Journal of Biophotonics via MedWorm.com MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Biophotonics' source. Mon, 06 Feb 2012 20:24:50 +0100 In vivo measurements of diffuse reflectance and time‐resolved autofluorescence emission spectra of basal cell carcinomas http://www.medworm.com/index.php?rid=5664522&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100126 We present a clinical investigation of diffuse reflectance and time‐resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady‐state diffuse reflectance/fluorescence spectrometer and a fibre‐optic‐coupled multispectral time‐resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave‐one‐out methodology. This approach was able to correctly diagnose... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5664522 Mon, 06 Feb 2012 05:00:00 +0100 5664522 Quantitative discrimination of NPC cell lines using optical coherence tomography http://www.medworm.com/index.php?rid=5664521&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100121 AbstractWe tried to explore the intrinsic differences in the optical properties of the four representative NPC cell lines on the models of radiobiology and metastasis by OCT. The scattering coefficients and anisotropies were extracted by fitting the average a‐scan attenuation curves based on the multiple scatter effect. The values of scattering coefficients and anisotropy factors were 5.21 ± 0.11, 5.30 ± 0.09, 5.92 ± 0.21, 6.97 ± 0.22, and 0.892 ± 0.009, 0.886 ± 0.006, 0.884 ± 0.009, 0.86 ± 0.01 for CNE1, CNE2, 5‐8F and 6‐10B pellets (p < 0.05, P = 0.07 for CNE1 and CNE2), respectively. The results showed that the radiobiology and metastasis cell's model could be distinguished obviously; which implied that the corresponding types of NPC tissue might be potentially differen... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5664521 Mon, 06 Feb 2012 05:00:00 +0100 5664521 Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry http://www.medworm.com/index.php?rid=5664520&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100113 AbstractWe use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess‐light conditions on the physiological state of the green alga Chlorella pyrenoidosa. We demonstrate that zeaxanthin‐dependent non‐photochemical quenching leads to a significant increase in the rate constant of singlet‐singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light‐harvesting complexes of photosystem II. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5664520 Mon, 06 Feb 2012 05:00:00 +0100 5664520 Influence of skin aging effects on the skin surface profile and the correlated distribution of topically applied sunscreens http://www.medworm.com/index.php?rid=5664519&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100104 AbstractThe surface profile of human skin influences characteristically the distribution pattern of topically applied formulations and consequently the efficacy of sunscreens. The volumes of furrows and the spectroscopically determined factors of inhomogeneity are investigated for three sunscreens. A clear correlation between both measurands exists. The average values for younger (<32 years) and older (≥51 years) volunteers do not show statistically significant differences. Systematic variations found for the individual values are due to a reduced elasticity of the skin with age improving the homogeneity of the sunscreen distribution. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5664519 Wed, 01 Feb 2012 05:00:00 +0100 5664519 A compartment‐specific transcriptome analysis reveals survival‐relevant marker genes in the stroma fraction of squamous non‐small cell lung cancer http://www.medworm.com/index.php?rid=5635779&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100115 This study presents a compartment‐specific transcriptome analysis of lung squamous cell carcinoma (SCC) samples microdissected into tumor parenchyma and stroma fractions. Typical tumor and stroma genes were identified based on the expression ratios between the two compartments. Our results indicate that in SCC many markers related to longer survival are predominantly expressed in the stroma, particularly genes of the MHC‐II complex. Stromal upregulation of MHC‐II genes seems crucial for a clinically relevant antitumor immune response in SCC. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5635779 Sat, 28 Jan 2012 01:35:20 +0100 5635779 Tracking of nanoscale structural variations on a single amyloid fibril with tip‐enhanced Raman scattering http://www.medworm.com/index.php?rid=5625077&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100142 AbstractAmyloid fibrils are known to be responsible for diseases such as Alzheimer's disease. A detailed insight into the structure of amyloid fibrils is fundamental since it is not yet understood what triggers the misfolding of proteins to the fiber like structures. The molecular structure of fibril surfaces on a single amino acid level has not been revealed so far but would present a valuable contribution to this question. Here we demonstrate the direct molecular distinction of selected amino acids on insulin fibril surfaces with a lateral resolution better than 2 nm by applying tip‐enhanced Raman spectroscopy (TERS). This approach provides simultaneously a way to directly reveal conformational changes in the secondary structure, namely α‐helix, β‐sheet, on the fibril surface wit... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625077 Wed, 25 Jan 2012 02:27:05 +0100 5625077 The utility of far‐infrared illumination in oxygenation dynamics as measured with near‐infrared spectroscopy http://www.medworm.com/index.php?rid=5625082&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100108 AbstractNear‐infrared spectroscopy (NIRS) is a noninvasive method for measuring the oxygenation in muscle and other tissues in vivo. For quantitative NIRS measurement of oxygenation dynamics, the vessel‐occlusion test was usually applied as physiological intervention. There are several drawbacks of the vessel‐occlusion method that include skin contact, uncomfortable and microcirculation block of patients. Thus, we propose the far‐infrared (FIR) illumination as a new physiological intervention method in this paper. Our preliminary result shows a linear correlation of oxygenation dynamic signals between FIR illumination and arterial‐occlusion test (AOT) that implies the FIR illumination could be applied for hemodynamic response measurement in clinical diagnosis. (© 2011 WILEY‐VC... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625082 Mon, 23 Jan 2012 05:00:00 +0100 5625082 Technologies supporting analytical cytology: clinical, research and drug discovery applications http://www.medworm.com/index.php?rid=5625081&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100093 AbstractThe tools and techniques developed for analytical cytology have become invaluable in expanding the development of cancer screening programs and biomarker discovery for personalized medicine. Detecting cellular, molecular, and functional changes of diseased tissue as defined by quantitative analytical methodologies has enhanced the field of medical diagnostics and prognostics. The focus of this review is to outline applications and recent technical advances in flow cytometry, laser scanning cytometry, image cytometry, and quantitative image analysis, as they pertain to clinical, research, and drug discovery applications. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625081 Mon, 23 Jan 2012 05:00:00 +0100 5625081 Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy http://www.medworm.com/index.php?rid=5625080&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100080 AbstractBased on compelling in vivo and in vitro studies on human skin, carotenoids are thought to be of great interest as powerful antioxidants acting to prevent free‐radical‐induced damages, including premature skin ageing and the development of skin diseases such as cancer. Among the available techniques that are suitable for noninvasive determination of carotenoids in human skin, are resonance Raman spectroscopy (RRS) and reflection spectroscopy (RS). For RS, a LED‐based miniaturized spectroscopic system (MSS) was developed for noninvasive measurement of carotenoids in human skin. The optimization and subsequent calibration of the MSS was performed with the use of RRS. A strong correlation between the carotenoid concentration determined by the RS and for the RRS system was achiev... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625080 Mon, 23 Jan 2012 05:00:00 +0100 5625080 In vivo imaging of hepatic excretory function in the rat by fluorescence microscopy http://www.medworm.com/index.php?rid=5625079&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100118 AbstractApplying intravital fluorescence microscopy, we assessed sinusoidal delivery and biliary clearance of two different polymethine dyes. DY635, a benzopyrylium‐based hemocyanine dye with shorter excitation wavelength than indocyanine green (ICG), was validated for assessment of hepatic excretory function. Decrease of DY635 and ICG reflecting transcellular transport was 83 ± 4% (DY635) and 14 ± 2% (ICG; p < 0.05) over 35 minutes, respectively. In cholestasis, hepatobiliary excretion of DY635 was markedly impaired (control 3176 ± 148 pmol vs. cholestatic 1929 ± 179 pmol; p < 0.05). DY635 even enabled an analysis at high resolution suggesting 1.) hepatocyte uncoupling and 2.) failure of primarily the canalicular pole, allowing in vivo insights into molecular mechanisms of thi... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625079 Mon, 23 Jan 2012 05:00:00 +0100 5625079 Fibrillogenesis of human β2‐microglobulin in three‐dimensional silicon microstructures http://www.medworm.com/index.php?rid=5625078&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100132 AbstractThe authors describe the interaction of biological nanostructures formed by β2‐microglobulin amyloid fibrils with three‐dimensional silicon microstructures consisting in periodic arrays of vertical silicon walls (≈3 μm‐thick) separated by 50 μm‐deep air gaps (≈5 μm‐wide). These structures are of great interest from a biological point of view since they well mimic the interstitial environment typical of amyloid deposition in vivo. Moreover, they behave as hybrid photonic crystals, potentially applicable as optical transducers for label‐free detection of the kinetics of amyloid fibrils formation. Fluorescence and atomic force microscopy (AFM) show that a uniform distribution of amyloid fibrils is achieved when fibrillogenesis occurs directly on silicon. The high r... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5625078 Mon, 23 Jan 2012 05:00:00 +0100 5625078 Contents: J. Biophotonics 2/2012 http://www.medworm.com/index.php?rid=5617355&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201200501 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5617355 Sun, 22 Jan 2012 01:20:48 +0100 5617355 Issue Information: J. Biophotonics 2/2012 http://www.medworm.com/index.php?rid=5617354&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201290001 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5617354 Sun, 22 Jan 2012 01:20:46 +0100 5617354 Cover Picture: J. Biophotonics 2/2012 http://www.medworm.com/index.php?rid=5617353&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201290000 AbstractScars typically evolve through a series of successive stages with characteristic tissue morphologies. These panels show second harmonic generation images of, from left to right, normal skin tissue, hypertrophic scar and mature scar that are precisely diagnosed by a computer. Autofluorescence is shown green. Image height: 1.5 mm. (Picture: T. Kelf et al., pp 159–167 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5617353 Sun, 22 Jan 2012 01:20:45 +0100 5617353 Fourier transform infrared microspectroscopy identifies protein propionylation in histone deacetylase inhibitor treated glioma cells http://www.medworm.com/index.php?rid=5617352&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100061 AbstractHistone deacetylase inhibitors (HDIs) have attracted considerable attention as potential drug molecules in tumour biology. In order to optimise chemotherapy, it is important to understand the mechanisms of regulation of histone deacetylase (HDAC) enzymes and modifications brought by various HDIs. In the present study, we have employed Fourier transform infrared microspectroscopy (FT‐IRMS) to evaluate modifications in cellular macromolecules subsequent to treatment with various HDIs. In addition to CH3 (methyl) stretching bands at 2872 and 2960 cm–1, which arises due to acetylation, we also found major changes in bands at 2851 and 2922 cm–1, which originates from stretching vibrations of CH2 (methylene) groups, in valproic acid treated cells. We further demonstrate that the ch... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5617352 Sun, 22 Jan 2012 01:20:28 +0100 5617352 Hyperspectral imaging of mucosal surfaces in patients http://www.medworm.com/index.php?rid=5577013&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100081 AbstractThe aim of this study was to proof applicability of hyperspectral imaging for the analysis and classification of human mucosal surfaces in vivo. The larynx as a prototypical anatomically well‐defined surgical test area was analyzed by microlaryngoscopy with a polychromatic lightsource and a synchronous triggered monochromatic CCD‐camera. Image stacks (5 benign, 7 malignant tumors) were analyzed by established software (principal component analysis PCA, hyperspectral classification, spectral profiles). Hyperspectral image datacubes were analyzed and classified by conventional software. In PCA, images at 590–680 nm loaded most onto the first PC which typically contained 95% of the total information. Hyperspectral classification clustered the data highlighting altered mucosa. Th... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5577013 Mon, 09 Jan 2012 05:00:00 +0100 5577013 CRAFT: Multimodality confocal skin imaging for early cancer diagnosis http://www.medworm.com/index.php?rid=5577012&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100124 AbstractAlthough histological analysis serves as a gold standard to cancer diagnosis, its application on skin cancer detection is largely prohibited due to its invasive nature. To obtain both the structural and pathological information in situ, a Confocal Reflectance/Auto‐Fluorescence Tomography (CRAFT) system was established to examine the skin sites in vivo with both reflectance and autofluorescence modes simultaneously. Nude mice skin with cancerous sites and normal skin sites were imaged and compared with the system. The cellular density and reflective intensity in cancerous sites reflects the structural change of the tissue. With the decay coefficient analysis, the corresponding NAD(P)H decay index for cancerous sites is 1.65‐fold that of normal sites, leading to a 97.8% of sensit... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5577012 Mon, 09 Jan 2012 05:00:00 +0100 5577012 In‐vivo Tumor detection using diffusion reflection measurements of targeted gold nanorods – a quantitative study http://www.medworm.com/index.php?rid=5593680&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100120 In this study, Monte Carlo (MC) simulations, tissue‐like phantom experiments and in‐vivo measurements of the reflected light intensity from tumor bearing mice are presented. Following intravenous injection of antibody conjugated poly (ethylene glycol)‐coated (PEGylated) gold nanorods (GNR) to tumor‐bearing mice, accumulation of GNR in the tumor was clearly detected by the DR profile of the tumor. The ability of DR measurements to quantitate in‐vivo the concentration of the GNR in the tumor was demonstrated and validated with Flame Atomic Absorption spectroscopy results. With GNR as absorbing contrast agents, DR has important potential applications in the image guided therapy of superficial tumors such as head and neck cancer, breast cancer and melanoma. (© 2011 WILEY‐VCH Verla... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5593680 Sun, 01 Jan 2012 05:00:00 +0100 5593680 Molecular classification of breast carcinomas with particular emphasis on “basal‐like” carcinoma: A critical review http://www.medworm.com/index.php?rid=5577011&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100097 AbstractDuring the last 11 years, 5 molecular subtypes of breast carcinoma (luminal A, luminal B, Her2‐positive, basal‐like, and normal breast‐like) have been characterized and intensively studied. As genomic research evolves, further subtypes of breast cancers into new “molecular entities” are expected to occur. For example, a new and rare breast cancer subtype, known as claudin‐low, has been recently found in human carcinomas and in breast cancer cell lines. There is no doubt that global gene expression analyses using high‐throughput biotechnologies have drastically improved our understanding of breast cancer as a heterogeneous disease. The main question is, however, whether new molecular techniques such as gene expression profiling (or signature) should be regarded as the ... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5577011 Sun, 01 Jan 2012 05:00:00 +0100 5577011 Digital imaging in pathology – current applications and challenges http://www.medworm.com/index.php?rid=5550027&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100103 This article provides an overview of current digital pathology applications and research with emphasis on whole slide imaging (WSI). Static or interactive digital pathology work stations already can be used for many purposes, e.g. telepathology expert consultations, frozen section diagnosis in remote areas, cytology screening, quality assurance, diagnostic validations for clinical trials, quantitation of hormone receptor or HER2 studies in breast cancer, or three‐dimensional visualization of anatomical structures, among others. Changes of workflow in histology laboratories are beginning to enable digital image acquisition and WSI in a routine setting. WSI plays an increasing role in pathology education, glass slide boxes in medical schools are being replaced by digital slide collections;... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5550027 Thu, 29 Dec 2011 18:48:03 +0100 5550027 Investigation of the permeability and optical clearing ability of different analytes in human normal and cancerous breast tissues by spectral domain OCT http://www.medworm.com/index.php?rid=5550028&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100106 AbstractThe potential of OCT applied for early breast cancer detection attracted significant efforts. The permeability coefficients and the percentages of OCT signal enhancement for normal and cancerous breast tissues have been investigated from the experiments of 20% glucose, 40% glucose, and 20% mannitol in vitro. Obtained results indicate that the permeability coefficient in breast cancer tissue is prominently larger than that in normal breast tissue, while the optical clearing effect is just the reverse to that for each agent. The results suggest that OCT has the ability to distinguish cancer tissue from different aspect. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5550028 Thu, 29 Dec 2011 05:00:00 +0100 5550028 Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging http://www.medworm.com/index.php?rid=5520789&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100098 AbstractA method is presented for acquiring high‐spatial‐resolution spectral maps, in particular for Raman micro‐spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating the results. This method achieves up to 30 times reduction in the sampling time compared to raster‐scanning, the resulting images have excellent correlation with conventional histopathological staining, and are achieved with sufficient spectral signal‐to‐noise ratio to identify individual tissue structures. The benefits of this selective sampling method are not limited to tissue imaging however; it is expected that the method may be applied to other techniques which employ point‐by‐point mapping of large substrates. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinh... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5520789 Tue, 20 Dec 2011 13:19:59 +0100 5520789 Spectroscopic and histological evaluation of wound healing progression following Low Level Laser Therapy (LLLT) http://www.medworm.com/index.php?rid=5512860&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100089 AbstractThe present study focuses on the evaluation of the effect of He‐Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post‐wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non‐illuminated controls. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5512860 Sat, 17 Dec 2011 13:50:52 +0100 5512860 Potentially malignant lesions of the upper aerodigestive tract http://www.medworm.com/index.php?rid=5501450&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100085 AbstractMucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar phenotype but caused by different reasons such as viral infection may be difficult. Furthermore, a magnitude of similar appearing lesions with different clinical background and different biological behavior are discussed in this paper. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5501450 Wed, 14 Dec 2011 15:16:51 +0100 5501450 Journal of Biophotonics – the Biophotonics platform http://www.medworm.com/index.php?rid=5483699&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100513 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5483699 Thu, 08 Dec 2011 14:34:20 +0100 5483699 Contents: J. Biophotonics 1/2012 http://www.medworm.com/index.php?rid=5483698&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100512 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5483698 Thu, 08 Dec 2011 14:34:18 +0100 5483698 Issue Information: J. Biophotonics 1/2012 http://www.medworm.com/index.php?rid=5483697&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190018 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5483697 Thu, 08 Dec 2011 14:34:17 +0100 5483697 Back Cover: J. Biophotonics 1/2012 http://www.medworm.com/index.php?rid=5483696&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190017 AbstractHyperspectral imaging microscopy of rat lung cryoslices can be used to identify individual pulmonary microvascular endothelial cells (PMVECs) in the presence of a high lung autofluorescence of the same peak fluorescence emission wavelength. PMVECs expressing green fluorescent protein are shown in green, lung autofluorescence is shown in red, and nuclei are shown in blue. A plot of the spectral library used for linear unmixing is also shown. (Picture: S. Leavesley et al., pp. 67–84 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5483696 Thu, 08 Dec 2011 14:34:16 +0100 5483696 Cover Picture: J. Biophotonics 1/2012 http://www.medworm.com/index.php?rid=5483695&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190016 AbstractRaman spectroscopy has been used in this study to obtain biochemical fingerprint patterns of collagen fibers in native aortic heart valve tissues. Using this non‐contact screening tool, we were able to monitor the increasing damage of collagen fibers due to enzymatic treatment or cryopreservation. (Picture: M. Votteler et al., pp. 47–56 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5483695 Thu, 08 Dec 2011 14:34:14 +0100 5483695 Label‐free identification and characterization of human pluripotent stem cell‐derived cardiomyocytes using second harmonic generation (SHG) microscopy http://www.medworm.com/index.php?rid=5418251&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100077 We report a second harmonic generation (SHG) signal detectable in PSC‐CMs that is attributable to sarcomeric myosin, dependent on PSC‐CM maturity, and retained while PSC‐CMs are in suspension. Our study demonstrates the feasibility of developing a SHG‐activated flow cytometer for the non‐invasive purification of PSC‐CMs. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5418251 Tue, 15 Nov 2011 05:00:00 +0100 5418251 Laser tissue welding analyzed using fluorescence, Stokes shift spectroscopy, and Huang‐Rhys parameter http://www.medworm.com/index.php?rid=5397253&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100050 AbstractNear infrared (NIR) continuous wave laser radiation at the 1,450 nm wavelength was used to weld porcine aorta and skin samples via the absorption of combitional vibrational modes of native water in the tissues. The fluorescence spectra were measured from the key native molecules of welded and non‐welded tissues at specific excitation and emission wavelengths from collagen, elastin, and tryptophan. The changes in the fluorescence intensities and differences in Stokes shift (Δνss) of key native fluorophores were measured to differentiate the Huang‐Rhys parameter values (S) of the chromophores. The strength of coupling depends on the local electron‐vibration intra‐tissue molecular environment and the amount of polar solvent water surrounding the net charges on collagen, elas... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5397253 Fri, 11 Nov 2011 05:00:00 +0100 5397253 Novel thermal effect at nanoshell heating by pulsed laser irradiation: hoop‐shaped hot zone formation http://www.medworm.com/index.php?rid=5397252&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100074 In this study we have developed an approach for calculation of nanoparticle temperature field accounting for absorbed local intensity at pulse laser radiation of composite spherical nanoparticles (nanoshells). This approach allowed us to analyze spatial inhomogeneities of light field diffracted into a nanoshell and corresponding distribution of the absorption energy and to provide numerical solution of time‐dependent heat conduction equation accounting for corresponding spatially inhomogeneous distribution of heating sources. We were able to predict the appearance of a novel thermal effect – hoop‐shaped hot zone on the nanoshell surface. The observed effect has potential applications in cell biology and medicine for controlled cell optoporation and nanosurgery, as well as cancer cell... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5397252 Fri, 11 Nov 2011 05:00:00 +0100 5397252 Comparison between TEWL and laser scanning microscopy measurements for the in vivo characterization of the human epidermal barrier http://www.medworm.com/index.php?rid=5376119&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100067 AbstractThe analysis of the skin barrier properties is important in various fields of medical treatment and cosmetology. The development and improvement of topically applied substances require an objective analysis of the skin barrier characteristics. Transepidermal water loss (TEWL) measurement is the standard method to characterize epidermal barrier function. The most important disadvantage of this method though, is that it can be affected by different exogenous and endogenous factors, e.g. water content of the applied formulation and room temperature. In the present study, TEWL measurements are compared to laser scanning microscopic (LSM) measurements, concerning the use of these two methods for the non‐invasive in vivo characterization of the epidermal barrier function. The investiga... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376119 Wed, 02 Nov 2011 04:00:00 +0100 5376119 Two‐photon excited fluorescence lifetime measurements through a double‐clad photonic crystal fiber for tissue micro‐endoscopy http://www.medworm.com/index.php?rid=5376118&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100070 AbstractThis paper presents an endoscopic configuration for measurements of tissue autofluorescence using two–photon excitation and time‐correlated single photon counting detection through a double‐clad photonic crystal fiber (DC‐PCF) without pre‐chirping of laser pulses. The instrument performance was evaluated by measurements of fluorescent standard dyes, biological fluorophores (collagen and elastin), and tissue specimens (muscle, cartilage, tendon). Current results demonstrate the ability of this system to accurately retrieve the fluorescence decay profile and lifetime of these samples. This simple setup, which offers larger penetration depth than one‐photon‐based techniques, may be combined with morphology‐yielding techniques such as photoacoustic and ultrasound imagin... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376118 Wed, 02 Nov 2011 04:00:00 +0100 5376118 Second harmonic imaging of plants tissues and cell implosion using two‐photon process in ZnO nanoparticles http://www.medworm.com/index.php?rid=5376116&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100076 AbstractThe optical properties of colloidal ZnO nanoparticle (NP) solutions, with size ranging from several nm to around 200 nm, have been tailored to have high optical nonlinearity for bioimaging with no auto‐fluorescence above 750 nm and minimal auto‐fluorescence below 750 nm. The high second harmonic conversion efficiency enables selective tissue imaging and cell tracking using tunable near‐infrared femtosecond laser source ranging from 750‐980 nm. For laser energies exceeding the two‐photon energy of the bandgap of ZnO (half of 3.34 eV), the SHG signal greatly decreases and the two‐photon emission becomes the dominant signal. The heat generated due to two‐photon absorption within the ZnO NPs enable selective cell or localized tissue destruction using excitation wavelength... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376116 Wed, 02 Nov 2011 04:00:00 +0100 5376116 Bioluminescence imaging of energy depletion in vascular pathology: patent ductus arteriosus and atherosclerosis http://www.medworm.com/index.php?rid=5465514&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100096 AbstractThe artery wall may develop energy depletion due to insufficient nutritional supply. However, until recently it has not been possible to validate this hypothesis because no available technology has allowed assessment of energy metabolism with sufficient spatial resolution. We use high resolution bioluminescence metabolic imaging to study energy metabolism in two mechanistically different vascular pathologies: patent ductus arteriosus and atherosclerosis. Physiological energy depletion in postnatally constricting ductus arteriosus promotes permanent closure. Insufficient ductus energy depletion, common in preterm infants, is associated with persistent patent ductus arteriosus, a condition with significantly increased morbidity and mortality. In contrast, in atherosclerosis, energy d... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5465514 Tue, 01 Nov 2011 04:00:00 +0100 5465514 Bond‐selective imaging of deep tissue through the optical window between 1600 and 1850 nm http://www.medworm.com/index.php?rid=5454373&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100102 We report the employment of an optical window between 1600 nm and 1850 nm for bond‐selective deep tissue imaging through harmonic vibrational excitation and acoustic detection of resultant pressure waves. In this window where a local minimum of water absorption resides, we found a 5 times enhancement of photoacoustic signal by first overtone excitation of the methylene group CH2 at 1730 nm, compared to the second overtone excitation at 1210 nm. The enhancement allows 3D mapping of intramuscular fat with improved contrast and of lipid deposition inside an atherosclerotic artery wall in the presence of blood. Moreover, lipid and protein are differentiated based on the first overtone absorption profiles of CH2 and methyl group CH3 in this window. (© 2011 WILEY‐VCH Verlag GmbH & Co. K... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5454373 Tue, 01 Nov 2011 04:00:00 +0100 5454373 Microvascular and mitochondrial PO2 simultaneously measured by oxygen‐dependent delayed luminescence http://www.medworm.com/index.php?rid=5447889&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100082 AbstractMeasurement of tissue oxygenation is a complex task and various techniques have led to a wide range of tissue PO2 values and contradictory results. Tissue is compartmentalized in microcirculation, interstitium and intracellular space and current techniques are biased towards a certain compartment. Simultaneous oxygen measurements in various compartments might be of great benefit for our understanding of determinants of tissue oxygenation. Here we report simultaneous measurement of microvascular PO2 (μPO2) and mitochondrial PO2 (mitoPO2) in rats. The μPO2 measurements are based on oxygen‐dependent quenching of phosphorescence of the near‐infrared phosphor Oxyphor G2. The mitoPO2 measurements are based on oxygen‐dependent quenching of delayed fluorescence of protoporphyrin IX... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5447889 Tue, 01 Nov 2011 04:00:00 +0100 5447889 Scar tissue classification using nonlinear optical microscopy and discriminant analysis http://www.medworm.com/index.php?rid=5437570&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100075 AbstractThis paper addresses the scar tissue maturation process that occurs stepwise, and calls for reliable classification. The structure of collagen imaged by nonlinear optical microscopy (NLOM) in post‐burn hypertrophic and mature scar, as well as in normal skin, appeared to distinguish these maturation steps. However, it was a discrimination analysis, demonstrated here, that automated and quantified the scar tissue maturation process. The achieved scar classification accuracy was as high as 96%. The combination of NLOM and discrimination analysis is believed to be instrumental in gaining insight into the scar formation, for express diagnosis of scar and surgery planning. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5437570 Tue, 01 Nov 2011 04:00:00 +0100 5437570 The influence of movement on the localization precision of sub‐resolution particles in fluorescence microscopy http://www.medworm.com/index.php?rid=5418250&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100078 AbstractObtaining sub‐resolution particle positions in fluorescence microscopy images is essential for single particle tracking and high‐resolution localization microscopy. While the localization precision of stationary single molecules or particles is well understood, the influence of particle motion during image acquisition has been largely neglected. Here, we address this issue and provide a theoretical description on how particle motion influences the centroid localization precision, both in case of 2‐D and 3‐D diffusion. In addition, a novel method is proposed, based on dual‐channel imaging, for the experimental determination of the localization precision of moving particles. For typical single particle tracking experiments, we show that the localization precision is approxi... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5418250 Tue, 01 Nov 2011 04:00:00 +0100 5418250 Resonance Raman quantification of the redox state of cytochromes b and cin‐vivo and in‐vitro http://www.medworm.com/index.php?rid=5397251&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100087 AbstractWe observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5397251 Tue, 01 Nov 2011 04:00:00 +0100 5397251 Contents: J. Biophotonics 11–12/2011 http://www.medworm.com/index.php?rid=5376122&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100511 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376122 Tue, 01 Nov 2011 04:00:00 +0100 5376122 Issue Information: J. Biophotonics 11–12/2011 http://www.medworm.com/index.php?rid=5376121&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190015 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376121 Tue, 01 Nov 2011 04:00:00 +0100 5376121 Cover Picture: J. Biophotonics 11–12/2011 http://www.medworm.com/index.php?rid=5376120&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190014 AbstractMouse‐shaped phantom with different organs (top left) and the reconstructed result of the internal bioluminescent source using the efficient iterative shrinkage thresholding algorithm (top right). Segmented heterogeneous model from in vivo mouse micro‐CT images (bottom left) and the inversion reconstruction of the implanted light source in the living mouse based on the efficient bioluminescence tomography method (bottom right). (Picture: C. Qin et al., pp. 824–839 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376120 Tue, 01 Nov 2011 04:00:00 +0100 5376120 Minimally invasive non‐thermal laser technology using laser‐induced optical breakdown for skin rejuvenation http://www.medworm.com/index.php?rid=5376115&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100083 We describe a novel, minimally invasive laser technology for skin rejuvenation by creating isolated microscopic lesions within tissue below the epidermis using laser induced optical breakdown. Using an in‐house built prototype device, tightly focused near‐infrared laser pulses are used to create optical breakdown in the dermis while leaving the epidermis intact, resulting in lesions due to cavitation and plasma explosion. This stimulates a healing response and consequently skin remodelling, resulting in skin rejuvenation effects. Analysis of ex‐vivo and in‐vivo treated human skin samples successfully demonstrated the safety and effectiveness of the microscopic lesion creation inside the dermis. Treatments led to mild side effects that can be controlled by small optimizations of the... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5376115 Tue, 01 Nov 2011 04:00:00 +0100 5376115 Two‐dimensional imaging of spontaneous ultra‐weak photon emission from the human skin: role of reactive oxygen species http://www.medworm.com/index.php?rid=5343812&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100073 In this study, two‐dimensional spontaneous ultra‐weak photon emission from the human skin has been measured using a highly sensitive charged coupled device (CCD) camera. It is demonstrated here that two‐dimensional ultra‐weak photon emission from the human skin increases with the topical application of exogenous ROS in the following order: hydrogen peroxide (H2O2) < superoxide anion radical (O2•–) < hydroxyl radical (HO•). We propose here that the two‐dimensional ultra‐weak photon emission can be used as a non‐invasive tool for the spatial and temporal monitoring of oxidative stress in the human skin. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5343812 Thu, 20 Oct 2011 04:00:00 +0100 5343812 Comparison of permissible source region and multispectral data using efficient bioluminescence tomography method http://www.medworm.com/index.php?rid=5311234&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100049 AbstractAs a novel molecular imaging technology, bioluminescence tomography (BLT) has become an important tool for biomedical research in recent years, which can perform a quantitative reconstruction of an internal light source distribution with the scattered and transmitted bioluminescent signals measured on the external surface of a small animal. However, BLT is severely ill‐posed because of complex photon propagation in the biological tissue and limited boundary measured data with noise. Therefore, sufficient a priori knowledge should be fused for the uniqueness and stability of BLT solution. Permissible source region strategy and spectrally resolved measurements are two kinds of a priori knowledge commonly used in BLT reconstruction. This paper compares their performance with simulat... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5311234 Tue, 11 Oct 2011 04:00:00 +0100 5311234 A correlative approach at characterizing nanoparticle mobility and interactions after cellular uptake http://www.medworm.com/index.php?rid=5311233&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100064 We present data supporting endocytotic uptake of the particles and subsequent active transport to the perinuclear region. The presence of particles in lamellar bodies is proposed as a potential exocytosis route. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5311233 Tue, 11 Oct 2011 04:00:00 +0100 5311233 Depth profiling of gold nanoparticles and characterization of point spread functions in reconstructed and human skin using multiphoton microscopy http://www.medworm.com/index.php?rid=5293771&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100069 This study has focused on depth profiling of sub‐resolution gold nanoparticles (AuNP) in reconstructed (fixed and unfixed) and human skin using multiphoton microscopy. Point spread functions (PSF) were determined for the used water‐immersion objective of 63×/NA = 1.2. Factors such as skin‐tissue compactness and the presence of wrinkles were found to deteriorate the accuracy of depth profiling. A broad range of AuNP detectable depths (20–100 μm) in reconstructed skin was observed. AuNP could only be detected up to ∼14 μm depth in human skin. Lateral (0.5 ± 0.1 μm) and axial (1.0 ± 0.3 μm) PSF in reconstructed and human specimens were determined. Skin cells and intercellular components didn't degrade the PSF with depth. In summary, the imaging parameters of multiphoton micro... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5293771 Thu, 06 Oct 2011 04:00:00 +0100 5293771 Membrane composition of jetted lipid vesicles: a Raman spectroscopy study http://www.medworm.com/index.php?rid=5293772&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100058 AbstractMicrofluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells. (© 2011 WILEY‐VCH Verlag GmbH &... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5293772 Wed, 05 Oct 2011 04:00:00 +0100 5293772 New application of optical agent to image angiogenesis in hindlimb ischemia http://www.medworm.com/index.php?rid=5355796&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100038 AbstractOptical agents targeting αvβ3 are potential tools to image the angiogenic response to limb ischemia. The left (L) femoral artery was ligated in 17 mice and sham surgery performed on the contralateral right (R) hindlimb. Seven days later, IntegriSense (2 nmol) was injected into 11 mice and 6 were probe controls. Six hours later, mice underwent optical imaging. Ratios of photon flux in the L/R limbs were calculated. Tissue was stained for αv, CD31, and lectin. The signal was increased in the ischemic limbs compared to contralateral legs and ratio of photon flux in L/R limb averaged 2.37. Control probe showed no hindlimb signal. IntegriSense colocalized with CD 31 by dual fluorescent staining. Ratios for L/R hindlimbs correlated with quantitative lectin staining (r = 0.88, p = 0.00... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5355796 Sat, 01 Oct 2011 04:00:00 +0100 5355796 Contrast improvement in scattered light confocal imaging of skin birefringent structures by depolarization detection http://www.medworm.com/index.php?rid=5343811&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100063 AbstractHere we describe a method for enhancing the contrast in imaging skin birefringent structures. The method relies on polarization‐dependent optical properties and is implemented using cross polarized confocal microscopy. The experimental data obtained using ex‐vivo and in‐vivo measurements on human scalp hairs and human skin demonstrate a significant dependence of the change in polarization of light that interacted with the birefringent hair on the orientation of the incident polarization. The polarization dependent contrast, defined as the ratio of intensity measured for different orientations of the incident polarization when observed using cross polarized confocal microscopy furthermore depends on the hair type/degree of pigmentation and on the focusing depth inside the hair... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5343811 Sat, 01 Oct 2011 04:00:00 +0100 5343811 Hyperspectral imaging microscopy for identification and quantitative analysis of fluorescently‐labeled cells in highly autofluorescent tissue http://www.medworm.com/index.php?rid=5311232&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100066 AbstractStandard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in tissues due to high autofluorescence. Here we describe the use of hyperspectral analysis techniques to resolve and quantify fluorescently labeled cells in highly autofluorescent lung tissue. This approach allowed accurate detection of green fluorescent protein (GFP) emission spectra, even when GFP intensity was as little as 15% of the autofluorescence intensity. GFP‐expressing cells were readily quantified with zero false positives detected. In contrast, when the same images were analyzed using stan... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5311232 Sat, 01 Oct 2011 04:00:00 +0100 5311232 A review of in‐vivo optical properties of human tissues and its impact on PDT http://www.medworm.com/index.php?rid=5293770&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100062 AbstractA thorough understanding of optical properties of biological tissues is critical to effective treatment planning for therapies such as photodynamic therapy (PDT). In the last two decades, new technologies, such as broadband diffuse spectroscopy, have been developed to obtain in vivo data in humans that was not possible before. We found that the in vivo optical properties generally vary in the ranges μa = 0.03–1.6 cm–1 and μs′ = 1.2–40 cm–1, although the actual range is tissue‐type dependent. We have also examined the overall trend of the absorption spectra (for μa and μs′) as a function of wavelength within a 95% confidence interval for various tissues in vivo. The impact of optical properties on light fluence rate is also discussed for various light application g... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5293770 Sat, 01 Oct 2011 04:00:00 +0100 5293770 Editorial: Clinical Biophotonics http://www.medworm.com/index.php?rid=5269444&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100510 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5269444 Sat, 01 Oct 2011 00:37:50 +0100 5269444 Contents: J. Biophotonics 10/2011 http://www.medworm.com/index.php?rid=5269443&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100509 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5269443 Sat, 01 Oct 2011 00:37:49 +0100 5269443 Issue Information: J. Biophotonics 10/2011 http://www.medworm.com/index.php?rid=5269442&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190013 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5269442 Sat, 01 Oct 2011 00:37:47 +0100 5269442 Cover Picture: J. Biophotonics 10/2011 http://www.medworm.com/index.php?rid=5269441&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190012 AbstractSurface Enhanced Raman Spectroscopy or SERS has come a long way since its discovery in the 70's. The recent advances in the nanotechnology have brought SERS closer to the clinical field. Images shown represent a variety of SERS‐active nanostructures and their preclinical and clinical applications that will be highlighted in the review article by Kho, Fu, Dinish, and Olivo. (Picture: Kho et al., pp. 667–684 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5269441 Sat, 01 Oct 2011 00:37:46 +0100 5269441 Raman spectroscopy for the non‐contact and non‐destructive monitoring of collagen damage within tissues http://www.medworm.com/index.php?rid=5269440&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100068 In conclusion, our data demonstrate that Raman microscopy is a promising, non‐destructive and non‐contact tool to probe ECM state in situ. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5269440 Sat, 01 Oct 2011 00:37:37 +0100 5269440 Detection of familial adenomatous polyposis with orthogonal polarized spectroscopy of the oral mucosa vasculature http://www.medworm.com/index.php?rid=5233913&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100057 AbstractFamilial Adenomatous Polyposis (FAP) is an autosomal dominant disease characterized by the development of multiple colonic polyps at younger age with a near 100% lifetime risk of colorectal cancer. The determination of FAP is made after extensive clinical evaluation and genetic testing of at risk individuals. We investigated a novel spectro‐polarimetric imaging system capable of capturing high‐resolution images of the oral mucosa at different wavelengths in an attempt to distinguish patients with FAP from controls. Results of a clinical trial show that the system is capable of separating FAP positive individuals from controls by measuring the individuals' oral vascular density and complexity. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophot... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5233913 Thu, 15 Sep 2011 04:00:00 +0100 5233913 Clinical SERS: are we there yet? http://www.medworm.com/index.php?rid=5233912&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100047 This article provides an overview of SERS and its applicability in the field of bio‐medicine. We will preview recent developments in SERS substrate designs, and the various sensing technologies that are based on the SERS phenomenon. An overview of the clinical applications of SERS is also included. Finally, we provide an opinion on the future trends of this unique spectroscopic technique. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5233912 Thu, 01 Sep 2011 04:00:00 +0100 5233912 Comparative evaluation of the diagnostic performance of autofluorescence and diffuse reflectance in oral cancer detection: a clinical study http://www.medworm.com/index.php?rid=5204550&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100037 AbstractAutofluorescence (AF) and diffuse reflectance (DR) spectroscopic techniques have shown good diagnostic accuracies for noninvasive detection of oral cavity cancer. In the present study, AF and DR spectra recorded in vivo from the same set of sites in 65 patients were analyzed using Principal component analysis (PCA) and linear discriminant analysis (LDA). The effectiveness of these two techniques was assessed by comparison with gold standard and their discrimination efficiency was determined from the area under the receiver operator characteristic (AUC‐ROC) curve. Analysis using a DR technique shows a higher AUC‐ROC of 0.991 as against 0.987 for AF spectral data. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5204550 Thu, 01 Sep 2011 04:00:00 +0100 5204550 Light‐guided lumpectomy: first clinical experience http://www.medworm.com/index.php?rid=5191489&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100054 We report on the early use of light‐guided lumpectomy. Eight patients with non‐palpable breast cancer undergoing lumpectomy for biopsy‐proven and radiographically identifiable cancer were enrolled in the study. An optical wire was designed that incorporated a standard hook‐wire with an optical fiber. The optical wire was placed in the same manner as a standard hook‐wire. During light‐guided lumpectomy, an eye‐safe laser illuminated the optical wire and created a sphere of light surrounding the cancer. The light was visible at the beginning of each surgery and facilitated approaching the cancer without using the wire. Dissection around the sphere of light kept the wire tip within the surgical specimen. Three of eight initial surgical specimens had focally positive margins. Add... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5191489 Wed, 31 Aug 2011 23:00:00 +0100 5191489 Femtosecond laser nanosurgery of sub‐cellular structures in HeLa cells by employing Third Harmonic Generation imaging modality as diagnostic tool http://www.medworm.com/index.php?rid=5169044&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100055 AbstractFemtosecond laser assisted nanosurgery of microscopic biological specimens is a relatively new technique which allows the selective disruption of sub‐cellular structures without causing any undesirable damage to the surrounding regions. The targeted structures have to be stained in order to be clearly visualized for the nanosurgery procedure. However, the validation of the final nanosurgery result is difficult, since the targeted structure could be simply photobleached rather than selectively destroyed. This fact comprises a main drawback of this technique. In our study we employed a multimodal system which integrates non‐linear imaging modalities with nanosurgery capabilities, for the selective disruption of sub‐cellular structures in HeLa cancer cells. Third Harmonic Genera... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5169044 Sun, 28 Aug 2011 03:05:18 +0100 5169044 Contents: J. Biophotonics 9/2011 http://www.medworm.com/index.php?rid=5156143&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100508 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5156143 Thu, 25 Aug 2011 09:31:38 +0100 5156143 Issue Information: J. Biophotonics 9/2011 http://www.medworm.com/index.php?rid=5156142&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190011 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5156142 Thu, 25 Aug 2011 09:31:37 +0100 5156142 Cover Picture: J. Biophotonics 9/2011 http://www.medworm.com/index.php?rid=5156141&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190010 AbstractAn example of Pallasea cancelloides – benthic Baikal amphipod employed for the studies of stress conditions. (Picture: D.V. Axenov‐Gribanov et al., pp. 619–626 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5156141 Thu, 25 Aug 2011 09:31:36 +0100 5156141 Clinical feasibility of monitoring m‐THPC mediated photodynamic therapy by means of fluorescence differential path‐length spectroscopy http://www.medworm.com/index.php?rid=5156140&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100051 This study demonstrates the feasibility of monitoring clinical photodynamic therapy treatments using optical spectroscopy. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5156140 Thu, 25 Aug 2011 09:31:30 +0100 5156140 In vivo measurement of bladder wall oxygen saturation using optical spectroscopy http://www.medworm.com/index.php?rid=5130084&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100043 AbstractCurrent diagnosis, follow‐up and treatment of patients suffering from bladder dysfunction are mainly symptom‐targeted. A recently recognized cause of continuing bladder function loss is a deteriorated bladder microvasculature. Incorporating this aspect into the clinical diagnostic toolbox may improve treatment results. Recent developments in the field of optical spectroscopy now allow for non‐invasive measurement of microvascular blood oxygen saturation in living tissue. We have recently reported pre‐clinical data that show that this marker can be successfully measured in an animal bladder. In the animal model the marker differentiated bladders with loss of function from those with normal function. In the present paper, we report on the first in vivo measurement of this mar... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5130084 Sun, 14 Aug 2011 23:00:00 +0100 5130084 Multimodal biophotonic workstation for live cell analysis http://www.medworm.com/index.php?rid=5130083&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100052 AbstractA reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase‐contrast microscopy. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, W... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5130083 Sun, 14 Aug 2011 23:00:00 +0100 5130083 Monitoring blood volume and saturation using superficial fibre optic reflectance spectroscopy during PDT of actinic keratosis http://www.medworm.com/index.php?rid=5130082&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100053 This study shows that DPS allows optically monitoring the superficial blood volume and saturation during skin PDT. The patient‐specific variability supports the need for dosimetric measurements. In DPS, the lesion‐specific optimal interrogation depth can be varied based on lesion thickness. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5130082 Sun, 31 Jul 2011 23:00:00 +0100 5130082 Raman spectroscopy: a potential tool for early objective diagnosis of neoplasia in the oesophagus http://www.medworm.com/index.php?rid=5106169&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100041 AbstractThere is a profound clinical need for a diagnostic tool that will enable clinicians to identify early neoplastic change in the oesophagus. Raman Spectroscopy (RS) has demonstrated the potential to provide non‐invasive, rapid, objective diagnosis of endoscopically invisible precancerous oesophageal dysplasia in vitro. RS analyses biological material to identify highly specific biochemical information that can be used to influence clinical care. Raman spectroscopic mapping could provide automated assessment of tissue biopsies to aid histopathological diagnosis in vitro. Furthermore, the recent development of fibre‐optic Raman probes has enabled endoscopic assessment of oesophageal mucosa in vivo. Accurate identification of dysplasia will enable targeted endoscopic resection of ea... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5106169 Sun, 31 Jul 2011 23:00:00 +0100 5106169 Raman microspectroscopy of melanosomes: the effect of long term light irradiation http://www.medworm.com/index.php?rid=5076629&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100008 In this study, melanosomes were isolated from the retinal pigment epithelium cells and exposed to green light (532 nm), and the chemical changes were monitored using Raman microspectroscopy. Photochemical changes were recorded for different power levels and exposure times. The threshold power and the rate for irreversible photobleaching of melanosomes were calculated by fitting the experimental data with a proposed model. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5076629 Sat, 30 Jul 2011 02:45:22 +0100 5076629 A comparison of methods using optical coherence tomography to detect demineralized regions in teeth http://www.medworm.com/index.php?rid=5061586&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100014 AbstractOptical coherence tomography (OCT) is a three‐ dimensional optical imaging technique that can be used to identify areas of early caries formation in dental enamel. The OCT signal at 850 nm back‐reflected from sound enamel is attenuated stronger than the signal back‐reflected from demineralized regions. To quantify this observation, the OCT signal as a function of depth into the enamel (also known as the A‐scan intensity), the histogram of the A‐scan intensities and three summary parameters derived from the A‐scan are defined and their diagnostic potential compared. A total of 754 OCT A‐scans were analyzed. The three summary parameters derived from the A‐scans, the OCT attenuation coefficient as well as the mean and standard deviation of the lognormal fit to the hist... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5061586 Mon, 25 Jul 2011 19:58:34 +0100 5061586 Contents: J. Biophotonics 7–8/2011 http://www.medworm.com/index.php?rid=5048617&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100507 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5048617 Fri, 22 Jul 2011 00:27:50 +0100 5048617 Issue Information: J. Biophotonics 7–8/2011 http://www.medworm.com/index.php?rid=5048616&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190009 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5048616 Fri, 22 Jul 2011 00:27:49 +0100 5048616 Cover Picture: J. Biophotonics 7–8/2011 http://www.medworm.com/index.php?rid=5048615&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190008 AbstractFuture multi‐spectral dual axes confocal endomicroscope designed with MEMS mirror and piezoelectric actuator for real‐time vertical cross‐sectional imaging of specific molecular probes. (Picture: Zhongyao Liu and Zhen Qiu, see also S. F. Elahi and T. D. Wang, pp. 471–481, in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5048615 Fri, 22 Jul 2011 00:27:49 +0100 5048615 Oxygen‐dependent delayed fluorescence measured in skin after topical application of 5‐aminolevulinic acid http://www.medworm.com/index.php?rid=5048613&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100040 AbstractMitochondrial oxygen tension can be measured in vivo by means of oxygen‐dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5‐aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than mono‐exponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotoni... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5048613 Fri, 22 Jul 2011 00:27:42 +0100 5048613 A fluorescence lifetime spectroscopy study of matrix metalloproteinases‐2 and ‐9 in human atherosclerotic plaque http://www.medworm.com/index.php?rid=5048614&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100042 This study aims to determine whether MMP‐2 and ‐9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time‐resolved laser‐induced fluorescence spectroscopy (TR‐LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP‐2 and ‐9 content in the atherosclerotic plaque cap using a label‐free imaging technique implemented with a fiberoptic TR‐LIFS system. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5048614 Mon, 18 Jul 2011 23:00:00 +0100 5048614 Quantitative remineralization evolution kinetics of artificially demineralized human enamel using photothermal radiometry and modulated luminescence http://www.medworm.com/index.php?rid=5035608&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100026 AbstractHuman molars were subjected to demineralization in acid gel followed by incubation in remineralization solutions without or with fluoride (1 or 1000 ppm). Photothermal radiometry (PTR) and modulated luminescence (LUM) frequency scans were performed prior to and during de/remineralization treatments. Transverse Micro‐Radiography (TMR) analysis followed at treatment conclusion to determine mineral loss and lesion depth. The remineralization process illustrated a complex interplay between surface and subsurface mineral deposition, confining the thermal‐wave centroid toward the dominating layer. Experimental amplitudes and phases were fitted to a coupled diffuse‐photon‐density‐wave and thermal‐wave theoretical model used to quantitatively evaluate evolving changes in therma... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5035608 Tue, 31 May 2011 23:00:00 +0100 5035608 Future and advances in endoscopy http://www.medworm.com/index.php?rid=5027075&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100048 AbstractThe future of endoscopy will be dictated by rapid technological advances in the development of light sources, optical fibers, and miniature scanners that will allow for images to be collected in multiple spectral regimes, with greater tissue penetration, and in three dimensions. These engineering breakthroughs will be integrated with novel molecular probes that are highly specific for unique proteins to target diseased tissues. Applications include early cancer detection by imaging molecular changes that occur before gross morphological abnormalities, personalized medicine by visualizing molecular targets specific to individual patients, and image guided therapy by localizing tumor margins and monitoring for recurrence. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (So... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=5027075 Tue, 31 May 2011 23:00:00 +0100 5027075 In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach http://www.medworm.com/index.php?rid=4974846&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100033 We present a multi‐modal optical diagnostic approach utilizing a combined use of Fluorescence Intravital Microscopy (FIM), Dynamic Light Scattering (DLS) and Spectrally Enhanced Microscopy (SEM) modalities for in vivo imaging of tumor vascular network and blood microcirculation. FIM is used for imaging of tumor surroundings and microenvironment, SEM provides information regarding blood vessels topography, whereas DLS is applied for functional imaging of vascular network and blood microcirculation. This complementary combination of the imaging approaches is extremely useful for functional in vivo imaging of blood vasculature and tumor microenvironment. The technique has also a great potential in vascular biology and can significantly expand the capabilities of tumor angiogenesis studies a... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4974846 Tue, 31 May 2011 23:00:00 +0100 4974846 Spectroscopic detection of β ‐sheet structure in nascent Aβ oligomers http://www.medworm.com/index.php?rid=4967779&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100023 AbstractDeep‐UV resonance Raman (UVRR) spectroscopy and circular dichroism (CD) were employed to study the secondary structure of Aβ(1–42) in fresh samples with increasing fractions of oligomeric peptide. A feature with a minimum at ∼217 nm appeared in CD spectra of samples containing oligomeric Aβ(1–42). UVRR spectra more closely resembled those of disordered proteins. The primary difference between UVRR spectra was the ratio of the 1236 cm–1 to 1260 cm–1 amide III peak intensities, which shifted in favor of the 1236 cm–1 band as the fraction of oligomeric peptide increased. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4967779 Tue, 31 May 2011 23:00:00 +0100 4967779 NIR spectroscopic imaging to map hemoglobin + myoglobin oxygenation, their concentration and optical pathlength across a beating pig heart during surgery http://www.medworm.com/index.php?rid=4951633&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100031 AbstractThe purpose of this paper is to demonstrate that near‐infrared (NIR) spectroscopic imaging can provide spatial distribution (maps) of the absolute concentration of hemoglobin + myoglobin, oxygen saturation parameter and optical pathlength, reporting on the biochemico‐physiological status of a beating heart in vivo. The method is based on processing the NIR spectroscopic images employing a first‐derivative approach. Blood‐pressure‐controlled gating compensated the effect of heart motion on the imaging. All the maps are available simultaneously and noninvasively at a spatial resolution in the submillimeter range and can be obtained in a couple of minutes. The equipment has no mechanical contact with the tissue, thereby leaving the heart unaffected during the measurement. (... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4951633 Tue, 31 May 2011 23:00:00 +0100 4951633 An unmet medical need: Advances in endoscopic imaging of colorectal neoplasia http://www.medworm.com/index.php?rid=4932642&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100027 AbstractGastrointestinal cancer is a major public health problem worldwide. Detection of early neoplastic lesions in gastrointestinal tract is essential for cure, because prognosis and survival are related to the size and stage of malignant lesions. Endoscopic screening and treatment of polyps could prevent approximately 80% of colorectal cancer (CRC). However, white‐light endoscopy is an imperfect technology since miss rates of up to 25% have been reported and polyps without malignant potential were treated without benefit but with additional costs and risks to the patient. There are several known “human” predictors of an inadequate colonoscopy. These include patient characteristics such as poor bowel preparation, female gender, or inpatient status. Skills of the endoscopists are al... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4932642 Tue, 31 May 2011 23:00:00 +0100 4932642 Raman spectroscopic analysis of the carotenoid concentration in egg yolks depending on the feeding and housing conditions of the laying hens http://www.medworm.com/index.php?rid=4920246&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100036 AbstractResonance Raman spectroscopic measurements are suited to analyze the concentration of carotenoid antioxidants in biological samples. Previously, it has been shown that the carotenoid concentration of nutritional egg yolks has a direct influence on the carotenoid content of human skin in vivo. In the present study, resonance Raman spectroscopy was used to analyze the carotenoid concentration in the yolks of hen eggs, which were housed in battery cages or alternatively on free‐range grassland. The egg yolks of hens, which had access to grassland, contained approximately double the amount of carotenoid concentration than the egg yolks of hens housed in battery cages (p < 0.001). The kinetics of the carotenoid concentration in the egg yolks, depending on fodder, housing and weathe... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4920246 Tue, 31 May 2011 23:00:00 +0100 4920246 Advanced markers and labels for life science and biomedical applications http://www.medworm.com/index.php?rid=4861482&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100506 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4861482 Wed, 25 May 2011 23:42:22 +0100 4861482 Contents: J. Biophotonics 6/2011 http://www.medworm.com/index.php?rid=4861481&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100505 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4861481 Wed, 25 May 2011 23:42:16 +0100 4861481 Issue Information: J. Biophotonics 6/2011 http://www.medworm.com/index.php?rid=4861480&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190007 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4861480 Wed, 25 May 2011 23:42:13 +0100 4861480 Cover Picture: J. Biophotonics 6/2011 http://www.medworm.com/index.php?rid=4861479&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190006 AbstractApplication of green‐to‐red photoconvertible EosFP and its variants in live‐cell experiments. Cell fate mapping using EosFP injected into developing Xenopus embryos (first row). Protein tracking at 37 °C using the tubulinbinding protein RITA fused to mEosFPthermo (second row). Pulse‐chase experiment with super‐resolution using photoconvertible and photoswitchable mIrisFP (third row). Assembly of a focal adhesion imaged with super‐resolution using paxillin‐mIrisFP (bottom row). (Picture: for the complete figure and credits see J. Wiedenmann et al., pp. 377–390 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4861479 Wed, 25 May 2011 23:42:12 +0100 4861479 How histological features of basal cell carcinomas influence image quality in optical coherence tomography http://www.medworm.com/index.php?rid=4839957&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100006 AbstractOptical coherence tomography (OCT) has the potential to diagnose and measure the depth of nonmelanoma skin cancer (NMSC) in skin, but some lesions appear blurred in OCT images. The aim of this study is to identify histological characteristics of basal cell carcinomas (BCC) that correlate with good quality OCT images of the same lesions. A total of 34 patients with BCC were OCT scanned. The influence of histology parameters (e.g. inflammation, sun damage of skin, carcinoma cell size) on OCT image quality was studied by comparing 15 BCC lesions easily identified compared to 19 BCC lesions that produced only blurred in OCT images. Inflammation was more pronounced in blurred OCT images, whereas solar elastosis dominated in easily identified lesions. Hyperkeratosis did not impair imagin... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4839957 Wed, 18 May 2011 23:00:00 +0100 4839957 Can color inhomogeneity of bruises be used to establish their age? http://www.medworm.com/index.php?rid=4839956&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100021 In conclusion, color inhomogeneity of bruises can be used to determine their age. We expect that future age determination of bruises by the inverse procedure described here, possibly also including the distribution of concentrations in the areas will open up a new phase in clinical bruise classification. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4839956 Wed, 18 May 2011 23:00:00 +0100 4839956 Optical biosensors to analyze novel biomarkers in oncology http://www.medworm.com/index.php?rid=4819877&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000123 AbstractMany cancer types are characterized by poor survival and unpredictable therapy response. Easy‐to‐perform molecular analyses may help patient stratification and treatment tailoring. Several integrated devices have been proposed to overcome current analysis equipment limitations. They offer improved sensitivity and easy availability of parallel detection. Particularly, unlabelled optical biosensors combine the manifold advantages of integrated sensors (e.g. easy handling, portability and low‐volume requirement) with detection of target molecules in their original form. Here, we review integrated optical biosensor current features, and discuss their possible application to the detection of protein variants from body fluids, with particular regard to histone modifications. Indeed... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4819877 Thu, 12 May 2011 23:00:00 +0100 4819877 Chemical tags: Applications in live cell fluorescence imaging http://www.medworm.com/index.php?rid=4819878&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100018 AbstractTechnologies to visualize cellular structures and dynamics enable cell biologists to gain insight into complex biological processes. Currently, fluorescent proteins are used routinely to investigate the behavior of proteins in live cells. Chemical biology techniques for selective labeling of proteins with fluorescent labels have become an attractive alternative to fluorescent protein labeling. In the last ten years the progress in the development of chemical tagging methods have been substantial offering a broad palette of applications for live cell fluorescent microscopy. Several methods for protein labeling have been established, using protein tags, peptide tags and enzyme mediated tagging. This review focuses on the different strategies to achieve the attachment of fluorophores ... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4819878 Wed, 11 May 2011 23:00:00 +0100 4819878 Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin http://www.medworm.com/index.php?rid=4839955&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100028 AbstractAll‐optical microspectroscopic and tomographic tools have a great potential for the clinical investigation of human skin and skin diseases. However, automated optical tomography or even microscopy generate immense data sets. Therefore, in order to implement such diagnostic tools into the medical practice in both hospitals and private practice, there is a need for automated data handling and image analysis ideally implementing automized scores to judge the physiological state of a tissue section. In this contribution, the potential of an image processing algorithm for the automated classification of skin into normal or keloid based on second‐harmonic generation (SHG) microscopic images is demonstrated. Such SHG data is routinely recorded within a multimodal imaging approach. The... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4839955 Sat, 30 Apr 2011 23:00:00 +0100 4839955 Confocal laser endomicroscopy in gastrointestinal diseases http://www.medworm.com/index.php?rid=4819876&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100022 AbstractConfocal laser endomicroscopy (CLE) is a novel endoscopic technique permitting in vivo microscopy (optical biopsies) of the gastrointestinal mucosa. CLE has been studied in a multitude of diseases of the upper and lower gastrointestinal tract, including Barrett's esophagus, gastric inflammation and cancer, celiac disease, colorectal adenoma and carcinoma, and inflammatory bowel diseases. CLE has recently evolved and been studied for bile duct and liver imaging. CLE has shown overall high accuracy and enabled smart, targeted biopsies rather than untargeted sampling. Furthermore, the availability of real time microscopic information during endoscopy has immediate impact on therapeutic decisions and guides endoscopic interventions. CLE is also a unique tool for observation of (patho... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4819876 Sat, 30 Apr 2011 23:00:00 +0100 4819876 Autofluorescence imaging of early colorectal cancer http://www.medworm.com/index.php?rid=4812241&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100013 AbstractAutofluorescence imaging (AFI) is expected to better detect colorectal neoplasms than the conventional white light imaging (WLI). The AFI image of colorectal cancer is different according to its macroscopic type. Protruded lesion, which is the most common macroscopic type of colorectal neoplasms, is revealed as magenta protruded lesion. Flat lesion, which is uncommon macroscopic type, is presented as distinct magenta area surrounding green mucosa using AFI. And depressed lesion, which is rare macroscopic type, is revealed as green area surrounded by magenta mucosa. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4812241 Sat, 30 Apr 2011 23:00:00 +0100 4812241 Optical diagnostic test of stress conditions of aquatic organisms http://www.medworm.com/index.php?rid=4788302&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100025 AbstractGlobal climate change has become a dire reality and its impact is expected to rise dramatically in the near future. Combined with the day‐to‐day human activities the climatic changes heavily affect the environment. In particular, a global temperature increase accompanied by a number of anthropogenic chemicals falling within the freshwater ecosystem results in a dramatic enhancement of the overall stress for most aquatic organisms. This leads to a significant shift in the species inventory and potential breakdown of the water ecosystem with severe consequences for local economies and water supply. In order to understand and predict the influence of climatic changes on the physiological and biochemical processes that take place in living aquatic organisms we explore the applicati... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4788302 Sat, 30 Apr 2011 23:00:00 +0100 4788302 Observations of cardiac beating behaviors of wild‐type and mutant Drosophilae with optical coherence tomography http://www.medworm.com/index.php?rid=4779870&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100009 AbstractTime‐resolved optical coherence tomography (OCT) scanning images of wild‐type and mutant fruit flies (Drosophila melanogaster), illustrating the heartbeat patterns for evaluating their cardiac functions, are demonstrated. Based on the heartbeat patterns, the beat rate and the relative phase between the first two heart segments can be evaluated. The OCT scanning results of mutant flies with impaired proteasome function in cardiac muscles show irregular heartbeat patterns and systematically decreased average beat rates, when compared with the regular patterns of ∼4.97 beats/s in average beat rate of the wild‐type. In both wild‐type and proteasome mutant flies, the beatings at different locations in the same heart segment are essentially synchronized. However, between differ... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4779870 Sat, 30 Apr 2011 23:00:00 +0100 4779870 Contents: J. Biophotonics 5/2011 http://www.medworm.com/index.php?rid=4732896&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100504 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4732896 Thu, 21 Apr 2011 14:29:59 +0100 4732896 Issue Information: J. Biophotonics 5/2011 http://www.medworm.com/index.php?rid=4732895&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190005 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4732895 Thu, 21 Apr 2011 14:29:59 +0100 4732895 Cover Picture: J. Biophotonics 5/2011 http://www.medworm.com/index.php?rid=4732894&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190004 AbstractSecond harmonic generation (green) and two‐photon autofluorescence (red) allow simultaneous visualization of the collagen and myocyte components of stem‐cell treated infarcted rat myocardium. (Picture: M. A. Wallenburg et al., pp. 297–304 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4732894 Thu, 21 Apr 2011 14:29:58 +0100 4732894 Quantitative analysis of shape and volume changes in activated thrombocytes in real time by single‐shot spatial light modulator‐based differential interference contrast imaging http://www.medworm.com/index.php?rid=4723109&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100010 AbstractWe suggest to use a combination of optical tweezers and single‐image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially designed phase mask displayed at the SLM enables quantitative phase calculation from only a single recording. The optical tweezers stabilize trapped thrombocytes for long‐time monitoring of changes in the optical thickness profile of thrombocytes during activation by adenosine diphosphate (ADP). (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4723109 Thu, 31 Mar 2011 23:00:00 +0100 4723109 Nanometer‐scale imaging by the modulation tracking method http://www.medworm.com/index.php?rid=4669687&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100002 AbstractWe developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer‐resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured opossum kidney cells expressing NaPi co‐transporter proteins with different GFP constructs and images of cell protrusions in a collagen matrix with a resolution of about 20 nm. We found that in the microvilli the NaPi proteins can be found clustered. Along cell protrusions in 3D we identified cellular adhesions to the extracellular matrix. Our approach to super‐resolution and to 3D nanoimaging is different than other proposed methods that break the diffraction li... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4669687 Thu, 31 Mar 2011 23:00:00 +0100 4669687 Monitoring temporal development and healing of diabetic foot ulceration using hyperspectral imaging http://www.medworm.com/index.php?rid=4663400&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000117 This study combines non‐invasive hyperspectral imaging with an experimentally validated skin optical model and inverse algorithm to monitor diabetic feet of two representative patients. It aims to observe temporal changes in local epidermal thickness and oxyhemoglobin concentration and to gain insight into the progression of foot ulcer formation and healing. Foot ulceration is a debilitating comorbidity of diabetes that may result in loss of mobility and amputation. Inflammation and necrosis preempt ulceration and can result in changes in the skin prior to ulceration and during ulcer healing that affect oxygen delivery and consumption. Previous studies estimated oxyhemoglobin and deoxyhemoglobin concentrations around pre‐ulcerative and ulcer sites on the diabetic foot using commerciall... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4663400 Thu, 31 Mar 2011 23:00:00 +0100 4663400 Contents: J. Biophotonics 4/2011 http://www.medworm.com/index.php?rid=4652190&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100503 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4652190 Wed, 30 Mar 2011 04:03:33 +0100 4652190 Issue Information: J. Biophotonics 4/2011 http://www.medworm.com/index.php?rid=4652189&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190003 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4652189 Wed, 30 Mar 2011 04:03:32 +0100 4652189 Cover Picture: J. Biophotonics 4/2011 http://www.medworm.com/index.php?rid=4652188&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190002 AbstractFluorescence images of two sides of slabs from pig hearts. Photographs of the samples are above the images. Excitation, 635 nm.Dotted lines contour the areas in which the intensities at each pixel were averaged and the plotted versus the FM concentration. (Picture: E. Gussakowsky et al., pp. 277–287 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4652188 Wed, 30 Mar 2011 04:03:32 +0100 4652188 Fast and long term lipid droplet tracking with CARS microscopy http://www.medworm.com/index.php?rid=4641899&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000120 AbstractPhotobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label‐free imaging techniques therefore offer advantages both for rapid image acquisition and for long–term observations. CARS microscopy is a label‐free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video‐rate tracking of intracellular transport of lipid droplets, but also continuous long‐term observation of cells over several hours. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4641899 Tue, 01 Mar 2011 00:00:00 +0100 4641899 Enhancement of imaging depth in turbid media using a wide area detector http://www.medworm.com/index.php?rid=4610323&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100001 AbstractThe depth of two‐photon fluorescence imaging in turbid media can be significantly enhanced by the use of the here described fluorescence detection method that allows to efficiently collect scattered fluorescence photons from a wide area of the turbid sample. By using this detector we were able to perform imaging of turbid samples, simulating brain tissue, at depths up to 3 mm, where the two‐photon induced fluorescence signal is too weak to be detected by means used in conventional two‐photon microscopy. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4610323 Tue, 01 Mar 2011 00:00:00 +0100 4610323 Multimicroscopic study of curcumin effect on fixed nonmalignant and cancerous mammalian epithelial cells http://www.medworm.com/index.php?rid=4570136&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000119 AbstractThe morphology changes, in particular the organization of microtubules in mammalian nonmalignant HMEC 184A1 and cancerous MCF‐7 cells during curcumin treatment have been investigated by utilizing multiphoton, fluorescence, and atomic force (AFM) microscopies. Fluorescence microscopy reveals formation of ring‐like structures of microtubules circumscribing the nuclear area in HMEC 184A1 cells after treatment, while in MCF‐7 cells, no important changes were observed. Topography analyses of fixed HMEC 184A1 and MCF‐7 before and after treatment with curcumin were performed using AFM and the effect of the employed cells' fixation method was investigated on MCF‐7 cells. Due to its indepth optical sectioning capacity multiphoton microscopy provided valuable complementary informat... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4570136 Tue, 01 Mar 2011 00:00:00 +0100 4570136 Nanoscale spectroscopy and imaging of hemoglobin http://www.medworm.com/index.php?rid=4549451&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100003 AbstractSub diffraction limited infrared absorption imaging of hemoglobin was performed by coupling IR optics with an atomic force microscope. Comparisons between the AFM topography and IR absorption images of micron sized hemoglobin features are presented, along with nanoscale IR spectroscopic analysis of the metalloprotein. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4549451 Tue, 01 Mar 2011 00:00:00 +0100 4549451 Dynamic study of PLGA/CS nanoparticles delivery containing drug model into phantom tissue using CO2 laser for clinical applications http://www.medworm.com/index.php?rid=4488940&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000121 In this study, cationic nanoparticles (NPs) were prepared by coating chitosan (CS) on the surface of PLGA NPs. To our knowledge most of the work in the field of drug delivery systems using lasers has been performed using short pulses with micron and submicron durations. We carried out an experiment using superlong PLS‐R (10 ms) and CW CO2laser modes on simulated drug‐biogelatin model where drug was encapsulated by PLGA/CS NPs. Maximum depth of drug containing cavitation was achieved faster at higher powers and shorter irradiation time in CWC mode. We believe that the main mechanism at work with superlong pulses is both photothermal due to vaporization and photomechanical due to photophoresis and cavitation collapse. In the case of CW, however, it is purely photothermal. Thus, drug mole... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4488940 Fri, 18 Feb 2011 01:27:59 +0100 4488940 From EosFP to mIrisFP: structure‐based development of advanced photoactivatable marker proteins of the GFP‐family http://www.medworm.com/index.php?rid=4477096&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000122 AbstractFluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non‐bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse‐chase experiments and super‐resolution imaging. In this review, we discuss the development of advanced variants, using a structure‐function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ∼400 nm l... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4477096 Tue, 15 Feb 2011 11:09:20 +0100 4477096 Design and synthesis of Raman reporter molecules for tissue imaging by immuno‐SERS microscopy http://www.medworm.com/index.php?rid=4445332&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000116 AbstractThe design and synthesis of Raman reporter molecules comprising olefin or alkyne moieties with strong and characteristic vibrational Raman bands is presented. Chemisorption onto the surface of colloidal Au/Ag shells yields a self‐assembled monolayer. Hydrophilic stabilization of such SERS labels can be achieved by short terminal ethylene glycol units attached to the Raman reporter. Encapsulation by silica with subsequent functionalization of the glass surface allows the conjugation to biomolecules such as antibodies. We demonstrate the use of SERS‐labeled antibodies for tissue imaging of the tumor suppressor p63 in prostate biopsies. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4445332 Tue, 08 Feb 2011 00:33:40 +0100 4445332 Laser applications in life sciences http://www.medworm.com/index.php?rid=4423719&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100502 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4423719 Tue, 01 Feb 2011 23:42:16 +0100 4423719 Contents: J. Biophotonics 3/2011 http://www.medworm.com/index.php?rid=4423718&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201100501 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4423718 Tue, 01 Feb 2011 23:42:15 +0100 4423718 Issue Information: J. Biophotonics 3/2011 http://www.medworm.com/index.php?rid=4423717&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190001 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4423717 Tue, 01 Feb 2011 23:42:15 +0100 4423717 Cover Picture: J. Biophotonics 3/2011 http://www.medworm.com/index.php?rid=4423716&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201190000 AbstractThe cluster of nanobubbles of a dissolved gas adheres to the membrane of the erythrocyte. This cluster is generated due to a steep decrease of arterial pressure which results in the supersaturation of blood with respect to the dissolved gas. The supersaturation leads to the gas nanobubbles nucleation at the erythrocyte membrane. Such an effect is one of possible displays of caisson disease. (Picture: N. F. Bunkin et al., pp. 150–164 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4423716 Tue, 01 Feb 2011 23:42:14 +0100 4423716 Enhancement and optimization of plasmid expression in femtosecond optical transfection http://www.medworm.com/index.php?rid=4342494&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000105 We report here a method to achieve significant enhancement in the efficiency of femtosecond optical transfection. The transfection procedure is modified by incorporating a suitable synthetic peptide containing nuclear localization and DNA binding sequences, assisting DNA import into the nucleus. We achieved a 3‐fold enhancement in the transfection efficiency for adherent Chinese Hamster Ovary (CHO‐K1) cells with this modified protocol. Further, in the presence of this biochemical reagent, we were able to reduce the required plasmid concentration by ∼70% without compromising the transfection efficiency. Also, we report for the first time the successful photo‐transfection of recently trypsinised cells with significantly high transfection efficiency when transfected with modified plas... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4342494 Thu, 13 Jan 2011 00:00:00 +0100 4342494 Enhanced bioanalyte detection in waveguide confined Raman spectroscopy using wavelength modulation http://www.medworm.com/index.php?rid=4393719&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000107 AbstractWaveguide confined Raman spectroscopy (WCRS) incorporates a fibre based Raman detection system in a microfluidic platform enabling the spectroscopic detection of analyte. It offers the possibility to develop portable, alignment free devices for bio‐analyte sensing with minimal sample preparation. Ultimate sensitivity is limited by the fibre auto‐fluorescence background. Here we report enhanced bio‐analyte detection sensitivity by combining WCRS with continuous wavelength modulation technique. We used urea as a model analyte and the modulation parameters have been optimized to maximize the sensitivity of the device. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4393719 Sat, 01 Jan 2011 00:00:00 +0100 4393719 Interferometric fiber optic sensors for biomedical applications of optoacoustic imaging http://www.medworm.com/index.php?rid=4360394&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000096 We present a non‐metallic interferometric silica optical fiber ultrasonic wideband sensor for optoacoustic imaging applications. The ultrasonic sensitivity of this sensor has been characterized over the frequency range from 1 to 10 MHz. A comparative analysis has been carried out between this sensor and an array of piezoelectric transducers using optoacoustic signals generated from an optical absorbent embedded in a tissue mimicking phantom. Also, a two dimensional reconstructed image of the phantom using the fiber interferometric sensor is presented and compared to the image obtained using the Laser Optoacoustic Imaging System, LOIS‐64B. The feasibility of our fiber optic based sensor for wideband ultrasonic detection is demonstrated. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, W... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4360394 Sat, 01 Jan 2011 00:00:00 +0100 4360394 Artefact reduction for cell migration visualization using spectral domain optical coherence tomography http://www.medworm.com/index.php?rid=4342493&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000109 AbstractVisualization of cell migration during chemotaxis using spectral domain optical coherence tomography (OCT) requires non‐standard processing techniques. Stripe artefacts and camera noise floor present in OCT data prevent detailed computer‐assisted reconstruction and quantification of cell locomotion. Furthermore, imaging artefacts lead to unreliable results in automated texture based cell analysis.Here we characterize three pronounced artefacts that become visible when imaging sample structures with high dynamic range, e.g. cultured cells: (i) time‐varying fixed‐pattern noise; (ii) stripe artefacts generated by background estimation using tomogram averaging; (iii) image modulations due to spectral shaping. We evaluate techniques to minimize the above mentioned artefacts usin... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4342493 Sat, 01 Jan 2011 00:00:00 +0100 4342493 Reflectance measurement using digital camera and a protecting dome with built in light source http://www.medworm.com/index.php?rid=4280819&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000113 This article concentrates on designing and validating an imaging system, based on a digital camera. The proposed system can measure the reflectance of the skin with high spatial and currently four channel spectral resolution, in the range of 450 nm to 980 nm. The accuracy of the system is determined by imaging a colour checker board and comparing the obtained values with both given values and spectrometer measurements. The diffuse interreflections of both, the integrating sphere and the lighting dome of the imaging system, is compensated with a correction factor. The accuracy of the proposed system is only slightly weaker than the spectrometer. The imaging system characteristics are independent of the camera characteristics. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Sourc... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4280819 Thu, 23 Dec 2010 02:53:26 +0100 4280819 Information for Authors: J. Biophotonics 1–2/2011 http://www.medworm.com/index.php?rid=4264577&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000526 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264577 Sat, 18 Dec 2010 15:07:30 +0100 4264577 Journal of Biophotonics is growing up http://www.medworm.com/index.php?rid=4264576&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000525 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264576 Sat, 18 Dec 2010 15:07:24 +0100 4264576 Contents: J. Biophotonics 1–2/2011 http://www.medworm.com/index.php?rid=4264575&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000527 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264575 Sat, 18 Dec 2010 15:07:24 +0100 4264575 Issue Information: J. Biophotonics 1–2/2011 http://www.medworm.com/index.php?rid=4264574&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201090013 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264574 Sat, 18 Dec 2010 15:07:23 +0100 4264574 Cover Picture: J. Biophotonics 1–2/2011 http://www.medworm.com/index.php?rid=4264573&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201090012 AbstractDevelopment of the posterior lateral line in a zebrafish embryo. Upper row: embryo 30 hpf, showing the migrating primordium and a deposited proneuromast. Second row, left: higher magnification image of a primordium (right) and proneuromast (left), visualized with membrane‐tagged eGFP. Second row, right: mature neuromast with ciliated hair cells (bright fluorescence) and the surrounding support cells. Third row: embryo 48 hpf, after migration of the primordium and deposition of the neuromasts. The upper and third rows were imaged with a low‐magnification wide‐field microscope, the other images with SPIM. (Picture: J. Swoger et al., pp. 122–134 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264573 Sat, 18 Dec 2010 15:07:22 +0100 4264573 Correlation mapping method for generating microcirculation morphology from optical coherence tomography (OCT) intensity images http://www.medworm.com/index.php?rid=4264571&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000103 In this study we have successfully combined a software algorithm based on correlation statistic to reveal microcirculation morphology on OCT intensity images of a mouse brain in vivo captured trans‐cranially and through a cranial window. We were able to estimate vessel geometry at bifurcation as well as along vessel segments down‐to mean diameters of about 24 μm. Our technique has potential applications in cardiovascular‐related parameter measurements such as volumetric flow as well as in assessing vascular density of normal and diseased tissue. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264571 Sat, 18 Dec 2010 15:07:12 +0100 4264571 Photons bring light into DNA repair: The comet assay and laser microbeams for studying photogenotoxicity of drugs and ageing http://www.medworm.com/index.php?rid=4264572&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000102 AbstractThis contribution reviews recent applications of micromanipulation, by UV photons, in DNA repair and ageing research as well as in the evaluation of the phototoxicity of drugs. In some cases, micromanipulation is combined with the comet assay, a technique, which allows a direct view on DNA damages. It is shown that, in humans, the sensitivity of DNA to UV induced damage and its subsequent repair is surprisingly stable up to high age and that drugs which are usually non‐toxic induce DNA damage when irradiated in parallel by UV irradiation. Using the immune fluorescent comet assay, IFCA, a variant of the comet assay, direct comparison of the effects of ionizing 137Cs radiation with those of localized UV radiation is possible. When a laser microbeam is used to damage DNA in a cell n... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4264572 Thu, 16 Dec 2010 00:00:00 +0100 4264572 Dynamic microvascular responses with a high speed TiVi imaging system http://www.medworm.com/index.php?rid=4252609&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000112 We present results based on this system by investigating dynamic responses such as arterial pulsations both from a controlled flow model and in vivo tissue sites. We also quantify the effects of sympathetic vasomotion, a biological effect which is evident in many tissue sites, and show that the effects of arterial pulsations and vasomotion on the resulting TiVi time traces are easily determined. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4252609 Wed, 01 Dec 2010 00:00:00 +0100 4252609 Multi‐dimensional fluorescence microscopy of living cells http://www.medworm.com/index.php?rid=4189609&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000098 AbstractAn overview on fluorescence microscopy with high spatial, spectral and temporal resolution is given. In addition to 3D microscopy based on confocal, structured or single plane illumination, spectral imaging and fluorescence lifetime imaging microscopy (FLIM) are used to probe the interaction of a fluorescent molecule with its micro‐environment. Variable‐angle total internal reflection fluorescence microscopy (TIRFM) permits selective measurements of cell membranes or cell‐substrate topology in the nanometre scale and is also combined with spectral or time‐resolved detection. In addition to single cells or cell monolayers, 3‐dimensional cell cultures are of increasing importance, since they are more similar to tissue morphology and function. All methods reported are adapte... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4189609 Tue, 23 Nov 2010 04:32:58 +0100 4189609 Cross‐polarization optical coherence tomography for early bladder‐cancer detection: statistical study http://www.medworm.com/index.php?rid=4189610&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000088 AbstractThe capabilities of cross‐polarization optical coherence tomography (CP OCT) for early bladder‐cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross‐polarization and copolarization simultaneously. 116 patients with localized flat suspicious lesions in the bladder were enrolled, 360 CP OCT images were obtained and analyzed. CP OCT demonstrated sensitivity 93.7% (vs. 81.2%, <0.0001), specificity 84% (vs. 70.0%, <0.001) and accuracy 85.3% (vs. 71.5%, <0.001) in detecting flat malignant bladder lesions, which is significantly better than with the traditional OCT. Higher diagnostic efficacy of CP OCT in detecting early bladder can... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4189610 Mon, 22 Nov 2010 00:00:00 +0100 4189610 Information for Authors: J. Biophoton. 12/2010 http://www.medworm.com/index.php?rid=4171547&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000524 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4171547 Tue, 16 Nov 2010 21:05:42 +0100 4171547 Optical bioimaging and neuroimaging: from whole‐body inspection to brain sensing http://www.medworm.com/index.php?rid=4171546&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000523 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4171546 Tue, 16 Nov 2010 21:05:38 +0100 4171546 Contents: J. Biophoton. 12/2010 http://www.medworm.com/index.php?rid=4171545&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000522 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4171545 Tue, 16 Nov 2010 21:05:38 +0100 4171545 Cover Picture: J. Biophoton. 12/2010 http://www.medworm.com/index.php?rid=4171544&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201090011 AbstractMultiphoton tomography provides optical biopsies with high subcellular submicron resolution based on twophoton excited autofluorescence and second harmonic generation. Even single intratissue mitochondria can be imaged. (Picture: K. König, pp. 759–773 in this issue) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4171544 Tue, 16 Nov 2010 21:05:37 +0100 4171544 Wavelength swept amplified spontaneous emission source for high speed retinal optical coherence tomography at 1060 nm http://www.medworm.com/index.php?rid=4158485&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000104 AbstractThe wavelength swept amplified spontaneous emission (ASE) source presented in this paper is an alternative approach to realize a light source for high speed swept source optical coherence tomography (OCT). ASE alternately passes a cascade of different optical gain elements and tunable optical bandpass filters. In this work we show for the first time a wavelength swept ASE source in the 1060 nm wavelength range, enabling high speed retinal OCT imaging. We demonstrate ultra‐rapid retinal OCT at a line rate of 170 kHz, a record sweep rate at 1060 nm of 340 kHz with 70 nm full sweep width, enabling an axial resolution of 11 μm. Two different implementations of the source are characterized and compared to each other. The last gain element is either a semiconductor optical amplifier o... Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4158485 Fri, 12 Nov 2010 00:00:00 +0100 4158485 Characterisation of DNA methylation status using spectroscopy (mid‐IR versus Raman) with multivariate analysis http://www.medworm.com/index.php?rid=4117328&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000085 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4117328 Wed, 27 Oct 2010 23:00:00 +0100 4117328 Lifetime imaging of FRET between red fluorescent proteins http://www.medworm.com/index.php?rid=4035919&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000065 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4035919 Mon, 04 Oct 2010 23:00:00 +0100 4035919 Light‐induced effects on Brownian displacements http://www.medworm.com/index.php?rid=4158484&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000097 AbstractEarlier work on particles in aqueous solution indicated that particle hydration could be expanded by incident light. To assess the effects of expanded hydration we measured Brownian displacements of microspheres exposed to light of varying intensities and wavelengths. Displacements were consistently diminished in an intensity‐dependent and wavelength‐dependent fashion, and center‐to‐center distances between microspheres were shifted to higher values. We conclude that suspended microspheres are surrounded by hydration zones substantial enough to impact Brownian displacements. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4158484 Fri, 01 Oct 2010 00:00:00 +0100 4158484 Long‐living nanobubbles of dissolved gas in aqueous solutions of salts and erythrocyte suspensions http://www.medworm.com/index.php?rid=4150141&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000093 AbstractResults of experiments combining laser modulation interference microscopy and Mueller matrix scatterometry show that macroscopic scatterers of light are present in liquids free of external solid impurities. Experimental data on distilled water and aqueous NaCl solutions of various concentrations as well as physiological saline solution are reported. The experimental data can be interpreted by using a model of micron‐scale clusters composed of polydisperse air nanobubbles having effective radii of 70–100 nm. Their concentration increases with the growth of ionic content. We hypothesize that under certain conditions those clusters of nanobubbles can affect the erythrocyte structure. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4150141 Fri, 01 Oct 2010 00:00:00 +0100 4150141 Ultrafast electron transfer processes studied by pump‐repump‐probe spectroscopy http://www.medworm.com/index.php?rid=4117327&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000099 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4117327 Thu, 30 Sep 2010 23:00:00 +0100 4117327 Comparison of pulsed photothermal radiometry, optical coherence tomography and ultrasound for melanoma thickness measurement in PDMS tissue phantoms http://www.medworm.com/index.php?rid=4073217&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000078 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4073217 Thu, 30 Sep 2010 23:00:00 +0100 4073217 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies http://www.medworm.com/index.php?rid=4035918&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000087 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4035918 Thu, 30 Sep 2010 23:00:00 +0100 4035918 In vivo study of the effect of mechanical compression on formation of OCT images of human skin http://www.medworm.com/index.php?rid=4011929&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000063 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4011929 Wed, 29 Sep 2010 21:08:10 +0100 4011929 Label‐free imaging and analysis of the effects of lipolysis products on primary hepatocytes http://www.medworm.com/index.php?rid=4011931&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000086 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4011931 Sun, 26 Sep 2010 23:00:00 +0100 4011931 Aqueous phase synthesis of CdTe quantum dots for biophotonics http://www.medworm.com/index.php?rid=4011930&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000080 Abstract (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=4011930 Sun, 26 Sep 2010 23:00:00 +0100 4011930 Nanobiophotonics: photons that shine their light on the life at the nanoscale http://www.medworm.com/index.php?rid=3976129&cid=s_36807_75_f&fid=36807&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjbio.201000521 (Source: Journal of Biophotonics) Journal of Biophotonics journals http://www.medworm.com/rss/comments.php?id=3976129 Fri, 17 Sep 2010 08:59:08 +0100 3976129