MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Molecular Biology Reports' source. Thu, 09 Feb 2012 16:56:51 +0100 http://www.medworm.com/index.php?rid=5656686&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297689%26dopt%3DAbstract This study suggested that increasing PRLR expression that resulted from the special variant on chicken Z chromosome caused the late-feathering phenotype. PMID: 22297689 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656686 Thu, 02 Feb 2012 05:00:00 +0100 5656686 http://www.medworm.com/index.php?rid=5656685&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297690%26dopt%3DAbstract Authors: Fatehi F, Hosseinzadeh A, Alizadeh H, Brimavandi T, Struik PC Abstract Responses of plants to salinity stress and the development of salt tolerance are extremely complex. Proteomics is a powerful technique to identify proteins associated with a particular environmental or developmental signal. We employed a proteomic approach to further understand the mechanism of plant responses to salinity in a salt-tolerant (Afzal) and a salt-sensitive (Line 527) genotype of barley. At the 4-leaf stage, plants were exposed to 0 (control) or 300 mM NaCl. Salt treatment was maintained for 3 weeks. Total proteins of leaf 4 were extracted and separated by two-dimensional gel electrophoresis. More than 500 protein spots were reproducibly detected. Of these, 44 spots showed significant chan... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656685 Thu, 02 Feb 2012 05:00:00 +0100 5656685 http://www.medworm.com/index.php?rid=5656684&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297691%26dopt%3DAbstract In this study, a meta-analysis was performed to clarify the associations of polymorphisms in CYP1A1 gene with HCC risk. Published literature from PubMed, Embase, CNKI and Wanfang Data were retrieved. Pooled odds ratio (OR) with 95% confidence interval (CI) was calculated using fixed- or random-effects model. Eight studies (1,752 cases and 2,279 controls) for Ile-Val polymorphism and eight studies (933 cases and 1,449 controls) for MspI polymorphism were identified. The results showed that there was no statistically significant association between the Ile-Val polymorphism and HCC risk under all genetic models (co-dominant model: Val/Val vs. Ile/Ile: OR = 1.62, 95% CI 0.96-2.72 and Ile/Val vs. Ile/Ile: OR = 1.15, 95% CI 0.87-1.52; dominant model: OR = 1.25, 95% CI 0.92-1.70; recessive ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656684 Thu, 02 Feb 2012 05:00:00 +0100 5656684 http://www.medworm.com/index.php?rid=5656683&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297692%26dopt%3DAbstract Authors: Wang Q, Chen L, Wang Y, Li W, He L, Jiang H Abstract The ubiquitin/proteasome pathway plays an important role in gametogenesis. Ubiquitin/ribosomal fusion protein is the precursor of mono-ubiquitin. We identified two ubiquitin/ribosomal fusion proteins genes in testis of Eriocheir sinensis. The ubiquitin-ribosomal proteins S27 and L40 are denoted as UBS27 and UBL40, respectively. The full length Es-UBS27 cDNA (565 bp) was cloned using PCR, based upon an initial expressed sequence tag (EST) isolated from a testicular cDNA library and the result of 3' RACE. The sequence contained a 465-nucleotide open reading frame (ORF) that encoded a protein of 154 amino acids. It contained a Ubiquitin (UB) domain and the ribosomal protein S27. The full-length Es-UBL40 cDNA (521 bp) cont... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656683 Thu, 02 Feb 2012 05:00:00 +0100 5656683 http://www.medworm.com/index.php?rid=5656682&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297693%26dopt%3DAbstract Authors: Lu N, Yang Y, Wang Y, Liu Y, Fu G, Chen D, Dai H, Fan X, Hui R, Zheng Y Abstract The polymorphisms of angiotensin-converting enzyme 2 (ACE2) gene have been suggested to be linked to increase risk of essential hypertension in multiple populations. However, the results are still debatable. To assess the association between ACE2 G8970A genetic polymorphism and essential hypertension, we conducted a meta-analysis of case-control studies across different ethnicity. PubMed, Embase, CBM, Wanfang and VIP databases were searched, and a total of 11 separate studies in females and nine separate studies in males met the inclusion criteria. Because ACE2 is on the X chromosome, data for each sex were analyzed separately. The selected studies contained 7,251 (4,472 females/2,779 males) h... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656682 Thu, 02 Feb 2012 05:00:00 +0100 5656682 http://www.medworm.com/index.php?rid=5656681&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297694%26dopt%3DAbstract Authors: Sun Q, Hua J, Wang Q, Xu W, Zhang J, Zhang J, Kang J, Li M Abstract The purpose of this study was to detect the expressions of GRP78 and Bax in human non-small cell lung cancer (NSCLC) tissues, to analyze their correlations with carcinogenesis and the development of NSCLC, and to investigate the relationship of GRP78 expression to metastasis and apoptosis in the NSCLC cell line HCC827. The positive expression rates of GRP78 and Bax in NSCLC lung tissues were 59.7% and 34.7% by RT-PCR, respectively. The mRNA and protein expression levels of GRP78 in NSCLC tissues were significantly higher than that in the relatively normal surrounding lung tissues (p < 0.05); the lesser the degree of tumor differentiation was, the higher the mRNA and protein expression levels of GRP78 ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656681 Thu, 02 Feb 2012 05:00:00 +0100 5656681 http://www.medworm.com/index.php?rid=5656680&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297695%26dopt%3DAbstract In conclusion, this meta-analysis indicates that COMT Val158Met polymorphism may be associated with decreased breast cancer risk in Caucasian population. However, a study with the larger sample size is needed to further evaluated gene-environment interaction on COMT Val158Met polymorphisms and breast cancer risk. PMID: 22297695 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656680 Thu, 02 Feb 2012 05:00:00 +0100 5656680 http://www.medworm.com/index.php?rid=5656691&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22294104%26dopt%3DAbstract Authors: Long SH, Deng X, Wang YF, Li X, Qiao RQ, Qiu CS, Guo Y, Hao DM, Jia WQ, Chen XB Abstract Bast fibre crops are the second most important natural fibre crops following cotton. Of these, flax (Linum ustitatissimum L.) is the most widely planted in the world, with its fibre used for high quality linen textile. A cDNA library of flax bark tissues was constructed with the purpose of identifying genes involved in the Bast fibre development. A total of 2,297 unigene sequences were obtained from 3,200 randomly selected clones of the cDNA library. These sequences were grouped into 155 clusters and 2,142 singletons, which have been submitted to the GenBank databases. By putative functional annotation, 23.3% of these sequences were similar to known proteins in GenBank, 44.0% of these ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656691 Wed, 01 Feb 2012 05:00:00 +0100 5656691 http://www.medworm.com/index.php?rid=5656690&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22294105%26dopt%3DAbstract In this study, we investigated the effects of immature DC (imDC) co-transfected with IL-10 and TGF-beta 1 genes (IL-10-TGF-beta 1-imDC) on inducing immune tolerance. Moreover, we compared the effects of IL-10-TGF-beta 1-imDC with IL-10 transfected imDC (IL-10-imDC) and TGF-beta 1-transfected imDC (TGF-beta 1-imDC), respectively. IL-10-TGF-beta 1-imDC resulted in the down-regulation of MHC class II, CD80 and CD86. IL-10-TGF-beta 1-imDC could induce T cell hyporesponsiveness, and was reluctant to proliferate. IL-10-TGF-beta 1-imDC was more effective than IL-10-imDC and TGF-beta 1-imDC, respectively. In summary, co-expression of IL-10 and TGF-beta 1 affected the immunity of imDCs and enhanced their tolerogenicity. It might be a promising therapy for donor-specific tolerance after organ transp... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656690 Wed, 01 Feb 2012 05:00:00 +0100 5656690 http://www.medworm.com/index.php?rid=5656689&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22294106%26dopt%3DAbstract Authors: Sergentanis TN, Economopoulos KP, Choussein S, Vlahos NF Abstract This meta-analysis aims to examine whether the genotype status of MspI and Ile462Val polymorphisms in Cytochrome-P450 1A1 (CYP1A1) is associated with cervical cancer risk. Eligible case-control studies were identified through search in MEDLINE (end of search: October 2010). Pooled odds ratios (ORs) were appropriately derived from fixed-effects or random effects models. Concerning MspI polymorphism, six studies were eligible (722 cases and 770 controls); four studies were eligible (350 cases and 519 controls) for Ile462Val. MspI polymorphism was associated with elevated cervical cancer risk (for heterozygous TC vs. TT carriers OR = 1.50, 95% CI: 0.93-2.42, random effects; for homozygous CC vs. TT carriers O... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656689 Wed, 01 Feb 2012 05:00:00 +0100 5656689 http://www.medworm.com/index.php?rid=5656688&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22294107%26dopt%3DAbstract In conclusion, our study suggests that the common α1-antichymotrypsin signal peptide -15A/T polymorphism may not be a major risk factor for AD. However, the polymorphism is capable of increasing LOAD risk. PMID: 22294107 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656688 Wed, 01 Feb 2012 05:00:00 +0100 5656688 http://www.medworm.com/index.php?rid=5656687&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22294108%26dopt%3DAbstract In conclusion, we did not found the evidence that the Cys allele at codon 326 of hOGG1 could increase GC risk in our analysis. PMID: 22294108 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656687 Wed, 01 Feb 2012 05:00:00 +0100 5656687 http://www.medworm.com/index.php?rid=5656694&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290286%26dopt%3DAbstract Authors: Chao Z, Wang F, Deng CY, Wei LM, Sun RP, Liu HL, Liu QW, Zheng XL Abstract PCR-RFLP was used to analyze the polymorphisms of MC4R, LEP, H-FABP genes in a swine breed composite (DIV(2)) and 4 swine breeds (Yorkshire, Landrace, Meishan, Bamei). The association study of these polymorphisms with several economic traits was carried out on a DIV(2) population. The results obtained showed that MC4R/TaqI genotype had an effect for average backfat thickness (P < 0.05) and lean meat percentage (P < 0.05). At locus LEP/HinfI animals of AA genotype had lower test daily gain than that of BB (P < 0.01) or AB genotype (P < 0.05). At the H-FABP/HaeIII locus lean meat percentage of the individuals with genotype DD were higher than that with genotype dd (P < 0.05). ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656694 Tue, 31 Jan 2012 05:00:00 +0100 5656694 http://www.medworm.com/index.php?rid=5656693&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290287%26dopt%3DAbstract Authors: Lee YH, Choi SJ, Ji JD, Song GG Abstract The aim of this study was to explore whether vitamin D receptor (VDR) polymorphisms confer susceptibility to psoriasis. Meta-analyses were conducted on the associations between the VDR ApaI, TaqI, BsmI, and FokI polymorphisms and psoriasis. Nine relevant studies on VDR polymorphisms and psoriasis were included in this meta-analysis, which involved 742 psoriasis patients and 715 controls. Meta-analysis indicated an association between the VDR ApaI A allele and psoriasis in Turkish studies (OR = 0.684, 95% CI = 0.475-0.985, p = 0.041). Meta-analysis indicated an association between the BsmI B allele and psoriasis in Asians (OR = 0.636, 95% CI = 0.411-0.984, p = 0.041), and showed a significant association between the FF an... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656693 Tue, 31 Jan 2012 05:00:00 +0100 5656693 http://www.medworm.com/index.php?rid=5656692&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290288%26dopt%3DAbstract In this study, we have reported a full length of small heat shock protein 37 (designated MrHSP37) gene, identified from the transcriptome database of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrHSP37 is 2,425 base pairs in length, and encodes 338 amino acids. MrHSP37 contains a long heat shock protein family profile in the amino acid sequence between 205 and 288. The mRNA expressions of MrHSP37 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction (qRT-PCR). MrHSP37 is highly expressed in hepatopancreas and all the other tissues (walking leg, gills, muscle, stomach, haemocyte, intestine, pleopods, brain and eye stalk) of M. rosenberg... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656692 Tue, 31 Jan 2012 05:00:00 +0100 5656692 http://www.medworm.com/index.php?rid=5656695&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286901%26dopt%3DAbstract The objective of this research was to evaluate a collection of 86 oilseed rape cultivars with 188 simple sequence repeat (SSR) markers to assess the genetic variability, heterotic group identity and relationships within and between the groups identified among the genotypes. A total of 631 alleles at 188 SSR markers were detected including 53 and 84 unique and private alleles respectively, which indicated great richness and uniqueness of genetic variation in these selected cultivars. The mean number of alleles per locus was 3.3 and the average polymorphic information content was 0.35 for all microsatellite loci. Unweighted Pair Group Method with Arithmetic Mean clustering and principal component analysis consistently divided all the cultivars into four distinct groups (I, II, III and IV) wh... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5656695 Sat, 28 Jan 2012 05:00:00 +0100 5656695 http://www.medworm.com/index.php?rid=5619331&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22249446%26dopt%3DAbstract This study demonstrated that combination of cisplatin application with miR-21 downregulation might be a potential strategy for the treatment of human OSCC. PMID: 22249446 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5619331 Sun, 15 Jan 2012 05:00:00 +0100 5619331 http://www.medworm.com/index.php?rid=5619332&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22246941%26dopt%3DAbstract Authors: Cui XW, Xiao W, Ji CB, Tian AY, Zhang J, Zhang SQ Abstract Here we describe the identification of the hedgehog Erinaceus europaeus homologue of a proliferation-inducing ligand (APRIL) of the TNF family (designated heAPRIL). Hedgehog APRIL contains two cysteine residues (Cys(196) and Cys(211)), a furin protease cleavage site and a conserved putative N-glycosylation site (Asn(124)). Real-time quantitative PCR (qPCR) analysis revealed that heAPRIL could be detected in various tissues. MTT assays and flow cytometric analysis revealed that Nus-hesAPRIL and hesAPRIL could promote the survival/proliferation of splenic B cells. Laser scanning confocal microscopy analysis showed GFP-hesAPRIL could successfully bind to the APRIL receptors of lymphocytes. PMID: 22246941 [PubMed -... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5619332 Sat, 14 Jan 2012 05:00:00 +0100 5619332 http://www.medworm.com/index.php?rid=5596567&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22237862%26dopt%3DAbstract In conclusion, DFAT cells is a highly purified stem cell population, and expressed some embryonic stem cell markers like ADSCs, which seems to be a good candidate source of adult stem cells for the future cell replacement therapy. PMID: 22237862 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596567 Thu, 12 Jan 2012 05:00:00 +0100 5596567 http://www.medworm.com/index.php?rid=5596566&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22237863%26dopt%3DAbstract Expression and glucocorticoid-regulation of "Bam", a novel BH3-only transcript in acute lymphoblastic leukemia. Mol Biol Rep. 2012 Jan 12; Authors: Mansha M, Wasim M, Kofler A, Ploner C Abstract Apoptosis is a morphologically defined form of cell death that plays a major role in cell physiology, pathology and cancer therapy. The Bcl-2 family of pro- and anti-apoptotic molecules is a key regulator of this phenomenon, with the sub-family of BH3-only molecules serving as activators and/or facilitators. Apoptosis induced by glucocorticoids (GC) is a central component in the therapy of acute lymphoblastic leukemia (ALL), and defining its molecular basis and that of GC resistance is crucial for therapeutic improvements. We recently identified a novel transcript from the BCL2L11/B... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596566 Thu, 12 Jan 2012 05:00:00 +0100 5596566 http://www.medworm.com/index.php?rid=5596568&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231906%26dopt%3DAbstract Authors: Siegel SR, Mackenzie J, Chaplin G, Jablonski NG, Griffiths L Abstract Multiple sclerosis (MS) is an immune-mediated, demyelinating and neurodegenerative disease of the central nervous system. After traumatic brain injury, it is the leading cause of neurology disability in young adults. Considerable advances have been made in identifying genes involved in MS but the genetic and phenotypic complexity associated with this disease significantly hinders any progress. A novel class of small RNA molecules, microRNAs (miRNAs) has acquired much attention because they regulate the expression of up to 30% of protein-coding genes and may play a pivotal role in the development of many, if not all, complex diseases. Seven published studies investigated miRNAs from peripheral blood monon... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596568 Tue, 10 Jan 2012 05:00:00 +0100 5596568 http://www.medworm.com/index.php?rid=5596571&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228086%26dopt%3DAbstract Authors: Chacon-Cortes D, Haupt LM, Lea RA, Griffiths LR Abstract Genomic DNA obtained from patient whole blood samples is a key element for genomic research. Advantages and disadvantages, in terms of time-efficiency, cost-effectiveness and laboratory requirements, of procedures available to isolate nucleic acids need to be considered before choosing any particular method. These characteristics have not been fully evaluated for some laboratory techniques, such as the salting out method for DNA extraction, which has been excluded from comparison in different studies published to date. We compared three different protocols (a traditional salting out method, a modified salting out method and a commercially available kit method) to determine the most cost-effective and time-efficient m... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596571 Sat, 07 Jan 2012 05:00:00 +0100 5596571 http://www.medworm.com/index.php?rid=5596570&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228087%26dopt%3DAbstract Authors: Chen D, Cheng B, Zhou HY, Li LH Abstract Sevoflurane postconditioning is a potential clinical measure to protect myocardial. This experiment was designed to investigate the efficacy of sevoflurane postconditioning against ischemia-reperfusion injury. A total of 132 Japanese White Rabbits were enrolled into this study. They were underwent 15-, 30-, or 60-min left anterior descending coronary (LAD) artery occlusion, respectively. At the end of LAD artery occlusion, they randomly received a 5-min inhalation of air (control group), 1% sevoflurane (1% sev group), 2% sevoflurane (2% sev group), 4% sevoflurane (4% sev group) or an IV bolus injection of 5 mg/kg of NIM811 [a specific inhibitor of mitochondrial permeability transition pores (mPTP)]. Infarct size was determined afte... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596570 Sat, 07 Jan 2012 05:00:00 +0100 5596570 http://www.medworm.com/index.php?rid=5596569&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22228088%26dopt%3DAbstract Authors: Jiang W, Guo Q, Wu J, Guo B, Wang Y, Zhao S, Lou H, Yu X, Mei X, Wu C, Qiao S, Wu Y Abstract Sodium butyrate (NaBu), a histone deacetylase inhibitor, has been shown to inhibit cell growth, induce cell differentiation and apoptosis in multiple cell lines. In present study, we revealed the dual effects of NaBu in regulating hepatocellular carcinoma (HCC) cells. In two different HCC cell lines, SK-Hep1 and SMMC-7721, low concentrations of NaBu induced a significant increase in cell growth ratio and S-phase cell percentage, accompanied by a reduced p21 Cip1 expression at both mRNA and protein levels, while dissimilarly, high concentrations of NaBu inhibited cell growth and induced G1 arrest through up-regulation of p21 Cip1 and p27 Kip1 protein expression. The reduction of p45... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5596569 Sat, 07 Jan 2012 05:00:00 +0100 5596569 http://www.medworm.com/index.php?rid=5576903&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22223294%26dopt%3DAbstract Authors: Saedi TA, Moeini H, Tan WS, Yusoff K, Daud HM, Chu KB, Tan SG, Bhassu S Abstract White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M. rosenbergii cultured in Malaysia. In the present study, RT-PCR assay was used to detect the causative agents of WTD, M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples. Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from the... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576903 Fri, 06 Jan 2012 05:00:00 +0100 5576903 http://www.medworm.com/index.php?rid=5576902&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22223295%26dopt%3DAbstract Authors: Maharani D, Jung Y, Jung WY, Jo C, Ryoo SH, Lee SH, Yeon SH, Lee JH Abstract Fatty acid composition of meat is becoming more important due to consumer demand for high quality and healthy foods. The present study evaluated the associations of five candidate genes (FABP4, FASN, NR1H3, GH and SCD) with fatty acid composition in Korean cattle (Hanwoo). The g.3691G > A single nucleotide polymorphism (SNP) in the FABP4 gene had significant effects on high myristic acid (C14:0; P < 0.01) and palmitic (C16:0; P < 0.05) in animals having the GG genotype, and high arachidonic acid (C20:4; P < 0.05) in the AA genotype of Hanwoo. The FASN SNP at position g.17924G > A was also significantly associated with myristic acid (P < 0.01). In case of the SCD gene,... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576902 Fri, 06 Jan 2012 05:00:00 +0100 5576902 http://www.medworm.com/index.php?rid=5576907&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22215215%26dopt%3DAbstract Authors: Mao ZG, Zhou J, Wang H, He DS, Xiao WW, Liao GZ, Qiu LB, Zhu YH, Wang HJ Abstract To determine the effect of artesunate (ART) on the rat pituitary adenoma GH3 cell line to evaluate its potential as a novel agent in growth hormone (GH) adenoma and to investigate its underlying mechanisms of action. The MTT assay was used to assess cell proliferation. DAPI staining was used to visualise apoptotic changes in the nucleus. We also analyzed cell apoptosis and cell cycle stage by flow cytometry, semi-quantitative RT-PCR analysis for the expression of GH mRNA and apoptosis-induced factor (AIF) mRNA, analysis of GH protein by western blot, ELISA detection of secreted GH, and the caspase inhibition assay. We found that ART inhibited the proliferation of GH3 cells in a dose- and time... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576907 Thu, 05 Jan 2012 05:00:00 +0100 5576907 http://www.medworm.com/index.php?rid=5576906&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22219085%26dopt%3DAbstract Authors: Johansson M, Duda E, Sremba A, Banks M, Peterson W Abstract The Antarctic krill (Euphausia superba Dana 1852) is widely distributed throughout the Southern Ocean, where it provides a key link between primary producers and upper trophic levels and supports a major commercial fishery. Despite its ecological and commercial importance, genetic population structure of the Antarctic krill remains poorly described. In an attempt to illuminate genetic markers for future population and phylogenetic analysis, five E. superba mitogenomes, from samples collected west of the Antarctic Peninsula, were sequenced using new 454 next-generation sequencing techniques. The sequences, of lengths between 13,310 and 13,326 base pairs, were then analyzed in the context of two previously-published... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576906 Thu, 05 Jan 2012 05:00:00 +0100 5576906 http://www.medworm.com/index.php?rid=5576905&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22219086%26dopt%3DAbstract Authors: Vuyisich M, Sanders CK, Graves SW Abstract Anthrax lethal toxin (LT) is a major virulence factor of Bacillus anthracis. The vast majority of the anthrax toxin-related literature describes the assembly of LT as a cell-dependent process. However, some reports have provided evidence for the existence of a fully assembled LT, either in vitro or in the bloodstream of anthrax-infected animals. To follow up on this work, we present studies on fully-assembled LT. We first demonstrate facile and cell-free assembly and purification of LT. We then show that fully assembled LT binds an anthrax toxin receptor with almost 100-fold higher affinity than the protective antigen (PA) alone. Quantitative cell intoxication assays were used to determine the LD(50) (lethal dose 50) for LT. The c... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576905 Thu, 05 Jan 2012 05:00:00 +0100 5576905 http://www.medworm.com/index.php?rid=5576904&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22219087%26dopt%3DAbstract Authors: Hussain R, Daud S, Kakar N, Ahmad A, Baloch AH, Tareen AM, Kakar MA, Ahmad J Abstract Canavan disease (OMIM 271900) is an autosomal recessive lethal neurodegenerative disorder characterized by spongy degeneration of the brain. A highly consanguineous Pakistani family with Canavan disease was enrolled on the basis of diagnosis. All the affected individuals have mental retardation, megalocephaly and degradation of motor skills, poor head control, partial vision loss, weakness of the muscles and raised urinary concentration of N-acetyl aspartic acid in the urine. Blood samples were collected from affected as well as normal siblings and processed for DNA purification. Linkage analysis was performed by typing three short tandem repeat markers D17S1583 (7.19 cM), D17S1828 (10.0... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576904 Thu, 05 Jan 2012 05:00:00 +0100 5576904 http://www.medworm.com/index.php?rid=5576909&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22215213%26dopt%3DAbstract Authors: Mukhopadhyay K, Haque I, Bandopadhyay R, Covert S, Porter D Abstract Despite the economical importance of shiitake (Lentinula ssp.) mushrooms, until the present date little information exists on cultivated and wild species in correlation with geographic origin applying molecular techniques. Use of a high resolution molecular tool like AFLP for assessing genetic similarity and geographical diversity would be an important step towards understanding of different Lentinula species. Thirteen wild and 17 cultivated accessions of 3 Lentinula species were analysed with 64 EcoRI-MseI primer combinations and finally 32 reproducible and polymorphic primer combinations were considered for the analysis. A total of 816 informative AFLP markers were generated and scored as binary data. T... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576909 Wed, 04 Jan 2012 05:00:00 +0100 5576909 http://www.medworm.com/index.php?rid=5576908&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22215214%26dopt%3DAbstract Authors: Zhang Y, Chen GQ, Ji Y, Huang B, Shen WS, Deng LC, Xi L, Cao XM Abstract Published studies on the relationships between 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms and lung cancer risk have been conflicting. To derive a more precise estimation of the relationship, a meta-analysis was performed. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the association between MTHFR C677T and A1298C polymorphisms and lung cancer risk. A total of 15 studies including 10,753 cases and 11,275 controls described C677T genotypes, among which 11 articles totalling 6,161 cases and 7,684 controls described A1298C genotypes, were also involved in this meta-analysis. Overall, no significantly elevated lung cancer risk was found in any genetic mo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576908 Wed, 04 Jan 2012 05:00:00 +0100 5576908 http://www.medworm.com/index.php?rid=5576912&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212708%26dopt%3DAbstract Authors: Du YJ, Hou YL, Hou WR Abstract ATP6V1F encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of ATP6V1F were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. The cDNA fragment cloned is 364 bp in size, containing an open reading frame of 360 bp encoding 119 amino acids. Alignment analysis indicated that both ORF and the deduced amino acid sequence are highly conserved. The length of the genomic sequence of the Giant Panda is 2225 bp, including two exons and one intron. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphoryla... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576912 Tue, 03 Jan 2012 05:00:00 +0100 5576912 http://www.medworm.com/index.php?rid=5576911&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212709%26dopt%3DAbstract Authors: Ye RS, Pan HB, Yin GF, Huang Y, Zhao SM, Gao SZ Abstract Three novel ovine genes were obtained from muscle full-length cDNA library of black-boned sheep. Sequence analysis revealed that nucleotide sequences of these genes were not homologous to any of the known sheep or goat genes, but these genes have high similarity to ATP synthase subunit O (ATP5O), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (NDUFA12) and ubiquinol-cytochrome c reductase hinge protein (UQCRH) genes of other mammal animals (accession number: FJ546085, FJ546078 and FJ546083). The alignment analysis showed that the ovine ATP5O, NDUFA12 and UQCRH genes and proteins have closer genetic relationships with the ATP5O, NDUFA12 and UQCRH genes and proteins from cattle. Conserved domain prediction show... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576911 Tue, 03 Jan 2012 05:00:00 +0100 5576911 http://www.medworm.com/index.php?rid=5576910&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212710%26dopt%3DAbstract In conclusion, altered expression and activity of HuR might participate in PDGF-induced ASM cells cyclinD1 expression and proliferation. The effectiveness of AMPK activation indicated a novel intervention method for airway remodeling. PMID: 22212710 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5576910 Tue, 03 Jan 2012 05:00:00 +0100 5576910 http://www.medworm.com/index.php?rid=5558392&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209985%26dopt%3DAbstract This study did not reveal any significant differences in genotype frequencies between healthy individuals and patients with head and neck cancer or with the clinical parameters. This study showed an increase frequency of the Q36R polymorphism in pharyngeal cancer. PMID: 22209985 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558392 Sun, 01 Jan 2012 05:00:00 +0100 5558392 http://www.medworm.com/index.php?rid=5558390&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209986%26dopt%3DAbstract In this study, the authors performed a meta-analysis to investigate the associations of +869T/C and +915G/C polymorphisms in TGF-β1 gene with hypertension risk in Chinese. Published literature from PubMed, EMBASE, CNKI, CBM, and Wanfang Data were searched. Pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using fixed or random-effects model. Nine studies (1,995 cases/1,840 controls) for +869T/C polymorphism and seven studies (1,547 cases/1,577 controls) for +915G/C polymorphism were included in the meta-analysis. The overall result showed that there was a statistically significant association between +869T/C polymorphism and hypertension risk (CC vs. TT: OR = 1.80, 95% CI 1.34-2.44). Similar results were found among two geographic locations and two subgroups with d... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558390 Sun, 01 Jan 2012 05:00:00 +0100 5558390 http://www.medworm.com/index.php?rid=5558410&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209950%26dopt%3DAbstract Authors: Yang L, Liu X, Huang J, Yang Q, Qiu L, Liu W, Jiang S Abstract MAPK kinase 1 interacting protein 1 (MAP2K1ip1) is an important scaffold proteins of the mitogen-activated protein kinase (MAPK) pathway that form an active signaling module and enhance the specificity and spatiality of MAPK signaling. In the present study, we identified and characterized a MAP2K1ip1 cDNA from tiger shrimp Penaeus monodon (designated as PmMAP2K1ip1). The open reading frame of PmMAP2K1ip1 is 372 bp encoding 123 amino-acid residues with a MAPK interaction domain. The predicted PmMAP2Kip1 protein is 13.6 KDa with the theoretical isoelectric point of 6.3. PmMAP2K1ip1 shared the highest amino acid with Nasonia vitripennis and Strongylocentrotus purpuratus, at 48% and 47.5%, respectively. Phylogeni... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558410 Sat, 31 Dec 2011 05:00:00 +0100 5558410 http://www.medworm.com/index.php?rid=5558409&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209951%26dopt%3DAbstract Authors: Chen T, Yang Q, Gruber M, Kang J, Sun Y, Ding W, Zhang T, Zhang X Abstract Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized from alfalfa. The MsERF8 cDNA has an open reading frame of 603 bp and encodes a nuclear protein of 201 amino acids. Q-RT-PCR analysis revealed that MsERF8 was strongly enriched in roots and leaves compared with stems, flower buds and flowers of mature alfalfa plants. Bioinformatic analysis of the MsERF8 promoter indicated a number of elements associated with stress-related responses, and MsERF8 transcripts in alfalfa seedlings were induced by NaCl, PEG6000, Al(2)(SO(4))(3) and five different h... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558409 Sat, 31 Dec 2011 05:00:00 +0100 5558409 http://www.medworm.com/index.php?rid=5558408&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209952%26dopt%3DAbstract In conclusion, +405 VEGF C/G SNP is associated with EH in patients with diabetes, suggesting presence of G allele and GG or CG genotype confer susceptibility towards EH. PMID: 22209952 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558408 Sat, 31 Dec 2011 05:00:00 +0100 5558408 http://www.medworm.com/index.php?rid=5558489&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207169%26dopt%3DAbstract Authors: Luo C, Zhong HJ, Zhu LM, Wu XG, Ying JE, Wang XH, Lü WX, Xu Q, Zhu YL, Huang J Abstract Anti-tumor activity and mechanism of matrine is evaluated and investigated. MTT assay showed that the matrine was able to inhibit gastric cancer cell line MNK45 in a dose-dependent manner. The concentration required for 50% inhibition (IC50) was found to be 540 μg/ml. This anti-tumor function was achieved through modulation of the NF-κB, XIAP, CIAP, and p-ERK proteins expression in cell line MNK45. By western blot analysis, we found that expression of NF-κB, XIAP, CIAP, and p-ERK proteins in cell line MNK45 would vary with varying concentration of matrine. These protein interactions possibly play a pivotal role in the regulation of apoptosis, for which further detailed analyzes are... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558489 Fri, 30 Dec 2011 05:00:00 +0100 5558489 http://www.medworm.com/index.php?rid=5558480&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207170%26dopt%3DAbstract Authors: Kitpipit T, Linacre A Abstract The complete mitochondrial genomes of five tiger samples from three subspecies (P. t. sumatrae, P. t. altica, and P. t. tigris) were successfully obtained by using 26 specifically designed Panthera-specific primer sets. The genome organization and gene arrangement of the five tiger samples were similar to each other; however polymorphic tandem repeat sequences were observed in the control region (CR). This led to a difference in the genome lengths obtained from these five samples with an average size of 16,994 bp for the five tiger mitochondrial genomes. The nucleotide base composition was on average as follows: A, 31.8%; T, 27.0%; C, 26.6%; G, 14.6% and exhibited compositional asymmetry. Most of tiger mitochondrial genome characteristics ar... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558480 Fri, 30 Dec 2011 05:00:00 +0100 5558480 http://www.medworm.com/index.php?rid=5558477&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207171%26dopt%3DAbstract Authors: Zhang B, Ding J, Li Y, Wang J, Zhao Y, Wang W, Shi S, Dong F, Zhang Z, Shi F, Xu Y Abstract G protein-coupled receptor 3 (Gpr3) is a member of G protein-coupled receptor rhodopsin family, which is present throughout the follicle within the ovary and functions as a critical factor for the maintenance of meiotic prophase arrest in oocytes by a Gs protein-mediated pathway. In the current paper, attempts were made to clone and characterize a gene encoding Gpr3 from pigs and investigate its expression pattern in tissues and the whole cumulus-oocyte complexes (COCs) in vitro maturation (IVM). Rapid amplification of cDNA ends and RT-PCR gave rise to the full sequence of Gpr3 gene with its length being 2101 bp nucleotides, including an open reading frame of 993 bp, encoding a 33... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558477 Fri, 30 Dec 2011 05:00:00 +0100 5558477 http://www.medworm.com/index.php?rid=5558471&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207172%26dopt%3DAbstract Authors: Dong CH, Li C, Yan XH, Huang SM, Huang JY, Wang LJ, Guo RX, Lu GY, Zhang XK, Fang XP, Wei WH Abstract Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and met... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558471 Fri, 30 Dec 2011 05:00:00 +0100 5558471 http://www.medworm.com/index.php?rid=5558470&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207173%26dopt%3DAbstract Authors: Liu C, Cao X, Zhang Y, Xu H, Zhang R, Wu Y, Lu P, Jin F Abstract The aim is to investigate the clinical implications of the Oct-4 and Nestin protein in human breast cancers. A total of 346 cases including 26 fresh and 320 paraffin-embedded tumor tissues were selected for characterizing the frequency of CD44(+)CD24(-) tumor cells by flow cytometry and the differential expression of the stem cell-related genes between CD44(+)CD24(-) and non-CD44(+)CD24(-) tumor cells was analyzed by PCR Array and immunofluorescence. In comparison with the non-CD44(+)CD24(-) tumor cells, the CD44(+)CD24(-), particularly for those with high percentage of Oct-4(+) and Nestin(+), tumor cells had higher tumorigenicity by forming mammospheres in vitro. More importantly, 42 (13.125%) out of 320 tum... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558470 Fri, 30 Dec 2011 05:00:00 +0100 5558470 http://www.medworm.com/index.php?rid=5558467&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207174%26dopt%3DAbstract Authors: Ong WD, Voo CL, Kumar SV Abstract Improving the quality of the non-climacteric fruit, pineapple, is possible with information on the expression of genes that occur during the process of fruit ripening. This can be made known though the generation of partial mRNA transcript sequences known as expressed sequence tags (ESTs). ESTs are useful not only for gene discovery but also function as a resource for the identification of molecular markers, such as simple sequence repeats (SSRs). This paper reports on firstly, the construction of a normalized library of the mature green pineapple fruit and secondly, the mining of EST-SSRs markers using the newly obtained pineapple ESTs as well as publically available pineapple ESTs deposited in GenBank. Sequencing of the clones from the E... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558467 Fri, 30 Dec 2011 05:00:00 +0100 5558467 http://www.medworm.com/index.php?rid=5558462&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207175%26dopt%3DAbstract Authors: Mohindra V, Singh A, Barman AS, Tripathi R, Sood N, Lal KK Abstract Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained, out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function and 1 for hypothetical protein. Nine ESTs with SSRs a... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558462 Fri, 30 Dec 2011 05:00:00 +0100 5558462 http://www.medworm.com/index.php?rid=5558461&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207176%26dopt%3DAbstract Authors: Cao G, Zhang Y, Xue R, Zhu Y, Wei Y, Zheng X, Gong C Abstract VASA is considered to be one of the most reliable molecular marker of germ cells. In order to study the Bombyx mori vasa-like gene (Bmvlg), the cDNAs of Bmvlg were cloned and sequenced, and the results showed that the Bmvlg gene from the fifth instar larval testes had four alternative splicing isoforms. The open reading frame (ORF) of the longest isoform was composed of 1,806 nucleotides encoding 601 amino acid residues and contained some known conserved domains. The other three isoforms had complete ORF, suggesting that the Bmvlg gene had several alternative splicing forms, completely different from that of Drosophila melanogaster. The results of sequencing demonstrated that the Bmvlg gene p... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558461 Fri, 30 Dec 2011 05:00:00 +0100 5558461 http://www.medworm.com/index.php?rid=5558460&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207177%26dopt%3DAbstract In this study, we investigated the role of GSTM1, GSTT1, GSTP1 exon 5 and exon 6 polymorphisms on developing lung cancer and histological subtypes in 213 lung cancer patients and 231 controls. GSTM1 null, GSTT1 null, and GSTP1 exon 5 variant genotypes did not show a significant risk for developing lung cancer overall. Significant association was noted between GSTP1 exon 6 variant genotypes and overall lung cancer risk (OR 2.17, 95% CI 1.25-3.78; P = 0.006). These results show that GSTP1 exon 6 polymorphism might be an important factor in determining lung cancer susceptibility in a Turkish population. PMID: 22207177 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558460 Fri, 30 Dec 2011 05:00:00 +0100 5558460 http://www.medworm.com/index.php?rid=5558459&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207178%26dopt%3DAbstract Authors: Rai H, Fitt J, Sharma AK, Sinha N, Kumar S, Pandey CM, Agrawal S, Mastana S Abstract Nitric Oxide (NO) is an important molecule carrying number of different functions in humans. Published studies suggest that it may inhibit several key steps involved in the pathogenesis of atherosclerosis. Inhibition or reduction of NO due to Glu298Asp polymorphism may accelerate atherosclerosis. The aim of this study was to determine whether Glu298Asp polymorphism is implicated in the pathogenesis of coronary artery disease (CAD) among North Indian population from the state of Uttar Pradesh, India. We selected 253 CAD patients and 174 healthy, normotensive, non-diabetic controls, which were matched for gender and ethnicity. The Glu298Asp (rs1799983) variant was detected by genotyping subj... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558459 Fri, 30 Dec 2011 05:00:00 +0100 5558459 http://www.medworm.com/index.php?rid=5558458&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207179%26dopt%3DAbstract This study will enrich the information in understanding the innate immune mechanism in fish, and will be helpful in developing preventive measures against infectious diseases in fish. PMID: 22207179 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558458 Fri, 30 Dec 2011 05:00:00 +0100 5558458 http://www.medworm.com/index.php?rid=5558450&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207180%26dopt%3DAbstract Authors: Zhang WB, Qiu PC, Jiang HW, Liu CY, Xin DW, Li CD, Hu GH, Chen QS Abstract Northeast of China is the main soybean production area, drought and low-temperature tolerance are both main factors involved in reducing soybean yield and limiting planting regions, the most effective way to solve this problem is to breed cultivars with drought and low-temperature tolerance. A set of the BC(2)F(3) lines was constructed with Hongfeng 11 as recurrent parent and Harosoy as donor parent, and screened in drought and low-temperature condition at the germination stage. Related QTLs were obtained by Chi-test and ANOVA analysis with genotypic and phenotypic data. Eighteen QTLs of drought tolerance and 23 QTLs of low-temperature tolerance were detected. Among them, 12 QTLs were correlated wit... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558450 Fri, 30 Dec 2011 05:00:00 +0100 5558450 http://www.medworm.com/index.php?rid=5558439&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207181%26dopt%3DAbstract Authors: Naghibalhossaini F, Zamani M, Mokarram P, Khalili I, Rasti M, Mostafavi-Pour Z Abstract The WNT signaling is deregulated in most human colorectal cancers (CRC). Promoter methylation has been proposed as an alternative mechanism to inactivate genes in tumors. To gain insight into the methylation silencing of the WNT pathway during colorectal carcinogenesis, we examined the aberrant methylation profile of four genes, APC, Axin1, Axin2, and GSK3β in an unselected series of 112 sporadic colorectal tumors by methylation specific PCR. It has been suggested that the Axin2 C148T SNP is associated with the risk of developing certain types of cancers. To assess the contribution of Axin2 SNP to CRC susceptibility, we examined the Axin2 C148T genotype in CRC patients and 170 healthy ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558439 Fri, 30 Dec 2011 05:00:00 +0100 5558439 http://www.medworm.com/index.php?rid=5558415&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207182%26dopt%3DAbstract This study was designed to assess chromosomal status with metaphase comparative genomic hybridization after in vitro maturation (IVM) in unstimulated cycles and compare the results with those obtained after in vivo maturation. Patients without any obstetrical or gynecological pathology were admitted into the study. IVM oocytes were collected 36 h post hCG and matured in vitro at 37°C in 5% O(2), 6% CO(2), and 89% air for 36 h. All matured (metaphase II) oocytes were subject to polar body 1 (PB-1) biopsy and vitrified individually. PB-1 samples were transferred into 0.25 cc PCR tubes containing 2.5 μl of PBS. PB-1 samples from 12 IVM patients were studied. Twenty-six out of 63 PB-1 samples (41%) were determined as euploid and 37 samples (59%) were aneuploid, whereas these values were... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558415 Fri, 30 Dec 2011 05:00:00 +0100 5558415 http://www.medworm.com/index.php?rid=5558411&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207183%26dopt%3DAbstract In this study, we retrospectively reviewed the records of 1,152 FMF suspected patients (673 female and 479 male) from November 2006 to December 2010. A commercial kit assay for the identification of MEFV (Mediterranean fever) gene mutations based on PCR and reverse-hybridization was used to investigate 12 mutations of the MEFV gene. 52.17% of 1,152 FMF suspected patients had MEFV mutation and 45.25% of them were male. The rate of MEFV mutation among male and female patients were 56.78 and 48.88%, respectively. These results were statistically significant and might support the suggestion that FMF had much more penetrance in male patients (P = 0.009). Not any significant difference was observed between the male and female patients in terms of heterozygote and homozygote mutation carriage r... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558411 Fri, 30 Dec 2011 05:00:00 +0100 5558411 http://www.medworm.com/index.php?rid=5558511&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205537%26dopt%3DAbstract Authors: Long G, Song J, Deng Z, Liu J, Rao L Abstract A proteomic approach was employed to investigate the cold stress-responsive proteins in trifoliate orange (Poncirus trifoliata (L.) Raf.), which is a well-known cold tolerant citrus relative and widely used as rootstock in China. Two-year-old potted seedlings were exposed to freezing temperature (-6°C) for 50 min (nonlethal) and 80 min (lethal), and the total proteins were isolated from leaves of the treated plants. Nine differentially accumulated proteins over 2-fold changes in abundance were identified by two-dimensional gel electrophoresis and mass spectrometry. Among these proteins, a resistance protein induced by the nonlethal cold treatment (protein spot #2 from P. trifoliata) was selected as target sequence for degene... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558511 Thu, 29 Dec 2011 05:00:00 +0100 5558511 http://www.medworm.com/index.php?rid=5558510&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205538%26dopt%3DAbstract Authors: Bayram S, Akkız H, Bekar A, Akgöllü E, Yıldırım S Abstract Exonuclease 1 (Exo 1) is an important nuclease involved in mismatch repair system that contributes to maintain genomic stability, to modulate DNA recombination, and to mediate cell cycle arrest. A guanine (G)/adenine (A) common single nucleotide polymorphism at first position of codon 589 in Exo 1 gene determines a glutamic acid (Glu, E) to lysine (Lys, K) (K589E) aminoacidic substitution which may alter cancer risk by influencing the activity of Exo 1 protein. Exo 1 K589E polymorphism has been studied in various cancers, but its association with hepatocellular carcinoma (HCC) has yet to be investigated. To determine the association of the Exo 1 K589E polymorphism with the risk of HCC development in a Turkish... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558510 Thu, 29 Dec 2011 05:00:00 +0100 5558510 http://www.medworm.com/index.php?rid=5558509&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205539%26dopt%3DAbstract Authors: Xiang ZL, Zeng ZC, Fan J, Tang ZY, Zeng HY Abstract In the present study, we evaluated the prognostic value of intratumoral and peritumoral expression of connective tissue growth factor (CTGF), transforming growth factor-beta 1 (TGF-β1), and interleukin-11 (IL-11) in patients with hepatocellular carcinoma (HCC) after curative resection. Expression of CTGF, TGF-β1, and IL-11 was assessed by immunohistochemical staining of tissue microarrays containing paired tumor and peritumoral liver tissue from 290 patients who had undergone hepatectomy for histologically proven HCC. The prognostic value of these and other clinicopathologic factors were evaluated. The median follow-up time was 54.3 months (range, 4.3-118.3 months). High intratumoral CTGF expression was associated wit... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558509 Thu, 29 Dec 2011 05:00:00 +0100 5558509 http://www.medworm.com/index.php?rid=5558496&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205540%26dopt%3DAbstract This study aims to evaluate the effect of mechanical stretch on the differentiation of BMSCs with a co-culture with pelvic ligament fibroblasts. BMSCs was isolated and identified from 7 day SPF SD rats. Rat pelvic ligament fibroblasts were obtained from rat pelvic ligament. The fourth passage of fibroblasts was subjected to 10% deformation with 1 Hz, 12 h periodic one-way mechanical stretch stimulation, and the cells were then co-cultured with BMSCs. The longer co-culture and co-culture with mechanical stretch (i.e. 6 and 12 days) induced more expression of elastin, LOX, and Fibulin-5, compared to the groups without stimulation. Compared to co-culture group each, Co-culture with mechanical stretch stimulation group induced significant expression of elastin, LOX, and Fibulin-5, both in ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558496 Thu, 29 Dec 2011 05:00:00 +0100 5558496 http://www.medworm.com/index.php?rid=5558490&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205541%26dopt%3DAbstract Authors: Shi KQ, Fan YC, Ying L, Lin XF, Song M, Li LF, Yu XY, Chen YP, Zheng MH Abstract Risk stratification for spontaneous bacterial peritonitis (SBP) in patients with cirrhosis and ascites helps guide care. Existing prediction models, such as end-stage liver disease (MELD) score, are accurate but controversial in clinical practice. We developed and validated a practical user-friendly bedside tool for SBP risk stratification of patients with cirrhosis and ascites. Using classification and regression tree (CART) analysis, a model was developed for prediction of SBP in cirrhosis with ascites. The CART model was derived on data collected from 676 patients admitted from January 2007 to December 2009 retrospectively, and then was prospectively tested in another independent 198 inpati... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558490 Thu, 29 Dec 2011 05:00:00 +0100 5558490 http://www.medworm.com/index.php?rid=5558602&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203478%26dopt%3DAbstract Authors: Xu JW, Zhao W, Xu YN, Zhong JJ PMID: 22203478 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558602 Wed, 28 Dec 2011 05:00:00 +0100 5558602 http://www.medworm.com/index.php?rid=5558599&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203480%26dopt%3DAbstract Authors: Liu Q, Zheng J, Yin DD, Xiang J, He F, Wang YC, Liang L, Qin HY, Liu L, Liang YM, Han H Abstract Macrophage activation is modulated by both environmental cues and endogenous programs. In the present study, we investigated the role of a PAQR family protein, monocyte to macrophage differentiation-associated (MMD), in macrophage activation and unveiled its underlying molecular mechanism. Our results showed that while MMD expression could be detected in all tissues examined, its expression level is significantly up-regulated upon monocyte differentiation. Within cells, EGFP-MMD fusion protein could be co-localized to endoplasmic reticulum, mitochondria, Golgi apparatus, but not lysosomes and cytoplasm. MMD expression is up-regulated in macrophages after LPS stimulation, and th... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558599 Wed, 28 Dec 2011 05:00:00 +0100 5558599 http://www.medworm.com/index.php?rid=5558596&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203481%26dopt%3DAbstract In conclusion, ATM rs664143 polymorphism was associated with cancer susceptibility. ATM rs664143 polymorphism was significantly associated with lung cancer risk. ATM rs664677 polymorphism was associated with decreased lung cancer risk as well as increased breast cancer risk. PMID: 22203481 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558596 Wed, 28 Dec 2011 05:00:00 +0100 5558596 http://www.medworm.com/index.php?rid=5558591&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203482%26dopt%3DAbstract This study provides useful information for further study of SmGGPPs and its regulator effect on the biosynthetic process of tanshinones so that researchers can improve the tanshinone contents in S. miltiorrhiza. PMID: 22203482 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558591 Wed, 28 Dec 2011 05:00:00 +0100 5558591 http://www.medworm.com/index.php?rid=5558590&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203483%26dopt%3DAbstract Authors: Niu JZ, Dou W, Ding TB, Yang LH, Shen GM, Wang JJ Abstract Quantitative real time reverse transcriptase polymerase chain reaction (RT-qPCR) is preferred for gene expression analysis in living organisms. Currently, it is a valuable tool for biological and ecological studies as it provides a relatively straightforward way to assess the relevance of transcriptional regulation under developmental and stress tolerance conditions. However, studies have shown that some commonly used reference genes varied among different experimental treatments, thus, systematic evaluation of reference genes is critical for gene expression profiling, which is often neglected in gene expression studies of arthropods. The aim of this study is to identify the suitable reference genes for RT-qPCR exp... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558590 Wed, 28 Dec 2011 05:00:00 +0100 5558590 http://www.medworm.com/index.php?rid=5558559&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203484%26dopt%3DAbstract Authors: Mostowska M, Lianeri M, Oko A, Mostowska A, Jagodziński PP Abstract Various studies have indicated that chemokines such as monocyte chemotactic protein-1 (MCP-1) play an important role in the pathogenesis of primary glomerulonephritis (GN) and other glomerular diseases. Moreover, patients with primary GN display aberrant galactosylation of the O-linked carbohydrate moieties of IgA. Therefore, we analysed the distribution of the functional MCP-1 -2518 A > G (rs 1024611) and 1 beta 1,3-galactosyltransferase (C1GalT1) 1365 A > G (rs1047763) polymorphic variants in patients with primary GN (n = 144) and controls (n = 437) in a sample of the Polish population. We did not find a significant difference in the prevalence of the MCP-1 -2518 A > G and C1GalT1 136... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558559 Wed, 28 Dec 2011 05:00:00 +0100 5558559 http://www.medworm.com/index.php?rid=5558538&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203485%26dopt%3DAbstract Authors: Zheng LP, Hou L, Yu M, Li X, Zou XY Abstract Spätzle gene codes for a NGF-like protein, it involves in the embryonic development and innate immune response of insects and other invertebrate. In dorsal ventral axis differentiation, proSpätzle is activated by serine endoproteases Easter and then binds to the Toll receptor in ventral axis of oocyte which initiates the ventral axis development. Besides, it could also be activated by another protease named Spätzle-processing enzyme (SPZ) to mediate Toll pathway which involves in innate immune response in fungal and Gram-positive bacterial infection of invertebrate. In this paper, a full-length cDNA of Spätzle was firstly isolated from Artemia sinica which belonged to Spätzle-4 family. The expression of Spätzle was investi... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558538 Wed, 28 Dec 2011 05:00:00 +0100 5558538 http://www.medworm.com/index.php?rid=5558537&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203487%26dopt%3DAbstract This study was scheduled to determine whether the presence of JAK2V617F mutation in thalassemic patients is associated with thrombocytosis. We studied 20 patients DNA with beta-thalassemia for JAK2V617F mutation by using RG-PCR method. None of the patients were positive for this particular mutation. More studies are needed to prove the role of JAK2 in ineffective erythropoiesis, iron metabolism and thrombocytosis and to determine if using JAK2 inhibitors in thalassemic patients can be a potential therapeutic option. PMID: 22203487 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558537 Wed, 28 Dec 2011 05:00:00 +0100 5558537 http://www.medworm.com/index.php?rid=5558536&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203488%26dopt%3DAbstract In this report we describe the construction of a vector for TA-cloning, based on the abolishment of the Eam1105I recognition site within the β-lactamase gene by site-directed mutagenesis, and the introduction of a stuffer flanked by Eam1105I target sites within the polylinker of the pBluescript SK+ plasmid. PMID: 22203488 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558536 Wed, 28 Dec 2011 05:00:00 +0100 5558536 http://www.medworm.com/index.php?rid=5558535&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203489%26dopt%3DAbstract In conclusion, it is possible to determine a specific family history in young adults with CVD. From this perspective, the emergence of more serious CVD may be prevented by providing disease-related information to the other family members and implementing preventive measures. PMID: 22203489 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558535 Wed, 28 Dec 2011 05:00:00 +0100 5558535 http://www.medworm.com/index.php?rid=5558524&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203490%26dopt%3DAbstract This study investigated the role of the mevalonate pyrophosphate decarboxylase gene in the triterpene biosynthetic pathway of Ganoderma lucidum. The mevalonate pyrophosphate decarboxylase gene (mvd) was isolated using a degenerate primer-PCR technique. An analysis of the Gl-mvd transcription profile revealed a positive correlation between the expression of the Gl-mvd gene and triterpene content changes in G. lucidum during development. Furthermore, a promoter deletion analysis was conducted in G. lucidum to investigate the promoter activity and the role of methyl jasmonate (MeJA) responsive elements in the mvd promoter under the MeJA elicitor. The overexpression of Gl-mvd increased triterpene accumulation compared with the wild-type strain and increased the expression of several genes invo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558524 Wed, 28 Dec 2011 05:00:00 +0100 5558524 http://www.medworm.com/index.php?rid=5558513&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203491%26dopt%3DAbstract Authors: Liu S, Tao Y, Chen X, Cao Y Abstract The dynamic interplay in polycomb group (PcG) and trithorax group (TrxG) proteins in response to DNA damage directly involves in the DNA double strand breaks (DSBs) sites and potentially function in both homologous recombination (HR) and nonhomologous end joining (NHEJ) pathways. The process includes chromatin remodeling that is a major mechanism used by cells to relax chromatin in DNA damage response (DDR) and repair. PcGs show resistance ability to the process while, some tumor suppressor genes involves in the DDR and repair by interacting with TrxGs. Understanding how the dynamic interplay in PcGs and TrxGs impacts on DDR will shed light on the mechanisms of carcinogenesis and develop a new target from anti-DDR related drugs. PMI... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5558513 Wed, 28 Dec 2011 05:00:00 +0100 5558513 http://www.medworm.com/index.php?rid=5545324&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201020%26dopt%3DAbstract Authors: Chen G, Mi J, Xiao MZ, Fu YR Abstract Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate possible mechanisms, we describe a comparison between differentially proteins expression and immune markers profile (IL-2, IL-4, IL-6, and CRP) of acute rejection and the controls. Through quantitative real-time RT-PCR confirmation, PDIA3 mRNA and protein expression levels in serum and transplanted kidney in experiment group was significantly (P < 0.05) higher than that in control group. Immunity analysis showed that pla... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545324 Tue, 27 Dec 2011 05:00:00 +0100 5545324 http://www.medworm.com/index.php?rid=5545323&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201021%26dopt%3DAbstract Authors: McLaughlin RW, Chen M, Zheng J, Zhao Q, Wang D Abstract The aim of this study was to determine the bacteria present in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis. Fecal samples were collected from 12 Yangtze finless porpoises living in the wild at Poyang Lake, located in Jiangxi Province, China. To determine the bacterial diversity, a 16S rRNA gene clone library using the bacterial PCR primers fD1 and rP2, was prepared. A total of 138 near-full-length sequences were analyzed and 39 operational taxonomic units (OTUs) were identified. Sequences showing ≥97% similarity were grouped together as an OTU. Six different phyla were identified in which 38 OTUs were classified. Most of the OTUs contained sequences belonge... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545323 Tue, 27 Dec 2011 05:00:00 +0100 5545323 http://www.medworm.com/index.php?rid=5545322&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201022%26dopt%3DAbstract Authors: Liu ZW, Yin HX, Yi XP, Zhang AL, Luo JX, Zhang TY, Fu CY, Zhang ZH, Shen JC, Chen LP Abstract α-amy gene amplified from barley genome was cloned into MCS of pGAP9K to generate pGAP9K-α-amy which was then transformed into Pichia pastoris GS115 by electroporation. Transformants with multi-copies and high expression for the foreign gene were selected on G418 containing plate and expression analysis. The fermentation was carried out in a 50 l bioreactor with 20 l working volume, using a high-density cell culture method by continuously feeding with 50% glycerol-0.8% PTM4 to the growing culture for 54 h at 30°C. Under the control of GAP promoter (pGAP), α-amy gene was constitutively expressed. At the end of the fermentation, the α-AMY expression reached 125 mg/l, while ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545322 Tue, 27 Dec 2011 05:00:00 +0100 5545322 http://www.medworm.com/index.php?rid=5545321&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201023%26dopt%3DAbstract Authors: Hur YJ, Yi YB, Lee JH, Chung YS, Jung HW, Yun DJ, Kim KM, Park DS, Kim DH Abstract The ubiquitin-26S proteasome system is important in the quality control of intracellular proteins. The ubiquitin-26S proteasome system includes the E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligase) enzymes. U-box proteins are a derived version of RING-finger domains, which have E3 enzyme activity. Here, we present the isolation of a novel U-box protein, U-box containing E3 ligase induced by phosphate starvation (OsUPS), from rice (Oryza sativa). The cDNA encoding the O. sativa U-box protein (OsUPS) comprises 1338 bp, with an open reading frame of 445 amino acids. The amino acid sequence of OsUPS cDNA shows 41-79% identity with other plant U-box homologous gene... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545321 Tue, 27 Dec 2011 05:00:00 +0100 5545321 http://www.medworm.com/index.php?rid=5545320&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201024%26dopt%3DAbstract This study reported the identification and validation of nine reference genes, including β-tubulin (β-TUB), elongation factor 1 alpha (EF-1α), elongation factor 1 beta (EF-1β), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin (UBQ), actin 1/2(ACT-1 and ACT-2), 18S rRNA, and 26S rRNA, from Anoectochilus roxburghii (Wall.) Lindl., a valuable herb remedy widely used for various diseases treatment in traditional Chinese medicine. Transcriptional levels of the candidate reference genes were examined using qRT-PCR analysis and revealed differential expression of the genes in the leaf, stem, root, flower, and peduncle tissues. The relative quantities data were subjected to geNorm software for ranking the expression stability of the reference genes and the results showed that EF-1β... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545320 Tue, 27 Dec 2011 05:00:00 +0100 5545320 http://www.medworm.com/index.php?rid=5545319&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201025%26dopt%3DAbstract Authors: Pasaje CF, Bae JS, Park BL, Kim JH, Cheong HS, Uh ST, Park CS, Shin HD Abstract Modest effects of genes in various pathways are significant in the etiology of complex human diseases, including aspirin exacerbated respiratory disease (AERD). By functioning as a relevant component of respiratory processes, the human kinesin family member C1 (KIFC1) is hypothesized to play a role in AERD pathogenesis. A case-control analysis was carried out by comparing the genotype distribution of six KIFC1 single-nucleotide polymorphisms between 93 AERD cases and 96 aspirin-tolerant asthma controls in a Korean population. After controlling for confounds, logistic and regression models via various modes of genetic inheritance facilitated the association analysis. Initial results revealed sig... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545319 Tue, 27 Dec 2011 05:00:00 +0100 5545319 http://www.medworm.com/index.php?rid=5545318&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201026%26dopt%3DAbstract Authors: Lee YH, Choi SJ, Ji JD, Song GG Abstract The aim of this study was to identify candidate causal single nucleotide polymorphisms (SNPs) and candidate causal mechanisms of psoriasis and Behcets's disease (BD) and to generate an SNP → gene → pathway hypothesis. A psoriasis genome-wide association study (GWAS) dataset that included 436,192 SNPs in 1,409 psoriasis cases and 1,436 controls of European descent and a BD GWAS dataset that contained 310,324 SNPs in 1,215 BD cases and 1,278 controls were used in this study. Identify candidate causal SNPs and pathways (ICSNPathway) analysis was applied to the GWAS datasets. ICSNPathway analysis identified 15 candidate causal SNPs and 28 candidate causal pathways. The top five candidate causal SNPs were rs1063478 (P = 1.45E-1... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545318 Tue, 27 Dec 2011 05:00:00 +0100 5545318 http://www.medworm.com/index.php?rid=5545317&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22201027%26dopt%3DAbstract Authors: Gu H, Qiu W, Wan Y, Ding G, Tang W, Liu C, Shi Y, Chen Y, Chen S Abstract Growing evidence suggests that the checkpoint kinase 2 (CHEK2) signaling pathway occupies a central position in the signaling networks of DNA-damage signaling. Many functional and molecular epidemiological studies have evaluated the association between genetic variants of CHEK2 and various cancers. To evaluate the relationship between CHEK2 functional genetic variants and esophageal cancer risk and the risk of lymph node metastasis among a Chinese population. We genotyped CHEK2 rs738722, rs2236141 and rs2236142 single nucleotide polymorphisms (SNPs) using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry assay in a case-controlled study, including 380 esophageal cancer ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545317 Tue, 27 Dec 2011 05:00:00 +0100 5545317 http://www.medworm.com/index.php?rid=5545325&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22198626%26dopt%3DAbstract Authors: Koç Öztürk L, Ulucan K, Akyüz S, Furuncuoğlu H, Bayer H, Yarat A Abstract The aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545325 Sun, 25 Dec 2011 05:00:00 +0100 5545325 http://www.medworm.com/index.php?rid=5545327&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22198549%26dopt%3DAbstract Authors: Valdiani A, Kadir MA, Tan SG, Talei D, Abdullah MP, Nikzad S Abstract Nain-e Havandi (Andrographis paniculata Nees.) (AP) is an annual herbaceous plant belonging to the family Acanthacea. Only a few species of Andrographis genus out of 28 are medicinally concerned of which AP is the most important. Knowledge about the arrival of AP to Iran is extremely lacking but most probably it has been imported from India. However, evidence implies the familiarity of Iran's folkloric medicine with this plant, but it has been disappeared from contemporary medicine for unknown reasons. Presence of active ingredients from diterpenoids group such as andrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide has given incredible unique medicinal properties to the plant.... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545327 Sat, 24 Dec 2011 05:00:00 +0100 5545327 http://www.medworm.com/index.php?rid=5545326&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22198550%26dopt%3DAbstract Authors: Li N, Wang X, Zhang Y, Zhai J, Zhang T, Wei K Abstract In the present study, we investigated the differentially expressed proteins associated with ulcerative colitis (UC) using proteomic methods. Two-dimensional electrophoresis (2-DE) technology was performed to separate the total proteins of ulcerative tissues from those of the normal tissues of UC patients. PDQuest software was applied to analyze the obtained 2-DE images. Candidate protein spots between the two groups were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics analysis. The well resolution and reproducible 2-DE patterns of UC and normal tissues were established. Of the 12 differentially expressed proteins, 9 were successfully identified, of which ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545326 Sat, 24 Dec 2011 05:00:00 +0100 5545326 http://www.medworm.com/index.php?rid=5545337&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189541%26dopt%3DAbstract Authors: Gupta R, Xu SY, Sharma P, Capalash N Abstract MspNI and MspNII, isoschizomers of prototype Type II restriction endonucleases AvaII and BstYI, were extracted from an extreme thermophile bacterium belonging to the genus Meiothermus, isolated from the hot sulphur springs in north Himalayan region of India where temperature and pH ranged from 60 to 80°C and 7.5 to 8.5, respectively. The two enzymes were purified to homogeneity using Cibacron-Blue 3GA Agarose, Q-Sepharose and SP-Sepharose chromatography and were homodimers with subunit molecular weights of 27 and 45 kDa, respectively. Restriction mapping and run-off sequencing of MspNI and MspNII cleaved pBR322 DNA showed that they recognized and cleaved 5'-G/GWCC-3' and 5'-R/GATCY-3' sites, respectively. MspNI and MspNII wor... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545337 Fri, 23 Dec 2011 05:00:00 +0100 5545337 http://www.medworm.com/index.php?rid=5545332&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193622%26dopt%3DAbstract Authors: Guo X, Liu X, Xu X, Wu M, Zhang X, Li Q, Liu W, Zhang Y, Wang Y, Yu Y Abstract To identify the effects of the expression levels of DNMT3a and DNMT3b, coding the de novo methyltransferases DNMT3a and DNMT3b, on 16 beef carcass and quality traits, 50 beef cattle liver and ribeye muscle tissue samples were collected. Quantitative real-time RT-PCR was employed to quantify the expression level of these two genes, and a basic model included fixed effects of gender, age, and expression level of these two genes was used to analyze live weight; and slaughtering batches and aging days were added when beef carcass traits and beef quality traits were analyzed, respectively. Results showed that transcripts of DNMT3a and DNMT3b were present at significantly higher levels in liver tissue... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545332 Fri, 23 Dec 2011 05:00:00 +0100 5545332 http://www.medworm.com/index.php?rid=5545331&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193623%26dopt%3DAbstract In this study, we examined whether the susceptibility to AIS is associated with MATN1 gene polymorphisms in a Korean population, which included 166 individuals with AIS and 126 controls. We found that there were no statistically significant associations between any of the MATN1-linked allele or genotype frequencies between AIS and controls. However, statistically significant associations were found at single nucleotide polymorphism (SNP) rs1065755 when comparing the curve patterns of AIS with the controls. The A allele of SNP rs1065755 was associated with a higher risk of AIS than the allele G in the genotype-phenotype (curve pattern) analysis (P = 0.029). In addition, the frequency of the A allele of SNP rs1065755 in AIS with double major curves was higher than in controls (P = 0.021,... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545331 Fri, 23 Dec 2011 05:00:00 +0100 5545331 http://www.medworm.com/index.php?rid=5545330&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193624%26dopt%3DAbstract Authors: Jaiswal PK, Goel A, Mandhani A, Mittal RD Abstract Survivin is a member of novel inhibitor of apoptosis protein family which expressed in human cancers. The molecular detection of bladder cancer by targeting Survivin as a novel marker may be useful in the occurrence and progression of cancer. We genotyped Survivin -31G>C, -1547A>G and -241C>T by PCR-restriction fragment length polymorphism to evaluate the risk of bladder cancer (BC) in 200 BC patients and 200 healthy controls from North Indian cohort. We observed significant increased BC risk associated with variant CC genotype of Survivin -31G>C having 2.6 fold risk. The variant genotype of Survivin -1547A>G was significantly associated with BC risk (P = 0.047). In case of Survivin -241C>T the protecti... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545330 Fri, 23 Dec 2011 05:00:00 +0100 5545330 http://www.medworm.com/index.php?rid=5545329&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193625%26dopt%3DAbstract In this study, we performed a meta-analysis to evaluate the association between the VDR BsmI (rs1544410) and ApaI (rs7975232) polymorphisms and BMD in Chinese women. Literature was retrieved from PubMed and other databases. The studies on the association between VDR BsmI and ApaI genotypes and BMD at the lumbar spine, the femoral neck, the trochanter or the Ward's triangle in Han Chinese women were included in this meta-analysis. Pooled BMD differences and 95% confidence intervals (CIs) were calculated using random- or fixed- effects model. Twenty-five eligible studies, which included 4,075 Chinese women, were identified. No significant difference was observed for either genotype when the meta-analysis was limited to premenopausal women. In postmenopausal women, BMD dif... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545329 Fri, 23 Dec 2011 05:00:00 +0100 5545329 http://www.medworm.com/index.php?rid=5545328&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193626%26dopt%3DAbstract Authors: Farquharson AJ, Steele RJ, Carey FA, Drew JE Abstract The role of altered levels of insulin, leptin and adiponectin in contributing to the observed increased risk of colon cancer associated with obesity remains to be determined. Elevated insulin and leptin associated with obesity are linked to inflammatory responses. Conversely, adiponectin levels are reduced in obese individuals and this hormone is generally associated with anti-inflammatory responses. Inflammatory cytokines are key components of processes linked with carcinogenesis. Insulin, leptin and adiponectin receptor expression profiles were assessed in human normal, adenomatous polyp and tumour tissue. Insulin, leptin and adiponectin regulation of inflammatory cytokines previously identified as being associated wi... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545328 Fri, 23 Dec 2011 05:00:00 +0100 5545328 http://www.medworm.com/index.php?rid=5545340&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189538%26dopt%3DAbstract In this study, we investigated whether genetic polymorphisms of the Interferon gamma (IFNG) gene were associated with the number of diseased vessels in CAD patients in the Korean population. To observe the association between the IFNG gene and the number of diseased vessels, we genotyped 635 CAD patients for a single nucleotide polymorphism (SNP, rs2430561) and a microsatellite (CA(n) repeats, rs3138557) located in the first intron of the IFNG gene using the direct sequencing and gene scan method. The number of microsatellites was increased in the one- and two-vessel disease groups. A combined analysis of the genotype of rs2430561 and the number of microsatellites revealed that the number of diseased vessels was associated with CA(12)-TT and CA(13)-TT. Our results suggest that the IFNG gen... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545340 Thu, 22 Dec 2011 05:00:00 +0100 5545340 http://www.medworm.com/index.php?rid=5545339&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189539%26dopt%3DAbstract Authors: Sun QW, Jiang SM, Yang K, Zheng JM, Zhang L, Xu WD Abstract Activated rheumatoid arthritis (RA) fibroblast-like synoviocytes (RAFLSs) play a central role in both initiating and driving RA. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been documented to induce apoptosis only in a small proportion of RAFLSs, which is followed by an induction of proliferation in surviving cells. Apigenin, a chemopreventive bioflavonoid, exhibits proapoptotic activity in many types of cells. In the present study, we sought to determine whether apigenin could enhance the cytotoxic effect of TRAIL on activated RAFLSs. Human RAFLSs isolated from patients with RA were treated with TRAIL (1 nM), apigenin (20 μM), or their combination, and subjected to apoptosis analysis af... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545339 Thu, 22 Dec 2011 05:00:00 +0100 5545339 http://www.medworm.com/index.php?rid=5545338&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189540%26dopt%3DAbstract Authors: Shao Z, Shen J, Yang Y, Wu D, Zhou X, Yuan H Abstract To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by western blot and RT-PCR. Effects of varying concentrations of the Annexin 1 peptide fragment, Ac2-26, on the survival of the RGC-5 cells was determined, and apoptotic cells were quantified by flow cytometry. Immunoblot and RT-PCR analysis was preformed to identify caspase 3, bax and bcl-2 in RGC extracts. Annexin 1 was localized in the cytoplasm of RGC-5 cells and the expression of Annexin 1, caspase 3 an... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545338 Thu, 22 Dec 2011 05:00:00 +0100 5545338 http://www.medworm.com/index.php?rid=5545336&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189542%26dopt%3DAbstract This study aims to investigate the methylation level of Akt1 in bladder cancer tissues and their clinical significance. Methylation of the oncogene Akt1 transcriptional regulation region (TRR) was detected using bisulfite-specific (BSP) PCR-based sequencing analysis in cases of bladder cancer and the normal tissues, including 15 bladder cancer tissues, and five normal bladder tissues. BSP cloning-based sequencing analysis was also performed in selected cases. Clinicopathological data from the cancer patients were collected and analyzed. Analysis of Akt1 gene TRR methylation showed decreased methylation level in bladder cancer than normal. Methylation level of Akt1 has clinical relevance (P = 0.0043 by unpaired student's t test) with bladder cancer. Abnormal methylation of the Akt1 gene m... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545336 Thu, 22 Dec 2011 05:00:00 +0100 5545336 http://www.medworm.com/index.php?rid=5545335&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189543%26dopt%3DAbstract In this study, we concluded that the FTO gene may play a critical role in leading to MetS. Targeting this gene may provide novel therapeutic strategies for the prevention and treatment of metabolic syndrome. PMID: 22189543 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545335 Thu, 22 Dec 2011 05:00:00 +0100 5545335 http://www.medworm.com/index.php?rid=5545334&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189544%26dopt%3DAbstract In this study, a total of 309 unrelated individuals (200 males and 109 females) from Shanghai Han population were successfully analyzed with this kit. Hardy-Weinberg equilibrium tests demonstrated no significant deviation from expected values (P > 0.05) for all of the 12 X-STR loci in the Shanghai Han population. Linkage disequilibrium tests were performed for all pairs of loci by the Arlequin v3.1 software and only DXS10103-DXS10101 remained significant after adjustment for multiple testing (P < 0.05/66). The combined power of discrimination in males (CDP(M)) was 0.999999996 while in females (CDP(F)) was 0.999999999999995, and the combined mean exclusion chance in duo cases (CMEC(D)) was 0.999998 while in trio cases (CMEC(T)) was 0.999999986. The results suggest that the twelve ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545334 Thu, 22 Dec 2011 05:00:00 +0100 5545334 http://www.medworm.com/index.php?rid=5545333&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189545%26dopt%3DAbstract Authors: Zeng X, Yuan Z, Tong X, Li Q, Gao W, Qin M, Liu Z Abstract Oryzoideae (Poaceae) plants have economic and ecological value. However, the phylogenetic position of some plants is not clear, such as Hygroryza aristata (Retz.) Nees. and Porteresia coarctata (Roxb.) Tateoka (syn. Oryza coarctata). Comprehensive molecular phylogenetic studies have been carried out on many genera in the Poaceae. The different DNA sequences, including nuclear and chloroplast sequences, had been extensively employed to determine relationships at both higher and lower taxonomic levels in the Poaceae. Chloroplast DNA ndhF gene and atpB-rbcL spacer were used to construct phylogenetic trees and estimate the divergence time of Oryzoideae, Bambusoideae, Panicoideae, Pooideae and so on. Complete sequences ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5545333 Thu, 22 Dec 2011 05:00:00 +0100 5545333 http://www.medworm.com/index.php?rid=5527585&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187342%26dopt%3DAbstract In this study we isolated the zebrafish cDNA encoding xpd, and examined its spatial-temporal expression during early development as well as its tissue distribution in adult zebrafish. Only one gene was identified in zebrafish and its sequence analysis showed a molecular structure with 23 coding exons similar to other species. The amino acid sequences were also found to be largely conserved among all species analyzed, suggesting function maintenance throughout evolution. Gene expression analysis in different zebrafish tissues by qPCR showed xpd expression in all tissues examined with the highest expression in branchial arches. Analysis of xpd expression in zebrafish embryos showed maternal inheritance and presence of xpd transcripts in all developmental stages analyzed suggesting its implic... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527585 Wed, 21 Dec 2011 05:00:00 +0100 5527585 http://www.medworm.com/index.php?rid=5527581&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187343%26dopt%3DAbstract Authors: Ou-Yang Y, Li XL, Wang H, Fang M, Hu YJ Abstract The binding of palmatine to bovine serum albumin (BSA) was studied under physiological conditions (pH = 7.40) by molecular spectroscopic approach. It was proved that the fluorescence quenching of BSA by palmatine is a result of the formation of palmatine-BSA complex. Binding parameters were determined using the modified Stern-Volmer equation and Scatchard equation, to measure the specific binding between palmatine and BSA. The thermodynamic parameters calculated, ∆G°, ∆H° and ∆S° indicate that the electrostatic interactions play a major role in the palmatine-BSA association. Site marker competitive displacement experiments demonstrated that palmatine binds with specific affinity to site II (subdomain IIIA) of BSA.... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527581 Wed, 21 Dec 2011 05:00:00 +0100 5527581 http://www.medworm.com/index.php?rid=5527580&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187344%26dopt%3DAbstract Authors: Apostolidou E, Kambas K, Chrysanthopoulou A, Kourtzelis I, Speletas M, Ritis K, Mitroulis I Abstract Familial Mediterranean fever (FMF) is an autoinflammatory disease, characterized by MEFV gene mutations and self-limited recurrent episodes of fever and localized serositis. Complement system is a key regulator of the inflammatory process. The aim of this study was to investigate the genetic alterations and mRNA expression pattern of C5aR and C5L2 genes in neutrophils from attack-free FMF patients. No mutations were observed in the two receptors' genes, while the genetic alteration observed in the C5aR1 gene was identified as N279 K polymorphic variant. Furthermore, lower mRNA expression of C5L2 gene was observed in neutrophils from FMF patients compared to control subject... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527580 Wed, 21 Dec 2011 05:00:00 +0100 5527580 http://www.medworm.com/index.php?rid=5527579&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187345%26dopt%3DAbstract This study investigated the associations between type 2 diabetes and two single-nucleotide polymorphisms in the adiponectin (45T/G) and adiponectin receptor-2 gene (795G/A), and investigated whether these genetic variants affect the response to pioglitazone in Iranian patients with type 2 diabetes. We genotyped 128 non-diabetic participants and 101 patients with type 2 diabetes for 45T/G and 795G/A with polymerase chain reaction-restriction fragment length polymorphism assays. Patients were treated with pioglitazone for 12 weeks, after which we compared laboratory parameters in these two groups. Fasting blood sugar differed significantly in individuals with different 795G/A genotypes after pioglitazone treatment (P = 0.009). The mean decrease in insulin/glucose ratio after treatment als... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527579 Wed, 21 Dec 2011 05:00:00 +0100 5527579 http://www.medworm.com/index.php?rid=5527578&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187346%26dopt%3DAbstract Authors: Mao HT, Wang DH, Lan Z, Zhou H, Yang WX Abstract Prohibitin is essential for intracellular homeostasis and stabilization of mitochondrial respiratory chain complexes. To explore its functions during spermiogenesis of Octopus tankahkeei (O. tankahkeei), we have cloned and sequenced the cDNA of this mammalian PHB homologue (termed ot-PHB) from the testes of O. tankahkeei. The 1165 bp ot-phb cDNA contains a 100 bp 5' UTR, a 882 bp open reading frame and a 183 bp 3' UTR. The putative ot-PHB protein owns a transmembrane domain from 6 to 31 amino acid (aa) and a putative PHB domain from 26 to 178 aa. Protein alignment demonstrated that ot-PHB had 73.3, 73.6, 74.0, 75.1, and 45.4% identity with its homologues in Homo sapiens, Mus muculus, Danio rerio, Xenopus tropicalis and T... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527578 Wed, 21 Dec 2011 05:00:00 +0100 5527578 http://www.medworm.com/index.php?rid=5527577&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187347%26dopt%3DAbstract Authors: Li Z, Tatsuke T, Sakashita K, Zhu L, Xu J, Mon H, Lee JM, Kusakabe T Abstract Polycomb group (PcG) proteins are involved in chromatin modifications for maintaining gene repression that play important roles in the regulation of gene expression, tumorigenesis, chromosome X-inactivation, and genomic imprinting in Drosophila melanogaster, mammals, and even plants. To characterize the orthologs of PcG genes in the silkworm, Bombyx mori, 13 candidates were identified from the updated silkworm genome sequence by using the fruit fly PcG genes as queries. Comparison of the silkworm PcG proteins with those from other insect species revealed that the insect PcG proteins shared high sequence similarity. High-level expressions of all the silkworm PcG genes were maintained through day 2... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527577 Wed, 21 Dec 2011 05:00:00 +0100 5527577 http://www.medworm.com/index.php?rid=5527576&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187348%26dopt%3DAbstract Authors: Park M, Kim KS, Lee JH Abstract Lycorma delicatula (White) is native to China but is becoming an important insect pest in Korea. Polymorphic DNA markers like microsatellites are widely used for characterizing dispersal patterns and capacity of invasive insect pests which can contribute to designing effective management of the species. To facilitate such population genetic studies of L. delicatula in Korea, we isolated and characterized eight microsatellite loci for L. delicatula using a hybridization-biotin enrichment method. We further used these novel microsatellite loci to determine population genetic parameters for 33 L. delicatula specimens collected from Cheonan, South Korea where outbreaks of this species were first reported in Korea. The number of alleles per locus... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527576 Wed, 21 Dec 2011 05:00:00 +0100 5527576 http://www.medworm.com/index.php?rid=5527575&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187349%26dopt%3DAbstract In conclusion, BPA exposure can affect the formation of primordial follicle by inhibiting meiotic progression of oocytes. PMID: 22187349 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527575 Wed, 21 Dec 2011 05:00:00 +0100 5527575 http://www.medworm.com/index.php?rid=5527606&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183301%26dopt%3DAbstract Authors: Sliwinska A, Rogalska A, Szwed M, Kasznicki J, Jozwiak Z, Drzewoski J Abstract Experimental and clinical studies suggest that gliclazide may protect pancreatic β-cells from apoptosis induced by an oxidative stress. However, the precise mechanism(s) of this action are not fully understood and requires further clarification. Therefore, using human normal and cancer cells we examined whether the anti-apoptotic effects of this sulfonylurea is due to its free radical scavenger properties. Hydrogen peroxide (H(2)O(2)) as a model trigger of oxidative stress was used to induce cell death. Our experiments were performed on human normal cell line (human umbilical vein endothelial cell line, HUVEC-c) and human cancer cell lines (human mammary gland cell line, Hs578T; human pancreati... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527606 Tue, 20 Dec 2011 05:00:00 +0100 5527606 http://www.medworm.com/index.php?rid=5527605&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183302%26dopt%3DAbstract Authors: Pawlik P, Mostowska A, Lianeri M, Sajdak S, Kędzia H, Jagodzinski PP Abstract Data indicates that genetic factors alone do not account for ovarian tumorigenesis, suggesting that epigenetic status additionally affects this process. Therefore, we assessed the possible contribution of polymorphic variants of genes that may affect DNA methylation to the risk of ovarian cancer incidence in the Polish population. Using PCR-RFLP and HRM analyses, we studied the distribution of BHMT (rs3733890), MTHFD1 (rs2236225), MTHFR (rs1801133), MTR (rs1805087), MTRR (rs1801394) and TCN2 (rs1801198) genotypes and alleles in patients with ovarian cancer (n = 136) and controls (n = 160). Moreover, using DNA and methylation-specific PCR (MSP) we also determined the methylation of the Cadher... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527605 Tue, 20 Dec 2011 05:00:00 +0100 5527605 http://www.medworm.com/index.php?rid=5527604&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183303%26dopt%3DAbstract Authors: Lee YH, Lee HS, Choi SJ, Ji JD, Song GG Abstract The aim of this study was to determine whether the functional mannose-binding lectin (MBL2) exon 1 codon 54 polymorphism (rs1800450) confers susceptibility to systemic lupus erythematosus (SLE) in ethnically different populations. A meta-analysis was conducted on the MBL2 codon 54 polymorphism across 21 comparative studies. Meta-analysis showed an association between the MBL2 codon 54 B allele and SLE in all study subjects [odds ratio (OR) = 1.298, 95% confidence interval (CI) = 1.154-1.459, P = 1.4 × 10(-5)]. Analysis after stratification by ethnicity indicated that the MBL2 codon 54 B allele is significantly associated with SLE in Europeans, Asian, and Africans (OR = 1.246, 95% CI = 1.062-1.462, P = 0.007; O... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527604 Tue, 20 Dec 2011 05:00:00 +0100 5527604 http://www.medworm.com/index.php?rid=5527603&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183304%26dopt%3DAbstract Authors: Dang R, Zhu JQ, Tan FQ, Wang W, Zhou H, Yang WX Abstract KIF3B is known for maintaining and assembling cilia and flagellum. To date, the function of KIF3B and its relationship with KIF3A during spermiogenesis in the cephalopod Octopus tankahkeei remains unknown. In the present study, we characterized a gene encoding a homologue of rat KIF3B in the O. tankahkeei testis and examined its temporal and spatial expression pattern during spermiogenesis. The cDNA of KIF3B was obtained with degenerate and RACE PCR and the distribution pattern of ot-kif3b were observed with RT-PCR. The morphological development during spermiogenesis was illustrated by histological and transmission electron microscopy and mRNA expression of ot-kif3b was observed by in situ hybridization. The 2,365 nu... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527603 Tue, 20 Dec 2011 05:00:00 +0100 5527603 http://www.medworm.com/index.php?rid=5527596&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183305%26dopt%3DAbstract Authors: Li BH, Zhang LL, Yin YW, Pi Y, Yang QW, Gao CY, Fang CQ, Wang JZ, Li JC Abstract Epidemiological studies have evaluated the association between paraoxonase 2 (PON2) Ser311Cys polymorphism and ischemic stroke risk which developed inconsistent conclusions. The aim of this study was to perform a meta-analysis to investigate a more authentic association between PON2 Ser311Cys polymorphism and ischemic stroke. Systematic searches in PUBMED, EMBASE, CBM, and CNKI databases were performed. Data analyses were carried out by Review Manager 5.1.2 and Stata 11.0. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were used for additive model (Cys/Cys vs. Ser/Ser), dominant model (Ser/Cys+Cys/Cys vs. Ser/Ser), recessive model (Cys/Cys vs. Ser/Cys+Ser/Ser), and allelic mo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527596 Tue, 20 Dec 2011 05:00:00 +0100 5527596 http://www.medworm.com/index.php?rid=5527592&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183306%26dopt%3DAbstract This article briefly summarizes the current knowledge about MSC-based therapy for prevention or treatment of OA, discussing the direct intra-articular injection of MSCs for the treatment of OA in animal models and in clinical applications, as well as potential future strategies for OA treatment. PMID: 22183306 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527592 Tue, 20 Dec 2011 05:00:00 +0100 5527592 http://www.medworm.com/index.php?rid=5527586&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183307%26dopt%3DAbstract Authors: Wlodarczyk M, Nowicka G Abstract Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme-CYP1A1, phase II detoxifying enzymes-GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25-60 years old (44 ± 10 years). The level of DNA damage (% DNA in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1 gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+). PMID: 22... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527586 Tue, 20 Dec 2011 05:00:00 +0100 5527586 http://www.medworm.com/index.php?rid=5527610&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179747%26dopt%3DAbstract In this study, we explored the effects of SiO(2) nanoparticles (15, 30, and 100 nm) and their micro-sized counterpart on cultured human epidermal Keratinocyte (HaCaT) cells. Cell viability, cell morphology, reactive oxygen species (ROS), DNA damage (8-OHdG, γH2AX and comet assay) and apoptosis were assessed under control and SiO(2) nanoparticles exposed conditions. As observed in the Cell Counting Kit-8 (CCK-8) assay, exposure to 15, 30 or 100 nm SiO(2) nanoparticles at dosage levels between 0 and 100 μg/ml decreased cell viability in a concentration- and size dependent manner and the IC50 of 24 hour exposure was 19.4 ± 1.3, 27.7 ± 1.5 and 35.9 ± 1.6 μg/ml for 15, 30 and 100 nm SiO(2) nanoparticles, respectively. Morphological examination revealed cell shrinkage and cell ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527610 Sun, 18 Dec 2011 05:00:00 +0100 5527610 http://www.medworm.com/index.php?rid=5527609&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179748%26dopt%3DAbstract Authors: Kumla S, Doolgindachbaporn S, Sudmoon R, Sattayasai N Abstract Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to investigate the genetic structure of four subpopulations of Mystus nemurus in Thailand. The 7 RAPD and 7 ISSR primers were selected. Of 83 total RAPD fragments, 80 (96.39%) were polymorphic loci, and of 81 total ISSR fragments, 75 (92.59%) were polymorphic loci. Genetic variation and genetic differentiation obtained from RAPD fragments or ISSR fragments showed similar results. Percentage of polymorphic loci (%P), observed number of alleles, effective number of alleles, Nei's gene diversity (H) and Shannon's information index revealed moderate to high level of genetic variations within each M. nemurus subpopulati... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527609 Sun, 18 Dec 2011 05:00:00 +0100 5527609 http://www.medworm.com/index.php?rid=5527608&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179749%26dopt%3DAbstract In this study, two alternative 14-3-3 epsilon mRNAs, designated as 14-3-3EL and 14-3-3ES were identified from the shrimp L. vannamei. The 14-3-3EL isoform contains an insertion of 48 nucleotides by intron retention in the pre-mRNA of 14-3-3ε. While the 14-3-3ES occurred after being fully spliced. Using the yeast two hybrid method, the pattern of dimer formation by the two alternative 14-3-3ε isoforms revealed that the shrimp 14-3-3ε formed both homodimers and heterodimers. Both 14-3-3ε transcript variants were constitutively expressed in all shrimp tissues tested but the level of the 14-3-3ES isoform was always lower. However, after white spot syndrome virus (WSSV) infection, the expression level of the two transcript variants changed. At 48 h after infection, expression of 14-3-3EL m... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527608 Sun, 18 Dec 2011 05:00:00 +0100 5527608 http://www.medworm.com/index.php?rid=5527607&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179750%26dopt%3DAbstract Authors: Lee YH, Bae SC, Choi SJ, Ji JD, Song GG Abstract The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dominant model, and 4) an additive model. A total of 19 studies, 5,752 RA patients and 5,508 controls, encompassing 9 Caucasian, 8 Asian, 1 Mexican, and 1 Tunisian population were included in this meta-analysis. Ethnicity-specific meta-analysis was performed on Caucasian and Asian populations. Meta-analysis of the CTLA-4 +49 A/G polymorphism revealed an association between RA and the CTLA-4 +49 G allele in all 11,2... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527607 Sun, 18 Dec 2011 05:00:00 +0100 5527607 http://www.medworm.com/index.php?rid=5527612&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179692%26dopt%3DAbstract This study was aimed to search new genetic variants in the bovine FABP4 gene as molecular markers for meat quality and carcass traits. PCR-RFLP analysis revealed that three SNPs located at nucleotide positions g.2834C>G, g.3533T>A, and g.3691G>A were identified based on a GenBank accession number (NC_007312.4). Sequence analysis revealed that SNPs were located in intron 1 (g.2834C>G) and 2 (g.3533T>A), and an exon 3 (g.3691G>A), showing allele frequencies as 0.592, 0.579, and 0.789, respectively. Genetic variabilities of heterozygosity (He) and polymorphic information contents (PIC) were estimated for g.2834C>G (0.608 and 0.531), g.3533T>A (0.615 and 0.539), and g.3691G>A (0.498 and 0.401) loci, respectively. A SNP located in the exon 3 of FABP4 was characterized... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527612 Sat, 17 Dec 2011 05:00:00 +0100 5527612 http://www.medworm.com/index.php?rid=5527611&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179693%26dopt%3DAbstract Authors: Chen SJ, Luan J, Zhang HS, Ruan CP, Xu XY, Li QQ, Wang NH Abstract Despite the improvement of strategies against cancer therapy, the multidrug resistance (MDR)is the critical problem for successful cancer therapy. Recurrent cancers after initial treatment with chemotherapy are generally refractory to second treatments with these anticancer therapies. Therefore, it is necessary to elucidate the therapy-resistant mechanism for development of effective therapeutic modalities against tumors. Here we demonstrate a phase-specific chemotherapy resistance due to epidermal growth factor receptor (EGFR) in human breast cancer cells. Thymidine-induced G1-arrested cultures showed upregulated chemosensitivity, whereas S-phase arrested cells were more resistant to chemotherapeutic agent... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527611 Sat, 17 Dec 2011 05:00:00 +0100 5527611 http://www.medworm.com/index.php?rid=5527615&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173719%26dopt%3DAbstract Authors: Daraei A, Salehi R, Mohamadhashem F Abstract Colorectal cancer (CRC) is one of the leading cancers worldwide. Through genome wide association studies, several single nucleotide polymorphisms scattered in the genome emerged to be influential in the development of sporadic CRC in some populations. However, replicative studies failed to prove a particular SNP-CRC association in populations and ethnic groups. Cyclooxygenase-2 (PTGS2) is a crucial enzyme involved in the metabolism of prostaglandins. The aim of this replicative study is to investigate the possible association between PTGS2 -765G>C polymorphism and sporadic CRC risk in a subset of Iranian population. A total of 110 patients with sporadic CRC, and 120 controls were genotyped for PTGS2 -765G>C polymorphism ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527615 Fri, 16 Dec 2011 05:00:00 +0100 5527615 http://www.medworm.com/index.php?rid=5527614&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173720%26dopt%3DAbstract Authors: Parada R, Książkiewicz J, Kawka M, Jaszczak K Abstract The studies conducted aimed at evaluating the genetic diversity within and between varieties of conservative flocks of geese, using the polymorphism of 14 microsatellite sequences. The experimental material included conservative flocks of geese the following indigenous breeds and varieties kept in Poland: Kielecka (Ki), Kartuska (Ka), Lubelska (Lu), Suvalska (Su), Rypinska (Ry), Sub-Carpathian (SC), Hunched Beak (HB) and Pomeranian (Po). Among the 14 microsatellite sequences a total of 97 microsatellite alleles were identified. The number of alleles at one locus ranged from 3 to 19. In the overall pool of 97 alleles, 26 (26.8%) were specific for individual breeds and varieties of geese. The values of the expected het... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527614 Fri, 16 Dec 2011 05:00:00 +0100 5527614 http://www.medworm.com/index.php?rid=5527613&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173721%26dopt%3DAbstract We reported that the 140A/G LF (OR 4.78; 95% CI 3.07-7.24), the -33C/G OSF-2 OR 3.48; 95% CI 2.19-5.52) and the -33G/G OSF-2 (OR 16.45; 95% CI 6.71-40.30) genotypes were associated with an increased risk of chronic rhinosinusitis with nasal polyps among analyzed group of patients. Moreover, the group of patients without allergy or asthma indicated the association of the -33C/G (OR 3.72; 95% CI 2.24-6.19 and OR 15.11; 95% CI 5.91-38.6) and -33G/G (OR 3.73; 95% CI 2.24-6.19 and OR 14.07; 95% CI 5.47-36.16) genotypes of the OSF-2 as wells as 140A/G (OR 3.89; 95% CI 2.40-6.31 and OR 3.62; 95% CI 2.45-5.34) genotype of OSF-2 with an increased risk of chronic rhinosinusitis with nasal polyps. Finally, it was also found that the selected group of patients with allergy or asthma indicated a very s... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5527613 Fri, 16 Dec 2011 05:00:00 +0100 5527613 http://www.medworm.com/index.php?rid=5507731&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170597%26dopt%3DAbstract Authors: Gurgul A, Urszula C, Larska M, Polak MP, Strychalski J, Słota E Abstract Recent attempts to discover genetic factors affecting cattle resistance/susceptibility to bovine spongiform encephalopathy (BSE) have led to the identification of two insertion/deletion (indel) polymorphisms, located within the promoter and intron 1 of the prion protein gene PRNP, showing a significant association with the occurrence of classical form of the disease. Because the effect of the polymorphisms was studied only in few populations, in this study we investigated whether previously described association of PRNP indel polymorphisms with BSE susceptibility in cattle is also present in Polish cattle population. We found a significant relation between the investigated PRNP indel polymorphisms (2... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507731 Thu, 15 Dec 2011 05:00:00 +0100 5507731 http://www.medworm.com/index.php?rid=5507730&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170598%26dopt%3DAbstract In conclusion, BMP4-rs4444235 polymorphism is probably associated with CRC risk, and C allele is a possible risk factor in overall population. The same conclusion was drawn in Caucasians, but no significant results were obtained in other ethnic populations probably due to limited sample sizes. PMID: 22170598 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507730 Thu, 15 Dec 2011 05:00:00 +0100 5507730 http://www.medworm.com/index.php?rid=5507729&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170599%26dopt%3DAbstract Authors: Tian C, Liu T, Fang S, Du X, Jia C Abstract Oxidative damage promotes atherosclerosis. SOD2 is an important antioxidant enzyme. A case-control study and a meta-analysis were performed to assess the association of C47T polymorphism in SOD2 gene with premature, late-onset and overall coronary artery disease (CAD) risk. A hospital-based case-control study was conducted with 269 premature CAD cases, 278 late-onset CAD cases and 299 healthy controls. Polymerase chain reaction (PCR) and Pyrosequencing were used to detect the polymorphism. Multinomial logistic regression model was performed to estimate odds ratio (OR) with 95% confidence intervals (CIs) and adjust potential confounders. A meta-analysis was performed using eight outcomes including our result. Fixed or random effec... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507729 Thu, 15 Dec 2011 05:00:00 +0100 5507729 http://www.medworm.com/index.php?rid=5507728&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170600%26dopt%3DAbstract Authors: Guo DL, Zhang JY, Liu CH Abstract Start codon targeted (SCoT) polymorphic markers were used to assess genetic relationships among 64 grape varieties. Seventeen informative primers were selected from 36 SCoT primers based on their ability to produce clear and repeatable polymorphic and unambiguous bands among the varieties. A total of 131 bands were produced; 93.1% of them were polymorphic; the average polymorphism information content was 0.82. Cluster analysis of SCoT markers through the unweighted pair-group method of arithmetic averages analysis and principal coordinate analysis were largely consistent. The partition of clusters in the dendrogram and PCoA plot was similar and some degree of grouping by types of grape and taxonomic status of the varieties was revealed. Fo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507728 Thu, 15 Dec 2011 05:00:00 +0100 5507728 http://www.medworm.com/index.php?rid=5507727&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170601%26dopt%3DAbstract In this study, we explored whether polymorphisms (i.e. IL-4 C-590T, IL-4R Ile50Val, IL-4R Ser478Pro, IL-4R Gln551Arg, IL-13 C-1055T and IL-13 Arg130Gln) of IL-4, IL-4R and IL-13 genes predicted Chinese bladder cancer risk in 817 bladder cancer and 1,141 controls. Genotyping was performed by using the TaqMan method. We did not find any overall association between these single nucleotide polymorphisms (SNPs) and bladder cancer susceptibility in a Chinese population. However, in the classification and regression tree (CART) analysis, we found that carriers of IL-13 C-1055T variant genotype in smokers had a 2.57-fold increased bladder cancer risk with a 55% patient rate (OR = 2.57; 95% CI = 1.93-3.43), comparing with non-smokers. Similar result was also observed in combination of IL-13 C-1... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507727 Thu, 15 Dec 2011 05:00:00 +0100 5507727 http://www.medworm.com/index.php?rid=5507726&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170602%26dopt%3DAbstract Authors: Talarowska M, Gałecki P, Maes M, Gardner A, Chamielec M, Orzechowska A, Bobińska K, Kowalczyk E Abstract Oxidative stress has been implicated in the cognitive decline, especially in memory impairment. The purpose of this study was to determine the concentration of malondialdehyde (MDA) in patients with recurrent depressive disorders (rDD) and to define relationship between plasma levels of MDA and the cognitive performance. The study comprised 46 patients meeting criteria for rDD. Cognitive function assessment was based on: The Trail Making Test , The Stroop Test, Verbal Fluency Test and Auditory-Verbal Learning Test. The severity of depression symptoms was assessed using the Hamilton Depression Rating Scale (HDRS). Statistically significant differences were found in the... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507726 Thu, 15 Dec 2011 05:00:00 +0100 5507726 http://www.medworm.com/index.php?rid=5507725&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170603%26dopt%3DAbstract Authors: Biswas MK, Chai L, Mayer C, Xu Q, Guo W, Deng X Abstract The aim of this study was to develop a large set of microsatellite markers based on publicly available BAC-end sequences (BESs), and to evaluate their transferability, discriminating capacity of genotypes and mapping ability in Citrus. A set of 1,281 simple sequence repeat (SSR) markers were developed from the 46,339 Citrus clementina BAC-end sequences (BES), of them 20.67% contained SSR longer than 20 bp, corresponding to roughly one perfect SSR per 2.04 kb. The most abundant motifs were di-nucleotide (16.82%) repeats. Among all repeat motifs (TA/AT)n is the most abundant (8.38%), followed by (AG/CT)n (4.51%). Most of the BES-SSR are located in the non-coding region, but 1.3% of BES-SSRs were found to be associate... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507725 Thu, 15 Dec 2011 05:00:00 +0100 5507725 http://www.medworm.com/index.php?rid=5507740&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167326%26dopt%3DAbstract Authors: Kumar A, Sharma N, Panwar P, Gupta AK Abstract Fifty-two genotypes of Eleusine coracana collected from Uttarakhand hills were subjected to simple sequence repeat (SSR), random amplified polymorphic DNA (RAPD)-PCR and protein profiling analysis to investigate the variation in protein content. The main objective of the present study was to detect variability among E. coracana and also assess the discriminating ability of these three molecular methods. A total of 21 RAPD and 24 SSR primers were assayed for their specificity in detecting genetic variability in E. coracana, of which 20 RAPD and 21 SSR primers were highly reproducible and were found suitable for use in PCR analysis. Assessing genetic diversity among E. coracana genotypes by RAPD-PCR using 20 polymorphic primers ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507740 Wed, 14 Dec 2011 05:00:00 +0100 5507740 http://www.medworm.com/index.php?rid=5507739&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167327%26dopt%3DAbstract Authors: Seghatoleslam A, Nikseresht M, Shafiee SM, Monabati A, Namavari MM, Talei A, Safaei A, Owji AA Abstract The novel human gene, designated ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) maps to chromosome 1q21.3. The gene has an open reading frame corresponding to 422 amino acids and contains a RWD domain and an E2 ubiquitin conjugating enzyme domain. Here, we investigated the expression levels of both mRNA and protein of UBE2Q1 gene in cancerous versus normal parts of breast specimens from 26 patients. Real-time PCR data showed that the relative expression level of UBE2Q1 mRNA was significantly greater in cancers than in non-cancerous tissues of breast specimens (Mean ± SEM, 0.064 ± 0.015 for cancers and 0.026 ± 0.01 for noncancerous tissues, P < 0.... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507739 Wed, 14 Dec 2011 05:00:00 +0100 5507739 http://www.medworm.com/index.php?rid=5507738&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167328%26dopt%3DAbstract Authors: Baharum SN, Nurdalila AA Abstract The most economically important form of aquaculture is fish farming, which is an industry that accounts for an ever increasing share of world fishery production. Molecular markers can be used to enhance the productivity of the aquaculture and fish industries to meet the increasing demand. Molecular markers can be identified via a DNA test regardless of the developmental stage, age or environmental challenges experienced by the organism. The application of 16s and cytochrome b markers has enabled rapid progress in investigations of genetic variability and inbreeding, parentage assignments, species and strain identification and the construction of high resolution genetic linkage maps for aquaculture fisheries. In this review, the advantages ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507738 Wed, 14 Dec 2011 05:00:00 +0100 5507738 http://www.medworm.com/index.php?rid=5507737&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167329%26dopt%3DAbstract Authors: Mahmoudi T, Arkani M, Karimi K, Safaei A, Rostami F, Arbabi E, Pourhoseingholi MA, Mohebbi SR, Nikzamir A, Romani S, Almasi S, Abbaszadeh M, Vafaei M, Zali MR Abstract Vitamin D appears to have anti-tumor activities in the large bowel. Our aim was to investigate whether -4817 G>A (rs2238136) polymorphism located at 5'-untranslated region (5'-UTR) of the human vitamin D receptor (VDR) gene was associated with colorectal cancer (CRC) risk. We conducted a case-control study and VDR genotypes, determined by Bpu10I restriction endonuclease digestion of PCR-amplified DNA, were performed on 327 cases with CRC and 327 controls. The distribution of VDR -4817 G>A genotypes and alleles differed significantly between cases with CRC and controls even after adjustment for confound... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507737 Wed, 14 Dec 2011 05:00:00 +0100 5507737 http://www.medworm.com/index.php?rid=5507736&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167330%26dopt%3DAbstract Authors: Hong TT, Zhang RX, Wu XH, Hua D Abstract Published data on the association of vascular endothelial growth factor (VEGF) -1154G>A polymorphism with cancer risk is inconclusive. To derive a more precise estimation of association between VEGF -1154G>A polymorphism and the risk of cancer, we performed a meta-analysis of 7,071 cancer cases and 7,693 controls from 16 published case-control studies. Our meta-analysis didn't reveal an association between VEGF -1154G>A polymorphism and overall cancer risk (GG vs. AA: OR: 1.08, 95% CI: 0.96-1.20; GA vs. AA: OR: 1.04, 95% CI: 0.93-1.17; recessive model: GG+GA vs. AA: OR: 1.06, 95% CI: 0.95-1.18; dominant model: GG vs. GA+AA, OR: 1.11, 95% CI: 1.00-1.24). Nevertheless, for non-Caucasians, GG homozygote may have higher cancer ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507736 Wed, 14 Dec 2011 05:00:00 +0100 5507736 http://www.medworm.com/index.php?rid=5507735&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167331%26dopt%3DAbstract In conclusion, this compound could be proposed as an antihyperglycemic and antihyperlipidemic agent in diabetes and potential therapeutic in obesity. PMID: 22167331 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507735 Wed, 14 Dec 2011 05:00:00 +0100 5507735 http://www.medworm.com/index.php?rid=5507734&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167332%26dopt%3DAbstract Authors: Zheng ST, Zhang CS, Qin X, Gen YH, Liu T, Sheyhidin I, Lu XM Abstract The p38 mitogen-activated protein kinase (MAPK) is a member of the MAPK family, which is initially found to be activated by stress stimuli, proinflammatory cytokines, and growth factors. However, its role in the pathogenesis of esophageal squamous cell carcinoma (ESCC) is largely unkown, so we investigate the role of phosphorylated p38 (p-p38) MAPK in ESCC. First of all, in vitro cell line ECa109, SB203580 as selective inhibitor of p38, can suppress the growth of esophageal cancer cell in a dose- and time-dependent way, suggesting that ECa109 cell growth and proliferation was closely associated with p-p38. Using western-blot analysis of fresh 16 paired surgically resected ESCC and matched non-tumor adjac... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507734 Wed, 14 Dec 2011 05:00:00 +0100 5507734 http://www.medworm.com/index.php?rid=5507733&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167333%26dopt%3DAbstract This study supports the hypothesis that the lymphatic microvessel count or LVD, which reflects levels of lymphangiogenesis, is a poor prognostic factor for patient survival in surgically treated NSCLC. However, the present findings may overestimate the prognostic capacity of LVD because of publication and report bias. In addition, the standardization of lymphangiogenesis assessment by the lymphatic microvessel count is necessary. PMID: 22167333 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507733 Wed, 14 Dec 2011 05:00:00 +0100 5507733 http://www.medworm.com/index.php?rid=5507732&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167334%26dopt%3DAbstract In this study, 40 wild type KRAS and BRAF colorectal tumors were analyzed to elucidate whether PML-RARa bcr1 fusion gene may play a role in colorectal carcinogenesis. Specifically we want to determine if this fusion could be responsible for the inability to respond to anti-EGFR monoclonal antibodies. Here, for the first time it is reported, that PML-RARa bcr1 fusion is not responsible for colorectal tumor development and also, this translocation is not predicting the lack of efficacy of anti-EGFR therapies in wild type KRAS and BRAF colorectal cancer patients. These results also suggest that PML-RARa is unlikely to be a promising target for adjuvant therapy in colorectal cancer patients. PMID: 22167334 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507732 Wed, 14 Dec 2011 05:00:00 +0100 5507732 http://www.medworm.com/index.php?rid=5507766&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160467%26dopt%3DAbstract Authors: Bang K, Park S, Yoo JY, Cho S Abstract To isolate antimicrobial-related genes from the beet armyworm, Spodoptera exigua, we performed GeneFishing, a polymerase chain reaction (PCR)-based differential display technique. An attacin-like complementary DNA (cDNA) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. The full-length attacin cDNA from S. exigua cDNA (Seattacin) was cloned using rapid amplification of cDNA ends PCR. The attacin-like cDNA transcript was 765 nucleotides in length, and the predicted polypeptide was 254 amino acids in length with a calculated molecular mass of 27.6 kDa and an isoelectric point of 6.44. The protein sequence of the attacin-like cDNA showed high identity to that of Trichoplus... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507766 Tue, 13 Dec 2011 05:00:00 +0100 5507766 http://www.medworm.com/index.php?rid=5507765&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160468%26dopt%3DAbstract Authors: Waluś-Miarka M, Sanak M, Idzior-Waluś B, Miarka P, Witek P, Małecki MT, Czarnecka D Abstract The purpose of this investigation was to characterize a new mutation in the LDL-receptor (LDLR) gene in three families with clinically diagnosed familial hypercholesterolemia (FH) from the South-Eastern part of Poland. Mutational screening with exon by exon sequencing analysis was performed in all probands. The novel mutation c986G>T (Cys308Phe) in the exon 7 of LDLR gene was found in three apparently unrelated probands with FH. Analysis of the receptor activity of peripheral blood lymphocytes by binding and uptake of DiL-LDL showed a significant reduction (by 24% versus healthy control) of the fluorescent label in the lymphocytes of patients heterozygous for this mutation. C... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507765 Tue, 13 Dec 2011 05:00:00 +0100 5507765 http://www.medworm.com/index.php?rid=5507764&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160469%26dopt%3DAbstract Authors: Chen X, Huang Z, Chen D, Jia G, Mao X, Wu X Abstract Insulin resistance is a clinical condition that is characterized by reducing glucose uptake in response to insulin. A major factor in the development of insulin resistance syndrome is obesity. NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects. NYGGF4 induced the secretion of FFAs and TNF-α and caused mitochondrial dysfunction, which may cause insulin resistance. This review will summarize the effect of NYGGF4 on the adipogenesis, glucose uptake and mitochondrial dysfunction in vitro, and the possible mechanism and signal pathway of NYGGF4 for insulin resistance. PMID: 22160469 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507764 Tue, 13 Dec 2011 05:00:00 +0100 5507764 http://www.medworm.com/index.php?rid=5507763&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160470%26dopt%3DAbstract Authors: Zhao Y, Zhou Y, Xiong N, Lin Z Abstract The human dopamine transporter gene (hDAT) encodes the dopamine transporter in dopamine (DA) neurons to regulate DA transmission. hDAT expression varies significantly from neuron to neuron, and from individual to individual so that dysregulation of hDAT is related to many neuropsychiatric disorders. It is critical to identify hDAT-specific cis-acting elements that regulate the hDAT expression. Previous studies showed that hDAT Intron 1 displayed inhibitory activity for reporter gene expression. Here we report that the hDAT Intron 1 contains a 121-bp fragment that down-regulated both SV40 and hDAT promoter activities by 80% in vitro. Subfragments of 121-bp still down-regulated the SV40 promoter but not the hDAT promoter, as supported ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507763 Tue, 13 Dec 2011 05:00:00 +0100 5507763 http://www.medworm.com/index.php?rid=5507762&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160471%26dopt%3DAbstract Authors: Wu ZQ, Sun L, Sun YH, Ren C, Jiang YH, Lv XL Abstract One previous meta-analysis found no evidence that interleukin 1 beta (IL-1β) -511 gene polymorphism was associated with febrile seizures (FS) by pooling a limited number of studies. However, it is necessary for the meta-analysis to reevaluate the relationship with more recent findings. Electronic databases were systematically searched for studies published before June 2011. Pooled odds ratios (OR) and 95% confidence interval (CI) were estimated by means of a genetic model free approach. Subgroup and sensitivity analyses were also performed. All statistical analyses were conducted using Stata 9.0. A total of eight studies, 728 FS cases and 1,223 controls, met the selection criteria. The results show a significant associ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507762 Tue, 13 Dec 2011 05:00:00 +0100 5507762 http://www.medworm.com/index.php?rid=5507761&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160472%26dopt%3DAbstract We report here on the isolation of the 5'-flanking region (1,049 bp) of the hydroxymethylbutenyl 4-diphosphate synthase (HDS) gene from the MEP pathway. To investigate promoter transcriptional activities, the HDS promoter was fused to GUS reporter gene. Agrobacterium-mediated transformation of young tobacco leaves revealed that the cloned HDS promoter displays a tissue-specific GUS staining restricted to the vascular region of the leaves and limited to a part of the vein that encompasses the phloem in agreement with the previous localization of HDS transcripts in C. roseus aerial organs. Further functional characterizations in stably or transiently transformed C. roseus cells allowed us to identify the region that can be consider as the minimal promoter and to demonstrate the induction of... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507761 Tue, 13 Dec 2011 05:00:00 +0100 5507761 http://www.medworm.com/index.php?rid=5507768&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160430%26dopt%3DAbstract Authors: Kamal AH, Cho K, Komatsu S, Uozumi N, Choi JS, Woo SH Abstract We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This enabled us to detect and identify 767 unique proteins. Our findings represent the most comprehensive exploration of a proteome to date. Based on annotation information from the UniProtKB/Swiss-Prot database and our analyses via WoLF PSORT and PSORT, these proteins are localized in the chloroplast (607 proteins), chloroplast stroma (145), thylakoid membrane (342), lumens (163), and integral membranes... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507768 Sun, 11 Dec 2011 05:00:00 +0100 5507768 http://www.medworm.com/index.php?rid=5507767&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160431%26dopt%3DAbstract Authors: Sun J, Liu HP, Deng JE, Zhou M Abstract The clustering propensity of microRNA genes is a common biological phenomenon in various animal and plant species. To gain novel insight into genomic organization and potential functional heterogeneities of miRNA clusters in vertebrates from a genome scale, we used large scale data and presented a comprehensive analysis to examine various features of genomic organization of miRNA clusters across seven vertebrates by a combination of comparative genomics and bioinformatics approaches. The results of pair-wise distance analysis of same-strand consecutive miRNAs suggested that the fractions of the miRNA gene pairs are higher at relatively short pair-wise distances than those of protein-coding genes and other non-coding RNA genes. Especi... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507767 Sun, 11 Dec 2011 05:00:00 +0100 5507767 http://www.medworm.com/index.php?rid=5507745&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161245%26dopt%3DAbstract In this study, genetic variability at codon 42 within prion protein (PRNP) gene and its associations with production traits were investigated in 2002 goats from four Chinese domestic breeds. The frequencies of allele "A" ranged from 0.353 to 0.562 in analyzed goat breeds with Hardy-Weinberg equilibrium (P > 0.05) except Xinong Sannen (XNSN) dairy breed. The establishment of relationships between different genotypes and growth traits was performed in Inner Mongolia white Cashmere (IMWC) breed and revealed an association of the polymorphism with body weight at 7-year-old goats (P = 0.033). The individuals with genotype GG showed heavier body weight than those with genotype AA. Moreover, association analysis detected two significant associations between different genotypes and cashmere... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507745 Sat, 10 Dec 2011 05:00:00 +0100 5507745 http://www.medworm.com/index.php?rid=5507744&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161246%26dopt%3DAbstract In this study, we focused on the inhibition of Notch2 function in B-cell chronic lymphocytic leukemia (B-CLL) by AS-ODN (phosphorothioate oligomers) targeted to the initiation codon region of the Notch2 mRNA. We investigated the in vitro ability of four such oligomers to reduce the expression of Notch2 gene in peripheral blood mononuclear cells from B-CLL patients. Our findings implicate that AS-ODNs specifically designed for the region of 314-333 neucleotides (AS1) of Notch2 inhibits its gene expression better than other AS-ODNs designed for other regions and respond in a dose dependent manner. The results of cell proliferation assay for the evaluation of AS1 in gene silencing, infer that the number of cells were reduced to 80% (P < 0.001). Our results implicate that using the AS-ODN... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507744 Sat, 10 Dec 2011 05:00:00 +0100 5507744 http://www.medworm.com/index.php?rid=5507743&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161247%26dopt%3DAbstract Authors: Zhang L, Wang JN, Tang JM, Kong X, Yang JY, Zheng F, Guo LY, Huang YZ, Zhang L, Tian L, Cao SF, Tuo CH, Guo HL, Chen SY Abstract Vascular endothelial growth factor (VEGF) plays a crucial role in tumor angiogenesis. VEGF induces new vessel formation and tumor growth by inducing mitogenesis and chemotaxis of normal endothelial cells and increasing vascular permeability. However, little is known about VEGF function in the proliferation, survival or migration of hepatocellular carcinoma cells (HCC). In the present study, we have found that VEGF receptors are expressed in HCC line BEL7402 and human HCC specimens. Importantly, VEGF receptor expression correlates with the development of the carcinoma. By using a comprehensive approaches including TUNEL assay, transwell and wound ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507743 Sat, 10 Dec 2011 05:00:00 +0100 5507743 http://www.medworm.com/index.php?rid=5507742&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161248%26dopt%3DAbstract Authors: He XF, Wei W, Su J, Yang ZX, Liu Y, Zhang Y, Ding DP, Wang W Abstract The previous published data on the association between X-ray repair cross-complementing group 3 (XRCC3) T241M, A4541G, and A17893G polymorphisms and breast cancer risk remained controversial. Hence, we performed a meta-analysis to investigate the association between breast cancer and XRCC3 T241M (21,910 cases and 23,961 controls), A4541G (9,633 cases and 10,994 controls), and A17893G polymorphisms (10,761 cases and 12,235 controls) in different inheritance models. When all the eligible studies were pooled into the meta-analysis of XRCC3 T241M polymorphism, significantly increased risk of breast cancer was observed in recessive model (odds' ratio [OR] = 1.10, 95% confidence interval [CI] = 1.04-1.16) ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507742 Sat, 10 Dec 2011 05:00:00 +0100 5507742 http://www.medworm.com/index.php?rid=5507741&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161249%26dopt%3DAbstract Determination of combined sibship indices "gray zone" using 15 STR loci for central Bosnian human population. Mol Biol Rep. 2011 Dec 10; Authors: Musanovic J, Filipovska-Musanovic M, Kovacevic L, Buljugic D, Dzehverovic M, Avdic J, Marjanovic D Abstract In our previous population studies of Bosnia and Herzegovina human population, we have used autosomal STR, Y-STR, and X-STR loci, as well as Y-chromosome NRY biallelic markers. All obtained results were included in Bosnian referent database. In order of future development of applied population molecular genetics researches of Bosnia and Herzegovina human population, we have examined the effectiveness of 15 STR loci system in determination of sibship by using 15 STR loci and calculating different cut-off points of combined si... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507741 Sat, 10 Dec 2011 05:00:00 +0100 5507741 http://www.medworm.com/index.php?rid=5507753&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160569%26dopt%3DAbstract Authors: Zhang HM, Fei ZH, Tang BP, Chen J, Tao WH, Wang YQ Abstract The features of brucine (BC) binding to two blood proteins, bovine hemoglobin (BHb), and bovine serum albumin (BSA), were investigated via fluorescence, circular dichroism and UV/Vis absorption spectroscopy. The results revealed that BC caused the fluorescence quenching of blood proteins by the formation of BC-protein complex. The corresponding thermodynamic parameters were measured at different temperatures. The process of binding BC molecule on protein was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Hydrophobic and electrostatic interactions play a major role in stabilizing the complex. The molecular docking has been employed to explore the binding si... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=5507753 Fri, 09 Dec 2011 05:00:00 +0100 5507753