MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Clinical Laboratory Analysis' source. Tue, 07 Feb 2012 06:39:41 +0100 http://www.medworm.com/index.php?rid=5626026&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20503 This study was undertaken to distinguish between normal and diabetic subjects by lectin–glycated hemoglobin interaction. The quantitative precipitin method was performed for the interaction between glucose‐specific lectin Concanavalin A (Con A) and the glucose‐containing RBC‐lysate for the estimation of calculated HbA1c% from a standard curve. The standard curve was prepared by plotting the optical density of the precipitin for the interaction of standard HbA1c concentration with Con A against HbA1c reference standard. The absorbance range of the precipitate was 0.14–0.20 in normal subjects and the corresponding calculated HbA1c% along with plasma glucose (mg/dl) levels was 4.1–5.8% and 82–101 mg/dl respectively. Higher absorbance values, 0.22–0.42, were obtained in diabeti... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626026 Sun, 01 Jan 2012 05:00:00 +0100 5626026 http://www.medworm.com/index.php?rid=5626025&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20502 In conclusion, the overall picture of platelet disorders is extremely variegated, leading to numerous diagnostic and therapeutic problems whose solutions require close collaboration between clinicians and laboratory specialists. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626025 Sun, 01 Jan 2012 05:00:00 +0100 5626025 http://www.medworm.com/index.php?rid=5626024&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20501 In conclusion, miRNA146a might be implicated in lupus pathogenesis. Moreover, miRNA146a expression correlates negatively to lupus activity and LN, whereas serum IFN‐α has a direct correlation to both disease activity and nephritis; hence, both miRNA146a expression and serum IFN‐α could be potentially important diagnostic biomarkers and potential novel strategies for therapeutic interventions, which may possibly be implied to enhance the sensitivity and specificity for the prediction of flares and prognosis in SLE patients. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626024 Sun, 01 Jan 2012 05:00:00 +0100 5626024 http://www.medworm.com/index.php?rid=5626023&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20500 Analyses in the clinical area need quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas they are constructed to be simple for use, specific for the target analyte, capable of continuous monitoring and giving quick results, potentially low‐costing and portable. In this article, we describe electrochemical biosensors developed for clinical diagnosis, namely for glucose, lactate, cholesterol, urea, creatinine, DNA, antigens, antibodies, and cancer markers assays. Chosen biosensors showed desirable sensitivity, selectivity, and potential for application on real samples. They are often designed to avoid interference with undesired components present in the monitored systems. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626023 Sun, 01 Jan 2012 05:00:00 +0100 5626023 http://www.medworm.com/index.php?rid=5626022&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20499 We investigated the relationship between the urokinase type plasminogen activator receptor (uPAR) in sera and tissues of patients with cervical cancer and the clinical and pathological features of the cancer. Immunohistochemistry (SABC method) was used to detect uPAR expression in cervical cancer and normal tissues; ELISA was employed to assay the uPAR levels in cervical cancer and normal tissues and the corresponding sera. The immunohistochemistry results showed that there were 37 cases of uPAR expression in 56 patients of cervical cancer with a positive expression rate of 66%, whereas there was no uPAR expression in normal cervical tissues. The uPAR levels in cancer tissue from patients with cervical cancer (70.92 ± 28.55 ng/100 mg protein) were significantly higher than those of adjace... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626022 Sun, 01 Jan 2012 05:00:00 +0100 5626022 http://www.medworm.com/index.php?rid=5626021&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20494 Our purpose is to evaluate the predictive value of the genetic polymorphisms of Excision repair cross‐complementing group 1 (ERCC1) and xeroderma pigmentosum group D/excision repair cross‐complementing group 2 (XPD/ERCC2) in patients with advanced colorectal cancer receiving oxaliplatin‐based chemotherapy, and we performed a meta‐analysis in order to obtain a more precise estimation for a more optimizing individual chemotherapy. The relevant cohort studies were identified by searching the electronic databases of MEDLINE, EMBASE, and CNKI. We used ‘‘colorectal,’’ ‘‘cancer,’’ ‘‘carcinoma,’’ ‘‘ERCC1,’’ ‘‘XPD or ERCC2,’’ ‘‘polymorphism,’’ ‘‘oxaliplatin,’’ ‘‘treatment,’’ or ‘‘chemotherapy’’ as key words. Inclusion c... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626021 Sun, 01 Jan 2012 05:00:00 +0100 5626021 http://www.medworm.com/index.php?rid=5626020&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20492 ConclusionBy extensive serum fractionation, we detected many more proteins than in previous studies without fractionation. However, discriminating proteins were still high abundant. Results indicate that either lower abundant proteins are less distinctive, or more rigorous fractionation and selective protein depletion, or a more sensitive assay, are needed to detect lower abundant discriminative proteins. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5626020 Sun, 01 Jan 2012 05:00:00 +0100 5626020 http://www.medworm.com/index.php?rid=5419549&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20498 AbstractGlutathione S‐transferase (GST) M1 null genotype has been reported playing a significant role in the diabetes mellitus (DM) susceptibility in Turkish population. We investigated whether the GSTM1, GSTA1, and GSTP1 gene polymorphisms are associated with posttransplantation diabetes mellitus (PTDM) in Taiwan. There were 283 renal transplant recipients (RTRs) enrolled. Polymerase chain reaction–restriction fragment length polymorphism was used for the measurement of GSTA1, M1, and P1 genetic polymorphisms. PTDM was diagnosed according to the American Diabetes Association guidelines. Eight‐five patients (30%) were diagnosed with PTDM. The averaged posttransplant follow‐up period was 77.9 ± 27.2 months. Duration from transplantat to diagnosis of PTDM ranged from 0.2 to 103.1 mo... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419549 Tue, 01 Nov 2011 04:00:00 +0100 5419549 http://www.medworm.com/index.php?rid=5419548&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20497 Conclusions: DHPLC could be used to test routinely for α‐globin gene mutations and deletions. Combined with MLPA, DHPLC is a low‐cost, simple to use, accurate technique with practical value. J. Clin. Lab. Anal. 25:426–431, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419548 Tue, 01 Nov 2011 04:00:00 +0100 5419548 http://www.medworm.com/index.php?rid=5419547&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20496 Conclusion: RDW values at ≤34 weeks in newborns are higher than at ≥35 weeks. This may be useful in the differential diagnosis of neonatal hematologic diseases together with other red cell parameters. J. Clin. Lab. Anal. 25:422–425, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419547 Tue, 01 Nov 2011 04:00:00 +0100 5419547 http://www.medworm.com/index.php?rid=5419546&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20495 Conclusions: Serum cytokine levels would reflect the pathological differences of the individual treatment phases and may become useful indices in monitoring the treatment response of CHB. J. Clin. Lab. Anal. 25:414–421, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419546 Tue, 01 Nov 2011 04:00:00 +0100 5419546 http://www.medworm.com/index.php?rid=5419545&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20493 Conclusion: Serum profile of adipokines is associated with different risk factors in diabetic obese patients. J. Clin. Lab. Anal. 25:409–413, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419545 Tue, 01 Nov 2011 04:00:00 +0100 5419545 http://www.medworm.com/index.php?rid=5419544&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20491 In conclusion, the HC1 mimic peptide showed high diagnostic validity for early primary HCC, and thereby could be a candidate serum biomarker for early primary HCC. J. Clin. Lab. Anal. 25:402–408, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419544 Tue, 01 Nov 2011 04:00:00 +0100 5419544 http://www.medworm.com/index.php?rid=5419543&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20490 Conclusions: Lipophilic property appears to be an important feature of the structure in evaluating the inhibitor potential. Comparison of our findings and other authors showed that the induction of PON1 gene by naringenin and its inhibitory effects on the enzyme protein are probably two different mechanisms by which the flavonoid affects PON1. The in vitro data reported in this study could be useful in the development of structure–activity relationship for PON1 inhibition. J. Clin. Lab. Anal. 25:395–401, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419543 Tue, 01 Nov 2011 04:00:00 +0100 5419543 http://www.medworm.com/index.php?rid=5419542&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20489 We report the results of some experiments. First, we demonstrate the utility of optimal primer design and concentration in PCR by constructing suboptimal primers, for instance with hairpin and primer–dimers secondarystructures, and quantifying the decrease in efficiency of amplification. Second, we show the adverse effects of the target sequence harboring stable secondary structures on the primer binding sites. Finally, we let see that the mere use of probe‐based detection is not enough to ensure robustness of qPCR data, because the eventual detrimental products generated by primers not well designed may influence in any case the PCR efficiency. J. Clin. Lab. Anal. 25:389–394, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419542 Tue, 01 Nov 2011 04:00:00 +0100 5419542 http://www.medworm.com/index.php?rid=5419541&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20479 Conclusion: Our data show a high prevalence of TST and QFT‐G positivity in laboratory personnel and emphasize the importance of LTBI screening for laboratory personnel. In BCG‐vaccinated populations with an intermediate incidence setting, QFT‐G seems to be superior to TST as a screening tool for the detection of LTBI. Further study, including results of follow‐up tests will be helpful for confirmation of our findings. J. Clin. Lab. Anal. 25:382–388, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419541 Tue, 01 Nov 2011 04:00:00 +0100 5419541 http://www.medworm.com/index.php?rid=5419540&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20455 Conclusions: We sustain that high levels of hsCRP are associated with disturbance in carbohydrate metabolism. In addition, we believe that in low cardiovascular risk population, such as NG women, uric acid levels above 3.9 mg/dl might represent a signal of possible pro‐inflammatory state and cardiovascular risk. J. Clin. Lab. Anal. 25:375–381, 2011. © 2011 Wiley Periodicals, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5419540 Tue, 01 Nov 2011 04:00:00 +0100 5419540 http://www.medworm.com/index.php?rid=5220484&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20488 AbstractHydroxyurea (HU) is the only drug approved for the induction of fetal hemoglobin. Besides this benefit, there are others such as the reduction of leukocyte and generation of nitric oxide (NO). Sickle cell anemia (SCA) is characterized by chronic hemolytic anemia and vaso‐occlusive phenomena. The aim of this study was to evaluate the correlation of parameters MDA and NO2 with the prognosis of patients with SCA as outpatients at Hospital Universitário Walter Cantídeo. In all, 65 patients with SCA—51 without the use of HU (group I) and 14 chronically treated with HU (group II)—were recruited. Nitrite and malonaldehyde were determined by biochemical methods. We found that in group II therewas a significant difference of serum MDA with clinical variables: two or more transfusion... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220484 Sat, 01 Jan 2011 05:00:00 +0100 5220484 http://www.medworm.com/index.php?rid=5220483&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20487 The objective of this study was to discover a method to stabilize the blood samples for the determination of MetHb. First, hemolysates were prepared by diluting the MetHb blood samples with phosphate buffers under different pH values. The samples were stored at 4–8°C and a day‐to‐day variability in the amount of MetHb was determined using the method described by Evelyn and Malloy. The results show that there is a significantchange in the amount of MetHbstored in both KH2PO4/Na2HPO4 and KH2PO4/Na2HPO4.2H2O buffer solutions at pH of 6.7 and 6.9. Buffer solution containing phosphate composition of KH2PO4/Na2HPO4·2H2O (pH=7.0) gives relatively stable values for MetHb during the storage and the amount of MetHb samples in the buffer solution retain constant up to 9 days. Therefore, stabi... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220483 Sat, 01 Jan 2011 05:00:00 +0100 5220483 http://www.medworm.com/index.php?rid=5220482&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20486 Conclusions: The two‐step assay might be used when the FISH signal is weak, bacterial numbers are low or if there is a need to use other reporter molecules. J. Clin. Lab. Anal. 25:359–365, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220482 Sat, 01 Jan 2011 05:00:00 +0100 5220482 http://www.medworm.com/index.php?rid=5220481&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20485 This article summarizes urinary biomarkers of smoke exposure which can be now quantified. The most reliable urinary biomarkers to assess the exposure to SHS are NNAL 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanol and NNAL‐Glucuronides, which is metabolites of tobacco‐specific nitrosamine. These substances were detected even in 50% of children who had undetectable level of cotinine (<0.5 ng/ml). Urinary cotinine, which is determined by a highly sensitive competing enzyme immunoassay, is also a useful biomarker. However, individual variability of CYP2A6 allele,in which nicotine is catalyzed to cotinine, affects the level of urinary cotinine. Approximately 20% of Japanese subjects have homozygotes or heterozygotes of the CYP2A6*4 allele, which has impaired nicotine metabol... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220481 Sat, 01 Jan 2011 05:00:00 +0100 5220481 http://www.medworm.com/index.php?rid=5220480&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20484 Abstractα‐Feto protein (AFP) is the widely used tumor marker in the diagnosis of hepatocellular carcinoma (HCC). The aim of this study was to assess the diagnostic and prognostic validity of a novel marker, serum Glypican‐3 (GPC3) and to compare AFP in patients with HCC. One hundred and twenty‐eight patients (75 patients with HCC, 55 patients with cirrhosis, and 28 healthy controls) were included in this study. Cut‐off value of GPC3 was 3.9 pg/ml. AFP was divided into four subgroups, according to cut‐off values with 13, 20, 100, and 200 ng/ml. Sensitivity, specificity, and positive and negative predictive values of GPC3 and AFP13, AFP20, AFP100, AFP200 subgroups and also GPC3+AFP13, GPC3+AFP20, GPC3+AFP100, GPC3+AFP200 combinations were compared. Serum GPC3 levels were signi... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220480 Sat, 01 Jan 2011 05:00:00 +0100 5220480 http://www.medworm.com/index.php?rid=5220479&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20483 In this study, a novel fusion protein was constructed using gene splicing by overlap extension (SOEing), and then the antibody level against it in 171 TB patients and 86 controls was evaluated by enzyme‐linked immunosorbent assay. Compared with the three individual antigen (16 kDa: sensitivity 19.9%, specificity 96.5%; MPT64: sensitivity 75.4%, specificity34.9%; 38 kDa: sensitivity 33.3%, specificity 83.7%), the fusion protein antigen (sensitivity 42.1%, specificity 89.5%) gave the best diagnostic performance with the largest receiver operating characteristic curve area 0.656 (95% confidence interval [CI], 0.590–0.721; P<0.01). These results suggested that the novel fusion protein antigen successfully constructed by gene SOEing provided the improved diagnostic performance for TB... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220479 Sat, 01 Jan 2011 05:00:00 +0100 5220479 http://www.medworm.com/index.php?rid=5220478&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20482 AbstractExternal Quality Assessment (EQA) is an essential tool for laboratories to monitor the performances of their analyses. It also allows a comparison of methods and types of laboratories (professional laboratories vs. medical offices). We, therefore, compared 55,769 HbA1c EQA results obtained between 1999 and 2008 by laboratories participating in EQA schemes organized by two European centers, Switzerland (center 1) and France (center 2). We used simple, nonparametrical statistics suited to EQA results to calculate the yearly and global precision performances. All the results, including the outliers, were included in the calculations. The best global precision performances were obtained by professional laboratories and medical offices using DCA POCT devices, followed byprofessional lab... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220478 Sat, 01 Jan 2011 05:00:00 +0100 5220478 http://www.medworm.com/index.php?rid=5220477&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20480 We describe a novel approach for distinguishing fibrinogen spikes from true monoclonal spikes. We classified 600 individual patient samples into four groups: group 1, 58 samples with a fibrinogen spike; group 2, 127 samples with a spike due to a monoclonal gammopathy; group 3, 181 samples with previously established monoclonal gammopathies but resolved posttreatment; and group 4, 234 control samples without monoclonal gammopathies. The value of using a γ regionfraction/IgG ratio in distinguishing fibrinogen from true monoclonal spikes was assessed. The γ/IgG ratio in the fibrinogen group is significantly (P<0.0001) higher than this ratio in the other three groups. A γ/IgG ratio cut‐off value of 1.13 discriminates true monoclonal gammopathies from fibrinogen. Moreover, exclusion of ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220477 Sat, 01 Jan 2011 05:00:00 +0100 5220477 http://www.medworm.com/index.php?rid=5220476&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20478 (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220476 Sat, 01 Jan 2011 05:00:00 +0100 5220476 http://www.medworm.com/index.php?rid=5220475&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20477 Conclusion: Nephelometry is the clinical urinary albumin method with best analytical performance in our study. J. Clin. Lab. Anal. 25:324–329, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220475 Sat, 01 Jan 2011 05:00:00 +0100 5220475 http://www.medworm.com/index.php?rid=5220474&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20476 In this study, we estimated the reference intervals of the serum homocysteine (Hcy) level using two automated immunoassays, and we demonstrated the effects of various factors on the Hcy level in a Korean population. We calculated the gender‐ and assay‐specific reference intervals using the data from 809 healthy Koreans, and we assessed the effects of physiologic and lifestyle factors on the Hcy level. The upper limit was higher in males (19.21 and 19.76 μmol/l) than that in females (14.99 μmol/l and 15.16 μmol/l, AxSym and ADVIA centaur, respectively); the upper limits were comparable between the two assays. Smokers, vitamin nonusers, and persons without regular exercise showed a lower folate level and a higher Hcy level. The risk of hyperhomocysteinemia was significantly asso... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220474 Sat, 01 Jan 2011 05:00:00 +0100 5220474 http://www.medworm.com/index.php?rid=5220473&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20473 AbstractThe aim of this study was to determine and evaluate the activity of paraoxonase and arylesterase enzymes in various clinical forms of hepatitis B infection and to investigate the correlation between these parameters and chronic disease course/fibrosis. Overall, 40 patients diagnosed as hepatitis B carriers (CIHBV), 40 chronic active hepatitis B (CAHBV) patients, and 40 healthy adults (control group) between 18 and 65 years of age were enrolled the study. Serum paraoxonase and arylesterase activities were measured spectrophotometrically. Their activities were significantly lower in patients with CAHBV compared with CIHBV patients or with control group patients (P<0.001). There was a negative correlation between alanine aminotransferase levels and theactivity of paraoxonase and ar... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5220473 Sat, 01 Jan 2011 05:00:00 +0100 5220473 http://www.medworm.com/index.php?rid=5057621&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20481 (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057621 Sat, 01 Jan 2011 00:00:00 +0100 5057621 http://www.medworm.com/index.php?rid=5057620&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20475 AbstractCorrelation of serologic titers for Chlamydia trachomatis with other tests has been based on direct fluorescence antibody (DFA) testing and culture, but not on nucleic acid‐based tests that are used for screening. We retrospectively reviewed the specificity of antibodies against C. trachomatis, Chlamydia psittaci, and Chlamydophila pneumoniae by microimmunofluorescence (MIF) when compared with DFA, culture, nucleic acid probe, and transcription‐mediated amplification. Over a 6‐year period, 226 cases had both MIF and one of these other methods performed for comparison. Agreement between C. trachomatis antigen or nucleic acid detection and MIF results was 87% (197/226). C. trachomatis serology had a negative predictive value of 98%, and 10.6% of cases were positive by serology ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057620 Sat, 01 Jan 2011 00:00:00 +0100 5057620 http://www.medworm.com/index.php?rid=5057619&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20474 Conclusions: The HRM method with unlabeled probe could be used as convenient, sensitive and reliable diagnostic test for detection of JAK2 V617F mutation. J. Clin. Lab. Anal. 25:300–304, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057619 Sat, 01 Jan 2011 00:00:00 +0100 5057619 http://www.medworm.com/index.php?rid=5057618&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20472 AbstractBackground: Alpha 1 antitrypsin (AT) deficiency is a hereditary disorder leading to the defective defence system against neutrophil elastasis in lung and accumulation of insoluble heterodimer AT molecules in hepatocytes. Knowledge of the prevalence of AT deficiency in each country is important to organize the public health policy. The aim of this study is to determine the prevalence of AT deficiency in Turkish population and to define the cutoff value of AT level in serum to detect heterozygous AT deficient subjects. Materials and Methods: Serum samples from 1,203 healthy blood donors were used, attending the Blood Bank of Hacettepe Medical Faculty. Isoelectric focusing method for determining PIM, PIS, and PIZ alleles and rate immune nephelometry for measuring the level of AT in se... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057618 Sat, 01 Jan 2011 00:00:00 +0100 5057618 http://www.medworm.com/index.php?rid=5057617&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20471 Conclusions: These results suggest that according to the criteria of the IDF, the significant decrease observed in serum paraoxonase activity was independent of the metabolic syndrome in patients with mild‐to‐moderate plaque‐type psoriasis, whereas the significant decrease in serum arylesterase activity was associated with the metabolic syndrome. J. Clin. Lab. Anal. 25:289–295, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057617 Sat, 01 Jan 2011 00:00:00 +0100 5057617 http://www.medworm.com/index.php?rid=5057616&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20470 Conclusion: The method chosen by a laboratory to diagnose FVL not only depends on the available technical expertise and equipment, but also the type, variety, and extent of other genetic disorders being diagnosed. J. Clin. Lab. Anal. 25:259–288, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057616 Sat, 01 Jan 2011 00:00:00 +0100 5057616 http://www.medworm.com/index.php?rid=5057615&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20469 In this study, the levels of ischemia‐modified albumin (IMA) in pediatric oncology patients with soft tissue sarcomas (STSs) and neuroblastoma (NB) were analyzed. To date, there have been no studies concerning IMA in these groups of patients. Ninety‐nine children with STSs and NB were analyzed from 2006 to 2009, and 30 healthy children were also enrolled in the study. IMA levels were measured throughout treatment in all patients. The levels of IMA in all cancer patients (mean 116.8±39.3 U/ml), in patients with STSs (mean 119.8±27.5 U/ml), and in patients with NB (mean 114.6±36.6 U/ml) were significantly higher than in the control patients (mean 87.3±38.3 U/ml; P=0.0013, 0.0066, and 0.0164, respectively). IMA levels increased before and during the treatment compared with lev... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057615 Sat, 01 Jan 2011 00:00:00 +0100 5057615 http://www.medworm.com/index.php?rid=5057614&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20468 Conclusions: Our results strongly suggest that the determination of IDUA activity using a DBS assay is a secure tool for MPS I diagnosis. However, it is extremely important to assure that all recommendations for collection, transport, and storage are correctly followed to guarantee the quality of the samples. J. Clin. Lab. Anal. 25:251–254, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057614 Sat, 01 Jan 2011 00:00:00 +0100 5057614 http://www.medworm.com/index.php?rid=5057613&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20467 Conclusions: Determination of serum Trp and Tyr concentrations can be employed to assist the diagnosis of the histotypes of lung cancer and tumor stage. Tyr and Trp as indexes on the lung cancer diagnostic sensitivity, specificity were 54.9, 62.9% and 82.4, 92.1%, Trp is an important and special index for lung cancer diagnosis of which the specificity of diagnosis of lung cancer is more than 92%. J. Clin. Lab. Anal. 25:246–250, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057613 Sat, 01 Jan 2011 00:00:00 +0100 5057613 http://www.medworm.com/index.php?rid=5057612&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20466 We describe here the case of urinary sample adulteration with exogenous buprenorphine (6,952 ng/ml), which has led to afalse‐positive immunoassay test result (14.9 ng/ml) on a subsequent sample due to a phenomenon of instrumental carry‐over. This unusual case confirms the importance to take into account adulteration when screening urines for buprenorphine in patients undergoing substitution therapy for opioid dependence, routinely perform a confirmation assay on positive samples, and rule out instrumental carry‐over. J. Clin. Lab. Anal. 25:244–245, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057612 Sat, 01 Jan 2011 00:00:00 +0100 5057612 http://www.medworm.com/index.php?rid=5057611&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20465 Conclusions: We found significantly higher TGF‐β1 levels in lung cancer patients than in healthy individuals. We propose the measurement of serum TGF‐β1 levels as a complementary diagnostic test in lung cancer detection. J. Clin. Lab. Anal. 25:238–243, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057611 Sat, 01 Jan 2011 00:00:00 +0100 5057611 http://www.medworm.com/index.php?rid=5057610&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20464 In conclusion, SAA rises in early stages of NEC and may aid in diagnosis as a serum marker. J. Clin. Lab. Anal. 25:233–237, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057610 Sat, 01 Jan 2011 00:00:00 +0100 5057610 http://www.medworm.com/index.php?rid=5057609&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20463 Conclusion: Type 2 diabetic patients can have significantly raised concentrations of urinary IgLCs before microalbuminuria or renal disease occurs. Further investigations are recommended to assess LC evaluation in the early management of diabetic renal disease. J. Clin. Lab. Anal. 25:229–232, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=5057609 Sat, 01 Jan 2011 00:00:00 +0100 5057609 http://www.medworm.com/index.php?rid=4820496&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20462 Conclusions: Erythrocytosis and severe microcytosis, together with a high percentage of microcytes and a moderate increase in IRF, is the profile of β‐thalassemia carriers, whereas anisocytosis and the hypochromic subset correlates with the severity of the anemia in iron‐deficient patients. J. Clin. Lab. Anal. 25:223–228, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820496 Sat, 01 Jan 2011 00:00:00 +0100 4820496 http://www.medworm.com/index.php?rid=4820495&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20461 This study aimed at comparing detection of intestinal parasites from single unpreserved stool sample vs. sodium acetate acetic acid formalin (SAF)‐preserved pooled samples, and stained with chlorazol black dye in routine practice. Unpreserved samples were collected from 120 patients and represented as Group I. Other three SAF‐preserved samples were collected from the same patients over a 6‐day period and represented as Groups IIa, IIb, and IIc. The latter groups were equally subdivided into two subgroups. The first subgroup of each of the three samples was examined individually, whereas the second subgroup of each were pooled and examined as a single specimen. All groups were examinedby the routine diagnostic techniques; however, in group II when the diagnosis was uncertain, the chlo... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820495 Sat, 01 Jan 2011 00:00:00 +0100 4820495 http://www.medworm.com/index.php?rid=4820494&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20460 In this study, we compared carbamazepine values in samples containing carbamazepine and the epoxide metabolite, as determined by liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) and by two commercial carbamazepine immunoassays: the PETINIA and the ADVIA Centaur carbamazepine. Clinical specimens were used for the comparative studies wherein we determined carbamazepine concentrations using the PETINIA, ADVIA Centaur, and LC‐MS/MS assays. We observed an excellent correlationbetween carbamazepine concentrations determined by the ADVIA Centaur and LC‐MS/MS methods while carbamazepine values were overestimated using the PETINIA assay. When aliquots of drug‐free serum were supplemented with clinically relevant concentrations of the carbamazepine epoxide metabolite, we ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820494 Sat, 01 Jan 2011 00:00:00 +0100 4820494 http://www.medworm.com/index.php?rid=4820493&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20459 AbstractUnited States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent sirolimus concentration obtained by using Architect sirolimus immunoassay and mathematical equations (both polynomial and linear). Mathematical equations were derived by curve‐fitting methods based on observed apparent sirolimus concentration and true everolimus concentration determined by a liquid chromatography combined with mass spectrometry (LC/MS) method using eight everolimus standards (concentration range 1–30 ng/mL) prepared in whole blood. In order to det... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820493 Sat, 01 Jan 2011 00:00:00 +0100 4820493 http://www.medworm.com/index.php?rid=4820492&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20458 AbstractIn the past few years, because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens, serum separator tubes gained widespread acceptance gradually in China. However, some limitations associated with gel tubes had been observed, for example, gel and analyte stability. In order to circumvent these problems, a new tube (BD Vacutainer® SST™ II Plus (BD SST™ II Plus)) containing a new gel was released by BD with respect to analyte and gel stability. We investigated the performance of BD SST™ II Plus tubes for special proteins testing using BD Vacutainer® Serum Glass Tubes (BD Serum Glass) as controls.Equivalence between these two types of tubes was demonstrated for all analytes at initial time, and data for all analyt... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820492 Sat, 01 Jan 2011 00:00:00 +0100 4820492 http://www.medworm.com/index.php?rid=4820491&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20457 AbstractWestergren method is considered as the reference procedure to measure Erythrocyte Sedimentation Rate (ESR) by the International Council for Standardization in Haematology. However, a closed automated method, VES Matic Cube 80 (DIESSE S.p.A., Siena, Italy), has been introduced as a new ESR measurement instrument. In this article, we report two different studies: first, we compared the two methods (Westergren and VES Matic Cube 80) and second, we correlated the inflammatory state of 248 patients with their ESR values. Total protein, albumin, C‐reactive protein, and other inflammatory proteins were detected in each sample. The results obtained using VES Matic Cube 80demonstrated a good correlation with those obtained using the Westergren method (Ordinary linear regression: y=0.955x�... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820491 Sat, 01 Jan 2011 00:00:00 +0100 4820491 http://www.medworm.com/index.php?rid=4820490&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20456 This study enrolled 103 stable CKD patients (stage 3–5 and hemodialysis (HD) patients). Serum levels of indoxyl sulfate and p‐cresylsulfate were measured using ultra performance liquid chromatography. General laboratory results and patient background were also checked. Patients with advanced CKD had higher serum indoxyl sulfate, p‐cresylsulfate based on ANOVA test. There were significant correlation between indoxyl sulfate and p‐cresylsulfate and serum creatinine after multivariate regression analysis (B=3.59, P<0.01; B=0.93, P=0.04, respectively). In addition, there was a positive correlation between indoxyl sulfate and p‐cresylsulfate level (r=0.61, P<0.01). Indoxyl sulfate and p‐cresylsulfate level increased gradually while renal function declined and reached the peak ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820490 Sat, 01 Jan 2011 00:00:00 +0100 4820490 http://www.medworm.com/index.php?rid=4820489&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20454 Conclusions: Based on the results obtained from this study to overcome the problems of venipuncture frozen liquid blood sampling, replacement of a reliable filter paper for preserving finger‐prick blood samples is a trustable and useful facilitator particularly in remote malaria‐endemic areas. J. Clin. Lab. Anal. 25:185–190, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820489 Sat, 01 Jan 2011 00:00:00 +0100 4820489 http://www.medworm.com/index.php?rid=4820488&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20453 AbstractMucopolysaccharidoses (MPS) are a group of lysosomal storage disorders, characterized by the deficiency/absence of one of the enzymes involved in the intralysosomal degradation of glycosaminoglycans (GAGs). The quantitative determination of urinary GAGs using dimethylmethylene blue (DMB) shows high reliability. However, the logistics and staff for this method are not always available in primary care centers. Sending urine samples to reference laboratories increases the cost and delays the diagnosis. Thus, the aim of this article is to develop and evaluate a simple and low‐cost visual test (GAG‐test®) for the screening of urine samples from patients under suspicion of suffering from MPS. The purpose is to narrow down the number of samples to be assayed through the quantitative ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820488 Sat, 01 Jan 2011 00:00:00 +0100 4820488 http://www.medworm.com/index.php?rid=4820487&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20452 Conclusion: Estrogen deficiency alone may not cause an increase in ADMA levels unless the women are prone to disturbances in energy homeostasis. In spite of the high ADMA levels, the unaltered NO levels in plasma may be owing to ongoing inflammatory conditions. J. Clin. Lab. Anal. 25:174–178, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820487 Sat, 01 Jan 2011 00:00:00 +0100 4820487 http://www.medworm.com/index.php?rid=4820486&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20451 In this study, we analyzed the effect of formalin fixation length and autolysis period before fixation/freezing on the expression of several reference genes in formalin‐fixed paraffin‐embedded (FFPE) colorectal carcinoma cells and tissue. We also used a factorial experimental design to further analyze the interaction of both variables. It was found that mRNA levels can be reproducibly quantified, independent of the tested variables. Our findings confirm that clinical tumor specimens removed by routine surgical procedures and combined with real‐time RT‐PCR can be used for the analysis of gene expression in routine FFPE materials and provide some useful information related to the achieved tissues largely stored in histopathology departments. J. Clin. Lab. Anal. 25:166–173, 2011. ©... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820486 Sat, 01 Jan 2011 00:00:00 +0100 4820486 http://www.medworm.com/index.php?rid=4820485&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20450 Conclusion: We suggest that MPV might help in the assessment of fibrosis in CHB. It should not be considered a stand‐alone test for this use owing to nonspecificity with other diseases. J. Clin. Lab. Anal. 25:162–165, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820485 Sat, 01 Jan 2011 00:00:00 +0100 4820485 http://www.medworm.com/index.php?rid=4820484&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20449 Conclusions: There is an increased prevalence of IgA antitissue transglutaminase antibodies, which suggests the need to use this method as an effective first‐line test in the screening of celiac disease in children with type 1 diabetes mellitus. J. Clin. Lab. Anal. 25:156–161, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820484 Sat, 01 Jan 2011 00:00:00 +0100 4820484 http://www.medworm.com/index.php?rid=4820483&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20435 Conclusions: Serum NT‐proBNP was not only a good biomarker to effectively evaluate heart function but also to evaluate the cardiac valve damage in RHD patients, and postoperative NT‐proBNP dynamic determination, especially detecting postoperative 1 day, 7 days and 14 days NT‐proBNP would be more useful to evaluate prognosis of CVR patients. J. Clin. Lab. Anal. 25:149–155, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4820483 Sat, 01 Jan 2011 00:00:00 +0100 4820483 http://www.medworm.com/index.php?rid=4600310&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20448 AbstractTo detect with whole‐cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme‐only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1 hr protocol is a useful alternative to conventional FISH. J. Clin. Lab. Anal. 25:142–147, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600310 Sat, 01 Jan 2011 00:00:00 +0100 4600310 http://www.medworm.com/index.php?rid=4600309&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20447 In this study, a modified enzyme immunoassay (EIA) was evaluated for the Hepatitis B surface antigen (HBsAg) among whole saliva and oral fluid samples. Specimens were collected from 115 individuals who gave serum and oral fluid using Salivette (Sarstedt, Nümbrecht, Germany) and whole saliva. Saliva specimens were tested following a modified ELISA, and the results were compared with paired serum specimens that were tested according to the supplier's instructions. Transport buffer for the oral fluids, sample volume for assay, incubation period of sample with conjugate as well as cut‐off values were evaluated to optimize the assay. The highest sensitivity and specificity were obtained by increasing the incubation of sample and conjugate to 16 hr and using the area under the receiver oper... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600309 Sat, 01 Jan 2011 00:00:00 +0100 4600309 http://www.medworm.com/index.php?rid=4600308&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20446 Conclusion: This study has objectively shown that the analytical precision of current instruments is being achieved contrary to the known problems surrounding the analytical bias for 25D assays. The limitations of using reference intervals for 25D, both in diagnoses and monitoring are shown. J. Clin. Lab. Anal. 25:130–133, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600308 Sat, 01 Jan 2011 00:00:00 +0100 4600308 http://www.medworm.com/index.php?rid=4600307&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20445 Conclusions: Our data can be used as a reference for the assessment of carnitine status in Indian newborns, hence reducing the risk of misdiagnosis of fatty acid oxidation disorders and organic acidemias during interpretation of the results of tandem mass spectrometry‐based newborn screening. J. Clin. Lab. Anal. 25:126–129, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600307 Sat, 01 Jan 2011 00:00:00 +0100 4600307 http://www.medworm.com/index.php?rid=4600306&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20444 AbstractFlow cytometric analysis of leukocyte surface antigens has been used to characterize infectious and septic processes in patients. We wanted to investigate how the sampling and processing temperature, the anticoagulant used, and the storage of the sample influence leukocyte immunophenotyping. Four blood samples, two using acid citrate dextrose and two using heparin as an anticoagulant, were taken from five intensive‐care unit patients with severe sepsis and five healthy volunteers. The samples were collected, stored, and processed either at +4°C or at room temperature (RT). The samples were processed for flow cytometric analysis within 1 hr of collection or after 6 or 24 hr storage. The surface antigens of interest were neutrophilic CD11b and CD64, monocytic CD11b, CD14, CD40... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600306 Sat, 01 Jan 2011 00:00:00 +0100 4600306 http://www.medworm.com/index.php?rid=4600305&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20443 In conclusion, the sensitivity of HCC diagnosis can be improved by combined detection of AFP and GPC3 mRNA expressions. GPC3 mRNA is HCC‐specific, and may indicate HCC metastasis. J. Clin. Lab. Anal. 25:113–117, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600305 Sat, 01 Jan 2011 00:00:00 +0100 4600305 http://www.medworm.com/index.php?rid=4600304&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20442 Conclusion: An increased serum Hcy may be a precipitating factor for vitiligo in the predisposed individuals. Serum Hcy is related to the gender of patients, activity of disease, and dietary habits of vitiligo patients. J. Clin. Lab. Anal. 25:110–112, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600304 Sat, 01 Jan 2011 00:00:00 +0100 4600304 http://www.medworm.com/index.php?rid=4600303&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20441 AbstractOleander poisoning can be detected by digoxin immunoassays and for last two decades the fluorescence polarization immunoassay (FPIA) has been used for rapid detection of oleander poisoning in clinical laboratories. Recently, Abbott Laboratories (Abbott Park, IL) discontinued this assay. Therefore, we explored the possibility of using another digoxin assay (Dimension Vista Flex Reagent Cartridge, Tina Quant, EMIT 2000 and old FPIA assay for comparison) for rapid detection of oleander poisoning. When aliquots of drug‐free serum pools were supplemented with pure oleandrin or oleander extract, we observed the highest apparent digoxin values using Dimension Vista digoxin assay (Flex Reagent Cartridge). We also observed significant apparent digoxin values in vivo in sera of mice both 1... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600303 Sat, 01 Jan 2011 00:00:00 +0100 4600303 http://www.medworm.com/index.php?rid=4600302&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20440 Conclusion: Our findings indicate that the accuracy of RHD gene using three regions (exons 4, 5, and 10) can be sufficient for clinical application in a multi‐ethnic population. This knowledge helped us on the development of a feasible protocol for fetal RHD genotyping on DNA from maternal plasma for our population. J. Clin. Lab. Anal. 25:100–104, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600302 Sat, 01 Jan 2011 00:00:00 +0100 4600302 http://www.medworm.com/index.php?rid=4600301&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20439 AbstractTwo methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one‐step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine‐thiocyanate‐based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed by column‐based purification coupled with one‐step RT‐PCR proved to be a simple, safe, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of both types of VSV in cell lysate and spiked tissue samples as compared with the tri‐phasic extraction method (Tri‐reagent method). When RNA was extracted either from VSV cell culture stock or from VSV spiked bovine lymph nodes by using Qiagen RNeasy Mini Kit, the detecti... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600301 Sat, 01 Jan 2011 00:00:00 +0100 4600301 http://www.medworm.com/index.php?rid=4600300&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20438 In this study, we evaluated a new technique for the automatic cell viability measurement with microscopic cell counter and microchip. Upon blood draw from 11 healthy volunteers, Mononuclear cells were separated immediately from the heparinized whole blood, and the viable cells were diluted from 100 to 1%. The cell viability tests were performed simultaneously with following three methods: the conventional manual trypan blue exclusion method; the flow cytometry measurement with propidium iodide stain; and the newly developed microscopic cell counter with microchip. Linearities, precisions, and correlations from three methods were analyzed and compared. The correlations data from the microscopic cell counter were in good agreement with both the conventional trypan blue method (r=0.99, P<0... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600300 Sat, 01 Jan 2011 00:00:00 +0100 4600300 http://www.medworm.com/index.php?rid=4600299&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20437 Conclusion: The ATE/LER zones method is a relatively reasonable and widely considering method for assessing the agreement. J. Clin. Lab. Anal. 25:83–89, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600299 Sat, 01 Jan 2011 00:00:00 +0100 4600299 http://www.medworm.com/index.php?rid=4600298&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20436 Conclusion: Molecular screening of Brazilians revealed one novel allele, the DO*A‐WL. Our data highlight the importance of testing a cohort of different populations to determine DO haplotypes and to establish reliable genotyping tests for predicting Doa/Dob status. J. Clin. Lab. Anal. 25:79–82, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600298 Sat, 01 Jan 2011 00:00:00 +0100 4600298 http://www.medworm.com/index.php?rid=4600297&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20434 This study has evaluated the clinical applicability of a single‐tube multiplex RT‐ PCR as compared with a two‐step nested RT‐PCR for the diagnosis as well as serotyping of dengue virus in patient's samples. Seventy‐six acute phase blood samples collected from clinically suspected dengue patients during the 2008 outbreak were subjected to two‐step nested RT‐PCR and single‐tube multiplex RT‐PCR for dengue diagnosis and serotyping. Of the 76 samples, 17 (22.4%) were positive for dengue viral RNA. Single dengue virus infection was found in 16 cases and 1 had concurrent infection with two serotypes (3&1). Dengue serotype 3 was the predominant serotype (70.5%), followed by serotype 1 (23.5%). Single‐tube multiplex PCR had concordant result with that of two‐step nested R... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600297 Sat, 01 Jan 2011 00:00:00 +0100 4600297 http://www.medworm.com/index.php?rid=4600296&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20433 This study was aimed to evaluate the analytical performance of an automated image analysis system (a pilot model of Diff Master™ Octavia) for the preclassification of leucocytes in children with hematological disease. Manual microscopy performed by pediatric hematologists was used as the reference method. Five mature cell class and blasts were evaluated. Diff Master Octavia correctly preclassified 87.4% of all leucocytes with a high reproducibility. The overall accuracy was found to be 93.0%. Clinical sensitivity was 97.7% and specificity was 76.0%. The average time per slide for Diff Master™ Octavia was 2.3 min lower than that of manual method. Our results indicated that the Diff Master™ Octavia can detect and preclassify leucocytes accurately; therefore, it can be used as an effi... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600296 Sat, 01 Jan 2011 00:00:00 +0100 4600296 http://www.medworm.com/index.php?rid=4600295&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20425 AbstractCongenital disorder of glycosylation type Ic (CDG‐Ic) is caused by mutations in hALG6 gene encoding α‐1,3 glucosyltransferase (NP_037471.2), an enzyme that catalyzes the addition of the first glucose residue to the growing lipid‐linked oligosaccharide precursor in N‐glycosylation process. The most frequent mutation in hALG6 gene causing CDG‐Ic is c.998C>T that results in p.A333V substitution. Up‐to‐date, no CDG‐Ic patient has been detected in Croatia. However, as a part of the comprehensive project undertaken with the aim to estimate the frequencies of the carriers for specific mutations and polymorphisms related to particular CDGs in Croatian population, we screened genomic DNA samples obtained from 600 healthy nonconsanguineous Croatian residents to determine ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4600295 Sat, 01 Jan 2011 00:00:00 +0100 4600295 http://www.medworm.com/index.php?rid=4371633&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20432 (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371633 Sat, 01 Jan 2011 00:00:00 +0100 4371633 http://www.medworm.com/index.php?rid=4371632&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20431 Conclusion: Results showed that the programs based on the primary blood TSH/back up TSH measurements improve the thyroid screening results. J. Clin. Lab. Anal. 25:61–63, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371632 Sat, 01 Jan 2011 00:00:00 +0100 4371632 http://www.medworm.com/index.php?rid=4371631&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20430 AbstractMany laboratories rely on dedicated nephelometers and turbidimeters for the measurement of serum proteins. There are, however, a number of chemistry analyzers that offer open channel configurations for end‐user applications. We developed and validated 14 human serum protein assays (α1‐antitrypsin, α2‐macroglobulin, albumin, apolipoproteins AI and B, complement components 3 and 4, haptoglobin, immunoglobulins A, G, and M, orosomucoid, transferrin, and transthyretin) on the Roche cobas® c 501. We obtained excellent precision at low, normal, and high physiologic concentrations of each protein (within‐run imprecision CVs ≤2.5%, total imprecision CVs ≤3.6%). Linearity for each method was within 5% of the expected value throughout the calibration range, and method comparis... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371631 Sat, 01 Jan 2011 00:00:00 +0100 4371631 http://www.medworm.com/index.php?rid=4371630&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20429 Conclusion: Phagocytosis and ·NO production are affected by rheumatic states. This suggests that the increased ·NO levels may play a part in the increased oxidative stress in rheumatic diseases in elderly women. J. Clin. Lab. Anal. 25:47–51, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371630 Sat, 01 Jan 2011 00:00:00 +0100 4371630 http://www.medworm.com/index.php?rid=4371629&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20428 Conclusion: Our results suggest that APOE‐ε4 is a risk factor for stenosis but does not has any effect on the severity of this disease. J. Clin. Lab. Anal. 25:43–46, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371629 Sat, 01 Jan 2011 00:00:00 +0100 4371629 http://www.medworm.com/index.php?rid=4371628&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20427 Conclusions: The bead‐based platform is rapid, sensitive, and less expensive, which allows both single sample and high‐throughput measurement of tPSA and fPSA over a wide range of concentrations. J. Clin. Lab. Anal. 25:37–42, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371628 Sat, 01 Jan 2011 00:00:00 +0100 4371628 http://www.medworm.com/index.php?rid=4371627&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20403 We report here clarithromycin‐resistant clinical isolates of M. avium with the different characteristics from those of the resistant strains reported from earlier studies. J. Clin. Lab. Anal. 25:33–36, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371627 Sat, 01 Jan 2011 00:00:00 +0100 4371627 http://www.medworm.com/index.php?rid=4371626&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20381 In this study, we evaluated the efficacy of serum IgG antibody titer to periodontal bacteria for prognosis of periodontitis recurrence during supportive periodontal therapy (SPT) phase. The 139 patients during SPT phase were selected and divided to two groups as follows: “Stable” and “Recurrence” group at SPT phase for case–control study: “High IgG titer” and “Normal IgG titer” group before transition to SPT phase for cohort study. We examined whether clinical findings or serum IgG antibody titers to periodontal bacteria are risk factors for the development of periodontitis recurrence. Case–control study showed thatthere were significant differences between the stable and recurrence groups in age and number of teeth. The serum IgG antibody titer to Eikenella corrodens F... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371626 Sat, 01 Jan 2011 00:00:00 +0100 4371626 http://www.medworm.com/index.php?rid=4371625&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20377 This study was aimed at identifying the Leishmania species using serosity materials punctured from skin lesions of cutaneous leishmaniasis (CL) patients by using internal transcribed spacer1 (ITS1) polymerase chain reaction (PCR)‐restriction fragment length polymorphism (RFLP). We used the PCR‐RFLP on 60 parasitological confirmed CL patients who referred to leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. The PCR‐RFLP could correctly detect 51 Leishmania species of the 60confirmed positive specimens, where all the other 10 parasitological (microscopy and culture) negative samples that were prepared from other bacterial‐ and fungal‐infected lesions had negative results. The results also revealed that Leishmania major was the dominan... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371625 Sat, 01 Jan 2011 00:00:00 +0100 4371625 http://www.medworm.com/index.php?rid=4371624&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20376 AbstractWe assessed the characteristics of the new semi‐quantitative test paper (Clinitek ATLAS Pro12) using random urine samples. Three hundred urine samples were analyzed using eitherthe new test paper, conventional dipsticks, quantitative (P/C ratio), or immunological quantitative methods (A/C ratio). Our study showed that the new test paper is highly sensitive and specific for the detection of urinary protein. The new test paper also detected the urine protein more accurately than the conventional test and has a lower false‐positive rate. In addition, the new test paper detected 14 of the 300 patients (4.7%) as dilute urine samples needing reassessment. Seventeen of the 300 samples tested were negative with conventional dipsticks but positive with the new test paper. The new semi�... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371624 Sat, 01 Jan 2011 00:00:00 +0100 4371624 http://www.medworm.com/index.php?rid=4371623&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20347 Conclusions: The results suggest that oxidative–antioxidative balance and collagen turnover are altered by the development of COPD in human lungs, and prolidase activity may reflect disturbances of collagen metabolism in this pulmonary disease. Monitoring of plasma prolidase activity and oxidative–antioxidative balance may be useful in evaluating fibrotic processes and oxidative damage in the chronic inflammatory lung disease in human. J. Clin. Lab. Anal. 25:8–13, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371623 Sat, 01 Jan 2011 00:00:00 +0100 4371623 http://www.medworm.com/index.php?rid=4371622&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20411 AbstractIntervention by membrane KCC transporter interfering selectively could promote about 5 times enrichment of nuclear red blood cells. J. Clin. Lab. Anal. 25:1–7, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4371622 Sat, 01 Jan 2011 00:00:00 +0100 4371622 http://www.medworm.com/index.php?rid=3746578&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20400 Carbamazepine is an anticonvulsant requiring routine therapeutic drug monitoring. Recently, Siemens Healthcare Diagnostic Division released a new carbamazepine assay: ADVIA Chemistry Carbamazepine_2 (Carbamazepine_2) for application on ADVIA analyzers. We evaluated the analytical performance of this assay as well as its potential cross-reactivities with carbamazepine 10, 11-epoxide, hydroxyzine, and cetirizine. The within-run and between-run precisions of the Carbamzepine-2 assay were (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746578 Sun, 11 Jul 2010 23:00:00 +0100 3746578 http://www.medworm.com/index.php?rid=3746577&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20399 Ischemia-Modified albumin (IMA) has been used as an early marker in the evaluation of the patients with acute coronary syndrome. We aimed to evaluate IMA in end-stage renal disease (ESRD) patients on hemodialysis and the effect of albumin methods on albumin-adjusted IMA levels. A total of 30 ESRD patients were included in this study. Serum IMA and albumin levels were measured before and after a hemodialysis session. Albumin concentrations were determined with bromocresol green and bromocresol purple methods. Postdialysis IMA and albumin-adjusted IMA levels with two different albumin methods were significantly increased compared with the predialysis levels (P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746577 Sun, 11 Jul 2010 23:00:00 +0100 3746577 http://www.medworm.com/index.php?rid=3746576&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20398 Etiopathology of psoriasis is not completely understood. Patients with psoriasis show elevated sensitivity to gluten. The aim of this study was to see the expression of celiac disease (CD)-associated antibodies gliadin IgA, gliadin IgG, and tissue transglutaminase IgA, and their correlation with HLA Cw6 in patients with psoriasis. The study comprised 56 patients with psoriasis and 60 healthy controls (HC). The levels of antibodies were detected by using ELISA technique and HLA Cw6 typing was carried out by microcytotoxicity method. HLA Cw6 was significantly expressed in psoriasis cases when compared with HC (P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746576 Sun, 11 Jul 2010 23:00:00 +0100 3746576 http://www.medworm.com/index.php?rid=3746575&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20409 Conclusion: BAT2 -8671, BAT3 8854, and BAT5 22655, 9569 polymorphisms as well as BAT haplotypes (ATTGTG and ATCATG) might be associated with higher KD susceptibility and CAA formation. HLA-B region polymorphisms might contribute to the pathogenesis of KD and CAA. J. Clin. Lab. Anal. 24:262-268, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746575 Sun, 11 Jul 2010 23:00:00 +0100 3746575 http://www.medworm.com/index.php?rid=3746574&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20395 Previous studies have shown elevated serum levels of interleukin 6 (IL-6) and C-reactive protein (CRP) in patients with pancreatic cancer (PC). The aim of this study was to assess the diagnostic usefulness of pretreatment serum levels of IL-6 and CRP to differentiate between PC and chronic pancreatitis (CP) patients. Serum levels of CRP, IL-6, carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA 19-9) were determined in 78 patients with PC before surgery, in 45 patients with CP, and in 70 healthy controls. Serum levels of all the proteins tested were significantly higher in cancer patients when compared with CP and healthy subjects, and increased in more advanced tumor stages. Concentrations of IL-6 were significantly higher in nonresectable tumors and in patients who died dur... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746574 Sun, 11 Jul 2010 23:00:00 +0100 3746574 http://www.medworm.com/index.php?rid=3746573&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20394 In conclusion, we can affirm that total automation of reticulocyte counts represents a definite improvement over microscopic counts. This study confirms the diversity of the reference intervals still exists in the new automated hematology analyzers. J. Clin. Lab. Anal. 24:252-255, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746573 Sun, 11 Jul 2010 23:00:00 +0100 3746573 http://www.medworm.com/index.php?rid=3746572&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20393 Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix (ECM) in the arterial wall during atherogenesis. Collagens are the most abundant proteins in the ECM. MMP-8 is expressed by cells associated with the development of the atherosclerotic plaque. It cleaves collagen type I three times more potently than two other interstitial collagenases MMP-1 and MMP-13. The aim of this study was to investigate whether plasma MMP-8 values are associated with occurrence of carotid plaque (CP) and possible correlations with clinical and biochemical parameters in carotid atherosclerosis (CA) patients. Total plasma MMP-8 levels were quantified by ELISA in 63 patients with ultrasonographic evidence of CP presence and 12 controls. Plasma MMP-8 values were significantly hig... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746572 Sun, 11 Jul 2010 23:00:00 +0100 3746572 http://www.medworm.com/index.php?rid=3746571&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20392 Oxidative stress is an unavoidable event during many complex surgical procedures. 8-iso-prostaglandin F2[alpha] (8-iso-PGF2[alpha]) is a reliable biomarker for the evaluation of oxidative stress in vivo. The aim of this study is to develop simple and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the detection of urinary 8-iso-PGF2[alpha] in samples collected from patients who received a cardiopulmonary bypass (CPB) during cardiac valve surgery. Urine samples of 14 patients with cardiac valve diseases were collected before, during, and after CPB. The level of 8-iso-PGF2[alpha] was detected via selected-reaction monitoring triple quadrupole MS/MS and the result was compared with 12 healthy volunteers. The method's detection limit (3S/N) was 0.25 pg for 8-iso-... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746571 Sun, 11 Jul 2010 23:00:00 +0100 3746571 http://www.medworm.com/index.php?rid=3746570&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20391 Detection of antinuclear antibodies (ANA) is a diagnostic adjunct in patients with suspected autoimmune connective tissue diseases, and various detection methods are in use. The aim of this study was to analyze the agreement between the ANA immunoflourescence (IF) and immunoblotting (IB) methods and determine cut-off for children subjects in a laboratory setting. We evaluated 729 serum samples that were analyzed by both ANA IF and IB. The results were evaluated by [chi]2 test and, for agreement, [kappa] index was used. Frequencies determined for both 1:40-1:100 cut-off titers of ANA IF in relation to IB testing supported the idea that 1:100 starting dilution should be recommended in children subjects for ANA IF method and antigen specific immunoblot testing was needed, especially for some ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746570 Sun, 11 Jul 2010 23:00:00 +0100 3746570 http://www.medworm.com/index.php?rid=3746569&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20390 Early diagnosis of Mycoplasma pneumoniae (Mp) plays a pivotal role in its management. We evaluated the role of rapid culture method in early diagnosis of Mp infection and discussed the potential impact factors. A total of 2,600 patients with acute respiratory infection were included, and their pharyngeal swab samples were prepared for Mp rapid culture based on selective Mp fluid culture medium. The clinical contributing factors related to Mp infection were also explored. The positive rate of Mp culture in females was 41.75%, which was higher than that for males (37.63%). Mp infections were incidental to the children and elderly. The positive rates of Mp culture were higher in children aged 3-5 years and adults older than 70 years (54.05 and 31.48%, respectively), compared with other ages. ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746569 Sun, 11 Jul 2010 23:00:00 +0100 3746569 http://www.medworm.com/index.php?rid=3746568&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20389 Background: Examination of Exhaled Breath Condensate has been suggested to give information about inflammatory airway diseases. Objectives: The aim was to compare efficacy and variability in gain of two commercially available exhaled breath condensers, ECoScreen® and RTubeTM in an in vitro set up. Methods: Test fluids containing myeloperoxidase (MPO) or human neutrophil lipocalin (HNL) in addition to saline and bovine serum albumin were nebulized and aerosols were transferred by a servo ventilator to either of the two condensers. Analyses of MPO, HNL, or chlorine were done by means of ELISA, RIA, or a modified adsorbed organic halogen technique (AOX), respectively. Results: Recoveries of HNL were higher when using ECoScreen than RTube (P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746568 Sun, 11 Jul 2010 23:00:00 +0100 3746568 http://www.medworm.com/index.php?rid=3746567&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20388 In this study, we measured prolidase enzyme activity in patients with idiopathic or ischemic DCM and in healthy controls, making this, to our knowledge, the first study to do so. What we found is that serumprolidase activity was significantly lower in both DCM groups relative to healthy volunteers and lower in ischemic DCM than idiopathic. These intriguing results could be attributed either to decreased collagen turnover in the heart tissues in which DCM develops, a result of diminished functional heart tissue, or to decreased physical activity levels among DCM patients stemming from their heart failure. Either way, further studies are needed to verify and clarify our results. J. Clin. Lab. Anal. 24:213-218, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3746567 Mon, 31 May 2010 23:00:00 +0100 3746567 http://www.medworm.com/index.php?rid=3563752&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20350 Background: The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic).Methods: Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albumin, glucose, hemoglobin and lipid, and patient sera with maximum concentrations of bilirubin, 1,090 µmol/l and protein, 117.8 g/l.Results: Hemoglobin, 7.5 g/l and lipaemic with triglyceride concentration of 6.27 mmol/l, did not interfere with all assays. Glucose >33.3 mmol/l increased creatinine recovery for Dimension method. Samples spiked with bilirubin imparted a negative bias for Dimension and Architect methods but imparted a positive bias for Vitros assay. However, usi... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563752 Fri, 14 May 2010 15:32:02 +0100 3563752 http://www.medworm.com/index.php?rid=3563766&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20334 The aim of this study was to investigate serum prolidase enzyme activity and to find out its association with liver biopsy specimens' histopathological findings in patients with nonalcoholic steatohepatitis (NASH), which may progress to liver fibrosis and cirrhosis. Thirty-six patients with biopsy-proven NASH and 29 healthy controls were enrolled. Serum prolidase enzyme activity was measured spectrophotometrically. Serum prolidase enzyme activity was significantly higher in patients with NASH thancontrols (P=0.016). A significant correlation was observed between serum prolidase enzyme activity and fibrosis score in patients with NASH (r=0.661, P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563766 Wed, 12 May 2010 23:00:00 +0100 3563766 http://www.medworm.com/index.php?rid=3563765&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20378 Osteopontin (OPN), a bone-related protein, is present within the atherosclerotic plaques, most strikingly in calcified plaques. Valvular calcifications are accepted as a part of the spectrum of atherosclerosis and are associated with atherosclerotic calcification in the coronary arteries. The study aimed to evaluate the association of plasma OPN with the presence and extent of coronary stenosis, mitral annular calcification (MAC), and aortic valve sclerosis in stable angina patients. We studied 120 subjects who underwent coronary angiography because of ischemic chest pain. Coronary artery disease (CAD) was defined as [ge]50% stenosis in [ge]1 coronary artery. MAC and aortic valve sclerosis were detected by echocardiography. Lipid profile, high sensitive C-reactiveprotein (hsCRP), and OPN w... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563765 Wed, 12 May 2010 23:00:00 +0100 3563765 http://www.medworm.com/index.php?rid=3563764&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20320 This study aimed to investigate the association of changes in circulating IGF-1 with hepatocellular carcinoma (HCC). The radioimmunoassay was used to analyze serum IGF-1 levels of 65 HCC patients and 165 healthy subjects. Serum IGF-1 levels were significantly decreased in the HCC patients as compared with the healthy subjects (158.46±105.07 vs. 247.63±149.96 ng/mL, P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563764 Wed, 12 May 2010 23:00:00 +0100 3563764 http://www.medworm.com/index.php?rid=3563763&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20380 Human African trypanosomiasis (HAT), also called African sleeping sickness, is a neglected tropical parasitic disease indigenous to sub-Saharan Africa. Diamidine compounds, including pentamidine and CPD-0801, are potent anti-trypanosomal molecules. The latter is a potential drug in the development at the UNC based Consortium for Parasitic Drug Development. An orally bioavailable prodrug of CPD-0801, DB868, is metabolized primarily in the liver to the active form. A monoclonal antibody developed against a pentamidine derivative has shown significant reactivity with CPD-0801 (EC50 65.1 nM), but not with the prodrug (EC50>18,000 nM). An inhibitory enzyme-linked immunosorbent assay (IELISA) has been used to quantitatively monitor prodrug metabolism by detecting the production of the active com... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563763 Wed, 12 May 2010 23:00:00 +0100 3563763 http://www.medworm.com/index.php?rid=3563762&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20379 In this study, the relationship between genetic variants situated at the PARP-1 gene and AD development was investigated. We performed a case and control study from a Taiwanese population enrolled 120 AD patients and 111 healthy controls by using a polymerase chain reaction restriction fragment length polymorphism approach for two PARP-1 exonic polymorphisms, 414C/T (rs1805404) and 2456T/C (rs1136410), corresponding to protein residues at positions 81Asp/Asp and762Val/Ala. There were no significant differences in allele or genotype frequencies for either PARP-1 gene variant between the case and control groups; however, upon analysis of the haplotype distribution, four haplotypes (Hts) were identified. We found that the distributions of Ht3-TT and Ht4-CC were significantly associated with a... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563762 Wed, 12 May 2010 23:00:00 +0100 3563762 http://www.medworm.com/index.php?rid=3563761&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20384 Conclusion: This multiplex PCR assay offers high sensitivity and improved detection speed relative to culture. The appropriate combination of this new multiplex PCR assay with culture may contribute to the accurate and rapid diagnosis of SA. J. Clin. Lab. Anal. 24:175-181, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563761 Wed, 12 May 2010 23:00:00 +0100 3563761 http://www.medworm.com/index.php?rid=3563760&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20385 Aim: Detection of cytogenetic alterations in esophageal cancer (EC). A total of 40 cases of primary EC and their paired nearby nontumor tissues were collected. The comparative genomic hybridization (CGH) is the technique that brings out the gains and losses of chromosome fragments and was applied to determine the aberrations from the tissue DNA. In noncancer tissues, the gains were at 19p (5/40, 13%), 20q (5/40, 13%), and losses at 9p (13/40, 33%), 2q (10/40, 25%), 12q (10/40, 25%), 13q (10/40, 25%), 5q (9/40, 23%), 6q (9/40, 23%), 7q (9/40, 23%), and 8p (9/40, 23%). Two cases in nontumor tissues showed no CGH change. In the 40 cases of primary EC, the gains were at 8q (10/40, 25%), 3q (9/40, 23%), 2q (7/40, 18%), and 13q (7/40, 18%), and the losses were at 1q (8/40, 20%), 4q (8/40, 20%), ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563760 Wed, 12 May 2010 23:00:00 +0100 3563760 http://www.medworm.com/index.php?rid=3563759&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20383 Conclusion: Laboratory tests including classical parameters (platelet count, PT, aPTT, INR, and fibrinogen) of coagulopathy seem to be enough for the followup of CCHF. Protein S, protein C, APCR, and D-dimer levels were not associated with mortality. J. Clin. Lab. Anal. 24:163-166, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563759 Wed, 12 May 2010 23:00:00 +0100 3563759 http://www.medworm.com/index.php?rid=3563758&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20382 Despite brucellosis having a low incidence rate in developed nations, it still remains the leading zoonotic disease in the world. Culturing of Brucella spp. provides good specificity but in cases where the fever is intermittent, sensitivity is problematic. This has led to the development of serological methods of detection. Brucella agglutination methods have been considered the serological gold-standard since their inception, although commercial Brucella IgG and IgM enzyme-linked immunosorbentassays are available to potentially aid in the diagnosis of the disease. In our study, anti-Brucella IgG and IgM assays were compared with agglutination. Individually the IgG assay tested had an accuracy of 56% and the IgM assay had an accuracy of 77%. These poor accuracies reinforce Centers for Dise... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563758 Wed, 12 May 2010 23:00:00 +0100 3563758 http://www.medworm.com/index.php?rid=3563757&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20374 Inflammation and genetics may play a role in the pathogenesis of febrile seizures (FSs). We aimed to test whether interleukin-1[beta] (IL-1[beta]), IL-1 receptor antagonist (IL-1 Ra), IL-6 promoter, IL-8, IL-10, or tumor necrosis factor (TNF) gene polymorphisms could be used as markers of susceptibility to FSs. An association study was performed among a cohort of 104 patients with FSs and 143 normal control subjects. There was no significant difference between patients and controls in the distribution of allele frequencies of the IL-1[beta] promoter, IL-1[beta] exon 5, IL-6 promoter, IL-8, IL-10, or TNF-[alpha] gene polymorphisms. In contrast, the IL-1 Ra-I homozygote was more frequent in patients with FSs than in healthy controls (93.2% vs. 83.92%, [chi]2=4.51, P=0.034). In addition, indi... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563757 Wed, 12 May 2010 23:00:00 +0100 3563757 http://www.medworm.com/index.php?rid=3563756&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20375 Conclusion: If used in the first tier of a multi-tier screening protocol, the proposed method would allow the detection of abnormal levels of all GAGs except hyaluronic acid. J. Clin. Lab. Anal. 24:149-153, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563756 Wed, 12 May 2010 23:00:00 +0100 3563756 http://www.medworm.com/index.php?rid=3563755&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20373 The aim of this study was to screen for C. dubliniensis using the germ tube test with human pooled serum (HPS) in clinical isolates and investigate the prevalence of C. dubliniensis in Korea. Among 1,854 yeast strains isolated, 1,404 strains of C. albicans (on the basis of positive results of the germ tube test) and 192 germ tube-negative yeast strains were examined. All 1,596 clinical isolates were examined using the germ tube test with HPS, the differential temperature, and NaCl tolerance test. Only 81 isolates that did not grow at 45°C nor on Sabouraud 6.5% NaCl broth were selected and tested using the VITEK 2 ID-YSTsystem and the multiplex-PCR assay for the study. The two strains, C. dubliniensis ATCC MYA-646 and KCTC 17427 failed to produce germ tubes in HPS but produced them in fres... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563755 Wed, 12 May 2010 23:00:00 +0100 3563755 http://www.medworm.com/index.php?rid=3563754&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20396 This study evaluated the alkaline wash/lysis procedure to extract DNA of microorganisms in a clinical blood culture. A multiplex polymerase chain reaction (PCR) targeting the 16S rDNA (ribosomal DNA) gene and the fungal ITS (internal transcribed spacer) gene was used as a reliable indicator for the presence of microorganism DNA in the extracts. A total of 535BacT/ALERT positive blood culture bottles were evaluated. Multiplex PCR showed positive results in 530 DNA extracts, but 5 DNA extracts gave negative results. We conclude that the alkaline wash/lysis procedure in combination with the multiplex PCR is a simple and sensitive method, which can be used in a standard diagnostic laboratory to detect microorganisms in blood culture material. J. Clin. Lab. Anal. 24:139-144, 2010. © 2010 Wiley... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563754 Wed, 12 May 2010 23:00:00 +0100 3563754 http://www.medworm.com/index.php?rid=3563753&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20354 The storage conditions of blood samples for reliable results are very important in hepatitis C virus (HCV) RNA amplification tests used in routine HCV analyses. According to many studies, storage conditions could affect the RNA stability for HCV RNA detection. We have studied HCV RNA stability in blood samples stored at 4°C. Nineteen blood samples containing different HCV RNA levels were stored at 4°C and they were then analyzed by TaqMAN real-time PCR method. HCV RNA levels remained almost stable (100%) at least for five weeks at this storage condition. However, among them, the stability period was up to 11 weeks in two of the samples. As with these findings, there was a slightly significant correlation between the positivity time and the beginning HCV RNA levels (r=0.474, P=0.040). We ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3563753 Wed, 12 May 2010 23:00:00 +0100 3563753 http://www.medworm.com/index.php?rid=3398717&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20352 Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed as a suitable nucleic acid target. The gene was detected in all 17 S. lugdunensis isolates examined, while no amplification product was obtained from 98 isolates representing 11 staphylococcal and 17 nonstaphylococcal species. Forty-seven percent of the S. lugdunensis strains produced a positive slide coagulase reaction, which is consistent with varying levels of Fbl protein expression within the species. J. Clin. Lab. Anal. 24:119-122, 2010. © 2010 Wiley-Liss, Inc. (Source: ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398717 Tue, 23 Mar 2010 00:00:00 +0100 3398717 http://www.medworm.com/index.php?rid=3398716&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20372 In conclusion, in Japanese patients, there is evidence implicating C3 concentration as a marker of Mets coinciding with insulin resistance. J. Clin. Lab. Anal. 24:113-118, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398716 Tue, 23 Mar 2010 00:00:00 +0100 3398716 http://www.medworm.com/index.php?rid=3398715&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20371 We describe the history and current implementation of an inexpensive thin layer chromatography (TLC) method, vertical sandwich-type continuous/evaporative TLC with fixed mobile phase volume, that is convenient for detecting and identifying reducing sugars of clinical relevance in the paper-borne blood and urine samples collected in neonatal screening programmes. This method facilitates screening by providing a considerable degree of standardization of chromatographic results. Among some 555,000 newborns to which it has been applied, it has detected 10 cases of classical galactosaemia, 7 cases of galactokinase deficiency, 2 cases of glucosuria, and 3 cases of transitory neonatal diabetes mellitus; the only false negatives we are aware of were two cases of galacto-4-epimerase deficiency dete... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398715 Tue, 23 Mar 2010 00:00:00 +0100 3398715 http://www.medworm.com/index.php?rid=3398714&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20368 In this study, we examined the relationships between baseline values of parameters related to physical activity and known markers of CV disease, including markers of oxidative stress. Methods: A total of 455 healthy men and women between the ages of 18 and 50 were recruited to participate in the study. Levels of lipids/lipoproteins and markers of oxidative stress and inflammation were measured along with the VO2 and duration time spent on treadmill. Results: Women, in general, had a significantly (P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398714 Tue, 23 Mar 2010 00:00:00 +0100 3398714 http://www.medworm.com/index.php?rid=3398713&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20362 In this study, we made an artificial stone composition and examine under IR absorbance by mixing equal molar ratios of melamine/cyanuric acid with different types of human urinary stones, and established a reference of IR analysis for the identification of melamine/cyanuric acid-containing human urinary tract stones. Knowledge of the precise stone composition allowed institution of appropriate prophylactic dietary and medical therapy and this may help in the prevention of urinary stone recurrence. The results are promising that melamine and cyanuric acid can be identified clearly in a low percentile ([sim]1%) of stone mixture pellet. Therefore, IR seems to be an ideal tool for the identification of melamine/cyanuric acid-containing stones. J. Clin. Lab. Anal. 24:92-99, 2010. © 2010 Wiley-... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398713 Tue, 23 Mar 2010 00:00:00 +0100 3398713 http://www.medworm.com/index.php?rid=3398712&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20359 We have developed a new method based on specific primer extension reactions coupled with plate hybridization for high-throughput genotyping of single-base mutations. To improve the switching characteristics of the primer extension reaction, we introduced an artificial mismatch two bases upstream of the 3[prime]-terminal base in the detection primers. A set of primers that correspond to wild-type and mutant DNA segments can be used to accurately analyze single-base mutations. The termini of these primers are at the mutation positions. The primer extension products produced by polymerase chain reaction (PCR) were captured by an oligonucleotide probe immobilized on the surface of microtiter wells and were detected by a colorimetric assay using the streptavidin-conjugated horseradish peroxidas... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398712 Tue, 23 Mar 2010 00:00:00 +0100 3398712 http://www.medworm.com/index.php?rid=3398711&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20358 The myth persists that only the labor intensive Farr radioimmunoassay and Crithidia luciliae immunofluorescence (CL-IFA) are systemic lupus erythematosus (SLE)-specific tests. We compared them to ELISA with bacteriophage [lambda] DNA (EL-dsDNA) and denatured calf thymus DNA (EL-ssDNA). By percentile ranking, the specificity cut-off level was set both out of clinical context (SOCC) on 100 blood bank donors, and in clinical context (SICC) on 100 patients with either rheumatoid arthritis or scleroderma (50/50). Clinical sensitivity was calculated on 100 random SLE patients. At 95% SICC, the sensitivity of Farr, CL-IFA, EL-dsDNA, and EL-ssDNA was similar (95%CI): 76% (66-84), 76% (66-84), 63% (53-72), and 75% (65-83), respectively; 87% of the patients were positive by at least one method and 5... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398711 Tue, 23 Mar 2010 00:00:00 +0100 3398711 http://www.medworm.com/index.php?rid=3398710&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20328 This study aimed to evaluate relationship between oxidative damage to DNA and protein glycation in patients with Type 1 diabetes. We measured urinary 8-OHdG level in diabetic patients and healthy subjects and discussed its relationship to glycated hemoglobin (HbA1c) and glycated serum protein (GSP) levels. Furthermore plasma malondialdehyde (MDA) level monitored as an important indicator of lipid peroxidation in diabetes. We studied 32 patients with Type 1 diabetes mellitus and compared the measured factors with those of 48 age-matched nondiabetic controls. GSP and MDA were measured bycolorimetric assay. Urinary 8-OHdG measurement was carried out using ELISA. In this study urinary 8-OHdG, HbA1c, plasma MDA, and GSP levels were progressively higher in diabetics than in control subjects (P (... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398710 Tue, 23 Mar 2010 00:00:00 +0100 3398710 http://www.medworm.com/index.php?rid=3398709&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20319 In this study we compared particle counts obtained by new generation automated technique, image capture analysis (IQ-200) with those of a standardized chamber counts. Design and Methods: The same 258 urine samples from different departments of a hospital assayed by IQ-200 were analyzed in parallel with the KOVA cell chamber system. Clinically significant discrepancy results (positive vs. negative) for red blood cell (RBC) and white blood cell (WBC) were also compared with those obtained by dipstick testing. Results: There was a good agreement between the automated system and sediment microscopy for RBCs, WBCs, and squamous epithelial cells (SCs) (r=0.90; r=0.80; r=0.72, respectively: P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398709 Tue, 23 Mar 2010 00:00:00 +0100 3398709 http://www.medworm.com/index.php?rid=3398708&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20301 Conclusions: Serum NOx levels and oxidative stress elevate in AA independent from the extent of the lesion. Increased NO may play a role in the increased oxidative stress in AA. J. Clin. Lab. Anal. 24:63-66, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3398708 Mon, 01 Mar 2010 00:00:00 +0100 3398708 http://www.medworm.com/index.php?rid=3188957&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20333 Early diagnosis of bacterial pneumonia plays a pivotal role in the management. We evaluated the diagnostic accuracy of procalcitonin (PCT) as compared with C-reactive protein (CRP) for the early diagnosis of bacterial pneumonia in children. In total, 92 children consisting of 46 patients of bacterial pneumonia were admitted in the Military hospital, Rawalpindi, Pakistan and equal number of controls were included. Patient's investigations were carried out at admission. PCT and CRP were analyzed on Vidas analyzer and Immulite 1000, respectively. Out of 46 pneumonia patients, 28 were male and 18 female, with a median age of 4 years. PCT levels were significantly high median (range) of 2.69 ng/ml (0.30-13.00) vs. 0.45 ng/ml (0.10-2.00) in controls. Serum CRP levels were moderately elevated wit... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188957 Wed, 20 Jan 2010 17:06:11 +0100 3188957 http://www.medworm.com/index.php?rid=3188967&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20367 The Chlamydia pneumoniae genome-encoded open reading frames Cpn0146, Cpn0147, and Cpn0308 were expressed as recombinant proteins for detecting C. pneumoniae-specific antibodies in samples from three groups of individuals including 183 with C. pneumoniae-associated respiratory infection (group I), 60 healthy blood donors (group II), and 32 with no known respiratory infection (group III). The recombinant Cpn0146 was recognized by 71 (38.8% positive recognition rate), 15 (25%) and 1 (3.1%), Cpn0147 by 75 (40.9%), 14 (23.3%), and 2 (6.3%), and Cpn0308 by 82 (44.8%), 16 (26.7%), and 0 (0%) samples from groups I, II, and III, respectively. The positive recognition rates with any of the three antigens were significantly higher in group I than those in groups II and III, suggesting that more indiv... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188967 Tue, 19 Jan 2010 00:00:00 +0100 3188967 http://www.medworm.com/index.php?rid=3188966&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20363 Conclusions: The current results suggest that platelet lipid peroxidation as marked by increased MDA level is augmented in ischemic heart diseases. The increased oxidative stress seen in these patients was accompanied by platelet activation and impaired antioxidant enzymes activity. J. Clin. Lab. Anal. 24:49-54, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188966 Tue, 19 Jan 2010 00:00:00 +0100 3188966 http://www.medworm.com/index.php?rid=3188965&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20365 This study concludes that the autoantibodies are found to be present in psoriasis patients or latent autoimmune diseases develop in psoriasis patients without any clinical symptoms. J. Clin. Lab. Anal. 24:44-48, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188965 Tue, 19 Jan 2010 00:00:00 +0100 3188965 http://www.medworm.com/index.php?rid=3188964&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20364 An agonist-induced expression of CD62P by flow cytometry analysis for evaluating platelet functional reactivity has some disadvantages. We investigated the usefulness of platelet parameters by ADVIA 120 to predict an agonist-induced expression of CD62P in stored platelets. The CD62P expression by flow cytometry and the platelet parameters by ADVIA 120 were studied in samples from 27 platelet pheresis products. Delta ([Delta]) values were calculated as the degree of change of the platelet parameters studied with or without adenosine 5[prime]-diphosphate sodium (ADP) stimulation. The CD62P expression of the ADP-activated platelets were correlated with the [Delta]platelet count (r=0.517) in the short-term storage group (within 10 hr from preparation), with the platelet component distribution ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188964 Tue, 19 Jan 2010 00:00:00 +0100 3188964 http://www.medworm.com/index.php?rid=3188963&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20355 In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P (Source: Journal of Clinical Laboratory A... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188963 Tue, 19 Jan 2010 00:00:00 +0100 3188963 http://www.medworm.com/index.php?rid=3188962&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20356 It is well known that antioxidants and reactive oxygen species play an important role in carcinogenesis. In this sudy, we attempted to evaluate antioxidant enzyme activities and lipid peroxidation levels in cancerous bladder tissue and to determine their relationship with bacterial infection. Bacterial culture was made from all urine samples using Blood and Eosin Methylene Blue agars for checking the presence of bacterial infections. We measured thiobarbituric acid reactive substances (TBARs) and activities of xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in cancerous tissues of 25 bladder cancer patients, in noncancerous adjacent bladder tissues of 13 out of these 25 patients, and in control bladder tissues of 15 patients with a non... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188962 Tue, 19 Jan 2010 00:00:00 +0100 3188962 http://www.medworm.com/index.php?rid=3188961&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20357 In conclusion, serum B-ALP, but not S-CTX, could help confirm the presence of BM in breast cancer patients. J. Clin. Lab. Anal. 24:20-24, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188961 Tue, 19 Jan 2010 00:00:00 +0100 3188961 http://www.medworm.com/index.php?rid=3188960&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20360 Authors present that serum pigment epithelium derived factor (PEDF) is an independent marker of metabolic syndrome in Caucasianpopulation. PEDF was measured with new ELISA sandwich test. J. Clin. Lab. Anal. 24:17-19, 2010. © 2010 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188960 Tue, 19 Jan 2010 00:00:00 +0100 3188960 http://www.medworm.com/index.php?rid=3188959&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20366 This study included 186 consecutive patients with EHT and no history of HF, ischemic heart disease, or atrial fibrillation. Single and multiple variable regression analyses were performed in search of clinical correlates of NT-proBNP concentrations. Results: In patients with EHT, median serum concentration of NT-proBNP was 73 pg/ml, and interquartile range (IQR) was 40-128 pg/ml. NT-proBNP was significantly higher (P (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188959 Tue, 19 Jan 2010 00:00:00 +0100 3188959 http://www.medworm.com/index.php?rid=3188958&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20361 Aim: Prolidase is a specific imidodipeptidase involved in collagen degradation. The increase in the enzyme activity is believed to be correlated with the increased intensity of collagen degradation. The study aimed to evaluate the relationship between prolidase activity and oxidative status in patients with thalassemia major. Methods: Comparison was made between 87 patients diagnosed with thalassemia major and 33 healthy children of similar age and gender. Mean age of the subjects was 7.5±4.3 years in the group of patients with thalassemia major and 8.9±3.1 years in the control group. Serum prolidase activity was measured spectrophotometrically. Oxidative status was determined using total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) measurement... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3188958 Tue, 19 Jan 2010 00:00:00 +0100 3188958 http://www.medworm.com/index.php?rid=4179073&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20426 This study demonstrated that the effect of 4°C storage is linear over a 24 hr period and independent of the initial IPF%. These observations have enabled the development of a proposed algorithm for a corrected IPF%, which is equal to the uncorrected IPF% minus 1.34 times the length of time in hours of storage at 4°C. The proposed correction algorithm extends the utility of this clinically important laboratory parameter. However, care should be taken not to extrapolate the algorithm significantly beyond 24 hr storage, because the experimental data only covered this range. J. Clin. Lab. Anal. 24:431–433, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179073 Fri, 01 Jan 2010 00:00:00 +0100 4179073 http://www.medworm.com/index.php?rid=4179072&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20424 Conclusion: In pathological urines, direct measurement of urine osmolality should be used. J. Clin. Lab. Anal. 24:426–430, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179072 Fri, 01 Jan 2010 00:00:00 +0100 4179072 http://www.medworm.com/index.php?rid=4179071&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20423 This study aims to perform gene diagnosis for Chinese family patients with Oculopharyngeal muscular dystrophy (OPMD). Genomic DNAs were extracted from the pedigrees' members. Gene diagnosis was performed for these pedigrees' members by approaches, such as DNA sequencing and GeneScan. Sequence analysis and PABPN1 genotyping showed that the mutated allele in affected members of this familyhas nine trinucleotide repeats of GCG (GCG)9, whereas the normal allele contains six trinucleotide repeats of GCG (GCG)6. The above results suggest that mutated GCG repeats in PABPN1 gene may cause OPMD in this family, and PABPN1 genotyping could be used as a convenient, highly effective, and reliable gene diagnostic test for OPMD patients. J. Clin. Lab. Anal. 24:422–425, 2010. © 2010 Wiley‐Liss, Inc. ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179071 Fri, 01 Jan 2010 00:00:00 +0100 4179071 http://www.medworm.com/index.php?rid=4179070&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20422 AbstractSerum separator tubes were introduced 35 years ago and were widely used in the clinical laboratory in China for routine collection of blood because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens. This type of tubes facilitated rapid separation of serum from cellular constituents of blood and also prevented hemolysis upon prolonged storage. However, there were some limitations associated with gel tubes (i.e., gel and analyte stability). In order to circumvent these problems, BD released a new serum separator tube containing a new gel (BD SST™ II Plus). We investigated theperformance of BD SST™ II Plus tubes for tumor marker tests using BD Serum Glass tubes as controls. Equivalence between the BD SST™ II Plus ... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179070 Fri, 01 Jan 2010 00:00:00 +0100 4179070 http://www.medworm.com/index.php?rid=4179069&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20421 AbstractSpironolactone, a potassium sparing diuretic metabolized to canrenone, is often used with digoxin to treat various conditions including congestive heart failure. Potassium canrenoate is a similar drug that is also metabolized to canrenone. Due to reported interference of spironolactone, potassium canrenoate, and their common metabolite canrenone with digoxin immunoassays, we investigated potential interference of these compounds with Dimension Vista Digoxin immunoassay using Flex reagent cartridge. Aliquots of a drug‐free serum pool were supplemented with various amounts of spironolactone, potassium canrenoate, or canrenone and apparent digoxin values were measured using Dimension Vista digoxin assay, we observed none‐detected value except when aliquots were supplemented with h... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179069 Fri, 01 Jan 2010 00:00:00 +0100 4179069 http://www.medworm.com/index.php?rid=4179068&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20420 In conclusion, we think that it is useful to evaluate IL‐6 and CRP, in combination, for the early diagnosis of neonatal sepsis. J. Clin. Lab. Anal. 24:407–412, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179068 Fri, 01 Jan 2010 00:00:00 +0100 4179068 http://www.medworm.com/index.php?rid=4179067&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20419 AbstractWe aimed to compare Hitchens–Pike–Todd–Hewitt (HPTH) enrichment broth, Todd–Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericórdia Hospital, Maringá, Paraná, Brazil, were screened by GBS using HPTH, Todd–Hewitt broth, and ABA. Twenty‐five(24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd–Hewitt broth. HPTH seems to be a high satisfa... Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179067 Fri, 01 Jan 2010 00:00:00 +0100 4179067 http://www.medworm.com/index.php?rid=4179066&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20418 Conclusion: IMA is not elevated in AOD when blood sampling is performed within 23±17 hr after symptom onset nor increases after surgery. J. Clin. Lab. Anal. 24:399–402, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179066 Fri, 01 Jan 2010 00:00:00 +0100 4179066 http://www.medworm.com/index.php?rid=4179065&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20416 Conclusions: CPB is characterised by pronounced changes in plasma sP‐s, sE‐s, TN, vWF levels, and ACE activity, which are associated with significant alteration in the intra‐ and early postoperative endothelial function observed in open heart surgery. J. Clin. Lab. Anal. 24:389–398, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179065 Fri, 01 Jan 2010 00:00:00 +0100 4179065 http://www.medworm.com/index.php?rid=4179064&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20414 Conclusions: These results indicate that platelet count and ESR are good predictors of KD. J. Clin. Lab. Anal. 24:385–388, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179064 Fri, 01 Jan 2010 00:00:00 +0100 4179064 http://www.medworm.com/index.php?rid=4179063&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20397 Conclusion: All the six tissue disorder markers are predictors of disease severity, organ failure, and outcome in non‐cardiac critically illness. Among them, Mb plays a pivotable role. The combined use of Mb and APACHE‐II suggest an effective method to determine the outcome of critical ill syndrome. J. Clin. Lab. Anal. 24:376–384, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179063 Fri, 01 Jan 2010 00:00:00 +0100 4179063 http://www.medworm.com/index.php?rid=4179062&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20386 Conclusions: This is the first study that shows the association between 5HTR2A gene polymorphisms and polysymptomatic NE. These results provide further evidence suggesting that genetic variations at 5HTR2A may influence NE treatment response. J. Clin. Lab. Anal. 24:371–375, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179062 Fri, 01 Jan 2010 00:00:00 +0100 4179062 http://www.medworm.com/index.php?rid=4179061&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20370 Conclusions: CD64 might be used either alone or combined with IL‐6 and CRP for early diagnosis of NS. The advantages of CD64 when compared with IL‐6 and CRP are rapid quantitation, very small blood volume required, and easy handling. J. Clin. Lab. Anal. 24:363–370, 2010. © 2010 Wiley‐Liss, Inc. (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=4179061 Fri, 01 Jan 2010 00:00:00 +0100 4179061 http://www.medworm.com/index.php?rid=3998680&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20415 Abstract (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3998680 Fri, 01 Jan 2010 00:00:00 +0100 3998680 http://www.medworm.com/index.php?rid=3998679&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20417 Abstract (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3998679 Fri, 01 Jan 2010 00:00:00 +0100 3998679 http://www.medworm.com/index.php?rid=3998678&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20413 Abstract (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3998678 Fri, 01 Jan 2010 00:00:00 +0100 3998678 http://www.medworm.com/index.php?rid=3998677&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20412 Abstract (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3998677 Fri, 01 Jan 2010 00:00:00 +0100 3998677 http://www.medworm.com/index.php?rid=3998676&cid=s_33643_166_f&fid=33643&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjcla.20284 Abstract (Source: Journal of Clinical Laboratory Analysis) Journal of Clinical Laboratory Analysis journals http://www.medworm.com/rss/comments.php?id=3998676 Fri, 01 Jan 2010 00:00:00 +0100 3998676