MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Applied Microbiology' source. Thu, 09 Feb 2012 21:35:02 +0100 http://www.medworm.com/index.php?rid=5674657&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05252.x Conclusion:  Manure lagoons of swine operations imposed both positive and negative selection towards feces‐ and feed‐borne TRG that respectively caused either their proliferation or attenuation in those environments.Significance and Impact of the Study:  The study reveals that discharge of antibiotic resistance genes from swine farms to the environment is linked to their positive selection (defined as an impact leading to proliferation of those genes) in manure lagoons.© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674657 Fri, 10 Feb 2012 01:59:52 +0100 5674657 http://www.medworm.com/index.php?rid=5674663&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05237.x Conclusions:  These data may be important with regard to the spread and persistence of tetracycline resistance genes in the bacterial populations that are present in aquaculture systems.Significance and Impact of the Study:  Interestingly, no isolate has previously been shown to harbour three tet genes that are mediated by efflux systems, but the tetA, tetD and tetE genes were all isolated from one strain, which had the highest MIC value for tetracycline among the strains analysed in this study. We also investigated the full‐length plasmid that encoded the tetE gene from a tetracycline‐resistant strain. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674663 Tue, 07 Feb 2012 05:00:00 +0100 5674663 http://www.medworm.com/index.php?rid=5674660&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05249.x Conclusions:  The use of LAB producing antibacterial substances may be a good choice as a co‐starter culture to ensure the stability of sourdoughs and to avoid the bacterial and fungi spoilage of the end‐product.Significance and Impact of the Study:  The antimicrobial compounds designated as sakacin KTU05‐6, pediocin KTU05‐8 KTU05‐9, KTU05‐10 and AcKTU05‐67 were not identical to any other known BLIS and this finding leads up to the assumption that they might be the novel.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674660 Tue, 07 Feb 2012 05:00:00 +0100 5674660 http://www.medworm.com/index.php?rid=5674659&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05250.x Conclusions:  These studies indicate that anaerobic digestion at 38 or 55°C may be an effective treatment to reduce coliforms and CTC; however, it is not an effective treatment to reduce MON.Significance and Impact of Study:  More studies are needed to determine which pharmaceuticals are susceptible to degradation by a specific manure treatment to prevent negative environmental consequences.© No claim to US Government works. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674659 Tue, 07 Feb 2012 05:00:00 +0100 5674659 http://www.medworm.com/index.php?rid=5674658&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05251.x Conclusions:  A new SAM‐accumulating strain, S. cerevisiae H5MR83, was obtained through spaceflight culture and genetic modification. Under optimal conditions, SAM production was increased to a relative high level in our study.Significance and Impact of the Study:  Through comprehensive application of multiple methods including spaceflight culture, genetic modification, and optimizing cultivation, the yield of SAM could be increased by 6.4 times compared to that in the control strain H5. The obtained S. cerevisiae H5MR83 produced 7.76 g l−1 of SAM in the flask cultures, a significant improvement on previously reported results. The SAM production period with S. cerevisiae H5MR83 was 84 h, which is shorter than previously reported results. S. cerevisiae H5MR83 has considerable potent... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674658 Tue, 07 Feb 2012 05:00:00 +0100 5674658 http://www.medworm.com/index.php?rid=5644302&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05248.x Conclusions:  New insights are thus provided into the interaction mechanisms between Streptomyces sp. and plants; Streptomyces sp. could be sensed by plant cells and through cytosolic Ca2+ changes and the generation of reactive oxygen species, defense responses were induced.Significance and Impact of Study:  These induced defense responses appeared to participate to attenuating Pectobacterium‐induced diseases in plants. Thus Streptomyces sp. OE7 could to be a biocontrol agent against Pectobacterium sp..© No claim to US Government works. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5644302 Tue, 31 Jan 2012 20:38:16 +0100 5644302 http://www.medworm.com/index.php?rid=5644303&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05247.x Conclusions:  Continuous ohmic heating can be effective at killing foodborne pathogens on orange and tomato juice with lower degradation of quality than conventional heating.Significance and Impact of Study:  These results suggest that continuous ohmic heating might be effectively used to pasteurize fruit and vegetable juice in a short operating time and that the effect of inactivation depends on applied electric field strengths, treatment time and electric conductivity.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5644303 Tue, 31 Jan 2012 05:00:00 +0100 5644303 http://www.medworm.com/index.php?rid=5635984&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05246.x Conclusions:  Our present results imply that adenosine was used to survive under extremely acidic conditions via the production of NH3.Significance and Impact of the Study:  It has been proposed that amino acid decarboxylation is the major system for the resistance of E. coli to acidic stress. In the present study, the adenosine deamination was shown to induce the survival under acidic conditions, demonstrating that bacteria have alternative strategies to survive under acidic conditions besides amino acid decarboxylation.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635984 Sat, 28 Jan 2012 02:27:23 +0100 5635984 http://www.medworm.com/index.php?rid=5635986&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05244.x Conclusions:  The analysis identified potential infectious organisms in mastitis, resistance of organisms to antibiotics and chemical compounds, and the natural resistance potential of dairy cows.Significance and Impact of Study: The findings of present study may help in formulating strategies for prevention and treatment of mastitis in dairy animals and consequently in reducing economic losses incurred due to it.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635986 Wed, 25 Jan 2012 05:00:00 +0100 5635986 http://www.medworm.com/index.php?rid=5635985&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05245.x Conclusions:  Some strains with reported resistance to Cu also were also resistant to Ag, suggestive of a shared resistance mechanism such as an indiscriminate Cu efflux pump. E. faecium appears to have innate resistance to both metals. In general, E. faecium was the most resistant species to the individual metals and the combination of metals, P. putida the least resistant, and the Salmonella strains were more resistant than E. coli.Significance and Impact of Study:  Several of the comparison strains with no reported copper resistances were resistant to one or both metals. This may call into question the methods for determining bacterial metal resistance which typically use nutrient‐rich media containing metals to assess the ability of the bacteria to grow in comparison to a wild‐... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635985 Wed, 25 Jan 2012 05:00:00 +0100 5635985 http://www.medworm.com/index.php?rid=5625265&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05243.x Conclusions:  The study highlighted the ability of grapevine ecosystem fungi to degrade biogenic amines and their potential application for biogenic amines removal in wine.Significance and Impact of Study:  The fungi extracts described in this study may be useful in winemaking to reduce the biogenic amines content of wines, thereby preventing the possible adverse effects on health in sensitive individuals and the trade and export of wine.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625265 Wed, 25 Jan 2012 03:53:41 +0100 5625265 http://www.medworm.com/index.php?rid=5625272&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05228.x Conclusions:  The phyllosphere may be a good source of bacteria unrelated or weakly related to human/animal pathogens for screening for new PHA producers for industrial application. Isolate 4D6 was capable of accumulating particularly high levels of PHB from glycerol.Significance and Impact of the Study:  With the increase in biodiesel production, which generates increasing amounts of glycerol as a by‐product, there is a major interest in exploiting this compound as feedstock for the synthesis of interesting products, like biopolymers, such as PHA. The new Massilia sp. 4D6 isolate described in this study may be a useful candidate as a cell factory for the industrial production of PHA from glycerol. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625272 Tue, 24 Jan 2012 05:00:00 +0100 5625272 http://www.medworm.com/index.php?rid=5625271&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05230.x Conclusions:  Since (i) ΔTrelease times were essentially identical in GR‐dependent germination of SD and dormant spores; (ii) rates of GR‐independent germination of SD and dormant spores were identical; (iii) large increases in Tlag times were the major difference in the GR‐dependent germination of SD as compared with spores; and (iv) higher GR levels are correlated with shorter Tlag times, these results are consistent with the hypothesis that low levels of a GR are the major reason that some spores in a population are SD with germinants targeting this same GR.Significance and Impact of the Study:  This study provides information on the dynamic germination of individual SD spores and improves the understanding of spore superdormancy. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625271 Tue, 24 Jan 2012 05:00:00 +0100 5625271 http://www.medworm.com/index.php?rid=5625270&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05239.x Conclusions: Afipia spp. are an appropriate model organism for studying the biodegradation of haloacetic acids in drinking water distribution systems as encoded by dehI genes; the organism that harbors the most prominent dehII gene in drinking water has yet to be cultivated and identified.Significance and Impact of the Study:  The development of a novel application of tRFLP targeting dehI and dehII genes could be broadly useful in understanding haloacetic acid‐degrading bacteria in numerous environments.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625270 Mon, 23 Jan 2012 05:00:00 +0100 5625270 http://www.medworm.com/index.php?rid=5625269&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05240.x AbstractA rapid multiplex PCR method for simultaneous detection of five major foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella Enteritidis, and Shigella flexneri, respectively) was developed. Amplification by PCR was optimized to obtain high efficiency. Sensitivity and specificity assays were investigated by testing different strains. With a multipathogen enrichment, multiplex PCR assay was able to simultaneously detect all of the five organisms in artificially contaminated pork samples. The developed method was further applied to retail meat samples, of which 80% were found to be positive for one or more of these five organisms. All the samples were confirmed by traditional culture methods for each individual species. The result show... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625269 Mon, 23 Jan 2012 05:00:00 +0100 5625269 http://www.medworm.com/index.php?rid=5625268&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05238.x Conclusions:  In B. subtilis the adaptive response to short‐term ethanol stress comprises the recruitment of molecular chaperones on the impaired membrane structure; consequently, the phospholipid synthesis is restored and membrane fluidity adapts properly to the continuing ethanol stress.Significance and Impact of the Study:  These findings underline the role of membrane lipids in establishing tolerance toward ethanol and also suggest the contribution of molecular chaperones to the membrane and cell recovery.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625268 Mon, 23 Jan 2012 05:00:00 +0100 5625268 http://www.medworm.com/index.php?rid=5625267&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05241.x Conclusions:  Nisin F‐loaded brushite cement successfully prevented in vivo growth of S. aureus.Significance and Impact of the Study:  Nisin F incorporated into bone cement may be used to control S. aureus infection in vivo.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625267 Mon, 23 Jan 2012 05:00:00 +0100 5625267 http://www.medworm.com/index.php?rid=5625266&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05242.x Conclusions:  Two novel biosurfactant producers were isolated from petroleum contaminated soil. Biosurfactant from Acinetobacter sp. YC‐X 2 has good properties to a wide range of pH, high temperature and high salinity, and its production was optimized successfully through RSM.Significance and Impact of the study:  The fact, an increasing demand of high quality surfactants and the lack of cost‐competitive bioprocesses of biosurfactants for commercial utilization, motivates researchers to develop cost‐effective strategies for biosurfactant production through isolating new biosurfactant producers with special surface‐active properties and optimizing their cultural conditions. Two novel biosurfactant producers in the present study will widen our knowledge about this kind of microor... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625266 Mon, 23 Jan 2012 05:00:00 +0100 5625266 http://www.medworm.com/index.php?rid=5604516&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05236.x Conclusions:  The organic solvent‐tolerance level in E. coli highly increased by dual disruption of proV and marR.Significance and Impact of the Study:  This study suggests a new strategy for increasing the organic solvent‐tolerance level in E. coli to improve the usability of the whole‐cell biocatalysts in two‐phase systems employing organic solvents.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604516 Thu, 19 Jan 2012 02:50:19 +0100 5604516 http://www.medworm.com/index.php?rid=5644305&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05236.x Conclusions:  The organic solvent tolerance level in E. coli highly increased by dual disruption of proV and marR.Significance and Impact of the Study:  This study suggests a new strategy for increasing the organic solvent tolerance level in E. coli to improve the usability of the whole‐cell biocatalysts in two‐phase systems employing organic solvents. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5644305 Wed, 18 Jan 2012 05:00:00 +0100 5644305 http://www.medworm.com/index.php?rid=5604517&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05235.x Conclusions:  The qmPCR assays developed in this study provide reliable and simultaneous detection and quantification of C. jejuni and C. coli, with good amplification reaction parameters.Significance and Impact of Study:  Following further validation, the qmPCR assay reported here has the potential to be applied to various sample types as an alternative and rapid methodology.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604517 Wed, 18 Jan 2012 05:00:00 +0100 5604517 http://www.medworm.com/index.php?rid=5594038&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05227.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5594038 Thu, 12 Jan 2012 05:00:00 +0100 5594038 http://www.medworm.com/index.php?rid=5594039&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05226.x Conclusions: Lactobacillus brevis [C11] produced acidic metabolites that were able to inhibit the growth of wood decay fungi and subsequent wood decay.Significance and Impact of the Study:  Traditional wood treatments are becoming an environmental issue as the public demands more benign options. The use of lactic acid bacteria which are considered safe for general use is a potential alternative to the conventional heavy metal chemicals currently in use. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5594039 Wed, 11 Jan 2012 05:00:00 +0100 5594039 http://www.medworm.com/index.php?rid=5644304&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05234.x Conclusions:  Our results suggest that infection risks are mainly dependent on current infectious doses of pathogens. For enteritic viruses or bacteria, for example Norovirus or EHEC, only a few particles or cells are sufficient for infection in public lavatories, thus bearing a high risk of infection for other persons. However, there seems to be only a low probability of becoming infected with pathogens that have a high infectious dose whilst sharing the same bathroom.Significance and Impact of the Study:  The transmission model for micro‐organisms enables a risk assessment of gastro‐intestinal infections on the basis of a practical approach. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5644304 Mon, 09 Jan 2012 05:00:00 +0100 5644304 http://www.medworm.com/index.php?rid=5577118&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05233.x Conclusions:  Clinical isolates of MSRP isolated from dogs in North China belonged to two major clonal lineages ST71 and ST5.Significance and Impact of the Study:  This study is the first report on MRSP from canine pyoderma in China. Further surveillance study is needed to gain more detailed data concerning this major clinical challenge in veterinary medicine.© 2012The Authors Journal of Applied Microbiology© 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5577118 Mon, 09 Jan 2012 05:00:00 +0100 5577118 http://www.medworm.com/index.php?rid=5577119&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05232.x Conclusions:  We have successfully developed the cell‐surface display of xylanase on E. coli. The cell‐surface display can enhance the stability of xylanase against changes in temperature, and has the potential of becoming a whole‐cell biocatalyst for industrial applications, such as biobleaching of paper and production of renewable energy.Significance and Impact of the Study:  The results demonstrated that the cell‐surface display of xylanase embedded in the cell membrane is more stable than that of the purified enzyme. Thus, to improve the stability of heterologous proteins production, cell‐surface display using the PgsA anchor protein as a tool can be considered in E. coli.© 2012The Authors Journal of Applied Microbiology© 2012 The Society for Applied Microbiology (Sourc... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5577119 Sat, 07 Jan 2012 05:00:00 +0100 5577119 http://www.medworm.com/index.php?rid=5577117&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05234.x Conclusions:  Our results suggest that infection risks are mainly dependent on current infectious doses of pathogens. For enteritic viruses or bacteria, e.g. Norovirus or EHEC, only a few particles or cells are sufficient for infection in public lavatories, thus bearing a high risk of infection for other persons. However, there seems to be only a low probability of becoming infected with pathogens that have a high infectious dose whilst sharing the same bathroom.Significance and Impact of the Study: The transmission model for microorganisms enables a risk assessment of gastro‐intestinal infections on the basis of a practical approach.© 2012The Authors Journal of Applied Microbiology© 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5577117 Sun, 01 Jan 2012 05:00:00 +0100 5577117 http://www.medworm.com/index.php?rid=5569711&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2012.05231.x Conclusions:  Based on the partial capsid gene sequences, we identified a great number of NoV strains belonging to many genotypes, demonstrating that genetically diverse NoV strains are co‐circulating in aquatic environments and human populations.Significance and Impact of Study:  Our results clearly demonstrate the seasonal trend and genetic diversity of NoVs in wastewater.© 2012The Authors Journal of Applied Microbiology© 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569711 Sun, 01 Jan 2012 05:00:00 +0100 5569711 http://www.medworm.com/index.php?rid=5550288&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05230.x Conclusions:  Since: i) ΔTrelease times were essentially identical in GR‐dependent germination of SD and dormant spores; ii) rates of GR‐independent germination of SD and dormant spores were identical; iii) large increases in Tlag times were the major difference in the GR‐dependent germination of SD as compared with spores; and iv) higher GR levels are correlated with shorter Tlag times, these results are consistent with the hypothesis that low levels of a GR are the major reason that some spores in a population are SD with germinants targeting this same GR.Significance and Impact of the Study:  This study provides information on the dynamic germination of individual SD spores and improves the understanding of spore superdormancy.© 2011 The Authors Journal of Applied Microbiolo... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5550288 Thu, 29 Dec 2011 22:11:52 +0100 5550288 http://www.medworm.com/index.php?rid=5625274&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05230.x Conclusions:  Since (i) ΔTrelease times were essentially identical in GR‐dependent germination of SD and dormant spores; (ii) rates of GR‐independent germination of SD and dormant spores were identical; (iii) large increases in Tlag times were the major difference in the GR‐dependent germination of SD as compared with spores; and (iv) higher GR levels are correlated with shorter Tlag times, these results are consistent with the hypothesis that low levels of a GR are the major reason that some spores in a population are SD with germinants targeting this same GR.Significance and Impact of the Study:  This study provides information on the dynamic germination of individual SD spores and improves the understanding of spore superdormancy. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625274 Wed, 28 Dec 2011 05:00:00 +0100 5625274 http://www.medworm.com/index.php?rid=5550290&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05228.x Conclusions:  The phyllosphere may be a good source of bacteria unrelated or weakly related to human/animal pathogens for screening for new PHA producers for industrial application. Isolate 4D6 was capable of accumulating particularly high levels of PHB from glycerol.Significance and Impact of the Study:  With the increase in biodiesel production, which generates increasing amounts of glycerol as a by‐product, there is a major interest in exploiting this compound as feedstock for synthesis of interesting products, like biopolymers, such as PHA. The new Massilia sp. 4D6 isolate described in this study may be a useful candidate as a cell factory for the industrial production of PHA from glycerol.© 2011 The Authors Journal of Applied Microbiology © 2011 The Society for Applied Microbi... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5550290 Wed, 28 Dec 2011 05:00:00 +0100 5550290 http://www.medworm.com/index.php?rid=5550289&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05229.x Conclusions:  This study demonstrates year round detection of V. vulnificus while likely in the viable but non‐culturable (VBNC) state during the winter months and emphasizes why physicochemical factors are insufficient metrics for robust regression modeling of this pathogen.Significance and Impact of the Study: This study provides an effective new tool, Q‐PCR, to study environmental distribution of V. vulnificus and that in light of the patchy distribution observed, new reliable approaches and a mechanistic understanding of pathogen ecology need to be considered to effectively model the aquatic distribution of V. vulnificus.© 2011 The Authors Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5550289 Wed, 28 Dec 2011 05:00:00 +0100 5550289 http://www.medworm.com/index.php?rid=5534269&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05226.x Conclusions: Lactobacillus brevis [C11] produced acidic metabolites which were able to inhibit growth of wood decay fungi and subsequent wood decay.Significance and Impact of the Study:  Traditional wood treatments are becoming an environmental issue as the public demands more benign options. The use of lactic acid bacteria which are considered safe for general use is a potential alternative to the conventional heavy metal chemicals currently in use.© 2011 Scion Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534269 Fri, 23 Dec 2011 15:07:36 +0100 5534269 http://www.medworm.com/index.php?rid=5625273&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05224.x Conclusions:  A response to a low‐energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage‐related proteins, probably to protect the bacterium against a new PL‐induced stress.Significance and Impact of the Study:  Taken together, these results suggest that a low‐energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625273 Wed, 21 Dec 2011 05:00:00 +0100 5625273 http://www.medworm.com/index.php?rid=5534275&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05221.x Conclusion:  Not all Campylobacter species encode luxS. Food matrices can reduce AI‐2 activity in a LuxS independent manner.Significance and impact of the study:  Beside C. lari, C. peloridis and C. insulaenigrae do not show AI‐2 activity. Further investigations should clarify the function of AI‐2 in Campylobacter spp. and how species lacking luxS could overcome this alteration. Furthermore the impact of food matrices on these functions needs to be determined as we could show that chicken juice reduced AI‐2 activity.© 2011The Authors Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534275 Wed, 21 Dec 2011 05:00:00 +0100 5534275 http://www.medworm.com/index.php?rid=5534274&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05222.x Conclusions:  The antimicrobial activity of lemongrass oil against S. Newport was concentration and time dependent. The antimicrobial activity increased with exposure time; iceberg samples treated for 2 min generally showed greater reductions (p<0.05) than those treated for 1 min (ca. 1‐log reduction difference for 0.3% and 0.5% treatments). Few samples showed a difference between refrigeration and abuse temperatures.Significance:  This study demonstrates the potential of lemongrass oil solutions to inactivate S. Newport on organic leafy greens.© 2011 © University of Arizona Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534274 Wed, 21 Dec 2011 05:00:00 +0100 5534274 http://www.medworm.com/index.php?rid=5534273&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05223.x Conclusions:  For starting surface temperatures below the dew‐point‐temperature of the sterilant gas the condensation of highly concentrated liquid H2O2 on the packaging surface accelerates the killing the spores, while the inferior wettability of more hydrophobic spores compared to more hydrophilic ones diminishes the effect.Impact of the study:  Regarding industrial packaging sterilization, a mixed microflora has to be inactivated. Promoting the condensation of H2O2 improves in general the killing of different species of spores, however at various degrees depending on the wettability of spores.© 2011 © Technische Universität München Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534273 Wed, 21 Dec 2011 05:00:00 +0100 5534273 http://www.medworm.com/index.php?rid=5534271&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05224.x Conclusions:  A response to a low‐energy PL dose takes place in P. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes in order to produce proteins involved in chaperone mechanisms and phage‐related proteins, probably to protect the bacterium against a new PL induced stress.Significance and impact of Study:  Taken together, these results suggest that a low‐energy PL dose is sufficient to provoke adaptation of P. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.© No claim to French Government works. Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534271 Wed, 21 Dec 2011 05:00:00 +0100 5534271 http://www.medworm.com/index.php?rid=5534270&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05225.x Conclusions:  Quantities of cld genes and transcripts per μg of DNA and RNA, respectively, were found to be higher when perchlorate removal was higher. However, quantities of pcrA and cld genes or transcripts were not found to directly correlate with perchlorate removal rates.Significance and Impact of Study:  To our knowledge, this study represents the first application of qPCR assays to quantify perchlorate‐related genes and transcripts in continuous‐flow bioreactors. The results indicate that cld gene and transcript quantities can provide insights regarding the quantity, location, and gene expression of PRB in bioreactors.© 2011The Authors Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534270 Wed, 21 Dec 2011 05:00:00 +0100 5534270 http://www.medworm.com/index.php?rid=5520941&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05220.x Conclusions:  The compatible microbial consortia triggered defense responses in an enhanced level in pea than the microbes alone and provided better protection against Sclerotinia rot.Significance and Impact of the Study:  Rhizosphere microbes in consortium can enhance protection in pea against the soft rot pathogen through augmented elicitation of host defense responses.© 2011The Authors Journal of Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5520941 Tue, 20 Dec 2011 14:09:41 +0100 5520941 http://www.medworm.com/index.php?rid=5594037&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05220.x Conclusions:  The compatible microbial consortia triggered defence responses in an enhanced level in pea than the microbes alone and provided better protection against Sclerotinia rot.Significance and Impact of the Study:  Rhizosphere microbes in consortium can enhance protection in pea against the soft‐rot pathogen through augmented elicitation of host defence responses. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5594037 Mon, 19 Dec 2011 05:00:00 +0100 5594037 http://www.medworm.com/index.php?rid=5569712&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05218.x Conclusions: Cronobacter sakazakii resistance to thermal and nonthermal preservation technologies can increase or decrease as a consequence of previous exposure to stressing conditions.Significance and Impact of the Study:  The results help in understanding the physiology of the resistance of this emerging pathogen to traditional and novel preservation technologies. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569712 Mon, 19 Dec 2011 05:00:00 +0100 5569712 http://www.medworm.com/index.php?rid=5520943&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05218.x Conclusions: C. sakazakii resistance to thermal and non‐thermal preservation technologies can increase or decrease as a consequence of previous exposure to stressing conditions.Significance and Impact of the Study:  The results help in understanding the physiology of the resistance of this emerging pathogen to traditional and novel preservation technologies. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5520943 Mon, 19 Dec 2011 05:00:00 +0100 5520943 http://www.medworm.com/index.php?rid=5520942&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05219.x Conclusions:  SIP18p acts as an inhibitor of apoptosis in yeast under dehydration stress, as suggested by its antioxidative capacity through the ROS accumulation reduction after an H2O2 attack.Significance and Impact of Study:  To our knowledge, this is the first systematic screen for identification of putative genes essential to overcoming cell dehydration process. A broad range of identified genes could help to understand why some strains of high biotechnological interest cannot cope with the drying and rehydration treatment. Dehydration sensitivity makes these strains not suitable to be commercialised by yeast manufactures.Published 2011. This article is a US Government work and is in the public domain in the USA (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5520942 Mon, 19 Dec 2011 05:00:00 +0100 5520942 http://www.medworm.com/index.php?rid=5501755&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05217.x Conclusions:  ZVI biosand filters were more effective in reducing E. coli O157:H12 populations in irrigation water than sand filters.Significance and Impact of Study:  Zero‐valent ion treatment may be a cost‐effective mitigation step to help small farmers reduce risk of foodborne E. coli infections associated with contamination of leafy greens. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501755 Wed, 14 Dec 2011 16:07:48 +0100 5501755 http://www.medworm.com/index.php?rid=5594040&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05217.x Conclusions:  ZVI biosand filters were more effective in reducing E. coli O157:H12 populations in irrigation water than sand filters.Significance and Impact of the Study:  Zero‐valent ion treatment may be a cost‐effective mitigation step to help small farmers reduce risk of foodborne E. coli infections associated with contamination of leafy greens. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5594040 Mon, 12 Dec 2011 05:00:00 +0100 5594040 http://www.medworm.com/index.php?rid=5501757&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05215.x Conclusions:  These results demonstrated the effectiveness of rapid cell collection and FT‐IR spectroscopy techniques to differentiate between live and dead E. coli O157:H7 cells.Significance and Impact of the Study:  This technique has potential applications for use with foods subjected to various inactivation treatments. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501757 Mon, 12 Dec 2011 05:00:00 +0100 5501757 http://www.medworm.com/index.php?rid=5501756&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05216.x Conclusions:  Addition of heat to the compressed CO2 treatment of textiles inoculated with microorganisms proved more effective than the addition of detergent or disinfectant with compressed CO2 treatment at temperature of 20°C.Significance and Impact of the Study:  CO2 treatment of textiles is a promising ecological alternative dry cleaning method for the disinfection of medical textiles. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501756 Mon, 12 Dec 2011 05:00:00 +0100 5501756 http://www.medworm.com/index.php?rid=5577120&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05214.x Conclusions:  This method is suitable not only for the expression of GFP in P. larvae but also for the expression of heterologous proteins or GFP‐tagged proteins in P. larvae. Mutants can be used for infection assays because GFP expression remained stable after sporulation and re‐germination.Significance and Impact of the Study:  This method provides the first true molecular tool for P. larvae and, therefore, is an immense advancement from what we had previously at our hands for the study of P. larvae pathogenesis. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5577120 Fri, 09 Dec 2011 05:00:00 +0100 5577120 http://www.medworm.com/index.php?rid=5493028&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05201.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5493028 Fri, 09 Dec 2011 05:00:00 +0100 5493028 http://www.medworm.com/index.php?rid=5483933&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05212.x Conclusions:  The analysis of nucleotide sequence variations within the serB gene could serve as a suitable tool for probing diverse genotypes of S. thermophilus. S. thermophilus isolates associated with traditional Egyptian dairy products show high degree of genetic and phenotypic diversity.Significance and Impact of Study:  This study presents a novel, simplified procedure based on serB nucleotide sequencing for genotyping S. thermophilus. It also provides a pool of phenotypically diverse S. thermophilus cultures, from which certain strains could be selected for use in bioprocessing applications including fermented dairy products. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5483933 Mon, 05 Dec 2011 05:00:00 +0100 5483933 http://www.medworm.com/index.php?rid=5493027&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05214.x Conclusions:  This method is suitable not only for the expression of GFP in P. larvae but also for the expression of heterologous proteins or GFP‐tagged proteins in P. larvae. Mutants can be used for infection assays since GFP expression remained stable after sporulation and re‐germination.Significance and Impact of the Study:  This method provides the first true molecular tool for P. larvae and, therefore, is an immense advancement from what we had previously at our hands for the study of P. larvae pathogenesis. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5493027 Thu, 01 Dec 2011 05:00:00 +0100 5493027 http://www.medworm.com/index.php?rid=5483932&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05213.x Conclusions:  The combined analysis of 16S rRNA and bamA genes suggests the species related to genera Thauera dominated toluene and benzoate degrading cultures. The combination of multiple methods (DGGE and cloning) provides a more complete picture of bamA gene diversity.Significance and impact of the study:  To our knowledge this is the first report of bamA gene in denitrifying enrichments using DGGE and cloning analysis. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5483932 Thu, 01 Dec 2011 05:00:00 +0100 5483932 http://www.medworm.com/index.php?rid=5465778&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05210.x Conclusions:  The calibration and validation of methods based on multivariate analysis allowed the definition of models for the quantification of Pb+2, Cd+2 and Ni+2 attached to bacterial surfaces. The high percentages of explained variances in PCA gave a strong support to calibrate the prediction models, depicting very good correlations with the reference method (correlations ∼ 0.90 in all cases).Significance and Impact of the study: L. kefir CIDCA 8348 and JCM 5818 bind Pb+2, Cd+2 and Ni+2 in an efficient way. This fact gives support for their potential use as sequestrants of traces of these metals in products addressed to human and animal consume. The prediction models developed would be useful for the determination of the investigated metal ions in unknown samples giving at the s... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465778 Thu, 01 Dec 2011 05:00:00 +0100 5465778 http://www.medworm.com/index.php?rid=5454454&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05205.x Conclusions:  Phenazines and specific lipopeptides play an important role in suppression of stem rot disease of groundnut by root‐colonizing Pseudomonas strains.Significance and Impact of the Study: Pseudomonas strains Phz24 and SH‐C52 showed significant control of stem rot disease. Treatment of seeds or soil with these strains provides a promising supplementary strategy to control stem rot disease of groundnut. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5454454 Tue, 29 Nov 2011 15:02:15 +0100 5454454 http://www.medworm.com/index.php?rid=5569716&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05207.x Conclusions:  The shelf life was 7 days for control samples at 5°C. The osmotic pretreatment with carvacrol, glucono‐δ‐lactone and Citrox allowed for shelf‐life extension by 8, 10 and 5 days at 5°C, respectively.Significance and Impact of the Study:  The results of the study show the potential of adding carvacrol, glucono‐δ‐lactone or Citrox in the osmotic solution to extend the shelf life and improve commercial value of chilled osmotically pretreated fish products. The developed models can be a reliable tool for predicting the shelf life of fresh or minimally processed gilthead seabream fillets in the real chill chain. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569716 Tue, 29 Nov 2011 05:00:00 +0100 5569716 http://www.medworm.com/index.php?rid=5569715&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05209.x Conclusions:  A genetically stable mutant strain with high ethanol yield under heat stress was obtained using HEPE. This mutant may be a suitable candidate for the industrial‐scale ethanol production.Significance and Impact of the Study:  High‐energy‐pulse‐electron radiation is a new efficient technology in breeding micro‐organisms. The mutant obtained in this work has the advantages in industrial ethanol production under thermostress. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569715 Tue, 29 Nov 2011 05:00:00 +0100 5569715 http://www.medworm.com/index.php?rid=5569714&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05208.x Conclusions:  The isolate CS3 showed outstanding nicotine‐degrading characteristics such as high degradation efficiency, strong substrate endurance, broad pH adaptability, and stability and persistence in repetitive degradation processes and may serve as an excellent candidate for applications in the bioaugmentation process to treat nicotine‐contaminated water and soil. Also, detection of nicotine metabolites suggests that strain CS3 might decompose nicotine via a unique nicotine‐degradation pathway.Significance and Impact of the Study:  The advantage of applying the isolated strain lies in broad pH adaptability and stability and persistence in repetitive use, the properties previously less focused in other nicotine‐degrading micro‐organisms. The strain might decompose nicoti... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569714 Tue, 29 Nov 2011 05:00:00 +0100 5569714 http://www.medworm.com/index.php?rid=5534280&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05211.x Conclusions:  Both sponges possess a diverse range of bioactive and potentially novel bacteria. Differences observed from the sponge‐derived groups of isolates in terms of bioactivity suggest that S. carnosus isolates may be a better source of antibacterial compounds, while Leucosolenia sp. isolates appear to be a better source of antifungal compounds.Significance and Impact of the Study:  This is the first study in which cultured bacterial isolates from the marine sponges S. carnosus and a Leucosolenia sp. have been evaluated for their antibacterial activity. The high percentage of antibacterial isolates from S. carnosus and of antifungal isolates from Leucosolenia sp. suggests that these two sponges may be good sources for potentially novel marine natural products. (Source: Jour... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534280 Tue, 29 Nov 2011 05:00:00 +0100 5534280 http://www.medworm.com/index.php?rid=5465783&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05206.x Conclusions:  The results confirmed the feasibility that a “universal targeting vector” could be constructed through Red/ET recombination by using the conserved regions of KLM gene clusters to detect ansamycins production in actinomycetes.Significance and Impact of the Study:  The “universal targeting vector” provides a rapid approach in certain degree to detect the potential ansamycin producers from the 22 KLM gene cluster positive actinomycetes strains. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465783 Tue, 29 Nov 2011 05:00:00 +0100 5465783 http://www.medworm.com/index.php?rid=5465782&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05207.x Conclusions:  The shelf life was 7 d for control samples at 5°C. The osmotic pre‐treatment with carvacrol, glucono‐δ‐lactone and Citrox allowed for shelf life extension by 8, 10 and 5 d at 5°C, respectively.Significance and Impact of Study:  The results of the study show the potential of adding carvacrol, glucono‐δ‐lactone or Citrox in the osmotic solution to extend the shelf life and improve commercial value of chilled osmotically pre‐treated fish products. The developed models can be a reliable tool for predicting the shelf life of fresh or minimally processed gilthead seabream fillets in the real chill chain. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465782 Tue, 29 Nov 2011 05:00:00 +0100 5465782 http://www.medworm.com/index.php?rid=5465781&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05208.x Conclusions:  The isolate CS3 showed outstanding nicotine‐degrading characteristics such as high degradation efficiency, strong substrate endurance, broad pH adaptability, and stability and persistence in repetitive degradation processes, and may serve as an excellent candidate for applications in the bioaugmentation process to treat nicotine‐contaminated water and soil. Also, detect of nicotine metabolites suggests that strain CS3 might decompose nicotine via a unique nicotine‐degradation pathway.Significance and Impact of the Study:  The advantage of applying the isolated strain lies in broad pH adaptability and stability and persistence in repetitive use, the properties previously less focused on in other nicotine‐degrading microorganisms. The strain might decompose nicotine... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465781 Tue, 29 Nov 2011 05:00:00 +0100 5465781 http://www.medworm.com/index.php?rid=5465780&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05209.x Conclusions:  A genetically stable mutant strain with high ethanol yield under heat stress was obtained using HEPE. This mutant may be a suitable candidate for the industrial‐scale ethanol production.Significance and Impact of the Study:  HEPE radiation is a new efficient technology in breeding of micro‐organisms. The mutant obtained in this work has the advantages in ethanol production industrial under thermo stress. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465780 Tue, 29 Nov 2011 05:00:00 +0100 5465780 http://www.medworm.com/index.php?rid=5465779&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05211.x Conclusions:  Both sponges possess a diverse range of bioactive and potentially novel bacteria. Differences observed from the sponge‐derived groups of isolates in terms of bioactivity suggests that S. carnosus isolates may be a better source of antibacterial compounds, while Leucosolenia sp. isolates appear to be a better source of antifungal compounds.Significance and Impact of the study:  This is the first study in which cultured bacterial isolates from the marine sponges S. carnosus and a Leucosolenia sp. have been evaluated for their antibacterial activity. The high percentage of antibacterial isolates from S. carnosus and of antifungal isolates from Leucosolenia sp. suggests that these two sponges may be good sources for potentially novel marine natural products. (Source: Journa... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465779 Tue, 29 Nov 2011 05:00:00 +0100 5465779 http://www.medworm.com/index.php?rid=5534281&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05204.x SummarySpecies of Bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. These organisms have undergone huge taxonomic changes in the last 30 years, with numbers of genera and species now standing at 56 and over 545, respectively. Despite this expansion, relatively few new species have been isolated from infections, few are associated with food and no important new agents of foodborne illness have been reported. What has changed is our knowledge of the established agents. Bacillus cereus is well known as a cause of food poisoning, and much more is now understood about its toxins and their involvement in infections and intoxications. Also, although B. licheniformis, B. subtilis and B. pumilus have occasionally been isolated fr... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534281 Mon, 28 Nov 2011 05:00:00 +0100 5534281 http://www.medworm.com/index.php?rid=5454455&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05204.x SummarySpecies of Bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. These organisms have undergone huge taxonomic changes in the last 30 years, with numbers of genera and species now standing at 56 and over 545 respectively. Despite this expansion, relatively few new species have been isolated from infections, few are associated with food, and no important new agents of food‐borne illness have been reported. What has changed is our knowledge of the established agents. Bacillus cereus is well known as a cause of food poisoning, and much more is now understood about its toxins and their involvement in infections and intoxications. Also, although B. licheniformis, B. subtilis and B. pumilus have occasionally been isolated from... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5454455 Mon, 28 Nov 2011 05:00:00 +0100 5454455 http://www.medworm.com/index.php?rid=5447965&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05203.x Conclusions:  The absolute risk of incursion of CCHFV in livestock through ticks introduced by four abundant species of migratory bird (totalling 120 million individual birds) is very low. Climate change has opposing effects; increasing the success of the moult of the nymphal ticks into adults but decreasing the projected abundance of birds by 34% in this model.Significance and Impact of the Study:  For Europe, climate change is not predicted to increase the overall risk of incursion of CCHFV in livestock through infected ticks introduced by these four migratory bird species. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447965 Sat, 26 Nov 2011 15:41:00 +0100 5447965 http://www.medworm.com/index.php?rid=5447966&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05202.x Conclusions:  Organic farming management was more beneficial in terms of diversity and abundance than the conventional one. Induced fermentation generated a great replacement of native yeasts. Although winery‐resident yeasts resulted to be predominant in the process, some non‐commercial strains originally in the vineyard were found in final stages of the fermentation, confirming that autochthonous strains of S. cerevisiae are capable to conduct the fermentation process up to its end.Significance and Impact of the study:  The study of natural yeast communities from commercial vineyards and wineries is an important step towards the preservation of native genetic resources. Our results have special relevance since it is the first time that the real situation of the yeast ecology of al... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447966 Sat, 26 Nov 2011 05:00:00 +0100 5447966 http://www.medworm.com/index.php?rid=5428641&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05200.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428641 Sun, 20 Nov 2011 14:45:39 +0100 5428641 http://www.medworm.com/index.php?rid=5418545&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05197.x Conclusions:  Removal of bacteria and fungi was best accomplished using a mixture of 3 antibiotics and 1 antifungal; rifampicin (300 mg l−1), streptomycin/penicillin (25 mg l−1) and nystatin (10 mg l−1) incorporated in seawater samples and incorporated into cultures every 24 hours for a minimum of two days.Significance and Impact of the Study:  The axenic isolation and culture of marine thraustochytrids from a marine habitat in New Zealand has significant implications for the biotechnological development of these potentially valuable protists. This method has global significance as it is reasonable to assume it could be used throughout the world to obtain axenic thraustochytrid cultures. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418545 Fri, 18 Nov 2011 05:05:46 +0100 5418545 http://www.medworm.com/index.php?rid=5569713&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05200.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569713 Fri, 18 Nov 2011 05:00:00 +0100 5569713 http://www.medworm.com/index.php?rid=5428643&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05198.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428643 Fri, 18 Nov 2011 05:00:00 +0100 5428643 http://www.medworm.com/index.php?rid=5428642&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05199.x Conclusions:  AHL production was observed in the majority of the studied species, with a diverse biosensor activation profile.Significance and Impact of the Study:  The high diversity in AHL production is in consistence with the high diversity in ecological niches of the Vibrionaceae family. The absence of AHL detection in 8 species warrants further work on their quorum sensing systems. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428642 Fri, 18 Nov 2011 05:00:00 +0100 5428642 http://www.medworm.com/index.php?rid=5418548&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05194.x Conclusion:  The combined application of the N‐acyl HSL analog and antibiotics was effective at reducing the viability of P. gingivalis cells in biofilms.Significance and Impact of Study:  The combined application of the N‐acyl HSL analog and antibiotics may be successful for eradicating infections involving bacterial biofilms, such as periodontitis. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418548 Wed, 16 Nov 2011 05:00:00 +0100 5418548 http://www.medworm.com/index.php?rid=5418547&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05195.x Conclusions:  Physico‐chemical surface properties of 18 strains determined show large variations between the strains. However, no direct link between these surface properties and the resistant/sensitive phenotypes of the strains can be stated.Significance and impact of the study:  The surface physico‐chemical properties tested have a low predictive power to discriminate sensitive or resistant strains when determined at the bacterial population scale. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418547 Wed, 16 Nov 2011 05:00:00 +0100 5418547 http://www.medworm.com/index.php?rid=5418546&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05196.x Conclusions:  Lenticulation was shown to be an effective means of preservation of detectable noroviruses. LENTICULES™ were sufficiency homogeneous and stable throughout medium term frozen and short term storage at room temperature to serve as RMs. Virus LENTICULES™ have the advantages of being easy to manipulate, provide assigned values and do not require the manipulation of high titre clinical material.Significance and impact of the study:  The results of this study show that norovirus LENTICULES™ can be used as stable RMs for quantitative real‐time RT‐PCR assays. They can be utilised as in‐run positive extraction controls and potentially for method calibration, and to enable more easy comparison of data generated by the variety of differing norovirus determination methods... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418546 Wed, 16 Nov 2011 05:00:00 +0100 5418546 http://www.medworm.com/index.php?rid=5454456&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05192.x Conclusions: Nosema ceranae and N. apis are not tissue specific, and honeybee glands have potential to become a useful indicator of the extent of disease in the colony and may represent a potential infection reservoir.Significance and Impact of the Study:  First report of spore load quantification of Nosema spp. in different honeybee glands. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5454456 Sun, 06 Nov 2011 04:00:00 +0100 5454456 http://www.medworm.com/index.php?rid=5447967&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05189.x Conclusions: Burkholderia sp. USM (JCM15050) has the metabolic pathways to supply both the short‐chain length (SCL) and medium‐chain length (MCL) PHA monomers. By transforming the strain with the Aer. caviae PHA synthase with broader substrate specificity, SCL‐MCL PHA was produced.Significance and Impact of the Study:  This is the first study demonstrating the ability of transformant Burkholderia to produce P(3HB‐co‐3HHx) from a single carbon source. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447967 Sun, 06 Nov 2011 04:00:00 +0100 5447967 http://www.medworm.com/index.php?rid=5397540&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05189.x Conclusions: Burkholderia sp. USM (JCM15050) has the metabolic pathways to supply both the short‐chain length (SCL) and medium‐chain length (MCL) PHA monomers. By transforming the strain with the A. caviae PHA synthase with broader substrate specificity, SCL‐MCL PHA was produced.Significance and Impact of Study: This is the first study demonstrating the ability of transformant Burkholderia to produce P(3HB‐co‐3HHx) from a single carbon source. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397540 Sun, 06 Nov 2011 04:00:00 +0100 5397540 http://www.medworm.com/index.php?rid=5397539&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05190.x Conclusion:  These results indicated that these flavonoids have the potential to reverse bacterial resistance to amoxicillin in AREC and may operate via three mechanisms: inhibition of peptidoglycan and ribosome synthesis, alteration of outer membrane permeability and interaction with β‐lactamases.Significance and Impact of the Study:  These findings offer the potential to develop a new generation of phytopharmaceuticals to treat AREC. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397539 Sun, 06 Nov 2011 04:00:00 +0100 5397539 http://www.medworm.com/index.php?rid=5397538&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05191.x Conclusion:  Environmental parameters played a significant role in the emergence and spread of cholera and the abundance of V. cholerae. But, based on virulence gene profiling and genetic fingerprinting the possibility of origin of toxigenic isolates from non‐toxigenic environmental isolates seems unlikely in freshwater environs of India.Significance of the study:  Explains the ecology, epidemiology and seasonality of cholera in freshwater environs. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397538 Sun, 06 Nov 2011 04:00:00 +0100 5397538 http://www.medworm.com/index.php?rid=5397537&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05192.x Conclusions: N. ceranae and N. apis are not tissue specific, and honeybee glands have potential to become a useful indicator of the extent of disease in the colony and may represent a potential infection reservoir.Significance and Impact of the Study: First report of spore load quantification of Nosema spp. in different honeybee glands. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397537 Sun, 06 Nov 2011 04:00:00 +0100 5397537 http://www.medworm.com/index.php?rid=5447968&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05188.x Conclusions:  The overexpression of the ABC transporter caused by the point mutations at the binding sites was responsible for the fungal resistance to various pesticides including carbendazim.Significance and Impact of Study:  The transporter‐mediated multidrug resistance found for the first time in entomopathogenic fungi is potential for use in improving mycoinsecticide compatibility with chemical pesticides. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447968 Wed, 02 Nov 2011 04:00:00 +0100 5447968 http://www.medworm.com/index.php?rid=5376551&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05187.x Conclusions:  Our results suggested that different environmental nutrient inputs contribute to seasonal and temporal variations of chemical features and bacterial communities in sediments of Lake Dongping. Total phosphorus concentration was significantly correlated with the distribution of bacterial communities.Significance and impact of the Study:  This study has an important implication for optimization of integrated ecosystem assessment of shallow temperate freshwater lake, and provides interesting information for the subsequent of the ecosystem. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376551 Wed, 02 Nov 2011 04:00:00 +0100 5376551 http://www.medworm.com/index.php?rid=5397536&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05193.x SummaryThe potential for transmission of antibiotic‐resistant enteric zoonotic bacteria from animals to humans has been a public health concern for several decades. Bacteria carrying antibiotic resistance genes found in the intestinal tract of food animals can contaminate carcasses and may lead to food‐borne disease in humans that may not respond to antibiotic treatment. It is consequently important to monitor changes in antimicrobial susceptibility of zoonotic and commensal organism; in this context there are a number of veterinary monitoring programmes that collect bacteria in food‐producing animals at slaughter and determine their susceptibility against antibiotics relevant for human medicine. The data generated are part of the risk analysis for potential food‐borne transmission... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397536 Tue, 01 Nov 2011 04:00:00 +0100 5397536 http://www.medworm.com/index.php?rid=5376550&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05188.x Conclusions:  The overexpression of the ABC transporter caused by the point mutations at the binding sites was responsible for the fungal resistance to various pesticides including carbendazim.Significance and Impact of Study:  The transporter‐mediated multidrug resistance found for the first time in entomopathogenic fungi is potential for use in improving mycoinsecticide compatibility with chemical pesticides. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376550 Tue, 01 Nov 2011 04:00:00 +0100 5376550 http://www.medworm.com/index.php?rid=5437727&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05185.x Conclusions:  This confirms that MF staining does not only record permanently the morphological changes and retain internal structural details but also provides a reliable and rapid viability assay for the parasites.Significance and Impact of the Study:  Therefore, in our study, Modified Field’s stain may offer the researchers and laboratory technologists the opportunity to get the result on the same day and the most important thing is the ability to differentiate between viable and non‐viable of T. vaginalis under three different conditions (normal culture, drug and distilled water condition). Modified Field’s staining method enhanced the morphological identification of T. vaginalis compared to Trypan Blue dye exclusion. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5437727 Mon, 31 Oct 2011 04:00:00 +0100 5437727 http://www.medworm.com/index.php?rid=5376554&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05184.x Conclusions:  Overall, similar populations of environmental E.coli and E.coli O157:H7 were recovered from plants despite differences in inoculum dose and agronomic conditions. Strain source had a significant impact on the quantitative level and duration of survival on leaves and in soil. Water availability appeared to be the determinant factor in survival of E.coli and E.coli O157:H7; however E.coli showed greater environmental fitness.Significance and Impact of the Study:  Persistence of surrogate, indicator E. coli and E.coli O157:H7, irrespective of variable growing conditions in spinach is predominantly limited by water availability, strain source and localization within the plant. These findings are anticipated to ultimately be adopted into routine and investigative pathogen testi... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376554 Mon, 31 Oct 2011 04:00:00 +0100 5376554 http://www.medworm.com/index.php?rid=5376553&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05185.x AbstractTrichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract and transmitted primarily by sexual intercourse. We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells. 6 isolates of T. vaginalis were used to assess the utility of the Modified Field′ s stain as a rapid viability test for T. vaginalis and to compare with 0.4% Trypan blue dye exclusion test in three conditions; normal in vitro culture growth using Hollander medium, lysed in distilled water and treated with metronidazole. MF stain showed similar growth profile pattern as trypan blue dye exclusion for identifying... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376553 Mon, 31 Oct 2011 04:00:00 +0100 5376553 http://www.medworm.com/index.php?rid=5376552&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05186.x Conclusions:  The silkworm ‐ C. neoformans infection model is useful for evaluating the therapeutic effects of antifungal drugs.Significance and Impact of the Study:  The silkworm infection model has various advantages for screening antifungal drug candidates. We can also elucidate the cryptococcal pathogenesis and evaluate the in vivo pharmacokinetics and toxicity of each drug. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376552 Mon, 31 Oct 2011 04:00:00 +0100 5376552 http://www.medworm.com/index.php?rid=5356030&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05183.x Conclusions:  Repairing the failing OWTS improved groundwater microbial water quality, though persistence of bacteria in surface water suggests that the OWTS was not the singular fecal contributor to adjacent coastal waters. A suite of tracers is needed to fully assess OWTS performance in treating microbial contaminants and related impacts on receiving waters. Molecular methods like qRT‐PCR have potential but require optimization.Significance and Impact of Study:  This is the first before and after study of a failing OWTS and provides guidance on selection of microbial tracers and methods. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5356030 Fri, 28 Oct 2011 07:23:32 +0100 5356030 http://www.medworm.com/index.php?rid=5344031&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05182.x Conclusions:  Both strains, renamed as Bacillus amyloliquefaciens ARP23 and MEP218, were able to produce antifungal compounds belonging to the cyclic lipopeptide family. Our data suggest that the foliar application of lipopeptide‐producing B. amyloliquefaciens strains could be a promising strategy for the management of sclerotinia stem rot in soybean.Significance and Impact of Study:  Sclerotinia stem rot was ranked as one of the most severe soybean disease in Argentina and worldwide. The results of this study showed the potential of B. amyloliquefaciens strains ARP23 and MEP218 to control plant diseases caused by S. sclerotiorum. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5344031 Mon, 24 Oct 2011 12:13:01 +0100 5344031 http://www.medworm.com/index.php?rid=5437728&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05182.x Conclusions:  Both strains, renamed as B. amyloliquefaciens ARP23 and MEP218, were able to produce antifungal compounds belonging to the cyclic lipopeptide family. Our data suggest that the foliar application of lipopeptide‐producing B. amyloliquefaciens strains could be a promising strategy for the management of sclerotinia stem rot in soybean.Significance and Impact of the Study:  Sclerotinia stem rot was ranked as one of the most severe soybean disease in Argentina and worldwide. The results of this study showed the potential of B. amyloliquefaciens strains ARP23 and MEP218 to control plant diseases caused by S. sclerotiorum. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5437728 Fri, 21 Oct 2011 04:00:00 +0100 5437728 http://www.medworm.com/index.php?rid=5331377&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05180.x Conclusion: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle producing industry.Significance and Impact of Study:  This study is the first report which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331377 Thu, 20 Oct 2011 06:44:16 +0100 5331377 http://www.medworm.com/index.php?rid=5344032&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05181.x SummaryPhytases are a group of enzymes capable of releasing phosphates from phytates, one of the major forms of phosphorus (P) in animal feeds of plant origin. These enzymes have been widely used in animal feed to improve phosphorus nutrition and to reduce phosphorus pollution in animal waste. This review covers the basic nomenclature and crystal structures of phytases, and emphasizes both the protein engineering strategies used for the development of new, effective phytases with improved properties, and the potential biotechnological applications of phytases. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5344032 Thu, 20 Oct 2011 04:00:00 +0100 5344032 http://www.medworm.com/index.php?rid=5331380&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05177.x Conclusions:  The present study observed a tailing phenomenon of transfer of Salmonella during a small‐scale grinding process. It was, therefore, hypothesized that transfer occurred from two environmental matrices inside the grinder and a model was developed. The developed model satisfactorily predicted the observed concentrations of Salmonella during its cross contamination in the grinding of up to 110 pork slices.Significance and Impact of the Study:  The proposed model provides an important tool to examine the effect of cross contamination in quantitative microbial risk assessments and might also be applied to various other food processes where cross contamination is involved. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331380 Tue, 18 Oct 2011 04:00:00 +0100 5331380 http://www.medworm.com/index.php?rid=5331379&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05178.x AbstractThe uptake modes of octadecane by bacteria is an important mechanism for understanding petroleum components biodegradation. An strain that can utilized octadecane well was isolated from crude oil contaminated soil, and named as Pseudomonas sp. DG17 by 16S rDNA analysis. Culture growth result showed that Pseudomonas sp. DG17 grew well in the addition of 200 and 400 mg·L−1 of octadecane, which showed that physical contact between substrate and bacteria was important in the substrate biodegradation. Meanwhile, Pseudomonas sp. DG17 produced rhamnolipids biosurfactant that contains 10 congeners, thus causing the surface tension of the culture medium decline and facilitating the contact between hydrocarbon and bacteria. Scanning‐electron‐microscopy results showed that a disruption... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331379 Tue, 18 Oct 2011 04:00:00 +0100 5331379 http://www.medworm.com/index.php?rid=5331378&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05179.x Conclusion:  The CA‐MRSA clone of se‐carrying‐MDR‐ST‐59‐IV/V‐SC VII was identified predominantly in the present study, and this clone might play a significant role in staphylococcal food poisoning in community settings.Significance and Impact of the Study:  To our knowledge, this is the first study focussing on enterotoxin‐carrying CA‐MRSA/MSSA in pets and their owners, and the results support the future warnings in animal‐human bond caused by community‐associated staphylococci in the commonwealth and need to take cautions worldwide. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331378 Tue, 18 Oct 2011 04:00:00 +0100 5331378 http://www.medworm.com/index.php?rid=5376556&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05175.x Conclusions: Escherichia coli O157:H7 can colonize on spinach leaves at 12 or 25°C in a 100% r.h. environment. Organic matter in the inoculum carrier may provide protection and nutrients which enhance survival and colonization.Significance and Impact of the Study:  Colonization of E. coli O157:H7 on spinach leaves as affected by organic matter in the inoculum, temperature and r.h. was determined. These observations will be useful when developing strategies to prevent growth of E. coli O157:H7 on pre‐ and postharvest spinach. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376556 Tue, 11 Oct 2011 04:00:00 +0100 5376556 http://www.medworm.com/index.php?rid=5376555&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05174.x Conclusions:  Serrulatane compounds showed significant activity against medically important bacteria, with 1 exhibiting stronger antibacterial activity. However, they also displayed toxicity to mammalian cells.Significance and Impact of the Study:  Serrulatanes are of interest as novel antibacterial compounds for use in biomedical applications; this study reports data obtained with a range of bacterial strains and mammalian cells, essential for assessing the capabilities and limitations of potential applicability of these compounds. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376555 Tue, 11 Oct 2011 04:00:00 +0100 5376555 http://www.medworm.com/index.php?rid=5311536&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05173.x Conclusions:  The marine sponge H. simulans harbours a diverse collection of endospore‐forming bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture‐dependent and independent methods that do not specifically target sporeformers.Significance and Impact of Study:  Marine sponges are an as yet largely untapped and poorly understood source of endospore‐forming bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterisation of complex microbial populations such as those found in marine sponges. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5311536 Tue, 11 Oct 2011 04:00:00 +0100 5311536 http://www.medworm.com/index.php?rid=5311535&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05175.x Conclusions: E. coli O157:H7 can colonize on spinach leaves at 12 or 25°C in a 100% RH environment. Organic matter in the inoculum carrier may provide protection and nutrients which enhance survival and colonization.Significance and impact of the study:  Colonization of E. coli O157:H7 on a spinach leaves as affected by organic matter in the inoculum, temperature, and RH were determined. These observations will be useful when developing strategies to prevent growth of E. coli O157:H7 on pre‐ and post‐harvest spinach. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5311535 Tue, 11 Oct 2011 04:00:00 +0100 5311535 http://www.medworm.com/index.php?rid=5311534&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05174.x Conclusions:  Serrulatane compounds showed significant activity against medically important bacteria, with 1 exhibiting stronger antibacterial activity. However they also displayed toxicity to mammalian cells.Significance and Impact of the Study:  Serrulatanes are of interest as novel antibacterial compounds for use in biomedical applications; this study reports data obtained with a range of bacterial strains and mammalian cells, essential for assessing the capabilities and limitations of potential applicability of these compounds. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5311534 Tue, 11 Oct 2011 04:00:00 +0100 5311534 http://www.medworm.com/index.php?rid=5302084&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05172.x Conclusion:  The DiversiLab® System, with serogroup databases, was successfully implemented as an adjunct for reference serotyping of Salmonella enterica.Significance and Impact of Study:  The DiversiLab® System platform is a cost‐effective and easy‐to‐use system, which can putatively determine Salmonella enterica serotypes within a few hours. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5302084 Mon, 10 Oct 2011 04:00:00 +0100 5302084 http://www.medworm.com/index.php?rid=5376559&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05168.x Conclusions:  This study provided a comprehensive analysis of toxigenic S. aureus isolates from Korean RTE foods and their toxigenicity types. This emphasizes the potential risk of various types of toxigenic Staph. aureus in refrigerated RTE food products, which should be better managed to establish safer food chains in global food markets.Significance and Impact of the Study:  This result may contribute to an extended database on Staph. aureus food contamination and mitigate the lack of available information on microbiological hazards in Southeast Asian Nations. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376559 Tue, 04 Oct 2011 04:00:00 +0100 5376559 http://www.medworm.com/index.php?rid=5376558&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05163.x Conclusions:  Usage of BCN E‐760 only selected low‐level BCNrCamp. jejuni mutants in vivo, and the low‐level BCN resistance was not stable in vitro and in vivo.Significance and Impact of the Study:  The study provides helpful information for risk assessment of the future practical application of the anti‐Campylobacter BCNs in animals. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376558 Tue, 04 Oct 2011 04:00:00 +0100 5376558 http://www.medworm.com/index.php?rid=5376557&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05167.x Conclusions:  The purification yield of the developed extracellular production system was 5 mg recombinant mature Esp per 20‐ml culture, which was much higher than that of an intracellular production system in Escherichia coli (3 mg recombinant Esp per 1‐l culture).Significance and Impact of the Study:  Our findings will be a powerful tool for the production and purification of recombinant Esp and also applicable to a large variety of recombinant proteins used for basic researches and biotechnological applications. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376557 Tue, 04 Oct 2011 04:00:00 +0100 5376557 http://www.medworm.com/index.php?rid=5356031&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05162.x Conclusions:  Isolated polysaccharide was found to be of high molecular weight and thermally stable. The purified EPS fraction was composed of hexose, pentose and deoxyhexose sugar residues, which is a rare combination for bacterial polysaccharides. Emulsifying property was either better or comparable to that of other commercially available natural gums and polysaccharides.Significance and Impact of the Study:  This is probably one of the few reports about characterizing an emulsifying EPS produced by a freshwater sediment‐attached bacterium. The results of this study contribute to understand the influence of chemical composition and material properties of a new microbial polysaccharide on its application in industrial biotechnology. Furthermore, this work reconfirms freshwater dynam... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5356031 Tue, 04 Oct 2011 04:00:00 +0100 5356031 http://www.medworm.com/index.php?rid=5282095&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05162.x Conclusions:  Isolated polysaccharide was found to be of high molecular weight and thermally stable. The purified EPS fraction was composed of hexose, pentose, and deoxyhexose sugar residues, which is a rare combination for bacterial polysaccharides. Emulsifying property was either better or comparable to that of other commercially available natural gums and polysaccharides. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282095 Tue, 04 Oct 2011 04:00:00 +0100 5282095 http://www.medworm.com/index.php?rid=5282094&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05163.x Conclusions:  Usage of BCN E‐760 only selected low‐level BCNrC. jejuni mutants in vivo and the low‐level BCN resistance was not stable in vitro and in vivo. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282094 Tue, 04 Oct 2011 04:00:00 +0100 5282094 http://www.medworm.com/index.php?rid=5282093&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05164.x Conclusions: S. maltophilia AAP56 shows different mechanisms for dye‐decolorization. The gene encoding the laccase has been identified, and it has been shown that it is involved in the in vitro decolorization. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282093 Tue, 04 Oct 2011 04:00:00 +0100 5282093 http://www.medworm.com/index.php?rid=5282092&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05166.x Conclusions:  Biochemical characterization was sensitive for interspecific typing, while PCR based approaches permitted a more accurate discrimination for intraspecific typing resulting in pathogenic and nonpathogenic clusters and further more delicate clades for Edwardsiella. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282092 Tue, 04 Oct 2011 04:00:00 +0100 5282092 http://www.medworm.com/index.php?rid=5282091&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05167.x Conclusions:  The purification yield of the developed extracellular production system was 5 mg recombinant mature Esp per 20 ml culture, which was much higher than that of an intracellular production system in E. coli (3 mg recombinant Esp per 1 l culture). (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282091 Tue, 04 Oct 2011 04:00:00 +0100 5282091 http://www.medworm.com/index.php?rid=5282090&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05165.x Conclusions:  The designed PCR primers identified the Erwinia species unambiguously and can be applied to qualitative and quantitative tests. MALDI‐TOF MS data were in agreement with the PCR assays. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282090 Tue, 04 Oct 2011 04:00:00 +0100 5282090 http://www.medworm.com/index.php?rid=5282089&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05168.x Conclusions:  This study provided a comprehensive analysis of toxigenic S. arueus isolates from Korean RTE foods their toxigenicity types. This emphasizes the potential risk of various types of toxigenic S. aureus in refrigerated RTE food products, which should be better managed to establish safer food chains in global food markets. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282089 Tue, 04 Oct 2011 04:00:00 +0100 5282089 http://www.medworm.com/index.php?rid=5282088&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05169.x Conclusion:  The developed mPCR assay was found to be simple, rapid, reliable and specific in species identification of 10 bacteria at a time. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282088 Tue, 04 Oct 2011 04:00:00 +0100 5282088 http://www.medworm.com/index.php?rid=5311533&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05176.x Conclusions:  Chimeric VLPs containing the insoluble preS1His6HBcAg and highly soluble tHBcAg were produced by using a novel incorporation method. The preS1 ligand was exposed on the surface of the VLPs and was shown to deliver fluorescein molecules into liver cells.Significance and Impact of Study:  The newly established incorporation method can be used in the development of chimeric VLPs which could serve as potential nano‐vehicles to target various cells specifically by substituting the preS1 ligand with different cell specific ligands. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5311533 Sat, 01 Oct 2011 04:00:00 +0100 5311533 http://www.medworm.com/index.php?rid=5302083&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05171.x Conclusions:  A CBP system using Kluyveromyces marxianus is suitable for efficient ethanol production from Jerusalem artichoke tubers under VHG conditions.Significance and Impact:  Jerusalem artichoke tubers are an alternative to grain‐based feedstocks for ethanol production. The high ethanol concentration achieved using Kluyveromyces marxianus with the CBP system not only saves energy consumption for ethanol distillation, but also significantly reduces the amount of waste distillage discharged from the distillation system. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5302083 Sat, 01 Oct 2011 04:00:00 +0100 5302083 http://www.medworm.com/index.php?rid=5282087&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05170.x Conclusion:  Chrysin is a potent inhibitor of α‐haemolysin expression by S. aureus, and it conferred a significant degree of protection against S. aureus pneumonia. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282087 Sat, 01 Oct 2011 04:00:00 +0100 5282087 http://www.medworm.com/index.php?rid=5257706&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05161.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5257706 Tue, 27 Sep 2011 09:17:44 +0100 5257706 http://www.medworm.com/index.php?rid=5257708&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05159.x Conclusion:  Phe was the major stimulant for PLA formation; however Cit could increase both PLA and OH‐PLA synthesis by Lact. plantarum CRL 778 probably due to an increase of oxidized NAD+. This effect, as well as the GDH activity, were enhanced by α‐KG and down regulated by Glu.Significance and impact of study:  This is the first study where the role of Glu and GDH activity in the PLA and OH‐PLA synthesis was evidenced in sourdough LAB using a CDM. These results contribute to the knowledge on the antifungal compounds production by sourdough LAB with potential applications on the baked‐good. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5257708 Mon, 26 Sep 2011 04:00:00 +0100 5257708 http://www.medworm.com/index.php?rid=5257707&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05160.x Conclusions:  No significant differences were observed between the parental strain and the TCS‐defective mutants. p‐coumaric acid elicited a response against membrane and cytoplasmic damages.Significance and Impact of the Study:  The inhibitory effect of phenolic compounds on Lact. casei BL23 has been determined. For the first time, cytoplasmic proteins presumably involved in the response of Lact. casei BL23 against p‐coumaric acid have been identified. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5257707 Mon, 26 Sep 2011 04:00:00 +0100 5257707 http://www.medworm.com/index.php?rid=5246930&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05158.x Conclusions:  These studies have demonstrated the potential biocidal use of the novel polyacrylonitrile catalyst when combined with hydrogen peroxide. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246930 Fri, 23 Sep 2011 15:12:20 +0100 5246930 http://www.medworm.com/index.php?rid=5234278&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05154.x Conclusions:  The greater effect of dietary alteration on bacterial community in the rumen compared to the methanogen community may reflect the impact of substrate availability on the rumen bacterial community. This resulted in altered rumen volatile fatty acid profiles and had a downstream effect on methanogen abundance, but not diversity. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234278 Tue, 20 Sep 2011 05:10:45 +0100 5234278 http://www.medworm.com/index.php?rid=5302085&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05157.x AbstractAlthough most vitamins are present in a variety of foods, human vitamin deficiencies still occur in many countries, mainly because of malnutrition not only as a result of insufficient food intake but also because of unbalanced diets. Even though most lactic acid bacteria (LAB) are auxotrophic for several vitamins, it is now known that certain strains have the capability to synthesize water‐soluble vitamins such as those included in the B‐group (folates, riboflavin and vitamin B12 amongst others). This review article will show the current knowledge of vitamin biosynthesis by LAB and show how the proper selection of starter cultures and probiotic strains could be useful in preventing clinical and subclinical vitamin deficiencies. Here, several examples will be presented where vit... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5302085 Tue, 20 Sep 2011 04:00:00 +0100 5302085 http://www.medworm.com/index.php?rid=5294041&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05158.x Conclusions:  These studies have demonstrated the potential biocidal use of the novel polyacrylonitrile catalyst when combined with hydrogen peroxide.Significance and Impact of the Study:  This is the first publication to demonstrate the enhanced activity gained using the novel heterogeneous catalyst to potentiate the activity of hydrogen peroxide as a biocide. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5294041 Tue, 20 Sep 2011 04:00:00 +0100 5294041 http://www.medworm.com/index.php?rid=5246933&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05155.x Conclusions:  The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down‐regulated TNF‐α and up‐regulated IL‐10 in serum and attenuated the extent of damage to the lung and liver. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246933 Tue, 20 Sep 2011 04:00:00 +0100 5246933 http://www.medworm.com/index.php?rid=5246932&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05156.x Conclusion:  These evidences indicate that the surface‐displayed VP28 on B. subtilis spore could induce protection of crayfish against WSSV via oral administration (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246932 Tue, 20 Sep 2011 04:00:00 +0100 5246932 http://www.medworm.com/index.php?rid=5246931&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05157.x SummaryAlthough most vitamins are present in a variety of foods, human vitamin deficiencies still occur in many countries, mainly due to malnutrition not only as a result of insufficient food intake but also because of unbalanced diets. Even though most Lactic Acid Bacteria (LAB) are auxotrophic for several vitamins, it is now known that certain strains have the capability to synthesize water soluble vitamins such as those included in the B‐group (folates, riboflavin and vitamin B12 among others). This review article will show the current knowledge of vitamin biosynthesis by LAB and show how the proper selection of starter cultures and probiotic strains could be useful in preventing clinical and sub‐clinical vitamin deficiencies. Here, several examples will be presented where vitamin p... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246931 Tue, 20 Sep 2011 04:00:00 +0100 5246931 http://www.medworm.com/index.php?rid=5234279&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05153.x Conclusions:  These results indicate that lacticin 3147 may be an effective therapy against S. mutans and was shown to substantially attenuate its ability to form a biofilm. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234279 Fri, 16 Sep 2011 04:00:00 +0100 5234279 http://www.medworm.com/index.php?rid=5294039&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05152.x Conclusions:  Isolates within the serovar show high levels of genetic relatedness regardless of the source. The data indicates S. Virchow is an invasive and inflammatory serovar, consistent with its association with invasive salmonellosis in humans.Significance and Impact of the Study:  The poultry infection experiment included in this study shows S. Virchow can colonize the gastrointestinal tract rapidly and to high levels with the chickens showing no clinical signs of infection. The asymptomatic colonization of chickens indicates an increased ability of S. Virchow to enter the food chain undetected and cause human salmonellosis which because of the invasive and inflammatory nature of S. Virchow seen during the Caco2 invasion assay and previous studies showing its invasive nature ... Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5294039 Thu, 15 Sep 2011 04:00:00 +0100 5294039 http://www.medworm.com/index.php?rid=5234281&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05151.x Conclusions:  Organic matter degradation was mainly governed by a common microbial assemblage consisting of many lineages belonging to the Class Bacilli. The timing of community development differed depending on aeration intensity. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234281 Thu, 15 Sep 2011 04:00:00 +0100 5234281 http://www.medworm.com/index.php?rid=5234280&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05152.x Conclusions:  Isolates within the serovar show high levels of genetic relatedness regardless of the source. The data indicates S. Virchow is an invasive and inflammatory serovar, consistent with its association with invasive salmonellosis in humans. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234280 Thu, 15 Sep 2011 04:00:00 +0100 5234280 http://www.medworm.com/index.php?rid=5218236&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05150.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5218236 Wed, 14 Sep 2011 11:25:02 +0100 5218236 http://www.medworm.com/index.php?rid=5282098&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05148.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282098 Tue, 13 Sep 2011 04:00:00 +0100 5282098 http://www.medworm.com/index.php?rid=5282097&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05149.x Conclusions:  The results suggest that in Fl. columnare both rhizoid colony morphology and high chondroitinase activity are needed for virulence and that temperature may promote the adhesion of the virulent variants to surfaces at fish farms.Significance and Impact of the Study:  New information is produced on the virulence mechanisms of Fl. columnare and the reasons behind the survival of the bacterium at fish farms. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282097 Tue, 13 Sep 2011 04:00:00 +0100 5282097 http://www.medworm.com/index.php?rid=5282096&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05150.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282096 Tue, 13 Sep 2011 04:00:00 +0100 5282096 http://www.medworm.com/index.php?rid=5218238&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05148.x (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5218238 Tue, 13 Sep 2011 04:00:00 +0100 5218238 http://www.medworm.com/index.php?rid=5218237&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05149.x Conclusions:  The results suggest that in F. columnare both rhizoid colony morphology and high chondroitinase activity are needed for virulence and that temperature may promote the adhesion of the virulent variants on surfaces at fish farms. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5218237 Tue, 13 Sep 2011 04:00:00 +0100 5218237 http://www.medworm.com/index.php?rid=5269766&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05142.x Conclusions:  The results indicated that the inoculation of strain D14 could contribute to the increase in the As tolerance of P. deltoides, promotion of the growth, increase in the uptake efficiency and enhancement of As translocation.Significance and Impact of the Study:  The use of P. deltoides in combination with the inoculation of strain D14 provides a potential application for efficient soil arsenic bioremediation. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5269766 Mon, 05 Sep 2011 04:00:00 +0100 5269766 http://www.medworm.com/index.php?rid=5246934&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05145.x Conclusions:  An exoinulinase gene of P. janthinellum strain B01 was cloned, sequenced and over‐expressed successfully in P. pastoris.Significance and Impact of the Study:  Only a few genes have been cloned from P. janthinellum because its molecular biology is poorly understood. In this study, we cloned and over‐expressed inuA1 gene of P. janthinellum in P. pastoris. This recombinant exoinulinase can be used to hydrolyse inulin to produce fructose and facilitate the biofuel production from inulin resources. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246934 Mon, 05 Sep 2011 04:00:00 +0100 5246934 http://www.medworm.com/index.php?rid=5204871&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05143.x Conclusions:  Quorum sensing mediated by the auto‐inducer 2 molecule plays a significant role in both B. anthracis growth and toxin production. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204871 Mon, 05 Sep 2011 04:00:00 +0100 5204871 http://www.medworm.com/index.php?rid=5204870&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05142.x Conclusions:  The results indicated that the inoculation of strain D14 could contribute to the increase of the As tolerance of P. deltoides, promotion of the growth, increase of the uptake efficiency and enhancement of As translocation. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204870 Mon, 05 Sep 2011 04:00:00 +0100 5204870 http://www.medworm.com/index.php?rid=5204869&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05145.x Conclusions:  An exoinulinase gene of P. janthinellum strain B01 was cloned, sequenced and over‐expressed successfully in P. pastoris. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204869 Mon, 05 Sep 2011 04:00:00 +0100 5204869 http://www.medworm.com/index.php?rid=5204868&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05144.x Conclusion:  Only those hygienic risks relating to mesophilic vegetative bacteria can be totally eliminated from pig slurry treated at 60°C (60 min) or 70°C (less than 10 min). (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204868 Mon, 05 Sep 2011 04:00:00 +0100 5204868 http://www.medworm.com/index.php?rid=5204867&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05146.x Conclusions: The in vitro cell assay might be initially used to screen large quantities of bacteria to select attenuated mutants of catfish pathogens. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204867 Mon, 05 Sep 2011 04:00:00 +0100 5204867 http://www.medworm.com/index.php?rid=5246935&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05141.x Conclusions:  The influence of lipophilicity on the antibacterial activity of hydroxybenzoic acids is stronger than that of hydroxycinnamic acids. Metabolism of phenolic acids by LAB detoxifies phenolic acids.Significance and Impact of the Study:  Results allow the targeted selection of plant extracts for food preservation, and selection of starter cultures for fermented products. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246935 Fri, 02 Sep 2011 04:00:00 +0100 5246935 http://www.medworm.com/index.php?rid=5204866&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05147.x Conclusions:  The results of this study revealed the different immunomodulatory properties of strains of the species L. paracasei. Strain IMPC 4.1 showed an interesting anti‐inflammatory ability. Probiotic strains IMPC 2.1 and LMG P‐17806 were characterized by a similar and intermediate ability to induce cytokine secretion in contrast to the very low ability of strain LMG 23554. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204866 Thu, 01 Sep 2011 04:00:00 +0100 5204866 http://www.medworm.com/index.php?rid=5191680&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05141.x Conclusions:  The influence of lipophilicity on the antibacterial activity of hydroxybenzoic acids is stronger than that of hydroxycinnamic acids. Metabolism of phenolic acids by LAB detoxifies phenolic acids. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5191680 Wed, 31 Aug 2011 23:00:00 +0100 5191680 http://www.medworm.com/index.php?rid=5174133&cid=s_32055_77_f&fid=32055&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2672.2011.05140.x Conclusions:  It was confirmed that soil bioaugmentation with Pseudomonas sp. JS150 significantly enhanced soil activity towards phenol degradation. Cyclopropane and branched fatty acids were sensitive probes for degree of phenol utilization. (Source: Journal of Applied Microbiology) Journal of Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5174133 Tue, 30 Aug 2011 03:25:18 +0100 5174133