and pathogenic bacteria, but the influence of IECs in the regulation of the associated mucosal immune system remains largely unknown. The network of dendritic cells (DCs) in the vicinity of IECs is known to play a crucial role in the regulation of gut homeostasis. We investigated the cross-talk between murine IECs (m-IC(cl2) cell line), bone marrow derived DCs and different bacteria using an in vitro Transwell((R)) co-culture model. IECs responded poorly to different Gram-positive lactic acid bacteria (LAB) and to a Staphylococcus aureus strain. In contrast two Escherichia coli strains, including the probiotic strain Nissle 1917, st... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2249394 Tue, 03 Mar 2009 05:00:00 +0100 2249394 http://www.medworm.com/index.php?rid=2233609&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19250693%26dopt%3DAbstract Authors: Dantigny P, Nanguy SP In predictive mycology, most of the studies have been concerned with the influence of some environmental factors on fungal growth and production of mycotoxins, at steady-state. However, fluctuating conditions, interactions between organisms, and the physiological state of the organisms may also exert a profound influence on fungal responses in food and in the environment. In the laboratory, fungal spores are widely used as a biological material. They are produced under optimal conditions then, partially re-hydrated for obtaining standardized spore suspensions. In real conditions, spores are produced under suboptimal conditions and can be submitted to various stresses prior to their germination. It was illustrated how the sporulation/post-sporulation condi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2233609 Thu, 12 Feb 2009 05:00:00 +0100 2233609 http://www.medworm.com/index.php?rid=2233607&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19251335%26dopt%3DAbstract Authors: Ravyts F, Vrancken G, D'Hondt K, Vasilopoulos C, De Vuyst L, Leroy F Five species of meat-borne, coagulase-negative staphylococci were screened for their in vitro production of 3-methyl-1-butanol. The highest production level was encountered for Staphylococcus sciuri alphaSG2, despite its poor growth. With respect to Staphylococcus species that are generally applied in sausage starter cultures, production of 3-methyl-1-butanol was higher with Staphylococcus xylosus 3PA6 than with Staphylococcus carnosus 833. Mathematical modelling was used to link the kinetics of 3-methyl-1-butanol production by S. xylosus 3PA6 and S. carnosus 833 in meat simulation medium to bacterial growth and environmental factors, in casu temperature and pH. The specific production rate of 3-methyl-1-buta... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2233607 Thu, 12 Feb 2009 05:00:00 +0100 2233607 http://www.medworm.com/index.php?rid=2233608&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19251334%26dopt%3DAbstract Authors: Bernbom N, Jelle B, Brogren CH, Vogensen FK, Nørrung B, Licht TR The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods. The culturable microbiota was in all treatments dominated by lactic acid bacteria and coliforms and no changes in the rat comme... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2233608 Wed, 11 Feb 2009 05:00:00 +0100 2233608 http://www.medworm.com/index.php?rid=2233606&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19254814%26dopt%3DAbstract Authors: Vandekinderen I, Devlieghere F, Van Camp J, Kerkaert B, Cucu T, Ragaert P, De Bruyne J, De Meulenaer B Chlorine dioxide (ClO(2)) is a strong oxidizing agent that can be applied in solution as well as in the gaseous state. It has bactericidal, fungicidal and viricidal properties. Several food-related microorganisms, including Gram-negative and Gram-positive bacteria, yeasts, mould spores and Bacillus cereus spores were tested for their susceptibility to 0.08 mg/L gaseous ClO(2) during 1 min at a relative humidity of 90%. In this screening, the resistance of the different groups of microorganisms towards gaseous ClO(2) generally increased in the order Gram-negative bacteria, Gram-positive bacteria, yeasts and mould spores and Bacillus cereus spores. With this treatment, reductio... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2233606 Wed, 11 Feb 2009 05:00:00 +0100 2233606 http://www.medworm.com/index.php?rid=2222860&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19246113%26dopt%3DAbstract Authors: Songré-Ouattara LT, Mouquet-Rivier C, Icard-Vernière C, Rochette I, Diawara B, Guyot JP Fermentation and starch hydrolysis of a pre-cooked pearl millet-groundnut (MG) slurry inoculated with amylolytic lactic acid bacteria (ALAB) or by back slopping was investigated as a substitute for the addition of malt to prepare infant gruels. The ALAB collection strain Lb. plantarum A6, and the endogenous microflora provided by back slopping were more efficient in acidifying and partially hydrolysing starch in the MG slurry than Lb. plantarum 6.1, isolated from the traditional process in Burkina Faso. Large amounts of maltotriose and maltotetraose accumulated in slurry fermented by strain A6. No accumulation of maltose was observed, which could be an advantage to prevent the gro... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2222860 Fri, 06 Feb 2009 05:00:00 +0100 2222860 http://www.medworm.com/index.php?rid=2222861&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19246112%26dopt%3DAbstract Authors: Arroyo-López FN, Orlić S, Querol A, Barrio E The effects of temperature, pH and sugar concentration (50% glucose+50% fructose) on the growth parameters of Saccharomyces cerevisiae T73, S. kudriavzevii IFO 1802(T) and the hybrid strain S. cerevisiae x S. kudriavzevii W27 were studied by means of response surface methodology based in a central composite circumscribed design. Lag phase could not be properly modelled in the wine model system, where yeasts started the fermentation in few hours after inoculation. In the case of the maximum specific growth rate (micro(max)), the temperature was the most important variable for three yeasts, although the effects of sugar concentration (in T73 and W27) and pH (W27 and 1802) were also significant (p<0.05). The only retained... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2222861 Thu, 05 Feb 2009 05:00:00 +0100 2222861 http://www.medworm.com/index.php?rid=2216934&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19237220%26dopt%3DAbstract In this study, F. poae specific TaqMan assays and mycotoxin analysis were performed on 48 asymptomatically contaminated wheat grain samples obtained from six different locations in northern Poland in 2006 and 2007. TaqMan assays revealed the presence of F. poae DNA in all samples analyzed, however the amounts of target DNA between the samples differed. Mycotoxin analysis revealed the presence of 13 toxins in the grain analyzed, however only ENN B and B1 were detected at quantifiable concentrations. A significant positive correlation was revealed between F. poae DNA (R=0.75) and monthly mean rainfalls recorded in May (a month before wheat anthesis) in both years. Additionally, a significant positive correlation was found between levels of ENN B+B1 (R=0.49) and rainfalls in May, however, no ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2216934 Thu, 05 Feb 2009 05:00:00 +0100 2216934 http://www.medworm.com/index.php?rid=2216933&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239978%26dopt%3DAbstract This study examined the utilization patterns of key carbon sources (CS, 24: including key sugars, amino acids and fatty acids) in maize by strains of Aspergillus flavus and Fusarium verticillioides under different water activity (a(w), 0.87-0.98 a(w)) and temperature (20-35 degrees C) values and compared the niche overlap indices (NOI) that estimate the in vitro CS utilization profiles [Wilson, M., Lindow, S.E., 1994. Coexistence among epiphytic bacterial populations mediated through nutritional resource partitioning. Applied and Environmental Microbiology 60, 4468-4477.]. The ability to grow in these key CS in minimal media was studied for 120 h in 12 h steps. The NOI was calculated for inter-species (F. verticillioides-A. flavus) and for intra-species (A. flavus-A. flavus) using CS utili... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2216933 Wed, 04 Feb 2009 05:00:00 +0100 2216933 http://www.medworm.com/index.php?rid=2211179&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19232766%26dopt%3DAbstract 15th Meeting of the Club des Bactéries Lactiques Rennes, 13-15 November 2007. Int J Food Microbiol. 2009 Feb 3; Authors: Le Loir Y PMID: 19232766 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2211179 Tue, 03 Feb 2009 05:00:00 +0100 2211179 http://www.medworm.com/index.php?rid=2211180&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19230999%26dopt%3DAbstract The objective of this study was to examine the Saccharomyces and non-Saccharomyces yeast populations involved in a spontaneous fermentation of a traditional high sugar must (Vino cotto) produced in central Italy. Molecular identification of a total of 78 isolates was achieved by a combination of PCR-RFLP of the 5.8S ITS rRNA region and sequencing of the D1/D2 domain of the 26S rRNA gene. In addition, the isolates were differentiated by RAPD-PCR. Only a restricted number of osmotolerant yeast species, i.e. Candida apicola, Candida zemplinina and Zygosaccharomyces bailii, were found throughout all the fermentation process, while Saccharomyces cerevisiae prevailed after 15 days of fermentation. A physiological characterization of isolates was performed in relation to the resistance to osmotic... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2211180 Fri, 30 Jan 2009 05:00:00 +0100 2211180 http://www.medworm.com/index.php?rid=2211177&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19232768%26dopt%3DAbstract Authors: Pina-Pérez MC, Silva-Angulo AB, Rodrigo D, Martínez-López A With a view to extending the shelf-life and enhancing the safety of liquid whole egg/skim milk (LWE-SM) mixed beverages, a study was conducted with Bacillus cereus vegetative cells inoculated in skim milk (SM) and LWE-SM beverages, with or without antimicrobial cocoa powder. The beverages were treated with Pulsed Electric Field (PEF) technology and then stored at 5 degrees C for 15 days. The kinetic results were modeled with the Bigelow model, Weibull distribution function, modified Gompertz equation, and Log-logistic models. Maximum inactivation registered a reduction of around 3 log cycles at 40 kV/cm, 360 micros, 20 degrees C in both the SM and LWE-SM beverages. By contrast, in the beverages suppleme... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2211177 Fri, 30 Jan 2009 05:00:00 +0100 2211177 http://www.medworm.com/index.php?rid=2211178&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19232767%26dopt%3DAbstract Authors: Giannino ML, Marzotto M, Dellaglio F, Feligini M The bacterial populations of raw milk employed for the production of Fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. Total microbial DNA was isolated from milk and curd samples and used as template in Polymerase Chain Reaction (PCR) to study the hypervariable V3 region of the bacterial 16S rRNA gene and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). Representative bands of DGGE patterns were sequenced for identification purposes. The use of universal primer for PCR-DGGE allowed the description of the bacterial community, not only for the presence of lactic acid bacteria, but also for other adventitious... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2211178 Thu, 29 Jan 2009 05:00:00 +0100 2211178 http://www.medworm.com/index.php?rid=2190944&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217681%26dopt%3DAbstract Authors: Mahmoud BS The purpose of this investigation was to study the inactivation effect of X-ray treatments on inoculated Vibrio vulnificus in pure culture, half shell and whole shell oysters to achieve a 5.0 log reduction, which is recommended by the Interstate Shellfish Sanitation Conference and the Food and Drug Administration. A mixed culture of three V. vulnificus strains was used to prepare the pure culture and inoculated oysters. The pure culture and inoculated oysters were treated with 0.0, 0.1, 0.5, 0.75, 1.0, 1.5, 2.0, and 3.0 kGy X-ray at 22 degrees C and 50-60% relative humidity. Surviving bacterial populations in the pure culture and inoculated oysters were enumerated using overlay-plating method [with a non-selective media (trypticase soy agar) followed by a selective ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190944 Thu, 29 Jan 2009 05:00:00 +0100 2190944 http://www.medworm.com/index.php?rid=2190947&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217182%26dopt%3DAbstract Authors: Di Cagno R, Surico RF, Minervini G, De Angelis M, Rizzello CG, Gobbetti M Strains of Lactobacillus curvatus, Leuconostoc mesenteroides, Lactobacillus plantarum and Weissella confusa were identified from raw red and yellow peppers (RYPs) by partial 16S rRNA gene sequence and subjected to typing by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis. L. plantarum PE21, L. curvatus PE4 and W. confusa PE36 were selected based on the kinetics of growth and acidification, and used as the autochthonous mixed starter for the fermentation of RYPs. A protocol which included blanching at 85 degrees C for 2 min, fermentation at 35 degrees C for 15 h in brine (1%, w/v), and heat treatment at 85 degrees C for 15 min, followed by storage at room temperature for 30 ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190947 Tue, 27 Jan 2009 05:00:00 +0100 2190947 http://www.medworm.com/index.php?rid=2190949&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217180%26dopt%3DAbstract Authors: Ferrer J, Prats C, López D, Vives-Rego J Predictive microbiology is the area of food microbiology that attempts to forecast the quantitative evolution of microbial populations over time. This is achieved to a great extent through models that include the mechanisms governing population dynamics. Traditionally, the models used in predictive microbiology are whole-system continuous models that describe population dynamics by means of equations applied to extensive or averaged variables of the whole system. Many existing models can be classified by specific criteria. We can distinguish between survival and growth models by seeing whether they tackle mortality or cell duplication. We can distinguish between empirical (phenomenological) models, which mathematically describe spe... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190949 Sat, 24 Jan 2009 05:00:00 +0100 2190949 http://www.medworm.com/index.php?rid=2190948&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217181%26dopt%3DAbstract Authors: Williams LK, Jørgensen F, Grogono-Thomas R, Humphrey TJ Isolation of Campylobacter spp. using enrichment culture is time consuming and complex. Reducing the time taken to confirm the presence or absence of Campylobacter spp. would have many advantages for diagnostic, commercial and research applications. Rapid techniques such as real-time PCR can detect campylobacters from complex samples but blood in enrichment culture can inhibit the PCR reaction, if applied directly to enriched samples. The aim of this study was to investigate the effect of blood in enrichment culture on the isolation of campylobacters from chicken caeca, carcass rinses and bootsock (gauze sock walked through a broiler chicken house) samples using Bolton broth. The effect of incubation temperature (37 ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190948 Sat, 24 Jan 2009 05:00:00 +0100 2190948 http://www.medworm.com/index.php?rid=2190945&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217680%26dopt%3DAbstract Authors: Jiménez-Martí E, Zuzuarregui A, Ridaura I, Lozano N, Del Olmo M Throughout wine production yeast cells are affected by a plethora of stress conditions that compromise their ability to carry out the whole process. In recent years important knowledge about the mechanisms involved in stress response in both laboratory and wine yeast strains has been obtained. Several studies have indicated that a correlation exists between stress resistance, expression of stress response genes and fermentative behaviour. In this work we introduce several genetic manipulations in two genes induced by several stress conditions: HSP26 (which encodes a heat shock protein) and YHR087W (encoding a protein of unknown function) in two different wine yeasts, ICV16 and ICV27. These manipulations ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190945 Sat, 24 Jan 2009 05:00:00 +0100 2190945 http://www.medworm.com/index.php?rid=2190943&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217682%26dopt%3DAbstract Authors: Jaquet M, Rochat I, Moulin J, Cavin C, Bibiloni R The impact of a moderate consumption of an instant coffee on the general composition of the human intestinal bacterial population was assessed in this study. Sixteen (16) healthy adult volunteers consumed a daily dose of 3 cups of coffee during 3 weeks. Faecal samples were collected before and after the consumption of coffee, and the impact of the ingestion of the product on the intestinal bacteria as well as the quantification of specific bacterial groups was assessed using nucleic acid-based methods. Although faecal profiles of the dominant microbiota were not significantly affected after the consumption of the coffee (Dice's similarity index=92%, n=16), the population of Bifidobacterium spp. increased after the 3-week test p... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190943 Fri, 23 Jan 2009 05:00:00 +0100 2190943 http://www.medworm.com/index.php?rid=2190946&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217679%26dopt%3DAbstract Authors: Gündüz GT, Gönül SA, Karapinar M The antimicrobial activity of myrtle leaves (Myrtus communis) oil was tested against the nalidixic acid resistant strain of Salmonella Typhimurium ATCC 13311. An inoculum (100 microl, ca.10(8) cfu/ml) was deposited on the skin of whole tomatoes and 10 g of shredded iceberg lettuce, dried for 2 h at 22 degrees C and held for 22 h at 4 degrees C before treatments. Inoculated iceberg lettuce (3.51-3.99 log cfu/g) and tomatoes (3.47-4.86 log cfu/tomato) were treated with three different washing procedures for 5, 10, 15 and 20 min; washing with sterile distilled water (control), washing with three different concentrations of myrtle leaves oil and the last treatment was a combination of washing with myrtle leaves oil and then rins... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2190946 Thu, 22 Jan 2009 05:00:00 +0100 2190946 http://www.medworm.com/index.php?rid=2177658&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19203811%26dopt%3DAbstract This study characterizes the effects of an acidic pH and an emulsified oil-in-water phase in a culture medium on the behavior of Listeria monocytogenes. Two strains were tested, Scott A and CIP 78.39, and exhibited similar responses to growth media. First of all, the results showed that the emulsified oil phase had no effect on growth kinetics, whereas acidification of the initial pH (from 7.2 to 5.2) reduced both growth rates and growth yields. Secondly, physicochemical cell surface properties were evaluated. Growth in an emulsion resulted in a more marked increase in hydrophobicity in neutral than in acidic media, whereas the electrical charge remained unchanged. Furthermore, growth in acidic media - emulsified or not - induced a reduction in hydrophobicity as well as in the negative cha... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2177658 Wed, 21 Jan 2009 05:00:00 +0100 2177658 http://www.medworm.com/index.php?rid=2160593&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19187994%26dopt%3DAbstract Authors: Kingsley DH, Chen H The effects of pH (3-7), NaCl (0-6%), and temperature on pressure inactivation of hepatitis A virus (HAV) were determined. The HAV samples were treated at 400 MPa for 1 min at 5, 20, and 50 degrees C. Decreasing solution pH enhanced pressure inactivation of HAV. This enhanced inactivation effect was most evident at 20 degrees C. A baroprotective effect was observed for NaCl concentrations from 1 to 6%. For example, a treatment of 400 MegaPascals (MPa) for 1 min at 50 degrees C reduced the HAV titers by 4.0, 4.1, 1.3 and 0.4 log plaque forming units (PFU)/ml for NaCl concentrations of 0, 1, 3, and 6%, respectively. Overall, increasing the treatment temperature enhanced pressure inactivation of HAV in the solutions. The pressure resistance of HAV in oysters w... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2160593 Mon, 19 Jan 2009 05:00:00 +0100 2160593 http://www.medworm.com/index.php?rid=2160592&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19187995%26dopt%3DAbstract Authors: Rossetti L, Carminati D, Zago M, Giraffa G A Qualified Presumption of Safety (QPS) approach was applied to dominant lactic acid bacteria (LAB) associated with Grana Padano cheese whey starters. Thirty-two strains belonging to Lactobacillus helveticus, Lactobacillus delbrueckii subsp. lactis, Streptococcus thermophilus, and Lactobacillus fermentum, and representing the overall genotypic LAB diversity associated with 24 previously collected whey starters [Rossetti, L., Fornasari, M.E., Gatti, M., Lazzi, C., Neviani, E., Giraffa, G., 2008. Grana Padano cheese whey starters: microbial composition and strain distribution. International Journal of Food Microbiology 127, 168-171], were analyzed. All L. helveticus, L. delbrueckii subsp. lactis, and S. thermophilus isolates were suscep... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2160592 Tue, 13 Jan 2009 05:00:00 +0100 2160592 http://www.medworm.com/index.php?rid=2160591&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19187996%26dopt%3DAbstract Authors: Haakensen M, Schubert A, Ziola B Identification of the beer-spoilage Lactobacillus and Pediococcus bacteria has largely taken two approaches; identification of spoilage-associated genes or identification of specific species of bacteria regardless of ability to grow in beer. The problem with these two approaches is that they are either overly inclusive (i.e., detect all bacteria of a given species regardless of spoilage potential) or overly selective (i.e., rely upon individual, putative spoilage-associated genes). Our goal was to design a method to assess the ability of Lactobacillus and Pediococcus to spoil beer that is independent of speciation or genetic background. In searching for a method by which to differentiate between beer-spoilage bacteria and bacteria that cannot g... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2160591 Fri, 09 Jan 2009 05:00:00 +0100 2160591 http://www.medworm.com/index.php?rid=2156778&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19181411%26dopt%3DAbstract Authors: Kohut G, Adám AL, Fazekas B, Hornok L During cultivation of a wild type strain of Fusarium proliferatum on ammonium dihydrogen phosphate containing defined medium, expression levels of FUM1 and FUM8, members of the fumonisin biosynthesis gene cluster significantly increased when ammonium ion concentration of the culture medium decreased below 10 mM, indicating that N-depletion triggers the fumonisin biosynthesis genes. Deletion of Fphog1, a HOG-type MAP kinase gene resulted in further increases in FUM1 and FUM8 expression under nitrogen starvation (absence of any N-source) conditions. Fumonisin B1 (FB1) production paralleled with increased FUM gene expression: significant amounts of FB1 were measured in culture filtrates of the DeltaFphog1 deleted mutant after five days c... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2156778 Fri, 09 Jan 2009 05:00:00 +0100 2156778 http://www.medworm.com/index.php?rid=2156777&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19185379%26dopt%3DAbstract This study describes the examination of microbiological tests and the determination of OTA in boza temperature and time dependently. Prior to the analysis, physicochemical properties of the boza samples such as moisture, total acidity as lactic acid, pH, protein amount and viscosity were investigated. The incidence of total aerobic bacteria (TAB), lactic acid bacteria (LAB), coliforms, E.coli, Salmonella, S. aureus, B. cereus, yeast and moulds were examined. E.coli, Salmonella, S. aureus and B. cereus were not found in all boza samples. Initally, Aspergillus fumigatus; Acremonium sp.; Geotrichum candidum and Geotrichum capitatum were identified in the samples. Certain extraction techniques such as direct injection, liquid-liquid and solid phase (SP) were tried for the OTA analysis. The mos... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2156777 Fri, 09 Jan 2009 05:00:00 +0100 2156777 http://www.medworm.com/index.php?rid=2156776&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19185943%26dopt%3DAbstract This study addresses the question of constitutive versus regulated bacteriocin production on solid media in two different QS-regulated plantaricin-producing strains: Lactobacillus plantarum NC8 and L. plantarum WCFS1. Construction of knockout mutants for their respective regulatory operons revealed that bacteriocin production is controlled through a QS mechanism in both strains, on solid as well as in liquid media. These results could be extensible to other bacteriocins from LAB which are only produced on agar plates and not in broth cultures. Our findings suggest that QS-regulated bacteriocin production in LAB has evolved for competing on solid supports rather than in liquid media. In practice, this could be of major importance in vegetable fermentations, where the solid substrate itself ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2156776 Fri, 09 Jan 2009 05:00:00 +0100 2156776 http://www.medworm.com/index.php?rid=2149723&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19178974%26dopt%3DAbstract Authors: Greig JD, Ravel A Analysis of foodborne outbreak data is one approach to estimate the proportion of human cases of specific enteric diseases attributable to a specific food item (food attribution). Although we recognize that for a variety of reasons reported outbreaks represent only a small portion of all actual outbreaks, using outbreak data for food attribution is the only methodological approach where, theoretically, there is an actual direct link between the pathogen, its source and each infected person. The purpose of this study was to explore the usefulness of foodborne outbreak data extracted from publicly available international electronic reports and publications to provide estimates of food attribution, to derive and compare these estimates between regions, while imp... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2149723 Tue, 06 Jan 2009 05:00:00 +0100 2149723 http://www.medworm.com/index.php?rid=2149724&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19178973%26dopt%3DAbstract This study also highlights the potential of the strain Bc-10 as a treatment option for the regulation of LTB(4) production in vivo. PMID: 19178973 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2149724 Wed, 31 Dec 2008 05:00:00 +0100 2149724 http://www.medworm.com/index.php?rid=2141530&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19171404%26dopt%3DAbstract Authors: Zwietering MH In quantitative risk assessments a large variety of complexities can be found, from simple and deterministic to very extensive and stochastic. This publication advocates that both simple and complex approaches have their value and should be done in parallel. The simple analysis gives much insight and can help to detect main factors and potential errors in the complex analysis. Extensive analysis with increased complexity suggests better precision but might not increase the accuracy, due to the uncertainty in the additional parameters. However, complex analysis supplies more confidence in certain phenomena and might also increase insight. This is shown with two examples. The first is the effectiveness of sampling plans for powdered infant formula, for factories op... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2141530 Wed, 31 Dec 2008 05:00:00 +0100 2141530 http://www.medworm.com/index.php?rid=2137956&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19167125%26dopt%3DAbstract Authors: Heuvelink AE, van Heerwaarden C, Zwartkruis-Nahuis A, Tilburg JJ, Bos MH, Heilmann FG, Hofhuis A, Hoekstra T, de Boer E The present paper summarises the investigation of two different outbreaks of milk-associated Campylobacter enteritis in the Netherlands. In 2005, after a school trip to a dairy farm, 22 out of a group of 34 children developed diarrhoeal illness and Campylobacterjejuni was cultured from the stool samples of 11 of the cases. The illness was found to be epidemiologically associated with drinking raw milk during the farm visit; 86% of the cases could be explained by drinking raw milk. C.jejuni was also isolated from three of 10 faecal samples from dairy cattle collected at the farm. The human isolates and C.jejuni isolates from one of these three samples of cattl... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2137956 Wed, 31 Dec 2008 05:00:00 +0100 2137956 http://www.medworm.com/index.php?rid=2137955&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19167126%26dopt%3DAbstract Authors: Kim JY, Lee MY, Ji GE, Lee YS, Hwang KT Black raspberry juice was fermented to produce gamma-aminobutyric acid (GABA) using lactic acid bacteria (Lactobacillus brevis GABA 100) at different temperatures (25, 30, or 37 degrees C) and pHs (3.5, 4, 4.5, 5, 5.5, or 6) for 15 days. Concentrations of GABA in the juices were determined during fermentation using HPLC. GABA was produced continuously even if the viable bacterial counts markedly decreased. The fermentation at 30 degrees C generally showed higher production of GABA in the juices than those at 25 and 37 degrees C. The GABA in the juices fermented at 30 degrees C reached the maximum levels on the 12th day. The juices fermented at lower pH and lower temperature showed a lower degradation of monomeric anthocyanins. The result... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2137955 Wed, 31 Dec 2008 05:00:00 +0100 2137955 http://www.medworm.com/index.php?rid=2137954&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19168250%26dopt%3DAbstract The objective of this study was to compare the risk for consumers arising from their exposure to antibiotic resistant bacteria from meat of four different types (chicken, pork, beef and veal), distributed in four different product categories (fresh meat, frozen meat, dried raw meat products and heat-treated meat products). A semi-quantitative risk assessment model, evaluating each food chain step, was built in order to get an estimated score for the prevalence of Campylobacter spp., Enterococcus spp. and Escherichia coli in each product category. To assess human health impact, nine combinations of bacterial species and antimicrobial agents were considered based on a published risk profile. The combination of the prevalence at retail, the human health impact and the amount of meat or produc... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2137954 Wed, 31 Dec 2008 05:00:00 +0100 2137954 http://www.medworm.com/index.php?rid=2131480&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19162358%26dopt%3DAbstract Authors: Huang JQ, Jiang HF, Zhou YQ, Lei Y, Wang SY, Liao BS The effect of 2-chloroethyl phosphoric acid (CEPA) on aflatoxin biosynthesis, the expression of aflatoxin biosynthetic genes, reactive oxygen species (ROS) formation, cellular redox status, and enzymes involved in glutathione consumption and regeneration in Aspergillus flavus was investigated. The results demonstrated that CEPA dose dependently inhibited aflatoxin B(1) production. The expression of two typical genes involved in aflatoxin biosynthesis, aflR and aflD, was reduced after CEPA treatment at 7 d. Meanwhile, CEPA significantly reduced ROS production and thiobarbituric acid reactive substances (TBARS), increased the ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG) at 5, 6 and 7 d. The activities of ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2131480 Wed, 31 Dec 2008 05:00:00 +0100 2131480 http://www.medworm.com/index.php?rid=2131481&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19162357%26dopt%3DAbstract Authors: Wevers E, Moons P, Van Houdt R, Lurquin I, Aertsen A, Michiels CW In this work we investigated the role of quorum sensing and specific quorum-sensing dependent properties in the colonization and spoilage of carrot slices by Serratia plymuthica RVH1, a strain isolated previously from a vegetable washing and cutting machine in an industrial kitchen. Disinfected carrot slices were inoculated by immersion in a bacterial suspension and then placed in a Petri dish with a shallow layer of the same bacterial suspension. Subsequently, visible spoilage of the air-exposed upper side of the slices and the evolution of bacterial numbers and pH of the surrounding suspension were recorded during 19 days. A knockout mutant in the N-acyl-homoserine lactone (AHL) synthase splI was clearly compr... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2131481 Wed, 24 Dec 2008 05:00:00 +0100 2131481 http://www.medworm.com/index.php?rid=2129164&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19157613%26dopt%3DAbstract Authors: Le Marc Y, Valík L, Medveďová A The competitive growth of a starter culture of lactic acid bacteria (Fresco 1010, Chr. Hansen, Hørsholm, Denmark) and Staphylococcus aureus was studied in milk. The lactic bacteria (LAB) were able to induce an early stationary state in S. aureus. The developed model highlights that the growth of S. aureus is inhibited when the LAB have reached a critical density. The model was tested in different conditions of temperature (from 12 degrees to 25 degrees C), for various inoculum sizes of LAB and S. aureus. The results show that the model accurately quantifies the kinetics of S. aureus as a function of the starter culture. PMID: 19157613 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2129164 Wed, 24 Dec 2008 05:00:00 +0100 2129164 http://www.medworm.com/index.php?rid=2129163&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19157614%26dopt%3DAbstract Authors: Leroy S, Lebert I, Chacornac JP, Chavant P, Bernardi T, Talon R S. equorum is often isolated from naturally fermented sausages and from the environment of processing units. The aim of this work was first to characterize the genetic diversity of this species in a single small processing unit manufacturing traditional sausages without the use of starter cultures. One hundred and eighteen S. equorum isolates were collected from meat products and surfaces of this unit. Secondly, the capacity to form biofilm of 57 isolates of S. equorum selected from pulsed-field gel electrophoresis (PFGE) profiles was assessed to determine if this property conferred an advantage for the colonization of surfaces in the processing unit. Characterization of the isolates by PFGE analysis revealed a hi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2129163 Wed, 24 Dec 2008 05:00:00 +0100 2129163 http://www.medworm.com/index.php?rid=2129162&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19157616%26dopt%3DAbstract Authors: Kwon GH, Lee HA, Park JY, Kim JS, Lim J, Park CS, Kwon DY, Kim YS, Kim JH A RAPD-PCR (Randomly Amplified Polymorphic DNA-PCR) method was developed for rapid identification of Bacillus species, especially B. subtilis, B. licheniformis, and B. amyloliquefaciens, the most frequently isolated organisms from fermented soy foods such as Cheonggukjang, a Korean traditional food. A RAPD-PCR using a 10-mer (S-30) produced species specific bands reproducibly. All B. subtilis strains tested produced common bands of 0.5 and 0.88 kb in size. All B. amyloliquefaciens strains generated 1.1 and 1.5 kb bands together with 0.5 kb fragment whereas B. licheniformis strains produced 1.25, 1.70, and 1.9 kb bands with an occasional 0.5 kb band. Using the RAPD-PCR protocol, six bacilli strains isolat... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2129162 Wed, 24 Dec 2008 05:00:00 +0100 2129162 http://www.medworm.com/index.php?rid=2129165&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19157611%26dopt%3DAbstract Authors: Luxananil P, Promchai R, Wanasen S, Kamdee S, Thepkasikul P, Plengvidhya V, Visessanguan W, Valyasevi R The use of Lactobacillus plantarum BCC 9546 (LpBCC9546) as a starter culture for Nham, a traditional Thai fermented pork sausage ensures product quality and consistency. However, no direct evidence has confirmed the growth of this starter during Nham fermentation. In order to investigate its role during Nham fermentation, LpBCC9546 was genetically modified to distinguish it from the natural microflora in Nham. LpBCC9546 was transformed with a recombinant plasmid pRV85 to produce the recombinant strain LpG11, which is resistant to erythromycin and emits green fluorescence. LpG11 was used as a starter culture for Nham fermentation, and its growth was monitored by plating on a ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2129165 Tue, 16 Dec 2008 05:00:00 +0100 2129165 http://www.medworm.com/index.php?rid=2110783&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19144432%26dopt%3DAbstract Authors: de Boer E, Zwartkruis-Nahuis JT, Wit B, Huijsdens XW, de Neeling AJ, Bosch T, van Oosterom RA, Vila A, Heuvelink AE Recently the isolation of methicillin-resistant Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade. Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller-Hinton broth+6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, f... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2110783 Sat, 13 Dec 2008 05:00:00 +0100 2110783 http://www.medworm.com/index.php?rid=2104723&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19138809%26dopt%3DAbstract Authors: Zhao W, Yang R, Wang M In this work microbiological shelf life of green tea infusions processed by pulsed electric fields (PEF) treatment (38.4 kV/cm for 200 mus) were assessed at the three storage temperatures of 4, 25 and 37 degrees C, respectively. Immediately after PEF treatment, no viable bacterial cells were detected. However, significant recovery was observed during storage at 25 and 37 degrees C. The total aerobic microbial population increased rapidly in PEF-treated green tea infusions when stored at 25 and 37 degrees C for 14 and 7 days respectively. However, the microbial population remained under 1 log(10) CFU/ml when stored at 4 degrees C up to 180 days. These results demonstrated that a certain proportion of microbial cells in green tea infusions were sublethally... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2104723 Wed, 10 Dec 2008 05:00:00 +0100 2104723 http://www.medworm.com/index.php?rid=2101331&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19136176%26dopt%3DAbstract Authors: Nauta M, Hill A, Rosenquist H, Brynestad S, Fetsch A, van der Logt P, Fazil A, Christensen B, Katsma E, Borck B, Havelaar A In recent years, several quantitative risk assessments for Campylobacter in broiler meat have been developed to support risk managers in controlling this pathogen. The models encompass some or all of the consecutive stages in the broiler meat production chain: primary production, industrial processing, consumer food preparation, and the dose-response relationship. The modelling approaches vary between the models, and this has supported the progress of risk assessment as a research discipline. The risk assessments are not only used to assess the human incidence of campylobacteriosis due to contaminated broiler meat, but more importantly for analyses of the... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2101331 Sun, 07 Dec 2008 05:00:00 +0100 2101331 http://www.medworm.com/index.php?rid=2094956&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19131137%26dopt%3DAbstract In conclusion, direct-PCR after sample enrichment in APW broth was the most successful method for detection of V. parahaemolyticus with the A/P procedure and enumeration by MPN. Better detection was obtained with MPN than with the A/P protocol. Conversely, the plate culture procedure showed better results with the two-step enrichment protocol in which CHROMagar Vibrio was used as the selective agar. PMID: 19131137 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2094956 Sat, 06 Dec 2008 05:00:00 +0100 2094956 http://www.medworm.com/index.php?rid=2092718&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19128851%26dopt%3DAbstract Authors: Zotta T, Asterinou K, Rossano R, Ricciardi A, Varcamonti M, Parente E Streptococcus thermophilus is an important dairy starter used for the manufacture of fermented milks and cheeses. As for many other lactic acid bacteria, its survival during propagation and preservation of starter cultures and its performance in dairy fermentations are significantly affected by its ability to cope with many environmental stresses. This, in turn, is dependent on the expression of a several genes, which is often controlled by negative response regulators, and has complex relationships with the control of metabolism. In this paper we describe the effect of growth phase, adaptation and inactivation of genes for stress response regulators (hrcA, ctsR and rr01) on the growth, acid production and s... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2092718 Wed, 03 Dec 2008 05:00:00 +0100 2092718 http://www.medworm.com/index.php?rid=2067846&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19110333%26dopt%3DAbstract Authors: Rodrigues P, Venâncio A, Kozakiewicz Z, Lima N A polyphasic approach consisting of morphological, chemical and molecular characterization was applied to 31 isolates of Aspergillus Section Flavi originating from Portuguese almonds, with the aim of characterizing and identifying aflatoxigenic and non-aflatoxigenic strains. On the basis of morphological characters (mainly colony color on Czapek-Dox agar and conidia morphology), we found two distinct groups among the population under study: 18 isolates (58%) had dark-green colonies and rough conidia, and were classified as Aspergillus parasiticus; the remaining 13 isolates (42%) had yellow-green colonies and smooth to finely rough globose conidia, and were classified as Aspergillus flavus. Chemical characterization involved t... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2067846 Wed, 03 Dec 2008 05:00:00 +0100 2067846 http://www.medworm.com/index.php?rid=2065481&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19103467%26dopt%3DAbstract Authors: Raghunath P, Karunasagar I, Karunasagar I The efficiency of a new enrichment broth containing bile salt, sodium taurocholate (ST broth) in detecting pathogenic Vibrio parahaemolyticus from seafood was compared with the traditional alkaline peptone water (APW). Both the enrichment broths were compared using 58 seafood samples harvested along the southwest coast of India using conventional isolation, colony hybridization following enrichment (CFE) and PCR. V. parahaemolyticus carrying tdh gene were isolated from 6.9% (4/58) and 3.4% (2/58) of the samples after enrichment in ST broth and APW, respectively. Pathogenic V. parahaemolyticus carrying trh gene were isolated from 20.7% (12/58) and 13.8% (8/58) of the samples after enrichment in ST broth and APW respectively. PCR using e... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2065481 Wed, 03 Dec 2008 05:00:00 +0100 2065481 http://www.medworm.com/index.php?rid=2027605&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19059664%26dopt%3DAbstract Authors: Begum M, Hocking AD, Miskelly D The effect of ultraviolet irradiation (254 nm, UVC) on Aspergillus flavus, Aspergillus niger, Penicillium corylophilum and Eurotium rubrum was investigated using three different exposure techniques. Survival was determined for spores suspended in liquid medium after 1, 2 and 3 min UVC exposure at 4644 J/m(2)/min. The same UVC dose was applied to spores on the surface of agar plates for 5, 10, 15, 30, 60 and 120 s. Spores of A. niger were dried onto a membrane filter, then exposed to UVC treatment. In the liquid medium, treatments from 1-3 min significantly (P<0.001) reduce the number of viable spores. On the surface of agar plates, after a 15 s exposure, a 80-99% reduction of viable spores was observed for all species except A. niger, for whi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2027605 Thu, 27 Nov 2008 05:00:00 +0100 2027605 http://www.medworm.com/index.php?rid=2027606&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19058868%26dopt%3DAbstract Authors: Sikić Pogačar M, Rubeša Mihaljević R, Klančnik A, Brumini G, Abram M, Smole Možina S Although campylobacters are relatively fragile and sensitive to environmental stresses, Campylobacter jejuni has evolved mechanisms for survival in diverse environments, both inside and outside the host. Their survival properties and pathogenic potential were assessed after subjecting food and clinical C. jejuni isolates to different stress conditions. After exposure to starvation (5 h and 15 h of nutrient depletion), a temperature shock (3 min at 55 degrees C) or oxidative stress (5 h and 15 h of atmospheric oxygen) we studied the culturability, viability and capability of adhesion, internalization and survival within the in vitro cell culture model using J... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2027606 Mon, 17 Nov 2008 05:00:00 +0100 2027606 http://www.medworm.com/index.php?rid=2027604&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19059666%26dopt%3DAbstract Authors: Gbassi GK, Vandamme T, Ennahar S, Marchioni E Whey proteins were used as a coating material to improve encapsulation of Lactobacillus plantarum strains in calcium alginate beads. L. plantarum 299v, L. plantarum 800 and L. plantarum CIP A159 were used in this study. Inactivation experiments were carried out in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Cross-sections of freeze-dried beads revealed the random distribution of bacteria throughout the alginate network. From an initial count of 10.04+/-0.01 log(10) CFU g(-1) for L. plantarum 299v, 10.12+/-0.04 for L. plantarum CIP A159 and 10.03+/-0.01 for L. plantarum 800, bacteria in coated beads and incubated in SGF (37 degrees C, 60 min) showed a better survival for L. plantarum 299v, L. plantarum CIP A1... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2027604 Mon, 17 Nov 2008 05:00:00 +0100 2027604 http://www.medworm.com/index.php?rid=2027603&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19064299%26dopt%3DAbstract Authors: Lindqvist R, Lindblad M The purpose of this study was to evaluate maturation and storage conditions as a way to increase the safety of non-heat treated fermented sausages. The specific objectives were to investigate the effects of storage time and temperature on the levels of Escherichia coli, Listeria monocytogenes and Yersinia enterocolitica in fermented sausages and in broth, and to validate how well the broth experiments and some published models can predict inactivation in sausage. One strain each of E. coli, L. monocytogenes and Y. enterocolitica with induced acid tolerance was inoculated into sausage batters representing a typical Swedish recipe for cold-smoked sausages. The sausages were fermented at 27 degrees C for 39 or 48 h and then stored at different temperatures... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=2027603 Mon, 17 Nov 2008 05:00:00 +0100 2027603