MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'International Journal of Systematic and Evolutionary Microbiology' source. Thu, 09 Feb 2012 21:35:00 +0100 http://www.medworm.com/index.php?rid=5637784&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268071%26dopt%3DAbstract Authors: Sun ZB, Zhang H, Yuan XF, Wang YX, Feng DM, Wang YH, Feng YJ Abstract Strain Y4T, a Gram-negative, rod-shaped, aerobic and non-motile bacterium was isolated from Pinggu District, east Beijing in People's Republic of China. Polyphasic approach was used to determine the precise taxonomic position of strain Y4T. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain Y4T is most closely related to Luteimonas aquatic RIB1-20T (96.7%). DNA-DNA relatedness between strain Y4T and strain L. aquatic RIB1-20T is 42.5±3.9%. The predominant fatty acids are iso-C15:0, iso-C17:1ω9c and iso-C16:0. Q-8 is the major ubiquinone. The DNA G+C content of the type strain is 69.9 mol%. Based on the evidence above, strain Y4T represents a novel species within the genus Lutei... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637784 Fri, 20 Jan 2012 05:00:00 +0100 5637784 http://www.medworm.com/index.php?rid=5637783&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268072%26dopt%3DAbstract Authors: Sipiczki M Abstract Five strains of a previously unknown anamorphic, methanol-assimilating yeast species are described for which the name Candida borneonana is proposed. The strains were isolated from fruit waste collected in markets in Brunei (Borneo). The sequences of the D1/D2 domains of the large subunit rRNA genes, the ITS (internal transcribed spacer) regions and the 18S rRNA genes were identical in the isolates and differed from the corresponding sequences of all known yeast species. In a phylogenetic analysis of these sequences, the new species formed a cluster with Kuraishia species (the closest species was K. capsulata with 6% nucleotide substitutions in the D1/D2 domain). The type strain is 11-487T. It has been deposited in Centralbureau voor Schimmelcultures (U... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637783 Fri, 20 Jan 2012 05:00:00 +0100 5637783 http://www.medworm.com/index.php?rid=5637782&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268073%26dopt%3DAbstract Authors: Kurtzman CP Abstract Candida kuoi sp. nov. (NRRL Y-27208T, CBS 7267T, type strain) is described from a strain isolated from concentrated grape juice in Cape Province, South Africa. Analysis of sequences from the D1/D2 domains of the nuclear large subunit rRNA gene separated the proposed new species from Starmerella bombicola and S. meliponinorum, as well as from Candida species that are members of the Starmerella clade. Of described species, C. kuoi is most closely related to S. bombicola but can be separated from this species by its growth on D-ribose and erythritol. C. kuoi produces sophorolipids that have an open chain structure similar to C. batistae, C. riodocensis and C. stellata, which is in contrast to the closed chain sophorolipids produced by S. bombicola and C. ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637782 Fri, 20 Jan 2012 05:00:00 +0100 5637782 http://www.medworm.com/index.php?rid=5637781&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268074%26dopt%3DAbstract Authors: Rosario-Passapera R, Keddis R, Wong R, Lutz RA, Starovoytov V, Vetriani C Abstract An aerobic, alkane-oxidizing bacterium, designated strain EPR92T, was isolated from the hydrothermal fluids of a deep-sea vent on the East Pacific Rise at 9° 50' N, 104° 17' W. The cells were Gram-negative rods approximately 1.4 µm in length and 0.4 µm in width. Strain EPR92T grew between 20 ° and 40 °C (optimum 35 °C), 10 and 50 g 1(-1) NaCl (optimum 25 g 1(-1)) and pH 4.0 and pH 8.5 (optimum pH 7.5). The generation time under optimal conditions was 63 min. Strain EPR92T grew aerobically in Artificial Seawater Minimal Medium with n-alkanes as sole carbon and energy sources, and in Artificial Seawater medium supplemented with peptone and yeast extract. The predominant fatty acids were... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637781 Fri, 20 Jan 2012 05:00:00 +0100 5637781 http://www.medworm.com/index.php?rid=5637780&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268075%26dopt%3DAbstract Authors: Liu Y, Jiang JT, Xu CJ, Liu YH, Song XF, Li H, Liu ZP Abstract The bacterial strain LH2-2T was isolated from freshwater of Longhu Lake, a slightly alkaline lake (pH 8.8), in Northeast of China. Cells of strain LH2-2T were Gram-staining negative, non-spore-forming rods measuring 0.3-0.5 μm in width and 2.0-4.0 μm in length. Cells were motile by means of a single polar flagellum. Strictly aerobic and heterotrophic. Oxidase- and catalase-positive. Growth occurs at 0-36 °C (optimum, 26-34°C), pH 6.5-11 (optimum, pH 8.0-8.6) and in the presence of 0-2 % NaCl (w/v, optimum, 1%). Strain LH2-2T contained Q-8 as the major respiratory quinine. The major fatty acids were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH, 21.9 %), C17:1ω8c (18.9%), C18:1ω7c (16.4%) and C16:0 (12... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637780 Fri, 20 Jan 2012 05:00:00 +0100 5637780 http://www.medworm.com/index.php?rid=5637779&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268076%26dopt%3DAbstract Authors: Zamora L, Vela AI, Palacios MA, Sánchez-Porro C, Svensson-Stadler LA, Domínguez L, Moore E, Ventosa A, Fernández-Garayzábal JF Abstract A taxonomic study was carried out on five Gram-staining-negative, catalase- and oxidase-positive, rod-shaped bacteria isolated from the gill and liver of five diseased rainbow trout. On the basis of 16S rRNA gene sequence analysis, the trout isolates belonged to the genus Chryseobacterium, exhibiting the highest similarity with Chryseobacterium oncorhynchi 701B-08T (98.9 % sequence similarity), C. ureilyticum F-Fue-04IIIaaaaT (98.6 %), C. indologenes ATCC 29897T (98.3 %), C. jejuense JS17-8T (98.1 %) and C. gleum ATCC 35910T (98.1 %). DNA-DNA hybridization values were 99-100 % among the five isolates and 21 and 57 % between strain 687B... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5637779 Fri, 20 Jan 2012 05:00:00 +0100 5637779 http://www.medworm.com/index.php?rid=5619498&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247210%26dopt%3DAbstract Authors: Lamprinou V, Skaraki K, Kotoulas G, Economou-Amilli A, Pantazidou AI Abstract One new cyanobacterial genus Toxopsis Lamprinou & Pantazidou gen. nov. was found in fresh material of Cave 'Francthi'(Peloponnese, Greece) and isolated in cultures; additionally, ecological data of the sampling sites are provided concerning the environmental parameters [Photosynthetically Active Radiation (PAR), Temperature (T), Relative Humidity (RH)]. Morphological characters and the life cycle of the type species Toxopsis calypsus Lamprinou & Pantazidou sp. nov. were studied under LM, SEM and TEM; moreover, molecular analysis based on the 16S rRNA sequence was applied. Toxopsis calypsus is a false branched nostocalean cyanobacterium with both isopolar and heteropolar filaments bearing ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619498 Fri, 13 Jan 2012 05:00:00 +0100 5619498 http://www.medworm.com/index.php?rid=5619497&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247211%26dopt%3DAbstract Authors: Lee J, Shin NR, Lee HW, Roh SW, Kim MS, Kim YO, Bae JW Abstract A novel, Gram-negative, motile, facultative anaerobic and rod-shaped bacterium, strain A36T, was isolated from a dead ark clam found on the south coast of Korea. The isolate was catalase negative and oxidase negative. The 16S rRNA gene sequence analysis indicated that strain A36T was most closely related to Kistimonas asteriae KMD 001T, with which it shared 98.2 % similarity. Strain A36T grew optimally at 30-37 °C in the presence of 1 % (w/v) NaCl, and at pH 8.0. The major respiratory quinone was ubiquinone-9 (Q-9). Polar lipids included phosphatidylserine, phosphoethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The major fatty acids were summed features 3 (comprising C16:1 ω7c/ iso-C15 2OH) a... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619497 Fri, 13 Jan 2012 05:00:00 +0100 5619497 http://www.medworm.com/index.php?rid=5619496&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247212%26dopt%3DAbstract Authors: Jin L, Kim KK, Lee HG, Ahn CY, Oh HM Abstract A Gram-negative, aerobic, non-motile and rod and cocci-shaped bacterium, B6-12(T), was isolated from a sediment sample taken from River Geumho in South Korea. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this isolate into the α-Proteobacteria, which was most closely related to Kaistia adipata KCTC 12095(T), Kaistia dalseonensis DSM 18800(T), Kaistia geumhonensis DSM 18799(T), Kaistia granuli KCTC 12575(T), Kaistia soli KACC 12605(T) and Kaistia terrae KACC 12910(T) showing 16S rRNA gene sequence similarities to the type strains of these species of 96.2 - 99.1 %. The predominant ubiquinone was Q-10. The major fatty acids were C(18 : 0), C(18 : 1) ω7c and C(19 : 0) ω8c cyclo. The G+C content of the ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619496 Fri, 13 Jan 2012 05:00:00 +0100 5619496 http://www.medworm.com/index.php?rid=5619495&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247213%26dopt%3DAbstract Authors: Malhotra J, Anand S, Jindal S, Raman R, Lal R Abstract Taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain A648T, isolated from hexachlorocyclohexane (HCH) dumpsite located in Lucknow, India was ascertained using a polyphasic approach. A comparative analysis of partial sequence of the rpoB gene and 16S rRNA gene sequence revealed that strain A648T belongs to the genus Acinetobacter. DNA-DNA relatedness values between strain A648T and other closely related members namely Acinetobacter radioresistens DSM 6976T; Acinetobacter venetianus ATCC 31012T; Acinetobacter baumannii LMG 1041T; Acinetobacter parvus LMG 21765T; Acinetobacter junii LMG 998T and Acinetobacter soli JCM 15062T were found to be less than 8 %. The major cellular fat... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619495 Fri, 13 Jan 2012 05:00:00 +0100 5619495 http://www.medworm.com/index.php?rid=5619494&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247214%26dopt%3DAbstract Authors: Kaur I, Kaur C, Khan F, Mayilraj S Abstract A Gram-reaction-negative, non-motile bacterial strain forming straight rods and straw yellow-pigmented colonies, was subjected to a detailed polyphasic taxonomy study. The strain, designated FCS-5T, matched with most of the phenotypic properties of the genus Flavobacterium. The major fatty acids were iso-C15:0, iso-C17:03OH, C17:1ω9c and summed feature 3 (comprising iso-C15:0 2OH and/or C16:1ω7c) and only isoprenoid quinone was MK-6. The only polyamine was homospermidine and the major polar lipids were phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylserine. The G+C content of the genomic DNA was 32.4 mol%. According to 16S rRNA gene sequence analysis, strain FCS-5T belonged to the genus Flavobacterium, ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619494 Fri, 13 Jan 2012 05:00:00 +0100 5619494 http://www.medworm.com/index.php?rid=5619493&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247215%26dopt%3DAbstract Authors: Luque R, Béjar V, Quesada E, Martínez-Checa F, Llamas I Abstract A moderately halophilic bacterium (strain RS-16T) was isolated from saline soil in Rambla Salada, a Mediterranean hypersaline rambla in Murcia, south-east Spain. The strain is a Gram-negative rod, oxidase-negative and motile by peritrichous flagella. It requires NaCl and grows between 1% and 30% w/v (optimum 5-7.5% w/v) at temperatures of between 4°C and 41°C (optimum 32-37°C) and pH values of between 5 and 10 (optimum pH 7). It is chemo-organotrophic and its metabolism is respiratory with oxygen and nitrate as terminal electron acceptor. It produces acids from D-glucose and myo-inositol. It accumulates poly-β-hydroxyalkanoate granules and produces cream-coloured colonies. Its G+C content is 56.2 mol%. ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619493 Fri, 13 Jan 2012 05:00:00 +0100 5619493 http://www.medworm.com/index.php?rid=5619492&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247216%26dopt%3DAbstract Authors: Kaur J, Kaur J, Niharika N, Lal R Abstract A Gram-negative, non-motile, cream coloured and rod shaped bacterial strain named LNB2T was isolated from a brick kiln area situated in an open hexachlorocyclohexane (HCH) dumpsite at Ummari village (Lucknow, India) and was subjected to polyphasic taxonomic approach. Strain LNB2T showed 16S rRNA gene sequence similarity of 98.0 % with its closest relative Sphingomonas haloaromaticamans A175T and 97.3 % with Sphingomonas histidinilytica UM2T. Strain LNB2T formed a monophyletic group in a cluster comprising of closely related species of the genus Sphingomonas. The DNA-DNA relatedness of strain LNB2T with strain A175T and UM2T was 8.6 % and 5.6 % respectively indicating that strain LNB2T represents a novel species of the genus Sphing... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619492 Fri, 13 Jan 2012 05:00:00 +0100 5619492 http://www.medworm.com/index.php?rid=5619491&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22247217%26dopt%3DAbstract Authors: Martini M, Marcone C, Mitrovic J, Maixner M, Delic D, Myrta A, Ermacora P, Bertaccini A, Duduk B Abstract Plants of Convolvulus arvensis var. linearifolius exhibiting symptoms of undersized leaves, shoot proliferation, and yellowing, collectively defined as bindweed yellows (BY) were sampled in different regions in Europe and assessed for phytoplasma infection by PCR amplification using phytoplasma universal rRNA operon primer pairs. Positive results were obtained for all diseased plants. RFLP analysis of amplicons comprising 16S rDNA alone or 16S rDNA and 16-23S intergenic spacer region indicated that detected phytoplasmas were distinguishable from all other previously described rDNA sequences. Analysis of 16S rDNAs derived from seven selected phytoplasma strains (BY-S57/... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5619491 Fri, 13 Jan 2012 05:00:00 +0100 5619491 http://www.medworm.com/index.php?rid=5577447&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228653%26dopt%3DAbstract Authors: Didari M, Amoozegar MA, Bagheri M, Schumann P, Spröer C, Sánchez-Porro C, Ventosa A Abstract A novel Gram-positive, moderately halophilic bacterium, designated strain P4BT, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain P4BT were rod-shaped, nonmotile rods and producing ellipsoidal endospores at a central position in non-swollen sporangia. Strain P4BT was a strictly aerobic bacterium and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-12.5 % (w/v), with optimum growth occurring at 5-7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain P4BT was show... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577447 Fri, 06 Jan 2012 05:00:00 +0100 5577447 http://www.medworm.com/index.php?rid=5577446&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228654%26dopt%3DAbstract Authors: Hedberg ME, Moore ER, Svensson-Stadler L, Hörstedt P, Baranov V, Hernell O, Wai SN, Hammarström S, Hammarström ML Abstract Two new obligately anaerobic Gram-positive, saccharolytic and non-proteolytic spore-forming bacilli (strain CD3:22 and N1) are described. Strain CD3:22 was isolated from a biopsy of the small intestine of a child with celiac disease and strain N1 from the saliva of a healthy young man. The cells of both strains were observed to be filamentous with lengths of approximately 5 to >20 µm, some of them curving and with swellings. The novel organisms produced H2S, NH3, butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98 % sequence similarit... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577446 Fri, 06 Jan 2012 05:00:00 +0100 5577446 http://www.medworm.com/index.php?rid=5577445&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228655%26dopt%3DAbstract Authors: Zhang J, Ma Y, Yu H Abstract A novel Gram-positive, aerobic, rod-shaped bacterium, designated strain D94-1T, was isolated from soil selected from Liancheng county of Fujian province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain D94-1T was a member of the genus Nocardioides and closely related to Nocardioides salaries DSM 18239T (98.54 % sequence similarity), Nocardioides marinisabuli DSM 18965T (98.30 %), Nocardioides basaltis DSM 22259T (98.10 %) and Nocardioides dokdonensis KCTC 19309T (97.76 %). Phenotypic characteristics and the DNA-DNA relatedness data served to distinguish strain D94-1T from N. salaries, N. marinisabuli, N. basaltis and N. dokdonensis. The chemotaxonomic properties of strain D94-1T were consistent with those of g... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577445 Fri, 06 Jan 2012 05:00:00 +0100 5577445 http://www.medworm.com/index.php?rid=5577444&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228656%26dopt%3DAbstract Authors: Woo SG, Srinivasan S, Kim MK, Lee M Abstract Gram-staining-negative, motile bacteria, designated as MJ01T and MJ14 were isolated from a sludge sample of the Daejeon sewage disposal plant in Korea and were characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strains MJ01T and MJ14 belonged to the genus Rhodanobacter in the family Xanthomonadaceae of the Gammaproteobacteria. According to 16S rRNA gene sequences strains MJ01T and MJ14 were indistinguishable and showed 98.8% - 96.4% similarity levels with other Rhodanobacter species. Strain MJ01T exhibited relatively high levels of DNA hybridization values (89.3 %) with MJ14 and low level of DNA hybridization values (< 60 %) with other Rhodanobacter species. The DNA G+... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577444 Fri, 06 Jan 2012 05:00:00 +0100 5577444 http://www.medworm.com/index.php?rid=5577443&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228657%26dopt%3DAbstract Authors: Lee J, Whon TW, Shin NR, Roh SW, Kim J, Park SK, Kim MS, Shin KS, Lee JS, Lee KC, Kim YO, Bae JW Abstract A slightly halophilic, Gram-negative, strictly aerobic, rod-shaped, non-motile bacterial strain, designated TW15T, was isolated from the ark clam in South Korea. Growth occurred under conditions of 10-37 °C, 1-5 % (w/v) NaCl and pH 7.0-10.0. Optimal growth occurred at 25-30 °C, in the presence of 2 % (w/v) NaCl and at pH 8.0. Strain TW15T exhibited both oxidase and catalase activities. The major fatty acids of strain TW15T were summed features 8 (C18:1 ω7c and/or C18:1 ω6c), 11-methyl C18:1 ω7c. The novel strain contained ubiquinone-10 (Q-10) as the predominant isoprenoide quinone. The polar lipids of strain TW15T comprised phosphatidylcholine, phosphatidylglycero... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577443 Fri, 06 Jan 2012 05:00:00 +0100 5577443 http://www.medworm.com/index.php?rid=5577442&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228658%26dopt%3DAbstract Authors: Yoon JH, Kang SJ, Park S, Oh TK Abstract A Gram-staining-negative, non-spore-forming, facultatively anaerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, BR-18T, was isolated from marine sand on the western coast of South Korea, and subjected to a polyphasic study. Strain BR-18T grew optimally at 25 °C, at pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-18T fell within the clade comprising Mucilaginibacter species, joining the type strains of Mucilaginibacter rigui and 'Mucilaginibacter lutimaris', with which it exhibited 16S rRNA gene sequence similarity values of 95.9 and 96.6 %, respectively. Sequence similarity to the type strains of the other Mucilaginibacter species was 93.4-95.5 %... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577442 Fri, 06 Jan 2012 05:00:00 +0100 5577442 http://www.medworm.com/index.php?rid=5577441&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228659%26dopt%3DAbstract Authors: Chakravarthy SK, Ramaprasad EV, Shobha E, Sasikala C, Ramana CV Abstract Two strains (JA266T and JA333) of Gram-negative, rod shaped, phototrophic, purple nonsulfur bacteria were isolated from a freshwater fish pond and an industrial effluent. Both phototrophic and chemotrophic growth is possible by both strains. Bacteriochlorophyll a and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. Major fatty acid for both strains is C18:1ω7c (>65 %) with minor amounts of 11methyl C18:1ω7c, C16:0, C16:1ω7c and C18:0 also present. Lamellar type of intracellular photosynthetic membranes is present in both the strains. Ubiquinone-10 and rhodoquinone-10 are present as primary quinone components. Diphosphatidyl glycerol, phosphatidyl glycerol, phosp... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577441 Fri, 06 Jan 2012 05:00:00 +0100 5577441 http://www.medworm.com/index.php?rid=5577440&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228660%26dopt%3DAbstract Authors: Chen H, Jogler M, Rohde M, Klenk HP, Busse HJ, Tindall BJ, Spröer C, Overmann J Abstract 'Caulobacter leidyi' DSM 4733T has been shown to be affiliated with the family Sphingomonadaceae instead of the Caulobacteraceae, and due of its poor characterization has been omitted from the current edition of Bergey's Manual of Systematic Bacteriology and removed to limbo. We isolated a novel sphingoglycolipid containing, dimorphic prosthecate bacterium from a prealpine freshwater lake. Strain 247 and 'Caulobacter leidyi' DSM 4733T were characterized in detail. The rod-shaped cells stain Gram-negative, are aerobic, catalase- and oxidase- positive, and form a stalk or polar flagellum. Both strains grow optimally at 28-30°C, and pH 6.0-8.0. The major fatty acids are C18:1ω7c, C16:0... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577440 Fri, 06 Jan 2012 05:00:00 +0100 5577440 http://www.medworm.com/index.php?rid=5577439&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228661%26dopt%3DAbstract Authors: Lucena T, Ruvira MA, Garay E, Macián MC, Arahal DR, Pujalte MJ Abstract Strain R46T, a marine Alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. It is an aerobic chemoorganotroph, mesophilic and slightly halophilic organism, with complex ionic requirements. The phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain R46T forms a separate branch within the family Rhodobacteraceae, bearing similarities below 94.7% and 80.3%, respectively, to any other stablished species. It contains Q10 as the predominant isoprenoid quinone and C18:1 ω7c / C18:1 ω6c as the major cellular fatty acid. Phosphatidyl glycerol is the only identified polar lipid, although other lipids are also detected. The G+C content ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577439 Fri, 06 Jan 2012 05:00:00 +0100 5577439 http://www.medworm.com/index.php?rid=5577438&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228662%26dopt%3DAbstract Authors: Srinivasan S, Lee JJ, Lim S, Joe M, Kim MK Abstract A Gram-staining-positive, strictly aerobic, spherical-shaped, non-motile red-pigmented bacterial strain MK03T, was isolated from a soil sample. A polyphasic approach was applied to study the taxonomic position of strain MK03T. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MK03T belonged to the clade formed by members of the genus Deinococcus in the family Deinococcaceae. The G+C content of the genomic DNA was 64.5 mol %. Strain MK03T showed highest 16S rRNA gene sequence similarity with Deinococcus aerolatus 5516T-9T (97.4 %), Deinococcus marmoris AA-63 T (97.2 %), Deinococcus radiopugnans ATCC19172 T (97.2 %.) and Deinococcus saxicola AA-1444T (96.9 %), whereas, sequence similarity to other De... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577438 Fri, 06 Jan 2012 05:00:00 +0100 5577438 http://www.medworm.com/index.php?rid=5577437&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228663%26dopt%3DAbstract Authors: Zhang JJ, Liu TY, Chen WF, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX Abstract Three chickpea rhizobial strains (CCBAU 83963T, CCBAU 83939 and CCBAU 83908) which were identified previously as a distinctive genospecies were further studied and compared taxonomically to the related species in the genus of Mesorhizobium in the current studies. Results from SDS-PAGE of whole-cell soluble proteins showed their differences from other closely related known Mesorhizobium species. Values of DNA-DNA hybridization varied from 15.28 to 50.97% between the representative strain CCBAU 83963T and the type strains of the defined Mesorhizobium species (except for M. thiogangeticum). Representative strain CCBAU 83963T contained characteristic fatty acids similar to the components of othe... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577437 Fri, 06 Jan 2012 05:00:00 +0100 5577437 http://www.medworm.com/index.php?rid=5577436&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228664%26dopt%3DAbstract Authors: Cadez N, Raspor P, Turchetti B, Cardinali G, Ciafardini G, Veneziani G, Péter G Abstract Thirteen strains isolated from virgin olive oil or its by-products in several Mediterranean countries were found phenotypically and genetically divergent from currently recognised yeast species. Sequence analysis of the large subunit (LSU) rDNA D1/D2 domain and internal transcribed spacer regions with 5.8S rRNA gene revealed that the strains represented two novel species described as Candida adriatica sp. nov. (ZIM 2234T, CBS 12504T) and Candida molendinolei sp. nov. (DBVPG 5508T, CBS 12508T). Phylogenetic analysis based on concatenated sequences of the small subunit (SSU) rRNA gene, D1/D2 region of the LSU rRNA gene and translation elongation factor-1á gene suggested that C. adriati... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577436 Fri, 06 Jan 2012 05:00:00 +0100 5577436 http://www.medworm.com/index.php?rid=5577435&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228665%26dopt%3DAbstract Authors: Schumann P, Zhang DC, Redzic M, Margesin R Abstract A Gram-type positive, non-motile, psychrophilic actinobacterium, designated Cr8-25T, was isolated from alpine glacier cryoconite and was able to grow well over a temperature range of 1-15 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Cr8-25T belonged to the family Microbacteriaceae and showed highest 16S rRNA gene sequence similarity with Klugiella xanthotipulae 44C3T (97.0 %). However, strain Cr8-25T was differentiated from the type strain of K. xanthotipulae on the level of genomospecies by a DNA-DNA relatedness of only 37.2 %. Strain Cr8-25T contained a peptidoglycan cross-linked according to the B-type which is based on 2,4-diaminobutyric acid. The cell wall contained the sugars gala... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577435 Fri, 06 Jan 2012 05:00:00 +0100 5577435 http://www.medworm.com/index.php?rid=5577434&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228666%26dopt%3DAbstract Authors: Zhang J, Ma Y, Yu H Abstract An actinobacterial strain, designated D48T was isolated from the rhizosphere of a cypress tree collected from Mianyang in Sichuan province, China. The strain was Gram-positive, catalase-positive, oxidase-negative and non-motile, with lysine as the peptidoglycan diagnostic diamino acid and acetyl as the peptidoglycan acyl type. The predominant menaquinone was MK-9(H2); smallamounts of MK-7(H2), MK-10 (H2) and MK-6 were also present. The major fatty acids were anteiso-C15 :0, anteiso-C17: 0 and iso-C16:0. The strain underwent a rod-coccus morphological cycle, had a high mol % G + C content, was aerobic and grew between 12 and 37 °C, with optimal growth around 28 °C. On the basis of the phenotypic, chemotaxonomic analyses, 16S rRNA gene sequence... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577434 Fri, 06 Jan 2012 05:00:00 +0100 5577434 http://www.medworm.com/index.php?rid=5577433&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228667%26dopt%3DAbstract Authors: Cao YR, Jiang Y, Jin RX, Han L, He WX, Li YL, Huang XS, Xue QH Abstract A novel actinobacterium, designated strain YIM 100590T, was isolated from Panthera tigris amoyensis feces collected from Yunnan Wild Animal Park in Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 100590T is a member of the family Micrococcaceae. Its cells were coccoid to oval (0.7-1.5 µm in diameter) singly or in cluster. Growth was observed in the temperature range between 10-37°C (optimum 28°C) and pH 7.0-11.0 (optimum pH 8.0). The major fatty acids were iso-C15:0 (32.22%), anteiso-C15:0 (31.64%) and iso-C16:0 (17.38%). The peptidoglycan type was A4a, L-Lys-Gly-L-Glu. The major polar lipids were diphosphatidylglycerol (DPG), phos... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577433 Fri, 06 Jan 2012 05:00:00 +0100 5577433 http://www.medworm.com/index.php?rid=5577432&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228668%26dopt%3DAbstract Authors: Inahashi Y, Matsumoto A, Omura S, Takahashi Y Abstract An actinomycete strain K09-0627(T) was isolated from the roots of a variety of orchid collected in Okinawa Prefecture, Japan. Two actinomycete strains K11-0047(T) and K11-0057(T) were isolated from the roots of Rumex acetosa and Houttuynia cordata collected in Kanagawa Prefecture, Japan. The 16S rRNA gene sequence analyses indicated that the isolates belonged to the genus Phytohabitans and that they were closely related to each other and to Phytohabitans suffuscus K07-0523(T). The DNA-DNA hybridization relatedness values between the three isolates and P. suffuscus were below 70%. On the basis of phylogenetic analysis, DNA-DNA hybridization relatedness and phenotypic characteristics, the strains should be classified as ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577432 Fri, 06 Jan 2012 05:00:00 +0100 5577432 http://www.medworm.com/index.php?rid=5577431&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228669%26dopt%3DAbstract Authors: Srinivasan S, Park G, Yang H, Hwang S, Bae Y, Jung YA, Kim MK, Lee M Abstract A Gram-staining-positive, strictly aerobic, short-rod-shaped and non-motile strain designated MJ32T, was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. Polyphasic approach was applied to study the taxonomic position of strain MJ32T. Strain MJ32T showed the highest 16S rRNA gene sequence similarity with Gordonia hirsuta DSM 44140T (98.1 %), Gordonia hydrophobica DSM 44015T (97.0 %). Sequence similarities to other 31 Gordonia species was less than 97.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ32T belonged to the clade formed by members of the genus Gordonia in the family Gordoniaceae. The G+C content of the genomic DNA was 69... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577431 Fri, 06 Jan 2012 05:00:00 +0100 5577431 http://www.medworm.com/index.php?rid=5577430&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228670%26dopt%3DAbstract Authors: Gutierrez T, Green DH, Whitman WB, Nichols PD, Semple KT, Aitken MD Abstract A strictly aerobic, halotolerant, rod-shaped bacterium that stains Gram-negative is designated strain DG1253T. It can degrade two- and three-ring polycyclic aromatic hydrocarbons and was isolated from a laboratory culture of the marine dinoflagellate Lingulodinium polyedrum (CCAP 1121/2). The strain exhibited a narrow nutritional spectrum, preferring to utilize aliphatic and aromatic hydrocarbon compounds and small organic acids. Cells produced surface blebs and contained a single polar flagellum. The predominant isoprenoid quinone of strain DG1253T was Q-8. The fatty acid profile was dominated by C18:1 ω7c. DNA G+C content for the isolate was 63.6 ± 0.25 mol%. 16S rRNA gene sequence analysis pl... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5577430 Fri, 06 Jan 2012 05:00:00 +0100 5577430 http://www.medworm.com/index.php?rid=5546320&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199207%26dopt%3DAbstract Authors: Sakamoto M, Ohkuma M Abstract 16S rRNA gene sequence information indicated Xylanibacter oryzae has a close relationship with the genus Prevotella. To clarify the taxonomic position of X. oryzae we determined the sequence of the hsp60 gene as it represents an alternative phylogenetic marker for identification and classification of Gram-negative anaerobic rods. Based on its hsp60 gene sequence, X. oryzae was located within the genus Prevotella, indicating this species does not represent a distinct taxon at the genus level. Statistical tests, the Shimodaira-Hasegawa (SH) test and the approximately unbiased (AU) test, supported that X. oryzae is monophyletic with Prevotella species and thus belongs to the genus Prevotella. On the basis of phylogenetic findings, we propose that... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546320 Fri, 23 Dec 2011 05:00:00 +0100 5546320 http://www.medworm.com/index.php?rid=5546319&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199208%26dopt%3DAbstract Authors: Surendra V, Pant B, Korpole S, Srinivas TN, Anil Kumar P Abstract A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1T, was isolated from estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2-2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction, H2S production. The fatty acids were dominated by branched with iso- fatty acids with a high abundance of iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3OH and MK6 (64%) is the major respiratory quinone along with MK7 (34%) and phosphotidylethanolamine, two unidentified aminolipids, four unidentified phospholipids, two unidentified lipids as ma... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546319 Fri, 23 Dec 2011 05:00:00 +0100 5546319 http://www.medworm.com/index.php?rid=5546318&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199209%26dopt%3DAbstract Authors: Kudo Y, Oki K, Watanabe K Abstract Four strains isolated from a traditional Japanese non-salted pickle and initially identified as Lactobacillus delbrueckii could not be assigned to a definite subspecies because molecular identification and phenotypic characteristics did not agree with those of recognized subspecies of L. delbrueckii. Hybridization of total DNA (> 88.7% against type strains of the other three recognized subspecies), phylogenetic analyses based on 16S rRNA gene sequences, amplified fragment length polymorphism (AFLP) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling, together with phenotypic profiles indicate that the four strains form a coherent cluster and represent a novel subspecies, for which t... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546318 Fri, 23 Dec 2011 05:00:00 +0100 5546318 http://www.medworm.com/index.php?rid=5546317&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199210%26dopt%3DAbstract Authors: Ardley JK, Parker MA, De Meyer SE, Trengove RD, O'Hara GW, Reeve WG, Yates RJ, Dilworth MJ, Willems A, Howieson JG Abstract Strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes Listia angolensis (from Zambia) and Lupinus texensis (from Texas, USA). Phylogenetic analysis of the 16S rRNA gene showed that the novel strains belong to the genus Microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. The closest relative of the representative strains Lut6T and WSM3557T was M. flocculans TFBT, with 97.6-98.0 % similarity, while WSM3693T was most closely related to M. aerilata 5420S-16T, with 98.8 % similarity. Analysis of the concatenated sequences of four housekeeping gen... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546317 Fri, 23 Dec 2011 05:00:00 +0100 5546317 http://www.medworm.com/index.php?rid=5546316&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199211%26dopt%3DAbstract Authors: Hansen MJ, Bertelsen MF, Christensen H, Bojesen AM, Bisgaard M Abstract A total of 27 bacterial isolates from California sea lions and a walrus tentatively classified with the family Pasteurellaceae were further characterized by geno- and phenotypic tests. Phylogenetic analysis of partial 16S rRNA and rpoB gene sequences showed that the isolates investigated formed a monophyletic group, tentatively designated Bisgaard taxon 57. According to 16S rRNA gene sequence, the closest related validly named species was Bisgaardia hudsonensis with 95 % similarity and the closest related based on rpoB sequence comparison was Pasteurella multocida demonstrating 88.2 % similarity.All isolates of Bisgaard taxon 57 meet the phenotypic characters for the family Pasteurellaceae. Bisgaard ta... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546316 Fri, 23 Dec 2011 05:00:00 +0100 5546316 http://www.medworm.com/index.php?rid=5546315&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199212%26dopt%3DAbstract Authors: Feng Z, Zhang J, Huang X, Zhang J, Chen M, Li S Abstract A Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1T, was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, China. Strain BY-1T grew optimally at pH 7.0 and 30-35°C in the presence of 0.5% (w/v) NaCl. An analysis of 16S rRNA gene sequences revealed that strain BY-1T fell within the radiation of the genus Pseudomonas, as it showed highest sequence similarities to Pseudomonas luteola IAM 13000T (99.5 %) and Pseudomonas duriflava HR2T (97.3 %); the levels of sequence similarity with respect to other recognized Pseudomonas species were < 96.7 %. Strain BY-1T showed low DNA-DNA relatedness values with P. luteola IAM 13000T(29±3.1%) Pseudomonas duri... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546315 Fri, 23 Dec 2011 05:00:00 +0100 5546315 http://www.medworm.com/index.php?rid=5546314&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199213%26dopt%3DAbstract Authors: Wauters G, Janssens M, De Baere T, Vaneechoutte M, Deschaght P Abstract Two clinical strains, NF 296 and NF 931, present in our collection, were identified biochemically as CDC group II-i. Determination of the 16S rRNA gene sequence revealed highest similarity with Sphingobacterium mizutaii. Because these strains produced indole, whereas S. mizutaii has been described as indole negative, we investigated also the type strain and a reference strain of S. mizutaii, i.e. LMG 8340T (CCUG 15907T) and LMG 8341 (CCUG 15908), and found both strains to be indole production positive as well. These data warrant inclusion of some of the CDC group II-i strains into S. mizutaii and an emended description of this species as indole production positive and of the genus Sphingobacterium as i... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546314 Fri, 23 Dec 2011 05:00:00 +0100 5546314 http://www.medworm.com/index.php?rid=5546313&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199214%26dopt%3DAbstract Authors: Park S, Yoon JH Abstract A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, GSW-M26T, was isolated from seawater on the southern coast in Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain GSW-M26T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GSW-M26T fell within the clade comprising Jannaschia species, joining the type strain of Jannaschia seosinensis, with which it exhibited highest 16S rRNA gene sequence similarity (96.9 %). The 16S rRNA gene sequence similarity between strain GSW-M26T and members of the genus Jannaschia was in the range 93.7-95.5 %. Strain GSW-M26T contained Q-10 as the predominant ubiquin... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546313 Fri, 23 Dec 2011 05:00:00 +0100 5546313 http://www.medworm.com/index.php?rid=5546312&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199215%26dopt%3DAbstract Authors: Tan HQ, Li TT, Zhu C, Zhang XQ, Wu M, Zhu XF Abstract A bacterial strain named NS31-3(T) was isolated from the wastewater of a paper mill. The isolate was obligately anaerobic, non-pigmented, non-motile, gram-negative and short rod-shaped with 2.0-2.8 µm in width, 2.3-4.7 µm in length. The strain was able to grow on media containing 20% bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, D-xylose, L-arabinose, cellobiose, D-mannose, D-melizitose,D-raffinose, D-trehalose, D-mannitol, salicin and D-sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that anteiso-C(15:0), C(15:0) and iso-C(17:0 )3-OH were the major fatty acids, and t... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546312 Fri, 23 Dec 2011 05:00:00 +0100 5546312 http://www.medworm.com/index.php?rid=5546311&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199216%26dopt%3DAbstract Authors: Tani A, Sahin N, Kimbara K Abstract A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum. The cells of strain 23eT are Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1%) and M. marchantiae JT1T (97%), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97%. Major cellular fatty acids were C18:1 ω7c, C16:00, and C18:0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS analysis, physiological and biochemical tests ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546311 Fri, 23 Dec 2011 05:00:00 +0100 5546311 http://www.medworm.com/index.php?rid=5546310&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199217%26dopt%3DAbstract Authors: Lee HJ, Han SI, Whang KS Abstract A novel actinobacteria, designated strain BR-34(T), was isolated from rhizosphere soil of bamboo (Phyllostachys nigro var. henonis) was sampled in Damyang, Korea. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus Catenulispora. The strain contained iso-C(16:0) as major fatty acids and MK-9 (H(4)), MK-9 (H(6)) and MK-9 (H(8)) as the major isoprenoid quinone. Phylogenetic analysis of the 16S rRNA gene sequence of strain BR-34(T) was compared with those of members of the genus Catenulispora and formed a cluster with the highest sequence similarities were shown with the type strains of C. acidiphila ID139908(T) (97.4 %), C. rubra Aac-30(T) (97.3 %), C. subtropica TT 99-48(T) (97.3 %) and C. yoro... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546310 Fri, 23 Dec 2011 05:00:00 +0100 5546310 http://www.medworm.com/index.php?rid=5546309&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199218%26dopt%3DAbstract Authors: Slobodkin AI, Reysenbach AL, Slobodkina GB, Baslerov RV, Kostrikina NA, Wagner ID, Bonch-Osmolovskaya E Abstract An extremely thermophilic, anaerobic, chemolithoautotrophic bacterium (strain S95T) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Center, Pacific Ocean at a depth of 1910 m. Cells of strain S95T were oval to short Gram-negative rods, 0.5 to 0.6 µm in diameter and 1.0 to 1.5 µm in length, growing singly or in pairs. Cells were motile with a single polar flagellum. The temperature range for growth was 50-92°C, with an optimum at 74°C. The pH range for growth was 5.5-8.0, with an optimum at 7.0. Growth of strain S95T was observed at NaCl concentrations ranging from 1.5 to 3.5% (w/v). Strain S95T grew anaerobically w... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546309 Fri, 23 Dec 2011 05:00:00 +0100 5546309 http://www.medworm.com/index.php?rid=5546308&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199219%26dopt%3DAbstract Authors: Zhao B, Chen S Abstract A Gram-positive, obligately anaerobic, slender to flexible rod-shaped, and motile bacterium, strain SC/BZ-SP2T, was isolated from a mixed alkaline water and sediment of Soap Lake, Washington State, USA. Strain SC/BZ-SP2T formed salmon to pink, was alkaliphilic, and grew at pH35 7.5-10.5 with an optimum at pH 9.7. Growth occurred at temperatures of 8-40°C (optimum at 35-37°C) and at the total Na+ ions concentrations of 0.35-1.38 M (optimum at 0.44-0.69 M). This organism utilized L-arabinose, D-ribose, D-xylose, D-fructose, D-mannose, D-galactose, D-cellobiose, maltose, sucrose, trehalose, sorbitol, xylan, malate, and yeast extract as the carbon and energy sources. Best growth was observed with L-arabinose, D-cellobiose, maltose, and trehalose. Majo... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546308 Fri, 23 Dec 2011 05:00:00 +0100 5546308 http://www.medworm.com/index.php?rid=5546307&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199220%26dopt%3DAbstract Authors: Nie GX, Ming H, Li S, Zhou EM, Cheng J, Tang X, Feng HG, Tang SK, Li WJ Abstract A novel actinomycete strain, designated YIM 75904T, was isolated from a soil sample collected from dry-hot river valley in Dongchuan county, Yunnan province, south-west China. Its taxonomic status was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences clearly showed that the isolate YIM 75904T formed a distinct clade within the genus Amycolatopsis, and was closely related to Amycolatopsis sacchari K24T (99.3% similarity). Strain YIM75904T had a type-IV cell wall lacking of mycolic acids. Organism had MK-9(H4) as the predominant menaquonine, contained meso-diaminopimelic acid (meso-DAP), galactose, glucose, and arabinose as cell wall components, iso-C1... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546307 Fri, 23 Dec 2011 05:00:00 +0100 5546307 http://www.medworm.com/index.php?rid=5546306&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199221%26dopt%3DAbstract Authors: Lee HW, Shin NR, Lee J, Roh SW, Whon TW, Bae JW Abstract A novel Gram-negative, facultative anaerobic, motile and rod-shaped bacterial strain, LHW37T, belonging to the class Gammaproteobacteria, was isolated from a dead ark clam in the South coast of Korea. Strain LHW37T grew optimally at 37 °C in 2 % (w/v) NaCl and pH 7.0-8.0. The predominant cellular fatty acids were C18:1 ω7c and C16:1ω7c/iso-C15 2-OH. The major isoprenoid quinone was ubiquinone-8 (Q-8). The dominant polar lipids were phosphatidylenthanolamine and phosphatidylglycerol. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this novel strain was most closely related to the type strain of Neptunomonas japonica, with a 16S rRNA gene sequence similarity of 97.1 %. The genomic DNA G+C co... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546306 Fri, 23 Dec 2011 05:00:00 +0100 5546306 http://www.medworm.com/index.php?rid=5546305&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199222%26dopt%3DAbstract Authors: Margesin R, Spröer C, Zhang DC, Busse HJ Abstract Two Gram-negative, psychrophilic bacterial strains, Cr4-12T and Cr4-35T, isolated from alpine glacier cryoconite, were characterized using a polyphasic approach. These isolates contained ubiquinone Q-8 as the sole quinone, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0 as the dominant cellular fatty acids, putrescine and 2-hydroxyputrescine as the major polyamines, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The DNA G+C contents of strains Cr4-12T and Cr4-35T were 61.3 and 60.7 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Polaromonas. The 16S rRNA gene sequence similarity b... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546305 Fri, 23 Dec 2011 05:00:00 +0100 5546305 http://www.medworm.com/index.php?rid=5546304&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199223%26dopt%3DAbstract Authors: Zhang J, Gu T, Zhou Y, He J, Zheng LQ, Li WJ, Huang X, Li SP Abstract A salmon-red pigmented bacterial strain designated M-8T was isolated from a polluted farmland soil sample in China and was characterized using polyphasic taxonomic approach. Strain M-8T was Gram-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 20-37 °C, pH 5.0-10.0 and 0-2 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-8T belongs to the genus Terrimonas. The sequence similarities of 16S rRNA gene between M-8T and three recognized Terrimonas type strains Terrimonas ferruginea KACC 11310T, Terrimonas aquatica LMG 24825T and Terrimonas lutea KACC 13047T were 97.1 %, 96.3 % and 95.3 %, respectively. The predominant respiratory quinone was ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546304 Fri, 23 Dec 2011 05:00:00 +0100 5546304 http://www.medworm.com/index.php?rid=5546303&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199224%26dopt%3DAbstract Authors: Kämpfer P, Schäfer J, Lodders N, Martin K Abstract A Gram-positive rod shaped bacterium (01-GI-001T) isolated from a wall colonized with moulds was studied for its taxonomic position. The 16S rRNA gene sequence analysis showed, that the isolate clearly grouped into the Microbacterium cluster. On the basis of pairwise comparisons of the 16S rRNA gene sequences, isolate 01-Gi-001T was most closely related to the type strains of Microbacterium hydrocarbonoxydans (98.8%), Microbacterium profundi (98.7%), Microbacterium phyllosphaerae (98.3%) and Microbacterium foliorum (98.1%). The diagnostic diamino acid of the peptidoglycan was ornithine. The detected major menaquinones were MK-13 and MK-12. The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phos... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546303 Fri, 23 Dec 2011 05:00:00 +0100 5546303 http://www.medworm.com/index.php?rid=5546302&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199225%26dopt%3DAbstract Authors: Lata P, Lal D, Lal R Abstract AbstractA Gram-staining-negative, strictly aerobic, yellow-coloured bacterial strain designated DS-12T, was isolated from hexachlorocyclohexane (HCH) contaminated soil in Lucknow, Uttar Pradesh, India. A polyphasic approach was applied to study the taxonomic position of strain DS-12T. Strain DS-12T showed highest 16S rRNA gene sequence similarity with Flavobacterium ceti CCUG 52969T (94.2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-12T belonged to a clade represented by the genus Flavobacterium. Strain DS-12T produced flexirubin type pigments but gliding motility was not observed. The major fatty acids of strain DS-12T were iso C15:0 (48.0 %), summed feature 9 (comprising iso C17:1 ω9c and/or C 16:0 10 Met... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546302 Fri, 23 Dec 2011 05:00:00 +0100 5546302 http://www.medworm.com/index.php?rid=5546301&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22199226%26dopt%3DAbstract Authors: Santhanam R, Okoro CK, Rong X, Huang Y, Bull AT, Weon HY, Andrews BA, Asenjo JA, Goodfellow M Abstract The taxonomic position of a Streptomyces strain isolated from an extreme hyper-arid soil sample collected from the Atacama Desert was determined using a polyphasic approach. The strain, isolate C60 T, had chemical and morphological features typical of the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA Streptomyces gene tree together with the type strain of Streptomyces radiopugnans. The two strains were distinguished readily using a combination of phenotypic properties and by a DNA:DNA relatedness value of 23.17 (±0.95)%. On the basis of these genotypic and phenotypic data it is proposed that isolate C60 T (CGMCC NO 4.7018 T = KACC 15492 T) be cla... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5546301 Fri, 23 Dec 2011 05:00:00 +0100 5546301 http://www.medworm.com/index.php?rid=5530463&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180608%26dopt%3DAbstract In this study, two strains SCSIO 10187T and SCSIO 10197 were isolated from a sediment sample collected from the South China Sea and characterized by using a polyphasic approach. Growth was observed in the temperature range between 15-35 °C (optimum 28 °C) and pH 5.0-8.0 (optimum pH 6.0). Based on 16S rRNA gene sequence analysis, the strains were identified as members of the genus Rhodococcus. Phylogenetic analysis showed that the two strains clustered together and the 16S rRNA gene sequence similarities between them and other Rhodococcus members were 93.2-97.7 %. The menaquinone type was MK-8(H2). Major cellular fatty acids were C16 : 0, C18 : 1ω9c, C17 : 0, 10-methyl C18 : 0, C18 : 0, C19:0 and C17:1ω8c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphat... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5530463 Fri, 16 Dec 2011 05:00:00 +0100 5530463 http://www.medworm.com/index.php?rid=5530462&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180609%26dopt%3DAbstract Authors: Jabari L, Gannoun H, Cayol JL, Hedi A, Sakamoto M, Falsen E, Ohkuma M, Hamdi M, Fauque G, Ollivier B, Fardeau ML Abstract A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic bacterium staining Gram-positive but showing the typical cell wall structure of Gram-negative bacteria, was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. The strain designated LIND7HT, grew at 20-45 °C (optimum, 35-40 °C) and at pH 5-8.5 (optimum pH 6.5-7.5). It did not require NaCl for growth, but was able to grow in the presence of up to 2 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain LIND7HT used cellobiose, glucose, lactose, mannose, maltose, peptone, rhamnos... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5530462 Fri, 16 Dec 2011 05:00:00 +0100 5530462 http://www.medworm.com/index.php?rid=5530461&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180610%26dopt%3DAbstract Authors: A S, K R, Evv R, Ch S, Ch RV Abstract An oval to rod shaped, Gram-stain-negative, phototrophic bacterium designated as strain JA738T was isolated from a sediment sample collected from a pink pond. Strain JA738T was non-motile and has vesicular type intracellular photosynthetic membranes. Bacteriochlorophyll a and carotenoids of the spheroidene series were present as the major photosynthetic pigments. Strain JA738T requires thiamine and pantothenate for growth. C18:1ω7c, C18:1ω5c, C18:0, C18:1ω7c11-methyl are the major cellular fatty acids and contain minor amounts of C10:03OH and C16:0. Q-10 is the major quinone and contain phosphatidyl glycerol, phosphatidyl ethanolamine and two unidentified sulfolipids (SL1,2) as major polar lipids. Phylogenetic analysis on the basis ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5530461 Fri, 16 Dec 2011 05:00:00 +0100 5530461 http://www.medworm.com/index.php?rid=5530460&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180611%26dopt%3DAbstract Authors: Zhang Y, Zhang J, Fan L, Pang H, Xin YH, Zhang X Abstract A Gram-positive-staining bacterium, designated strain Y16T, was isolated from a soil sample of Yunnan, China, and subjected to a polyphasic taxonomic study. The isolate grew optimally at 25-30 °C and pH 6.0-9.0. The strain could grow at 3% NaCl. Strain Y16T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-9(H4), MK-9(H2) as the predominant menaquinones. Major fatty acid methyl esters were iso-C15:0, anteiso-C15:0, C16:0 and anteiso-C17:0. These chemotaxonomic characteristics suggested that the organism belonged to the genus Actinoplanes. Strain Y16T shared 98.7%, 98.3% and 97.9% 16S rRNA gene sequence similarity with that of Actinoplanes deccanensis ATCC 21983T, Actinoplanes abujensis DSM 45518T... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5530460 Fri, 16 Dec 2011 05:00:00 +0100 5530460 http://www.medworm.com/index.php?rid=5530459&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22180612%26dopt%3DAbstract Authors: Choi HA, Lee SS Abstract A Gram-staining-negative, strictly aerobic, rod-shaped, non-motile strain designated 2-1-2T, was isolated from PCE/TCE contaminated soil in Suwon, South Korea. A polyphasic approach was applied to study the taxonomic position of strain 2-1-2T. Strain 2-1-2T showed highest 16S rRNA gene sequence similarity with Sphingobacterium daejeonense TR6-04T (97.9 %) and Sphingobacterium mizutaii ATCC 33299T (97.1 %); sequence similarity to other Sphingobacterium species was less than 93.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 2-1-2T belonged to the clade formed by members of the genus Sphingobacterium in the family Sphingobacteriaceae. The G+C content of the genomic DNA was 36.6 mol %. Strain 2-1-2T showed the typical ch... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5530459 Fri, 16 Dec 2011 05:00:00 +0100 5530459 http://www.medworm.com/index.php?rid=5510755&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140176%26dopt%3DAbstract Authors: Slobodkina GB, Reysenbach AL, Panteleeva A, Kostrikina N, Wagner I, Bonch-Osmolovskaya E, Slobodkin AI Abstract Summary. A moderately thermophilic, anaerobic, dissimilatory Fe(III)-reducing bacterium (strain S3R1T) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Center, Pacific Ocean at a depth of about 2150 m. Cells of strain S3R1T were ovals to short rods with a single polar flagellum, Gram-stain-negative, 0.5 to 0.6 µm in diameter and 0.8 to 1.3 µm in length, growing singly or in pairs. The temperature range for growth was 36-62oC, with an optimum at 50oC. The pH range for growth was 5.5-7.5, with an optimum at 6.5. Growth of strain S3R1T was observed at NaCl concentrations ranging from 1.0 to 5.0% (w/v) with an optimum at 2... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510755 Fri, 09 Dec 2011 05:00:00 +0100 5510755 http://www.medworm.com/index.php?rid=5510753&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155757%26dopt%3DAbstract Authors: Oren A Abstract The halophilic Archaea of the family Halobacteriaceae (36 genera with 129 species with standing in the nomenclature as of November 2011) provide an excellent example of how changing concepts on prokaryote taxonomy and development of new methods have influenced the way the taxonomy of a single group of prokaryotes is treated. This review gives an overview of the taxonomy of the Halobacteriaceae, showing the impact of methods of phenotypic characterization, numerical taxonomy, chemotaxonomy and especially polar lipid analysis, 16S rRNA sequence comparisons, multilocus type analysis and comparative genomics on their classification. PMID: 22155757 [PubMed - as supplied by publisher] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510753 Fri, 09 Dec 2011 05:00:00 +0100 5510753 http://www.medworm.com/index.php?rid=5510752&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155758%26dopt%3DAbstract Authors: Kämpfer P, Arun AB, Young CC, Rekha PD, Martin K, Busse HJ, Chen WM Abstract A Gram-negative, non-spore forming rod (CC-LN1-12T) was isolated from soil samples of Green Island (Lutao), Taiwan and studied for its taxonomic allocation. 16S rRNA gene sequence analysis showed that isolate CC-LN1-12T was grouped into the Microbulbifer cluster, with the highest similarities to the type strain of Microbulbifer okinawensis (97.9%), Microbulbifer maritimus (97.7%) and Microbulbifer donghaensis (97.7 %), similarities to all other Microbulbifer species was lower than 96.8%. The polyamine pattern contains the major compounds spermidine and cadaverine. The fatty acids profile comprising the major fatty acids iso C15:0, iso C17:1 ω9c, C18:1 ω7c beside major hydroxylated fatty acids i... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510752 Fri, 09 Dec 2011 05:00:00 +0100 5510752 http://www.medworm.com/index.php?rid=5510751&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155759%26dopt%3DAbstract Authors: Fu Y, Li Q, Liu K, Xu Y, Wang Y, Jiao N Abstract Abstract: A Gram-positive, motile, short-rod-shaped strain, designated JLT1488T, was isolated from the South China Sea, and was investigated using a polyphasic taxonomic approach. The peptidoglycan type determined for strain JLT1488T was A4a with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of L-Lys-L-Glu. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown glycolipid and an unknown phospholipid. The only detected menaquinone was MK-8(H4), the major fatty acids were summed feature 8 (18:1ω7c/18:1ω6c) (41.48%), C16:0 (29.63%) and summed feature 3 (16:1ω7c/16:1ω6c) (8.40%); significant amounts of C12:0 3O... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510751 Fri, 09 Dec 2011 05:00:00 +0100 5510751 http://www.medworm.com/index.php?rid=5510750&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155760%26dopt%3DAbstract Authors: Verma P, Pandey PK, Gupta AK, Seong CN, Park SC, Choe HN, Baik KS, Patole MS, Shouche YS Abstract We have carried out a polyphasic taxonomic characterization of validly published type strains of species, Bacillus beijingensis DSM 19037T and Bacillus ginsengi DSM 19038T that were phylogenetically closely related to Bhargavaea cecembensis LMG 24411T. All the strains are Gram-positive, non-motile, moderately halotolerant and non-spore forming. 16S rRNA gene sequence analyses showed that these constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. The percentage similarity on the basis of amino acid sequences deduced from the partial gyrB gene nucleotide sequences of these three type strains was 96.1-92.7 %. Phylogenetic trees based on the 16S rRNA... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510750 Fri, 09 Dec 2011 05:00:00 +0100 5510750 http://www.medworm.com/index.php?rid=5510749&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155761%26dopt%3DAbstract Authors: De Meyer SE, Willems A Abstract Gram-negative, rod-shaped bacteria were isolated from Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia root nodules. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100 - 97%), belonging to the Alphaproteobacteria class and Bradyrhizobiaceae family. The closest relative for LMG 26383T, LMG 26379T and LMG 26381T were respectively, Bosea thiooxidans (99.6%), Bosea eneae (98.3%) and Bosea minatitlanensis (99.0%). Chemotaxonomic data, including the major fatty acid profiles supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridisations, physiological and bioc... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510749 Fri, 09 Dec 2011 05:00:00 +0100 5510749 http://www.medworm.com/index.php?rid=5510748&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22155762%26dopt%3DAbstract Authors: Lipski A, Kämpfer P Abstract Two groups of Gram-negative, aerobic bacterial strains previously isolated from experimental biofilters were investigated to clarify their taxonomic position. Based on their 16S rRNA gene sequences these isolates formed two distinct groups within the genus Aquamicrobium. The sequence similarities of the isolates to the type strains of Aquamicrobium species were below 98.3%. The presence of ubiquinone-10, the predominant fatty acid 18:1 cis11, a polar lipid pattern with phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and ornithine lipid was in accord with the characteristics of this genus. The results of DNA-DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and pheno... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510748 Fri, 09 Dec 2011 05:00:00 +0100 5510748 http://www.medworm.com/index.php?rid=5510781&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140150%26dopt%3DAbstract Authors: Williams TJ, Lefèvre CT, Zhao W, Beveridge TJ, Bazylinski DA Abstract A marine, magnetotactic bacterium, designated strain MMS-1, was isolated from mud and water from a salt marsh in Woods Hole, Massachusetts, after enrichment in defined oxygen concentration/redox gradient medium. MMS-1 is an obligate microaerophile capable of chemoorganoheterotrophic and chemolithoautotrophic growth. Optimal growth occurred at pH 7.0 and between 24-26 °C. Chemolithoautotrophic growth occurs with thiosulfate as the electron donor and autotrophic carbon fixation is via the Calvin-Benson-Bassham cycle. The G + C content of the DNA is 47.2 mol%. Cells of MMS-1 are Gram-negative, and morphologically variable, with shapes that range from that of a lima bean to fully helical. Cells are motile ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510781 Fri, 02 Dec 2011 05:00:00 +0100 5510781 http://www.medworm.com/index.php?rid=5510780&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140151%26dopt%3DAbstract Authors: Pérez-Rodríguez I, Grosche A, Massenburg L, Starovoytov V, Lutz RA, Vetriani C Abstract A novel hyperthermophilic, anaerobic, chemolithoautotrophic bacterium, designated strain HB-8T, was isolated from the tube of Alvinella pompejana tubeworms collected from the wall of an actively venting sulfide structure on the East Pacific Rise at 13° N. The cells were Gram-negative rods approximately 1-1.5 µm in length and 0.5 µm in width. Strain HB-8T grew between 65 and 80 °C (optimum 75 °C), 15 and 35 g NaCl l-1 (optimum 30 g l-1) and pH 4.5 and 8.5 (optimum pH 6.0). Generation time under optimal conditions was 26 min. Growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Nitrate and sulfur were used as electron acc... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510780 Fri, 02 Dec 2011 05:00:00 +0100 5510780 http://www.medworm.com/index.php?rid=5510779&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140152%26dopt%3DAbstract Authors: Rezgui R, Maaroufi A, Fardeau ML, Ben Ali Gam Z, Cayol JL, Ben Hamed S, Labat M Abstract A strictly anaerobic, halotolerant and thermotolerant strain designated C5BELT was isolated from storage tanks holding wastes generated by the recycling of discarded motor oils located in north Tunisia. Cells of strain C5BELT stained Gram-positive, were motile by laterally inserted flagella, straight, and spore-forming. Their two major fatty acids were iso-C15:0 (60.1 %) and iso-C15:0 dimethyl acetal (22.0 %). Growth was observed at temperatures ranging from 25 to 55 °C (optimum 40 °C) and at pH 6 to 9 (optimum 7.5). The salinity range for growth was 0-100 g l-1 of NaCl (optimum 5 g l-1). Yeast extract is required for growth. Strain C5BELT was heterotrophic, able to use glucose, pyru... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510779 Fri, 02 Dec 2011 05:00:00 +0100 5510779 http://www.medworm.com/index.php?rid=5510778&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140153%26dopt%3DAbstract Authors: Zhu WY, Zhao LX, Zhao GZ, Duan XW, Qin S, Li J, Xu LH, Li WJ Abstract A novel endophytic actinomycete, designated YIM 68255T, was isolated from healthy leaves of Camptotheca acuminata Decne. collected in Yunnan province, south-west China and characterized by using a polyphasic approach. The strain formed well-developed substrate mycelia, but no aerial mycelium. It grew at 10-45 °C, pH 5-10 (optimum pH 7) and 0-3% (w/v) NaCl. The DNA G+C content was 73.0 mol%. Phylogenetic analyses showed that strain YIM 68255T belongs to the genus Plantactinospora. However, it exhibited some differences from Plantactinospora mayteni YIM 61359T and the level of DNA-DNA relatedness was 42.7 ± 1.3%. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510778 Fri, 02 Dec 2011 05:00:00 +0100 5510778 http://www.medworm.com/index.php?rid=5510777&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140154%26dopt%3DAbstract Authors: Ara I, Tsetseg B, Daram D, Suto M, Ando K Abstract A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0264T that formed pale to moderate yellowish brown colonies and branched substrate mycelium was studied in detail for its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0264T was grouped into the genus Cryptosporangium, being most closely related to Cryptosporangium aurantiacum (97.9 %), Cryptosporangium minutisporangium (97.7 %), Cryptosporangium arvum (97.2 %) and Cryptosporangium japonicum (96.8 %). Chemotaxonomic data [menaquinone MK-9(H6) with minor amounts of MK-9(H8) and MK-9(H4); fatty acid with major amounts of iso-C16:0, C18:1 cis 9 and C17:0 10-methyl; the phospholipid profile contains phosphatidylethanol... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510777 Fri, 02 Dec 2011 05:00:00 +0100 5510777 http://www.medworm.com/index.php?rid=5510776&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140155%26dopt%3DAbstract Authors: Wang J, Zhang J, Ding K, Xin Y, Pang H Abstract A Gram-negative, rod-shaped bacterial strain, designated F3T, was isolated from a saline soil sample in China and studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F3T was affiliated within the genus Brevundimonas, with Brevundimonas kwangchunensis KSL-102T (98.4%) and Brevundimonas alba DSM 4736T (98.2%) as its closest relatives. Strain F3T contained Q-10 as the predominant ubiquinone and C18:1ω7c, C17:1ω8c and C16:0 as the major fatty acids. The DNA G+C content was 66.7 mol%. DNA-DNArelatedness levels between strain F3T and closely related Brevundimonas species were below 22%. On the basis of the phenotypic characteristics and genotypic distinctiven... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510776 Fri, 02 Dec 2011 05:00:00 +0100 5510776 http://www.medworm.com/index.php?rid=5510747&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22156798%26dopt%3DAbstract Authors: Abstract This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission. PMID: 22156798 [PubMed - in process] (Source: Internationa... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510747 Thu, 01 Dec 2011 05:00:00 +0100 5510747 http://www.medworm.com/index.php?rid=5510746&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22156799%26dopt%3DAbstract Authors: Lindström K, Young JP PMID: 22156799 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510746 Thu, 01 Dec 2011 05:00:00 +0100 5510746 http://www.medworm.com/index.php?rid=5510764&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140167%26dopt%3DAbstract Authors: Kim H, Park DS, Oh HW, Lee KH, Chung DH, Park HY, Park HM, Bae KS Abstract Strains RU-16T, RU-28, RU-04T and PU-02T were isolated from the gut of an insect, Gryllotalpa africana. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belonged to the family Microbacteriaceae. All the strains were most closely related to Curtobacterium ginsengisoli DCY26T (below 97% 16S rRNA gene sequence similarity). These isolates were Gram-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. Their chemotaxonomic properties included MK-11 as the major respiratory quinone, ornithine as the cell-wall diamino acid, acetyl as the acyl type of the peptidoglycan, cyclohexyl-C17:0 as the major fatty acid and phosphatidylglycerol and diphosphat... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510764 Fri, 25 Nov 2011 05:00:00 +0100 5510764 http://www.medworm.com/index.php?rid=5510763&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140168%26dopt%3DAbstract Authors: Srinivas A, Rahul K, Sasikala C, Subash Y, Ramprasad EV, Ramana CV Abstract A Gram-stain-positive, oxidase negative, starch hydrolyzing, actinobacterium (JC82T) was isolated from a soda lake, Lonar, India. Based on 16S rRNA gene sequence similarity studies, strain JC82T belongs to the genus Georgenia and is most closely related to Georgenia muralis 1A-CT (96.8%) and other members of the genus Georgenia (<96.5%). The DNA G+C content of strain JC82T is 73.4 mol%. The cell-wall amino acids are alanine, glutamic acid and lysine with peptidoglycan type A4α. Polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unidentified lipid (L1) and an unidentified glycolipid (GL3). The predominant isoprenoid quinone i... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510763 Fri, 25 Nov 2011 05:00:00 +0100 5510763 http://www.medworm.com/index.php?rid=5510762&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140169%26dopt%3DAbstract Authors: Seki T, Matsumoto A, Shimada R, Inahashi Y, Omura S, Takahashi Y Abstract Two bacterial strains, KV-962T and KV-963, were isolated from soil collected from a field in Japan. They are both Gram-positive, non-spore-forming, short rod-shaped and motile bacteria. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains are related to Conexibacter woesei DSM 14684T, with similarity values of 98.6% and 98.5%, respectively. These strains possessed MK-7(H4) as a menaquinone and contained C18:1 ω9c, C17:1 ω6c and iso-C16:0 as the major fatty acids. On the basis of genotypic and phenotypic characteristics, strain KV-962T and KV-963 are indicated as a novel species of the genus Conexibacter, for which the name Conexibacter arvalis sp. nov. has been propos... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510762 Fri, 25 Nov 2011 05:00:00 +0100 5510762 http://www.medworm.com/index.php?rid=5510761&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140170%26dopt%3DAbstract Authors: Kim SJ, Tamura T, Hamada M, Ahn JH, Weon HY, Park IC, Suzuki KI, Kwon SW Abstract Strain SMC46T, a Gram-positive, aerobic, non-motile, short rod-shaped bacterium, was isolated from spent mushroom compost sample collected in the Suwon region, South Korea. 16S rRNA gene sequence analysis revealed that strain SMC46T was a member of the family Microbacteriaceae, however, formed an independent branch separate from other genera within the family Microbacteriaceae. Sequence similarity levels between strain SMC46T and other members of the family Microbacteriaceae were below 97%, showing the highest sequence similarities with Frigoribacterium faeni and Frondihabitans australicus (both, 97.0 %). Some chemotaxonomic properties of strain SMC46T were consistent with those of family Mic... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510761 Fri, 25 Nov 2011 05:00:00 +0100 5510761 http://www.medworm.com/index.php?rid=5510760&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140171%26dopt%3DAbstract Authors: Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J Abstract Despite recent advances in commercially optimized identification systems, bacterial identification remains a challenging task in many routine microbiological laboratories, especially in situations where taxonomically novel isolates are involved. The 16S rRNA gene has been used extensively for this task when coupled with a well-curated database, such as EzTaxon, containing sequences of type strains of prokaryotic species with validly published names. Although the EzTaxon database has been widely used for routine identification of prokaryotic isolates, sequences from uncultured prokaryotes have not been considered. Here, we formally introduce the next generation database, name... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510760 Fri, 25 Nov 2011 05:00:00 +0100 5510760 http://www.medworm.com/index.php?rid=5510759&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140172%26dopt%3DAbstract Authors: Shin NR, Roh SW, Kim MS, Yun B, Whon TW, Kim YO, Bae JW Abstract A novel Gram-negative, aerobic, non-motile and rod-shaped bacterium, strain M97T, was isolated from a marine sediment of cage-cultured ark clam farm in the South coast of Korea. Positive for oxidase and catalase. Optimal growth occurred at 37 °C, in 1-2 % (w/v) NaCl and at pH 7-8. The main cellular fatty acids were C16:0, C18:1 ω7c, C12:0 3OH and cyclo-C19:0 ω8c. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unknown aminolipid and several unknown lipids. The predominant respiratory quinone was ubiquinone-10 (Q-10). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain M97T belongs to the genus Tropicimonas with the highest sequence simil... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510759 Fri, 25 Nov 2011 05:00:00 +0100 5510759 http://www.medworm.com/index.php?rid=5510758&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140173%26dopt%3DAbstract Authors: Zhou EM, Yang LL, Song ZQ, Yu TT, Nie GX, Ming H, Zhou Y, Tang SK, Li WJ Abstract A novel Gram-positive, aerobic, spore-forming, thermophilic actinomycete, designated strain YIM 77521T, was isolated from a sandy soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. The strain formed branched substrate mycelia and no fragmentation was found. Masses of short, straight or irregular spore chains of 3 to 8 warty ornamented spores were borne from aerial mycelia. The strain contained meso-diaminopimelic acid in the cell wall, and the whole-cell sugars contained mannose, galactose, glucose and ribose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylmethylet... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510758 Fri, 25 Nov 2011 05:00:00 +0100 5510758 http://www.medworm.com/index.php?rid=5510757&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140174%26dopt%3DAbstract Authors: Xu XX, Wang HL, Lin HP, Wang C, Qu Z, Xie QY, Ruan JS, Hong K Abstract Strain 211020T was isolated from rhizosphere soil of Excoecaria agallocha in a mangrove, Hainan, China. The strain produced longitudinal pair spores branching from aerial hyphae. The 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Microbispora, exhibiting the highest 16S rRNA gene sequence similarity (98.75%) to M. corallina JCM 10267T with a low DNA-DNA relatedness value (13±0.6%). The isolate contained meso-A2pm as the diagnostic diamino acid but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H0) and the major fatty acids were iso-C16:0 (37.5 %), iso-C15:0 (16.82 %) and C17:0 (8.88 %). The phospholipid profile comprised of phosph... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510757 Fri, 25 Nov 2011 05:00:00 +0100 5510757 http://www.medworm.com/index.php?rid=5510756&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140175%26dopt%3DAbstract Authors: Prakash O, Green SJ, Jasrotia P, Overholt WA, Canion A, Watson DB, Brooks SC, Kostka JE Abstract Bacterial strains 2APBS1T and 116-2 were isolated from the subsurface of a nuclear legacy waste site where sediments are co-contaminated with large amounts of acidity, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first members of the Rhodanobacter genus shown to be capable of complete denitrification. Cells of strain 2APBS1T and 116-2 were Gram negative, non-spore-forming, rods, 3-5 micro;m long and 0.25-0.5 µm in diameter. The isolates were facultative anaerobes, and had temperature and pH optima for growth at 30°C and pH 6.5, respectively, and could tolerate up to 2.0 % NaCl... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510756 Fri, 25 Nov 2011 05:00:00 +0100 5510756 http://www.medworm.com/index.php?rid=5510754&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140177%26dopt%3DAbstract Authors: Denman S, Brady C, Kirk S, Cleenwerck I, Venter S, Coutinho T, de Vos P Abstract A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of Acute Oak Decline (AOD) in Britain were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus Brenneria, family Enterobacteriaceae, and are most closely related to Brenneria rubrifaciens (97.6 % sequence similarity). MLSA based on four housekeeping genes (gyrB, rpoB, infB and atpD) confirmed their position within the genus Brenneria, while DNA-DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phen... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510754 Fri, 25 Nov 2011 05:00:00 +0100 5510754 http://www.medworm.com/index.php?rid=5510782&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140096%26dopt%3DAbstract Authors: Nupur , Sharma S, Singh PK, Korpole S, Pinnaka AK Abstract A novel, Gram-negative, rod-shaped, obligate aerobic, non-motile bacterium, designated stain AK7T, was isolated from water sample collected from sea shore of Visakhapatnam, Andhra Pradesh, India. Colour of the colony was yellow. The predominant fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH and summed feature 3 (C16:1ω7c / iso C15:0 2-OH). Strain AK7T contained MK7 as the major respiratory quinone and phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids, two unidentified phospholipids and two unknown lipids as polar lipids. Phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that the strain AK7T is closely related to the species Fulvivirga kasyanovii (95.97... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510782 Fri, 18 Nov 2011 05:00:00 +0100 5510782 http://www.medworm.com/index.php?rid=5510775&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140156%26dopt%3DAbstract Authors: Chen WM, Lin YS, Sheu DS, Sheu SY Abstract A Gram-negative, short-rod-shaped, motile, non-spore-forming and poly-β-hydroxybutyrate-accumulating bacterial strain, designated wsw-7T, was isolated from a freshwater shrimp culture pond in Taiwan and was characterized using the polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest relatives of strain wsw-7T were Delftia lacustris 332T, Delftia tsuruhatensis T7T and Delftia acidovorans ATCC 15668T, with sequence similarities of 98.5, 98.4 and 97.9 %, respectively. Phylogenetic trees obtained with 16S rRNA gene sequences or the polyhydroxyalkanoate synthase (phaC) gene sequences reflected that the novel strain wsw-7T and these three closest relatives formed an independent ph... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510775 Fri, 18 Nov 2011 05:00:00 +0100 5510775 http://www.medworm.com/index.php?rid=5510774&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140157%26dopt%3DAbstract Authors: Ara I, Tsetseg B, Daram D, Suto M, Ando K Abstract A Gram-positive, aerobic actinomycete, strain MN08-A0118T, which produced short chains of nonmotile spores on the tips of long sporophores and formed moderate yellow brown colonies with branched substrate mycelium was studied in detail for its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0118T was grouped into the genus Herbidospora, being most closely related to Streptosporangium claviforme (98.2 %), Herbidospora osyris (98.2 %), Herbidospora daliensis (98.2 %), Herbidospora cretacea (97.9 %) and Herbidospora yilanensis (97.4 %). Chemotaxonomic data [menaquinone MK-10(H4) with minor amounts of MK-10(H6), MK-10(H2) and MK-9(H4); fatty acid with major amounts of iso-C16:0, C17:... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510774 Fri, 18 Nov 2011 05:00:00 +0100 5510774 http://www.medworm.com/index.php?rid=5510773&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140158%26dopt%3DAbstract Authors: Watthanalamloet A, Tachaapaikoon C, Lee YS, Kosugi A, Mori Y, Tanasupawat S, Kyu KL, Ratanakhanokchai K Abstract An obligately anaerobic, xylanolytic-cellulolytic bacterium, designated strain A6T, was isolated from soil in a brackish area of a coconut garden in Bangkuntien district, Thailand. The strain was Gram-stain positive and catalase-negative, endospore-forming, motile and rod-shaped with a cell size of 0.2-0.3X2.0-3.0 µm. The optimal growth of strain A6Toccurred at pH55 °C 9.5, 55 °C. Strain A6T fermented various carbohydrates, and the end products from the fermentation of cellobiose were acetate, ethanol, propionate and a small amount of butyrate. The major cellular fatty acids were iso-C14:0 3-OH, iso-C15:0, iso-C16:0 and C16:0. The cell-wall peptidoglycan con... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510773 Fri, 18 Nov 2011 05:00:00 +0100 5510773 http://www.medworm.com/index.php?rid=5510772&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140159%26dopt%3DAbstract Authors: Ulger-Toprak N, Lawson PA, Summanen P, O'Neal L, Finegold SM Abstract Two strains of previously unknown Gram-positive, anaerobic, coccus-shaped bacteria recovered from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and biochemical characteristics, demonstrated that these organisms are genotypically and phenotypically distinct and represent previously unknown sub-lines within the order Clostridiales, within the Firmicutes. Pairwise sequence analysis demonstrated that the novel organisms were 91.9% related to each other and were most closely related to members of the genus Peptoniphilus. The major long-chain fatty acids of both strains was found to be C16:0, C18:0, C18:1ω9c, C18:2 ω6,9... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510772 Fri, 18 Nov 2011 05:00:00 +0100 5510772 http://www.medworm.com/index.php?rid=5510771&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140160%26dopt%3DAbstract Authors: Du Y, Yu X, Wang G Abstract A bacterial isolate, designated strain TS3T, was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH), C16:0 and C18:1 ω7c as the major fatty acids. The G+C content was 65.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TS3T was most closely related to Massilia niastensis 5516S-1T (98.5%), Massilia consociata CCUG 58010T (97.6%), Massilia aerilata 5516S-11T (97.4%) and... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510771 Fri, 18 Nov 2011 05:00:00 +0100 5510771 http://www.medworm.com/index.php?rid=5510770&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140161%26dopt%3DAbstract Authors: Xu Y, He J, Tian XP, Li J, Yang LL, Xie Q, Tang SK, Chen YG, Zhang S, Li WJ Abstract Strains YIM M 10366T, YIM M 10378T and YIM M 10400T were isolated from marine sediments, collected from the Xisha Islands in the South China Sea. All three isolates were able to grow optimally at pH 7.0, 28°C and NaCl concentrations between 0-3 % (w/v). Comparison of 16S rRNA gene sequences showed that these strains are members of the genus Streptomyces, exhibiting moderately high 16S rRNA gene sequence similarities to 97.0-98.8% with the most closely related Streptomyces spp. Morphological characteristics, physiological characteristics, compositions of the whole-cell sugars and phospholipids are consistent with the diagnostic characteristics of the genus Streptomyces, but still allowed f... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510770 Fri, 18 Nov 2011 05:00:00 +0100 5510770 http://www.medworm.com/index.php?rid=5510769&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140162%26dopt%3DAbstract Authors: Lee S, Weon HY, Han K, Ahn TY Abstract A novel yellow-pigmented bacterial strain, designated ARSA-19T was isolated from a freshwater reservoir in Cheonan, Korea. Cells were Gram-staining-negative, rod shaped, strictly aerobic and positive for catalase and oxidase. The phylogenetic tree based on 16S rRNA gene sequences showed that strain ARSA-19T belonged to the genus Flavobacterium and was related to F. macrobrachii BCRC 17965T (96.5% sequence similarity), F. koreense KACC 14969T (96.2%), F. chungnamense KACC 14971T (96.1%), F. cheonanense KACC 14972T (96.4%), and F. aquatile JCM 20475T (95.6%). The isolate contained MK-6 as the predominant menaquinone, and iso-C15:0 (21.8%), iso-C16:0 3-OH (10.4%), iso-C15:1 G (9.3%), summed feature 3 (comprising iso-C15:0 2-OH and/or C16... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510769 Fri, 18 Nov 2011 05:00:00 +0100 5510769 http://www.medworm.com/index.php?rid=5510768&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140163%26dopt%3DAbstract Authors: Jung MY, Kim JS, Paek WK, Styrak I, Park IS, Sin Y, Paek J, Park KA, Kim H, Kim HL, Tindall BJ, Chang YH Abstract A Gram-positive, rod-shaped, endospore-forming bacterium, strain BLB-1T, was isolated from the samples of tidal flat sediment in the Yellow Sea. The 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the Bacillus rRNA group 2 and it was closely related to Bacillus massiliensis CIP 108446T (97.4%), Bacillus odysseyi ATCC PTA-4993T (96.7%), Lysinibacillus fusiformis DSM 2898T (96.2%), and Lysinibacillus boronitolerans DSM 17140T (95.9%). Sequence similarities with species in other related genera, including Caryophanon, Sporosarcina, and Solibacillus, were less than 96.1%. Chemotaxonomic data supported the affiliation of strain BLB-1T to the... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510768 Fri, 18 Nov 2011 05:00:00 +0100 5510768 http://www.medworm.com/index.php?rid=5510767&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140164%26dopt%3DAbstract Authors: Li X, Zhang L, Ding Y, Gao Y, Ruan J, Huang Y Abstract A novel actinomycete, designated strain FXJ1.034T, was isolated from acidic soil collected in Jiangxi Province, south-east China. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FXJ1.034T belonged to the genus Lentzea, with the highest sequence similarities to Lentzea kentuckyensis NRRL B-24416T (98.5%) and Lentzea albida NBRC 16102T (98.3%). Morphological and chemotaxonomic characteristics supported its assignment to the genus Lentzea. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strain from the closely related species. It is therefore proposed that FXJ1.034T represents a novel species Lentzea jiangxiens... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510767 Fri, 18 Nov 2011 05:00:00 +0100 5510767 http://www.medworm.com/index.php?rid=5510766&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140165%26dopt%3DAbstract Authors: Zhao GZ, Li J, Zhu WY, Wei DQ, Zhang JL, Xu LH, Li WJ Abstract A Gram-positive, non-motile, endophytic actinomycete, designated strain YIM 63638T, was isolated from the surface-sterilized roots of Artemisia annua L., and its taxonomic position was investigated by a polyphasic approach. The isolate grew optimally with 1-3 % (w/v) NaCl, at pH 6.0-7.0 and at 20-37°C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus Pseudonocardia, showing the highest level of sequence similarity with respect to Pseudonocardia oroxyli D10T (98.9 %), Pseudonocardia ailaonensis YIM 45505T (98.3 %) and Pseudonocardia halophobica DSM 43089T (98.0 %). The phylogenetic distances from other described species with validly published names within the genus... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510766 Fri, 18 Nov 2011 05:00:00 +0100 5510766 http://www.medworm.com/index.php?rid=5510765&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22140166%26dopt%3DAbstract Authors: Pang H, Kitahara M, Tang Z, Wang Y, Qin G, Okuma M, Cai Y Abstract Characterization and identification of the strain CW 1 (= JCM 17161) isolated from corn silage was studied. Strain CW 1 was Gram-positive, catalase-negative and homofermentative rod. This strain exhibited more than 99.6% similarities of 16S rRNA gene sequence and more than 82% DNA-DNA reassociation level with type strains of Lactobacillus (L.) kimchii, L. bobalius and L. paralimentarius. In order to clarify the taxonomic positions of these type strains, phenotypic properties, 16S rRNA gene sequencing, ribotyping and DNA-DNA relatedness experiments were examined. The three type strains displayed some different carbohydrate-fermentation patterns in L-arabinose, lactose, melibiose, melezitose, D-raffinose and ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5510765 Fri, 18 Nov 2011 05:00:00 +0100 5510765 http://www.medworm.com/index.php?rid=5417575&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081710%26dopt%3DAbstract Authors: Frischmann A, Knoll A, Hilbert F, Zasada AA, Kämpfer P, Busse HJ Abstract A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the skin of a dog, designated strain 410T, was subjected to comprehensive taxonomic characterization. Comparison of the 16S rRNA gene sequence showed highest similarities to Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, and Corynebacterium ulcerans (96.1-96.7%). The quinone system consisted predominantly of MK-8(H2) and MK-9(H2). The polar lipid profile of strain 410T contained the major compounds diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and four unidentified glycolipids. The polyamine pattern was composed of the major amines spermidine and spermi... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417575 Fri, 11 Nov 2011 05:00:00 +0100 5417575 http://www.medworm.com/index.php?rid=5417574&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081711%26dopt%3DAbstract Authors: Uchida H, Hamana K, Miyazaki M, Yoshida T, Nogi Y Abstract A Gram-negative, aerobic, motile, orange-pigmented, slightly halophilic, rod-shaped bacterium, designated strain JAMH 0132T, was isolated from a tubeworm trophosome in Kagoshima Bay, Japan, and subjected to polyphasic taxonomic characterization. The isolate grew optimally at 28-30 °C and in the presence of 2-3 % NaCl. Chemotaxonomic analysis showed that Q-10 was the predominant respiratory quinone and that C18:1 ω7c, C16:0 2-OH and C16:0 were the major fatty acids. Sphingoglycolipids, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. The DNA G +C content was 60.1 mol%. The 16S rRNA gene sequence comparison revealed that the strain JAMH 0132... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417574 Fri, 11 Nov 2011 05:00:00 +0100 5417574 http://www.medworm.com/index.php?rid=5417573&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081712%26dopt%3DAbstract Authors: Jean WD, Huang SP, Chen JS, Shieh WY Abstract Two Gram-negative strains of heterotrophic, aerobic, marine bacterial strains, designated KT1(T) and KM1(T), were isolated from seawater samples collected from the shallow coastal regions of north Taiwan. Cells grown in broth cultures were non-flagellated rods. NaCl was required for growth. Optimal growth occurred in 2-5% NaCl, 25-30 °C and pH 8. They grew aerobically and were not capable of anaerobic growth by fermenting D-glucose or other carbohydrates. Q-8 was the only isoprenoid quinone. The phospholipids of KT1(T) consisted of phosphatidylmonomethylethanolamine and phosphatidylethanolamine, whereas the phospholipids of KM1(T) consisted of phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and and an unid... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417573 Fri, 11 Nov 2011 05:00:00 +0100 5417573 http://www.medworm.com/index.php?rid=5417572&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081713%26dopt%3DAbstract Authors: Hijazin M, Prenger-Bernighoff E, Alsammara U, Alber J, Lämmler C, Kämpfer P, Glaeser SP, Busse HJ, Hassan AA, Abdulmawjood A, Zschöck M Abstract A polyphasic taxonomic study was performed on previously unidentified Arcanobacterium-like Gram-positive bacteria isolated from otitis externa of a dog.Comparative 16S rRNA gene sequencing showed that the bacteria belonged to genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum (97.2%), Arcanobacterium hippocoleae (96.5%) and Arcanobacterium phocae (96.4%). The presence of the major menaquinone MK-9 H4 supported the affiliation of this strain to genus Arcanobacterium. The polar lipid profile contains the major lipids phosphatidylcholine diphosphatidylglycerol, phosphatidylinositol-mannoside and an... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417572 Fri, 11 Nov 2011 05:00:00 +0100 5417572 http://www.medworm.com/index.php?rid=5417571&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081714%26dopt%3DAbstract Authors: López-López A, Rogel-Hernández MA, Barois I, Ortiz Ceballos AI, Martínez J, Ormeño-Orrillo E, Martinez-Romero E Abstract Two novel related Rhizobium species, Rhizobium grahamii and Rhizobium mesoamericanum were identified by a polyphasic approach using DNA-DNA hybridization, whole genome sequencing, phylogenetic and phenotypic characterization including nodulation of Leucaena leucocephala and Phaseolus vulgaris (bean). As similar bacteria were found in Los Tuxtlas rain forest and in Central America we suggest the existence of a Mesoamerican microbiological corridor. The type strain of Rhizobium grahamii sp. nov. is CCGE502T = ATCC BAA-2124T = CFN 242T = Dal4T = HAMBI 3152T and of Rhizobium mesoamericanum sp. nov. is CCGE 501T = ATCC BAA-2123T = HAMBI 3151T = CIP110148... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417571 Fri, 11 Nov 2011 05:00:00 +0100 5417571 http://www.medworm.com/index.php?rid=5417570&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081715%26dopt%3DAbstract Authors: Sheu SY, Chou JH, Bontemps C, Elliott GN, Gross E, James EK, Sprent JI, Young JP, Chen WM Abstract Four strains, JPY345T, JPY347, JPY366 and JPY581 were isolated from nitrogen-fixing nodules on the roots of two Mimosa spp. (M. cordistipula, M. misera) native to North East Brazil, and their taxonomic positions were investigated by a polyphasic approach. All four strains grew at 15-43 °C (optimum, 35 &degC), at pH 4.0-7.0 (optimum, pH 5.0) and with 0-2 % NaCl (optimum, 0 %). On the basis of 16S rRNA gene sequence analysis, a representative strain (JPY345T) showed 97.3 % sequence similarity to the closest species Burkholderia soli GP25-8T, a similarity of 97.3 % to Burkholderia caryophylli ATCC25418T and of 97.1 % to Burkholderia kururiensis KP23T. The predominant fatty ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417570 Fri, 11 Nov 2011 05:00:00 +0100 5417570 http://www.medworm.com/index.php?rid=5417569&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081716%26dopt%3DAbstract Authors: Liu YP, Wang YX, Li YX, Feng FY, Liu HR, Wang J Abstract Two isolates, MIM28T and MIM29, with an optimum growth temperature of 30-33 °C, an optimum pH for growth of 8-9 and an optimum salinity (NaCl, w/v) of 3%-4% for growth, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The strains were pink, coccus, non-motile, aerobic, alkaliphilic, halotolerant, and Gram-negative. They were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. Menaquinone MK-7 was a major respiratory quinone. Major polar lipids were phosphatidylcholine and phosphatidylethanolamine. Predominant cellular fatty acids were iso-C15:0 (13.8%-17.5%), anteiso-C15:0 (10.5%-11.2%), iso-C16:0 (9.9%-13.0%), C16:0 (4.3%-4.6%), iso-C17:0 (3.8%-5... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417569 Fri, 11 Nov 2011 05:00:00 +0100 5417569 http://www.medworm.com/index.php?rid=5417568&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081717%26dopt%3DAbstract Authors: Muramatsu Y, Takahashi M, Kamakura Y, Suzuki KI, Nakagawa Y Abstract The taxonomic position of a bacterial strain, isolated from seawater in Japan, designated AM11-6(T), was determined by using a polyphasic taxonomy approach. The strain was a strictly aerobic and Gram-staining-negative slender rod, motile by gliding. The major respiratory quinone was menaquinone-6 and the predominant cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, C14:0 and iso-G-C15:1. The polar lipid pattern indicated the presence of an unidentified phospholipid, several glycolipids and an unidentified polar lipid. The G+C content of the genomic DNA was 36.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AM11-6(T) clustered with members of the genera Wandonia and Flu... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417568 Fri, 11 Nov 2011 05:00:00 +0100 5417568 http://www.medworm.com/index.php?rid=5417567&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081718%26dopt%3DAbstract Authors: Kumar PA, Srinivas TN, Madhu S, Sravan R, Singh S, Naqvi SW, Mayilraj S, Shivaji S Abstract A novel Gram-staining-negative, rod shaped, non-motile bacterium, designated strain LW9T, was isolated from a water sample collected from Lonar Lake of Buldhana district, Maharastra, India. Colonies and broth cultures were reddish-orange due to the presence of carotenoid pigments. Strain LW9T was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. The predominant fatty acids were iso-C15:0 (31.3 %), iso-C16:0 (9.3 %), anteiso-C15:0 (7.3 %), iso-C16:1 H (6.1 %), summed feature 3 comprising C16:1 ω7c/C16:1 ω6c (5.9 %), iso-C17:1 ω9c (5.4%) and iso-C17:0 3-OH (5.0 %). Strain LW9T conta... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417567 Fri, 11 Nov 2011 05:00:00 +0100 5417567 http://www.medworm.com/index.php?rid=5417566&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081719%26dopt%3DAbstract Authors: Kämpfer P, Busse HJ, Longaric I, Rosselló-Móra R, Galatis H, Lodders N Abstract A pinkish pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain E92T), was isolated from the skin of the medical leech Hirudo medicinalis , on R2A Agar The 16S rRNA gene sequence analysis of strain E92T showed a relatively low (93.0 - 93.5%) to representatives of the genus Arcicella and 91.5% - 92.0% to members of the genus Flectobacillus. The polar lipid profile was composed of the major compounds phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified polar lipid (L1). Glycolipids were not detected. The major quinone was menquinone MK-7. Major compound in the polyamine pattern was spermidine. The predominant fatty acid... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417566 Fri, 11 Nov 2011 05:00:00 +0100 5417566 http://www.medworm.com/index.php?rid=5417565&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081720%26dopt%3DAbstract Authors: Wang QM, Boekhout T, Bai FY Abstract Five yeast strains isolated from plant leaves collected in southeast Tibet formed cream to brownish colonies and produced asymmetrical ballistoconidia and CoQ-9 as the major ubiquinone. Sequence analysis of the 26S rRNA D1/D2 domain and the internal transcribed spacer (ITS) region indicated that these strains represented two novel species of the genus Bensingtonia. Bensingtonia rectispora sp. nov. (type strain: XZ 4C5T = CGMCC 2.02635T = CBS 10710 T) and Bensingtonia bomiensis sp. nov. (type strain: XZ 33D1T = CGMCC 2.02670T=CBS 10713T) are proposed for the two new species, which are phylogenetically closely related with Bensingtonia naganoensis, Bensingtonia pseudonaganoensis and the type species of the genus Bensingtonia ciliata. ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417565 Fri, 11 Nov 2011 05:00:00 +0100 5417565 http://www.medworm.com/index.php?rid=5417564&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081721%26dopt%3DAbstract Authors: Chen MH, Sheu SY, Chen CA, Wang JT, Chen WM Abstract A bacterial strain designated GISW-4T was isolated from the reef-building coral Stylophora pistillata, collected from the seawater off coast of Southern Taiwan and was characterized using a polyphasic taxonomic approach. Strain GISW-4T was Gram-negative, aerobic, beige colored, rod-shaped, and dimorphic, with stalks (or prosthecae) and non-motile or non-stalked and motile, by monopolar flagella. The 16S rRNA gene sequence studies showed that the novel strain was closely clustered with Oceanicaulis alexandrii DSM 11625T (98.9 % 16S rRNA gene sequence similarity). Strain GISW-4T exhibited optimal growth at 35-40 °C, 1-2 % NaCl and pH 7-9. Predominant cellular fatty acids (>10 %) were C18:0, C18:1ω7c and C18:1ω7c 11-m... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417564 Fri, 11 Nov 2011 05:00:00 +0100 5417564 http://www.medworm.com/index.php?rid=5417563&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081722%26dopt%3DAbstract Authors: Yu Y, Li HR, Zeng YX, Sun K, Chen B Abstract A yellow colored, rod-shaped, Gram-negative, non-motile and aerobic bacterial strain, designated ZS1-8T, was isolated from sandy intertidal sediment sample collected from a coastal region near the Chinese Antarctic Zhongshan Station. Flexirubin-type pigments were absent. Phylogenetic analyses based on the 16S rRNA gene sequences showed that ZS1-8T represents a distinct phyletic line that reflects a novel generic status within the family Flavobacteriaceae with relatively low sequence similarities (< 93.3 %) to other validly named species. The strain required Ca2+ and K+ ions in addition to NaCl for growth. Optimal growth was observed at pH 7.5-8.0 and 17-19 °C and required the presence of 2-3% (w/v) NaCl. The dominant fatty a... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417563 Fri, 11 Nov 2011 05:00:00 +0100 5417563 http://www.medworm.com/index.php?rid=5417562&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22081723%26dopt%3DAbstract Authors: Jiang F, Li W, Xiao M, Dai J, Kan W, Chen L, Li W, Fang C, Peng F Abstract A yellow-pigmented, Gram-reaction-negative, non-motile, aerobic bacterium, designated DR4-30T, was isolated from a tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78°N). Growth occurred at 4-28°C (optimum 20-25°C) and at pH 7.0-8.0 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DR4-30T belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 95.4 and 94.7 % sequence similarity, respectively, with those of the type strains of Luteolibacter pohnpeiensis A4T-83T and Luteolibacter algae A5J-41-2T. The major respiratory quinones were MK-9 and MK-10; the predominant cellular fatty ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417562 Fri, 11 Nov 2011 05:00:00 +0100 5417562 http://www.medworm.com/index.php?rid=5417586&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058316%26dopt%3DAbstract Authors: Zhang WW, Fang MX, Tan HQ, Zhang XQ, Wu M, Zhu XF Abstract A novel Gram-negative, non-sporeforming, strictly anaerobic, heterotrophic bacterium, strain TY(T) was isolated from salty pickle wastewater. Cells were rod-shaped with comblike flagella, slightly curved and very variable in length. Optimal growth occurred at 28°C and pH 6.5. Cells were resistant to NaCl concentrate up to 50 g l(-1). Strain TY(T) produced acid from glycerin, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propanoic acid. Test for acid phosphatase and naphthol-AS-BI-phosphohydrolase activity were positive. The major fatty acids were C(14:0) (18.7%), C(15:0) (15.4%), anteiso-C(18:1) (15.2%), C(13:0) (13.3%) and summed feature 5 (11.0%). The DNA G+... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417586 Fri, 04 Nov 2011 04:00:00 +0100 5417586 http://www.medworm.com/index.php?rid=5417585&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058317%26dopt%3DAbstract Authors: Thawai C, Kudo T Abstract A novel actinomycete strain, S7F-02T, which produced primary branched hyphae and fragmented into V-shape or Y-shaped bacillary cell, was isolated from marine sediment collected in Andaman sea, Trang province, Thailand. Lysine was found to be a diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars of strain S7F-02T, were ribose, arabinose and glucose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H4). The predominant cellular fatty acids were anteiso-C15:0 and iso-C15:0. The G+C content of the genomic DNA was 70.6 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain S7F-02T should be classified in the... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417585 Fri, 04 Nov 2011 04:00:00 +0100 5417585 http://www.medworm.com/index.php?rid=5417584&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058318%26dopt%3DAbstract Authors: Vandamme P, De Brandt E, Houf K, De Baere T Abstract GyrB sequence analysis, (GTG)5-primed PCR fingerprinting and biochemical characteristics present in the Biolog GEN III microtest system were used to differentiate an unnamed Kerstersia species from Kerstersia gyiorum, the type and only named species in this genus. The inability to oxidize D-galacturonic and D-glucuronic acid and the capacity to oxidize D-serine, along with the gyrB sequences and (GTG)5-PCR fingerprints readily differentiate the unnamed taxon from the type species. Therefore, we propose to formally classify this unnamed taxon as Kersteria similis sp. nov. with strain LMG 5890T (=CCUG 46999T), isolated from a leg wound in the USA in 1983, as the type strain. PMID: 22058318 [PubMed - as supplied by publ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417584 Fri, 04 Nov 2011 04:00:00 +0100 5417584 http://www.medworm.com/index.php?rid=5417583&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058319%26dopt%3DAbstract Authors: Raichand R, Pareek S, Singh NK, Mayilraj S Abstract A Gram positive, motile, short rod shaped, orange pigmented bacterium strain IMTB-3094T, isolated from water sample, collected from Tikkar Tal Lake, Haryana and was subjected to a detailed polyphasic taxonomic study. Strain IMTB-3094T, matched with most of the phenotypic and chemotaxonomic properties of the genus Exiguobacterium and represented novel species. According to 16S rRNA gene sequence analysis, strain IMTB-3094T belonged to the genus Exiguobacterium. The strain IMTB-3094T exhibited highest 16S rRNA gene sequence similarity with Exiguobacterium mexicanum MTCC 7759T (99.5%) followed by Exiguobacterium aurantiacum MTCC 6414T (99.1%), Exiguobacterium aestuarii MTCC 7750T (98.0%), Exiguobacterium profundum MTCC 10851... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417583 Fri, 04 Nov 2011 04:00:00 +0100 5417583 http://www.medworm.com/index.php?rid=5417582&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058320%26dopt%3DAbstract Authors: Minegishi H, Echigo A, Shimane Y, Kamekura M, Tanasupawat S, Visessanguan W, Usami R Abstract Halobacterium piscisalsi was proposed by Yachai et al. (2008), with a single strain HPC1-2T (=BCC 24372T =JCM 14661T =PCU 302T) isolated from fermented fish (pla-ra) in Thailand. According to the orignal paper, it was closely related to Halobacterium salinarum by means of the 16S rRNA gene sequence comparison: even so, it could be differentiated by the low DNA-DNA relatedness and different biochemical profiles as the independent species. The re-analyses of the 16S rRNA gene sequences and the DNA-DNA relatedness among Halobacterium piscisalsi JCM 14661T, Halobacterium salinarum JCM 8978T as well as strains R1 and NRC-1 revealed that they all had exactly the same 16S rRNA gene seque... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417582 Fri, 04 Nov 2011 04:00:00 +0100 5417582 http://www.medworm.com/index.php?rid=5417581&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058321%26dopt%3DAbstract Authors: Zhang YJ, Tang SK, Shi R, Klenk HP, Chen C, Yang LL, Zhou Y, Li WJ Abstract A Gram-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161T, was isolated from brine of a salt well in Yunnan province of China, and subjected to a polyphasic taxonomic study. Cells of strain YIM 95161T were short rods, approximately 0.9-1.4 µm in length and 0.4-0.6 µm in width. Strain YIM 95161T grew at 15-40 °C (optimum 25-30 °C), 6-29 % NaCl (optimum 14-19 %) and pH 5.0-8.0 (optimum pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were Summed feature 8 (C18:1ω6c and/or C18:1ω7c) and C14:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown p... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417581 Fri, 04 Nov 2011 04:00:00 +0100 5417581 http://www.medworm.com/index.php?rid=5417580&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058322%26dopt%3DAbstract Authors: Niemi RM, Ollinkangas T, Paulin L, Svec P, Vandamme P, Karkman A, Kosina M, Lindström K Abstract A significant number of Enterococcus strains from pristine waters formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with following characteristics: ovoid Gram-positive nonsporeforming cells as single, pairs or chains, facultatively anaerobic, catalase-negative, growth in broth containing 6.5% NaCl or at 45°C weak or absent, variable production of D antigen, tolerate 60°C for 30 min, 40% bile and tellurite, hydrolyse aesculin strongly and gelatin weakly, produce no acid from hippurate and do not reduce it, weak growth at 10 °C, strong reaction for Voges-Proskau... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417580 Fri, 04 Nov 2011 04:00:00 +0100 5417580 http://www.medworm.com/index.php?rid=5417579&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058323%26dopt%3DAbstract Authors: Jung YT, Kim JH, Kang SJ, Oh TK, Yoon JH Abstract A Gram-staining-negative, non-flagellated, non-gliding and pleomorphic bacterial strain, designated DPG-25T, was isolated from seawater in a seaweed farm of the South Sea in Korea, and its taxonomic position was investigated by using a polyphasic study. Strain DPG-25T grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Flexirubin-type pigments were not produced. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DPG-25T formed a cluster with the type strains of Actibacter sediminis, Aestuariicola saemankumensis and Lutimonas vermicola. Strain DPG-25T exhibited 16S rRNA gene sequence similarity values of 95.3, 93.1 and 93.6 % to the type strains of A. sediminis, A. saemanku... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417579 Fri, 04 Nov 2011 04:00:00 +0100 5417579 http://www.medworm.com/index.php?rid=5417578&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058324%26dopt%3DAbstract Authors: Zhao CT, Wang ET, Zhang YM, Chen WF, Sui XH, Chen WX, Liu HC, Zhang XX Abstract Four rhizobial strains representing a previously defined novel group in the genus Mesorhizobium associated with Astragalus species in China were further characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus Mesorhizobium with Mesorhizobium plurifarium LMG 11892T as the closest neighbour sharing a sequence similarity of 99.8%. Polyphasic taxonomic methods including comparative sequence analysis of the atpD, recA, glnII, rpoB, nodC and nifH genes, SDS-PAGE of whole-cell soluble proteins, DNA-DNA hybridization, fatty acid profiles and a series of phenotypic and physiological tests allowed to differ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417578 Fri, 04 Nov 2011 04:00:00 +0100 5417578 http://www.medworm.com/index.php?rid=5417577&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058325%26dopt%3DAbstract Authors: Männistö M, Rawat S, Starovoytov V, Haggblom MM Abstract Four aerobic bacteria, designated MP5ACTX2T, MP5ACTX8T, MP5ACTX9T and S6CTX5AT were isolated from tundra soil of north-western Finland (69°01'N, 20°50'E). Cells of all strains were Gram-negative, non-motile rods. Phylogenetic analysis indicated that they belong to the genus Granulicella in subdivision 1 of the Acidobacteria. The 16S rRNA gene sequence similarities among the strains and previously described Granulicella aggregans, Granulicella paludicola, Granulicella pectinivorans and Granulicella rosea Type strains ranged from 94 to 99%. Analysis of the RNA polymerase beta subunit (rpoB) gene similarities indicated that the tundra soil isolates represent novel Granulicella species, with rpoB gene homologies <... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417577 Fri, 04 Nov 2011 04:00:00 +0100 5417577 http://www.medworm.com/index.php?rid=5417576&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22058326%26dopt%3DAbstract Authors: Krishnamurthi S, Bhattacharya A, Schumann P, Dastager SG, Tang SK, Li WJ, Chakrabarti T Abstract A Gram-positive, non-endospore forming bacterium designated as strain SK 18T was isolated from surface soil of a landfill by dilution plating on tryptic soy broth agar (TSBA). Preliminary characterization of strain SK 18T using biochemical tests, FAME analysis and partial 16S rRNA gene sequencing placed it within the genus Microbacterium. Analysis of the cell wall indicated that the peptidoglycan was of the B-type of cross-linkage containing amino acids lysine and ornithine with muramic acid in the N-glycolyl form. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The major fatty acids of the cell me... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5417576 Fri, 04 Nov 2011 04:00:00 +0100 5417576 http://www.medworm.com/index.php?rid=5378342&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053326%26dopt%3DAbstract Authors: Abstract The purpose of this announcement is to effect the valid publication of the following effectively published new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof, or an electronic copy of the published paper, to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries. ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378342 Tue, 01 Nov 2011 04:00:00 +0100 5378342 http://www.medworm.com/index.php?rid=5378341&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053327%26dopt%3DAbstract Authors: Abstract This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission. PMID: 22053327 [PubMed - in process] (Source: Internationa... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378341 Tue, 01 Nov 2011 04:00:00 +0100 5378341 http://www.medworm.com/index.php?rid=5378340&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053328%26dopt%3DAbstract Authors: Garrity GM, Labeda DP, Oren A PMID: 22053328 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378340 Tue, 01 Nov 2011 04:00:00 +0100 5378340 http://www.medworm.com/index.php?rid=5378339&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053329%26dopt%3DAbstract Authors: Labeda DP, Oren A PMID: 22053329 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378339 Tue, 01 Nov 2011 04:00:00 +0100 5378339 http://www.medworm.com/index.php?rid=5378338&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053330%26dopt%3DAbstract Authors: Oren A, Ventosa A PMID: 22053330 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378338 Tue, 01 Nov 2011 04:00:00 +0100 5378338 http://www.medworm.com/index.php?rid=5378337&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053331%26dopt%3DAbstract Authors: Arahal DR, Ventosa A PMID: 22053331 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378337 Tue, 01 Nov 2011 04:00:00 +0100 5378337 http://www.medworm.com/index.php?rid=5378336&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22053332%26dopt%3DAbstract Authors: Arahal DR, Oren A, Ventosa A PMID: 22053332 [PubMed - in process] (Source: International Journal of Systematic and Evolutionary Microbiology) International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378336 Tue, 01 Nov 2011 04:00:00 +0100 5378336 http://www.medworm.com/index.php?rid=5378354&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22038998%26dopt%3DAbstract Authors: Tazato N, Nishijima M, Handa Y, Kigawa R, Sano C, Sugiyama J Abstract Six Gram-negative, rod-shaped, non-spore-forming bacterial strains were isolated from small holes on plaster walls of the stone chamber interior of the Kitora Tumulus in Asuka village, Nara Prefecture, Japan. These were investigated by means of a polyphasic approach. All the isolates were strictly aerobic and motile by peritrichous flagella. Phylogenetic trees generated using 16S rRNA gene sequences detected the existence of two novel lineages (comprising five isolates and one isolate) within the genus Gluconacetobacter. The isolates were characterized by having Q-10 as the major ubiquinone system and C18:1ω7c (58.7-63.1 %) as the predominant fatty acid. Our DNA-DNA hybridization experiments endorsed th... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378354 Fri, 28 Oct 2011 04:00:00 +0100 5378354 http://www.medworm.com/index.php?rid=5378350&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22038999%26dopt%3DAbstract Authors: Pugin B, Blamey JM, Baxter BK, Wiegel J Abstract Novel strains of facultatively aerobic, moderately alkaliphilic and facultatively halophilic bacteria were isolated from a sediment sample taken from the Southern Arm of Great Salt Lake, Utah. Cells of strain JW/BP-GSL-QDT (and related strains JW/BP-GSL-RA and JW/BP-GSL-WB) were rod-shaped, spore forming, motile bacteria with a variable Gram-staining. Strain JW/BP-GSL-QDT grew under aerobic conditions between 14.5 and 47 °C (optimum at 39 °C), in the pH37 °C range 6.5-10.3 (optimum pH37 °C 8.0), and between 0.1-4.5 M Na+ (optimum at 0.9 M Na+). No growth was observed in the absence of supplemented Na+. Strain JW/BP-GSL-QDT utilized L-arabinose, D-fructose, D-galactose, D-glucose, inulin, lactose, maltose, mannitol, D-man... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378350 Fri, 28 Oct 2011 04:00:00 +0100 5378350 http://www.medworm.com/index.php?rid=5378349&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039000%26dopt%3DAbstract Authors: Zhang DC, Busse HJ, Wieser C, Liu HC, Zhou YG, Schinner F, Margesin R Abstract A Gram-negative, facultative anaerobic, psychrophilic, motile and rod-shaped bacterial strain, BZ59T, was isolated from hydrocarbon-contaminated soil and was subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ59Twas related to the genus Candidimonas and had highest 16S rRNA gene sequence pairwise similarity with Candidimonas nitroreducens SC-089T (97.7 %) and Candidimonas humi SC-092T (97.6 %). Strain BZ59T had Q-8 as the ubiquinone and C16:0, C17:0 cyclo and summed feature 3 (C16:1ω7c and/or iso-C15:02-OH) were the major fatty acids. The polar lipid profile contained the major compounds phosphatidylethanolamine, phosph... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378349 Fri, 28 Oct 2011 04:00:00 +0100 5378349 http://www.medworm.com/index.php?rid=5378348&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039001%26dopt%3DAbstract Authors: Gao ZM, Xiao J, Wang XN, Ruan LW, Chen XL, Zhang YZ Abstract A taxonomic study was carried out on a cellulase-producing bacterium G21T isolated from mangrove soil in Xiamen, Fujian province of China. Cells were Gram-negative, slightly curved rods and motile with a single polar flagellum. The strain grew at 15-40°C and in 0.5-10% NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G21T belonged to genus Vibrio and formed a clade with V. furnissii ATCC 350116T (sequence similarity, 97.4%), V. fluvialis LMG 7894T (97.1%) and V. ponticus CECT 5869T (96.1%). However, multilocus sequence analysis (rpoA, recA, mreB, gapA, gyrB and pyrH) and DNA-DNA hybridization experiments indicated that it was distinct from the closest Vibrio species. Additi... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378348 Fri, 28 Oct 2011 04:00:00 +0100 5378348 http://www.medworm.com/index.php?rid=5378347&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039002%26dopt%3DAbstract Authors: Sorokin DY, Tourova TP, Panteleeva AN, Muyzer G Abstract Two novel types of heterotrophic sulfate-reducing bacteria (SRB) were isolated from anoxic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia) using propionate as energy and carbon source. Strain APT2T was enriched and isolated with thiosulfate and strains APS1T andASS1 with sulfate as electron acceptor. APT2T was a short rod motile with a single subpolar flagellum, while APS1T/ASS1 were lemon-shaped oval rods motile with a single polar flagellum and also producing multiple thin flagella-like filaments. Strain APT2T grew by complete oxidation of C3-C8 fatty acids with thiosulfate and sulfate as electron acceptors, while strains APS1T and ASS1 were much less versatile utilizing only propionate and py... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378347 Fri, 28 Oct 2011 04:00:00 +0100 5378347 http://www.medworm.com/index.php?rid=5378346&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039003%26dopt%3DAbstract Authors: Takebe F, Hirota K, Nodasaka Y, Yumoto I Abstract A heterotrophic nitrifying bacterium, designated as strain DA2(T), was isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. Cells of strain DA2(T) were Gram-positive, facultatively anaerobic, motile by means of peritrichous flagella and sporulating rods, and grew in the pH range of 5-8. Its major isoprenoid quinone was menaquinone-7 (MK-7), and its cellular fatty acid profile mainly consisted of iso-C15:0 (18.6%) and anteiso-C15:0 (69.1%). The DNA G+C content was 54.1 mol%. The 16S rRNA phylogeny suggested that strain DA2T is a member of the genus Brevibacillus, with Brevibacillus choshinensis (99.7%), B. formosus (99.4%), B. brevis (99.4%), B. agri(99.0%), B. reuszeri (98.8%), ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378346 Fri, 28 Oct 2011 04:00:00 +0100 5378346 http://www.medworm.com/index.php?rid=5378345&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039004%26dopt%3DAbstract Authors: Shivaji S, Reddy PV, Rao SS, Begum Z, Manasa P, Srinivas TN Abstract A novel Gram-stain-negative, horseshoe shaped, non-motile bacterium, designated strain M12-11BT, was isolated from a marine sediment sample collected at a depth of 200 m from Kongsfjorden, Svalbard. The colony color was orangish-red due to the presence of carotenoids. The fatty acids were dominated by branched and unsaturated fatty acids (90.8%), with a high abundance of iso-C15 : 0 (14.9%), anteiso-C15 : 0 (11.4), iso-C15 : 1 G (13.1), C15 : 1 ω6c (5.4), C17 : 1 ω6c (6.7), Summed Feature 3 (9.3) and Summed Feature 9 (5.9%). Strain M12-11BT contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylcholine (PC), phosphatidylethanolamine (PE), one unidentified aminolipid (... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378345 Fri, 28 Oct 2011 04:00:00 +0100 5378345 http://www.medworm.com/index.php?rid=5378344&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039005%26dopt%3DAbstract Authors: Anand S, Bala K, Saxena A, Schumann P, Lal R Abstract A Gram-positive, heterotrophic, aerobic, non-motile, non-endospore forming, rod-shaped and yellow colored bacterium N5T was isolated from a soil sample collected from the industrial waste site in Noida at the outskirts of Delhi, India. Strain N5T showed highest 16S rRNA gene similarity to Microbacterium indicum LMG 23459T (97.59 %) and Microbacterium gubbeenense LMG 19263T (97.18 %). The 16S rRNA gene sequence similarity between strain N5T and the validly published name of the species of genus Microbacterium ranged from 94.0 % to 97.6 %. However, DNA-DNA hybridization studies showed a low reassociation value of 11% DNA-DNA relatedness between N5T and its nearest relatives. The DNA G + C content was 68 mol %. The chemota... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378344 Fri, 28 Oct 2011 04:00:00 +0100 5378344 http://www.medworm.com/index.php?rid=5378343&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22039006%26dopt%3DAbstract Authors: Jiang F, Cao SJ, Li ZH, Fan H, Li HF, Liu WJ, Yuan HL Abstract An orange-pigmented, Gram-positive, rod-shaped, non-spore-forming, halophilic, alkali-tolerant bacterium designated halo-2T was isolated from the sediment of Xiarinaoer soda lake located at the Inner Mongolia Autonomous Region, China. Strain halo-2T grew in the complex medium with presence of 3~30% (w/v) NaCl and at pH 5-10. The cell wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of the isolate were anteiso-C15: 0 (43.6%), anteiso-C17: 0 (14.8%) and iso-C15:0 (6.8%). Polar lipids consisted mainly of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PME), and an orange pigment was also detected. The genomic D... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378343 Fri, 28 Oct 2011 04:00:00 +0100 5378343 http://www.medworm.com/index.php?rid=5378372&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021574%26dopt%3DAbstract Authors: Bibi F, Chung EJ, Khan A, Jeon CO, Chung YR Abstract During a study of endophytic bacteria from coastal dune plants, a bacterial strain, designated YC6881T, was isolated from the root of Rosa rugosa collected from the coastal dune areas of Namhae Island. The bacterium was found to be Gram-negative, motile, halophilic and heterotrophic with a polar single flagellum. Strain YC6881T exhibited growth at temperature 4-37 °C (optimum at 28-32 °C), pH 6.0-9.0 (optimum at pH 7.0-8.0) and was able to grow at NaCl concentrations in the range of 0-7.5 % (w/v) with optimum growth at 4-5 % NaCl. Strain YC6881T was catalase and oxidase positive and negative for nitrate reduction. According to the 16S rRNA genes phylogenetic analysis, strain YC6881T belonged to the genus Rhizobium and ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378372 Fri, 21 Oct 2011 04:00:00 +0100 5378372 http://www.medworm.com/index.php?rid=5378370&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021575%26dopt%3DAbstract Authors: Kumar PA, Srinivas TN, Manasa P, Madhu S, Shivaji S Abstract A novel Gram-negative, oval to rod shaped, motile bacterium, strain AMV1T, was isolated from a soil sample collected from a mud volcano, Baratang Island, Andamans, India. The predominant fatty acids were C16:0 (5.7%), C18:1 ω7c (78.6 %) and C19:0 cyclo ω8c (6.3%). Strain AMV1T contained ubiquinone 10 (Q-10) as the major respiratory quinone and minor quantities of ubiquinone 9 (Q-9). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified lipids, one unidentified phospholipids and one unidentified aminolipid. The 16S rRNA gene sequence analysis indicated that Tepidamorphus gemmatus, Bauldia consociata, Afifella pfennigii and Amorphus coralli, four ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378370 Fri, 21 Oct 2011 04:00:00 +0100 5378370 http://www.medworm.com/index.php?rid=5378368&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021576%26dopt%3DAbstract Authors: Shi Z, Luo G, Wang G Abstract A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain T26T, was isolated from subsurface soil of Tianjin coal mine, China. Colonies were yellow-white, convex, circular, smooth, non-transparent on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T26T was closely related to Cellulomonas species and an Actinotalea species with 96.8-94.7% and 96.7% sequence similarities, respectively. The peptidoglycan type of strain T26T was A4β, containing L-ornithine-D-glutamic acid as a interpeptide bridge. The cell-wall sugars were rhamnose, galactose, xylose and inositol. The major fatty acids (> 10%) were anteiso-C15:0 (33.6%), anteiso-C15:1 A (22.1%), C16:0 (14.4%), and C14:0 (12.1%). The predom... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378368 Fri, 21 Oct 2011 04:00:00 +0100 5378368 http://www.medworm.com/index.php?rid=5378366&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021577%26dopt%3DAbstract Authors: Kim JJ, Kanaya E, Weon HY, Koga Y, Takano K, Dunfield PF, Kwon SW, Kanaya S Abstract A strictly aerobic, Gram-staining-negative, yellow-pigmented, non-spore forming, rod-shaped bacterium, designated strain 15C3(T), was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9-33 °C (optimum, 25 °C) and pH 5.6-7.9 (optimum, pH 6.1-7.0). No growth occurred in the presence of > 2 % (w/v) NaCl. Strain 15C3(T) reduced nitrate to nitrogen and showed catalase activity but no oxidase activity. The predominant fatty acids were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (comprising C(16:1) ω7c and/or iso-C(15:0) 2-OH), and strain 15C3(T) contained phosphatidylethanolamine as the major polar lipid. The major respiratory quinone... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378366 Fri, 21 Oct 2011 04:00:00 +0100 5378366 http://www.medworm.com/index.php?rid=5378364&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021578%26dopt%3DAbstract Authors: Jung YT, Park S, Oh TK, Yoon JH Abstract A Gram-negative, non-motile, ovoid to rod shaped bacterial strain, designated HWDM-33T, was isolated from seawater of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain HWDM-33T grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HWDM-33T clustered with Erythrobacter gangjinensis K7-2T, with which it exhibited 96.9 % sequence similarity. Strain HWDM-33T exhibited 94.2-95.8 % 16S rRNA gene sequence similarity to the type strains of the other Erythrobacter species. Strain HWDM-33T contained Q-10 as the predominant ubiquinone and C18:1ω7c, C17:1 ω... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378364 Fri, 21 Oct 2011 04:00:00 +0100 5378364 http://www.medworm.com/index.php?rid=5378362&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021579%26dopt%3DAbstract Authors: Vogelmann SA, Chaves S, Hertel C Abstract A novel anamorphic yeast, strain LTH 6662T, was isolated from cassava sourdough. The isolate supposedly originated from cassava flour or was a contaminant thereof. Sequencing of the D1/D2 domain of the 26S rRNA gene indicated that strain LTH 6662T represents a new species. Its closest relatives were the species from the Cryptococcus humicola complex. The novel strain had several physiological characteristics that differed from those of related species: the ability to assimilate raffinose and cadaverine, the inability to assimilate soluble starch, xylitol, galactitol, butane-2,3-diol, sodium nitrite and lysine, the ability to grow without vitamins and at 42 °C, and the inability to produce starch-like substances. Its major ubiquino... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378362 Fri, 21 Oct 2011 04:00:00 +0100 5378362 http://www.medworm.com/index.php?rid=5378360&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021580%26dopt%3DAbstract Authors: Volokhov DV, Amselle M, Beck BJ, Popham DL, Whittaker P, Wang H, Kerrigan E, Chizhikov VE Abstract Three strains of lactic acid bacteria (LAB) were isolated from feces of apparently healthy wild Canada geese (Branta canadensis) in 2010 using the cultivation of fecal LAB on Rogosa SL agar under aerobic conditions. These three isolates were found to share 99.9% sequence similarity of their 16S rRNA, the 16S-23S intergenic transcribed spacer region (ITS), partial 23S rRNA, rpoB, rpoC, rpoA, and pheS gene sequences. However, at the same time these strains exhibited lower levels of sequence similarity of these genetic targets to all known LAB and the phylogenetically closest species to the geese strains were Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus,... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378360 Fri, 21 Oct 2011 04:00:00 +0100 5378360 http://www.medworm.com/index.php?rid=5378358&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021581%26dopt%3DAbstract Authors: Jung YT, Park S, Lee JS, Oh TK, Yoon JH Abstract A Gram-staining-negative, non-motile, ovoid to rod-shaped bacterial strain, designated HDW-32T, was isolated from seawater of the Yellow Sea, Korea. Strain HDW-32T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. Strain HDW-32T exhibited highest 16S rRNA gene sequence similarity value (95.1 %) to Nitratireductor basaltis J3T, and sequence similarity values of 94.8 % to Ahrensia kielensis IAM 12618T and less than 94.2 % to other species used in the phylogenetic analysis. In the neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences, strain HDW-32T clustered with Ahrensia kielensis IAM 12618T. Strain HDW-32T contained Q-10 as the predomina... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378358 Fri, 21 Oct 2011 04:00:00 +0100 5378358 http://www.medworm.com/index.php?rid=5378357&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021582%26dopt%3DAbstract Authors: Jung YT, Oh TK, Yoon JH Abstract A Gram-negative, motile, rod-shaped bacterial strain, designated HDW-15T, was isolated from seawater of the Yellow Sea, Korea, and subjected to a polyphasic taxonomic study. Strain HDW-15T grew optimally at pH 7.0-8.0, at 25°C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HDW-15T fell within the clade comprising Marinomonas species, joining the type strain of Marinomonas arctica, with which it exhibited highest 16S rRNA gene sequence similarity (97.7 %). The 16S rRNA gene sequence similarity values between strain HDW-15T and the type strains of the other Marinomonas species were in the range of 93.7-97.2 %. Mean DNA-DNA relatedness value between strain HDW-15T and the typ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378357 Fri, 21 Oct 2011 04:00:00 +0100 5378357 http://www.medworm.com/index.php?rid=5378356&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021583%26dopt%3DAbstract Authors: Hong SW, Park JM, Kim SJ, Chung KS Abstract A novel swarming and moderately halotolerant bacterium, designated as strain A1-2, was isolated from the intestinal tract of earthworm (Eisenia fetida L.). Cells of the isolate were rod shaped and endospore forming. Strain A1-2 was facultatively anaerobic, catalase-positive, oxidase-negative, and motile with peritrichous flagella. The optimal growth conditions of the strain were a temperature of 30 °C and a pH of 7.0. Its growth was inhibited by 10% (w/v) NaCl concentration. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain A1-2 belongs to the genus Bacillus. The species most closely related to strain A1-2 were Bacillus drentensis LMG 21831T, Bacillus horneckiae PT-45T, Bacillus niacin BAC 1015T, Bacillus in... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378356 Fri, 21 Oct 2011 04:00:00 +0100 5378356 http://www.medworm.com/index.php?rid=5378355&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22021584%26dopt%3DAbstract Authors: Sazak A, Camas M, Spröer C, Klenk HP, Sahin N Abstract A novel actinobacterium strain, A8036T, isolated from soil, was determined using polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generate spiral chains spores with irregular-surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9 (H6) and MK-9 (H4). The phospholipids were diphosphatidyl glycerol, phosphatidyl inositol and phosphatidyl inositol mannosides. The major cellular fatty acids are iso-C16:0, cis9-C17:1, C16:0, C15:0 and 10-methyl C17:0. Based on the analysis of the 16S rRNA gene sequence, the closest phylogenetic neighbours of strain A8036T were A.... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378355 Fri, 21 Oct 2011 04:00:00 +0100 5378355 http://www.medworm.com/index.php?rid=5378412&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22003033%26dopt%3DAbstract Authors: Mezghani M, Alazard D, Karray F, Cayol JL, Joseph M, Postec A, Fardeau ML, Tholozan JL, Sayadi S Abstract An obligatory anaerobic, moderately halophilic bacterium, strain CEJFG43T, was isolated from a sediment sample taken below a salt crust of the evaporitic hypersaline lake El Jerid, in South of Tunisia. Cells were Gram-negative, non-sporulated, motile, short rods. Strain CEJFG43T was halophilic growing in media with up to 30% NaCl (w/v), with the optimum at 15%. It was a mesophile and grew at temperatures from 25 to 60°C with an optimum at 45°C. It grew within a pH range from 6.0 to 9.6, and an optimum at pH 8.3. The microorganism fermented glucose, fructose, ribose, raffinose, galactose, mannose, sucrose, maltose, xylose, mannitol, pyruvate, glycerol, and trimethylam... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378412 Fri, 14 Oct 2011 04:00:00 +0100 5378412 http://www.medworm.com/index.php?rid=5378411&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22003034%26dopt%3DAbstract Authors: Zhou Z, Jiang F, Wang S, Peng F, Dai J, Li W, Fang C Abstract A Gram-staining-negative, short rod-shaped bacterial strain motile by gliding, designated A12T, was isolated from tundra soil collected from Ny-Ålesund, in the Arctic region of Norway. The temperature, NaCl and pH ranges for growth were 4-25°C (optimum, 18°C), 0%-2% (optimum, 0%) and pH6-9 (optimum, pH7), respectively. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the Arctic isolate belonged to the genus Pedobacter and showed the highest sequence similarity (94. 4%) to Pedobacter daechungensis KCTC 12637T. The DNA G+C content (38.3 mol%), the polar lipid profile, the presence of sphingolipid, of MK-7 as the only respiratory quinone and of summed feature 3 (comprising C16:1ω7c and/or ... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378411 Fri, 14 Oct 2011 04:00:00 +0100 5378411 http://www.medworm.com/index.php?rid=5378410&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22003035%26dopt%3DAbstract Authors: Tarhriz V, Nematzadeh G, Zununi Vahed S, Hejazi MA, Hejazi MS Abstract A novel Gram-negative, aerobic, nonmotile and rod-shaped bacterium was isolated from Qurugol Lake located in Azarbayjan region; a mountain region at northwest of Iran. Growth occurred at pH 6-10 (optimum, pH 7 plusmn 0.5) and at 10-40 Centigrade (optimum, 30 Centigrade). Strain RCRI4T was able to grow in the absence and presence of NaCl to 3% (W/V). The major fatty acids were C17:0, C16:1 ω7c / C15 iso 3-OH, C17:1 ω8c, and C16:0. The G+C content of genomic DNA was 45.3 mol%. Based on the 16S rRNA and gyrase B (gyrB) gene sequences, phylogenetic analyses indicated that strain RCRI4T associated with the genus Alishewanella and closely related type strains include Alishewanella agri BLO6T (97.8%), Alishe... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378410 Fri, 14 Oct 2011 04:00:00 +0100 5378410 http://www.medworm.com/index.php?rid=5378409&cid=s_37901_77_f&fid=37901&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22003036%26dopt%3DAbstract Authors: Park SC, Choe HN, Baik KS, Seong CN Abstract An orange-coloured and rod shaped gliding bacterium, designated strain PSC33T was isolated from the gut microflora of mussel collected from the South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, catalase- and oxidase-positive. The major fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G, C15:0 3-OH and iso-C17:1ω9c. The DNA G+C content was 37.9 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW382T forms an evolutionary lineage within the radiation enclosing the members of the genus Aquimarina with A. macrocephali JAMB N27T as its nearest neighbor (96.3 % sequence similarity). A number of phenotypic characteristics distinguished strain PSC33T from described me... International Journal of Systematic and Evolutionary Microbiology journals http://www.medworm.com/rss/comments.php?id=5378409 Fri, 14 Oct 2011 04:00:00 +0100 5378409