MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Bone and Mineral Metabolism' source. Thu, 09 Feb 2012 00:02:30 +0100 http://www.medworm.com/index.php?rid=5599813&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl1q7634641503212%2F We describe the case of a 62-year-old woman with a 2-year history of asthenia and mental depression. Her past medical history was significant for osteoporosis. A diagnosis of primary normocalcemic hyperparathyroidism was established and the patient underwent surgery. PC was suspected intraoperatively because of the size and appearance of the parathyroid mass (a grayish, lobulated 3.5 cm mass). Thus, aggressive surgery (en bloc resection) was performed, along with bilateral neck exploration. Pathological examination of the specimens confirmed the suspicion of PC, demonstrating vascular invasion and extracapsular infiltration into adjacent soft tissue. Immunohistochemical staining revealed an elevated Ki-67 score (8.43%; cut-off value 5%). The mean area of silver-stained nucleolar orga... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5599813 Fri, 13 Jan 2012 16:58:18 +0100 5599813 http://www.medworm.com/index.php?rid=5599812&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv267560225301mm5%2F The objective of this study was to evaluate the effect of hyperlipidemia on the biomechanical and morphological properties of the femur of low-density lipoprotein receptor gene knockout mice (LDLr−/−) mice. Ten wild-type mice (C57BL6) and 10 LDLr−/− mice generated on a C57BL6 background were used. Male 3-month-old animals were divided into four groups (n = 5): group W (wild type) and group L (LDLr−/−) receiving low-fat commercial ration, and group WH (wild type) and group LH (LDLr−/−) receiving a high-fat diet. After 60 days, blood samples were collected for laboratory analysis of calcium, triglycerides, and cholesterol. The femur was excised for mechanical testing and morphometric analysis. LDLr−/− mice receiving the high-fat diet presented more mar... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5599812 Fri, 13 Jan 2012 16:58:18 +0100 5599812 http://www.medworm.com/index.php?rid=5573359&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fuv714311k0177612%2F Abstract  The aim of this study was to determine the effect of increasing estrogen and decreasing androgen in males and increasing androgen and decreasing estrogen in females on bone metabolism in patients with gender identity disorder (GID). We measured and examined bone mineral density (BMD) and bone metabolism markers retrospectively in GID patients who were treated in our hospital. In addition, we studied the effects of treatment on those who had osteoporosis. Patients who underwent a change from male to female (MtF) showed inhibition of bone resorption and increased L2–4 BMD whereas those who underwent a change from female to male (FtM) had increased bone resorption and decreased L2–4 BMD. Six months after administration of risedronate to FtM patients with osteopor... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5573359 Fri, 06 Jan 2012 06:43:44 +0100 5573359 http://www.medworm.com/index.php?rid=5573360&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F67j11644873kt84l%2F In this study, we determined the effect of homocysteine (Hcy) on the apoptosis of osteoblastic cells and investigated whether ER stress participates in Hcy-induced osteoblast apoptosis. Human osteoblastic cells were incubated with Hcy. Hcy dose-dependently decreased cell viability and increased apoptosis in osteoblastic cells. Osteoblastic cells are more susceptible to Hcy-mediated cell death than other cell types. Expression of cleaved caspase-3 was significantly increased by Hcy, and pretreatment with caspase-3 inhibitor rescued the cell viability by Hcy. Hcy treatment led to an increase in release of mitochondrial cytochrome c. It also triggered ER stress by increased expression of glucose-regulated protein 78, inositol-requiring transmembrane kinase and endonuclease 1α (IRE-1α)... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5573360 Thu, 05 Jan 2012 17:01:11 +0100 5573360 http://www.medworm.com/index.php?rid=5573361&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu8268t57vx477v00%2F This article briefly discusses the potential that some of the early consequences of phosphate toxicity might not be evident from serum phosphate levels. Content Type Journal ArticleCategory Invited ReviewPages 1-9DOI 10.1007/s00774-011-0343-zAuthors Satoko Osuka, Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Room: 304, 188 Longwood Avenue, Boston, MA 02115, USAMohammed S. Razzaque, Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Room: 304, 188 Longwood Avenue, Boston, MA 02115, USA Journal Journal of Bone and Mineral MetabolismOnline ISSN 1435-5604Print ISSN 0914-8779 (Source: Journal of Bone and Mineral Metabolism) Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5573361 Wed, 04 Jan 2012 16:51:27 +0100 5573361 http://www.medworm.com/index.php?rid=5516559&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F82w7243x6wq85r65%2F Abstract  Hypophosphatasia is a rare inherited disorder characterized by a low serum alkaline phosphatase (ALP) activity and defective bone mineralization. Adult hypophosphatasia typically manifests in middle-age as a result of osteomalacia with recurrent stress fractures of the lower limb. However, considerable variation occurs in the clinical expression of hypophosphatasia, and no curative treatment has yet been established. We herein report a case of adult hypophosphatasia with painful calcific periarthritis, which showed improvement after surgical resection. A 32-year-old male was referred to our clinic complaining of pain in his elbows and knees. A painful subcutaneous mass was palpable on his right lateral epicondyle, where periarticular calcification was detected by ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5516559 Wed, 14 Dec 2011 06:57:38 +0100 5516559 http://www.medworm.com/index.php?rid=5516560&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhj46867grt65v847%2F Abstract  Investigation of X-linked hypophosphatemia (XLH) has led to the identification of a novel phosphate-regulating homeostatic system. Initially considered vitamin D-refractory rickets, renal phosphate wasting was identified as the cardinal biochemical feature of XLH and several related disorders. Current therapy employs calcitriol and phosphate, which usually improves, but does not completely heal deformities and short stature. Later complications of XLH include development of osteophytes, entheses, and osteoarthritis. The mutated gene in XLH, PHEX, is expressed in osteocytes, but its role in the pathogenesis of phosphate wasting is poorly understood. Many hypophosphatemic disorders are mediated by FGF23, a unique fibroblast growth factor with endocrine properties. Re... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5516560 Tue, 13 Dec 2011 17:04:52 +0100 5516560 http://www.medworm.com/index.php?rid=5507206&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg1940322pl625055%2F In conclusion, anastrozole treatment for EBC in elderly women seems to have only mild negative effects on the femoral bone. Risedronate makes the use of anastrozole safer, even for osteopenic or osteoporotic elderly patients. Content Type Journal ArticleCategory Original ArticlePages 1-7DOI 10.1007/s00774-011-0341-1Authors Giuseppe Sergi, Department of Medical and Surgical Sciences, University of Padova, Padua, ItalyGiulia Pintore, Department of Medical and Surgical Sciences, University of Padova, Padua, ItalyCristina Falci, Istituto Oncologico Veneto (IOV), IRCCS, Padua, ItalyNicola Veronese, Department of Medical and Surgical Sciences, University of Padova, Padua, ItalyLinda Berton, Department of Medical and Surgical Sciences, University of Padova, Padua, ItalyEgle Perissinotto,... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5507206 Mon, 12 Dec 2011 17:14:26 +0100 5507206 http://www.medworm.com/index.php?rid=5507207&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Flm20130vj2gn1w36%2F This study investigated sexual dimorphism in hip geometry of athletes and age- and sex-matched non-athletes. Dual energy X-ray absorptiometry of the left proximal femur was performed in 62 male (n = 31; 30.2 ± 4.6 years) and female (n = 31; 27.9 ± 5.2 years) competitive endurance runners, and 36 male (n = 18; 28.7 ± 5.8 years) and female (n = 18; 29.1 ± 5.3 years) non-athletes. The hip structural analysis programme determined areal bone mineral density (aBMD), bone area (BA), hip axis length, cross-sectional area (CSA), and cross-sectional moment of inertia (CSMI) of the femoral neck. Strength indices were derived from the femoral strength index (FSI) (Yoshikawa et al., J Bone Miner Res 9:1053–1064, 1994). Despite similar... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5507207 Mon, 12 Dec 2011 06:32:59 +0100 5507207 http://www.medworm.com/index.php?rid=5478569&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy7917560x0077768%2F The objective of this study was to determine the safety and efficacy of long-term minodronate treatment in women with postmenopausal osteoporosis based on re-analysis of a phase III 2-year clinical trial with a 1-year extension. Women aged 55–80 years old with fragility fractures were enrolled and randomized to take 1 mg minodronate or placebo once a day in the original 2-year study. The subjects who completed the 2-year study were invited to participate in an additional 1-year extension in which all subjects were to receive minodronate. Finally, a total 380 subjects completed the extension study (186 from the placebo group and 194 from the minodronate group). Fracture results observed in the extension study were consistent with those observed in the first 2 years in... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5478569 Thu, 01 Dec 2011 19:27:02 +0100 5478569 http://www.medworm.com/index.php?rid=5478570&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm6q73058l5281530%2F Abstract  The intracardiac injection model is a commonly used in vivo model to test therapeutic response in bone metastases. However, few studies have critically compared the performance of different imaging methods in terms of sensitivity and quantitative assessment of osteolytic lesions. We performed in vivo optical and plain radiographic imaging of bone metastases followed by high-sensitivity ex vivo micro-computed tomography (micro-CT) imaging. This approach allowed for quantitative assessment of in vivo imaging techniques using fluorescence and plain radiography. Comparison of lesions detected in vivo by fluorescent optical imaging with ex vivo micro-CT revealed that the limited spatial resolution of fluorescent optical imaging may underestimate the number of bone meta... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5478570 Thu, 01 Dec 2011 06:59:29 +0100 5478570 http://www.medworm.com/index.php?rid=5478571&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl56415352427r75u%2F Abstract  Vitamin D insufficiency is prevalent in osteopenic and osteoporotic postmenopausal women. The persistent increase in circulating parathyroid hormone (PTH) caused by vitamin D insufficiency reduces bone density response to antiresorptive agents in these postmenopausal women. It is not well known whether administration of raloxifene might increase serum PTH secondary to the suppression of serum calcium in postmenopausal women with osteopenia or osteoporosis. We tried to assess whether raloxifene might affect serum PTH and whether the addition of alfacalcidol to raloxifene therapy could have favorable effects on bone mineral density (BMD) and bone turnover as compared to raloxifene-alone therapy in postmenopausal Japanese women with osteoporosis or osteopenia (≤2.0... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5478571 Thu, 01 Dec 2011 06:59:26 +0100 5478571 http://www.medworm.com/index.php?rid=5470545&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj146317511h244j0%2F In conclusion, at the distal femur and proximal tibia, trabecular bone contributes 66–74% of whole bone stiffness. 7T MRI and µFEA may be used as a method to provide insight into how structural properties of cortical or trabecular bone affect bone mechanical competence in vivo. Content Type Journal ArticleCategory Short CommunicationPages 1-9DOI 10.1007/s00774-011-0333-1Authors Gregory Chang, Quantitative Multinuclear Musculoskeletal Imaging Group, Center for Biomedical Imaging, Department of Radiology, NYU Langone Medical Center, 660 First Avenue, Room 231, New York, NY 10016, USAChamith S. Rajapakse, Laboratory for Structural NMR Imaging, Department of Radiology, Hospital of the University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, USAJames S. Babb, Center f... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5470545 Tue, 29 Nov 2011 22:20:10 +0100 5470545 http://www.medworm.com/index.php?rid=5450772&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fvl1131j4638l035x%2F Abstract  To better define the biologic function of membrane-bound CSF1 (mCSF1) in vivo, we have generated mCSF1 knockout (k/o) mice. Spinal bone density (BMD) was 15.9% higher in k/o mice compared to wild-type (wt) controls (P < 0.01) and total BMD was increased by 6.8% (P < 0.05). A higher mean femur BMD was also observed but did not reach statistical significance (6.9% P = NS). The osteoclastogenic potential of bone marrow isolated from mCSF1 k/o mice was reduced compared to wt marrow. There were no defects in osteoblast number or function suggesting that the basis for the high bone mass phenotype was reduced resorption. In addition to a skeletal phenotype, k/o mice had significantly elevated serum triglyceride levels (123 ± 7 ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5450772 Tue, 22 Nov 2011 06:57:50 +0100 5450772 http://www.medworm.com/index.php?rid=5450773&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ful22458wm2001167%2F In conclusion, a significant relationship exists between a change in BMD and the number of lost teeth during 5-year study period in Japanese community-dwelling postmenopausal women. Content Type Journal ArticleCategory Original ArticlePages 1-7DOI 10.1007/s00774-011-0337-xAuthors Masanori Iwasaki, Division of Preventive Dentistry, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-Dori, Chuo-Ku, Niigata, 951-8514 JapanKazutoshi Nakamura, Department of Community Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Chuo-ku, Niigata, 951-8510 JapanAkihiro Yoshihara, Division of Oral Science for Health Promotion, Department of Oral Health and Welfare, Niigata Uni... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5450773 Tue, 22 Nov 2011 06:57:49 +0100 5450773 http://www.medworm.com/index.php?rid=5450774&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb112214282385181%2F In conclusion, TPTD should be considered to avoid long-term morbidity in young patients with PLO and is highly encouraged for use in PLO patients with multiple vertebral fractures. Content Type Journal ArticleCategory Case ReportPages 1-6DOI 10.1007/s00774-011-0334-0Authors Eun Yeong Choe, Department of Internal Medicine, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 KoreaJe Eun Song, Department of Internal Medicine, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 KoreaKyeong Hye Park, Department of Internal Medicine, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 KoreaHannah Seok, Department of Internal Medicine, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemu... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5450774 Tue, 22 Nov 2011 06:57:48 +0100 5450774 http://www.medworm.com/index.php?rid=5424183&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fwx6r5347723586wu%2F This study enrolled postmenopausal women from 43 hospitals and 112 clinics who had intermittently taken oral bisphosphonates for treatment of osteoporosis. 4,220 postmenopausal Korean women with a mean age of 65.3 years and a mean body mass index of 22.9 kg/m2 participated in the study. All items within each subscale domain were more highly correlated with their hypothesized subscale domain relative to the other subscale domains, and all 16 items were significantly correlated with an overall composite satisfaction score (CSS). All scores showed acceptable internal consistency reliability (Cronbach’s alpha >0.70, range 0.88–0.91). Comparisons of OPSAT-Q scores were made between selective subgroups of participants: monthly versus weekly administration, years of taking ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5424183 Tue, 15 Nov 2011 06:49:20 +0100 5424183 http://www.medworm.com/index.php?rid=5424184&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fln1574u2330r5543%2F Abstract  Paget’s bone disease is quite common in some parts of Europe and countries inhabited by European emigrants, but it is rare in Asia. There have been only 13 reported cases in Southeast Asia, including one reported case from Thailand. Half of the previously reported cases had bone symptoms and the other half were asymptomatic, but were incidentally discovered when patients were being investigated for other medical problems. Here are reported cases of four asymptomatic patients who presented elevation of serum alkaline phosphatase during routine annual medical checkups. All patients were of Chinese descent and all cases were proven by biopsy. Based on this experience, we are of the opinion that a substantial number of unrecognized cases of Paget’s disease exist a... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5424184 Tue, 15 Nov 2011 06:49:17 +0100 5424184 http://www.medworm.com/index.php?rid=5390712&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl858783628681167%2F In conclusion, these data suggest that decreased intraosseous blood vessel volume and number may play an important role in the development of post-nerve-injury osteoporosis. Content Type Journal ArticleCategory Original ArticlePages 1-8DOI 10.1007/s00774-011-0328-yAuthors Wen-Ge Ding, Department of Orthopaedics, Third Affiliated Hospital of Suzhou University, Changzhou, 213001 ChinaWei-hong Yan, Department of Orthopaedics, Third Affiliated Hospital of Suzhou University, Changzhou, 213001 ChinaZhao-Xiang Wei, Department of Orthopaedics, Third Affiliated Hospital of Suzhou University, Changzhou, 213001 ChinaJin-Bo Liu, Department of Orthopaedics, Third Affiliated Hospital of Suzhou University, Changzhou, 213001 China Journal Journal of Bone and Mineral MetabolismOnline ISSN 14... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5390712 Mon, 07 Nov 2011 17:12:25 +0100 5390712 http://www.medworm.com/index.php?rid=5390711&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv24g1124076743qm%2F In conclusion, at the moment insufficient data are available to prove a greater risk of ONJ in children with OI than in children affected by other forms of bone fragility. However, cases may emerge in future because the risk of ONJ seems to be related to the cumulative dose and the duration of therapy. Content Type Journal ArticleCategory Original ArticlePages 1-5DOI 10.1007/s00774-011-0331-3Authors Evelina Maines, Department of Life Sciences and Reproduction Sciences, Pediatric Clinic, University of Verona, General Hospital “Giambattista Rossi”, Piazzale Ludovico Antonio Scuro 10, 37134 Verona, ItalyElena Monti, Department of Life Sciences and Reproduction Sciences, Pediatric Clinic, University of Verona, General Hospital “Giambattista Rossi”, Piazzale Ludovico Antonio Sc... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5390711 Mon, 07 Nov 2011 17:12:25 +0100 5390711 http://www.medworm.com/index.php?rid=5390713&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk5tl580m5664066v%2F We examined the differential effects of 5 E-BMP-2 treatments (0, 35, 140, 560, and 2240 μg) across 5 experimental groups (control, BMP35, BMP140, BMP560, and BMP2240). Radiography and computed tomography were used to observe the regeneration process. The groups in which higher doses of E-BMP-2 were administered (BMP560 and BMP2240) displayed more pronounced bone regeneration; the regenerated tissues connected to the host bone, and the cross-sectional areas of the regenerated bone were larger than those of the originals. The groups in which lower doses of E-BMP-2 were administered (BMP35 and BMP140) experienced relatively less bone regeneration; furthermore, the regenerated tissues failed to connect to the host bone. In these groups, the cross-sectional areas of the regenerated ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5390713 Tue, 01 Nov 2011 05:54:46 +0100 5390713 http://www.medworm.com/index.php?rid=5366712&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw177824123686t32%2F In conclusion, the SDT-fa/fa rat may be a useful model to investigate bone abnormalities in obese type 2 diabetes. Content Type Journal ArticleCategory Original ArticlePages 1-9DOI 10.1007/s00774-011-0324-2Authors Shuichi Kimura, Biological/Pharmaceutical Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1, Murasaki-cho, Takatsuki, Osaka 569-1125, JapanTomohiko Sasase, Biological/Pharmaceutical Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1, Murasaki-cho, Takatsuki, Osaka 569-1125, JapanTakeshi Ohta, Biological/Pharmaceutical Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1, Murasaki-cho, Takatsuki, Osaka 569-1125, JapanEimei Sato, Laboratory of Animal Reproducti... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5366712 Fri, 28 Oct 2011 17:17:54 +0100 5366712 http://www.medworm.com/index.php?rid=5325811&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6q732760334871k3%2F Abstract  ADAMTS1, a secreted multifunctional metalloproteinase with disintegrin and thrombospondin motifs, is an early response gene of parathyroid hormone (PTH) in osteoblasts. Mice engineered to lack Adamts1 are smaller compared to wild-type (WT) mice and ADAMTS1 metalloproteinase activity has been shown to increase osteoblastic growth in collagen gels. However, there are no reports investigating the consequence of Adamts1 over-expression on bone tissue in vivo. Here, we analyze bones of female and male transgenic (TG) mice over-expressing mouse Adamts1 using peripheral quantitative computed tomography to evaluate its effect on bone shape and mineral density. Western blotting of protein extracts and immunohistochemistry of bone sections reveal increased presence of Adamts1... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5325811 Fri, 14 Oct 2011 10:39:56 +0100 5325811 http://www.medworm.com/index.php?rid=5325812&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F571144327m016452%2F In this study osteoblast differentiation was induced in human mesenchymal stem cells (hMSCs) by treatment with bone morphogenetic protein 2 in vitro. Imatinib inhibited proliferation of hMSCs in a dose-dependent manner. Even though imatinib promoted early osteoblast differentiation assessed by alkaline phosphate activity, mineralization measured by Alizarin Red staining (ARS) was reduced by imatinib. Moreover, the inhibitory effect of imatinib on mineralization was most prominent at low concentrations of imatinib. When we measured the relative mRNA expression levels of Runx2, we found that Runx2 expression was higher in imatinib-treated (5 μM) cultures at early time points during differentiation. On the other hand, the expression of Osterix late during differentiation was lower... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5325812 Thu, 13 Oct 2011 05:51:58 +0100 5325812 http://www.medworm.com/index.php?rid=5317292&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu1m8745458723g20%2F Abstract  The present report describes a case of a 64-year-old pre-dialysis woman with chronic kidney disease (CKD) stage 5, who developed severe hyperparathyroidism. This patient had been on a very low protein diet (VLPD) to delay the progression of CKD and the need for renal replacement therapy (RRT). Her serum calcium levels were high-normal to slightly high during this time. However, her serum intact parathyroid hormone (PTH) levels increased from 400 to 1160 pg/ml rapidly over a period of 3 months. Serum 1,25-(OH)2D levels were low, and ultrasound of the neck showed three markedly enlarged parathyroid glands exceeding 2 cm. Parathyroidectomy was performed, and all glands showed nodular hyperplasia, which indicated severe secondary hyperparathyroidism lea... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5317292 Tue, 11 Oct 2011 15:53:32 +0100 5317292 http://www.medworm.com/index.php?rid=5288064&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg8473ug11m461767%2F Abstract  Maternal malnutrition during pregnancy was shown by numerous studies to result in the birth of offspring exhibiting altered bone characteristics, which are indicative of bone loss. We hypothesized that not only maternal malnutrition but also some developmental toxicants (teratogens) given at a dose inducing neither structural anomalies nor growth retardation can detrimentally affect skeletal health in adult offspring. To check this hypothesis, pregnant mice were exposed to a single injection of 5-aza-2-deoxycytidine (5-AZA) (a teratogen capable of inducing phocomelia of the hind limbs) at a sub-threshold teratogenic dose. Micro-computed tomography scanning revealed that femora of 5-month-old male offspring exposed in uterus to 5-AZA had trabecular microarchitecture... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5288064 Fri, 30 Sep 2011 05:53:51 +0100 5288064 http://www.medworm.com/index.php?rid=5288063&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq11307n3407g3184%2F Abstract  Many papers have reported that osteoclasts play an important role in the pathogenesis of rheumatoid arthritis (RA); however, when we started to investigate the pathogenesis of RA, the roles of osteoclasts were not highlighted in RA bone resorption. In recent years, the number of articles on the roles of osteoclasts and interleukin (IL)-17 in the pathogenesis of RA has increased exponentially. In this review article, we describe our articles on the roles of osteoclasts and IL-17 in joint destruction in RA, from 1990 to 2011, and highlight a novel term, ‘human osteoclastology’, which we have used since 2008. Content Type Journal ArticleCategory Review ArticlePages 1-11DOI 10.1007/s00774-011-0321-5Authors Shigeru Kotake, Institute of Rheumatology, Tokyo Wome... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5288063 Fri, 30 Sep 2011 05:53:51 +0100 5288063 http://www.medworm.com/index.php?rid=5276409&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg11p742n60826400%2F Abstract  Osteopetrosis is a heritable bone disorder resulting from a deficiency of or a functional defect in osteoclasts. We aimed to characterize the molecular defects and clinical manifestations in Chinese patients with osteopetrosis by studying 12 unrelated osteopetrosis families. The entire coding region and adjacent splice sites of the CLCN7, TCIRG1, LRP5 and SOST genes were amplified and directly sequenced. X-rays of hip and lumbar spine, bone mineral density and bone turnover markers were examined simultaneously. Family history and fracture history were collected using a questionnaire. Among 12 unrelated families, 10 families were diagnosed with autosomal dominant osteopetrosis type II (ADOII) with 10 probands and 3 affected subjects. Two individuals in the othe... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5276409 Tue, 27 Sep 2011 05:50:15 +0100 5276409 http://www.medworm.com/index.php?rid=5276411&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F07w274357pr88776%2F Abstract  Bone deformities caused by the chronic intake of large quantities of fluoride and the beneficial effect of calcium on its control have been studied for many years, but only limited data are available on the quantitative effect of fluoride intake and the beneficial impact of calcium on fluoride-induced changes in bone at the molecular level. It is necessary to determine the degree of fluoride-induced changes in bone at different levels of fluoride intake to evaluate the optimum safe intake level of fluoride for maintaining bone health and quality. The ameliorative effect of calcium at different dose levels on minimizing fluoride-induced changes in bone is important to quantify the amount of calcium intake necessary for reducing fluoride toxicity. Thirty rabbits, 2 ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5276411 Tue, 27 Sep 2011 05:50:14 +0100 5276411 http://www.medworm.com/index.php?rid=5276410&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0h64521q9544t214%2F Abstract  Receptor for hyaluronan (HA)-mediated motility (RHAMM) was first described as a soluble HA binding protein released by sub-confluent migrating cells. We previously found that RHAMM was highly expressed and plays an important role in proliferation in the human cementifying fibroma (HCF) cell line, which we previously established. HCF is a benign fibro-osseous neoplasm of the jaw and is composed of fibrous tissue containing varying amounts of mineralized material. However, the pathogenesis of HCF is not clear. In this paper, we examined the roles of RHAMM in osteoblastic cells. We generated RHAMM-overexpressing MC3T3-E1 cells and examined the cell proliferation and differentiation of osteoblastic cells. In MC3T3-E1 cells, overexpressing RHAMM was located intracellul... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5276410 Tue, 27 Sep 2011 05:50:14 +0100 5276410 http://www.medworm.com/index.php?rid=5263201&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe59370v8t6x267p3%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00774-011-0317-1Authors Hyung Jin Choi, Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, Republic of KoreaChan Soo Shin, Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, Republic of KoreaYong-Chan Ha, Department of Orthopaedic Surgery, Chung-Ang University, College of Medicine, Seoul, Republic of KoreaSunmee Jang, Health Insurance Review and Assessment Service, Seoul, Republic of KoreaSuhyun Jang, Health Insurance Review and Assessment Service, Seoul, Republic of KoreaChanmi Park, Health Insurance Review and Assessment Service, Seoul, Republic of KoreaHyun-Koo Y... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5263201 Sat, 24 Sep 2011 15:45:30 +0100 5263201 http://www.medworm.com/index.php?rid=5252428&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjv8306g34804252x%2F Abstract  Mechanical loading associated with weight-bearing physical activity has been positively associated with bone mineral density in athletes participating in various sports. The aim of this study was to compare the body composition and bone mineral density of South African male cricketers to controls. Whole body (WB), femoral neck (FN), proximal femur (PF) and lumbar spine (LS) BMD, as well as whole body fat mass (WBFM) and lean mass (WBLM) were measured, using dual-energy X-ray absorptiometry (DXA), on 34 high-performance (senior provincial and national level) cricketers and 23 physically active controls between the ages of 16 and 34 years. Cricketers were significantly younger, taller, and had greater WBLM and WBBMC compared to the controls. LS, PF and FN BMD w... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5252428 Fri, 23 Sep 2011 15:51:01 +0100 5252428 http://www.medworm.com/index.php?rid=5252429&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh5158p5n7g24m0m6%2F Abstract  Bone strength depends on several material and structural properties, but findings concerning the best predictors of bone mechanical performance are conflicting. The aim of this study was to investigate how a broad set of bone properties in the proximal femur are influenced by age and hormonal status, and how these properties together determine bone strength. Twenty-five Wistar rats were ovariectomized (OVX, n = 13) or sham operated (SHAM, n = 12) at 5 months of age, and killed after 9 months. Another group of rats was killed at 5 months as baseline control (BSL, n = 7). At sacrifice, serum 17β-estradiol and bone turnover marker concentrations were determined in the serum. Both femurs were collected for assessment of tra... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5252429 Wed, 21 Sep 2011 16:21:46 +0100 5252429 http://www.medworm.com/index.php?rid=5252430&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1p512jr16j6l6840%2F This report describes analyses based on International Conference on Harmonisation (ICH) E-5 guidelines that support bridging between Japanese studies and the large Fracture Prevention Trial (FPT). We analyzed data from single teriparatide doses in healthy Japanese and Caucasian postmenopausal women (J-PK) and from studies of 6 months [Phase 2, dose ranging (J-Ph2)] and 12 months [Phase 3, efficacy and safety (J-Ph3)] of randomized, placebo-controlled, once-daily treatment in Japanese subjects with osteoporosis. In J-PK, apparent teriparatide area-under-the-curve (AUC) and peak concentration (C max) were up to 40% higher in Japanese versus Caucasian women; however, body weight-adjusted values were comparable between populations; these findings were supported by population p... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5252430 Wed, 21 Sep 2011 16:21:45 +0100 5252430 http://www.medworm.com/index.php?rid=5252432&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp445k12232760662%2F We examined the time course of bone loss as a function of body mass index (BMI) in early and late postmenopausal women. Our study population (n = 300) was a random sample of the population-based Kuopio Osteoporosis Risk Factor and Prevention (OSTPRE) Study, Finland. We excluded women without complete BMD results, premenopausal women during the second bone densitometry and women who had used hormone replacement therapy, bisphosphonates or calcitonin. BMI along with femoral neck and spinal bone mineral density (BMD) were assessed three times by dual-energy X-ray absorptiometry during a mean follow-up of 10.5 years (SD 0.5). The mean baseline age was 53.6 years (SD 2.8), time since menopause 2.9 years (SD 4.3) and BMI 27.3 kg/m2 (SD 4.4). The data was analyz... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5252432 Wed, 21 Sep 2011 16:21:44 +0100 5252432 http://www.medworm.com/index.php?rid=5252431&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F073xj58510736805%2F Abstract  In the global Fracture Prevention Trial, teriparatide reduced the risk of vertebral and non-vertebral fractures and significantly increased BMD. Recently, a 12-month, phase 3, randomized, multicenter, double-blind, placebo-controlled trial with BMD as a primary endpoint was conducted to assess the effects of teriparatide in Japanese subjects at high risk of fracture. Although BMD was significantly increased in the Japanese study, the study was not statistically powered to assess the anti-fracture efficacy with teriparatide treatment. A meta-analysis was carried out testing whether teriparatide had consistent anti-fracture efficacy in Japanese patients compared to that observed in the global fracture trial. Three studies in which fracture data were available from p... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5252431 Wed, 21 Sep 2011 16:21:44 +0100 5252431 http://www.medworm.com/index.php?rid=5240812&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm204h861u2w3r342%2F The objective of this study was to validate a method of measuring subregional vertebral aBMD in vitro using lateral-projection DXA against subregional volumetric BMD (vBMD) measured with peripheral quantitative computed tomography (pQCT). A mixed set of 49 lumbar and thoracic vertebrae from 25 donors were scanned using lateral-projection DXA and pQCT. aBMD and apparent vBMD were measured in 7 vertebral regions (1 total area and 6 subregions) from the lateral DXA scan. vBMD was calculated in anatomically equivalent regions from pQCT scan data, using a customised software program designed to increase efficiency of the analysis process. Significant differences in densitometric parameters between subregions were observed by DXA and pQCT (P < 0.01). Subregional vBMD derived f... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5240812 Mon, 12 Sep 2011 15:51:06 +0100 5240812 http://www.medworm.com/index.php?rid=5212393&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl668770775240263%2F Abstract  Alcohol abuse and smoking habits have adverse effects on bone health and are a risk factor for osteoporosis, fractures and impaired fracture repair. Osteointegration processes around implanted biomaterials involve a coordinated cascade of complex events that are very similar to those occurring during fracture repair and require a suitable microenvironment and the coordinated action of cells and signal molecules. Therefore, diseases and harmful lifestyles that impair the normal bone healing process can reduce the success of implant surgery and may negatively influence the osteointegration of prostheses and implant devices for fracture fixation such as screws, nails and plates. Understanding the effects of harmful lifestyles on bone implant osteointegration is impor... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5212393 Tue, 06 Sep 2011 15:43:33 +0100 5212393 http://www.medworm.com/index.php?rid=5212395&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk12v72723q451431%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00774-011-0316-2Authors Eman M. Alissa, Center for Excellence for Osteoporosis Research, Faculty of Medicine, King Abdul Aziz University, PO Box 12713, Jeddah, 21483 Kingdom of Saudi ArabiaSara Ghazi Qadi, Center for Excellence for Osteoporosis Research, Faculty of Medicine, King Abdul Aziz University, PO Box 12713, Jeddah, 21483 Kingdom of Saudi ArabiaNaseem Abdulmohi Alhujaili, Center for Excellence for Osteoporosis Research, Faculty of Medicine, King Abdul Aziz University, PO Box 12713, Jeddah, 21483 Kingdom of Saudi ArabiaAfaf Mohammed Alshehri, Center for Excellence for Osteoporosis Research, Faculty of Medicine, King Abdul Aziz University, PO Box 12713, Jeddah, 21483 Kingdom of Saudi ArabiaGordon A. Ferns, Institut... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5212395 Tue, 06 Sep 2011 15:43:32 +0100 5212395 http://www.medworm.com/index.php?rid=5212394&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc2286h3808542nv1%2F Abstract  Fibroblast growth factor 23 (FGF23) is a hormone regulating phosphate and vitamin D metabolism. We have previously established a sandwich enzyme-linked immunosorbent assay (ELISA) for FGF23 and reported that FGF23 values are useful for the differential diagnosis of chronic hypophosphatemia. However, this ELISA has a rather narrow assay range of 3–800 pg/ml, and it was pointed out that the assay performance is not satisfactory when automatic washing is used. Here we evaluated a new automated chemiluminescence immunoassay for FGF23. This assay uses 10 μl sera or plasma samples and requires 20 min to obtain the first result. The assay was linear up to about 15,000 pg/ml and had a detection limit of 1 pg/ml. In addition, this assay showed co... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5212394 Tue, 06 Sep 2011 15:43:32 +0100 5212394 http://www.medworm.com/index.php?rid=5198326&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff818143x62688663%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00774-011-0315-3Authors Lisa K. Micklesfield, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South AfricaShane A. Norris, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South AfricaJohn M. Pettifor, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South Africa Journal Journal of Bone and Mineral MetabolismOnline ISSN 1435-5604Print I... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5198326 Mon, 05 Sep 2011 16:14:35 +0100 5198326 http://www.medworm.com/index.php?rid=5148730&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe0347777g563m2t1%2F Abstract  A close case study series of alendronate-associated osteonecrosis of the jaws (AONJ) is presented. A consistency of case definition with a minimal reporting requirement was attempted as recommended by the task force of the American Society for Bone and Mineral Research. A hierarchy of evidence focussing on 7 categories of interest was included for each case. A further 7 categories were added by the authors and completed where possible. The patients were drawn from a discrete population of 900,000 from the south-east of Scotland. The prescribing of drugs to this population was monitored by a government agency, the Information Services Office of the National Health Service of Scotland. The daily or weekly standard doses of alendronate prescribed to this population w... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5148730 Fri, 19 Aug 2011 15:58:17 +0100 5148730 http://www.medworm.com/index.php?rid=5148731&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F766h604140w01437%2F Abstract  Desmosterolosis is an autosomal recessive disease caused by mutations in the 3β-hydroxysterol-Delta24 reductase (DHCR24) gene, with severe developmental anomalies including short limbs. We utilized DHCR24 knockout (KO) mice to study the underlying bone pathology. Because the KO mice died within a few hours after birth, we cultured metatarsal bones from newborn mice. The growth of bones from KO mice was significantly retarded after 1 week of culture. Absence of proliferating chondrocytes in the growth plate and abnormal hypertrophy of prehypertrophic chondrocytes were observed in the bones from KO mice. Hypertrophic differentiation was evidenced by higher expression of Indian hedgehog, alkaline phosphatase, and matrix metalloproteinase 13. Since elevated leve... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5148731 Tue, 16 Aug 2011 06:10:16 +0100 5148731 http://www.medworm.com/index.php?rid=5112208&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl3u04vmk443g6704%2F Abstract  The purpose of this study was to explore whether mechanical loading by exercise over a 1–year period in postmenopausal women had an effect on the receptor activator for nuclear factor kappa B ligand/osteoprotegerin (RANKL/OPG) system or the levels of the Wnt-signaling antagonist sclerostin. A total of 112 postmenopausal were randomized to either sedentary life (controls) or physical activity (training group). Ninety-two women fulfilled the study protocol. The training program consisted of three fast 30-min walks and one or two 1-h aerobic training sessions per week. The effect on the bone mineral density of the hip assessed with dual X-ray absorptiometry was positive as reported earlier. Blood samples were taken from participants at baseline and after 1 yea... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5112208 Mon, 08 Aug 2011 05:42:08 +0100 5112208 http://www.medworm.com/index.php?rid=5112209&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9422p47436374323%2F Abstract  The purpose of this study was to: (a) determine the relationship between quantitative ultrasound (QUS) results and anthropometric, dietary and body composition factors and establish reference ranges for amplitude-dependent speed of sound (Ad-SoS) in the phalanges and broadband ultrasound attenuation (BUA) in the calcaneus of children from Extremadura, Spain, and (b) to present reference curves for this population. Healthy children (n = 245), aged 4–16 years, were included (124 girls and 121 boys). Phalangeal and calcaneal QUS measurements were performed using DBM Sonic Bone Profiler and McCue CUBA Clinical ultrasound devices, respectively. Weight, height and body mass index (BMI) were evaluated by anthropometric methods. Fat percentage, fat mass, l... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5112209 Sat, 06 Aug 2011 05:45:33 +0100 5112209 http://www.medworm.com/index.php?rid=5112210&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1138n50671863386%2F Abstract  Fibroblast growth factor 23 (FGF23) is produced by bone and reduces serum phosphate by inhibiting proximal tubular phosphate reabsorption and intestinal phosphate absorption. Excess actions of FGF23 cause several kinds of hypophosphatemic rickets/osteomalacia while deficient actions of FGF23 result in hyperphosphatemic tumoral calcinosis. In addition, FGF23 has been shown to prevent the development of hyperphosphatemia during the progression of chronic kidney disease−mineral and bone disorder. Epidemiological studies have indicated that high FGF23 levels are associated with unfavorable events including higher mortality, cardiovascular events, progression of CKD and fracture; however, these associations are not observed unequivocally and it is not evident why the... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5112210 Sat, 06 Aug 2011 05:45:32 +0100 5112210 http://www.medworm.com/index.php?rid=5058594&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ftg44114g44072412%2F Abstract  The aim of this study was to compare bone mass between two groups of jockeys (flat: n = 14; national hunt: n = 16); boxers (n = 14) and age, gender and BMI matched controls (n = 14). All subjects underwent dual energy X-ray absorptiometry (DXA) scanning for assessment of bone mass, with measurements made of the total body, vertebra L2–4 and femoral neck. Body composition and the relative contribution of fat and lean mass were extrapolated from the results. Data were analysed in accordance with differences in body composition, in particular, height, lean mass, fat mass and age. Both jockey groups were shown to display lower bone mass than either the boxers or control group at a number of sites including total body bone minera... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5058594 Tue, 19 Jul 2011 23:37:59 +0100 5058594 http://www.medworm.com/index.php?rid=5058593&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb0653j60135vxq0t%2F Abstract  We investigated the prevalence and risk factors of vertebral fractures in Korea. In a community-based prospective epidemiology study, 1,155 men and 1,529 women (mean age 59 years, range 43–74) were recruited from Ansung, a rural Korean community. Prevalent vertebral fractures were identified on the lateral spinal radiographs at T11 to L4 using vertebral morphometry. Bone mineral density (BMD) was measured at the lumbar spine, femur neck and total hip. Of the 2,684 subjects, 137 (11.9%) men and 227 (14.8%) women had vertebral fractures and the standardized prevalence for vertebral fractures using the age distribution of Korean population was 8.8% in men and 12.6% in women. In univariate analysis, older age, low hip circumference, low BMD, low income and educ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5058593 Tue, 19 Jul 2011 23:37:59 +0100 5058593 http://www.medworm.com/index.php?rid=5058596&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F22317661p6v62427%2F Abstract  To assess the ability of α-zearalanol (α-ZAL) to prevent bone loss in an ovariectomized (OVX) rat model of osteoporosis, α-ZAL was administered intragastrically to rats. After 35 days, the total body bone mineral density (BMD) was assessed in all rats. All sections were processed for immunohistochemistry and hematoxylin and eosin staining. One-way ANOVA and an LSD multiple-range test were used to determine the significant differences between groups. BMD was lower in the OVX and OVX + α-ZAL high-dose (OVX + High) groups compared to the sham-operated (Sham), OVX + 17β-ethinylestradiol (OVX + E2), OVX + α-ZAL medium-dose (OVX + Medium) and OVX + α-ZAL low-dose (OVX + Low) groups... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5058596 Tue, 19 Jul 2011 23:37:58 +0100 5058596 http://www.medworm.com/index.php?rid=5058595&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa0rrq6lxj7781644%2F Abstract  Mechanical loading has stimulating effects on bone architecture, which can potentially be used as a therapy for osteoporosis. We investigated the skeletal changes in the tibia of ovariectomized rats during treatment with whole body vibration (WBV). Different low-magnitude WBV treatment protocols were tested in a pilot experiment using ovariectomized rats with loading schemes of 2 × 8 min/day, 5 days/week (n = 2 rats per protocol). Bone volume and architecture were evaluated during a 10 week follow-up using in-vivo microcomputed tomography scanning. The loading protocol in which a 45 Hz sine wave was applied at 2 Hz with an acceleration of 0.5g showed an anabolic effect on bone and was therefore further analyzed in two groups of an... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5058595 Tue, 19 Jul 2011 23:37:58 +0100 5058595 http://www.medworm.com/index.php?rid=5042946&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb742281112137rp6%2F Abstract  Biological phenomena that exhibit periodic activity are often referred as biorhythms or biological clocks. Among these, circadian rhythms, cyclic patterns reflecting a 24-h cycle, are the most obvious in many physiological activities including bone growth and metabolism. In the late 1990s, several clock genes were isolated and their primary structures and functions were identified. The feedback loop model of transcriptional factors was proposed to work as a circadian core oscillator not only in the suprachiasmatic nuclei of the anterior hypothalamus, which is recognized as the mammalian central clock, but also in various peripheral tissues including cartilage and bone. Looking back to embryonic development, the fundamental architecture of skeletal patterning is re... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5042946 Fri, 15 Jul 2011 15:58:07 +0100 5042946 http://www.medworm.com/index.php?rid=5032195&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F677711m44224286t%2F Abstract  Mechanical stimulation plays a critical role in bone development and growth. In view of recently recognized anabolic responses promoted by a joint-loading modality, we examined the effects of elbow loading on longitudinal growth of the ulna and the humerus. Using a custom-made piezoelectric loader, the left elbow of growing C57/BL/6 female mice was given daily 5-min bouts of dynamic loading for 10 days. The right forelimbs of those mice served as contralateral controls, and the limbs of non-treated mice were used as age-matched controls. The effects of elbow loading were evaluated through measurement of bone length, weight, bone mineral density (BMD), and bone mineral content (BMC), as well as mRNA expression levels of load-sensitive transcription factors suc... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5032195 Tue, 12 Jul 2011 06:12:09 +0100 5032195 http://www.medworm.com/index.php?rid=5032196&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fdp2464w56164546h%2F We describe two staining methods using calcein green and xylenol orange, first developed in ewe bone samples and validated in human trabecular bone samples. In ewe bones, we found that calcein green at 0.5 mM concentration diluted in 100% ethanol as well as xylenol orange at 5 mM were the most adequate fluorochromes both to detect microdamage and preserve the double tetracycline labeling. These results were verified in human trabecular bone (iliac crest for the tetracycline label, and vertebral bone for the double labeling). Results obtained in human bone samples were identical to those in ewes, so this combination of fluorochromes is now used in our laboratory. Content Type Journal ArticlePages 1-9DOI 10.1007/s00774-011-0291-7Authors Brigitte Burt-Pichat, INSERM, UMR... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5032196 Tue, 12 Jul 2011 06:12:08 +0100 5032196 http://www.medworm.com/index.php?rid=5012602&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fal07564656t23415%2F In this study, the expression of MEPE and FGF-23 in OOM tumors was investigated at the transcriptional and translational levels. Eleven causative OOM tumors were analyzed by quantitative real-time reverse transcription-polymerase chain reaction and immunohistochemistry for MEPE and FGF-23 expression. Hemangiopericytomas and giant cell tumors, pathological diagnoses that are common in cases of OOM, were obtained from non-osteomalacic patients and analyzed as controls. The gene expression level of FGF23 and MEPE in OOM tumors was 104- and 105-times higher, respectively, than in non-OOM tumors. Immunohistochemical staining revealed that FGF-23 protein was expressed in all OOM tumors, and MEPE was expressed in 10 out of 11 OOM tumors. Thus, MEPE expression was common in OOM tumors, similar... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5012602 Fri, 08 Jul 2011 06:34:49 +0100 5012602 http://www.medworm.com/index.php?rid=5012603&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm6r64k10273n8278%2F Abstract  In the context of physical education curricula, markers of physical fitness (e.g., aerobic capacity, muscular strength, flexibility, and body mass index or body fat) are usually evaluated in reference to health standards. Despite their possible mediating role in the relationship between weight-bearing or muscle forces and features of bone tissue, these attributes of fitness may not be the most relevant to predict skeletal health. It is therefore important to analyze the relative contribution of these factors to the variability in bone tissue of different parts of the skeleton, and to analyze it by gender, as sensitivity to mechanical loading can diverge for boys and girls. We compared the effects of habitual physical activity (PA) and lean mass, as surrogates of w... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=5012603 Wed, 06 Jul 2011 05:49:05 +0100 5012603 http://www.medworm.com/index.php?rid=4979007&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr166678084twr274%2F Abstract  Autosomal dominant hypophosphatemic rickets (ADHR; MIM 193100) is a hereditary disorder characterized by isolated renal phosphate wasting, hypophosphatemia, and inappropriately normal 1,25-dihydroxyvitamin D3 levels. Recent studies have shown that the fibroblast growth factor 23 (FGF23) gene is responsible for this disease. FGF23 protein is a phosphaturic factor that is elevated in several diseases associated with hypophosphatemia and rickets but varies with disease status in ADHR. In the present study we observed a Chinese family of Han ethnic origin diagnosed with ADHR. The proband is a 30-year-old woman with no history of rickets but with multiple tooth abscesses as a young adult. She presented with progressive painful swelling of the left ankle after a blunt tr... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4979007 Mon, 27 Jun 2011 17:51:03 +0100 4979007 http://www.medworm.com/index.php?rid=4979008&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8041817733237280%2F We report a 26-year-old woman with severe calcinosis associated with dermatomyositis. Although calcinosis of the skin or muscles is unusual in adults with dermatomyositis, this patient developed subcutaneous calcinosis with tenderness on the arms, axillary areas, shoulder areas, chest, abdomen, pelvis, and limbs. The calcinosis steadily increased and spread until joint motions were severely limited. Radiographic examination showed extensive soft-tissue calcification with a reticular pattern and severe osteoporosis. The patient was treated with oral etidronate (800 mg/day for 3 months every 6 months) to prevent calcification of the lesions. Three months later, the patient showed a dramatic improvement in symptoms with softening of the calcinosis, reduced pain, and marke... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4979008 Mon, 27 Jun 2011 17:51:01 +0100 4979008 http://www.medworm.com/index.php?rid=4947877&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5635g6m712p45537%2F Abstract  To examine the roles of FGF and ERK MAPK signaling in osteocyte differentiation and function, we performed microarray analyses using the osteocyte cell line MLO-Y4. This experiment identified a number of mineralization-related genes that were regulated by FGF2 in an ERK MAPK-dependent manner. Real-time PCR analysis indicated that FGF2 upregulates Ank, Enpp1, Mgp, Slc20a1, and Dmp1 in MLO-Y4 cells. Consistent with this observation, the selective FGF receptor inhibitor PD173074 decreased Ank, Enpp1, Slc20a1, and Dmp1 mRNA expression in mouse calvaria in organ culture. Since Dmp1 plays a central role in osteocyte differentiation and mineral homeostasis, we further analyzed FGF regulation of Dmp1. Similar to FGF2, FGF23 upregulated Dmp1 expression in MLO-Y4 cells in the ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4947877 Thu, 16 Jun 2011 05:51:46 +0100 4947877 http://www.medworm.com/index.php?rid=4947878&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu23j8h25u34p6677%2F Abstract  A number of randomized controlled trials (RCTs) examining the role of vitamin K on bone mineral density (BMD) have yielded inconsistent results. We performed a meta-analysis of these trials to assess the effect of vitamin K on BMD. We searched MEDLINE, EMBASE and CENTRAL for relevant studies of RCTs examining the role of vitamin K on BMD. Data on participants, interventions, and outcomes were extracted and the quality of all included trials assessed. Primary outcomes for analysis were absolute changes in BMD (mg/cm2) at the lumbar spine and femoral neck. Relative changes (percentage change) in BMD at the lumbar spine were also assessed. Vitamin K supplementation was shown to be efficacious in increasing BMD at the lumbar spine but not the femoral neck. The weighte... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4947878 Wed, 15 Jun 2011 06:21:48 +0100 4947878 http://www.medworm.com/index.php?rid=4940357&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd677664023103147%2F In this study, mutation analyses were performed for COL1A1, COL1A2, CRTAP, and LEPRE1 in a cohort of 58 unrelated Chinese patients with OI; the relationship between collagen type I mutations and clinical features was examined. A total of 56 heterozygous mutations were identified in COL1A1 and COL1A2, including 43 mutations in COL1A1 and 13 mutations in COL1A2. Among the 56 causative COL1A1 and COL1A2 mutations, 24 novel mutations were found, and 25 (44.6%) resulted in the substitution of a glycine within the Gly-X-Y triplet domain of the triple helix. Compared with COL1A1 haploinsufficiency (n = 23), patients with mutations affecting glycine residues had a severe skeletal phenotype. In patients 18 years of age or older, on average patients with COL1A1 haploinsufficiency w... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4940357 Mon, 13 Jun 2011 05:35:01 +0100 4940357 http://www.medworm.com/index.php?rid=4940356&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F52r126428412n740%2F This study aimed to assess vertebral fracture risk and alendronate effects on osteoporosis in vivo using CT/FEM. Vertebral strength in 123 postmenopausal women was analyzed and the discriminatory power for vertebral fracture was assessed cross-sectionally. Alendronate effects were also prospectively assessed in 33 patients with postmenopausal osteoporosis who were treated with alendronate at a dose of 5 mg/day for 18 months. CT/FEM had higher discriminatory power for vertebral fracture than areal bone mineral density (BMD) and volumetric BMD and detected alendronate effects at 3 months. Marked bone density increases were noted in juxtacortical areas compared to inner trabecular areas. CT/FEM was useful for assessing vertebral fracture risk and therapeutic effects on os... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4940356 Mon, 13 Jun 2011 05:35:01 +0100 4940356 http://www.medworm.com/index.php?rid=4940358&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F42rl074640051224%2F Abstract  Osteoporosis is the prevalent cause of fractures in an ever-aging population, with an established correlation between daily activities and way of life. We aimed to delineate differences in onset of osteoporosis, T-score progression, quality of life, and correlation to prevalence, types, and severity of fractures in age-comparable populations of rural and metropolitan habitats in this multicenter, retrospective double-blind study. We evaluated data derived from the medical files of two comparable groups of osteoporotic patients: group A (n = 530, rural area) and group B (n = 171, metropolitan area). Both groups received comparable treatment for osteoporosis. Comparison was performed on the basis of osteoporosis onset, T-score in a maximum of 8&nbs... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4940358 Mon, 13 Jun 2011 05:35:00 +0100 4940358 http://www.medworm.com/index.php?rid=4915964&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw2q23g146170x615%2F Abstract  Osteoporosis and obesity are important public health problems in an aging society. We investigated the differential impacts of fat on bone mineral density (BMD) according to gender and menopausal status. We analyzed the baseline data of an ongoing observational cohort study, including a total of 502 healthy subjects 20–88 years of age (144 men, 159 premenopausal women, 199 postmenopausal women). Body composition and fat mass were measured using computed tomography and dual energy X-ray absorptiometry (DXA). BMD was measured at lumbar spines using DXA. In men and postmenopausal women, there was no significant correlation between fat and bone parameters after adjusting for age and body weight. However, in premenopausal women, BMD had significant negative corr... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4915964 Mon, 06 Jun 2011 05:34:37 +0100 4915964 http://www.medworm.com/index.php?rid=4915965&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5318673130502336%2F This study presents the baseline data for treatment and research on osteoporosis and provides an estimate of osteoporosis-related fractures in Korea. Content Type Journal ArticlePages 1-8DOI 10.1007/s00774-011-0279-3Authors Chanmi Park, Health Insurance Review and Assessment Service, 168 Hyoryeong-ro, Seocho-gu, Seoul, 137-706 South KoreaYong-Chan Ha, Department of Orthopaedic Surgery, Chung-Ang University College of Medicine, 224-1 Heukseok-dong, Dongjak-gu, Seoul, 156-755 South KoreaSunmee Jang, Health Insurance Review and Assessment Service, 168 Hyoryeong-ro, Seocho-gu, Seoul, 137-706 South KoreaSuhyun Jang, Health Insurance Review and Assessment Service, 168 Hyoryeong-ro, Seocho-gu, Seoul, 137-706 South KoreaHyun-Koo Yoon, Department of Medicine, Cheil General Hospital and Wome... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4915965 Mon, 06 Jun 2011 05:34:36 +0100 4915965 http://www.medworm.com/index.php?rid=4897346&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp5867q86134hk0k0%2F Abstract  We previously used five freely available bioinformatics tools (Prioritizer, Geneseeker, PROSPECTR and SUSPECTS, Disease Gene Prediction, and Endeavour) to analyze the thirteen well-replicated osteoporosis susceptibility loci and identify a subset of most likely candidate osteoporosis susceptibility genes (Huang et al. in J Hum Genet 53:644–655, 2008). In the current study, we experimentally tested the association between bone mineral density (BMD) and the 9 most likely candidate genes [LAMC2(1q25-q31), MATN3(2p24-p23), ITGAV(2q31-q32), ACVR1(2q23-q24), TDGF1(3p21.31), EGF(4q25), IGF1(12q22-q23), ZIC2(13q32), BMP2(20p12)] which were pinpointed by 4 or more bioinformatics tools. Forty tag SNPs in nine candidate genes were genotyped in a southern Chinese female case�... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4897346 Fri, 03 Jun 2011 05:58:43 +0100 4897346 http://www.medworm.com/index.php?rid=4897345&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F01vq58k265642870%2F In conclusion, we demonstrated the case of a patient with lethal perinatal hypophosphatasia caused by two novel heterozygous mutations. Content Type Journal ArticlePages 1-5DOI 10.1007/s00774-011-0282-8Authors Kai-Chi Chang, Department of Pediatrics, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, 100 TaiwanPo-Han Lin, Department of Medical Genetics, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanYi-Ning Su, Department of Medical Genetics, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanSteven Shinn-Forng Peng, Department of Medical Imaging, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Tai... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4897345 Fri, 03 Jun 2011 05:58:43 +0100 4897345 http://www.medworm.com/index.php?rid=4897347&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ffn28483n12q4v77q%2F Abstract  Soy protein consumption and exercise training have been widely studied for their effects on the vasculature and bone in healthy populations, but little is known about the effectiveness of these interventions in chronic kidney disease (CKD). Cardiovascular disease and bone fracture risk are significantly elevated in CKD, and current pharmacological interventions have been unsuccessful in treating these conditions simultaneously. The purpose of this study was to compare the effects of a soy protein diet and endurance exercise training, alone or in combination, on cardiovascular and bone health in a mouse model of renal insufficiency. At 8 weeks of age, 60 female apolipoprotein E−/− mice underwent a two-step surgical procedure to induce uremia. These mice wer... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4897347 Fri, 03 Jun 2011 05:58:42 +0100 4897347 http://www.medworm.com/index.php?rid=4897348&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6h4240221m342185%2F Abstract  We evaluated the number of osteoporosis patients under treatment and secular trends in 2005–2008 in South Korea. We investigated nationwide data regarding the number of osteoporosis patients under treatment in South Korea using data from the Health Insurance Review Agency (HIRA), which includes nationwide information. Reimbursement records from the HIRA database between 1 January 2004 and 31 December 2008 were investigated. Patients aged ≥30 years old with osteoporosis were identified based on a study-defined algorithm using prescription data and diagnostic codes. During the study periods, the number of patients receiving medical treatment related to osteoporosis increased from 1,034,399 to 1,392,189 for women and from 120,496 to 171,902 for men. The calculate... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4897348 Thu, 02 Jun 2011 06:00:09 +0100 4897348 http://www.medworm.com/index.php?rid=4897349&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8x72367158776067%2F Abstract  Tumor necrosis factor (TNF)-α exerts its biological function via TNF type 1 and type 2 receptors (TNFR1 and TNFR2). We have previously reported that bone resorption induced by lipopolysaccharide (LPS) in TNFR2-deficient mice is accelerated compared to that in wild-type (WT) mice. Although these results suggested that TNFR2 might have a protective role in bone resorption, we could not exclude the possibility that TNFR2 has no role in bone resorption. To clarify the role of TNFR2, we developed a TNF-α-induced bone resorption model using cholesterol-bearing pullulan nanogel as a TNF-α carrier to minimize the influence of inflammatory cytokines other than TNF-α. Injections of human TNF-α (hTNF), an agonist of mouse TNFR1, stimulated bone resorption lacunae on the... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4897349 Tue, 31 May 2011 05:54:18 +0100 4897349 http://www.medworm.com/index.php?rid=4856843&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F825u770675265842%2F In conclusion, although all measured BMD values were significantly higher in RG, plasma adipocytokine and ghrelin concentrations were not directly related to bone mineralization in prepubertal RG in contrast to UC. Content Type Journal ArticlePages 1-8DOI 10.1007/s00774-011-0272-xAuthors Anna-Liisa Parm, Faculty of Exercise and Sport Sciences, University of Tartu, Jakobi 5, 51014 Tartu, EstoniaJaak Jürimäe, Faculty of Exercise and Sport Sciences, University of Tartu, Jakobi 5, 51014 Tartu, EstoniaMeeli Saar, Faculty of Exercise and Sport Sciences, University of Tartu, Jakobi 5, 51014 Tartu, EstoniaKristel Pärna, Faculty of Exercise and Sport Sciences, University of Tartu, Jakobi 5, 51014 Tartu, EstoniaVallo Tillmann, Department of Pediatrics, Faculty of Medicine, University of T... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4856843 Fri, 20 May 2011 06:00:10 +0100 4856843 http://www.medworm.com/index.php?rid=4849209&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2l764mwg0567671r%2F Abstract  Our objective was to examine the effect of nutritional intake and lifestyle factors on bone mass in postmenopausal Saudi women. A total of 122 apparently healthy postmenopausal Saudi women were recruited from the Center of Excellence for Osteoporosis Research in Jeddah. A questionnaire on lifestyle habits and dietary intake was administered to all participants. Anthropometric and bone mineral density (BMD) values were measured. Fasting blood samples were taken to measure concentrations of bone-related parameters and hormones. Most of the sample population was found to be vitamin D deficient with a serum vitamin D level below 50 nmol/l. Those participants with normal BMD values had significantly lower serum vitamin D levels than osteopenic individuals (P ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4849209 Wed, 18 May 2011 18:15:39 +0100 4849209 http://www.medworm.com/index.php?rid=4849208&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa7q2771725114520%2F Abstract  Since the discovery of SOX9 mutations in the severe human skeletal malformation syndrome campomelic dysplasia in 1994, Sox9 was shown to be both required and sufficient for chondrocyte specification and differentiation. At the same time, its distant relatives Sox5 and Sox6 were shown to act in redundancy with each other to robustly enhance its functions. The Sox trio is currently best known for its ability to activate the genes for cartilage-specific extracellular matrix components. Sox9 and Sox5/6 homodimerize through domains adjacent to their Sry-related high-mobility-group DNA-binding domain to increase the efficiency of their cooperative binding to chondrocyte-specific enhancers. Sox9 possesses a potent transactivation domain and thereby recruits diverse transc... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4849208 Wed, 18 May 2011 18:15:39 +0100 4849208 http://www.medworm.com/index.php?rid=4849211&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1ht20052x60747wp%2F Abstract  The mechanism underlying the development of osteopenia or osteoporosis in longstanding phenylketonuria (PKU) remains to be clarified. We investigated the details of bone metabolism in 21 female and 13 male classical PKU patients aged 20–35 years. Vitamin D (VD), parathyroid hormone (PTH), bone turnover markers, and daily nutrient intake were examined. The patients had lower daily energy and protein intake than did the age-matched controls (22 women, 14 men), but their respective fat, VD, and calcium intake did not differ. Serum 1,25-dihydroxy VD and 25-hydroxy VD levels in female and male patient groups were significantly higher and lower than those in respective control groups (females, P < 0.001; males, P < 0.05 and P <&nb... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4849211 Wed, 18 May 2011 18:15:37 +0100 4849211 http://www.medworm.com/index.php?rid=4849210&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F95k305613225n3xn%2F Abstract  Chronic hypomagnesemia is closely associated with hypocalcemia, which is caused by impaired parathyroid hormone (PTH) secretion or the refractoriness of bone and renal tubules to PTH. The dominant mechanism of acute-onset, hypomagnesemia-induced hypocalcemia is currently unclear. An 83-year-old man who had undergone chemotherapy with carboplatin for prostate cancer suffered from acute diarrhea and finger paresthesia. Laboratory data confirmed hypocalcemia as well as hypomagnesemia. Urinary calcium levels were not measured. However, the urinary fractional excretion of Mg (FEMg) was elevated. Despite elevated PTH levels, the renal tubular maximal reabsorption rate of phosphate to GFR (TmP/GFR) was elevated, and bone formation and resorption markers were suppressed. A... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4849210 Wed, 18 May 2011 18:15:37 +0100 4849210 http://www.medworm.com/index.php?rid=4834573&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3253777333711036%2F Content Type Journal ArticlePages 1-1DOI 10.1007/s00774-011-0277-5Authors Shiyun Meng, Département de chirurgie, Faculté de médecine, Université Laval, Centre de recherche de l’Hôpital Saint-François d’Assise, CHUQ, Québec, QC, CanadaZe Zhang, Département de chirurgie, Faculté de médecine, Université Laval, Centre de recherche de l’Hôpital Saint-François d’Assise, CHUQ, Québec, QC, CanadaMahmoud Rouabhia, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Pavillon de médecine dentaire, Université Laval, Québec, QC G1V 0A6, Canada Journal Journal of Bone and Mineral MetabolismOnline ISSN 1435-5604Print ISSN 0914-8779 (Source: Journal of Bone and Mineral Metabolism) Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4834573 Thu, 12 May 2011 19:28:05 +0100 4834573 http://www.medworm.com/index.php?rid=4769073&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw6741u541uv8v0m3%2F Content Type Journal ArticlePages 1-11DOI 10.1007/s00774-011-0269-5Authors Lisa K. Micklesfield, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South AfricaShane A. Norris, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South AfricaJohn M. Pettifor, Developmental Pathways for Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd, Parktown, Johannesburg, 2193 South Africa Journal Journal of Bone and Mineral MetabolismOnline ISSN 1435-5604Print ISSN 0914-8779 (... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4769073 Mon, 25 Apr 2011 05:37:31 +0100 4769073 http://www.medworm.com/index.php?rid=4745500&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhk02301765454323%2F Abstract  Osteocytes are embedded in the bone matrix, and they communicate with adjacent osteocytes, osteoblasts, and osteoclasts through the osteocyte lacunocanalicular system. Osteocytes are believed to be essential for the maintenance of bone homeostasis because they regulate mechanical sensing and mineral metabolism in mammalian bones; however, osteocyte morphology in other vertebrates has not been well documented. We conducted a comparative study on the morphology of osteocytes and the lacunocanalicular system of the following vertebrates: two teleost fishes [medaka (Oryzias latipes), and zebrafish (Danio rerio)], three amphibians [African clawed frog (Xenopus laevis), black-spotted pond frog (Rana nigromaculata), and Japanese fire-bellied newt (Cynops pyrrhogaster)], tw... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4745500 Mon, 18 Apr 2011 05:34:07 +0100 4745500 http://www.medworm.com/index.php?rid=4688633&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr02n56m653513265%2F In conclusion, this study suggests that overweight adolescent boys have greater indices of bone axial and bending strength in comparison to controls at the femoral neck, the intertrochanteric, and the femoral shaft. Content Type Journal ArticlePages 1-8DOI 10.1007/s00774-011-0267-7Authors Zaher El Hage, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Université de Balamand, Al Koura, LebanonDenis Theunynck, EA 4110, Laboratoire RELACS, Département STAPS, Université du Littoral Côte d’Opale, 190 Avenue Schumann, Dunkerque, FranceChristophe Jacob, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Université de Balamand, Al Koura, LebanonElie Moussa, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Unive... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4688633 Wed, 06 Apr 2011 06:47:09 +0100 4688633 http://www.medworm.com/index.php?rid=4688634&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1747759376149201%2F Abstract  We aimed to clarify changes in biochemical markers of bone turnover (BTMs) over 10 years, associations with changes in bone mineral density (BMD), and birth-cohort effects in a Japanese community. We randomly selected 400 individuals (age, 40–79 years; 50 of each gender and age stratum) from a list of registered residents in 1993. We measured BMD of the spine and hip, and serum concentrations of total osteocalcin (OC), beta-C-terminal cross-linking telopeptide of type I collagen (beta-CTX), and N-terminal cross-linking telopeptide of type I collagen (NTX), in 1993 and 2003. Of the 400 subjects, 322 (153 men, 169 women) completed the 10-year follow-up. Mean change rates (standard deviation) for serum total OC, beta-CTX, and NTX over 10 years were �... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4688634 Tue, 05 Apr 2011 09:46:30 +0100 4688634 http://www.medworm.com/index.php?rid=4678689&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff828236716680735%2F Abstract  The aim of the present study was to examine whether a holistic approach combining nutrition and lifestyle counselling with the consumption of milk and yoghurt enriched with calcium, vitamin D3 and phylloquinone (vitamin K1) or menaquinone (vitamin K2) would have any additional benefit on bone mineral density (BMD) indices measured at various skeletal sites using two different techniques, dual energy X-ray absorptiometry and quantitative ultrasonography (QUS). A sample of 115 postmenopausal women were randomized to three intervention groups, receiving daily via fortified milk and yoghurt and for 12 months, 800 mg calcium and 10 μg vitamin D3 (CaD group, n = 26); 800 mg calcium, 10 μg vitamin D3 and 100 μg vitamin K1 (CaDK1 g... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4678689 Thu, 31 Mar 2011 17:37:51 +0100 4678689 http://www.medworm.com/index.php?rid=4678688&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp278r55010656j45%2F Abstract  Back and knee pain is a widespread health problem and a serious threat to the quality of life (QOL) in middle-aged and older adults, as it frequently accompanies osteoporosis and osteoarthritis. In order to compare the effects of elcatonin and risedronate on such pain, 20 units of elcatonin was intramuscularly injected to 18 patients, and 5 mg of risedronate was orally administered daily to 20 others with similar backgrounds. Exercise-induced pain was analyzed by measuring the fall of skin impedance by electroalgometry (EAM), and subjective pain was recorded by a visual rating system (VRS) on a scale of 0 (no pain) to 100 (unbearable pain). In patients treated with elcatonin, the mean EAM-estimated pain was significantly reduced after 4, 5 and 6 months o... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4678688 Thu, 31 Mar 2011 17:37:51 +0100 4678688 http://www.medworm.com/index.php?rid=4647093&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh105r4t49k535364%2F Abstract  Patients with type 2 diabetes (T2DM) are known to have increased risks of femoral neck and vertebral fractures, although their bone mineral density (BMD) is normal or even slightly increased compared to non-DM controls. This observation suggests that bone fragility not reflected by BMD, possibly deterioration of bone quality, may participate in their fracture risks. Quantitative ultrasound (QUS), unlike BMD, could possibly evaluate bone quality, especially the microarchitecture, and therefore may be useful for assessing fracture risk in T2DM. To test this hypothesis, we measured calcaneal QUS as well as BMD at the lumbar spine, femoral neck, and 1/3 radius in 96 women (mean age 66.6 years old) and 99 men (64.7 years old) with T2DM, and examined their ass... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4647093 Fri, 25 Mar 2011 05:45:06 +0100 4647093 http://www.medworm.com/index.php?rid=4562793&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr2185kj852668l13%2F Abstract  Ingestion of excess vitamin A appears to correlate with an increased fracture risk, an outcome that is likely mediated by retinoic acids (RAs); these are vitamin A metabolites that have dramatic effects on skeletal development. We studied the impacts of RA and isoform-specific RA receptor (RAR) agonists (α, β, and γ) on osteoclast formation (osteoclastogenesis) in two model systems: RAW264.7 cells and murine bone marrow-derived monocytes. The pan-RAR agonists, all-trans and 9-cis RA, inhibited receptor activator of nuclear factor kappa B ligand (RANKL)-mediated osteoclast differentiation in a concentration-dependent manner. Isoform-specific RAR agonists (α, β, and γ) also inhibited osteoclastogenesis, with the RARα agonist producing the most consistent reduc... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4562793 Mon, 07 Mar 2011 17:02:04 +0100 4562793 http://www.medworm.com/index.php?rid=4562794&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9v1r50182640tvh0%2F In conclusion, the present data suggest a high prevalence of hypovitaminosis D in perinatal pregnant Japanese women throughout the year, which seems to affect bone metabolism and to be associated with threatened premature delivery. Content Type Journal ArticlePages 1-6DOI 10.1007/s00774-011-0264-xAuthors Megumi Shibata, Division of Endocrinology and Metabolism, Department of Internal Medicine, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, JapanAtsushi Suzuki, Division of Endocrinology and Metabolism, Department of Internal Medicine, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, JapanTakao Sekiya, Department of Obstetrics and Gynecology, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoak... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4562794 Mon, 07 Mar 2011 17:02:02 +0100 4562794 http://www.medworm.com/index.php?rid=4537624&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk718847117501602%2F Abstract  Hyperphosphatemic familial tumoral calcinosis (HFTC) is known to be caused by mutations in at least three genes: FGF23, GALNT3 and KL. Two families with two affected members suffering from HFTC were scrutinized for mutations in these candidate genes. We identified in both families homozygous missense mutations affecting highly conserved amino acids in GALNT3. One of the mutations is a novel mutation, whereas the second mutation was reported before in a compound heterozygous state. Our data expand the spectrum of known mutations in GALNT3 and contribute to a better understanding of the phenotypic manifestations of mutations in this gene. Content Type Journal ArticlePages 1-5DOI 10.1007/s00774-011-0260-1Authors Alan Yancovitch, Center for Translational Genetics, ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4537624 Thu, 24 Feb 2011 08:03:55 +0100 4537624 http://www.medworm.com/index.php?rid=4523226&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F718343rv5t355703%2F Abstract  Fibroblast growth factor 23 (FGF23) plays a central role in phosphate (P) homeostasis. However, the precise mechanism of how FGF23 secretion is regulated remains to be elucidated. In the present study, we examined the effect of intravenous pamidronate administration on serum levels of FGF23. Thirteen patients with osteogenesis imperfecta were treated with two cycles of 3-day pamidronate infusion. Blood samples at pre- and post-drip pamidronate infusion were evaluated for serum calcium, P, intact PTH (iPTH), 1,25(OH)2D, intact FGF23 (FGF23), type I collagen cross-linked N-telopeptides (NTx), bone-specific alkaline phosphatase (BAP), and TmP/GFR. During the two cycles, FGF23 levels decreased significantly preceding the decline in P levels. Although the change in P l... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4523226 Tue, 22 Feb 2011 17:06:06 +0100 4523226 http://www.medworm.com/index.php?rid=4523227&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn4803jk422370247%2F Abstract  The purpose of this study was to evaluate the influence of gamma-glutamyl transferase (GGCX) gene polymorphisms on the response of serum undercarboxylated osteocalcin (ucOC) and bone turnover markers 3 months after treatment with menatetrenone. One hundred and forty postmenopausal Thai women were enrolled and assigned to receive 45 mg/day treatment of menatetrenone (MK-4) concurrently with calcium 1.2 g and vitamin D 400 IU for 3 months. Demographic characteristics, GGCX genotyping, serum bone turnover markers and ucOC levels were obtained from all participants at baseline. We evaluated the reduction of ucOC at 3 months and the reduction of beta-CTx and P1NP at 1 and 3 months. The responses were compared between the different geno... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4523227 Tue, 22 Feb 2011 17:06:05 +0100 4523227 http://www.medworm.com/index.php?rid=4499073&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh7424v5611613161%2F This study evaluated the effects on bone mineral status of long-term treatment with intranasal budesonide (INB) spray, using the recommended dose, in pediatric patients with allergic rhinitis (AR). This retrospective, case–control study of 230 prepubertal children with perennial AR, who had used nasal budesonide at a mean daily dose of 100 μg (range, 89–132 μg) for at least 3 years intermittently, was conducted from May 2007 through May 2010. The bone mineral density (BMD) of the lumbar spine was measured by dual-energy X-ray absorptiometry. Levels of serum calcium, phosphorus, alkaline phosphatase (ALP), parathyroid hormone, and osteocalcin were also assessed. The results were compared to sex- and age-matched controls (n = 140), who were newly diagnosed... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4499073 Thu, 17 Feb 2011 07:04:23 +0100 4499073 http://www.medworm.com/index.php?rid=4499075&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1127p50r082x5552%2F Abstract  One of the major benefits of a conductive PPy-based substrate is that the mediated electrical stimulation (ES) can be a stimulating factor to promote tissue regeneration. We cultured osteoblast-like Saos-2 cells on a conductive substrate made of biodegradable polylactide (95 wt%) and electrically conducting polypyrrole bioactivated with heparin (PPy/HE) (5 wt%). Using multi-well electrical cell culture plates, the effect of multiple ESs on osteoblast mineralization was investigated at various culture times. As ascertained by ARS, CPC and XPS analyses, the ES was able to promote osteoblast adhesion and growth, resulting in significantly higher calcium and phosphate content in the mineral deposition of the electrically stimulated membranes. Morphology, Ca/... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4499075 Thu, 17 Feb 2011 07:04:22 +0100 4499075 http://www.medworm.com/index.php?rid=4499074&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F95878875357744r1%2F This study was designed to evaluate the risk of Al bone toxicity among immature rats maintained at simulated high altitude (SHA) by mechanical assessment of stiffness and strength, calculation of some indicators of bone material and geometrical properties, as well as blood determinations. Forty growing rats were divided into control and experimental groups whether injected with vehicle or Al, as Al(OH)3, three times a week for 3 months. Half of each group was exposed to hypobaric conditions (HX) by placing the animals in a SHA chamber. Both treatments negatively affected structural properties of bones, decreasing the maximum capacity to withstand load, the limit elastic load and the capacity of absorbing energy in elastic conditions. Al administration significantly depressed man... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4499074 Thu, 17 Feb 2011 07:04:22 +0100 4499074 http://www.medworm.com/index.php?rid=4456901&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7t85n23757242h85%2F Abstract  Advances in bone imaging techniques have provided tools for analyzing bone structure at the macro-, micro- and nano-level. Quantitative assessment of macrostructure can be achieved using dual X-ray absorptiometry (DXA) and quantitative computed tomography (QCT), particularly volumetric quantitative CT (vQCT). In vivo quantitative techniques for assessing the microstructure of trabecular bone non-invasively and non-destructively include high-resolution CT (HR-CT) and high-resolution magnetic resonance (HR-MR). Compared with MR imaging, CT-based techniques have the advantage of directly visualizing the bone in the axial skeleton, with high spatial resolution, but the disadvantage of delivering a considerable radiation dose. Micro-CT (μCT), which provides a higher r... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4456901 Mon, 07 Feb 2011 22:17:08 +0100 4456901 http://www.medworm.com/index.php?rid=4416188&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh10653lxu2265g28%2F In conclusion, genotypes xx and pp were shown to be prognostic markers of a good response to EP treatment, whereas the XXPP haplotype carriers were revealed to have the risk factors for insufficient responsiveness against EP treatment in BMD control. Content Type Journal ArticlePages 1-9DOI 10.1007/s00774-010-0247-3Authors Elżbieta Sowińska-Przepiera, Department of Endocrinology, Metabolic Diseases and Internal Diseases, Pomeranian Medical University, ul. Unii Lubelskiej 1, 71-252 Szczecin, PolandElżbieta Andrysiak-Mamos, Department of Endocrinology, Metabolic Diseases and Internal Diseases, Pomeranian Medical University, ul. Unii Lubelskiej 1, 71-252 Szczecin, PolandKornel Chełstowski, Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, S... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4416188 Thu, 27 Jan 2011 01:06:01 +0100 4416188 http://www.medworm.com/index.php?rid=4402137&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F12607403619v1841%2F Abstract  Osteonecrosis (ON) is a rare disabling complication occurring in patients with human immunodeficiency virus (HIV) infection at a higher frequency than in the general population despite effective combination antiretroviral therapy being made available, as recently documented by several retrospective studies. We designed a multicentric case–control study among HIV-infected patients cared for at institutions in the Italian CISAI group (Italian Study Group for Adverse Events in HIV Infection) to search for additional predictors of ON in this special population. All centers which observed at least one case of ON were requested to report data for central re-evaluation. Parallel HIV-positive, ON-free controls were randomly selected and matched with confirmed cases of O... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4402137 Fri, 21 Jan 2011 20:19:12 +0100 4402137 http://www.medworm.com/index.php?rid=4384075&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq3480l8036j5k1q8%2F Abstract  Voltage-dependent L-type calcium channels (VDCC) play important roles in many cellular processes. The interaction of the actin cytoskeleton with the channel in nonexcitable cells is less well understood. We performed whole-cell patch-clamp surface biotinylation and calcium imaging on different osteoblast cells to determine channel kinetics, amplitude, surface abundance, and intracellular calcium, respectively. Patch-clamp studies showed that actin polymerization by phalloidin increased the peak current density of I Ca, whereas actin depolymerization by cytochalasin D (CD) significantly decreased the current amplitude. This result is consistent with calcium imaging, which showed that CD significantly decreased Bay K8644-induced intracellular calcium increase. Surfa... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4384075 Tue, 18 Jan 2011 18:39:20 +0100 4384075 http://www.medworm.com/index.php?rid=4353636&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj254754606565022%2F In conclusion, patients undergoing OAC treatment had a loss of trabecular and cortical bone mass, possibly due to a decrease in the γ-carboxylation of osteocalcin resulting from the vitamin K antagonism of these drugs. Content Type Journal ArticlePages 1-6DOI 10.1007/s00774-010-0250-8Authors Purificacion Rey-Sanchez, Metabolic Bone Diseases Research Group, University of Extremadura, 10071 Cáceres, SpainJesus Maria Lavado-Garcia, Metabolic Bone Diseases Research Group, University of Extremadura, 10071 Cáceres, SpainMaria Luz Canal-Macias, Metabolic Bone Diseases Research Group, University of Extremadura, 10071 Cáceres, SpainMaria Trinidad Rodriguez-Dominguez, Metabolic Bone Diseases Research Group, University of Extremadura, 10071 Cáceres, SpainJose Luis Bote-Mohedano, Metabol... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4353636 Fri, 14 Jan 2011 16:35:52 +0100 4353636 http://www.medworm.com/index.php?rid=4339886&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv34kx870541l4030%2F This study examined patients’ risk profiles and adherence to treatment in relation to the effect of risedronate and raloxifene on hip fracture incidence. Administrative billing data were used to follow two cohorts of women aged 65 and older after starting therapy with either risedronate (n = 86,735) or raloxifene (n = 37,726). The fracture risk profile was described using a 6-month history period before starting therapy. Effectiveness of each therapy was evaluated by comparing the incidence of hip fractures during the first 3 months with the subsequent 12 months among women adherent (medication possession ratio >80%) compared with those non-adherent to treatment. At the start of therapy, the raloxifene cohort was younger than the risedronate cohort (... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4339886 Tue, 11 Jan 2011 17:51:25 +0100 4339886 http://www.medworm.com/index.php?rid=4328136&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F771123jkk0188455%2F In conclusion, our results indicate for the first time that the genetic polymorphisms in SOSTDC1 have an effect on attainment and maintenance of peak bone mass in Chinese women. Content Type Journal ArticlePages 1-11DOI 10.1007/s00774-010-0253-5Authors Jin-Wei He, Metabolic Bone Disease and Genetics Research Unit, Department of Osteoporosis and Bone Diseases, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai, 600 Yi-Shan Rd, Shanghai, 200233 People’s Republic of ChinaHua Yue, Metabolic Bone Disease and Genetics Research Unit, Department of Osteoporosis and Bone Diseases, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai, 600 Yi-Shan Rd, Shanghai, 200233 People’s Republic of ChinaWei-Wei Hu, Metabolic Bone Disease and Genetics ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4328136 Fri, 07 Jan 2011 14:48:41 +0100 4328136 http://www.medworm.com/index.php?rid=4328137&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6178q72038164r78%2F Abstract  Osteoporosis is a common disease among older people, especially postmenopausal women. Calcium supplementation is effective in decreasing the occurrence of osteoporosis. We tested the effect of different calcium sources (i.e., calcium carbonate chew, milk mineral chew, milk drink and placebo chew) by direct mass spectrometry (dMS) profiling and cathepsin K measurement in the serum of subjects. The dMS method is promising for plasma biomarker discovery, and cathepsin K level in the plasma is an indicator for osteoporosis. Our result shows that dMS detected characteristic ion peaks after different calcium supplement interventions; ion peak 4281.0 m/z was commonly inhibited by all three treatments. This ion peak was identified to be a fragment of follistatin-relat... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4328137 Fri, 07 Jan 2011 06:49:38 +0100 4328137 http://www.medworm.com/index.php?rid=4325312&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmu5n78v197732314%2F Abstract  Although transforming growth factor-β (TGF-β) signaling has been implicated in cartilage formation in various studies, the exact role played by TGF-β in this process remains controversial. TGF-β signals are transduced through TGF-β type II receptor (TGF-βRΙΙ) and type I receptor (ALK5). Col2a1-Cre-mediated deletion of Tgfbr2 did not cause obvious defects in long bone formation, suggesting that TGF-β signals are dispensable for normal cartilage formation in the stage of condensing mesenchymal cells and chondrocytes or that related molecules can compensate for the lack of TGF-βRΙΙ. In the present study, we established a conditional transgenic mouse in which a dominant negative form of TGF-βRII (dnTGF-βRII) is expressed in condensing mesenchymal cells an... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4325312 Thu, 06 Jan 2011 17:53:52 +0100 4325312 http://www.medworm.com/index.php?rid=4301332&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr206106342752414%2F In conclusion, this study shows that weight, lean mass and BMI are positively associated with BMAD of the weight-bearing bones (L2−L4 and FN) but not with BMAD of the WB and the 1/3 radius in adolescent boys. Content Type Journal ArticleDOI 10.1007/s00774-010-0246-4Authors Rawad El Hage, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Université de Balamand, Al Koura, LebanonElie Moussa, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Université de Balamand, Al Koura, LebanonZaher El Hage, Laboratoire de Physiologie et de Biomécanique de la Performance Motrice, Université de Balamand, Al Koura, LebanonDenis Theunynck, EA 4110, Laboratoire RELACS, Département STAPS, Université du Littoral Côte d’Opale, 190 Avenue Schuman... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4301332 Tue, 28 Dec 2010 15:04:25 +0100 4301332 http://www.medworm.com/index.php?rid=4301331&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2085m46585213j80%2F This study shows that the simulation based on BMU activities has the potential to define and predict the morphological evolution of the vertebral body. It can be concluded that the novel algorithms incorporating the coupled effects of both adaptive remodeling and microdamage remodeling could be utilized to gain greater insight into the mechanism of bone loss in the elderly population. Content Type Journal ArticleDOI 10.1007/s00774-010-0244-6Authors Chao Wang, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 ChinaChunqiu Zhang, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4301331 Tue, 28 Dec 2010 15:04:25 +0100 4301331 http://www.medworm.com/index.php?rid=4288038&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3803207453032723%2F Abstract  Fish have recently been recognized as a suitable model and a promising alternative to mammalian systems to study skeletogenesis. In this regard, several fish bone-derived cell lines have been developed and are being used to investigate mechanisms associated with insulin-like action of vanadium on extracellular matrix (ECM) mineralization. Although proliferative and mineralogenic effects of vanadate, insulin-like growth factor 1 (IGF-1), and insulin have recently been evaluated in a fish prechondrocyte cell line, no data are available in fish bone-forming cells, the osteoblasts. Using fish preosteoblast cells, we showed that IGF-1, but not insulin or vanadate, stimulated cell proliferation through the mitogen-activated protein kinase (MAPK) pathway, while both IGF-... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4288038 Wed, 22 Dec 2010 20:15:17 +0100 4288038 http://www.medworm.com/index.php?rid=4231630&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F68l277x7230l1136%2F Abstract  In this paper we examined age-related and sex-specific deterioration in bone strength of the proximal femur reflected in mechanical properties from dual energy X-ray absorptiometry (DXA)-based hip structural analysis (HSA) on a cadaveric sample from the Balkans. Cadaveric studies permit more precise measurement of HSA parameters and allow further analyses by micromorphometric methods. DXA and HSA analysis was performed on a total of 138 cadaveric proximal femora (63 female, 75 male, age range 20–101 years) from Belgrade. HSA parameters are reported for three standard regions of the proximal femur (narrow neck, intertrochanteric, and shaft). Major age-related findings include an increase in the radius of gyration (first reported in this study), a decline in ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4231630 Fri, 03 Dec 2010 06:57:56 +0100 4231630 http://www.medworm.com/index.php?rid=4229565&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk86480801271q0u1%2F Abstract  Our goal in this study was to determine to what extent the physiologic consequences of ovariectomy (OVX) in bones are exacerbated by a lack of daily activity such as walking. We forced 14-week-old female rats to be inactive for 15 weeks with a unique experimental system that prevents standing and walking while allowing other movements. Tibiae, femora, and 4th lumbar vertebrae were analyzed by peripheral quantitative computed tomography (pQCT), microfocused X-ray computed tomography (micro-CT), histology, histomorphometry, Raman spectroscopy, and the three-point bending test. Contrary to our expectation, the exacerbation was very much limited to the cancellous bone parameters. Parameters of femur and tibia cortical bone were affected by the forced inactivity bu... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4229565 Thu, 02 Dec 2010 18:09:28 +0100 4229565 http://www.medworm.com/index.php?rid=4227298&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv0188832050758mq%2F In conclusion, Japanese osteoporotic women treated by either alendronate or risedronate showed significant improvements of geometry in proximal femur within 1 year. Content Type Journal ArticleDOI 10.1007/s00774-010-0242-8Authors Junichi Takada, Department of Orthopedic Surgery, Sapporo Medical University, Sapporo, JapanGenichiro Katahira, Sapporo Kiyota Orthopaedic Hospital, Sapporo, JapanKousuke Iba, Department of Orthopedic Surgery, Sapporo Medical University, Sapporo, JapanTakashi Yoshizaki, Kitago Orthopedic Clinic, Kitago, 3jyo-8, 5-33, Shiroishi-ku, Sapporo, 003-0833 JapanToshihiko Yamashita, Department of Orthopedic Surgery, Sapporo Medical University, Sapporo, Japan Journal Journal of Bone and Mineral MetabolismOnline ISSN 1435-5604Print ISSN 0914-8779 (Source:... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4227298 Tue, 30 Nov 2010 07:01:01 +0100 4227298 http://www.medworm.com/index.php?rid=4165463&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8566365321050047%2F Abstract  The aim of the retrospective study was to assess the influence of parity on the skeletal status in 760 postmenopausal women. Women with factors affecting their bone status, or those treated for osteoporosis, were not included. Quantitative ultrasound measurements were performed at the heel (Achilles, n = 465) or at hand phalanges (DBM Sonic 1200, n = 295). Regarding hand phalanges, Z score values were significantly lower in women with three, four, and five or six deliveries in comparison to nonparous women and in women with four deliveries versus women with one or two deliveries. Calcaneus ultrasound variables did not differ in regard to the number of deliveries. Stepwise multiple regression analysis has shown that the main factors, affecting the... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4165463 Thu, 11 Nov 2010 17:48:59 +0100 4165463 http://www.medworm.com/index.php?rid=4165464&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp0523h8221882070%2F Abstract  In January 2009, a 70-year-old Japanese woman on long-term dialysis was admitted to our hospital for evaluation of a painful left leg. Maintenance dialysis was started in 1982, and parathyroidectomy was performed for secondary hyperparathyroidism in 2004. Most of her small intestine was resected because of superior mesenteric artery thrombosis in 2006, and the remaining small bowel only extended 50 cm distal to the ligament of Treitz. Parenteral nutrition was started after bowel resection. Fracture of her left leg occurred suddenly without any precipitating factors in January 2009, and iliac bone biopsy revealed severe osteomalacia with an increase of total osteoid volume (57.6%) (>15%) and no fibrous tissue (0%) (<0.5%). Although phosphorus and active ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4165464 Thu, 11 Nov 2010 06:52:19 +0100 4165464 http://www.medworm.com/index.php?rid=4165466&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff72738w45711201m%2F Abstract  It has not been clearly defined whether reduced bone mineral density (BMD) is a direct risk factor of vascular calcification. A total of 2,160 subjects who were older than 50 years and underwent routine health examination at the Seoul National University Hospital Healthcare System Gangnam Center were included in this study. Coronary artery calcium scores (CACS) were calculated to quantify the extent of coronary artery calcification (CAC) using computed tomography. Bone dual-energy X-ray absorptiometry was also performed in all the subjects. BMD was classified as normal, osteopenia, or osteoporosis according to the lowest T score in the lumbar spine, femoral neck, or total hip. The mean value of CACS was 66.1 ± 234.0, and 1,372 subjects (63.5%) show... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4165466 Thu, 11 Nov 2010 06:52:18 +0100 4165466 http://www.medworm.com/index.php?rid=4165467&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe5p0839045266769%2F This study was performed to elucidate the effect of concomitant intake of soy isoflavone and fish oil on bone mass in ovariectomized mice. Eight-week-old ddY female mice were subjected to ovariectomy (OVX) or sham surgery, and then fed an AIN-93G with safflower oil (So) as a control lipid source, isoflavone-supplemented safflower oil (So + I), fish oil instead of safflower oil (Fo) or isoflavone-supplemented fish oil (Fo + I) for 4 weeks. Femoral bone mineral density was significantly decreased by OVX; however, this decrease was inhibited by the intake of isoflavone and/or fish oil. Histomorphometric analyses showed that bone volume and trabecular thickness in the distal femoral trabecular bone were significantly lower in the So group than in the sham group, ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4165467 Thu, 11 Nov 2010 06:52:16 +0100 4165467 http://www.medworm.com/index.php?rid=4156884&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa2756881vp2684qj%2F In conclusion, these data suggest that simvastatin exert opposing modulatory effects on ERα expression on bone and uterus in ovariectomized rats, inducing ERα expression and synergy with estrogen to perform anabolic effects on the bones while decreasing E2 efficacy and uterine wet weight. This finding may be helpful to explain the mechanism of statin treatment in osteoporosis caused by estrogen deficiency. Content Type Journal ArticleDOI 10.1007/s00774-010-0231-yAuthors Xu Li, Department of Orthopedics, Peking University Third Hospital, Beijing, 100191 ChinaQuan-Sheng Song, Department of Orthopedics, Peking University Third Hospital, Beijing, 100191 ChinaJing-Ying Wang, Department of Orthopedics, Peking University Third Hospital, Beijing, 100191 ChinaHui-jie Leng, Department of... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4156884 Tue, 09 Nov 2010 12:51:12 +0100 4156884 http://www.medworm.com/index.php?rid=4156883&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj32m10035547374j%2F This study aimed to determine whether the reproducibility of bone histomorphometry and micro-CT, and agreement between the techniques, vary in bone samples with different metabolic status. Iliac crest biopsies (n = 36) were obtained from healthy subjects (n = 10) and from patients with osteoporosis (OP) (n = 15) or renal osteodystrophy (ROD) (n = 11). Micro-CT and histomorphometry analyses were repeated twice. Results were analyzed in separate groups and after pooling the data. Bone histomorphometry detected generally known differences between the diseases, whereas micro-CT did not detect differences between normal and ROD samples as effectively. Repeated measurements for BV/TV, Tb.Th, Tb.N, and Tb.Sp exhibited linear correlation coefficients (�... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4156883 Tue, 09 Nov 2010 12:51:12 +0100 4156883 http://www.medworm.com/index.php?rid=4156885&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh72118115t64t2q5%2F In conclusion, a new murine model of hypercalcemia with anorexia was established by overexpressing sRANKL. This model would be useful for studies of hypercalcemia and coupling between bone formation and resorption. Content Type Journal ArticleDOI 10.1007/s00774-010-0235-7Authors Tetsuro Enomoto, Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd, 50 Kano-cho, Nagahama, Shiga 526-0804, JapanYuriko Furuya, Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd, 50 Kano-cho, Nagahama, Shiga 526-0804, JapanYoshiya Tomimori, Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd, 50 Kano-cho, Nagahama, Shiga 526-0804, JapanKaoru Mori, Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd, 50 Kano-cho, Nagahama, Shiga 526-0804, Ja... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4156885 Tue, 09 Nov 2010 12:51:11 +0100 4156885 http://www.medworm.com/index.php?rid=4146549&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F67085260572r82q7%2F Content Type Journal ArticleDOI 10.1007/s00774-010-0238-4Authors Evanthia Diamanti-Kandarakis, Endocrine Section, First Department of Medicine, University of Athens Medical School, Mikras Asias 75, Goudi, 115-27 Athens, GreeceSarantis Livadas, Endocrine Section, First Department of Medicine, University of Athens Medical School, Mikras Asias 75, Goudi, 115-27 Athens, GreeceIlias Katsikis, Division of Endocrinology and Human Reproduction, Second Department of Obstetrics and Gynecology, Aristotle University of Thessaloniki, 119, Mitropoleos Str., 54622 Thessaloniki, GreeceChristine Piperi, Laboratory of Biological Chemistry, University of Athens Medical School, Mikras Asias 75, Goudi, 115-27 Athens, GreeceAimilia Mantziou, Endocrine Section, First Department of Medicine, University of Ath... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4146549 Sat, 06 Nov 2010 20:19:28 +0100 4146549 http://www.medworm.com/index.php?rid=4146551&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fdq7l81163j668475%2F Abstract  Osteoarthritis (OA) is one of the most prevalent skeletal diseases. Recently, we identified a novel gene on chromosome 3p24.3, named DVWA (double von Willebrand factor A domains), and its functional variants, which are associated with susceptibility to knee OA. Here we report the cloning and characterization of the DVWA gene. DVWA consisted of seven exons and had four alternative splicing variants, which encoded long (385 amino acid) and short (276 amino acid) proteins (L-DVWA and S-DVWA, respectively). S-DVWA was an N-terminal truncated form of L-DVWA and lacked a signal peptide and a part of a VWA domain. L-DVWA and S-DVWA transcripts were mainly expressed in articular cartilage. Immunoblot analysis using epitope-tagged proteins showed L-DVWA in the conditioned m... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4146551 Sat, 06 Nov 2010 20:19:27 +0100 4146551 http://www.medworm.com/index.php?rid=4146550&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F57x00867535124r1%2F Abstract  Fracture surface analysis is a powerful technique to investigate bone failure mechanisms. Previously, emu tibiae were endocortically treated with 1 M potassium hydroxide (KOH) solution for 14 days. This treatment caused in situ collagen degradation rather than removal, with no differences in geometrical parameters, but with significant changes in mechanical properties. KOH-treated tibiae showed significant decreases in failure stress and increased failure strain and toughness. The fracture surfaces of untreated and 14-day KOH-treated failed specimens were examined to further identify differences in the failure process to explain the previously observed increase in toughness. Areas of ‘tension,’ ‘compression,’ and ‘transition’ were identified ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4146550 Sat, 06 Nov 2010 20:19:27 +0100 4146550 http://www.medworm.com/index.php?rid=4136785&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx628134796628184%2F Abstract  Infantile malignant osteopetrosis (IMO) (OMIM 259700) is a lethal autosomal recessive disease. The underlying gene in most IMO patients is TCIRG1. This codes for the TCIRG1 protein involved in the cellular proton pump, which is highly expressed on surfaces of osteoclasts. We have characterized a family comprising two affected siblings born to healthy parents. The sister and her younger brother both presented classical X-ray images of IMO at 17 h and 16 weeks, respectively, after birth, and both died after the appearance of fever and flu-like symptoms months later. Radiographs revealed normal bone density in both parents. Mutation detection of the TCIRG1 gene was performed in the boy and the parents. The novel mutation c.242delC (p.Pro81ArgfsX85) and the k... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4136785 Tue, 02 Nov 2010 15:17:42 +0100 4136785 http://www.medworm.com/index.php?rid=4112853&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy570515gk318h45w%2F In this study, we hypothesize that morpho-densitometric data describing the physical status of the pedicle isthmus in osteoporotic versus control postmenopausal women may be generated using high-resolution three-dimensional images obtained from routine abdominal multidetector computed tomography (MDCT) scans. Thus, 32 osteoporotic and 38 postmenopausal control women had a routine abdominal scan using a 16-row CT scanner. Images of the pedicle isthmus of the L2–L4 vertebrae were generated at the plane that was vertical to the pedicle axis. Several indices were calculated based on the measurements of outer and inner dimension of the pedicle isthmus, pedicle isthmus area, and pedicle isthmus endosteal area. The mean Hounsfield unit number within the isthmus endosteal area (HUIEA) and ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4112853 Mon, 25 Oct 2010 16:58:00 +0100 4112853 http://www.medworm.com/index.php?rid=4112854&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F915582r31540lt28%2F This article reviews the effect of dietary protein on bone mass and bone density, and the effect on markers of bone resorption and formation and also on fracture risk, looking at both cross-sectional and longitudinal studies and examining both meat and vegetable protein including soy and milk basic protein. The results are not entirely consistent and suggest that the interaction between dietary protein and other components in a mixed diet, such as calcium intake and vegetables and fruit to neutralize acid, are important and may determine whether high-protein diets are beneficial to bone health. Overall the results suggest that dietary protein has a modest beneficial effect on bone markers and bone density. This effect has yet to be consistently linked to reduced fracture risk, proba... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4112854 Mon, 25 Oct 2010 05:30:52 +0100 4112854 http://www.medworm.com/index.php?rid=4092166&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1761q44654344022%2F In conclusion, this study suggests an influence of training practice not only on BMD values but also on bone geometry parameters. Sports with high impacts are likely to improve bone strength and bone geometry. Moreover, this study does not support the argument that female swimmers can be considered sedentary subjects regarding bone characteristics. Content Type Journal ArticleDOI 10.1007/s00774-010-0226-8Authors Beatrice Ferry, Laboratoire Interuniversitaire de Biologie des APS, EA 3533, PRES Clermont Université, Université Blaise Pascal, 24 avenue des Landais, BP 80026, 63177 Aubiere Cedex, FranceMartine Duclos, Department of Sport Medicine and Functional Explorations, CHU Clermont-Ferrand and INRA UMR 1019, CRNH-Auvergne, Clermont-Ferrand, FranceLauren Burt, Centre of Physica... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4092166 Wed, 20 Oct 2010 17:56:58 +0100 4092166 http://www.medworm.com/index.php?rid=4070227&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn144163l4450t622%2F In conclusion, attaining optimal height for age, achieving the goals of overall nutrition with adequate calcium, iron and zinc intakes as well as adequate physical activity and sunlight exposure play an important role in achieving better TB BMC and TB BA in preschool children. Content Type Journal ArticleDOI 10.1007/s00774-010-0224-xAuthors Veena H. Ekbote, Growth and Endocrine Department, Hirabai Cowasji Jehangir Medical Research Institute, Old Building Basement, Jehangir Hospital, 32 Sassoon Road, Pune, Maharashtra 411001, IndiaAnuradha V. Khadilkar, Growth and Endocrine Department, Hirabai Cowasji Jehangir Medical Research Institute, Old Building Basement, Jehangir Hospital, 32 Sassoon Road, Pune, Maharashtra 411001, IndiaShashi A. Chiplonkar, Growth and Endocrine Department, H... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4070227 Tue, 12 Oct 2010 16:49:47 +0100 4070227 http://www.medworm.com/index.php?rid=4038557&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F66458q354804187l%2F Abstract  The aim of this study was to assess the efficacy and safety of a once-yearly zoledronic acid treatment for Chinese women with postmenopausal osteoporosis in Taiwan and Hong Kong. This post hoc subpopulation analysis, from the Health Outcome and Reduced Incidence with Zoledronic Acid One Yearly Pivotal Fracture Trial, enrolled 323 Chinese women with osteoporosis who were randomly given either annual infusions of zoledronic acid or placebo for 3 consecutive years. The incidence of fractures and changes in bone mineral density (BMD) were measured; adverse events (AEs) and tolerability were recorded and assessed. The results of this study at 36 months demonstrate that there was a significantly reduced risk of morphometric vertebral fracture and clinical vertebral... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4038557 Mon, 04 Oct 2010 12:30:04 +0100 4038557 http://www.medworm.com/index.php?rid=4033898&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F52770475x15j4k0v%2F Abstract  Runx2 plays important roles in the regulation of chondrocyte differentiation and proliferation; however, the Runx2 target molecules still remain to be investigated. We searched the genes upregulated by the introduction of Runx2 into Runx2 −/− chondrocytes using microarray and found that Tcf7 is upregulated by Runx2. Thus, we examined the functions of Runx2 in the regulation of the Tcf/Lef family of transcription factors. Runx2 induced Tcf7 and Lef1 strongly, but Tcf7l1 and Tcf7l2 only slightly in Runx2 −/− chondrocytes; the expressions of Tcf7 and Tcf7l2 were reduced in Runx2 −/− cartilaginous skeletons and calvaria, and Tcf7 showed a similar expression pattern to Runx2. In reporter assays, Runx2 mildly activated the 8.6 and 1.8 kb Tcf7 promoter c... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=4033898 Sat, 02 Oct 2010 06:02:08 +0100 4033898 http://www.medworm.com/index.php?rid=3978854&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv060852453324485%2F In conclusion no association between DXA and QUS measurements was documented in our study, whereas QUS was better correlated to biochemical indices of ROD. Content Type Journal ArticleDOI 10.1007/s00774-010-0220-1Authors Athanasios Christoforidis, 1st Paediatric Department, Aristotle University, 49, Konstantinoupoleos str., 54642 Thessaloniki, GreeceNikoleta Printza, 1st Paediatric Department, Aristotle University, 49, Konstantinoupoleos str., 54642 Thessaloniki, GreeceChrysa Gkogka, 1st Paediatric Department, Aristotle University, 49, Konstantinoupoleos str., 54642 Thessaloniki, GreeceEkaterini Siomou, Department of Child Health, Medical School, University of Ioannina, Ioannina, GreeceAnna Challa, Department of Child Health, Medical School, University of Ioannina, Ioannina, Greece... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3978854 Wed, 15 Sep 2010 16:56:09 +0100 3978854 http://www.medworm.com/index.php?rid=3967323&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fuv142g734334t030%2F This study suggests that non-cholestatic liver disease does not increase the risk of low-energy fractures during growth. From the practical perspective, however, children with chronic liver disease should be screened for history and clinical risk factors for fractures rather than referred to bone density testing. Content Type Journal ArticleDOI 10.1007/s00774-010-0219-7Authors Jerzy Konstantynowicz, Department of Pediatrics and Developmental Disorders, Bone Densitometry Unit, ‘Dr. Ludwik Zamenhof’ University Children’s Hospital, Medical University of Bialystok, Waszyngtona St 17, 15274 Bialystok, PolandDariusz M. Lebensztejn, Department of Pediatric Gastroenetrology and Allergology, Medical University of Bialystok, Bialystok, PolandElzbieta Skiba, Department of Pediatric On... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3967323 Mon, 13 Sep 2010 17:01:43 +0100 3967323 http://www.medworm.com/index.php?rid=3967322&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc71231m82826329w%2F Abstract  Interferon-inducible transmembrane protein 5 (IFITM5) is an osteoblast-specific membrane protein whose expression peaks around the early mineralization stage during the osteoblast maturation process. To investigate IFITM5 function, we first sought to identify which proteins interact with IFITM5. Liquid chromatography mass spectrometry revealed that FK506-binding protein 11 (FKBP11) co-immunoprecipitated with IFITM5. FKBP11 is the only protein it was found to interact with in osteoblasts, while IFITM5 interacts with several proteins in fibroblasts. FKBPs are involved in protein folding and immunosuppressant binding, but we could not be sure that IFITM5 participated in these activities when bound to FKBP11. Thus, we generated Ifitm5-deficient mice and analyzed their ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3967322 Mon, 13 Sep 2010 17:01:43 +0100 3967322 http://www.medworm.com/index.php?rid=3934186&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe18k3873066073x7%2F This study investigated the current incidence of hip fractures in Okinawa prefecture and compared the data with those obtained in our previous study, which was conducted using similar methods in 1987/1988. All patients, aged 50 years or older and residing in Okinawa, admitted to Okinawa hospitals in 2004 for a fresh hip fracture were identified from hospital registries. Details were obtained from the medical records and radiographs of all patients and classified according to fracture type (cervical or trochanteric), age, sex, and fracture location. Subtrochanteric fractures and pathological fractures were excluded. A total of 1,349 patients (242 men and 1,107 women) were admitted for a fresh hip fracture in 2004. Their average age was 76.9 years for men and 82.4 years ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3934186 Fri, 03 Sep 2010 15:24:28 +0100 3934186 http://www.medworm.com/index.php?rid=3931264&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq3671654j0643838%2F In this study we investigated the influence of donor age on proliferation and osteogenic differentiation in long-term ex vivo cultures of primary human MSCs from patients in different age groups. Fifteen patients (8 men/7 women) comprised three age groups: (I) <50 years, (II) 50–65 years, and (III) >65 years. MSCs harvested from bone marrow derived from routine surgical procedures were isolated and cultured in standard medium over eight passages. Osteogenic differentiation was induced by dexamethasone (10 nM), ascorbic acid (300 μM), and β-glycerophosphate (3.5 mM). Osteogenic differentiation capacity of MSCs was quantified by alkaline phosphatase (ALP) activity, fluorescence-activated cell sorting (FACS) analysis of the surface markers CD9, CD... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3931264 Thu, 02 Sep 2010 06:33:37 +0100 3931264 http://www.medworm.com/index.php?rid=3874561&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fht88l2p87341w347%2F This study is the first that has examined whether musculoskeletal degenerative diseases affect life prognosis in Japan. As many as 944 persons who were 60 years of age and older and who underwent one or more musculoskeletal checkups (knee, lower back, and bone mineral density examination) were enrolled. Survival and death after 10 years were examined. For each knee, lower back, and bone mineral density examination, subjects were divided into normal and abnormal groups. For each of the examinations (knee, lower back, or bone mineral density), 10-year mortality was compared between the two groups. Also, causes of death were examined after 10 years. As many as 805 subjects survived and 125 died. For those with and without osteoarthritis of the knee, mortality after 10&nbs... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3874561 Mon, 16 Aug 2010 05:49:34 +0100 3874561 http://www.medworm.com/index.php?rid=3866287&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fql282971q07707w0%2F This study was conducted as the pediatric part of a larger cohort study, the Japanese Population-based Osteoporosis (JPOS) study. Of 448 children aged 9–12 years who completed the baseline survey, 225 participated in the follow-up study 6 years later (follow-up rate: 50.2%). aBMD at the forearm was measured using dual-energy X-ray absorptiometry. aBMD values in pre-pubertal children at baseline showed a significant tracking correlation with aBMD obtained at 6-year follow-up in both genders (boys r = 0.655, girls r = 0.759). Although boys and girls in the lowest quartile of aBMD pre-pubertally had greater annual increases in aBMD from pre-puberty to 6-year follow-up than those in other aBMD quartiles, they still showed the lowest mean aBMD at 6-year foll... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3866287 Fri, 13 Aug 2010 17:39:47 +0100 3866287 http://www.medworm.com/index.php?rid=3866288&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp8641p5642t14n00%2F Abstract  The aim of the study was to assess body composition in subjects with phenylketonuria (PKU). Forty-five patients aged 13.8 ± 5.2 years were evaluated. Among them, 15 patients had not reached sexual maturity, showing normal serum values of phenylalanine (Phe) (subgroup 1), and 30 subjects were sexually mature (Tanner 5 stage), showing either normal serum Phe (18 cases; subgroup 2a) or increased serum Phe (12 cases; subgroup 2b). DXA-assessed spine and total body (TB) measurements [bone mineral density (BMD), bone mineral content (BMC), lean body mass (LBM) and the calculated ratios BMC/LBM] as well as laboratory parameters (serum carboxyterminal telopeptide of type I collagen, bone alkaline phosphatase, osteocalcin, parathormone, calcitonin, total an... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3866288 Thu, 12 Aug 2010 18:40:55 +0100 3866288 http://www.medworm.com/index.php?rid=3866289&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp28661h712p70rk8%2F Abstract  TAK-778, a derivative of ipriflavone, has been shown to induce bone growth both in vitro and in vivo. Recently, it has been shown that TAK-778 can enhance osteoblast differentiation of human bone marrow cells via an estrogen receptor (ER)-dependent pathway. However, the mechanism by which TAK-778 exerts its effect in vivo has not been determined. Considering the evidence that TAK-778 acts via ER-mediated signaling in vitro, in the present study we tested if TAK-778 induced osteogenesis via an ER-dependent pathway using an ovariectomized (OVX) rat model. Two weeks after test animals underwent ovariectomy, TAK-778 and/or tamoxifen was administered orally over 3 months. Vehicle-treated and sham-operated rats served as controls. The bone mineral density (BMD) of ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3866289 Wed, 11 Aug 2010 06:48:29 +0100 3866289 http://www.medworm.com/index.php?rid=3866290&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F001314h464310440%2F Abstract  The multiple factors contributing to the pathogenesis of osteoporosis include genetic and environmental factors. Because decrease in bone mineral density (BMD) is the major clinical indicator and a useful quantitative trait, many association and linkage studies of BMD have been conducted. Although the series of studies showed apparently significant associations, the genes have not been found that can be utilized in clinical practice. Several genes identified in robust genome-wide association studies will be the new cutting edge in genetic studies of osteoporosis. Our recent reports of functional single nucleotide polymorphism in the tissue-nonspecific alkaline phosphatase gene and gamma-carboxylase gene are presented in this review to discuss the future prospects ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3866290 Mon, 09 Aug 2010 19:35:41 +0100 3866290 http://www.medworm.com/index.php?rid=3866291&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff7g52685u439x8rn%2F Abstract  Dietary conjugated linoleic acid (CLA) has shown wide biologically beneficial effects, such as anticancer, antiatherosclerotic, antidiabetic, immunomodulating, and antiobesity effects. However, the effects of CLA on total body ash, reflective of bone mineral content, have not been consistent. We hypothesized that the inconsistency of the CLA effect on ash may be linked to interaction between CLA and dietary calcium levels. Thus, we investigated the effects of CLA on body ash in conjunction with various calcium levels. Male ICR mice were fed three different levels of calcium (0.01, 0.5, and 1%) with or without 0.5% CLA for 4 weeks for Experiment 1 and separate CLA isomers at 0.22% level with 1% calcium in Experiment 2. CLA feeding reduced body fat regardless o... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3866291 Mon, 09 Aug 2010 19:35:40 +0100 3866291 http://www.medworm.com/index.php?rid=3860365&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc435k693n3r41448%2F In this study, we evaluated the serum levels of osteocalcin and Gla osteocalcin and their possible associations with metabolic, hormonal, and ultrasonographic components of PSOS: 97 women were studied, 50 PCOS patients and 47 controls, matched for age and body mass index (BMI). In each subject, the levels of bone metabolism markers have been evaluated, and metabolic and hormonal profiles as well as ovarian ultrasound were carried out. Osteocalcin (4.30 ± 1.74 vs. 6.20 ± 1.78 ng/ml, P < 0.0005) values were significantly lower, whereas Gla osteocalcin (37.93 ± 6.87 vs. 9.64 ± 8.21 ng/ml, P < 0.0005) and receptor activator for nuclear factor-κB ligand (0.54 ± 0.26 vs. 0.16 ± 0.15 pm... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3860365 Sat, 07 Aug 2010 07:01:59 +0100 3860365 http://www.medworm.com/index.php?rid=3829760&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv277w013x7k4g5t7%2F Abstract  The present study was designed to investigate the effects of captopril, an angiotensin-converting enzyme inhibitor (ACEI), on bone loss in aged ovariectomized (OVX) rats and its impact on the differentiation of cultured primary osteoblasts. Ten-month-old female Sprague–Dawley rats were used for the study. After 2 months post ovariectomy (OVX), the rats were treated with captopril (1 or 5 mg/kg/day, respectively) for another 2 months. At endpoint, trabecular bone of the fourth lumbar vertebrae (L4) was undecalcified and examined by bone histomorphometry; the fifth lumbar vertebrae (L5) were examined by compression test. Primary osteoblasts were isolated from the calvaria of newborn rats and treated with different concentrations of captopril in a dif... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3829760 Thu, 05 Aug 2010 13:25:51 +0100 3829760 http://www.medworm.com/index.php?rid=3829761&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F428u0891214446q3%2F Abstract  Osteoporosis represents a growing health burden, but recognition and screening rates are low. Electronic reminders for osteoporosis have been beneficial but are not based on clinical risk factors. Available risk screening tools may contain useful constructs for creating risk-based electronic medical record (EMR) reminders. Using a cohort study design among women ≥50 years with osteoporosis or osteoporosis risk, we searched the EMR for five World Health Organization (WHO) clinical risk factors including older age, lower body mass index (BMI), low bone mineral density (BMD), history of fracture since age 50, and maternal history of osteoporosis or fracture. Rates of reporting were lower than expected for BMD (6.8%), personal history of fracture (3.5%), and ma... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3829761 Thu, 05 Aug 2010 13:25:49 +0100 3829761 http://www.medworm.com/index.php?rid=3806946&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8413v55040t3wq31%2F Abstract  Sox9 belongs to the family of Sry-related high-mobility group box transcription factors controlling cell fate, cell proliferation and differentiation in various tissues, including cartilage, testis, the central nervous system, kidney, and gastrointestine. Mice conditionally lacking Sox9 have revealed essential roles for Sox9 in these tissues. To gain further insight into the role of Sox9 in different tissues and at different stages of development, we have generated a transgenic mouse line to express Sox9 in a Cre recombinase-dependent manner. The construct contained the human cytomegalovirus enhancer and chicken β-actin promoter, and a floxed monomeric red fluorescence protein 1 (mRFP1) cassette to direct ubiquitous expression of mRFP1. Cre expression removed the m... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3806946 Fri, 30 Jul 2010 16:57:49 +0100 3806946 http://www.medworm.com/index.php?rid=3785451&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F16041225k768006g%2F In conclusion, GH administration to HX rats reactivated modeling activities in modeling predominant sites and increased new bone formation. GH administration also increases remodeling activities in remodeling predominant sites, giving limited net gain in the bone mass. Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00774-010-0210-3Authors Lysette Iglesias, Winthrop University Hospital Department of Pediatric Endocrinology 120 Professional Building Suite 210 Mineola NY 11501 USAJames K. Yeh, Winthrop University Hospital Applied Bench Core Laboratory Mineola NY 11501 USAMariano Castro-Magana, Winthrop University Hospital Department of Pediatric Endocrinology 120 Professional Building Suite 210 Mineola NY 11501 USAJohn F. Aloia, Winthrop University Hospital Applied... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3785451 Fri, 23 Jul 2010 11:07:39 +0100 3785451 http://www.medworm.com/index.php?rid=3774593&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5n571416n16l76t3%2F This study showed trabecular BMD distribution at healthy thoracic and lumbar vertebrae in Japanese subjects and specific differences in age and gender. Improved knowledge about vertebral BMD may help with the diagnosis of primary osteoporosis using MDCT. Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00774-010-0204-1Authors Tatsuro Hayashi, Gifu University Department of Intelligent Image Information, Division of Regeneration and Advanced Medical Sciences, Graduate School of Medicine 1-1 Yanagido Gifu 501-1194 JapanHuayue Chen, Gifu University Department of Anatomy, Division of Disease Control, Graduate School of Medicine Gifu JapanKei Miyamoto, Gifu University Department of Reconstructive Surgery for Spine, Bone, and Joint, Graduate School of Medicine Gifu Japan... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3774593 Fri, 16 Jul 2010 07:20:45 +0100 3774593 http://www.medworm.com/index.php?rid=3735491&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff238w32204331l45%2F We present a case of insufficient bilateral femoral subtrochanteric fractures in a patient who was treated with imatinib mesylate, an anticancer drug, for 1 year after a diagnosis of chronic myelogenous leukemia (CML). A 60-year-old woman presented with bilateral thigh pain for 6 months. A plain radiograph revealed bilateral progressive insufficient fractures on the subtrochanteric areas of the femurs. MRI of the femurs revealed incomplete stress fractures and no evidence of bone metastasis on either femur. Bone densitometry showed normal T-scores around the hip joint and spine. The patient had normal serum levels of calcium, vitamin D derivatives, and thyroid hormones. Serum phosphate levels were decreased, and parathyroid hormone levels were increased. Serum osteocalcin a... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3735491 Wed, 07 Jul 2010 06:51:24 +0100 3735491 http://www.medworm.com/index.php?rid=3735492&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn82785g7727000nw%2F Abstract  Mammalian bones have three distinct origins (paraxial mesoderm, lateral plate mesoderm, and neural crest) and undergo two different modes of formation (intramembranous and endochondral). Bones derived from the paraxial mesoderm and lateral plate mesoderm mainly form through the endochondral process. During this process, hypertrophic chondrocytes play a vital role in inducing osteogenesis. So far, a number of published papers have provided evidence that chondrocyte hypertrophy and osteoblast differentiation are controlled by a variety of signaling pathways and factors; however, little is known about their hierarchy (which are upstream? which are most potent?). In this review, we discuss the signaling pathways and transcriptional factors regulating chondrocyte hyper... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3735492 Wed, 07 Jul 2010 06:51:22 +0100 3735492 http://www.medworm.com/index.php?rid=3735493&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe105781183724815%2F In this study, we aim to determine whether seven well-replicated QTLs also contribute to BMD variation in the southern Han Chinese population. Thirty-three microsatellite markers in the proximity of seven QTLs were genotyped in 1,459 subjects from 306 families ascertained through a proband with BMD Z-score equal to or less than –1.3 at either the lumbar spine or hip. Regression-based multipoint linkage analysis was performed. In the entire study population, good linkage evidence of total hip BMD to 7p14 [maximum log of odds (LOD) score (MLS) = 2.75; nominal P = 0.0002] and 1p36 (MLS = 1.6, P = 0.003) was revealed. In the subgroup analysis of 1,166 female subjects, MLS of 3.42, 2.65, 2.42, and 1.54 were obtained on 7p12 for total hip, lumbar sp... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3735493 Wed, 07 Jul 2010 06:51:21 +0100 3735493 http://www.medworm.com/index.php?rid=3728261&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhm2n4714532374h4%2F Abstract  The aim of our study was to investigate the relationship between maternal and fetal bone turnover markers and folic acid supplementation during pregnancy. In an observational study performed in Tehran University of Medical Sciences related hospitals, 113 healthy pregnant women with gestational age between 8 and 12 weeks and aged between 15 and 42 years were recruited and followed until delivery time. The participants were divided into two groups; women who took 1 mg of folic acid daily supplement from the beginning of the pregnancy until the end of the second trimester entered into group I and women who choose to continue their daily intake of folic acid until the delivery time entered into group II. The two groups were matched based on the maternal ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3728261 Sun, 04 Jul 2010 22:44:07 +0100 3728261 http://www.medworm.com/index.php?rid=3728262&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1t26565115wrl311%2F Abstract  Parathyroid hormone (PTH) is a potent anabolic agent, but the cellular mechanisms by which it increases bone mass are not fully understood. Dickkopf 1 (Dkk1) is an endogenous inhibitor of Wnt signaling and suppresses bone formation in vivo. We sought to determine if Dkk1 and anabolic PTH treatment interact in regulating bone mass. PTH treatment of primary murine osteoblasts for 24 h reduced Dkk1 expression by 90% as quantified by real-time PCR, whereas PTH treatment in vivo reduced Dkk1 expression by 30% when given as a single daily subcutaneous dose. To directly determine whether Dkk1 modulates the anabolic response of PTH in vivo, we engineered transgenic (TG) mice expressing murine Dkk1 under the control of the 2.3-kb rat collagen alpha-1 promoter. TG mice... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3728262 Sun, 04 Jul 2010 22:44:06 +0100 3728262 http://www.medworm.com/index.php?rid=3716499&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F757j5371j1368196%2F Abstract  Mechanical forces are essential to maintain skeletal integrity, and microgravity exposure leads to bone loss. The underlying molecular mechanisms leading to the changes in osteoblasts and osteoclast differentiation and function remain to be fully elucidated. Because of the infrequency of spaceflights and payload constraints, establishing in vitro and in vivo systems that mimic microgravity conditions becomes necessary. We have established a simulated microgravity (modeled microgravity, MMG) system to study the changes induced in osteoclast precursors. We observed that MMG, on its own, was unable to induce osteoclastogenesis of osteoclast precursors; however, 24 h of MMG activates osteoclastogenesis-related signaling molecules ERK, p38, PLCγ2, and NFATc1. Rec... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3716499 Tue, 29 Jun 2010 16:31:51 +0100 3716499 http://www.medworm.com/index.php?rid=3693387&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft0114368t41l5501%2F Abstract  Vitamin D insufficiency is a risk for both skeletal and nonskeletal health. However, some ambiguity remains about threshold serum 25(OH)D for vitamin D insufficiency. To determine the threshold serum 25(OH)D to maintain normal calcium availability without elevation in serum parathyroid hormone (PTH) among Japanese subjects with various calcium intakes, we conducted a multicenter prospective open-labeled study. We recruited 107 ambulatory subjects without disorders affecting vitamin D metabolism to whom oral vitamin D3 800 IU/day for 4 weeks or 1,200 IU/day for 8 weeks was given. Serum 25(OH)D, PTH, calcium, phosphate, and magnesium were measured before and after vitamin D3 supplementation. Calcium intake was assessed by questionnaires. When all ... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3693387 Mon, 21 Jun 2010 16:01:59 +0100 3693387 http://www.medworm.com/index.php?rid=3693388&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2773867658623l6j%2F Abstract  This prospective cohort study aimed to evaluate the capacity of endogenous sex steroids to predict male osteoporosis (OP) among community-dwelling inhabitants. Among 1,028 male residents aged 40–79 years, 50 men belonging to each age stratum (200 in total) were randomly selected from a resident registration list. In the years 1993, 1996, 2000, and 2003, bone mineral density (BMD) of the lumbar spine and proximal femur was measured by dual-energy X-ray absorptiometry. Serum total estradiol (E2) and free testosterone (FT) were measured using samples extracted in 1993. Among the 200 participants at baseline, 153 subjects completed 10-year follow-ups. Mean values of serum E2 and FT were 22.4 and 9.4 pg/ml, respectively. Rates of change for BMD at the lumbar... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3693388 Mon, 21 Jun 2010 16:01:58 +0100 3693388 http://www.medworm.com/index.php?rid=3654438&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6403q1014w461573%2F We describe hereby, for the first time in the literature, the case of a patient with JPD who developed severe hypocalcemia and secondary hyperparathyroidism following effective treatment with ZOL. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s00774-010-0198-8Authors Stergios A. Polyzos, Ippokration General Hospital Department of Endocrinology 13 Simou Lianidi 551 34 Thessaloniki GreeceAthanasios D. Anastasilakis, 424 General Military Hospital Department of Endocrinology Thessaloniki GreeceIoannis Litsas, Ippokration General Hospital Department of Endocrinology 13 Simou Lianidi 551 34 Thessaloniki GreeceZoe Efstathiadou, Ippokration General Hospital Department of Endocrinology 13 Simou Lianidi 551 34 Thessaloniki GreeceMarina Kita, Ippokration General Hospital Departm... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3654438 Wed, 09 Jun 2010 09:17:23 +0100 3654438 http://www.medworm.com/index.php?rid=3654437&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe0n4g8u2514121k5%2F Abstract  The purpose of this practice-based observational study was to clarify the acute effect of risedronate on proximal femur bone mineral density (BMD) and structural geometry in patients with an increased risk of fractures. One hundred sixty-four patients (7 men and 157 postmenopausal women; mean age, 69.2 years) with osteoporosis or osteopenia and clinical risk factors of fractures were analyzed. All these patients were treated with risedronate for 1 year. Urinary levels of cross-linked N-terminal telopeptide of type I collagen (NTX) were measured at baseline and 4 months after the start of treatment. BMD of the lumbar spine and proximal femur and structural geometric parameters of the proximal femur were evaluated by dual-energy X-ray absorptiometry w... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3654437 Wed, 09 Jun 2010 09:17:23 +0100 3654437 http://www.medworm.com/index.php?rid=3642888&cid=s_33342_31_f&fid=33342&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd414234551837128%2F Abstract  Vitamin D deficiency is associated with an increased risk of many diseases (skeletal and nonskeletal). Emerging data also associate high concentrations of serum parathyroid hormone (PTH) with morbidity and increased mortality in patients both with and without known chronic kidney disease (CKD). Understanding the relationship between vitamin D and PTH and the determinants of PTH is therefore important. We performed a cross-sectional study of 203 patients with varying stages of CKD randomly recruited from the Renal Unit database at our institution. Detailed case review was performed, and samples of fasting blood were taken for biochemical analyses. We measured standard biochemistry, 25-hydroxyvitamin D (25-OHD), 1,25-OHD, and three PTH measurements [1–84 PTH, tota... Journal of Bone and Mineral Metabolism journals http://www.medworm.com/rss/comments.php?id=3642888 Wed, 02 Jun 2010 16:56:06 +0100 3642888