MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Journal of Nanobiotechnology' source. Tue, 07 Feb 2012 10:30:11 +0100 http://www.medworm.com/index.php?rid=5665317&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F8 Conclusion: This work gives entry into a new class of DNA/gold nanoparticles hybrid materials which might have optical property that can be controlled for application in diagnostics. We note that it should be possible to extend this strategy easily for developing new types of DNA biosensor for point of care detection. The salient feature of this approach includes low-cost, robust reagents and simple colorimetric detection of pathogen. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5665317 Mon, 06 Feb 2012 05:00:00 +0100 5665317 http://www.medworm.com/index.php?rid=5658145&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F7 Conclusion: Amino-acid substitution in the spacer of gemini surfactant did not alter the cellular uptake pathway, showing similar pattern to the unsubstituted parent gemini surfactant. Glycyl-lysine substitution in the gemini spacer improved buffering capacity and imparted a pH-dependent increase of particle size. This property conferred to the P/12-7NGK-12/L nanoparticles the ability to escape efficiently from clathrin-mediated endosomes. Balanced binding properties (protection and release) of the 12-7NGK-12 in the presence of polyanions could contribute to the facile release of the nanoparticles internalized via caveolae-mediated uptake. A more efficient endosomal escape of the P/12-7NGK-12/L nanoparticles lead to higher gene expression compared to the parent gemini surfactant. (Source: ... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5658145 Wed, 01 Feb 2012 05:00:00 +0100 5658145 http://www.medworm.com/index.php?rid=5658146&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F6 Conclusion: We have established that metal/metal alloy coated silicon dioxide nanosprings can be used as a biomimetic material paralleling the morphology and biology of osteogenic extracellular matrix. The coated nanosprings enhance normal human osteoblasts cellular behaviors needed for improving osseointegration of orthopedic materials. Thus, metal-coated nanosprings represent a novel biomaterial that could be exploited for improving success rates of orthopedic implant procedures. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5658146 Fri, 27 Jan 2012 05:00:00 +0100 5658146 http://www.medworm.com/index.php?rid=5626472&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F5 Conclusions: The induced histological alterations might be an indication of injured hepatocytes due to GNPs toxicity that became unable to deal with the accumulated residues resulting from metabolic and structural disturbances caused by these NPs. These alterations were size-dependent with smaller ones induced the most effects and related with time exposure of GNPs. The appearance of hepatocytes cytoplasmic degeneration and nuclear destruction may suggest that GNPs interact with proteins and enzymes of the hepatic tissue interfering with the antioxidant defense mechanism and leading to reactive oxygen species (ROS) generation which in turn may induce stress in the hepatocytes to undergo atrophy and necrosis. More histomorphologcal, histochemical and ultrastrucural investigations are needed... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5626472 Wed, 25 Jan 2012 05:00:00 +0100 5626472 http://www.medworm.com/index.php?rid=5618454&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F4 Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days). (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5618454 Fri, 20 Jan 2012 05:00:00 +0100 5618454 http://www.medworm.com/index.php?rid=5570753&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F1 In this report we demonstrate that the human plasma membrane protein DARC can be used as an artificial anchor molecule in cell surface engineering applications. We constructed the fusion protein SNAP-tag-DARC, consisting of DARC and the self-labeling protein tag SNAP-tag (Covalys). The SNAP-tag served as an example for a molecular-technological developed protein that is artificially attached to the extracellular side of the plasma membrane through our DARC-anchor. SnapTag should serve as an example for any extracellular entity and was easy to detect by a commercial detection system. The synthesis of SNAP-tag-DARC, its correct incorporation into the cell membrane and the functionality of the SNAP-tag were verified by RT-PCR, Western blotting and confocal fluorescence microscopy and showed t... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5570753 Thu, 05 Jan 2012 05:00:00 +0100 5570753 http://www.medworm.com/index.php?rid=5570752&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F2 Conclusions: Based upon these findings, we propose that the bioactive waterborne polyurethane nanomicelles can be used as an effective nanocarrier for delivery of anticancer chemotherapies such as paclitaxel. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5570752 Thu, 05 Jan 2012 05:00:00 +0100 5570752 http://www.medworm.com/index.php?rid=5570751&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F10%2F1%2F3 Conclusions: A rapid, mild method for synthesizing Fe3O4-gold nanoparticles using chitosan was investigated. A magnetic core-shell-chitosan nanocomposite, including both the supermagnetic properties of iron oxide and the optical characteristics of colloidal gold nanoparticles, was synthesized. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5570751 Thu, 05 Jan 2012 05:00:00 +0100 5570751 http://www.medworm.com/index.php?rid=5557544&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F59 Conclusions: This study demonstrates the ability to construct fully electro-active cyt c multilayer assemblies by using carboxy-modified silica nanoparticles. Thus it can be shown that functional, artificial systems can be build up following natural examples of protein arrangements. The absence of any conductive properties in the second building block clearly demonstrates that mechanisms for electron transfer through such protein multilayer assemblies is based on interprotein electron exchange, rather than on wiring of the protein to the electrode.The construction strategy of this multilayer system provides a new controllable route to immobilize proteins in multiple layers featuring direct electrochemistry without mediating shuttle molecules and controlling the electro-active amount by the... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5557544 Fri, 30 Dec 2011 05:00:00 +0100 5557544 http://www.medworm.com/index.php?rid=5522162&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F58 Conclusion: Our results suggest that aminoglycosides may serve as branched solubility enhancing linkers for drug conjugation that also provide for a better safety profile of the targeted nanomedicine. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5522162 Tue, 20 Dec 2011 05:00:00 +0100 5522162 http://www.medworm.com/index.php?rid=5485017&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F57 Conclusions: Maskless array synthesis is an efficient and versatile method for synthesizing high density arrays of long oligonucleotides for hybridization- and other molecular binding-based experiments. For applications requiring high sequence purity, such as gene assembly, diffraction and flare remain significant obstacles, but can be significantly reduced with straightforward experimental strategies. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5485017 Thu, 08 Dec 2011 05:00:00 +0100 5485017 http://www.medworm.com/index.php?rid=5485018&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F56 Conclusion: Present study provides the indication of involvement of molecular machinery and various cellular proteins in the biosynthesis of silver nanoparticles. In this report, the study is mainly focused towards understanding the role of diverse cellular protein in the synthesis and capping of silver nanoparticles using C. reinhardtii as a model system. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5485018 Wed, 07 Dec 2011 05:00:00 +0100 5485018 http://www.medworm.com/index.php?rid=5455438&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F55 Biodegradable nanoparticles (NPs) are gaining increased attention for their ability to serve as a viable carrier for site specific delivery of vaccines, genes, drugs and other biomolecules in the body. They offer enhanced biocompatibility, superior drug/vaccine encapsulation, and convenient release profiles for a number of drugs, vaccines and biomolecules to be used in a variety of applications in the field of medicine. In this manuscript, the methods of preparation of biodegradable NPs, different factors affecting optimal drug encapsulation, factors affecting drug release rates, various surface modifications of nanoparticles to enhance in-vivo circulation, distribution and multimodal functionalities along with the specific applications such as tumor targeting, oral delivery, and delivery ... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5455438 Mon, 28 Nov 2011 05:00:00 +0100 5455438 http://www.medworm.com/index.php?rid=5420097&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F54 Conclusions: The production of DNA-nanoarrays is a promising task for nanobiotechnology. The describedmethod allows convenient and low cost preparation of nanoarrays. PNA can be used for complexfunctionalization purposes as well as a structural element. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5420097 Fri, 18 Nov 2011 05:00:00 +0100 5420097 http://www.medworm.com/index.php?rid=5420099&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F52 Conclusions: The assay combining efficient magnetic separation and MALDI-MS readout offers a rapid and sensitive method for virus screening. Direct on-MNP detection by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provided high sensitivity (~103 EID50 per mL) and a timely diagnosis within one hour. The magnetic nanoparticles encapsulated with monoclonal antibodies could be used as a specific probe to distinguish different subtypes of influenza. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5420099 Wed, 16 Nov 2011 05:00:00 +0100 5420099 http://www.medworm.com/index.php?rid=5420098&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F53 Conclusion: These increases in the transformation capacity mediated by MWCNT, in meningococci, indicate the interaction of these particles with the virulence acquisition of these bacteria, as well as with the increase in the vaccination escape process. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5420098 Wed, 16 Nov 2011 05:00:00 +0100 5420098 http://www.medworm.com/index.php?rid=5405283&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F51 After publication of this work [Yu et al, 2011: Journal of Nanobiotechnology, 9, 7], we found an error in the figure legend for Figure two. In Figure twoA, GPC chromatograms of the synthesized co-polymers were shown, but the completed cPEG-PPS product contains some residual PPS-COOH, not H2N-PEG-COOH as originally described in the figure legend. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5405283 Mon, 14 Nov 2011 05:00:00 +0100 5405283 http://www.medworm.com/index.php?rid=5398545&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F49 In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n=524) of the total genes (n=5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergen... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5398545 Thu, 10 Nov 2011 05:00:00 +0100 5398545 http://www.medworm.com/index.php?rid=5398544&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F50 Conclusions: The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5398544 Thu, 10 Nov 2011 05:00:00 +0100 5398544 http://www.medworm.com/index.php?rid=5345157&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F48 Conclusion: A QD assay was developed in this study that provides a direct method for quantifying ligand attachment sites of avidin covalently bound to surfaces. The strong fluorescence signal that is a fundamental characteristic of QDs allows for the measurement of small changes in the amount of these particles in solution or attached to surfaces. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5345157 Mon, 24 Oct 2011 04:00:00 +0100 5345157 http://www.medworm.com/index.php?rid=5333205&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F47 Conclusions: This study demonstrated that transfection efficiency of DNA-expression-plasmids was significantly improved by the addition of AuNPs. In some combinations the respective cytotoxicity was increased depending on the type of the applied AuNPs and the transfected DNA construct. Consequently, our results indicate that for routine use of these AuNPs the specific nanoparticle formulation and DNA construct combination has to be considered. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5333205 Thu, 20 Oct 2011 04:00:00 +0100 5333205 http://www.medworm.com/index.php?rid=5295115&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F46 An electrochemical sensor for p-aminophenyl phosphate (pAPP) is reported. It is based on the electrochemical conversion of 4-aminophenol (4AP) at a quantum dot (QD) modified electrode under illumination. Without illumination no electron transfer and thus no oxidation of 4AP can occur. pAPP as substrate is converted by the enzyme alkaline phosphatase (ALP) to generate 4AP as a product. The QDs are coupled via 1, 4 benzene dithiol (BDT) linkage to the surface of a gold electrode and thus allow potential-controlled photocurrent generation. The photocurrent is modified by the enzyme reaction providing access to the substrate detection. In order to develop a photobioelectrochemical sensor the enzyme is immobilized on top of the photo-switchable layer of the QDs. Immobilization of ALP is require... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5295115 Fri, 07 Oct 2011 04:00:00 +0100 5295115 http://www.medworm.com/index.php?rid=5271414&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F45 Conclusions: These measurements, combined, demonstrated that Pluronic-stabilized SWCNTs only enter cells via energy-dependent endocytosis, and association of SWCNTs to membrane likely increased uptake. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5271414 Fri, 30 Sep 2011 04:00:00 +0100 5271414 http://www.medworm.com/index.php?rid=5271415&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F44 Conclusion: The results of irritation potential studies and in vivo assessments indicate that this nanoemulsion has potential to be a useful tool to treat skin diseases, such as atopic dermatitis and psoriasis. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5271415 Wed, 28 Sep 2011 04:00:00 +0100 5271415 http://www.medworm.com/index.php?rid=5258474&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F43 This study revealed completely would eliminate the use of expensive drug for cancer treatment. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5258474 Mon, 26 Sep 2011 04:00:00 +0100 5258474 http://www.medworm.com/index.php?rid=5247967&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F41 Conclusions: This study demonstrates the influence of formulation on the physico-chemical properties of each nano-emulsion obtained by the mixture design. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5247967 Wed, 21 Sep 2011 04:00:00 +0100 5247967 http://www.medworm.com/index.php?rid=5235753&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F39 Conclusions: The results indicate that the convoluted FPs of the quasi-in vivo cell network assay includes both of the repolarization data and the conductance abnormality of cardiomyocyte networks has the strong potential to prediction lethal arrhythmia. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5235753 Mon, 19 Sep 2011 04:00:00 +0100 5235753 http://www.medworm.com/index.php?rid=5235752&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F40 Conclusions: These findings demonstrate that nanoparticle-based delivery of siRNAs directed at critical targets such as DCAMKL-1 may provide a novel approach to treat cancer through the regulation of endogenous miRNAs. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5235752 Mon, 19 Sep 2011 04:00:00 +0100 5235752 http://www.medworm.com/index.php?rid=5221150&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F37 Conclusions: Multiple target labeling is a unique feature of the intein based methodology which sets it apart from existing tagging methodologies in that, given the large number of characterized split inteins, the number of individual targets that can be simultaneously tagged is only limited by the number of QDs that can be spectrally distinguished within the cell. Therefore, the intein-mediated approach for simultaneous, in vivo, site-specific (N- and C-terminus) conjugation of Quantum Dots to multiple protein targets opens up new possibilities for bioimaging applications and offers an effective system to target QDs and other nanostructures to intracellular compartments as well as specific molecular complexes. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5221150 Thu, 15 Sep 2011 04:00:00 +0100 5221150 http://www.medworm.com/index.php?rid=5192052&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F36 Conclusions: Both IGF-1 radioimmuno and CNT-FET assays gave comparable results. Indeed, the CNT-FET assay was simpler and faster than the radioimmunoassay. Additionally, the low serum sample required by CNT-FETs can be especially advantageous for studies constricted by limited amount of human clinical samples and for mouse studies, since animals often need to be sacrificed to obtain enough serum for biomarker evaluation. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5192052 Thu, 01 Sep 2011 23:00:00 +0100 5192052 http://www.medworm.com/index.php?rid=5181560&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F35 Conclusions: We propose an optimized methodology for the preparation of homogeneous TI-complexes containing only tubular particles, which would achieve reproducible immunization results. We suggest that the elaborated TI-complexes apply as a universal delivery system for different subunit antigens within anti-infectious vaccines and enhance their economic efficacy and safety. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5181560 Thu, 01 Sep 2011 23:00:00 +0100 5181560 http://www.medworm.com/index.php?rid=5158698&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F34 Conclusion: Altogether the study suggests that there is metal nanoparticle - bacteria interaction at the cellular level that can be utilized for beneficial biological application but significantly it also posses potential to produce ecotoxicity, challenging the ecofriendly nature of nanoparticles. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5158698 Mon, 22 Aug 2011 23:00:00 +0100 5158698 http://www.medworm.com/index.php?rid=5158699&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F33 Conclusion: In this study using a simple "semi rational" approach the FhuA Delta1-159 diameter was enlarged. By combining the actual results with the previous ones on the FhuADelta1-159 lengthening a new set of synthetic nanochannels with desired lengths and diameters can be produced, broadening the FhuA Delta1-159 applications. As large scale protein productionis possible our approach can give a contribution to nanochannel industrial applications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5158699 Thu, 18 Aug 2011 23:00:00 +0100 5158699 http://www.medworm.com/index.php?rid=5118497&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F32 ${item.shortDescription} (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5118497 Wed, 10 Aug 2011 23:00:00 +0100 5118497 http://www.medworm.com/index.php?rid=5107365&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F31 ${item.shortDescription} (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5107365 Sun, 07 Aug 2011 23:00:00 +0100 5107365 http://www.medworm.com/index.php?rid=5095833&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F30 ${item.shortDescription} (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5095833 Tue, 02 Aug 2011 23:00:00 +0100 5095833 http://www.medworm.com/index.php?rid=5077490&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F29 ${item.shortDescription} (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5077490 Thu, 28 Jul 2011 23:00:00 +0100 5077490 http://www.medworm.com/index.php?rid=5062082&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F28 ${item.shortDescription} (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=5062082 Sun, 24 Jul 2011 23:00:00 +0100 5062082 http://www.medworm.com/index.php?rid=4975680&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F27 Conclusions: Atomic force microscope can be easily used to visualize and to manipulate biological material with high resolution and accuracy. The fluorescence in situ hybridization (FISH) performed with the DOP-PCR products as test probes has been tested succesfully in avian microchromosomes and interphasic nuclei. Chromosome nanolithography, with a resolution beyond the resolution limit of light microscopy, could be useful to the construction of chromosome band libraries and to the molecular cytogenetic mapping related to the investigation of genetic diseases. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4975680 Mon, 27 Jun 2011 23:00:00 +0100 4975680 http://www.medworm.com/index.php?rid=4952614&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F26 Conclusions: To the best of our knowledge, immobilization of a protein by the method presented here, with the resulting high enzymatic activity, has never been reported. There are many potential applications for selective localization of active proteins at patterned surfaces, for example, bioMEMS (MEMS - Micro-Electro-Mechanical Systems. Due to the success of the method, presented here, it was decided to continue a research project of a biosensor by transferring it to a high aspect ratio platform - nanotubes. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4952614 Sun, 19 Jun 2011 23:00:00 +0100 4952614 http://www.medworm.com/index.php?rid=4902251&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F25 Conclusions: HAPIscreen, an Alphascreen(R)-based methodology for the identification of aptamers is faster and less biased than current procedures based on sequence comparison of selected oligonucleotides and sampling binding properties of few individuals. Moreover this methodology allows for screening larger number of candidates. Used here for selecting anti-premiR aptamers, HAPIscreen can be adapted to any type of tagged target and is fully amenable to automation. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4902251 Thu, 02 Jun 2011 23:00:00 +0100 4902251 http://www.medworm.com/index.php?rid=4871952&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F24 Conclusions: The method presented in this paper allows us to obtain information about the radial deformation of the hydrogel matrix due to cell growth, and the consequent modification of the pore size distribution pattern surrounding the cells, which are extremely important for a wide spectrum of biotechnological, pharmaceutical and biomedical applications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4871952 Thu, 26 May 2011 23:00:00 +0100 4871952 http://www.medworm.com/index.php?rid=4862377&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F23 This study is aimed to develop one kind of multifunctional nanoprobes for in vivo targeted magnetofluorescent imaging of gastric cancer. Methods: BRCAA1 monoclonal antibody was prepared, was used as first antibody to stain 50 pairs of specimens of gastric cancer and control normal mucous tissues, and conjugated with fluorescent magnetic nanoparticles with 50 nm in diameter, the resultant BRCAA1-conjugated fluorescent magnetic nanoprobes were characterized by transmission electron microscopy and photoluminescence spectrometry, as-prepared nanoprobes were incubated with gastric cancer MGC803 cells, and were injected into mice model loaded with gastric cancer of 5mm in diameter via tail vein, and then were imaged by fluorescence optical imaging and magnetic resonance imaging, their biodistrib... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4862377 Tue, 24 May 2011 23:00:00 +0100 4862377 http://www.medworm.com/index.php?rid=4862378&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F22 Conclusions: VLPs assembled from MS2 single-chain dimer coat proteins with peptide insertions in one of their AB-loops are somewhat less stable than the wild-type particle, but still resist heating up to about 50degreesC. Because they possess disulfide cross-links, PP7-derived VLPs provide an alternate platform with even higher stability. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4862378 Mon, 23 May 2011 23:00:00 +0100 4862378 http://www.medworm.com/index.php?rid=4852757&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F20 Conclusion: The molecular aberrations observed from this study may become new biomarkers for prostate cancer detection. The nanoparticle immunoassay reported here can be used as a convenient and general tool to screen and analyze serum proteins and to discover new biomarkers associated with cancer and other human diseases. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4852757 Sun, 22 May 2011 23:00:00 +0100 4852757 http://www.medworm.com/index.php?rid=4852756&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F21 Conclusions: The results indicated that fibroblasts can connect cardiomyocytes electrically but do not significantly enhance and contribute to beating interval stability and synchronization. This might also mean that an increase in the number of fibroblasts in heart tissue reduces the cardiomyocyte 'community effect', which enhances synchronization and stability of their beating rhythms. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4852756 Sun, 22 May 2011 23:00:00 +0100 4852756 http://www.medworm.com/index.php?rid=4828700&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F19 Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4828700 Mon, 16 May 2011 23:00:00 +0100 4828700 http://www.medworm.com/index.php?rid=4828701&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F18 Conclusion: The presented approach of vapor-phase coating of solid-state structures is important and applicable in many areas of bio-nano interface development. The exposure of coatings to the solutions of different pH showed the stability of the coatings in chemical surroundings. The organic nanocoating of films can be used in bio-implants and many medical devices. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4828701 Fri, 13 May 2011 23:00:00 +0100 4828701 http://www.medworm.com/index.php?rid=4813106&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F17 Conclusion: The characterization and definition of the examined gold and silica NM can benefit from application of conventional bright field electron tomography: the NM can be visualized in 3D, while surface features and the VSSA can be measured. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4813106 Tue, 10 May 2011 23:00:00 +0100 4813106 http://www.medworm.com/index.php?rid=4813107&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F16 Conclusions: Factors affecting the fluorescence of a fluorophore placed near a GNP are the GNP size, coating material on GNP, wavelengths of the incident light and emitted light and intrinsic quantum yield of the fluorophore. Experimentally, we were able to quench and enhance the fluorescence of Cypate, by changing the distance between the fluorophore and GNP. This ability of artificially controlling fluorescence can be beneficially used in developing contrast agents for highly sensitive and specific optical sensing and imaging. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4813107 Mon, 09 May 2011 23:00:00 +0100 4813107 http://www.medworm.com/index.php?rid=4762689&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F15 Conclusion: Bone marrow stem cells were isolated, characterized and transplanted in mice model. Biodegradable biopolymeric nanoparticles were prepared with the pleotrophic protein molecule and it worked well for the differentiation of stem cells, especially mesenchymal phenotypic cells. Transplantation of bone marrow MSC in combination with HGF-CNP could be an ideal approach for the treatment of liver cirrhosis. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4762689 Wed, 27 Apr 2011 23:00:00 +0100 4762689 http://www.medworm.com/index.php?rid=4696542&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F14 Conclusions: We demonstrated the feasibility and effectiveness of our method, proving that it is accurate enough to measure the nanoparticle uptake differences between cells as well as the sizes of the formed nanoparticle clusters. The differences between 2D and 3D cultures and 2D and 3D images stress the importance of the 3D analysis which is made possible by our approach. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4696542 Sat, 09 Apr 2011 23:00:00 +0100 4696542 http://www.medworm.com/index.php?rid=4693179&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F13 Conclusion: Our experiments have demonstrated that beta-ME visibly enhances the emission intensity. No deleterious effects to the cells were witnessed upon co-incubation with beta-ME alone and no increases in background fluorescence were recorded. These results should present an interest for further development of in vitro biological imaging techniques. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4693179 Thu, 07 Apr 2011 23:00:00 +0100 4693179 http://www.medworm.com/index.php?rid=4684108&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F12 The methods of the published manuscript [Jasmin et al, Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging. Journal of Nanobiotechnology 2011, 9:4] state that an incorrect concentration of Feridex was used (25 ug/ml). Instead, the methods should state that a concentration of 50 ug/ml Feridex was used. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4684108 Tue, 05 Apr 2011 23:00:00 +0100 4684108 http://www.medworm.com/index.php?rid=4642497&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F10 Conclusions: The physical and chemical properties of the nanoparticle produced by variation in the acid side chains that modify size and shape of engineered nanostructure and their interplay with host cell physiology might be the major criteria for their differential uptake to different internal organs. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4642497 Sun, 27 Mar 2011 23:00:00 +0100 4642497 http://www.medworm.com/index.php?rid=4642496&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F11 Background: Rhodium (II) citrate has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascite tumor. Although toxic to normal cells, its lower toxicity when compared to carboxylate analogues of rhodium (II) indicates rhodium (II) citrate as a promising agent for chemotherapy. Nevertheless, few studies have been performed to explore this potential. Superparamagnetic particles of iron oxide (SPIOs) represent an attractive platform as carriers in drug delivery systems (DDS) because they can present greater specificity to tumor cells than normal cells. Thus, the association between rhodium (II) citrate and SPIOs can represent a strategy to enhance the former's therapeutic action. In this work, we report the cytotoxicity of free rhodium (II) citrate and rhodium (II) citrate-l... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4642496 Sun, 27 Mar 2011 23:00:00 +0100 4642496 http://www.medworm.com/index.php?rid=4605723&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F8 Conclusion: Using a simple "semi rational" approach the protein's hydrophobic transmembrane region was increased by 1 nm, leading to a predicted lower hydrophobic mismatch between the protein and polymer membrane, minimizing the insertion energy penalty. The strategy of adding amino acids to the FhuA delta1-159 Ext hydrophobic part can be further expanded to increase the protein's hydrophobicity, promoting the efficient embedding into thicker/ more hydrophobic block copolymer membranes. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4605723 Thu, 17 Mar 2011 00:00:00 +0100 4605723 http://www.medworm.com/index.php?rid=4527684&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F7 Conclusions: This work demonstrates proof of concept of an enzymatically-actuatable and thermoresponsive system for dynamic biosensing applications. The platform exhibits controlled release of nanoparticles leading to changes in magnetic relaxation, enabling detection of enzymatic activity. Further, the presented functionalization scheme extends the scope of potential applications for PPS-b-PEG. Combined with previous findings using this polymer platform that demonstrate controlled drug release in oxidative environments, smart theranostic applications combining drug delivery with imaging of platform localization are within reach. The modular design of these USPIO nanoclusters enables future development of platforms for imaging and drug delivery targeted towards proteolytic activity in tumo... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4527684 Sun, 27 Feb 2011 00:00:00 +0100 4527684 http://www.medworm.com/index.php?rid=4507176&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F6 Conclusion: In this study, the ruthenium(II)-arene complex could significantly induce apoptosis in human lung cancer HCC827 cell line. Treatment of HCC827 cells with the ruthenium(II)-arene complex resulted in dose-dependent cell apoptosis as indicated by high cleaved Caspase-8,9 ratio. Besides ruthenium(II)-arene complex caused a rapid induction of cleaved Caspase-3 activity and stimulated proteolytic cleavage of poly-(ADP-ribose) polymerase (PARP) in vitro and in vivo. Our results suggest that ruthenium(II)-arene complex could be a candidate for further evaluation as a chemotherapeutic agent for human cancers, especially lung cancer. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4507176 Tue, 22 Feb 2011 00:00:00 +0100 4507176 http://www.medworm.com/index.php?rid=4455179&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F4 Conclusions: The efficient and long lasting uptake and retention of SPIONs by MSCs using a protocol employing ferumoxide and protamine may be applicable to patients, since both ferumoxides and protamine are approved for human use. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4455179 Wed, 09 Feb 2011 00:00:00 +0100 4455179 http://www.medworm.com/index.php?rid=4436645&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F3 The use of natural products based on aqueous extract of propolis and lycopene in the skin's protective mechanisms against UVA radiation was evaluated by means of experimental acute inflammation on rat paw edema. The purpose of the study is the evaluation of the inoffensiveness of the propolis lycopene system, by evaluating skin level changes and anti-inflammatory action. The regenerative and protective effect of the aqueous propolis and lycopene extract is due to the fact that it is rich in biologically active substances: tocopherols, flavonoids, amino acids, polyunsaturated fatty acids, the chlorophyll pigment, which are substances with a strong antioxidant action, that contribute to the modification of oxidative stress, especially by reducing the prooxidant processes and enhancing the an... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4436645 Fri, 04 Feb 2011 00:00:00 +0100 4436645 http://www.medworm.com/index.php?rid=4377473&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F9%2F1%2F1 Conclusion: The synthesized 3D nanofibrous structures can promote biomaterial interfacial properties to improve cell-platform surface interaction and develop new functional biomaterials for a variety of biomedical applications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4377473 Thu, 20 Jan 2011 00:00:00 +0100 4377473 http://www.medworm.com/index.php?rid=4277752&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F34 Conclusion: We propose that the difference between the solid and liquid system relates to the obvious difference in the exposure, or residence, time of the Nps with respect to the bacterial cell membrane inasmuch as when small, Np-inhibited colonies were selected and streaked on fresh (i.e., no Nps present) media, growth proceeded normally: e.g., a small, growth-inhibited colony resulted in a plateful of typical S. aureus colonies when streaked on fresh, solid media. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4277752 Tue, 21 Dec 2010 00:00:00 +0100 4277752 http://www.medworm.com/index.php?rid=4272206&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F33 Conclusions: We quantitatively studied the endocytosis of AuNPs with different sizes in various cancer cells. The plasmonic scattering images confirm the size-dependent endocytosis of AuNPs. The 45-nm-AuNP is better for drug delivery due to its higher uptake rate. On the other hand, large AuNPs are immobilized on the cell membrane. They can be used to reconstruct the cell morphology. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4272206 Mon, 20 Dec 2010 00:00:00 +0100 4272206 http://www.medworm.com/index.php?rid=4265443&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F32 Conclusion: Novel LPS+Gd-DOTA were visible by MRI in the inner ear in vivo demonstrating transport from the middle ear to the inner ear and with dynamics that correlated to the status of the perilymph circulation. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4265443 Sat, 18 Dec 2010 00:00:00 +0100 4265443 http://www.medworm.com/index.php?rid=4265444&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F30 Background: Multifunctional magnetic nanoparticles are important class of materials in the field of nanobiotechnology, as it is an emerging area of research for material science and molecular biology researchers. One of the various methods to obtain multifunctional nanomaterials, molecular functionalization by attaching organic functional groups to nanomagnetic materials is an important technique. Recently, functionalized magnetic nanoparticles have been demonstrated to be useful in isolation/detection of dangerous pathogens (bacteria/viruses) for human life. Iron (Fe) based material especially FePt is used in the isolation of ultralow concentrations ( (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4265444 Wed, 15 Dec 2010 00:00:00 +0100 4265444 http://www.medworm.com/index.php?rid=4203101&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F27 A highly specific and novel dual-label time-resolved immunofluorometric assay was developed exploiting the unique emission wavelengths of the intrinsically fluorescent terbium (Tb3+) and europium (Eu3+) tracers for the simultaneous detection of human immunodeficiency virus 1 (HIV-1) and hepatitis B virus (HBV) infections, respectively. HIV-1 infection was detected using a double antigen sandwich format wherein anti-HIV-1 antibodies were captured using an in vivo biotinylated version of a chimeric HIV-1 antigen and revealed using the same antigen labeled with Tb3+ chelate. Hepatitis B surface antigen (HBsAg), which served as the marker of HBV infection, was detected in a double antibody sandwich using two monoclonal antibodies (mAbs), one chemically biotinylated to capture, and the other la... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4203101 Fri, 26 Nov 2010 00:00:00 +0100 4203101 http://www.medworm.com/index.php?rid=4142618&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F26 The development of nanodevices for agriculture and plant research will allow several new applications, ranging from treatments with agrochemicals to delivery of nucleic acids for genetic transformation. But a long way for research is still in front of us until such nanodevices could be widely used. Their behaviour inside the plants is not yet well known and the putative toxic effects for both, the plants directly exposed and/or the animals and humans, if the nanodevices reach the food chain, remain uncertain. In this work we show that magnetic carbon-coated nanoparticles forming a biocompatible magnetic fluid (bioferrofluid) can easily penetrate through the root in four different crop plants (pea, sunflower, tomato and wheat). They reach the vascular cylinder, move using the transpiration ... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4142618 Mon, 08 Nov 2010 00:00:00 +0100 4142618 http://www.medworm.com/index.php?rid=4118609&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F25 Conclusion: IRDye800CW-labeled Fe3O4 nanoparticles provide an effective probe for cell-labeling and in vivo imaging. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4118609 Thu, 28 Oct 2010 23:00:00 +0100 4118609 http://www.medworm.com/index.php?rid=4040419&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F24 Conclusions: Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for Medicago sativa cells, a safe range of 1-5 nM should not be exceeded for biological applications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=4040419 Wed, 06 Oct 2010 23:00:00 +0100 4040419 http://www.medworm.com/index.php?rid=3998814&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F22 Conclusion: We have developed a novel drug delivery system to provide sustained delivery of CF "correctors" and "anti-inflammatories" to the lungs. Moreover, we demonstrate here the therapeutic efficacy of nano-based proteostasis-modulator to rescue Pa-LPS induced CF lung disease. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3998814 Thu, 23 Sep 2010 23:00:00 +0100 3998814 http://www.medworm.com/index.php?rid=3895759&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F21 Conclusions: In comparison to conventional generation of bio-conjugated gold nanoparticles by chemical synthesis and subsequent bio-functionalization, the laser ablation-based in situ conjugation allows the rapid one-step production. Due to high conjugation efficiency and productivity in situ conjugation can be easily used for high throughput generation of gold nanoparticles conjugated with precious biomolecules like aptamers. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3895759 Sun, 22 Aug 2010 23:00:00 +0100 3895759 http://www.medworm.com/index.php?rid=3880732&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F19 Conclusions: Silver nanoparticles are capable of inhibiting a prototype arenavirus at non-toxic concentrations and effectively inhibit arenavirus replication when administered prior to viral infection or early after initial virus exposure. This suggests that the mode of action of viral neutralization by silver nanoparticles occurs during the early phases of viral replication. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3880732 Tue, 17 Aug 2010 23:00:00 +0100 3880732 http://www.medworm.com/index.php?rid=3757754&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F17 Conclusions: We propose that, DCPIP may serve as a potent tool to prevent or disrupt the processes of cell proliferation, tissue angiogenesis and inflammation by directly or indirectly targeting expression of specific cellular factors. We also propose that the activities of DCPIP may be long-lasting and/or enhanced if it is delivered enclosed in specific nanoparticles. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3757754 Wed, 14 Jul 2010 23:00:00 +0100 3757754 http://www.medworm.com/index.php?rid=3753663&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F16 Conclusion: The results obtained disclose the effectual role of AuNPs as an anti-oxidative agent, by inhibiting the formation of ROS, scavenging free radicals; thus increasing the anti-oxidant defense enzymes and creating a sustained control over hyperglycemic conditions which consequently evoke the potential of AuNPs as an economic therapeutic remedy in diabetic treatments and its complications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3753663 Tue, 13 Jul 2010 23:00:00 +0100 3753663 http://www.medworm.com/index.php?rid=3699406&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F14 Conclusions: A FhuA Delta1-160 based reduction triggered release system has been shown to control the compound flux by the presence of only one inner channel sterical hindrance based on 3-(2-pyridyldithio)propionic-acid labeling (amino acid position K556). As a consequence, the release kinetic can be modulated by introducing an opportune number of hindrances. The FhuA Delta1-160 channel embedded in liposomes can be advanced to a universal and compound independent release system which allows a size selective compound release through rationally re-engineered channels. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3699406 Thu, 24 Jun 2010 23:00:00 +0100 3699406 http://www.medworm.com/index.php?rid=3665868&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F13 Conclusions: The findings presented here provide information on the mechanism of QD endocytosis that could be exploited to reduce non-specific targeting, thereby improving specific targeting of QDs in cancer diagnosis and treatment applications. These findings are also important in understanding the cytotoxicity of nanomaterials and in emphasizing the importance of strict environmental control of nanoparticles. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3665868 Mon, 14 Jun 2010 23:00:00 +0100 3665868 http://www.medworm.com/index.php?rid=3648380&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F12 Conclusions: With the data collected from this study, we have demonstrated the great potential of ivy nanoparticles as a sunscreen protective agent, and their increased safety over commonly used metal oxide nanoparticles. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3648380 Tue, 08 Jun 2010 23:00:00 +0100 3648380 http://www.medworm.com/index.php?rid=3569695&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F10 Conclusions: The immobilization of different types of biomolecules in high-density microarrays is a challenging task for biotechnology. The method presented here not only allows for the deposition of DNA at submicrometer resolution but also for proteins and other molecules of biological relevance that can be coupled to biotin. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3569695 Sun, 16 May 2010 23:00:00 +0100 3569695 http://www.medworm.com/index.php?rid=3467669&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F8 A reversible cell labelling method has been developed for non-destructive and non-invasive cell labelling and purification. Our method uses high affinity single strand DNA (ssDNA) aptamers against surface exposed target molecules on cells. The aptamers are subsequently removed from the cell surface using DNase nuclease treatment. We exemplified our method by labelling human acute lymphoblastic leukemia cells with Qdot-ssDNA aptamers, and restoring them to the label-free condition by treatment with Benzonase. Binding of the fluorescent-aptamers to the cells was evaluated by measuring fluorescence intensity and was further confirmed using flow cytometry. Removal of the aptamers can be achieved in ~ 10 min by the DNase nuclease digestion. Incubation of cells with aptamers or with the nuclease... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3467669 Mon, 12 Apr 2010 23:00:00 +0100 3467669 http://www.medworm.com/index.php?rid=3408275&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F7 Conclusion: This study has shown that under the correct conditions, QDs can be routinely used for the imaging of PC12 cells with minimal adverse effects. We have found that PC12 cells are highly susceptible to an increased concentration range of the QDs, while the gelatine coating acts as a barrier towards enhanced toxicity at higher QD concentrations. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3408275 Thu, 25 Mar 2010 00:00:00 +0100 3408275 http://www.medworm.com/index.php?rid=3306740&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F6 Plasmid DNA encoding a luciferase reporter gene was complexed with each of six different hybrid nanoparticles (NPs) synthesized from mixtures of poly (D, L-lactide-co-glycolide acid) (PLGA 50:50) and the cationic lipids DOTAP (1, 2-Dioleoyl-3-Trimethyammonium-Propane) or DC-Chol {3beta-[N-(N', N'-Dimethylaminoethane)-carbamyl] Cholesterol}. Particles were 100-400 nm in diameter and the resulting complexes had DNA adsorbed on the surface (out), encapsulated (in), or DNA adsorbed and encapsulated (both). A luciferase reporter assay was used to quantify DNA expression in 293 cells for the uptake of six different NP/DNA complexes. Optimal DNA delivery occurred for 10e5 cells over a range of 500ng - 10mug of NPs containing 20-30 mug DNA per 1 mg of NPs. Uptake of DNA from NP/DNA complexes was f... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3306740 Wed, 24 Feb 2010 00:00:00 +0100 3306740 http://www.medworm.com/index.php?rid=3302234&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F5 Molecular nanodiagnostics applied to cancer may provide rapid and sensitive detection of cancer related molecular alterations, which would enable early detection even when those alterations occur only in a small percentage of cells. The use of gold nanoparticles derivatized with thiol modified oligonucleotides (Au-nanoprobes) for the detection of specific nucleic acid targets has been gaining momentum as an alternative to more traditional methodologies. Here, we present an Au-nanoparticles based approach for the molecular recognition and quantification of the BCR-ABL fusion transcript (mRNA), which is responsible for chronic myeloid leukemia (CML), and to the best of our knowledge it is the first time quantification of a specific mRNA directly in cancer cells is reported. This inexpensive ... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3302234 Wed, 24 Feb 2010 00:00:00 +0100 3302234 http://www.medworm.com/index.php?rid=3298221&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F4 Conclusions: Palm oil esters-in-water nanoemulsions loaded with vitamin E was successfully formulated and has the potential to be used as nanocosmeceuticals. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3298221 Tue, 23 Feb 2010 00:00:00 +0100 3298221 http://www.medworm.com/index.php?rid=3272852&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F3 Conclusions: An analytical model was developed to determine optimal nanoparticle diameter, concentration and probe density, which allowed efficient and rapid optimisation of assay parameters. Numerical analysis and subsequent associated experimental data suggests that the response of the mass sensitive biosensor system used in conjunction with captured particles was affected by i) the coupled mass of the particle, ii) the proximal contact area between the particle and the sensor surface and iii) the available capture area on the particle and binding dynamics to this capture area. The latter two effects had more impact on the detection limit of the system than any potential enhancement due to added mass from a larger nanoparticle. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3272852 Sun, 14 Feb 2010 00:00:00 +0100 3272852 http://www.medworm.com/index.php?rid=3239404&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F2 We have quantitatively analyzed the confocal spectra of colloidal quantum dots (QDs) in rat endothelial progenitor cells (EPCs) by using Leica TCS SP5 Confocal Microscopy System. By careful measurement of and comparison between the confocal spectra of QDs located inside and outside EPCs, it was shown that the interaction between the QDs and EPCs effectively reduces the radius of the exciton confinement inside the QDs so that the excitonic energy increases and the QD fluorescence peak blueshifts. Furthermore, the EPC environment surrounding the QDs shields the QDs so that the excitation of the QDs inside the cells is relatively weak, whereas the QDs outside the cells can be highly excited. At high excitations, the occupation of the ground excitonic state in the QD outside the cells becomes ... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3239404 Thu, 04 Feb 2010 00:00:00 +0100 3239404 http://www.medworm.com/index.php?rid=3189049&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F8%2F1%2F1 Conclusions: These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3189049 Wed, 20 Jan 2010 00:00:00 +0100 3189049 http://www.medworm.com/index.php?rid=3075381&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F9 In conclusion, we have developed a novel in vivo methodology for the site-specific conjugation of QD's and other artificial structures to target proteins in different intracellular compartments and signaling complexes. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=3075381 Thu, 10 Dec 2009 00:00:00 +0100 3075381 http://www.medworm.com/index.php?rid=2946840&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F8 We report that VEGF and IL-1beta-stimulate endothelial permeability via Src dependent pathway by increasing the Src phosphorylation and Ag-NP block the VEGF-and IL-1beta-induced Src phosphorylation at Y419. These results demonstrate that Ag-NP may inhibit the VEGF-and IL-1beta-induced permeability through inactivation of Src kinase pathway and this pathway may represent a potential therapeutic target to inhibit the ocular diseases such as diabetic retinopathy. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=2946840 Fri, 30 Oct 2009 00:00:00 +0100 2946840 http://www.medworm.com/index.php?rid=2923180&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F6 In conclusion, our data indicate that laser-generated AuNPs present a good alternative to chemically synthesized nanoparticles for use in biomedical applications. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=2923180 Fri, 23 Oct 2009 23:00:00 +0100 2923180 http://www.medworm.com/index.php?rid=2912073&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F5 Conclusions: Suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) results in the formation of swellable nanoparticles of defined composition. PHEMA nanoparticles can potentially be used for the controlled release of the anticancer drug doxorubicin. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=2912073 Mon, 19 Oct 2009 23:00:00 +0100 2912073 http://www.medworm.com/index.php?rid=2416479&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F3 F1-ATPase is an enzyme acting as a rotary nano-motor. During catalysis subunits of this enzyme complex rotate relative to other parts of the enzyme. Here we demonstrate that the combination of two input stimuli causes stop of motor rotation. Application of either individual stimulus did not significantly influence motor motion. These findings may contribute to the development of logic gates using single biological motor molecules. (Source: Journal of Nanobiotechnology) Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=2416479 Fri, 15 May 2009 04:00:00 +0100 2416479 http://www.medworm.com/index.php?rid=2330888&cid=s_34079_174_f&fid=34079&url=http%3A%2F%2Fwww.jnanobiotechnology.com%2Fcontent%2F7%2F1%2F1 Antisense oligonucleotides (AOs) have been shown to induce dystrophin expression in muscles cells of patients with Duchenne Muscular Dystrophy (DMD) and in the mdx mouse, the murine model of DMD. However, ineffective delivery of AOs limits their therapeutic potential. Copolymers of cationic poly(ethylene imine) (PEI) and non-ionic poly(ethylene glycol) (PEG) form stable nanoparticles when complexed with AOs, but the positive surface charge on the resultant PEG-PEI-AO nanoparticles limits their biodistribution. We adapted a modified double emulsion procedure for encapsulating PEG-PEI-AO polyplexes into degradable polylactide-co-glycolic acid (PLGA) nanospheres. Formulation parameters were varied including PLGA molecular weight, ester end-capping, and sonication energy/volume. Our results sh... Journal of Nanobiotechnology journals http://www.medworm.com/rss/comments.php?id=2330888 Tue, 07 Apr 2009 04:00:00 +0100 2330888