Jornal Brasileiro de Patologia e Medicina Laboratorial via MedWorm.com

Comparison of three different protocols for extracting RNA from formalin-fixed paraffin-embedded tissues

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: All the protocols produced sufficient and adequate amounts of total RNA. However, only protocols using Arcturus and Ambion kits generated suitable RNA for PCR amplification. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Classification of haematopoietic and lymphoid tumors: WHO, standardization of nomenclature in Portuguese, 4th edition

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

INTRODUÇÃO: A classificação da Organização Mundial da Saúde (OMS) para os tumores do tecido hematopoético e linfoide (4ª edição, 2008) representa uma revisão atualização da 3ª edição publicada em 2001. A tradução da nomenclatura utilizada para identificar as entidades descritas deve ser clara, precisa e uniforme no sentido de reproduzir de forma correta as diversas entidades clinicopatológicas para clínicos, patologistas e pesquisadores envolvidos na área da onco-hematopatologia. OBJETIVO: Os autores apresentam uma proposta de atualização e padronização terminológica em língua portuguesa, com base na OMS/2008INTRODUCTION: The World Health Organization (WHO) classification of hematopoietic and lymphoid tissue (4th edition, 2008) tumors constitutes an updated revi...

Immunohistochemistry in bone marrow biopsy: an important complementary tool for morphology

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

Por razões técnicas e históricas, a utilização da imuno-histoquímica (IHQ) em biópsias de medula óssea (BMO) levou algum tempo a ocupar espaço na avaliação diagnóstica desse tipo de material. Entretanto, esse cenário vem se modificando graças ao crescimento exponencial do número de anticorpos disponíveis para a utilização em material incluído em parafina, além do aperfeiçoamento das técnicas de recuperação antigênica e descalcificação do material. Este texto tem a finalidade de auxiliar o patologista na seleção/ interpretação de painéis de anticorpos utilizados nos laboratórios de rotina, de acordo com a experiência do autor, assim como de enumerar referências da literatura de grande utilidade para a prática diagnóstica.Due to historical and technical r...

Histomorphometry, apoptosis and cell proliferation in cervical intraepithelial neoplasia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: These data indicate that the pre-neoplastic process is dynamic and is concomitant with apoptosis and mitosis. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Influence of vitamin D on osteoclastic activity in a bone organ culture model

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: VitD increases osteoclastic activity in vitro in a dose-dependent effect. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

H1N1: overview and perspectives

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

O vírus influenza de origem suína, A/California/04/2009 (H1N1), foi inicialmente detectado no México e determinou a pandemia de influenza de 2009. Em agosto de 2010, a Organização Mundial da Saúde (OMS) declarou o início da fase pós-pandêmica. As características dessa última pandemia foram marcadamente diferentes das anteriores. O vírus emergiu de rearranjos genéticos originários em hospedeiro mamífero não humano, demonstrou transmissibilidade interespécies e afetou a população humana de forma diferente dos vírus pandêmicos anteriores (1918, 1957 e 1968) com maior morbidade e mortalidade em crianças e adultos jovens. Atualmente, o vírus apresenta padrão sazonal da mesma forma que o influenza A H3N2 e o influenza B, mantendo, até o momento, o mesmo perfil de patogen...

Diagnosis of immunophenotyping of chronic lymphoproliferative syndromes by flow cytometry at HEMOPA blood center

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: Based on the antibody panel recommended in this investigation, the immunophenotyping method by flow cytometry associated with morphological characterization of peripheral blood samples is a reliable, rapid, feasible, and non-invasive procedure for the diagnosis of chronic lymphoproliferative syndromes. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Case report of hairy cell leukemia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

The objective of this study was to report the case of a male patient, tractor operator, diagnosed with HCL, pancytopenia, cutaneous lesions, without splenomegaly and positive markers for B-cell lymphocytes (CD19, CD20, CD22, CD79b, CD23, Lambda, immunoglobulin M [IgM], CD25 and CD103). Although HCL is a rare disease, late diagnosis may ultimately lead to severe complications and patient's death. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Understanding commutability: important quality requirement for clinical laboratories

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: Through a practical, simple, and internationally standardized procedure, this study showed that test results from the evaluated instruments were equivalent, which allows their use in patient monitoring. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Frequence and resistance profile of Klebsiella spp. isolates in a university hospital in Natal/RN during a ten-year period

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

CONCLUSION: The increase in resistance to antibiotics reinforces the importance of continuous monitoring, which elucidates local characteristics and allows more suitable control measures. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Is it atrophy of the gastric antropyloric mucosa or atrophy of the gastric body mucosa? a small doubt that may be beneficial to the patient

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

2011: International Year of Chemistry

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 14 Jan 2012 16:50:49 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Evaluation of hepatic glycogen correlated with serum glucose in castrated rats under tibolone treatment

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION:Tibolone administration in high doses and for a long period determines weight loss by dietary deficiency, which leads to liver function changes. Thus, it may affect glycogenolysis and gluconeogenesis with changes in liver glycogen and circulating glucose (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Nodular hyperplasia of Bartholin's gland: case reports and literature review

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

The Bartholin's gland (BG) may develop several tumoral lesions such as BG duct cysts, carcinomas, hyperplasias, and adenomas. BG nodular hyperplasias (NHs) are uncommon, and few cases have been reported in the literature. Specific criteria for the distinction between adenomas and hyperplasias have been recently established. Several questions on possible etiologies and appropriate medical approach still remain. Therefore, this work comprises three case reports of NH and an extensive literature reviewA glândula de Bartholin (GB) pode ser sede de várias lesões tumorais, como cistos do ducto da GB, carcinomas, hiperplasias e adenomas. A hiperplasia nodular (HN) da GB é uma lesão rara e poucos casos foram relatados na literatura. Recentemente, critérios específicos estabeleceram a difere...

Ghrelin- and preproghrelin-immunoreactive cells in atrophic body gastritis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: Ghrelin- and preproghrelin-immunoreactive cells are frequently present in endocrine hyperplasias associated with ABG. However, further studies are required to determine to what extent these hormones act as modulators of hyperplastic nodular growth and evolutionINTRODUÇÃO: Grelina é um peptídeo de 28 aminoácidos secretado principalmente pelas células endócrinas da mucosa gástrica, acreditando-se que apresente ação moduladora relacionada com o crescimento celular. OBJETIVO: Estudar a presença de grelina e da molécula precursora preprogrelina na hiperplasia endócrina associada à gastrite atrófica do corpo (GAC). MATERIAL E MÉTODOS: Biópsias endoscópicas de 54 pacientes com GAC foram processadas para imuno-histoquímica, e anticorpos específicos contra grelina, p...

Factors that affect the quality of DNA extracted from biological samples stored in paraffin blocks

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

Os avanços tecnológicos e metodológicos da biologia molecular criaram a possibilidade de obter DNA de amostras teciduais estocadas em blocos de parafina, alternativa esta que permite estudos retrospectivos de grandes bancos de tecidos contendo inúmeras doenças raras. O objetivo deste trabalho foi a realização de uma breve revisão de alguns fatores inerentes à qualidade do produto extraído proveniente de blocos de parafina armazenados. Os processos envolvidos nas fases de prefixação, fixação, pós-fixação apresentam aspectos que são causa de resultados de produtos finais insatisfatórios. Na fase de prefixação, alterações bioquímicas influenciam na preservação das macromoléculas. As mudanças moleculares causadas pelos fixadores para evitar autólise celular podem s...

Adenomyoepithelioma with malignant transformation diagnosed by immunohistochemical analysis: case report

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

Adenomyoepitheliomas (AMEs) are rare breast lesions, which are characterized by the proliferation of epithelial and myoepithelial cells. They are circumscribed lesions, which can be clinically and mammographically detected. However, when they are indistinctly limited, differential diagnosis with malignant lesions becomes difficult. In this paper, we report a case of adenomyoepithelioma with malignant transformation of the myoepithelial component, a rare histological type that arises from the malignant transformation of epithelial, myoepithelial, or both elements. Its course and prognosis are uncertain. Mastectomy with sentinel lymph node biopsy followed by radiotherapy are the most effective treatment approachesAdenomioepiteliomas (AMEs) são lesões raras na mama, caracterizadas pela prol...

Prevalence of non-fermenting Gram-negative bacilli among inpatients from Porto Alegre-RS

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: Three hundred twenty-six samples of non-fermenting Gram-negative bacilli were isolated. The most prevalent species were Pseudomonas aeruginosa (65.03%), Acinetobacter baumannii (16.56%), and Stenotrophomonas maltophilia (9.5%). Other species showed rates lower than 5%. The microorganisms were isolated from several infectious sites and the biological materials that showed higher positivity were the following: tracheal aspirate (38.34%), spittle (18.71%) and urine (15.95%). Bacterial resistance was higher with tetracyclines (89.57%) and sulfamethoxazole/trimethoprim (79.75%). The most active antimicrobials were polymyxin B and piperacillin/tazobactam with 100% and 75.2% sensibility, respectively (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Viral etiology of acute respiratory infections among children at Instituto Fernandes Figueira/FIOCRUZ/RJ

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: The viral prevalence among outpatients was 42.8% and among inpatients it was 30%; 83.3% of the children were carriers of one or more UD associated with ARI, resulting in long-term admission to hospital. Some children had multiple admissions and multiple clinical diagnoses of ARI during the studied period (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Stenotrophomonas maltophilia: emerging resistance to TMP-SMX in Brazilian isolates. a reality?

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: Although TMP-SMX is the standard treatment for S. maltophilia infections, there may be resistance to this antibiotic, which hinders the therapeutic approach, hence the significance of susceptibility tests. The disk diffusion technique showed a good correlation with microdilution. Among the new therapeutic options, both tigecycline and moxifloxacin presented significant activity in vitro (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Development of serum dexamethasone radioimmunoassay to corroborate Cushing's syndrome diagnosis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: The current RIA for serum Dx is accurate and reliable, which permits to establish a reference value range to substantiate DxST interpretation (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Rheumatoid arthritis: a current view

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

CONCLUSION: Despite the fact that there has been substantial progress in the investigation, diagnosis and treatment of RA, there are still several challenges to be overcome (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

A current view of JBPML publications in the field of Pathology

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 04 Nov 2011 02:45:57 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: The effect of Kint3-4 recombinant protein on tumor angiogenesis and control of malignant cell proliferation enhances the prospects of its use in clinical and antiangiogenic treatment.INTRODUÇÃO E OBJETIVO: A proteína Kint3-4 originou-se a partir de uma recombinação genética dos segmentos K1-3 e K1--4 do plasminogênio humano e é reconhecida por seu potencial anti-inflamatório e antiangiogênico. Este estudo teve como objetivo avaliar o efeito da proteína Kint3-4 no desenvolvimento de tumores em camundongos inoculados com células do tumor de Ehrlich. MÉTODOS: O fragmento de proteína foi obtido por uma técnica de clonagem e transformação de Pichia pastoris. Três diferentes protocolos foram avaliados após a inoculação das células tumorais: no início (0-6 dias)...

Fibrous pseudotumor of the spermatic cord: report of an unusual lesion

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

We report the case of 25 year-old patient, who presented painless scrotal mass. A total orchidectomy was performed. The anatomopathological diagnosis was fibrous pseudotumor of the spermatic cord. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

HPV oral infection and proliferative epithelial associated lesions

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

Os papilomavírus humanos (HPVs) pertencem à família Papillomaviridae e seu ciclo de vida é diretamente ligado à diferenciação das células epiteliais do hospedeiro. Possuem seis genes que se expressam precocemente e dois genes que se expressam tardiamente, sendo denominados respectivamente E (early) e L (late). O ácido desoxirribonucleico (DNA) viral dentro da célula do hospedeiro pode assumir duas formas: epissomal e integrada. O HPV tem como alvo as células basais de epitélios escamosos, em particular da área genital, onde está associado ao carcinoma da cérvice uterina. Na boca, o HPV está associado a papiloma escamoso oral, condiloma acuminado, verruga vulgar e hiperplasia epitelial focal. Entretanto, seu papel na carcinogênese oral é ainda controverso, sendo também id...

The dramatic decline of the autopsies at a Brazilian university hospital in the last 20 years

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: We believe that the main cause of this reduction is the lack of information among laypeople and medical professionals, who have neglected the fact that knowledge is ultimately acquired through study, investigation and practice. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Gallbladder carcinoma: clinicopathological study of 24 cases

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: There was a prevalence of advanced neoplasias diagnosed through anatomopathological examinations with poor prognosis. Furthermore, earlier detection contributed to higher survival rate. Investigation of anatomopathologic features, specially the macroscopic patterns of gallbladder carcinoma, is essential to improve diagnostic accuracy and to provide a definite treatment.INTRODUÇÃO: Não obstante estudos pré-operatórios, o câncer da vesícula biliar é frequentemente detectado durante a cirurgia ou no exame anatomopatológico após colecistectomias. OBJETIVO: Avaliar as principais apresentações clinicomorfológicas e a evolução de pacientes com carcinoma da vesícula biliar. MÉTODOS: Foram revisados prontuários médicos e material anatomopatológico de todos os pacient...

Phenol concentration evaluation in clinical analysis laboratory waste

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: It was possible to qualify and quantify phenol concentrations in both semi-automated and automated laboratory samples and the results revealed a decrease and subsequent stability in phenol concentrations. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Comparative study of human metapneumovirus detection by PCR and direct immunofluorescence methods

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: Not only does the study show that RT-PCR is the best method for HMPV detection in clinical respiratory samples but it also substantiates the importance of test standardization in laboratory routine. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Alternative methods for the detection of extended-spectrum-beta-lactamase in Escherichia coli and Klebsiella pneumoniae

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

CONCLUSION: Both methods yielded satisfactory results with higher sensitivity (90%) and lower costs in comparison with the reference one (CLSI disk diffusion method), which corroborates its use in ESBL investigation. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Multidrug-resistant Pseudomonas aeruginosa: an endemic problem in Brazil

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

Relatos mundiais têm documentado a problemática da endemicidade de isolados clínicos de Pseudomonas aeruginosa multirresistente (MDR) aliada a elevados índices de morbidade/mortalidade. No Brasil, surtos de infecção ocasionados por P. aeruginosa têm sido relacionados com uma disseminação clonal da espécie. Atualmente, as opções terapêuticas para o tratamento das infecções causadas por esse microrganismo são limitadas, muitas vezes restringindo-se ao uso de carbapenêmicos (p. ex., imipenem [IPM]). Assim, a resistência ao IPM é uma questão de saúde pública, uma vez que esse antibiótico é empregado como último recurso no tratamento de infecções de origem hospitalar, causadas por bactérias Gram-negativas multirresistentes. No Brasil, os principais mecanismos relacio...

Trends in laboratory medicine

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

A patologia clínica/medicina laboratorial é uma especialidade direcionada à realização de exames complementares no auxílio ao diagnóstico, com impacto nos diferentes estágios da cadeia de saúde: prevenção, diagnóstico, prognóstico e acompanhamento terapêutico. Diversos elementos apontam para maior utilização da medicina diagnóstica no futuro. Para discutirmos as principais tendências na medicina laboratorial, descrevemos os fatores que colaboram e são fundamentais para o crescimento desse mercado denominados, neste estudo, drivers de crescimento. As principais tendências que terão forte impacto na medicina laboratorial, e que serão descritas neste artigo, são: ferramentas de gestão, inserção de novos testes no mercado e rol de procedimentos, qualidade dos serviço...

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Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

2011: ano internacional de química

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 29 Sep 2011 18:16:28 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

The clinical laboratory in the investigation of hemoglobin disorders

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

This article aims to describe the laboratory diagnostic approach in four cases of hemoglobin disorders in order to illustrate the role of laboratories and discuss the role of clinical pathologists as a link between physicians and laboratories in diagnostic clarification. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Critical analysis of processes redesign experience in a clinical laboratory

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

CONCLUSION: Clinical laboratories may attain significant results with process redesign initiatives as long as these are result-oriented, aligned with the organization strategies and implemented with a planned approach. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Deployment model plan for managing waste in the clinical laboratory

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

CONCLUSION: The HSWMP facilitated communication, information dissemination, awareness, competence and training of all parties involved. Several activities were conducted simultaneously, which resulted in behavioral changes. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Hazards and risks in laboratory medicine: identification and evaluation

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

Os perigos no ambiente de trabalho estão relacionados com qualquer tipo de fonte potencialmente danosa, em termos de lesões, ferimentos ou danos para a saúde ou uma combinação desses fatores. Os riscos são consequências dos perigos existentes no laboratório. Os laboratórios clínicos apresentam múltiplos riscos ocupacionais aos trabalhadores, categorizados como riscos biológicos, físicos, químicos, ergonômicos e para ocorrência de acidentes. É importante o laboratório identificar os riscos, avaliar os impactos que podem afetar o negócio e estabelecer critérios de priorização para a tomada de decisões, implementando estratégias e ações preventivas, a fim de evitar a instalação de falhas ou danos potenciais. Este artigo propõe uma sistemática de identificação e...

Sustainability indicators in laboratory medicine

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

O artigo apresenta os princípios conceituais sobre desenvolvimento sustentável, sustentabilidade e avalia a evolução e os impactos na economia, no meio ambiente e na sociedade. Discutem-se a aplicabilidade dos conceitos de sustentabilidade empresarial na medicina laboratorial e os desafios inerentes à implantação no laboratório clínico. O impacto dos indicadores de sustentabilidade e seu papel no processo de gestão também são analisados criticamente sob a ótica do balanço socioambiental. O texto apresenta ainda algumas ferramentas para avaliação e interpretação dos indicadores e sua aplicação no processo de análise crítica. Finalmente, o artigo descreve a importância dos indicadores de sustentabilidade na prática do benchmarking e sua aplicabilidade no laboratório ...

How to implement the Plan of Care for Emergencies (LAP) in the clinical laboratory

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

CONCLUSION: It aims at preventing or mitigating occasional adverse consequences regarding safety, health and environment in clinical laboratories. RESULTS: This article discusses the applicability, responsibilities, development and maintenance as well as their corresponding implications in laboratory procedures (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

The importance of water quality in clinical laboratory reagent

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

A água é um reagente utilizado na maioria dos testes laboratoriais e por isso deve seguir um padrão de controle de qualidade rigoroso. O fornecimento urbano de água apresenta moléculas orgânicas, íons inorgânicos, partículas, coloides, gases, bactérias e seus produtos, que podem alterar os resultados dos exames laboratoriais e causar eventuais erros e falhas mecânicas em equipamentos analíticos. Para remover essas impurezas, é necessário recorrer a uma combinação de tecnologias de purificação. Há várias organizações que especificam normas sobre a água reagente, a fim de minimizar sua interferência nos ensaios laboratoriais. A maioria dos laboratórios utiliza as normas estabelecidas pelo Clinical and Laboratory Standards Institute (CLSI) que classifica a água em: c...

The clinical laboratory free of mercury

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

CONCLUSION: A mercury-free laboratory environment requires managerial commitment, a consistent action plan and staff awareness of health and environmental risks. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Usefulness of quality indicators in the management of clinical laboratories

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

The objective of the present study is to discuss the evolution of quality, mainly in the laboratory area, focusing on the importance of quality indicators in laboratory management. Some pre-analytical, analytical and post-analytical laboratory indicators are also presented and discussed in this work. Finally, we highlight the Brazilian initiative in the Laboratory Indicator Program developed by the Brazilian Society of Clinical Pathology and Laboratory Medicine (SBPC/ML) in partnership with Control-Lab and the Model of Quality Indicator project, which has been developed by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Quality, indicators, and sustainability

Jornal Brasileiro de Patologia e Medicina Laboratorial — Wed, 17 Aug 2011 06:06:03 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Epstein-Barr virus (EBV) detection in gastric carcinomas from Ceará and São Paulo states, in Brazil

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: The frequency of EBV(+) tumors is similarly described in the literature. Among our findings, the elevated percentage of EBV(+) tumors in the gastric corpus, which was observed in São Paulo cases, is unprecedented in the literature (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Twin pregnancy with complete hydatidiform mole and living fetus

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

This study reports the case of a 20 years old primiparous patient with complete hydatidiform mole and living fetus, whose pregnancy was successfully carried to term. The differential diagnosis with placental degeneration was reached through anatomopathological investigation (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Evaluation of M.I.C.E.TM, Etest® and CLSI broth microdilution methods for antimicrobial susceptibility testing of nosocomial bacterial isolates

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: AST results obtained from M.I.C.E. methodology showed a good correlation with those from broth microdilution and Etest, which corroborates its time effectiveness in the determination of MIC. However, the combination of amoxicillin/clavulanic acid requires further attention (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Staphylococcus lugdunensis: a different view in the clinical laboratory

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

Os estafilococos coagulase negativos (ECNs) são cocos Gram-positivos usualmente considerados contaminantes em laboratórios de microbiologia clínica. Apesar de pertencer a este grupo, Staphylococcus lugdunensis pode causar infecções complicadas, como endocardites, infecções de pele e tecidos moles, osteomielites, entre outras. Além da formação de biofilmes, apresenta patogenicidade similar ao Staphylococcus aureus. É um dos principais agentes causadores de endocardites, com taxa de mortalidade de até 70%. Pode ser confundido com S. aureus quando se utilizam testes rápidos para sua identificação, como a pesquisa de clumping factor, no caso de teste de coagulase em lâmina, ou em testes de aglutinação direta em látex. Pode ser facilmente identificado por meio de provas bioqu...

Effects of anticoagulants and storage temperature on blood nitrite levels

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

A mensuração dos metabólitos do óxido nítrico pode ser útil para a melhor compreensão de diferentes processos fisiopatológicos. Este estudo avaliou a influência de diferentes anticoagulantes (ácido etilenodiaminotetracético [EDTA], citrato e heparina) e do armazenamento a -20ºC por quatro meses sobre os níveis de nitrito, sendo estes mensurados em amostras de soro e plasma pelo método de Griess. Os tipos de amostra utilizados (soro ou plasma) e de anticoagulante usado na coleta não influenciaram significativamente os níveis de nitrito, independentemente de as dosagens serem realizadas em amostras frescas ou naquelas mantidas a -20ºC por quatro meses.The measurement of nitric oxide metabolites may be useful for better understanding of different physiopathological processes....

Comparison of methods to determination of vancomycin sensivity in methicillin-resistant Staphylococcus aureus

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: The Etest® method overestimates the MIC values, however it is easily and quickly accomplished. In the other hand, broth macrodilution is very laborious and time consuming. MicroScan Walk Away 96 (Siemens®) results are accurate, but does not provide the exact MIC value requiring an additional test to estimate the effectiveness of treatment. In conclusion, the Etest® can be used as an alternative for routine monitoring. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Comparative study between methods of screening for detection of hemoglobin S in the blood banks

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: Hemoglobin electrophoresis proved the best method in the identification of hemoglobin variants and, therefore, worthwhile when it comes to diagnostic screening in blood banks due to its high sensitivity, which keeps false-negative results to a minimum and ensures blood quality. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Serological screening and the influence of dengue awareness on patients from SUS specialty laboratory

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: All patients showed disease and prevention awareness regardless of their socio-economic background. The asymptomatic infection must be evaluated mainly in cases of disease with fever. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Concepts in laboratory medicine automation: literature review

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

This article aims at briefly reviewing the automation processes available in laboratory medicine. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Triglycerides/HDL-C ratio and high sensible C-reactive protein to the evaluation of cardiovascular risk

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

CONCLUSION: The triglycerides/HDL-C ratio and hs-CRP may be used as indicators of increase in cardiovascular risk among women with coronary artery disease (CAD). (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Critical reflections on the three R's or the brazilian journals deleted

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

World Kidney Day 2011 Proteinuria and serum creatinine: critical tests for diagnosis chronic kidney disease

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Our journal cover -2011: International Year of Chemistry

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 27 May 2011 16:30:11 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Epidermoid cyst of the posterior fossa: a case report

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:45 +0100

Epidermoid cysts of the central nervous system are uncommon conditions, which are frequently located in the cerebellopontine angle and around the pons. They are covered with keratinized squamous epithelium and keratin lamella, which give its contents a soft, white-pearly appearance. Epidermoid cysts are mostly originated from malformations, presumably associated with surface elements of the nervous system ectoderm during the closure of the neural groove or formation of secondary cerebral vesicles. The authors describe a case of epidermoid cyst in the posterior fossa causing hydrocephalus and review morphologic and diagnostic criteria of this lesion.Os cistos epidermoides do sistema nervoso central (SNC) são condições incomuns, estando localizados mais frequentemente no ângulo pontocere...

Increase in perivillous fibrinoid material in placentas from pregnancies with preeclampsia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:45 +0100

CONCLUSION: In placentas from preeclampsia pregnancies the fibrinoid material area is larger (1.8x) compared to normal pregnancies. Nuclei and cytoplasm areas were larger in the control group. There was no statistical difference regarding the stromal area. There was also a reduction in villous space in preeclampsia, which is consistent with villous hypotrophy. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Cutaneous Rosai-Dorfman disease: case report and review of literature

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

We report a patient with cutaneous Rosai-Dorfman disease that presented on the right buttock, a red brown plaque with yellowish areas and red-brown satellite papulas. The histopathologic exam revealed inflammatory infiltrate predominantly of lymphocytes and histiocytes with wide and clear cytoplasm containing intact inflammatory cells (emperipolesis). Any other alteration in the physical exam and laboratorial was not found. The Rosai-Dorfman disease is a rare form of histiocytic proliferation and the strictly cutaneous form is still rarer. The aetiology is unknown and the clinical and histologic correlation enables the correct diagnosis. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Immunohistochemical evaluation of 100 cases of encephalic metastases and correlation with primary site of the tumor

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: The set of morphological findings associated with immunohistochemical study of an encephalic metastatic lesion may suggest the primary site in several cases, which, therefore, requires the appropriate choice of antibody panel. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Galectin-3 and beta-catenin expression in premalignant and carcinomatous lesions in tongue of mice

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: Wnt signaling pathway is active in both dysplasias and carcinomas and the increase of nuclear galectin-3-positive cells in carcinomas suggests its influence on malignant transformation in the tongue epithelium. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Immunomodulatory effects of oral antidiabetic drugs in lymphocyte cultures from patients with type 2 diabetes

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: We demonstrated that diabetes itself may reduce cell proliferation significantly when stimulated by PHA, which may indicate that diabetic patients have difficulties in promoting an efficient inflammatory response. Moreover, the use of oral hypoglycemic drugs may aggravate this situation.INTRODUÇÃO E OBJETIVOS: Tem sido sugerido que o diabetes mellitus tipo 2 (DM2) é uma manifestação da resposta inflamatória. As principais drogas utilizadas no tratamento do DM2 são as sulfonilureias e as biguanidas. O objetivo deste trabalho é demonstrar os efeitos moduladores na proliferação de linfócitos causada pelos hipoglicemiantes orais (clorpropamida e metformina), in vitro e ex vivo. MÉTODOS: Células mononucleares de sangue periférico foram isoladas de seres humanos por gra...

Evaluation if in-house preparations of polled plasma can be used as a normal control of partial thromboplastin time (PTT)

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: We found that in-house preparations of polled plasma (P5 and P20) can be used as a normal control of PTT. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Comparison of susceptibility profile among clinical isolates of oral and vulvovaginal Candida spp. in southern Brazil

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: The preliminary assessment by in vitro susceptibility testing should guide the conduct of effective therapy for cases of recurrent mainly in areas of high prevalence of opportunistic infection, as in western of Santa Catarina. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Phenotypes of beta-lactamases in Klebsiella pneumoniae from emergency hospital of Porto Alegre

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: Research of resistance phenotypes in Klebsiella is easy and feasible tool in microbiology laboratories, and is fundamental to understanding the local epidemiology of beta-lactamases, allowing the appropriateness of antimicrobial therapy more convenient. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Serum creatinine, cystatin C, and β-trace protein in diagnostic staging and predicting progression of primary nondiabetic chronic kidney disease

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

CONCLUSION: The diagnostic performance of serum creatinine, cystatin C, or BTP for detecting even minor degrees of deterioration of renal function is good, and these markers provide reliable risk prediction for progression of kidney disease in patients with CKD. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Serum creatinine and glomerular filtration rate: perception and reality

Jornal Brasileiro de Patologia e Medicina Laboratorial — Tue, 22 Mar 2011 20:59:44 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Correlation of histopathological patterns in cutaneous melanoma with BRAF mutations

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: As far as mutational status is concerned, it was not possible to confirm any predictive value for histopathological patterns such as circumscription, presence of isolated melanocytes in the lesion and cytological features. Difficulties in the interpretation of some histological criteria were demonstrated by the variation in the observers' conclusions. It is difficult to state if genetic alterations such as BRAF mutations may serve as biomarkers for melanoma classification.INTRODUÇÃO: Mutações do gene BRAF localizado no cromossomo 7q são as mais frequentemente encontradas em melanomas cutâneos (60%-80%). O único estudo que correlacionou padrões histopatológicos de melanomas cutâneos com a presença de mutações BRAF foi realizado por Viros et al., em 2008, que observa...

Colloid cyst in the third ventricle and sudden death in young man

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

Morte súbita de jovem, sexo masculino, 23 anos, assintomático, suscitou verificação de óbito. Antes queixou-se de cefaleia excruciante, em sala de aula, caindo sobre o computador. Encéfalo apresentou edema e congestão vascular. Sem herniações. Cortes coronais evidenciaram dilatação dos ventrículos laterais e nódulo aderido ao teto do terceiro ventrículo. À microscopia o diagnóstico foi cisto coloide do terceiro ventrículo. A ameaça de morte súbita em portadores de cisto coloide é sério problema diagnóstico na emergência médica. A relevância deste caso está em lembrar aos médicos que esta entidade deve permanecer como diagnóstico diferencial nas cefaleias em crianças, adolescentes e adultos jovens.Sudden death of 23 year-old asymptomatic male patient led to furt...

BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: The identification of both BRAF and c-Kit mutation is not a suitable prognostic indicator in the Brazilian population. Moreover, the relatively low frequency of BRAF mutations brings into question if it actually plays a key role in melanoma pathogenesis.INTRODUÇÃO: Técnicas de biologia molecular permitem a identificação de marcadores moleculares como mutações dos genes BRAF e c-Kit em melanomas. Estudos de alterações genéticas em pacientes sul-americanos são escassos. OBJETIVOS: Identificar a incidência de mutações dos genes BRAF e c-Kit em melanomas cutâneos primários em uma série de pacientes brasileiros e avaliar as implicações patogenéticas e prognósticas dessas mutações, correlacionando-as com dados clínicos e histopatológicos. MATERIAL E MÉTODOS:...

Oral and laboratorial findings in patients with systemic lupus erythematosus

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: These data suggest that patients with SLE present low prevalence of oral lesions and poor periodontal conditions. Moreover, the cytopathology showed infections associated with immunosuppression such as candidiasis and oral hairy leucoplakia. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Validation of tissue microarray technique for intratubular epithelial dysplasia study

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: TMA validation assay demonstrated that the use of two tissue cores with 1 mm² each was suitable to evaluate RCC and IED. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Possible effects of blood copper on hematological parameters in elderly

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: The results showed that copper, although it is within accepted reference values, may be involved in ALA-D inhibition, which may affect hematological parameters such as hemoglobin synthesis. Thus, the reference levels for copper in the elderly should be reviewed. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Rat brain polyamines: an analytical method validation

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

The validation of the analytical technique for the determination of polyamines in cerebral tissue using HPLC based on o-phthalaldehyde post-column derivatization is described. The polyamines were separated in a LiChrospher100 RP18 column. Elution gradient was formed with two mobile phases: A (sodium acetate 0.1 M + sodium octanesulphonate 0.01 M, pH = 4.5) and B (sodium acetate 0.2 M + sodium octanesulphonate 0.01 M)/acetonitrile (10:3), pH = 4.5) in a 1.2 ml/min flow rate. The derivative eluent was monitored by fluorescence (excitation, 345 nm; emission, 455 nm). Besides excellent linearity (putrescine, r = 0.9816; spermidine, r = 0.9920; spermine, r = 0.9901), the technique demonstrated intra and inter-day precision (< 20%) as well as recovery (spermidine = 92.56%; spermine = 84.47%). Qu...

Cystatin C measurement as renal function marker

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: The nephelometric cystatin C assay may be perfectly suitable for our routine laboratory. The correlations between serum creatinine and the various equations for estimating glomerular filtration are better than those between cystatin C and equations for estimating glomerular filtration in the three groups irrespective of the immunosuppressive therapy used. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Distribution of β-thalassemia mutations in Fortaleza, Ceará

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

CONCLUSION: These results demonstrate the profile of β-thalassemia mutations in the Northeast region, thus contributing to the study of their distribution in Brazil. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

O laboratório clínico e os intervalos de referência

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Our Journal Cover - Pissarro: painter of the land

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 14 Jan 2011 19:37:49 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Investigation of the LMP1 EBV and co-infection by HPV in genital lesions of patients infected or not by HIV

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

CONCLUSION: The results demonstrated the possibility of synergism between EBV infection and EBV-HPV co-infection in genital epithelial lesions, mainly among HIV-infected patients. However, further investigations with a more specific and sensitive methodology are required in order to assess the real influence of EBV on the pathogenesis of genital epithelial lesions. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Antiobesity effects of green tea (Camelia sinensis) in high caloric diet-induced obese rats

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

CONCLUSION: It was observed a reduction in body weight and in liver steatosis as well as an improvement in GTT. There was also an increase in IR phosphorylation in treated animals in comparison with non-treated ones. Lesions such as focal necrosis were observed and may be associated with the treatment. The immunohistochemical analysis demonstrated the presence of IR and Shc in all groups and suggested changes in IR and Shc expression in both treated and non-treated groups. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Primary sclerosing cholangitis in children and adolescents: a clinicopathologic study with a proposal of criteria for early diagnosis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

CONCLUSION: Our study emphasizes that even when CG is normal, PSC should be exclusively diagnosed by liver biopsy, hence cholangiography being unnecessary. Chronic portal inflammation, neutrophilic pericholangitis, periductal sclerosis and "onion skinning" are characteristic histopathological findings. Neutrophilic pericholangitis may be subtle and easily overlooked in early disease, leading to strong suspicion of PSC.INTRODUÇÃO: Colangite esclerosante primária (CEP) é crescentemente diagnosticada em crianças e adolescentes devido ao melhor reconhecimento das apresentações clínicas por imagem e manifestações patológicas. Devido a isso, aumentaram a sensibilização e a melhora das imagens e, cada vez mais, os pacientes são diagnosticados em idade mais jovem. OBJETIVO: As primei...

Evaluation of external quality assessment in clinical laboratories from Rio de Janeiro in the years 2006-2008

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

In this study, we evaluated the percentage distribution of the results of health inspections conducted in CL in the State of Rio de Janeiro from 2006 to 2008, registered at SESDEC, stating the number of CL that did not meet health requirements (item: "External Quality Control -EQC-Directive 743/2006") and the nature and the sector of these laboratories: intra and extra hospital clinical laboratories (publicly or privately owned hospitals and outsourced hospitals). OBJECTIVE: Statistic evaluation of EQC in CL in the State of Rio de Janeiro during the period under review as to the nature of these laboratories. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Does soy increase blood counts in myelodysplastic syndromes?

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

Myelodysplastic syndromes (MDS) are a group of clonal stem cell diseases characterized by ineffective hematopoiesis, bone marrow hyperproliferation, cytopenias in peripheral blood and risk of transformation into acute leukemia. We decided to investigate the effects of a soy concentrate on MDS patients based on the follow-up results of a 61 year-old Japanese female patient who was diagnosed with MDS and refractory cytopenia with multilineage dysplasia in 2003 (hemoglobin = 11g/dL; white blood cells count = 2,500/uL and platelets = 25,000/uL; marrow with mild dysplasia and normal karyotype; paroxysmal nocturnal hemoglobinuria was excluded). She started using soy as a dietary supplementation in May 2004 and presented a gradual increment in blood counts, achieving normalization approximately e...

Collection, transport and storage of samples for molecular diagnosis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

In this study we highlight that proper care during the pre-analytical phase is extremely important in order to ensure the reliability of molecular diagnostic tests. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Seroepidemiology of HTLV-I/II infection among population assisted by Family Health Program in Salvador-Bahia, Brazil

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

CONCLUSION: The seroprevalence found is considered high compared to other Brazilian municipalities. The analyzed risk factors were not statistically different between HTLV-I/II seronegative and seropositive groups. The vertical transmission was not an important factor for virus maintenance. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Serratia marcescens KPC sepsis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

In this study we describe a case of sepsis caused by Serratia marcescens carrying blaKPC gene. In Brazil, cases of KPC have been reported in Klebsiella pneumoniae and Escherichia coli, which shows the emergence of this kind of carbapenemase and its dissemination among different species of Enterobacteriaceae in our country. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Quality control in clinical laboratory: aligning process improvement, reliability and patient safety

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

CONCLUSION: The new proposed approach, which is a complement to the traditional quality control system and involves the sequential and associated use of different statistical tools, proved to be a valid and useful model for effective performance evaluation and its ongoing performance stability. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Controle de qualidade no laboratório de análises clínicas

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Our journal cover - Edgar Degas: fascination with movement

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 31 Oct 2010 14:50:54 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Our journal cover - Cézanne: the founder of modern art

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 18 Sep 2010 07:34:51 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Ghrelin and pre-proghrelin immunoreactive cells in gastric neuroendocrine tumors associated with atrophic body gastritis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: Ghrelin and preproghrelin immunoreactive cells may be found in variable number in Type I neuroendocrine gastric tumors and in hyperplastic nodules associated with these tumors. However, it remains unclear what role these peptides play on the development of these tumors.INTRODUÇÃO: Grelina é um peptídeo de 28 aminoácidos, reconhecido como liberador endógeno do hormônio do crescimento, sendo secretado principalmente por células endócrinas da mucosa gástrica. A expressão imuno-histoquímica da grelina tem sido descrita em tumores neuroendócrinos, acreditando-se que possa ter ação moduladora relacionada com o crescimento desses tumores. OBJETIVO: Estudar a presença de células imunorreativas a grelina e pré-progrelina em tumores neuroendócrinos gástricos associado...

Animal type melanoma: a report of two cases

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

Dificuldade potencial no diagnóstico histológico de melanomas é a dificuldade em reconhecer variantes pouco frequentes de melanoma. Entre elas, as mais desafiantes incluem exemplos de melanoma desmoplásico, melanoma nevoide, o chamado "melanoma de desvio mínimo", melanomas com proeminente síntese de pigmento ou "melanoma tipo animal" e o nevo azul maligno. Os autores descrevem dois casos de melanoma tipo animal e discute-se a importância do diagnóstico diferencial clinico-histopatológico nesses casos.A potential diagnostic pitfall in the histological assessment of melanomas is the difficulty in recognizing unusual melanoma variants. Among them, the most challenging examples comprise desmoplastic melanomas, nevoid melanomas, the so-called minimal-deviation melanoma, melanomas with ...

Preliminary study of using otoliths of Cynoscion acoupa upon the process of bone regeneration in rats

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: These data suggest that otoliths may be an adjuvant to the dynamics of bone regeneration. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Detection of phenotypic metallo-beta-lactamase in clinical isolates of Pseudomonas aeruginosa from hospitals in Caxias do Sul

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: The presence of MBL is an important resistance mechanism found in the studied hospitals, which substantiate the need for the detection of these enzymes in order to control the spread of resistance genes and provide appropriate therapy to the patient. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Immunophenotypic characterization of plasma cells in patients with multiple myeloma

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: From this study it was concluded that the aberrant phenotypes are present in most cases of MM, and immunophenotyping by multiparametric flow cytometry is a useful tool to distinguish normal plasma cells from myeloma plasma cells. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

The Gardnerella vaginalis and the urinary tract infections

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

As infecções do trato urinário (ITUs) estão entre as mais frequentes nos seres humanos. São causadas por grande variedade de uropatógenos habituais, porém podem ser provocadas por alguns micro-organismos fastidiosos, como a Gardnerella vaginalis, que é uma bactéria anaeróbia facultativa, observada sob a forma de cocobacilos Gram-variáveis. Ela habita a mucosa vaginal e eventualmente pode ocasionar ITUs. O isolamento pode ser realizado em amostras de urina utilizando o ágar CNA (colistina e ácido nalidíxico), com incubação de 48 a 72 horas em atmosfera rica em CO2. O exame de Gram de urina não centrifugada pode auxiliar o microbiologista na identificação das amostras nas quais uma bactéria fastidiosa seja o agente causal, já que a visualização de inúmeras células ep...

When and how to enhance polymicrobial urine cultures at the laboratory of clinical microbiology

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: It is important to emphasize the correlation between laboratory findings and patients' clinical indications. It is recommended that polymicrobial isolates in urine samples from both out-patients and inpatients should be thoroughly investigated. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Prevalence of high serum uric acid is increased in ambulatory subjects with hyperglycemia and dyslipidemia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

CONCLUSION: These results show the association between serum levels of uric acid and metabolic syndrome profile, which corroborates to similar results found in other populations worldwide.INTRODUÇÃO: O ácido úrico sérico tem sido considerado um marcador ou componente das alterações clínicas e laboratoriais da síndrome metabólica. OBJETIVO: Avaliar a associação entre o ácido úrico sérico (AU) e o perfil laboratorial composto de glicemia de jejum > 100 mg/dl, triglicerídeos > 150 mg/dl e colesterol da lipoproteína de alta densidade (HDL-C) < 50 mg/dl nas mulheres e < 40 mg/dl nos homens. MÉTODO: Em estudo de corte transversal, amostras de sangue de 4.328 pacientes ambulatoriais não selecionados com idade variando de 20 a 102 anos foram examinadas. RESULTADOS: A media...

The clinical and laboratory importance of mean platelet volume

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

Os índices plaquetários fornecidos pelos analisadores hematológicos são provavelmente os parâmetros mais ignorados pela maioria dos laboratórios clínicos, em virtude da dificuldade de sua padronização. Desses índices, o volume plaquetário médio (VPM) vem merecendo destaque por sua grande utilidade, não só em casos de trombose e hemostasia, mas também em uma série de patologias, como diabetes, doenças da tireoide, doenças vasculares, entre outras. O VPM é um parâmetro plaquetário fornecido no hemograma que não gera custos adicionais para o laboratório. Junto com a contagem de plaquetas, ele é um sensível indicador de desordens plaquetárias in vivo, mas pode ser tecnicamente difícil de analisá-lo in vitro por causa dos interferentes pré-analíticos, como tempo de...

Change the QUALIS criteria

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Cézanne: the founder of modern art

Jornal Brasileiro de Patologia e Medicina Laboratorial — Thu, 09 Sep 2010 07:06:03 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Evaluation of the predictive factors involved in the neoplastic invasion of the nipple-areolar complex in patients with breast cancer

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: The results of this study enabled us to identify factors associated with an increased risk of neoplastic NAC invasion. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Bone tissue histomorphometry in castrated rats treated with tibolone

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: Oophorectomy caused bone loss in the analyzed regions. Despite the fact tibolone did not augment bone mass, this was kept at satisfactory levels. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Phisiopathogenesis, epidemiology and laboratory diagnosis of candidemia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

This study aimed at providing a review of candidemia, showing some important aspects of etio-epidemiology, fungus/host relation, and laboratorial diagnosis of this emerging systemic infection. During the onset of candidemia, yeasts play a major role on several virulence factors, which ultimately promote its pathogenesis. Among them, it may be highlighted the extracellular enzyme production, dimorphism, hemolysin synthesis, adhesion ability and biofilm development. In infected patients, medicines must be administered in the early stages to minimize the risks of complications and antimicrobial resistance. Therefore, an efficient laboratory diagnosis is required. The study of these factors associated with genotype analysis may contribute decisively to the discovery of new substances that may ...

Methodology optimization for KIR genotyping

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

The objective of this work was to optimize KIR genotyping method described by Martin (2004). It was used PCR-SSP (polymerase chain reaction-sequence-specific primers) with primers synthesized by Invitrogen® and visualization of the amplified products on 2% agarose gel electrophoresis, containing ethidium bromide. Some adaptations were made and the reagents had their concentrations increased: the internal control from 100 nM to 150 nM, forward and reverse specific primers KIR12.5/12.3, KIR13.5/13.3, KIR14.5/14.3, KIR22.5/22.3 and KIR36.5/36.3 from 500 nM to 750 nM, and MgCl2 solution from 1.5 mM to 2 mM. Other reagent concentrations and amplification temperatures were maintained. Satisfactory results were obtained with Taq DNA Polymerase Recombinant (Invitrogen®). The results of seventy s...

Endothelial lesion markers dosage in chronic renal disease patients undergoing hemodialysis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: Patients undergoing hemodialysis have high endothelial lesion, nevertheless, the presence of HCV chronic infection did not prove to be an aggravating factor. This result may be due to the small number of patients, hence further analyses with a larger sample are required for definitive conclusions. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Current issues in measurement and report of urinary albumin excretion

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

DISCUSSION: Clinical needs have been identified for standardization of (a) urine collection methods, (b) urine albumin and creatinine measurements based on a complete reference system, (c) reporting of test results, and (d) reference intervals for the ACR. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Proposal for the use of a pool of whole blood as internal quality control in hematology

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: We could verify that the pool of whole blood prepared in accordance with the methodology proposed does not present the required stability to be used as an alternative internal control in the Hematology division. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

In vitro antimicrobial activity of plant extracts against Enterococcus faecalis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: All the substances analyzed showed antimicrobial activity against E. faecalis. Therefore , they may represent alternative substances in endodontic treatment. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Improvement of an HPLC method to determine urinary δ-aminolevulinic acid

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

CONCLUSION: The method was considered suitable for the routine analysis of ALA-U.INTRODUÇÃO E OBJETIVO: A proposta deste estudo foi avaliar e aprimorar uma metodologia de cromatografia líquida de alta eficiência (CLAE)(11, 12), a fim de determinar o ácido δ-aminolevulínico urinário (ALA-U) utilizando volumes reduzidos de amostra. MÉTODO: O método baseia-se na formação de um composto fluorescente e posterior corrida cromatográfica de 15 minutos. RESULTADOS: O método apresentou linearidade, precisão e recuperação adequadas. Os resultados para as amostras de urina testadas foram 1,2 ± 0,9 mg/l (média ± desvio padrão) de ALA-U. CONCLUSÃO: O método foi considerado adequado para análises de rotina de ALA-U. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Proteinúria: muito mais que uma simples dosagem

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Monet: father of impressionism

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 21 Aug 2010 07:26:38 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Analytical methodology validation and stability study for serum quantification of acetaminophen

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

CONCLUSION: The method was precise, accurate and robust and showed good recovery. The control-samples were stable in all tested conditions. The method developed presented all the necessary parameters to be applied in acetaminophen quantification in plasma samples or human serum for emergency analyzes. Furthermore, it is a simple, time and cost-effective technique. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Density of Langerhans cells in the keratocystic odontogenic tumor

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

CONCLUSION: The lower number of LCs observed on atrophic cystic epithelium of KOTs may be due to decreased epithelial immunosurveillance and this may result in locally aggressive invasiveness.INTRODUÇÃO: Tumor odontogênico queratocístico (TOQ) é uma lesão odontogênica de caráter distinto que afeta frequentemente ossos maxilares. Células de Langerhans (CLs) são células dendríticas especializadas, responsáveis pela apresentação de antígenos aos linfócitos T nas superfícies cutânea e mucosa. OBJETIVO: Este estudo analisou a expressão imuno-histoquímica das CLs em lesões de TOQ. MATERIAIS E MÉTODOS: Quinze casos de TOQ foram estudados utilizando o marcador anti-CD1a. RESULTADOS: As CLs foram observadas em todos os 15 casos analisados. Essas células estavam concentradas ...

Oral lymphoepithelial cyst: report of 4 cases and review of 119 cases reported in the literature

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

CONCLUSION: The analysis of the cases herein and the other 119 cases reported in the literature reveals that oral lymphoepithelial cysts present a predilection for males, with peak of incidence in the third decade of life. Characteristically, these lesions consist of small asymptomatic nodules located in the buccal floor. Histologically, most cases show a parakeratinized epithelial lining and germinal centers in the fibrous capsule. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Small cell variant of squamous cell carcinoma vs. small cell carcinoma of the lung: differential diagnosis in biopsies

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

O diagnóstico diferencial entre a variante pequenas células do carcinoma epidermoide e do carcinoma de pequenas células nem sempre é fácil. Apesar de os descritores alertarem que o primeiro deva manter suas características morfológicas e, muitas vezes, diferenciação escamosa focal, a escassez de material aliada a artefatos de fixação frequentes nessas biópsias podem dificultar a vida do patologista. Entretanto, a definição entre um e outro pode alterar significativamente a escolha da modalidade terapêutica do paciente e, em alguns casos, influenciar seu prognóstico. Procuramos nesta publicação alertar para o problema e facilitar essa diferenciação, sugerindo um painel imuno-histoquímico.The differential diagnosis between small cell variant of squamous carcinoma and sma...

Epithelial trophoblastic tumor associated with in situ squamous cell carcinoma of the cervix

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

Epithelioid trophoblastic tumors are rare neoplasias that develop from the neoplastic transformation of chorionic-type intermediate trophoblasts. They show a wide spectrum of differential diagnoses. The authors describe a case of epithelioid trophoblastic tumor with atypical presentation in a 26-year-old patient that had recently undergone conization for in situ squamous cell carcinoma of the uterine cervix. The histopathology, immunohistochemistry and differential diagnoses of the lesion are also discussed. No similar case had been reported in the medical literature and it is an example of the hindrances to the diagnosis of epithelioid trophoblastic tumors.Os tumores trofoblásticos epitelioides são neoplasias raras que se desenvolvem a partir da transformação neoplásica de trofoblast...

Immunohistochemical assessment of E-cadherin, β-catenin, CEACAM-1 and PTEN: tumor progression markers in melanoma

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

CONCLUSION: There was a higher positivity for E-cadherin, b-catenin and PTEN in thin melanomas, whereas there was a higher positivity for CEACAM-1 in thick melanomas.INTRODUÇÃO E OBJETIVO: O objetivo do presente estudo está na avaliação imuno-histoquímica de marcadores de progressão tumoral (E-caderina, β-catenina, CEACAM-1 e PTEN) em melanomas cutâneos primários e sua correlação com fatores prognósticos. MÉTODO: Lesões primárias de pacientes portadores de melanoma cutâneo foram tabuladas quanto a dados clínicos (idade, sexo, localização e metástases) e anatomopatológicos (Breslow, nível de Clark, margens, tipo histológico, mitoses, ulceração, regressão, satelitose e tipo de TIL). Foi realizada comparação da expressão imuno-histoquímica dos marcadores em estu...

Borderline ovarian endometrioid tumor in adolescent: case report and review of the literature

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

Os tumores endometrioides malignos ovarianos são neoplasias epiteliais raras em pacientes jovens. Enquanto os carcinomas endometrioides ovarianos acometem mulheres na quinta e na sexta décadas de vida, os tumores borderline acometem mulheres em uma faixa etária mais ampla (de 22 a 77 anos). Descreveremos a seguir um caso raro de tumor endometrioide borderline ocorrendo em paciente de 16 anos. Desde que tumores do saco vitelino (TSV) e de Sertoli-Leydig (TCSL) podem mimetizar morfologicamente tumor endometrioide, o uso da imuno-histoquímica, além dos aspectos morfológicos, foi fundamental para estabelecer o diagnóstico diferencial entre essas condições.Malignant endometrioid ovarian tumors are rare epithelial neoplasias among young patients. Whereas endometrioid ovarian carcinomas ...

Mucinous invasive carcinoma of the breast and its differential diagnosis by core biopsy: review of the literature

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

In conclusion, core biopsy is a reliable technique for the diagnosis of mucinous carcinoma of the breast and its differential diagnosis, however, in doubtful cases or when the sample is scarce, it is advisable to perform an excisional biopsy to clarify the diagnosis. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Difficulty on laboratory identification of alpha thalassemia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

Discussion and conclusion: The results show that the presence of Hb H, which is indicative of alpha thalassemia, may not be confirmed in a subsequent analysis. Iron deficiency in Hb H carriers is among the factors that may influence on the absence of Hb H in alpha thalassemia proven patients. Alpha thalassemia is associated with genetic defects involving alpha chain encoding genes, but may be also associated with a temporary imbalance of globin gene expression, alpha chain reduction or beta increase, which could explain the presence of beta chain tetramer (Hb H) leading to the diagnosis of alpha thalassemia minor. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Comparison between two methods for human papilomavírus DNA detection in lip squamous cell carcinoma

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

Conclusion: HPV DNA detection of lip SCC increased sixfold in the studied cases with the employment of nPCR. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Carcinoma mucinoso invasor da mama e seus diagnósticos diferenciais na biópsia percutânea com agulha grossa

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Renoir: pain fades, beauty remains

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sun, 18 Jul 2010 06:24:02 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

The relation between Gleason score and prognostic factors in acinar adenocarcinoma of prostate

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

CONCLUSION: In prostate adenocarcinomas, the determination of Gleason score corresponds to one of the main histological factors to be described, showing significant relation with other anatomopathological criteria, such as extension, perineural and angiolymphatic invasion and staging. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Abdominal desmoplastic small round cell tumor of childhood: case report

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

We report a case of DSRCT affecting an 11-year-old male patient, with intra-abdominal involvement characterized by a large retroperitoneal mass in left hypochondrium. Histological examination of the mass showed the presence of clusters of small blue round tumor cells surrounded by a dense desmoplastic stroma. Immunohistochemical analysis disclosed a positive reaction to desmin, WT-1 and cytokeratins. After diagnosis, the patient underwent chemo radiotherapy treatment, but died at the 24th month of follow-up. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Evaluation of alternative technique combinations of building and preparation of tissue microarrays blocks

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

CONCLUSION: It was concluded that the combination choice depends on the number of samples to be put in TMA blocks. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Intravascular large B cell lymphoma: impairment of bilateral adrenal in young woman

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

We report a case of IVLBCL in a young female patient with bilateral involvement of the adrenal gland. Immunohistochemical examination confirmed IVLBCL. The relationship between endocrine organs and lymphoid cells is a hypothesis to explain the correlation observed between IVLBCL and the involvement of the adrenal gland. Moreover, the association between IVLBCL and the described symptomatology seems to reflect a pattern that may assist in a more effective diagnosis. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Primitive neuroectodermal tumor of the urinary bladder: a rare neoplasm

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

Os autores relatam o caso de paciente do sexo feminino, 52 anos, com queixas de hematúria macroscópica e dor pélvica. Foi realizado exame ultrassonográfico, que mostrou massa expansiva em assoalho vesical. A cistoscopia confirmou a presença dessa lesão, sendo realizada biópsia. O exame histológico revelou neoplasia de pequenas células. A análise imuno-histoquímica foi necessária para elucidação diagnóstica. O referido exame demonstrou positividade para os marcadores EWS-FLI1, CD99 e S-100. O diagnóstico foi de tumor neuroectodérmico primitivo. A paciente foi tratada com quimioterapia adjuvante. Depois de sete meses de seguimento, a paciente encontra-se livre de doença.The authors report the case of a 52 year-old woman that presented macroscopic hematuria and pelvic pain. ...

Melanocytes distribution and morphology analysis in skin with and without sun exposure

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

CONCLUSION: There is a great variability in the density, distribution and morphology of melanocytes in human skin, mostly in sun-exposed areas. COMMENT: The presence of cytological atypia and cell confluence should not be used as isolated criteria for the definition of a neoplastic lesion (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Phenotypic research on Klebsiella pneumoniae carbapenemase (KPC) enzyme in Enterobacteriaceae from hospitals

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

CONCLUSION: Given the emerging nature of KPC, it is important to trace it in Enterobacteriaceae isolates with decreased susceptibility to ertapenem. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Paracoccidioidomycosis and tuberculosis: differential diagnosis

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

This study reports the case of a patient who allegedly had paracoccidioidomycosis (PCM) for fifteen years. Despite the fact that his baciloscopy result had always been negative, he was empirically treated for tuberculosis twice without clinical improvement. PCM diagnosis through simple methodology draws attention to the importance of parallel investigation into tuberculosis and paracoccidioidomycosis as differential diagnosis in chronic symptomatic patients. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Anthropometric, lipids and blood glucose profiles in adolescents of a philanthropic institution in the northwest Paraná

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

CONCLUSION: The result evidenced the necessity of health public policies in educational scope in order to prevent obesity and its risks factors in adolescence. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

JBPML: crescendo com as investigações na área da Patologia

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Van Gogh: entre a genialidade e a doença

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 21 May 2010 13:53:51 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Classification of journals in the QUALIS system of CAPES: urgent need of changing the criteria

Jornal Brasileiro de Patologia e Medicina Laboratorial — Mon, 17 May 2010 14:00:03 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Utility of tissue microarrays MIB-1 for profiling prognostic biomarkers in clinically localized prostate cancer

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSION: MIB-1 proved to be a prognostic factor comparable to PSA, Gleason and staging.INTRODUÇÃO E OBJETIVO: Analisar a expressão imuno-histoquímica do marcador MIB-1 como fator prognóstico em pacientes com neoplasia de próstata localizada. MÉTODOS: Análise de cem casos de pacientes portadores de neoplasia prostática localizada submetida a cirurgia curativa no período de janeiro de 2000 a dezembro de 2006. Realizou-se o preparo histológico habitual seguido de reação imuno-histoquímica para a detecção do acúmulo da proteína MIB-1 e análise estatística. RESULTADOS: Obteve-se maior proporção de expressão negativa ao marcador MIB-1(63%, p < 0,0001). Os pacientes com expressão positiva (MIB1 positivo) apresentaram antígeno prostático específico (PSA) mais elevado ...

Retrospective study of cutaneous and mucosal melanomas in the population of the state of Rio Grande do Norte, Brazil

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSIONS: The epidemiological profile of the analyzed cases of melanoma was similar to the few published epidemiological studies, although some data on gender and age in certain anatomical sites varied and the incidence of oral melanoma was much lower than that reported in most investigations. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Breast cancer with choriocarcinomatous features

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

This report describes the case of a 45 year-old patient with breast carcinoma characterized by choriocarcinomatous features, presence of bizarre anaplastic cells with areas of necrosis and multinucleated giant cells. The immuno-histochemical analysis showed positivity for chromogranin and β-HCG. The tumor size was 5.7 cm at the largest axis and it was refractory to initial neo-adjuvant treatment. Mastectomy was performed and after microscopic and immuno-histochemical examination it was diagnosed as carcinoma with choriocarcinomatous features. These lesions are rare and liable to synthesize hormonal substances not specific to breast tissue. Its diagnosis is highly significant to the therapeutic and prognostic approach insofar as these lesions are potentially aggressive. (Source: Jornal Bra...

Comparative analysis of the immunohistochemistry expression of p27, p21WAF/Cip1, and p16INK4a in oral normal epithelium, squamous papilloma and squamous cell carcinoma

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSIONS: The study revealed a statistically significant difference for p27 expression when comparing the control and oral squamous papilloma groups with the oral squamous cell carcinoma group. p21WAF/Cip1 did not prove to be useful to differentiate the groups. p16INK4a showed diffuse immunopositivity in a minority of the oral squamous cell carcinoma cases. The oral squamous papilloma group behaved similarly to the control group as to the three markers. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Effect of alloxan-induced diabetes mellitus and ethanol on pregnancy outcome in mice

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSIONS: These results show that, although ethanol might improve energy metabolism in early gestation, it causes cell damage that leads to cardiovascular, limb and neural tube defects, late fetal death and reduced placental size.INTRODUÇÃO E OBJETIVOS: Investigar o efeito do etanol, do diabetes mellitus (DM) e da associação de ambos sobre os fetos de camundongo. MÉTODOS: Foram utilizadas 24 fêmeas de camundongos Swiss divididas em quatro grupos de seis animais cada: controle (C); etanol (E); diabetes (D) (glicemia > 200 mg/dl), e diabetes + etanol (DE). O diabetes foi induzido pela aloxana (40 mg/kg) no dia 7 da gestação. Os animais dos grupos E e DE receberam 4 g/kg de solução a 25% v/v de etanol intraperitoneal (IP), enquanto os animais dos grupos C e D receberam salina. No...

Assessment of environmental conditions in the pathological anatomy laboratory of a university hospital located in Rio de Janeiro

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSION: Biological and chemical agents were identified as the main risks in the work process. Furthermore, the necessity of refurbishment and additional investments in the purchase of individual and collective protection equipment was noticed. The adherence to biosecurity protocols and the introduction of continuing education programs are invaluable to reduce occupational and environmental hazards. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Comparison of hybrid capture and PCR for HPV detection in clinical samples

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSIONS: HC is widely accepted for the detection of HPV, however, it may lack sensitivity in comparison with PCR techniques. RT-PCR has further advantages over the conventional PCR in terms of speed as well as it is slightly more sensitive. Due to their high sensitivity and fast response, PCR methods could be used as a screening method for HPV detection in ectocervical samples. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Urinary tract infections in non hospitalized patients: etiology and antibiotic resistance patterns

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSION: The data reported herein show that the etiology of urinary infections is very similar to those found worldwide. Nonetheless, the resistance pattern of uropathogens may have different characteristics according to the history of antimicrobial consumption in each community. Thus, it is important that epidemiological data are regularly published to aid the medical community. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Quality control in the collection of diagnostic blood specimens: illuminating a dark phase of preanalytical errors

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

CONCLUSIONS: Phlebotomy evaluation is an essential part of the quality planning in clinical laboratories. The proposed checklist allows error detection in the preanalytical phase, establishment of quality indicators and implementation of corrective and preventive actions with cost effectiveness and improvement in process efficiency. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Classificação dos periódicos no sistema QUALIS da CAPES: mudança dos critérios é urgente

Jornal Brasileiro de Patologia e Medicina Laboratorial — Fri, 07 May 2010 14:20:02 +0100

(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Epidemiologic profile of salivary gland neoplasms diagnosed in São Luís-MA

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 23 Jan 2010 14:50:58 +0100

CONCLUSION: In association with other studies, the present results suggest slight variations in the relative frequency and distribution of salivary gland neoplasms between Brazilian and worldwide populations. (Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)

Solid pseudopapillary tumor of the pancreas: report of one uncommon presentation case

Jornal Brasileiro de Patologia e Medicina Laboratorial — Sat, 23 Jan 2010 14:50:58 +0100

Caso clínico atípico de tumor sólido pseudopapilar de pâncreas (TSPPP) acometendo paciente do sexo masculino, 37 anos, com quadro inicial de dor abdominal aguda. Após cirurgia e exame anatomopatológico com imuno-histoquímica, demonstrou padrão condizente com TSPPP e forte positividade para β-catenina. Dois anos após a cirurgia o paciente encontra-se livre de recidiva. O TSPPP é mais comum em mulheres jovens com quadro clínico de massa ou desconforto abdominal, podendo alguns casos ser assintomáticos. Apresenta crescimento lento e baixo potencial de malignidade, sendo a ressecção cirúrgica o tratamento de escolha com altos índices de cura.Atypical clinical case of solid pseudopapillary tumor (SPPT) of the pancreas in a 37 year-old man with acute abdominal pain. After surger...