MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Letters in Applied Microbiology' source. Thu, 09 Feb 2012 21:35:07 +0100 http://www.medworm.com/index.php?rid=5674656&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03218.x Conclusions:  Supplementation of TSB with Tween 80® allows growth of LAB to levels similar to that observed with MRS but does not inhibit the growth of E. coli O157:H7 and Salmonella. We present the formulation of a medium useful in studies useful for evaluating competitive inhibition of foodborne pathogens by LAB in vitro.Significance and Impact of the Study:  This study reports the utility of TSB‐T80 for the completion of in vitro competitive inhibition assays incorporating a Lactic Acid Bacteria food safety culture.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674656 Tue, 07 Feb 2012 05:00:00 +0100 5674656 http://www.medworm.com/index.php?rid=5674655&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03219.x Conclusion:  This study suggests that SN have antifungal therapeutic potential, but further studies are still required namely regarding formulation and delivery means.Significance and Impact of Study:  SN may contribute to the development of new strategies for the improvement of oral health and quality of life particularly of the complete denture wearers.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5674655 Wed, 01 Feb 2012 05:00:00 +0100 5674655 http://www.medworm.com/index.php?rid=5657236&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03217.x Conclusions:  Optimized culture conditions for maximizing yeast biomass production, determined in flask cultures was applicable at a larger scale. The highest yield values were attained in media containing relative low CMz concentrations supplemented with DAP and YE. YE would not be necessary if higher productivity is the aim.Significance and Impact of the Study:  Cells of L. thermotolerans could be produced aerobically could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources. RSM allowed the fine tuning of cultural conditions.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5657236 Wed, 01 Feb 2012 05:00:00 +0100 5657236 http://www.medworm.com/index.php?rid=5644301&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03216.x Conclusions:  The majority of interactions identified synergistic and additive effects, with strong synergism noted between B. papyrifera with C. myrrha.Significance and Impact of the Study:  Frankincense and myrrh essential oils have been used in combination since 1500 BC, however, no antimicrobial investigations have been undertaken to confirm their effect in combination. This study validates the enhanced efficacy when used in combination against a selection of pathogens.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5644301 Tue, 31 Jan 2012 20:36:17 +0100 5644301 http://www.medworm.com/index.php?rid=5635980&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03214.x Conclusion:  We were successful in isolating several Francisella strains from natural warm springs; however, none appeared to genetically match the original 2001 clinical isolate.Significance and Impact of Study:  This work highlights the presence of viable, potentially pathogenic, Franscisella species living in the unique environmental niche of natural warm springs.© No claim to US Government works. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635980 Sat, 28 Jan 2012 02:24:27 +0100 5635980 http://www.medworm.com/index.php?rid=5635983&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03212.x Conclusions:  These results reflected that there exists a rather high contamination level in home‐made tiger‐nut beverages indicating the need to apply correct and strict HACCP system(s) during manufacturing and storage of these food products.Significance and Impact of Study:  This study demonstrates the great need to carry out microbiological tests frequently in these products and even more the need to apply correct HACCP system (s). Tiger‐nut beverages are especially well‐known products in Spain, hence it is extremely important to ensure an adequate microbiological quality to guarantee consumers health.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635983 Fri, 27 Jan 2012 05:00:00 +0100 5635983 http://www.medworm.com/index.php?rid=5635982&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03213.x Conclusion:  BAM‐CSRF is a useful new selective medium for isolation of B. hyodysenteriae and other intestinal spirochaetes from pig faeces.Significance and Impact of the Study:  The new selective medium for isolating B. hyodysenteriae and other Brachyspira spp. from pig faeces will improve their recovery and subsequent disease diagnosis.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635982 Fri, 27 Jan 2012 05:00:00 +0100 5635982 http://www.medworm.com/index.php?rid=5635981&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03215.x Conclusions:  The protein profile of S. commune confronted by T. viride indicates the up‐regulation of mechanisms specifically targeted at the mycoparasitic machinery of T. viride particularly cell wall lysis and antibiosis.Significance and Impact of the Study:  The proteomic responses observed in S. commune may occur in natural environments, providing an insight to the mechanism involved in conferring resistance to mycoparasitic attack. This study therefore, warrants further investigation for the targeted design of more robust biocontrol agents.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5635981 Fri, 27 Jan 2012 05:00:00 +0100 5635981 http://www.medworm.com/index.php?rid=5625260&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03211.x In this study we investigated the ability of HHP to inactivate murine norovirus (MNV‐1), a recognised surrogate for NoV, in experimentally contaminated manila clams. Pools of contaminated live clams were subjected to hydrostatic pressure ranging from 300 to 500 MPa for different time intervals of between one and 10 min. The trial was repeated three times, at monthly intervals. Virus vitality post‐treatment was assessed and the data obtained indicates that the use of high hydrostatic pressures of at least 500 MPa for 1 min was effective in inactivating MNV‐1.© No claim to Italian Government works (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625260 Wed, 25 Jan 2012 03:52:15 +0100 5625260 http://www.medworm.com/index.php?rid=5625262&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03209.x Conclusions:  Several putative virulence factors and known factors responsible for sporulation were differentially regulated, including CodY, pXO1‐130 and BA1952, revealing insights into temperature cues in the B. anthracis secretome.Significance and Impact of the Study:  This study identified temperature‐regulated proteins. Further studies aimed at understanding the physical and functional roles of these proteins in infection and control by elevated temperatures will contribute to detection, diagnostics and prophylaxis.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625262 Mon, 23 Jan 2012 05:00:00 +0100 5625262 http://www.medworm.com/index.php?rid=5625261&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03210.x Conclusion:  Identification of DGGE bands is possible by comparison of HRM melting profiles with known reference strains.Significance and Impact of study:  HRM profiling is suggested as an additional approach for identification of DGGE bands.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625261 Mon, 23 Jan 2012 05:00:00 +0100 5625261 http://www.medworm.com/index.php?rid=5617484&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03208.x Conclusions:  This study describes the full length sequence of a plasmid carrying the qnrS2 gene from A. hydrophila. The plasmid pAHH04 carried plasmid replication and mobilization genes from IncQ type plasmids.Significance and Impact of the Study:  The isolated qnrS2 gene encoded by a plasmid from an A. hydrophila strain is of significant importance because it emphasizes the problem of antibiotic resistance as well as the ability of the determinants to spread among the different bacterial species that impact human health.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5617484 Sun, 22 Jan 2012 02:07:44 +0100 5617484 http://www.medworm.com/index.php?rid=5604510&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03207.x Conclusions:  Despite limited contact with humans, pigs raised outdoors are colonized by an MRSA population that genetically overlaps with that of intensively farmed pigs, though antimicrobial resistance is lower.Significance and impact of the study:  To our knowledge, this is the first detection of MRSA in food animals raised in free‐range conditions.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604510 Thu, 19 Jan 2012 02:49:00 +0100 5604510 http://www.medworm.com/index.php?rid=5604515&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03198.x Conclusions:  Reverse transcription loop‐mediated isothermal amplification is a novel, alternative microbiological approach for rapid, sensitive and specific detection of EV71 in HFMD.Significance and Impact of the Study:  Reverse transcription loop‐mediated isothermal amplification assay is suitable for the diagnosis of EV71 infection as a routine diagnostic tool for HFMD because of fewer requirements of experimental conditions such as private clinics, field applications as well as an epidemiological survey in epidemic areas. RT‐LAMP can also be used as an alternative method for EV71 detection. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604515 Wed, 18 Jan 2012 05:00:00 +0100 5604515 http://www.medworm.com/index.php?rid=5604514&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03201.x Conclusions:  This technique was successfully applied to detect viable cells in inoculated faeces.Significance and Impact of the Study:  Results showed that this DVC‐FISH procedure is a quick and culture‐independent useful method to specifically detect viable Lact. delbrueckii subsp. bulgaricus and Strep. thermophilus in different samples, being applied for the first time to lactic acid bacteria. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604514 Wed, 18 Jan 2012 05:00:00 +0100 5604514 http://www.medworm.com/index.php?rid=5604513&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03202.x Conclusions:  Immunization with EPEC ghosts can elicit protective immune responses in BALB/c mice.Significance and Impact of the Study:  EPEC ghosts may represent a promising new approach for vaccination against EPEC infection. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604513 Wed, 18 Jan 2012 05:00:00 +0100 5604513 http://www.medworm.com/index.php?rid=5604512&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03205.x Conclusions:  In general our findings demonstrate the potential use of a modified phage combination for the prevention and successful treatment of Staph., aureus biofilms, which are implicated in several antibiotic resistant infections.Significance and Impact of the Study:  This study highlights the first use of phage K for the successful removal and prevention of biofilms of Staph., aureus.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604512 Tue, 17 Jan 2012 05:00:00 +0100 5604512 http://www.medworm.com/index.php?rid=5604511&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03206.x Conclusions: A highly specific and rapid assay was developed for the detection of M. morganii in tuna fish samples.Significance and Impact of the Study: This method represents the first study about the quantification of pathogenic M. morganii in fish products. This approach can be utilized to prevent the presence of this undesirable species in the food chain.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5604511 Tue, 17 Jan 2012 05:00:00 +0100 5604511 http://www.medworm.com/index.php?rid=5594036&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03199.x Conclusions: Lactobacillus plantarum CECT 7315/CECT 7316 because of their potential probiotic properties could be excellent candidates for being tested in clinical trials aimed to demonstrate beneficial effects on human health.Significance and Impact of the Study:  Probiotics are live micro‐organisms that confer a health benefit for the host. However, not all the lactic acid bacteria possess the ability to confer health benefits for the host. In this study, two Lact. plantarum strains (CECT 7315 and CECT 7316) were isolated and characterized to demonstrate their excellent qualities as potential probiotic strains. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5594036 Fri, 13 Jan 2012 05:00:00 +0100 5594036 http://www.medworm.com/index.php?rid=5577116&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03204.x Conclusions:  IS1004 profiling can be a reliable method for analysis of clonal dissemination of V. cholerae. The results indicated that specific point mutation at a particular position within the wbeT of V. cholerae O1 strains in Iran may occur which, in turn, may result in serotype switching.Significance and Impact of the Study:  Understanding the molecular basis for serotype conversion of V. cholerae and their genetic relatedness could give insights for the incoming cholera epidemic prediction and control.© 2012 The Authors Letters in Applied Microbiology© 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5577116 Sun, 01 Jan 2012 05:00:00 +0100 5577116 http://www.medworm.com/index.php?rid=5569706&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2012.03203.x Conclusions:  Systematic optimization of RAPD generated reliable DNA fingerprints for nosocomial isolates of K. pneumoniae.Significance and impact of the study:  This is the first report on RAPD optimization based on factorial design of experiments for discrimination of K. pneumoniae.© 2012 The Authors Letters in Applied Microbiology© 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569706 Sun, 01 Jan 2012 05:00:00 +0100 5569706 http://www.medworm.com/index.php?rid=5542488&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03202.x Conclusions:  Immunization with EPEC ghosts can elicit protective immune responses in BALB/c mice.Significance and Impact of the Study:  EPEC ghosts may represent a promising new approach for vaccination against EPEC infection.© 2011 The Authors Letters in Applied Microbiology © 2011 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5542488 Mon, 26 Dec 2011 14:23:12 +0100 5542488 http://www.medworm.com/index.php?rid=5534257&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03200.x Conclusions:  The presence of maltose and ethanolamine indicate a state of acute nutrient limitation; therefore, we hypothesize that the cell’s metabolism turned to its own cell wall, or perhaps neighboring cells, for sources of carbon and nitrogen. The presence of the BTC also supports the acute nutrient limitation idea due to the parallels with polyhydroxyalkanoate (PHA) production in other Gram‐negative bacteria, including other Vibrio species.Significance and Impact of the Study:  Recent metabolomics research on the temperature‐dependent coral pathogen Vibrio coralliilyticus has led to the discovery of several compounds produced by the organism as a response to high density, low nutrient conditions. The three metabolites, along with 1H NMR metabolic fingerprints of the nutrie... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534257 Fri, 23 Dec 2011 15:06:02 +0100 5534257 http://www.medworm.com/index.php?rid=5625264&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03199.x Conclusions: Lactobacillus plantarum CECT 7315/CECT 7316 because of their potential probiotic properties could be excellent candidates for being tested in clinical trials aimed to demonstrate beneficial effects on human health.Significance and Impact of the Study:  Probiotics are live micro‐organisms that confer a health benefit for the host. However, not all the lactic acid bacteria possess the ability to confer health benefits for the host. In this study, two Lact. plantarum strains (CECT 7315 and CECT 7316) were isolated and characterized to demonstrate their excellent qualities as potential probiotic strains. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625264 Wed, 21 Dec 2011 05:00:00 +0100 5625264 http://www.medworm.com/index.php?rid=5625263&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03201.x Conclusions:  This technique was successfully applied to detect viable cells in inoculated faeces.Significance and Impact of the Study:  Results showed that this DVC‐FISH procedure is a quick and culture‐independent useful method to specifically detect viable Lact. delbrueckii subsp. bulgaricus and Strep. thermophilus in different samples, being applied for the first time to lactic acid bacteria. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5625263 Wed, 21 Dec 2011 05:00:00 +0100 5625263 http://www.medworm.com/index.php?rid=5534259&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03199.x Conclusions: L. plantarum CECT 7315 /CECT 7316 due to their potential probiotic properties could be excellent candidates for being tested in clinical trials aimed to demonstrate beneficial effects on human health.Significance and Impact of the Study:  Probiotics are live microorganisms which confer a health benefit for the host. However, not all all lactic acid bacteria possess the ability to confer health benefits for the host. In this study, two Lactobacillus plantarum strains (CECT 7315 and CECT 7316) were isolated and characterized to demonstrate their excellent qualities as potential probiotic strains.© 2011 The Authors Letters in Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534259 Wed, 21 Dec 2011 05:00:00 +0100 5534259 http://www.medworm.com/index.php?rid=5534258&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03201.x Conclusions:  This technique was successfully applied to detect viable cells in inoculated feces.Significance and Impact of the Study:  Results showed that this DVC‐FISH procedure is a quick and culture‐independent useful method to specifically detect viable L. delbrueckii subsp. bulgaricus and S. thermophilus in different samples, being applied for the first time to Lactic Acid Bacteria (LAB).© 2011 The Authors Letters in Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534258 Wed, 21 Dec 2011 05:00:00 +0100 5534258 http://www.medworm.com/index.php?rid=5512995&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03197.x Conclusions: S. aureus was detected and differentiated from S. epidermidis in one hour with a novel FISH method that used a single hot‐plate and in the absence of dimethyl formamide.Significance and Impact of Study:  A rapid hot‐plate FISH assay with urea‐NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.© 2011 The Authors Letters in Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5512995 Sat, 17 Dec 2011 14:39:30 +0100 5512995 http://www.medworm.com/index.php?rid=5569707&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03197.x Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1 h with a novel FISH method that used a single hot‐plate and in the absence of dimethyl formamide.Significance and Impact of Study:  A rapid hot‐plate FISH assay with urea‐NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569707 Fri, 16 Dec 2011 05:00:00 +0100 5569707 http://www.medworm.com/index.php?rid=5512997&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03195.x Conclusions:  A more oxidative stable beer is not obtained by a more oxidative stress tolerant lager brewing yeast strain, exhibiting a higher secretion of thioredoxin, but rather by a less oxidative stress tolerant strain, exhibiting a higher iron uptake.Significance and Impact of the Study:  In order to obtain lager beers with enhanced oxidative stability, yeast strains should be screened for their low oxidative stress tolerance and/or high ability to take up iron rather than for their high oxidative stress tolerance and/or high ability to secrete thioredoxin.© 2011 The Authors Letters in Applied Microbiology© 2011 The Society for Applied Microbiology (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5512997 Fri, 16 Dec 2011 05:00:00 +0100 5512997 http://www.medworm.com/index.php?rid=5512996&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03196.x Conclusions:  The strain was capable of growing in atmospheres containing no or limited amounts of oxygen and increased amounts of carbon dioxide. Even when 100% CO2 was used, growth could not be inhibited completely.Significance and Impact of Study:  In contrast to the general characteristics of the genus Pseudomonas (strictly aerobic, highly sensitive to CO2), it should not be expected in the food industry that removing oxygen from the food package and increasing the carbon dioxide content, combined with cold storage, will easily avoid spoilage by Pseudomonas species. Guarantee of hygienic standards and combination of strategies with other microbial growth inhibiting measures should be implemented.© 2011 Kempen University College Letters in Applied Microbiology© 2011 The Society fo... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5512996 Fri, 16 Dec 2011 05:00:00 +0100 5512996 http://www.medworm.com/index.php?rid=5501741&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03194.x Conclusions:  No changes in net K+ flux from heat‐stressed E. coli cells were observed directly as a result of thermal treatments. However, regardless of the magnitude of heat treatment above 55°C, loss of viability indicated by enrichment culture, correlated with disrupted K+ fluxes when previously heated cells were further challenged by imposing hyperosmotic stress during flux measurement. This two‐stage process enabled evaluation of the lethality of heat treated bacterial cells within 2 h and may be an alternative and more rapid method to confirm the lethality of heat treatment.Significance and Impact of the Study:  The ability to confirm the lethality of thermal treatments and to specify minimal time/temperature combinations by a non‐culture dependent test offers an alternat... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501741 Wed, 14 Dec 2011 16:06:32 +0100 5501741 http://www.medworm.com/index.php?rid=5569708&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03193.x Conclusions:  Acetaldehyde‐ and pyruvic acid‐bound SO2 were inhibitory to wine LAB growth at concentrations as low as 5 mg l−1. Despite this inhibition, all wine LAB degraded SO2‐bound acetaldehyde and pyruvic acid suggesting that bound SO2 may have a bacteriostatic rather than bacteriocidal action.Significance and Impact of the Study:  Sulphur dioxide bound to acetaldehyde or pyruvic acid is inhibitory to growth of wine LAB and must be considered when conducting the malolactic fermentation or controlling the growth of spoilage bacteria such as Pediococcus and Lactobacillus. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569708 Mon, 12 Dec 2011 05:00:00 +0100 5569708 http://www.medworm.com/index.php?rid=5501744&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03192.x Conclusion:  Biofilm‐associated E. coli can affect microbial quality of irrigation water, and therefore should not be neglected when estimating bacterial mass balances for irrigation systems.Significance and Impact of the Study:  This work is the first peer‐reviewed report on the impact of biofilms on microbial quality of irrigation waters. Flushing of the irrigation system may be a useful management practice to decrease the risk of microbial contamination of produce. Because microbial water quality can be substantially modified while water is transported in an irrigation system, it becomes imperative to monitor water quality at fields, rather than just at the intake. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501744 Mon, 12 Dec 2011 05:00:00 +0100 5501744 http://www.medworm.com/index.php?rid=5501743&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03193.x Conclusions:  Acetaldehyde and pyruvic acid bound SO2 was inhibitory to wine LAB growth at concentrations as low as 5 mg l−1. Despite this inhibition, all wine LAB degraded SO2 bound acetaldehyde and pyruvic acid suggesting that bound SO2 may have a bacteriostatic rather than bacteriocidal action.Significance and Impact of Study:  SO2 bound to acetaldehyde or pyruvic acid is inhibitory to growth of wine LAB and must be considered when conducting the malolactic fermentation or controlling the growth of spoilage bacteria such as Pediococcus and Lactobacillus. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501743 Mon, 12 Dec 2011 05:00:00 +0100 5501743 http://www.medworm.com/index.php?rid=5493026&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03191.x Conclusion:  Distinct changes occurred in the bacterial community in guinea grass silage because of wilting and molasses addition. These changes could explain how lactic acid fermentation was enhanced but could not help determine which bacteria were associated with enhanced acetic acid fermentation.Significance and Impact of the Study:  The study reveals the effects of wilting and molasses during ensiling of low DM tropical grasses and the associated bacteria. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5493026 Sun, 11 Dec 2011 16:00:33 +0100 5493026 http://www.medworm.com/index.php?rid=5465775&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03190.x SummaryEssential oil (EO) vapours have been known for their antimicrobial properties since the 4th century B.C., however, it was not until the early 1960s that research into the potential of these volatile oils was explored. More recently the use of EOs such as tea tree, bergamot, lavender and eucalyptus in vapour form have been shown to have antimicrobial effects against both bacteria and fungi, with range of methods being developed for dispersal and efficacy testing. To date many applications for EO vapours as antimicrobials have been identified including in the food and clinical arenas. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465775 Thu, 01 Dec 2011 05:00:00 +0100 5465775 http://www.medworm.com/index.php?rid=5465774&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03189.x Conclusions:  In addition to the proven in‐vivo activity of tempe, this confirms the potential anti‐diarrhoeal effect of both the soy products tempe and tofu.Significance and Impact of the Study:  Since tofu has a much greater circle of consumers this finding is relevant for the health of a large part of the world’s population. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465774 Thu, 01 Dec 2011 05:00:00 +0100 5465774 http://www.medworm.com/index.php?rid=5454453&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03186.x Conclusions:  Direct plasma exposure significantly inactivated seeded bacterial cells by approximately 6 log10 on agar plates and 2‐3 log10 on porcine skin. On agar plates, an indirect “bystander” inactivation outside the plasma delivery area was also observed. The reduced inactivation observed on the skin surface was most likely due to cell protection by the variable surface architecture.Significance and Impact of Study:  Atmospheric plasma has potential for clinical application as a disinfectant of patient skin and medically relevant surfaces. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5454453 Tue, 29 Nov 2011 15:00:48 +0100 5454453 http://www.medworm.com/index.php?rid=5534263&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03186.x Conclusions:  Direct plasma exposure significantly inactivated seeded bacterial cells by approx. 6 log10 on agar plates and 2–3 log10 on porcine skin. On agar plates, an indirect ‘bystander’ inactivation outside the plasma delivery area was also observed. The reduced inactivation observed on the skin surface was most likely due to cell protection by the variable surface architecture.Significance and Impact of Study:  Atmospheric plasma has potential for clinical application as a disinfectant of patient skin and medically relevant surfaces. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5534263 Tue, 29 Nov 2011 05:00:00 +0100 5534263 http://www.medworm.com/index.php?rid=5465777&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03188.x Conclusions:  Vibrios with a broad phenotypic variability were found in the external lesions of diseased Atherina boyeri. In total 3 species of Vibrio were identified: V. harveyi showed the wider phenotypical and ribotypical heterogeneity while Listonella anguillarum shared similar biochemical characteristics with typical strains.Significance and Impact of the study:  Previously unreported co‐existence of potential pathogenic species colonizing diseased Atherina boyeri has ecological as well as epidemiological significance. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465777 Tue, 29 Nov 2011 05:00:00 +0100 5465777 http://www.medworm.com/index.php?rid=5465776&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03187.x Conclusions:  The RT‐PCR was more sensitive than culture for H. parasuis detection in the organs compared.Significance and impact of the study:  The RT‐PCR evidenced that NPAPT vaccines were those yielding the best protection results in terms of H. parasuis clearance. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5465776 Tue, 29 Nov 2011 05:00:00 +0100 5465776 http://www.medworm.com/index.php?rid=5447962&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03185.x Conclusions:  At least two methods should be used concurrently for primary isolation of M. bovis from bovine tissues, particularly for paucibacillary samples.Significance and Impact of Study:  Detection of low numbers of M. bovis in tissue is an important goal in optimizing detection of bovine tuberculosis, and should assist in identification of infected cattle, in particular, those with few M. bovis bacilli. This was apparently the first study comparing three decontamination methods for detection of Mycobacterium bovis in paucibacillary samples from naturally infected cattle. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447962 Sat, 26 Nov 2011 15:39:43 +0100 5447962 http://www.medworm.com/index.php?rid=5447964&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03184.x Conclusions:  The increased acyl‐CoA levels across the strains profiled in this study reflect the genetic modifications implemented for improved polyketide production and also indicate flexibility of the native PrpE.Significance and Impact of Study:  The results provide direct evidence of enhanced acyl‐CoA levels correlating to those strains engineered for polyketide biosynthesis. This information and the inherent flexibility of the native PrpE enzyme support future efforts to characterize, engineer, and extend acyl‐CoA precursor supply for additional heterologous biosynthetic attempts. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447964 Sat, 26 Nov 2011 05:00:00 +0100 5447964 http://www.medworm.com/index.php?rid=5447963&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03183.x Conclusion: A significant number (n=10) of Harveyi clade Vibrio strains have been inaccurately identified, including evidence that V. communis and V. owensii strains, two recently discovered species assigned to the Harveyi clade, comprise a single species.Significance and Impact of this study:  Since Harveyi clade vibrios have an enormous impact on human and aquatic animal health, it is of paramount importance to identify members of the Harveyi clade correctly. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5447963 Sat, 26 Nov 2011 05:00:00 +0100 5447963 http://www.medworm.com/index.php?rid=5437725&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03182.x Conclusion:  The in vivo gene transfer of HLRG from E. faecalis strains, isolated from food of animal origin, to human microbiota has been demonstrated in a mouse model.Significance and Impact of the Study:  The complexity found on the therapeutic responses of invasive infectious diseases caused by E. faecalis, facilitates the assessment of food of animal origin as a resistant‐pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials resistance gene transfer between E. faecalis strains from food and human GI tract. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5437725 Wed, 23 Nov 2011 14:26:40 +0100 5437725 http://www.medworm.com/index.php?rid=5428638&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03181.x Conclusion:  Our results confirm the seed transmission of this corn salad disease. Pathogen load and distribution vary between positively tested seed lots.Significance and Impact of the study:  With this method not only routine testing of seed material in order to eliminate contaminated seed lots from production is possible but also the control of sanitation procedures to reduce contamination. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428638 Sun, 20 Nov 2011 14:44:25 +0100 5428638 http://www.medworm.com/index.php?rid=5501748&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03182.x Conclusions:  The in vivo gene transfer of HLRG from Ent. faecalis strains, isolated from the food of animal origin, to human microbiota has been demonstrated in a mouse model.Significance and Impact of the Study:  The complexity found on the therapeutic responses of invasive infectious diseases caused by Ent. faecalis facilitates the assessment of food of animal origin as a resistant pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials’ resistance gene transfer between Ent. faecalis strains from food and human GI tract. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501748 Sun, 20 Nov 2011 05:00:00 +0100 5501748 http://www.medworm.com/index.php?rid=5418541&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03178.x Conclusions:  The cyanobacterial bloom material can be used as a glucose substitute in white rot fungi medium. The white rot fungi, Trichaptum abietinum 1302BG and Lopharia spadicea, can also directly decrease the biomass of cyanobacterial bloom material taken from Taihu Lake.Significance and Impact of Study:  Cyanobacterial bloom thrive in eutrophic fresh waters all over the world. Microorganisms, particularly fungi, have attracted attention as possible agents for the degradation of phytoplankton species. Dealing with cyanobacterial bloom material as a medium for fungi instead of directly discharging them as organic fertilizers is a new, safe, and environmentally friendly approach. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418541 Fri, 18 Nov 2011 05:04:40 +0100 5418541 http://www.medworm.com/index.php?rid=5501750&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03180.x Conclusions: At. ferrooxidans possesses a functional c‐di‐GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes.Significance and Impact of the Study:  This is the first global study about the c‐di‐GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining micro‐organisms during the bioleaching process. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501750 Fri, 18 Nov 2011 05:00:00 +0100 5501750 http://www.medworm.com/index.php?rid=5501749&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03179.x Conclusions:  Copper field sprays mixed with water that may contain E. coli can help eliminate E. coli from plant surfaces.Significance and Impact of the Study:  HACCP mandates are becoming more restrictive because of the increased illness resulting from food pathogens on fresh produce. Use of potable water in fields, a proposed mandate, is not feasible for large grove owners. These data show that copper sprays aimed at reducing citrus canker also affect E. coli and may help to ease water quality mandates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5501749 Fri, 18 Nov 2011 05:00:00 +0100 5501749 http://www.medworm.com/index.php?rid=5428640&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03179.x Conclusions:  Copper field sprays mixed with water that may contain E. coli can help eliminate E. coli from plant surfaces.Significance and Impact of Study:  HACCP mandates are becoming more restrictive due to the increased illness resulting from food pathogens on fresh produce. Use of potable water in fields, a proposed mandate, is not feasible for large grove owners. These data show that copper sprays aimed at reducing citrus canker also affect E. coli and may help to ease water quality mandates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428640 Fri, 18 Nov 2011 05:00:00 +0100 5428640 http://www.medworm.com/index.php?rid=5428639&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03180.x Conclusions: At. ferrooxidans possesses a functional c‐di‐GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes.Significance and Impact of the Study:  This is the first global study about the c‐di‐GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining microorganisms during the bioleaching process. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5428639 Fri, 18 Nov 2011 05:00:00 +0100 5428639 http://www.medworm.com/index.php?rid=5569710&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03176.x Conclusions:  This report demonstrated that the 20‐kDa chaperone‐like protein enhances yield, volume and solubility of the coleopteran Cry3A crystalline inclusions per unit crystal/spore mixture.Significance and Impact of the Study:  This is the first report showing that an accessory protein (20‐kDa) could enhance synthesis and crystallization of Cry3A, a finding that could be beneficial for commercial production of this coleopteran‐specific insecticidal protein for microbial insecticides and possibly even for transgenic crops. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569710 Tue, 15 Nov 2011 05:00:00 +0100 5569710 http://www.medworm.com/index.php?rid=5569709&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03177.x Conclusions:  Within a short incubation period, significantly high α‐amylase activity was obtained and it is higher than those reported to date at bioreactor scale operating with a fungal strain.Significance and Impact of the Study:  The reactor is novel and can overcome some of the major problems associated with SSF process. A. oryzae IFO 30103 is reported as the best fungal source for α‐amylase production. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5569709 Tue, 15 Nov 2011 05:00:00 +0100 5569709 http://www.medworm.com/index.php?rid=5418544&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03175.x Conclusions:  The RuBGX1 shows β‐glucosidase activity in hydrolysis of cello‐oligosaccharides, meanwhile it has β‐xylosidase activity and functions synergistically with endoxylanase to promote the degradation of hemicellulosic xylans.Significance and Impact of the study:  This was the first report that the β‐xylosidase activity of family 3 β‐glucosidase/xylosidase functioned in degradation of hemicellulosic xylans. The bifunctional β‐glucosidase/xylosidase property of RuBGX1 can be used in simultaneous saccharification of cellulose and xylan into fermentable glucose and xylose. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418544 Tue, 15 Nov 2011 05:00:00 +0100 5418544 http://www.medworm.com/index.php?rid=5418543&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03176.x Conclusions:  This report demonstrated that the 20‐kDa chaperone‐like protein enhances yield, volume and solubility of the coleopteran Cry3A crystalline inclusions per unit crystal/spore mixture.Significance and Impact of the Study:  This is the first report showing that an accessory protein (20‐kDa) could enhance synthesis and crystallization Cry3A, a finding that could be beneficial for commercial production of this coleopteran‐specific insecticidal protein for microbial insecticides and possibly even for transgenic crops. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418543 Tue, 15 Nov 2011 05:00:00 +0100 5418543 http://www.medworm.com/index.php?rid=5418542&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03177.x Conclusions:  Within a short incubation period significantly high α‐amylase activity was obtained and it is higher than those reported to date at bioreactor scale operating with a fungal strain.Significance and Impact of Study:  The reactor is novel and can overcome some of the major problems associated with SSF process. A. oryzae IFO 30103 is reported as the best fungal source for α‐amylase production. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5418542 Tue, 15 Nov 2011 05:00:00 +0100 5418542 http://www.medworm.com/index.php?rid=5397535&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03174.x Conclusions:  The groEL gene is a potential marker for the species‐specific detection of V. parahaemolyticus, and could be used to detect this bacterium in contaminated food by PCR.Significance and Impact of the Study:  PCR using primers designed from groEL gene provide an efficient method for the accurate identification of V. parahaemolyticus from contaminated samples. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5397535 Tue, 01 Nov 2011 04:00:00 +0100 5397535 http://www.medworm.com/index.php?rid=5376547&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03171.x Conclusions:  A procedure is provided for selecting glucose‐utilizing R. eutropha H16 in an unprecedented short time period and without any mutagenic treatment. An altered N‐acetylglucosamine phosphotransferase system appears to be a common motif in all glucose‐utilizing mutants examined so far.Significance and Impact of the Study:  The correlation of the applied glucose concentration and the appearance of glucose‐utilizing mutants poses questions about the randomness or the specificity of adaptive mutations in general. Furthermore, glucose‐adapted strains of R. eutropha H16 could be useful for the production of bioplastics. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376547 Tue, 01 Nov 2011 04:00:00 +0100 5376547 http://www.medworm.com/index.php?rid=5376546&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03172.x Conclusions:  Our results show that SmrepPCR‐inferred relationship of isolates is in a good agreement with sequence‐based methods. The combined information from all methods used suggests that rapid evolution of S. maltophilia genomes might be predominantly due to high rate of rearrangements caused by mobile genetic elements.Significance and Impact of the Study:  The presented method is an inexpensive and easy to perform alternative to genotype S. maltophilia isolates and to study their population genetics. SmrepPCR demonstrates the usefulness of species‐specific repetitive elements in genomic analyses. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376546 Tue, 01 Nov 2011 04:00:00 +0100 5376546 http://www.medworm.com/index.php?rid=5376545&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03173.x Conclusions:  The occurrence of unrelated multiresistant E. coli in faecal flora of captive specimens of Iberian lynx, including the presence of ESBLs, resistant genes in integrons, and virulence determinants was showed in this study.Significance and Impact of the study:  The results obtained in this study highlight the environmental problem as future reintroductions of Iberian lynx could lead to a spread of resistant bacteria. Additionally, ESBL‐producing bacteria can represent a health problem for this endangered species. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376545 Tue, 01 Nov 2011 04:00:00 +0100 5376545 http://www.medworm.com/index.php?rid=5376548&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03170.x Conclusion:  Subtilosin likely acts upon L. monocytogenes Scott A by perturbing the lipid bilayer of the cellular membrane and causing intracellular damage, leading to eventual cell death. Subtilosin’s mode of action against L. monocytogenes Scott A differs from the one previously described for another human pathogen, Gardnerella vaginalis.Significance and Impact:  This is the first report on the specific mode of action of subtilosin against L. monocytogenes, and the first report of a bacteriocin with a species‐specific mode of action. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376548 Mon, 31 Oct 2011 04:00:00 +0100 5376548 http://www.medworm.com/index.php?rid=5356027&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03169.x Conclusions:  The application of chemometric tools allows the optimization of a bioassay that detects betalactams residues in milk. The more robust conditions have be achieved in Log[S] = 6.30 and [It] = 4.20 h.Significance and Impact of the Study:  The logistic regression model and the desirability function are adequate chemometric techniques to improve the properties of the methods, since it is possible to increase sensitivity and decrease cross‐specificity simultaneously. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5356027 Fri, 28 Oct 2011 07:22:19 +0100 5356027 http://www.medworm.com/index.php?rid=5344028&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03166.x Conclusions:  Oral administration of Lact. fermentum CRL1446 to mice increases total intestinal FE activity, decreases the basal percentage of plasmatic lipoperoxides and increases GR activity.Significance and Impact of Study: Lact. fermentum CRL1446 could be orally administered as a dietary supplement or functional food for increasing the intestinal FE activity to enhance the bioavailability of ferulic acid, thus improving oxidative status. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5344028 Mon, 24 Oct 2011 12:11:43 +0100 5344028 http://www.medworm.com/index.php?rid=5437726&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03169.x Conclusions:  The application of chemometric tools allows the optimization of a bioassay that detects betalactam residues in milk. The more robust conditions have been achieved in Log[S] = 6·30 and [It] = 4·20 h.Significance and Impact of the Study:  The logistic regression model and the desirability function are adequate chemometric techniques to improve the properties of the methods, because it is possible to increase sensitivity and decrease cross‐specificity simultaneously. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5437726 Mon, 24 Oct 2011 04:00:00 +0100 5437726 http://www.medworm.com/index.php?rid=5356029&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03167.x Conclusions:  Oxacillin resistance screening agar base (Oxoid) and Brilliance MRSA agar (Oxoid) are most suitable for the detection and isolation of MRSP from clinical material.Significance and Impact of the Study:  The importance of MRSP in veterinary medicine is increasing. Diagnostic systems are needed to detect MRSP carrier as soon as possible. This study provides a selection of screening agars for the detection of MRSP to the clinical microbiologists. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5356029 Mon, 24 Oct 2011 04:00:00 +0100 5356029 http://www.medworm.com/index.php?rid=5356028&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03168.x Conclusions:  Several CPPs, while being non‐toxic to mammalian cells, possess growth inhibitory activity on the very stringent yeast M. sympodialis.Significance and impact of study:  Our finding that Five CPPs and one AMP that are harmless towards mammalian cells act as antifungal agents against M. sympodialis opens up the possibility to use these in treatment of AE, SE and dandruff. To our knowledge, this is the first time peptides have been identified as antifungal agents against M. sympodialis. Further studies to elucidate the mechanism are warranted. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5356028 Mon, 24 Oct 2011 04:00:00 +0100 5356028 http://www.medworm.com/index.php?rid=5344030&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03164.x Conclusions:  Localizations of GAPDHs from these pathogenic bacteria were similar to many other causative agents. And GAPDHs could be important protective antigens and give remarkable cross immunity against different pathogens.Significance and Impact of the Study:  Recombinant GAPDH could be designed as a broad spectrum vaccine candidate against multiple microbial infections in aquaculture. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5344030 Thu, 20 Oct 2011 04:00:00 +0100 5344030 http://www.medworm.com/index.php?rid=5344029&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03165.x Conclusions:  The results indicated that cellobiose (5%) and D–galactose (5%) gave maximum viability of strains Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 (98% and 54.3% respectively) while the perfect one (100%) of strain Bacillus cereus AR156 was obtained with sucrose (5%) during freeze‐drying, and the highest survival of the three strains was reached when they were rehydrated with 10% non‐fat skim milk. In the following storage, the survival rates showed that Bacillus cereus AR156 could still reach 50% after 12 months.Significance and Impact of the study: This study showed that freeze drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale‐up possibilities and formulation development. (Source: Lett... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5344029 Thu, 20 Oct 2011 04:00:00 +0100 5344029 http://www.medworm.com/index.php?rid=5321930&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03163.x Conclusions:  European starlings may be a suitable reservoir and vector of E. coli O157:H7.Significance and Impact of Study:  Given the duration and magnitude of E. coli O157:H7 shedding by European starlings, European starlings should be considered a public health hazard. Measures aimed at controlling environmental contamination with starling excrement, on the farm and in public venues, may decrease food‐producing animal and human exposure to this pathogen. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5321930 Sun, 16 Oct 2011 17:11:29 +0100 5321930 http://www.medworm.com/index.php?rid=5376549&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03157.x Conclusions:  Fungal and mycotoxin contamination in malt and brewer’s grain is an actual risk for animal and human health.Significance and Impact of the Study:  This study may be useful for assessing the risk of mycotoxins in Argentinean beers and especially in animal feeds. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5376549 Tue, 04 Oct 2011 04:00:00 +0100 5376549 http://www.medworm.com/index.php?rid=5282086&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03157.x Conclusions:  fungal and mycotoxin contamination in malt and brewer’s grain is an actual risk for animal and human health. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282086 Tue, 04 Oct 2011 04:00:00 +0100 5282086 http://www.medworm.com/index.php?rid=5282085&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03158.x Conclusions:  The pyrosequencing analysis of the 16S rDNA V1 is a useful molecular tool for rapid identification of suspected isolates of Francisella sp. in clinical or environmental samples. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282085 Tue, 04 Oct 2011 04:00:00 +0100 5282085 http://www.medworm.com/index.php?rid=5282084&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03159.x Conclusion:  Enriched electrochemically‐active bacteria originated from POME demonstrated potential to generate bioenergy from both acetate and complex POME substrates. Further improvements include development of MFC systems which are able to utilize both fermentative and non‐fermentative substrates in POME is needed to maximize the bioenergy generation. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282084 Tue, 04 Oct 2011 04:00:00 +0100 5282084 http://www.medworm.com/index.php?rid=5282083&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03160.x AbstractThe aim of this study was to establish a novel cell surface display system that would enable the display of target proteins on Lactobacillus plantarum. BLASTP analysis of the amino acids sequence data revealed that the N‐terminus of the putative muropeptidase MurO from Lact. plantarum contained two putative lysin motif (LysM) repeat regions, implying that the MurO was involved in bacterial cell wall binding. To investigate the potential of MurO for surface display, green fluorescent protein (GFP) was fused to MurO at its C‐terminus and the resulting fusion protein was expressed in Escherichia coli. After being mixed with Lact. plantarum cells in vitro, GFP was successfully displayed on the surfaces of Lact. plantarum cells. Increases in the fluorescence intensities of chemicall... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282083 Tue, 04 Oct 2011 04:00:00 +0100 5282083 http://www.medworm.com/index.php?rid=5302080&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03162.x Conclusions:  It may be advisable to use bleach solutions with lower pH (rather than high FAC levels) in light of both the decontamination efficacy and bleach stability results. For wood materials, use of sporicides other than acidified bleach may be warranted.Significance and Impact of the Study:  These results may be useful in preparing acidified bleach solutions for decontamination of materials contaminated with spores such as B. anthracis. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5302080 Sat, 01 Oct 2011 04:00:00 +0100 5302080 http://www.medworm.com/index.php?rid=5282082&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03161.x Conclusions:  The simple extraction method for enumerating Enterococcus in beach sands was easily transferable to and repeatable among laboratories with little or no prior experience. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5282082 Sat, 01 Oct 2011 04:00:00 +0100 5282082 http://www.medworm.com/index.php?rid=5269762&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03156.x Conclusions:  The treatment of wet environments in the presence of human activity such as kitchens, toilets, and bathrooms with the low‐concentration ClO2 gas would be useful for reducing the risk of infection by bacteria and viruses residing on the environmental hard surfaces without adverse effects.Significance and Impact of the Study:  This study demonstrates that the low‐concentration ClO2 gas (mean 0·05 ppmv) inactivate various kinds of microbes such as Gram‐positive and Gram‐negative bacteria, enveloped and non‐enveloped viruses in the wet state. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5269762 Sat, 01 Oct 2011 01:24:53 +0100 5269762 http://www.medworm.com/index.php?rid=5331375&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03156.x Conclusions:  The treatment of wet environments in the presence of human activity such as kitchens and bathrooms with the low‐concentration ClO2 gas would be useful for reducing the risk of infection by bacteria and viruses residing on the environmental hard surfaces without adverse effects.Significance and Impact of the Study:  This study demonstrates that the low‐concentration ClO2 gas (mean 0·05 ppmv) inactivates various kinds of microbes such as Gram‐positive and Gram‐negative bacteria, enveloped and nonenveloped viruses in the wet state. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331375 Wed, 28 Sep 2011 04:00:00 +0100 5331375 http://www.medworm.com/index.php?rid=5257705&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03155.x Conclusions:  The microbiota of all narezushi products were shown to consist largely of Lactobacillales populations. Interestingly, different species were found to be dominant in each product.Significance and Impact of the Study:  This study provides an insight into the bacterial composition of fermented fish‐based foods, which are consumed worldwide. Significant differences in the dominant species were observed between products, possibly because of the starter‐free production process. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5257705 Tue, 27 Sep 2011 09:16:27 +0100 5257705 http://www.medworm.com/index.php?rid=5331376&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03155.x Conclusions:  The microbiota of all narezushi products were shown to consist largely of Lactobacillales populations. Interestingly, different species were found to be dominant in each product.Significance and Impact of the Study:  This study provides an insight into the bacterial composition of fermented fish‐based foods, which are consumed worldwide. Significant differences in the dominant species were observed between products, possibly because of the starter‐free production process. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5331376 Tue, 27 Sep 2011 04:00:00 +0100 5331376 http://www.medworm.com/index.php?rid=5246925&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03154.x Conclusions: B. glumae AU6208 can produce considerable amounts of rhamnolipids. They are produced as diversified mixtures of congeners. Their side chains are longer than those normally produced by those of Pseudomonas aeruginosa. They also present excellent tensioactive properties. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246925 Fri, 23 Sep 2011 15:10:54 +0100 5246925 http://www.medworm.com/index.php?rid=5234273&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03151.x Conclusions:  The isolate HGR‐25 was identified as Caulobacter isolated from the plants growing in soil samples collected from Khareemnagar district, Andhra Pradesh, India. Inoculation tests revealed that Caulobacter formed nodules on Horse gram. It was also confirmed by Ribosomal Database Project. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234273 Tue, 20 Sep 2011 05:09:37 +0100 5234273 http://www.medworm.com/index.php?rid=5246927&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03152.x Conclusion:  Significant contamination of HAV in edible bivalve shellfish was observed. Beside digestive tissue, gills are one of the important samples for viral genome detection. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246927 Tue, 20 Sep 2011 04:00:00 +0100 5246927 http://www.medworm.com/index.php?rid=5246926&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03153.x Conclusions:  Results indicate that most Lactococcus garvieae isolates from pigs and trout exhibited a distinct genetic background. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246926 Tue, 20 Sep 2011 04:00:00 +0100 5246926 http://www.medworm.com/index.php?rid=5234276&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03138.x (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234276 Mon, 19 Sep 2011 04:00:00 +0100 5234276 http://www.medworm.com/index.php?rid=5218235&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03150.x Conclusions:  The studies showed that R. aquatilis was resistant to freezing and freeze‐drying when it was used a mixture of cryoprotectants, and that it was possible to obtain inoculums with high viability and good effectiveness for reduction of decay caused by Botrytis cinerea (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5218235 Wed, 14 Sep 2011 11:23:48 +0100 5218235 http://www.medworm.com/index.php?rid=5204863&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03149.x Conclusions:  The MIC values were 4.7 mmol kg−1 for P. expansum, 8.2 and 7.3 mmol kg−1 for B. cinerea strain BC1 and BC2 respectively, demonstrating that some isolates of these moulds are resistant to copper. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204863 Sun, 11 Sep 2011 05:14:45 +0100 5204863 http://www.medworm.com/index.php?rid=5269764&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03147.x Conclusions:  The rdar or bdar morphotype may influence S. Sofia persistence but did not explain why bdar strains predominate in this serotype.Significance and Impact of the Study:  This work provides some insight into why some Salmonella strains survive in poultry environments and may ultimately contribute to their control. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5269764 Thu, 08 Sep 2011 04:00:00 +0100 5269764 http://www.medworm.com/index.php?rid=5269763&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03149.x Conclusions:  The MIC values were 4·7 mmol kg−1 for P. expansum, 8·2 and 7·3 mmol kg−1 for B. cinerea strain BC1 and BC2, respectively, demonstrating that some isolates of these moulds are resistant to copper.Significance and Impact of the Study:  Copper concentrations at 4 mmol kg−1 would be sufficient to control the development of these isolates, but the toxicity of copper should be extended to other isolates and evaluated in vineyards. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5269763 Thu, 08 Sep 2011 04:00:00 +0100 5269763 http://www.medworm.com/index.php?rid=5204865&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03147.x Conclusions:  The rdar or bdar morphotype may influence S. Sofia persistence but did not explain why bdar strains predominate in this serotype. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204865 Thu, 08 Sep 2011 04:00:00 +0100 5204865 http://www.medworm.com/index.php?rid=5204864&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03148.x Conclusions:  The results obtained indicate that the hygienic quality of DP is for almost all microbiological parameters better than that of the bovine manure (range of reduction 1,6‐3,1 log10) and suggest the need to identify specific pathogen indicators related to the hygienic characteristics of digestate products. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5204864 Thu, 08 Sep 2011 04:00:00 +0100 5204864 http://www.medworm.com/index.php?rid=5191679&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03129.x (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5191679 Thu, 01 Sep 2011 23:00:00 +0100 5191679 http://www.medworm.com/index.php?rid=5194825&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03146.x Conclusion:  These data shows that D. elongata have antiviral activity mainly against HSV‐1 and DENV‐2, besides antioxidant activity and it has been principally attributed to flavonoids isolated. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5194825 Wed, 31 Aug 2011 23:00:00 +0100 5194825 http://www.medworm.com/index.php?rid=5191676&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03145.x Conclusion:  Disc susceptibility testing methods are unreliable on detecting colistin resistance. MIC should be determined to confirm the susceptibility results by disc diffusion. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5191676 Wed, 31 Aug 2011 23:00:00 +0100 5191676 http://www.medworm.com/index.php?rid=5246928&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03143.x Conclusions:  TEMPO methods were equivalent to the corresponding standard plate methods with very good rates of agreement.Significance and Impact of the Study:  The automated MPN method is more practical and reliable for in‐house microbiological testing in processed soy products. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5246928 Wed, 31 Aug 2011 04:00:00 +0100 5246928 http://www.medworm.com/index.php?rid=5191678&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03143.x Conclusions:  TEMPO methods were equivalent to the corresponding standard plate methods with very good rates of agreement. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5191678 Tue, 30 Aug 2011 23:00:00 +0100 5191678 http://www.medworm.com/index.php?rid=5191677&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03144.x Conclusions:  MRTC primers displayed significantly improved etnC quantitation in both pure cultures and environmental samples. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5191677 Tue, 30 Aug 2011 23:00:00 +0100 5191677 http://www.medworm.com/index.php?rid=5174129&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03141.x Conclusions:  The MTBC‐LAMP is a useful assay for the diagnosis of tuberculous pleurisy, especially in pleural effusion smear/culture‐negative patients. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5174129 Tue, 30 Aug 2011 03:24:31 +0100 5174129 http://www.medworm.com/index.php?rid=5234275&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03140.x Conclusions:  Our strategy enabled the selection of improved yeasts with desired oenological characteristics. Particularly, resistance to toxic analogues of sulfate allowed us to detect strains that unable to assimilate sulfates.Significance and Impact of the Study:  This strategy that combines the sexual recombination of spores and application of a specific selective pressure provides a rapid screening method to generate genetic variants and select improved wine yeast strains with an impaired metabolism regarding the production of sulfites and H2S. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234275 Mon, 29 Aug 2011 04:00:00 +0100 5234275 http://www.medworm.com/index.php?rid=5234274&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03141.x Conclusions:  The MTBC–LAMP is a useful assay for the diagnosis of tuberculous pleurisy, especially in pleural effusion smear‐/culture‐negative patients.Significance and Impact of the Study:  Tuberculous pleural effusion usually contains low number of mycobacteria, which leads to low diagnostic sensitivity of acid‐fast staining and mycobacterial culture methods. In this study, we developed a simple and sensitive LAMP assay for the diagnosis of tuberculous pleurisy. This assay should have broad application in resource‐limited settings. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234274 Mon, 29 Aug 2011 04:00:00 +0100 5234274 http://www.medworm.com/index.php?rid=5174132&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03142.x Conclusions:  New safety criteria, along with hygienic control measures and consumer education strategies are essential to improve the microbiological safety of infant or baby foods. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5174132 Sun, 28 Aug 2011 23:00:00 +0100 5174132 http://www.medworm.com/index.php?rid=5174131&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03139.x Conclusions:  The permanent assessment of Pseudomonas aeruginosa is necessary not only in water, as expressed in official programs, but also in equipments where biofilms are evident. Pseudomonas aeruginosa was more prevalent in biofilm populations and presented higher ability to adapt to antibiotic pressure. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5174131 Sun, 28 Aug 2011 23:00:00 +0100 5174131 http://www.medworm.com/index.php?rid=5174130&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03140.x Conclusions:  Our strategy enabled the selection of improved yeasts with desired oenological characteristics. Particularly resistance to toxic analogues of sulfate allowed to detect strains unable to assimilate sulfates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5174130 Sun, 28 Aug 2011 23:00:00 +0100 5174130 http://www.medworm.com/index.php?rid=5156617&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03137.x Conclusions:  The 70 DH % PKE peptides exhibited greatest overall antibacterial effect of the various peptides of PKE evaluated. Further research is needed to determine the mode of action of PKE peptides.Significance and Impact of the Study:  PKE peptides, a natural plant product, effectively inhibited the growth of spore‐forming and non‐sporeforming Gram‐positive bacteria. Potentially PKE peptides could be used in food preservation and developed as antibacterial agent in the pharmaceutical industry. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5156617 Thu, 25 Aug 2011 10:15:34 +0100 5156617 http://www.medworm.com/index.php?rid=5234277&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03137.x Conclusions:  The 70 DH % PKE peptides exhibited greatest overall antibacterial effect of the various peptides of PKE evaluated. Further research is needed to determine the mode of action of PKE peptides.Significance and Impact of the Study:  Palm kernel expeller peptides, a natural plant product, effectively inhibited the growth of spore‐forming and non‐spore‐forming Gram‐positive bacteria. Potentially, PKE peptides could be used in food preservation and developed as antibacterial agent in the pharmaceutical industry. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5234277 Wed, 17 Aug 2011 04:00:00 +0100 5234277 http://www.medworm.com/index.php?rid=5156618&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03136.x Conclusion:  Strain L. plantarum AS1 inhibits the cell attachment of a pathogen V. parahaemolyticus by steric hindrance mechanism. Also, antibacterial factors such as bacteriocins, lactic acid and exopolysaccharides could be involved.Significance and Impact of the Study:  The ability to inhibit the adhesion of V. parahaemolyticus to intestinal cell line warrants further investigation to explore the use of probiotic strain L. plantarum AS1 in the management of gastroenteritis caused due to V. parahaemolyticus. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5156618 Mon, 15 Aug 2011 23:00:00 +0100 5156618 http://www.medworm.com/index.php?rid=5124689&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03134.x Conclusions:  Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5124689 Thu, 11 Aug 2011 23:00:00 +0100 5124689 http://www.medworm.com/index.php?rid=5124688&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03135.x Conclusions:  The newly designed [FeFe] hydrogenase‐cluster specific primers, targeting the cluster‐conserved regions, allow for a direct amplification of a specific hydrogenase gene from the species of interest. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5124688 Sun, 31 Jul 2011 23:00:00 +0100 5124688 http://www.medworm.com/index.php?rid=5094681&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03133.x Conclusions:  Of the concentrations tested, 50 mg l−1 cycloheximide inhibited many grape/wine yeasts yet generally yielded countable colonies of Dekkera (1 to 2.5 mm diameter). Several strains of Dekkera did not grow well on DBDM, probably due to the lack of an unidentified nutrient(s).Significance and Impact of the Study:  Better media formulations will improve detection of Dekkera, thereby increasing microbiological control during winemaking. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5094681 Sun, 31 Jul 2011 23:00:00 +0100 5094681 http://www.medworm.com/index.php?rid=5085459&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03132.x Conclusion:  The results have shown that oral Lactobacillus SD1‐SD6 showed a strong inhibitory effect against S. mutans and Streptococcus sobrinus, as well as, gram‐negative periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5085459 Sun, 31 Jul 2011 23:00:00 +0100 5085459 http://www.medworm.com/index.php?rid=5085461&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03130.x Conclusions:  Assimilation tests alone are unreliable as a universal means of yeast identification, due to numerous new species, variability of strains, and increasing coincidence of assimilation profiles. Assimilation tests still have a useful role in identification of common species, such as the majority of clinical isolates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5085461 Fri, 29 Jul 2011 23:00:00 +0100 5085461 http://www.medworm.com/index.php?rid=5085460&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03131.x Conclusions:  This strategy, utilizing streptavidin‐displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson’s disease. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5085460 Fri, 29 Jul 2011 23:00:00 +0100 5085460 http://www.medworm.com/index.php?rid=5061657&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03128.x Conclusions:  We have successfully developed the time‐dependent dose‐response models to describe mortality of animals exposed to an HPAI H5N1 virus. The developed model describes the mortality over time and represents observed experimental responses accurately. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5061657 Mon, 25 Jul 2011 20:06:56 +0100 5061657 http://www.medworm.com/index.php?rid=5169188&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03128.x Conclusions:  We have successfully developed the time‐dependent dose–response models to describe the mortality of animals exposed to an HPAI H5N1 virus. The developed model describes the mortality over time and represents observed experimental responses accurately.Significance and Impact of the Study:  This is the first study describing time‐dependent dose–response models for HPAI H5N1 virus. The developed models will be a useful tool for estimating the mortality of HPAI H5N1 virus, which may depend on time postexposure, for the preparation of a future influenza pandemic caused by this lethal virus. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5169188 Sun, 24 Jul 2011 23:00:00 +0100 5169188 http://www.medworm.com/index.php?rid=5057442&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03127.x Conclusions:  All Pyrenophora teres f. teres isolates were differentially pathogenic. CI09214 and CI05401 cultivars were released as the most effective sources of resistance in Syria and Tunisia. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5057442 Sun, 24 Jul 2011 05:24:11 +0100 5057442 http://www.medworm.com/index.php?rid=5048845&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03126.x Conclusions:  ΔaroCΔeseBCDΔesaC behaved a low virulence and the highest RPS on zebra fish model. When the zebra fish were vaccinated with ΔaroCΔeseBCDΔesaC via injection, the expression of immune‐related factors including IgM and MHC II were up‐regulated.Significance and Impact:  The mutant ΔaroCΔeseBCDΔesaC might serve as an effective live attenuated vaccine against edwardsiellosis. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048845 Fri, 22 Jul 2011 00:38:03 +0100 5048845 http://www.medworm.com/index.php?rid=5156619&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03125.x Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry‐heat treatment should be investigated, and conditions for drying and dry‐heat treatment should be optimized.Significance and Impact of the study:  This study showed that sequential treatment with ClO2 and dry‐heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5156619 Mon, 18 Jul 2011 23:00:00 +0100 5156619 http://www.medworm.com/index.php?rid=5048847&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03125.x Conclusions: E. coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry heat treatment should be investigated, and conditions for drying and dry heat treatment should be optimized. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048847 Mon, 18 Jul 2011 23:00:00 +0100 5048847 http://www.medworm.com/index.php?rid=5048846&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03124.x AbstractMicrobial contamination is a pervasive problem in any ethanol fermentation system. These infections can at minimum affect the efficiency of the fermentation and at their worse lead to stuck fermentations causing plants to shut down for cleaning before beginning anew. These delays can result in costly loss of time as well as lead to an increased cost of the final product. Lactic acid bacteria (LAB) are the most common bacterial contaminants found in ethanol production facilities and have been linked to decreased ethanol production during fermentation. Lactobacillus sp generally predominant as these bacteria are well adapted for survival under high ethanol, low pH and low oxygen conditions found during fermentation. It has been generally accepted that lactobacilli cause inhibition of... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048846 Mon, 18 Jul 2011 23:00:00 +0100 5048846 http://www.medworm.com/index.php?rid=5106205&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03122.x Conclusions:  These data showed the strong killing effect of cinnamaldehyde against MRSA within biofilms.Significance and Impact of the Study:  This study indicated the potential of cinnamaldehyde as an inhibitory agent for use in MRSA biofilm‐related infections. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5106205 Sun, 17 Jul 2011 23:00:00 +0100 5106205 http://www.medworm.com/index.php?rid=5094682&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03123.x Conclusions:  A method was developed that is sensitive, rapid and reliable for detecting defluorination by micro‐organisms growing on solidified medium.Significance and Impact of the Study:  This method can be used to facilitate the isolation of micro‐organisms capable of defluorination. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5094682 Sun, 17 Jul 2011 23:00:00 +0100 5094682 http://www.medworm.com/index.php?rid=5048850&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03121.x SummaryIt has been known for decades that poultry meat is the most common single source for campylobacteriosis, and yet, the problem has not been solved. This review identifies some of the reasons why our attempts to reduce the incidence of this pathogen have largely failed. Based on the literature, the events a virtual population of Campylobacter may encounter, from growing in the gut of a broiler to eventually infecting humans and causing disease, are reviewed. Most steps in the farm‐to‐fork process are well studied, though there are gaps in our knowledge about survival and spread of Campylobacter populations before they enter the farm. Key events in the farm‐to‐fork chain that are suitable targets for prevention and control, in order to reduce food‐born campylobacteriosis, are... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048850 Sun, 17 Jul 2011 23:00:00 +0100 5048850 http://www.medworm.com/index.php?rid=5048849&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03122.x Conclusions:  These data showed the strong killing effect of cinnamaldehyde against MRSA within biofilms. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048849 Sun, 17 Jul 2011 23:00:00 +0100 5048849 http://www.medworm.com/index.php?rid=5048848&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03123.x Conclusions:  A method was developed that is sensitive, rapid and reliable for detecting defluorination by microorganisms growing on solidified medium. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048848 Sun, 17 Jul 2011 23:00:00 +0100 5048848 http://www.medworm.com/index.php?rid=5018542&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03120.x Conclusions:  The wine trials indicated that L. brevis IOEB 9809 is able to produce both tyramine and putrescine during wine fermentation. Increased cell viability was also observed in wine supplemented with tyrosine or agmatine. qRT‐PCR analysis suggests a strong influence of substrate availability on the expression of genes coding for tyrosine decarboxylase and agmatine deiminase in L. brevis IOEB 9809. Less evident is the relationship between putrescine and tyramine production and tolerance to wine pH.Significance and Impact of Study:  To our knowledge, this study represents the first assessment of relative expression of L. brevis IOEB 9809 genes involved in biogenic amine production in wine. Furthermore, an effect of biogenic amine production on viability of L. brevis during wine... Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5018542 Tue, 12 Jul 2011 21:40:52 +0100 5018542 http://www.medworm.com/index.php?rid=5094683&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03117.x Conclusions:  The multiplex PCR assay was a cost effective, quick diagnostic technique, which was helpful in differentiating TMV and ToMV accurately.Significance and Impact of the Study:  The multiplex PCR assay described in this study is a valuable tool for plant pathology and basic research studies. This method may facilitate better recognition and distinction of TMV and ToMV in both pepper and tomato. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5094683 Fri, 08 Jul 2011 23:00:00 +0100 5094683 http://www.medworm.com/index.php?rid=5076848&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03118.x Conclusions:  Use of a conditioning liquid characterized by acidity and a correct storage temperature is able to counteract pathogen replication during shelf life. A high concentration of lactic acid bacteria was associated with effective control of L. monocytogenes but the role of lactic acid bacteria in WBMC conditioning liquid requires further investigation.Significance and Impact of the Study:  According to European regulations, food producers should be able to justify decision‐making on the shelf life assigned to their products, taking into account reasonable storage conditions and use by consumers. The results of the trial yielded information for producers of WBMC and similar cheeses for decision‐making on product shelf life. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5076848 Fri, 08 Jul 2011 23:00:00 +0100 5076848 http://www.medworm.com/index.php?rid=5018545&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03117.x Conclusions:  The multiplex PCR assay was a cost effective, quick diagnostic technique which was helpful in differentiating TMV and ToMV accurately.Significance and Impact of the Study:  The multiplex PCR assay described in this study are valuable tools for plant pathology and basic research studies. This method may facilitate better recognition and distinction of TMV and ToMV in both pepper and tomato. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5018545 Fri, 08 Jul 2011 23:00:00 +0100 5018545 http://www.medworm.com/index.php?rid=5018544&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03118.x Conclusions:  Use of a conditioning liquid characterised by acidity and a correct storage temperature is able to counteract pathogen replication during shelf life. A high concentration of lactic acid bacteria was associated with effective control of L. monocytogenes but the role of lactic acid bacteria in WBMC conditioning liquid requires further investigation.Significance and impact of the study:  According to European Regulations, food producers should be able to justify decision‐making on the shelf life assigned to their products, taking into account reasonable storage conditions and use by consumers. The results of the trial yielded information for producers of WBMC and similar cheeses for decision‐making on product shelf life. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5018544 Fri, 08 Jul 2011 23:00:00 +0100 5018544 http://www.medworm.com/index.php?rid=5018543&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03119.x Conclusions:  This is the first report demonstrating a high percentage of potential pathogenic V. parahaemolyticus trh+ strains in estuarine fishes of the Mediterranean area.Significance and Impact of the Study:  These findings indicate the potential human health risk associated with the presence of pathogenic Vibrio spp. in wild fishes. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5018543 Fri, 08 Jul 2011 23:00:00 +0100 5018543 http://www.medworm.com/index.php?rid=5007071&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03116.x Conclusions:  The results of the study indicate that the melanin produced by the newly isolated A. bridgeri strain is a member of DHN melanin family and exhibited significant free radical scavenging activity.Significance and Impact of the Study:  This is the first report on characterization of DHN‐melanin produced by a novel strain of A. bridgeri and may find potential application as a natural antioxidant in the cosmetic and pharmaceutical industries. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5007071 Fri, 08 Jul 2011 14:19:47 +0100 5007071 http://www.medworm.com/index.php?rid=5007072&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03115.x Conclusion:  A high level of genetic diversity was observed which indicates that the Kolli Hills’C. violaceum isolates would fall into at least three new clusters.Significance and Impact of the Study:  The results showed a noteworthy bacterial variation and genetic diversity of C. violaceum in the unexplored virgin forest area. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5007072 Mon, 04 Jul 2011 23:00:00 +0100 5007072 http://www.medworm.com/index.php?rid=5048851&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03113.x Conclusions:  The prevalence of E. coli O157: H7 is substantial from two abattoirs in the country. The carriage and shedding of E. coli O157: H7 did not differ with season but differed among groups of ruminants and among breeds of cattle in a tropical country.Significance and Impact of the Study:  This is the first study on E. coli O157: H7 from abattoir operations in Nigeria. The study emphasizes the risk of E. coli O157: H7 along the meat chain and the need for concerted effort to limit it through best hygiene practices. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5048851 Thu, 30 Jun 2011 23:00:00 +0100 5048851 http://www.medworm.com/index.php?rid=4992063&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03114.x Conclusions:  The described methods were specific, rapid and simple to perform and interpret.Significance and Impact of the Study:  The developed genotyping assays provide a valuable tool for identification and differentiation of specific BT2 strains of Y. ruckeri. These assays will be critical for the design and validation new vaccines or other measures meant to control BT2 strains. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4992063 Thu, 30 Jun 2011 23:00:00 +0100 4992063 http://www.medworm.com/index.php?rid=4992062&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03113.x Conclusions:  The prevalence of E. coli O157: H7 is substantial from two abattoirs in the country. The carriage and shedding of E. coli O157: H7 did not differ with season but differed among groups of ruminants and among breeds of cattle in a tropical country.Significance and Impact of the Study:  This is the first study on E. coli O157: H7 from abattoir operations in Nigeria. The study emphasizes the risk of E. coli O157: H7 along the meat chain and the need for concerted effort to limit it through best hygiene practices. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4992062 Thu, 30 Jun 2011 23:00:00 +0100 4992062 http://www.medworm.com/index.php?rid=4983879&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03112.x Conclusion:  The concentration of a large volume of water (up to one liter) on a filter membrane avoids false negative results obtained by direct analysis as it allows to detect low number of bacteriophages in water samples.Significance and Impact of the Study:  The addition of concentration step before applying the standard method could be useful to enhance the reliability of bacteriophages monitoring in water samples as bio‐indicators to highlight fecal pollution. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4983879 Thu, 30 Jun 2011 14:28:20 +0100 4983879 http://www.medworm.com/index.php?rid=4983881&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03110.x Conclusions:  The study indicated a general antimicrobial effect of lead on oral bacterial species in the range of 0.15‐10 mmol l−1. The toxicity of silver nitrate was the highest, whereas that of ferrous sulphate was the lowest. Gram‐positive species had a tendency to be less susceptible for metals than Gram‐negatives.Significance and Impact of the Study:  The study shows that it is possible that microbiological changes may occur in the dental plaque in children due to toxic exposure of environmental lead. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4983881 Tue, 28 Jun 2011 23:00:00 +0100 4983881 http://www.medworm.com/index.php?rid=4983880&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03111.x Conclusions:  We demonstrated that the disruption of CAC2634 in C. acetobutylicum enables its electrotransformation in air.Significance and Impact of the Study:  The electrotransformation process of C. acetobutylicum could be significantly simplified, especially when operating multiple electrotransformations. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4983880 Tue, 28 Jun 2011 23:00:00 +0100 4983880 http://www.medworm.com/index.php?rid=4974995&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03109.x Conclusions:  The results of the study suggest that the high prevalence of carbapenem resistance among Acinetobacter baumannii organisms in this institution is not caused by the spread of a predominant clone.Significance and Impact of the Study:  This work reinforces the importance monitoring antimicrobial susceptibility rates. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4974995 Tue, 28 Jun 2011 20:08:44 +0100 4974995 http://www.medworm.com/index.php?rid=4967928&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03108.x Conclusions:  Our result suggested that recA sequencing is a reliable analysis method for understanding the relatedness of the local isolates with the isolates obtained elsewhere.Significance and Impact of Study:  Understanding the genetic relatedness between V.cholerae isolates could give insights to the health care system for better control and prevention of the cholera. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4967928 Sun, 26 Jun 2011 17:48:18 +0100 4967928 http://www.medworm.com/index.php?rid=4967929&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03107.x Conclusion:  In some cases, influenza and rhinovirus RNA can be detected on aircraft air filters, even ten days after the filters were removed from aircraft.Significance and Impact of the Study:  With protocol modifications to overcome PCR inhibition, air filter sampling and the ResPlex II assay could be used to characterize viruses in aircraft cabin air. Information about viruses in aircraft could support public health measures to reduce disease transmission within aircraft and between cities. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4967929 Fri, 24 Jun 2011 23:00:00 +0100 4967929 http://www.medworm.com/index.php?rid=4967931&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03105.x Conclusions:  geriatric cats harboured a complex faecal microbiota and ∼41% of total bacteria have been detected with the probes employed.Significance and impact of study:  First molecular‐based study examining faecal microbiota of geriatric felines. Knowledge of the microbiota associated with ageing in cats may allow improved development of foods specific for the needs of senior cats. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4967931 Wed, 22 Jun 2011 23:00:00 +0100 4967931 http://www.medworm.com/index.php?rid=4967930&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03106.x Conclusions:  This study showed high antibiotic resistance of the Salmonella strains investigated and the presence of multidrug‐resistant Salm. Typhimurium DT104 in infections of Portuguese children.Significance and Impact of Study:  Regarding the increase of antibiotic resistance by Salmonella strains isolated from infections in Portuguese children, and the presence of Salm. Typhimurium DT 104 circulating in Portugal. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4967930 Wed, 22 Jun 2011 23:00:00 +0100 4967930 http://www.medworm.com/index.php?rid=4951717&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03104.x Conclusions:  This is the first report of NoV GGII.4 in Albania causing an outbreak. The genetic analysis showed several point mutations and amino‐acid substitutions respect to the international strains.Significance and Impact of Study:  Over the last decades, Albania has suffered from different outbreaks as cholera, poliomyelitis, hepatitis A and now, for the first time, it has been documented an outbreak of NoV. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4951717 Tue, 21 Jun 2011 17:55:57 +0100 4951717 http://www.medworm.com/index.php?rid=4997202&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03104.x Conclusions:  This is the first report of NoV GGII·4 in Albania causing an outbreak. The genetic analysis showed several point mutations and amino acid substitutions with respect to the international strains.Significance and Impact of Study:  Over the last decades, Albania has suffered from different outbreaks as cholera, poliomyelitis, hepatitis A and now, for the first time, it has been documented an outbreak of NoV. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4997202 Sun, 19 Jun 2011 23:00:00 +0100 4997202 http://www.medworm.com/index.php?rid=4932863&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03103.x Conclusions: E. tarda strains exhibited diversities in phenotypic characteristicswhich may be linked to differences in geographic location or host origin. In addition, the number of flagella is essential for bacterial motility and biofilm formation.Significance and impact of the study:  This is the first report demonstrating the difference in flagella formation between E. tarda strains, which may broaden the understanding of flagellation trait at intra‐species level. Further‐more, evaluation of virulence associated characteristics can provide useful information for unveiling the diverse pathogenic mechanisms of E. tarda. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4932863 Thu, 16 Jun 2011 22:40:20 +0100 4932863 http://www.medworm.com/index.php?rid=5007073&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03100.x Conclusions:  The developed colony hybridization system allows for the efficient and simultaneous isolation of all DEC pathotypes.Significance and Impact of the Study:  The colony hybridization system described here permits the sensitive isolation of DEC and represents a suitable tool for ecological investigations of DEC. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=5007073 Wed, 15 Jun 2011 23:00:00 +0100 5007073 http://www.medworm.com/index.php?rid=4932866&cid=s_32054_77_f&fid=32054&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1472-765X.2011.03100.x Conclusions:  The developed colony hybridization system allows for the efficient and simultaneous isolation of all DEC pathotypes.Significance and Impact of the Study:  The colony hybridization system described here permits the sensitive isolation of DEC and represents a suitable tool for ecological investigations of DEC. (Source: Letters in Applied Microbiology) Letters in Applied Microbiology journals http://www.medworm.com/rss/comments.php?id=4932866 Wed, 15 Jun 2011 23:00:00 +0100 4932866