MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Molecular Signaling' source. Thu, 09 Feb 2012 16:56:47 +0100 http://www.medworm.com/index.php?rid=5635464&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F7%2F1%2F3 Dopaminergic inputs are sensed on the cell surface by the seven-transmembrane dopamine receptors that belong to a superfamily of G-protein-coupled receptors (GPCRs). Dopamine receptors are classified as D1-like or D2-like receptors based on their homology and pharmacological profiles. In addition to well established G-protein coupled mechanism of dopamine receptors in mammalian system they can also interact with other signaling pathways. In C. elegans four dopamine receptors (dop-1, dop-2, dop-3 and dop-4) have been reported and they have been implicated in a wide array of behavioral and physiological processes. We performed this study to assign the signaling pathway for DOP-2, a D2-like dopamine receptor using a split-ubiquitin based yeast two-hybrid screening of a C. elegans cDNA library... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5635464 Thu, 26 Jan 2012 05:00:00 +0100 5635464 http://www.medworm.com/index.php?rid=5624812&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F7%2F1%2F2 Conclusions: Most, if not all, of the TrkB receptor has a preformed, yet inactive, homodimeric structure before BDNF binding. The intracellular domain of TrkB plays a crucial role in the spontaneous dimerization of the newly synthesized receptors, which occurs in ER. These findings provide new insight into an understanding of a molecular mechanism underlying transmembrane signaling mediated by NT receptors. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5624812 Tue, 24 Jan 2012 05:00:00 +0100 5624812 http://www.medworm.com/index.php?rid=5568908&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F7%2F1%2F1 Paget's disease of bone (PDB) is a skeletal disorder characterized by focal and disorganized increases in bone turnover and overactive osteoclasts. The discovery of mutations in the SQSTM1/p62 gene in numerous patients has identified protein p62 as an important modulator of bone turnover. In both precursors and mature osteoclasts, the interaction between receptor activator of NF-kappaB ligand (RANKL) and its receptor RANK results in signaling cascades that ultimately activate transcription factors, particularly NF-kappaB and NFATc1, promoting and regulating the osteoclast differentiation, activity, and survival. As a scaffold with multiple protein-protein interaction motifs, p62 is involved in virtually all the RANKL-activated osteoclast signaling pathways, along with being implicated in n... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5568908 Wed, 04 Jan 2012 05:00:00 +0100 5568908 http://www.medworm.com/index.php?rid=5292957&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F12 Conclusions: These results suggest that various Wnt ligands control subcellular beta-catenin localization, which regulate myoblast proliferation and myotube formation. Wnt signaling via beta-catenin likely acts as a molecular switch that regulates the transition from cell proliferation to myogenic differentiation. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5292957 Wed, 05 Oct 2011 04:00:00 +0100 5292957 http://www.medworm.com/index.php?rid=5292956&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F13 Conclusion: Data presented here suggest that GC-mediated upregulation of E4BP4 facilitates Bim upregulation and apoptosis of CEM cells. Since the Bim promoter does not contain any consensus GRE or EBPRE sequences, induction of Bim may be a secondary response. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5292956 Wed, 05 Oct 2011 04:00:00 +0100 5292956 http://www.medworm.com/index.php?rid=5191223&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F11 Conclusions: These findings suggest that P-Rex1 is not required for Rac1-mediated platelet activation and that the GEF activities of P-Rex1 may be more specific to GPCR chemokine receptor mediated processes in immune cells and tumor cells. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5191223 Wed, 31 Aug 2011 23:00:00 +0100 5191223 http://www.medworm.com/index.php?rid=5191224&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F10 ConclusionGPKOW is a RNA-binding protein that binds RNA in a PKA regulated fashion. Together with our previous results demonstrating that PKA regulates pre-mRNA splicing, our results suggest that PKA-phosphorylation is involved in regulating RNA processing at several steps. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5191224 Tue, 30 Aug 2011 23:00:00 +0100 5191224 http://www.medworm.com/index.php?rid=5124421&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F9 ${item.shortDescription} (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5124421 Thu, 11 Aug 2011 23:00:00 +0100 5124421 http://www.medworm.com/index.php?rid=5117132&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F8 ${item.shortDescription} (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5117132 Tue, 09 Aug 2011 23:00:00 +0100 5117132 http://www.medworm.com/index.php?rid=5048330&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F7 ${item.shortDescription} (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5048330 Tue, 19 Jul 2011 23:00:00 +0100 5048330 http://www.medworm.com/index.php?rid=5048331&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F6 ${item.shortDescription} (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=5048331 Sun, 17 Jul 2011 23:00:00 +0100 5048331 http://www.medworm.com/index.php?rid=4827629&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F4 Conclusion: Our results suggest that in HEK293 cells MK2 is the HSP27 kinase engaged in stress-induced, but not cAMP-induced phosphorylation of HSP27, while MK5 seems to be the sole MK to mediate HSP27 phosphorylation in response to stimulation of the PKA pathway. Thus, despite the same substrate specificity towards HSP27, MK2 and MK5 are implicated in different signaling pathways causing actin reorganization. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4827629 Sun, 15 May 2011 23:00:00 +0100 4827629 http://www.medworm.com/index.php?rid=4801622&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F3 Conclusion: Both AKAP5 and AKAP12 display the capacity to form supermolecular homo-oligomeric structures that likely influence the localization and function of these molecular scaffolds. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4801622 Sun, 08 May 2011 23:00:00 +0100 4801622 http://www.medworm.com/index.php?rid=4544424&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F2 The K-RAS oncogene is widely mutated in human cancers. Activating mutations in K-RAS give rise to constitutive signalling through the MAPK/ERK and PI3K/AKT pathways promoting increased cell division, altered apoptosis and transformation. The majority of activating mutations in K-RAS are located in codons 12 and 13. In a colorectal cancer we identified a novel K-RAS co-mutation that altered codons 19 and 20 resulting in transitions at both codons (L19F/T20A) in the same allele. Using focus forming transformation assays in vitro , we showed that co-mutation of L19F/T20A in K-RAS demonstrated intermediate transforming ability that was greater than that of individual L19F and T20A mutants, but less than that of G12D and G12V K-RAS mutants. This demonstrated the synergistic effects of co-mutati... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4544424 Thu, 03 Mar 2011 00:00:00 +0100 4544424 http://www.medworm.com/index.php?rid=4337085&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F6%2F1%2F1 Conclusion: Dvl3-based punctae/signalsomes made visible by fluorescent microscopy now can be interrogated by advanced physical means, defining such properties as signalsome Mr/MW, molecular composition, and intracellular locale. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4337085 Tue, 11 Jan 2011 00:00:00 +0100 4337085 http://www.medworm.com/index.php?rid=4257995&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F20 Conclusion: These studies indicate that TRG-induced apoptosis is modulated by PI3K pathway in a novel Akt-independent manner, which might contribute to its tumor promoting effects. Since PI3K activation is linked with various cancers, combination therapy utilizing TRG and PI3K inhibitors has the potential to not only increase the efficacy of TRG as a chemotherapeutic agent but also reduce its off target effects. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4257995 Mon, 13 Dec 2010 00:00:00 +0100 4257995 http://www.medworm.com/index.php?rid=4196281&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F19 Conclusions: The C-terminal third of Dvl3 and His single amino acid repeats 637,638 and 647,648 (which are unique to and conserved in Dvl3) are essential for Wnt5a activation of the non-canonical pathway, but not for the Wnt3a activation of the canonical pathway. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4196281 Tue, 23 Nov 2010 00:00:00 +0100 4196281 http://www.medworm.com/index.php?rid=4132531&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F18 Conclusion: These data, together with phase I/II clinical data showing tolerability of TLN-4601, support conducting a clinical trial in advanced pancreatic cancer patients. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4132531 Tue, 02 Nov 2010 00:00:00 +0100 4132531 http://www.medworm.com/index.php?rid=4011563&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F17 Conclusions: Overexpressing the adaptor protein SH2B1beta enhanced H2O2-induced PI3K-AKT and MEK-ERK1/2 signaling, reduced nucleus-localized FoxOs and the expression of a pro-apoptotic gene, FasL. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=4011563 Sun, 26 Sep 2010 23:00:00 +0100 4011563 http://www.medworm.com/index.php?rid=3998005&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F16 Conclusions: Overall, our results suggest that variations among receptor oligomers occur early in the synthesis/maturation processes, and that chaperones will interact more specifically with some receptor pairs than others to allow the formation of certain receptor pairs, while others will contribute to the folding and maturation of receptors without any effect on receptor assembly within a signaling complex. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3998005 Thu, 23 Sep 2010 23:00:00 +0100 3998005 http://www.medworm.com/index.php?rid=3998006&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F15 Conclusions: Our study demonstrates that AMPK activates Akt through IGF-1R dependent and independent mechanisms. Co-targeting IGF-1R and related downstream metabolic and oncogenic signaling pathways represent a potential strategy for future translation into novel ALL therapies. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3998006 Wed, 22 Sep 2010 23:00:00 +0100 3998006 http://www.medworm.com/index.php?rid=3880284&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F14 Conclusion: Since carcinogenesis is a complex process, combination of bioactive dietary agents with complementary activities will be beneficial for prostate cancer prevention and/or treatment. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3880284 Tue, 17 Aug 2010 23:00:00 +0100 3880284 http://www.medworm.com/index.php?rid=3861425&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F12 Conclusion: CD437 regulates cell growth in part by regulating stability of p21WAF1/CIP1 mRNA that involves specific RNA-protein interactions that are phosphorylation-dependent, while not requiring nascent transcription or protein synthesis. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3861425 Wed, 11 Aug 2010 23:00:00 +0100 3861425 http://www.medworm.com/index.php?rid=3783763&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F11 Conclusions: Our data suggest that coordinated activation of Galphaq and Galphai may link the tP2YR and possibility the Mas oncogene with signaling pathways resulting in activation of Rho family proteins to promote cellular transformation. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3783763 Thu, 22 Jul 2010 23:00:00 +0100 3783763 http://www.medworm.com/index.php?rid=3766754&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F10 Conclusion: These data suggest that inhibition of PI3K/AKT and ERK pathways activated FOXO transcription factors and enhanced SFN-induced FOXO transcriptional activity, leading to cell cycle arrest and apoptosis in pancreatic cancer cells. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3766754 Sun, 18 Jul 2010 23:00:00 +0100 3766754 http://www.medworm.com/index.php?rid=3746353&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F8 Breast cancers show a lack of response to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), despite 30% of tumors expressing EGFR. The mechanism of this resistance is unknown; however, we have recently shown that Met kinase activity compensates for loss of EGFR kinase activity in cell culture models. Met has been implicated in the pathogenesis of breast tumors and therefore may cooperate with EGFR for tumor growth. Here we have found that EGFR phosphorylation and cell proliferation is in part regulated by Met expression. In addition, we found that Met constitutive phosphorylation occurred independent of the Met ligand hepatocyte growth factor (HGF). Ligand-independent Met phosphorylation is mediated by Met amplification, mutation, or overexpression and by Met inte... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3746353 Sun, 11 Jul 2010 23:00:00 +0100 3746353 http://www.medworm.com/index.php?rid=3717954&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F7 Conclusion: CARP-1 threonine667 regulates H89-dependent signaling by a novel pathway that involves modulation of CARP-1 interaction with TAZ and transcriptional down-regulation of c-myc. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3717954 Wed, 30 Jun 2010 23:00:00 +0100 3717954 http://www.medworm.com/index.php?rid=3655285&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F6 Transient expression of adenoviral oncoprotein E1B55K in normal cells induces aggresome formation and sequestration of critical host proteins in aggresomes. Our previous studies reported that Sequence Specific Binding Protein 2 (SSBP2), a candidate tumor suppressor is recruited to aggresomes in adenovirally transformed human embryonal kidney 293 (HEK293) cells[1]. To understand the extent and significance of the E1B55K-SSBP2 interactions in these cells, we have examined SSBP2 localization under conditions of stress in HEK293 cells. SSBP2 localizes to PML- Nuclear Bodies (PML-NBs) in response to inhibition of nuclear export, treatment with etoposide, hydroxyurea or gamma irradiation only in HEK293 cells. Furthermore, the PML-NBs grow in size and number in response to radiation over a 24 h... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3655285 Thu, 10 Jun 2010 23:00:00 +0100 3655285 http://www.medworm.com/index.php?rid=3602982&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F5 Conclusions: Taken together, these results suggest that Zn2+ is sequestered into thapsigargin/IP3-sensitive stores and is released upon agonist stimulation. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3602982 Tue, 25 May 2010 23:00:00 +0100 3602982 http://www.medworm.com/index.php?rid=3548818&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F4 Conclusions: These results demonstrate that NSC109268 enhances sensitivity of ovarian cancer 2008 cells to cisplatin independent of p53. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3548818 Sun, 09 May 2010 23:00:00 +0100 3548818 http://www.medworm.com/index.php?rid=3493765&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F3 Conclusion: The dynamic phosphorylation of AKAP12 "biosensed" by AKAR2-AKAP12 reveals the scaffold in association with the cell membrane, undergoing rapid phosphorylation by PKA. The perinuclear, cytoplasmic accumulation of phosphorylated scaffold reflects the phosphorylated, PKA-activated form of AKAP12, which catalyzes the resensitization and recycling of desensitized, internalized G-protein-coupled receptors. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3493765 Wed, 21 Apr 2010 23:00:00 +0100 3493765 http://www.medworm.com/index.php?rid=3297846&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F2 Conclusions: We have effectively utilized C. elegans as an in vivo genetic system to evaluate the activity and selectivity of inhibitors intended to target the Ras-Raf-MAPK pathway. We demonstrated the ability of MCP110 to disrupt, at the level of Ras/Raf, the Muv phenotype induced by chronic activation of this pathway in C. elegans. In mammalian cells, we not only demonstrated MCP-mediated blockade of the physical interaction between Ras and Raf, but also narrowed the site of interaction on Raf to the RBD, and showed consequent functional impairment of the Ras-Raf-MEK-ERK pathway in both in vivo and cell-based systems. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3297846 Tue, 23 Feb 2010 00:00:00 +0100 3297846 http://www.medworm.com/index.php?rid=3287230&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F5%2F1%2F1 Conclusion Our present study demonstrates that AMPK exerts dual effects on the PI3K pathway, stimulating PI3K/Akt and inhibiting mTOR/S6K. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3287230 Thu, 18 Feb 2010 00:00:00 +0100 3287230 http://www.medworm.com/index.php?rid=3128757&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F8 The tuberous sclerosis complex (TSC) is caused by defects in one of two tumor suppressor genes, TSC-1 or TSC-2. TSC-2 gene encodes tuberin, a protein involved in the pathogenesis of kidney tumors, both angiomyolipomas and renal cell carcinomas. On the other hand, mice-deficient in the DNA repair enzyme OGG1 spontaneously develop adenoma and carcinoma. Downregulation of tuberin results in a marked decrease of OGG1 and accumulation of oxidative DNA damage, (8-oxodG) in cultured cells. In addition, tuberin haploinsufficiency is associated with the loss of OGG1 and accumulation of 8-oxodG in rat kidney tumor. Deficiency in tuberin results in decreased OGG1 and NF-YA protein expression and increased 8-oxodG in kidney tumor from TSC patients. In the current study, molecular mechanisms by which t... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=3128757 Tue, 29 Dec 2009 00:00:00 +0100 3128757 http://www.medworm.com/index.php?rid=2831565&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F7 Conclusion: These findings indicate that the hIP is post-translationally modified by ubiquitination, which targets the immature species to the 26S proteasomal degradation pathway and the mature species to the lysosomal degradation pathway. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2831565 Thu, 24 Sep 2009 23:00:00 +0100 2831565 http://www.medworm.com/index.php?rid=2633793&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F4 Conclusion: Our data demonstrate that the expression level of TIMP-2 protein can directly modulate the NF-kappaB pathway in human melanoma cells. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2633793 Wed, 22 Jul 2009 23:00:00 +0100 2633793 http://www.medworm.com/index.php?rid=2413376&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F3 Conclusions: Our results seemed to implicate the oncosuppressor BRCA2 and the phytoestrogen pathways in different nuclear gene expressions via an ER-independent manner. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2413376 Thu, 14 May 2009 04:00:00 +0100 2413376 http://www.medworm.com/index.php?rid=2402584&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F2 Conclusion: Phytoestrogens were much more potent in mediating these nongenomic responses (activation of MAPKs, PRL release, and increased intracellular [Ca2+]) via mERα than was previously reported for genomic responses. The unique non-monotonic dose responses and variant signaling patterns caused by E2 and all tested phytoestrogens suggest that complex and multiple signaling pathways or binding partners could be involved. By activating these different nongenomic signaling pathways, phytoestrogens could have significant physiological consequences for pituitary cell functions. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2402584 Tue, 28 Apr 2009 04:00:00 +0100 2402584 http://www.medworm.com/index.php?rid=2295773&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F4%2F1%2F1 Conclusions: The MAP kinase and CDC2 kinase-dependent intracellular mechanisms are involved in or are the targets of the GH's action processes, and these activities are probably directly or indirectly modulated by AKT/PKB pathways. We propose that the AKT/PKB-eNOS module likely functions as a negative feedback mediator of GH actions. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2295773 Wed, 25 Mar 2009 04:00:00 +0100 2295773 http://www.medworm.com/index.php?rid=2222043&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F20 Conclusion: These findings indicate that ubiquitination of β-arrestin has a distinct role in the differential trafficking and degradation of M1 and M2 mAChRs. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2222043 Wed, 03 Dec 2008 05:00:00 +0100 2222043 http://www.medworm.com/index.php?rid=2010777&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F20 Conclusions: These findings indicate that ubiquitination of beta-arrestin has a distinct role in the differential trafficking and degradation of M1 and M2 mAChRs. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2010777 Wed, 03 Dec 2008 05:00:00 +0100 2010777 http://www.medworm.com/index.php?rid=2004796&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F18 Conclusions: Cell cycle arrest in response to metformin requires CDK inhibitors in addition to AMPK activation and cyclin D1 downregulation. This is of interest because many cancers are associated with loss or downregulation of CDK inhibitors and the results may be relevant to the development of anti-tumor reagents that target the AMPK pathway (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=2004796 Mon, 01 Dec 2008 05:00:00 +0100 2004796 http://www.medworm.com/index.php?rid=1494134&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F10 Conclusion: This study demonstrates that Wnt-3a treatment activates the Wnt signalling pathway and interferes with in vitro differentiation of mammary co-cultures to β-casein production in response to lactogenic hormones. Similarly, in another measure of differentiation, following Wnt-3a treatment mammary epithelial cells could be shown to up-regulate the cyclin D1 and connexin-43 genes while phenotypically they show increased transepithelial resistance across the cell monolayer. All these behavioural changes can be blocked in mammary epithelial cells expressing SFRP-4. Thus, our data illustrate in an in vitro model a mechanism by which SFRP-4 can modulate a differentiation response to Wnt-3a. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1494134 Fri, 02 May 2008 04:00:00 +0100 1494134 http://www.medworm.com/index.php?rid=1399011&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F9 Suspension cultures of Catharanthus roseus were used to evaluate ultraviolet-B (UV-B) treatment as an abiotic elicitor of secondary metabolites. A dispersed cell suspension culture from C. roseus leaves in late exponential phase and stationary phase were irradiated with UV-B for 5 min. The stationary phase cultures were more responsive to UV-B irradiation than late exponential phase cultures. Catharanthine and vindoline increased 3-fold and 12-fold, respectively on treatment with a 5-min UV-B irradiation. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1399011 Fri, 25 Apr 2008 04:00:00 +0100 1399011 http://www.medworm.com/index.php?rid=1340077&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F8 Conclusions: Taken together our results define the tyrosine residues of KDR that are regulated by SHP-1 and also elucidates a novel feed back loop where SHP-1 is activated upon VEGF treatment through c-Src and controls KDR induced DNA synthesis, eventually leading to controlled angiogenesis. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1340077 Mon, 31 Mar 2008 04:00:00 +0100 1340077 http://www.medworm.com/index.php?rid=1316529&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F7 Conclusion: Inhibition of PI3K/AKT and MEK/ERK pathways act synergistically to regulate antiangiogenic effects of EGCG through activation of FOXO transcription factors. The activation of FOXO transcription factors through inhibition of these two pathways may have physiological significance in management of diabetic retinopathy, rheumatoid arthritis, psoriasis, cardiovascular diseases, and cancer. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1316529 Thu, 20 Mar 2008 04:00:00 +0100 1316529 http://www.medworm.com/index.php?rid=1261451&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F6 Conclusions: Our results suggest that the dimerization of the alpha-1D-AR with other ARs does not alter the cellular expression or functional response characteristics of the alpha-1D-AR. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1261451 Wed, 27 Feb 2008 05:00:00 +0100 1261451 http://www.medworm.com/index.php?rid=1259740&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F5 Conclusions: Analyses of multiple poses of conformationally-restricted anandamide analogs permitted identification of favored amino acid interactions within the CB1 receptor binding pocket. A ligand possessing both high affinity and cannabinoid agonist efficacy was able to interact with both polar and hydrophobic interaction sites utilized by the potent and efficacious non-classical cannabinoid CP55940. In contrast, other analogs characterized by reduced affinity or efficacy exhibited less favorable interactions with those key residues. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1259740 Tue, 26 Feb 2008 05:00:00 +0100 1259740 http://www.medworm.com/index.php?rid=1244878&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F4 Wiskott Aldrich Syndrome protein (WASP) has a unique regulatory role in sealing ring formation and bone resorption in osteoclasts. Here, using the TAT-transduction method, we show the possible role of WASP domain(s) in sealing ring formation and bone resorption. Transduction of TAT-fused full-length WASP peptide induced Arp2/3 complex formation, F-actin content, sealing ring formation and bone resorption. Transduction of WASP peptides containing basic, verpolin-central, pTyr294, and proline-rich regions inhibited the processes listed above at various levels. The ability to resorb bone by WASP peptides containing basic, verpolin-central, and proline-rich regions was reduced and the resorbed area matched the size of the sealing ring. However, osteoclasts transduced with WASP peptide containi... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1244878 Wed, 20 Feb 2008 05:00:00 +0100 1244878 http://www.medworm.com/index.php?rid=1224958&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F3%2F1%2F3 Cutaneous melanoma is often resistant to chemo- and radiotherapy. This resistance has recently been demonstrated to be due, at least in part, to high activating transcription factor 2 (ATF 2) activity in these tumors. In concordance with these reports, we found that B16 mouse melanoma cells had higher levels of ATF-2 than immortalized, but non-malignant mouse melanocytes. In addition, the melanoma cells had a much higher amount of phosphorylated (active) ATF-2 than the immortalized melanocytes. In the course of determining how retinoic acid (RA) stimulates activating protein-1 (AP-1) activity in B16 melanoma, we discovered that this retinoid decreased the phosphorylation of ATF-2. It appears that this effect is mediated through p38 MAPK, because RA decreased p38 phosphorylation (activation... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=1224958 Tue, 12 Feb 2008 05:00:00 +0100 1224958 http://www.medworm.com/index.php?rid=991668&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F13 Heterotrimeric G proteins are ubiquitous signaling partners of seven transmembrane-domain G-protein-coupled receptors (GPCRs), the largest (and most important pharmacologically) receptor family in mammals. A number of scaffolding proteins have been identified that regulate various facets of GPCR signaling. In this review, we summarize current knowledge concerning those scaffolding proteins that are known to directly bind heterotrimeric G proteins, and discuss the composition of the protein complexes they assemble and their effects on signal transduction. Emerging evidence about possible ways of regulation of activity of these scaffolding proteins is also discussed. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=991668 Tue, 30 Oct 2007 04:00:00 +0100 991668 http://www.medworm.com/index.php?rid=978901&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F11 Conclusions: This study provides a detailed biochemical analysis of signaling elements key to Wnt3a regulation of the canonical pathway. We quantify, for the first time, the Wnt-dependent regulation of cellular abundance and intracellular trafficking of these signaling molecules. In contrast, we observe little effect of Wnt3a stimulation on the level of protein-protein interactions among these constituents of Axin-based complexes themselves. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=978901 Thu, 25 Oct 2007 04:00:00 +0100 978901 http://www.medworm.com/index.php?rid=978900&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F12 Conclusions: In current study, we showed new roles of phosphoprotein phosphatase-2A in Wnt/beta-catenin signaling pathway: effect on protein expression, effect on protein trafficking, retention of molecules in subcellular compartments, and regulation of enzymatic activity of several key players. Docking of phosphoprotein phosphatase-2A by Dishevelled-2 suppresses phosphatase activity and explains in part the central role of this phosphatase in the counterregulation of the Wnt/beta-catenin signaling pathway. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=978900 Thu, 25 Oct 2007 04:00:00 +0100 978900 http://www.medworm.com/index.php?rid=927636&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F10 Conclusion: The ability of curcumin to inhibit capillary tube formation and cell migration, and enhance the therapeutic potential of TRAIL suggests that curcumin alone or in combination with TRAIL can be used for prostate cancer prevention and/or therapy. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=927636 Thu, 04 Oct 2007 04:00:00 +0100 927636 http://www.medworm.com/index.php?rid=895449&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F9 Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=895449 Tue, 25 Sep 2007 00:29:06 +0100 895449 http://www.medworm.com/index.php?rid=887954&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F8 Conclusions: Ligand activated sigma-1 receptors translocate into FAC from a pool of receptors stored in ER lipid rafts presumably for inhibition of Kv1.4 channels. Stabilization of actin filaments is likely to be important for targeting sigma-1 receptors to Focal Adhesion Contacts in CHO-K1 cells. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=887954 Thu, 20 Sep 2007 04:00:00 +0100 887954 http://www.medworm.com/index.php?rid=736184&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F6 Conclusion: In conclusion, we have demonstrated that the Wnt signaling pathway specifically regulates one out of three CLU mRNA variants via TCF1. This CLU transcript is shorter at the 5' end than reported by the RefSeq database, and produces the intracellular 60 kDa CLU protein isoform which is secreted as a ~80 kDa protein after post-translational processing. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=736184 Mon, 16 Jul 2007 04:00:00 +0100 736184 http://www.medworm.com/index.php?rid=716325&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F4 Conclusions: Our results demonstrate that the RGMs play a significant role in BMP signaling and reveal that these molecules cannot functionally compensate for one another. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=716325 Thu, 05 Jul 2007 04:00:00 +0100 716325 http://www.medworm.com/index.php?rid=716324&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F5 Conclusion: Here, we demonstrate that human PNRC stimulates RNA pol III transcription through its interaction with the subunit RPC39 of RNA pol III. PNRC is a unique coactivator that has profound effects on many aspects of cellular function by directly influencing both RNA pol II- and RNA pol III-dependent transcription. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=716324 Thu, 05 Jul 2007 04:00:00 +0100 716324 http://www.medworm.com/index.php?rid=440483&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F2 G protein-coupled receptors are key regulators of cellular communication, mediating the efficient coordination of a cell's responses to extracellular stimuli. When stimulated these receptors modulate the activity of a wide range of intracellular signalling pathways that facilitate the ordered development, growth and reproduction of the organism. There is now a growing body of evidence examining the mechanisms by which G protein-coupled receptors are able to regulate the expression, activity, localization and stability of cell cycle regulatory proteins that either promote or inhibit the initiation of DNA synthesis. In this review, we will detail the intracellular pathways that mediate the G protein-coupled receptor regulation of cellular proliferation, specifically the progression from the ... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=440483 Mon, 26 Feb 2007 05:00:00 +0100 440483 http://www.medworm.com/index.php?rid=400168&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F2%2F1%2F1 We have identified human ArhGAP9 as a novel MAP kinase docking protein that interacts with Erk2 and p38alpha through complementarily charged residues in the WW domain of ArhGAP9 and the CD domains of Erk2 and p38alpha. This interaction sequesters the MAP kinases in their inactive states through displacement of MAP kinase kinases targeting the same sites. While over-expression of wild type ArhGAP9 caused MAP kinase activation by the epidermal growth factor receptor (EGFR) to be suppressed and preserved the actin stress fibres in quiescent Swiss 3T3 fibroblasts, over-expression of an ArhGAP9 mutant defective in MAP kinase binding restored EGFR-induced MAP kinase activation and resulted in significant disruption of the stress fibres, consistent with the role of Erk activation in disassembly o... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=400168 Tue, 06 Feb 2007 07:00:00 +0100 400168 http://www.medworm.com/index.php?rid=362779&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F8 The strict spatio-temporal control of Rho GTPases is critical for many cellular functions, including cell motility, contractility, and growth. In this regard, the prototypical Rho family GTPases, Rho, Rac, and Cdc42 regulate the activity of each other by a still poorly understood mechanism. Indeed, we found that constitutively active forms of Rac inhibit stress fiber formation and Rho stimulation by thrombin. Surprisingly, a mutant of Rac that is unable to activate Pak1 failed to inhibit thrombin signaling to Rho. To explore the underlying mechanism, we investigated whether Pak1 could regulate guanine nucleotide exchange factors (GEFs) for Rho. We found that Pak1 associates with P115-RhoGEF but not with PDZ-RhoGEF or LARG, and knock down experiments revealed that P115-RhoGEF plays a major ... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362779 Wed, 06 Dec 2006 07:00:00 +0100 362779 http://www.medworm.com/index.php?rid=362782&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F5 Conclusion: Our results suggest that physiological levels of E2 may act to sequester DAT in intracellular compartments where the transporter's second extramembrane loop is inaccessible (inside vesicles) and that rapid estrogenic actions on this differentiated neuronal cell type may be regulated via membrane ERs of several types. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362782 Tue, 05 Dec 2006 07:00:00 +0100 362782 http://www.medworm.com/index.php?rid=362781&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F6 Seven members of the Mix family of paired-type homeoproteins regulate mesoderm/endoderm differentiation in amphibians. In mammals, the MIXL1 (Mix. 1 homeobox [Xenopus laevis]-like gene 1) gene is the sole representative of this family. Unlike the amphibian Mix genes that encode an open reading frame of >300 amino acids, mammalian MIXL1 encodes a smaller protein (~230aa). However, mammalian MIXL1 contains a unique proline-rich domain (PRD) with a potential to interact with signal transducing Src homolgy 3 (SH3) domains. Notably, human MIXL1 also contains a unique tyrosine residue Tyr20 that is amino-terminal to the PRD. Here we report that mammalian MIXL1 protein is phosphorylated at Tyr20 and the phosphorylation is dramatically reduced in the absence of PRD. Our findings are consistent wit... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362781 Tue, 05 Dec 2006 07:00:00 +0100 362781 http://www.medworm.com/index.php?rid=362780&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F7 Conclusion: In summary, this study demonstrates that agonist-promoted internalization of M2 mAChRs is β-arrestin- and clathrin-dependent, and that the receptor stably co-localizes with β-arrestin in early endosomal vesicles. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362780 Tue, 05 Dec 2006 07:00:00 +0100 362780 http://www.medworm.com/index.php?rid=362784&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F1 Molecular signaling is an exponentially growing field that encompasses different molecular aspects of cell signaling underlying normal and pathological conditions. This area also focuses on defining the genetic and epigenetic changes that modulate the signaling properties of cells and the resultant physiological as well as pathological conditions. Therefore, rapid publication of results from these endeavors and, more importantly, free access to such publications can truly accelerate the progress in this field leading to the development of novel targeted drugs. With this goal in mind, Journal of Molecular Signaling, a journal fully devoted to open access publishing of rigorously peer-reviewed quality manuscripts in the molecular signaling area of research, is being launched. The focus, sign... Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362784 Fri, 10 Nov 2006 07:00:00 +0100 362784 http://www.medworm.com/index.php?rid=362783&cid=s_34078_67_f&fid=34078&url=http%3A%2F%2Fwww.jmolecularsignaling.com%2Fcontent%2F1%2F1%2F3 Conclusion: Based on the opposing effects of taxol and the Gαi1 protein on the microtubule dynamic instability (taxol suppresses microtubule dynamic instability whilst the Gαi1 protein inhibits the suppression) our results indicate the operation of a novel pathway that would enable the cells to escape the cytotoxic effects of taxol. (Source: Journal of Molecular Signaling) Journal of Molecular Signaling journals http://www.medworm.com/rss/comments.php?id=362783 Fri, 10 Nov 2006 07:00:00 +0100 362783