MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Medical Molecular Morphology' source. Thu, 09 Feb 2012 16:56:49 +0100 http://www.medworm.com/index.php?rid=5520332&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy4v32q520356w078%2F This study revealed that PRP promotes epithelialization and angiogenesis of split-thickness skin graft donor sites. Content Type Journal ArticleCategory Case ReportPages 233-236DOI 10.1007/s00795-010-0532-1Authors Natsuko Kakudo, Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, 570-8506 JapanSatoshi Kushida, Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, 570-8506 JapanTatsuya Minakata, Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, 570-8506 JapanKenji Suzuki, Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, 570-8506 JapanKenji Kusumoto, Department of Plastic and... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520332 Fri, 16 Dec 2011 16:47:46 +0100 5520332 http://www.medworm.com/index.php?rid=5520333&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F771p0n2520823t21%2F We report here that basal keratinocytes showed only weak positivity of CYP26A1 in sunlight-nonexposed areas, whereas strong positive staining was observed in skin from chronically sunexposed body areas and in epidermis that had the dysplastic changes known as actinic keratosis. However, we found no expression of constitutive CYP26A1 in skin malignancies such as squamous cell carcinomas. Our observation suggests an involvement of enhanced CYP26A1 expression causing a functional VAD state in skin that can potentially lead to neoplastic transformation of keratinocytes in an early phase during skin carcinogenesis. Content Type Journal ArticleCategory Original PaperPages 200-206DOI 10.1007/s00795-010-0528-xAuthors Makoto Osanai, Department of Pathology, Kochi University School of Me... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520333 Fri, 16 Dec 2011 16:47:32 +0100 5520333 http://www.medworm.com/index.php?rid=5520336&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe421557027864253%2F We examined the morphological changes in fibers, localization of apoptotic cells, and protein expression of αB-crystallin in the lens of Morioka cataract (MCT) mice, a novel cataract model. Using a scanning electron microscope, swollen lens fibers and enlarged spaces between lens fibers were observed in the lens of 3-week-old MCT mice. At 2 weeks of age (before cataract), the single-strand DNA (ssDNA)-positive (indicating apoptosis) cell ratio of the lens epithelium was significantly higher in MCT than in wild-type ddY mice. At 2 and 4 weeks of age, αB-crystallin protein expression of the lens in MCT mice was significantly lower than that in wild-type ddY mice. These findings suggest that increase in apoptosis and reduction in αBcrystallin level are involved in the cataractogenesi... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520336 Fri, 16 Dec 2011 16:47:30 +0100 5520336 http://www.medworm.com/index.php?rid=5520335&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn5v4158578m0l328%2F In conclusion, the numerical abnormalities of chromosomes 3 and 16, irrespective of gain or loss, may be characteristic of ACD-associated RCC. Content Type Journal ArticleCategory Original PaperPages 228-232DOI 10.1007/s00795-010-0496-1Authors Naoto Kuroda, Department of Diagnostic Pathology, Kochi Red Cross Hospital, 2-13-51 Shin-honmachi, Kochi, 780-8562 JapanMotoki Yamashita, Department of Urology, Kagawa University Faculty of Medicine, Kagawa, JapanYoshiyuki Kakehi, Department of Urology, Kagawa University Faculty of Medicine, Kagawa, JapanOndrej Hes, Department of Pathology, Charles University Hospital Plzen, Plzen, Czech RepublicMichal Michal, Department of Pathology, Charles University Hospital Plzen, Plzen, Czech RepublicGang-Hong Lee, Departments of Pathology, Kochi Medica... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520335 Fri, 16 Dec 2011 16:47:30 +0100 5520335 http://www.medworm.com/index.php?rid=5520334&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx070671678p244x5%2F We report a woman in her early thirties with a long-term history of systemic lupus erythematosus (SLE) and prednisolone administration, who progressed to Epstein-Barr virus (EBV)-positive lymphoproliferative disorder (LPD). Treatment for SLE consisted of 1 mg/kg/ day prednisolone followed by 5 mg/day of maintenance therapy. Lymph node biopsies were performed when the patient was in her early thirties, mid-forties, and late fifties. Histologically, the initial lymph node lesion was characterized by numerous enlarged, coalescing lymphoid follicles. The second biopsy showed effacement of the follicles and expansion of the paracortical area. A polymorphous population of small- to medium-sized lymphocytes, plasma cells, and immunoblasts had diffusely infiltrated the paracortical area. In ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520334 Fri, 16 Dec 2011 16:47:30 +0100 5520334 http://www.medworm.com/index.php?rid=5520337&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6q4186328t744731%2F Abstract  Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc 1638T/1638T mice, which express a truncated Apc that lacks the C-terminal domain. Apc 1638T/1638T mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc 1638T/1638T mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc 1638T/1638T and Apc +/+ mice. Thyroid follicle size was significantly larger in Apc 1638T/1638T mice than in Apc +/+ mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injectio... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520337 Fri, 16 Dec 2011 16:47:26 +0100 5520337 http://www.medworm.com/index.php?rid=5520338&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5040hn8q25w70287%2F Abstract  The prevalence of dopamine and cAMP-regulated phosphoprotein 32kD (DARPP-32) is associated with the pathogenesis of schizophrenia. To date, the findings on DARPP-32 cellular expression and distribution in postmortem brains from patients with schizophrenia have been inconsistent. To clarify the detailed cellular expression of DARPP-32 in patients with schizophrenia, we immunohistochemically stained sections from postmortem brains using specific antibodies. We measured the density of immunopositive cells in various brain regions including the prefrontal cortex and compared the data from nine schizophrenia subjects with those of nine age- and sex-matched control subjects. The density of DARPP-32-immunoreactive (IR) neurons was significantly lower in layers II-V of th... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520338 Fri, 16 Dec 2011 16:47:25 +0100 5520338 http://www.medworm.com/index.php?rid=5520340&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fcm4536536316263v%2F Abstract  Hepatocellular carcinoma (HCC) is the most common primary cancer worldwide. The only current drug available for clinical treatment of HCC is sorafenib, which inhibits multiple signaling kinases including Raf family members, platelet-derived growth factor receptor, vascular endothelial growth factor receptors 1 and 2, c-Kit, and Fms-like tyrosine kinase 3. Many studies have revealed that the mechanism underlying the antitumor effect of sorafenib is complex. Because sorafenib inhibits C-Raf more potently than B-Raf, the therapeutic efficacy of sorafenib is strongly influenced by the relative expression and activity of B-Raf and C-Raf and the complex interactions between these factors. Moreover, Rafindependent signaling mechanisms have recently emerged as important p... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520340 Fri, 16 Dec 2011 16:47:24 +0100 5520340 http://www.medworm.com/index.php?rid=5520339&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl738m76417238727%2F In this study, the persistently infected virus in the AGS cell line was determined to be infective and form reproducible virions, and a new morphological feature of SV5 was determined. Content Type Journal ArticleCategory Original PaperPages 213-220DOI 10.1007/s00795-010-0530-3Authors Yukimasa Ooi, Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686 JapanEriko Daikoku, Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686 JapanHong Wu, Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686 JapanHiroaki Aoki, Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daiga... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5520339 Fri, 16 Dec 2011 16:47:24 +0100 5520339 http://www.medworm.com/index.php?rid=5233418&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk3420156816p7734%2F In conclusion, pathologists should consider IgG4-related lymphadenopathy when diagnosing a lesion with IPT-like features. Content Type Journal ArticleCategory Original PaperPages 179-182DOI 10.1007/s00795-010-0525-0Authors Yasuharu Sato, Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558 JapanMasaru Kojima, Department of Anatomic and Diagnostic Pathology, Dokkyo University School of Medicine, Mibu, JapanKatsuyoshi Takata, Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558 JapanXingang Huang, Department of Pathology, Okayama University Graduate School of Medicine, Dentis... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233418 Thu, 15 Sep 2011 15:52:13 +0100 5233418 http://www.medworm.com/index.php?rid=5233417&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu51473107233614k%2F Abstract  We investigated the postnatal formation and origin of the microcyst, which are not fully elucidated at present, in the cochlear nucleus of gerbils. Sixty-six Mongolian gerbils were investigated at the light microscope level, and 35 of them were observed at the electron microscopic level. Foamy structures were evidently found at 2 days of age and remained unchanged through 4–8 days. The first small vacuole, presumably the former microcyst, appeared at 8 days. Myelin sheath bundles first appeared at 13 days. Electron-dense bodies were frequently found in the junction of the superficial layer and the deep layer at 2 days. The medium-sized vacuole was found in close association with the spherical bushy cells in the anteroventral cochlear nucleus (AVCN) as early as 5... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233417 Thu, 15 Sep 2011 15:52:13 +0100 5233417 http://www.medworm.com/index.php?rid=5233416&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F46488lp52v462184%2F Abstract  A 73-year-old woman showed marked exophytic growth of a tumor (25 × 23 × 14 mm) of the nipple over a period of 2 months. Histologically, numerous tumor nodules with no apparent keratinization were observed in the exophytic lesion. The tumor cells also showed little invasion to the dermis and no metastasis to the axillary lymph nodes (LN). The tumor cells were immunohistochemically positive for cytokeratins (CKs; AE1/AE3 and 34βE12), epithelial membrane antigen (EMA), and p53, but negative for Ber-EP4 and human papilloma virus (HPV). The MIB-1 index was 56%. Some tumor cells were also positive for some neuroendocrine markers, and showed some tonofilaments and neurosecretory granules in the cytoplasm under electron microscopy. We made the differential diagnosis o... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233416 Thu, 15 Sep 2011 15:52:13 +0100 5233416 http://www.medworm.com/index.php?rid=5233419&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft471824550529m53%2F Abstract  High-grade carcinoma with a large central acellular zone (central acellular carcinoma, CAC) and matrixproducing carcinoma (MPC) are aggressive tumors that both have a central myxomatous acellular zone. Their characteristic morphology may be useful in diagnostic imaging. Ultrasonographic findings based on the Breast Imaging Recording and Data System (BI-RADS) and detailed histological features were evaluated in 11 cases of CAC and 2 cases of MPC to characterize their features. Safranin-O staining was undertaken for the evaluation of central acellular zones in these tumors. Overall, ultrasonography demonstrated heterogeneous hyperechoic lesions in the center of the hypoechoic mass. Posterior echo enhancement was observed in all but 1 case. One case was classified as... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233419 Thu, 15 Sep 2011 15:52:12 +0100 5233419 http://www.medworm.com/index.php?rid=5233420&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjl93363215031174%2F Abstract  Lymph node metastasis of chromophobe renal cell carcinoma (RCC) is extremely rare. It has been recently reported that sarcomatoid chromophobe RCC frequently show polysomy of chromosomes 1, 2, 6, 10, and 17. In this article, we report an unusual case of chromophobe RCC. A 42-year-old Japanese woman presented with hematuria and complained of inguinal pain 2 months after the initial symptoms. Radical nephrectomy and renal hilar lymphadenectomy were performed. The tumor was 8 cm in greatest diameter; its cut surface was beige in color. Large metastasis to the renal hilar lymph node was identified. Histological examination of the right renal tumor met the criteria of chromophobe RCC. In addition to histological findings of typical chromophobe RCC, small cell foci, come... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233420 Thu, 15 Sep 2011 15:52:11 +0100 5233420 http://www.medworm.com/index.php?rid=5233422&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh133185525n96r05%2F Abstract  We studied the effects of short-term estrogen treatment (STET) on the progression of mammary lesions from ductal hyperplasia (DH) through ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) in the N-methyl-N-nitrosourea (MNU)-induced rat mammary carcinogenesis model. Three-week-old female Lewis rats (n = 40) received an intraperitoneal injection of MNU (50 mg/kg). Three weeks later, a 3-week-release, 0.25-mg, 17β-estradiol pellet was subcutaneously implanted for 2 weeks in 20 rats (STET); the remaining 20 rats did not receive the estradiol pellets (age-matched control). All rats were killed at 12 weeks of age, and their abdominal-inguinal mammary glands were histologically examined. The incidence and multiplicity of DHs were similar between groups (S... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233422 Thu, 15 Sep 2011 15:52:10 +0100 5233422 http://www.medworm.com/index.php?rid=5233421&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr3183207753x5630%2F Abstract  Stroke-prone spontaneously hypertensive rats (SHRSP) are known to show necrosis of the femoral head with a frequency of about 50%. This rat has thus been used as an animal model for necrosis of the femoral head in many studies. In a detailed investigation of feeding vessel disorders that cause femoral head necrosis, we observed changes over time in the feeding vessels using scanning electron microscopy and transmission electron microscopy. In scanning electron microscopy of vascular casts, abnormal findings in feeding vessels of SHRSP with aging from the immature stage included contortion and bending in the lumen with overall narrowing. Under transmission electron microscopy, decreased numbers of smooth muscle cells and increased amounts of collagen fibers were ma... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233421 Thu, 15 Sep 2011 15:52:10 +0100 5233421 http://www.medworm.com/index.php?rid=5233423&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1166418636622535%2F We describe here the development of cell biology technique and the experimental study of regeneration in SCI. The aim of this study was to investigate the potential for neurological recovery by transplantation neurospheres (NS) derived from BMSCs into thoracic SCI. Adult Fisher rats were used: 45 were subjected to complete thoracic SCI performed by the balloon compression method. BMSCs were cultured in vitro to obtain NS. Seven days after thoracic SCI, groups of 15 rats each received transplants of BMSCs-NS (group A), BMSCs (group B), or injection of medium only (group C) into the SCI lesion. Rats from each group were evaluated and compared longitudinally for motor function recovery. The spinal cords (SC) of injured rats were harvested at day 21 or day 42 and prepared for histologica... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233423 Thu, 15 Sep 2011 15:52:09 +0100 5233423 http://www.medworm.com/index.php?rid=5233425&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp217850924221508%2F In conclusion, FAS expression can be a biomarker for tumor aggressiveness and loss of differentiation of bladder cancer, and the evaluation of its expression level in combination with Ki-67 labeling index may be a precise predictor for poor prognosis of cancer patients. Content Type Journal ArticleCategory Original PaperPages 146-150DOI 10.1007/s00795-010-0517-0Authors Takashi Sugino, Department of Basic Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295 JapanKeiichi Baba, Department of Urology, Fukushima Medical University School of Medicine, Fukushima, JapanNobuo Hoshi, Department of Basic Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295 JapanKen Aikawa, Department of Urology, Fukushima M... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233425 Thu, 15 Sep 2011 15:52:08 +0100 5233425 http://www.medworm.com/index.php?rid=5233424&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy4t077306728h1h6%2F Abstract  Hepatocellular carcinoma (HCC) has an aggressive clinical course with frequent recurrence and metastasis. Orthotopic liver transplantation has been the only curative tool for unresectable HCC; therefore, recent advances in molecular targeted therapy may improve the prognosis of HCC. The multiple kinase inhibitor sorafenib and the macrolide antibiotic rapamycin are currently the most promising agents for treating unresectable HCC. A large population-based clinical trial revealed that sorafenib significantly prolonged the overall survival of HCC patients. However, subsequent clinical studies showed that sorafenib rarely reduced tumor volume and inadequately prolonged survival of patients with severe liver damage. To improve its therapeutic effect, the development of... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=5233424 Thu, 15 Sep 2011 15:52:08 +0100 5233424 http://www.medworm.com/index.php?rid=4997143&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm463783478510130%2F Abstract  S100A1 is a calcium-binding protein and a member of the S100 family. Recently, S100A1 immunohistochemistry may be an available marker in the differential diagnosis between renal oncocytoma and chromophobe renal cell carcinoma (RCC). However, there are no reports on S100A1 expression in oncocytic papillary RCC that has been recently identified. In this article, we immunohistochemically examined the expression of S100A1 protein in 18 renal tumors including 4 renal oncocytoma, 10 chromophobe RCCs, and 4 oncocytic papillary RCCs. All the cases of renal oncocytoma and oncocytic papillary RCC showed a positive reaction for S100A1 with cytoplasmic pattern. In chromophobe RCC, 3 of 4 tumors with typical variant and 4 of 6 tumors in eosinophilic variant were completely neg... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997143 Thu, 30 Jun 2011 05:51:44 +0100 4997143 http://www.medworm.com/index.php?rid=4997144&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F50p063rlx02177x1%2F Abstract  Tumor behavior depends on the complex tumor interstitium and microenvironment, which influence transport of fluid and soluble molecules from blood vessels. The purpose of this study was to reveal how complex tumor tissues affect the immunodistribution of serum proteins and time-dependent translocation of bovine serum albumin (BSA) from blood vessels, using relatively differentiated human adenocarcinoma produced by the xenografted A549 cell line. Histological architecture and immunodistribution of the serum proteins in adenocarcinomatous tissues were clearly detected by the in vivo cryotechnique and cryobiopsy. Both albumin and IgG1 were detected in blood vessels, connective tissues around the tumor mass, and the interstitium among tumor cell nests. IgM was mainly ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997144 Thu, 30 Jun 2011 05:51:43 +0100 4997144 http://www.medworm.com/index.php?rid=4997146&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F42173076mr31u5l3%2F Abstract  Improvement of diagnostic accuracy for pancreatic cancer in pancreatic disease patients was investigated by examining the combination of three diagnostic methods, i.e., measurements of RCAS1 and CEA levels in pancreatic juice and pancreatic juice cytology. Pancreatic juice was collected from 12 pancreatic cancer (PC) and 26 non-PC patients. RCAS1 and CEA levels were measured by using ELISA. RCAS1 expression on surgically resected tissue was immunohistochemically examined for 2 PC patients. By setting the cutoff level of RCAS1 at 10 U/ml and that of CEA at 18.5 μg/ml, sensitivity of RCAS1 was 42% and that of CEA was 50%. On the other hand, sensitivity and specificity increased from 42% and 85% of RCAS1 alone to 75% and 85% in the examination of RCAS1 + CEA + cytol... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997146 Thu, 30 Jun 2011 05:51:40 +0100 4997146 http://www.medworm.com/index.php?rid=4997145&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc628710354q36264%2F Abstract  Ameloblastoma (AB), which is the most common odontogenic tumor, may originate from the dental lamina remnants. The expression of CD56, which is a transmembrane molecule, is associated with neuroectodermal differentiation of the embryonal cells. The aim of this study was to evaluate the expression of CD56 in AB, in comparison with other odontogenic cysts. We used formalin-fi xed, paraffi n-embedded specimens from 34 cases of AB, 10 cases of keratocystic odontogenic tumor (KCOT), and 7 cases of dentigerous cyst (DC). We immunohistochemically examined CD56, NeuroD1, and N-cadherin expression in these tumors as compared with the expression patterns of various epithelial markers. Seventy-four percent of AB showed immunopositivity for CD56, and both CD56 and N-cadherin ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997145 Thu, 30 Jun 2011 05:51:40 +0100 4997145 http://www.medworm.com/index.php?rid=4997147&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk505308w8418m1nl%2F Abstract  Both liver epithelial and oval cells are believed to be liver stem cells. We investigated the identification by producing monoclonal antibodies against liver epithelial cells. Monoclonal antibodies against hepatic stem-like cells (HSL cells) have been selected to follow the hepatic stem cells during hepatic regeneration and developmental changes in the liver. Monoclonal antibodies were induced by immunization of BALB/c mice with HSL cells established from the epithelial cells of the adult rat liver. The hybridomas were screened by indirect immunofluorescence staining of HSL cells. We produced a unique monoclonal antibody against HSL cells, MabH, which specifically recognizes liver epithelial cells. MabH did not react with liver parenchymal cells but did react with... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997147 Thu, 30 Jun 2011 05:51:39 +0100 4997147 http://www.medworm.com/index.php?rid=4997149&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2641664630564265%2F Abstract  We investigated the origin of the apical transcytic membrane system in jejunal absorptive cells of neonatal rats using light, electron, and immunofluorescence microscopy. In rats just after birth, intraluminally injected horseradish peroxidase (HRP), used as a macromolecular tracer, was observed only in the apical endocytic membrane system including the lysosomes, of jejunal absorptive cells in vivo. No tracer, however, was found in the intercellular space between the jejunal absorptive cells and the submucosa. Immunoreactive neonatal Fc receptor (FcRn) was localized in the perinuclear region of these absorptive cells whereas immunoglobulin G (IgG) was not found in these absorptive cells. In contrast, in rats 2 h after breast-feeding, intraluminally injected HRP w... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997149 Thu, 30 Jun 2011 05:51:37 +0100 4997149 http://www.medworm.com/index.php?rid=4997148&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ftr335153767n3747%2F Abstract  Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH & IHC) has sufficient ultrastructural resolution to provide two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots; Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH & IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationship between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin revealed that both rab3B and SNARE syste... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4997148 Thu, 30 Jun 2011 05:51:37 +0100 4997148 http://www.medworm.com/index.php?rid=4630823&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F444372732556qxh6%2F Abstract  To further characterize the morphology and dynamics of focal contacts (FCs) in epithelial cells, we compared the size, number, localization, velocity, and turnover of FCs in epithelial and mesenchymal cell lines. Using immunocytochemistry, we found there were no significant differences between mesenchymal and epithelial cells in number and appearance whereas the location and size of FCs in each cell were different between mesenchymal and epithelial cells. FCs in mesenchymal cells localized at the cell periphery and cell center, but FCs were found only at the cell periphery in epithelial cells. The size of FCs in epithelial cells were significantly smaller than in mesenchymal cells. Next, we compared the dynamics of FCs in both mesenchymal and epithelial cells and ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630823 Tue, 22 Mar 2011 05:58:48 +0100 4630823 http://www.medworm.com/index.php?rid=4630822&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl7g7023h8570132n%2F Abstract  A 66-year-old male patient underwent a stomach-preserving pancreatoduodenectomy procedure because of a tumor located around the lower bile duct under the diagnosis of carcinoma of the lower bile duct. The tumor (3.5 × 2.5 cm) was found at the head of the pancreas and had invaded the papillae of Vater at the duodenum. Histology findings indicated both ductal adenocarcinoma and endocrine tumor. The ductal adenocarcinoma component expressed carcinoembryonic antigen, cytokeratin (CK)-19, CK-20, carbohydrate 19-9, and amylase, whereas the endocrine component, which occupied about one-third of the tumor, expressed glucagon, neuron-specific enolase, and chromogranin A. The Ki-67 labeling indices of the two components were 49.7% and 5.3%, respectively. Herein, we present... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630822 Tue, 22 Mar 2011 05:58:48 +0100 4630822 http://www.medworm.com/index.php?rid=4630825&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3845173j22844112%2F Abstract  Previous studies have demonstrated that the blood-testis barrier (BTB) at the tubuli recti (TR) and the rete testis (RT) is less complete than at the seminiferous tubules (ST). However, there has been no report focusing on the basal lamina, which is an important component of the BTB at both TR and RT. In the present study, we performed electron microscopic observation of the basal lamina at the TR and RT, in comparison with that of those of the ST in normal mice. The results showed that the basal lamina of modified Sertoli cells at the TR segment exhibited a wavy and multilayered structure, but the Sertoli cells of ST and the epithelium of RT had an almost flat and singlelayered basal lamina. It was also noted that wide gaps existed between the modified Sertoli ce... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630825 Tue, 22 Mar 2011 05:58:46 +0100 4630825 http://www.medworm.com/index.php?rid=4630824&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3063534p558n3rlh%2F In this study, the relationship between the cytoarchitecture of the LMM or the distribution of lymphatic vessels and cancer invasion is discussed. Content Type Journal ArticlePages 39-45DOI 10.1007/s00795-010-0503-6Authors Yuichi Akashi, Department of Oncological Science (Surgery II), Faculty of Medicine, Oita University, Idaigaoka 1-1, Yufu, Oita, 879-5593 JapanTsuyoshi Noguchi, Department of Gastroenterological Surgery, Faculty of Medicine, Oita University, Oita, JapanKaoruko Nagai, Department of Oncological Science (Surgery II), Faculty of Medicine, Oita University, Idaigaoka 1-1, Yufu, Oita, 879-5593 JapanKatsunobu Kawahara, Department of Oncological Science (Surgery II), Faculty of Medicine, Oita University, Idaigaoka 1-1, Yufu, Oita, 879-5593 JapanTatsuo Shimada, Department o... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630824 Tue, 22 Mar 2011 05:58:46 +0100 4630824 http://www.medworm.com/index.php?rid=4630826&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj37141623q3w6064%2F Abstract  The function of brush cells is obscure, but recent cytochemical studies indicate that rat bile duct brush cells secrete NaHCO3. The aim of this study was to determine the quantitative distribution of brush cells at 16 sites of the rat gastrointestinal tract and to investigate the role of NaHCO3 secretion at these sites. Specimens of 16 sites of the gastrointestinal tracts of three female Long-Evans rats were fixed in a periodate-lysine-paraformaldehyde solution. Frozen sections were stained with the anti-cytokeratin 18 antibody, a selective marker for brush cells. The numbers of brush cells were counted from photographs. The percentages of brush cells in the epithelium at the 16 sites were gastric groove, 32.3%; corpus adjacent to the gastric groove, 2.5%; corpus, 0... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630826 Tue, 22 Mar 2011 05:58:45 +0100 4630826 http://www.medworm.com/index.php?rid=4630828&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq012571553715600%2F Abstract  Translocation-type renal carcinoma has been recently discovered, and it is possible that this tumor may have been previously diagnosed as other types of renal tumor. We have subjected 41 renal tumors, including VHL gene mutation-negative clear cell renal cell carcinoma (RCC), papillary RCC, and chromophobe RCC, to immunohistochemistry of transcription factor E3 (TFE3) and TFEB. All tumors were histologically evaluated by additional immunohistochemical study. As a result, 5 tumors showed a positive reaction for TFE3 with a range from 1+ to 2+ in intensity. No tumors were positive for TFEB. In 5 tumors immunohistochemically positive for TFE3, chimeric transcripts including ASPL-TFE3, PRCC-TFE3, CLTCTFE3, PSF-TFE3, or Nono-TFE3 were not detected. The diagnosis of 6 tum... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630828 Tue, 22 Mar 2011 05:58:41 +0100 4630828 http://www.medworm.com/index.php?rid=4630827&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F172n55t3qj842515%2F We report a case of Chlamydophila (C.) pneumoniae infection presenting with fever and rapid intrahepatic cholestasis. A 63-year-old man had a week-long history of intermittent high fever and rapidly progressive jaundice with atypical erythema. The results of liver function tests were recorded. The results of all serological tests were negative; the IgM, IgG, and IgA titers for C. pneumoniae had increased, which indicates a C. pneumoniae infection. The patient’s fever and liver dysfunction improved upon administration of minocycline. Light microscopic findings showed the presence of enlarged liver cells with clear cytoplasm, a few mitotic figures, multinucleated cells, and bile cholestasis. The electron microscopic appearance of liver biopsy showed that bile canaliculi exhibited intra... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630827 Tue, 22 Mar 2011 05:58:41 +0100 4630827 http://www.medworm.com/index.php?rid=4630830&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy2x7px23h6m21964%2F Abstract  Plectin is a versatile cytoplasmic cross-linking protein that connects intermediate filaments to microfilaments, microtubules, and membrane adhesion sites. The cross-linking functions of plectin help organize the cytoskeleton into a stable meshwork important for maintaining uniformity in cell size and shape. As cells of hepatocellular carcinoma are morphologically different from normal human hepatocytes, we hypothesized that altered plectin expression and cytoskeletal organization underlies this pleomorphic transformation. To test this hypothesis, we analyzed expression levels and organization of all cytoskeletal elements, including intermediate filaments, microfilaments, and microtubules, after plectin knockdown in human Chang liver cells. We found that expressio... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630830 Tue, 22 Mar 2011 05:58:40 +0100 4630830 http://www.medworm.com/index.php?rid=4630829&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8g581700h262r8l1%2F This study evaluated the expression of biological markers of breast cancers with brain metastases. Eighteen paired tumors were assessed, with 42 non-brain-metastasizing breast cancers that were stained with ER, PR, HER2, CK5/6, p63, and Ki67, and were also classified into intrinsic subtypes. The expression patterns between the breast tumors with brain metastases were compared to the brain metastases and the controls. Breast cancers with brain metastases were of higher grade and showed higher incidence of lymph node metastases at initial diagnosis and higher EGFR, p63, and Ki67 expression. In the group of breast cancers with brain metastases, the brain metastases showed higher HER2, CK5/6, and Ki67 expression compared to the breast primaries. There was also a higher incidence of basal... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630829 Tue, 22 Mar 2011 05:58:40 +0100 4630829 http://www.medworm.com/index.php?rid=4630831&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F075207l818618206%2F Abstract  Desquamation in the mammalian skin is a well-balanced process of producing corneocytes and shedding them from the surface of the skin. The corneodesmosome, which is a modified desmosome, is the main adhesive structure in the cornified cell layer. The major extracellular constituents of corneodesmosomes are desmoglein 1, desmocollin 1, and corneodesmosin. Proteases involved in the degradation of corneodesmosomes and their inhibitors are secreted from lamellar granules in the granular cell layer. Genetic defects in corneodesmosin and protease inhibitors result in accelerated desquamation and severe barrier impairment. Abnormalities in transportation and secretion of lamellar granules underlie ichthyosis seen in certain human diseases. Content Type Journal Articl... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4630831 Tue, 22 Mar 2011 05:58:39 +0100 4630831 http://www.medworm.com/index.php?rid=4411080&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F176q408833826251%2F We present herein a case of primary splenic MCCa resulting in pseudomyxoma peritonei (PMP). A 66-year-old Japanese woman presented to a hospital with a chief complaint of upper abdominal pain and a 7-year history of splenic cyst. Cyst rupture was noted on computed tomography, and splenectomy was performed. The abdominal cavity was filled with a large amount of gelatinous ascites, with the appearance of PMP. On the cut surface, multiple cysts containing mucinous material were found within and outside the spleen. Microscopically, splenic parenchyma was occupied by large mucinous pools focally lined with mucinous epithelial cells and mesothelial cell-like cells, which were considered benign. Outside the spleen, a low-grade MCCa component was found. No ectopic pancreatic or intestinal ti... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411080 Wed, 26 Jan 2011 02:57:58 +0100 4411080 http://www.medworm.com/index.php?rid=4411079&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9912h58007442451%2F Abstract  Hybrid sclerosing adenosis and basal cell hyperplasia of the prostate is a rare lesion. Here we report the seventh case of such lesions. Histological examination of the transurethral resection of the prostate of a 83-year-old Japanese man showed a small lesion consisted of sclerosing adenosis and basal cell hyperplasia, in addition to the diffuse glandular and fibromuscular hyperplasia. Immunohistochemically, many basal cells in sclerosing adenosis and basal cell hyperplasia areas showed a positive reaction for p63, cytokeratin 5, and D2-40. Additionally, many basal cells in the sclerosing adenosis area and some basal cells in the basal cell hyperplasia area were positive for S-100 protein and alpha-smooth muscle actin, which are myoepithelial cell markers. Finall... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411079 Wed, 26 Jan 2011 02:57:58 +0100 4411079 http://www.medworm.com/index.php?rid=4411081&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg2r152h454074035%2F Abstract  Two cases of skull base chordoma (case 1, a 57-yearold woman; case 2, a 69-year-old woman) were investigated immunohistochemically and ultrastructurally. The tumors showed histopathological features typical of chondroid chordoma and contained both classical chordomatous and hyaline cartilaginous components. Tumor cells were immunoreactive for cytokeratin, vimentin, and S-100 protein, but negative for microtubule-associated protein 2 and class III beta-tubulin (tub-B3). Tumor cells of case 2 were immunoreactive for tauprotein and class II beta-tubulin (tub-B2), whereas those of case 1 were negative. Ultrastructurally, tumor cells in both cases showed the presence of abundant glycogen granules, well-developed intracellular organelles, and desmosome-like junctions. I... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411081 Wed, 26 Jan 2011 02:57:57 +0100 4411081 http://www.medworm.com/index.php?rid=4411083&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff0231k17k808260k%2F In conclusion, PDAC cells showed morphological changes, spheroid formation, and alterations of cytoskeletal proteins in 3D culture. E-cadherin might be one of the key molecules involved in spheroid formation of PDAC cells. The 3D spheroidal culture system was a useful method for cell imaging with contrast-phase microscopy and confocal microscopy. Content Type Journal ArticlePages 211-217DOI 10.1007/s00795-010-0497-0Authors Yoko Matsuda, Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602 JapanToshiyuki Ishiwata, Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602 JapanYoko Kawamoto, Department of Pathology, Integrative Oncological Pathol... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411083 Wed, 26 Jan 2011 02:57:55 +0100 4411083 http://www.medworm.com/index.php?rid=4411082&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp56k3325kl8nk4u3%2F Abstract  The most critical step for initiation and progression of estrogen receptor-α (ERα)-positive breast cancers is thought to be upregulation of ERα expression. There are several factors involved in this mechanism, i.e., increased promoter activity of the ERα gene (ESR1) at the transcriptional level, ESR1 gene amplification, and diminished degradation of ERα protein through ubiquitination and proteasomal pathways. Mediating these factors, ERα protein levels seem to be controlled, although the details of the mechanism remain to be clarified. In addition, for upregulation of estrogen signaling, functional changes in its action in cancer cells originating from normal epithelial cells, i.e., estrogen stimulation, which then leads to proliferation of ERα-positive canc... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411082 Wed, 26 Jan 2011 02:57:55 +0100 4411082 http://www.medworm.com/index.php?rid=4411086&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F635712m16r34h118%2F We report a case of primary sebaceous carcinoma of the tongue. Systemic imaging studies revealed that the patient had a T2N2cM0 (International Union Against Cancer guidelines) primary lingual tumor. Histopathological examination revealed neoplastic sebocytic and basaloid cells, and Sudan III staining and electron microscopy revealed intracytoplasmic lipid droplets. The neoplastic cells stained positive for adipophilin; epithelial membrane antigen; epithelial antigen; and cytokeratins 7, 8, and 15, but negative for cytokeratins 5/6, 18, 19, and 20; the androgen receptor; and carcinoembryonic antigen. Superselective intraarterial chemotherapy was administered via the superficial temporal artery concurrent with daily radiotherapy. Multiple biopsies confirmed a complete response of the p... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411086 Wed, 26 Jan 2011 02:57:54 +0100 4411086 http://www.medworm.com/index.php?rid=4411085&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Feq602280w040k870%2F In conclusion, silver-enhanced immunogold scanning electron microscopy at low acceleration voltages using vibratome sections can be applicable for detecting the intracellular/extracellular localization of molecules in solid tissues. We succeeded in visualizing the enhanced albumin endocytosis of the proximal tubules and the exocytosis of albumin from podocytes in the nephrotic rats. Content Type Journal ArticlePages 218-225DOI 10.1007/s00795-010-0500-9Authors Satoshi Kinugasa, Division of Nephrology and Endocrinology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655 JapanAkihiro Tojo, Division of Nephrology and Endocrinology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655 JapanTatsuo Sakai, Department of Anatomy, Juntendo University School of Medicine, T... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411085 Wed, 26 Jan 2011 02:57:54 +0100 4411085 http://www.medworm.com/index.php?rid=4411084&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx65580302325hl57%2F Abstract  Epidemiological studies have indicated a relationship between gonadal steroid hormones, primarily estrogens, and epithelial ovarian carcinoma. In situ estrogen metabolism and synthesis have been considered to play important roles in the development of the progression of epithelial ovarian carcinoma. 17β-Hydroxysteroid dehydrogenases (17β-HSDs) are a group of intracellular isozymes catalyzing interconversions between estradiol (E2) and estrone (E1). In the last step of steroidogenesis, 17β-HSD type 1 catalyzes the 17β-reduction and produces E2 from E1. The oxidative enzymes known as types 2, 4, and 8 are potent estrogen-inactivating enzymes that convert E2 to E1. Here we report the immunoexpression of 17β-HSD types 1, 2, 4, and 8 in normal human ovarian surfac... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411084 Wed, 26 Jan 2011 02:57:54 +0100 4411084 http://www.medworm.com/index.php?rid=4411087&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd5n222h19604770r%2F Abstract  Human beta-defensins (hBDs), a group of antimicrobial peptides, are involved in the protective barrier of the oral epithelium. Nicotine induces periodontal and oral epithelial diseases. The purpose of the present study was to investigate the effect of nicotine on the expression pattern of hBD-2 in keratinocytes. HaCaT cells, a keratinocyte cell line, were incubated with 8, 15, 30, or 80 μM nicotine for 24 h. Expression of hBD-2 was observed by RT-PCR, qRTPCR, and ELISA assay. The cells were treated with inhibitors for intracellular pathways (p38MAP kinase, NF-κB, JNK, MAPK-ERK) and with nicotinic acetylcholine receptor (nAChR) inhibitors in a series of experiments. Data were analyzed using Student’s t test. qRT-PCR revealed that the expression level of hBD-2 mR... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411087 Wed, 26 Jan 2011 02:57:52 +0100 4411087 http://www.medworm.com/index.php?rid=4411089&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl872606833137nhj%2F Content Type Journal ArticlePages 254-254DOI 10.1007/s00795-010-0527-yAuthors Naoto Kuroda, Department of Diagnostic Pathology, Kochi Red Cross Hospital, 2-13-51 Shin-honmachi, Kochi, 780-8562 JapanDmitry V. Kazakov, Department of Pathology, Charles University Hospital, Pilsen, Czech RepublicMichal Michal, Department of Pathology, Charles University Hospital, Pilsen, Czech Republic Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 43 Journal Issue Volume 43, Number 4 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411089 Wed, 26 Jan 2011 02:57:51 +0100 4411089 http://www.medworm.com/index.php?rid=4411088&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl65wm788933q1911%2F Content Type Journal ArticlePages 253-253DOI 10.1007/s00795-010-0526-zAuthors Teresa Pusiol, Institute of Anatomic Pathology, Rovereto Hospital, Rovereto, ItalyMaria Grazia Zorzi, Institute of Anatomic Pathology, Rovereto Hospital, Rovereto, ItalyDoriana Morichetti, Institute of Anatomic Pathology, Rovereto Hospital, Rovereto, ItalyFrancesco Piscioli, Institute of Anatomic Pathology, Rovereto Hospital, Rovereto, Italy Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 43 Journal Issue Volume 43, Number 4 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411088 Wed, 26 Jan 2011 02:57:51 +0100 4411088 http://www.medworm.com/index.php?rid=4411090&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4215l786hw127801%2F Abstract  Acquired cystic disease (ACD)-associated renal cell carcinoma (RCC) has been recently described. To date, there are no reports on genetic findings of G-band karyotype of ACD-associated RCC. In this article, we report the first report of G-band karyotype of ACD-associated RCC. A 66-year-old Japanese man was found to have a left renal tumor during the follow-up of hemodialysis consequent to chronic renal failure. Left nephrectomy was performed. Histological examination of three tumors in the left kidney showed the cribriform or microcystic growth pattern of neoplastic cells with eosinophilic cytoplasm, and many oxalate crystals were observed. The G-band karyotype of ACD-associated RCC showed 49, X, +X, −Y, +3, +7, +16. These chromosomal abnormalities resemble thos... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=4411090 Wed, 26 Jan 2011 02:57:38 +0100 4411090 http://www.medworm.com/index.php?rid=3997968&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F690525560q17813n%2F Abstract  In the human placenta, embryo-derived trophoblasts aggressively invade maternal spiral arteries and transform the arteries to low-resistance large-caliber vessels. This process, which ensures adequate placental perfusion, is called maternal vascular remodeling. Histological examination showed deposition of maternal platelets in the trophoblast aggregates formed in the spiral arteries. Several lines of evidence suggest that these platelets are activated. Soluble factors released from the activated platelets, as a whole, enhanced invasive capacity of isolated trophoblasts in vitro. These findings suggest the importance of nonhemostatic platelet function in maternal vascular remodeling. In contrast, gene knockout studies suggest that maternal platelet defects are com... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997968 Tue, 21 Sep 2010 03:42:46 +0100 3997968 http://www.medworm.com/index.php?rid=3997969&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmx48m52388k26455%2F We described the possibility of retinal regeneration using a novel and efficient technique for culturing and separating retinal stem/progenitor cells from iris tissue. Immunohistochemical staining of adult agouti mouse iris tissue revealed the presence of nestin/low-affinity neurotrophin receptor p75 (p75NTR)-positive cells on the endothelium camerae anterioris side. Cultured mouse iris-derived cells contained little or no melanin and were found to be positive for nestin. Most nestin-positive cells were analyzed for the coexpression of p75NTR as a cell membrane protein. When the p75NTR was used as a marker to sort the cells, we obtained a dense population of nestin-positive cells. Furthermore, the nestin/p75NTR-positive cells were able to differentiate into neural retina cells. Thus, th... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997969 Tue, 21 Sep 2010 03:42:45 +0100 3997969 http://www.medworm.com/index.php?rid=3997970&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk104044lv4276869%2F Abstract  Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were p... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997970 Tue, 21 Sep 2010 03:42:26 +0100 3997970 http://www.medworm.com/index.php?rid=3997971&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbl32282r65855528%2F In conclusion, the combination of immunohistochemistry of P504S, cytokeratin 5, p63, and D2-40 may contribute to the accurate diagnosis of ASAP in the initial prostatic needle biopsy. Content Type Journal ArticleDOI 10.1007/s00795-008-0435-6Authors Naoto Kuroda, Department of Diagnostic Pathology, Kochi Red Cross Hospital, 2-13-51 Shin-honmachi, Kochi, 780-8562 JapanKazunobu Katto, Department of Urology, Kochi Red Cross Hospital, Kochi, JapanMasato Tamura, Department of Urology, Kochi Red Cross Hospital, Kochi, JapanTomoyuki Shiotsu, Department of Urology, Kochi Red Cross Hospital, Kochi, JapanShoichiro Nakamura, Department of Urology, Kochi Red Cross Hospital, Kochi, JapanYuji Ohtsuki, Department of Pathology, Matsuyama-shimin Hospital, Ehima, JapanOndrej Hes, Department of Pathol... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997971 Tue, 21 Sep 2010 03:42:23 +0100 3997971 http://www.medworm.com/index.php?rid=3997972&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft6m767704t6260r0%2F Abstract  To examine the mRNA expression of hepatobiliary transporters in primary biliary cirrhosis (PBC) patients and to compare bile acid absorption, synthesis, and efflux in patients with non-end-stage and end-stage PBC, we obtained liver samples from PBC patients by percutaneous needle biopsy. End-stage PBC was defined as follows: histological stage IV; cirrhosis; serum total bilirubin, ≥4.0 mg/dl; and Child-Pugh Class C. The mRNA expression levels of sodium taurocholate cotransporting polypeptide (NTCP), bile salt export pump (BSEP), and hepatic cholesterol 7α-hydroxylase (CYP7A1) were significantly higher in the PBC patients than in the controls (P < 0.01). The mRNA levels of NTCP and BSEP were significantly higher in the end-stage PBC patients than in the contro... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997972 Tue, 21 Sep 2010 03:42:07 +0100 3997972 http://www.medworm.com/index.php?rid=3997973&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj830737842pj8158%2F This report presents an additional case of LG-SDC of the parotid gland. The patient was a 38-year-old Japanese woman who noticed painless swelling of the left parotid region. Grossly, the cut surface of the tumor was cystic. Microscopically, the tumor showed a multicystic pattern, which was lined by eosinophilic to clear atypical cells with cribriform or Roman bridge patterns. An immunohistochemical examination revealed the tumor was positive for cytokeratin (CK) 7 and epithelial membrane antigen, partially positive for androgen receptor and gross cystic disease fluid protein-15, and diffusely positive for Her-2/Neu, progesterone, and estrogen receptors. The cancer cells showed focal immunopositivity for S-100 protein. Immunostaining for p63, CK14, and calponin showed an in situ patt... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997973 Tue, 21 Sep 2010 03:41:21 +0100 3997973 http://www.medworm.com/index.php?rid=3997974&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa2661604l7777351%2F We report such a case of inflammatory myofibroblastic tumor of the mandible in a 14-year-old girl. The patient presented with an aggressive ulcerative soft tissue mass of 3 months duration in the mandibular molar gingiva. Histologically, the lesion was composed of fibroblastic or myofibroblastic spindle cell proliferations with infiltrative margins in an inflammatory background. Immunohistochemically, the fibroblastic or myofibroblastic spindle cells were positive for vimentin, α-smooth muscle actin, and Ki-67 (MIB-1) but negative for desmin, pan-cytokeratin, S-100 protein, CD34, CD68, CD99, bcl-2, β-catenin, estrogen receptor, progesterone receptor, ALK-1, and p53. These spindle cells were focally and weakly Ki-67- (MIB-1-) positive. The MIB-1 labeling index was 5%. The results o... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997974 Tue, 21 Sep 2010 03:41:09 +0100 3997974 http://www.medworm.com/index.php?rid=3997975&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2273v210h7212w97%2F Abstract  We recently reported the chromosome region maintenance 1 (CRM1)-dependent nuclear export of intron-less human interferon-α1 (IFN-α1) mRNA, which encodes a main effecter of host innate immunity. We show that the coding region of IFN-α1 mRNA forms novel secondary structures that are responsible for the CRM1-dependent export of the transcript. Deletion-mutagenesis, in vivo export assays, and computer analyses of the folding potentials of export-competent fragments revealed the presence of a domain, termed the conserved secondary structure (CSS), comprising two adjacent putative stable stem-loop structures (nt 208–452). Internal deletion-mutagenesis and constitutive export assays of each stem-loop structure demonstrated that subregions 308–322 and 352–434 act a... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997975 Tue, 21 Sep 2010 03:41:02 +0100 3997975 http://www.medworm.com/index.php?rid=3997976&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Frp78050705k1069n%2F We report herein the first case of such a tumor. A 67-year-old Japanese man presented with dull epigastralgia. The gastric endoscopy revealed an ulcerative cancerous lesion of the upper portion. Histological examination of the gastric tumor showed the coexistence of lymphoepithelioma-like carcinoma and adenosquamous carcinoma. The former showed a positive reaction for Epstein-Barr virus-encoded RNA (EBER). Neoplastic cells of the latter were immunoreactive for p63 and keratin 903, and Alcian blue stain detected acid mucin in glandular lumens. This pattern resembled mucoepidermoid carcinoma in the salivary gland. Finally, this is the first case of gastric lymphoepithelioma-like carcinoma associated with adenosquamous carcinoma, and clinicians and pathologists should recognize the pre... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997976 Tue, 21 Sep 2010 03:40:50 +0100 3997976 http://www.medworm.com/index.php?rid=3997977&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh55244rk88127217%2F Abstract  This is a case report of a 66-year-old woman who consulted us with a 1-week history of postprandial epigastric discomfort and dyspepsia. Upper and lower gastrointestinal endoscopy and double-balloon enteroscopy revealed lesions in three parts: a swelling with a shallow depression in the ampulla of Vater, flat and rough nodules in the jejunum, and a mixture of lymphoid polyposis and rough surface of follicular lymphoma of the terminal ileum and Bauhin valve. The histological, immunophenotypic, and molecular findings of the duodenal lesion confirmed the diagnosis of follicular lymphoma. We initially diagnosed the ileal lesion as MALT lymphoma immunohistochemically. However, Southern blot hybridization analysis for immunoglobulin heavy chain gene rearrangement showed... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3997977 Tue, 21 Sep 2010 03:40:32 +0100 3997977 http://www.medworm.com/index.php?rid=3822399&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn27876g7655r7465%2F We examined the effects of low protein intake on the development of the remaining kidney in subtotally (5/6) nephrectomized immature rats. Three-week-old rats were kept on a diet containing either 12% protein (Lp rats) or 18% protein (Np rats) for 4 or 8 weeks after subtotal nephrectomy (SUNx). In Western blot analysis, the endothelial NO synthase (eNOS) protein expression of the Lp rats was significantly higher than that of the Np rats at 4 weeks after SUNx. Immunohistochemically, more inducible NO synthase (iNOS)-positive cells were observed in the Np rats than in the Lp rats 4 weeks after SUNx in the distal tubules. In semiquantitative RT-PCR, the expression of renin mRNA was significantly lower in the Lp rats than in the Np rats at 4 and 8 weeks after SUNx. These findings reveal ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822399 Tue, 03 Aug 2010 17:01:12 +0100 3822399 http://www.medworm.com/index.php?rid=3822401&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm1vw6w6135g43ku6%2F Abstract  Primary undifferentiated carcinoma of the small intestine is an extremely rare neoplasm. Here, we report a case of primary undifferentiated carcinoma that arose from the ileum in a 65-year-old woman. Laboratory data revealed anemia and slightly elevated inflammatory parameters. Computed tomography showed a heterogeneous mass in the pelvic cavity, and magnetic resonance imaging revealed that the margin of the tumor mass was clear. Positron emission tomography using 18F-fluoro-2-deoxy-d-glucose (FDG) showed accumulation of FDG on the tumor mass with a standardized uptake value of 8.3. Partial resection of the ileum to remove the tumor was performed under a clinical diagnosis of small intestinal carcinoma. The tumor was nodulated and had a circumscribed margin 6.5 × ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822401 Tue, 03 Aug 2010 17:01:10 +0100 3822401 http://www.medworm.com/index.php?rid=3822400&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw461685425562385%2F Abstract  Sex steroids play important roles in the development of human breast carcinoma. Androgen receptor (AR) is expressed in a majority of breast carcinoma tissues. However, the significance of androgen actions remains largely unclear in breast carcinoma, differing from estrogen actions. Therefore, in this review, we summarized recent studies on androgens in breast carcinoma. Concentration of a potent androgen, 5α-dihydrotestosterone (DHT), was significantly higher in breast carcinoma tissue than in plasma, and DHT is considered to be locally produced from circulating androstenedione by 17β-hydroxysteroid dehydrogenase type 5 and 5α-reductase. On the other hand, aromatase was recently reported as a negative regulator for intratumoral DHT production by possibly reducin... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822400 Tue, 03 Aug 2010 17:01:10 +0100 3822400 http://www.medworm.com/index.php?rid=3822402&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F54j1j8jr4086h6kx%2F Abstract  Since the concept of gene profile-based intrinsic subtypes was proposed, various studies on pathological characteristics have been performed. Particularly, triplenegative (TN) breast cancer, which is negative for all hormone receptors [estrogen receptor (ER) and/or progesterone receptor (PgR) and human epidermal growth factor 2 (HER2)], has been attracting attention because effects of endocrine and targeting therapies cannot be anticipated and thus selecting a treatment method is difficult. TN cancer accounts for about 10%–15% of all invasive breast cancer cases in Japanese, which is significantly lower than the incidence reported in the United States. Cytokeratin (CK) 5/6 or epidermal growth factor receptor (EGFR) is positive in 80%, being classified as basal-l... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822402 Tue, 03 Aug 2010 17:01:09 +0100 3822402 http://www.medworm.com/index.php?rid=3822403&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl57401wp74263j57%2F Abstract  Renal cell carcinoma (RCC) associated with Xp11.2 translocation/TFE3 gene fusion recently has been found. In this article, we demonstrate an unusual features of such a case. A 73-year-old Japanese woman presented with macroscopic hematuria. The imaging examinations disclosed the renal tumor. Histological examination showed the finding of ASPL-TFE3 RCC, which was characterized by papillary, alveolar, or solid growth of voluminous cell with clear and eosinophilic cells, and stromal psammoma body and hyaline nodules. Additionally, shrunken nuclei, thick cell border, and perinuclear clearing characteristic of chromophobe renal cell carcinoma were observed in the alveolar growth area and the transitional zone between stromal hyalinization, and osseous metaplasia was ide... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822403 Tue, 03 Aug 2010 17:01:08 +0100 3822403 http://www.medworm.com/index.php?rid=3822404&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4410u1g5554228rt%2F Abstract  Tight junctions (TJs) function primarily as a barrier against paracellular transport between epithelial cells and are composed mainly of occludin (OLD) and claudins (CLDs). The CLD family consists of 24 members that show tissue- or cell-specific expression. Ameloblasts, which originate from the oral epithelium, form enamel, and enamel proteins and minerals are transported across the ameloblastic layer during amelogenesis. We immunohistochemically examined the distribution patterns of TJs in ameloblasts by observing the expression patterns of OLD and CLDs (CLD-1 to CLD-10). Secretory ameloblasts contained OLD and CLD-1, -8, and -9 at the distal end of the cell. In mature ameloblasts, OLD and CLD-1, -6, -7, -8, -9, and -10 were present mainly at both the distal and ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822404 Tue, 03 Aug 2010 17:01:07 +0100 3822404 http://www.medworm.com/index.php?rid=3822405&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk464833114177117%2F In conclusion, an adequate protocol for the cryopreservation of human primary cultured HSCs could be established. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0484-5Authors Anna Nakamura, Kurume University Research Center for Innovative Cancer Therapy 67 Asahi-machi Kurume 830-0011 JapanTakato Ueno, Kurume University Research Center for Innovative Cancer Therapy 67 Asahi-machi Kurume 830-0011 JapanYumihiko Yagi, Kurume University Research Center for Innovative Cancer Therapy 67 Asahi-machi Kurume 830-0011 JapanKoji Okuda, Kurume University Research Center for Innovative Cancer Therapy 67 Asahi-machi Kurume 830-0011 JapanToshiro Ogata, Kurume University Research Center for Innovative Cancer Therapy 67 Asahi-machi Kurume 830-0011 JapanToru Nakamura, Kurume... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822405 Tue, 03 Aug 2010 17:01:06 +0100 3822405 http://www.medworm.com/index.php?rid=3822406&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft576n13253p45665%2F Abstract  Our laboratory discovered nestin-expressing hair follicle stem cells and demonstrated their pluripotency. We have shown that nestin-positive and K15-negative multipotent hair follicle stem cells are located above the hair follicle bulge, and we termed these cells hair follicle pluripotent stem (hfPS) cells. We have previously shown that hair follicle stem cells can regenerate peripheral nerve and spinal cord. In the present study, we describe the embryonic development of the hair follicle stem cell area (hfPSCA), which is located above the bulge and below the sebaceous glands in the adult mouse. At embryonic day 16.5 (E16.5) of nestindriven GFP (ND-GFP) transgenic mice, which express nestin in hfPS cells, the ND-GFP hair follicle stem cells are located in mesenchy... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822406 Tue, 03 Aug 2010 17:01:04 +0100 3822406 http://www.medworm.com/index.php?rid=3822407&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj74j044352417j05%2F Abstract  Characterization of the signet-ring cell carcinoma (sig) component of a urothelial carcinoma (UC) in the urinary bladder of a 64-year-old man, obtained by transurethral resection of bladder tumor (TUR-BT), is reported. In the present case, a characteristic sig component was detected in approximately 20% of UC, G2 tissues. The sig cells were morphologically similar to those found in gastric cancers and were positively stained with periodic acid-Schiff reaction and Alcian blue and mucicarmine stains. Immunohistochemically, the sig cells were selectively positive for carcinoembryonic antigen (CEA), MUC2, and MUC5AC. These immunohistochemical characteristics were similar to those of sig cells in the stomach, except for the positivity with MUC2. It is interesting to no... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822407 Tue, 03 Aug 2010 17:01:03 +0100 3822407 http://www.medworm.com/index.php?rid=3822409&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh129260nk43tx544%2F Content Type Journal ArticleCategory Special Review Series: Breast Carcinoma and Steroid HormonesDOI 10.1007/s00795-010-0505-4Authors Takuya Moriya, Kawasaki Medical School Department of Pathology Kurashiki Japan Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 43 Journal Issue Volume 43, Number 2 / June, 2010 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822409 Tue, 03 Aug 2010 17:01:02 +0100 3822409 http://www.medworm.com/index.php?rid=3822408&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh02m4u0582154368%2F In conclusion, this is the first case of hybrid PNST reported to occur in the nasopharyngeal area. Pathologists should be aware of the possibility that hybrid PNST may present outside soft tissue. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0418-7Authors Naoto Kuroda, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanDmitry V. Kazakov, Charles University Hospital Plzen Department of Pathology Plzen Czech RepublicOndrej Hes, Charles University Hospital Plzen Department of Pathology Plzen Czech RepublicMichal Michal, Charles University Hospital Plzen Department of Pathology Plzen Czech RepublicMasakazu Goda, Kochi Red Cross Hospital Department of Otorhinolaryngology Kochi JapanKatsushi Miyazaki, Kochi ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3822408 Tue, 03 Aug 2010 17:01:02 +0100 3822408 http://www.medworm.com/index.php?rid=3410846&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn20t8j5124303013%2F Abstract  It is well known that the epididymis is responsible for the transportation, storage, and maturation of spermatozoa, and that it has four segments: i.e., initial segment, caput, corpus, and cauda. Previous studies revealed the presence of a very dense network of blood capillaries only in the initial segment; however, the relative distribution of blood and lymphatic capillaries has remained unknown. In the present study, we investigated the distribution of intraepididymal blood and lymphatic capillaries in mice by use of monoclonal antibodies against CD31 and lymph vessel endothelium HA-receptor 1, respectively. The results showed that lymphatic networks were quite scarce in the initial segment and strikingly abundant in the cauda compared with the other two regions... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410846 Thu, 25 Mar 2010 18:56:28 +0100 3410846 http://www.medworm.com/index.php?rid=3410845&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx8258380653598j6%2F We report a 17-year-old woman with refractory high-grade breast cancer who died early after surgery, with reference to the histogenesis of the cancer. Macroscopically, the tumor was cystic, composed of a mixture of solid and myxomatous areas. Histologically, the tumor exhibited ductal structures and areas with squamoid features. Cancer cells were markedly atypical and polymorphic, and included a mixture of bizarre and eosinophilic cells with rhabdoid feature-like free cells. Immunohistochemically, cytokeratin (CK) 8, CK 18, 34betaE12, CD10, involucrin, CK14, and vimentin were partially positive, whereas estrogen and progesterone receptors and HER-2 were negative. These findings suggest an undifferentiated cancer whose cells have multilineage potential to differentiate into mesenchyma... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410845 Thu, 25 Mar 2010 18:56:28 +0100 3410845 http://www.medworm.com/index.php?rid=3410847&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F65p321rj2077v780%2F We present three cases of MM of the peritoneum including autopsy results, in which factors related to long-term survival were investigated. Case 1 was a 69-year-old man who died 6 years after the initial diagnosis. In case 2, a 67-year-old woman came to us with abdominal distention and, despite chemotherapy, died 9 months after the initial diagnosis. The patient in case 3 was a 68-year-old man who also had abdominal distention and died 9 months after the initial diagnosis. We studied the clinicopathological appearance and performed immunohistochemical staining including Ki-67 labeling index (Ki-67 LI) in primary and metastatic sites of these cases. The histological findings of case 1 indicated epithelioid type; case 2 and 3 were of biphasic type. Immunohistochemical results were cons... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410847 Thu, 25 Mar 2010 18:56:27 +0100 3410847 http://www.medworm.com/index.php?rid=3410848&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd15r884w3t30w18m%2F In conclusion, immunoreactive IGF-II was detected in SFT that was not associated with hypoglycemia. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0451-1Authors Takashi Yuri, Kansai Medical University Department of Pathology II Moriguchi. Osaka JapanSayaka Kanematsu, Kansai Medical University Department of Pathology II Moriguchi. Osaka JapanYen-Chang Lei, Kansai Medical University Department of Pathology II Moriguchi. Osaka JapanMaki Kuwata, Kansai Medical University Department of Pathology II Moriguchi. Osaka JapanMasaharu Oishi, Kansai Medical University Takii Hospital Division of Surgery Osaka JapanAiro Tsubura, Kansai Medical University Department of Pathology II Moriguchi. Osaka Japan Journal Medical Molecular MorphologyOnline ISSN 1860-1499Pri... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410848 Thu, 25 Mar 2010 18:56:26 +0100 3410848 http://www.medworm.com/index.php?rid=3410850&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc575831k1g6kl8q8%2F Abstract  Because the progression and metastasis of solid tumors depend on their local microcirculation, we sought to characterize tumor angiogenesis three dimensionally in a highly metastatic mouse melanoma model, B16BL6 (B16), injected with Matrigel into the subcutis in the skin on the back of syngeneic C57BL/6 mice. We found that B16 with Matrigel grew significantly faster than B16 alone and had altered tumor angiogenesis. Tumor vessels apparently grew vigorously in the opposite direction of the tumor without invading the tumor mass until at least day 10 of injection. In addition, vascular branching resulted not only from sprouting as was seen in B16 without Matrigel but also from vascular splitting, either because of compression from outside the vessels or from septum f... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410850 Thu, 25 Mar 2010 18:56:25 +0100 3410850 http://www.medworm.com/index.php?rid=3410849&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv7115m6202641425%2F Abstract  Several human liver diseases are associated with formation of hepatocyte Mallory-Denk bodies (MDB) composed of keratins and ubiquitin. Similar inclusions are found in various other diseases, neurodegenerative and muscle disorders. However, the mechanisms of MDB formation have been unclear. Autophagy is a degradation process of intracellular proteins and organelles. In the present study we examined the association of autophagy with the formation of MDB. We fed wild-type and keratin 8-transgenic mice with a 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-containing diet for 9 days. The livers were analyzed by immunohistochemistry and conventional and immune electron microscopy. Short-term DDC feeding induced MDB in keratin 8-transgenic but not in nontransgenic mouse... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410849 Thu, 25 Mar 2010 18:56:25 +0100 3410849 http://www.medworm.com/index.php?rid=3410852&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk5j39565367m8hg6%2F In conclusion, elemental mapping by SRXRF microscopy was a highly sensitive method for the detection and mapping of elements such as iron and copper in liver sections. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0474-7Authors Hisoka Kinoshita, Kobe University Graduate School of Medicine Departments of Surgery, Division of Hepato-Biliary-Pancreatic Surgery Kobe 650-0017 JapanYuichi Hori, Kobe University Graduate School of Medicine Departments of Surgery, Division of Hepato-Biliary-Pancreatic Surgery Kobe 650-0017 JapanTakumi Fukumoto, Kobe University Graduate School of Medicine Departments of Surgery, Division of Hepato-Biliary-Pancreatic Surgery Kobe 650-0017 JapanTakuji Ohigashi, Ritsumeikan University Research Organization of Science & Engineering Ky... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410852 Thu, 25 Mar 2010 18:56:24 +0100 3410852 http://www.medworm.com/index.php?rid=3410851&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu25130234g576u6w%2F Abstract  We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410851 Thu, 25 Mar 2010 18:56:24 +0100 3410851 http://www.medworm.com/index.php?rid=3410853&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3l360h2748g6j51w%2F Abstract  Solid papillary carcinoma is a rare breast lesion, but this entity remains poorly recognized. In this article, we report a case of solid papillary carcinoma of the breast with focus on cytological and histological findings. A 66-year-old Japanese woman presented with nipple discharge. Imprint cytology obtained from the surgically resected breast specimens showed a plasmacytoid appearance and spindle cell morphology with low-grade atypia. Histologically, the tumor revealed findings of solid papillary carcinoma and predominantly showed an intraductal lesion with focal minimal invasion into the breast parenchyma. Fibrovascular cores with hyalinization were seen. Proliferation of short spindle cells was also focally observed. Pseudorosette formation or nuclear palisad... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410853 Thu, 25 Mar 2010 18:56:23 +0100 3410853 http://www.medworm.com/index.php?rid=3410854&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj3vr86503125756n%2F This study aimed to clarify the localization of AQP-1 in the microvessels in normal and cirrhotic rat liver. To establish a rat cirrhosis model, thioacetamide (TAA) was injected for 24 weeks. AQP-1 in liver specimens was examined by immunohistochemistry (IHC), Western blotting, and immunoelectron microscopy (IEM). IHC revealed that AQP-1 was localized in hepatic sinusoids, especially on the liver sinusoidal endothelial cells (LSECs), predominantly in zone 1 in control rats, whereas AQP-1 immunoreactivity was increased on LSECs in central portions of regenerative nodules in cirrhotic rats, and was expressed especially strongly on the outer side of the duplicated liver cell cords. IEM demonstrated that, in control livers, AQP-1 was mainly expressed on the plasma membrane of LSECs in zo... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410854 Thu, 25 Mar 2010 18:56:22 +0100 3410854 http://www.medworm.com/index.php?rid=3410855&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F22014361107j3300%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00795-010-0491-6Authors Jeng-Dong Hsu, Chung Shan Medical University Hospital Department of Pathology Taichung TaiwanChih-Ping Han, Chung-Shan Medical University Hospital Department of Obstetrics and Gynecology No. 110, Sec. 1, Chien-Kuo N. Road Taichung Taiwan, ROC Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 43 Journal Issue Volume 43, Number 1 / March, 2010 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3410855 Thu, 25 Mar 2010 18:56:21 +0100 3410855 http://www.medworm.com/index.php?rid=3121998&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft225143613630868%2F Abstract  Junctional epithelium, a nonkeratinized stratified epithelium, extends apically in apposition to the surface of the enamel to form a seal between the epithelium and the tooth. Desmosomes and gap junctions adhere to the junctional epithelium through cell-cell contact, but no evidence of tight junctions has been found. Recently, tight junction hallmark proteins and tight junction-related structures have been identified in stratified squamous epithelium. The present study examined whether tight junction proteins were expressed in the junctional epithelium. We used immunohistochemical techniques to observe expression of claudin-1, -4, -5, -7, and occludin in porcine gingival junctional epithelium. Claudin-4 exhibited immunoreactivity in the intercellular spaces of all... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3121998 Wed, 23 Dec 2009 23:03:19 +0100 3121998 http://www.medworm.com/index.php?rid=3121997&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F317861u601kqm527%2F Abstract  Gangliocytic paraganglioma (GPG) is a rare tumor, occurring almost exclusively in the duodenum. In the present case, a submucosal tumor 2.5 × 2 × 1.5 cm in size was located on the anal side of the papilla of Vater, with clear margins and without capsule on cut-surface examination. Tumor cells included three types of cells: ganglion-like cells (GCs), endocrine cells (ECs), and Schwann cells (SCs). The GCs were large with eccentric nuclei with large nucleoli and clear abundant cytoplasm. ECs were detected in small nests, surrounded by bundles of SCs. Immunohistochemically, GCs were positive for synaptophysin, neuron-specific enolase (NSE), and CD56. ECs were positive for chromogranin A, NSE, somatostatin, pancreatic polypeptide, and CD56, and were associated with ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3121997 Wed, 23 Dec 2009 23:03:19 +0100 3121997 http://www.medworm.com/index.php?rid=3122000&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2t5263623ww844l8%2F Abstract  Tricellulin was identified as the first marker of the tricellular tight junction, which forms at the meeting points of three cells, and it is required for the maintenance of the transepithelial barrier. Although it is also considered to be important for the mucosal barrier of the upper respiratory tract, little is known about its expression and localization. In the present study, we examined the expression and localization of tricellulin in normal human nasal epithelial cells in vivo and in vitro, especially using primary cultures and telomerase reverse transcriptase (hTERT)-transfected cells. In human nasal epithelial cells in vivo and in vitro, mRNA and protein of tricellulin were detected. It was localized not only at tricellular contacts but also at bicellular... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122000 Wed, 23 Dec 2009 23:03:18 +0100 3122000 http://www.medworm.com/index.php?rid=3121999&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa51014326j6x65q5%2F Abstract  We previously established an experimental model of autoimmune orchitis (EAO) by means of immunization with testicular germ cells (TGC) alone in mice and confirmed that the disease can be transferred to mice that had received CD4+ but not CD8+ or B lymphocytes obtained from TGC-immunized donor mice. The tubuli recti (TR) are special in that lymphocytes first accumulate around them before spreading to the peripheral seminiferous tubules in EAO. However, the minute changes in the TR remain unknown. Therefore, we investigated the histopathology of the TR before the induction of spermatogenic disturbance. The results revealed that the first infiltrating lymphocytes around the TR were not only of T-cell but also of B-cell lineage. Moreover, it was also shown that some o... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3121999 Wed, 23 Dec 2009 23:03:18 +0100 3121999 http://www.medworm.com/index.php?rid=3122002&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy18431641q572067%2F Abstract  It is well established that hyperinsulinemia, resulting from insulin resistance, plays a role in the pathophysiology of polycystic ovary syndrome (PCOS). The aim of this study was to investigate if ovarian follicular development and atresia are impaired in obese hyperinsulinemic (fa/fa) Zucker rats. To gain insight into the molecular mechanism of follicular atresia, we also examined the expression and localization of forkhead transcription factor FOXO1, a major regulator of cell fate decisions such as differentiation, cell-cycle arrest, and cell death. Serum insulin but not gonadotropin levels were significantly higher in obese (fa/fa) rats when compared to lean controls. Total ovarian follicle number and the percentage of atretic follicles were also significantly... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122002 Wed, 23 Dec 2009 23:03:13 +0100 3122002 http://www.medworm.com/index.php?rid=3122001&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8v16p7657pp74524%2F Abstract  One of the most common diseases of the pancreas is diabetes mellitus. The current treatment of exogenous insulin supply is not fully capable of achieving tight control of glucose regulation, leading to long-term complications. Hence, recent success in islet transplantation-based therapies for diabetes mellitus and the extreme shortage of pancreatic islets have motivated recent efforts to develop renewable sources of islet-replacement tissue. Of clinical interest, I review the recent progress on stem cell-based strategies for diabetes in view of regenerative medicine. Content Type Journal ArticleCategory ReviewDOI 10.1007/s00795-009-0471-xAuthors Yuichi Hori, Kobe University Graduate School of Medicine Department of Surgery, Division of Hepato-Biliary-Pancreatic... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122001 Wed, 23 Dec 2009 23:03:13 +0100 3122001 http://www.medworm.com/index.php?rid=3122004&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ffg6081qv6m6250q1%2F We examined the expressions of adhesion molecules (E-cadherin, β-catenin, CD44s, and CD44v6) and Ki-67 labeling index (Ki-67 LI) in low- and moderate-grade dysplasia and invasive carcinoma components in ten noninvasive intraductal papillary mucinous neoplasms (IPMNs) of the pancreas and eight invasive carcinomas associated with IPMNs of the pancreas using immunohistochemical methods. There was no significant difference in regard to the proportion of components expressing either E-cadherin or β-catenin in more than 70% of the tumor cells between the low- and moderate-grade dysplasia components. In contrast, the proportion of those in invasive carcinoma components was significantly lower than in low- or moderate-grade dysplasia components. Also, there was no significant difference in... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122004 Wed, 23 Dec 2009 23:03:12 +0100 3122004 http://www.medworm.com/index.php?rid=3122003&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ffkx0h18226381w03%2F We report a case with focus on immunocytochemistry of imprint cytological specimens here. A 43-year-old Japanese man presented with cough. Brushing cytology of the tumor using a bronchoscope showed a malignant neoplasm. Subsequently, left lower lobectomy was undertaken. Imprint cytological material of the neoplastic cells noted some clusters of round, or oval, to polygonal epithelioid cells. Histological examination showed the proliferation of epithelioid neoplastic cells containing erythrocytes in the cytoplasm. The immunocytochemistry in imprint cytological specimens showed a focal positive reaction for von Willebrand factor, and the immunohistochemistry of the surgically resected lung tumor showed a focal positive reaction for Fli-1, von Willebrand factor, CD31, and CD34. Finally... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122003 Wed, 23 Dec 2009 23:03:12 +0100 3122003 http://www.medworm.com/index.php?rid=3122005&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F70p18627173v0640%2F Abstract  IgG4-related disease has been recently described. This disease occurs in various anatomic locations including pancreas, biliary tract, liver, retroperitoneum, kidney, breast, lung, thyroid gland, prostate, salivary gland, lacrimal gland, and lymph node. In this article, we report the first case of IgG4-related disease arising in the renal pelvis. A 49-year-old Japanese woman was found to show left hydronephrosis by a medical checkup. Histological examination of the renal pelvic tumor showed IgG4-related disease. Her postoperative serum IgG4 was elevated, and this was compatible with IgG4-related disease. Systemic examination showed swelling of major and minor salivary glands and the lacrimal glands, and biopsy of the minor salivary gland revealed the finding of Ig... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122005 Wed, 23 Dec 2009 23:03:09 +0100 3122005 http://www.medworm.com/index.php?rid=3122006&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxm41045228464525%2F Abstract  This review article covers the molecular mechanisms of secretory granule formation by chromogranin transfection. Recently, a few investigators have reported that the transfection of chromogranin A and B produces the structures of secretory granules. We used the GFP-chromogranin A transfection method to nonendocrine cells, COS-7 cells, which are not equipped with secretory granules. Despite the absence of endogenous secretory granules in nontransfected COS-7 cells, COS-7 cells transfected with chromogranin A contained granule-like structures in electron micrographs. The granules were composed of an outer limiting membrane with core structures that were interpreted as secretory granules. Human chromogranin A (CgA) labeled with 5-nm gold particles was present in seve... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122006 Wed, 23 Dec 2009 23:03:08 +0100 3122006 http://www.medworm.com/index.php?rid=3122007&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn2703m1158h233w6%2F We report a SFT of the left kidney that presented as a malignant tumor on radiographic findings in a 75-year-old Japanese woman. The tumor was well circumscribed and composed of a mixture of spindle cells and dense collagenous bands with no areas of necrosis or cystic changes noted macroscopically or microscopically. Electron microscopy showed fibroblast-like cells with well-developed rough endoplasmic reticulum, surrounded with collagen fibers. Immunohistochemistry revealed reactivity for vimentin, CD34, Bcl-2, and CD99, but no staining for cytokeratin, S-100, desmin, actin, D2-40, or epithelial membrane antigen (EMA). These findings were compatible with those of SFT. Although SFT of the kidney is extremely rare, this tumor must be included in the differential diagnosis when we enco... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3122007 Wed, 23 Dec 2009 23:03:06 +0100 3122007 http://www.medworm.com/index.php?rid=2850930&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2160735t75255418%2F Abstract  Imaging mass spectrometry (IMS) is a two-dimensional mass spectrometry to visualize the spatial distribution of biomolecules that does not need either separation or purification of target molecules and enables us to monitor not only the identification of unknown molecules but also the localization of numerous molecules simultaneously. Among the ionization techniques, matrix-assisted laser desorption/ionization (MALDI) is one of those most generally used for IMS, which allows the analysis of numerous biomolecules ranging over wide molecular weights. At present, targets of IMS research have expanded to the imaging of small endogenous metabolites such as lipids, exogenous drug pharmacokinetics, exploring new disease markers, and other new scientific fields. Content... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850930 Tue, 29 Sep 2009 01:07:05 +0100 2850930 http://www.medworm.com/index.php?rid=2850931&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9r35784685354432%2F We report a 21-year-old man with a primitive neuroectodermal tumor (PNET) of the kidney that was difficult to distinguish from other small round cell tumors, e.g., small cell carcinoma, malignant lymphoma, and Wilms’ tumor. Pathologically, a primitive rosette-like pattern was shown by H&E staining; expression of MIC-2 was indicated by an immunohistochemical test; rather primitive organelles were observed by an ultrastructural method; and translocation of chromosome 22 was confirmed by FISH. We therefore diagnosed the current case as PNET. The patient had undergone a right radical nephrectomy more than 1.5 years earlier. After neither metastases nor recurrences for 0.5 year, imaging examinations revealed masses in his liver. He received chemotherapy and underwent surgery again, but ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850931 Tue, 29 Sep 2009 01:07:04 +0100 2850931 http://www.medworm.com/index.php?rid=2850934&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F043681848115042x%2F Abstract  In the process of ovary sexual maturation, most immature ovarian follicles degrade into atretic follicles accompanied by apoptosis in granulosa cells. Macrophages can recognize apoptotic cells through specific binding with phosphatidylserine (PS), exposed on the surface of apoptotic cells, which is mediated by milk-fat globule-EGF factor 8 (MFG-E8). In the present research, we examined the involvement of the MFG-E8-dependent phagocytosis system in the atretic follicles of developing mouse ovaries. The number of atretic follicles and DNA-fragmented granulosa cells significantly increased in B6C3F1 mice during 2 to 6 weeks. Chromatin-condensed granulosa cells were engulfed by macrophages, which existed in the stroma or atretic follicles, or by neighboring normal gra... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850934 Tue, 29 Sep 2009 01:07:03 +0100 2850934 http://www.medworm.com/index.php?rid=2850933&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F872t7547r6956n38%2F Abstract  An extremely rare case of mixed acinar-endocrine carcinoma (MAEC) arising from the stomach in a 56-year-old Japanese woman is herein presented. An endoscopic examination and computed tomography showed a protruding gastric tumor and a large extragastric mass, respectively. Macroscopic observation on the surgical specimen revealed the extragastric cystic mass was continued to the intragastric tumor. Histologically, the intragastric tumor consisted of large or small solid nests with acinar appearance. The cancer cells had an ovoid nuclei and polygonal cytoplasm, which was frequently amphophilic. Immunohistochemical examination showed that the cancer cells were positive for chromogranin-A, synaptophysin, alpha-amylase, lipase, and alpha-fetoprotein (AFP) but were nega... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850933 Tue, 29 Sep 2009 01:07:03 +0100 2850933 http://www.medworm.com/index.php?rid=2850936&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fptl43u875227h721%2F Abstract  Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers worldwide. The main HCC-associated diseases are chronic infections with hepatitis B virus (HBV) and hepatitis C virus (HCV), and HBV-associated HCC is still prevalent in Asia. Many studies have suggested that HBV X protein (HBX), which is the most common ORF integrated into the host genome, plays a crucial role in hepatocarcinogenesis. However, the accumulated evidence regarding HBX-mediated signaling pathways is not concordant, and it is difficult to understand the mechanistic nature of HBX-associated hepatocarcinogenesis. For example, HBX was reported to inactivate the early responses to DNA damage via p53-dependent and -independent pathways by interacting with several DNA damage-bind... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850936 Tue, 29 Sep 2009 01:07:02 +0100 2850936 http://www.medworm.com/index.php?rid=2850935&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy08316466n848485%2F In conclusion, we demonstrate that OPN is widely distributed in normal human cells, particularly those comprising the central and peripheral nervous systems. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0459-6Authors Yasuto Kunii, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanShin-ichi Niwa, Fukushima Medical University School of Medicine Department of Neuropsychiatry Fukushima JapanYoshiaki Hagiwara, Immunobiological Laboratories Takasaki JapanMasahiro Maeda, Immunobiological Laboratories Takasaki JapanTsutomu Seitoh, Immunobiological Laboratories Takasaki JapanToshimitsu Suzuki, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 Japan ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850935 Tue, 29 Sep 2009 01:07:02 +0100 2850935 http://www.medworm.com/index.php?rid=2850937&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fnx1g54008t60x904%2F Abstract  A case of extraskeletal myxoid chondrosarcoma (ESMC), which developed in the right thigh of a middle-aged Japanese woman, was studied using immunohistochemistry, conventional electron microscopy, and the quick-freezing and deep-etching (QF-DE) method. In addition to typical light microscopic findings of ESMC, conventional electron microscopy indicated that the tumor cells had features of chondrocytes. Immunohistochemically, the tumor cells showed a positive immunoreaction for S100 protein. A diagnosis of ESMC was made. An interesting observation was the ultrastructural features of collagen fibrils in the myxoid matrix highlighted by the QF-DE method. These collagen fibrils consisted of relatively thin collagen (20–35 nm) with pleated surface structures. The surf... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850937 Tue, 29 Sep 2009 01:07:00 +0100 2850937 http://www.medworm.com/index.php?rid=2850939&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6506mq30px1331t2%2F We report here a case of HTT with cytological, immunohistochemical, and ultrastructural findings, focused in particular on the hyalinizing mass. Cytologically, tumor cells exhibiting many intranuclear cytoplasmic inclusions and nuclear grooves were found in association with light green-positive, irregular, fluffy membranous structures on touch smear. Staining with antibody to collagen type IV was positive in these membranous structures. Histopathologically, tumor cells exhibited many intranuclear cytoplasmic inclusions, and were positive for staining with antibodies to S100 protein, neuron-specific enolase, thyroglobulin, and vimentin. The hyalinizing eosinophilic mass, which was positive for PAS reaction, and for staining by antibody to collagen type IV, gradually increased in the a... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850939 Tue, 29 Sep 2009 01:06:59 +0100 2850939 http://www.medworm.com/index.php?rid=2850938&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe321v66u21k7826w%2F We report here a case of CCC. A 67-year-old Japanese man presented with right arthralgia for 1 year, and histological examination of the subsequent surgical resection of the right femoral bone showed the finding of CCC. Ultrastructurally, most organelles were observed in the perinuclear area. Clear neoplastic cells contained many glycogen particles in the area of the cytoplasm lacking organelles, although glycogen particles overall seemed to be evenly distributed in the cytoplasm. Some mitochondria, Golgi complex, actin-like filaments, and rough endoplasmic reticulum were also demonstrated in the cytoplasm of clear cells. Well-developed microvilli were also seen on the surface of neoplastic cells. These structures in neoplastic cells corresponded notably to structures of normal chond... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850938 Tue, 29 Sep 2009 01:06:59 +0100 2850938 http://www.medworm.com/index.php?rid=2850940&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl17p502527467275%2F Abstract  Nodal staging is a crucial factor in choosing the treatment option for non-small cell lung cancer (NSCLC). However, so far as we know, a computer-based histomorphometric analysis of lymph nodes in NSCLC has never been developed. We studied 299 surgically resected lymph nodes from 108 patients with NSCLC. Microscopic digital images were analyzed with Scion Image software. Seventy lymph nodes had at least one metastatic focus. The metastasis occupancy area per node ranged from 0.01 to 209.58 mm2 (mean, 29.58 ± 5.87 mm2). The metastasis occupancy ratio ranged from 0.01% to 100% (mean, 48.70% ± 42.10%). The short-axis diameter of malignant lymph nodes was significantly longer than that of benign lymph nodes (P = 0.0002). The average metastasis occupancy area in the re... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850940 Tue, 29 Sep 2009 01:06:57 +0100 2850940 http://www.medworm.com/index.php?rid=2850941&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc602248864075553%2F Abstract  A recent study of a rat model treated with grafted collagen filament (CF) after spinal cord transection showed dramatic recovery of motor function but did not report on the acute-stage phenomenon. In the present study, we describe molecular and histological aspects of the axonal regeneration process during the acute stage following spinal cord transection. The spinal cord of 8-week-old rats was completely transected, and a scaffold of almost the same size as the resected portion was implanted in the gap. Changes in the mRNA expression of four neurotrophic factors [nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), NT-3, and glial cell-derived neurotrophic factor (GDNF)] were analyzed after 72 h. The expression of BDNF and NT-3 mRNA increased sign... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2850941 Tue, 29 Sep 2009 01:06:56 +0100 2850941 http://www.medworm.com/index.php?rid=2504317&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp845670673001q20%2F In conclusion, ET-1 and ET-B are expressed with differentiation of neuroblastic tumors. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0437-4Authors Nobuo Hoshi, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanTakashi Sugino, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanToshimitsu Suzuki, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 Japan Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 42 Journal Issue Volume 42, Number 2 / June, 2009 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504317 Thu, 18 Jun 2009 16:38:38 +0100 2504317 http://www.medworm.com/index.php?rid=2504318&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxv2g232v6353656m%2F Abstract  To address whether adult rat ventricular cardiomyocytes (ARVCs) exposed to oxidant stress die via apoptosis (secondarily by necrosis) or primarily by necrosis, we exposed ARVCs to hydrogen peroxide (H2O2; 0.1–100 μM) for up to 24 h and then compared them with isoproterenol-induced apoptotic and Triton X-induced necrotic controls. Cellular shrinkage preceded plasma membrane disruption, reflected by trypan blue uptake in ARVCs exposed to lower concentrations of H2O2 (<1 μM; an apoptotic pattern), but the order was reversed in cells exposed to higher concentrations of H2O2 (>1 μM; a necrotic pattern). DNA fragmentation, caspase-3 activation, mitochondrial membrane potential preservation, and ATP preservation were all apparent in ARVCs treated with low H2O2 (... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504318 Thu, 18 Jun 2009 16:38:35 +0100 2504318 http://www.medworm.com/index.php?rid=2504319&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd81l4q8228h251k6%2F Abstract  Human immunodeficiency virus type 1 Rev (regulator of the expression of the virion) protein was shown to reduce the expression level of the co-transfected luciferase reporter gene (luc+) introduced to monitor transfection efficiency. We studied the mechanism of the inhibitory Rev effect. The effect, caused by nuclear retention of luc+ mRNA, was reversed if rev had a point mutation that makes its nuclear export signal (NES) unable to associate with cellular transport factors. The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export. This finding was also supported by the overexpression of ΔCAN, another specific CRM1 inhibitor that caused inhibition of luciferase gene expression. Experiments involving tsBN... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504319 Thu, 18 Jun 2009 16:38:30 +0100 2504319 http://www.medworm.com/index.php?rid=2504321&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fvh6345337p174140%2F Abstract  Changes in the expression of E-cadherin have been reported to be important in the tumorigenesis and progression of epithelial ovarian carcinoma. To further examine the mechanisms regulating E-cadherin expression in ovarian tumorigenesis, we investigated the immunohistochemical expression of transcriptional repressors for E-cadherin, such as Snail, Slug, SIP1, and Twist, in the ovarian surface epithelium (OSE) and 95 cases of epithelial ovarian tumors. OSE cells were negative for SIP1 and Slug, whereas weak expression of Snail and Twist was observed in 8 (73%) and 3 (27%) cases, respectively. Of 95 ovarian tumors, the expression of Snail, Slug, SIP1, and Twist increased stepwise in benign, borderline, and malignant tumors. Among them, the expression of Snail showed... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504321 Thu, 18 Jun 2009 16:38:29 +0100 2504321 http://www.medworm.com/index.php?rid=2504320&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F581n0546r6x3p76t%2F Abstract  Arylsulfatase A (ARSA) and B (ARSB) have been regarded as lysosomal enzymes because of their hydrolytic activity on synthetic aromatic substrates and the lysosomal localization of their enzymatic activity. Using sea urchin embryos, we previously demonstrated that the bulk of ARS is located on the cell surface of the epithelium, colocalizing with sulfated polysaccharides, and that it does not exhibit enzymatic activity. To examine whether ARSA and ARSB exist on the cell surface in mammalian tissues, we raised antibodies against ARSA and ARSB and examined immunohistochemically their localization in the liver using light and electron microscopy. Here we show that mammalian ARSA and ARSB exist on the cell surface of sinusoidal endothelial cells, hepatocytes, and sinus... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504320 Thu, 18 Jun 2009 16:38:29 +0100 2504320 http://www.medworm.com/index.php?rid=2504322&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl04015k826073147%2F We present a case of hepatoid carcinoma of the abdominal skin in a male Wistar rat. Histopathologically, this carcinoma resembled human hepatocellular carcinoma with respect to trabecular-sinusoidal structures. Carcinoma tissues contain numerous eosinophilic globules and crystals, and in this case, we found the characteristic eosinophilic globules in the hepatoid carcinoma cells and the crystals in the extracellular portions. Vivid carcinoma cells full of eosinophilic globules were present near the necrotic areas in tumor tissue, wherein quadrate crystals unstained with eosin were observed. PAS staining after diastase digestion revealed that the globules were PAS positive and diastase resistant. In addition, we found that the hepatoid carcinoma cells were immunoreactive for α-1-anti... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504322 Thu, 18 Jun 2009 16:38:25 +0100 2504322 http://www.medworm.com/index.php?rid=2504323&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu14mx98n8n22v48m%2F Abstract  To evaluate the peculiar “inside-out” pattern in micropapillary (MP) carcinoma, we investigated the usefulness of KL-6 antibody in the assessment of the MP pattern of cancers, in comparison with antibodies to epithelial membrane antigen (EMA), MUC1 (CD227), and CD 10. Immunohistochemical investigation was performed on specimens exhibiting an MP pattern obtained from 12 persons with cancer: 4 with breast carcinoma, 3 with carcinoma of the urinary bladder, and 5 with colonic carcinoma. Immunohistochemical study with KL-6, EMA, and MUC1 antibodies revealed similar continuous linear positive patterns restricted to the surface of the MP pattern in both breast and urinary bladder cancers, revealing the peculiar “inside-out” morphology. However, EMA also gave cyt... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504323 Thu, 18 Jun 2009 16:38:22 +0100 2504323 http://www.medworm.com/index.php?rid=2504324&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk868358g56x48734%2F In this study, we analyzed the expression of mature miRNAs in esophageal squamous cell carcinoma (ESCC). The expression of 73 miRNAs was quantified by qRT-PCR in 30 primary ESCC specimens. We examined the correlation between miRNA expressions and the clinicopathological factors and prognosis of ESCC. The Kaplan-Meier survival curves showed that the high expression levels of 6 of the 72 miRNAs correlated with significantly lower patient survival rates. The overexpression of miR-129 was identified as a significant and independent prognostic factor (P = 0.031) in surgically treated ESCC patients. The hazard ratio for the prediction of early death was 18.11 for high versus low expression levels of miR-129. Similar results were obtained from an analysis performed on an additional 19 patie... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504324 Thu, 18 Jun 2009 16:38:17 +0100 2504324 http://www.medworm.com/index.php?rid=2504325&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb2338n4gvh110x45%2F Abstract  Recently, the concept of basal-like carcinoma has been proposed. However, there are only a few reports about the relationship between triple-negative cancer and basal-like carcinoma. In this article, we report the study of the expression of basal cell markers in 11 triple-negative cancers. Eight tumors (4 metaplastic carcinomas, 2 invasive ductal carcinomas, 1 invasive papillary carcinoma, and 1 medullary carcinoma) were positive for more than three markers among cytokeratins 5, 14, and 17, and p63. Three tumors (2 invasive ductal carcinomas and 1 apocrine carcinoma) were completely negative for all markers. Among 8 tumors positive for basal markers, cytokeratins 5 and 17 were expressed in all 8 tumors, cytokeratin 14 in 6 tumors, and p63 in 7 tumors. Finally, we ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2504325 Thu, 18 Jun 2009 16:38:05 +0100 2504325 http://www.medworm.com/index.php?rid=2402599&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe77742t332253717%2F In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by V. cholerae. The secretion and production of V. cholerae treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated V. cholerae but does so more slowly in neutral- and acidic-pH-treated V. cholerae. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in V. cholerae treated at alkaline pH for more than 10 min. The pH t... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2402599 Wed, 18 Mar 2009 06:49:34 +0100 2402599 http://www.medworm.com/index.php?rid=2283671&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmm1118p997054026%2F Abstract  A pleomorphic adenoma (PA) is the most common epithelial tumor in the salivary glands, but it frequently shows a mesenchyme-like histology, including the presence of myxoid and chondroid areas. Cartilage-specific matrix proteins are deposited in PA. Aggrecan is a major component of cartilage-specific proteoglycans. The present study examined the ultrastructure of the stromal areas in ten salivary PA specimens and investigated the distribution of aggrecan by immunoelectron microscopy. Aggrecan was deposited in the myxoid and chondroid stroma of PA. Ultrastructural observations revealed many proteoglycan cores and fibrils in the myxoid stroma and some spindle-shaped neoplastic myoepithelial cells with vacuoles and actin filaments in the myxoid areas. By immunoelectr... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283671 Wed, 18 Mar 2009 06:49:34 +0100 2283671 http://www.medworm.com/index.php?rid=2283669&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe77742t332253717%2F In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by V. cholerae. The secretion and production of V. cholerae treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated V. cholerae but does so more slowly in neutral- and acidic-pH-treated V. cholerae. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in V. cholerae treated at alkaline pH for more than 10 min. The pH t... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283669 Wed, 18 Mar 2009 06:49:34 +0100 2283669 http://www.medworm.com/index.php?rid=2283675&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv410j8401r161141%2F In this study, normal and hypoxic mouse kidneys were prepared using an “in vivo cryotechnique” (IVCT) and examined immunohistochemically with specific antibodies against phospho-(Ser/Thr) PKA/C substrate (P-PK-S) and phospho-(Ser/Thr) Akt substrate (P-Akt-S) to capture their time-dependent regulation in vivo. Left kidneys were cryofixed with IVCT under normal blood circulation and after varying hypoxic intervals, followed by freeze-substitution with acetone containing paraformaldehyde. Deparaffinized sections were immunostained for P-PK-S, Na+/HCO3 − cotransporter NBC1, and a membrane skeletal protein, 4.1B. The P-PK-S was diffusely immunolocalized in the cytoplasm of the proximal tubules in normal kidneys, whereas NBC1 and 4.1B were detected at the basal striations of S1 and ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283675 Wed, 18 Mar 2009 06:49:33 +0100 2283675 http://www.medworm.com/index.php?rid=2283673&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd680821449748x51%2F Abstract  The Tian-Long (TL) compound is a water-soluble extract of six Chinese medicinal herbs. To explore its antitumor properties and the mechanism for activity in gynecological malignancies, the present studies were carried out using Ishikawa cells derived from uterine endometrial adenocarcinoma. Morphologically, cell death and decrease in the number of viable cells were observed in the presence of the TL compound. The proliferation of Ishikawa cells was significantly suppressed in a time- and dose-dependent manner, as indicated by both the WST-1 and the BrdU incorporation assay. Results from both the WST-1 and the BrdU incorporation assay demonstrated that the compound could inhibit the cell proliferation despite the presence of 17β-estradiol in the medium. It is gene... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283673 Wed, 18 Mar 2009 06:49:33 +0100 2283673 http://www.medworm.com/index.php?rid=2283677&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl383181477234388%2F In this study, we solved the problem by our recently developed nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based imaging mass spectrometry (IMS). We revealed that the level of sulfatide in the middle molecular layer was significantly higher than that in granule cell layers and the inner molecular layer in the dentate gyrus of rat hippocampus. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0427-6Authors Hiroshi Ageta, Mitsubishi Kagaku Institute of Life Sciences (MITILS) Tokyo JapanSayaka Asai, Mitsubishi Kagaku Institute of Life Sciences (MITILS) Tokyo JapanYuki Sugiura, Mitsubishi Kagaku Institute of Life Sciences (MITILS) Tokyo JapanNaoko Goto-Inoue, Hamamatsu University School of Medicine Department of Molecular Anatomy 1-20-1 Handay... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283677 Wed, 18 Mar 2009 06:49:32 +0100 2283677 http://www.medworm.com/index.php?rid=2283679&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4867qw0145n33576%2F Abstract  Among various effector proteins for the Rho small GTPase, the function(s) of Rhotekin is almost unknown. We have identified a multi-domain adaptor protein, vinexin, as a binding partner for Rhotekin, using yeast two-hybrid screening of a human heart library. Rhotekin was found to associate with vinexin in vitro, in COS7 cells, and in brain tissues. The C-terminal Pro-rich motif of Rhotekin exhibited binding to the third SH3 domain of vinexin. The binding was little affected by Rho but was inhibited by activated Cdc42 in COS7 cells. Immunofluorescence analyses revealed partial colocalization of vinexin-α with Rhotekin at focal adhesions in REF52 fibroblast cells. These results suggest that Rhotekin forms a complex with vinexin and may play a role at focal adhesion... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283679 Wed, 18 Mar 2009 06:49:30 +0100 2283679 http://www.medworm.com/index.php?rid=2283681&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft1v86tg371l87h73%2F Abstract  The liver is a distinctive immune organ with predominant innate immunity, being rich in innate immune cells such as natural killer (NK) cells. In humans, NK cells comprise about 30%–50% of intrahepatic lymphocytes, whereas peripheral blood lymphocytes contain about 5%–20% NK cells. Accumulating evidence suggests that NK cells play an important role not only in host defense against invading microorganisms and tumor transformation in the liver but also in liver injury and repair. In recent years, significant progress has been made in terms of understanding how NK cells recognize their target cells and carry out their effector functions. It is now clear that NK cells are strictly regulated by numerous activating and inhibitory NK cell receptors that recognize var... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283681 Wed, 18 Mar 2009 06:49:29 +0100 2283681 http://www.medworm.com/index.php?rid=2283680&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb1822np4w42616g7%2F In conclusion, we suggest that endocrine cells in the ureteral urothelial epithelium may be precursor cells of ureteral small cell carcinoma. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-007-0384-5Authors Naoto Kuroda, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanKazunobu Katto, Kochi Red Cross Hospital Department of Urology Kochi JapanMasato Tamura, Kochi Red Cross Hospital Department of Urology Kochi JapanTomoyuki Shiotsu, Kochi Red Cross Hospital Department of Urology Kochi JapanKaori Inoue, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanMasahiko Ohara, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-85... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283680 Wed, 18 Mar 2009 06:49:29 +0100 2283680 http://www.medworm.com/index.php?rid=2283682&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj54206725q1017nm%2F Abstract  A rare case of neuroendocrine small cell carcinoma (SmCC) of the breast is reported. A 51-year-old postmenopausal woman noticed a nodule approximately 3 cm in diameter in her right upper breast. Histologically, the tumor consisted of small ovoid to pleomorphic cells with hyperchromatic nuclei, and a large central area was occupied by acellular amorphous tissue. Extensive lymphatic permeation was seen around the tumor. Invasive and in situ ductal carcinoma foci were not observed in and surrounding the tumor. Immunohistochemically, estrogen and progesterone receptors and HER2/neu were all negative in the tumor cells. Synaptophysin and chromogranin A were diffusely positive in the tumor cells. Cytokeratin 8 was only positive in a few tumor cells. The labeling indices... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2283682 Wed, 18 Mar 2009 06:49:28 +0100 2283682 http://www.medworm.com/index.php?rid=2064818&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp844k338u76l872j%2F We report an unusual case of adenomyoepithelioma associated with invasive ductal carcinoma here. Histologically, the lesion consisted of two separate tumors. One nodule corresponded to invasive ductal carcinoma consisting of tubular and trabecular arrangements of columnar or cuboidal neoplastic cells. The other tumor corresponded to adenomyoepithelioma consisting of an inner layer of neoplastic cells with basophilic cytoplasm and the outer layer of neoplastic cells with clear cytoplasm. Immunohistochemically, some myofibroblasts were observed in the stroma of both adenomyoepithelioma and invasive ductal carcinoma, but no CD34-positive stromal cells were seen in the stroma of either lesion. The stromal reaction of adenomyoepithelioma resembles that of intraductal papilloma in the prev... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064818 Wed, 24 Dec 2008 06:52:02 +0100 2064818 http://www.medworm.com/index.php?rid=2064821&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F68247233657v8655%2F Abstract  Stromal cells in the lamina propria of the human oviduct mucosa are unique cells that can differentiate into decidual cells during ectopic pregnancy in the oviduct. The nature of stromal cells is still unknown. In the present study, we investigated human oviductal stromal cells with transmission electron microscopy and immunohistochemistry and revealed that they had ultrastructural features similar to myofibroblasts and expressed alpha-smooth muscle actin, a marker used to identify myofibroblasts. Primary cilia were also one of the characteristic profiles of the stromal cells. These findings showed that the connective tissue-stromal cells in the human oviduct mucosa are myofibroblasts. They are considered to play an important role in the transport of oocytes by br... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064821 Wed, 24 Dec 2008 06:52:01 +0100 2064821 http://www.medworm.com/index.php?rid=2064820&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn1q86257r7372521%2F In conclusion, we suggest that the combination study of imprint cytology and FISH of chromosomes 10 and 21 as well as routine histology may contribute to the accurate diagnosis of chromophobe RCC. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0406-yAuthors Naoto Kuroda, Kochi Red Cross Hospital Departments of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanKazunobu Katto, Kochi Red Cross Hospital Department of Urology Kochi JapanTadanori Yamaguchi, Ayabe City Hospital Department of Pathology Kyoto JapanTakako Kawada, Maizuru Kyosai Hospital Department of Pathology Kyoto JapanYoshiaki Imamura, University of Fukui Hospital Division of Surgical Pathology Fukui JapanOndrej Hes, Charles University Hospital Department of Pathology Plzen Czech Rep... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064820 Wed, 24 Dec 2008 06:52:01 +0100 2064820 http://www.medworm.com/index.php?rid=2064819&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F77213732t20u7720%2F Abstract  An autopsy of a 44-year-old Japanese woman with mitochondrial cytopathy confirmed the presence of chronic progressive external ophthalmoplegia (CPEO). Immunohistochemistry using antimitochondrial antibody was performed to observe the ultrastructure of the skeletal muscle and renal tissues. The patient was born of consanguineous parents, developed normally, and was of average intelligence. At 22 years of age, the patient noticed hearing loss, and subsequently, over time, developed a progressive generalized muscle weakness, which included limitation of eye movement and ptosis. At age 41, a muscle biopsy was performed using the modified Gomori trichrome method and demonstrated the presence of ragged red fibers. After the evaluation of her results in conjunction with ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064819 Wed, 24 Dec 2008 06:52:01 +0100 2064819 http://www.medworm.com/index.php?rid=3099953&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd9344jq54072v2xv%2F Abstract  Activating point mutations of the mouse Kras2 oncogene or its human homologue, KRAS, are critical for lung adenocarcinoma genesis, independent of the species. Significantly, in the mouse, several polymorphic Kras2 alleles have been identified, which cosegregate with genetic susceptibility to chemical induction of lung tumors. Moreover, a major lung tumor susceptibility locus, the Pas1 (Pulmonary adenoma susceptibility 1), was found to colocalize with Kras2 on distal chromosome 6 on linkage analysis. The Kras2 may thus be involved in both cellular transformation and genetic control of tumor susceptibility. In this review, the focus is on current knowledge regarding the relationship between Kras2 and experimental mouse lung carcinogenesis, especially from the aspect of... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3099953 Wed, 24 Dec 2008 06:51:56 +0100 3099953 http://www.medworm.com/index.php?rid=2064823&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd9344jq54072v2xv%2F Abstract  Activating point mutations of the mouse Kras2 oncogene or its human homologue, KRAS, are critical for lung adenocarcinoma genesis, independent of the species. Significantly, in the mouse, several polymorphic Kras2 alleles have been identified, which cosegregate with genetic susceptibility to chemical induction of lung tumors. Moreover, a major lung tumor susceptibility locus, the Pas1 (Pulmonary adenoma susceptibility 1), was found to colocalize with Kras2 on distal chromosome 6 on linkage analysis. The Kras2 may thus be involved in both cellular transformation and genetic control of tumor susceptibility. In this review, the focus is on current knowledge regarding the relationship between Kras2 and experimental mouse lung carcinogenesis, especially from the aspect of... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064823 Wed, 24 Dec 2008 06:51:56 +0100 2064823 http://www.medworm.com/index.php?rid=2064822&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm7555h65v11286q2%2F In conclusion, NaN3 induces necrotic cell death in SCC131. This experimental model may be used in the study of necrotic cell death. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0414-yAuthors Eiju Sato, Fukushima Medical University Department of Dentistry & Oral Surgery Fukushima JapanToshimitsu Suzuki, Fukushima Medical University Department of Pathology Fukushima JapanNobuo Hoshi, Fukushima Medical University Department of Pathology Fukushima JapanTakashi Sugino, Fukushima Medical University Department of Pathology Fukushima JapanHiroshi Hasegawa, Fukushima Medical University Department of Dentistry & Oral Surgery Fukushima Japan Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 41 Journal I... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064822 Wed, 24 Dec 2008 06:51:56 +0100 2064822 http://www.medworm.com/index.php?rid=2064826&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu3h4l162m0476475%2F Abstract  A few studies of the morphogenesis of human herpesvirus (HHV) 6 type A and B (HHV-6A, -6B) have been performed using neurogenic, lymphoid, or epithelial cells. When human MT-4 T-lymphotropic virus type I (HTLV-I)-producing lymphoid cells were coinfected with HHV-6B in vitro, viral-specific proteins were clearly detected. We therefore attempted to detect virus particles at the ultrastructural level, focusing on the morphogenesis of such particles. Ultrastructurally, HHV-6B virus particles could be observed in the nuclei, cytoplasm, and extracellular spaces of MT-4 cells, in addition to extracellular HTLV-I particles of C type. In the nuclei, dense-cored or doughnut-shaped viral capsids were found, as well as peculiar tubular rods. When budding to perinuclear spaces... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064826 Wed, 24 Dec 2008 06:51:55 +0100 2064826 http://www.medworm.com/index.php?rid=2064825&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fcgqv7r06520hju47%2F Abstract  The human oviduct is lined with a simple columnar epithelium composed of ciliated cells and secretory cells. Primary cilia or solitary cilia usually extend from the apical surface of the secretory cells. The axoneme of the primary cilia is composed of nine peripheral microtubule doublets (9 + 0 pattern) that lack dynein arms and nexin links. Displacement of peripheral doublets to the central region, which is suggested to be attributable to the lack of nexin links, is one of the distinctive features of oviductal primary cilia. The basal body that extends the primary cilium connects to its paired centriole by the striated connector. The basal body is associated with the accessory structures, such as alar sheets, basal feet, and striated rootlets. Several basal feet ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064825 Wed, 24 Dec 2008 06:51:55 +0100 2064825 http://www.medworm.com/index.php?rid=2064824&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv3vk24u0447775n3%2F This article describes the achievements to date in production of biological teeth, mostly from our laboratory. In particular, we describe the potential to produce teeth from embryonic and postnatal tooth buds. Content Type Journal ArticleCategory ReviewDOI 10.1007/s00795-008-0416-9Authors Masaki J. Honda, Nihon University School of Dentistry Department of Anatomy 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310 JapanHanson Fong, University of Washington Department of Materials Science & Engineering Seattle WA USAShinji Iwatsuki, University of Tokyo Tooth Regeneration, Division of Stem Cell Engineering, Institute of Medical Science Tokyo JapanYoshinori Sumita, University of Tokyo Tooth Regeneration, Division of Stem Cell Engineering, Institute of Medical Science Tokyo JapanMehmet Sa... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064824 Wed, 24 Dec 2008 06:51:55 +0100 2064824 http://www.medworm.com/index.php?rid=2064827&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb1583710016k3004%2F Content Type Journal ArticleCategory ErratumDOI 10.1007/s00795-008-0426-7Authors Fumihiko Kaneko, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanHiroaki Yokomori, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanAya Sato, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanHajime Takeuchi, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanKumiko Tahara, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanYasunobu Sekiguchi, Saitama Medical University D... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=2064827 Wed, 24 Dec 2008 06:51:54 +0100 2064827 http://www.medworm.com/index.php?rid=3096103&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh706450h47j6710j%2F Abstract  Fourteen-membered-ring macrolides have an antiinflammatory effect, in addition to their antibacterial effect, and are widely used at low dosages for long-term therapy for chronic inflammatory disease such as diffuse pan-bronchiolitis and chronic sinusitis. A macrolide-resistant coagulase-negative staphylococcal strain was obtained from the maxillary sinus of a patient with chronic sinusitis, who failed long-term macrolide therapy. The isolated strain was characterized as Staphylococcus capitis and had an MIC for erythromycin greater than 128 μg/ml. Morphological observation demonstrated that this macrolide-resistant S. capitis strain had a thicker cell wall than macrolide-sensitive S. capitis strains. Moreover, the strain was not carrying any other than the four ge... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3096103 Sat, 20 Sep 2008 10:05:02 +0100 3096103 http://www.medworm.com/index.php?rid=1814892&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8105r71384574454%2F In this study, we examined the expression of RALDH1 in diethylstilbestrol-induced prolactinoma of LEXF RI rats. Quantitative analysis of mRNA levels by real-time PCR demonstrated drastic reduction of RALDH1 expression in the prolactinoma. We have also detected both mRNA expression and production by in situ hybridization and immunohistochemistry. Both mRNA-expressing cells and immunopositive cells remarkably decreased after 4 weeks of treatment with diethylstilbestrol. Fluorescence double immunohistochemistry of RALDH1 and prolactin revealed that prolactin-immunopositive cells do not colocalize with RALDH1 in the prolactinoma. These results suggest that the reduction of local RA generation relates to cell proliferation and tumorigenesis of lactotrophs. Content Type Journal ArticleC... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814892 Sat, 20 Sep 2008 10:05:02 +0100 1814892 http://www.medworm.com/index.php?rid=1814891&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq420512521808155%2F Abstract  Brush cells (BCs) are relatively rare cells that are sparsely distributed throughout the mammalian digestive and respiratory systems. BCs have been identified in the rodent large intestine, but these cells have not been characterized by immunocytochemistry or electron microscopy. We previously demonstrated that rat bile duct BCs had strong immunoreactivity for six proteins that function in HCO3− secretion and thus assumed that BCs secrete NaHCO3. It is well known that the gastrointestinal (GI) tract secretes NaHCO3, but it is not known whether BCs of the GI tract also express proteins related to HCO3− secretion. Thus, in the present study, using double immunostaining for cytokeratin 18, a specific marker for BCs, we investigated protein expression in BCs from th... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814891 Sat, 20 Sep 2008 10:05:02 +0100 1814891 http://www.medworm.com/index.php?rid=1814890&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fqmx7115370502132%2F Abstract  Raloxifene is a nonsteroidal benzothiophene that has also been classified as a selective estrogen receptor modulator (SERM) on the basis of studies in which it produced both estrogen-agonistic effects on bone and lipid metabolism and estrogen-antagonistic effects on uterine endometrium and breast tissue. We investigated apoptotic cell death and the apoptotic pathway in human endometrial carcinoma cells (Ishikawa cells) expressing estrogen receptor treated with raloxifene. Cell viability was significantly decreased in Ishikawa cells treated with raloxifene at 20 μM and higher levels. Raloxifene at 20 μM induced 54% inhibition of cell viability after 48 h treatment. Apoptotic parameters were analyzed for determination of apoptotic pathway in Ishikawa cells treated... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814890 Sat, 20 Sep 2008 10:05:02 +0100 1814890 http://www.medworm.com/index.php?rid=1814889&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh706450h47j6710j%2F Abstract  Fourteen-membered-ring macrolides have an antiinflammatory effect, in addition to their antibacterial effect, and are widely used at low dosages for long-term therapy for chronic inflammatory disease such as diffuse pan-bronchiolitis and chronic sinusitis. A macrolide-resistant coagulase-negative staphylococcal strain was obtained from the maxillary sinus of a patient with chronic sinusitis, who failed long-term macrolide therapy. The isolated strain was characterized as Staphylococcus capitis and had an MIC for erythromycin greater than 128 μg/ml. Morphological observation demonstrated that this macrolide-resistant S. capitis strain had a thicker cell wall than macrolide-sensitive S. capitis strains. Moreover, the strain was not carrying any other than the four ge... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814889 Sat, 20 Sep 2008 10:05:02 +0100 1814889 http://www.medworm.com/index.php?rid=3096104&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F70170x6515421h44%2F Abstract  Dark-skinned people are known to be more tolerant of ototraumatic noise than are light-skinned people, and pigmented animals are more tolerant of ototraumatic noise and aminoglycoside ototoxicity than are albino animals. Such tolerance may be dependent on the local ability of detoxification and antioxidant enzymes, including glutathione S-transferase (GST) and glutathione peroxidase (GSPx). In the present study, we examined the difference in GST/GSPx expression in the lateral wall of the cochlea between pigmented and albino guinea pigs. Eight-week-old male pigmented and albino guinea pigs were killed by transcardiac perfusion with 2% paraformaldehyde. The cochlear ducts were isolated, further fixed with 4% paraformaldehyde, decalcified, and then embedded in paraffi... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=3096104 Sat, 20 Sep 2008 10:05:01 +0100 3096104 http://www.medworm.com/index.php?rid=1814894&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F70170x6515421h44%2F Abstract  Dark-skinned people are known to be more tolerant of ototraumatic noise than are light-skinned people, and pigmented animals are more tolerant of ototraumatic noise and aminoglycoside ototoxicity than are albino animals. Such tolerance may be dependent on the local ability of detoxification and antioxidant enzymes, including glutathione S-transferase (GST) and glutathione peroxidase (GSPx). In the present study, we examined the difference in GST/GSPx expression in the lateral wall of the cochlea between pigmented and albino guinea pigs. Eight-week-old male pigmented and albino guinea pigs were killed by transcardiac perfusion with 2% paraformaldehyde. The cochlear ducts were isolated, further fixed with 4% paraformaldehyde, decalcified, and then embedded in paraffi... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814894 Sat, 20 Sep 2008 10:05:01 +0100 1814894 http://www.medworm.com/index.php?rid=1814893&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp7ph618752g67jl5%2F Abstract  In contrast to invasive ductal carcinoma, invasive lobular carcinoma (ILC) of the breast is characterized by multiple ipsilateral occurrences and a higher incidence in the contralateral breast. It is therefore necessary to examine thoroughly whether there is any other carcinoma present before any breast-conserving surgery is carried out. We cytologically, histologically, and ultrastructurally investigated ILC and pure scirrhous carcinoma (PSC), a subtype of invasive ductal carcinoma, to establish cytological diagnostic criteria for the differential diagnosis of these two types of carcinoma that have high histological similarity. Cytologically, ILC cells showed linear or isolated cell arrangements and had small nuclei with round homogeneously distributed fine granu... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814893 Sat, 20 Sep 2008 10:05:01 +0100 1814893 http://www.medworm.com/index.php?rid=1814898&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm63057081w016342%2F We report a rare case of coexisting pulmonary adenocarcinoma and Langerhans’ cell histiocytosis (LCH) in a 78-year-old woman who did not smoke. During follow-up of diabetes mellitus, she had complained of chest pain and was found to have a nodular lesion in S9 of the left lower lobe, which was resected surgically. No abnormal laboratory findings were obtained. Before surgical resection, needle biopsy specimens confirmed the existence of adenocarcinoma. The resected tumor in the left lower lobe was 3.0 × 1.8 × 3.0 cm, and histologically both acinar and bronchioloalveolar cell subtypes of adenocarcinoma were found in cancer foci. In addition to pulmonary adenocarcinoma, Langerhans’ cell proliferation associated with marked eosinophil infiltration was incidentally found in a small... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814898 Sat, 20 Sep 2008 10:05:00 +0100 1814898 http://www.medworm.com/index.php?rid=1814897&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw7626557142p80x7%2F We report a case of primary hepatic non-Hodgkin’s lymphoma in a 67-year-old man with chronic hepatitis C. Laboratory data revealed slightly elevated liver function parameters and positive for hepatitis C virus (HCV) antibody. Abdominal ultrasonography showed hypoechoic lesions approximately 5 mm in diameter in the whole liver. Magnetic resonance imaging showed that the tumors were isointense in relationship to the liver on T1-weighted images but were slightly hyperintense on T2-weighted images. Under a clinical diagnosis of liver tumor, liver biopsy was performed. Histological examination confirmed a diagnosis of non-Hodgkin’s diffuse large B-cell lymphoma, and the immunophenotype was identified to be the germinal cell type. Content Type Journal ArticleCategory Original PaperDO... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814897 Sat, 20 Sep 2008 10:05:00 +0100 1814897 http://www.medworm.com/index.php?rid=1814896&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F623655413786550m%2F We report here an additional case of this type of tumor that arose from the left ovary in a 76-year-old woman. By light microscopy, the tumor was composed of small round and oval cells with cytoplasmic vacuolization and a typical signet-ring appearance, focally admixed with fibromatous tissue. Special staining revealed that the vacuoles of the tumor cells contained no lipid, mucoprotein, or glycogen. Interestingly, variously sized hyaline globule-like structures positive for periodic acid-Schiff (PAS) reaction with and without diastase digestion were present in the cytoplasm of tumor cells. Immunohistochemically, the tumor cells variously expressed several markers, including CD56, inhibin-α, α-smooth muscle actin (α-SMA), and progesterone receptor (PR), which have been identified ... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814896 Sat, 20 Sep 2008 10:05:00 +0100 1814896 http://www.medworm.com/index.php?rid=1814895&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn18554774h24mm50%2F Abstract  To elucidate more precisely the biological characteristics of neuroblastomas, we examined four human neuroblastomas heterotransplanted into athymic nude mice NB-39 (undifferentiated type), NB-45 (poorly differentiated type with undifferentiated component), NB-52 (poorly differentiated type), and NB-726 (differentiating type) by electron microscopy, immunohistochemistry, and radioimmunoassay for the peptides in tumors. Ultrastructurally, NB-45, NB-52, and NB-726 contained more numerous and variously sized neurosecretory granules than did NB-39. Immunohistochemistry revealed neurofilament proteins, tyrosine hydroxylase, neuropeptide Y (NPY), and chromogranin A-positive cells in the four tumors in the following order of frequency: NB-726, NB-45, NB-52, and NB-39. NB-... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814895 Sat, 20 Sep 2008 10:05:00 +0100 1814895 http://www.medworm.com/index.php?rid=1814899&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd1368121050t162x%2F Abstract  Elastofibroma is a rare neoplasm. In this article, we report our study of the pathogenesis of fibrosis in elastofibroma. Three tumors obtained from two patients were selected. One patient was a 57-year-old Japanese woman who had a bilateral tumor, and the other patient was a 83-year-old Japanese man. All tumors occurred in the infrascapular region. Macroscopically, the cut surface of all tumors showed a poorly defined and whitish mass with yellowish foci. Microscopically, the tumor consisted of collagen fiber bundles, abnormal elastic fibers, and spindle cells suggestive of fibroblasts. Elastica-van Gieson and Masson-trichrome stain identified abnormal elastic fibers and abundant collagen fibers, respectively, in elastofibroma. Immunohistochemically, fibroblasts w... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1814899 Sat, 20 Sep 2008 10:04:59 +0100 1814899 http://www.medworm.com/index.php?rid=1563267&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk2487066h2532465%2F Content Type Journal ArticleCategory Special Series: Molecular morphological approach for pathogenesis of endocrine diseasesDOI 10.1007/s00795-008-0398-7 Journal Medical Molecular MorphologyOnline ISSN 1860-1499Print ISSN 1860-1480 Journal Volume Volume 41 Journal Issue Volume 41, Number 2 / June, 2008 (Source: Medical Molecular Morphology) Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1563267 Tue, 01 Jul 2008 06:06:38 +0100 1563267 http://www.medworm.com/index.php?rid=1563268&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb08070x3q804m291%2F In conclusion, we suggest that D2-40 may be an available marker of GISTs, but the expression of D2-40 seems to be not associated with the degree of risk of aggressive behavior. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-007-0393-4Authors Naoto Kuroda, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanNobuyuki Tanida, Kochi Red Cross Hospital Department of Surgery Kochi JapanKazuhisa Oonishi, Kochi Red Cross Hospital Department of Surgery Kochi JapanKaori Inoue, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanMasahiko Ohara, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanKeiko Mizuno, Kochi Red Cross... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1563268 Tue, 01 Jul 2008 06:06:37 +0100 1563268 http://www.medworm.com/index.php?rid=1563271&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft5127427wl8m8397%2F Abstract  The mechanisms explaining the clinical effects of direct maxacalcitol (OCT) injection into the hyperplastic parathyroid gland (PTG) in uremic patients with advanced secondary hyperparathyroidism (SHPT) were investigated by molecular and morphological examination. PTG of uremia-induced SHPT model rats were treated by a direct injection of OCT (DI-OCT) or vehicle (DI-vehicle). The changes in serum intact parathyroid hormone (intact-PTH) level, vitamin D and Ca-sensing receptor (VDR and CaSR, respectively) expression levels in PTG, and the calcium (Ca)-PTH response curve were examined; the induction of apoptosis in parathyroid cells (PTC) was also analyzed by the TUNEL method, DNA electrophoresis, and electron microscopic examination. Serum intact-PTH level following... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1563271 Tue, 01 Jul 2008 06:06:36 +0100 1563271 http://www.medworm.com/index.php?rid=1563270&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjwt7151876l5gv2g%2F In conclusion, albumin will be filtrated through the decreased numbers of slit diaphragms; however, albumin can be also taken up in the podocyte, the mesangium, and the glomerular endothelium, suggesting that there might be other routes of glomerular albumin clearance in nephrotic syndrome. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0397-8Authors Akihiro Tojo, University of Tokyo Division of Nephrology and Endocrinology 7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655 JapanMaristela Lika Onozato, University of Tokyo Division of Nephrology and Endocrinology 7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655 JapanChagriya Kitiyakara, Mahidol University Renal Division, Ramathibodi Hospital Bangkok ThailandSatoshi Kinugasa, University of Tokyo Division of Nephrology and Endocrino... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1563270 Tue, 01 Jul 2008 06:06:36 +0100 1563270 http://www.medworm.com/index.php?rid=1563269&cid=s_33327_67_f&fid=33327&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F305342736t541204%2F Abstract  Between 2004 and 2007, we examined a total of 70 waterfowl. Forty of 51 (78.4%) mute swans (Cygnus olor) had amyloidosis. Amyloid deposits were detected in the spleen of 39 of 49 birds (79.6%), liver of 37 of 47 birds (78.7%), intestine of 38 of 50 birds (76.0%), pancreas of 30 of 42 birds (71.4%), kidney of 32 of 47 birds (68.1%), thyroid gland of 20 of 30 birds (66.7%), heart of 26 of 49 birds (53.1%), and lung of 5 of 45 birds (11.1%). In some birds, there was a globular pattern of amyloid deposition or infiltration of foreign-body giant cells around amyloid nodules in the spleen. Immunostaining with anti-AA antibody and Western blotting revealed that all were cases of AA amyloidosis. In sections treated with potassium permanganate, which removes Congo red stain... Medical Molecular Morphology journals http://www.medworm.com/rss/comments.php?id=1563269 Tue, 01 Jul 2008 06:06:36 +0100 1563269