MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Laboratory Hematology' source. Wed, 08 Feb 2012 17:42:03 +0100 http://www.medworm.com/index.php?rid=5538493&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188998%26dopt%3DAbstract Authors: PMID: 22188998 [PubMed - in process] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5538493 Sun, 25 Dec 2011 09:06:13 +0100 5538493 http://www.medworm.com/index.php?rid=5538494&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22168550%26dopt%3DAbstract Conclusion:  The results indicate that the risk of acute leukemia might be associated with JAK2 polymorphisms. PMID: 22168550 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5538494 Wed, 14 Dec 2011 05:00:00 +0100 5538494 http://www.medworm.com/index.php?rid=5538495&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22151199%26dopt%3DAbstract Conclusions:  The new RBC parameters of CELL-DYN Sapphire generally correlated well with those of Advia 120, although significant systematic differences were present, particularly in reticulocyte MCH and MCV. These differences necessitate instrument-specific reference ranges and clinical decision values. To minimize preanalytical effects, these parameters should be measured in fresh blood samples. PMID: 22151199 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5538495 Thu, 08 Dec 2011 05:00:00 +0100 5538495 http://www.medworm.com/index.php?rid=5496135&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22145750%26dopt%3DAbstract Conclusion:  Bortezomib is a promising potential therapy for acute leukemia, especially mdr1 drug-resistant leukemia. PMID: 22145750 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5496135 Wed, 07 Dec 2011 05:00:00 +0100 5496135 http://www.medworm.com/index.php?rid=5496134&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22145778%26dopt%3DAbstract Conclusions:  The crush technique seems to be more valuable than the wedge-spread technique because of the lack of a blood dilution effect and better assessment of megakaryopoiesis. We recommend the crush technique for the evaluation of the percentage composition of bone marrow cells. In a very small number of patients with irregular cell localization in the bone marrow particles, the wedge-spread technique may be more beneficial for the assessment of total cellularity. The recommendation to routinely prepare slides using both of these techniques is fully justified. PMID: 22145778 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5496134 Wed, 07 Dec 2011 05:00:00 +0100 5496134 http://www.medworm.com/index.php?rid=5496131&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22145801%26dopt%3DAbstract Conclusion:  A group of proteins associated with BC can be obtained and the result of this study might provide clues for further research. PMID: 22145801 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5496131 Wed, 07 Dec 2011 05:00:00 +0100 5496131 http://www.medworm.com/index.php?rid=5496136&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22122800%26dopt%3DAbstract We describe here a rare chromosomal rearrangement, t(14;19)(q11.2;q13.3), in a Lennert's lymphoma, a variant of PTCL, not otherwise specified. Sequential fluorescence in situ hybridization assays showed that the breakpoint in 19q13.3 was located distal to the BCL3 and PVRL2 genes, both of which may be candidate proto-oncogenes. These findings suggest that another gene is involved in the pathogenic characteristics observed in this patient with Lennert's lymphoma. PMID: 22122800 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5496136 Tue, 29 Nov 2011 05:00:00 +0100 5496136 http://www.medworm.com/index.php?rid=5427935&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22085261%26dopt%3DAbstract Conclusion:  In this collective of subjectively healthy elderly individuals, monocyte anisocytosis, neutrophil anisocytosis and mean lymphocyte volume were associated with decreased HoloTC. PMID: 22085261 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5427935 Wed, 16 Nov 2011 05:00:00 +0100 5427935 http://www.medworm.com/index.php?rid=5427936&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081912%26dopt%3DAbstract Authors: Zini G, d'Onofrio G, Briggs C, Erber W, Jou JM, Lee SH, McFadden S, Vives-Corrons JL, Yutaka N, Lesesve JF Abstract Schistocytes are fragments of red blood cells (RBCs) produced by extrinsic mechanical damage within the circulation. The detection of schistocytes is an important morphological clue to the diagnosis of thrombotic microangiopathic anemia (TMA). Reporting criteria between different laboratories, however, are not uniform, owing to variability of shape and nature of fragments, as well as subjectivity and heterogeneity in their morphological assessment. Lack of standardization may lead to inconsistency or misdiagnosis, thereby affecting treatment and clinical outcome. The Schistocyte Working Group of the International Council for Standardization in Haematology (IC... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5427936 Tue, 15 Nov 2011 05:00:00 +0100 5427936 http://www.medworm.com/index.php?rid=5427937&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22078096%26dopt%3DAbstract Conclusion:  Determination of P5'N-1 activity by using an ELISA reader is a new, simple, less time consuming and reliable method. It also avoids the use of radioactive material or HPLC which is a significant advantage. PMID: 22078096 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5427937 Mon, 14 Nov 2011 05:00:00 +0100 5427937 http://www.medworm.com/index.php?rid=5427938&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22074115%26dopt%3DAbstract Conclusion:  DxH800â„¢ provides very sensitive and specific, easily recognizable P. vivax malaria signals on routine CBC without need for the additional reagents or special procedures. PMID: 22074115 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5427938 Fri, 11 Nov 2011 05:00:00 +0100 5427938 http://www.medworm.com/index.php?rid=5427939&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22067299%26dopt%3DAbstract Conclusion:  We suggest that in healthy individuals, a typical smear would contain several nondiscoid-shaped erythrocytes, only clearly visible at high magnification. However, thromboembolic ischemic stroke does significantly impact erythorcyte shape, and this change in morphology may result in an impaired microcirculation, as well as impaired oxygen carrying capacity. This changed morphology may further complicate the restoring of homeostasis caused by acute thromboembolic stroke. PMID: 22067299 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5427939 Wed, 09 Nov 2011 05:00:00 +0100 5427939 http://www.medworm.com/index.php?rid=5383295&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22051137%26dopt%3DAbstract Conclusion:  The results of routine hematological testing on mildly to frankly hemolyzed specimens might be unreliable. PMID: 22051137 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5383295 Thu, 03 Nov 2011 04:00:00 +0100 5383295 http://www.medworm.com/index.php?rid=5383298&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22032515%26dopt%3DAbstract Conclusion:  The combination of mixing and confirmatory tests interpreted according to the new guidelines can clearly differentiate LA from other coagulopathies. PMID: 22032515 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5383298 Fri, 28 Oct 2011 04:00:00 +0100 5383298 http://www.medworm.com/index.php?rid=5317096&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21985133%26dopt%3DAbstract Conclusion:  The decrease in blood catalase activity might be due to the damaging effects of free radicals on the catalase protein. Consequently, these beta-thalassemia trait patients may be relatively susceptible to damage caused by oxidative stress. PMID: 21985133 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5317096 Mon, 10 Oct 2011 04:00:00 +0100 5317096 http://www.medworm.com/index.php?rid=5302012&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21974826%26dopt%3DAbstract Authors: Stephens AD, Angastiniotis M, Baysal E, Chan V, Fucharoen S, Giordano PC, Hoyer JD, Mosca A, Wild B, Abstract Although DNA analysis is needed for characterization of the mutations that cause β-thalassaemia, measurement of the Hb A(2) is essential for the routine identification of people who are carriers of β-thalassaemia. The methods of quantitating Hb A(2) are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high-performance liquid chromatography and capillary electrophoresis. PMID: 21974826 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5302012 Wed, 05 Oct 2011 04:00:00 +0100 5302012 http://www.medworm.com/index.php?rid=5195558&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884505%26dopt%3DAbstract Conclusion:  The NaOH method is a simple method that uses minimal equipment and reagents that make it labour- and cost-effective. This method could be adopted by poorer countries to extract DNA for beta-thalassaemia mutation characterization. PMID: 21884505 [PubMed - in process] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195558 Tue, 06 Sep 2011 02:56:12 +0100 5195558 http://www.medworm.com/index.php?rid=5195563&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883966%26dopt%3DAbstract Authors: Bench AJ Abstract The identification of the molecular genetic basis to many haematological malignancies along with the increased use of molecularly targeted therapy has heralded an increasing role for molecular genetic-based techniques. Demonstration of acquired changes such as the JAK2 V617F mutation within myeloproliferative neoplasms has quickly moved from a research setting to the diagnostic laboratory. Disease-specific genetic markers, such as the BCR-ABL1 fusion gene in chronic myeloid leukaemia, enable sensitive molecular genetic methods to be applied for the detection and quantification of low-level residual disease, allowing early identification of relapse. Consequently, molecular genetics now plays a crucial role in diagnosis, the identification of prognostic mar... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195563 Thu, 25 Aug 2011 23:00:00 +0100 5195563 http://www.medworm.com/index.php?rid=5195559&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883970%26dopt%3DAbstract Conclusion:  CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70 fL), this approach provided a high resolution to identify Hb H-CS disease. PMID: 21883970 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195559 Thu, 25 Aug 2011 23:00:00 +0100 5195559 http://www.medworm.com/index.php?rid=5195562&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883967%26dopt%3DAbstract Conclusion:  A minimal panel of immunohistochemical markers should include anti-CD43 or anti-lysozyme as a lack of immunoreactivity for either of these sensitive markers would be inconsistent with a diagnosis of myeloid sarcoma. Use of more specific markers of myeloid disease, such as CD33, myeloperoxidase, CD34 and CD117 is necessary to establish the diagnosis. Other antibodies may be added depending on the differential diagnosis. Identification of acute myeloid leukemia-associated genetic lesions may be helpful in arriving at the correct diagnosis. PMID: 21883967 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195562 Tue, 23 Aug 2011 23:00:00 +0100 5195562 http://www.medworm.com/index.php?rid=5195561&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883968%26dopt%3DAbstract Conclusion:  We concluded that in the presence of a normal WBC and haemoglobin, the FBC is normal in almost all cases and measuring these two parameters could be used as an effective screen to predict FBC normality. PMID: 21883968 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195561 Tue, 23 Aug 2011 23:00:00 +0100 5195561 http://www.medworm.com/index.php?rid=5195560&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883969%26dopt%3DAbstract Conclusion:  Alterations of proteins, lipids, and ions in the plasma and erythrocyte of steady-state patients with SCA were demonstrated. Some of these alterations are related with each other and with the oxidative stress observed in the disease. PMID: 21883969 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195560 Tue, 23 Aug 2011 23:00:00 +0100 5195560 http://www.medworm.com/index.php?rid=5195564&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883965%26dopt%3DAbstract Conclusion:  The described novel test tube method could be an alternative method of mass population screening for HbE, particularly in small health care facilities. PMID: 21883965 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5195564 Mon, 22 Aug 2011 23:00:00 +0100 5195564 http://www.medworm.com/index.php?rid=5142751&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21834798%26dopt%3DAbstract Conclusion:  Measurement of Ca-I-activated CL enhanced with 10(-4)  m luminol is recommended for the detection of intracellular ROS. The addition of HRP leads to the detection of overall ROS production while the OZP-activated system with its addition of HRP can only be used to detect overall ROS production. Ca-I-activated CL enhanced with 10(-4)  m isoluminol and with addition of HRP is recommended for the detection of extracellular CL. PMID: 21834798 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=5142751 Wed, 10 Aug 2011 23:00:00 +0100 5142751 http://www.medworm.com/index.php?rid=4971558&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692994%26dopt%3DAbstract Authors: Baglin T Characterisation of heritable thrombophilic defects has facilitated an understanding of the complex mechanisms influencing risk of venous thromboembolism. In parallel with this, the importance of gene-environment interaction in the development of this disease has become apparent. However, testing for a limited number of heritable thrombophilic defects (first generation thrombophilia testing) has not been shown to predict likelihood of recurrent venous thrombosis to any useful degree. This paradox whereby thrombophilia testing identifies defects associated with an increased risk of a first venous thrombosis but not of a particularly high risk of recurrence is likely the result of limitations imposed by a limited dichotomous testing strategy compounded by test inaccurac... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971558 Mon, 27 Jun 2011 22:31:10 +0100 4971558 http://www.medworm.com/index.php?rid=4971557&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692995%26dopt%3DAbstract Conclusion:  East Asian DS neonates and children showed distinctive spectrum of hematological abnormalities. PMID: 21692995 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971557 Mon, 27 Jun 2011 22:31:00 +0100 4971557 http://www.medworm.com/index.php?rid=4971556&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692996%26dopt%3DAbstract Conclusion:  The present study revealed an association of pDCs, mDCs, γδT cells and Treg cells with induction or treatment of GVHD. PMID: 21692996 [PubMed - in process] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971556 Mon, 27 Jun 2011 22:30:45 +0100 4971556 http://www.medworm.com/index.php?rid=4971555&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692997%26dopt%3DAbstract Authors: McCarron SL, Haslam K, Kelly J, Duggan C, Langabeer SE PMID: 21692997 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971555 Tue, 21 Jun 2011 23:00:00 +0100 4971555 http://www.medworm.com/index.php?rid=4971554&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692998%26dopt%3DAbstract This study aimed to quantify unnecessary repeat haematinic tests taken from the elderly in a district general hospital. Haematinic tests (ferritin, B12, serum folate) from patients age ≥70 years were reviewed for repeat tests during an 8-week period. Questionnaires were given to doctors to establish when the considered repeating a 'borderline low normal' result to be clinically justifiable. 7.7% of all haematinic tests were repeat tests and of these, the majority (83%) was performed following a previously normal result. Thirteen of 24 doctors believed repeating a normal result at the bottom of the normal range ('borderline low normal') was justifiable. After excluding 'borderline low normal' results, 6.0% (at minimum) of repeat tests were done following a previous normal result and wer... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971554 Tue, 21 Jun 2011 23:00:00 +0100 4971554 http://www.medworm.com/index.php?rid=4971562&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679309%26dopt%3DAbstract Conclusion: ; Critical control of the factors influencing the LS1+ flag can significantly decrease the number of microscopic samples to be reviewed and may be valuable for every laboratory performing routine differentials, using any type of hematology analyzer. PMID: 21679309 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971562 Wed, 15 Jun 2011 23:00:00 +0100 4971562 http://www.medworm.com/index.php?rid=4971561&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679310%26dopt%3DAbstract Authors: Cooper PC, Coath FL, Daly M, Makris M PMID: 21679310 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971561 Wed, 15 Jun 2011 23:00:00 +0100 4971561 http://www.medworm.com/index.php?rid=4971560&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679311%26dopt%3DAbstract Authors: Li WJ, Cui L, Gao C, Zhao XX, Liu SG, Xing YP, Zhang RD, Zhang DW, Wang B, Li ZG, Wu MY PMID: 21679311 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971560 Wed, 15 Jun 2011 23:00:00 +0100 4971560 http://www.medworm.com/index.php?rid=4971559&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679312%26dopt%3DAbstract Authors: Marlar RA PMID: 21679312 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971559 Wed, 15 Jun 2011 23:00:00 +0100 4971559 http://www.medworm.com/index.php?rid=4971565&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21668655%26dopt%3DAbstract Conclusion:  Our study revealed a lower mean CV for the total cell count for the XE-5000 method. The fully automated CSF cell count results in a 7.5-fold reduction in TAT and leads to a significant decrease in total analytical performance costs. PMID: 21668655 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971565 Sun, 12 Jun 2011 23:00:00 +0100 4971565 http://www.medworm.com/index.php?rid=4971564&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21668656%26dopt%3DAbstract Authors: Favaloro EJ PMID: 21668656 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971564 Sun, 12 Jun 2011 23:00:00 +0100 4971564 http://www.medworm.com/index.php?rid=4971563&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21668657%26dopt%3DAbstract Conclusion:  The single closed-tube T(m) -shift method for HPA-15 genotyping is high-throughput, rapid, reliable, reproducible and cost-effective and it is superior to conventional PCR-SSP used in routine genotyping of HPA-15. PMID: 21668657 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4971563 Sun, 12 Jun 2011 23:00:00 +0100 4971563 http://www.medworm.com/index.php?rid=4923055&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21645281%26dopt%3DAbstract We describe a case of l-asparaginase-associated severe hyperlipidemia with complete blood count abnormalities. Complete blood count analysis was performed with Beckman COULTER(®) GEN·Sâ„¢ system, which uses the Coulter Volume, Conductivity, Scatter technology to probe hydrodynamically focused cells. Although an expected significant inaccuracy in hemoglobin determination occurred starting from a lipid value of 3450 mg/dl, we observed that triglyceride level was 1466 mg/dl. Complete blood count analysis revealed that exceptionally high hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels vs. discordant with red blood cell count, mean corpuscular volume, and hematocrit levels. Total leukocyte count altered spontaneously in a wide range, and was c... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4923055 Sun, 05 Jun 2011 23:00:00 +0100 4923055 http://www.medworm.com/index.php?rid=4923054&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21645282%26dopt%3DAbstract Authors: Fujita H, Hamaki T, Ohwada A, Tomiyama J, Nishimura S PMID: 21645282 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4923054 Sun, 05 Jun 2011 23:00:00 +0100 4923054 http://www.medworm.com/index.php?rid=4923053&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21645283%26dopt%3DAbstract Authors: Saki N, Saki F, Saki MR, Karimi M PMID: 21645283 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4923053 Sun, 05 Jun 2011 23:00:00 +0100 4923053 http://www.medworm.com/index.php?rid=4923057&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21631891%26dopt%3DAbstract Conclusion:  The results obtained indicate the UniCel DxH 800 to be an excellent test on neonatal blood and superior to the other analyzers. Therefore, the DxH 800 is an effective and highly sensitive system for the analysis of NRBCs on newborns. PMID: 21631891 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4923057 Tue, 31 May 2011 23:00:00 +0100 4923057 http://www.medworm.com/index.php?rid=4923056&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21631892%26dopt%3DAbstract Conclusion:  The method is valid for accurate quantification of Hb at a wide concentration range (>0.1 μm/L) in erythroid precursors or plasma and is optional for other biological fluids. PMID: 21631892 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4923056 Tue, 31 May 2011 23:00:00 +0100 4923056 http://www.medworm.com/index.php?rid=4877129&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21605346%26dopt%3DAbstract Conclusion:  Circulating ECs might be associated with systemic aGVHD. PMID: 21605346 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877129 Mon, 23 May 2011 23:00:00 +0100 4877129 http://www.medworm.com/index.php?rid=4877132&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21569219%26dopt%3DAbstract Authors: Davidson SJ, Woodhams B Antithrombin inhibits VIIa when bound to cellular tissue factor in the presence of heparin. VIIa concentrations increase within the surgical field during cardiopulmonary bypass surgery but decrease when measured in the patient. Using a new ELISA (Stago, Reading, UK), we measured VIIa-antithrombin complexes in patients undergoing cardiopulmonary bypass to determine whether antithrombin plays a physiological role in VIIa inhibition during cardiac surgery. Samples were taken from 13 adult patients undergoing cardiac surgery with cardiopulmonary bypass at the following time points: presurgery, postheparin, 20 min intervals during cardiopulmonary bypass and postprotamine. The presurgery concentrations of VIIa-antithrombin complexes were median of 52.7pm, a... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877132 Sun, 15 May 2011 23:00:00 +0100 4877132 http://www.medworm.com/index.php?rid=4877131&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21569220%26dopt%3DAbstract Conclusion:  To enhance the appropriate use of hereditary thrombophilia screening tests, physician education concerning the patient selection, suitable timing for testing and repetition of the tests with abnormal results should be emphasized. PMID: 21569220 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877131 Sun, 15 May 2011 23:00:00 +0100 4877131 http://www.medworm.com/index.php?rid=4877130&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21569221%26dopt%3DAbstract Conclusion:  We suggested using roller mixer to improve the reliability of coagulation testing. Such standardization in preanalytical phase may be helpful in preventing laboratory errors and obtaining correct test results in coagulation tests. PMID: 21569221 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877130 Sun, 15 May 2011 23:00:00 +0100 4877130 http://www.medworm.com/index.php?rid=4877133&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21545688%26dopt%3DAbstract Conclusion:  Sampling for pO(2) determination should be carried out in small volumes and assessed within 30 min of collection to obtain reliable and comparable results. PMID: 21545688 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877133 Thu, 05 May 2011 23:00:00 +0100 4877133 http://www.medworm.com/index.php?rid=4877136&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535419%26dopt%3DAbstract Conclusion:  The INNOVANCE Antithrombin assay has high sensitivity for Antithrombin deficiencies and is reliable, precise and suitable for routine clinical use. PMID: 21535419 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877136 Mon, 02 May 2011 23:00:00 +0100 4877136 http://www.medworm.com/index.php?rid=4877135&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535479%26dopt%3DAbstract Authors: Murray C, Hall SK, Griffiths P The West Midlands Newborn Screening Laboratory (NBSL) at Birmingham Children's Hospital (BCH), UK, screens approximately 71 000 babies per annum using the Bio-Rad automated VARIANTâ„¢ nbs (Vnbs) high-pressure liquid chromatograph (HPLC). Any abnormal haemoglobins detected, including S, C, D-Punjab, E and O-Arab as directed by the NHS Sickle Cell and Thalassaemia Screening Programme (NHS Sickle Cell and Thalassaemia Screening Programme Website, http://sct.screening.nhs.uk), are then confirmed using Resolve(®) isoelectric electric focusing (IEF) kits supplied by Perkin-Elmer. The Sebia capillarys Neonat Haemoglobin FASTâ„¢ system was evaluated as a possible replacement for the first- or second-line methods used. Both the Sebia and Bio-Rad method... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877135 Mon, 02 May 2011 23:00:00 +0100 4877135 http://www.medworm.com/index.php?rid=4877134&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535418%26dopt%3DAbstract Authors: PMID: 21535418 [PubMed - indexed for MEDLINE] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4877134 Sat, 30 Apr 2011 23:00:00 +0100 4877134 http://www.medworm.com/index.php?rid=4770322&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21507207%26dopt%3DAbstract Conclusion:  We concluded that 563C-T was the commonest G6PD variant, while 1003A-G and 131C-G were less-frequent genetic variants of G6PD in Pakistani population. A novel genetic variant 973G-A was also identified. Very low levels of G6PD enzyme activity was seen with G6PD 563C-T. Mutational analysis failed in a significant proportion of samples warranting further work. PMID: 21507207 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770322 Wed, 20 Apr 2011 23:00:00 +0100 4770322 http://www.medworm.com/index.php?rid=4770323&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21501392%26dopt%3DAbstract Conclusion:  Glucose-6-phosphate dehydrogenase heterozygote females cannot be identified by FST if fluorescence is reported as absent or present. Distinguishing samples with intermediate fluorescence from absent and bright fluorescence improves detection of heterozygote females with mild G6PD deficiency. Mutational studies confirmed that 85% of intermediate samples with normal enzyme activity had identifiable G6PD mutations. PMID: 21501392 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770323 Mon, 18 Apr 2011 23:00:00 +0100 4770323 http://www.medworm.com/index.php?rid=4770324&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21492411%26dopt%3DAbstract Conclusion:  This study demonstrates a new and efficient method to amplify the CD34(+) human cord blood hematopoietic stem/progenitor cells in a 3D alginate culture system ex vivo for extended periods while retaining the hematopoietic reconstruction capacity. PMID: 21492411 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770324 Wed, 13 Apr 2011 23:00:00 +0100 4770324 http://www.medworm.com/index.php?rid=4770327&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470371%26dopt%3DAbstract Conclusion:  Approximately 1/3 of PCM cases show IP changes over time, with CD45 the least stable antigen. Recognition of this relative instability is important to avoid narrow targeting of follow-up FC analyses, especially for minimal residual disease monitoring. PMID: 21470371 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770327 Wed, 06 Apr 2011 23:00:00 +0100 4770327 http://www.medworm.com/index.php?rid=4770326&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470372%26dopt%3DAbstract Conclusion:  Stanleyville-II gene mutation is HBA2:c.237C>A, or C>G, and this information on the Globin Gene Server should be updated; AfeI test is a fast and accurate method to detect it; NBS programs should consider the possibility of Hb Stanleyville-II whenever IEF shows one band in the HbS position, and another one between S and C. PMID: 21470372 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770326 Wed, 06 Apr 2011 23:00:00 +0100 4770326 http://www.medworm.com/index.php?rid=4770325&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470373%26dopt%3DAbstract Conclusion:  In PCN cases, an FC-assessed 3% PCC in BM aspirates should not be used as a cut-off for further FISH testing. PMID: 21470373 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770325 Wed, 06 Apr 2011 23:00:00 +0100 4770325 http://www.medworm.com/index.php?rid=4770328&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21463487%26dopt%3DAbstract Conclusion:  According to our data, patients with low producer type of IL-10 polymorphisms have more severe thrombocytopenia, suggesting that IL-10 gene polymorphisms may reflect the severity of ITP. PMID: 21463487 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770328 Mon, 04 Apr 2011 23:00:00 +0100 4770328 http://www.medworm.com/index.php?rid=4770333&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457188%26dopt%3DAbstract Conclusion:  Our data suggest that ZnPcH(1) -PDT may be a useful modality for purging CML cells for autologous grafts. PMID: 21457188 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770333 Sun, 03 Apr 2011 23:00:00 +0100 4770333 http://www.medworm.com/index.php?rid=4770332&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457189%26dopt%3DAbstract Authors: Hänscheid T, Romão R, Grobusch MP, Amaral T, Melo-Cristino J PMID: 21457189 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770332 Sun, 03 Apr 2011 23:00:00 +0100 4770332 http://www.medworm.com/index.php?rid=4770331&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457190%26dopt%3DAbstract Authors: Haslam K, Kelly J, Morris T, Connaghan G, Gilligan O, Browne P, Langabeer SE PMID: 21457190 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4770331 Sun, 03 Apr 2011 23:00:00 +0100 4770331 http://www.medworm.com/index.php?rid=4607236&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21382180%26dopt%3DAbstract Authors: Inati A, Musallam KM, Cappellini MD, Duca L, Taher AT The value of nontransferrin-bound iron (NTBI) as an index of iron overload in patients with thalassemia has been evaluated; however, data in patients with sickle cell disease (SCD) is limited. NTBI levels were evaluated in a cross-sectional study of 43 transfused patients with SCD. Patient charts were reviewed for demographics, status of the spleen, and total number of lifetime transfusions. All patients were chelation naïve and none of the patients had evidence of hepatitis B or C infection. Blood samples were taken for assessment of NTBI and serum ferritin (SF); liver iron concentration (LIC) was determined by R2 magnetic resonance imaging. NTBI levels were generally low with a median of -0.01 μm (range -2.56 to 6.37â... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4607236 Fri, 18 Mar 2011 18:15:21 +0100 4607236 http://www.medworm.com/index.php?rid=4607235&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21401902%26dopt%3DAbstract Conclusion:  AT activity assays are essential for the detection of AT deficiency because type II defects are relatively common in patients with heritable deficiency. No one functional assay can be assumed to detect all forms of AT deficiency, and assays can sometimes be improved by reducing reaction time of AT with thrombin or factor Xa. PMID: 21401902 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4607235 Tue, 15 Mar 2011 00:00:00 +0100 4607235 http://www.medworm.com/index.php?rid=4545284&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21352508%26dopt%3DAbstract Authors: Jou JM, Lewis SM, Briggs C, Lee SH, DE LA Salle B, McFadden S, In recognition of the need for a standardization of the measurement of the erythrocyte sedimentation rate (ESR), the International Council for Standardization in Haematology makes the following recommendations: (i) The reference method for measurement of the ESR should be based on the Westergren method, which is a specific test for the ESR, with modifications, (ii) The reference method for measurement of the ESR should use either whole blood anticoagulated with EDTA and later diluted with sodium citrate or saline (4 : 1) or whole blood anticoagulated with sodium citrate (4 : 1) in Westergren pipettes, (iii) The ESR pipettes can be of glass or plastic (with specific characteristics). It must be colourless; ... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4545284 Fri, 25 Feb 2011 00:00:00 +0100 4545284 http://www.medworm.com/index.php?rid=4545286&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338473%26dopt%3DAbstract Conclusion:  Many of the leukocyte CPD have been identified as useful parameters of sepsis. Hopefully, these parameters can ultimately be incorporated into a decision rule for the screening of sepsis samples and to discriminate fungemia from bacteremia. PMID: 21338473 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4545286 Tue, 22 Feb 2011 00:00:00 +0100 4545286 http://www.medworm.com/index.php?rid=4545285&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338474%26dopt%3DAbstract Conclusion:  The capillary electrophoresis system could clearly demonstrate the presence of abnormal Hbs and provide useful information for presumptive diagnoses in most cases. The Hb analysis results could help in selection of appropriate DNA testing for final diagnoses of these variants. PMID: 21338474 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4545285 Tue, 22 Feb 2011 00:00:00 +0100 4545285 http://www.medworm.com/index.php?rid=4490115&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21310007%26dopt%3DAbstract Conclusion:  MPV has limited sensitivity and specificity to discriminate between megaloblastic and non-megaloblastic pancytopenia. Pancytopenia due to aplastic/hypocellular marrow and acute leukaemia has significantly lower MPV than megaloblastic group while other pancytopenic cases do not show any statistical difference in MPV from megaloblastic pancytopenia. PMID: 21310007 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4490115 Thu, 10 Feb 2011 00:00:00 +0100 4490115 http://www.medworm.com/index.php?rid=4490114&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21310008%26dopt%3DAbstract Conclusion:  Pretransplantation IO might be a major contributor to adverse outcome in HSCT recipients through an impaired pro-oxidative/antioxidative homeostasis. The reversible nature of IO and oxidative stress suggests that early preventive strategies might have a potential to improve transplant outcome. PMID: 21310008 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4490114 Thu, 10 Feb 2011 00:00:00 +0100 4490114 http://www.medworm.com/index.php?rid=4490116&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21284831%26dopt%3DAbstract Conclusion:  In our experience, systematic schistocyte count after HSCT proved to be useful: the occurrence of an increased percentage was a surrogate marker for complications even if unspecific for TM. PMID: 21284831 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4490116 Wed, 02 Feb 2011 00:00:00 +0100 4490116 http://www.medworm.com/index.php?rid=4419879&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21272268%26dopt%3DAbstract Conclusion:  A variety of patterns of the IgH deletion were identified by interphase FISH using IgH break-apart and IgH/CCND1 probes in patients with MM and CLL. The results of this study suggest that the integrated information obtained with IgH break-apart and IgH/CCND1 FISH was needed to interpret FISH results unambiguously. PMID: 21272268 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4419879 Fri, 28 Jan 2011 00:00:00 +0100 4419879 http://www.medworm.com/index.php?rid=4419882&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21266019%26dopt%3DAbstract Conclusion:  A novel StyI restriction enzyme can be used to confirm the commonest type of Hb G-Philadelphia. DNA sequencing identified four other α-chain variants with a similar HPLC and IEF phenotype. PMID: 21266019 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4419882 Wed, 26 Jan 2011 00:00:00 +0100 4419882 http://www.medworm.com/index.php?rid=4419881&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21266020%26dopt%3DAbstract Conclusion:  These findings, when combined with the data on 35INS, support the concept that loss of the C-terminus of BCR-ABL1 is associated with significant resistance to kinase inhibitors; this mechanism appears to be a major source of resistance to kinase inhibitors. PMID: 21266020 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4419881 Wed, 26 Jan 2011 00:00:00 +0100 4419881 http://www.medworm.com/index.php?rid=4419880&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21266021%26dopt%3DAbstract Conclusion:  The Celltac F shows broadly comparable analytical performance to the XE-2100 for the parameters assessed. The Celltac F is recommendable for medium-sized laboratories or as a back-up instrument in larger laboratories. PMID: 21266021 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4419880 Wed, 26 Jan 2011 00:00:00 +0100 4419880 http://www.medworm.com/index.php?rid=4419883&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21251240%26dopt%3DAbstract Conclusion:  Patients with two out of three features, GPC, antithyroid antibodies, gastric atrophy, but without HP organisms; or three features with HP organisms, can be predicted to have PA. PMID: 21251240 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4419883 Thu, 20 Jan 2011 00:00:00 +0100 4419883 http://www.medworm.com/index.php?rid=4356735&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21219591%26dopt%3DAbstract Conclusions:  These results suggest that CIP2A is over-expressed in patients with newly diagnosed/relapsed AML and the expression of CIP2A could have potential use as a clinical marker for AML relapse after treatment. The high expression of CIP2A in HL60 cells may be related to active cell proliferation and arrest of cell differentiation. This study may shed light on the molecular function of CIP2A in myeloid leukemogenesis. PMID: 21219591 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4356735 Tue, 11 Jan 2011 00:00:00 +0100 4356735 http://www.medworm.com/index.php?rid=4259727&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143592%26dopt%3DAbstract Discussion:  Our findings suggest aCGH is an effective technique for evaluating recurring genetic abnormalities in CLL and improves on standard FISH in detecting genetic abnormalities in CLL. PMID: 21143592 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4259727 Thu, 09 Dec 2010 00:00:00 +0100 4259727 http://www.medworm.com/index.php?rid=4228995&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118380%26dopt%3DAbstract Conclusion:  Routine testing for JAK2V617F is not currently recommended for patients with unexplained thromboses, except for those with splanchnic vein thrombosis. In patients with cerebral vein thrombosis, the value of testing for JAK2V617F mutation is yet to be established. PMID: 21118380 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228995 Wed, 01 Dec 2010 00:00:00 +0100 4228995 http://www.medworm.com/index.php?rid=4228994&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118381%26dopt%3DAbstract Conclusion:  Our data strongly support the use of IgG-only assays for the detection of HIT antibodies. PMID: 21118381 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228994 Wed, 01 Dec 2010 00:00:00 +0100 4228994 http://www.medworm.com/index.php?rid=4228988&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118382%26dopt%3DAbstract Conclusion:  Thus, unlike Westergren erythrocyte sedimentation rate, sedimentation data obtained by the proposed method do not require correction for Hct. PMID: 21118382 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228988 Wed, 01 Dec 2010 00:00:00 +0100 4228988 http://www.medworm.com/index.php?rid=4228970&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118383%26dopt%3DAbstract Authors: Liu YN, Li R, Li J, Li DZ PMID: 21118383 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228970 Wed, 01 Dec 2010 00:00:00 +0100 4228970 http://www.medworm.com/index.php?rid=4228964&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118384%26dopt%3DAbstract Authors: Zammit G, Farrugia R, Barbara C, Azzopardi L, Inglott AS, Adami MZ, Grech V PMID: 21118384 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228964 Wed, 01 Dec 2010 00:00:00 +0100 4228964 http://www.medworm.com/index.php?rid=4228963&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118385%26dopt%3DAbstract Conclusion:  In our study group, a bone marrow biopsy was a useful investigation with a high diagnostic yield. PMID: 21118385 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228963 Wed, 01 Dec 2010 00:00:00 +0100 4228963 http://www.medworm.com/index.php?rid=4228962&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118386%26dopt%3DAbstract Authors: Schindhelm RK, Van Der Zwan LP PMID: 21118386 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228962 Wed, 01 Dec 2010 00:00:00 +0100 4228962 http://www.medworm.com/index.php?rid=4228961&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118387%26dopt%3DAbstract Conclusion:  This combination of problems effectively combines to render selective reamplification and redigestion unsuitable for detecting low fractions of the V617F mutation. PMID: 21118387 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228961 Wed, 01 Dec 2010 00:00:00 +0100 4228961 http://www.medworm.com/index.php?rid=4228960&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21118388%26dopt%3DAbstract Conclusion:  Two reagents, namely aPTT-SP and SynthASIL both of which are based on synthetic phospholipids and silica, were identified as promising candidates for incorporation into point-of-care diagnostic device platforms as dried reagents. PMID: 21118388 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4228960 Wed, 01 Dec 2010 00:00:00 +0100 4228960 http://www.medworm.com/index.php?rid=4159420&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054814%26dopt%3DAbstract Authors: Hahm C, Huh J, Hwang YS, Choi HJ, Yang HJ, Chung WS PMID: 21054814 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4159420 Thu, 04 Nov 2010 00:00:00 +0100 4159420 http://www.medworm.com/index.php?rid=4159419&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054815%26dopt%3DAbstract Authors: Kumar R, Agarwal S PMID: 21054815 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4159419 Thu, 04 Nov 2010 00:00:00 +0100 4159419 http://www.medworm.com/index.php?rid=4159425&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054811%26dopt%3DAbstract Conclusion:  Data from ongoing and future studies are awaited to better understand this entity and to highlight the most appropriate treatment strategies. PMID: 21054811 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4159425 Wed, 03 Nov 2010 00:00:00 +0100 4159425 http://www.medworm.com/index.php?rid=4159422&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054812%26dopt%3DAbstract Authors: Lesesve JF, Muller M, Vautrin A, Odinotte A, Etienne Y Laboratory identification of cytoplasmic inclusions in leucocytes as unusual manifestation of cryoglobulinemia has been previously reported (Maitra et al., 2000 American Journal of Clinical Pathology, 113, 107-112; Fohlen-Walter et al., 2002American Journal of Clinical Pathology, 117, 606-614.). We would like to add two observations highlighting the following: (i) the peculiar picture of cryoglobulins in neutrophils and monocytes but sparing other white blood cell (WBC) and (ii) possibility of deposit occurrence with morphological identification in body fluids. PMID: 21054812 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4159422 Wed, 03 Nov 2010 00:00:00 +0100 4159422 http://www.medworm.com/index.php?rid=4159421&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054813%26dopt%3DAbstract Conclusion:  Lesser amounts of EMA-reactive membrane proteins were detected in HPP than HS red cells, thus confirming their lower fluorescence readings in the EMA-binding test. The concomitant reduction in glycophorins A and C, and CD59 in HPP could have caused cellular contraction, resulting in poikilocytosis. PMID: 21054813 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4159421 Wed, 03 Nov 2010 00:00:00 +0100 4159421 http://www.medworm.com/index.php?rid=4111140&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20979595%26dopt%3DAbstract Conclusion:  The data showed a statistically significant difference between glass and plastic tubes in the normal population only for the PT test, with consequent repercussions for patients on OAT. This means that appropriate care and validation should take place whenever there is a change in tube type. PMID: 20979595 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4111140 Tue, 26 Oct 2010 23:00:00 +0100 4111140 http://www.medworm.com/index.php?rid=4082582&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20942870%26dopt%3DAbstract Conclusions:  Thus, the MFI ratio B-CLL/T cell corrected by isotype is a reliable analysis technique to estimate ZAP-70 expression in B-CLL. PMID: 20942870 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4082582 Wed, 06 Oct 2010 23:00:00 +0100 4082582 http://www.medworm.com/index.php?rid=4082583&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20942869%26dopt%3DAbstract Conclusion:  K562r due to duplication of autophosphorylation of wild-type Bcr-Abl induced by imatinib was still partially resistant to dasatinib and nilotinib, but this was overcome by incremental dosing. Rapamycin did not enhance imatinib sensitivity. The blockade of the ubiquitin-proteasome pathway could be effective in overcoming resistance in the K562r imatinib-resistant cell line. PMID: 20942869 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4082583 Sun, 03 Oct 2010 23:00:00 +0100 4082583 http://www.medworm.com/index.php?rid=4031613&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20880301%26dopt%3DAbstract Conclusion:  This review has demonstrated that no significant coagulation factor deficiency would be left undiagnosed if the protocol was followed. This would have considerably reduced the cost and time spent performing these assays. PMID: 20880301 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4031613 Tue, 28 Sep 2010 23:00:00 +0100 4031613 http://www.medworm.com/index.php?rid=4017935&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20868446%26dopt%3DAbstract Conclusion:  Patients with IDA can be differentiated from ACD and ACD/IDA but not ACD from ACD/IDA based on hepcidin-25 alone. The combination of hepcidin-25 with CHr in the hepcidin-25 plot was useful for the differentiation of the states above. PMID: 20868446 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4017935 Sun, 26 Sep 2010 23:00:00 +0100 4017935 http://www.medworm.com/index.php?rid=4017934&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20868447%26dopt%3DAbstract Conclusion:  Since population genetic studies on VNTR loci are not well established in Southeast Asia, the present study is useful to determine reliable markers during the initial screening step of chimerism analysis. By following this algorithm, we are able to reduce time and cost of finding a suitable VNTR marker in our cohort. PMID: 20868447 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4017934 Sun, 26 Sep 2010 23:00:00 +0100 4017934 http://www.medworm.com/index.php?rid=4017936&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20860735%26dopt%3DAbstract Conclusion:  Calculating a ratio between the LA-sensitive PTT-LA and the less sensitive Pathromtin-SL improves the performance of the PTT-LA itself and represents a simple and sensitive aPTT-based integrated strategy for LA screening. PMID: 20860735 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=4017936 Wed, 22 Sep 2010 23:00:00 +0100 4017936 http://www.medworm.com/index.php?rid=3977130&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20840376%26dopt%3DAbstract Conclusion:  Both Synthasil and Actin FS are acceptable reagents to screen for reduced factors VIII, IX and XI, and the number of mildly reduced factors not diagnosed will be limited. PMID: 20840376 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3977130 Mon, 13 Sep 2010 23:00:00 +0100 3977130 http://www.medworm.com/index.php?rid=3977129&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20840377%26dopt%3DAbstract Conclusion:  Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A(2) , and between hemoglobins S and F using the Ultra2 HPLC method. PMID: 20840377 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3977129 Mon, 13 Sep 2010 23:00:00 +0100 3977129 http://www.medworm.com/index.php?rid=3943791&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20813022%26dopt%3DAbstract Conclusion: All evaluated ELISAs are a practical tool in the laboratory diagnosis of APS. The diagnostic performance shows slight differences between the ELISAs from the different manufacturers. PMID: 20813022 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3943791 Mon, 30 Aug 2010 23:00:00 +0100 3943791 http://www.medworm.com/index.php?rid=3904000&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20731731%26dopt%3DAbstract Authors: Son KH, Ahn CB, An H, Choe G, Lee SH, Sun K PMID: 20731731 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3904000 Sat, 21 Aug 2010 23:00:00 +0100 3904000 http://www.medworm.com/index.php?rid=3887248&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20718874%26dopt%3DAbstract Conclusion: In conclusion, our data clearly demonstrate that as long as the World Anti-Doping Agency's guidelines are followed rigorously, all blood results reach the quality level required in the antidoping context. PMID: 20718874 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3887248 Sun, 15 Aug 2010 23:00:00 +0100 3887248 http://www.medworm.com/index.php?rid=3887247&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20718875%26dopt%3DAbstract Conclusion: The DxH 800 provides reliable results and increases laboratory efficiency by reducing working time and costs associated with the optical validation of the results. PMID: 20718875 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3887247 Sun, 15 Aug 2010 23:00:00 +0100 3887247 http://www.medworm.com/index.php?rid=3828210&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20681999%26dopt%3DAbstract Conclusion: Within this paper, we emphasize the importance of microchimerism monitoring as a reliable indicator of incipient AML relapse, especially in patients where no other specific molecular marker is available. PMID: 20681999 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3828210 Sun, 01 Aug 2010 23:00:00 +0100 3828210 http://www.medworm.com/index.php?rid=3828209&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20682000%26dopt%3DAbstract Authors: Lam BS, Adams GB Summary Treatment of malignant blood disorders, such as leukemia, that can provide a better chance of long-term remission involves myeloablation followed by transplantation of matched donor hematopoietic stem cells (HSCs). For successful engraftment and re-establishment of hematopoiesis to occur in the recipient, the transplanted HSCs must first migrate from the blood circulation to the bone marrow (BM), a process known as homing, then localize and anchor in suitable microenvironments within the BM, a process known as lodgment. After lodgment, the specific fate of the transplanted HSCs is determined through complex, bidirectional interactions with various stromal cell components in the niche. Ultimately, these interactions dictate the clinical outcome of the t... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3828209 Sun, 01 Aug 2010 23:00:00 +0100 3828209 http://www.medworm.com/index.php?rid=3828208&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20682001%26dopt%3DAbstract Conclusion: Based on some key features for an activated CD95 system, this death receptor has been considered to induce PS exposure. However, we give evidence, that CD95 is not functional in red blood cells and that activation of this death receptor does not lead to the exposure of phosphatidylserine. PMID: 20682001 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3828208 Sun, 01 Aug 2010 23:00:00 +0100 3828208 http://www.medworm.com/index.php?rid=3828212&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20678137%26dopt%3DAbstract Conclusion: HbA2 mutations either structurally stable and visible or undetectable because of a thalassemia effect or instability are clinically asymptomatic but may compromise the diagnosis of beta-thalassemia minor. Stable mutations result in two HbA2 fractions of about half of the expected value. Expression defects are undetectable as a protein fraction but reduce the amount of HbA2 by half. PMID: 20678137 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3828212 Wed, 28 Jul 2010 23:00:00 +0100 3828212 http://www.medworm.com/index.php?rid=3813987&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20670338%26dopt%3DAbstract Conclusion: Including the GI index in the routine parameters provided by the Sysmex analyzer could be of major help for nonspecialized routine laboratories in detecting MDS. PMID: 20670338 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3813987 Wed, 28 Jul 2010 23:00:00 +0100 3813987 http://www.medworm.com/index.php?rid=3813989&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20666852%26dopt%3DAbstract Conclusion: CD66c expression is correlated, but not specifically, with BCR/ABL1 rearrangement. It would seem better to interpret the absence of CD66c expression with a lack of BCR/ABL1 rearrangement. This myeloid antigen could be interesting in the detection of minimal residual disease. PMID: 20666852 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3813989 Tue, 27 Jul 2010 23:00:00 +0100 3813989 http://www.medworm.com/index.php?rid=3813988&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20670271%26dopt%3DAbstract Conclusion: The most appropriate classification of myeloid neoplasms presenting with hybrid myelodysplastic/myeloproliferative features requires a comprehensive clinical and laboratory assessment with careful integration of the morphological, immunophenotypic, genetic, and clinical characteristics. PMID: 20670271 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3813988 Tue, 27 Jul 2010 23:00:00 +0100 3813988 http://www.medworm.com/index.php?rid=3787427&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20649908%26dopt%3DAbstract Conclusion: Patient B is the second report of the CD54-58(-13 bp) deletion. However, our report differs from the previous report in two ways: we performed a family study based on multiple samples; and the carriers and patient showed an elevated level of HbF, which was not observed in the previous case. PMID: 20649908 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3787427 Thu, 22 Jul 2010 23:00:00 +0100 3787427 http://www.medworm.com/index.php?rid=3787426&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20649909%26dopt%3DAbstract Conclusion: Atypical derivative curves seen in ellagic acid APTTs are of limited diagnostic use because of the frequency with which they occur. This may be related to the need to optimize the data reduction utilized on the ACL TOP for these reagents. With silica activator APTTs, the presence of atypical derivative curves proved to be a very simple tool when troubleshooting unexpected abnormal APTT results, commonly predicting a factor deficiency or LA that would warrant further investigation. The cause of these aberrant derivative curves is probably related to abnormal thrombin generation in the APTT test and warrants further study. PMID: 20649909 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3787426 Thu, 22 Jul 2010 23:00:00 +0100 3787426 http://www.medworm.com/index.php?rid=3762577&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20633055%26dopt%3DAbstract Authors: Mamtani M, Chatterjee N, Mishra A, Soni R, Jawahirani A, Das K, Rughwani V, Shrivastava M, Kulkarni H PMID: 20633055 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3762577 Mon, 12 Jul 2010 23:00:00 +0100 3762577 http://www.medworm.com/index.php?rid=3762578&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20626469%26dopt%3DAbstract Authors: Yin CC, Medeiros LJ, Bueso-Ramos CE Summary The fourth edition of the World Health Organization (WHO) classification of myeloid neoplasms refined the criteria for some previously described myeloid neoplasms and recognized several new entities based on recent elucidation of molecular pathogenesis, identification of new diagnostic and prognostic markers, and progress in clinical management. Protein tyrosine kinase abnormalities, including translocations or mutations involving ABL1, JAK2, MPL, KIT, PDGFRA, PDGFRB, and FGFR1, have been used as the basis for classifying myeloproliferative neoplasms (MPN). Two new entities - refractory cytopenia with unilineage dysplasia and refractory cytopenia of childhood have been added to the group of myelodysplastic syndromes (MDS), and 'refra... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3762578 Tue, 06 Jul 2010 23:00:00 +0100 3762578 http://www.medworm.com/index.php?rid=3684570&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20561093%26dopt%3DAbstract This study was successful in reducing the interinstitutional variability of CD34+ enumeration. It was shown that the implementation of a standardized protocol does not guarantee accurate measurement. Our research design represents a useful tool, which allows verification of the proper use of a standardized method, the training of operators and feedback in response to the survey results. PMID: 20561093 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3684570 Tue, 15 Jun 2010 23:00:00 +0100 3684570 http://www.medworm.com/index.php?rid=3604078&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20497486%26dopt%3DAbstract Authors: Nsiah K, Dzogbefia VP, Ansong D, Boateng H, Ocloo D, Osei-Frempong E, Kena Frempong N, Osei Akoto A Summary Hemolytic anemia is common in sickle cell disease (SCD), but the course and extent differ, depending on genetic, epigenetic, and environmental factors. In the malaria-endemic tropical environment, some vulnerable subjects would be infected and the impact of infection would vary. Therefore, this study was to find malaria incidence and the associated changes in some laboratory indices in 330 SCD subjects. Following blood smear preparation for falciparum detection, hematological and biochemical indices were measured for a comparison of parasitemic and age-matched, genotype-matched, and sex-matched nonparasitemics. For sixty-nine parasitemics, constituting about 21% of all s... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604078 Sat, 22 May 2010 23:00:00 +0100 3604078 http://www.medworm.com/index.php?rid=3604080&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20492000%26dopt%3DAbstract Authors: Urrechaga E, Borque L, Escanero JF Summary Cell counter-based formulae have been used in the differential diagnosis of microcytic anemia. The measurement of new red cell parameters is now available on the Sysmex XE 5000 analyzer. Derived from the percentages of microcytic and hypochromic red cells, the authors describe the new formula %microcytic-%hypochromic, M-H index. The aim of this study was to assess the discriminant value of this new index in the differential diagnosis of microcytic anemia and thalassemia screening compared to the published indices. Receiver operating characteristic curves, sensitivity, specificity and Youden index were calculated for a set of 170 iron-deficiency anemia patients and 200 beta thalassemia carriers. % microcytic-% hypochromic index showed ... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604080 Mon, 17 May 2010 23:00:00 +0100 3604080 http://www.medworm.com/index.php?rid=3604079&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20492001%26dopt%3DAbstract Authors: Li XY, Li JH, Jiang Y, Peng BS, Li J, Zhu SL, Su ZW, Chang JP PMID: 20492001 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604079 Mon, 17 May 2010 23:00:00 +0100 3604079 http://www.medworm.com/index.php?rid=3604085&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20491995%26dopt%3DAbstract Authors: Dzietczenia J, Wróbel T, Jaźwiec B, Mazur G, Butrym A, Poręba R, Kuliczkowski K Summary Bone morphogenetic proteins (BMPs) are multifunctional cytokines which belong to transforming growth factor beta (TGF beta) superfamily. They regulate proliferation, differentiation, and apoptosis in a variety of cells including hematopoietic cells. BMPs act because of binding to two types of serine/threonine kinase receptors: BMP type I receptors (IA and IB) and BMP type II receptor. Deregulation of BMPs signaling pathways has been reported in some of human cancers, but the role of BMPs in hematopoietic malignancies remains unknown. The aim of our study was to examine the percentage of expression of BMPs receptors on lymphocytes of patients with B-cell chronic lymphocytic leukemia... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604085 Sun, 09 May 2010 23:00:00 +0100 3604085 http://www.medworm.com/index.php?rid=3604084&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20491996%26dopt%3DAbstract Authors: Hedley BD, Keeney M, Chin-Yee I, Brown W Summary The Beckman Coulter UniCel((R)) DxH 800 is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC, white blood cell (WBC) differential, nucleated red blood cell (NRBC), and reticulocyte analysis. Evaluation of this instrument was performed in our 800-bed tertiary care hospital and specifically centered upon the correlation of WBC, NRBC, and platelet (PLT) enumeration when compared to a predicate analyzer, the Coulter((R)) LH 780, and flow cytometry (FCM) reference methods. Of particular interest were those samples with morphologically confirmed interference and extreme leukocytosis (evaluated with respect to red blood cell parameter correction). The sample set (... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604084 Sun, 09 May 2010 23:00:00 +0100 3604084 http://www.medworm.com/index.php?rid=3604083&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20491997%26dopt%3DAbstract Authors: Bartley PA, Ross DM, Latham S, Martin-Harris MH, Budgen B, Wilczek V, Branford S, Hughes TP, Morley AA Summary Increasing numbers of patients with chronic myeloid leukaemia (CML) treated with tyrosine kinase inhibitors achieve undetectable levels of BCR-ABL mRNA using sensitive quantitative real-time reverse transcriptase PCR (RT-qPCR) methods and a method to measure minimal residual disease (MRD) in patients with low levels could be of value. Following isolation and sequencing of the patient-specific BCR-ABL breakpoint, a DNA-based nested qPCR assay was established, and MRD was measured by this method and one-round RT-qPCR in 38 samples from 24 patients with CML. Mixing experiments using patient DNA in normal DNA indicated that DNA qPCR could detect BCR-ABL sequences at a lim... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604083 Sun, 09 May 2010 23:00:00 +0100 3604083 http://www.medworm.com/index.php?rid=3604082&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20491998%26dopt%3DAbstract Authors: Urrechaga E, Borque L, Escanero JF Summary The reticulocyte hemoglobin equivalent (Ret He) represents an indirect measure of the functional iron available for erythropoiesis over the previous 2-3 days. Only the analyzers of a single manufacturer, Sysmex (Sysmex Corporation, Kobe, Japan), include Ret He. Red blood cell size factor (RSf) is a new parameter provided by Beckman Coulter, which joins together the volume of the erythrocytes and the volume of reticulocytes. The aims of the study were to investigate the clinical usefulness of RSf in the study of erythropoiesis status and to assess its concordance with Ret He values. Samples from 417 patients were run on both LH 780 (Beckman Coulter) and Sysmex XE 5000 analyzers. Independent samples t-test, Pearson correlation, receiver... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604082 Sun, 09 May 2010 23:00:00 +0100 3604082 http://www.medworm.com/index.php?rid=3604081&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20491999%26dopt%3DAbstract Authors: McLornan DP, Burthem J, Duncombe A, Hatton C, Hutchinson CV, Marsden K, Macartney CM, Smith-Straney T, Uprichard J, Wallis J, Webb S, Wilkins BS, McMullin MF Summary Seven cases were discussed by an expert panel at the 2009 Annual Scientific Meeting of the British Society of Haematology. These cases are presented in a similar format to that adopted for the meeting. There was an initial discussion of the presenting morphology, generation of differential diagnoses and then, following display of further presenting and diagnostic information, each case was concluded with provision of a final diagnosis. PMID: 20491999 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3604081 Sun, 09 May 2010 23:00:00 +0100 3604081 http://www.medworm.com/index.php?rid=3557591&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20456519%26dopt%3DAbstract Authors: Bao X, Hou L, Sun A, Chen M, Chen Z, He J Summary The KIR2DS4 variants differ in exon 5 and play a role in hematopoietic stem cells transplantation (HSCT). A sequence-based testing (SBT) and TOPO TA cloning system identifying and distinguishing alleles of the KIR2DS4 gene was established and applied to a total of 150 Chinese-Han individuals: 75 patients received T-cell-depleted HSCT and their unrelated donors. The majority (139) of the 150 samples (92.7%) were positive for KIR2DS4. Four of the nine known KIR2DS4 alleles, KIR2DS4 *00101, *003,*004, and *007, were identified. In the haplotype A/A group, a higher risk of acute graft-versus-host disease (aGVHD) was seen when the donor carried two full-length KIR2DS4 alleles (RR 9.0 [95% CI 1.2-66.9], P = 0.010). Our findings sugge... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3557591 Thu, 22 Apr 2010 23:00:00 +0100 3557591 http://www.medworm.com/index.php?rid=3557584&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20456520%26dopt%3DAbstract Authors: Yamaoka G, Kubota Y, Nomura T, Inage T, Arai T, Kitanaka A, Saigo K, Iseki K, Baba N, Taminato T Summary Prediction of the timing of platelet recovery after chemotherapy and hematopoietic stem cell transplantation (HSCT) allows for optimal platelet transfusion. We assessed the clinical utility of the percentage value of the immature platelet fraction (IPF%) monitored using an XE-2100 automated hematology analyzer to predict the timing of platelet recovery after chemotherapy and HSCT. The IPF% was serially monitored in 31 patients with cancer who received 66 courses of chemotherapy and HSCT. In patients with cancer undergoing chemotherapy and HSCT, a transient increase in IPF% was observed 1-11 days prior to platelet recovery (>30 x 10(9)/l). In patients undergoing chemother... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3557584 Thu, 22 Apr 2010 23:00:00 +0100 3557584 http://www.medworm.com/index.php?rid=3494745&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20402824%26dopt%3DAbstract Authors: Cembrowski GS, Smith B, Tung D Summary Diverse approaches have been used to assure the analytical quality of automated hematology; as such, there is great variation in their error detection capabilities. We summarize the intralaboratory performance of a cohort of Sysmex XE-2100's running e-Check hematology quality control (QC). The imprecisions of a median performing (50th percentile imprecision) and more imprecise [15th percentile (15P) imprecision] Sysmex XE-2100 are compared with measures of total allowable error (regulatory and physiologically based) to obtain multiples of the usual imprecision that must be detected to prevent the hematology analyzer from producing medically unacceptable results. The resultant large multiples of the usual imprecision (s) demonstrate the ne... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3494745 Thu, 15 Apr 2010 23:00:00 +0100 3494745 http://www.medworm.com/index.php?rid=3494746&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20402823%26dopt%3DAbstract Authors: Leers MP, Goertz H, Feller A, Hoffmann JJ Summary Two mid-range haematology analysers (Abbott CELL-DYN Ruby and Sysmex XT-2000i) were evaluated to determine their analytical performance and workflow efficiency in the haematology laboratory. In total 418 samples were processed for determining equivalence of complete blood count (CBC) measurements, and 100 for reticulocyte comparison. Blood smears served for assessing the agreement of the differential counts. Inter-instrument agreement for most parameters was good although small numbers of discrepancies were observed. Systematic biases were found for mean cell volume, reticulocytes, platelets and mean platelet volume. CELL-DYN Ruby WBC differentials were obtained with all samples while the XT-2000i suppressed differentials parti... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3494746 Mon, 05 Apr 2010 23:00:00 +0100 3494746 http://www.medworm.com/index.php?rid=3404314&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20331762%26dopt%3DAbstract Authors: Le Bars H, Boulland ML, Bareau B, Grosbois B, Corolleur M, Lamy T, Fardel O PMID: 20331762 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3404314 Tue, 23 Mar 2010 00:00:00 +0100 3404314 http://www.medworm.com/index.php?rid=3404313&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20331763%26dopt%3DAbstract Authors: Zhang SJ, Qiu HX, Li JY, Shi JY, Xu W Summary Recent studies have shown that JAK2 V617F, MPL W515L/K and JAK2 exon 12 mutations underlie the major molecular pathogenesis of myeloproliferative disorders (MPN). Allele-Specific Polymerase Chain Reaction (AS-PCR), direct sequencing and MassARRAY assay were used to ascertain the real prevalence of these mutations and the influence of genetic susceptibility in Chinese MPN patients. The positive rate of JAK2 V617F in polycythaemia vera (PV), essential thrombocythaemia (ET) and primary myelofibrosis (PMF) was 82.0%, 36.6% and 51.1% respectively. One ET patient and two PMF patients harboured the MPL W515L mutation and three PV patients harboured JAK2 exon 12 mutations. All of these patients were confirmed as JAK2 V617F negative. Clinic... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3404313 Tue, 23 Mar 2010 00:00:00 +0100 3404313 http://www.medworm.com/index.php?rid=3383581&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20236183%26dopt%3DAbstract Authors: Paris A, Nhan T, Cornet E, Perol JP, Malet M, Troussard X Summary We evaluated the performance of the automated body fluid mode of the Sysmex XE-5000 series automated haematology analyzer and compared the performance of the automated method for obtaining white blood cell (WBC), red blood cell (RBC) counts and WBC differential counts with microscopic method. One hundred and seventy-four samples were analysed: 81 ascitic fluid, 32 cerebrospinal fluid (CSF), 26 pleural fluid (PF), 18 synovial fluid (SF), 13 peritoneal fluid (PeF) and 4 other types. The agreement between the automated method and the manual reference showed high correlation, with Pearson correlation coefficients greater than 0.9 for all types of body fluids. We also demonstrate that the automated body fluid analysi... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3383581 Wed, 03 Mar 2010 00:00:00 +0100 3383581 http://www.medworm.com/index.php?rid=3383580&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20236184%26dopt%3DAbstract The objective is to establish race-specific reference intervals for WBC and ANC using US National Health and Nutrition Examination Survey (NHANES) of 2000-2003. A total of 14 184 civilian noninstitutionalized US citizens participated in NHANES 2000-2003 had complete blood count, red cell distribution width, platelet count and automated WBC differential determined on a Coulter MAXM. The exclusion criteria were used: ferritin <12 ng/ml, pregnancy, body mass index >30, diastolic blood pressure >100 mm Hg, creatinine >2.5 mg/dl, glucose >126 mg/dl. Data were separated into six sex/race categories: female non-Hispanic white, non-Hispanic black (NHBF)], Mexican American; male non-Hispanic white, non-Hispanic black (NHBM), Mexican American and two age groupings (12-18 and >18 ye... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3383580 Wed, 03 Mar 2010 00:00:00 +0100 3383580 http://www.medworm.com/index.php?rid=3343070&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201994%26dopt%3DAbstract Conclusion] These results indicate that MPXI is correlated with some specific infectious states, i.e. MPXI is low in bacterial sepsis and high in nontuberculous nonseptic bacterial infections. MPXI appears to be an independent and useful biomarker for the diagnosis and follow-up of infectious diseases, especially when the MPXI values are obtained at regular intervals during the disease courses of the patients. PMID: 20201994 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343070 Thu, 25 Feb 2010 00:00:00 +0100 3343070 http://www.medworm.com/index.php?rid=3343069&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201995%26dopt%3DAbstract Authors: Islam MS, Anoop P PMID: 20201995 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343069 Thu, 25 Feb 2010 00:00:00 +0100 3343069 http://www.medworm.com/index.php?rid=3343068&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201996%26dopt%3DAbstract Authors: Gutiérrez A, Martínez-Serra J, Barceló B, Sampol A, Viñas L, González G, Bea MD, Amat JC, Martín J, Ramos R, Bautista A, Forteza-Rey J, Rodríguez J, Besalduch J Summary CA125, a tumor marker normally used to follow the clinical course of ovarian cancer, also may have a role in lymphoma. All available series were analyzed using the standard reference value 35 U/ml, but age and sex may influence serum CA125 (sCA125) levels. We aim to study the prognostic value of serum CA125 (sCA125) levels in diffuse large B-cell lymphoma (DLBCL), considering the influence of age and sex on sCA125 levels. We investigated the relationship between sCA125 and clinical outcome after treatment in 42 patients with DLBCL, comparing both the standard (35 U/ml) and a n... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343068 Thu, 25 Feb 2010 00:00:00 +0100 3343068 http://www.medworm.com/index.php?rid=3343067&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201997%26dopt%3DAbstract Authors: Inati A, Musallam KM, Cappellini MD, Duca L, Taher AT Summary The value of nontransferrin-bound iron (NTBI) as an index of iron overload in patients with thalassemia has been evaluated; however, data in patients with sickle cell disease (SCD) is limited. NTBI levels were evaluated in a cross-sectional study of 43 transfused patients with SCD. Patient charts were reviewed for demographics, status of the spleen, and total number of lifetime transfusions. All patients were chelation naïve and none of the patients had evidence of hepatitis B or C infection. Blood samples were taken for assessment of NTBI and serum ferritin (SF); liver iron concentration (LIC) was determined by R2 magnetic resonance imaging. NTBI levels were generally low with a median of -0.01 mum (range -2.5... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343067 Thu, 25 Feb 2010 00:00:00 +0100 3343067 http://www.medworm.com/index.php?rid=3343066&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201998%26dopt%3DAbstract Conclusion: We have characterised the CD138(-) PCs as more immature and with a significantly higher proliferative potential. The current trend to ignore this more immature and proliferative subpopulation of malignant PCs may have serious implications when determining gene expression, classifications and drug sensitivity of the malignancy. PMID: 20201998 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343066 Thu, 25 Feb 2010 00:00:00 +0100 3343066 http://www.medworm.com/index.php?rid=3343065&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20201999%26dopt%3DAbstract Authors: Pawlus J, Hołub M, Kożuch M, Dąbrowska M, Dobrzycki S Summary Early prediction of coronary artery disease complications is vital for the prevention and effective treatment of patients with coronary cardiac disease. It has been reported that inflammatory markers play a key role in the progression of cardiovascular diseases. Platelet count and platelet morphological parameters were analyzed on a fully-automated hematological analyzer ADVIA 2120 (Siemens). Serum myeloperoxidase (MPO) level was determined with an enzyme immunoassay (BioCheck). The measuring range of this assay is between 0 and 40 ng/ml. We demonstrate that serum MPO concentration and platelet activation increase systematically with the advancement of coronary artery disease. Moreover, MPO level i... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3343065 Thu, 25 Feb 2010 00:00:00 +0100 3343065 http://www.medworm.com/index.php?rid=3313299&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20175793%26dopt%3DAbstract In this study, we assessed the diagnostic value of the so-called Thomas'-plot [soluble transferrin receptor (sTfR)/log ferritin (sTfr/log Ferr) and the reticulocyte hemoglobin equivalent (Ret-HE)] in the anemia work up of patients referred by general practitioners. During July 2008-March 2009, 337 consecutive patients were included because of lowered Hb values. The laboratory results of the first 133 consecutive patients were used to determine the cut-off values for the diagnostic plot. The laboratory results of these patients were assessed and interpreted independently by two investigators, blinded from sTfR/log Ferr and Ret-HE values. The following 204 patients were used to test the plot in practice. In 32% of the first 133 patients, no indication of the cause of anemia could be found. H... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3313299 Wed, 17 Feb 2010 00:00:00 +0100 3313299 http://www.medworm.com/index.php?rid=3270360&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20148986%26dopt%3DAbstract Authors: Attia FM, Tawfik GA, Kalil KA, Mossalam MF Summary One of the clinical consequences of aberrant cytokines production in patients with end stage renal disease (ESRD) may be impaired erythropoiesis. To determine the interleukin (IL)-10 levels in ESRD patients on regular hemodialysis (HD) with good and poor response to recombinant human erythropoietin (Epo). Two groups of ESRD-HD patients were evaluated; 48 high epo HD patients and 32 low epo HD patients were evaluated for some laboratory tests and Interleukin-10 by ELISA. The production of IL-10 is decreased in HD with low epo group than high epo group 32.4 +/- 7.9 vs. 45 +/- 6.9 pg/ml (P < 0.001). IL-10 level is well correlated with CRP, ESR, Ferritin, Epo dose, and EPO/Hb ratio in ESRD-HD patients. These findings suggest th... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3270360 Tue, 09 Feb 2010 00:00:00 +0100 3270360 http://www.medworm.com/index.php?rid=3270359&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20148987%26dopt%3DAbstract Conclusion: The Biosite Triage D-dimer assay performed on either citrate or EDTA samples is comparable with the Stago Liatest laboratory D-dimer assay when used in conjunction with clinical pretest probability scoring and CUS for the exclusion of DVT. PMID: 20148987 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3270359 Tue, 09 Feb 2010 00:00:00 +0100 3270359 http://www.medworm.com/index.php?rid=3270358&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20148988%26dopt%3DAbstract Authors: Ahlgrim C, Pottgiesser T, Robinson N, Sottas PE, Ruecker G, Schumacher YO Summary Haemoglobin (Hb) and haematocrit (Hct) are measured as indirect markers of doping in athletes. We studied the effect of posture on these parameters in a typical antidoping setting. Venous blood samples were obtained from nine endurance athletes (six males, three females) and nine control subjects (six males, three females) immediately and after 5, 10, 15, 20 and 30 min after having adopted a seated position from normal daily activity. Hb (CV 0.72%) and Hct (CV 0.87%) were determined using an automated cell counter, plasma volume changes were calculated. Differences between the time points, gender and groups were calculated using a mixed-model procedure. Significant changes were observed in the fi... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3270358 Tue, 09 Feb 2010 00:00:00 +0100 3270358 http://www.medworm.com/index.php?rid=3265728&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20136848%26dopt%3DAbstract Authors: Angalena R, Prabitha KN, Chaudhary AK, Bashyam MD, Jain S, Dalal AB PMID: 20136848 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3265728 Tue, 02 Feb 2010 00:00:00 +0100 3265728 http://www.medworm.com/index.php?rid=3265727&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20136849%26dopt%3DAbstract Authors: Broséus J, Visomblain B, Guy J, Maynadié M, Girodon F Summary Hereditary spherocytosis (HS) is a common red blood cell disorder. It has been shown that the mean sphered corpuscular volume (MSCV), an artificial volume, is always lower than the MCV in HS and also in some autoimmune haemolytic anaemia (AIHA). Our purpose was to assess the reliability of MSCV in routine practise, and its relevance in screening for HS. Comparison of MSCV and MCV was undertaken in a prospective study of 366 patients with anaemia. In addition, included were patients previously diagnosed to have HS (n = 33) or AIHA (n = 16). When MSCV was lower than MCV, a flow cytometric (FC) test for HS was performed. Delta (MCV-MSCV) values >9.6 fl were obtained for all HS patients. A wider spread of d... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3265727 Tue, 02 Feb 2010 00:00:00 +0100 3265727 http://www.medworm.com/index.php?rid=3247420&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20132350%26dopt%3DAbstract Authors: Billard M, Lainey E, Armoogum P, Alberti C, Fenneteau O, DA Costa L Summary The DM is an automated microscope, which performs WBC differential counts and monitors red cell morphology. The user either validates the cell recognition if the DM has correctly identified the WBCs or reclassifies the WBCs in the good category in case of a DM mis-assignment. Morphological anomalies of leukocytes, red blood cells or platelets are analyzed and registered. We studied 521 newborns and infants sorted by age and pathology. The results correlated well with those using conventional microscopy except for samples containing blasts, in which the percentage of malignant cells was underestimated. Newborns had the lowest rates of overall accuracy and postclassification agreement. For red cell analy... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3247420 Tue, 02 Feb 2010 00:00:00 +0100 3247420 http://www.medworm.com/index.php?rid=3198260&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20088999%26dopt%3DAbstract This study aimed to develop a flow cytometric (FCM) method for the immunophenotipic detection and enumeration of these cells in a healthy population. Peripheral blood samples from 32 subjects were analysed. Multiparameter FCM analysis was used to quantify resting and activated CEC and CEP. The mean values of the percentage and of the absolute number were: 0.005 +/- 0.004% and 306 +/- 243 cells/ml for CEC; 0.002 +/- 0.001% and 130 +/- 110 cells/ml for rCEC; 0.003 +/- 0.002% and 176 +/- 150 cells/ml for aCEC; 0.0001 +/- 0.00005% and 6 +/- 2 for CEP. We confirmed that FCM is an accurate and sensitive method for the quantitative analysis of CEC and CEP. The determination of normal ranges of CEC and CEP is helpful in defining their role as surrogate biomarkers of antiangiogenic treatment effica... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3198260 Sat, 23 Jan 2010 02:16:11 +0100 3198260 http://www.medworm.com/index.php?rid=3198259&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20089000%26dopt%3DAbstract In this study, M-FISH was used in 16 patients with de novo MDS and 22 with AML with CCA detected by R-banding CC, and revealed 206 aberrations involved all 24 chromosomes, including 73 numerical chromosomal abnormalities and 133 structural abnormalities. The chromosomes most often involved were, by decreasing incidence, 5, 17, 8, 11, 7 and 21 in 57.9%, 55.3%, 44.7%, 36.8%, 34.2% and 34.2% of the cases, respectively. There were 98 unbalanced translocations, which were the most frequently observed aberrations in our study. Derivative chromosome 5 and 8 were implicated most often. The other derivatives were der(11), der(12), der(7), der(14), der(15) and der(17). Fourteen balanced translocations were detected in our series, and the most frequent reciprocal translocations was t(8;21). Fifty-fiv... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3198259 Sat, 23 Jan 2010 02:16:08 +0100 3198259 http://www.medworm.com/index.php?rid=3198258&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20089001%26dopt%3DAbstract We report the clinicopathologic features of eight patients with aberrant CD4(+), cytotoxic T-cell lymphocytoses. Median follow-up was 29 months (range 8-100), during which all were alive without requirement for therapy. Four of eight patients had an additional malignancy; none had a history of rheumatoid arthritis, lymphadenopathy or hepatosplenomegaly. Morphologic expansions of granulated lymphocytes were evident in 6/8. All had immunophenotypically aberrant populations of CD4(+) T cells with uniform, moderate or bright CD56. Seven of eight expressed CD57, and four were CD8(partial dim +). Abnormal levels of expression of two or more T-cell antigens were seen in all cases. All tested cases were Tgamma PCR positive. Our results support that CD4(+) T-LGL lymphocytosis is a clonal disorder w... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3198258 Sat, 23 Jan 2010 02:16:05 +0100 3198258 http://www.medworm.com/index.php?rid=3174754&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20067547%26dopt%3DAbstract This study was conducted to analyze the role of mean platelet volume (MPV) as a guide or an indicator for bone marrow disease in thrombocytopenic patients. All the patients with thrombocytopenia for various causes followed by bone marrow examination were divided into two groups, one group with and another without bone marrow disease, depending on pathophysiology. The MPV was statistically analyzed in both the groups to assess its role as guide for bone marrow disease in these patients. Mean MPV (average score of all individual mean values in patients) in the group with bone marrow disease was 7.3 fl, while in the group without bone marrow disease, it was 8.62 fl. Although the difference in MPV in the two groups of with (including megaloblastic anemia) and without bone marrow involvement wa... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3174754 Mon, 11 Jan 2010 00:00:00 +0100 3174754 http://www.medworm.com/index.php?rid=3136417&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20041967%26dopt%3DAbstract Authors: Codispoti KE, Depalma L Summary Unexplained anemia in the elderly could represent myelodysplastic syndrome (MDS). We assessed the utility of using a fluorescence in situ hybridization (FISH) panel for common chromosomal abnormalities seen in MDS. A total of 101 elderly outpatients with anemia of unknown etiology were evaluated. Complete blood count, bone marrow biopsy, conventional cytogenetic analysis (CC), and FISH panel were reviewed. A total of 21 (21%) of the 101 patients had MDS. A combination of CC and FISH identified chromosomal abnormalities in 17 (81%) of the patients with MDS. The remaining 4 (19%) were diagnosed with MDS based solely on morphologic criteria. Except in two cases, FISH did not reveal abnormalities not already detected by CC. Furthermore, MDS patients... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3136417 Wed, 23 Dec 2009 00:00:00 +0100 3136417 http://www.medworm.com/index.php?rid=3136416&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20041968%26dopt%3DAbstract Authors: Xu M, Robbe VA, Jack RM, Rutledge JC Summary Current laboratory standards from Clinical Laboratory Standards Institute (CLSI) and manufacturer's (Becton Dickinson) data indicate that under-filling K(2)EDTA blood collection tubes can result in erroneous hematology values. To accommodate under-filled tubes and reduce collection volumes while optimizing our automation, we explored the acceptable limit of under-filled tubes for hematology values. We collected 8.0 ml of blood from 30 normal adult volunteers. Each donation was aliquoted in the following volumes: 4.0, 2.0, 1.0, 0.5 ml x 2. These samples were analyzed within 1 h of blood collection on Sysmex XE-2100 (Sysmex America Inc., Mundelein, IL, USA) for complete blood count, reticulocyte, and white blood cell differentials. Re... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3136416 Wed, 23 Dec 2009 00:00:00 +0100 3136416 http://www.medworm.com/index.php?rid=3004793&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19909382%26dopt%3DAbstract This study was conducted to establish age-related reference ranges for two heparin-binding proteins--vitronectin and platelet factor 4 (PF4)--and to determine if the quantitative values of these proteins may contribute to the reported age-dependent effect of unfractionated heparin (UFH). Plasma samples were obtained from healthy children aged between 1 month and 16 years and from healthy adult volunteers. Two commercial kits were used to measure plasma vitronectin and PF4 levels. Results were reported as mean and boundaries including 95% of the population. Plasma vitronectin levels for children aged 1-5 years were significantly higher compared with adults. Plasma PF4 levels for infants <1 year of age were significantly lower compared with adults. The differences between reference values... International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3004793 Thu, 19 Nov 2009 04:32:08 +0100 3004793 http://www.medworm.com/index.php?rid=3004792&cid=s_36719_19_f&fid=36719&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19909383%26dopt%3DAbstract Authors: Ntaios G, Papadopoulos A, Chatzinikolaou A, Girtovitis F, Kaiafa G, Savopoulos C, Hatzitolios A PMID: 19909383 [PubMed - in process] (Source: International Journal of Laboratory Hematology) International Journal of Laboratory Hematology journals http://www.medworm.com/rss/comments.php?id=3004792 Thu, 19 Nov 2009 04:32:05 +0100 3004792