MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Molecular Medicine' source. Sun, 21 Mar 2010 17:04:07 +0100 http://www.medworm.com/index.php?rid=3328204&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198295%26dopt%3DAbstract Authors: Holle GE This is an overview of the pathophysiological abnormalities of gastroduodenal (GD) ulcers [duodenal ulcer (DU), gastric ulcer (GU) and Dragstedt ulcers (combined DU and GD)], as well as the effects of the different treatments (surgical, medicinal and physiological) described since the introduction of stomach resections. The intention is to demonstrate whether the peptic ulcer diseases are a homogeneous entity with a characteristic pathophysiology or whether they represent the final expression of many heterogeneous causes including impairment of upper gastrointestinal motility. The review also asks whether DU and GU have a common or different pathogenesis and whether ulcers in the stomach might be predominantly due to impaired mucosal resistance and the DU to gastric h... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328204 Thu, 04 Mar 2010 13:23:06 +0100 3328204 http://www.medworm.com/index.php?rid=3328203&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198296%26dopt%3DAbstract Authors: Bao XH, Naomoto Y, Hao HF, Watanabe N, Sakurama K, Noma K, Motoki T, Tomono Y, Fukazawa T, Shirakawa Y, Yamatsuji T, Matsuoka J, Takaoka M Autophagy is a cellular lysosomal degradation pathway involved in proteins and organelles recycling for promoting cell survival, development and homeostasis. It is a multistep process and genetic studies have identified many proteins that participate in autophagosome formation and fusion with lysosomes, and various signaling factors that associate with the regulation of autophagy. In general, autophagy acts as a cell protector and its dysfunction is correlated with diverse pathologies, such as neurodegeneration, liver, heart and muscle diseases, cancer, inflammation and ageing. However, its role in cell death increases the complexity of the... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328203 Thu, 04 Mar 2010 13:23:03 +0100 3328203 http://www.medworm.com/index.php?rid=3328202&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198297%26dopt%3DAbstract This study was aimed at identifying OXPAT and TIP47 transcripts in rat hepatocytes, and investigating how their expression is modulated by excess lipids, using fat-enriched hepatocytes to mimic different degrees of steatosis. Primary rat hepatocytes were exposed to fatty acids (FFAs) for 12, 24 and 36 h. Lipid accumulation was estimated by spectrophotometric quantification of triacylglycerol. Expression of PAT proteins as well as of PPARgamma was evaluated by real-time RT-PCR. Hepatocytes exposed to FFAs showed progressive lipid accumulation. The increase in lipid content was associated with the induction of PAT protein expression. At 12 h, OXPAT and TIP47 mRNA expression was up-regulated. At longer times, the level of OXPAT transcripts remained high, whereas that of TIP47 slowly declined.... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328202 Thu, 04 Mar 2010 13:23:01 +0100 3328202 http://www.medworm.com/index.php?rid=3328201&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198298%26dopt%3DAbstract Authors: Detorakis ET, Zaravinos A, Spandidos DA The expression of growth factors in ophthalmic pterygium and phenotypically normal conjunctiva was examined and correlated with the clinical findings. Fifteen specimens of ophthalmic pterygia and 8 specimens of phenotypically normal conjunctiva were examined. Total RNA was extracted from all specimens and mRNA levels for transforming growth factor (TGFB1), vascular endothelial growth factor (VEGFA), basic fibroblast growth factor (FGF2), epidermal growth factor (EGF) and insulin-like growth factor (IGF1) were measured using real-time reverse-transcription PCR (qRT-PCR). Differences in the expression of these factors between pterygium and conjunctival specimens were examined, as well as correlations between RNA levels and clinical paramet... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328201 Thu, 04 Mar 2010 13:22:58 +0100 3328201 http://www.medworm.com/index.php?rid=3328200&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198299%26dopt%3DAbstract In this study, we compared the radiosensitivity or radioresistance in two non-small cell lung cancers (NSCLCs), H460 and A549, and investigated the signaling pathways that confer radioresistance. H460 cells showed a significant G2/M arrest after 12 h of irradiation (5 Gy), reaching 60% of G2/M phase arrest. A549 cells also showed a significant G2/M arrest after 12 h of exposure; however, this arrest completely disappeared after 24 h of exposure. A549 has higher methylated CpG sites in PTEN, which is correlated with tumor radioresistance in some cancer cells, than H460 cells, and the average of the extent of the methylation was approximately 4.3 times higher in A549 cells than in H460 cells. As a result, PTEN expression was lower in A549 than in H460. Conducting Western blot analysis, we fo... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328200 Thu, 04 Mar 2010 13:22:55 +0100 3328200 http://www.medworm.com/index.php?rid=3328199&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198300%26dopt%3DAbstract Authors: Ozsvári B, Puskás LG, Nagy LI, Kanizsai I, Gyuris M, Madácsi R, Fehér LZ, Gerö D, Szabó C In recent years, a new cell-based high throughput paradigm has emerged, which seeks to identify novel, pharmacologically active cytoprotective compounds. The essence of this approach is to create experimental models of cell injury relevant for a particular disease by establishing in vitro cell-based models, followed by high-throughput testing of compounds that affect the cellular response in a desired manner. Prior approaches typically used simple end-point analyses. To assess the cytoprotective effects of novel drug candidates in real-time, we have applied a cell-microelectronic sensing technique (RT-CES), which measures changes in the impedance of individua... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328199 Thu, 04 Mar 2010 13:22:52 +0100 3328199 http://www.medworm.com/index.php?rid=3328198&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198301%26dopt%3DAbstract Authors: Dong XR, Luo M, Fan L, Zhang T, Liu L, Dong JH, Wu G Microglia, the resident immune cells of the central nervous system (CNS), are activated by various stimuli. Resting microglia are the basis of normal neurogenesis, while activated microglia may inhibit neurogenesis through the production of pro-inflammatory mediators and cytokines. Recent research suggests that microglia are activated by irradiation. This may play a role in radiation-induced brain injury (RIBI). DNA double-strand breaks (DSBs), the most deleterious form of DNA damage after ionizing radiation, may rapidly trigger the activation of the NF-kappaB pathway via p53-induced protein leading to the release of pro-inflammatory mediators and cytokines. Thus, a negative regulator of the NF-kappaB pathway that inhibits r... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328198 Thu, 04 Mar 2010 13:22:48 +0100 3328198 http://www.medworm.com/index.php?rid=3328197&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198302%26dopt%3DAbstract Authors: Futami K, Ogasawara S, Goto H, Yano H, Furuichi Y RecQL1 in the human RecQ DNA helicase family participates in DNA repair and recombination pathways in cell cycle replication. Immunohistochemical analysis of human hepatocellular carcinoma (HCC) tissues showed that RecQL1 expression is strongly correlated with histological grade and MIB-1 indices of HCC, and that the expression was greater in simple HCCs inducing extranodular growth or portal vein invasion than in HCCs not inducing extranodular growth or portal vein invasion. These histological data reveal the potential of RecQL1 as a biological marker predicting the malignancy and progression of liver cancer. High expression profiles were also produced by various HCC cells, including HCC cell lines established by us. When RecQ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328197 Thu, 04 Mar 2010 13:22:45 +0100 3328197 http://www.medworm.com/index.php?rid=3328196&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198303%26dopt%3DAbstract Authors: Kanauchi O, Mitsuyama K, Komiyama Y, Yagi M, Andoh A, Sata M Irritable bowel syndrome (IBS) is a common health issue that is characterized by abdominal pain, abnormal bowel movements, altered visceral perception, and abnormal metabolism of 5-hydroxy triptamine (serotonin; 5HT). The use of prebiotics or probiotics treatment for IBS has become increasingly important as an adjunct to pharmaceutical options. The aim of this study was to determine the efficacy of enzyme-treated rice fiber (ERF) on an IBS model. We obtained a new prebiotic from defatted rice bran that was developed as an insoluble dietary fiber through amylase and hemicellulase treatment followed by removal of the soluble fraction. Containing approximately 70% hemicellulose, ERF is utilized by lactobacilli and subse... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328196 Thu, 04 Mar 2010 13:22:42 +0100 3328196 http://www.medworm.com/index.php?rid=3328195&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198304%26dopt%3DAbstract Authors: Moosmeier MA, Bulkescher J, Hoppe-Seyler K, Hoppe-Seyler F The targeted inactivation of intracellular molecules has important therapeutic potential. For this purpose, it could be envisioned to introduce specifically designed binding proteins into cells by covalent linkage to protein transduction domains (PTDs). However, stable linkage of a PTD to a cargo may affect its conformation and, hence, its binding property inside the cell. Here, we analyzed the ability of non-covalently linked PTDs to internalize the model binding proteins streptavidin (SA) and Strep-Tactin (ST). Notably, inside the cell, the PTD-Strep-tag II ligand used for internalization of SA was displaced by the model target biotin which exhibits a higher binding affinity for the same binding pocket. Thus, specifi... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328195 Thu, 04 Mar 2010 13:22:40 +0100 3328195 http://www.medworm.com/index.php?rid=3328194&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198305%26dopt%3DAbstract Authors: Cai KM, Bao XL, Kong XH, Jinag W, Mao MR, Chu JS, Huang YJ, Zhao XJ MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively modulate gene expression at the post-transcriptional level. A growing number of studies has shown that more and more miRNAs are aberrantly expressed and involved in the pathogenesis of several types of cancers. Here, we report that the down-regulated hsa-miR-34c was also involved in oncogenesis of laryngeal carcinoma. Our studies indicated that hsa-miR-34c functioned as a tumor suppressor which inhibited growth and invasion of human laryngeal carcinoma cells. Furthermore, in our study, an inverse relationship between the expression of hsa-miR-34c and c-Met was identified in 10 paired fresh samples from tumor tissues and adjacent normal tissue... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328194 Thu, 04 Mar 2010 13:22:37 +0100 3328194 http://www.medworm.com/index.php?rid=3328193&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198306%26dopt%3DAbstract In conclusion, MLN64 is overexpressed in breast cancer, and its level correlates with poor prognosis and patient survival. MLN64 contributes to the development and progression of breast cancer through the regulation of cell proliferation and adhesive capacity. PMID: 20198306 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328193 Thu, 04 Mar 2010 13:22:34 +0100 3328193 http://www.medworm.com/index.php?rid=3328192&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198307%26dopt%3DAbstract In this study, we evaluated the functions of the V. vulnificus capsular polysaccharide on interleukin (IL)-8 production, as well as its underlying mechanisms in human intestinal epithelial cells. The CPS-defective wbpP mutant induced significantly lower levels of IL-8 production, IL-8 gene promoter activation and NF-kappaB activity in INT-407 cells than was noted with the wild-type or wbpP-complemented V. vulnificus. The expression levels of Toll-like receptor (TLR)2 mRNA and protein were also found to be lower in INT-407 cells infected with the CPS-defective wbpP mutant than in those cells infected with the wild-type or the wbpP-complemented strains. Additionally, the treatment of INT-407 cells with anti-TLR2 antibody proved to significantly block IL-8 production and NF-kappaB minimal pro... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328192 Thu, 04 Mar 2010 13:22:32 +0100 3328192 http://www.medworm.com/index.php?rid=3328191&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198308%26dopt%3DAbstract Authors: Takeda H, Inoue H, Kutsuna T, Matsushita N, Takahashi T, Watanabe S, Higashiyama S, Yamamoto H Chondrocytes produce many types of ECM to maintain elasticity and plasticity in articular cartilage of revolute joints. Both transforming growth factor beta (TGF-beta) and bone morphogenetic proteins (BMPs) induce extracellular matrix proteins such as type IIalpha1 collagen and aggrecan during chondrogenic differentiation in vitro. However, differences in the matrix gene expression pattern by the stimulation of TGF-betas and BMPs remains unclear. In the present study, we created a customized PCR-based ECM array to investigate the pattern of ECM expression genes in the chondrocyte progenitor cell line ATDC5, that was stimulated by TGF-betas or BMPs. Fibronectin (Fn) expression was dra... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328191 Thu, 04 Mar 2010 13:22:29 +0100 3328191 http://www.medworm.com/index.php?rid=3328190&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198309%26dopt%3DAbstract Authors: Ooi K, Sugimoto K, Shiraki K, Yamamoto N, Tameda M, Beppu T, Tanaka J, Nojiri K, Kusagawa S, Takei Y, Masuda C, Nobori T Patterns of hypocholesterolemic lipid fractions in 295 patients with liver diseases, malignant tumors, arteriosclerotic and renal diseases with cholesterol (Chol) levels of <30 mg/dl were classified using a simultaneous analytical method for the Chol and triglyceride (TG) fractions (Chol/Trig Combo System). Hypocholesterolemia was classified as follows: IV, Type IV on WHO hyperlipidemia phenotype classification; intermediate density lipoprotein (IDL), cases with appearance of IDL, including appearance of Lp(a); high + low density lipoproteins (HDL+LDL), lipids mostly consisting of HDL and LDL fractions; HDL abnormality, cases with slow alphaHDL or fast HD... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328190 Thu, 04 Mar 2010 13:22:26 +0100 3328190 http://www.medworm.com/index.php?rid=3328189&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198310%26dopt%3DAbstract Authors: Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y Dyslipidemia is an important risk factor for myocardial infarction (MI). We previously showed that gene polymorphisms associated with MI differed among individuals with different lipid profiles. We further examined whether genetic variants that confer susceptibility to MI might differ among individuals with low or high serum concentrations of triglycerides, high density lipoprotein (HDL)-cholesterol, or low density lipoprotein (LDL)-cholesterol. The study population comprised 5270 Japanese individuals, including 1188 subjects with MI and 4082 controls. The 150 polymorphisms examined in the present study were selected by genome-wide association studies of MI and ischemic ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328189 Thu, 04 Mar 2010 13:22:23 +0100 3328189 http://www.medworm.com/index.php?rid=3328188&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198311%26dopt%3DAbstract Authors: Ajtay Z, Németh A, Sulyok E, Cziráki A, Szabados S, Martens-Lobenhoffer J, Awiszus F, Szabó C, Bode-Böger SM The study was designed to compare the response pattern of plasma l-arginine and methylarginines to stent placement in patients with or without ST segment elevation myocardial infarction (STEMI). Two groups of patients with obstructive coronary artery disease (OCAD) undergoing percutaneous coronary intervention (PCI) with stenting were enrolled in the study. Group I consisted of 16 patients with STEMI, whereas group II included 24 patients without STEMI (controls). Before PCI and at <1 h, 5 and 30 days after reperfusion, blood samples were taken for measurement of l-arginine and methylarginines. L-arginine, asymmetric dimethylarginine (ADMA), symme... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328188 Thu, 04 Mar 2010 13:22:21 +0100 3328188 http://www.medworm.com/index.php?rid=3328187&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198312%26dopt%3DAbstract Authors: Auci DL, Mangano K, Destiche D, White SK, Huang Y, Boyle D, Frincke J, Reading CL, Nicoletti F HE3286 (17alpha-ethynyl-5-androstene-3beta, 7beta, 17beta-triol) is an orally bio-available synthetic derivative of naturally occurring androstene-3beta, 7beta, 17beta-triol. Our present data show that oral treatment with HE3286, favourably influenced the course of arthritis in the rat model of adjuvant-induced arthritis (reduced cumulative disease scores and paw edema), and in the mouse model of collagen antibody-induced arthritis (reduced clinical paw scores). Importantly, HE3286 was not immune suppressive in human mixed lymphocyte reaction or in animals challenged with Coxsackie B3 virus. HE3286 is currently in phase I/II clinical trials in rheumatoid arthritis and ulcerative coli... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328187 Thu, 04 Mar 2010 13:22:17 +0100 3328187 http://www.medworm.com/index.php?rid=3328186&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198313%26dopt%3DAbstract Authors: Kim WJ, Lee SJ, Choi YD, Moon SK Decursin, a pyranocoumarin isolated from the Korean Angelica gigas root, has demonstrated anti-cancer properties. In the present study, we found that decursin inhibited cell viability in cultured human urinary bladder cancer 235J cells and colon cancer HCT116 cells. The inhibited proliferation was due to apoptotic induction, because both cells treated with decursin dose-dependently showed a sub-G1 phase accumulation and an increased cytoplasmic DNA-histone complex. Cell death caused by decursin was also associated with the down-regulation of anti-apoptotic factor Bcl-2 and the up-regulation of pro-apoptotic molecules cytochrome c, caspase 3 and Bax. Treatment of both types of cancer cells with decursin resulted in G1-phase cell cycle arrest, as... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328186 Thu, 04 Mar 2010 13:22:14 +0100 3328186 http://www.medworm.com/index.php?rid=3328185&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198314%26dopt%3DAbstract This study analyzes the activity of the enzyme involved in the removal of these sugar residues, i.e. beta-N-acetylglucosaminidase (O-GlcNAcase) as well as the level of O-GlcNAc in benign and malignant thyroid lesions. Our results demonstrate increased activity of the enzyme in thyroid cancers in comparison to non-neoplastic lesions and adenomas. O-GlcNAc-modified proteins in thyroid cells have a predominantly nuclear distribution and are more abundant in non-neoplastic lesions than in tumors. Understanding the aberrant O-GlcNAc metabolism in thyroid cancer cells may be helpful for developing new diagnostic or treatment methods. PMID: 20198314 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328185 Thu, 04 Mar 2010 13:22:11 +0100 3328185 http://www.medworm.com/index.php?rid=3328184&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198315%26dopt%3DAbstract We examined an association of genetic variants with intracerebral or subarachnoid hemorrhage among Japanese individuals. The study population comprised 4,304 unrelated Japanese individuals, including 377 subjects with intracerebral hemorrhage, 205 subjects with subarachnoid hemorrhage, and 3,722 controls. The 150 polymorphisms examined in the present study were selected by genome-wide association studies of ischemic stroke and myocardial infarction with the use of the GeneChip Human Mapping 500K Array Set. The chi-square test, multivariable logistic regression analysis with adjustment for covariates, as well as a stepwise forward selection procedure revealed that the Cright curved arrow T polymorphism (rs1324694) of ERLIN1, the Cright curved arrow T polymorphism (rs12679196) of TRAPPC9, an... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328184 Thu, 04 Mar 2010 13:22:09 +0100 3328184 http://www.medworm.com/index.php?rid=3328183&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198316%26dopt%3DAbstract In conclusion, the changes of ROS and GSH by MG132, NAC, BSO or DDC were partially related to cell growth and death in the lung cancer cell lines Calu-6 and A549. PMID: 20198316 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3328183 Thu, 04 Mar 2010 13:22:06 +0100 3328183 http://www.medworm.com/index.php?rid=3236785&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127033%26dopt%3DAbstract Authors: Stover C Complement-dependent cytotoxicity is one of the mechanisms by which therapeutic monoclonal antibodies are successful against cancer. Complement is one of the innate immune defence systems, whose activation products and membrane-bound regulators interact with cells of the adaptive immune response. The complement system is currently undergoing a re-appreciation in its role within the immune surveillance of tumour. The majority of human tumours are low immunogenic. Complement may be involved through direct, 'danger signal'-elicited activation or via infiltration of inflammatory cells, which express complement components. Inflammatory cells may be associated with malignant transformation and tumour regression. The evidence for the effects of complement activation and regu... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236785 Thu, 04 Feb 2010 11:49:03 +0100 3236785 http://www.medworm.com/index.php?rid=3236784&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127034%26dopt%3DAbstract Authors: Choi SY, Lee KY, Kim YE, Bae JW, Oh SK, Kim SY, Hwang SJ, Kim UK, Lee SH Congenital hearing loss (HL) is the most common sensory disorder in humans, affecting one in 1000 infants at birth. A high degree of genetic heterogeneity makes it difficult to screen for mutations in all known deafness genes in clinical applications. We have improved a genotyping microarray using the multiplex PCR-based allele-specific primer extension (ASPE) reaction and applied this method for the genetic diagnosis of congenital HL in Korea. Seven different mutations in the GJB2, SLC26A4 and mitochondrial 12S rRNA genes, which were identified on the basis of a previous study in a Korean population, were selected for the study. These genes were used to evaluate the accuracy of the microarray. The test f... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236784 Thu, 04 Feb 2010 11:49:01 +0100 3236784 http://www.medworm.com/index.php?rid=3236783&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127035%26dopt%3DAbstract Authors: Witkowska A, Gumprecht J, Glogowska-Ligus J, Wystrychowski G, Owczarek A, Stachowicz M, Bocianowska A, Nowakowska-Zajdel E, Mazurek U Cancer is a disease of genomic instability, a multistep process involving numerous mutations and chromosomal aberrations. Telomeres are highly specialized structures at the ends of chromosomes and function to stabilize and protect the ends of linear chromosomes, therefore determining cellular immortalization. Homeostasis of telomere length is a multifactor-dependent process. Since cellular immortalization is an early and essential step towards cancer, the aim of the present study was to determine immortalization genes that are significant in colon cancer and assess their usefulness in the early diagnosis of this tumor. Expression profiles of 119... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236783 Thu, 04 Feb 2010 11:48:58 +0100 3236783 http://www.medworm.com/index.php?rid=3236782&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127036%26dopt%3DAbstract Authors: Tanabe S, Hochi S Anti-allergic effects of galactooligosaccharide (GOS), which is found in breast milk and frequently added to food for promoting health, were evaluated in a human-like mouse model of atopic dermatitis (AD). NC/Nga mice were fed 5.5% GOS for 8 weeks, and we examined whether this treatment suppressed the development of AD-like skin lesions in these mice. Mice fed GOS exhibited significantly less symptoms of dermatitis, reduced scratching frequency, and lower levels of serum total immunoglobulin E compared to control. At the end of the 8-week-experimental period, spleens were removed, and the splenocytes were stimulated with phorbol 12-myristate 13-acetate and ionomycin, following which production of cytokines and a chemokine was analyzed. Elevated levels of Th1 ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236782 Thu, 04 Feb 2010 11:48:55 +0100 3236782 http://www.medworm.com/index.php?rid=3236781&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127037%26dopt%3DAbstract Authors: Cho JY, Um HS, Kang EB, Cho IH, Kim CH, Cho JS, Hwang DY Exercise training was suggested as a practical therapeutic strategy for human subjects suffering from Alzheimer's disease (AD) in our previous study. Therefore, the purpose of this study was to investigate the effects of combining exercise training with the administration of antioxidants on the pathological phenotype of AD. To accomplish this, non-transgenic mice (Non-Tg) and NSE/APPsw Tg mice were treated with alpha-lipoic acid and treadmill exercised for 16 weeks, after which their brains were evaluated to determine whether any changes in the pathological phenotype-related factors occurred. The results indicated that (i) the combination-applied (COMA) Tg group with exercise training (ET) and alpha-lipoic acid administr... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236781 Thu, 04 Feb 2010 11:48:53 +0100 3236781 http://www.medworm.com/index.php?rid=3236780&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127038%26dopt%3DAbstract In this study we investigated the effect both of onion extract and quercetin on the proliferation of fibroblasts, expression of type I collagen and matrix metalloproteinase-1 (MMP-1). Our data show that proliferation rates of fibroblasts were decreased in a dose-dependent manner of the onion extract and quercetin. The expression of type I collagen was not markedly changed by the onion extract and quercetin. Interestingly, the expression of MMP-1 was markedly increased by both onion extract and quercetin in vitro and in vivo. Thus, our data indicate that onion extract and quercetin play a role in the anti-scar effect in skin through up-regulation of MMP-1 expression, implying this agent is a promising material for reducing scar formation. PMID: 20127038 [PubMed - in process] (Source: In... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236780 Thu, 04 Feb 2010 11:48:50 +0100 3236780 http://www.medworm.com/index.php?rid=3236779&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127039%26dopt%3DAbstract We examined the SG subunit expression in colonic aganglionic and ganglionic specimens obtained from patients with HD. Full-thickness bowel specimens were obtained from six patients with HD. Six normal colon specimens were used as controls. Immunofluorescent analysis and reverse transcriptase polymerase chain reaction evaluation were performed for alpha-, beta-, gamma-, delta- and epsilon-SG. In control colon, the indirect immunofluorescence showed a strong staining pattern of beta- gamma- delta- and epsilon-SG while a weak positivity of alpha-SG was recorded. In aganglionic bowel, immunofluorescence intensity values documented a significant lack of epsilon-SG while an enhanced alpha-SG, coupled to a loss of epsilon-SG, was recorded in ganglionic bowel in HD-affected patients. Our observati... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236779 Thu, 04 Feb 2010 11:48:47 +0100 3236779 http://www.medworm.com/index.php?rid=3236778&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127040%26dopt%3DAbstract This study was designed to identify the biological characteristics of the STEAP4 gene in human adipocytes. On the basis of oil red O staining and the expression profiles of specific markers, we demonstrated that overexpression of STEAP4 did not affect adipogenesis. 2-Deoxy-D-[3H]-glucose uptake tests showed that STEAP4 promoted insulin-stimulated glucose uptake in mature human adipocytes. Further data from quantitative real-time RT-PCR and Western blotting revealed that the adipokines tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6), and leptin, which have been implicated in insulin sensitivity regulation, regulate the expression of STEAP4. Our results demonstrate that STEAP4 does not influence human adipocyte differentiation, but it participates in regulating the insulin sensi... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236778 Thu, 04 Feb 2010 11:48:44 +0100 3236778 http://www.medworm.com/index.php?rid=3236777&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127041%26dopt%3DAbstract Authors: Oh JY, Park MY, Kim DR, Lee JH, Shim SH, Chung JH, Yoon HI, Lee JH, Sung MW, Kim YS, Lee CT A major obstacle to the success of gene therapy strategies that directly target cancer cells is the low gene transfer rate. To address this problem, we had previously proposed a combination adenoviral gene therapy containing a conditionally replicating adenovirus (CRAD) expressing mutant E1 (Delta24RGD), and a replication-defective E1-deleted adenovirus to enhance the efficiency of gene transfer. Suicide/pro-drug gene therapy has an important additional benefit to the therapy of cancer. This relates to the transfer and expression of non-mammalian genes encoding enzymes that convert non-toxic pro-drugs into cellular toxins. We investigated the interaction between CRAD (Delta24RGD) and a ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236777 Thu, 04 Feb 2010 11:48:41 +0100 3236777 http://www.medworm.com/index.php?rid=3236776&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127042%26dopt%3DAbstract In this study, we cultured human MSCs and chondrogenic differentiation MSCs of 3 donors, and investigated the miRNA expression profiles of MSCs and chondrogenic differentiated MSCs from 2 donors by using miRNA microarrays. We found 5 miRNAs were significantly overexpressed in chondrogenic differentiated MSCs in each sample, and 4 were further confirmed by real-time RT-PCR assay in the sample from the third independent donor. We also predicted the confirmed miRNAs corresponding to putative target genes by online software. The results indicated that the overexpressed miRNAs in chondrogenic differentiated MSCs may play a role in the procedure of MSC chondrogenesis, which offers us guidance for further studies. PMID: 20127042 [PubMed - in process] (Source: International Journal of Molecula... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236776 Thu, 04 Feb 2010 11:48:39 +0100 3236776 http://www.medworm.com/index.php?rid=3236775&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127043%26dopt%3DAbstract Authors: Hu XS, Du CQ, Yang L, Yao XY, Hu SJ The aim of this study was to determine whether proteasome inhibitor MG132 treatment has any effect on endothelial progenitor cells (EPCs). Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation. EPCs were identified as adherent cells double positive for DiLDL-up-take and lectin binding by direct fluorescent demonstrated under a laser scanning confocal microscope. After 7 days in culture, EPCs were stimulated with proteasome inhibitor MG132 in series of final concentrations of 20, 50, 100, 200 nmol/l for 12, 24, 48 h. Cell proliferation and apoptosis were determined, respectively, by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, annexin V/propidium iodide bindin... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236775 Thu, 04 Feb 2010 11:48:36 +0100 3236775 http://www.medworm.com/index.php?rid=3236774&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127044%26dopt%3DAbstract In conclusion, millimeter wave treatment inhibits the SNP-induced apoptosis of chondrocytes through the p38MAPK pathway. PMID: 20127044 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236774 Thu, 04 Feb 2010 11:48:33 +0100 3236774 http://www.medworm.com/index.php?rid=3236773&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127045%26dopt%3DAbstract Authors: Zou L, Yang R, Zhang P, Dai Y Aging is considered a high risk factor for Alzheimer's disease (AD), which is one of the most prevalent neurodegenerative disorders in the elderly population. The major pathologic feature of AD is senile plaques mainly containing amyloid-beta (Abeta) components. However, little direct evidence has shown aging in association with Abeta. Here we show that the protein-protein interaction of amyloid precursor protein (APP) and beta -site amyloid cleavage enzyme 1 (BACE1) is enhanced by the fluorescence resonance energy transfer (FRET) assay during the aging process, and the APP-BACE1 complex accumulates in the endosome in the IMR-90 fibroblast (NHF) cellular aging models. Moreover, enhanced Abeta is observed in aged cells, rat brain homogenates and hu... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236773 Thu, 04 Feb 2010 11:48:30 +0100 3236773 http://www.medworm.com/index.php?rid=3236772&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127046%26dopt%3DAbstract Authors: Heo JC, Park CH, Lee HJ, Kim SO, Kim TH, Lee SH Inflammation of the respiratory tract is a crucial process in immune diseases, including asthma, and atopic rhinitis. To establish whether an aqueous extract of Spinacia oleracea Linn (SoL) has a beneficial influence in terms of anti-asthmatic activity, we examined its effects on an ovalbumin-induced asthmatic model. Mice sensitized to ovalbumin were orally administered the SoL extract, and their lungs examined by hematoxylin and eosin staining to determine IL-4/13 cytokine expression. The SoL extract exerted strong anti-asthmatic effects by inducing a decrease in the CD4+ cell number, IL-4/13, and other molecular markers in the lung. Our results collectively indicate that the aqueous SoL extract ameliorates asthmatic symptoms ef... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236772 Thu, 04 Feb 2010 11:48:27 +0100 3236772 http://www.medworm.com/index.php?rid=3236771&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127047%26dopt%3DAbstract Authors: Tomita M, Katsuyama H, Okuyama T, Watanabe Y, Hidaka K, Otsuki T, Nata M Exposure to stress activates the hypothalamus-pituitary-adrenal (HPA) axis, which is followed by an increase in production of its end product, corticosterone, considered to be the most important glucocorticoid (GC) in rodents. Glucocorticoid receptor (GR) signaling has been suggested as a potential mechanism responsible for the pathogenesis of many clinical disorders. Here, we investigated the involvement of the GR polymorphism in stress response. ICR mice were screened by genomic PCR, bred, and divided into 3 groups according to the GR polymorphism, GRwt/wt, GRwt/Qn, and GRQn/Qn. Mice were exposed to water-immersion restraint stress (WRS), and then examined for gastric mucosal lesions, serum corticostero... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236771 Thu, 04 Feb 2010 11:48:24 +0100 3236771 http://www.medworm.com/index.php?rid=3236770&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127048%26dopt%3DAbstract Authors: Ruimi N, Rwashdeh H, Wasser S, Konkimalla B, Efferth T, Borgatti M, Gambari R, Mahajna J Nitric oxide (NO) is a radical molecule produced by iNOS and plays a role in various physiological and pathophysiological conditions including inflammatory diseases and cancer. In the present study, organic extract of Daedalea gibbosa was effective in inhibiting NO and PGE2 production in RAW 264.7 cells. The extract of D. gibbosa was chemically fractionated leading to the isolation of three active fractions (F5-F7) that were effective in inhibiting NO and iNOS production. In addition, F6 and F7 significantly inhibited the iNOS transcript, while F5 did not cause a reduction in the iNOS transcript. Furthermore, the active fractions showed a differential effect on levels of phospho-p38, phosp... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236770 Thu, 04 Feb 2010 11:48:22 +0100 3236770 http://www.medworm.com/index.php?rid=3236769&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127049%26dopt%3DAbstract Authors: Tsujimoto K, Uozaki M, Ikeda K, Yamazaki H, Utsunomiya H, Ichinose M, Koyama AH, Arakawa T Viral clearance is a primary concern for parenteral protein biopharmaceuticals. Low pH, detergent/solvent wash, or heating, called pasteurization, has been the main process for virus inactivation. Detergent/solvent wash is also used to treat superficial infectious diseases, including herpes simplex virus (HSV) infections. Herein we examined virus inactivation effects of acidic arginine on HSV type 2 (HSV-2) as a function of pH and temperature in an attempt to find solvent conditions that are effective for virus inactivation, yet are compatible with in vivo applications. Aqueous arginine at 0.7 M was highly effective on HSV-2, more so at lower pH and higher temperature. Its effects were s... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236769 Thu, 04 Feb 2010 11:48:19 +0100 3236769 http://www.medworm.com/index.php?rid=3236768&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127050%26dopt%3DAbstract In this study, we investigated the role of the KXGFFKR motif in the adhesion of leukocytes to human umbilical-vein endothelial cells (HUVEC). Pre-treatment of human neutrophils with a membrane-permeable peptide-linked KVGFFKR decreased cell adhesion to HUVEC induced by a complement activation product, C5a and formyl-methionine-leucine-phenylalanine. Similar inhibitory efficacies of this peptide were observed in T cell adhesion. Our data therefore demonstrate that a highly conserved sequence in the alpha integrins, KXGFFKR, is pharmacologically essential for integrin activation during leukocyte adhesion by both neutrophils and T cells. PMID: 20127050 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236768 Thu, 04 Feb 2010 11:48:16 +0100 3236768 http://www.medworm.com/index.php?rid=3236767&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127051%26dopt%3DAbstract Authors: Mullen E, Ohlendieck K Abnormal glucose handling has emerged as a major clinical problem in millions of diabetic patients worldwide. Insulin resistance affects especially one of the main target organs of this hormone, the skeletal musculature, making impaired glucose metabolism in contractile fibres a major feature of type 2 diabetes. High levels of circulating free fatty acids, an increased intramyocellular lipid content, impaired insulin-mediated glucose uptake, diminished mitochondrial functioning and an overall weakened metabolic flexibility are pathobiochemical hallmarks of diabetic skeletal muscles. In order to increase our cellular understanding of the molecular mechanisms that underlie this complex diabetes-associated skeletal muscle pathology, we initiated herein a ma... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236767 Thu, 04 Feb 2010 11:48:14 +0100 3236767 http://www.medworm.com/index.php?rid=3236766&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127052%26dopt%3DAbstract Authors: Tsujimoto K, Sakuma C, Uozaki M, Yamasaki H, Utsunomiya H, Oka K, Koyama AH N-methyl-pyridinium formate, a novel component of coffee extracts, inhibited the multiplication of both DNA and RNA viruses. In the presence of the compound, the progeny viral yields of both herpes simplex virus type 1 (HSV-1) and poliovirus in HEp-2 cells and those of influenza virus type A in MDCK cells decreased with increasing concentrations of the compound, although the degree of viral sensitivity to this compound differed. In addition, none of these viruses were directly inactivated by the compound at the concentrations tested. Characterization of the mode of action of this compound against HSV-1 multiplication revealed that it inhibits the viral growth primarily at the initial step of virus mult... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236766 Thu, 04 Feb 2010 11:48:11 +0100 3236766 http://www.medworm.com/index.php?rid=3236765&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127053%26dopt%3DAbstract Authors: Davenport A, Frezza M, Shen M, Ge Y, Huo C, Chan TH, Dou QP Chemotherapy remains the staple of treatment for many types of leukemia. Despite the positive impact on extending overall survival in patients with hematological malignancies, new treatment strategies are needed to reduce the nonspecific toxicity and improve the efficacy of treatment. Celastrol, derived from the 'Thunder God Vine' and Pro-EGCG, a pre-drug version of green tea polyphenol EGCG have shown potent biological activity in vitro and in vivo. Whether these natural products augment the efficacy of conventional chemotherapy in the treatment of leukemia cells has yet to be demonstrated. Here we demonstrate that these natural products could sensitize the effect of chemotherapy in both K-562 and Jurkat T human leuk... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236765 Thu, 04 Feb 2010 11:48:08 +0100 3236765 http://www.medworm.com/index.php?rid=3236764&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127054%26dopt%3DAbstract Authors: Tahara T, Shibata T, Nakamura M, Yamashita H, Yoshioka D, Okubo M, Yonemura J, Maeda Y, Maruyama N, Kamano T, Kamiya Y, Fujita H, Nakagawa Y, Nagasaka M, Iwata M, Hirata I, Arisawa T CpG island hyper-methylation (CIHM) is one of the major events in the gastric carcinogenesis. IL-17A, -17F and MIF have a crucial role in the gastric inflammation and carcinogenesis. Recently, we showed that the genetic polymorphisms of MIF-794-CATT repeat are associated with CIHM status in the non-neoplastic gastric mucosa. Consequently, the CIHM status in the gastric cancer tissue, in relation to IL-17A (-197G>A), -17F (7488T>C), and MIF (-173G>C and -794 tetranucleotide repeats) polymorphisms was investigated. Gastric cancer tissues were obtained from 102 patients. CIHM of p14, p16, DA... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3236764 Thu, 04 Feb 2010 11:48:05 +0100 3236764 http://www.medworm.com/index.php?rid=3135888&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043126%26dopt%3DAbstract We examined the long-term (24 weeks) effect of this diet on the liver of Zucker (fa/fa) rats. The synthetic diet-fed Zucker (fa/fa) rats showed hepatic fibrosis during the development of NAFLD with no apparent infiltration of inflammatory cells. They showed significantly elevated hepatic mRNA levels of proinflammatory and profibrogenic cytokines. These findings suggest that excess long-term feeding of a diet similar to the synthetic diet used in our study leads to hepatic fibrosis during the development of NAFLD in patients with obesity or type 2 diabetes. Our results were different from NAFLD accompanied by infiltration of inflammatory cells. However, they also suggest that long-term feeding of this synthetic diet to Zucker (fa/fa) rats is useful for studying the hepatic fibrogenesis duri... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135888 Fri, 01 Jan 2010 22:58:56 +0100 3135888 http://www.medworm.com/index.php?rid=3135887&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043127%26dopt%3DAbstract Authors: De Carlo E, Baiguera S, Conconi MT, Vigolo S, Grandi C, Lora S, Martini C, Maffei P, Tamagno G, Vettor R, Sicolo N, Parnigotto PP Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matr... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135887 Fri, 01 Jan 2010 22:58:53 +0100 3135887 http://www.medworm.com/index.php?rid=3135886&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043128%26dopt%3DAbstract Authors: Araki Y, Mukaisho K, Sugihara H, Fujiyama Y, Hattori T The pathogenic mechanisms responsible for inflammatory bowel disease, especially ulcerative colitis (UC), are poorly understood. As an animal model, the oral administration of dextran sulfate sodium (DSS) induces colitis, which exhibits several clinical and histological features similar to UC. However, the pathogenic factors responsible for DSS-induced colitis and above all, the intestinal microflora in this colitis remain unclear. Therefore, we investigated the relationships between DSS and the intestinal microflora in this study. First, the depolymerization of DSS in mouse feces was analyzed using a pyridylamino-labeling (PA-DSS) and HPLC system. Next, a bacteriological study of the fecal contents using DSS-rich media an... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135886 Fri, 01 Jan 2010 22:58:50 +0100 3135886 http://www.medworm.com/index.php?rid=3135885&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043129%26dopt%3DAbstract In conclusion, the establishment of breast cancer cells in the peritoneum was inhibited by the transplantation of antibody-expressing cells. Since this method requires cell transplantation only once, the drug cost may be reduced. PMID: 20043129 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135885 Fri, 01 Jan 2010 22:58:47 +0100 3135885 http://www.medworm.com/index.php?rid=3135884&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043130%26dopt%3DAbstract This study indicates that there is no difference in the biomechanical outcome of conservative versus operative AT rupture treatment and only a marginal impact of short-term combinatory growth and angiogenesis factor application. PMID: 20043130 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135884 Fri, 01 Jan 2010 22:58:44 +0100 3135884 http://www.medworm.com/index.php?rid=3135883&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043131%26dopt%3DAbstract Authors: Yukawa K, Tanaka T, Yoshida K, Takeuchi N, Ito T, Takamatsu H, Kikutani H, Kumanogoh A Semaphorins are a family of secreted and membrane-bound proteins known as axonal pathfinders. Sema4A, a member of class 4 semaphorins, induces growth cone collapse of hippocampal neurons. The binding of Sema4A to growth cones indicates the presence of receptors transmitting signals through the intracellular effectors to induce growth cone collapse in hippocampal neurons. Transfection experiments of the candidate receptor genes into COS-7 cells demonstrated that Sema4A binds to axonal guidance receptors Plexin-B1, -B2 and -B3. To identify the functional Sema4A receptor and the signal transduction machinery, COS-7 cell contraction assay was performed, in which intracellular signal transmission... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135883 Fri, 01 Jan 2010 22:58:41 +0100 3135883 http://www.medworm.com/index.php?rid=3135882&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043132%26dopt%3DAbstract Authors: Chiu TL, Su CC Tanshinone IIA (Tan-IIA) is extracted from Danshen and known to inhibit proliferation and induce apoptosis in many cancer cells. We aimed to elucidate its anticancer activity and molecular mechanism in human lung cancer A549 cells. The cytotoxicity of Tan-IIA in A549 cells were measured by the MTT assay. The effects of Tan-IIA on the cell cycle, mitochondrial membrane potential (MMP), calcium and reactive oxygen species (ROS) released in A549 cells were detected by flow cytometry. The protein expressions of p53, Bax, Bcl-2 and beta-actin in A549 cells were tested by Western blotting. The proliferative rates of A549 cells were obviously inhibited by Tan-IIA in a dose- and time-dependent manner. The results of FACS showed that the sub-G1 phase was increased when A... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135882 Fri, 01 Jan 2010 22:58:38 +0100 3135882 http://www.medworm.com/index.php?rid=3135881&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043133%26dopt%3DAbstract Authors: Li XL, Li BY, Gao HQ, Cheng M, Xu L, Li XH, Zhang WD, Hu JW Arterial remodeling is the change in structural properties of vessel in diabetes mellitus and contributes to the development of vascular complications. To prevent this development and to improve diabetic vascular complications, effective therapies directed toward the key molecular target are required. Grape seed proanthocyanidin extracts (GSPE) have been reported to be effective in treating arteriosclerosis, while little is known about the functional protein changes. We used streptozocin to induce diabetic rats. GSPE (250 mg/kg body weight/day) were administrated to diabetic rats for 24 weeks. Consequently, 2-D difference gel electrophoresis and mass spectrometry were used to investigate aortic protein profiles among ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135881 Fri, 01 Jan 2010 22:58:34 +0100 3135881 http://www.medworm.com/index.php?rid=3135880&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043134%26dopt%3DAbstract Authors: Kim HJ, Kazi JU, Lee YR, Nguyen DH, Lee HB, Shin JH, Soh JW, Kim EK Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135880 Fri, 01 Jan 2010 22:58:31 +0100 3135880 http://www.medworm.com/index.php?rid=3135879&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043135%26dopt%3DAbstract Authors: Noh EM, Youn HJ, Jung SH, Han JH, Jeong YJ, Chung EY, Jung JY, Kim BS, Lee SH, Lee YR, Kim JS Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in breast cancer cell invasion. NF-kappaB and AP-1 are known to induce MMP-9 expression. We investigated whether cordycepin, an NF-kappaB or AP-1 inhibitor, can modulate MMP-9 expression and cell invasion in MCF-7 cells. Toxicity of cordycepin was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MMP-9 expression was determined by real-time PCR, Zymography, and Western blot analysis. AP-1 activation was assayed by electrophoretic mobility shift assay (EMSA). MAPK signaling was evaluated by Western blotting with specific p-ERK, and ERK, p-p38... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135879 Fri, 01 Jan 2010 22:58:28 +0100 3135879 http://www.medworm.com/index.php?rid=3135878&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043136%26dopt%3DAbstract In this study, 21 healthy volunteers underwent PET imaging with 11C Altropane before and after administration of oral doses of DBDS-MPH and OROS-MPH. Each subject received 40 mg DBDS-MPH and 36 mg OROS-MPH on different days. PET imaging occurred at 10 h after dosing. Each subject was injected with 5 mCi of 11C Altropane and serial images of the brain were acquired over 60 min with a Siemens HR+ PET camera. Binding potentials (BP, k3/k4) were calculated from time-activity curves using the simplified reference region method with cerebellum as reference. Transporter occupancy was calculated by standard methods. At 10 h, plasma d-MPH levels were lower (3.8+/-1.2 vs. 5.2+/-2.0) and brain DAT occupancy was lower (34.8+/-12.9 vs. 44.3+/-11.8) for DBDS-MPH than OROS-MPH. Across the range of values... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135878 Fri, 01 Jan 2010 22:58:25 +0100 3135878 http://www.medworm.com/index.php?rid=3135877&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043137%26dopt%3DAbstract Authors: Hirata Y, Ito H, Furuta T, Ikuta K, Sakudo A There is a limited number of reports regarding detergents and proteases inactivating, degrading, or destabilizing abnormal prion protein (PrPSc). In the present study, the effect of alkaline detergents and proteases on the breakdown of PrPSc in the absence of proteinase K (PK) (degradation) and the presence of PK (destabilization) was investigated. PrPSc from brain homogenate of terminally-diseased mice infected with the Chandler strain of scrapie was used as a substrate. A surfactant-free alkaline detergent (pH 11.9, 1% aqueous solution) with potassium hydroxide as the main ingredient and an alkaline detergent (pH 11.9, 1% aqueous solution) containing about 1% surfactant as well as two commercially available alkaline proteases had ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135877 Fri, 01 Jan 2010 22:58:22 +0100 3135877 http://www.medworm.com/index.php?rid=3135876&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043138%26dopt%3DAbstract Authors: Redova M, Chlapek P, Loja T, Zitterbart K, Hermanova M, Sterba J, Veselska R We investigated the possible modulation by LOX/ COX inhibitors of all-trans retinoic acid (ATRA)-induced cell differentiation in two established neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2). Caffeic acid, as an inhibitor of 5-lipoxygenase, and celecoxib, as an inhibitor of cyclooxygenase-2, were chosen for this study. The effects of the combined treatment with ATRA and LOX/COX inhibitors on neuroblastoma cells were studied using cell morphology assessment, detection of differentiation markers by immunoblotting, measurement of proliferation activity, and cell cycle analysis and apoptosis detection by flow cytometry. The results clearly demonstrated the potential of caffeic acid to enhance ATRA-indu... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135876 Fri, 01 Jan 2010 22:58:19 +0100 3135876 http://www.medworm.com/index.php?rid=3135875&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043139%26dopt%3DAbstract We examined an association of genetic variants with ischemic stroke among individuals with or without metabolic syndrome. The study population comprised 4,387 unrelated Japanese individuals, including 1,884 individuals with metabolic syndrome (240 subjects with ischemic stroke and 1,644 controls) and 2,503 individuals without metabolic syndrome (280 subjects with ischemic stroke and 2,223 controls). The 150 polymorphisms examined in the present study were selected by genome-wide association studies of ischemic stroke and myocardial infarction with the use of the GeneChip Human Mapping 500K Array Set (Affymetrix). The initial chi-square test revealed that the Cright curved arrow T polymorphism (rs9925481) of CLEC16A and the Aright curved arrow G polymorphism (rs4923918) of SPTBN5 were signi... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135875 Fri, 01 Jan 2010 22:58:15 +0100 3135875 http://www.medworm.com/index.php?rid=3135874&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043140%26dopt%3DAbstract Authors: Jang JY, Lee JH, Shin HK, Choi YH, Lee JD, Choi BT The active fraction of the extract of Asiasari radix, the 60% methanol chromatographic fraction from the ethyl acetate layer (PPAR), was used to investigate the melanogenesis signal pathway in B16F10 melanoma cells. PPAR led to significantly decreased melanin synthesis and tyrosinase activity in a dose-dependent manner. PPAR also reduced the expression of melanogenesis-related proteins including microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein (TRP)s while down-regulating the expression of mRNA of MITF and tyrosinase. Melanogenesis-regulating signals, such as mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase (PI3K)/... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135874 Fri, 01 Jan 2010 22:58:12 +0100 3135874 http://www.medworm.com/index.php?rid=3135873&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043141%26dopt%3DAbstract Authors: Choi EJ, Lee JI, Kim GH We investigated the antiproliferative effects of synthetic flavanone derivatives using an MTT assay in MCF-7 and MDA-MB-453 cells. When cells were treated with synthetic flavanone derivatives in concentrations ranging from 1 to 200 microM for 48 h, cell growth decreased at concentrations >50 microM. 4'-Chloroflavanone is more potent than flavanone among the synthetic flavanone derivatives. Exposure to 4'-chloroflavanone at 50 microM for 48 h caused cell cycle arrest in both MCF-7 and MDA-MB-453 cells. In addition, when 4'-chloroflavanone caused G1/S phase arrest, a decrease in CDK4 and cyclin D, together with an increase in p21Cip1, was observed in the cells. The p21Cip1 is a downstream target of p53 that may be affected by the activation of p53 by 4... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135873 Fri, 01 Jan 2010 22:58:08 +0100 3135873 http://www.medworm.com/index.php?rid=3135872&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043142%26dopt%3DAbstract Authors: Yang L, Wei J, He S Interferon (IFN)-lambdas, including IFN-lambda1, IFN-lambda2, and IFN-lambda3, are a newly described group of cytokines distantly related to the type I IFNs and IL-10 family members. Besides the antiviral activity, IFN-lambdas were reported to inhibit various tumor growths in vitro and in vivo. Herein, we identified IFN-lambda genes from the genome sequences of the human, chimpanzee, macaque, orangutan, mouse, rat and dog, and found that the locations and copy of a specific IFN-lambda varied in different genomes, not just the copy of IFN-lambdas. We found human IFN-lambdas were expressed in fetal retina, fetal brain and T cells by ESTs search. Moreover, IFN-lambdas were also found to express in bladder cancer, blood cancer, breast cancer, glioma, head and n... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3135872 Fri, 01 Jan 2010 22:58:05 +0100 3135872 http://www.medworm.com/index.php?rid=3057349&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956895%26dopt%3DAbstract Authors: Fukazawa T, Matsuoka J, Yamatsuji T, Maeda Y, Durbin ML, Naomoto Y Gene therapy and virotherapy are among the approaches currently used to treat malignant tumors. Gene therapy and virotherapy use a specific therapeutic gene that causes death in cancer cells. In early attempts at gene therapy, therapeutic genes were driven by ubiquitous promoters such as the CMV promoter, which induce non-specific toxicity to normal cells and tissues in addition to the cancer cells. Recently, novel cancer- and/or tissue-specific promoter systems have been developed to target cancer cells but not normal cells including stem cells. In this review, we describe cancer and/or tissue-specific gene therapy systems for the treatment of cancer. In particular, we will discuss three systems for gene thera... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057349 Sat, 05 Dec 2009 03:59:11 +0100 3057349 http://www.medworm.com/index.php?rid=3057348&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956896%26dopt%3DAbstract In this study, to elucidate the role of LMP2A in the tumorigenesis of EBVaGC, we established permanent cell lines expressing LMP2A. The LMP2A gene was cloned from a naturally EBV-infected EBVaGC cell line, SNU-719 and transduced into an EBV-negative GC cell line, AGS, using a retroviral vector. The sequence of SNU-719 LMP2A showed several conserved variations compared to that of the prototype EBV strain B95-8 LMP2A. Four of seven established cell clones expressed LMP2A protein at detectable levels. These cell clones did not show enhanced cell growth compared to control cells in normal or low serum-containing medium. Furthermore, LMP2A expression had no effect on colony forming ability of the cell clones in soft agar. Our results suggest that LMP2A alone has little effect on tumorigenesis o... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057348 Sat, 05 Dec 2009 03:59:09 +0100 3057348 http://www.medworm.com/index.php?rid=3057347&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956897%26dopt%3DAbstract In conclusion, these results suggest that activin A, but not inhibins, is produced by ovarian endometriosis and the normal endometrium, and that the activin signal transduction system exists in both ovarian endometriosis and the normal endometrium. PMID: 19956897 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057347 Sat, 05 Dec 2009 03:59:06 +0100 3057347 http://www.medworm.com/index.php?rid=3057346&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956898%26dopt%3DAbstract Authors: Kuribayashi T, Ohara M, Sora S, Kubota N A group of histone deacetylase (HDAC) inhibitors has been shown to suppress the growth of a variety of human tumor lines in vitro and in vivo and they are among the most promising candidates for anti-cancer therapeutic agents. We investigated the ability of scriptaid, a novel HDAC inhibitor and trichostatin A (TSA) to enhance cell killing by radiation in radioresistant SQ-20B cells derived from human head and neck squamous carcinoma. SQ-20B cells were treated with scriptaid or TSA in combination with radiation. Cell survival was determined by a colony formation assay and protein levels were examined by Western blotting. DNA double strand breaks were measured by a gamma-H2AX focus assay. Radiosensitization was observed for SQ-20B cells i... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057346 Sat, 05 Dec 2009 03:59:03 +0100 3057346 http://www.medworm.com/index.php?rid=3057345&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956899%26dopt%3DAbstract Authors: Jeong SY, Han MH, Jin CY, Kim GY, Choi BT, Nam TJ, Kim SK, Choi YH We isolated 23 marine actinomycetes from seawater samples. Of these, strain SY-103 exhibited the strongest cytotoxic activity on human leukemic cell lines. Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify SY-103 as a strain of the genus Streptomyces. In the present study, the pure cytotoxic compound (PCC) from Streptomyces sp. SY-103 metabolites was purified by reverse-phase HPLC and the biochemical mechanisms of PCC-induced apoptosis in cultured human leukemic cell lines were investigated. The exposure of cells to PCC resulted in growth inhibition and induction of apoptosis, which was associated with the proteolytic activation of caspase-3 and down-regulation of anti-apoptotic Bc... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057345 Sat, 05 Dec 2009 03:59:00 +0100 3057345 http://www.medworm.com/index.php?rid=3057344&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956900%26dopt%3DAbstract In this study, we showed that novel CPPs could be acquired by screening random peptides and modifying some amino acids could increase their cell-penetrating activity. PMID: 19956900 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057344 Sat, 05 Dec 2009 03:58:57 +0100 3057344 http://www.medworm.com/index.php?rid=3057343&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956901%26dopt%3DAbstract In this study, preventive effects of fermented ginseng (FG) against streptozotocin (STZ)-induced pancreatic beta-cell death was assessed in RINm5F insulinoma cells. FG markedly inhibited the production of nitrite in a dose-dependent manner. The decrease in nitrite production was found to correlate with reduced inducible nitric oxide (iNOS) protein and mRNA levels. To characterize the anti-inflammatory mechanism of FG at the transcriptional level, we examined effects of FG on the activity of nuclear factor-kappaB (NF-kappaB). FG reduced a translocation of the NF-kappaB subunit and NF-kappaB-dependent transcriptional activity. FG blocked signaling upstream of NF-kappaB activation, such as degradation of inhibitor factor-kappaBalpha (IkappaBalpha ) and phosphorylations of extracellular signal... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057343 Sat, 05 Dec 2009 03:58:54 +0100 3057343 http://www.medworm.com/index.php?rid=3057342&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956902%26dopt%3DAbstract Authors: Lui Z, Ma Q, Wang X, Zhang Y Many reports show that vascular endothelial growth factor receptor 3 (VEGFR3) plays an essential role in tumor metastasis and is a promising target for cancer therapy. The present study was designed to determine the role of VEGFR3 in tumor growth using RNA interference (RNAi) technology. Three small interfering RNA (siRNA) sequences for the VEGFR3 gene were cloned into expression plasmids (pSUPER) and transfected into human colorectal carcinoma (CRC) LoVo cells. Stable transfection of these plasmids decreased VEGFR3 protein expression, leading to the potent suppression of tumor cell proliferation and lymphangiogenesis in vitro. Furthermore, we selected the most effective silenced expressor vector and injected it and pSUPER vector into a tumor xenog... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057342 Sat, 05 Dec 2009 03:58:52 +0100 3057342 http://www.medworm.com/index.php?rid=3057341&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956903%26dopt%3DAbstract In this study, cultures of fusigenic (BeWo) and non-fusigenic (JEG-3) human choriocarcinoma cell lines were used to investigate the expression of mTOR and its downstream signalling components. The effects of an inducer of syncytialisation (forskolin) on mTOR, eIF4E binding proteins (4EBPs) and ribosomal protein S6 kinases (S6Ks) in BeWo cells were also assessed. RT-PCR studies revealed that mTOR, 4EBP and S6Ks are expressed at mRNA level in both JEG-3 and BeWo cells. Semi-quantitative RT-PCR analysis revealed that in early stages of syncytialisation (50 microM forskolin for 48 h), the expression of mTOR and 4EBP was down-regulated when compared to unstimulated cells. In fully syncytialised cells (50 microM forskolin for 72 h) the expression of both genes was similar to basal levels. Intere... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057341 Sat, 05 Dec 2009 03:58:49 +0100 3057341 http://www.medworm.com/index.php?rid=3057340&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956904%26dopt%3DAbstract Authors: Gao CL, Zhu JG, Zhao YP, Chen XH, Ji CB, Zhang CM, Zhu C, Xia ZK, Peng YZ, Guo XR Uncoupling proteins (UCPs) belong to a superfamily of mitochondrial transporters that uncouple ATP synthesis from electron transport. We have previously shown that uncoupling protein 4 (UCP4) is differentially expressed in omental adipose tissue in diet-induced obese and normal rats. Overexpression of UCP4 promotes proliferation and inhibits apoptosis and differentiation of preadipocytes. In this work, we further characterized the effect of UCP4 on mitochondrial function in mature 3T3-L1 adipocytes. Transmission electron microscopy (TEM) showed that adipocytes overexpressing UCP4 displayed condensed mitochondria with twisted, condensed, and unclear cristae. Moreover, the loss of the mitochondrial... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057340 Sat, 05 Dec 2009 03:58:46 +0100 3057340 http://www.medworm.com/index.php?rid=3057339&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956905%26dopt%3DAbstract Authors: Hayashi S, Sakurai H, Hayashi A, Tanaka Y, Hatashita M, Shioura H We investigated the mechanisms of thermosensitization related to combination therapy with sesquiterpene lactone parthenolide (PTL), a nuclear factor-kappaB (NF-kappaB) inhibitor, and hyperthermia using human lung adenocarcinoma cells A549. The kinetics of apoptosis induction and cell cycle of cells treated with PTL, heating, and combined treatment were examined by flow cytometric analysis. The flow cytometric distribution was calculated and expressed as a percentage. The ratios of the sub-G1 division, used to determine the induction of apoptosis, increased significantly with the combination therapy. Furthermore, the ratios of G2/M division increased and the ratios of G0/G1 division decreased, indicating cell cyc... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057339 Sat, 05 Dec 2009 03:58:43 +0100 3057339 http://www.medworm.com/index.php?rid=3057338&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956906%26dopt%3DAbstract Authors: Bartling B, Koch A, Simm A, Scheubel R, Silber RE, Santos AN The insulin-like growth factor (IGF) system is involved in cell migration, which plays an important role in cancer progression. It has been shown that cancer progression correlates with the level of circulating human hematopoietic stem and progenitor cells (HSPCs) expressing CD34 and/or CD133. However, it is unknown whether factors released from cancer cells, including soluble compounds of the IGF system, recruit these HSPCs via enhancing their migration. Our study showed the expression of type I IGF receptor (IGF-IR) in human HSPCs expressing CD34 and/or CD133. In an indirect co-culture model, soluble factors released from human lung epithelial cancer cells (H358, H322) increased the migration of CD34-/CD133+ cells ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057338 Sat, 05 Dec 2009 03:58:40 +0100 3057338 http://www.medworm.com/index.php?rid=3057337&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956907%26dopt%3DAbstract Authors: Takai N, Ueda T, Nishida M, Nasu K, Narahara H The potential anticancer agent 1-(2-chlorophenyl-N-methylpropyl)-3-isoquinolinecarboxamide (PK11195), a translocator protein ligand, facilitates the induction of cell death by a variety of cytotoxic and chemotherapeutic agents in various human tumor cell lines. The purpose of this study was to elucidate the effect of PK11195 on three endometrial cancer cell lines, two ovarian cancer cell lines and normal human endometrial epithelial cells. Endometrial and ovarian cancer cells were treated with various concentrations of PK11195 alone or in combination with chemotherapeutic drugs (cisplatin, paclitaxel), and its effect on cell growth, the cell cycle, apoptosis and related measurements was investigated. The 3-(4,5-dimethylthiazol-2-y... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057337 Sat, 05 Dec 2009 03:58:37 +0100 3057337 http://www.medworm.com/index.php?rid=3057336&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956908%26dopt%3DAbstract Authors: Takai S, Tokuda H, Matsushima-Nishiwaki R, Saio M, Takami T, Kozawa O Ag-specific effector/memory CD8+ T cells play an important role, not only in viral eradication, but also in T cell-mediated tumor rejection. Increasing evidence suggests that TGF-beta plays a critical role in the tumor escape from immune surveillance. Although it is known that TGF-beta directly suppresses the activation of naïve T cells, the direct effects of TGF-beta on effector/memory CD8+ T cells have not yet been fully investigated. The present study evaluated the effect of TGF-beta on effector/memory CD8+ T cells using Ag-specific, mouse-derived, effector/memory CD8+ T cell clones, designated as 6C2. Notably, pretreatment of TGF-beta1 caused an approximate 100% enhancement of IFN-gamma production i... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057336 Sat, 05 Dec 2009 03:58:34 +0100 3057336 http://www.medworm.com/index.php?rid=3057335&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956909%26dopt%3DAbstract Authors: Doh KO, Lee YH, Han KH, Uhm SY, Kim JP, Bae YU, Park JH, Moon IJ, Park JG In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 c... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057335 Sat, 05 Dec 2009 03:58:30 +0100 3057335 http://www.medworm.com/index.php?rid=3057334&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956910%26dopt%3DAbstract In conclusion, ATO differentially affected cell growth inhibition and death depending on the incubation dose and cell type. The changes in ROS and GSH levels by ATO were not tightly correlated with the level of cell death. Our present data provide useful information for the action of ATO in various cell types in relation to cell growth, cell death, ROS and GSH levels. PMID: 19956910 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057334 Sat, 05 Dec 2009 03:58:28 +0100 3057334 http://www.medworm.com/index.php?rid=3057333&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956911%26dopt%3DAbstract Authors: Park KH, Shin DG, Kim JR, Hong JH, Cho KH To compare the molecular composition and functional differences at the lipoprotein level, we analyzed individual lipoprotein fractions from male patients with metabolic syndrome (MetS) (n=10) and gender- and age-matched healthy controls (n=14). The MetS group had significantly higher obesity, blood pressure, serum cholesterol, triglyceride (TG), adiponectin, and uric acid levels than the control group, while the serum blood urea nitrogen and creatinine levels of the MetS group were in the normal range. The MetS group had much weaker serum antioxidant ability and were more susceptible to copper-mediated low-density lipoprotein (LDL)-oxidation. TG and apoC-III co-accumulated with LDL, high density lipoprotein (HDL)2, and HDL3 in the MetS... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057333 Sat, 05 Dec 2009 03:58:25 +0100 3057333 http://www.medworm.com/index.php?rid=3057332&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956912%26dopt%3DAbstract In conclusion, transgenic mice expressing SCCA1 showed higher liver regenerative potential compared to wild-type mice, supporting the dual role of this serpin as an anti-apoptotic and pro-proliferative stimulus for liver cells in vivo. PMID: 19956912 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057332 Sat, 05 Dec 2009 03:58:22 +0100 3057332 http://www.medworm.com/index.php?rid=3057331&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956913%26dopt%3DAbstract Authors: Molinari C, Iorio P, Medri L, Ballardini M, Guiducci G, Cremonini AM, Cerasoli S, Riccioni L, Faedi M, Mariani GA, Zoli W, Silvestrini R, Calistri D Oligodendrogliomas are rare primary brain tumors with variable patient outcomes which are not always adequately accounted for by clinical or pathological variables. The present study evaluated the prognostic implications of chromosome 1p and 19q status in a set of 23 low grade oligodendrogliomas (OGD II), and correlated the results with patient outcome. Loss of heterozygosity (LOH) and fluorescent in situ hybridization (FISH) analyses, the most widely used standard procedures, were used. 1p and 19q deletions were found in 65 and 61% of cases, respectively, using FISH and in 78 and 72% of cases using LOH. Both deletions were found ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057331 Sat, 05 Dec 2009 03:58:18 +0100 3057331 http://www.medworm.com/index.php?rid=3057330&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956914%26dopt%3DAbstract Authors: Yano T, Tanaka M, Fukuda N, Ueda T, Nagase H Mutant mitochondrial (mt) DNA variants are related to human disease and have been investigated using cytoplasmic hybrid (cybrid) cells generated from human tumor cells in which mutant mt maintenance depends on the cell line. It is, however, unclear whether human intercellular fusion of non-tumorous cells influences the maintenance of disease-related mutant mt. A preliminary experiment of cell-cell fusion between a human skin fibroblast cell line from a Lesch-Nyhan syndrome patient and an osteosarcoma cybrid cell line harboring the mitochondrial tRNALeu(UUR)A3243G mutation showed a decrease of A3243G mutant mtDNA in fused cells during passages. In order to confirm the decrease of mutant mtDNA, we performed cell-cell fusion experiment... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057330 Sat, 05 Dec 2009 03:58:15 +0100 3057330 http://www.medworm.com/index.php?rid=3057329&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956915%26dopt%3DAbstract Authors: Jacob A, Rajan D, Pathickal B, Balouch I, Hartman A, Wu R, Zhou M, Wang P Hepatocellular dysfunction occurs early in sepsis and this appears to be caused by Kupffer cell-derived TNF-alpha production from the liver as a result of the increased release of the sympathetic neurotransmitter, norepinephrine, from the gut. Ghrelin, a novel stomach-derived peptide, is down-regulated in sepsis and administration of ghrelin into rodents decrease pro-inflammatory cytokines, attenuates hepatic and other organ injuries and improves survival. Ghrelin's beneficial effect in sepsis is mediated by the inhibition of the sympathetic nervous system (SNS), as evidenced by the reduced gut-derived norepineprine (NE) release in sepsis after ghrelin treatment. Recent data suggest that MKP-1, the MAPK ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057329 Sat, 05 Dec 2009 03:58:12 +0100 3057329 http://www.medworm.com/index.php?rid=3057328&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956916%26dopt%3DAbstract Authors: Shih CM, Chen CY, Lee IH, Kao WT, Wang YC Polymorphisms in DNA repair genes may be associated with differences in repair capacity of DNA damage and may thereby influence an individual's susceptibility to lung cancer. We investigated the association between the -93Gright curved arrow A polymorphism in the mismatch repair hMLH1 gene for its role in the susceptibility and survival of non-small cell lung cancer (NSCLC) patients. Using a case-control study design, 165 NSCLC patients and 193 controls with similar range for age, gender and smoking habit distributions were subjected to genotype analysis. The risk of lung cancer was estimated by logistic regression analysis. The Kaplan-Meier method was used to estimate the probability of survival and the log-rank test was used to asses... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057328 Sat, 05 Dec 2009 03:58:09 +0100 3057328 http://www.medworm.com/index.php?rid=3057327&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19956917%26dopt%3DAbstract Authors: Carpentier YA, Hacquebard M, Portois L, Malaisse WJ Exposure of normal rats for 3-7 months to an omega3-deprived diet and subsequent exposure to an omega3-enriched diet were recently proposed as a model to study the metabolic consequences of alteration in the dietary supply of omega3 PUFA and their time course. The same animal model was used in the present study, which aimed at characterizing the pattern of saturated and monodesaturated fatty acids in the phospholipids of the duodenum, jejunum, caecum and colon. With one exception (C18:0), the weight content of these fatty acids was lower in the proximal than distal intestinal segments, a situation possibly accounted for by the generation of short-chain fatty acids by the colonic flora and the resulting synthesis of longer fat... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=3057327 Sat, 05 Dec 2009 03:58:05 +0100 3057327 http://www.medworm.com/index.php?rid=2960148&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885611%26dopt%3DAbstract Authors: El-Salhy M Ghrelin is a peptide hormone, which has been isolated from the stomach. It is localized mostly in endocrine cells in the oxyntic mucosa of the stomach. Ghrelin receptors are expressed equally in all parts of the gastrointestinal tract, with a similar level of expression in the mucosal and muscle layers. This peptide hormone has several functions, the most widely known is its growth hormone (GH)-releasing effect. Ghrelin plays an important role in regulating appetite, feeding and energy metabolism. It also plays a role in mediating immune response and inflammatory processes. Ghrelin stimulates gastric motility and emptying as well as motility in the small and large intestine. Ghrelin has been reported to be affected in several gastrointestinal diseases/disorders such... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960148 Thu, 05 Nov 2009 12:43:01 +0100 2960148 http://www.medworm.com/index.php?rid=2960147&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885612%26dopt%3DAbstract Authors: Osawa N, Onoda N, Kawajiri H, Tezuka K, Takashima T, Ishikawa T, Miyauchi A, Hirokawa M, Wakasa K, Hirakawa K The differential diagnosis of parathyroid carcinoma from benign adenoma is often difficult when its typical clinicopathological features are absent, even with the aid of various molecular markers. We recently demonstrated that telomerase activation through hTERT expression is a unique characteristic that is limited to parathyroid carcinoma and not seen in benign tumors. In the present study, we investigated hTERT expression in parathyroid tumors using immunohistochemistry in an attempt to determine its clinical utility. There was no evidence of immunoreactivity in the 4 normal parathyroid glands and the 18 typical adenomas. In contrast, one atypical adenoma stained pos... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960147 Thu, 05 Nov 2009 12:42:58 +0100 2960147 http://www.medworm.com/index.php?rid=2960146&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885613%26dopt%3DAbstract Authors: Proia P, Schiera G, Salemi G, Ragonese P, Savettieri G, Di Liegro I An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secondary to myelin damage, as generally assumed. SPMS serum affects the permeability of the BBB m... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960146 Thu, 05 Nov 2009 12:42:56 +0100 2960146 http://www.medworm.com/index.php?rid=2960145&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885614%26dopt%3DAbstract We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state analogue TOCSY (TOtal Correlation SpectroscopY). Using 1- and 2-dimensional HRMAS NMR experiments, we identified several metabolites in burned tissues. Quantification of me... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960145 Thu, 05 Nov 2009 12:42:53 +0100 2960145 http://www.medworm.com/index.php?rid=2960144&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885615%26dopt%3DAbstract Authors: Aleem AM, Wells L, Jankun J, Walther M, Kühn H, Reinartz J, Skrzypczak-Jankun E The single nucleotide polymorphism (SNP) R261Q in the human platelet 12-lipoxygenase has been correlated with several human diseases. To understand better the biological performance we have compared enzymatic properties of the recombinant enzymes: 'wild-type' as Q261 and R261 variants with a single Q261R mutation at the enzyme periphery and N544L mutant with an altered active site. The R261 variant does not follow the same kinetics such as WT-Q261 showing a lag phase, a slower accumulation of product, following a different time-course without reaching plateau characteristic for the Q261 variant. The N544L substitution in the active site almost eradicates enzymatic activity proving that asparag... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960144 Thu, 05 Nov 2009 12:42:51 +0100 2960144 http://www.medworm.com/index.php?rid=2960143&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885616%26dopt%3DAbstract Authors: Ji NY, Park MY, Kang YH, Lee CI, Kim DG, Yeom YI, Jang YJ, Myung PK, Kim JW, Lee HG, Kim JW, Lee K, Song EY Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca27+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR and Western blot analysis. For quantitative analysis... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960143 Thu, 05 Nov 2009 12:42:48 +0100 2960143 http://www.medworm.com/index.php?rid=2960142&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885617%26dopt%3DAbstract This study was performed to determine the anti-cancer activity of tanshinone IIA on human breast cancer cells in vitro and in vivo and to elucidate the underlying mechanism of this activity. Human breast cancer cell lines (estrogen receptor-positive and -negative) were treated with tanshinone IIA and tamoxifen. The inhibitory effects of tanshinone IIA and tamoxifen on breast cancer cell proliferation were examined using MTT assays, BrdU incorporation, immunohistochemistry and flow cytometry. Upon treatment with tanshinone IIA, breast cancer cell proliferation was significantly inhibited in a dose- and time-dependent manner (IC50 = 0.25 microg/ml) and apoptotic cell populations increased, while tamoxifen inhibited only ER-positive breast cancer cells prominently and had no effect on ER-nega... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960142 Thu, 05 Nov 2009 12:42:45 +0100 2960142 http://www.medworm.com/index.php?rid=2960141&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885618%26dopt%3DAbstract Authors: Ziolkowska A, Rucinski M, Tyczewska M, Malendowicz LK Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar to NPBWR2 genes in cultured rat calvarial osteoblast-like (ROB) cells and the effects of both peptides on pr... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960141 Thu, 05 Nov 2009 12:42:42 +0100 2960141 http://www.medworm.com/index.php?rid=2960140&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885619%26dopt%3DAbstract Authors: Chen J, Watanabe M, Huang P, Sakaguchi M, Ochiai K, Nasu Y, Ouchida M, Huh NH, Shimizu K, Kashiwakura Y, Kaku H, Kumon H The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones in comparison to the control. The apopto... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960140 Thu, 05 Nov 2009 12:42:40 +0100 2960140 http://www.medworm.com/index.php?rid=2960139&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885620%26dopt%3DAbstract In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly increased, and CaMKIIalpha expression significantly decreased (P<0.05) in the basolateral amygdala after SPS. These findings suggest dysfunction of Ca2+-CaM-CaMKIIalpha ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960139 Thu, 05 Nov 2009 12:42:36 +0100 2960139 http://www.medworm.com/index.php?rid=2960138&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885621%26dopt%3DAbstract Authors: Chen HM, Yan XJ, Mai TY, Wang F, Xu WF Previous studies have shown that highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) possess an anti-angiogenetic effect, while high concentrations of lambda-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of lambda-CO on inhibiting cell proliferation. lambda-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, prevention of oxidative stress by N-acetyl-L-cysteine (NAC) could abolish the effect of lambda-CO on these events. Yet, lambd... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960138 Thu, 05 Nov 2009 12:42:34 +0100 2960138 http://www.medworm.com/index.php?rid=2960137&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885622%26dopt%3DAbstract In this study, we established 293T cells stably expressing human TrkB to elucidate its intracellular functions. Using this cell system, we examined the biological roles of TrkB and its downstream target molecules. The TrkB expressing cells showed an increased survival rate through increased c-fos mRNA expression by BDNF, which were completely suppressed by TrkB inhibitor. Moreover, the combination of inhibitors of mitogen-activated protein kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) partially reduced both the cell survival rate and c-fos mRNA expression, whereas monotreatment of these reagents could not affect cell survival nor c-fos mRNA expression. These results suggested that TrkB could play a role in c-fos-associated cell survival through both MEK and PI3K pathway. It is conc... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960137 Thu, 05 Nov 2009 12:42:31 +0100 2960137 http://www.medworm.com/index.php?rid=2960136&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885623%26dopt%3DAbstract Authors: Bozzini S, Gambelli P, Boiocchi C, Schirinzi S, Falcone R, Buzzi P, Storti C, Falcone C Cardiovascular disease (CVD) and depression are two of the most common human health problems. Patients with depression have an increased risk of developing cardiovascular disease and mortality after experiencing a cardiac event. Both diseases are complex disorders that are influenced by genetic and environmental factors. Brain-derived neuro-trophic factor (BDNF) plays a critical role in regulating both vascular development and response to injury, and promotes survival, differentiation, and maintenance of neurons in the peripheral and nervous system. Evidence suggests that BDNF can enhance serotoninergic transmission. Serotonin modulates different brain functions and is known to regulate sle... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960136 Thu, 05 Nov 2009 12:42:29 +0100 2960136 http://www.medworm.com/index.php?rid=2960135&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885624%26dopt%3DAbstract In this study, we isolated a novel glycoprotein from L. japonica that stimulates the growth of the IEC-6 normal murine intestinal epithelial cells. We also identified the mechanism by which this glycoprotein, referred to as LJGP, stimulates cell growth. After 24 h of exposure to LJGP, cell proliferation increased in a dose-dependent manner. To further explore the mechanism associated with LJGP-induced cell proliferation, we treated cells for various times with LJGP. We focused on the epidermal growth factor receptor (EGFR) signaling pathway, which is involved in the regulation of cellular proliferation and differentiation, during LJGP-induced cell growth. The results showed that LJGP induced EGFR and Akt activation. Furthermore, LJGP stimulated Shc/Grb2 binding and ERK activation, but inhi... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960135 Thu, 05 Nov 2009 12:42:26 +0100 2960135 http://www.medworm.com/index.php?rid=2960134&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885625%26dopt%3DAbstract In this study, we evaluated the expression of lipid metabolism-associated genes in patients with HCV infection by real-time PCR. Sterol regulatory element-binding protein (SREBP)-2 expression was unchanged and low density lipoprotein receptor expression was markedly reduced by 90% in HCV-infected liver. The expression of apolipoprotein B100, microsomal triglyceride transfer protein and ATP-binding cassette G5 was significantly increased. Up-regulation of cholesterol synthesis-associated genes, including HMG-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase and squalene synthase, confirmed enhanced de novo cholesterol synthesis. The expression of cholesterol 7alpha-hydroxylase and farnesoid X receptor was enhanced, while bile salt export pump expression was unchanged. Fatty aci... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960134 Thu, 05 Nov 2009 12:42:24 +0100 2960134 http://www.medworm.com/index.php?rid=2960133&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885626%26dopt%3DAbstract Authors: Ohira M, Oshitani N, Hosomi S, Watanabe K, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Crohn's disease is associated with increased permeability of the intestine even in quiescent patients. Increased intestinal permeability may cause dysregulated immunological responses in the intestinal mucosa that leads to chronic intestinal inflammation. Tight junction proteins contribute to intestinal permeability, and functional abnormality and dislocation of such proteins may cause increased intestinal permeability. We studied the expression of tight junction proteins Rab13, vasodilator-stimulated phosphoprotein (VASP), zonula occludin-1 (ZO-1), and F-actin in the intestinal epithelium of patients with inactive inflammatory bowel disease. Surgical sampl... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960133 Thu, 05 Nov 2009 12:42:20 +0100 2960133 http://www.medworm.com/index.php?rid=2960132&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885627%26dopt%3DAbstract Authors: De Angeli S, Baiguera S, Del Pup L, Pavan E, Gajo GB, Di Liddo R, Conconi MT, Grandi C, Schiavon O, Parnigotto PP Cord blood (CB) is a source of hematopoietic stem cells (HSCs) and is an alternative to bone marrow for allogenic transplantation in patients with hematological disorders. The improvement of HSC in vitro expansion is one of the main challenges in cell therapy. Stromal components and soluble factors, such as cytokines, can be useful to induce in vitro cell expansion. Hence, we investigated whether feeder-layers from new stromal cell lines and different exogenous cytokine cocktails induce HSC expansion in middle-term cultures. CB HSC middle-term expansion was carried out in co-cultures on different feeder-layers exposed to three different cytokine cocktails. CB HSC e... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960132 Thu, 05 Nov 2009 12:42:17 +0100 2960132 http://www.medworm.com/index.php?rid=2960131&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885628%26dopt%3DAbstract Authors: Zoico E, Garbin U, Olioso D, Mazzali G, Fratta Pasini AM, Di Francesco V, Sepe A, Cominacini L, Zamboni M It was recently suggested that the transcription nuclear factor-kappaB (NF-kappaB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-kappaB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 microg/ml lipopolysaccharides (LPS) for 16 h, with or without pre-treatment with 10 microg/ml of glo... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960131 Thu, 05 Nov 2009 12:42:15 +0100 2960131 http://www.medworm.com/index.php?rid=2960130&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885629%26dopt%3DAbstract Authors: Kim YS, An HT, Kim J, Ko J Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in a dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin, a specific inhibitor of protein kinase Cdelta (PKCdelta). In addition, taxol-induced MCP-1 expression was reduced by the ectopic exp... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960130 Thu, 05 Nov 2009 12:42:12 +0100 2960130 http://www.medworm.com/index.php?rid=2960129&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885630%26dopt%3DAbstract Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA A dietary deprivation in long-chain polyunsaturated omega3 fatty acids initiated in 7-week old normal rats provokes within 3 to 7 months the appearance of several features of the metabolic syndrome. Likewise, within 2 to 4-5 weeks exposure to a flaxseed oil-enriched diet, these anomalies are rapidly corrected. The present study deals with the omega3 fatty acid content of intestinal phospholipids under the same experimental conditions. For the sake of comparison, the control rats were given access during the last 4-5 weeks to either a soybean or flaxseed oil-enriched diet. In control rats, the relative weight content of omega3 fatty acids as well as their product/precursor ratio differed in distinct segments of the intestinal ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960129 Thu, 05 Nov 2009 12:42:10 +0100 2960129 http://www.medworm.com/index.php?rid=2960128&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885631%26dopt%3DAbstract This study aims mainly at investigating the effects of a dietary deprivation and replenishment of omega3 PUFA upon the phospholipid pattern of omega6 PUFA in the duodenum, jejunum, caecum and colon of rats exposed for 3-7 months to an omega3-depleted diet and then eventually exposed for 2-4 weeks to an omega3-rich diet. In control rats, the relative weight content of all omega6 fatty acids differed in the proximal and distal intestinal segments. In the omega3-depleted rats the C18:2omega6, C20:2omega6 and C20:3omega6 content was decreased whilst that of C20:4omega6 and C22:4omega6 was increased. Significant correlations were found in the caecum or colon between the C18:2omega6 or C20:4omega6 content of intestinal phospholipids and their C22:6omega3 content, an increase in the latter conten... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2960128 Thu, 05 Nov 2009 12:42:07 +0100 2960128 http://www.medworm.com/index.php?rid=2847334&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787192%26dopt%3DAbstract Authors: Ye L, Bokobza SM, Jiang WG Bone morphogenetic proteins (BMPs) belong to the TGF-beta superfamily, which plays important roles in foetal and postnatal development and also maintains the homeostasis of various tissues and organs. Due to the critical role played by BMPs in bone formation and bone turnover, the implication of these molecules in bone metastasis has been intensively studied over the past decade. BMPs have been implicated in the development and progression of solid tumours, particularly the disease-specific bone metastasis. In breast cancer, a tumour type which most commonly metastasizes to bones, aberrations of both BMP expression and their signalling have been recently demonstrated. These aberrations have certain correlations with the development and progression of... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847334 Thu, 01 Oct 2009 06:18:44 +0100 2847334 http://www.medworm.com/index.php?rid=2847333&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787193%26dopt%3DAbstract In this study, we report that STEAP4 expression is able to inhibit anchorage-independent cell growth. We also demonstrate that STEAP4 associates with focal adhesion kinase (FAK) and regulate the activity of FAK through Y397 phosphorylation. Furthermore, we show that CpG sequences in STEAP4 promoter region were frequently methylated in DU145, androgen-independent prostate cancer cells. Demethylation treatment induced STEAP4 expression in DU145, suggesting the possibility that STEAP4 expression in cancer cells is in part epigenetically regulated. Collectively, these data demonstrate a novel function of STEAP4 and that STEAP4 may play an important role in tumor malignancy. PMID: 19787193 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847333 Thu, 01 Oct 2009 06:18:42 +0100 2847333 http://www.medworm.com/index.php?rid=2847332&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787194%26dopt%3DAbstract In this study, we performed immunoscreening using the serum from a patient with Isaac's syndrome and identified the novel gene named Kenae/CCDC125. Expression analysis of Kenae/CCDC125 revealed that its transcript was highly expressed in tissues associated with the immune system, such as the thymus, spleen and bone marrow. In cells stably expressing Kenae/CCDC125, delay in cell motility and deregulation of RhoGTPase (RhoA, Rac1 and cdc42) activity to extracellular stimuli were demonstrated. These results suggest that the novel gene, Kenae/CCDC125, acts as a regulator of cell motility through RhoA, Rac1 and cdc42. PMID: 19787194 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847332 Thu, 01 Oct 2009 06:18:39 +0100 2847332 http://www.medworm.com/index.php?rid=2847331&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787195%26dopt%3DAbstract Authors: Stathopoulos GP, Armakolas A Cytogenetic and molecular studies have identified imbalanced chromosomal regions leading to the characterization of several candidate genes. Differences in gene expression were examined in the blood by whole genome microarray analysis among individuals with double or single primary malignancies and healthy individuals. Twenty-four individuals with at least two primary malignancies of the breast and/or colon and/or ovary were compared with 32 individuals with single breast, colon or ovarian cancer. The single malignancy group had a median duration of disease of 9 years (range 5-23 years). Validation was obtained by examining each patient separately with quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) analysis for the ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847331 Thu, 01 Oct 2009 06:18:37 +0100 2847331 http://www.medworm.com/index.php?rid=2847330&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787196%26dopt%3DAbstract Authors: Liu JJ, Guo YW, Fang ZG, Si XN, Wu XY, Liu PQ, Lin DJ, Xiao RZ, Xu Y, Wang CZ, Li XD, He Y, Huang RW In the present study we investigated the in vitro apoptosis inducing effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand ciglitazone (CGZ) on acute promyelocytic leukemia (APL) NB4 cells and its mechanisms of action. The results revealed that CGZ (10-50 micromol/l) inhibited the growth of leukemia NB4 cells and caused apoptosis in a time- and dose-dependent manner. Apoptosis was observed clearly by flow cytometry (FCM) and DNA fragmentation analysis. After treatment by CGZ for 48 h, the percentage of disruption of mitochondrial membrane potential (Deltapsim) was increased in a dose-dependent manner. Western blotting demonstrated the cleavage of caspa... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847330 Thu, 01 Oct 2009 06:18:34 +0100 2847330 http://www.medworm.com/index.php?rid=2847329&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787197%26dopt%3DAbstract Authors: Perchellet JP, Perchellet EM, Crow KR, Buszek KR, Brown N, Ellappan S, Gao G, Luo D, Minatoya M, Lushington GH Pilot-scale libraries of eight-membered medium ring lactams (MRLs) and related tricyclic compounds (either seven-membered lactams, thiolactams or amines) were screened for their ability to inhibit the catalytic activity of human recombinant 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase in vitro. A dozen of the synthetic compounds mimic the inhibition of purified HMG-CoA reductase activity caused by pravastatin, fluvastatin and sodium salts of lovastatin, mevastatin and simvastatin in this cell-free assay, suggesting direct interaction with the rate-limiting enzyme of cholesterol biosynthesis. Moreover, several MRLs inhibit the metabolic activity of L1210 t... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847329 Thu, 01 Oct 2009 06:18:32 +0100 2847329 http://www.medworm.com/index.php?rid=2847328&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787198%26dopt%3DAbstract Authors: Herouy Y, Kreis S, Mueller T, Duerk T, Martiny-Baron G, Reusch P, May F, Idzko M, Norgauer Y Lipodermatosclerosis refers to skin induration of the lower extremities characterized by tortuous, hyperpermeable vessels preceding venous leg ulcerations. Protein ligands and receptor tyrosine kinases that specifically regulate endothelial cell function are mainly involved in physiological as well as in disease-related angiogenesis. These ligand/receptor systems include the vascular endothelial growth factor (VEGF) and the angiopoietin (Ang) families and their receptor the tyrosine kinase with immunoglobulin-like domains (Tie-2) as well as the VEGF receptor family (VEGF-R1 and VEGF-R2). In the present study, the contribution of these endothelium-specific ligand/receptor systems in tis... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847328 Thu, 01 Oct 2009 06:18:26 +0100 2847328 http://www.medworm.com/index.php?rid=2847327&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787199%26dopt%3DAbstract In conclusion, peptide 19 induced HepG2 cell apoptosis through the mitochondrial apoptosis pathway. PMID: 19787199 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847327 Thu, 01 Oct 2009 06:18:24 +0100 2847327 http://www.medworm.com/index.php?rid=2847326&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787200%26dopt%3DAbstract In this study, we investigated the cell density-dependent induction of apoptosis of human acute myeloid leukemia U937 and ML-1 cells by disulfiram (DSF), the dithiocarbamate drug recently proposed for treatment of human cancers. This effect is dependent on uptake of extracellular copper and its intracellular accumulation. High-density cells cannot uptake and accumulate this metal to a sufficient level that would allow induction of apoptosis due to progressive decrease of its extracellular concentration. Simple addition of copper can resume sensitivity of high-density leukemic cells to DSF and improve efficiency of anti-leukemic therapies using this drug, thus providing benefit to patients with high WBC count. PMID: 19787200 [PubMed - in process] (Source: International Journal of Molecu... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847326 Thu, 01 Oct 2009 06:18:22 +0100 2847326 http://www.medworm.com/index.php?rid=2847325&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787201%26dopt%3DAbstract This study suggests that the carotenoid beta-cryptoxanthin may promote osteoblast differentiation and activity by amplifying TGF-beta1-induced lineage commitment of osteoblast precursors. PMID: 19787201 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847325 Thu, 01 Oct 2009 06:18:19 +0100 2847325 http://www.medworm.com/index.php?rid=2847324&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787202%26dopt%3DAbstract Authors: Kopp R, Fichter M, Schalhorn G, Danescu J, Classen S CD44 is a transmembrane glycoprotein involved in the interaction between cells and the extracellular matrix. A large variety of alternatively spliced CD44 variants are expressed by different tumors with possible implication for tumor progression, formation of metastasis and survival. In colon carcinomas, previous reports described higher molecular bands of CD44 transcripts in neoplastic colonic tissue, although a complete analysis of multiple combinations of CD44v transcripts were not performed. We therefore analyzed the pattern of CD44 standard and variant (v2-v10) transcripts in colorectal adenomas and carcinomas by exon-specific RT-PCR amplification and sought CD44v transcripts specific for colonic neoplasias. Our data in... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847324 Thu, 01 Oct 2009 06:18:17 +0100 2847324 http://www.medworm.com/index.php?rid=2847323&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787203%26dopt%3DAbstract Authors: Cong M, Liu T, Wang P, Xu Y, Tang S, Wang B, Jia J, Liu Y, Hermonat PL, You H Elevated tissue inhibitor of metalloproteinase (TIMP)-1 expression contributes to excess production of extracellular matrix in liver fibrosis. However, there are few studies on sustained suppression of TIMP-1. We aimed to construct a recombinant adeno-associated virus (AAV) carrying small interfering RNAs (siRNAs) of TIMP-1 and investigate the long-term effects of RNA interference upon the TIMP-1 gene in rat hepatic stellate cells (HSCs). Five siRNA oligomers targeting rat TIMP-1 were designed and transfected into HSCs. A U6 promoter followed by the siRNA which had the strongest suppression effect was cloned into the AAV vector and packed into 293 cells to construct the recombinant AAV/siRNA-TIMP-1/n... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847323 Thu, 01 Oct 2009 06:18:14 +0100 2847323 http://www.medworm.com/index.php?rid=2847322&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787204%26dopt%3DAbstract Authors: Monari M, Foschi J, Calabrese C, Liguori G, Di Febo G, Rizzello F, Gionchetti P, Trinchero A, Serrazanetti GP The present study is the first to evaluate the expression and activity of MnSOD, Cu/ZnSOD and catalase in human gastric samples, since ROS play a significant role in the pathogenesis of different forms of malignancy inducing mutations and various diseases such as gastric cancer. Biopsies and surgical samples from 53 patients (male/female 22/31, mean age 56.5+/-15.8 years) consisted of 15 healthy, 12 autoimmune atrophic gastritis, 10 Helicobacter pylori (HP) infection, 8 HP-negative chronic gastritis (CG) and 8 adenocarcinoma cases. Enzyme activity and expression were evaluated by spectrophotometry and immunoblotting after specific extraction in phosphate buffer. We fou... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847322 Thu, 01 Oct 2009 06:18:12 +0100 2847322 http://www.medworm.com/index.php?rid=2847321&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787205%26dopt%3DAbstract Authors: Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y Hypertension and diabetes mellitus are important risk factors for myocardial infarction (MI). The purpose of the present study was to identify genetic variants that confer susceptibility to MI in individuals with or without hypertension or diabetes mellitus, thereby contributing to the personalized prevention of MI in such individuals. The study population comprised 5,835 unrelated Japanese individuals, including 1,339 subjects with MI and 4,496 controls. The 150 polymorphisms were selected by genome-wide association studies of MI and ischemic stroke with the use of the GeneChip Human Mapping 500K Array Set. The genotypes for these polymorphisms were determined by a meth... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847321 Thu, 01 Oct 2009 06:18:09 +0100 2847321 http://www.medworm.com/index.php?rid=2847320&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787206%26dopt%3DAbstract Authors: Shipley PR, Donnelly CC, Le CH, Bernauer AD, Klegeris A Asukamycin, a manumycin-type metabolite, was isolated by a rapid and easily scalable purification scheme. Thus far, studies on the biological activity of asukamycin have been limited to its role as an antibacterial and antifungal agent. By using five different tumor cell lines we demonstrate antineoplastic activity of asukamycin. It inhibited cell growth at concentrations similar to other members of the manumycin family (IC50 1-5 microM). Cytotoxicity of asukamycin was accompanied by activation of caspases 8 and 3 and was diminished by SB 202190, a specific p38 mitogen-activated protein kinase (MAPK) inhibitor. These data, in combination with earlier observations showing its low in vivo toxicity, indicate that further stu... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847320 Thu, 01 Oct 2009 06:18:07 +0100 2847320 http://www.medworm.com/index.php?rid=2847319&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19787207%26dopt%3DAbstract In conclusion, PG induced apoptosis via the loss of MMP (DeltaPsi(m)) in CPAEC, which is accompanied by GSH depletion. JNK and p38 inhibitors increased and decreased apoptosis in PG-treated CPAEC, respectively, which were correlated with GSH depletion. PMID: 19787207 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2847319 Thu, 01 Oct 2009 06:18:04 +0100 2847319 http://www.medworm.com/index.php?rid=2759890&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724879%26dopt%3DAbstract This study discusses the development of cosmetic textiles and addresses microencapsulation technology with respect to its historical background, significant advantages, microencapsulation methods and recent applications in the textile industry. Gelatin microcapsules containing vitamin C were prepared using emulsion hardening technique. Both the optical microscopy and scanning electron microscopy demonstrated that the newly developed microcapsules were in the form of core-shell spheres with relatively smooth surface. The particle size of microcapsules ranged from 5.0 to 44.1 microm with the average particle size being 24.6 microm. The gelatin microcapsules were proved to be non-cytotoxic based on the research findings of the toxicity studies conducted on human liver and breast cell lines as... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759890 Thu, 03 Sep 2009 16:41:04 +0100 2759890 http://www.medworm.com/index.php?rid=2759889&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724880%26dopt%3DAbstract Authors: Gnanasekar M, Suleman FG, Ramaswamy K, Caldwell JD The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed, three were found to be membrane-associated proteins: synaptosomal associated protein 25 (SNAP25), Thy-1 cell ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759889 Thu, 03 Sep 2009 16:41:01 +0100 2759889 http://www.medworm.com/index.php?rid=2759887&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724881%26dopt%3DAbstract In this study, a novel dedifferentiated CS cell line, MS0812, was spontaneously established from mutated human embryonic muscle cells. Several features of the cell line were investigated, including growth characteristics, cytogenetics, electron microscopic features, expression of various antigenic markers and tumor formation. MS0812 has been cultured continuously for more than 3 years. The growth characteristics of MS0812 are similar to the immortalized cell lines as reported. The cell line exhibited complex karyotypes and hyperploidy, the chromosome number ranged from 50 to 158. MS0812 was positive for vimentin, desmin and muscle actin, indicating their muscle origin. With specific inductive condition, MS0812 differentiates into neural cells and adipocytes. Deletion of the p16 gene, which... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759887 Thu, 03 Sep 2009 16:40:59 +0100 2759887 http://www.medworm.com/index.php?rid=2759885&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724882%26dopt%3DAbstract In this study we investigated whether interactions between each CD3epsilon subunit play a role in the formation of the CD3 molecular complex. Our results revealed that the human CD3epsilon subunit forms a homodimer structure, which is a crucial piece of information for the elucidation of cellular signaling following TCR receptor ligation, and provide insight into our understanding of the molecular assembly of the CD3 molecular complex. PMID: 19724882 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759885 Thu, 03 Sep 2009 16:40:57 +0100 2759885 http://www.medworm.com/index.php?rid=2759884&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724883%26dopt%3DAbstract Authors: Qian CF, Yan W, Zhang JX, Shi L, Qian J, Fu Z, Kang CS, Liu N, You YP The Notch signaling pathway takes part in coordinated regulation of cell growth, survival and differentiation. Previous findings have shown that Notch1 and Delta-like-1 (DLL1) are overexpressed in many glioma cell lines and primary human gliomas. Down-regulation of DLL1 by RNA interference inhibits proliferation and induces apoptosis in multiple glioma cell lines. Our studies showed that Notch1 expression plasmid induced more expression of DLL1 in the U251MG glioma cell line. Adversely, blocking Notch1 receptors down-regulated the expression of DLL1. Both down-regulating DLL1 and blocking Notch1 receptors induced U251MG cell apoptosis and proliferation inhibition, and combining the two treatments produced st... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759884 Thu, 03 Sep 2009 16:40:55 +0100 2759884 http://www.medworm.com/index.php?rid=2759883&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724884%26dopt%3DAbstract In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase alpha-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil O staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content compared with the non-treated group. These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating alpha-calcineurin and preventing the induction... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759883 Thu, 03 Sep 2009 16:40:52 +0100 2759883 http://www.medworm.com/index.php?rid=2759882&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724885%26dopt%3DAbstract This study suggests that cholesterol overloading in hepatic L02 cells induces ERS and activates the UPR which, in part, leads to the apoptotic damage of cells. PMID: 19724885 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759882 Thu, 03 Sep 2009 16:40:49 +0100 2759882 http://www.medworm.com/index.php?rid=2759880&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724886%26dopt%3DAbstract Authors: Takeda A, Maeda M, Iseki H, Hirooka E, Shinozuka N, Koyama I Human anal canal squamous cell carcinoma (SCC) cell line has not yet been reported due to the rarity of this disease. Since cell lines to study this malignancy were not available, we attempted to establish and characterize anal canal SCC cell line from primary culture of lymph node metastasis. Six sublines were cloned and isolated from parental cells. They were designated as SaTM-1A, B, C, D, E and F. The features of the six sublines were characterized by reverse transcription-PCR, chemosensitivity test to 5-Fu and CDDP, immunohistochemistry, cDNA microarray analysis and tumorigenicity using immunodeficient mice. All sublines were proliferated in multiple layers at an average doubling time of 24.5 h. VEGF-A, -B, VEGF... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759880 Thu, 03 Sep 2009 16:40:46 +0100 2759880 http://www.medworm.com/index.php?rid=2759879&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724887%26dopt%3DAbstract Authors: Donati V, Lupi C, Alì G, Corsi V, Viti A, Lucchi M, Mussi A, Fontanini G The histologic heterogeneity of lung adenocarcinoma is well known. Many histologic subtypes have been described, and recently their prognostic and predictive value has emerged. Laser capture microdissection may aid in the isolation of cancer cells from distinct subtypes of lung adenocarcinoma, thus enabling the description of their specific molecular features. Characterization of epidermal growth factor receptor (EGFR) mutations in histologic subtypes of lung adenocarcinoma has become an important issue. The purpose of this study was to analyze EGFR mutations in exons 18-21 in single histologic subtypes of lung adenocarcinoma after laser capture microdissection. A revision and reclassification of a s... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759879 Thu, 03 Sep 2009 16:40:44 +0100 2759879 http://www.medworm.com/index.php?rid=2759878&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724888%26dopt%3DAbstract This study focused on soluble CD1d (sCD1d) by generating recombinant sCD1d proteins and assaying them in plasma using a newly established ELISA method. The amount of sCD1d proteins in plasma was significantly decreased in rheumatoid arthritis (RA) patients (55.2+/-13.3 years, mean +/-SD) compared with healthy donors (31.2+/-7.4 years). Plasma sCD1d protein levels correlated with the number of NKT cells (TCR Valpha24+ Vbeta11+CD3+) in peripheral blood mononuclear cells (r(2)=0.061). Furthermore, sCD1d proteins induced IFN-gamma production from NKT cells, but neither IL-4 nor IL-10. These findings suggest that the low plasma levels of sCD1d protein in RA patients reduce the number and thus activation of peripheral NKT cells. It is therefore hypothesized that sCD1d stimulates NKT cells and lo... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759878 Thu, 03 Sep 2009 16:40:39 +0100 2759878 http://www.medworm.com/index.php?rid=2759877&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724889%26dopt%3DAbstract Authors: Yoshimura M, Sakamoto K, Tsuruta A, Yamamoto T, Ishida K, Yamaguchi H, Nagaoka I In the present study, to investigate the effect of glucosamine, a component of glycosaminoglycans with a chondroprotective action, on articular cartilage in athletes, we looked at soccer players, who expose their joints to excessive motion and loading, and compared the levels of biomarkers for type II collagen degradation (CTX-II) and type II collagen synthesis (CPII) between soccer players and non-athlete controls, and in soccer players before and after glucosamine-administration. CTX-II (P<0.01) and CPII (P=0.08) levels were substantially elevated in soccer players compared with those in controls, indicating that cartilage metabolism (type II collagen degradation and synthesis) is increased i... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759877 Thu, 03 Sep 2009 16:40:36 +0100 2759877 http://www.medworm.com/index.php?rid=2759876&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724890%26dopt%3DAbstract Authors: Park C, Choi YW, Hyun SK, Kwon HJ, Hwang HJ, Kim GY, Choi BT, Kim BW, Choi IW, Moon SK, Kim WJ, Choi YH We isolated two phytochemical lignans, schisandrin and schisandrin C, from Schizandra chinensis Baill and investigated their anti-cancer effects in human leukemia U937 cells. Schisandrin C inhibited cell growth in a dose-dependent manner, which was associated with the induction of G1 arrest of the cell cycle and apoptosis; schisandrin did not inhibit growth. Schisandrin C induced G1 arrest was correlated with down-regulation of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, inhibition of phosphorylation of retinoblastoma protein (pRB), and up-regulation of the Cdk inhibitor p21(WAF1/CIP1). In addition, schisandrin C-induced apoptosis was associated... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759876 Thu, 03 Sep 2009 16:40:33 +0100 2759876 http://www.medworm.com/index.php?rid=2759875&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724891%26dopt%3DAbstract Authors: Pietrangelo T, Mancinelli R, Toniolo L, Cancellara L, Paoli A, Puglielli C, Iodice P, Doria C, Bosco G, D'Amelio L, di Tano G, Fulle S, Saggini R, Fanò G, Reggiani C Several studies have examined the effects of vibrations on muscle mass and performance in young healthy people. We studied the effects of vibrations on muscles of elderly male and female volunteers (65-85 years of age) diagnosed with sarcopenia. We applied mechanical vibrations locally (local vibrational training) to the thigh muscles at 300 Hz for a period of 12 weeks, starting with a session of 15 min stimulation once a week and increasing to three sessions of 15 min per week. Treated muscles displayed enhanced maximal isometric strength and increased content of fast MyHC-2X myosin. Single muscle fiber anal... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759875 Thu, 03 Sep 2009 16:40:31 +0100 2759875 http://www.medworm.com/index.php?rid=2759874&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724892%26dopt%3DAbstract In conclusion, we demonstrated that A. schlechtendalii root extract specifically targeted carcinogenic mechanisms, because Cdc25A and cyclin D1 are oncogenes that are frequently overexpressed in a variety of cancer entities and further, this extract affected microtubule function reminiscent of taxol. PMID: 19724892 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759874 Thu, 03 Sep 2009 16:40:27 +0100 2759874 http://www.medworm.com/index.php?rid=2759872&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724893%26dopt%3DAbstract This study investigated if soluble HLA-G molecules producted by the cumulus-oocyte complex (COC) are markers of oocyte maturation. sHLA-G molecule levels were analyzed using Bio-Plex assay in 152 COC supernatants obtained from 42 women and maturated by an 'in vitro maturation procedure'. The presence of sHLA-G molecules was confirmed by Western blotting technique. The results demonstrate detectable amounts of sHLA-G molecules ranging from 300 to 800 pg/ml in 14/73 (19%) COCs that generated mature oocytes and complete absence of detectable sHLA-G antigens in the supernatants of COCs that corresponded to immature oocytes. The detection of sHLA-G molecules in the COC culture supernatants corresponding to matured oocytes is proposed to be a marker to identify gametes with higher functionality.... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759872 Thu, 03 Sep 2009 16:40:24 +0100 2759872 http://www.medworm.com/index.php?rid=2759871&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724894%26dopt%3DAbstract In this study, we performed in vitro experiments with 293 cells transfected with IRS-1. These studies demonstrated that there is a dramatic change in the phosphorylation pattern of Tyr, Ser and Thr residues in IRS-1 as a function of insulin levels. Specifically, Ser and Thr residues in the C-terminal region were phosphorylated only at high insulin levels. SILAC (stable isotope labeling with amino acids in cell culture) followed by sequencing of C-terminal IRS-1 fragments by tandem mass spectrometry demonstrated that there is significant protein cleavage at these sites. These findings suggest that one of the biological roles of the C-terminal region of IRS-1 may be negative modulation of the finely coordinated insulin signaling system. Clearly, this could represent an important factor in in... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759871 Thu, 03 Sep 2009 16:40:21 +0100 2759871 http://www.medworm.com/index.php?rid=2759870&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724895%26dopt%3DAbstract Authors: Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y Chronic kidney disease (CKD) is recognized as a risk factor not only for end-stage renal disease but also for cardiovascular disease. Early detection and treatment of CKD is a likely key factor for prevention of its complications. Although genetic linkage analyses and association studies have implicated several loci and candidate genes in predisposition to CKD, the genes that underlie genetic susceptibility to this condition have remained largely unknown. The purpose of the present study was to identify genetic variants that confer susceptibility to CKD in Japanese individuals. The study population comprised 4,829 Japanese individuals (2,697 men, 2,132 women), including ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759870 Thu, 03 Sep 2009 16:40:19 +0100 2759870 http://www.medworm.com/index.php?rid=2759869&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724896%26dopt%3DAbstract Authors: Farnebo L, Jedlinski A, Ansell A, Vainikka L, Thunell LK, Grénman R, Johansson AC, Roberg K The present study was undertaken to evaluate the possibility of using a panel of proteins and single nucleotide polymorphisms (SNPs) involved in apoptosis, growth control, and DNA repair as predictive markers for cisplatin sensitivity. For this purpose the intrinsic cisplatin sensitivity (ICS) was determined in 39 cell lines derived from squamous cell carcinomas of the head and neck using a colony-forming assay. In these cell lines and in normal oral keratinocytes (NOK), the expression of epidermal growth factor receptor (EGFR), Hsp70, Bax, Bcl-2, Bcl-XL, survivin, and COX-2 was determined. Moreover, the p53, MDM2, FGFR4, XPC, XPD, XRCC1, and XRCC3 genes were analyzed for the prese... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759869 Thu, 03 Sep 2009 16:40:16 +0100 2759869 http://www.medworm.com/index.php?rid=2759868&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724897%26dopt%3DAbstract In conclusion, these results indicate that ANX2 on cell surface functions as a mediator boosting TF expression on monocytes induced by anti-beta2GPI/beta2GPI complex, which is contributed to the thrombotic events in APS. PMID: 19724897 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759868 Thu, 03 Sep 2009 16:40:14 +0100 2759868 http://www.medworm.com/index.php?rid=2759867&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724898%26dopt%3DAbstract Authors: Tahara T, Shibata T, Nakamura M, Yamashita H, Yoshioka D, Okubo M, Yonemura J, Maeda Y, Maruyama N, Kamano T, Kamiya Y, Fujita H, Nakagawa Y, Nagasaka M, Iwata M, Hirata I, Arisawa T CpG island hyper-methylation (CIHM) is one of the major events in the gastric carcinogenesis and also occurs in non-neoplastic gastric mucosa. IL-17A, -17F and MIF have a crucial role in the gastric inflammationand carcinogenesis. The CIHM status in the non-cancerous gastric mucosa, in relation to IL-17A (-197G>A, rs2275913), -17F (7488T>C, rs763780) and MIF (-173G>C and -794 tetranucleotide repeats) polymorphisms was investigated. Gastric mucosa samples were obtained from 121 cancer free subjects. CIHM of p14, p16, DAP-kinase and CDH1 genes were determined by methylation-specific polymer... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759867 Thu, 03 Sep 2009 16:40:11 +0100 2759867 http://www.medworm.com/index.php?rid=2759866&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724899%26dopt%3DAbstract Authors: Auyeung KK, Ko JK Conventional chemotherapy of liver cancer fails to provide satisfactory remission and may cause serious side effects, thus it is crucial to derive alternative treatments that effectively inhibit cancer cell growth with known mechanisms of action. In the present study, we investigated the anti-carcinogenic effects of Coptis chinensis and its major constituent, berberine, in HepG2 hepatocellular carcinoma (HCC) cells and attempted to elucidate the underlying mechanism, including involvement of the nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1). Inhibition of cell proliferation, induction of apoptosis and cell cycle arrest at the G2/M phase were observed in HepG2 cells treated with Coptis chinensis or berberine. The pro-apoptotic effects were... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759866 Thu, 03 Sep 2009 16:40:09 +0100 2759866 http://www.medworm.com/index.php?rid=2759865&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19724900%26dopt%3DAbstract Authors: Hyc A, Osiecka-Iwan A, Niderla-Bielinska J, Jankowska-Steifer E, Moskalewski S Synovial membrane consists of fibroblasts and macrophages forming the synovial lining supported by vascularized subsynovium. Each of these components may specifically react to a particular stimulus. Thus, reactions of isolated synovial cells may not correspond to that of intact tissue. We characterized the production of hyaluronan (HA) by rat synovial membrane exposed in vitro to pro- and anti-inflammatory cytokines and compared it with previous results obtained with isolated fibroblasts. Synovial membrane dissected from one knee joint served as a control to that from the opposite knee exposed to IL-1beta, TGF-beta1, TNF-alpha, IFN-gamma or IL-4 for 24 h. The HA content was determined by ELISA, and ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2759865 Thu, 03 Sep 2009 16:40:06 +0100 2759865 http://www.medworm.com/index.php?rid=2654274&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639219%26dopt%3DAbstract Authors: Bran GM, Goessler UR, Hormann K, Riedel F, Sadick H Excess scar formation occurs after dermal injury as a result of abnormal wound healing. Hypertrophic scars and keloids both represent fibrotic skin conditions which can be very difficult, even frustrating, to treat. Identification of differences between hypertrophic scars, keloids and normal scars are a prerequisite for finding the correct therapeutical concept. Despite the relatively high prevalence of keloids in the general population, the mechanisms underlying keloid formation are only partially understood. This fact is reflected in the multiple treatment modalities, of which no single treatment has proven to be widely effective. Advances in our understanding of the wound healing process reveal new pathophysiological conce... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654274 Thu, 30 Jul 2009 17:00:50 +0100 2654274 http://www.medworm.com/index.php?rid=2654273&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639220%26dopt%3DAbstract Authors: Quatresooz P, Pierard GE, Pierard-Franchimont C The clinical diagnosis of cutaneous melanoma always calls for histological confirmation. In addition to the recognition of the classic aspects of the neoplasm, immunohistochemistry is determinant, in particular in the assessment of the size of the replicative compartment. Generally, the proliferation rate is indicative of the neoplastic progression and is related to the clinical growth rate of the neoplasm. It allows to distinguish high risk melanomas showing a high growth rate from those of lower malignancy associated with a restricted growth rate. In melanoma, the recruitment and progression of neoplastic cells in the cell cycle of proliferation have lost some of their controls that are normally processed by a series of key reg... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654273 Thu, 30 Jul 2009 17:00:48 +0100 2654273 http://www.medworm.com/index.php?rid=2654272&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639221%26dopt%3DAbstract Authors: Vardatsikos G, Mehdi MZ, Srivastava AK Over the last several decades, a large body of evidence has accumulated to suggest that organo-vanadium compounds (OVC) are more potent than inorganic vanadium salts in regulating hyperglycemia and insulin-resistance in rodent models of both type I and type II diabetes. Among these OVC, vanadium (IV) oxo bis(maltolato) (BMOV) was the first to be investigated for its higher potency over inorganic vanadium salts in eliciting insulin-like properties in both in vitro and in vivo systems. While the precise molecular mechanism by which BMOV exerts its insulin-mimetic effects remains poorly defined, studies have shown that BMOV is a potent activator of several key components of the insulin signaling pathways, such as phosphatidyl-inositol 3-kina... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654272 Thu, 30 Jul 2009 17:00:43 +0100 2654272 http://www.medworm.com/index.php?rid=2654271&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639222%26dopt%3DAbstract In this study, we investigated the effects of cadmium on the expression of molecular chaperones, and of certain cell-specific proteins, in a variety of brain cell types in culture, namely primary cultures of rat cortical neurons and astrocytes, a brain capillary endothelial cell line (RB4E.B cells), and pheochromocytoma cells (PC12), induced or not to differentiate by NGF treatment. The metal induces a dose-dependent increase of Hsp70 in all cell types. Responses to the metal are cell-specific in the case of Hsc70 and Hsp90: i) in astrocytes, as well as in PC12 cells, cadmium has no significant effect; ii) in endothelial cells, an increase of both proteins is clearly observable from 20 microM cadmium; iii) both Hsp90 and Hsc70 decrease in neurons treated with high doses of cadmium. Damage ... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654271 Thu, 30 Jul 2009 17:00:41 +0100 2654271 http://www.medworm.com/index.php?rid=2654270&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639223%26dopt%3DAbstract In conclusion, our findings suggest that glutathionylation inhibits NF-kappaB activity causing reduced hepatocyte survival, which is common in several liver diseases. PMID: 19639223 [PubMed - in process] (Source: International Journal of Molecular Medicine) International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654270 Thu, 30 Jul 2009 17:00:39 +0100 2654270 http://www.medworm.com/index.php?rid=2654269&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639224%26dopt%3DAbstract In this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, COS A (MW, 10,000 Da) and COS B (MW, 1,000 Da), from 90-95% deacetylated chitosan, against V. vulnificus in vitro and in vivo. Treatment with COS A resulted in significantly higher suppressive effects on the growth of V. vulnificus than treatment with COS B. The growth of V. vulnificus was inhibited within 1 h of treatment with water-soluble COS A in concentrations ranging from 0.5 to 10 mg/ml. Additionally, treatment with COS A completely inhibited V. vulnificus-induced cytotoxicity in human intestinal epithelial INT-407 cells, while COS B did not. Furthermore, the administration of COS A (0.1-0.5 mg per mouse) significantly increased the survival period of V. vulnificus-infected mice. T... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654269 Thu, 30 Jul 2009 17:00:36 +0100 2654269 http://www.medworm.com/index.php?rid=2654268&cid=s_36720_67_f&fid=36720&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19639225%26dopt%3DAbstract In this study, BM of three secondary acute myelogenous leukemia (AML) cases with trisomy 8 and otherwise normal karyotype were evaluated. Bone marrow cells of one AML and one ALL (acute lymphoblastic leukemia) case, peripheral blood lymphocytes and human sperm, all of them with normal karyotype, served as controls. Multicolor banding (MCB) probes for chromosomes 8 and 21 were applied in suspension-FISH (S-FISH). Interestingly, in myeloid bone marrow cells chromosomes 8 (di- and trisomic) and 21 tended to co-localize with their homologue chromosome(s), rather than to be separated. Thus, the co-localization of chromosomes 8 and 21 might promote a translocation providing a selective advantage of t(8;21) cells in AML-M2. In summary, the concept that tissue specific spatial proximity of chromos... International Journal of Molecular Medicine journals http://www.medworm.com/rss/comments.php?id=2654268 Thu, 30 Jul 2009 17:00:34 +0100 2654268