MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Food Microbiology' source. Fri, 10 Feb 2012 01:34:35 +0100 http://www.medworm.com/index.php?rid=5534331&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119267%26dopt%3DAbstract Food Micro 2010, 22th International ICFMH Symposium, "Microbial Behaviour in the Food Chain" 30(th) August-3(rd) September 2010, Copenhagen, Denmark. Int J Food Microbiol. 2012 Jan 16;152(3):53 Authors: Dalgaard P, Rådström P, Ingmer H, Borch E, Jakobsen M PMID: 22119267 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5534331 Fri, 23 Dec 2011 15:36:13 +0100 5534331 http://www.medworm.com/index.php?rid=5419671&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22056145%26dopt%3DAbstract Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the "Zona Alta del Río Mendoza" region in Argentina. Int J Food Microbiol. 2011 Dec 15;151(3):319-26 Authors: Mercado L, Sturm ME, Rojo MC, Ciklic I, Martínez C, Combina M Abstract The "Zona Alta del Río Mendoza" (ZARM) is the major Malbec grape viticulture region of Argentina. The aim of the present study was to explore Saccharomyces cerevisiae biodiversity in ZARM vineyards. Interdelta PCR and RFLP mtDNA molecular markers were applied to differentiate S. cerevisiae strains. The presence of commercial strains on ZARM vineyards was also assessed. Our results reveal a highly diverse, but genetically closely related, S. cerevisiae population (containing more than 190 molecular patterns among 590 ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5419671 Fri, 18 Nov 2011 15:12:11 +0100 5419671 http://www.medworm.com/index.php?rid=5296953&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872958%26dopt%3DAbstract Authors: Arroyo C, Cebrián G, Pagán R, Condón S Abstract The inactivation of Cronobacter sakazakii by heat and ultrasound treatments under pressure at different temperatures [manosonication (MS) and manothermosonication (MTS)] was studied in citrate-phosphate pH 7.0 buffer and rehydrated powdered milk. The inactivation rate was an exponential function of the treatment time for MS/MTS treatments (35-68°C; 200kPa of pressure; 117μm of amplitude of ultrasonic waves) in both media, and for thermal treatments alone when buffer was used as heating media. Survival curves of C. sakazakii during heating in milk had a concave downward profile. Up to 50°C, the lethality of ultrasound under pressure treatments was independent of the treatment temperature in both media. At temperatures gr... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296953 Sun, 09 Oct 2011 03:09:09 +0100 5296953 http://www.medworm.com/index.php?rid=5296952&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872959%26dopt%3DAbstract Authors: McKellar RC, Delaquis P Abstract Escherichia coli O157:H7, an occasional contaminant of fresh produce, can present a serious health risk in minimally processed leafy green vegetables. A good predictive model is needed for Quantitative Risk Assessment (QRA) purposes, which adequately describes the growth or die-off of this pathogen under variable temperature conditions experienced during processing, storage and shipping. Literature data on behaviour of this pathogen on fresh-cut lettuce and spinach was taken from published graphs by digitization, published tables or from personal communications. A three-phase growth function was fitted to the data from 13 studies, and a square root model for growth rate (μ) as a function of temperature was derived: μ=(0.023*(Temperature-1... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296952 Sun, 09 Oct 2011 03:08:56 +0100 5296952 http://www.medworm.com/index.php?rid=5296951&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872960%26dopt%3DAbstract Authors: Mols M, Ceragioli M, Abee T Abstract Bacillus cereus is a food-borne human pathogen and food spoilage organism. Spores and vegetative cells of B. cereus can be found almost everywhere and therefore often end up in food processing equipment and food products. To remove spores and vegetative cells from food or equipment, harsh treatments such as high temperatures are applied. The heat stress response of B. cereus and other organisms has been studied and it has been shown that reactive oxygen species may be involved in inactivating the bacterial cells. Using a novel approach with the fluorescent probe MitoSOX, the formation of superoxide in B. cereus cells upon exposure to heat has been confirmed. MitoSOX can be used in combination with other probes, including, SYTOX green, C... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296951 Sun, 09 Oct 2011 03:08:43 +0100 5296951 http://www.medworm.com/index.php?rid=5296950&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872961%26dopt%3DAbstract Authors: Methner U, Rammler N, Fehlhaber K, Rösler U Abstract Apart from Salmonella monitoring of pig herds during the period of growth to evaluate the efficacy of control programmes, monitoring at harvest level is of relevance to assess the Salmonella status of fattening pigs and the associated risk of introducing Salmonella organisms in the slaughter process. Samples from 1830 fattening pigs were gathered at slaughter. Ileocaecal lymph nodes, rectal and caecal content as well as tonsils were collected for bacteriological examinations, and a part of the diaphragm pillar muscle was taken to gain meat-juice for serological analysis. Salmonella spp. was recovered from 13.8% of all pigs examined. Salmonella Typhimurium and Derby were the dominating serovars. The highest detection rat... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296950 Sun, 09 Oct 2011 03:08:30 +0100 5296950 http://www.medworm.com/index.php?rid=5296949&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21875757%26dopt%3DAbstract Authors: Almofti YA, Dai M, Sun Y, Hao H, Liu Z, Cheng G, Yuan Z Abstract Physiologic and phenotypic alterations in the context of antibiotic resistance have been extensively studied in some bacteria. However there are not enough data addressing these alterations due to macrolide resistance in Campylobacter jejuni. The present study examined the fitness cost imposed by different macrolide resistance mutations and the phenotypic alterations due to exposure to macrolides in C. jejuni. C. jejuni was induced with different macrolide agents to obtain different macrolide resistance mutations. The results revealed that the mutations significantly imposed defect variations on the doubling time and the relative fitness in the resistant strains when competed against the susceptible strain. F... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296949 Sun, 09 Oct 2011 03:08:18 +0100 5296949 http://www.medworm.com/index.php?rid=5296948&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21878400%26dopt%3DAbstract In conclusion, the qPCR assay developed here represents a valuable tool for rapid detection and quantification of STEC in foods such as minced beef and dairy products as it ensures a high sensitivity and an optimal STEC diagnostic spectrum, taking into account the genetic stx variability observed in STEC population. PMID: 21878400 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296948 Sun, 09 Oct 2011 03:08:06 +0100 5296948 http://www.medworm.com/index.php?rid=5296947&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21880388%26dopt%3DAbstract Authors: Saldaña G, Puértolas E, Monfort S, Raso J, Alvarez I Abstract The influence of temperature and the presence of N(α)-lauroyl ethylester (ethyl lauroyl arginate, LAE) on the inactivation caused by continuous pulsed electric field treatments (PEF) in Escherichia coli O157:H7 suspended in apple juice have been investigated to define treatment conditions applicable at industrial scale that promote an equivalent safety level when compared with thermal processing. In the range of experimental conditions investigated (outlet temperature: 20-40°C, electric field strength: 20-30kV, treatment time: 5-125μs) at outlet temperatures equal or lower than 55±1°C, the inactivation of E. coli O157:H7 treated in apple juice ranged from 0.4 to 3.6 Log(10) cycles reduction and treated in... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296947 Sun, 09 Oct 2011 03:07:54 +0100 5296947 http://www.medworm.com/index.php?rid=5296946&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21893360%26dopt%3DAbstract The objective was to establish the potential of these technologies, applied individually or in paired sequences, to inactivate Escherichia coli and Pichia fermentans inoculated in a fresh blend of apple and cranberry juice. The shelf-life evaluation of selected non-thermally treated samples was conducted over 35days and compared to pasteurised samples and untreated juices. All treatments applied individually significantly reduced (1.8-6.0logcfu/ml) microbial counts compared to the untreated sample (p<0.01). Furthermore, UV treatment produced significantly greater inactivation (p<0.05) for E. coli compared to P. fermentans. Combinations of non-thermal hurdles consisting of UV or HILP followed by either PEF or MTS resulted in comparable reductions for both microorganisms (p≥0.05) to ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296946 Sun, 09 Oct 2011 03:07:16 +0100 5296946 http://www.medworm.com/index.php?rid=5296945&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21893361%26dopt%3DAbstract This study investigated the spatial distribution of Cronobacter spp. in powdered infant formula (PIF) on industrial batch-scale for both a recalled batch as well a reference batch. Additionally, local spatial occurrence of clusters of Cronobacter cells was assessed, as well as the performance of typical sampling strategies to determine the presence of the microorganisms. The concentration of Cronobacter spp. was assessed in the course of the filling time of each batch, by taking samples of 333g using the most probable number (MPN) enrichment technique. The occurrence of clusters of Cronobacter spp. cells was investigated by plate counting. From the recalled batch, 415 MPN samples were drawn. The expected heterogeneous distribution of Cronobacter spp. could be quantified from these samples,... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296945 Sun, 09 Oct 2011 03:07:04 +0100 5296945 http://www.medworm.com/index.php?rid=5296944&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21924791%26dopt%3DAbstract This study illustrates the necessity of determining the effectiveness of technological processes against enteric viruses, using a relevant reference such as HAV, in order to reduce the risk of hepatitis and gastroenteritis by exposure to vegetables. PMID: 21924791 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296944 Sun, 09 Oct 2011 03:05:56 +0100 5296944 http://www.medworm.com/index.php?rid=5296942&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21974979%26dopt%3DAbstract Authors: Navarro D, Díaz-Mula HM, Guillén F, Zapata PJ, Castillo S, Serrano M, Valero D, Martínez-Romero D Abstract Two nectarine cultivars ('Flavela' and 'Flanoba') were treated with Aloe vera gel alone, or with the addition of thymol, and then inoculated with Rhizopus stolonifer, Botrytis cinerea and Penicillium digitatum. Both treatments were effective in reducing the decay incidence caused by the 3 fungi species, although the addition of thymol did not generally improve the efficacy of Aloe vera gel on reducing the infection damage. The coatings were clearly effective in reducing the postharvest ripening process of both nectarine cultivars manifested by a delay in ethylene production and respiration rate, weight loss and softening. Interestingly, these coatings showed effect... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296942 Sat, 17 Sep 2011 04:00:00 +0100 5296942 http://www.medworm.com/index.php?rid=5296941&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21974980%26dopt%3DAbstract Authors: Angelidis AS, Tirodimos I, Bobos M, Kalamaki MS, Papageorgiou DK, Arvanitidou M Abstract The transmission pathways of Helicobacter pylori in humans have not been fully elucidated. Research in the last decade has proposed that foodborne transmission, among others, may be a plausible route of human infection. Owing to the organism's fastidious growth characteristics and its ability to convert to viable, yet unculturable states upon exposure to stress conditions, the detection of H. pylori in foods via culture-dependent methods has been proven to be laborious, difficult and in most cases unsuccessful. Hence, nucleic acid-based methods have been proposed as alternative methods but, to date, only PCR-based methods have been reported in the literature. In the current study, fluo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296941 Sat, 17 Sep 2011 04:00:00 +0100 5296941 http://www.medworm.com/index.php?rid=5296940&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21978657%26dopt%3DAbstract Authors: Santos CA, Almeida FS, Guimarães AG, Abrahão WM, Arantes OM, Vilas-Bôas GT Abstract Twenty-three Bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from Brazilian soil samples were analyzed for the presence and genetic diversity (by RE-PCR) of the virulence genes ces (emetic toxin, cereulide), plcR-papR (pleiotropic regulator PlcR and peptide PapR), nheA (a component of the NHE complex), bceT (diarrheal enterotoxin bc-D-ENT), gyrB (B subunit of DNA gyrase), cytK-2 (necrotic enterotoxin cytotoxin K-2), and plcA (phosphatidylcholine-specific phospholipase C). Additionally, these isolates were phenotypically characterized for motility, hemolyti... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296940 Sat, 17 Sep 2011 04:00:00 +0100 5296940 http://www.medworm.com/index.php?rid=5296955&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21962939%26dopt%3DAbstract In conclusion we have shown that H. vineae inoculated as part of a sequential mixed starter is able to compete with native yeasts present in non-sterile must and modify the wine aroma profile. PMID: 21962939 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296955 Wed, 14 Sep 2011 04:00:00 +0100 5296955 http://www.medworm.com/index.php?rid=5296943&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21968113%26dopt%3DAbstract Authors: Szlavik J, Paiva DS, Mørk N, van den Berg F, Verran J, Whitehead K, Knøchel S, Nielsen DS Abstract Some strains of the food borne pathogen Listeria monocytogenes persist in food processing environments. The exact reason behind this phenomenon is not known, but strain differences in the ability to adhere to solid surfaces could offer an explanation. In the present work, initial adhesion of nine strains of L. monocytogenes was investigated under liquid flow at two levels of shear stress on six different surfaces using a flow chamber set-up with microscopy measurements. The surfaces tested were glass and PVC, and glass coated with beef extract, casein, and homogenised and unhomogenised milk. In addition, the effect of prior environmental stress (5% NaCl, low nutrient availa... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296943 Wed, 14 Sep 2011 04:00:00 +0100 5296943 http://www.medworm.com/index.php?rid=5296954&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21962940%26dopt%3DAbstract Authors: Oteiza JM, Ares G, Sant'ana AS, Soto S, Giannuzzi L Abstract The purpose of this study was to determine the incidence of Alicyclobacillus spp. in fruit/vegetable juices (concentrated pulps and clarified and non-clarified juices) marketed in Argentina between 1996 and 2009. The presence of Alicyclobacillus was determined in a total of 8556 samples of fruit and vegetable juices (apple, pear, grape, peach, blend of juices, tangerine, pineapple, orange, mango, plum, guava, apricot, lemon, banana, kiwi, carrot, strawberry, grapefruit, and beetroot) collected in seven Argentinean provinces. Multiple factor analysis (MFA) was carried out on a data matrix that contained the percentage of positive samples, type of juice, raw material and production year. Except for kiwi and orange,... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5296954 Sat, 10 Sep 2011 04:00:00 +0100 5296954 http://www.medworm.com/index.php?rid=5279763&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944662%26dopt%3DAbstract Authors: Mhone TA, Matope G, Saidi PT Abstract A cross sectional study was conducted to enumerate total viable bacteria (TBC), coliforms, Escherichia coli and Staphylococcus aureus in raw (n=120) and processed (n=20) milk from individual farms from three smallholder dairy schemes of Zimbabwe between October, 2009 and February, 2010. Data on management factors were collected using a structured questionnaire. A standard pour plate technique was used to enumerate total viable bacteria, while for coliforms, E. coli and S. aureus, counts were assessed by the spread plate technique. The association of total viable bacterial counts and management factors was assessed using univariable and a linear regression model. The log(10) TBC for raw milk differed significantly (P<0.05) amongst th... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5279763 Sat, 10 Sep 2011 04:00:00 +0100 5279763 http://www.medworm.com/index.php?rid=5279762&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944758%26dopt%3DAbstract In this study, we applied the denaturing high-performance liquid chromatography (D-HPLC) technique, combined with the amplification of the ITS1 region from fungal rDNA, for the rapid identification of major fungal species in 9 raw milk samples from cow, ewe and goat, collected at different periods of the year. A total of 27 fungal species were identified. Yeast species belonged to Candida, Cryptococcus, Debaryomyces, Geotrichum, Kluyveromyces, Malassezia, Pichia, Rhodotorula and Trichosporon genera; and mold species belonged to Aspergillus, Chrysosporium, Cladosporium, Engyodontium, Fusarium, Penicillium and Torrubiella genera. Cow milk samples harbored the highest fungal diversity with a maximum of 15 species in a single sample, whereas a maximum of 4 and 6 different species were recovere... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5279762 Thu, 08 Sep 2011 04:00:00 +0100 5279762 http://www.medworm.com/index.php?rid=5279761&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21955732%26dopt%3DAbstract Authors: Barbuddhe SB, Malik SV, Kumar JA, Kalorey DR, Chakraborty T Abstract Listeria monocytogenes is a foodborne pathogen that can cause serious invasive illness, mainly in certain well-defined high-risk groups, including elderly and immunocompromised patients, pregnant women, newborns and infants. In India, this pathogen has been isolated from humans, animals and foods. The incidence of Listeria is generally comparable to those reported elsewhere in the world. In humans, maternal/neonatal listeriosis is the most common clinical form reported. Among animal populations, spontaneous abortions, subclinical mastitis, meningoencephalitis and endometritis were the commonest forms reported. The disease largely remains undiagnosed and under reported. From reported analyses of a variety ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5279761 Thu, 08 Sep 2011 04:00:00 +0100 5279761 http://www.medworm.com/index.php?rid=5246561&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21924789%26dopt%3DAbstract In this study, the ability of low populations of E. coli to survive during production and postharvest operations in selected mini-greens has been demonstrated. However, further field-based trials are needed to expand understanding of the post-contamination fate of enteric bacterial pathogens on leafy vegetables. In summary, this research work provides baseline data upon which to develop food safety preventive control guidance during the production and minimal processing of these crops. PMID: 21924789 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5246561 Fri, 02 Sep 2011 04:00:00 +0100 5246561 http://www.medworm.com/index.php?rid=5246560&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21925757%26dopt%3DAbstract Authors: Fernández-No IC, Böhme K, Calo-Mata P, Barros-Velázquez J Abstract Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were preliminarily selected on Niven medium. Two of these isolates were confirmed as prolific histamine producers by HPLC. Thus, Pseudomonas fragi (isolated from turbot) and Pseudomonas syringae (isolated from blackspo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5246560 Fri, 02 Sep 2011 04:00:00 +0100 5246560 http://www.medworm.com/index.php?rid=5246562&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21924512%26dopt%3DAbstract Authors: Levy C, Aubert X, Lacour B, Carlin F Abstract Pulsed Light (PL) uses intense flashes of white light rich in ultraviolet (UV) light for decontamination. A log-reduction higher than 5 was obtained in one flash and at fluences lower than 1.8J/cm(2) on spores of a range of spore-forming bacteria, of vegetative cells of non-spore-forming bacteria and on yeasts spread on agar media. Vegetative cells were more sensitive than spores. The inactivation by PL of Bacillus subtilis, B. atrophaeus, B. cereus, Geobacillus stearothermophilus, and Aspergillus niger spores sprayed on polystyrene was similar. The inactivation by PL of B. subtilis and A. niger spores sprayed on glass was slightly lower than on polystyrene. No alteration of the spore structures was detected by scanning electro... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5246562 Wed, 31 Aug 2011 04:00:00 +0100 5246562 http://www.medworm.com/index.php?rid=5246559&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21925758%26dopt%3DAbstract Authors: Garcia D, Ramos AJ, Sanchis V, Marín S Abstract Penicillium expansum is the main species responsible for patulin production in apples and pears. Generally, fruit is stored at suboptimal conditions for mould growth and this situation could influence on the intra-species variability in both capability for growth and mycotoxin production. The aim of this research was to assess the impact of suboptimal environmental conditions on the intra-specific variability of P. expansum growth and patulin production using seventy nine isolates of this mould. Petri dishes with Apple Concentrate Agar Medium (ACAM) were inoculated centrally and incubated at two temperatures, one near optimal (20°C) and the other representative of suboptimal cold storage (1°C). For each condition, 10 Petri... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5246559 Wed, 31 Aug 2011 04:00:00 +0100 5246559 http://www.medworm.com/index.php?rid=5229916&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21920618%26dopt%3DAbstract Authors: Geng P, Chen S, Hu M, Rizwan-Ul-Haq M, Lai K, Qu F, Zhang Y Abstract Biocontrol efficacy of an antagonistic yeast Kluyveromyces marxianus was evaluated individually or in combination with sodium bicarbonate (SBC) against green mold of citrus fruit caused by Penicillium digitatum. Their effects on postharvest quality of citrus fruit were also investigated. The results indicated that the antagonistic activity of K. marxianus at 1×10(8)CFU/mL on green mold of citrus fruit was enhanced by 2% SBC treatment. In artificial inoculation trials, disease control after 3 and 6days, respectively, with the mixture of K. marxianus and 2% SBC (18.33%, 58.33%) was significantly improved over that obtained with K. marxianus (41.67%, 70.00%) or SBC (43.33%, 81.67%) alone. The combination of... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5229916 Wed, 31 Aug 2011 04:00:00 +0100 5229916 http://www.medworm.com/index.php?rid=5229918&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21917347%26dopt%3DAbstract Authors: Hauser E, Hebner F, Tietze E, Helmuth R, Junker E, Prager R, Schroeter A, Rabsch W, Fruth A, Malorny B Abstract Salmonella enterica serovar Derby (S. Derby) is one of the most prevalent serovars in pigs in Europe and in the U.S. and ranks among the 10 most frequently isolated serovars in humans. Therefore, a set of 82 epidemiologically unrelated S. Derby strains isolated between 2006 and 2008 from pigs, pork and humans in Germany was selected and investigated in respect to the transmission of clonal groups of the serovar along the food chain. Various phenotypic and genotypic methods were applied and the pathogenicity and resistance gene repertoire was determined. Phenotypically 72% of the strains were susceptible to all 17 antimicrobials tested while the others were monore... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5229918 Sat, 27 Aug 2011 04:00:00 +0100 5229918 http://www.medworm.com/index.php?rid=5229919&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21914558%26dopt%3DAbstract In this study the microbiota of Atlantic mackerel (Scomber scombrus) collected by a commercial purse seiner was examined. Fish were collected directly from the purse seine and from the Refrigerated Sea Water (RSW) transport tank after loading. The culturable microbiota and Specific Spoilage Bacteria (SSB) were quantified on Iron Agar Lyngby (IAL) and identified using commercially available Biochemical API® kits on pure cultured isolates. These kits showed to be sub-optimal in characterising the isolates, since only half of the strains were identified. The same isolates were also identified by a nucleic acid based PCR-DGGE approach, and only half of the sequences gave the same results as the API®. Characterisation by PCR-DGGE was also performed on bacterial DNA from IAL plates (bulk cell ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5229919 Fri, 26 Aug 2011 04:00:00 +0100 5229919 http://www.medworm.com/index.php?rid=5229917&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21917348%26dopt%3DAbstract Authors: Straube J, Albert T, Manteufel J, Heinze J, Fehlhaber K, Truyen U Abstract The importance of foodborne viruses is increasingly recognized. Thus, the effect of commonly used food preservation methods on the infectivity of viruses is questioned. In this context, we investigated the antiviral properties of d,l-lactic acid, sodium chloride and sodium nitrite by in vitro studies. Two model viruses, Feline Calicivirus (FCV) and Enteric Cytophatic Human Orphan (ECHO) virus, were chosen for this study simulating important foodborne viruses (human noroviruses (NoV) and human enteroviruses, resp.). The model viruses were exposed to different solutions of d,l-lactic acid (0.1-0.4% w/w, pH 6.0-3.2), of sodium chloride (2-20%, w/v) and of sodium nitrite (100, 150 and 200ppm) at 4 and 2... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5229917 Wed, 24 Aug 2011 04:00:00 +0100 5229917 http://www.medworm.com/index.php?rid=5218889&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899907%26dopt%3DAbstract Authors: Hooton SP, Atterbury RJ, Connerton IF Abstract Multidrug-resistant Salmonella Typhimurium U288 is a significant pathogen of pigs, accounting for over half of all outbreaks on UK pig production premises. The potential of this serovar, and other salmonellae, to enter the food chain during the slaughtering process requires that efforts be made to reduce the prevalence of these bacteria at both the pre- and post-harvest stages of production. A bacteriophage cocktail (PC1) capable of lysing various Salmonella enterica serovars was designed using the broad host-range phage Felix 01, and three phages isolated from sewage. PC1 applied to pig skin experimentally-contaminated with U288 achieved significant reductions (P<0.05) in Salmonella counts when stored at 4°C over 96h. Red... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218889 Mon, 22 Aug 2011 04:00:00 +0100 5218889 http://www.medworm.com/index.php?rid=5218888&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899908%26dopt%3DAbstract Authors: Gil-Serna J, Patiño B, Cortés L, González-Jaén MT, Vázquez C Abstract Aspergillus westerdijkiae is one of the most relevant ochratoxin A (OTA) producing species within the Section Circumdati contaminating a number of agroproducts. The yeast Debaryomyces hansenii CYC 1244 was previously reported to be able to reduce growth and extracellular OTA produced by A. westerdijkiae. In this work, we examined several mechanisms possibly involved in this OTA reduction in in vitro experiments. OTA biosynthesis was evaluated by quantitation of expression levels of pks (polyketide synthase) and p450-B03 (cytochrome p450 monooxygenase) genes using newly developed and specific real time RT-PCR protocols. Both genes showed significant lower levels in presence of D. hansenii CYC 1244 su... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218888 Mon, 22 Aug 2011 04:00:00 +0100 5218888 http://www.medworm.com/index.php?rid=5218886&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21903288%26dopt%3DAbstract Authors: Yang Y, Bouras N, Yang J, Howard RJ, Strelkov SE Abstract Internal fruit rot, caused by Fusarium lactis, is an important disease of sweet pepper (Capsicum annuum) in Canadian greenhouses. Production of the mycotoxins fumonisin B(1) (FB(1)), moniliformin (MON) and beauvericin (BEA) by F. lactis (17 isolates) and the related species F. proliferatum (three isolates) and F. verticillioides (one isolate), which are also associated with internal fruit rot, was evaluated on rice medium. All 21 isolates examined were found to produce BEA, at concentrations ranging from 13.28 to 1674.60ppm, while 13 of 17 F. lactis isolates and two of three F. proliferatum isolates produced MON (0.23 to 181.85ppm). Only one isolate of F. lactis produced detectable levels of FB(1) in culture, wherea... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218886 Mon, 22 Aug 2011 04:00:00 +0100 5218886 http://www.medworm.com/index.php?rid=5218885&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21903289%26dopt%3DAbstract Authors: Valera MJ, Laich F, González SS, Torija MJ, Mateo E, Mas A Abstract The identification of acetic acid bacteria (AAB) from sound grapes from the Canary Islands is reported in the present study. No direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from Tenerife, La Palma and Lanzarote islands. Up to 396 AAB were isolated from those microvinifications and identified by 16S rRNA gene sequencing and phylogenetic analysis. With this method, Acetobacter pasteurianus, Acetobacter tropicalis, Gluconobacter japonicus and Gluconacetobacter saccharivorans were identified. However, no discrimination between the closely related species Acetobacter malorum and Acetobacter cerevisiae was possible. As previously descr... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218885 Mon, 22 Aug 2011 04:00:00 +0100 5218885 http://www.medworm.com/index.php?rid=5218884&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21903290%26dopt%3DAbstract Authors: Morán-Marroquín GA, Córdova J, Valle-Rodríguez JO, Estarrón-Espinosa M, Díaz-Montaño DM Abstract Knowledge of physiological behavior of indigenous tequila yeast used in fermentation process is still limited. Yeasts have significant impact on the productivity fermentation process as well as the sensorial characteristics of the alcoholic beverage. For these reasons a better knowledge of the physiological and metabolic features of these yeasts is required. The effects of dilution rate, nitrogen and phosphorus source addition and micro-aeration on growth, fermentation and synthesis of volatile compounds of two native Saccharomyces cerevisiae strains, cultured in continuous fed with Agave tequilana juice were studied. For S1 and S2 strains, maximal concentrations of biom... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218884 Mon, 22 Aug 2011 04:00:00 +0100 5218884 http://www.medworm.com/index.php?rid=5218891&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21889226%26dopt%3DAbstract Authors: Mugrabi de Kuppler AL, Steiner U, Sulyok M, Krska R, Oerke EC Abstract Fusarium graminearum is the most important pathogen causing Fusarium head blight (FHB) of small cereal grains worldwide responsible for quantitative and qualitative yield losses. The presence in crops is often associated with mycotoxin contamination of foodstuff limiting its use for human and animal consumption. A collection of isolates of F. graminearum from Germany was characterized genetically and chemically for their potential to produce the B trichothecenes deoxynivalenol (DON) and nivalenol (NIV). Molecular methods with eight PCR assays were implemented based on functional Tri7 and Tri13 genes and on the tri5-tri6 intergenic region to differentiate between chemotaxonomic groups DON and NIV, result... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218891 Wed, 17 Aug 2011 04:00:00 +0100 5218891 http://www.medworm.com/index.php?rid=5218890&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21893359%26dopt%3DAbstract Authors: Ponsone ML, Chiotta ML, Combina M, Dalcero A, Chulze S Abstract The efficacy of two strains of Kluyveromyces thermotolerans in preventing the growth and ochratoxin A (OTA) accumulation of ochratoxigenic fungi both "in vitro" and "in situ" was evaluated. The data from this study showed that both yeast strains were able to control Aspergillus carbonarius and A. niger aggregate species growth and ochratoxin A accumulation. The inhibitory effects were dependent on the ochratoxigenic species, yeast strains, a(w) and temperature evaluated and their interactions. Over all conditions assayed, ochratoxin A accumulation was reduced from 3% to 100% and the growth rate from 11% to 82.5%, depending on conditions. These results are promising for future development of a bio-pesticide. ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218890 Wed, 17 Aug 2011 04:00:00 +0100 5218890 http://www.medworm.com/index.php?rid=5218887&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899909%26dopt%3DAbstract Authors: Li H, Liu C, Chen L, Zhang X, Cai J Abstract Bdellovibrio-and-like organisms (BALOs) are a group of highly motile delta-proteobacteria that prey on other gram-negative bacteria. However, nothing is known of the application potential of marine BALOs in safeguarding seafood safety. Here, biological characterization of two marine BALOs strains and their application in the elimination of Vibrio parahaemolyticus in oyster (Crassostrea ariakensis) at the laboratory scale were investigated. BALOs strains BDH12 and BDHSH06 were isolated from sediment of Daya bay in Shenzhen of China, with Shewanella putrefaciens strain 12 and V. parahaemolyticus strain SH06 as preys, respectively, when using double layer agar technique. They were identified as BALOs morphologically by transmission... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5218887 Tue, 16 Aug 2011 04:00:00 +0100 5218887 http://www.medworm.com/index.php?rid=5006586&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21700353%26dopt%3DAbstract Authors: Schwaiger K, Helmke K, Hölzel CS, Bauer J The aim of this study was to elucidate whether and to what extent fresh produce from Germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. For this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in Germany and examined bacteriologically. Phenotypic resistance of Enterobacter cloacae (n=172); Enterobacter gergoviae (n=92); Pantoea agglomerans (n=96); Pseudomonas aeruginosa (n=295); Pseudomonas putida (n=106) and Enterococcus faecalis (n=100) against up to 30 antibiotics was determined by using the microdilution method. Resistance to ß-lactams was most frequently expressed by P. agglomerans and E. gergoviae against cefaclor (41%... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=5006586 Fri, 08 Jul 2011 11:30:54 +0100 5006586 http://www.medworm.com/index.php?rid=4911960&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21616548%26dopt%3DAbstract Authors: Satomi M, Furushita M, Oikawa H, Yano Y Nineteen isolates of histamine producing halophilic bacteria were isolated from four fish sauce mashes, each mash accumulating over 1000ppm of histamine. The complete sequences of the plasmids encoding the pyruvoyl dependent histidine decarboxylase gene (hdcA), which is harbored in histamine producing bacteria, were determined. In conjunction, the sequence regions adjacent to hdcA were analyzed to provide information regarding its genetic origin. As reference strains, Tetragenococcus halophilus H and T. muriaticus JCM10006(T) were also studied. Phenotypic and 16S rRNA gene sequence analyses identified all isolates as T. halophilus, a predominant histamine producing bacteria present during fish sauce fermentation. Genetic analyses (PCR, S... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4911960 Thu, 09 Jun 2011 07:00:21 +0100 4911960 http://www.medworm.com/index.php?rid=4911959&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21620506%26dopt%3DAbstract Authors: Cordero-Bueso G, Arroyo T, Serrano A, Valero E Some oenological practices, such as the massive utilisation of commercial yeast and the consequent colonisation of wineries, can contribute to reducing the native yeast biodiversity. In this context, the vineyard could be a reservoir of autochthonous yeasts of oenological interest. Thus, the evaluation of the influence of different agricultural parameters on the biodiversity of yeast population in the vineyard is necessary. This work shows the results of the influence of some floor management strategies of the vineyard in the natural yeast population associated with the grape-berries. With this objective, a three year sampling plan was designed in the Shiraz vineyards of the Madrid region using three floor management strategies: b... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4911959 Thu, 09 Jun 2011 07:00:16 +0100 4911959 http://www.medworm.com/index.php?rid=4911958&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21620507%26dopt%3DAbstract Authors: Bernardi C, Gustinelli A, Fioravanti ML, Caffara M, Mattiucci S, Cattaneo P Viscera and muscle of a total of 40 wild 1-2kg European sea bass (Dicentrarchus labrax) from Northeast Atlantic (FAO area 27) were examined for Anisakidae larvae detection by digestion method. Extracted parasites were counted and mean intensity was calculated. Parasites were identified by genetic/molecular markers (allozymes and sequences analysis of the mtDNA cox2 gene) as belonging to the species Anisakis simplex (sensu stricto). In viscera, the main localisations of the larvae were under the gastric serosa, where several parasites alive and dead were found, and intestinal serosa. The visceral prevalence was 0.950 and the mean intensity was 96.39. The main localisation of A. simplex (s.s.) in edible ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4911958 Thu, 09 Jun 2011 07:00:12 +0100 4911958 http://www.medworm.com/index.php?rid=4862390&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21601302%26dopt%3DAbstract Authors: Hsouna AB, Trigui M, Mansour RB, Jarraya RM, Damak M, Jaoua S The present study describes the phytochemical profile and the protective effects of Ceratonia siliqua pods essential oil (CsEO), a food and medicinal plant widely distributed in Tunisia. Twenty five different components were identified in the CsEO. Among them, the major detected components were: Nonadecane, Heneicosane , Naphthalene, 1,2-Benzenedicarboxylic acid dibutylester, Heptadecane, Hexadecanoic acid, Octadecanoic acid, 1,2-Benzenedicarboxylic acid, Phenyl ethyl tiglate, Eicosene, Farnesol 3, Camphor, Nerolidol and n-Eicosane. The antimicrobial activity of CsEO was evaluated against a panel of 13 bacteria and 8 fungal strains using agar diffusion and broth microdilution methods. Results have shown that CsEO ex... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4862390 Wed, 18 May 2011 23:00:00 +0100 4862390 http://www.medworm.com/index.php?rid=4805027&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21440319%26dopt%3DAbstract In this study we analyzed 97 foodborne B. cereus sensu stricto strains isolated in Brazil in the 1980's, 1990's and 2000's in order to investigate the genetic diversity (assessed by Rep-PCR), antimicrobial resistance and toxigenic profiles (presence of hblA, hblC and hblD; nheA, nheB and nheC as well as cytK, ces and entFM genes) of such strains. The majority of the strains (79, 81.4%) were β-hemolytic. The NHE complex was found in 82 strains (84.5%) and HBL complex was found in 61 (62.9%) strains. All strains were negative to ces. The cytK-2 gene was found in 44 (45.4%) strains. The predominant toxigenic pattern was type I (32, 33%) which included strains positive for all toxin genes but ces. Computer assisted cluster analysis of Rep-PCR profiles showed a high genetic diversity. Seven ma... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805027 Wed, 11 May 2011 12:33:48 +0100 4805027 http://www.medworm.com/index.php?rid=4805026&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21440945%26dopt%3DAbstract This study suggests that the development of FAHA species in early ripening likely contributes to the initial part of the in situ inhibition exerted by the complex cheese surface ecosystem investigated. PMID: 21440945 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805026 Wed, 11 May 2011 12:33:44 +0100 4805026 http://www.medworm.com/index.php?rid=4805025&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21440946%26dopt%3DAbstract Authors: Huang L PMID: 21440946 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805025 Wed, 11 May 2011 12:33:41 +0100 4805025 http://www.medworm.com/index.php?rid=4805024&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21444120%26dopt%3DAbstract Authors: Alborch L, Bragulat MR, Abarca ML, Cabañes FJ The aim of this study was to determine the effects of water activity (a(w)) (0.92-0.98), temperature (5-45°C) and incubation time (5-60days) on growth and ochratoxin A (OTA) production by Aspergillus niger and Aspergillus carbonarius on maize kernels using a simple method. Colony diameters of both strains at 0.92 a(w) were significantly lower than those at 0.96 and 0.98 a(w) levels. The optimum growth temperature range for A. niger was 25-40°C and for A. carbonarius 20-35°C. A. niger produced OTA from 15 to 40°C, and the highest OTA level was recorded at 15°C. The concentration of OTA produced at 0.92 a(w) was significantly lower than those at 0.96 and 0.98 a(w). A. carbonarius produced OTA from 15 to 35°C and the maximum co... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805024 Wed, 11 May 2011 12:33:37 +0100 4805024 http://www.medworm.com/index.php?rid=4805023&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450361%26dopt%3DAbstract Authors: Lutterschmid G, Muranyi M, Stübner M, Vogel RF, Niessen L The spontaneous over-foaming of beer upon opening, i.e. beer gushing, is an unwanted phenomenon for the brewing industry. Currently, surface-active proteins from filamentous fungi and non-specific lipid transfer proteins (nsLTP1) from barley are discussed as gushing inducers. In our study the class I hydrophobin FcHyd3p from Fusarium culmorum, the class II hydrophobin Hfb2 from Trichoderma reesei, the alkaline foam protein A (AfpA) from F. graminearum and nsLTP1 from Hordeum vulgare cv. Marnie (barley) were heterologously expressed in Pichia pastoris and used in gushing tests. The class I hydrophobin FcHyd3p was unable to induce gushing in beer. The class II hydrophobin Hfb2 was able to induce gushing in beer, but prov... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805023 Wed, 11 May 2011 12:33:33 +0100 4805023 http://www.medworm.com/index.php?rid=4805021&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21458095%26dopt%3DAbstract Authors: Servili M, Rizzello CG, Taticchi A, Esposto S, Urbani S, Mazzacane F, Di Maio I, Selvaggini R, Gobbetti M, Di Cagno R Functional milk beverages (FMB100 and FMB200) fortified with phenolic compounds (100 and 200mg/l) extracted from olive vegetable water, and fermented with γ-amino butyric acid (GABA)-producing (Lactobacillus plantarum C48) and autochthonous human gastro-intestinal (Lactobacillus paracasei 15N) lactic acid bacteria were manufactured. A milk beverage (MB), without addition of phenolic compounds, was used as the control. Except for a longer latency phase of FMB200, the three beverages showed an almost similar kinetic of acidification, consumption of lactose and synthesis of lactic acid. Apart from the beverage, Lb. plantarum C48 showed a decrease of ca. Log 2.52-... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805021 Wed, 11 May 2011 12:33:23 +0100 4805021 http://www.medworm.com/index.php?rid=4805020&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21459470%26dopt%3DAbstract Authors: Yli-Mattila T, Ward TJ, O'Donnell K, Proctor RH, Burkin AA, Kononenko GP, Gavrilova OP, Aoki T, McCormick SP, Gagkaeva TY Production of type A trichothecenes has been reported in the closely related species Fusarium langsethiae and F. sporotrichioides. Here, we characterized a collection of Fusarium isolates from Siberia and the Russian Far East (hereafter Asian isolates) that produce high levels of the type A trichothecene T-2 toxin and are similar in morphology to the type A trichothecene-producing F. langsethiae, and to F. poae which often produces the type B trichothecene nivalenol. The Asian isolates possess unique macroscopic and microscopic characters and have a unique TG repeat in the nuclear ribosomal intergenic spacer (IGS rDNA) region. In Asian isolates, the TRI1-TR... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805020 Wed, 11 May 2011 12:33:18 +0100 4805020 http://www.medworm.com/index.php?rid=4805019&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21463910%26dopt%3DAbstract Authors: Steyn CE, Cameron M, Witthuhn RC Concentrated fruit products have a significant place in modern consumption markets and are valuable semi-prepared food components to the bakery, dairy, confectionary, canning, baby food, frozen food, distilling and beverage industries. There is continuous pressure on the beverage industry to improve the quality of concentrated fruit products in order for reconstituted fruit beverages to compete with beverages that are made from fresh fruits. In recent years, Alicyclobacillus spp. have become a major concern to the beverage industry worldwide as many high-acid, concentrated fruit products have been found to be contaminated with these spoilage microbes. The thermo-acidophilic nature of alicyclobacilli and highly resistant endospores allows for th... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805019 Wed, 11 May 2011 12:33:14 +0100 4805019 http://www.medworm.com/index.php?rid=4805018&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21463911%26dopt%3DAbstract In conclusion, we demonstrate that UV-C light is a useful extra bacteriocidal step and that it, as all disinfecting procedures, is hampered by the presence of organic material. PMID: 21463911 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805018 Wed, 11 May 2011 12:33:10 +0100 4805018 http://www.medworm.com/index.php?rid=4805017&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470706%26dopt%3DAbstract Authors: Leclercq A, Chenal-Francisque V, Dieye H, Cantinelli T, Drali R, Brisse S, Lecuit M The World Health Organization Collaborating Centre for Listeria (WHOCCL) has developed in 2004 a multiplex PCR assay that separates the 4 major Listeria monocytogenes serovars (1/2a, 1/2b, 1/2c, and 4b) into distinct PCR serogroups. A new PCR profile has been recently identified, constituted of amplified DNA fragments of prs, ORF2819, ORF2110 and lmo0737. Here we characterize 22 L. monocytogenes isolates of the WHOCCL collection with this PCR IVb variant 1 (IVb-v1) profile. The 22 isolates belong to the clinically predominant serovar 4b, exhibit 6 distinct pulsed-field gel electrophoresis ApaI/AscI combined profiles, and belong to 2 unrelated multilocus sequence types, indicating that the nove... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805017 Wed, 11 May 2011 12:33:05 +0100 4805017 http://www.medworm.com/index.php?rid=4805016&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474195%26dopt%3DAbstract Authors: Gamero A, Hernández-Orte P, Querol A, Ferreira V This work explores the ability of different yeast strains from different species of the genus Saccharomyces (S. cerevisiae, S. uvarum and S. kudriavzevii) and hybrids between these species to release or form varietal aroma compounds from fractions of grape odourless precursors. The de novo synthesis by the yeasts of some of the varietal aroma compounds was also evaluated. The study has shown that de novo synthesis affects some lipid derivatives, shikimic derivatives and terpenes in all species and hybrids, with some remarkable differences amongst them. The release or formation of aroma compounds from precursors was found to be strongly linked to the yeast or hybrid used, and the triple hybrid S. cerevisiae × S. bayanus × S. k... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805016 Wed, 11 May 2011 12:33:00 +0100 4805016 http://www.medworm.com/index.php?rid=4805015&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21477877%26dopt%3DAbstract Authors: Ross T, Olley J, McMeekin TA, Ratkowsky DA PMID: 21477877 [PubMed - in process] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805015 Wed, 11 May 2011 12:32:54 +0100 4805015 http://www.medworm.com/index.php?rid=4804997&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21550679%26dopt%3DAbstract Authors: Chen S, Li J, Saleh-Lakha S, Allen V, Odumeru J Listeria monocytogenes is the etiologic agent of listeriosis responsible for severe and fatal infections in humans. Listeria contamination occurs quite often in a wide range of foods due to its ubiquitous nature. Isolates need to be characterized to a strain level for accurate diagnosis of Listeria infection, epidemiological studies, investigation of outbreaks and effective prevention and control of food-borne listeriosis. The purpose of this research was to evaluate the multiple-locus variable number of tandem repeat analysis (MLVA) for sub-typing L. monocytogenes isolates in pure cultures and in food matrices. Two multiplex PCR assays were formulated to amplify six specific loci using fluorescently-labeled primers; and the ampl... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4804997 Thu, 21 Apr 2011 23:00:00 +0100 4804997 http://www.medworm.com/index.php?rid=4804996&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21550680%26dopt%3DAbstract Authors: Pablos M, Huys G, Cnockaert M, Rodríguez-Calleja JM, Otero A, Santos JA, García-López ML A collection of Aeromonas isolates obtained over a three-year period in the same geographic area (León, NW of Spain) was characterized by (GTG)(5)-PCR fingerprinting, amplified fragment length polymorphism (AFLP) analysis and gyrB gene sequence analysis. The isolates originated from human diarrheal stools (29 isolates), potable water (13 isolates), rabbit meat (13 isolates) and marine fish (5 isolates). The distribution of Aeromonas species varied with the strain source. Aeromonas caviae HG4 and Aeromonas media HG5 were predominant in clinical and water isolates, respectively, whereas motile Aeromonas salmonicida HG3 strains were most frequently found in fish and meat. Molecular typing... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4804996 Wed, 20 Apr 2011 23:00:00 +0100 4804996 http://www.medworm.com/index.php?rid=4804995&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21550681%26dopt%3DAbstract The objectives of this study were to evaluate the effect of the E. coli O157:H7 O-antigen on the cell's overall hydrophobicity, charge and ability to attach to cut edge and whole leaf iceberg lettuce surfaces. Three strains of E. coli O157:H7 (86-24 wild type; F-12, mutant lacking the O-antigen and pRFBE, plasmid for O157 gene reintroduced) were examined for their hydrophobicity, overall charge and ability to attach to lettuce. Overall, E. coli O157:H7 attached at higher levels to cut surfaces over whole leaf surfaces (P=0.008) for all strains and treatments. Additionally, the strain lacking the O-antigen (F12) - attached significantly less to lettuce (P=0.015) than the strains expressing the antigen (WT and pRFBE). Cells lacking the O antigen (strain F-12) were also significantly more hyd... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4804995 Wed, 20 Apr 2011 23:00:00 +0100 4804995 http://www.medworm.com/index.php?rid=4804998&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21546111%26dopt%3DAbstract Authors: Tian P, Yang D, Mandrell R Human norovirus (NoV) outbreaks are major food safety concerns. The virus has to be concentrated from food samples in order to be detected. PEG precipitation is the most common method to recover the virus. Recently, histo-blood group antigens (HBGA) have been recognized as receptors for human NoV, and have been utilized as an alternative method to concentrate human NoV for samples up to 40mL in volume. However, to wash off the virus from contaminated fresh food samples, at least 250mL of wash volume is required. Recirculating affinity magnetic separation system (RCAMS) has been tried by others to concentrate human NoV from large-volume samples and failed to yield consistent results with the standard procedure of 30min of recirculation at the default ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4804998 Thu, 14 Apr 2011 23:00:00 +0100 4804998 http://www.medworm.com/index.php?rid=4805006&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21529976%26dopt%3DAbstract Authors: Keskinen LA, Annous BA Numerous Escherichia coli O157:H7 outbreaks have been linked to consumption of fresh lettuce. The development of effective and easily implemented wash treatment could reduce such incidents. The purpose of this study was to evaluate the addition of food-grade detergents to sanitizer solutions for inactivation of E. coli O157:H7 on Romaine lettuce. Freshly-cut leaves of Romaine lettuce were dip-inoculated to achieve a final cell concentration of 7.8±0.2 log CFU/g, air-dried for 2h, and stored overnight at 4°C. Leaves were then washed for 2min in an experimental short chain fatty acid formulation (SCFA) or in one of the following solutions with or without 0.2% dodecylbenzenesulfonic acid or 0.2% sodium 2-ethyl hexyl sulfate: 1) deionized water; 2) 100ppm ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805006 Tue, 12 Apr 2011 23:00:00 +0100 4805006 http://www.medworm.com/index.php?rid=4805005&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21529977%26dopt%3DAbstract This study is the first report about strains of A. pullulans producing pectinases which are able to show good activity at low temperature. These pectinolytic strains could be of interest in wine production. PMID: 21529977 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805005 Tue, 12 Apr 2011 23:00:00 +0100 4805005 http://www.medworm.com/index.php?rid=4805004&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21531032%26dopt%3DAbstract Authors: Coffey B, Rivas L, Duffy G, Coffey A, Ross RP, McAuliffe O The efficacy of bacteriophages e11/2 and e4/1c as potential biocontrol agents for Escherichia coli O157:H7 in food applications was assessed under conditions relevant to the food chain environment. The stability of each phage was determined following exposure to varying environmental conditions (pH, temperature, water activity, and sodium chloride) and the ability of each phage to infect and reduce E. coli O157:H7 numbers under selected conditions was also examined. Both e11/2 and e4/1c significantly (p<0.05) reduced numbers of E. coli O157:H7 when exposed to pH values ranging from pH>4 to pH 9, temperatures from 4°C to 37°C, water activity values of 0.87 or 0.91 to 1.00 and NaCl concentrations of 1% to 2.5%. S... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805004 Tue, 12 Apr 2011 23:00:00 +0100 4805004 http://www.medworm.com/index.php?rid=4805001&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21531471%26dopt%3DAbstract Authors: Jaffrès E, Lalanne V, Macé S, Cornet J, Cardinal M, Sérot T, Dousset X, Joffraud JJ The spoilage potential of six bacterial species isolated from cooked and peeled tropical shrimps (Brochothrix thermosphacta, Serratia liquefaciens-like, Carnobacterium maltaromaticum, Carnobacterium divergens, Carnobacterium alterfunditum-like and Vagococcus penaei sp. nov.) was evaluated. The bacteria were inoculated into shrimps, packaged in a modified atmosphere and stored for 27days at 8°C. Twice a week, microbial growth, as well as chemical and sensory changes, were monitored during the storage period. The bacteria mainly involved in shrimp spoilage were B. thermosphacta, S. liquefaciens-like and C. maltaromaticum whose main characteristic odours were cheese-sour, cabbage-amine and che... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805001 Tue, 12 Apr 2011 23:00:00 +0100 4805001 http://www.medworm.com/index.php?rid=4805000&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21543129%26dopt%3DAbstract Authors: González A, Mas A The 16S-23S gene internal transcribed spacer sequence of sixty-four strains belonging to different acetic acid bacteria genera were analyzed, and phylogenetic trees were generated for each genera. The topologies of the different trees were in accordance with the 16S rRNA gene trees, although the similarity percentages obtained between the species was shown to be much lower. These values suggest the usefulness of including the 16S-23S gene internal transcribed spacer region as a part of the polyphasic approach required for the further classification of acetic acid bacteria. Furthermore, the region could be a good target for primer and probe design. It has also been validated for use in the identification of unknown samples of this bacterial group from wine vi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805000 Tue, 12 Apr 2011 23:00:00 +0100 4805000 http://www.medworm.com/index.php?rid=4804999&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21546110%26dopt%3DAbstract In this study, 75 yeast cultures were isolated from healthy pears from two Patagonian cold-storage packinghouses. Aureobasidium pullulans, Cryptococcus albidus, Cryptococcus difluens, Pichia membranifaciens, Pichia philogaea, Rhodotorula mucilaginosa and Saccharomyces cerevisiae yeast species were identified. Additionally, 13 indigenous isolates of Penicillium expansum and 10 isolates of Botrytis cinerea were obtained from diseased pears, characterized by aggressiveness and tested for sensitivity to postharvest fungicides. The yeasts were pre-selected for their ability to grow at low temperature. In a first biocontrol assay using the most aggressive and the most sensitive isolate of each pathogen, two epiphytic isolates of A. pullulans and R. mucilaginosa were the most promising isolates t... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4804999 Tue, 12 Apr 2011 23:00:00 +0100 4804999 http://www.medworm.com/index.php?rid=4805014&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21482440%26dopt%3DAbstract Authors: Ayeni FA, Sánchez B, Adeniyi BA, de Los Reyes-Gavilán CG, Margolles A, Ruas-Madiedo P The characterisation of 24 lactic acid bacteria (LAB) isolates from Nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. LAB isolates were identified by partial sequencing the 16S rRNA gene as belonging to the species Lactobacillus paracasei, Lactobacillus brevis and mainly Weissella confusa. At the end of a characterisation process, 2 L. paracasei and 2 W. confusa isolates were selected, and their resistance to a simulated gastrointestinal digestion and their ability to adhere to eukaryotic cell lines were assessed. The survival to the simulated gastrointestinal passage was higher whe... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805014 Fri, 08 Apr 2011 23:00:00 +0100 4805014 http://www.medworm.com/index.php?rid=4805002&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21531034%26dopt%3DAbstract This study represents the first proteomic analysis for a comparison of two A. carbonarius strains with different OTA production and will contribute to a better understanding of the molecular events involved in OTA biosynthesis. PMID: 21531034 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805002 Thu, 07 Apr 2011 23:00:00 +0100 4805002 http://www.medworm.com/index.php?rid=4805003&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21531033%26dopt%3DAbstract Authors: Domizio P, Romani C, Lencioni L, Comitini F, Gobbi M, Mannazzu I, Ciani M The use of non-Saccharomyces yeasts that are generally considered as spoilage yeasts, in association with Saccharomyces cerevisiae for grape must fermentation was here evaluated. Analysis of the main oenological characteristics of pure cultures of 55 yeasts belonging to the genera Hanseniaspora, Pichia, Saccharomycodes and Zygosaccharomyces revealed wide biodiversity within each genus. Moreover, many of these non-Saccharomyces strains had interesting oenological properties in terms of fermentation purity, and ethanol and secondary metabolite production. The use of four non-Saccharomyces yeasts (one per genus) in mixed cultures with a commercial S. cerevisiae strain at different S. cerevisiae/non-Saccharo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805003 Wed, 06 Apr 2011 23:00:00 +0100 4805003 http://www.medworm.com/index.php?rid=4805013&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21496933%26dopt%3DAbstract Authors: Phungamngoen C, Chiewchan N, Devahastin S The effects of selected drying methods, i.e., hot air drying, vacuum drying and low-pressure superheated steam drying (LPSSD), on the heat resistance of Salmonella attached on vegetable surface, which are data that have never been reported elsewhere, were investigated at drying temperatures of 50-70°C; vacuum drying and LPSSD were carried out at an absolute pressure of 10kPa. The selected Salmonella serovar, i.e., S. Anatum, was used as a test organism. Cabbage was used as a vegetable model to represent uneven natural surface. The results showed that drying methods had a significant effect on the drying kinetics as well as the destruction rate of Salmonella. Higher drying temperatures resulted in higher destruction rates of S. Anatum.... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805013 Thu, 31 Mar 2011 23:00:00 +0100 4805013 http://www.medworm.com/index.php?rid=4805009&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21497932%26dopt%3DAbstract Authors: Arroyo C, Cebrián G, Mackey BM, Condón S, Pagán R The effect of High Hydrostatic Pressure (HHP) on the survival of Cronobacter sakazakii was investigated. Deviations from linearity were found on the survival curves and the Mafart equation accurately described the kinetics of inactivation. Comparisons between strains and treatments were made based on the time needed for a 5-log(10) reduction in viable count. The ability of C. sakazakii to tolerate high pressure was strain-dependent with a 26-fold difference in resistance among four strains tested. Pressure resistance was greatest in the stationary growth phase and at the highest growth temperatures tested (30 and 37°C). Cells treated in neutral pH buffer were 5-fold more resistant than those treated at pH 4.0, and 8-fold mo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805009 Thu, 31 Mar 2011 23:00:00 +0100 4805009 http://www.medworm.com/index.php?rid=4805012&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21496934%26dopt%3DAbstract Authors: Buňková L, Buňka F, Pollaková E, Podešvová T, Dráb V The aim of this work was to study, under model conditions, combined effects of the concentration of lactose (0-1% w/v), NaCl (0-2% w/v) and aero/anaerobiosis on the growth and tyramine production in 3 strains of Lactococcus lactis subsp. lactis and 2 strains of L. lactis subsp. cremoris. The levels of the factors tested were chosen with respect to the conditions which can occur during the real process of natural cheese production, including the culture temperature (10±1°C). In all strains tested, tyrosine decarboxylation was most influenced by NaCl concentration; the highest production of tyramine was obtained within the culture with the highest (2% w/v) salt concentration applied. Two of the strains L. lactis subsp... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805012 Wed, 30 Mar 2011 23:00:00 +0100 4805012 http://www.medworm.com/index.php?rid=4805011&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21496935%26dopt%3DAbstract Authors: Brzonkalik K, Herrling T, Syldatk C, Neumann A The aim of this study was to determine the influence of different carbon and nitrogen sources on the production of the mycotoxins alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) by Alternaria alternata at 28°C using a semi-synthetic medium (modified Czapek-Dox broth) supplemented with nitrogen and carbon sources. Additionally the effect of shaken and static cultivation on mycotoxin production was tested. Initial experiments showed a clear dependency between nitrogen depletion and mycotoxin production. To assess whether nitrogen limitation in general or the type of nitrogen source triggers the production, various nitrogen sources including several ammonium/nitrate salts and amino acids were tested. I... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805011 Wed, 30 Mar 2011 23:00:00 +0100 4805011 http://www.medworm.com/index.php?rid=4805007&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21513995%26dopt%3DAbstract Authors: Vaerewijck MJ, Sabbe K, Baré J, Houf K The occurrence and diversity of free-living protozoa (FLP) on butterhead lettuce (Lactuca sativa L.) was investigated using four different sampling techniques (washing, swabbing, homogenization, and excising). FLP were recovered from all leaf samples (n=64), and cultures were FLP-positive after 1week. Identification of FLP was performed by light microscopy and sequencing of denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments. Bodo saltans, Spumella (-like) spp. and Cercozoa were the most common heterotrophic nanoflagellates. Amoebae belonged mainly to the Vannellida and Tubulinida. Colpoda steinii and Cyclidium glaucoma were the most common ciliates. The total number of FLP on middle leaves estimated by the M... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805007 Mon, 28 Mar 2011 23:00:00 +0100 4805007 http://www.medworm.com/index.php?rid=4805022&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450362%26dopt%3DAbstract Authors: De Vuyst L, Leroy F Inulin-type fructans are not digested and reach the human colon intact, where they are selectively fermented by the colon microbiota, in particular bifidobacteria. As a result, they are converted, directly or indirectly, to short-chain fatty acids and other organic acids, as well as gases, and lead to both bifidogenic and butyrogenic health-promoting effects. Bifidobacteria display phenotypic variation on strain level as to their capacity to degrade inulin-type fructans. Also, different chain lengths of inulin-type fructans may stimulate different subgroups within the bifidobacterial population. The end-metabolites of inulin-type fructan degradation by bifidobacteria reflect their growth rates on these polymers. Other colon bacteria are also able to degrade... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805022 Sun, 27 Mar 2011 23:00:00 +0100 4805022 http://www.medworm.com/index.php?rid=4805010&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21497408%26dopt%3DAbstract Authors: Bautista-Gallego J, Rodríguez-Gómez F, Barrio E, Querol A, Garrido-Fernández A, Arroyo-López FN In recent years, there has been an increasing interest in identifying and characterizing the yeast populations associated with diverse types of table olive elaborations because of the many desirable technological properties of these microorganisms. In this work, a total of 199 yeast isolates were directly obtained from industrial green table olive fermentations and genetically identified by means of a RFLP analysis of the 5.8S-ITS region and sequencing of the D1/D2 domains of the 26S rDNA gene. Candida diddensiae, Saccharomyces cerevisiae and Pichia membranifaciens were the most abundant yeast species isolated from directly brined Aloreña olives, while for Gordal and Manzanilla... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805010 Fri, 25 Mar 2011 00:00:00 +0100 4805010 http://www.medworm.com/index.php?rid=4805008&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21501884%26dopt%3DAbstract Authors: Kirs M, Depaola A, Fyfe R, Jones JL, Krantz J, Van Laanen A, Cotton D, Castle M A microbiological survey was conducted to determine the levels of total and pathogenic Vibrio parahaemolyticus (Vp) and Vibrio vulnificus (Vv) in Pacific oysters (Crassostrea gigas) collected from commercial growing areas in the North Island, New Zealand. The survey was intended to be geographically representative of commercial growing areas of Pacific oysters in New Zealand, while selecting the time frame most likely to coincide with the increased abundance of pathogenic vibrio species. Vp was detected in 94.8% of oyster samples examined (n=58) with a geometric mean concentration of 99.3MPN/g, while Vv was detected in 17.2% of oyster samples examined with a geometric mean concentration of 7.4MPN/g... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805008 Fri, 25 Mar 2011 00:00:00 +0100 4805008 http://www.medworm.com/index.php?rid=4805028&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439667%26dopt%3DAbstract In conclusion, this work clearly shows a link between bacterial cell density and the efficacy of CAP inactivation, making an important contribution to the understanding of this alternative food processing technology, which should be taken into account in both further studies and in the practical application of this technique to the food industry. PMID: 21439667 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4805028 Wed, 23 Mar 2011 00:00:00 +0100 4805028 http://www.medworm.com/index.php?rid=4643134&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21429610%26dopt%3DAbstract Authors: Fearnley E, Raupach J, Lagala F, Cameron S Varieties of Salmonella enterica are the second most commonly notified causes of gastroenteritis in Australia. Outbreaks of Salmonella infection are commonly linked to food, particularly foods containing chicken meat and eggs. A number of European countries have introduced interventions based on Salmonella surveillance systems in the food industry and these have led to subsequent decreases in notification rates in humans. A descriptive case-series of human Salmonella infections notified in metropolitan Adelaide, South Australia, was conducted in 2008. Human Salmonella serotypes identified were then compared to serotypes identified from a retail chicken and egg survey conducted over the same time period in Adelaide. Ninety-four human c... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4643134 Mon, 21 Mar 2011 00:00:00 +0100 4643134 http://www.medworm.com/index.php?rid=4643135&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21421274%26dopt%3DAbstract The objective of this work was to study the germination and inactivation of A. acidoterrestris and B. coagulans spores by high hydrostatic pressure (HP) treatment at temperatures up to 60°C and both at low and neutral pH. In a first experiment, spores suspended in buffers at pH 4.0, 5.0 and 7.0 were processed for 10min at different pressures (100-800MPa) at 40°C. None of these treatments caused any significant inactivation, except perhaps at 800MPa in pH 4.0 buffer where close to 1 log inactivation of B. coagulans was observed. Spore germination up to about 2 log was observed for both bacteria but occurred mainly in a low pressure window (100-300MPa) for A. acidoterrestris and only in a high pressure window (600-800MPa) for B. coagulans. In addition, low pH suppressed germination in A. a... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4643135 Sat, 19 Mar 2011 00:00:00 +0100 4643135 http://www.medworm.com/index.php?rid=4643136&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21411170%26dopt%3DAbstract Authors: Nualkaekul S, Charalampopoulos D The aim of the work was to study the survival of Lactobacillus plantarum NCIMB 8826 in model solutions and develop a mathematical model describing its dependence on pH, citric acid and ascorbic acid. A Central Composite Design (CCD) was developed studying each of the three factors at five levels within the following ranges, i.e., pH (3.0-4.2), citric acid (6-40g/L), and ascorbic acid (100-1000mg/L). In total, 17 experimental runs were carried out. The initial cell concentration in the model solutions was approximately 1×10(8)CFU/mL; the solutions were stored at 4°C for 6weeks. Analysis of variance (ANOVA) of the stepwise regression demonstrated that a second order polynomial model fits well the data. The results demonstrated that high pH and ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4643136 Mon, 14 Mar 2011 00:00:00 +0100 4643136 http://www.medworm.com/index.php?rid=4581109&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21382642%26dopt%3DAbstract Authors: Fricker-Feer C, Cernela N, Bolzan S, Lehner A, Stephan R In the last few years, various PCR based methods have been developed that enable detection of Cronobacter spp. to the genus and species level. Moreover, several real-time PCR based systems for detection of Cronobacter spp. are available, however, comparative evaluation studies are not available. The current study represents a comparative evaluation of three commercial diagnostic systems, namely the BAX® System PCR Assay Enterobacter sakazakii (DuPont, Qualicon, Wilmington, USA), the Assurance GDS™ Enterobacter sakazakii (BioControl, Bellvue, USA) and the foodproof® Enterobacter sakazakii Detection Kit (Biotecon Diagnostics, Potsdam, Germany) for the rapid identification of Cronobacter spp. Twenty-one target and non-t... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581109 Sat, 05 Mar 2011 00:00:00 +0100 4581109 http://www.medworm.com/index.php?rid=4581114&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21371767%26dopt%3DAbstract Authors: Witthuhn RC, Smit Y, Cameron M, Venter P Alicyclobacillus species are thermo-acidophilic, endospore-forming bacteria that are able to survive pasteurisation and have been implicated in a number of spoilage incidents involving acidic foods and beverages. The aim of this study was to compare three isolation methods used for the detection of Alicyclobacillus acidoterrestris and to investigate the influence of incubation temperature on the growth of A. acidoterrestris and A. acidocaldarius. Peach juice samples inoculated with A. acidoterrestris K47 were analysed using either the International Federation of Fruit Juice Producers (IFU) Method No. 12 (Method A), which involved spread plating onto Bacillus acidoterrestris (BAT) agar at pH 4.0; Method B, which involved pour plating usi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581114 Wed, 02 Mar 2011 00:00:00 +0100 4581114 http://www.medworm.com/index.php?rid=4581113&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21371768%26dopt%3DAbstract Authors: Hedderich R, Müller R, Greulich Y, Bannert N, Holland G, Kaiser P, Reissbrodt R Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10s, compared with short time hand plating (5s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to cont... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581113 Wed, 02 Mar 2011 00:00:00 +0100 4581113 http://www.medworm.com/index.php?rid=4581112&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21371769%26dopt%3DAbstract In this study, we have analyzed 593 foodborne STEC strains for their serotypes and for nine virulence genes (stx1, stx1c, stx1d, stx2, stx2b, stx2e, stx2g, E-hly and eae). The 593 STEC strains grouped into 215 serotypes, and 123 serotypes (57.2%) were represented each by only one STEC isolate. Fifteen serotypes (7.0%) were attributed to 198 (33.3%) of the 593 STEC strains. The foodborne STEC were grouped into different categories in relation to the species of the food producing animal (cattle, pigs, sheep, goats, red deer, wild-boar and hare). Univariate and multivariate statistical analyses revealed significant similarities between the animal origin of the food and the virulence markers of foodborne STEC. Significant associations (p<0.001) were found for stx1 and for stx2 with bovine m... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581112 Wed, 02 Mar 2011 00:00:00 +0100 4581112 http://www.medworm.com/index.php?rid=4581115&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21367479%26dopt%3DAbstract Authors: Söderholm H, Lindström M, Somervuo P, Heap J, Minton N, Lindén J, Korkeala H The relative expression of three cold shock protein coding genes (cspA, cspB and cspC) of Clostridium botulinum ATCC 3502 was studied with quantitative RT-PCR analysis following a cold shock shift from 37°C to 15°C. A significant increase in the relative expression of all three genes was observed upon the temperature downshift. To validate these findings, single-gene insertional inactivation of cspA, cspB and cspC was undertaken with the ClosTron gene knock-out system. In growth experiments, mutations in cspB or cspC, but not cspA, resulted in a cold-sensitive phenotype. No growth of the cspB mutant was observed at 15°C over a ten day period, whereas at 20°C the growth rate was 70% lower than t... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581115 Tue, 01 Mar 2011 00:00:00 +0100 4581115 http://www.medworm.com/index.php?rid=4581116&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21356564%26dopt%3DAbstract Authors: Hauge SJ, Wahlgren M, Røtterud OJ, Nesbakken T Although hot water pasteurisation of carcasses is accepted as a general intervention in USA, this is not the case in Europe. The aims of this study were (i) to evaluate the microbiological effects of hot water pasteurisation of lamb carcasses, both after slaughtering and dressing and following subsequent chilling and storage; (ii) to discuss hot water pasteurisation from a public health and cost-benefit perspective; (iii) to discuss the benefits of hot water pasteurisation compared with use of separate meat processing streams for high-risk carcasses; (iv) to evaluate the use of recycled hot water in a hygienic context and in relation to EU regulations; and (v) to consider the technological and sensory aspects of hot water pasteur... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581116 Sat, 26 Feb 2011 00:00:00 +0100 4581116 http://www.medworm.com/index.php?rid=4581117&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21354646%26dopt%3DAbstract This study aims at investigating whether the conditions of growth of the vegetative cells, prior to sporulation, could affect spore heat resistance. For that purpose, wet-heat resistance of Bacillus weihenstephanensis KBAB4 spores, produced via a two-step sporulation process, was determined from vegetative cells harvested at four different stages of the growth kinetics, i.e. early exponential phase, late exponential phase, transition phase or early stationary phase. To assess the impact of the temperature on spore heat resistance, sporulation was performed at 10°C, 20°C and 30°C from cells grown during a continuous or a discontinuous temperature process, differentiating or not the growth and sporulation temperatures. Induction of sporulation seems possible for a large range of growth st... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581117 Fri, 25 Feb 2011 00:00:00 +0100 4581117 http://www.medworm.com/index.php?rid=4581120&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21353718%26dopt%3DAbstract Authors: Fenicia L, Fach P, van Rotterdam BJ, Anniballi F, Segerman B, Auricchio B, Delibato E, Hamidjaja RA, Wielinga PR, Woudstra C, Agren J, De Medici D, Knutsson R A real-time PCR method for detection and typing of BoNT-producing Clostridia types A, B, E, and F was developed on the framework of the European Research Project "Biotracer". A primary evaluation was carried out using 104 strains and 17 clinical and food samples linked to botulism cases. Results showed 100% relative accuracy, 100% relative sensitivity, 100% relative specificity, and 100% selectivity (inclusivity on 73 strains and exclusivity on 31 strains) of the real-time PCR against the reference cultural method combined with the standard mouse bioassay. Furthermore, a ring trial study performed at four different Europ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581120 Thu, 24 Feb 2011 00:00:00 +0100 4581120 http://www.medworm.com/index.php?rid=4581121&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21353322%26dopt%3DAbstract Authors: Liu J, Wisniewski M, Droby S, Vero S, Tian S, Hershkovitz V The effect of H(2)O(2)-induced oxidative stress on the viability of the yeast antagonist, Cystofilobasidium infirmominiatum, as well as the effect of exogenous glycine betaine (GB) on yeast viability under oxidative stress, was determined. GB treatment improved the tolerance of C. infirmominiatum to oxidative stress. Compared to untreated control yeast cells, GB-treated cells showed less accumulation of reactive oxygen species (ROS) and a lower level of protein oxidation in response to oxidative stress. Additionally, GB-treated yeast exhibited greater biocontrol activity against Penicillium expansum and a faster growth in wounds of apple fruits stored at 25°C compared to the performance of untreated yeast. The activi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581121 Wed, 23 Feb 2011 00:00:00 +0100 4581121 http://www.medworm.com/index.php?rid=4581110&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21377750%26dopt%3DAbstract Authors: Cremonesi P, Vanoni L, Morandi S, Silvetti T, Castiglioni B, Brasca M A pentaplex PCR assay for the rapid, selective and simultaneous detection of Lactobacillus helveticus, L. delbrueckii subsp. lactis, L. delbrueckii subsp. bulgaricus, Streptococcus thermophilus, and L. fermentum, was developed. The target sequences were a group of genes coding for beta-galactosidase production (S. thermophilus and L. delbrueckii subsp. bulgaricus), for cell-enveloped associated proteinase synthesis (L. helveticus), for dipeptide transport system production (L. delbrueckii subsp. lactis) and for arginine-ornithine antiporter protein production (L. fermentum). The analytical specificity of the assay was evaluated with 5 reference strains and 140 lactic acid bacterial strains derived from raw m... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581110 Tue, 22 Feb 2011 00:00:00 +0100 4581110 http://www.medworm.com/index.php?rid=4581119&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21354644%26dopt%3DAbstract Authors: Sadat-Mekmene L, Genay M, Atlan D, Lortal S, Gagnaire V Lactobacillus helveticus is a lactic acid bacterium very used in fermented milks and cheese. The rapid growth of L. helveticus in milk is supported by an efficient cell envelope proteinase (CEP) activity, due to subtilisin-like serine proteases. These enzymes play also crucial roles in texture and flavor formation in dairy products as well as in generating in situ bioactive peptides. In L. helveticus, several genes encoding putative CEPs were detected and characterized by a large intraspecific diversity; little is known about regulation of expression of CEP-encoding genes. Anchored at the bacterial surface, CEPs are large-sized enzymes (>150kDa) hydrolyzing β- and α(s1)-casein as well. Substrate cleavages occur after... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581119 Wed, 02 Feb 2011 00:00:00 +0100 4581119 http://www.medworm.com/index.php?rid=4581118&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21354645%26dopt%3DAbstract Authors: Kim KY, Park JH, Kwak HS, Woo GJ Sixteen Salmonella strains resistant to nalidixic acid isolated from kimbab, the most popular ready-to-eat (RTE) food in Korea, and chicken meat were selected for this study. The resistant strains were shown to have high minimal inhibitory concentrations (MICs) against nalidixic acid (512~4096μg/mL). Among them, 4 Salmonella enterica serovar Haardt isolates showed multi-drug resistance (MDR) patterns with reduced susceptibility to fluoroquinolone (0.5μg/mL of ciprofloxacin MICs). The mechanisms of quinolone resistance in the nalidixic acid resistant strains were characterized by PCR and sequence analysis. The presence of plasmid-mediated quinolone resistance (PMQR) genes and amino acid changes in the quinolone resistance determining region (Q... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581118 Wed, 02 Feb 2011 00:00:00 +0100 4581118 http://www.medworm.com/index.php?rid=4581111&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21377228%26dopt%3DAbstract This study endeavored to investigate the variability of bacteria and fungi present during the fermentation process of the light-fragranced distilled liquor known as Fen liquor. To accomplish this, we used a combination of clone libraries of 16S rRNA genes, bar-coded pyrosequencing of the internal transcribed spacer region 1 (ITS1), and quantitative real-time PCR (qPCR). Fifteen families of bacteria and six families of fungi were detected. More than 91% of 16S rRNA gene sequences could be assigned to the family Lactobacillaceae, which were then classified to eight different operational taxonomic units (OTUs), based on a 3% cut-off. The most abundant OTU which contributed to 51% of the total 16S rRNA gene sequences was affiliated with Lactobacillus acetotolerans and had a significantly simil... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4581111 Wed, 02 Feb 2011 00:00:00 +0100 4581111 http://www.medworm.com/index.php?rid=4527318&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329996%26dopt%3DAbstract This study shows that the amount and the ratio 4VP/4EP produced by LAB are greatly affected by certain environmental and medium composition factors. The behaviour of the bacteria seems to be driven by the intracellular NAD(+)/NADH balance. PMID: 21329996 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527318 Wed, 02 Feb 2011 00:00:00 +0100 4527318 http://www.medworm.com/index.php?rid=4527314&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334756%26dopt%3DAbstract In conclusion, the current work shows the protective effect of biofilm formation, salt and osmoadaptation on the desiccation survival of L. monocytogenes, which in turn increases the potential for cross-contamination during food processing. PMID: 21334756 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527314 Wed, 02 Feb 2011 00:00:00 +0100 4527314 http://www.medworm.com/index.php?rid=4527313&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334757%26dopt%3DAbstract In this study fattening pigs were monitored on farms and in the abattoir for Salmonella prevalence. The samples with the highest prevalence at slaughter should be identified with special attention to the distribution of Salmonella serovars on farms in comparison to those in slaughtered pigs. Another aim was to monitor whether high serological antibody responses in pigs are in accordance with the specific Salmonella serovars in tissues. From 3418 farm faecal samples, 191 were Salmonella positive (5.58%), whereas from slaughtered pigs 330 out of 2494 analysed samples were Salmonella positive (13.2%) with the highest prevalence in the caecal content (124/499=24.9%). The chi-square test for homogeneity between the serovars found on farms and in the different types of samples at slaughter was i... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527313 Wed, 02 Feb 2011 00:00:00 +0100 4527313 http://www.medworm.com/index.php?rid=4527311&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21345511%26dopt%3DAbstract Authors: Xu A, Wang Y, Wen J, Liu P, Liu Z, Li Z Chinese Fuzhuan brick-tea is a unique microbial fermented tea characterized by a period of fungal growth during its manufacturing process. The aim of the present study was to characterize, both physicochemically and microbiologically, traditional industrial production processes of Fuzhuan brick-tea. Fermenting tea samples were collected from the largest manufacturer. Physicochemical analyses showed that the low water content in the tea substrates provided optimal growth conditions for xerophilic fungi. The fungal communities existing in tea materials, fermenting tea, and stored teas were monitored by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) targeting the D1 region of the 26S rRNA genes, followed by s... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527311 Wed, 02 Feb 2011 00:00:00 +0100 4527311 http://www.medworm.com/index.php?rid=4527310&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21349598%26dopt%3DAbstract Authors: Qin SS, Wu CM, Wang Y, Jeon B, Shen ZQ, Wang Y, Zhang Q, Shen JZ The aim of this study was to determine the prevalence, antimicrobial resistance and molecular epidemiology of Campylobacter coli isolated from swine in China. A total of 190 C. coli isolates obtained from two slaughter houses and ten conventional pig farms in Shandong (SD, n=95) and Ningxia (NX, n=95) provinces were tested for their susceptibility to 14 antimicrobials. A high prevalence (>95%) of ciprofloxacin and tetracycline-resistant strains was observed in both SD and NX. The erythromycin and clindamycin resistance rates of C. coli from NX (ERY: 54.7% CLI: 43.2%) were higher than those from SD (ERY: 37.9%, CLI: 35.8%). A significant difference (P<0.05) was observed in erythromycin resistance rate, but n... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527310 Wed, 02 Feb 2011 00:00:00 +0100 4527310 http://www.medworm.com/index.php?rid=4527320&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320732%26dopt%3DAbstract Authors: Jasson V, Baert L, Uyttendaele M The capacity to detect low levels of healthy and sub-lethally injured Salmonella enterica cells in chocolate by two alternative rapid detection methods iQ-Check(TM)Salmonella II real-time PCR (Bio-Rad) and VIDAS® Easy SLM (BioMérieux) was assessed and compared with ISO 6579:2005. Chocolate, a low moisture food known to support the survival of Salmonella, was challenged as food matrix. Buffered peptone water (BPW) did not support the recovery of low levels of sub-lethally injured S. enterica independent of the detection method, while BPW supplemented with milk powder enabled detection by the three examined methods. However, inhibition of real-time PCR was observed since for one out of three repetitions of chocolate inoculated with a low number... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527320 Mon, 31 Jan 2011 00:00:00 +0100 4527320 http://www.medworm.com/index.php?rid=4527319&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329995%26dopt%3DAbstract Authors: Delgado S, Suárez A, Mayo B Forty two Propionibacterium isolates were recovered from biopsy samples of the gastric mucosa of eight out of 12 healthy people. Of these, 41 were identified as belonging to Propionibacterium acnes; the remaining isolate was identified as belonging to Propionibacterium granulosum. Repetitive extragenic palindromic (REP)-PCR typing suggested that up to four strains might be present in the mucosa of the same individual. Sequence analysis of either recA, tly or camp5 genes of P. acnes isolates revealed two distinct phylogenetic lineages. As per the recA, most isolates belonged to type I, while the remainder of the isolates belonged to type II. Phenotypic analyses of representative isolates showed the different strains to have diverse biochemical prope... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527319 Mon, 31 Jan 2011 00:00:00 +0100 4527319 http://www.medworm.com/index.php?rid=4527317&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329997%26dopt%3DAbstract Authors: Monfort S, Gayán E, Condón S, Raso J, Alvarez I This paper is an evaluation of the lethal effectiveness of a successive application of pulsed electric fields (PEFs) and heat treatment in liquid whole egg (LWE) in the presence of different additives on the population of Salmonella serovar Enteritidis. Synergistic reductions of the Salmonella Enteritidis population were observed when LWE samples containing additives were treated with PEF (25kV/cm; 100 and 200kJ/kg), heat (55°C), or PEF followed by heat. The presence of additives, such as 10mM EDTA or 2% triethyl citrate, increased the PEF lethality 1 log(10) cycle and generated around 1.5log(10) cycles of cell damage, resulting in the reduction of undamaged cells of 4.4 and 3.1log(10) cycles, respectively. The application of ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527317 Mon, 31 Jan 2011 00:00:00 +0100 4527317 http://www.medworm.com/index.php?rid=4527315&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21333369%26dopt%3DAbstract This study confirmed the need of consideration of ECM binding and biogenic amine formation in the safety assessment of CNS used in the production of fermented foods. PMID: 21333369 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527315 Fri, 28 Jan 2011 00:00:00 +0100 4527315 http://www.medworm.com/index.php?rid=4527322&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320730%26dopt%3DAbstract Authors: Tian J, Ban X, Zeng H, He J, Huang B, Wang Y The essential oil extracted from the fruits of Cicuta virosa L. var. latisecta Celak was tested in vitro and in vivo against four foodborne fungi, Aspergillus flavus, Aspergillus oryzae, Aspergillus niger, and Alternaria alternata. Forty-five different components accounting for 98.4% of the total oil composition were identified by gas chromatography-mass spectrometry. The major components were γ-terpinene (40.92%), p-cymene (27.93%), and cumin aldehyde (21.20%). Antifungal activity was tested by the poisoned food technique against the four fungi. Minimum inhibitory concentration against the fungi was 5μL/mL and percentage inhibition of mycelial growth was determined at day 9. The essential oil had a strong inhibitory effect on spo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527322 Wed, 26 Jan 2011 00:00:00 +0100 4527322 http://www.medworm.com/index.php?rid=4527321&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320731%26dopt%3DAbstract Authors: Cronin M, Ventura M, Fitzgerald GF, van Sinderen D Members of the genus Bifidobacterium were first described over a century ago and were quickly associated with a healthy intestinal tract due to their numerical dominance in breast-fed babies as compared to bottle-fed infants. Health benefits elicited by bifidobacteria to its host, as supported by clinical trials, have led to their wide application as probiotic components of health-promoting foods, especially in fermented dairy products. However, the relative paucity of genetic tools available for bifidobacteria has impeded development of a comprehensive molecular understanding of this genus. In this review we present a summary of current knowledge on bifidobacterial metabolism, classification, physiology and genetics and outli... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527321 Wed, 26 Jan 2011 00:00:00 +0100 4527321 http://www.medworm.com/index.php?rid=4527312&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21342711%26dopt%3DAbstract Authors: Sela DA A promising strategy to improve health is the rational manipulation of one's beneficial microbiota via dietary interventions. This is observed in nature where specific bifidobacteria utilize human milk oligosaccharides (HMOs) that are encountered within the breast-fed infant colon. Bifidobacterium longum subsp. infantis is regarded as the archetypical HMO consumer associated with the developing neonate. This review summarizes the known molecular mechanisms underlying HMO utilization, as determined for bifidobacterial commensals. In addition, future directions of HMO research are discussed with an emphasis on physiological, ecological and clinical approaches to understand bifidobacterial utilization of this intriguing substrate. PMID: 21342711 [PubMed - as supplied ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527312 Wed, 26 Jan 2011 00:00:00 +0100 4527312 http://www.medworm.com/index.php?rid=4527324&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316116%26dopt%3DAbstract In this study, 57 pandemic strains, 27 pathogenic strains (tdh or trh positive) and 36 nonpathogenic strains isolated from China were analyzed with MLST. Forty-seven unique sequence types, one clonal complex (CC) and one doublet (D) were identified by eBURST and Mega4 analyses. CC corresponded to not only the known O3:K6 pandemic clone (including ST-3, ST-192, ST-227) but nonpathogenic clone (including ST-3, S-T2, ST-196, ST-220, ST-226). ST-3 was the founder of the complex. STs of the isolates were not inevitably associated with the presence or number of the accessory genes or the serotypes of the isolates. The ancestor strain of O3:K6 pandemic clone was originated from an environmental nonpathogenic O3:K6, ST-3 strain. The pandemic O3:K6 clone was developed from this strain in approximat... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527324 Sat, 22 Jan 2011 00:00:00 +0100 4527324 http://www.medworm.com/index.php?rid=4527325&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315469%26dopt%3DAbstract Authors: Nisiotou AA, Rantsiou K, Iliopoulos V, Cocolin L, Nychas GJ Grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. In the present study, bacterial species associated with sound and Botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. Isolates were identified by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequence analysis of partial 16S rRNA gene. Comparable counts were recorded between Botrytis-infected and sound grape samples. In all cases, the majority of isolates belonged to different species of Enterobacteriaceae. The dominant species in the vineyard was Klebsiella oxytoca that was found in different combinations with Citrobacter freundii, Enterobac... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527325 Fri, 21 Jan 2011 00:00:00 +0100 4527325 http://www.medworm.com/index.php?rid=4527316&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329998%26dopt%3DAbstract Authors: Resch MS, Nerz C, Rosenstein R, Götz F, Hertel C Aim of the work was to design a polynucleotide based DNA microarray as screening tool to detect genes in food associated coagulase-negative staphylococci (CNS). A focus was laid on genes with potential health concern and technological relevance. The microarray contained 220 probes for genes encoding antibiotic resistances, hemolysins, toxins, amino acid decarboxylases (e.g. biogenic amine formation), binding proteins to extracellular matrix (ECM), lipases, proteases, stress response factors, or nitrate dissimilation. Hybridization of genomic DNA isolated from 32 phenotypically characterized CNS permitted to detect numerous genes, corresponding with the phenotype. However, numerous hybridization signals were obtained for genes w... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527316 Fri, 21 Jan 2011 00:00:00 +0100 4527316 http://www.medworm.com/index.php?rid=4527323&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320729%26dopt%3DAbstract Authors: Reynoso MM, Ramirez ML, Torres AM, Chulze SN Argentina is the fourth largest exporter of wheat in the world. The main pathogen associated with Fusarium Head Blight (FHB) of wheat in Argentina is Fusarium graminearum lineage 7 also termed F. graminearum sensu stricto in the F. graminearum species complex, which can produce the Type B trichothecenes, usually deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). We used a multiplex PCR assay of Tri3, Tri7, and Tri13 to determine the trichothecene genotype of 116 strains F. graminearum collected from three locations in Argentina and then verified the chemotype by chemical analysis. PCR assays and chemical analyses gave the same results for all strains that produced trichothecenes. Most strains (&gt... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4527323 Thu, 20 Jan 2011 00:00:00 +0100 4527323 http://www.medworm.com/index.php?rid=4472614&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21296447%26dopt%3DAbstract Authors: Fusco V, Quero GM, Stea G, Morea M, Visconti A An extensive use of Weissella (W.) confusa is currently being made for the production of a variety of fermented foods and beverages although some strains of this species have emerged as opportunistic pathogens for humans and animals. Nevertheless, no rapid methods are available for the reliable identification of W. confusa. We developed a novel PCR using AFLP (Amplified Fragment Length Polymorphism)-derived primers for the rapid and unequivocal identification of W. confusa. Fluorescent AFLP of 30 strains of W. confusa, Leuconostoc citreum, Lactobacillus (Lb.) brevis, Lb. rossiae, Lb. plantarum and Lb. buchneri allowed us to detect, purify and sequence several W. confusa specific AFLP fragments. The homology search in BLAST of a 30... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472614 Tue, 18 Jan 2011 00:00:00 +0100 4472614 http://www.medworm.com/index.php?rid=4472615&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21296446%26dopt%3DAbstract Authors: Savard P, Lamarche B, Paradis ME, Thiboutot H, Laurin E, Roy D This randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) on fecal bacterial counts of healthy adults. Fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. Yoptimal (10(9)cfu/100g of BB-12 and LA-5 and 40mg of green tea extract) and 3. Yoptimal-10 (10(10)cfu/100g of BB-12, 10(9)cfu/100g of LA-5 and 40mg of green tea extract). These yoghurt products also contained Lactobacillus delbrueckii subsp. bulgaricus (10(7)cfu/100g) and Streptococcus ther... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472615 Sat, 15 Jan 2011 00:00:00 +0100 4472615 http://www.medworm.com/index.php?rid=4472613&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21303723%26dopt%3DAbstract Authors: Somma S, Pose G, Pardo A, Mulè G, Pinto VF, Moretti A, Logrieco AF Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to eac... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472613 Sat, 15 Jan 2011 00:00:00 +0100 4472613 http://www.medworm.com/index.php?rid=4472627&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21269720%26dopt%3DAbstract Authors: Ongeng D, Vasquez GA, Muyanja C, Ryckeboer J, Geeraerd AH, Springael D Surface contamination and internalisation of Escherichiacoli O157:H7 and Salmonella Typhimurium in cabbage leaf tissues at harvest (120days post-transplantation) following amendment of contaminated bovine manure to soil at different times during crop cultivation were investigated under tropical field conditions in the Central Agro-Ecological Zone of Uganda. Fresh bovine manure inoculated with rifampicin-resistant derivatives of non-virulent strains of E. coli O157:H7 and S. Typhimurium was incorporated into the soil to achieve inoculum concentrations of 4 and 7 log CFU/g at the point of transplantation, 56 or 105days post-transplantation of cabbage seedlings. Frequent sampling of the soil enabled the accura... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472627 Fri, 14 Jan 2011 00:00:00 +0100 4472627 http://www.medworm.com/index.php?rid=4472616&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21295882%26dopt%3DAbstract Authors: García-Ruiz A, Moreno-Arribas MV, Martín-Álvarez PJ, Bartolomé B This paper reports a comparative study of the inhibitory potential of 18 phenolic compounds, including hydroxybenzoic acids and their derivatives, hydroxycinnamic acids, phenolic alcohols and other related compounds, stilbenes, flavan-3-ols and flavonols, on different lactic acid bacteria (LAB) strains of the species Oenococcus oeni, Lactobacillus hilgardii and Pediococcus pentosaceus isolated from wine. In general, flavonols and stilbenes showed the greatest inhibitory effects (lowest IC(50) values) on the growth of the strains tested (0.160-0.854 for flavonols and 0.307-0.855g/L for stilbenes). Hydroxycinnamic acids (IC(50)>0.470g/L) and hydroxybenzoic acids and esters (IC(50)>1g/L) exhibited medium i... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472616 Fri, 14 Jan 2011 00:00:00 +0100 4472616 http://www.medworm.com/index.php?rid=4472625&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276628%26dopt%3DAbstract In this study, we generated monoclonal antibodies (mAbs) against purified TRH recombinant protein of pathogenic V. parahaemolyticus. Sandwich enzyme-linked immunosorbent assays (ELISA) using the hybridoma clone 4B10 showed higher sensitivity of detection compared to other clones. Using mAb 4B10 based sandwich ELISA, we could detect pathogenic V. parahaemolyticus in 41.18% (14 out of 34) of the seafood samples analyzed. PCR targeting the toxR gene showed the presence of V. parahaemolyticus in 64.7% (22 out of 34) seafood samples. Further, PCR targeting the virulence genes showed that 6 seafood samples harboured the tdh gene while 9 harboured the trh gene indicating the presence of pathogenic V. parahaemolyticus. Our results show that mAb 4B10 sandwich ELISA developed in this study could be ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472625 Thu, 13 Jan 2011 00:00:00 +0100 4472625 http://www.medworm.com/index.php?rid=4472624&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276629%26dopt%3DAbstract Authors: Zhou G, Wen S, Liu Y, Li R, Zhong X, Feng L, Wang L, Cao B The safety and accessibility of drinking water are major concerns throughout the world. Consumption of water contaminated with infectious agents, toxic chemicals or radiological hazards represents a significant health risk and is strongly associated with mortality. Therefore, we have developed an oligonucleotide-based microarray using the sequences of 16S-23S rDNA internal transcribed spacer regions (ITS) and the gyrase subunit B gene (gyrB) found in the most prevalent and devastating waterborne pathogenic agents. This new diagnostic contains 26 specific probes and can simultaneously detect Aeromonas hydrophila, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Staph... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472624 Thu, 13 Jan 2011 00:00:00 +0100 4472624 http://www.medworm.com/index.php?rid=4472629&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21269718%26dopt%3DAbstract In conclusion, the oscillation method developed shows to be highly efficacious in reducing foodborne pathogens on lettuce and spinach leaves. PMID: 21269718 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472629 Wed, 12 Jan 2011 00:00:00 +0100 4472629 http://www.medworm.com/index.php?rid=4472628&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21269719%26dopt%3DAbstract Authors: Fernández-Ortuño D, Atkins SL, Fraaije BA Contamination of cereals with mycotoxins such as beauvericin (BEA), enniatins (Ens) and moniliformin (MON) is mainly caused by Fusarium avenaceum and F. tricinctum. This is a world-wide problem which requires rapid and sensitive detection methods. To allow for high throughput screening of large numbers of samples, a diagnostic PCR method was developed for the simultaneous detection of F. avenaceum and F. tricinctum. The interspecific divergence found in the Fusarium-specific CYP51C gene was used to design species-specific PCR primers. The specificity of the assay was demonstrated for DNA samples extracted from a wide range of Fusarium species belonging to the Fusarium head blight (FHB) complex, as well as for naturally-infected grain... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472628 Wed, 12 Jan 2011 00:00:00 +0100 4472628 http://www.medworm.com/index.php?rid=4472623&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276630%26dopt%3DAbstract Authors: Tanaka K, Yamaguchi N, Baba T, Amano N, Nasu M Aseptically prepared cold drinks based on tea have become popular worldwide. Contamination of these drinks with harmful microbes is a potential health problem because such drinks are kept free from preservatives to maximize aroma and flavour. Heat-tolerant conidia and ascospores of fungi can survive pasteurization, and need to be detected as quickly as possible. We were able to rapidly and accurately detect low numbers of conidia and ascospores in tea-based drinks using fluorescent staining followed by an automated counting system. Conidia or ascospores were inoculated into green tea and oolong tea, and samples were immediately filtered through nitrocellulose membranes (pore size: 0.8μm) to concentrate fungal propagules. These we... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472623 Wed, 12 Jan 2011 00:00:00 +0100 4472623 http://www.medworm.com/index.php?rid=4472621&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276632%26dopt%3DAbstract In this study, conducted at five slaughterhouses, individual pigs were sampled and followed up from stunning to cooling down of the carcasses. In this way, Salmonella prevalence and possible risk points were described. At the lairage area, pens were sampled using overshoes. At stunning and bleeding, pigs were individually identified and subsequently swabs were taken of the oral cavity and the carcass after polishing, splitting and forced chilling. Additionally, duodenum, ileum, rectum and mesenteric lymph nodes were extracted and samples were taken of the scalding water. All samples were submitted to Salmonella isolation and Salmonella isolates were serotyped and genotyped by pulsed-field gel electrophoresis (PFGE). Of all samples taken (n=1953), 14.1% were Salmonella positive. The prevale... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472621 Wed, 12 Jan 2011 00:00:00 +0100 4472621 http://www.medworm.com/index.php?rid=4472620&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276633%26dopt%3DAbstract This study was performed to compare the effectiveness of individual treatments (ultrasound and organic acids) and their combination on reducing foodborne pathogens on organic fresh lettuce. Lettuce leaves were inoculated with a cocktail of three strains each of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes and treated with ultrasound (40kHz) alone, organic acids (0.3, 0.5, 0.7, 1.0, and 2.0% - malic acid, lactic acid, and citric acid) alone and combined with ultrasound and organic acids for 5min. For all 3 pathogens, the combined treatment of ultrasound and organic acids resulted in additional 0.8 to 1.0 log reduction compared to individual treatments, without causing significant quality change (color and texture) on lettuce during 7day storage. The maximum r... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472620 Wed, 12 Jan 2011 00:00:00 +0100 4472620 http://www.medworm.com/index.php?rid=4472618&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21295367%26dopt%3DAbstract Authors: Chorianopoulos N, Giaouris E, Grigoraki I, Skandamis P, Nychas GJ The aim of this study was to investigate the potential effect of adaptive stationary phase acid tolerance response (ATR) of Listeria monocytogenes Scott A cells on their attachment to stainless steel (SS) under low pH or/and high salt conditions and on the subsequent resistance of sessile cells to strong acid challenge. Nonadapted or acid-adapted stationary-phase L. monocytogenes cells were used to inoculate (ca. 10(8) CFU/ml) Brain Heart (BH) broth (pH 7.4, 0.5% w/v NaCl) in test tubes containing vertically placed SS coupons (used as abiotic substrates for bacterial attachment). Incubation was carried out at 16°C for up to 15days, without any nutrient refreshment. L. monocytogenes cells, prepared as described ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472618 Tue, 11 Jan 2011 00:00:00 +0100 4472618 http://www.medworm.com/index.php?rid=4472626&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21272949%26dopt%3DAbstract Authors: Dang TD, Vermeulen A, Mertens L, Geeraerd AH, Van Impe JF, Devlieghere F In a previous study on Zygosaccharomyces bailii, three growth/no growth models have been developed, predicting growth probability of the yeast at different conditions typical for acidified foods (Dang, T.D.T., Mertens, L., Vermeulen, A., Geeraerd, A.H., Van Impe, J.F., Debevere, J., Devlieghere, F., 2010. Modeling the growth/no growth boundary of Z. bailii in acidic conditions: A contribution to the alternative method to preserve foods without using chemical preservatives. International Journal of Food Microbiology 137, 1-12). In these broth-based models, the variables were pH, water activity and acetic acid, with acetic acid concentration expressed in volume % on the total culture medium (i.e., broth). T... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472626 Sat, 08 Jan 2011 00:00:00 +0100 4472626 http://www.medworm.com/index.php?rid=4472622&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276631%26dopt%3DAbstract This study aimed to determine the impact of glycated pea proteins on the intestinal bacteria from a healthy human. The analyses were conducted with the use of experimental batch-type simulator models imitating human intestinal conditions. The glycated pea proteins affected the growth of gut commensal bacteria, particularly lactobacilli and bifidobacteria, whose levels increased significantly. There was a corresponding shift in the bacterial metabolites with increased levels of the short chain fatty acids (SCFAs); acetate, propionate lactate and butyrate. Intestinal bacteria were able to utilize these pea proteins thus indicating that the energy encrypted in glycated pea proteins, partially inaccessible for gastric enzymes, may be salvaged by gut microbiota. Such changes in microbial compos... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472622 Sat, 08 Jan 2011 00:00:00 +0100 4472622 http://www.medworm.com/index.php?rid=4472619&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21276634%26dopt%3DAbstract Authors: Carpentier B, Cerf O To understand why Listeria monocytogenes may persist in food industry equipment and premises, notably at low temperature, scientific studies have so far focused on adhesion potential, biofilm forming ability, resistance to desiccation, acid and heat, tolerance to increased sublethal concentration of disinfectants or resistance to lethal concentrations. Evidence from studies in processing plants shows that the factors associated with the presence of L. monocytogenes are those that favor growth. Interestingly, most conditions promoting bacterial growth were shown, in laboratory assays, to decrease adhesion of L. monocytogenes cells. Good growth conditions can be found in so-called harborage sites, i.e. shelters due to unhygienic design of equipment and premi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472619 Sat, 08 Jan 2011 00:00:00 +0100 4472619 http://www.medworm.com/index.php?rid=4472617&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21295368%26dopt%3DAbstract Authors: Hardy CG, Lackey LG, Cannon J, Price LB, Silbergeld EK Campylobacteriosis is the most common antecedent infection leading to the development of inflammatory neuropathies including Guillain Barré syndrome (GBS) and Miller Fisher syndrome (MFS), with alterations in surface proteins and genetic polymorphisms conferring increased risk. Poultry is the most common source of C. jejuni infection in industrialized countries, including the US. There are no data on the prevalence on consumer poultry products of various strains of C. jejuni, including those hypothesized to be associated with neuropathy. To study this, C. jejuni was isolated from fresh broiler chicken products purchased from grocery stores in the Baltimore area. LOS subtypes and specific genetic polymorphisms were determi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4472617 Sat, 08 Jan 2011 00:00:00 +0100 4472617 http://www.medworm.com/index.php?rid=4406089&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21262550%26dopt%3DAbstract Authors: Golberg D, Kroupitski Y, Belausov E, Pinto R, Sela S Despite washing and decontamination, outbreaks linked to consumption of fresh or minimally-processed leafy greens have been increasingly reported in recent years. In order to assure the safety of produce it is necessary to gain knowledge regarding the exact routes of contamination. Leaf internalization through stomata was previously reported as a potential route of contamination, which renders food-borne pathogens protected from washing and disinfection by sanitizers. In the present study we have examined the incidence (percentage of microscopic fields harboring ≥1 GFP-tagged bacteria) of Salmonella Typhimurium on the surface and underneath the epidermis in detached leaves of seven vegetables and fresh herbs. The incidence... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4406089 Sat, 08 Jan 2011 00:00:00 +0100 4406089 http://www.medworm.com/index.php?rid=4406085&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21262551%26dopt%3DAbstract Authors: Zhang Q, Feng Y, Deng L, Feng F, Wang L, Zhou Q, Luo Q The ability of Listeria monocytogenes to tolerate high levels of bile stress is critical to its successful infection and colonization in the human gastrointestinal tract. L. monocytogenes encodes bile salt hydrolase by a bsh gene which plays a significant role in hydrolyzing high concentrations of bile salt when L. monocytogenes grows under hypoxemic condition. As the bsh promoter contains consensus SigB and PrfA binding sites, we investigated the role of SigB (σ(B)) and PrfA in L. monocytogenes tolerance against bile stress by comparing the survival of isogenic deletion mutants of L. monocytogenes EGD(ΔsigB), EGD(ΔprfA) and EGD(ΔprfAΔsigB) with their parent strain EGD at high levels of bile salt. Our results show tha... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4406085 Sat, 08 Jan 2011 00:00:00 +0100 4406085 http://www.medworm.com/index.php?rid=4277554&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21144610%26dopt%3DAbstract Authors: Golowczyc MA, Silva J, Teixeira P, De Antoni GL, Abraham AG The injuries caused by spray drying (SD) of three potential probiotic lactobacilli isolated from kefir grains and the impact on some probiotic properties, were evaluated. Results demonstrated that Lactobacillus plantarum 83114 and L. kefir 8321 showed a slight reduction of viability (0.11 and 0.29logCFU/ml respectively) after SD process, and L. kefir 8348 was found to be more sensitive to the process with a reduction in viability of 0.70logCFU/ml. Neither membrane damage, evaluated by increased sensitivity to NaCl, lysozyme, bile salt and penicillin G, nor changes in acidifying activity in MRS and milk by lactobacilli were detected after SD. L. plantarum 83114 and L. kefir 8321 after SD did not lose their capacity to ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4277554 Wed, 22 Dec 2010 04:07:17 +0100 4277554 http://www.medworm.com/index.php?rid=4277552&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21145611%26dopt%3DAbstract Authors: Rocha LD, Reis GM, da Silva VN, Braghini R, Teixeira MM, Corrêa B Gibberella moniliformis is most commonly associated with maize worldwide and produces high levels of fumonisins, some of the most agriculturally important mycotoxins. Studies demonstrate that molecular methods can be helpful for a rapid identification of Fusarium species and their levels of toxin production. The purpose of this research was to apply molecular methods (AFLP, TEF-1α partial gene sequencing and PCR based on MAT alleles) for the identification of Fusarium species isolated from Brazilian corn and to verify if real time RT-PCR technique based on FUM1 and FUM19 genes is appropriated to estimate fumonisins B(1) and B(2) production levels. Among the isolated strains, 96 were identified as Fusarium vert... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4277552 Wed, 08 Dec 2010 00:00:00 +0100 4277552 http://www.medworm.com/index.php?rid=4251429&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21122933%26dopt%3DAbstract Authors: Abriouel H, Benomar N, Lucas R, Gálvez A Aloreña table olives are naturally fermented traditional green olives with a denomination of protection (DOP). The present study focused on Aloreña table olives manufactured by small and medium enterprises (SMEs) from Valle del Guadalhorce (Southern Spain) under three different conditions (cold storage, and ambient temperature fermentations in small vats and in large fermentation tanks). The microbial load of brines during fermentation was studied by plate counting, and the microbial diversity was determined by a culture-independent approach based on PCR-DGGE analysis. The viable microbial populations (total mesophilic counts, yeasts and molds, and lactic acid bacteria - LAB) changed in cell numbers during the course of fermentation.... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4251429 Mon, 29 Nov 2010 00:00:00 +0100 4251429 http://www.medworm.com/index.php?rid=4251428&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21138783%26dopt%3DAbstract Authors: Ottaviani D, Parlani C, Citterio B, Masini L, Leoni F, Canonico C, Sabatini L, Bruscolini F, Pianetti A The distribution of virulence properties in 142 strains of Aeromonas isolated from diarrhoeic patients, food and surface water in Italy and identified by biochemical and molecular methods was investigated. The virulence properties studied were the presence of genes for the aerolysin (aerA), heat-stable cytotonic enterotoxin (ast), heat-labile cytotonic enterotoxin (alt), cytotoxic enterotoxin (act); and cytotoxicity for Vero cells and adhesion on Hep-2 cells. A. hydrophila and A. caviae were the species most commonly isolated from clinical and environmental samples (9/30; 30.0% and 5/27; 18.5%, respectively) while mesophilic A. salmonicida was most common in food samples (19... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4251428 Wed, 17 Nov 2010 00:00:00 +0100 4251428 http://www.medworm.com/index.php?rid=4219193&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21093087%26dopt%3DAbstract Authors: Nieminen TT, Vihavainen E, Paloranta A, Lehto J, Paulin L, Auvinen P, Solismaa M, Björkroth KJ Characterization of psychrotrophic lactic acid bacteria (LAB) and Brochothrix thermosphacta communities is needed to understand the microbial ecology of spoilage of modified atmosphere-packed (MAP) meats. To overcome the limitations of the currently used methods for the characterization of psychrotrophic bacterial communities in meat, we developed a culture-independent, 16S rRNA gene-targeted terminal restriction fragment length polymorphism (T-RFLP) method. An identification library consisting of 100 Gram-positive and 30 Gram-negative meat-associated bacterial strains was set up to identify the terminal restriction fragments derived from the communities. The taxonomic resolution le... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4219193 Wed, 17 Nov 2010 00:00:00 +0100 4219193 http://www.medworm.com/index.php?rid=4185869&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21084126%26dopt%3DAbstract Authors: Sakamoto N, Tanaka S, Sonomoto K, Nakayama J Nukadoko is a naturally fermented rice bran mash traditionally used for pickling vegetables in Japan; its refreshment and fermentation cycles sometimes continue for many years. Here, we investigated the structure and dynamics of the bacterial community in nukadoko by conducting pyrosequencing and quantitative polymerase chain reaction (PCR) analyses of 16S ribosomal RNA genes (rDNA). Of the 16 different samples studied, 13 showed Lactobacillus-dominated microbiota, suggesting that aged nukadoko samples tend to realize a niche, favorable Lactobacillus species. The lactic acid bacterial community of each of the 16 samples was classified into 3 types according to the presence or absence of 2 predominant species, Lactobacillus namurensi... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185869 Mon, 15 Nov 2010 00:00:00 +0100 4185869 http://www.medworm.com/index.php?rid=4185870&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21078530%26dopt%3DAbstract Authors: Salazar N, Binetti A, Gueimonde M, Alonso A, Garrido P, González Del Rey C, González C, Ruas-Madiedo P, de Los Reyes-Gavilán CG Bifidobacterium animalis subsp. lactis IPLA R1 and Bifidobacterium longum IPLA E44 strains were tested for their safety and ability to modulate the intestinal microbiota in vivo. Chemically simulated gastrointestinal digestion showed considerably lower survival of E44 than R1 strain, the first microorganism also being more sensitive to refrigerated storage in 10% skimmed milk at 4°C. Harmful glycosidic activities were absent, or at low levels, in the strains R1 and E44. Both strains were sensitive to most antibiotics and resistant to aminoglycosides, a common feature in bifidobacteria. Similar to several other bifidobacteria strains, B. animalis s... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185870 Sat, 13 Nov 2010 00:00:00 +0100 4185870 http://www.medworm.com/index.php?rid=4185871&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21075469%26dopt%3DAbstract In this study, a total of 199 isolates of this species obtained from aquatic products (n=462) in Guangzhou City, China, were examined for their susceptibility to 19 antimicrobial agents and the presence of antimicrobial resistance integrons. The genetic relatedness of the isolates with integrons was also evaluated. A PCR-based method was used to screen integrons and found that 13 (6.5%) of the isolates harbored class 1 integrons. The antimicrobial resistance rates of integron-positive isolates were significantly higher than integron-negative ones for cefepime, ciprofloxacin, gentamicin, tetracycline, trimethoprim-sulfamethoxazole and rifampicin. Genetic sequence analysis revealed that these integrons contained various antimicrobial-resistance genes (dfrA1, dfrA14, dfrA17, dfrA32, aadA1, aa... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185871 Fri, 12 Nov 2010 00:00:00 +0100 4185871 http://www.medworm.com/index.php?rid=4185872&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21074885%26dopt%3DAbstract Authors: Yan H, Neogi SB, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L, Zhong N A total of 2177 food samples collected from nine cities in northern China during 2005 to 2007 were screened for the presence of Listeria monocytogenes. All L. monocytogenes isolates were subjected to serotyping, antimicrobial susceptibility, pulsed-field gel electrophoresis (PFGE), as well as PCR screening to identify genes responsible for tetracycline resistance [tet(L), tet(M), tet(K), tet(S) and tet(B)], transposon Tn916, and class 1 integron. Contamination with L. monocytogenes was detected in 4.13% (90/2177) of the total samples representing various food products. The pathogen was mainly isolated from frozen food made of wheat flour or rice products (26/252, 10.32%) and raw meat products (46/... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185872 Thu, 11 Nov 2010 00:00:00 +0100 4185872 http://www.medworm.com/index.php?rid=4185868&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21084128%26dopt%3DAbstract Authors: van der Veen S, Abee T We investigated the formation of single and mixed species biofilms of Listeria monocytogenes strains EGD-e and LR-991, with Lactobacillus plantarum WCFS1 as secondary species, and their resistance to the disinfectants benzalkonium chloride and peracetic acid. Modulation of growth, biofilm formation, and biofilm composition was achieved by addition of manganese sulfate and/or glucose to the BHI medium. Composition analyses of the mixed species biofilms using plate counts and fluorescence microscopy with dual fluorophores showed that mixed species biofilms were formed in BHI (total count, 8-9 log(10) cfu/well) and that they contained 1-2 log(10) cfu/well more L. monocytogenes than L. plantarum cells. Addition of manganese sulfate resulted in equal numbers ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185868 Fri, 29 Oct 2010 00:00:00 +0100 4185868 http://www.medworm.com/index.php?rid=4185867&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21084129%26dopt%3DAbstract Authors: Garcia D, Ramos AJ, Sanchis V, Marín S The aim of this study was to assess the impact of suboptimal environmental conditions on the intraspecific variability of A. carbonarius growth and OTA production using thirty isolates of A. carbonarius. Three a(w)/temperature conditions were tested, one optimal (0.98a(w)/25°C) and two suboptimal: 0.90a(w)/25°C and 0.98a(w)/37°C as suboptimal water activity and temperature, respectively, which might take place through over ripening and dehydration of grapes. For each condition, 12 Petri dishes were inoculated, and colony growth and OTA production were measured over time. ANOVA revealed significant differences among μ and λ within the 30 assayed isolates. Coefficients of variation (CV%) revealed a wider dispersion of growth rates at ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4185867 Fri, 29 Oct 2010 00:00:00 +0100 4185867 http://www.medworm.com/index.php?rid=4139709&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036411%26dopt%3DAbstract Authors: Teunis PF, Kasuga F, Fazil A, Ogden ID, Rotariu O, Strachan NJ Salmonella is a key human pathogen worldwide, most often associated with food poisoning incidences. There is a small number of predominant serotypes found in human cases. The role of exposure in the epidemiology of Salmonella can be explained using dose-response assessment both for infection and acute enteric illness. Dose-response studies are traditionally based on human challenge experiments but an alternative is to use outbreak data. Such data were collected from the published literature which included estimates of the dose ingested and the attack rate. Separate dose-response models for infection and illness given infection were fitted using a multi-level statistical framework. These models incorporated serotype... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4139709 Fri, 29 Oct 2010 00:00:00 +0100 4139709 http://www.medworm.com/index.php?rid=4139710&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21035884%26dopt%3DAbstract The objective of this study was to address knowledge gaps identified in an earlier risk assessment of Staphylococcus aureus and raw milk cheese. A survey of fresh and short-time ripened cheeses produced on farm-dairies in Sweden was conducted to investigate the occurrence and levels of S. aureus, Listeria monocytogenes and Escherichia coli, to characterize S. aureus isolates with special emphasis on enterotoxin genes, antibiotic resistance, bio-typing and genetic variation, and to collect information related to production practices. In general, the hygienic quality of farm-dairy cheeses appeared to be of an acceptable microbiological quality, e.g. L. monocytogenes and staphylococcal enterotoxin were not detected in cheese samples. However, E. coli and enterotoxigenic S. aureus were frequen... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4139710 Thu, 28 Oct 2010 00:00:00 +0100 4139710 http://www.medworm.com/index.php?rid=4139711&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21035223%26dopt%3DAbstract This study will be the basis for further investigations to identify and exploit the basis of diversity in the stress response of lactic acid bacteria. PMID: 21035223 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4139711 Wed, 27 Oct 2010 00:00:00 +0100 4139711 http://www.medworm.com/index.php?rid=4088527&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20951455%26dopt%3DAbstract Authors: Kong M, Chen XG, Xing K, Park HJ Owing to its high biodegradability, and nontoxicity and antimicrobial properties, chitosan is widely-used as an antimicrobial agent either alone or blended with other natural polymers. To broaden chitosan's antimicrobial applicability, comprehensive knowledge of its activity is necessary. The paper reviews the current trend of investigation on antimicrobial activities of chitosan and its mode of action. Chitosan-mediated inhibition is affected by several factors can be classified into four types as intrinsic, environmental, microorganism and physical state, according to their respective roles. In this review, different physical states are comparatively discussed. Mode of antimicrobial action is discussed in parts of the active compound (chitosa... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4088527 Wed, 13 Oct 2010 23:00:00 +0100 4088527 http://www.medworm.com/index.php?rid=4063197&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20937537%26dopt%3DAbstract This study was aimed to use Fourier Transform Infrared (FTIR) spectroscopy integrated with an attenuated total reflectance kit for quantification of Escherichia coli K-12 internalized in baby spinach. The bacteria were inoculated into vascular and intracellar tissues of spinach leaves by syringe injection and the distribution of internalized E. coli K-12 cells was confirmed under scanning electron microscopy (SEM). FTIR measurement following the preparation of bacterial suspension from spinach leaves with high speed pulverizing enabled to detect the absorbance peaks in the amide II region between 1590 and 1490cm(-1) as a fingerprint for the microbes. It was found that the estimated concentrations of E. coli K-12 agreed well with the concentrations determined by plate counting with R(2) val... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4063197 Fri, 08 Oct 2010 23:00:00 +0100 4063197 http://www.medworm.com/index.php?rid=4063198&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20926150%26dopt%3DAbstract Authors: Chang SS, Redondo-Solano M, Thippareddi H Alfalfa and other seed sprouts have been implicated in several Escherichia coli O157:H7 and Salmonella spp. human illness outbreaks in the U.S. Continuing food safety issues with alfalfa seeds necessitate the need for discovery and use of novel and effective antimicrobials. The potential use of caprylic acid (CA) and monocaprylin (MC) for reducing E. coli O157:H7 and Salmonella spp. populations on alfalfa seeds was evaluated. The effectiveness of three concentrations of CA and MC (25, 50, and 75mM) to reduce E. coli O157:H7 and Salmonella spp. populations in 0.1% peptone water and on alfalfa seeds was evaluated. Surviving populations of E. coli O157:H7 and Salmonella spp. were enumerated by direct plating on tryptic soy agar (TSA). Non... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4063198 Sun, 03 Oct 2010 23:00:00 +0100 4063198 http://www.medworm.com/index.php?rid=4044285&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20888656%26dopt%3DAbstract Authors: Mayrhofer S, van Hoek AH, Mair C, Huys G, Aarts HJ, Kneifel W, Domig KJ The range of antibiotic susceptibility to 13 antibiotics in 101 strains of the Lactobacillus acidophilus group was examined using the lactic acid bacteria susceptibility test medium (LSM) and broth microdilution. Additionally, microarray analysis and PCR were applied to identify resistance genes responsible for the displayed resistant phenotypes in a selection of strains. In general, narrow as well as broad unimodal and bimodal MIC distributions were observed for the Lactobacillus acidophilus group and the tested antimicrobial agents. Atypically resistant strains could be determined by visual inspection of the obtained MIC ranges for ampicillin, chloramphenicol, clindamycin, erythromycin, quinupristin/dalf... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4044285 Wed, 29 Sep 2010 23:00:00 +0100 4044285 http://www.medworm.com/index.php?rid=4003962&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20864199%26dopt%3DAbstract This study examines the depuration of oysters (Crassostrea virginica) and hard shell clams (Mercinaria mercinaria) in a flow-through depuration system under variable temperature (12°C, 18°C, and 25°C), salinity (8ppt, 18ppt, and 28ppt), turbidity (<1NTU, 10NTU, and 20NTU), pH (pH 7 and pH 8), and algae conditions (0 cells/mL and 50,000 cells/mL), with constant dissolved oxygen (5-7mg/L). Oysters and hard shell clams were artificially contaminated with enteric microorganisms: Escherichia coli, Enterococcus faecalis, coliphage MS2, Poliovirus type-1 and Hepatitis A virus HM-175 (HAV), then depurated in 5-day trials with daily sampling. In oysters, optimizing environmental parameters of water temperature improved E. coli, MS2, poliovirus and HAV depuration, and optimized salinity ... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4003962 Mon, 20 Sep 2010 23:00:00 +0100 4003962 http://www.medworm.com/index.php?rid=4003901&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20864200%26dopt%3DAbstract In this study, fungi differed in their response to modified atmospheres in biomass, ergosterol content, mycotoxin production and morphology. Reductions of 57.8-96.9%, 73.7-99.6% and 91.5-99.9% were obtained in colony diameter, hyphal length and ergosterol content, respectively, under this atmosphere compared to air. Ergosterol content was more affected in most species than other measurements. Patulin, cyclopiazonic acid and roquefortine C were produced in this atmosphere, although levels were very low and aflatoxin was not produced at all. Growth was quite extensive as measured by colony diameters, but hyphal lengths were low and ergosterol production was also affected in all species of this study. PMID: 20864200 [PubMed - as supplied by publisher] (Source: International Journal of Foo... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4003901 Mon, 20 Sep 2010 23:00:00 +0100 4003901 http://www.medworm.com/index.php?rid=4003819&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20864201%26dopt%3DAbstract Authors: Wood JD, Bezanson GS, Gordon RJ, Jamieson R Recent outbreaks of food-borne illnesses associated with the consumption of fresh produce have increased attention on irrigation water as a potential source of pathogen contamination. A better understanding of the behaviour of enteric pathogens introduced into agricultural systems during irrigation will aid in risk assessments and support the development of appropriate farm-level water management practices. For this reason, the survival dynamics of two nalidixic acid resistant strains of Escherichia coli after their spray inoculation into the phyllosphere and soil of field spinach were examined over two growing seasons. E. coli strains NAR, an environmental isolate, and DM3n, a non-pathogenic serotype O157:H7, were applied at rates o... International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4003819 Mon, 20 Sep 2010 23:00:00 +0100 4003819 http://www.medworm.com/index.php?rid=4004073&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20858570%26dopt%3DAbstract Authors: Buzrul S PMID: 20858570 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4004073 Sun, 19 Sep 2010 23:00:00 +0100 4004073 http://www.medworm.com/index.php?rid=4004021&cid=s_35639_143_f&fid=35639&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20863585%26dopt%3DAbstract Rebuttal: Response to regarding letter to editor "On the modeling of inactivation kinetics by UV irradiation" Int J Food Microbiol. 2010 Sep 20; Authors: Unluturk S PMID: 20863585 [PubMed - as supplied by publisher] (Source: International Journal of Food Microbiology) International Journal of Food Microbiology journals http://www.medworm.com/rss/comments.php?id=4004021 Sun, 19 Sep 2010 23:00:00 +0100 4004021