MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Experimental and Clinical Cancer Research' source. Wed, 08 Feb 2012 08:41:41 +0100 http://www.medworm.com/index.php?rid=5666834&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F11 Conclusion: Overexpression of GADD45alpha gene is due to DNA hypomethylation in ESCC. GADD45alpha may be a protective factor in DDP chemotherapy for esophageal squamous cell carcinoma. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5666834 Wed, 08 Feb 2012 05:00:00 +0100 5666834 http://www.medworm.com/index.php?rid=5659462&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F10 Conclusion: Our findings suggest that, even in the context of vulnerable MVD status and VEGF expression, overexpression of Oct-4 in tumor tissue represents a prognostic factor in primary NSCLC patients. Oct-4 may maintain NSCLC cells in a poorly differentiated state through a mechanism that depends on promoting cell proliferation. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5659462 Thu, 02 Feb 2012 05:00:00 +0100 5659462 http://www.medworm.com/index.php?rid=5647236&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F8 Conclusion: Although Ad-CALR/MAGE-A3 and Ad-CALR demonstrated antiangiogenic effects on U87 cells, the repression of invasion was significant only in Ad-CALR/MAGE-A3-treated cells. To our knowledge, this is the first description of a role for combined CALR and MAGE-A3 in the anti-invasion and antiangiogenesis of U87. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5647236 Wed, 01 Feb 2012 05:00:00 +0100 5647236 http://www.medworm.com/index.php?rid=5647235&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F9 Conclusions: The assay proved to be highly sensitive and specific, detecting even low frequencies of cytotoxic/activated cells and providing the evaluation of cytokine-expressing T cells as well as the extent of cytotoxicity against the target cells as independent functions. This assay may represent an important tool to be adopted in experimental settings including the development of vaccines or immune therapeutic strategies (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5647235 Wed, 01 Feb 2012 05:00:00 +0100 5647235 http://www.medworm.com/index.php?rid=5628189&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F7 Conclusions: The presence of some SNPs involved in DNA repair or response to oxidative stress seem to be able to predict late toxicity.Trials registration(NCT01316328). (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5628189 Tue, 24 Jan 2012 05:00:00 +0100 5628189 http://www.medworm.com/index.php?rid=5597056&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F5 Conclusions: p38 MAPK may play a role in up-regulation of hTERT, and therefore, p38 MAPK may be a useful marker in the assessment of hTERT and patients' prognosis in sarcomas. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5597056 Mon, 16 Jan 2012 05:00:00 +0100 5597056 http://www.medworm.com/index.php?rid=5597055&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F6 The purpose of this study is to determine the methylation status of Transforming growth factor-beta-inducible gene-h3 (TGFBI) and its correlation with paclitaxel chemoresistance in ovarian cancer. The methylation status of TGFBI was examined in ovarian cancer and control groups by methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP). The TGFBI expression and cell viability were compared by Quantitative Real-Time PCR, Western Blotting and MTT assay before and after demethylating agent 5-aza-2' -deoxycytidine (5-aza-dc) treatment in 6 cell lines (SKOV3, SKOV3/TR, SKOV3/DDP, A2780, 2780/TR, OVCAR8). In our results, TGFBI methylation was detected in 29/40 (72.5%) of ovarian cancer and 1/10 (10%) of benign ovarian tumors. No methylation was detected in normal ovarian tissues (P (So... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5597055 Mon, 16 Jan 2012 05:00:00 +0100 5597055 http://www.medworm.com/index.php?rid=5581145&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F4 Conclusions: This explorative analysis of SGK1 expression in NSCLC samples highlights the potential role of this factor in NSCLC patients' prognosis. Moreover, the higher expression in the squamous cell carcinoma subtype opens new therapeutic possibilities in this NSCLC subtype by designing specific kinase inhibitors. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5581145 Thu, 12 Jan 2012 05:00:00 +0100 5581145 http://www.medworm.com/index.php?rid=5581146&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F3 Conclusions: These findings provide new evidence that tumor-derived TGF-beta1 inhibits migration of DCs from tumors to their draining lymph nodes, and this immunosuppressive effect of TGF-beta1 increases the likelihood of metastasis in the affected nodes. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5581146 Tue, 10 Jan 2012 05:00:00 +0100 5581146 http://www.medworm.com/index.php?rid=5572010&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F2 This study aims to investigate the in vitro effects of Ulinastatin (UTI) and Taxotere (TXT) on cell proliferation; cell apoptosis; xenografted tumor growth; and expression of insulin-like growth factor receptor 1 (IGF-1R), platelet-derived growth factor A (PDGFA), nerve growth factor (NGF), c-Jun N-terminal kinase 2 (JNk-2), and NF-kappaB in a human primary breast cancer cells and breast cancer cell line MDA-MB-231. Methods: The cell lines cultured were divided into four groups: 1) control group, 2) UTI group, 3) TXT group, and 4) UTI+TXT group. The method of MTT essay, flow cytometry, and RT-PCR were used to detect cell proliferation, cell apoptosis, and expression of IGF-1R, PDGFA, NGF, NF-kappaB, JNk-2, respectively. The growth of xenografted tumor in nude mice was used to calculate the... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5572010 Thu, 05 Jan 2012 05:00:00 +0100 5572010 http://www.medworm.com/index.php?rid=5559595&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F31%2F1%2F1 This study involved 12 normal cervical squamous epithelia, 48 cervical intraepithelial neoplasia (CIN), and 68 cervical cancer. The expression of TFPI-2, Ki-67 and vascular endothelial growth factor (VEGF) were investigated by immunohistochemistry staining. The apoptolic index(AI) was determined with an in situ end-labeling assay(TUNEL). And the marker of CD34 staining was used as an indicator of microvessel density (MVD).Results TFPI-2 expression has a decreasing trend with the progression of cervical cancer and was significantly correlated with FIGO stage, lymph node metastasis and HPV infection. In addition, there were significant positive correlations between the grading of TFPI-2 expression and AI(P=0.004). In contrastthe expression of TFPI-2 and VEGF or MVD was negatively correlated(... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5559595 Mon, 02 Jan 2012 05:00:00 +0100 5559595 http://www.medworm.com/index.php?rid=5552156&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F115 Adjuvant chemotherapy for non-small-cell lung carcinoma (NSCLC) is a debated issue in clinical oncology. Although it is considered a standard for resected stage II-IIIA patients according to the available guidelines, many questions are still open. Among them, it should be acknowledged that the treatment for stage IB disease has shown so far a limited (if sizable) efficacy, the role of modern radiotherapies requires to be evaluated in large prospective randomized trials and the relative impact of age and comorbidities should be weighted to assess the reliability of the trials' evidences in the context of the everyday-practice. In addition, a conclusive evidence of the best partner for cisplatin is currently awaited as well as a deeper investigation of the fading effect of chemotherapy over ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5552156 Thu, 29 Dec 2011 05:00:00 +0100 5552156 http://www.medworm.com/index.php?rid=5552155&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F116 Background: Whether Folic acid is a potential drug that may prevent the progression of colorectal carcinoma and when to use are important healthy issues we focus on. Our study is to examine the effect of folic acid on the development of the CRC and the optimal time folic acid should be provided in a mouse-ICR model induced by 1, 2-Dimethylhydrazine. Also, we investigated the gene expression profile of this model related to folic acid.MethodFemale ICR mouse (n=130) were divided into 7 groups either with the treatment of 1, 2-Dimethylhydrazine (20mg/kg bodyweight) weekly or folic acid (8mg/kg bodyweight) twice a week for 12 or 24weeks. Using a 4x44 K Agilent whole genome oligo microarray assay, different gene expression among groups (NS, DMH, FA2FA3) were identified and selected genes were v... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5552155 Thu, 29 Dec 2011 05:00:00 +0100 5552155 http://www.medworm.com/index.php?rid=5552154&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F117 Conclusions: The incidence of nodal metastases was approximately 5% in undifferentiated type mucosal (pT1a) tumors, and higher in submucosal (pT1b) tumors. The sensitivity of preoperative diagnosis of nodal metastases in EGC using computed tomography was relatively low in this study. Therefore at present surgery with adequate lymphadenectomy should be performed as curative treatment for undifferentiated type EGC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5552154 Thu, 29 Dec 2011 05:00:00 +0100 5552154 http://www.medworm.com/index.php?rid=5524226&cid=s_37196_6_f&fid=37196&url=%24%7Bitem.link%7D Conclusions: Our results indicate that up-regulation of MEIS1 is important for sustaining proliferation of leukemic cells and that down-regulation of MEIS1 or up-regulation of PREP1 and PBX genes could be implicated in the modulation of the cellular response to chemotherapeutic-induced apoptosis. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5524226 Tue, 20 Dec 2011 05:00:00 +0100 5524226 http://www.medworm.com/index.php?rid=5524225&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F113 Conclusion: We conclude that CLU could be a potential molecular target to predict survival while targeting this s-CLU may improve survival of patients with ovarian cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5524225 Tue, 20 Dec 2011 05:00:00 +0100 5524225 http://www.medworm.com/index.php?rid=5524224&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F114 Conclusions: The results of our study demonstrate that the Zfx gene is highly expressed in glioma tissue and in glioma cell lines. Furthermore, Zfx may play a critical role in cell proliferation, cell cycle distribution, and apoptosis of human malignant glioma cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5524224 Tue, 20 Dec 2011 05:00:00 +0100 5524224 http://www.medworm.com/index.php?rid=5476247&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F111 Conclusions: When using body fluids for EGFR mutation analysis, positive result is consistently a good indicator for TKIs therapy, the predictive effect was no less than that of tumor tissue, no matter what method was employed. However, even been reclassified by ARMS, the correlation between negative results and clinical outcome of TKIs was still unsatisfied. The results indicate that false negative mutation still existed, which may be settled by using method with sensitivity to single DNA molecule or by optimizing the extraction procedure with RNA or CTC to ensure adequate amount of tumor-derived nucleic acid for the test. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5476247 Tue, 06 Dec 2011 05:00:00 +0100 5476247 http://www.medworm.com/index.php?rid=5468532&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F110 Conclusion: These data suggest that miR-15a/16-1 may function as a tumor suppressor to regulate leukemic cell proliferation potentially by down-regulating the WT1 oncogene. However WT1 is not directly targeted by miR-15a/16-1 through miRNA-mRNA base pairing, therefore more study are required to understand the mechanism by which miR-15a/16-1 downregulate WT1. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5468532 Thu, 01 Dec 2011 05:00:00 +0100 5468532 http://www.medworm.com/index.php?rid=5407351&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F108 Conclusions: Our data show that p16INK4a and pAkt are over-expressed in BCC and that the high expression of p16INK4a and of Akt2 isoform is often associated with the presence of beta-HPV species 2 (i.e. HPV 15). The association of these viruses with the up-regulation of p16INK4a and Akt/PI3K pathway suggests that in a subtype of BCC these viruses may exert a role in the carcinogenesis or in other, still undefined, biological property of these tumors. If this particular type of BCC reflects a different biology it will remain undisclosed until further studies on a larger number of samples will be performed. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5407351 Mon, 14 Nov 2011 05:00:00 +0100 5407351 http://www.medworm.com/index.php?rid=5400358&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F107 Conclusions: In gastric cancer, adiponectin has the possibility to be involved in cell growth suppression via AdipoR1. The presence of AdipoR1 could be a novel anticancer therapeutic target in gastric cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5400358 Fri, 11 Nov 2011 05:00:00 +0100 5400358 http://www.medworm.com/index.php?rid=5400360&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F105 Conclusion A peptide ZT-2, which binds specifically to the renal carcinoma cell line A498 was selected from phage display peptide libraries. Therefore, it provides a potential tool for early diagnosis of renal carcinoma or targeted drug delivery in chemotherapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5400360 Thu, 10 Nov 2011 05:00:00 +0100 5400360 http://www.medworm.com/index.php?rid=5400359&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F106 Background: We evaluated the expression of human trophoblastic cell-surface marker (Trop-2) and the potential of hRS7 - a humanized monoclonal anti-Trop-2 antibody - as a therapeutic strategy against treatment-refractory human uterine (UMMT) and ovarian (OMMT) carcinosarcoma cell lines.Materials and MethodsTrop-2 expression was evaluated by immunohistochemistry (IHC) in paraffin-embedded tumor tissues, by real-time polymerase-chain-reaction (RT-PCR) and flow-cytometry in cell lines. Sensitivity to hRS7 antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity was tested using 5-hour chromium-release assays against UMMT and OMMT cells. Results: Trop-2 expression was elevated in 9 of 26 (35%) UMMT and 8 of 14 (57%) OMMT tissues tested by IHC. Positivity for Trop-2... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5400359 Thu, 10 Nov 2011 05:00:00 +0100 5400359 http://www.medworm.com/index.php?rid=5360059&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F103 This study focused on whether CKI could inhibit MCF-7 SP cells in vitro and in vivo.Methods The analysis of CKI on SP population and the main genes of Wnt signaling pathway were studied first. Then we studied the tumorigenicity of SP cells and the effects of CKI on SP cells in vivo. The mice inoculated with 10,000 SP cells were randomly divided into three groups (6 in each group) and treated with CKI, cisplatin and saline (as a control) respectively for 7 weeks. The tumor formation rates of each group were compared. The main genes and proteins of the Wnt signaling pathway were analyzed by RT-PCR and western blot.Results CKI suppressed the size of SP population (approximately 90%), and down-regulated the main genes of Wnt signaling pathway. We also determined that MCF-7 SP cells were more t... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5360059 Fri, 28 Oct 2011 04:00:00 +0100 5360059 http://www.medworm.com/index.php?rid=5360058&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F104 Conclusions: Our results demonstrated that ultrasound microbubble-mediated delivery of MDR1 siRNA was safe and effective in L2-RYC cells. MDR1 silencing led to decreased P-glycoprotein activity and drug resistance of L2-RYC cells, which may be explored as a novel approach of combined gene and chemotherapy for yolk sac carcinoma. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5360058 Fri, 28 Oct 2011 04:00:00 +0100 5360058 http://www.medworm.com/index.php?rid=5347024&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F102 Conclusions: The results presented here indicate for the first time that salivary alpha-amylase affects cell growth in rat mammary epithelial cells and in breast tumor cells of human origin. Thus, alpha-amylase may be considered a novel, promising target for balancing cellular growth, which may provide an interesting tool for tumor prophylaxis and treatment. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5347024 Tue, 25 Oct 2011 04:00:00 +0100 5347024 http://www.medworm.com/index.php?rid=5347025&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F101 Conclusions: We didn't reveal any important correlations between clinicopathological findings and galectin-3 and cyclin D1 expression in non small cell lung cancer. We didn't observed also prognostic value of cyclin D1 or galectin-3 expression. But we showed higher cyclin D1 expression in galectin-3 negative tumor tissues. We revealed also differences in correlations between galectin-3 and cyclin D1 expression in two main histopathological types of NSCLC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5347025 Mon, 24 Oct 2011 04:00:00 +0100 5347025 http://www.medworm.com/index.php?rid=5335265&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F100 Conclusions: Finding that MMR protein levels can be measured in fresh lymphocytes, and given that cells with heterozygote MMR mutations have reduced levels of full-length MMR proteins, suggests that our immunoassay could be advanced to a quantitative test for screening populations at high risk for LS. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5335265 Fri, 21 Oct 2011 04:00:00 +0100 5335265 http://www.medworm.com/index.php?rid=5335266&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F99 Conclusions: This meta-analysis suggests that the MspI and exon 7 polymorphisms of CYP1A1 correlate with increased lung cancer susceptibility and there is an interaction between two genotypes of CYP1A1 polymorphism and smoking, but these associations vary in different ethnic populations, histological types of lung caner and gender of case and control population. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5335266 Thu, 20 Oct 2011 04:00:00 +0100 5335266 http://www.medworm.com/index.php?rid=5323791&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F98 DNA methylation has been widely used in classification, early diagnosis, therapy and prediction of metastasis as well as recurrence of cervical cancer. DNMT methyltransferase 1 (DNMT1), which plays a significant role in maintaining DNA methylation status and regulating the expression of tumor suppressor genes. The aim of this research was to investigate the relationship between DNMT1 and abnormal methylation of tumor suppressor genes and malignant phenotype in cervical cancer.Levels of DNMT1 mRNA and protein were detected using qPCR and Western blot, respectively. Cell proliferation was analyzed by MTT and apoptosis was performed by Annexin V-FITC/PI double staining flow cytometry, respectively. MeDIP-qPCR and qPCR were performed to measure demethylation status and mRNA re-expression level... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5323791 Mon, 17 Oct 2011 04:00:00 +0100 5323791 http://www.medworm.com/index.php?rid=5314059&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F97 Conclusions: These findings suggested that BDNF/TrkB are essential for HCC cells survival and invasion. BDNF/TrkB signaling should probably be an effective target to prevent HCC advancement. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5314059 Fri, 14 Oct 2011 04:00:00 +0100 5314059 http://www.medworm.com/index.php?rid=5314060&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F96 In this study we have investigated alternative activation of beta-catenin by HGF/c-Met signaling in a large cohort of 84 HB patients enrolled in the SIOPEL-3 clinical trial. Methods: We performed immunohistochemistry, using antibodies to total beta-catenin and tyrosine654-phosphorylated beta-catenin, which is a good surrogate marker of HGF/c-Met activation. CTNNB1 mutation analysis was also carried out on all samples. We also investigated beta-catenin pathway activation in two liver cancer cell lines, HuH-6 and HuH-7. Results: Aberrant beta-catenin expression was seen in the cytoplasm and/or nucleus of 87% of tumour samples. Our results also revealed a large subset of HB, 83%, with cytoplasmic expression of tyrosine654-phosphorylated beta-catenin and 30% showing additional nuclear accumula... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5314060 Wed, 12 Oct 2011 04:00:00 +0100 5314060 http://www.medworm.com/index.php?rid=5304140&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F95 Increased polyamine concentrations in the blood and urine of cancer patients reflect the enhanced levels of polyamine synthesis in cancer tissues arising from increased activity of enzymes responsible for polyamine synthesis. In addition to their de novo polyamine synthesis, cells can take up polyamines from extracellular sources, such as cancer tissues, food, and intestinal microbiota. Because polyamines are indispensable for cell growth, increased polyamine availability enhances cell growth. However, the malignant potential of cancer is determined by its capability to invade to surrounding tissues and metastasize to distant organs. The mechanisms by which increased polyamine levels enhance the malignant potential of cancer cells and decrease anti-tumor immunity are reviewed. Cancer cells... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5304140 Tue, 11 Oct 2011 04:00:00 +0100 5304140 http://www.medworm.com/index.php?rid=5285249&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F93 Conclusions: The FDA's adverse event reporting system, AERS, and the data mining methods used herein are useful for confirming drug-associated adverse events, but the number of co-occurrences is an important factor in signal detection. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5285249 Wed, 05 Oct 2011 04:00:00 +0100 5285249 http://www.medworm.com/index.php?rid=5285248&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F94 Conclusions: The long-term survival after treatment with a definitive 5-FU/CDDP-based CRT possibly depends on the plasma concentrations of 5-FU, and further clinical studies with a larger number of cases are needed to clarify the relationship between them. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5285248 Wed, 05 Oct 2011 04:00:00 +0100 5285248 http://www.medworm.com/index.php?rid=5285251&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F91 Breast and ovarian cancers are among the 10 leading cancer types in females with mortalities of 15% and 6%, respectively. Despite tremendous efforts to conquer malignant diseases, the war on cancer declared by Richard Nixon four decades ago seems to be lost. Approximately 21,800 women in the US will be diagnosed with ovarian cancer in 2011. Therefore, its incidence is relatively low compared to breast cancer with 207.090 prognosed cases in 2011. However, overall survival unmasks ovarian cancer as the most deadly gynecological neoplasia. Platinum-based chemotherapy is emerging as an upcoming treatment modality especially in triple negative breast cancer. However, in ovarian cancer Platinum-complexes for a long time are established as first line treatment. Emergence of a resistant phenotype ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5285251 Tue, 04 Oct 2011 04:00:00 +0100 5285251 http://www.medworm.com/index.php?rid=5285250&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F92 Conclusions: Our findings indicate that MTX-mediated synchronization of target cells allowed a significant improvement of retroviral HSV-tk gene transfer, resulting in an increased cell apoptosis in response to GCV. Pharmacological control of cell cycle may thus be a useful strategy to optimize the efficiency of retroviral-mediated cancer gene therapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5285250 Tue, 04 Oct 2011 04:00:00 +0100 5285250 http://www.medworm.com/index.php?rid=5285252&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F90 Previous data demonstrate that JMJD2A is a cancer-associated gene and may be involved in human breast cancer by demethylation of H3K9me3. The aim of this study was to investigate depressive effects on JMJD2A by transfection with JMJD2A-sepcific siRNA in human breast cancer cell line MDA-MB-231 and effects on cell proliferation, invasion and migration. JMJD2A-specific siRNA was chemically synthesised and transfected into human breast cancer cell line MDA-MB-231. Expression levels of JMJD2A were detected by quantitative real-time PCR and Western Blot analysis. Cells proliferation was evaluated by using flow cytometric anlysis and MTT assay. The abilities of invasion and migration were evaluated by cell migration and invasion assay with Boyden chambers. The results showed that the transfectio... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5285252 Mon, 03 Oct 2011 04:00:00 +0100 5285252 http://www.medworm.com/index.php?rid=5273364&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F89 Conclusions: Our data indicate that changes in the cellular level of galectin-3 correlate with the development of clear cell renal cell carcinoma, which is in line with previously published data on this specific type of tumor. In most of these studies the lectin tends to be highly expressed in tumor tissues. Furthermore, this study suggests that the increase in the proportion of galectin-3 affects the balance from a cytosolic distribution towards translocation into the nucleus. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5273364 Thu, 29 Sep 2011 04:00:00 +0100 5273364 http://www.medworm.com/index.php?rid=5260263&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F88 This study aimed to investigate the functions of PKCepsilon in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCepsilon was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCepsilon by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCepsilon siRNA-transfected cells. These results indicate that the overexpression of PKCepsilon is associated with an aggressive phenotype of clea... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5260263 Wed, 28 Sep 2011 04:00:00 +0100 5260263 http://www.medworm.com/index.php?rid=5260264&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F87 Apoptosis is an ordered and orchestrated cellular process that occurs in physiological and pathological conditions. It is also one of the most studied topics among cell biologists. An understanding of the underlying mechanism of apoptosis is important as it plays a pivotal role in the pathogenesis of many diseases. In some, the problem is due to too much apoptosis, such as in the case of degenerative diseases while in others, too little apoptosis is the culprit. Cancer is one of the scenarios where too little apoptosis occurs, resulting in malignant cells that will not die. The mechanism of apoptosis is complex and involves many pathways. Defects can occur at any point along these pathways, leading to malignant transformation of the affected cells, tumour metastasis and resistance to antic... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5260264 Mon, 26 Sep 2011 04:00:00 +0100 5260264 http://www.medworm.com/index.php?rid=5260265&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F86 Conclusions Our findings showed that AQP3 might positively regulate MMPs proteins expression through PI3K/AKT signal pathway in human gastric carcinoma SGC-7901 cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5260265 Sun, 25 Sep 2011 04:00:00 +0100 5260265 http://www.medworm.com/index.php?rid=5250409&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F85 Conclusion: These results suggest that different patterns of NF-B and Notch1 signaling contribute to lymph nodes metastasis and tumor-induced lymphangiogenesis of ESCC, and reveal that up-regulation of NF-B is associated with down-regulation of Notch1 in tumor tissue. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5250409 Thu, 22 Sep 2011 04:00:00 +0100 5250409 http://www.medworm.com/index.php?rid=5236886&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F84 Conclusions: We conclude that TLR9 expression is an independent prognostic marker of RCC and the absence of TLR9 expression is related to poorer prognosis in RCC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5236886 Mon, 19 Sep 2011 04:00:00 +0100 5236886 http://www.medworm.com/index.php?rid=5225006&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F83 Conclusions: RNA interference (RNAi) against MACC1 could serve as a promising intervention strategy for gene therapy of ovarian carcinoma, and the antitumor effects of MACC1 knockdown might involve in the inhibition of HGF/Met and MEK/ERK pathways. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5225006 Fri, 16 Sep 2011 04:00:00 +0100 5225006 http://www.medworm.com/index.php?rid=5225007&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F82 Conclusions: These results suggest that IDO helps to create a tolerogenic milieu in breast tumors by directly inducing T cell apoptosis and enhancing Treg-mediated immunosuppression. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5225007 Wed, 14 Sep 2011 04:00:00 +0100 5225007 http://www.medworm.com/index.php?rid=5208114&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F80 Glioblastoma multiforme (GBM) carries a dismal prognosis primarily due to its aggressive proliferation in the brain regulated by complex molecular mechanisms. One promising molecular target in GBM is over-expressed basic fibroblast growth factor (bFGF), which has been correlated with growth, progression, and vascularity of human malignant gliomas. Previously, we reported significant antitumor effects of an adenovirus-vector carrying bFGF small interfering RNA (Ad-bFGF-siRNA) in glioma in vivo and in vitro. However, its mechanisms are unknown. Signal transducer and activator of transcription 3 (STAT3) is constitutively active in GBM and correlates positively with the glioma grades. In addition, as a specific transcription factor, STAT3 serves as the convergent point of various signaling pat... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5208114 Fri, 09 Sep 2011 04:00:00 +0100 5208114 http://www.medworm.com/index.php?rid=5175542&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F79 Conclusions: Although UCH-L1 appears to be involved in carcinogenic processes in NSCLC cell lines, the absence of correlation with patient survival indicates that caution is required in the use of UCH-L1 as a potential prognostic marker for advanced stage and metastasis in lung carcinoma. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5175542 Mon, 29 Aug 2011 23:00:00 +0100 5175542 http://www.medworm.com/index.php?rid=5161218&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F78 Conclusions: These data indicate that frequency of these T cell subsets in lymph nodes may not be a useful tool for predicting patient outcome. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5161218 Tue, 23 Aug 2011 23:00:00 +0100 5161218 http://www.medworm.com/index.php?rid=5131812&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F76 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5131812 Sun, 14 Aug 2011 23:00:00 +0100 5131812 http://www.medworm.com/index.php?rid=5131811&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F77 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5131811 Sun, 14 Aug 2011 23:00:00 +0100 5131811 http://www.medworm.com/index.php?rid=5126134&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F75 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5126134 Thu, 11 Aug 2011 23:00:00 +0100 5126134 http://www.medworm.com/index.php?rid=5109087&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F74 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5109087 Tue, 09 Aug 2011 23:00:00 +0100 5109087 http://www.medworm.com/index.php?rid=5109088&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F73 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5109088 Sun, 07 Aug 2011 23:00:00 +0100 5109088 http://www.medworm.com/index.php?rid=5097238&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F72 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5097238 Wed, 03 Aug 2011 23:00:00 +0100 5097238 http://www.medworm.com/index.php?rid=5078694&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F71 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5078694 Thu, 28 Jul 2011 23:00:00 +0100 5078694 http://www.medworm.com/index.php?rid=5070067&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F70 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5070067 Tue, 26 Jul 2011 23:00:00 +0100 5070067 http://www.medworm.com/index.php?rid=5052459&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F69 ${item.shortDescription} (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5052459 Wed, 20 Jul 2011 23:00:00 +0100 5052459 http://www.medworm.com/index.php?rid=5030180&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F68 Conclusions, these results suggest that MDR1 C3435T polymorphism might play a role in HL occurrence; however this polymorphism is not correlated with the clinical response to ABVD. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5030180 Fri, 15 Jul 2011 23:00:00 +0100 5030180 http://www.medworm.com/index.php?rid=5009659&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F67 Background: Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms. Methods: Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [3H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays. Results: Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=5009659 Wed, 06 Jul 2011 23:00:00 +0100 5009659 http://www.medworm.com/index.php?rid=4977443&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F66 Conclusion: Oxymatrine can induce apoptotic cell death of human pancreatic cancer, which might be attributed to the regulation of Bcl-2 and IAP families, release of mitochondrial cytochrome c and activation of caspase-3. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4977443 Tue, 28 Jun 2011 23:00:00 +0100 4977443 http://www.medworm.com/index.php?rid=4936525&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F65 Conclusion: Despite the small sample size, the results on the immune response, safety and tolerability, combined with the results of other studies, are encouraging to the conduction of a large clinical trial with multiples doses in patients with early lung cancer who underwent surgical treatment.Trial Registration - Current Controlled Trials ISRCTN45563569 (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4936525 Thu, 16 Jun 2011 23:00:00 +0100 4936525 http://www.medworm.com/index.php?rid=4894849&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F64 Background: Approximately 20% of children and adolescents in Europe are overweight. Survivors of pediatric acute lymphoblastic leukemia (ALL) are at increased risk of overweight and obesity. The purpose of this study was to assess leptin and leptin soluble receptor levels, as well as polymorphisms of selected genes in survivors of pediatric ALL, and the influence of chemo- and radiotherapy on development of overweight in the context of leptin regulation. Methods: Eighty two patients (55% males), of median age 13.2 years (m: 4.8 years; M: 26.2 years) were included in the study. The ALL therapy was conducted according to modified Berlin-Frankfurt-Munster (BFM; n=69) regimen or New York (n=13) regimen. In 38% of patients cranial radiotherapy (CRT) was used in median dose of 18.2Gy (m: 14Gy; M... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4894849 Tue, 31 May 2011 23:00:00 +0100 4894849 http://www.medworm.com/index.php?rid=4842658&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F62 Conclusions: Our results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer. The data also suggested that TAMs may involve in tumor immunosuppression through overexpressed IL-10. Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4842658 Thu, 19 May 2011 23:00:00 +0100 4842658 http://www.medworm.com/index.php?rid=4842660&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F60 Conclusions: ShRNA targeted against MTA1 could specifically mediate the MTA1 gene silencing and consequentially recover the protein expression of ER alpha, resulting in increase sensitivity of antiestrogens, as well as suppress the protein levels of MMP-9 and cyclinD1 in ER-negative human breast cancer cell lines MDA-MB-231. Silencing effect of MTA1 could efficiently inhibit the invasion and proliferation in MDA-MB-231 cells.The shRNA interference targeted against MTA1 may have potential therapeutic utility in human breast cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4842660 Wed, 18 May 2011 23:00:00 +0100 4842660 http://www.medworm.com/index.php?rid=4842659&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F61 Hypoxia inducible factor-1 (HIF-1) has been considered as a critical transcriptional factor in response to hypoxia. It can increase P-glycoprotein (P-Gp) thus generating the resistant effect to chemotherapy. At present, the mechanism regulating HIF-1 alpha is still not fully clear in hypoxic tumor cells. Intracellular redox status is closely correlated with hypoxic micro-environment, so we investigate whether alterations in the cellular redox status lead to the changes of HIF-1 alpha expression. HepG2 cells were exposed to Buthionine sulphoximine (BSO) for 12h prior to hypoxia treatment. The level of HIF-1 alpha expression was measured by Western blot and immunocytochemistry assays. Reduce glutathione (GSH) concentrations in hypoxic cells were determined using glutathione reductase/5,5'-di... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4842659 Wed, 18 May 2011 23:00:00 +0100 4842659 http://www.medworm.com/index.php?rid=4830880&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F58 Oncogene addiction is a phenomenon that the survival of cancer cells depends on an activated oncogene or inactivation of tumor suppressor gene, and is regarded as the 'Achilles heel' of the successful molecular targeted therapies in cancer. However, the role of oncogene addiction in gliomas has not been elucidated systematically. In this review, we summarize the current experimental and clinical evidence for the concept of oncogene addiction and describe the mechanisms explaining oncogene addiction in gliomas. And the clinical implications for oncogene addiction in molecular targeted therapy are further emphasized. In addition, we discuss future direction for defining complex "oncogene addiction network" through the integrated analysis of multiple platforms in the flow of genetic informati... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4830880 Mon, 16 May 2011 23:00:00 +0100 4830880 http://www.medworm.com/index.php?rid=4830879&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F59 Conclusions: Our results show that human serum extracts enriched with endogenous long-chain hydroxy fatty acids possess anti-inflammatory and anti-proliferative activity. These findings support a hypothesis that the reduction of these metabolites with age may result in a compromised ability to defend against uncontrolled cell growth and inflammation, and could therefore represent a significant risk for the development of CRC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4830879 Mon, 16 May 2011 23:00:00 +0100 4830879 http://www.medworm.com/index.php?rid=4830882&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F56 Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics. Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated wit... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4830882 Sun, 15 May 2011 23:00:00 +0100 4830882 http://www.medworm.com/index.php?rid=4830881&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F57 Conclusions: pyrosequencing is then a highly accurate method for detecting delta LRE and L858R EGFR mutations in patients with NSCLC when the samples contain at least 20% of tumor cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4830881 Sun, 15 May 2011 23:00:00 +0100 4830881 http://www.medworm.com/index.php?rid=4821705&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F55 Conclusions: MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4821705 Thu, 12 May 2011 23:00:00 +0100 4821705 http://www.medworm.com/index.php?rid=4813963&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F54 Background: Although the addition of bevacizumab significantly improves the efficacy of chemotherapy for advanced breast cancer, regulatory concerns still exist with regard to the magnitude of the benefits and the overall safety profile. Methods: A literature-based meta-analysis to quantify the magnitude of benefit and safety of adding bevacizumab to chemotherapy for advanced breast cancer patients was conducted. Meta-regression and sensitivity analyses were also performed to identify additional predictors of outcome and to assess the influence of trial design. Results: Five trials (3,841 patients) were gathered. A significant interaction according to treatment line was found for progression-free survival (PFS, p=0.027); PFS was significantly improved for 1st line (Hazard Ratio, HR 0.68, p... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4813963 Wed, 11 May 2011 23:00:00 +0100 4813963 http://www.medworm.com/index.php?rid=4805618&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F53 After publication of this work [Guo-Qing P et al. J Exp Clin Cancer Res. 2010; 29(1): 129], we noted that we inadvertently made an error order of author affiliations. The corrected order of author affiliations was listed as above. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4805618 Tue, 10 May 2011 23:00:00 +0100 4805618 http://www.medworm.com/index.php?rid=4805619&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F52 Background: An advantage of the Intensity Modulated Radiotherapy (IMRT) technique is the feasibility to deliver different therapeutic dose levels to PTVs in a single treatment session using the Simultaneous Integrated Boost (SIB) technique. The paper aims to describe an automated tool to calculate the dose to be delivered with the SIB-IMRT technique in different anatomical regions that have the same Biological Equivalent Dose (BED), i.e. IsoBED, compared to the standard fractionation. Methods: Based on the Linear Quadratic Model (LQM), we developed software that allows treatment schedules, biologically equivalent to standard fractionations, to be calculated. The main radiobiological parameters from literature are included in a database inside the software, which can be updated according to... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4805619 Sun, 08 May 2011 23:00:00 +0100 4805619 http://www.medworm.com/index.php?rid=4795203&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F51 Conclusions: High CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination as well as with tumor cell migration and invasion in vitro. Its underlying mechanism probably involves the PI3K/Akt signal pathway. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4795203 Fri, 06 May 2011 23:00:00 +0100 4795203 http://www.medworm.com/index.php?rid=4790166&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F50 Maintenance therapy is emerging as a treatment strategy in the management of advanced non small cell lung cancer (NSCLC). Initial trials addressing the question of duration of combination chemotherapy failed to show any overall survival benefit for the prolonged administration over a fixed number of cycles and maintenance treatment was associated with an increased risk for cumulative toxicity. Nowadays several agents with different ways of administration and a different pattern of toxicity have been formally investigated in the maintenance setting. Maintenance strategies include continuing with an agent already present in the induction regimen or switching to a different one. Taking into consideration that no comparative trials of maintenance with different chemotherapy drugs or targeted a... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4790166 Thu, 05 May 2011 23:00:00 +0100 4790166 http://www.medworm.com/index.php?rid=4782156&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F49 Conclusions: Administration of MSCs in chemically induced HCC has tumor suppressive effects as evidenced by down regulation of Wnt signaling target genes concerned with antiapoptosis, mitogenesis, cell proliferation and cell cycle regulation, with subsequent amelioration of liver histopathological picture and liver function. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4782156 Wed, 04 May 2011 23:00:00 +0100 4782156 http://www.medworm.com/index.php?rid=4776205&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F48 Conclusions: Our data indicated that treatment with CpG-ODN inhibited the HepG2 cells-mediated Jurkat cell apoptosis by modulating the Fas/FasL pathway. Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4776205 Mon, 02 May 2011 23:00:00 +0100 4776205 http://www.medworm.com/index.php?rid=4771796&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F47 Overwhelming evidence from leukemia research has shown that the clonal population of neoplastic cells exhibits marked heterogeneity with respect to proliferation and differentiation. There are rare stem cells within the leukemic population that possess extensive proliferation and self-renewal capacity not found in the majority of the leukemic cells. These leukemic stem cells are necessary and sufficient to maintain the leukemia.While the hematopoietic stem cell (HSC) origin of CML was first suggested over 30 years ago, recently CML-initiating cells beyond HSCs are also being investigated. We have previously isolated fetal liver kinase-1-positive (Flk1) cells carrying the BCR/ABL fusion gene from the bone marrow of Philadelphia chromosome-positive (Ph+) patients with hemangioblast property.... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4771796 Sun, 01 May 2011 23:00:00 +0100 4771796 http://www.medworm.com/index.php?rid=4763536&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F46 Conclusions: VM existed in gallbladder carcinomas by both three-dimensional matrix of highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells in vitro and GBC-SD nude mouse xenografts in vivo. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4763536 Thu, 28 Apr 2011 23:00:00 +0100 4763536 http://www.medworm.com/index.php?rid=4754811&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F45 Conclusions: Our results suggest that an imbalance in tumour progenitor subpopulations imbalances the functional relationship between proliferation and senescence, creating a microenvironment favouring tumour progression. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4754811 Mon, 25 Apr 2011 23:00:00 +0100 4754811 http://www.medworm.com/index.php?rid=4725360&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F44 Conclusions: Silibinin induces apoptotic cell death through a calpain-dependent mechanism involving PKC, ROS, and AIF nuclear translocation in U87MG human glioma cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4725360 Mon, 18 Apr 2011 23:00:00 +0100 4725360 http://www.medworm.com/index.php?rid=4725361&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F43 Background: To conserve laryngeal function and elevate living quality of laryngeal squamous cell carcinoma (LSCC) patients, we designed antisense oligodeoxynucleotides (AS-ODNs) to reduce expression of ATM and to enhance the apoptosis of hep-2 cells to radiation in vitro and in vivo. Methods: The expression of ATM mRNA and protein in hep-2 cells were examined by real-time quantitative PCR and western blotting respectively. Clonogenic survival assay was carried out to detect the survival ability of hep-2 cells after irradiation, and analyzed the cell apoptosis by flow cytometry. The volume of solid tumors was measured, while TUNEL assay and western blotting used to analyze cell apoptosis and protein expression after irradiation. Results: The relative ATM mRNA and protein expression in hep-2... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4725361 Sat, 16 Apr 2011 23:00:00 +0100 4725361 http://www.medworm.com/index.php?rid=4715294&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F41 Over-expressed in numerous cancers, Ubiquitin-like containing PHD Ring Finger 1 (UHRF1, also known as ICBP90 or Np95) is characterized by a SRA domain (Set and Ring Associated) which is found only in the UHRF family. UHRF1 constitutes a complex with histone deacetylase 1 (HDAC1) and DNA methyltransferase 1 (DNMT1) via its SRA domain and represses the expression of several tumour suppressor genes (TSGs) including p16INK4A, hMLH1, BRCA1 and RB1. Conversely, UHRF1 is regulated by other TSGs such as p53 and p73. UHRF1 is hypothetically involved in a macro-molecular protein complex called "ECREM" for "Epigenetic Code Replication Machinery". This complex would be able to duplicate the epigenetic code by acting at the DNA replication fork and by activating the right enzymatic activity at the righ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4715294 Thu, 14 Apr 2011 23:00:00 +0100 4715294 http://www.medworm.com/index.php?rid=4709989&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F40 Conclusions: Our preliminary results suggest that small cutaneous KS lesions - in both CKS and AIDS-KS patients- display similar B-mode ultrasound patterns ( hypoechoic, well defined, superficial lesions). However, the color Doppler signal, which is associated with endothelial activity and angiogenesis, which play a substantial role in KS progression, could constitute a useful tool for evaluating disease activity. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4709989 Tue, 12 Apr 2011 23:00:00 +0100 4709989 http://www.medworm.com/index.php?rid=4698125&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F39 Conclusions: Both anthracycline- containing regimens evaluated in the present study seem to be active and with a satisfactory tolerability in anthracycline-naive relapsed breast cancer patients. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4698125 Mon, 11 Apr 2011 23:00:00 +0100 4698125 http://www.medworm.com/index.php?rid=4698126&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F38 Conclusion: Our data seem to indicate that FISH-determined HER2 status but not p53 is associated with docetaxel sensitivity in metastatic breast cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4698126 Sun, 10 Apr 2011 23:00:00 +0100 4698126 http://www.medworm.com/index.php?rid=4698128&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F36 Background, There has been insufficient examination of the factors affecting long-term survival of more than 5 years in patients with leukemia that is not in remission at transplantation.Method, We retrospectively analyzed leukemia not in remission at allogeneic hematopoietic cell transplantation (allo-HCT) performed at our institution between January 1999 and July 2009. Forty-two patients with a median age of 39 years received intensified conditioning (n=9), standard (n=12) or reduced-intensity conditioning (n=21) for allo-HCT. Fourteen patients received individual chemotherapy for cytoreduction during the three weeks prior to reduced-intensity conditioning. Diagnoses comprised acute leukemia (n=29), chronic myeloid leukemia-accelerated phase (n=2), myelodysplastic syndrome/acute myeloid ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4698128 Sat, 09 Apr 2011 23:00:00 +0100 4698128 http://www.medworm.com/index.php?rid=4698127&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F37 Background, Cancer cells are known to secrete the stress molecules MICA and MICB that activate cytotoxicity by lymphocytes and NK cells through their NKG2D receptor as a mechanism of immunological defense. This work was undertaken to evaluate if cancer cells can also express this receptor as a possible mechanisms of depletion of MIC molecules and thus interfere with their immune recognition. Methods, Myelomonocytic leukemic (TPH-1 and U-937) and cervical cancer (CALO and INBL) cell lines were evaluated by Western Blot, ELISA, flow cytometry and immunocytochemistry to evaluate their capacity to express and secrete MICA and MICB and to be induced to proliferate by these molecules as well as to express their receptor NKG2D. Statistical analysis was performed by two-way ANOVA for time course a... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4698127 Sat, 09 Apr 2011 23:00:00 +0100 4698127 http://www.medworm.com/index.php?rid=4671403&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F35 Conclusion: 125I seed implantation kills pancreatic cancer cells, especially at 4 Gy. 125I-induced apoptosis and changes in DNMT1 and DNMT3b expression suggest potential mechanisms underlying effective brachytherapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4671403 Fri, 01 Apr 2011 23:00:00 +0100 4671403 http://www.medworm.com/index.php?rid=4659106&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F34 Conclusions: We have developed a high through-put screening technique and identified small molecules that stimulate iron uptake both from ferriTf and non-Tf bound iron. These iron facilitator compounds displayed properties suggesting that they may serve as anti-cancer agents. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4659106 Wed, 30 Mar 2011 23:00:00 +0100 4659106 http://www.medworm.com/index.php?rid=4643978&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F32 In the article [Feng Li, Yongmei Liu, Huijiao Chen, Dianying Liao, Yali Shen, Feng Xu and Jin Wang. EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features. Journal of Experimental & Clinical Cancer Research 2011, 30:27] there were errors in Tables 3, 4, 5 6 and 7. The incorrect values were produced due to typographical errors during translation stage. These errors affect neither the published discussion nor the conclusions of the article. However, a few changes to the results section are detailed here. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4643978 Sun, 27 Mar 2011 23:00:00 +0100 4643978 http://www.medworm.com/index.php?rid=4606676&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F30 Conclusion: Our results suggest that a considerable proportion of NSCLC in Chinese population showed discrepancy in KRAS and EGFR mutation status between primary tumors and corresponding metastases. This observation may have important implication for the use of targeted TKI therapy in the treatment of NSCLC patients. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4606676 Thu, 17 Mar 2011 00:00:00 +0100 4606676 http://www.medworm.com/index.php?rid=4581700&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F29 Conclusions: For the first time both crude plant extract from Calomeria amaranthoides and EPD have shown to have potent anti-cancer affects against ovarian cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4581700 Mon, 14 Mar 2011 00:00:00 +0100 4581700 http://www.medworm.com/index.php?rid=4566911&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F28 Conclusion: The combined application of mTOR inhibitor and docetaxel led to a greater degree of cancer cell killing than that by either compound used alone. Therefore, this combination warrants further investigation in its suitability of serving as a novel therapeutic scheme for treating advanced and recurrent lung cancer patients. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4566911 Thu, 10 Mar 2011 00:00:00 +0100 4566911 http://www.medworm.com/index.php?rid=4555056&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F27 Conclusions: COX-2 and EGFR are over-expressed in NSCLC. EGFR is an independent prognostic factor and a predictive factor for radiotherapy response in NSCLC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4555056 Mon, 07 Mar 2011 00:00:00 +0100 4555056 http://www.medworm.com/index.php?rid=4550205&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F26 Conclusions: The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosarcoma. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4550205 Sat, 05 Mar 2011 00:00:00 +0100 4550205 http://www.medworm.com/index.php?rid=4541316&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F25 Conclusions: Anti-Sp17 antibody targeted and accumulated in Sp17 positive tumors in vivo, which demonstrated its capability of serving as a diagnostic reagent. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4541316 Thu, 03 Mar 2011 00:00:00 +0100 4541316 http://www.medworm.com/index.php?rid=4522772&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F24 Conclusions In this mice tumor model, vaccination with mHSP/Ps combined with low-dose CY plus IL-12 induced an immunologic response and a marked antitumor response to autologous tumors. The regimen may be a promising therapeutic agent against tumors. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4522772 Sat, 26 Feb 2011 00:00:00 +0100 4522772 http://www.medworm.com/index.php?rid=4508138&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F22 Conclusion: Both UTI and TAX inhibited the growth of MDA-MB-231 breast carcinoma cells. UTI enhanced the inhibitory effect of TAX by a mechanism consistent with the down-regulated expression of IL-6, IL-8, and TNF-alpha. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4508138 Wed, 23 Feb 2011 00:00:00 +0100 4508138 http://www.medworm.com/index.php?rid=4502599&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F21 Background: Epidemiological studies propose that obesity increases the risk of several cancers, including melanoma. Obesity increases the expression of leptin, a multifunctional peptide produced predominantly by adipocytes which may promote tumor growth. Several recently experiments have suggested that the tumors growth is in need of endothelial progenitor cell (EPC) dependent generation of new blood vessels.Our objectives in the present study were to examine the effects of leptin on melanoma growth, circulating EPCs number and plasma levels of nitric oxide metabolites (NOx). Methods: 2 x 106 B16F10 melanoma cells were injected to thirty two C57BL6 mice subcutaneously. The mice were randomly divided into 4 groups (n=8) in 8th day. Two groups were received twice daily intraperitoneal(i.p) i... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4502599 Mon, 21 Feb 2011 00:00:00 +0100 4502599 http://www.medworm.com/index.php?rid=4484073&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F20 Conclusions: This study demonstrated for the first time that combination of DDP application with miR-451 upregulation might be a potential strategy for the treatment of human NSCLC. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4484073 Thu, 17 Feb 2011 00:00:00 +0100 4484073 http://www.medworm.com/index.php?rid=4484074&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F19 The authors have retracted this article, Journal of Experimental & Clinical Cancer Research 2010, 29:133, as there was a large overlap with a previously published article in International Journal of Cancer 125:1505-1513. Dr Lu ShihHsin, although listed as an author, was not aware of the publication in Journal of Experimental & Clinical Cancer Research and the grant reference number stated in the acknowledgements was incorrectly applied to this article. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4484074 Wed, 16 Feb 2011 00:00:00 +0100 4484074 http://www.medworm.com/index.php?rid=4451219&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F18 Conclusion: Our data indicated that LCMR1, strongly overexpressed in NSCLC, might have applications in the clinical diagnosis and treatment of lung cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4451219 Wed, 09 Feb 2011 00:00:00 +0100 4451219 http://www.medworm.com/index.php?rid=4451220&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F17 Conclusions: Our study indicated that bostrycin had a significant inhibitory effect on proliferation of A549 cells. It is possible that upregulation of microRNA-638 and microRNA-923 and downregulaton of the PI3K/AKT pathway proteins played a role in induction of cell cycle arrest and apoptosis in bostrycin-treated cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4451220 Tue, 08 Feb 2011 00:00:00 +0100 4451220 http://www.medworm.com/index.php?rid=4446598&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F16 Background: This retrospective analysis is focused on the efficacy and safety of radioimmunotherapy (RIT) with Zevalin(R) in nine patients with recurrent follicular lymphoma (FL) who were treated in a consolidation setting after having achieved complete remission or partial remission with FCR. Methods: The median age was 63 yrs (range 46-77), all patients were relapsed with histologically confirmed CD20-positive (grade 1 or 2) FL, at relapse they received FCR every 28 days: F (25mg/m2x 3 days), C (1gr/m2 day 1) and R (375mg/m2 day 4) for 4 cycles. Who achieved at least a partial remission, with (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4446598 Tue, 08 Feb 2011 00:00:00 +0100 4446598 http://www.medworm.com/index.php?rid=4442687&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F15 This study aimed to investigate the molecular structural relationship between cell adhesive molecule CD44 and Lewis y antigen, and determine the effects of Lewis y antigen on CD44-mediated adhesion and spreading of ovarian cancer cell line RMG-I and the Lewis y antigen-overexpressed cell line RMG-I-H. Methods: The expression of CD44 in RMG-I and RMG-I-H cells before and after treatment of Lewis y monoclonal antibody was detected by immunocytochemistry; the expression of Lewis y antigen and CD44 was detected by Western Blot. The structural relationship between Lewis y antigen and CD44 was determined by immunoprecipitation and confocal laser scanning microscopy. The adhesion and spreading of RMG-I and RMG-I-H cells on hyaluronic acid (HA) were observed. The expression of CD44 mRNA in RMG-I a... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4442687 Mon, 07 Feb 2011 00:00:00 +0100 4442687 http://www.medworm.com/index.php?rid=4406589&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F14 This study aims to test the expression of COX-2 and VEGF-C in human gastric cancer, and to analyze the correlation with lymphatic vessel density (LVD), clinicopathologic features and survival prognosis.Methods: Using immunohistochemistry, COX-2, VEGF-C and level of LVD were analyzed in 56 R0-resected primary gastric adenocarcinomas, while paracancerous normal mucosal tissues were also collected as control from 25 concurrent patients. The relationships among COX-2 and VEGF-C expression, LVD, and clinicopathologic parameters were analyzed. The correlations of COX-2, VEGF-C and level of LVD with patient prognosis were also evaluated by univariate tests and multivariate Cox regression.Results: The expression rates of COX-2 and VEGF-C were 69.64% and 55.36%, respectively, in gastric carcinoma. ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4406589 Fri, 28 Jan 2011 00:00:00 +0100 4406589 http://www.medworm.com/index.php?rid=4394886&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F13 Conclusions: COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4394886 Tue, 25 Jan 2011 00:00:00 +0100 4394886 http://www.medworm.com/index.php?rid=4383355&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F12 The immune system in patients detects and eliminates tumor cells, but tumors still progress persistently. The mechanisms by which tumor cells survive under the pressure of immune surveillance are not fully understood. This review is to present the evidence from clinical studies, showing a significant correlation of clinicopathological features of carcinoma with: (1) the loss of classical human leukocyte antigen class I, (2) the up-regulation of non-classical human leukocyte antigen class I, pro-apoptotic Fas ligand and receptor-binding cancer antigen expressed on SiSo cells I, and (3) the formation of immunosuppressive microenvironment by up-regulation of transforming growth factor-beta, Galectin-1, inhibitory ligand B7s, indoleamine 2,3-dioxygenase and arginase, as well as by recruitment ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4383355 Fri, 21 Jan 2011 00:00:00 +0100 4383355 http://www.medworm.com/index.php?rid=4373189&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F11 Streptococcus bovis (S. bovis) bacteria are associated with colorectal cancer and adenoma. S. bovis is currently named S. gallolyticus. Twenty five to 80% of patients with S. bovis/gallolyticus bacteremia have concomitant colorectal tumors. Colonic neoplasia may arise years after the detection of bacteremia or infectious endocarditis of S. bovis/gallolyticus. The presence of S. bovis/gallolyticus bacteremia and/or endocarditis is also related to the presence of villous or tubular-villous adenomas in the large intestine. In addition, serological relationship of S. gallolyticus with colorectal tumors and direct colonization of S. gallolyticus in tissues of colorectal tumors were found. However, this association is still under controversy and has long been underestimated. Moreover, the etiolo... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4373189 Thu, 20 Jan 2011 00:00:00 +0100 4373189 http://www.medworm.com/index.php?rid=4362334&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F10 Backgroun:To evaluate the therapeutic strategies commonly employed in the clinic for the management of brain metastases (BMs) and to correlate disease outcome with type of treatment and therapeutic resources available at the treating center. Methods: Four Cancer centers participated to the survey. Data were collected through a questionnaire filled in by one physician for each centre. Results: Clinical data regarding 290 cancer patients with BMs from solid tumors were collected. Median age was 59 and 59% of patients had [less than or equal to] 3 brain metastases. A local approach (surgery and stereotactic radiosurgery) was adopted in 31% of patients. The local approach demonstrated to be superior in terms of survival compared to the regional/systemic approach (whole brain radiotherapy and c... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4362334 Tue, 18 Jan 2011 00:00:00 +0100 4362334 http://www.medworm.com/index.php?rid=4356280&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F9 Stem cells are a relevant source of information about cellular differentiation, molecular processes and tissue homeostasis, but also one of the most putative biological tools to treat degenerative diseases. This review focuses on human stem cells clinical and experimental application. Our aim is to take a correct view of the available stem cell subtypes and their rational use in the medical area, with a specific focus on their therapeutic benefits and side effects. We have reviewed the main clinical trials dividing them basing on their clinical applications, and taking into account the ethical issue associated with the stem cell therapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4356280 Mon, 17 Jan 2011 00:00:00 +0100 4356280 http://www.medworm.com/index.php?rid=4353287&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F8 Background: Hedgehog (Hh) signaling pathway is involved in regulation of many tissues development and oncogenesis. Recently, Hh signaling has been identified as a required functional pathway for leukemia stem cells(LSCs), and loss of this pathway impairs leukemia progression. Objectives:The aim of this study was to determine the expression of Hedgehog signaling molecules in Chronic Myelogeneous Leukemia (CML) patients and normal people by semiquantitative polymerase chain reaction (PCR) and to correlate mRNA expression to patients' clinical data. Results:Here, we showed that Sonic hedgehog (Shh), Smoothened (Smo), and Gli1 genes of Hh signaling were significantly upregulated in CML patients when compared with normal people (P (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4353287 Sun, 16 Jan 2011 00:00:00 +0100 4353287 http://www.medworm.com/index.php?rid=4338943&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F7 Conclusions: By utilizing LINACs installed in the Radiotherapy Department it is possible to provide an internal blood component irradiation service, capitalizing on internal resources without any inconvenience/discomfort to patients undergoing radiotherapy and satisfying governmental regulatory requirements. The internal irradiation procedures has proven to be safe and feasible, and along with the significant cost/time reduction suggests that it is more advantageous than external procedures. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4338943 Wed, 12 Jan 2011 00:00:00 +0100 4338943 http://www.medworm.com/index.php?rid=4322915&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F5 Conclusions: These findings suggest that genetic variations in SULF1 may play a role in ovarian cancer onset and prognosis. Further studies with large sample sizes and of the mechanistic relevance of SULF1 SNPs are warranted. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4322915 Fri, 07 Jan 2011 00:00:00 +0100 4322915 http://www.medworm.com/index.php?rid=4319207&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F4 Conclusion: Adrenaline is more effective than hyperthermia in order to enhance the intratumoral concentration of cisplatin in rats with peritoneal carcinomatosis from ovarian origin. It may also decrease the systemic absorption of the drug. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4319207 Fri, 07 Jan 2011 00:00:00 +0100 4319207 http://www.medworm.com/index.php?rid=4319208&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F3 Conclusions: This study offers a plausible mechanism to explain the increased risk for oral cancer associated with high acetaldehyde concentrations in certain beverages. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4319208 Thu, 06 Jan 2011 00:00:00 +0100 4319208 http://www.medworm.com/index.php?rid=4314727&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F1 Background Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti-cancer drugs. Elimination of template function of telomerase RNA may repress the telomerase activity. Methods A pseudo- knotted HDV ribozyme (g.RZ57) directed against the RNA component of human telomerase (hTR) was designed and synthesized. An in vitro transcription plasmid and a eukaryotic expression plasmid of ribozyme were constructed. The eukaryotic expression plasmid was induced into heptocellular carcinoma 7402 cells, colon cancer HCT116 cells and L02 hepatocytes respectively. Then we determine the cleavage activity of ribozyme against human telomerase RNA component (hTR) both in vitro and in vivo, and detect telomerase activity continuously. Results HDV... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4314727 Thu, 06 Jan 2011 00:00:00 +0100 4314727 http://www.medworm.com/index.php?rid=4314726&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F30%2F1%2F2 Conclusion: Our data suggest that specific immunotherapy targeted CTAs might be a novel treatment option for IHCC patients. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4314726 Thu, 06 Jan 2011 00:00:00 +0100 4314726 http://www.medworm.com/index.php?rid=4300925&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F174 Conclusions We identified a subset of miRNAs that were differentially expressed in BCSCs, providing a starting point to explore the functions of these miRNAs. Evaluating characteristic BCSC miRNAs represents a new method for studying breast cancer-initiating cells and developing therapeutic strategies aimed at eradicating the tumourigenic subpopulation of cells in breast cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4300925 Fri, 31 Dec 2010 00:00:00 +0100 4300925 http://www.medworm.com/index.php?rid=4300924&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F175 Conclusions: In essence, compound 1i showed potential as an antitumor agent. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4300924 Fri, 31 Dec 2010 00:00:00 +0100 4300924 http://www.medworm.com/index.php?rid=4298981&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F172 Conclusion: Our data first demonstrate that c-Myb has a functionally important role in the regulation of OPN expression in HCC cells, suggesting that c-Myb might be a new target to control HCC metastasis. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4298981 Thu, 30 Dec 2010 00:00:00 +0100 4298981 http://www.medworm.com/index.php?rid=4298980&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F173 Conclusions: As single agents, all drugs showed an anti-tumor effect in GIST xenografts but everolimus was superior. The everolimus plus imatinib combination appeared to be the most active regimen both in terms of inhibiting tumor growth and tumor metabolism. The integration of everolimus in GIST treatment merits further investigation. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4298980 Thu, 30 Dec 2010 00:00:00 +0100 4298980 http://www.medworm.com/index.php?rid=4295385&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F171 Conclusion: These data suggest that these markers are regulated differentially and the difference in their expression showed a correlation with malignant transformation. With regard to the duality PSMA-PSA, this implies the significance of their investigation together in normal and pathologic prostate tissues. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4295385 Tue, 28 Dec 2010 00:00:00 +0100 4295385 http://www.medworm.com/index.php?rid=4282081&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F169 Conclusions: Our results suggest that LCSCs may have a distinct miRNA expression fingerprint during hepatocarcinogenesis. Dissecting these relationships will provide a new understanding of the function of miRNA in the process of neoplastic transformation of LCSCs. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4282081 Thu, 23 Dec 2010 00:00:00 +0100 4282081 http://www.medworm.com/index.php?rid=4282080&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F170 ObjectiveThe purpose of the study was to explore the anti-tumor effect of ultrasound-targeted microbubble destruction mediated herpes simplex virus--thymidine kinase (HSV-TK) suicide gene system on mice hepatoma. Methods: Forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished:(1)PBS;(2) HSV-TK (3) HSV-TK+ultrasound(HSV-TK + US);(4)HSV-TK+ultrasound+microbubbles(HSV-TK+US+MB).The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis.At last, the inhibition rates and survival time of the animals were compared among all groups. Results: The TK protein expression of HSV-TK+MB+US group in tumor-bearing mice tissues were significantly higher than those in other... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4282080 Thu, 23 Dec 2010 00:00:00 +0100 4282080 http://www.medworm.com/index.php?rid=4272795&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F168 Conclusions: The Italian research institutions in the field of oncology are moving the first steps towards the philosophy of OA. The main effort should be the implementation of common procedures also in order to connect scientific publications to researchers curricula. In this framework, an important effort is represented by the project of ISS aimed to set a common interface able to allow migration of data from partner institutions to the OA compliant repository DSpace ISS. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4272795 Mon, 20 Dec 2010 00:00:00 +0100 4272795 http://www.medworm.com/index.php?rid=4267171&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F166 Background: ChromograninA in prostate carcinoma (PC) indicate NE differentiation. This tumour is more aggressive and resistant to hormone therapy. Patients and methods: We analyzed the incidence of pre-operative ChromograninA serum levels in non metastatic PC patients. Serum PSA and ChromograninA were analyzed before treatment. Clinicopathological parameters were evaluated in relation to serum ChromograninA. 486 patients were enrolled. Results: We found 352 pT2 and 134 pT3. 21 patients were N+. 278 patients had Gleason score levels 7. Median PSA pre-operative level was 7.61 ng/ml. PSA was significantly associated with pT stage (pT2 with PSA abnormal 23.6% vs pT3 48.5%, p7 vs 29.5 % in the Gleason score =7 vs 27.3 % in the Gleason score (Source: Journal of Experimental and Clinical Cancer R... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4267171 Fri, 17 Dec 2010 00:00:00 +0100 4267171 http://www.medworm.com/index.php?rid=4267170&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F167 In this study, I sought to characterize the downstream apoptotic genes targeted by CH in MCF-7 human breast cancer cells. CH effectively kills MCF-7 cells through induction of apoptosis. Apoptosis was confirmed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and real-time PCR assays. Cells were exposed to 150 ug/ml CH and 300 ug/mL CH for 24 hours, which resulted in 40.7% and 41.16% apoptotic cells, respectively. Moreover, a 48-hour exposure to 150 ug/ml CH and 300 ug/ml CH resulted in 43.73% and 52.95% apoptotic cells, respectively. Interestingly, after 72 hours of exposure to both concentrations of CH, almost 100% of cells lost their integrity. These results were further confirmed by the increased expression of caspase-3,-8, and -9 and TP53 in a time-depe... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4267170 Fri, 17 Dec 2010 00:00:00 +0100 4267170 http://www.medworm.com/index.php?rid=4267172&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F165 This study was conducted to investigate the effect of all-trans retinoic acid (ATRA) on GIST cell lines. Methods: Cell proliferation was determined by trypan blue dye exclusion test. Western blot analysis was performed to test the expression of activated KIT, its downstream proteins, and apoptosis associated proteins. The cytotoxic interactions of imatinib with ATRA were evaluated using the isobologram of Steel and Peckham.Results and conclusionIn this work, for the first time we have demonstrated that ATRA affected on cell proliferation of GIST-T1 and GIST-882 cell line through inhibition of cell growth in a dose dependent manner and induced apoptosis. High dose of ATRA induced morphologic change in GIST-T1 cells, rounded-up cells, and activated the caspase-3 protein. In further examinati... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4267172 Thu, 16 Dec 2010 00:00:00 +0100 4267172 http://www.medworm.com/index.php?rid=4267173&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F164 More data about TACE and pTACE seem necessary to better define the global treatment strategy for HCC. Aim of our analysis was to evaluate the role of TACE, either with lipiodol (traditional) or drug-eluting microspheres in terms of response rate (RR), time to progression (TTP), overall survival (OS) and toxicity in HCC.Patients with HCC undergoing traditional TACE or pTACE (either alone or in combination with other treatment options) were eligibleOne hundred and fifty patients were analyzed. In the global patient population median OS was 46 months for lipiodol TACE and 19 months for pTACE (p (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4267173 Wed, 15 Dec 2010 00:00:00 +0100 4267173 http://www.medworm.com/index.php?rid=4253598&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F163 Conclusions: We conclude that HPB-AML-I cells appear to be unique neoplastic cells, which may be derived from MSCs, but are not hematopoietic progenitor cells. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4253598 Mon, 13 Dec 2010 00:00:00 +0100 4253598 http://www.medworm.com/index.php?rid=4249605&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F161 Conclusions: Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4249605 Sat, 11 Dec 2010 00:00:00 +0100 4249605 http://www.medworm.com/index.php?rid=4249604&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F162 Conclusions: XAF1 down-regulation may contribute to the prostate cancer development. The enhanced XAF1 expression by somatostatin indicates a promising strategy for prostate cancer therapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4249604 Sat, 11 Dec 2010 00:00:00 +0100 4249604 http://www.medworm.com/index.php?rid=4249606&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F160 Conclusions: Synthetic as-APF inhibits cell proliferation in T24 bladder carcinoma cells via the CKAP4 receptor. The mechanism for this inhibition involves regulating phosphorylation of specific cell signaling molecules (Akt, GSK3beta, and beta-catenin) plus mRNA and protein expression of p53 and MMP2. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4249606 Fri, 10 Dec 2010 00:00:00 +0100 4249606 http://www.medworm.com/index.php?rid=4242441&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F159 Conclusions: These results suggest that saikosaponins sensitize cancer cells to cisplatin through ROS-mediated apoptosis, and the combination of saikosaponins with cisplatin could be an effective therapeutic strategy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4242441 Thu, 09 Dec 2010 00:00:00 +0100 4242441 http://www.medworm.com/index.php?rid=4238587&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F158 Conclusion: Expression of HIF-1alpha and MRP1 was observed in most chordoma specimen and CM-319 cell line; expression of HIF-1alpha correlated with MRP1. HIF-1alpha and MRP1 may play a role in the multidrug resistance of chordoma to chemotherapy. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4238587 Wed, 08 Dec 2010 00:00:00 +0100 4238587 http://www.medworm.com/index.php?rid=4230739&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F157 Conclusions: The weakness of our cohort size notwithstanding, vitamins levels in serum and genetic polymorphisms in the hOGG1 or GST genes do not appear to be important modulators of 8-oxodG levels. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4230739 Mon, 06 Dec 2010 00:00:00 +0100 4230739 http://www.medworm.com/index.php?rid=4206366&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F156 This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. Methods: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. Results: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4206366 Sat, 27 Nov 2010 00:00:00 +0100 4206366 http://www.medworm.com/index.php?rid=4203727&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F154 Conclusions: These results indicate that inhibition of GSK-3beta downregulates the NF-kappaB activation pathway, leading to suppression of the expression of an NF-kappaB-regulated gene and promotion of apoptosis in ALL cells in vitro. Furthermore, our findings suggest that GSK-3beta or NF-kappaB is a potential therapeutic target in the treatment of pediatric ALL. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4203727 Fri, 26 Nov 2010 00:00:00 +0100 4203727 http://www.medworm.com/index.php?rid=4203726&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F155 Conclusion: Genetic polymorphisms in the D-loop are independent prognostic markers for patients with ESCC. Accordingly, the analysis of genetic polymorphisms in the mitochondrial D-loop can help identify patient subgroups at high risk of a poor disease outcome. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4203726 Fri, 26 Nov 2010 00:00:00 +0100 4203726 http://www.medworm.com/index.php?rid=4191474&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F153 Conclusion: The FU-MFH-2 cell line will be a particularly useful model for studying molecular pathogenesis of human pleomorphic MFH. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4191474 Wed, 24 Nov 2010 00:00:00 +0100 4191474 http://www.medworm.com/index.php?rid=4191475&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F152 Conclusions: This noninvasive, novel combination of UTMD with PEI could enhance targeted gene delivery and gene expression in tumor xenografts at intravenous administration effectively without causing any apparently adverse effect, and might be a promising candidate for gene therapy. Silencing of survivin gene expression with shRNA could be facilitated by this non-viral technique, and lead to significant cell apoptosis. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4191475 Tue, 23 Nov 2010 00:00:00 +0100 4191475 http://www.medworm.com/index.php?rid=4191476&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F151 MicroRNAs are important gene regulators that potentially play a profound role in tumorigenesis. Increasing evidence indicates that miR-145 is a tumor suppressor capable of inhibiting breast and colon cancer cell growth both in vitro and in vivo. However, the biological function of miR-145 in non-small cell lung cancer (NSCLC) is largely unknown. In colon cancer cells, c-Myc is a confirmed direct target for miR-145. The aim of this work was to investigate the effect of miR-145 and c-Myc on proliferation of NSCLC cells, using the NSCLC cell lines A549 and H23 as models. We determined the expression level of miR-145 in tumor tissues relative to adjacent non-tumor tissues, and in NSCLC cell lines relative to non-malignant lung cells. Downregulation of miR-145 was seen in tumor tissues and the ... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4191476 Mon, 22 Nov 2010 00:00:00 +0100 4191476 http://www.medworm.com/index.php?rid=4176103&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F150 Conclusion: Our data suggests that rapamycin can effectively reverse GC resistance in T-ALL and this effect is achieved by inducing cell cycles arrested at G0/ G1 phase and activating the intrinsic apoptotic program. Therefore, combination of mTOR inhibitor rapamycin with GC containing protocol might be an attracting new therapeutic approach for GC resistant T-ALL patients. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4176103 Thu, 18 Nov 2010 00:00:00 +0100 4176103 http://www.medworm.com/index.php?rid=4172584&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F147 Conclusions: This study shows that genetic aberrations near SOSTDC1 are not uncommon in renal cancer, and occur in adult as well as pediatric renal tumors. These observations of SOSTDC1 LOH, however, did not correspond with changes in SOSTDC1 protein levels or signaling regulation. Although our conclusions are limited by sample size, we suggest that an alternative mechanism such as epigenetic silencing of SOSTDC1 may be a key contributor to the reduced SOSTDC1 mRNA and protein levels observed in renal cancer. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4172584 Tue, 16 Nov 2010 00:00:00 +0100 4172584 http://www.medworm.com/index.php?rid=4172583&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F148 Background: Colloidal silver has been used as an antimicrobial and disinfectant agent. However, there is scarce information on its antitumor potential. The aim of this study was to determine if colloidal silver had cytotoxic effects on MCF-7 breast cancer cells and its mechanism of cell death. Methods: MCF-7 breast cancer cells were treated with colloidal silver (ranged from 1.75 to 17.5 ng/mL) for 5h at 37oC and 5% CO2 atmosphere. Cell Viability was evaluated by trypan blue exclusion method and the mechanism of cell death through detection of mono-oligonucleosomes using an ELISA kit and TUNEL assay. The production of NO, LDH, and Gpx, SOD, CAT, and Total antioxidant activities were evaluated by colorimetric assays. Results: Colloidal silver had dose-dependent cytotoxic effect in MCF-7 bre... Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4172583 Tue, 16 Nov 2010 00:00:00 +0100 4172583 http://www.medworm.com/index.php?rid=4168458&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F146 Conclusions By connecting the cellular proteins targeted by HBV, we have constructed a central network of proteins associated with hepatocellular carcinoma which might be to regard as the basis of a detailed map for tracking new cellular interactions, and guiding future investigations. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4168458 Tue, 16 Nov 2010 00:00:00 +0100 4168458 http://www.medworm.com/index.php?rid=4144679&cid=s_37196_6_f&fid=37196&url=http%3A%2F%2Fwww.jeccr.com%2Fcontent%2F29%2F1%2F145 Conclusion: These results indicate that NCTD induced cytotoxicity in HepG2 cells by apoptosis,which is mediated through ROS generation and mitochondrial pathway. (Source: Journal of Experimental and Clinical Cancer Research) Journal of Experimental and Clinical Cancer Research journals http://www.medworm.com/rss/comments.php?id=4144679 Tue, 09 Nov 2010 00:00:00 +0100 4144679