MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Microbial Cell Factories' source. Mon, 06 Feb 2012 21:32:18 +0100 http://www.medworm.com/index.php?rid=5664674&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F20 Conclusions: The export mechanism of autotransporters is not well understood but accumulating evidence suggest a critical role for the native effector or alpha domain in facilitating its own export via interactions with the translocation or beta domain. This is the first report directly comparing expression of heterologous proteins fused to the full length IcsA autotransporter and fusion to the beta domain alone. Protein expression and surface presentation of the fusion proteins were dramatically improved when fused to IcsA rather than IcsAbeta. Future studies involved in designing autotransporters as cell surface display vehicles would benefit from including the native alpha domain. This work also provides further evidence for a key interaction between the autotransporter alpha and beta d... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5664674 Tue, 07 Feb 2012 02:08:30 +0100 5664674 http://www.medworm.com/index.php?rid=5664675&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F19 Conclusions: The transcription strength of the MCPtacs promoter cluster can be greatly improved by increasing the tandem repeats number of the core-tac-promoter. By integrating the desired gene together with the MCPtacs promoter cluster into the chromosome of E. coli, we can achieve high and stale overexpression with only a small size. This strategy has an application potential in many fields and can be extended to other bacteria. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5664675 Mon, 06 Feb 2012 05:00:00 +0100 5664675 http://www.medworm.com/index.php?rid=5657256&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F16 Conclusions: AMDs have been studied for years especially with regard to interactions between bacteria and the inorganic compartment hosting them. To date, no study reported the role of microorganisms in the recycling of the organic matter. The present work suggests that the strain Q8 might play an important role in the community by recycling the scarce organic matter (cellulose, hemicellulose, starch...), especially when the conditions change. Furthermore, function-based screening of a Q8 DNA library allowed to assign an amylolytic function to a gene previously unknown. AMDs could be considered as a reservoir of genes with potential biotechnological properties. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5657256 Fri, 03 Feb 2012 05:00:00 +0100 5657256 http://www.medworm.com/index.php?rid=5657255&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F17 Conclusions: This study identifies several mechanisms regulating the synthesis of curdlan, an EPS with numerous applications. These mechanisms are potential metabolic engineering targets for improving the industrial production of curdlan from Agrobacterium sp. ATCC 31749. Furthermore, many of the genes identified in this study are highly conserved across microbial genomes, and we propose that the molecular elements identified in this study may serve as universal regulators of microbial EPS synthesis. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5657255 Fri, 03 Feb 2012 05:00:00 +0100 5657255 http://www.medworm.com/index.php?rid=5657254&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F18 Conclusion: In mixed fermentation, S. bombicola immobilized cells greatly affected the fermentation behavior of S. cerevisiae and the analytical composition of wine. The influence of S. bombicola on S. cerevisiae was not limited to a simple additive contribution. Indeed, its presence caused metabolic modifications during S. cerevisiae fermentation causing variation in the gene expression and enzymatic activity of alcohol deydrogenase and pyruvate decarboxilase. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5657254 Fri, 03 Feb 2012 05:00:00 +0100 5657254 http://www.medworm.com/index.php?rid=5657257&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F15 Conclusions: In this study we have provided the first evidence of protein O-glycosylation in the probiotic L rhamnosus GG. The major secreted protein Msp1 is glycosylated with a ConA reactive glycan at the serine residues at 106 and 107. Glycosylation is not required for the peptidoglycan hydrolase activity of Msp1 nor for Akt activation capacity in epithelial cells, but appears to be important for its stability and protection against proteases. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5657257 Wed, 01 Feb 2012 05:00:00 +0100 5657257 http://www.medworm.com/index.php?rid=5657258&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F14 Conclusions: The one-step method enables producing epPCR libraries of as high complexity and quality as does the regular, two-step, protocol for half the amount of work. In addition, it contributes to preserve the original complexity of the epPCR product. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5657258 Mon, 30 Jan 2012 05:00:00 +0100 5657258 http://www.medworm.com/index.php?rid=5636022&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F13 Conclusion: Bacterial engineered glycoproteins show promising applications for the development on an array of diagnostics and immunoprotective opportunities in the future. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5636022 Wed, 25 Jan 2012 05:00:00 +0100 5636022 http://www.medworm.com/index.php?rid=5625307&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F12 Conclusions: This work demonstrated the attitude of L. muscarium CCFEE 5003 to grow in bench-top bioreactor; outlined the strong influence of aeration and agitation on its growth and enzyme production and identified the optimal conditions for possible production at the industrial level. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5625307 Mon, 23 Jan 2012 05:00:00 +0100 5625307 http://www.medworm.com/index.php?rid=5617570&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F11 Conclusions: By eliminating stress-inducible error-prone DNA-polymerases, the genome of the mobile genetic element-free E. coli strain MDS42 was further stabilized. The resulting strain represents an improved host in various synthetic and molecular biological applications, allowing more stable production of growth-inhibiting biomolecules. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5617570 Fri, 20 Jan 2012 05:00:00 +0100 5617570 http://www.medworm.com/index.php?rid=5604550&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F10 Conclusions: This study shows that the surfactant-like peptides L6KD and it derivatives can act as a pull-down handler for converting soluble proteins into active aggregates, much like 18A and ELK16. These peptide-mediated protein aggregations might have important implications for protein aggregation in vivo, and can be explored for production of functional biopolymers with detergent or other interfacial activities. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5604550 Wed, 18 Jan 2012 05:00:00 +0100 5604550 http://www.medworm.com/index.php?rid=5604551&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F9 Conclusion: We observed good agreement of the physiological data obtained in the developed MTP platform with bench-scale. Hence, the described MTP-based screening platform has a high potential for investigation of secondary metabolite biosynthesis in Streptomycetes and other filamentous bacteria and the use may significantly reduce the workload and costs. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5604551 Tue, 17 Jan 2012 05:00:00 +0100 5604551 http://www.medworm.com/index.php?rid=5594078&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F8 Conclusions: In the present study, T. fusca cutinase was successfully secreted to the culture media by alpha-hemolysin secretion system. This is the first report of cutinase being efficiently secreted by this pathway. Due to the limited cases of successful expression of industrial enzyme by E. coli alpha-hemolysin secretion system, our study further explored the utilization of this pathway in industrial enzymes. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5594078 Thu, 12 Jan 2012 05:00:00 +0100 5594078 http://www.medworm.com/index.php?rid=5594079&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F7 Conclusions: Expression engineering of C. boidinii FDH is an important strategy to optimize E. coli whole-cell reductase catalysts that employ intracellular formate oxidation for regeneration of NADH. Increased FDH-activity was reflected by higher reduction yields of D-xylose and o-chloroacetophenone conversions provided that mass transfer limitations were overcome. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5594079 Wed, 11 Jan 2012 05:00:00 +0100 5594079 http://www.medworm.com/index.php?rid=5594081&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F5 Conclusions: In summary, CPPs cause a reprogramming of cellular metabolism leading to massive secretion of extracellular proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5594081 Tue, 10 Jan 2012 05:00:00 +0100 5594081 http://www.medworm.com/index.php?rid=5594080&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F6 Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, the need to obtain large quantities of the recombinant protein for research purposes has been essential. Currently, production of recombinant PrP is achieved by refolding protocols. Here, we show that the co-expression of two different PrP with the human Quiescin Sulfhydryl OXidase (QSOX), a human chaperone with thiol/disulfide oxidase activity, in the cytoplasm of E. coli produces soluble recombinant PrP. The structural integrity of the soluble PrP has been confirmed by nuclear magnetic resonance spectroscopy, demonstrating that properly folded PrP can be easily expresse... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5594080 Tue, 10 Jan 2012 05:00:00 +0100 5594080 http://www.medworm.com/index.php?rid=5577125&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F4 Conclusions: The identified proteins suggest that the fermentative capacity detriment observed under industrial conditions in T73 wine commercial strain results from the oxidative carbonylation of specific glycolytic and fermentation enzymes. Indeed, increased thioredoxin levels enhance the performance of key fermentation enzymes such as Adh1p, which consequently increases fermentative capacity. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5577125 Mon, 09 Jan 2012 05:00:00 +0100 5577125 http://www.medworm.com/index.php?rid=5569750&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F3 Conclusion: Coupling gene expression to cell density using cell-to-cell communication provides a promising approach for recombinant protein production. The system allows the control of expression of the target recombinant gene independently from external inducers or drastic changes in metabolic conditions and enabling tight regulation of expression . (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5569750 Thu, 05 Jan 2012 05:00:00 +0100 5569750 http://www.medworm.com/index.php?rid=5569751&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F2 Conclusion: Here, we report for the first time the potential application of proteorhodopsin for the production of biohydrogen, a promising alternative fuel. We showed that H2 production was enhanced by the co-expression of proteorhodopsin and [NiFe]-hydrogenase in recombinant E. coli BL21(DE3) in a light intensity-dependent manner. These results demonstrate that E. coli can be applied as light-powered cell factories for biohydrogen production by introducing proteorhodopsin. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5569751 Wed, 04 Jan 2012 05:00:00 +0100 5569751 http://www.medworm.com/index.php?rid=5569752&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F11%2F1%2F1 Conclusions: Following evolution, both Candida and Saccharomyces cells were able to proliferate up to 2.5 g * L-1 CuSO4 and to accumulate high amounts of intracellular copper. The comparison of yeasts differing in their robustness, allowed highlighting physiological and molecular determinants of natural and acquired copper tolerance. We observed that different mechanisms contribute to confer metal tolerance: the control of copper uptake, changes in the levels of enzymes involved in oxidative stress response and changes in the copper-binding proteome. However, copper elicits different physiological and molecular reactions in yeasts with different backgrounds. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5569752 Tue, 03 Jan 2012 05:00:00 +0100 5569752 http://www.medworm.com/index.php?rid=5550307&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F113 Conclusions: We demonstrate that CDH offers an attractive tool for saccharification process enhancement due to gluconic acid production from raw lignocellulosic material. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5550307 Wed, 28 Dec 2011 05:00:00 +0100 5550307 http://www.medworm.com/index.php?rid=5550306&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F114 Conclusions: The optimization of fermentation variables using conventional, statistical approaches and aeration/agitation at fermentor level resulted in ~13.5 folds increase (882 Uml-1) in protease production compared to un-optimized conditions (65 Uml-1). This is the highest level of thermoalkaline protease reported so far by any psychrotrophic bacterium. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5550306 Wed, 28 Dec 2011 05:00:00 +0100 5550306 http://www.medworm.com/index.php?rid=5534297&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F112 Conclusions: Our results indicated that the pro-peptide can assist correct folding of the TGase inter-molecularly in E. coli, and expression of pro-peptide prior to that of TGase was essential for the production of active TGase. The co-expression strategy based on optimizing the order of gene expression could be useful for the expression of other functional proteins that are synthesized as a precursor. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5534297 Fri, 23 Dec 2011 05:00:00 +0100 5534297 http://www.medworm.com/index.php?rid=5534298&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F111 Conclusion: C. saccharolyticus is able to grow and produce hydrogen at high PH2, hence eliminating the need of gas sparging in its cultures. Under this condition, it has a unique ability to fine tune its metabolism by maintaining the glycolytic flux through regulating GAPDH activity and redistribution of pyruvate flux. Concerning the later, xylose-rich feedstock should be preferred over the sucrose-rich one for better H2 yield. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5534298 Wed, 21 Dec 2011 05:00:00 +0100 5534298 http://www.medworm.com/index.php?rid=5520962&cid=s_34082_77_f&fid=34082&url=%24%7Bitem.link%7D Conclusions: The shear and/or oxygenation parameters determine the bacterial morphology, the productivity, and the O-mannosylation of rAPA in S. lividans. As demonstrated here, culture conditions have to be carefully controlled in order to obtain recombinant O-glycosylated proteins with similar "quality" in bacteria, particularly, if the protein activity depends on the glycosylation pattern. Furthermore, it will be an interesting exercise to determine the effect of shear and oxygen in shake flasks, to obtain evidences that may be useful in scaling-up these processes to bioreactors. Another approach will be using lab-scale bioreactors under well-controlled conditions, and study the impact those on rAPA productivity and quality. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5520962 Tue, 20 Dec 2011 05:00:00 +0100 5520962 http://www.medworm.com/index.php?rid=5513027&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F109 Conclusions: Recombinant scFv-Fc proteins were expressed in yeast with low efficiency. New approach to display the scFv-Fc molecules on the surface of pseudotype VLPs was successful and allowed generation of multivalent scFv-Fc proteins with high VLY-neutralizing potency. Our study demonstrated for the first time that large recombinant antibody molecule fused with hamster polyomavirus VP2 protein and co-expressed with VP1 protein in the form of pseudotype VLPs was properly folded and exhibited strong antigen-binding activity. The current study broadens the potential of recombinant VLPs as a highly efficient carrier for functionally active complex proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5513027 Thu, 15 Dec 2011 05:00:00 +0100 5513027 http://www.medworm.com/index.php?rid=5501820&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F108 Conclusions: A novel bgaL gene was isolated from Paracoccus sp. 32d encoded a novel cold-active beta-D-galactosidase. An E. coli expression system has enabled efficient production of soluble form of BgaL Paracoccus sp. 32d. The amino acid sequence analysis of the BgaL enzyme revealed notable differences in comparison to the result of the amino acid sequences analysis of well-characterized cold-active beta-D-galactosidases belonging to Glycoside Hydrolase Family 2. Finally, the enzymatic properties of Paracoccus sp. 32d beta-D-galactosidase shows its potential for being applied to development of a new industrial biocatalyst for efficient lactose hydrolysis in milk. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5501820 Tue, 13 Dec 2011 05:00:00 +0100 5501820 http://www.medworm.com/index.php?rid=5501822&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F106 Conclusions: The heterologous expression of active GDH was greatly favoured in the eukaryotic host. The efficient expression in P. pastoris facilitates the production of genetically engineered GDH variants for electrochemical-, physiological- and structural studies. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5501822 Mon, 12 Dec 2011 05:00:00 +0100 5501822 http://www.medworm.com/index.php?rid=5501821&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F107 This report describes the combined use of an enzyme-based glucose release system (EnBase(R)) and high-aeration shake flask (Ultra Yield FlaskTM). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5501821 Mon, 12 Dec 2011 05:00:00 +0100 5501821 http://www.medworm.com/index.php?rid=5493038&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F105 Conclusions: Our system, which combines a downregulated version of the tightly repressed PBAD promoter with a TF-deficient host may prove a valuable alternative to T7-based expression for the production of membrane proteins that have so far remained elusive targets. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5493038 Fri, 09 Dec 2011 05:00:00 +0100 5493038 http://www.medworm.com/index.php?rid=5483946&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F104 Conclusions: Our study underlines the dynamic character of adaptive responses of cells towards a change in their nutrient access. The gradual decrease of the phosphate supply resulted in a step-wise modulated phenotypic response, thereby alternating the specific productivity and the secretory flux. Our work emphasizes the importance of reduced phosphate supply for improved secretory production of heterologous proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5483946 Wed, 07 Dec 2011 05:00:00 +0100 5483946 http://www.medworm.com/index.php?rid=5483947&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F103 Conclusions: The present study has revealed the specific features of the first characterized endo beta-1,4 glucanase from yeast, whose thermostability is promising for biotechnological applications related to the saccharification of lignocellulosic biomass such as consolidated bioprocessing. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5483947 Tue, 06 Dec 2011 05:00:00 +0100 5483947 http://www.medworm.com/index.php?rid=5483948&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F102 Conclusions: This study is a proof of concept for the possibility to engineer in a filamentous fungus a bacterial enzyme cascade, which is fully functional. Furthermore, it provides the basis for the development of a process for NeuNAc production as well as a general prospective design for production processes that use saprophytes as whole-cell catalysts. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5483948 Mon, 05 Dec 2011 05:00:00 +0100 5483948 http://www.medworm.com/index.php?rid=5465796&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F101 Histidine-rich peptides are commonly used in recombinant protein production as purification tags, allowing the one-step affinity separation of the His-tagged proteins from the extracellular media or cell extracts. Genetic engineering makes feasible the post-purification His-tag removal by inserting, between the tag and the main protein body, a target site for trans-acting proteases or a self-proteolytic peptide with regulatable activities. However, for technical ease, His tags are often not removed and the fusion proteins eventually used in this form. In this commentary, we revise the powerful biological properties of histidine-rich peptides as endosomolytic agents and as architectonic tags in nanoparticle formation, for which they are exploited in drug delivery and other nanomedical appli... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5465796 Fri, 02 Dec 2011 05:00:00 +0100 5465796 http://www.medworm.com/index.php?rid=5448025&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F100 Conclusions: The effect of several organic acids on the growth of different bacteria was accurately studied and perfectly characterized by a bivariate equation which combines the basis of dose-response theory with microbial growth kinetics (secondary model). The toxicity of carboxylic acids was lower with the increase of the molecular weight of these chemicals. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5448025 Fri, 25 Nov 2011 05:00:00 +0100 5448025 http://www.medworm.com/index.php?rid=5418596&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F99 Hyaluronic acid (HA) is a natural and linear polymer composed of repeating disaccharide units of beta-1, 3-N-acetyl glucosamine and beta-1, 4-glucuronic acid with a molecular weight up to 6 million Daltons. With excellent viscoelasticity, high moisture retention capacity, and high biocompatibility, HA finds a wide-range of applications in medicine, cosmetics, and nutraceuticals.Traditionally HA was extracted from rooster combs, and now it is mainly produced via streptococcal fermentation. Recently the production of HA via recombinant systems has received increasing interest due to the avoidance of potential toxins. This work summarizes the research history and current commercial market of HA, and then deeply analyzes the current state of microbial production of HA by Streptococcus zooepide... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5418596 Wed, 16 Nov 2011 05:00:00 +0100 5418596 http://www.medworm.com/index.php?rid=5418597&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F98 Conclusions: To the best of our knowledge, there are no reports on antibiotic production of X. boviebii by medium optimization using RSM. The results strongly support the use of RSM for medium optimization. The optimized medium not only resulted in a 37.8% increase of antibiotic activity, but also reduced the numbers of experiments. The chosen method of medium optimization was efficient, simple and less time consuming. This work will be useful for the development of X. bovienii cultivation process for efficient antibiotic production on a large scale, and for the development of more advanced control strategies on plant diseases. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5418597 Mon, 14 Nov 2011 05:00:00 +0100 5418597 http://www.medworm.com/index.php?rid=5397568&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F96 Conclusions: Here we reported the purification and the biochemical characterization of the novel Shewanella sp. ANA-3 L-arabinose isomerase. Determination of the biochemical properties demonstrated that this enzyme was highly active at low temperatures. The generated T268K mutant displays an increase of the enzyme stability essentially at low pH. These features seem to be very attractive for the bioconversion of D-galactose into D-tagatose at low temperature which is very interesting from industrial point of view. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5397568 Thu, 10 Nov 2011 05:00:00 +0100 5397568 http://www.medworm.com/index.php?rid=5397567&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F97 Conclusions: We have successfully established a new pathway for 1-propanol production by shunting the carbon flux from glycolysis. To our knowledge, it is the first time that this pathway has been utilized to produce 1-propanol in E. coli. The work presented here forms a basis for further improvement in production. We speculate that dragging more carbon flux towards methylglyoxal by manipulating glycolytic pathway and eliminating competing pathways such as lactate generation can further enhance the production of 1-propanol. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5397567 Thu, 10 Nov 2011 05:00:00 +0100 5397567 http://www.medworm.com/index.php?rid=5397569&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F95 Background: Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment samples were collected in the depth (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5397569 Wed, 09 Nov 2011 05:00:00 +0100 5397569 http://www.medworm.com/index.php?rid=5376628&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F94 Conclusion: By taking advantage of its high activity at low temperatures and its acidic pH optimum, this recombinant chitinase will be valuable in various biotechnological applications under low temperature and acidic pH conditions. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376628 Fri, 04 Nov 2011 04:00:00 +0100 5376628 http://www.medworm.com/index.php?rid=5376630&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F92 Many bacterial species contain intracellular nano- and micro-compartments consisting of self-assembling proteins that form protein-only shells. These structures are built up by combinations of a reduced number of repeated elements, from 60 repeated copies of one unique structural element self-assembled in encapsulins of 24 nm to 10,000-20,000 copies of a few protein species assembled in a organelle of around 100-150 nm in cross-section. However, this apparent simplicity does not correspond to the structural and functional sophistication of some of these organelles. They package, by not yet definitely solved mechanisms, one or more enzymes involved in specific metabolic pathways, confining such reactions and sequestering or increasing the inner concentration of unstable, toxics or volatile ... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376630 Thu, 03 Nov 2011 04:00:00 +0100 5376630 http://www.medworm.com/index.php?rid=5376629&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F93 Conclusions: The current study presents a systems biotechnology-based strategy for the engineering of the industrially important yeast P. pastoris combining the use of host specific DNA microarray technologies and physiological studies under well defined environmental conditions. Such studies allowed for the identification of novel targets related with protein trafficking and ergosterol biosynthesis for improved recombinant protein production. Nevertheless, further studies will be required to elucidate the precise mechanisms whereby membrane biogenesis and composition impact on protein secretion in P. pastoris. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376629 Thu, 03 Nov 2011 04:00:00 +0100 5376629 http://www.medworm.com/index.php?rid=5376632&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F90 Conclusion: Y. lipolytica, a generally regarded as safe (GRAS) microorganism is one of the most promising non conventional yeasts for the production of biologically active therapeutic proteins under the control of hydrophobic substrate-inducible promoter. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376632 Wed, 02 Nov 2011 04:00:00 +0100 5376632 http://www.medworm.com/index.php?rid=5376631&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F91 Microalgae have presented themselves as a strong candidate to replace diminishing oil reserves as a source of lipids for biofuels. Here we describe successful modifications of terrestrial plant lipid content which increase productivity overall or shift the balance of lipid production towards lipid varieties more useful for biofuel production. Our discussion ranges from the biosynthetic pathways and rate limiting steps of triacylglyceride formation to enzymes required for the formation of triacylglycerides containing exotic lipids. Secondarily, we discuss techniques for genetic engineering and modification of microalgae which can be combined with insights gained from research in higher plants to aid in the creation of production strains of microalgae. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376631 Wed, 02 Nov 2011 04:00:00 +0100 5376631 http://www.medworm.com/index.php?rid=5376634&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F88 Conclusions: The accessibility of genome information would facilitate research on the genetic engineering of halophilic bacteria including Halomonas sp. TD01. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376634 Tue, 01 Nov 2011 04:00:00 +0100 5376634 http://www.medworm.com/index.php?rid=5376633&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F89 Conclusions: This report represents a significant step towards the goal of cellulosic ethanol production. This engineered yeast consortium displaying a functional mini-cellulosome demonstrated not only the ability to grow on the released sugars from PASC but also a 3-fold higher ethanol production than a similar yeast consortium secreting only the three cellulases. The use of more complex cellulosomal structures may further improve the overall efficiency for ethanol production. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376633 Tue, 01 Nov 2011 04:00:00 +0100 5376633 http://www.medworm.com/index.php?rid=5376635&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F87 Conclusions: Besides the high dissolved CO2 concentration, the excessive addition of MgCO3 was beneficial to promote the succinic acid synthesis. This was the first report investigating the replaceable of gaseous CO2 in the fermentation of succinic acid. The results obtained in this study may be useful for reducing the cost of succinic acid fermentation process. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376635 Mon, 31 Oct 2011 04:00:00 +0100 5376635 http://www.medworm.com/index.php?rid=5376636&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F86 Conclusion: The LcsB region can be used as a functional scaffold to target the heterologous proteins to the cell surfaces of lactic acid bacteria in vitro and in vivo, and has also the potential for biotechnological application. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5376636 Fri, 28 Oct 2011 04:00:00 +0100 5376636 http://www.medworm.com/index.php?rid=5356055&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F85 Conclusion: In this study, we show that a fast approach to determine a minimal set of strain characteristic parameters based on easy-to-do batch cultivations with methanol pulses is generally applicable for different P. pastoris strains and that dynamic fed batch strategies can be designed on the specific substrate uptake rate without running the risk of methanol accumulation. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5356055 Thu, 27 Oct 2011 04:00:00 +0100 5356055 http://www.medworm.com/index.php?rid=5344048&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F84 Conclusions: Our characterization of pol beta from a model fish organism contributes to the study of the function and evolution of DNA polymerases, which are emerging as important cellular targets for chemical intervention in the development of anticancer agents. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5344048 Fri, 21 Oct 2011 04:00:00 +0100 5344048 http://www.medworm.com/index.php?rid=5344049&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F83 Conclusions: The mtl operon promoter (PmtlA) is a strong promoter that reached a maximum of 13,000 Miller units with lacZ as a reporter on low copy plasmids. It is tightly regulated by just one copy of the mtlR gene on the chromosome and subject to CCR. CCR can be switched off by mutations in MtlR and the glucose transporter. These properties and the low costs of the inducers, i.e. mannitol and glucitol, make the promoter ideal for designing regulated expression systems. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5344049 Thu, 20 Oct 2011 04:00:00 +0100 5344049 http://www.medworm.com/index.php?rid=5331400&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F82 Conclusions: Since the cost and the quality of teicoplanin production depend mainly on the fermentation process, we developed a robust and scalable fermentation process by using an industrial medium in which a complex composition can be modulated by the combined addition of suitable precursors. This work was performed in the wild-type strain ATCC 31121, which has a clear genetic background. This is important for starting a rational improvement program and also helps to better control teicoplanin production during process and strain development. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5331400 Tue, 18 Oct 2011 04:00:00 +0100 5331400 http://www.medworm.com/index.php?rid=5331402&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F80 Conclusions: A functional alternative to the pathogenic rhamnolipid producer P. aeruginosa was constructed and characterized. P. putida KT24C1 pVLT31_rhlAB featured the highest yield and titer reported from heterologous rhamnolipid producers with glucose as carbon source. Notably, rhamnolipid production was uncoupled from biomass formation, which allows optimal distribution of resources towards rhamnolipid synthesis. The results are discussed in the context of rational strain engineering by using the concepts of synthetic biology like chassis cells and orthogonality, thereby avoiding the complex regulatory programs of rhamnolipid production existing in the natural producer P. aeruginosa. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5331402 Mon, 17 Oct 2011 04:00:00 +0100 5331402 http://www.medworm.com/index.php?rid=5331401&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F81 Solar-powered polyester production is proved feasible by causing microalgae to express enzymes borrowed from specialized bacteria, to result in biosynthesis of poly-3-hydroxybutyrate. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5331401 Mon, 17 Oct 2011 04:00:00 +0100 5331401 http://www.medworm.com/index.php?rid=5311619&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F79 Conclusions: From the analysis of protein yield, solubility and biological activity of the four model proteins produced alone or along the chaperones, we conclude that GroELS impacts on yield and quality of aggregation-prone proteins with intrinsic determinants but not on thermally induced protein aggregation. No effective modifications of protein solubility have been observed, but significant stabilization of small (encapsulable) substrates and moderate chaperone-induced degradation of larger (excluded) polypeptides. These findings indicate that the activities of this chaperone pair in the context of actively producing recombinant bacteria discriminate between intrinsic and thermally-induced protein aggregation, and that the side effects of GroELS overproduction might be determined by sub... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5311619 Wed, 12 Oct 2011 04:00:00 +0100 5311619 http://www.medworm.com/index.php?rid=5294081&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F77 Conclusions: This is the first report concerning the heterologous expression of alkaline alpha-amylase in B. subtilis, and the obtained results make it feasible to achieve the industrial production of alkaline alpha-amylase with the recombinant B. subtilis. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5294081 Fri, 07 Oct 2011 04:00:00 +0100 5294081 http://www.medworm.com/index.php?rid=5294082&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F76 Conclusions: The results in this paper provide a systematic understanding of the benefits and limitations of bioelectrochemical techniques for biochemical production and highlight how electrical enhancement can impact cellular metabolism and biochemical production. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5294082 Mon, 03 Oct 2011 04:00:00 +0100 5294082 http://www.medworm.com/index.php?rid=5269812&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F75 Conclusions: The monitoring system showed correlation between wax ester synthesis pattern and luminescent signal. The system shows potential for real-time screening purposes and studies on bacterial wax esters, revealing new aspects to dynamics and role of wax ester metabolism in bacteria. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5269812 Fri, 30 Sep 2011 04:00:00 +0100 5269812 http://www.medworm.com/index.php?rid=5269813&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F74 Conclusion: The polysaccharide isolated from sponge-associated B. licheniformis has several features that provide a tool for better exploration of novel anti-biofilm compounds. Inhibiting biofilm formation of a wide range of bacteria without affecting their growth appears to represent a special feature of the polysaccharide described in this report. Further research on such surface-active compounds might help developing new classes of anti-biofilm molecules with broad spectrum activity and more in general will allow exploring of new functions for bacterial polysaccharides in the environment. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5269813 Tue, 27 Sep 2011 04:00:00 +0100 5269813 http://www.medworm.com/index.php?rid=5257740&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F73 Processing bodies (PBs) and stress granules (SGs) are two highly conserved cytoplasmic ribonucleoprotein foci that contain translationally repressed mRNAs together with proteins from the mRNA metabolism. Interestingly, they also share some common features with other granules, including the prokaryotic inclusion bodies. Although the function of PBs and SGs remains elusive, major advances have been done in unraveling their composition and assembly by using the yeast Saccharomyces cerevisae. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5257740 Sat, 24 Sep 2011 04:00:00 +0100 5257740 http://www.medworm.com/index.php?rid=5218268&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F72 Conclusions: A number of parameters in recombinant protein production were seen to influence the surface expression of the model protein with respect both to the productivity and to the display on the individual cell. The choice of medium and the cell design to remove proteolytic cleavage were however the most important. Both fed-batch and batch processing can be successfully used, but prolonged batch processing is probably only possible if the chosen strain has a low acetic acid production. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5218268 Wed, 14 Sep 2011 04:00:00 +0100 5218268 http://www.medworm.com/index.php?rid=5204913&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F70 Conclusions: The metabolic simulation demonstrated that the poor productivity of S. cerevisiae was improved by the introduction of E. coli genes to compensate the structural difference. This suggested that gene supplementation is a promising strategy for the metabolic engineering of S. cerevisiae to produce higher alcohols which should be the next challenge for the synthetic bioengineering of S. cerevisiae for the efficient production of higher alcohols. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5204913 Thu, 08 Sep 2011 04:00:00 +0100 5204913 http://www.medworm.com/index.php?rid=5156669&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F68 Conclusions: : This study not only provides a catalogue of the prevalent proteins secreted by T. reesei in the two media, but the results also suggest that production of hydrolytic enzymes using unconventional carbon sources, such as components in spent hydrolysates, deserves further attention in the future. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5156669 Mon, 22 Aug 2011 23:00:00 +0100 5156669 http://www.medworm.com/index.php?rid=5117886&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F67 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5117886 Wed, 10 Aug 2011 23:00:00 +0100 5117886 http://www.medworm.com/index.php?rid=5117887&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F66 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5117887 Mon, 08 Aug 2011 23:00:00 +0100 5117887 http://www.medworm.com/index.php?rid=5106254&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F65 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5106254 Sun, 07 Aug 2011 23:00:00 +0100 5106254 http://www.medworm.com/index.php?rid=5103045&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F64 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5103045 Thu, 04 Aug 2011 23:00:00 +0100 5103045 http://www.medworm.com/index.php?rid=5094740&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F62 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5094740 Wed, 03 Aug 2011 23:00:00 +0100 5094740 http://www.medworm.com/index.php?rid=5094739&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F63 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5094739 Wed, 03 Aug 2011 23:00:00 +0100 5094739 http://www.medworm.com/index.php?rid=5094741&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F61 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5094741 Tue, 02 Aug 2011 23:00:00 +0100 5094741 http://www.medworm.com/index.php?rid=5085473&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F60 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5085473 Sun, 31 Jul 2011 23:00:00 +0100 5085473 http://www.medworm.com/index.php?rid=5076874&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F58 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5076874 Thu, 28 Jul 2011 23:00:00 +0100 5076874 http://www.medworm.com/index.php?rid=5076873&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F59 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5076873 Thu, 28 Jul 2011 23:00:00 +0100 5076873 http://www.medworm.com/index.php?rid=5068874&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F57 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5068874 Mon, 25 Jul 2011 23:00:00 +0100 5068874 http://www.medworm.com/index.php?rid=5048889&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F55 ${item.shortDescription} (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5048889 Wed, 20 Jul 2011 23:00:00 +0100 5048889 http://www.medworm.com/index.php?rid=5035708&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F54 Conclusions: The combination of the use of a fat-contaminated soil, enrichment of prokaryotic DNA and a three-step screening strategy led to a high number of lipase-producing clones in the metagenomic library. The most notable properties of the new lipase that was isolated and characterized were a high specific activity against long chain triacylglycerols, activity and stability over a wide range of pH values, good thermal stability and stability in water-miscible organic solvents and at high salt concentrations. These characteristics suggest that this lipase has potential to perform well in biocatalytic processes, such as for hydrolysis and synthesis reactions involving long-chain triacylglycerols and fatty acid esters. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=5035708 Thu, 14 Jul 2011 23:00:00 +0100 5035708 http://www.medworm.com/index.php?rid=4992076&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F52 Conclusions: The transcriptional changes caused by the deletion of cra gene did not affect the activity of the central carbon metabolism, suggesting that Cra does not act alone; rather it interacts with other pleiotropic regulators to create a network of metabolic effects. An unexpected outcome of this work is the finding that cra deletion caused transcription inhibition of the bet operon in E. coli K-12 (JM109) but did not affect this operon transcription in E. coli B (BL21). This property, together with the insensitivity to high glucose concentrations, makes this the E. coli B (BL21) strain more resistant to environmental changes. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4992076 Wed, 29 Jun 2011 23:00:00 +0100 4992076 http://www.medworm.com/index.php?rid=4992075&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F53 Conclusions: By using this optimized expression and purification procedure fairly large amounts of good-quality HIV-1Pr recombinant enzyme can be produced at the lab-scale and thus used for further biochemical studies. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4992075 Wed, 29 Jun 2011 23:00:00 +0100 4992075 http://www.medworm.com/index.php?rid=4975036&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F49 Conclusion: The attributes of enhanced biosurfactant and esterase production by hyper-producing recombinant strains have many utilities from industrial viewpoint. This study for the first time has shown a possible association between biosurfactant production and esterase activity in any Bacillus species. Biosurfactant-esterase complex has been found to have powerful emulsification properties, which shows promising bioremediation, hydrocarbon biodegradation and pharmaceutical applications. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4975036 Sun, 26 Jun 2011 23:00:00 +0100 4975036 http://www.medworm.com/index.php?rid=4967955&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F47 Conclusions: A novel 34S labeling procedure that enables in vivo quantification of intracellular fluxes of recombinant protein under "production like" conditions is described. Subsequent sensitivity analysis of the fluxes by using MATLAB, indicate the most promising approaches for strain improvement towards increased secretion. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4967955 Sat, 25 Jun 2011 23:00:00 +0100 4967955 http://www.medworm.com/index.php?rid=4967954&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F48 Conclusions: It is concluded that high level production and intrinsic slow HBsAg VLP assembly kinetics are leading to retention and accumulation of the antigen in the endoplasmic reticulum where it assembles at least partly into defined lamellar structures. Further transport of HBsAg to the Golgi apparatus is impaired thus leading to secretory pathway disfunction and the formation of an extended endoplasmic reticulum which bulges into irregular cloud-shaped formations. As VLPs were not found within the cells it is concluded that the VLP assembly process must take place during down-stream processing after detergent-mediated disassembly of HBsAg lamellas and subsequent reassembly of HBsAg into spherical VLPs. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4967954 Sat, 25 Jun 2011 23:00:00 +0100 4967954 http://www.medworm.com/index.php?rid=4959529&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F46 Conclusion: The use of identical expression strategies for highly similar genes led to very different mRNA levels. The data indicate that this difference is primarily caused by translational effects that are likely to affect both mRNA synthesis rates and mRNA stability. These translational effects thus seem to be a dominant determinant for the success of gene expression efforts in lactobacilli. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4959529 Tue, 21 Jun 2011 23:00:00 +0100 4959529 http://www.medworm.com/index.php?rid=4951733&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F45 Background: The robustness of Saccharomyces cerevisiae in facilitating industrial-scale production of ethanol extends its utilization as a platform to synthesize other metabolites, both native and of heterologous origins. Metabolic engineering strategies, typically via pathway overexpression and deletion, continue to play a key role for optimizing the conversion efficiency of substrates into the desired products. However, chemical production titer or yield remains difficult to predict based on reaction stoichiometry and mass balance. We sampled a large space of data of chemical production from S. cerevisiae, and developed a statistics-based model to calculate production yield using input variables that represent the number of enzymatic steps in the key biosynthetic pathway of interest, met... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4951733 Mon, 20 Jun 2011 23:00:00 +0100 4951733 http://www.medworm.com/index.php?rid=4920497&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F44 Conclusions: Beside the necessary task of updating, this work analyses in detail some applicative aspects of the single-domain antibodies that have been overseen in the past, such as their efficacy as co-crystallization chaperones, protein aggregation controllers, enzyme activity tuners, and the specificities of the unconventional single-domain fragments. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4920497 Wed, 08 Jun 2011 23:00:00 +0100 4920497 http://www.medworm.com/index.php?rid=4910791&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F43 Conclusion: Yeast-mediated biopurification provides a dynamic method to prepare high purity of L-arabinose from the feedstock solution xylose mother liqour, with cost-effective and high-performance properties. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4910791 Mon, 06 Jun 2011 23:00:00 +0100 4910791 http://www.medworm.com/index.php?rid=4891979&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F42 Conclusions: This tested single step purification is capable of producing proteins with high quantity and purity. It can greatly reduce the cost and time, and thus provides application potentials for both industrial scale up and laboratorial usage. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4891979 Wed, 01 Jun 2011 23:00:00 +0100 4891979 http://www.medworm.com/index.php?rid=4882892&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F41 Conclusions: The high stability of EstCS2 in organic solvents and its activity towards esters of ketoprofen and tertiary alcohols, and in polyurethane suggests that it has potential uses for many applications in biotransformation and bioremediation. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4882892 Sat, 28 May 2011 23:00:00 +0100 4882892 http://www.medworm.com/index.php?rid=4861689&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F40 Conclusions: The ability to grow in media with glucose as the sole carbon source indicated that Trichoderma Deltapgi1 mutants were able to use the pentose phosphate pathway. But, they did not increase the expression of gpdh. Morphological characteristics were the result of the pgi1 deletion. Deletion of pgi1 in Rut-C30 increased cellulase production, but only under repressing conditions. This increase resulted partly from the deletion itself and partly from a genetic interaction with the cre1-1 mutation. The lower cellulase activity of these mutants in media with lactose could be attributed to a reduced ability to hydrolyse this sugar but not to an effect on the expression of xyr1. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4861689 Mon, 23 May 2011 23:00:00 +0100 4861689 http://www.medworm.com/index.php?rid=4847514&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F38 Conclusion: We have designed an efficient medium for large scale production of heterologous proteins by Y. lipolytica. The optimized medium GNY is suitable for the production of hIFN alpha2b with the advantage that no complex nitrogen sources with non-defined composition were required. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4847514 Thu, 19 May 2011 23:00:00 +0100 4847514 http://www.medworm.com/index.php?rid=4847513&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F39 Conclusion: The present result shows the complicated nature of the metabolic regulation for the fermentation characteristics upon phosphate limitation, acidic condition, and nitrogen limitation based on the transcript levels of selected genes. The result implies that the regulations under phosphate limitation, acidic condition, and nitrogen limitation, which occur typically at the late growth phase of the batch culture, are interconnected through RpoS and RpoD together with Pho genes. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4847513 Thu, 19 May 2011 23:00:00 +0100 4847513 http://www.medworm.com/index.php?rid=4840182&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F37 Conclusions: Inefficient translocation of MuHN and MeH precursors through ER membrane is a bottleneck for high-level expression in yeast. Overexpression of these recombinant proteins induces the UPR's cytosolic counterpart, the UPR-Cyto, which represent a subset of proteins involved in the heat-shock response. The involvement of eEF1A may explain the mechanism by which only large chaperones, but not small Hsps are upregulated during this stress response. Our study highlights important differences between viral surface protein expression in yeast and mammalian cells at the first stage of secretory pathway. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4840182 Wed, 18 May 2011 23:00:00 +0100 4840182 http://www.medworm.com/index.php?rid=4840183&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F36 Conclusions: In silico predictions of beneficial gene deletions were verified experimentally. The chosen single and multiple gene deletions affected beneficially the natural triacylglycerol metabolism of A. baylyi ADP1. This study demonstrates the importance of single gene deletions in triacylglycerol metabolism, and proposes Acinetobacter sp. ADP1 as a model system for bioenergetic studies regarding metabolic engineering. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4840183 Tue, 17 May 2011 23:00:00 +0100 4840183 http://www.medworm.com/index.php?rid=4840184&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F35 Conclusions: The use of mutants that reduce the specific substrate uptake rate seems to be a versatile tool for overcoming some of the difficulties in the production of integral membrane spanning proteins. A set of strains with mutations in the glucose uptake system and with a lower acetic acid formation were able to produce three out of five membrane proteins that it was not possible to produce with the corresponding wild type. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4840184 Mon, 16 May 2011 23:00:00 +0100 4840184 http://www.medworm.com/index.php?rid=4828338&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F34 Conclusion: The results demonstrated that design of experiments can be utilized for a rapid optimization of initial culture conditions and that P. pastoris is highly capable of producing and secreting functional single-chain antibody fragments at temperatures as low as 11 degrees C. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4828338 Sun, 15 May 2011 23:00:00 +0100 4828338 http://www.medworm.com/index.php?rid=4828339&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F32 Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying parameters, such as expression vector, media composition, growth temperature and chaperone co-expression. Recently several new approaches for the genome-scale engineering of E. coli to enhance recombinant protein expression have been developed. These methodologies now enable the generation of optimized E. coli expression strains in a manner analogous to metabolic engineering for the synthesis of low-molecular-weight compounds. In this short review, we provide an overview of strain engineering approache... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4828339 Fri, 13 May 2011 23:00:00 +0100 4828339 http://www.medworm.com/index.php?rid=4812423&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F31 Conclusions: DTT highly improved both, periplasmic and cytoplasmic accumulation and activity of RI at low synthesis rate, i.e. in constructs harbouring weak recombinant synthesis rate stipulating genetic elements together with cultivation at low temperature. In a stirred bioreactor environment RI folding was strongly improved by repeated pulse addition of DTT at low aeration conditions. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4812423 Mon, 09 May 2011 23:00:00 +0100 4812423 http://www.medworm.com/index.php?rid=4788494&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F30 Conclusions: Plasmid pSM429 was isolated and characterized, and the regions essential for plasmid replication and stability were determined, helping the development of pSM429-based shuttle vectors. The shuttle vectors pWD and its derivatives could be used as cloning vectors for Pseudoalteromonas, offering new perspectives in the genetic manipulation of Pseudoalteromonas strains. With the aid of pWD-derived vector and its host, the erythromycin resistance gene and the cd gene of a cold-adapted protein were successfully expressed, indicating that the potential use of this system for recombinant protein production, especially for cold-adapted proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4788494 Wed, 04 May 2011 23:00:00 +0100 4788494 http://www.medworm.com/index.php?rid=4753240&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F28 Conclusions: Based on the 16S rRNA gene molecular analysis, eight Gram-positive cocci milk isolates were identified as L. lactis subsp. lactis. One of the strains, L. lactis M4 was able to maintain transformed low molecular weight plasmid vectors and expressed the GFP gene. This strain has the potential to be developed into a new lactococcal host for the expression of heterologous proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4753240 Mon, 25 Apr 2011 23:00:00 +0100 4753240 http://www.medworm.com/index.php?rid=4742092&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F24 Conclusion: Compared to insect cells, P. pastoris is easier to handle, can be grown at lower cost, and can be expressed quicker at a large scale. Yeast, P. pastoris, and insect cells are all effective expression systems for GPCRs. The results of the present study strongly suggested that protein expression in P. pastoris can be applied to the structural and biochemical studies of GPCRs. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4742092 Thu, 21 Apr 2011 23:00:00 +0100 4742092 http://www.medworm.com/index.php?rid=4742091&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F25 Conclusions: The study illustrates that the recruitment of a pleiotropic mutation, whose effects might reach deep into physiological regulation, effectively makes P. putida KT2440 and KT2442 two different strains in terms of mcl-PHA production. The differences include the onset of mcl-PHA production (nitrogen limitation) and the resulting strain performance (growth rate). It remains difficult to predict a priori where such major changes might occur, as illustrated by the comparable behavior on octanoate. Consequently, experimental data on mcl-PHA production acquired for P. putida KT2442 cannot always be extrapolated to KT2440 and vice versa, which potentially reduces the body of available knowledge for each of these two model strains for mcl-PHA production substantially. (Source: Microbial... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4742091 Thu, 21 Apr 2011 23:00:00 +0100 4742091 http://www.medworm.com/index.php?rid=4742090&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F26 Conclusions: The present data demonstrate that Nalpha-acetylated Talpha1 can be efficiently produced in recombinant E. coli. This bioprocess could be used as an alternative to chemosynthesis for the production of Talpha1. The described methodologies may also be helpful for the biosynthesis of similar peptides. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4742090 Thu, 21 Apr 2011 23:00:00 +0100 4742090 http://www.medworm.com/index.php?rid=4719476&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F23 Conclusions: It was possible to distinguish between live, metabolic active, depolarized, dormant, and dead cells and to discriminate between high and low productive cells. The methods were shown suitable tools for process monitoring at single cell level allowing a better process understanding, increase robustness and form a firm basis for physiology-based analysis and optimization with general application for bioprocess development. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4719476 Thu, 14 Apr 2011 23:00:00 +0100 4719476 http://www.medworm.com/index.php?rid=4704082&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F22 Conclusion: Apart from the accumulated knowledge and the existence of a wealth of equipment and techniques, the results indicate the selection of E. coli for further studies for surface expression of salmonella antigens. Surface expression of the full length protein facing the cell environment was positively proven by standard analysis, and the FACS signal comparison to expression in Staphylococcus carnosus shows that the distribution of the surface protein on each cell was comparatively very narrow in E. coli, the E. coli outer membrane molecules can serve as an adjuvant for the surface antigenic proteins and multimeric forms of the SefA protein were detected which would probably be positive for the realisation of a strong antigenic property. The detection of specific and similar proteoly... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4704082 Sun, 10 Apr 2011 23:00:00 +0100 4704082 http://www.medworm.com/index.php?rid=4692817&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F21 Conclusion: beta-Galactosidase activity level precisely correlated with the interaction force of tested protein pairs, and very weak beta-galactosidase expression was detected throughout the control groups, which demonstrated the feasibility of this system for studying protein interactions. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4692817 Fri, 08 Apr 2011 23:00:00 +0100 4692817 http://www.medworm.com/index.php?rid=4683407&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F20 Conclusion: Because of its attractive properties XynD might be considered for biotechnological applications. Moreover, XOS hydrolysis suggested that XynD possess four catalytic subsites with a high energy of interaction with the substrate and a fifth subsite with a small energy of interaction, according to the GH10 xylanase literature data. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4683407 Mon, 04 Apr 2011 23:00:00 +0100 4683407 http://www.medworm.com/index.php?rid=4663488&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F19 Conclusions: The genetic correlation exists between central carbon metabolism and DNA replication in the model Gram-negative bacterium, E. coli. This link exists at the steps of initiation and elongation of DNA replication, indicating the important global correlation between metabolic status of the cell and the events leading to cell reproduction. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4663488 Wed, 30 Mar 2011 23:00:00 +0100 4663488 http://www.medworm.com/index.php?rid=4631143&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F18 Conclusions: We have discovered a set of genotypic adaptations that confer increased tolerance to exogenous isobutanol stress. Our results are immediately useful to further efforts to engineer more isobutanol tolerant host strains of E. coli for isobutanol production. We suggest that rpoS and post-transcriptional regulators, such as hfq, RNA helicases, and sRNAs may be interesting mutagenesis targets for future global phenotype engineering. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4631143 Fri, 25 Mar 2011 00:00:00 +0100 4631143 http://www.medworm.com/index.php?rid=4621752&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F17 Conclusions: We show that addition of a range of antifoaming agents to shake flask cultures of P. pastoris increases the total yield of the recombinant protein being produced. This is not only a simple method to increase the amount of protein in the culture, but our study also provides insight into how antifoams interact with microbial cell factories. Two mechanisms are apparent: one group of antifoams (Antifoam A, Antifoam C and J673A) increases the specific yield of GFP by increasing the total amount of protein produced and secreted per cell, whilst the second (P2000 or SB2121) increases the total yield by increasing the density of the culture. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4621752 Tue, 22 Mar 2011 00:00:00 +0100 4621752 http://www.medworm.com/index.php?rid=4544562&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F14 Conclusions: Our strategy describes a novel and fast approach to determine strain specific parameters of a recombinant Pichia pastoris strain to set up feeding profiles solely based on the specific substrate uptake rate. This approach is generic and will allow application to other products and other hosts. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4544562 Thu, 03 Mar 2011 00:00:00 +0100 4544562 http://www.medworm.com/index.php?rid=4527459&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F13 Conclusions: Overall, our results showed that under oxygen-limited and non oxygen-limited conditions, alginate production and its molecular mass are linked to the OTRmax, independently of the DOT of the culture. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4527459 Sun, 27 Feb 2011 00:00:00 +0100 4527459 http://www.medworm.com/index.php?rid=4516832&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F12 Conclusions: The current study demonstrates advantages of the usage of strictly controlled continuous cultivation methods combined with multi-omics approach for quantitative understanding of amino acid and energy metabolism of Lactococcus lactis which is a valuable new knowledge for development of balanced growth media, gene manipulations for desired product formation etc. Moreover, collected dataset is an excellent input for developing metabolic models. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4516832 Thu, 24 Feb 2011 00:00:00 +0100 4516832 http://www.medworm.com/index.php?rid=4501242&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F11 Conclusions: Pseudomonas putida KT2440 is a good cell factory for the production of scFvs, and the broad host range constructs we have produced allow yield assessment in a number of different expression hosts when yields in one initially selected are insufficient. High cell density cultivation and further optimization and refinement of the KT2440 cell factory will achieve additional increases in the yields of scFvs. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4501242 Mon, 21 Feb 2011 00:00:00 +0100 4501242 http://www.medworm.com/index.php?rid=4489094&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F10 Conclusion: The increase of capsular polysaccharide titre confirmed the validity of the proposed fermentation strategy and opened the way to the use of the microfiltration bioreactor for the biotechnological production of chondroitin. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4489094 Wed, 16 Feb 2011 00:00:00 +0100 4489094 http://www.medworm.com/index.php?rid=4477165&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F9 Conclusions: This has been the first report where a terminally attached self-assembling beta peptide ELK16 can promote the formation of active inclusion bodies or active protein aggregates in E. coli. It has the potential to render E. coli and other recombinant hosts more efficient as microbial cell factories for protein production. Our observation might also provide hints for protein aggregation-related diseases. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4477165 Tue, 15 Feb 2011 00:00:00 +0100 4477165 http://www.medworm.com/index.php?rid=4464205&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F8 Conclusions: LOOS1 is a new type of protein with disrupting activity on cellulose. LOOS1 binds polysaccharides, and given its enhancing properties on the action of hydrolytic enzymes, LOOS1 represents a potential additive in the production of fermentable sugars from lignocellulose. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4464205 Fri, 11 Feb 2011 00:00:00 +0100 4464205 http://www.medworm.com/index.php?rid=4459486&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F6 Conclusions: The impact of this study can be seen in the usage of a gram-positive organism for the production of pyrogen-free self-assembling recombinant S-layer/allergen fusion protein with great relevance for the development of vaccines for immunotherapy of atopic allergy. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4459486 Thu, 10 Feb 2011 00:00:00 +0100 4459486 http://www.medworm.com/index.php?rid=4459485&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F7 Conclusions: This new chitosanase production system is of interest for biotechnology as only common media components are used and enzyme of high degree of purity is obtained directly in the culture supernatant. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4459485 Thu, 10 Feb 2011 00:00:00 +0100 4459485 http://www.medworm.com/index.php?rid=4445452&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F5 Conclusion: In this study, we developed a biological method for the purification of xylose from xylose mother liquor and subsequent preparation of xylitol by C. maltosa-mediated biohydrogenation of xylose. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4445452 Mon, 07 Feb 2011 00:00:00 +0100 4445452 http://www.medworm.com/index.php?rid=4411266&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F4 Conclusions: Using the developed molecular tools, new yeast strains were generated with increased capacity to produce plant terpenoids. The approach taken and the durability of the strains allow successive rounds of improvement to maximize yields. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4411266 Fri, 28 Jan 2011 00:00:00 +0100 4411266 http://www.medworm.com/index.php?rid=4405230&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F3 Conclusion: The present result demonstrated how the fermentation characteristics could be explained by the transcript levels of metabolic pathway genes as well as global regulators in relation to the knockout of such single genes as cyoA, cydB, fnr, and fur, and clarified the complex gene network regulation in relation to glycolysis, TCA cycle, respiration, and N-regulated pathways. The present result is quite important in understanding the metabolic regulation for metabolic engineering. Moreover, the present result may be useful in improving the specific glucose consumption rate and activation of the TCA cycle by modulating the respiratory chain genes and the related global regulators. The result obtained under N-limited condition may be useful for the heterologous protein production unde... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4405230 Thu, 27 Jan 2011 00:00:00 +0100 4405230 http://www.medworm.com/index.php?rid=4330522&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F2 Conclusions: Our metabolomic approach revealed one of the molecular events underlying the response to acetic acid and focuses attention on the non-oxidative PPP as a target for metabolic engineering. An important challenge for metabolic engineering is identification of gene targets that have material importance. This study has demonstrated that metabolomics is a powerful tool to develop rational strategies to confer tolerance to stress through genetic engineering. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4330522 Mon, 10 Jan 2011 00:00:00 +0100 4330522 http://www.medworm.com/index.php?rid=4322265&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F10%2F1%2F1 Conclusions: Our results show that the production of eukaryotic proteins with multiple disulfide bonds in the cytoplasm of E.coli is possible. The required exogenous components can be put onto a single plasmid vector allowing facile transfer between different prokaryotic strains. These results open up new avenues for the use of E.coli as a microbial cell factory. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4322265 Fri, 07 Jan 2011 00:00:00 +0100 4322265 http://www.medworm.com/index.php?rid=4300399&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F101 Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire specific traits of interest in biomedicine, for instance through the addition of cell targeting agents for desired biodistribution and specific delivery of associated drugs. However, fully artificial constructs would be highly desirable regarding finest tuning and adaptation to precise therapeutic purposes. Although engineering of protein assembling is still in its infancy, arising principles and promising strategies of protein manipulation point out the rational construction of n... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4300399 Thu, 30 Dec 2010 00:00:00 +0100 4300399 http://www.medworm.com/index.php?rid=4264855&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F100 Conclusion: Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that Lactobacillus plantarum WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4264855 Fri, 17 Dec 2010 00:00:00 +0100 4264855 http://www.medworm.com/index.php?rid=4252684&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F99 Conclusions: Compared to previously reported methods, our culture technique with the MSR-1 strain significantly increased cell density, cell yield, and magnetosome yield in a shorter time window and thus reduced the cost of production. The cell density and magnetosome yield reported here are the highest so far achieved with a magnetotactic bacteria. Refinement of this technique will enable further increase of cell density and magnetosome yield. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4252684 Sun, 12 Dec 2010 00:00:00 +0100 4252684 http://www.medworm.com/index.php?rid=4232828&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F98 Conclusions: The expression of LigANI and LipL32 in P. pastoris resulted in a significant increase in yield compared to expression in E. coli. In addition, the proteins were secreted, allowing for easy purification, and retained the antigenic characteristics of the native proteins, demonstrating their potential application as subunit vaccine candidates. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4232828 Mon, 06 Dec 2010 00:00:00 +0100 4232828 http://www.medworm.com/index.php?rid=4224702&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F97 Conclusions: We propose that the localization of the model protein Peb1 in the growth medium of E. coli is due to active secretion by a still unknown pathway of E. coli. The secretion apparently is a two-step process involving a periplasmic step and the TAR regions. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4224702 Thu, 02 Dec 2010 00:00:00 +0100 4224702 http://www.medworm.com/index.php?rid=4205670&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F96 Conclusions: Our work represents the first report of direct microbial production of enantiomerically pure 3HV from a single carbon source. Continued engineering of host strains and pathway enzymes will ultimately lead to more economical production of chiral 3HV. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4205670 Sat, 27 Nov 2010 00:00:00 +0100 4205670 http://www.medworm.com/index.php?rid=4202905&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F95 Conclusion: In the two chemostat conditions, used to generate biomass for proteomic analysis, mycelia grew with the same rate and with similar glucose-biomass conversion efficiencies. Global gene expression analysis revealed a differential metabolic adaptation, highlighting strategies for energetic supply and biosynthesis of metabolic intermediates required for biomass production and, in LP, for balhimycin biosynthesis. These data, confirming a relationship between primary metabolism and antibiotic production, could be used to increase antibiotic yield both by rational genetic engineering and fermentation processes improvement. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4202905 Fri, 26 Nov 2010 00:00:00 +0100 4202905 http://www.medworm.com/index.php?rid=4196490&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F92 Conclusions: In this work we present a new secretion tag that combines several advantages for the production of recombinant proteins in E. coli. Domain D of S. aureus protein A protects the protein of interest against N-terminal degradation, increases target protein solubility and enables a straight-forward purification of the recombinant protein using of IgG columns. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4196490 Tue, 23 Nov 2010 00:00:00 +0100 4196490 http://www.medworm.com/index.php?rid=4196489&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F93 Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4196489 Tue, 23 Nov 2010 00:00:00 +0100 4196489 http://www.medworm.com/index.php?rid=4189682&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F91 Conclusions: We isolated a novel fumarase gene, fumF, from a sequence-based screen of a plasmid metagenomic library from uncultivated marine microorganisms. The properties of FumF protein may be ideal for the industrial production of L-malate under higher temperature conditions. The identification of FumF underscores the potential of marine metagenome screening for novel biomolecules. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4189682 Tue, 23 Nov 2010 00:00:00 +0100 4189682 http://www.medworm.com/index.php?rid=4189684&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F89 Global concerns about climate changes and their association with the use of fossil fuels have accelerated research on biological fuel production. Biological hydrogen production from hemicellulose-containing waste is considered one of the promising avenues. A major economical issue for such a process, however, is the low substrate conversion efficiency. Interestingly, the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus can produce hydrogen from carbohydrate-rich substrates at yields close to the theoretical maximum of the dark fermentation process (i.e., 4 mol H2/mol hexose). The organism is able to ferment an array of mono-, di- and polysaccharides, and is relatively tolerant to high partial hydrogen pressures, making it a promising candidate for exploitation in a biohy... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4189684 Mon, 22 Nov 2010 00:00:00 +0100 4189684 http://www.medworm.com/index.php?rid=4189683&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F90 Conclusions: We gained detailed insights into the metabolism of G. sulfurreducens cells under various electron donor/acceptor conditions using 13C-based metabolic flux analysis. Our results can be used for the development of G. sulfurreducens as a chassis for a variety of applications including bioremediation and renewable biofuel production. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4189683 Mon, 22 Nov 2010 00:00:00 +0100 4189683 http://www.medworm.com/index.php?rid=4182783&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F88 Conclusions: It was shown to be useful to simulate the main metabolism of E.coli for understanding metabolic changes inside the cell in response to specific pathway gene knockouts, considering the whole main metabolic pathways. The comparison of the simulation result with the experimental data indicates that the present model could simulate the effect of the specific gene knockouts to the changes in the metabolisms to some extent. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4182783 Fri, 19 Nov 2010 00:00:00 +0100 4182783 http://www.medworm.com/index.php?rid=4175302&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F87 Conclusions: A Saccharomyces cerevisiae expression system has enabled the cGMP manufacture of an animal-free rTf for industrial cell culture application without the risk of prion and viral contamination, and provides a high-quality platform for the development of transferrin-based therapeutics. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4175302 Wed, 17 Nov 2010 00:00:00 +0100 4175302 http://www.medworm.com/index.php?rid=4162677&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F86 Conclusion: In microfluidic MTPs, pH-controlled batch as well as fed-batch fermentations were successfully performed. The liquid dosing as well as the biomass growth kinetics of the process-controlled fermentations agreed well both in the microscale and laboratory scale. In conclusion, a user-friendly and disposable microfluidic system could be established which allows scaleable, fully controlled and fully monitored fermentations in working volumes below 1 milliliter. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4162677 Sat, 13 Nov 2010 00:00:00 +0100 4162677 http://www.medworm.com/index.php?rid=4142519&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F84 Conclusion: Use of constraints corresponding to different physiological states was found to greatly influence the target predictions given minimization of metabolic adjustment (MOMA) as biological objective function. In vivo verification of the targets, selected based on their predicted metabolic adjustment, successfully led to overproducing strains. Overall, we propose and demonstrate a framework for in silico design and target selection for improving microbial cell factories. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4142519 Mon, 08 Nov 2010 00:00:00 +0100 4142519 http://www.medworm.com/index.php?rid=4124579&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F80 In the last decades, the understanding of inclusion body biology and consequently, of their properties and potential biotechnological applications have dramatically changed. Therefore, the development of new purification protocols aimed to preserve those properties is becoming a pushing demand. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4124579 Mon, 01 Nov 2010 00:00:00 +0100 4124579 http://www.medworm.com/index.php?rid=4124578&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F81 Conclusions: Production of bacteriophage T4 in the semi-continuous, automated SCF/SCI system was efficient and reproducible from cycle to cycle. Synchronization of the host in the first stage prior to infection led to improvements in the specific productivity of phages in the second stage while maintaining the volumetric productivity. These results demonstrate the significant potential of this approach for both upstream and downstream process optimization. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4124578 Mon, 01 Nov 2010 00:00:00 +0100 4124578 http://www.medworm.com/index.php?rid=4105072&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F78 Conclusions: We show that the natural system encoded in SPI-1 only produces high titers of secreted protein for 4-8 hours when the natural psicA promoter is used to drive expression. Secretion efficiency can be high, but declines for charged or large sequences. A quantitative characterization of these constraints will facilitate the effective use and engineering of this system. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4105072 Sun, 24 Oct 2010 23:00:00 +0100 4105072 http://www.medworm.com/index.php?rid=4105071&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F79 Conclusions: Approximately 490 of the 650 determinants of tolerance to acetic acid identified in this work are implicated, for the first time, in tolerance to this weak acid. These are novel candidate genes for genetic engineering to obtain more robust yeast strains against acetic acid toxicity. Among these genes there are number of transcription factors that are documented regulators of a large percentage of the genes found to exert protection against acetic acid thus being considered interesting targets for subsequent genetic engineering. The increase of potassium concentration in the growth medium was found to improve the expression of maximal tolerance to acetic acid, consistent with the idea that the adequate manipulation of nutrient concentration of industrial growth medium can be an... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4105071 Sun, 24 Oct 2010 23:00:00 +0100 4105071 http://www.medworm.com/index.php?rid=4081609&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F76 Conclusions: The BFD-variants with high cofactor binding affinity (wild type, His281Ala, Ser181Thr) obviously extract thiamine from the medium and bind it tightly to the enzyme. This might explain the hampered growth of these clones. In contrast, growth of clones expressing variants with low cofactor binding affinity (Leu476His, Leu476Pro, Leu476Pro-Ser181Thr) is not impaired. Leu476Gln has an intermediate cofactor binding strength, thus, growth of its host strain depends on the specific cultivation conditions. This paper shows that slight differences of the amino acid composition can affect protein expression and cultivation and might require an adaptation of media components. Effects such as the observed are hardly foreseeable and difficult to detect in conventional screening processes. ... Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4081609 Mon, 18 Oct 2010 23:00:00 +0100 4081609 http://www.medworm.com/index.php?rid=4081608&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F77 Conclusions: By using fed batch E. coli fermentation at high cell density, ELP-intein-tagged proteins can be expressed and purified at high yield with low cost. Further, the impact of media components and fermentation design can significantly impact the overall process cost, particularly at large scale. This work thus demonstrates an important advances in the scale up of self-cleaving ELP tag-mediated processes. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4081608 Mon, 18 Oct 2010 23:00:00 +0100 4081608 http://www.medworm.com/index.php?rid=4012158&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F75 Conclusions: The results suggest that E. coli B (BL21) tolerates high glucose concentration not only by its more efficient central carbon metabolism, but also by controlling the glucose transport into the cells regulated by the sRNA SgrS, which may suggest a way to control glucose consumption and increase its efficient utilization. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=4012158 Mon, 27 Sep 2010 23:00:00 +0100 4012158 http://www.medworm.com/index.php?rid=3998327&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F74 Conclusions: All the three major wasp venom allergens were expressed on the yeast surface. A high-level expression, which was observed only for antigen 5, was needed for detection of IgE binding by FACS and for induction of histamine release. The non-modified S. cerevisiae cells did not cause any unspecific reaction in FACS or histamine release assay despite the expression of high-mannose oligosaccharides.In perspective the yeast surface display may be used for allergen discovery from cDNA libraries and possibly for sublingual immunotherapy as the cells can serve as good adjuvant and can be produced in large amounts at a low price. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=3998327 Thu, 23 Sep 2010 23:00:00 +0100 3998327 http://www.medworm.com/index.php?rid=3993035&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F73 Conclusions: This study is the first proposed biotechnological application for Rhodotorula glacialis species, whose oleaginous biomass accumulates high amounts of lipids within a wide range of temperatures through appropriate cultivation C:N ratio. Although R. glacialis DBVPG 4785 is a cold adapted yeast, lipid production occurs over a broad range of temperatures and it can be considered an interesting microorganism for the production of single cell oils. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=3993035 Wed, 22 Sep 2010 23:00:00 +0100 3993035 http://www.medworm.com/index.php?rid=3993036&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F72 Conclusion: The growth of P. haloplanktis to higher cell densities on complex medium is possible. A first fed-batch fermentation strategy could be established which is feasible to be used in lab-scale or for industrial purposes. The substrate concentration of the feeding solution was found to influence the maximal biomass yield considerably. The bottleneck for growing P. haloplanktis to high cell densities still remains the availability of a highly concentrated substrate and the reduction of the substrate complexity. However, our results indicate glutamic acid as a major carbon source, which provides a good basis for further improvement of the fed-batch process. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=3993036 Mon, 20 Sep 2010 23:00:00 +0100 3993036 http://www.medworm.com/index.php?rid=3980359&cid=s_34082_77_f&fid=34082&url=http%3A%2F%2Fwww.microbialcellfactories.com%2Fcontent%2F9%2F1%2F71 Conclusions: The application of the developed protocol allows obtaining bacterial free inclusion bodies suitable for use in mammalian cell cultures and other biological interfaces. (Source: Microbial Cell Factories) Microbial Cell Factories journals http://www.medworm.com/rss/comments.php?id=3980359 Thu, 16 Sep 2010 23:00:00 +0100 3980359