MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Molecular Biology Reports' source. Wed, 17 Mar 2010 16:34:12 +0100 http://www.medworm.com/index.php?rid=3371141&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20232157%26dopt%3DAbstract Authors: Wang Y, Deng D, Bian Y, Lv Y, Xie Q The phytohormone auxin is important in various aspects of organism growth and development. Aux/IAA genes encoding short-lived nuclear proteins are responsive primarily to auxin induction. Despite their physiological importance, systematic analysis of Aux/IAA genes in maize have not yet been reported. In this paper, we presented the isolation and characterization of maize Aux/IAA genes in whole-genome scale. A total of 31 maize Aux/IAA genes (ZmIAA1 to ZmIAA31) were identified. ZmIAA genes are distributed in all the maize chromosomes except chromosome 2. Aux/IAA genes expand in the maize genome partly due to tandem and segmental duplication events. Multiple alignment and motif display results revealed major maize Aux/IAA proteins share all th... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3371141 Tue, 16 Mar 2010 00:00:00 +0100 3371141 http://www.medworm.com/index.php?rid=3371140&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20232158%26dopt%3DAbstract The objectives of the present study were focused on detecting deletion mutation in bovine AMPD1 gene, and analyzing its effect on body measurement and carcass traits in Qinchuan cattle by using DNA sequencing and agarose electrophoresis methods. The 198-bp PCR products of AMPD1 gene exhibited three genotypes and two alleles were revealed: A and B. The frequencies of genotype AA/AB/BB in Qinchuan populations was 0.7163, 0.2233 and 0.0605. The chi(2)-test analysis demonstrated that the breed was not in agreement with Hardy-Weinberg equilibrium (P < 0.05). The association of the 18-bp deletion mutation of AMPD1 gene with body measurement and carcass traits of Qinchuan cattle were analyzed. The cattle with AA genotype had slaughter weight and carcass weight than those with genotype AB (P &l... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3371140 Tue, 16 Mar 2010 00:00:00 +0100 3371140 http://www.medworm.com/index.php?rid=3371139&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20232159%26dopt%3DAbstract In this study, we cloned and sequenced partial cDNA, intron 1 and 5'-flanking sequences of porcine APC10. The partial cDNA is 595 bp long and has an open reading frame of 558 bp which encodes 185 putative animo acids. Real-time PCR analysis revealed that the porcine APC10 mRNA expression shows a wide distribution and expression levels varies within a small different range in detected tissues. The deduced protein has a high identity with other eight species and comprises a conserved DOC domain. The phylogenetic tree indicated that porcine APC10 has the closest genetic relationship with human, monkey and dog. Promoter activity was demonstrated by transient transfection of 5'-deletion promoter/luciferase constructs into PK15 cells, and indicated that the proximal region from -2,052 to -1,764 ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3371139 Tue, 16 Mar 2010 00:00:00 +0100 3371139 http://www.medworm.com/index.php?rid=3371143&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20229016%26dopt%3DAbstract In this study, the expression of Ang2 in Pichia pastoris expression system was described for the first time. The cDNA encoding Ang2 was cloned from the rat vascular tissue by RT-PCR, and inserted in the eukaryotic expression vector pPIZalphaA, and then transformed into P. pastoris KM71H cells. The expression of recombinant rat Ang2 (rrAng2) was induced by methanol and accounted for about 75% of the total secreted proteins. The recombinant protein was subsequently purified by HisTrap FF crude with a purity of 90%. Functional analysis of the purified rrAng2 demonstrated a specific activity in promoting the survival of ECV304 cells and binding to the Tie2 receptor. Preparation of bioactive rrAng2 not only lays the basis for further functional study but also provides a new strategy for soluble... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3371143 Sat, 13 Mar 2010 00:00:00 +0100 3371143 http://www.medworm.com/index.php?rid=3371142&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20229017%26dopt%3DAbstract In conclusion, our data indicate that STAT3 plays a critical role in IL-6-induced TFF3 expression in human BECs and the IL-6/STAT3/TFF3 signaling is involved in human BEC migration and wound healing. PMID: 20229017 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3371142 Sat, 13 Mar 2010 00:00:00 +0100 3371142 http://www.medworm.com/index.php?rid=3362635&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20224909%26dopt%3DAbstract In this study we show that TNFalpha, but not LPS, was significantly able to stimulate the production of NO in HSV-tk transduced 9L glioblastoma cell lines, mediated by the up-regulation of iNOS transcript and iNOS protein. The TNFalpha-induced up-regulation of iNOS expression was mediated by MAPK and NF-kappaB signaling pathways as revealed by using selective pharmaceutical inhibitors. A culture liquid extract of the edible and medicinal mushroom Marasmius oreades that was previously shown to inhibit iNOS expression in MCF-7 was utilized to prepare fractions and evaluate their ability to affect TNFalpha-induced iNOS expression in HSV tk transduced 9L cell lines. While most of the tested fractions were shown to inhibit TNFalpha-induced iNOS expression, they targeted different signaling path... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3362635 Fri, 12 Mar 2010 00:00:00 +0100 3362635 http://www.medworm.com/index.php?rid=3362638&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20221700%26dopt%3DAbstract In this study, the homology for mouse Ipr1 in canines was found to have an extra domain structure, h.1.5.1. And 30 potential candidate proteins were predicted to bind canine Ipr1, which were characterized of the interacting structure with the h.1.5.1. Among them, MYBBP1A was verified to bind with both Ipr1 and eGFP-Ipr1 in mouse macrophage J774A.1 clone 21 cells using co-immunoprecipitation method. And with the constructed high-confidence Ipr1-involved network, we suggested that Ipr1 might be involved in apoptosis pathway via MYBBP1A. PMID: 20221700 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3362638 Thu, 11 Mar 2010 00:00:00 +0100 3362638 http://www.medworm.com/index.php?rid=3362637&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20221701%26dopt%3DAbstract Authors: Sudheer PD, Rahman H, Mastan SG, Reddy MP Jatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3362637 Thu, 11 Mar 2010 00:00:00 +0100 3362637 http://www.medworm.com/index.php?rid=3362636&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20221702%26dopt%3DAbstract Authors: Lakra WS, Goswami M, Singh A, Mishra P, Gayathri N, Nagpure NS Genetic structure of four populations of Metapenaeus affinis from Maharashtra, Orissa, Kerala and Tamil Nadu in India was studied using RAPD markers. Five selective primers provided distinct and consistent RAPD profiles in all the four populations. The bands in the range 225-1,900 bp were scored for consistent results. The RAPD profiles generated by all the five primers revealed varying degrees of polymorphism, ranging from 25.00% (primer E-03) to 65.00% (primer E-06). Nei's (Nei M, Natl Acad Sci Proc USA 70:3321-3323, 1973) genetic diversity (h) among the four populations varied from 0.2565 +/- 0.2146 (Orissa population) to 0.3576 +/- 0.1897 (Maharashtra population). PMID: 20221702 [PubMed - as supplied by pub... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3362636 Thu, 11 Mar 2010 00:00:00 +0100 3362636 http://www.medworm.com/index.php?rid=3354781&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217239%26dopt%3DAbstract Authors: Ajmal M, Ahmed W, Sadeque A, Ali SH, Bokhari SH, Ahmed N, Qamar R Familial Hypercholesterolemia (FH) results in elevated levels of blood lipids including total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) with normal triglycerides (TG). This disease is one of the major contributors towards an early onset of coronary heart disease (CHD). The aim of the present study was to identify the genes responsible for causing FH in Pakistani population, for this purpose a large consanguineous FH family was selected for genetic analysis. Serum lipid levels, including TC, TG, LDL-C and high density lipoprotein cholesterol (HDL-C), were determined in patients and healthy controls. In order to find the causative mutation in this family, direct sequencing of the low density... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354781 Wed, 10 Mar 2010 00:00:00 +0100 3354781 http://www.medworm.com/index.php?rid=3354779&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217241%26dopt%3DAbstract In this study, we report the discovery and development of gene-targeted single nucleotide polymorphisms (SNPs) for genomic mapping in the Pacific abalone Haliotis discus hannai Ino. Single EST or EST-contigs from 66 genes that had positive BLASTx matches (E-value </= 1e-8) were used for polymerase chain reaction (PCR) amplification. PCR products from the two parents of one mapping family were directly sequenced, and 83 SNP loci were found from 17 genes. Allele-specific PCR (AS-PCR) was developed and optimized for genotyping of 11 SNP loci in 120 progeny of the mapping family. Nine of the loci conformed to the expected Mendelian ratio of 1:1 based on the chi(2) test (P > 0.05) and could potentially be used for linkage map construction. Our data also indicate that the sequencing of two... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354779 Wed, 10 Mar 2010 00:00:00 +0100 3354779 http://www.medworm.com/index.php?rid=3354778&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217242%26dopt%3DAbstract Authors: Fang NX, Yao YT, Shi CX, Li LH Volatile anesthetic ischemic postconditioning reduces infarct size following ischemia/reperfusion. Whether phosphorylation of protein kinase B (PKB/Akt) and glycogen synthase kinase 3 beta (GSK3beta) is causal for cardioprotection by postconditioning is controversial. We therefore investigated the impact of PKB/Akt and GSK3beta in isolated perfused rat hearts subjected to 40 min of ischemia followed by 1 h of reperfusion. 2.0% sevoflurane (1.0 minimum alveolar concentration) was administered at the onset of reperfusion in 15 min as postconditioning. Western blot analysis was used to determine phosphorylation of PKB/Akt and its downstream target GSK3beta after 1 h of reperfusion. Mitochondrial and cytosolic content of cytochrome C checked by weste... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354778 Wed, 10 Mar 2010 00:00:00 +0100 3354778 http://www.medworm.com/index.php?rid=3354777&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217243%26dopt%3DAbstract Authors: Ricachenevsky FK, Sperotto RA, Menguer PK, Fett JP Fe participates in several important reactions in plant metabolism. However, Fe homeostasis in plants is not completely understood, and molecular studies on Fe-excess stress are scarce. Rice (Oryza sativa L. ssp. indica) is largely cultivated in submerged conditions, where the extremely reductive environment can lead to severe Fe overload. In this work, we used representational difference analysis (RDA) to isolate sequences up-regulated in rice shoots after exposure to Fe-excess. We isolated 24 sequences which have putative functions in distinct cellular processes, such as transcription regulation (OsWRKY80), stress response (OsGAP1, DEAD-BOX RNA helicase), proteolysis (oryzain-alpha, rhomboid protein), photosynthesis (chlorop... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354777 Wed, 10 Mar 2010 00:00:00 +0100 3354777 http://www.medworm.com/index.php?rid=3354791&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213504%26dopt%3DAbstract Authors: Ghaffar A, Khan SA, Mukhtar Z, Rajoka MI, Latif F We studied heterologous expression of xylanase 11A gene of Chaetomium thermophilum in Pichia pastoris and characterized the thermostable nature of the purified gene product. For this purpose, the xylanase 11A gene of C. thermophilum was cloned in P. pastoris GS115 under the control of AOX1 promoter. The maximum extracellular activity of recombinant xylanase (xyn698: gene with intron) was 15.6 U ml(-1) while that of recombinant without intron (xyn669) was 1.26 U ml(-1) after 96 h growth. The gene product was purified apparently to homogeneity level. The optimum temperature of pure recombinant xylanase activity was 70 degrees C and the enzyme retained its 40.57% activity after incubation at 80 degrees C for 10 min. It exhibited q... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354791 Tue, 09 Mar 2010 00:00:00 +0100 3354791 http://www.medworm.com/index.php?rid=3354785&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213510%26dopt%3DAbstract In this study, we detected the polymorphism of Src gene by polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) and DNA sequencing methods in 985 individuals from three Chinese cattle breeds. Three novel single nucleotide polymorphisms (SNPs) (g.14062C>T ss161151834, g.17302G>A ss161151835, g.18107T>C ss161151836) were detected. Least squares analysis showed that cows with g.14062C>T-CC genotypes and g.18107T>C-TT genotypes had the highest protein rate, while the cows with g.17302G>A-GG genotype had higher 305 d milk yield (p < 0.05), fat yield (p < 0.01) and protein yield (p < 0.01) than the ones with genotypes g.17302G>A-GA. These results revealed the statistical significant effects of three SNPs of the Src gene on the milk pro... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354785 Tue, 09 Mar 2010 00:00:00 +0100 3354785 http://www.medworm.com/index.php?rid=3354780&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217240%26dopt%3DAbstract Authors: Du D, Shi YH, Le GW High glycemic index diet can induce multiple diseases. Many research indicated that oxidative stress played important role in many pathological conditions. However, the impact of gene expression and dietary habit on oxidation process are still less clear. We used high-glucose diet to feed C57BL/6J mice for 4 weeks, measured the redox status, physiological and biochemical changes related to diabetes and consequence of metabolic syndrome (nonalcoholic fatty liver, cardiovascular disease), and detected the expressions of 14,446 genes in liver of C57BL/6J mice with DNA microarray. The results showed high-glucose diet induced elevated fatty acid accumulation in liver, insulin resistance index and higher weight in C57BL/6J mice, which indicated high-glucose diet ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354780 Tue, 09 Mar 2010 00:00:00 +0100 3354780 http://www.medworm.com/index.php?rid=3354789&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213506%26dopt%3DAbstract Authors: Feng X, Liu DF, Wang NX, Zhu CD, Jiang GF The nearly complete mitochondrial genome of the butterfly Papilio xuthus (Lepidoptera: Papilionidae) was sequenced for its nucleotide sequence of 13,964 bp. The genome has a typical gene order identical to other lepidopteran species. All tRNAs showed same stable canonical clover-leaf structure as those of other insects, except for tRNA(Ser) (AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Anomalous initiation codons have been observed for the cox1 gene, where the ATTACG hexa-nucleotide was believed to be involved in the initiation signaling. Twelve mitochondrial protein-coding gene sequence data were used to infer the phylogenetic relationships among the insect orders. Even though the number of... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354789 Mon, 08 Mar 2010 00:00:00 +0100 3354789 http://www.medworm.com/index.php?rid=3354787&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213508%26dopt%3DAbstract Authors: Hu YJ, Ou-Yang Y, Dai CM, Liu Y, Xiao XH Fluorescence spectroscopy in combination with UV-vis absorption spectroscopy was employed to investigate the binding of an important traditional medicinal herb berberine to bovine serum albumin (BSA) under the physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by berberine is a result of the formation of berberine-BSA complex. Fluorescence quenching constants were determined using the Stern-Volmer equation and Scatchard equation to provide a measure of the binding affinity between berberine and BSA. The results of thermodynamic parameters DeltaG, DeltaH, DeltaS at different temperatures indicate that the electrostatic interactions play a major role for berberine-BSA association. S... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354787 Mon, 08 Mar 2010 00:00:00 +0100 3354787 http://www.medworm.com/index.php?rid=3354790&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213505%26dopt%3DAbstract Authors: Liu G, Fang Y, Zhang H, Li Y, Li X, Yu J, Wang X MicroRNAs (miRNAs) belong to a class of small non-coding RNAs that play important roles in complex biological processes through degradation of target mRNAs or repression of their translation. We exploited cross-species comparison to predict miRNAs and identified 266 genes encoding 274 mature miRNAs in Oryctolagus cuniculus. Comparative analyses among mammalian genomes demonstrated that most of the identified miRNAs and their clusters are ancient in origin and conserved among mammals but a few clades as well as some species-specific miRNAs exhibit an ongoing evolutionary process where gain and loss of individual miRNAs have occurred through tandem duplications and random mutations. Our microarray- and RT-PCR-based analyses and ta... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354790 Sun, 07 Mar 2010 00:00:00 +0100 3354790 http://www.medworm.com/index.php?rid=3354788&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213507%26dopt%3DAbstract Authors: Wan L, Xia X, Hong D, Yang G High throughput screening of stage-specific differentially expressed genes in a Brassica napus two-line Rs1046A/B subtractive library was used to identify the BnQRT3 gene associated with cell wall metabolism. Phylogenetic analysis indicates the protein product of BnQRT3 is polygalacturonase. According to cytological comparisons of Rs1046 sterile and fertile anthers, RT-PCR studies and in situ hybridizations, BnQRT3 is expressed most strongly in floral organs and may play an essential role in pollen maturation. Analysis of the histological staining pattern of BnQRT3 promoter-GUS constructs in transgenic Arabidopsis and Brassica napus revealed that proximal part of 5'-flanking region directed expression in the vascular tissue of filaments, veins in s... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354788 Sun, 07 Mar 2010 00:00:00 +0100 3354788 http://www.medworm.com/index.php?rid=3354786&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213509%26dopt%3DAbstract Authors: Guo F, Liu Y, Li Y, Li G ADP-ribosylation factor-like 6 interacting protein 1 (ARL6IP1) is an apoptotic regulator. To investigate the role of ARL6IP1 in human cervical cancer progression, we designed and used short hairpin RNA (shRNA) to inhibit ARL6IP1 expression in CaSki cells and validated its effect on cell proliferation and invasion. Changes in gene expression were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or western blot. Down-regulation of ARL6IP1 expression by infection with ARL6IP1-specific RNAi-expressing vector inhibited CaSki cell proliferation and colony formation. In addition, down-regulation of ARL6IP1 expression arrested CaSki cell cycling at the G0/G1 phase and mitigated CaSki cell migration, determined by wound healing assays. ARL6I... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354786 Sun, 07 Mar 2010 00:00:00 +0100 3354786 http://www.medworm.com/index.php?rid=3354784&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213511%26dopt%3DAbstract Authors: Ciura J, Jagodziński PP The primary transcript of vascular endothelial growth factor (VEGF) can be alternatively spliced and translated to pro-angiogenic and anti-angiogenic VEGF variants. We investigated the effect of sodium butyrate (NaB) on pro-angiogenic and anti-angiogenic VEGF variants production in immortalized human lung microvascular endothelial cells (HLMEC). These cells were cultured in the absence or in the presence of NaB, followed by total RNA and protein isolation. The transcript and protein levels of pro-angiogenic and anti-angiogenic VEGF variants were evaluated by reverse transcription, real-time quantitative PCR and western blot analysis. We found that NaB significantly increased the anti-angiogenic transcript and protein levels of the VEGF 121b, VEGF1... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354784 Sun, 07 Mar 2010 00:00:00 +0100 3354784 http://www.medworm.com/index.php?rid=3354783&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213512%26dopt%3DAbstract Authors: Xia N, Zhang G, Liu XY, Deng L, Cai GL, Zhang Y, Wang XJ, Zhao J, Huang LL, Kang ZS Proteins encoded by the NAC gene family constitute one of the largest plant-specific transcription factors, which have been identified to play many important roles in both abiotic and biotic stress adaptation, as well as in plant development regulation. In the current paper, a full-length cDNA sequence of a novel wheat NAC gene, designated as TaNAC4, was isolated using in silico cloning and the reverse transcription PCR (RT-PCR) methods. TaNAC4 sharing high homology with rice OsNAC4 gene was predicted to encode a protein of 308 amino acid residues, which contained a plant-specific NAC domain in the N-terminus. Transient expression analysis indicated that the deduced TaNAC4 protein was localized... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354783 Sun, 07 Mar 2010 00:00:00 +0100 3354783 http://www.medworm.com/index.php?rid=3354782&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213513%26dopt%3DAbstract Authors: Subramanian MV, James TJ Aging, a multifactorial process of enormous complexity is characterised by physio-chemical and biological aspects of cellular functions. It is closely associated with changes in metabolism of various biological molecules in the system. In the present study, we have investigated the effect of deprenyl on cerebellum during ageing process in male Wistar rats with respect to the changes in levels of protein, glycoproteins and amino acids in experimental rats of three age groups (6, 12 and 18 months old). Intraperitoneal administration of liquid deprenyl (2 mg/kg body weight/day for a period of 15 days i.p., significantly P < 0.05) attenuated age-associated alterations in the levels of amino acids (taurine, aspartate, glutamate, arginine, hydroxy proline... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3354782 Sun, 07 Mar 2010 00:00:00 +0100 3354782 http://www.medworm.com/index.php?rid=3340000&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204523%26dopt%3DAbstract We report here the rapid procedure for globin chain analysis. The present method will be useful for the determination of globin chain analysis in clinical laboratories, as well as in thalassemia and sickle cell disease patients. PMID: 20204523 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3340000 Fri, 05 Mar 2010 00:00:00 +0100 3340000 http://www.medworm.com/index.php?rid=3339997&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204526%26dopt%3DAbstract In this study, we aimed to detect the single nucleotide polymorphisms (SNPs) of the chicken FATP1 gene and discern the potential association between FATP1 SNPs and chicken carcass traits. A total of 620 meat-type quality chickens from six commercial pure lines (S01, S02, S03, S05, S06 and D99) and two cross lines (S05 x S01 and S06 x S01) were screened by using the single-strand conformational polymorphism analysis (SSCP) and DNA sequencing. Five SNPs [g.49360G > A, g.48195G > A, g.46847A > G, g.46818A > G, and g.46555A > G] were identified in chicken FATP1 gene. SNP g.46818 A > G was a rare variant and was not considered in the subsequent analysis. Sixteen haplotypes were reconstructed on the basis of the other four SNPs. The linear regression model analysis indicated th... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339997 Fri, 05 Mar 2010 00:00:00 +0100 3339997 http://www.medworm.com/index.php?rid=3339999&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204524%26dopt%3DAbstract Authors: Gachomo EW, Seufferheld MJ, Kotchoni SO Previous studies have shown the role of melanized appressoria in the pathogenicity of various fungi. Diplocarpon rosae is a worldwide outdoor fungal pathogen of rose plants causing black spot disease of rose leaves. To fully understand how this fungus colonizes its host, which is critical for the development of an efficient and sustainable disease management program, we studied the fungal (especially the appressoria) structures of D. rosae in detail at an early stage of infection. Using both microscopic and biochemical analyses, we observed strong melanized appressoria formation localized at the point of D. rosae penetration, which forms the pathogen-plant interface. Treatment of infected plants with melanin biosynthesis inhibitors (MBIs... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339999 Thu, 04 Mar 2010 00:00:00 +0100 3339999 http://www.medworm.com/index.php?rid=3339998&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204525%26dopt%3DAbstract Authors: Giel-Pietraszuk M, Fedoruk-Wyszomirska A, Barciszewski J Formation and stabilization of RNA structure in the cell depends on its interaction with solvent and metal ions. High hydrostatic pressure (HHP) is a convenient tool in an analysis of the role of small molecules in the structure stabilization of biological macromolecules. Analysis of HHP effect and various concentrations of ions showed that water induce formation of the active ribozyme structure. So, it is clear that water is the driving force of conformational changes of nucleic acid. PMID: 20204525 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339998 Thu, 04 Mar 2010 00:00:00 +0100 3339998 http://www.medworm.com/index.php?rid=3339996&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204527%26dopt%3DAbstract Authors: Lee EH, Hyun DH, Park EH, Lim CJ In the present work, a second gene encoding protein disulfide isomerase (PDI2) was cloned and characterized from Schizosaccharomyces pombe, and its regulation was studied. The structural gene encoding PDI2 was amplified from the genomic DNA using PCR, and ligated into the E. coli-yeast shuttle vector pRS316 to generate the recombinant plasmid pYPDI2. The determined DNA sequence carries 2,578 bp and is able to encode a protein of 726 amino acid sequence with CGAC at the putative active site. The fission yeast cells harboring pYPDI2 contained 1.62- and 2.73-fold higher PDI activity than the control yeast cells in exponential and stationary phases, respectively, indicating that the cloned gene is in vivo functioning. The PDI2 mRNA levels in both v... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339996 Thu, 04 Mar 2010 00:00:00 +0100 3339996 http://www.medworm.com/index.php?rid=3339995&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204528%26dopt%3DAbstract In conclusion, GH1 expression driven by AAV2/1 in normal animals did not improve muscle strength but did increase muscle mass and may have systemic effects in healthy animals. PMID: 20204528 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339995 Thu, 04 Mar 2010 00:00:00 +0100 3339995 http://www.medworm.com/index.php?rid=3339994&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20204529%26dopt%3DAbstract Authors: Margam VM, Gachomo EW, Shukle JH, Ariyo OO, Seufferheld MJ, Kotchoni SO Genomic DNA extraction protocols generally require the use of expensive and hazardous reagents necessary for decontamination of phenolic compounds from the extracts. In addition, they are lengthy, hindering large-scale sample extractions necessary for high-throughput analyses. Here we describe a simple, time and cost-efficient method for genomic DNA extraction from insects. The extracted DNA was successfully used in a Polymerase Chain Reaction (PCR), making it suitable for automation for large-scale genetic analysis and barcoding studies. The protocol employs a single purification step to remove polysaccharides and other contaminating compounds using a non-hazardous reagent buffer. In addition, we conducte... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3339994 Thu, 04 Mar 2010 00:00:00 +0100 3339994 http://www.medworm.com/index.php?rid=3328094&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20195768%26dopt%3DAbstract Authors: Li XH, Wang D, Zhou F, Yang HJ, Bhaskar R, Hu JB, Sun CG, Miao YG Cellulases catalyze the hydrolysis of cellulose which are mainly three types: endoglucanases, cellobiohydrolases and beta-glucosidases. It can be used in converting cellulosic biomass to glucose that can be used in different applications such as production of fuel ethanol, animal feed, waste water treatment and in brewing industry. In this paper, we cloned a 1380-bp endoglucanase I (EG I) gene from mycelium of filamentous fungus Trichoderma viride strain AS 3.3711 using PCR-based exon splicing methods, and expressed the recombinant EG I mature peptide protein in both silkworm BmN cell line and silkworm larvae with a newly established Bac-to-Bac/BmNPV mutant baculovirus expression system, which lacks the virus-en... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3328094 Tue, 02 Mar 2010 00:00:00 +0100 3328094 http://www.medworm.com/index.php?rid=3328092&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20195770%26dopt%3DAbstract Authors: Liu Z, Yang X, Sun D, Song J, Chen G, Juba O, Yang Q Trichoderma asperellum, a filamentous soil fungus, is an effective biocontrol agent against many fungal plant pathogenic species. In the present study, we investigated the biological control properties of the strain T. asperellum T4. T. asperellum fermentation products significantly decreased the ability of Rhizoctonia solani and Sclerotinia sclerotiorum to infect rice and soybean, respectively. To further elucidate the biocontrol mechanisms of T. asperellum at the molecular level, a cDNA library was constructed from its mycelium. In total, 3114 expressed sequence tags (ESTs) were generated, which represented 1,554 unigenes, including 354 contigs and 1,200 singletons. Among these unigenes, 731 represented known genes while 8... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3328092 Tue, 02 Mar 2010 00:00:00 +0100 3328092 http://www.medworm.com/index.php?rid=3328095&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20195767%26dopt%3DAbstract Authors: Liu M, Shen S, Chen F, Yu W, Yu L The septin is a conserved GTP binding protein family which is involved in multiple cellular processes. Many evidences have indicated that some septins were abnormally expressed in certain kinds of tumors and the altered expressions were related to the process of carcinogenesis. To better understand the relationship between septins and cancer, we compared the expression of 14 human septin family members in 35 kinds of tumor types with their normal counterparts using the publicly available ONCOMINE microarray database. We found altered expression of most septin members in many kinds of tumors. Significantly, SEPT2, SEPT8, SEPT9, SEPT11 were consistently up-regulated, and SEPT4, SEPT10 were down-regulated in most cancer types investigated. Furthe... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3328095 Sat, 27 Feb 2010 00:00:00 +0100 3328095 http://www.medworm.com/index.php?rid=3328093&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20195769%26dopt%3DAbstract In this study blood samples were collected simultaneously from portal, arterial, peripheral venous and central venous compartments from patients undergoing laparotomy. ELISA and high sensitivity Bioplex was used to measure the concentration of acylated and des acyl ghrelin. We found median (95% confidence interval (CI)) plasma acylated ghrelin (pg/ml) was 35.8 (30.0-59.6) in the portal compartment compared to 51.5 (37.6-74.8; P < 0.05, n = 11) in the arterial, 39.3 (33.3-56.3) in the portal compartment compared to 55.0 (48.5-77.0; P < 0.001, n = 12) in the peripheral venous and 36.0 (33.1-57.4) in the portal compartment compared to 48.9 (43.3-65.6; P < 0.01, n = 15) in the central venous compartment. Median (95% CI) plasma des acyl ghrelin levels (pg/ml) was 173 (125-220) in the p... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3328093 Sat, 27 Feb 2010 00:00:00 +0100 3328093 http://www.medworm.com/index.php?rid=3319079&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191384%26dopt%3DAbstract Authors: Pang Y, Lei C, Zhang C, Lan X, Yue W, Gu C, Chen D, Wang J, Chen H PCR-SSCP and DNA sequencing methods were employed to screen the genetic variation of vascular endothelial growth factor (VEGF) gene in 675 individuals belonging to three Chinese indigenous cattle breeds including Qinchuan (QC), Jiaxian Red (JX) and Nanyang (NY) breed. Three new single nucleotide polymorphisms (SNPs) (g.6765T > C ss130456744, g.6860A > G ss130456745, g.6893T > C ss130456746) were found. One SNP (g.6765T > C) was detected in intron II of VEGF gene in all three breeds and the other two SNPs (g.6860A > G, g.6893T > C) were in exon III of VEGF gene only in NY breed. Among them, two synonymous mutations of exon III were identified: CCA (Pro) > CCG (Pro) at position 65th amino aci... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3319079 Sat, 27 Feb 2010 00:00:00 +0100 3319079 http://www.medworm.com/index.php?rid=3319078&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191385%26dopt%3DAbstract Authors: Qiu J, Cheng R, Zhou XY, Zhu JG, Zhu C, Qin DN, Kou CZ, Guo XR To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity using cDNA microarrays. Male Sprague-Dawley rats were fed either the HFD or the normal diet. Seven weeks later, the weights of obese models (362.92 +/- 39.65 g) were significantly higher than those of normal control rats (315.22 +/- 42.30 g, P < 0.01) and the wet weights of adipose tissue of rats fed with HFD (9.29 +/- 5.14 g) were significantly higher than those of normal control rats (4.09 +/- 2.69 g, P < 0.01), which confirmed the successful preparation of obese models. cDNA microarrays containing 9 216 genes/Ests wer... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3319078 Sat, 27 Feb 2010 00:00:00 +0100 3319078 http://www.medworm.com/index.php?rid=3319077&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191386%26dopt%3DAbstract Analyses of functional IL10 and TNF-alpha genotypes in Behçet's syndrome. Mol Biol Rep. 2010 Feb 27; Authors: Ateş O, Dalyan L, Hatemi G, Hamuryudan V, Topal-Sarıkaya A We aim to ascertain the possible involvement of functional IL10 and TNF-alpha promoter polymorphisms on the susceptibility to Behçet's syndrome (BS), to examine whether IL10 and TNF-alpha genotypes might work synergistically influencing susceptibility to BS. IL10 -1082G/A, -819C/T and -592C/A and TNF -308G/A polymorphisms were analyzed in 102 Turkish patients with BS and 102 healthy subjects by using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). We have found no significant associations between IL10 -1082G/A, -819C/T, -592C/A, TNF-alpha -308G/A polymorphisms... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3319077 Sat, 27 Feb 2010 00:00:00 +0100 3319077 http://www.medworm.com/index.php?rid=3315907&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182803%26dopt%3DAbstract Authors: Yuan FL, Li X, Lu WG, Li CW, Li JP, Wang Y The vacuolar ATPase (V-ATPase) is a multisubunit enzyme that couples ATP hydrolysis to proton pumping across membranes. Recently, there is increasing evidence that V-ATPase may contribute to the pathogenesis of bone resorption disorders due to it is predominantly expressed in osteoclasts also function in bone resorption making it a good candidate in a therapeutic target for osteoporosis. Osteoclasts are capable of generating an acidic microenvironment necessary for bone resorption by utilizing V-ATPases to pump protons into the resorption lacuna. In addition, it has been shown that therapeutic interventions have been proposed that specifically target inhibition of the osteoclast proton pump. Modulation of osteoclastic V-ATPase activit... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315907 Thu, 25 Feb 2010 00:00:00 +0100 3315907 http://www.medworm.com/index.php?rid=3315906&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182804%26dopt%3DAbstract This study was aimed to search genetic variants for the swine nerve growth factor gene that associated with regulation of proliferation and differentiation of nervous systems. The swine nerve growth factor gene was screened with 5 primer sets for random populations of crossbred pigs born 2005-2007 at National Institute of Animal Science (NIAS). To verify genetic variants of miniature pigs, a total of 288,000 BAC clones generated from NIAS in 2007 were used. The selection of primer sequences was based on sequences of the swine in GenBank (L31898), and genetic variants have been discovered in the crossbred population positioned at 381 (A/C), 412 (C/T), 422 (G/A), 468 (G/C), 496 (A/G), 538 (T/C), 540 (G/A), and 547 (A/G) showing substitutions of amino acids. The identified sequences of miniat... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315906 Thu, 25 Feb 2010 00:00:00 +0100 3315906 http://www.medworm.com/index.php?rid=3315905&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182805%26dopt%3DAbstract In conclusion, our data have suggested that genetic variant of CCR5 might be associated with the development of MI. Further larger sample size studies are required to confirm our findings. PMID: 20182805 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315905 Thu, 25 Feb 2010 00:00:00 +0100 3315905 http://www.medworm.com/index.php?rid=3315904&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182806%26dopt%3DAbstract In this study, two novel SNPs (EU743939:g.5174T>C in intron 4 and EU743939:g.8350C>A in intron 7) in TNNI1 and one SNP (EU696779:g.1167C>T in intron 3) in TNNI2 were identified by PCR-RFLP (PCR restriction fragment length polymorphism) using XbaI, MspI and SmaI restriction enzyme, respectively. The allele frequencies of three novel SNPs were determined in the genetically diverse pig breeds including ten Chinese indigenous pigs and three Western commercial pig breeds. Association analysis of the SNPs with the carcass traits were conducted in a Large White x Meishan F(2) pig population. The linkage of two SNPs (g.5174T>C and g.8350C>A) in TNNI1 gene had significant effect on fat percentage. Besides these, the g.5174T>C polymorphism was also significantly associated with ski... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315904 Thu, 25 Feb 2010 00:00:00 +0100 3315904 http://www.medworm.com/index.php?rid=3315903&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182807%26dopt%3DAbstract In this study we describe a standardized fluorescent multiplex PCR combined with computerized analysis, which allows rapid and accurate analysis of a high number of samples and obviates the need to compare tumors with matching normal tissue. PMID: 20182807 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315903 Thu, 25 Feb 2010 00:00:00 +0100 3315903 http://www.medworm.com/index.php?rid=3315914&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20177792%26dopt%3DAbstract Authors: Li Y, Tang K, Zhang Z, Zhang M, Zeng Z, He Z, He L, Wan C In the past decade, a number of case-control studies have been carried out to investigate the relationship between the ApoE polymorphism and diabetic nephropathy. However, the results have been inconclusive. To investigate this inconsistency, we performed a meta-analysis of all available studies dealing with the relationship between the ApoE polymorphism and DN. The 23 studies in the meta- analysis included 6,012 diabetic patients with (n = 2,979) and without (n = 3,033) DN. The ApoE epsilon2 allele was significantly associated with DN (OR = 1.64, 95% CI: 1.26-2.13; P(Z) = 0.00027), whereas the epsilon4 allele was non-significantly associated with DN (OR = 0.93, 95% CI: 0.78-1.11; P(Z) = 0.418). However, significant het... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315914 Wed, 24 Feb 2010 00:00:00 +0100 3315914 http://www.medworm.com/index.php?rid=3315912&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20180024%26dopt%3DAbstract Authors: Kim H, Lee S, Park B, Che L, Lee S Cancer-upregulated gene 2 (CUG2), which was named since it was originally identified as a putative oncogene up-regulated in various human cancers, was recently renamed CENP-W based on the new findings that it is a component of the centromeric complex playing a crucial role in the assembly of functional kinetochore complex during mitosis. To understand the transcriptional regulation of CENP-W, we analyzed its TATA-less promoter and identified a GC-rich putative Sp1 binding site located at -46 to -36 that was critical in CENP-W expression. Competitive electrophoretic gel mobility shift assay using mutated oligos and supershift assays with Sp1 and Sp3 antibodies demonstrated that both proteins specifically bound to this promoter region. Moreover... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315912 Wed, 24 Feb 2010 00:00:00 +0100 3315912 http://www.medworm.com/index.php?rid=3315910&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20180025%26dopt%3DAbstract Authors: Cho IS, Kim J, Seo HY, Lim DH, Hong JS, Park YH, Park DC, Hong KC, Whang KY, Lee YS MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously i... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315910 Wed, 24 Feb 2010 00:00:00 +0100 3315910 http://www.medworm.com/index.php?rid=3315909&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182801%26dopt%3DAbstract Authors: Miao Y, Qiu Y, Lin Y, Miao Z, Zhang J, Lu X Pyruvate, an endogenous metabolite of glycolysis, is an anti-toxicity agent. Recent studies have suggested possible roles for pyruvate in protecting CNS neurons from excitotoxic and metabolic insults. Utilizing cultures derived from embryonic rat cortex, the studies presented in this paper indicate that an astroglia-mediated mechanism is involved in the neuroprotective effects of pyruvate against glutamate toxicity. Glutamate-induced toxicity could be reversed by pyruvate in a mixed culture of cortex cells. Importantly, in pure neuronal cultures from the same tissue, pyruvate failed to protect against glutamate toxicity. Addition of astroglia to the pure neuronal cultures restores the ability of pyruvate to protect neurons from gluta... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315909 Wed, 24 Feb 2010 00:00:00 +0100 3315909 http://www.medworm.com/index.php?rid=3315908&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20182802%26dopt%3DAbstract This study was to examine the effects of over-expression of the full-length MiARF2 open reading frame on the root and hypocotyl growth in Arabidopsis. Phenotype analysis showed that the T(3) transgenic lines had about 20-30% reduction in the length of hypocotyls and roots of the seedlings in comparison with the wild-type. The transcription levels of ANT and ARGOS genes which play a role in controlling organ size and cell proliferation in the transgenic seedlings also decreased. Therefore, the inhibited root and hypocotyl growth in the transgenic seedlings may be associated with the down-regulated transcription of ANT and ARGOS by the over-expression of MiARF2. This study also suggests that although MiARF2 only has a single DNA-binding domain (DBD), it can function as other ARF-like protein... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315908 Wed, 24 Feb 2010 00:00:00 +0100 3315908 http://www.medworm.com/index.php?rid=3315913&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20177793%26dopt%3DAbstract In conclusion, familial gastric carcinoma families in China carrying the T1151A polymorphism may have a higher risk of suffering from gastric cancer. This gene polymorphism can be used as a candidate screening index for high-risk populations. PMID: 20177793 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315913 Tue, 23 Feb 2010 00:00:00 +0100 3315913 http://www.medworm.com/index.php?rid=3315915&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20174991%26dopt%3DAbstract Authors: Wang TY, Han ZM, Chai YR, Zhang JH Genomic DNA encompasses several levels of organization, the nuclear matrix mediates the formation of DNA loop domains that are anchored to matrix attachment regions (MARs). By means of specific interaction with MAR binding proteins (MARBPs), MAR plays an important regulation role in enhancing transgene expression, decreasing expression variation among individuals of different transformants and serving as the replication origin. Through these years, some MARBPs have been identified and characterized from humans, plants, animals and algae so far and the list is growing. Most of MARBPs exist in a co-repressor/co-activator complex and involve in chromosome folding, regulation of gene expression, influencing cell development and inducing cell apop... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315915 Mon, 22 Feb 2010 00:00:00 +0100 3315915 http://www.medworm.com/index.php?rid=3315917&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20174873%26dopt%3DAbstract Authors: Kim YI, Lee JW, Lee MH, Park SW, Cho BN, Lee HK 15-deoxy-(12,14)-prostaglandin J(2) (15d-PGJ(2)) is an anti-inflammatory prostaglandin that plays a role in promoting the resolution of inflammation. We investigated the effects of 15d-PGJ(2) on the production of IL-8 and on the expression of Toll-like receptors (TLRs) 2 in human primary keratinocytes stimulated with lipopolysaccharide (LPS). Cell proliferation was analyzed using the MTT assay, TLR2 and -4 mRNA expression was detected by RT-PCR, and IL-8 production and NF-kappaB p65 activities were determined by ELISA. LPS and 15d-PGJ(2) did not influence the proliferation rate at low concentrations (0.5 and 2.0 muM) in keratinocytes, and showed toxicity at high concentrations (5.0 muM). LPS, compared with control, induced the ex... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315917 Sun, 21 Feb 2010 00:00:00 +0100 3315917 http://www.medworm.com/index.php?rid=3315916&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20174874%26dopt%3DAbstract Authors: Hu ZQ, Zhang JY, Ji CN, Xie Y, Chen JZ, Mao YM Bim is a proapoptotic member of the Bcl-2 family and is primarily involved in the regulation of the intrinsic apoptotic pathway. However, the detail of regulation of Bim's proapoptotic activity has not been clarified yet. Using Bim L as bait, we screened a human fetal cDNA library for interacting proteins and identified Grb10 as an interactor. This interaction was verified by co-immunoprecipitation and intracellular co-localization studies. The potential segment of Bim L that binds Grb10 was identified via a yeast mating test. Grb10 interacted with the DBD (dynein binding domain) of Bim and inhibited apoptosis triggered by overexpression of DBD containing Bim isoforms. The putative phosphorylation sites on DBD of Bim play a role f... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3315916 Sat, 20 Feb 2010 00:00:00 +0100 3315916 http://www.medworm.com/index.php?rid=3295021&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20165923%26dopt%3DAbstract Authors: Ozdemir O, Sezgin I, Kurtulgan HK, Candan F, Koksal B, Sumer H, Icagasioglu D, Uslu A, Yildiz F, Arslan S, Cetinkaya S, Citli S, Oztemur Z, Kayatas M The Familial Mediterranean Fever (FMF) shows an autosomal recessive pattern of inheritance and affects certain ethnic groups. Disease is caused by mutations in MEFV gene and more than 180 mutations have been defined in affected individuals. Current study aimed to determine the frequency-type of the mutations for MEFV gene in Sivas-middle Anatolian city. The cohort was composed of 3340 patients. MEFV gene mutations were studied by multiplex PCR based reverse hybridization stripAssay method. Patients' clinical features were; family history: 68%, erysipelas-like erythema: 17.6%, fever: 89.9%, abdominal pain: 84.2%, peritonitis: 90.2... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3295021 Thu, 18 Feb 2010 00:00:00 +0100 3295021 http://www.medworm.com/index.php?rid=3269589&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151325%26dopt%3DAbstract Authors: Mao X, Zeng X, Wang J, Qiao S Leptin plays a critical role in regulating muscle protein metabolism by binding with leptin receptors in a 1:1 stoichiometry. However, the role for leucine in the regulation of leptin receptor expression in muscle has not been investigated. The present study was conducted to test the hypothesis that leucine regulates leptin receptor levels in C2C12 myotubes. Cells were cultured in the presence of DMEM/F12 medium containing supplemental 0 or 5 mM L: -leucine. Leptin receptor expression by C2C12 myotubes peaked at 2 h post-supplementation. Additionally, leucine stimulated leptin receptor expression at both mRNA and protein levels in a dose-dependent manner. Furthermore, leucine enhanced the phosphorylation of mammalian target of rapamycin (mTOR). Ad... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3269589 Fri, 12 Feb 2010 00:00:00 +0100 3269589 http://www.medworm.com/index.php?rid=3269588&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151326%26dopt%3DAbstract Authors: Jin M, Wang L, Li S, Xing MX, Zhang X Keratin-associated protein is one of the major structural proteins of the hair, whose content in hair has important effect on the quality of cashmere. In order to study the relationship between HGTKAP gene expression and cashmere fineness, the quantitative real-time RT-PCR (qRT-PCR) was firstly used to detect the levels of KAP7.1, KAP8.2 gene expression in the primary and secondary hair follicles; semi-quantitative RT-PCR was used to detect whether KAP7.1, KAP8.2 gene are expressed in heart, liver, spleen, lung, kidney tissues; and in situ hybridization(ISH) to detect KAP7.1 gene expression location. qRT-PCR result showed that the expression of both KAP7.1 and KAP8.2 gene in the secondary hair follicles are significantly higher than that i... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3269588 Fri, 12 Feb 2010 00:00:00 +0100 3269588 http://www.medworm.com/index.php?rid=3263073&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20146005%26dopt%3DAbstract In conclusion, miR-221 is important in tumorigenesis of HCC, possibly by specifically down-regulating p27(Kip1), a cell-cycle inhibitor. These results indicate miR-221 as a new therapeutic target in HCC. PMID: 20146005 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3263073 Wed, 10 Feb 2010 00:00:00 +0100 3263073 http://www.medworm.com/index.php?rid=3263072&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20146006%26dopt%3DAbstract Authors: Falcón-Ramírez E, Casas-Avila L, Miranda A, Diez P, Castro C, Rubio J, Gómez R, Valdés-Flores M The Sp1 binding site polymorphism in collagen type I alpha 1 gene (COLIA1) has been associated with osteoporosis (OP) and bone mineral density (BMD). The aim of this study was to explore the association of this polymorphism with OP and BMD in the Mexican population by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) procedure. Allelic and genotypic frequencies from the Sp1 polymorphism were determined in 100 women with OP, 100 women without OP and 500 subjects from general Mexican population (GMP). Distribution of Sp1 polymorphism was in Hardy-Weinberg equilibrium. In spite of population structure due to racial mix in Mexican popu... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3263072 Wed, 10 Feb 2010 00:00:00 +0100 3263072 http://www.medworm.com/index.php?rid=3255136&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140706%26dopt%3DAbstract In this study, combining a computational method based on expressed sequence tag (EST) analysis with experimental identification based on small RNA cDNA library, we identified 31 miRNAs belong to 24 families in sheep, 2 of which were novel miRNAs which had never been previously identified in any species. Especially, we cloned 12 miRNAs from the sheep skeletal muscle, which were good candidate miRNAs to be studied about the miRNA-dependant regulated process of muscle development, and we identified four pairs of miRNA/miRNA* and one pair of miRNA-3p/miRNA-5p from sheep EST sequences. Expression analysis indicated that some miRNAs were expressed in a specific tissue, and the pair of miRNA-3p/miRNA-5p and one pair of miRNA/miRNA* had a similar relative expression pattern in some tissues, respec... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255136 Sun, 07 Feb 2010 00:00:00 +0100 3255136 http://www.medworm.com/index.php?rid=3255135&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140707%26dopt%3DAbstract Authors: Sun J, Jin Q, Zhang C, Fang X, Gu C, Lei C, Wang J, Chen H Transgenically expressed Syndecan-1 was found in the hypothalamic nuclei that control energy balance, and was associated with maturity-onset obesity, while ghrelin has been shown to play important roles in the control of food intake, gastric acid secretion, energy homeostasis, and glucose and lipid metabolism. However, the roles of genetic variations of Syndecan-1 and ghrelin on growth trait have few been reported in cattle. Herein, five Chinese cattle breeds were analyzed by PCR-SSCP and DNA sequencing methods. The bovine ghrelin gene showed eleven SNPs g.[267G>A, 271G>A, 290C>T, 326A>G, 327T>C, 420C>A, 569A>G, 945C>T, 993C>T, 4491A>G, 4644G>A] and three SNPs g.[420C>A, 569 A>G, ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255135 Sun, 07 Feb 2010 00:00:00 +0100 3255135 http://www.medworm.com/index.php?rid=3255134&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140708%26dopt%3DAbstract In this study, we investigated polymorphisms within the caprine SST and SSTR1 genes and determined their relationship with growth traits. As there were no sequence information of the caprine SST and SSTR1 genes, we explored their DNA sequence and genomic organizations. The caprine SST gene is organized in two exons and is transcribed into an mRNA containing 351 bp of sequence coding for a protein of 116 amino acids. Its protein sequences showed substantial similarity (97-99%) to its respective orthologs from cattle, human and mouse. We also cloned and sequenced a 1.2 kb DNA fragment which contained the major part of the coding region and 3' UTR of the caprine SSTR1 gene. We then detected the polymorphisms in these determined sequences by PCR-SSCP and DNA sequencing methods in 459 goats fro... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255134 Sun, 07 Feb 2010 00:00:00 +0100 3255134 http://www.medworm.com/index.php?rid=3255133&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140709%26dopt%3DAbstract Authors: Taliercio E ADP-glucose pyrophosphorylase (ADPGp, EC 2.7.7.27) is a tetrameric protein composed of two small and two large subunits that catalyzes the biosynthesis of ADP-glucose from glucose-phosphate which is used to provide the glucose subunits for starch biosynthesis. A second cotton gene encoding an ADPGp small subunit has been cloned and characterized. The gene contains eight introns similar to previously reported potato and cotton ADPGp small subunit genes. The deduced translation of the gene contained a poorly conserved transit peptide and well conserved catalytic and regulatory elements typical of other plant ADPGps. The 5' end of the mRNA was cloned and sequenced to identify the transcriptional start site (TSS). The promoter region upstream of the TSS did not contain... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255133 Sun, 07 Feb 2010 00:00:00 +0100 3255133 http://www.medworm.com/index.php?rid=3255132&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140710%26dopt%3DAbstract Authors: Yue W, Fang X, Zhang C, Pang Y, Xu H, Gu C, Shao R, Lei C, Chen H The ATP-binding cassette transporter ABCG2 (also known as breast cancer resistance protein, BCRP) belongs to the ATP-binding cassette (ABC) family of transmembrane drug transporters, playing a crucial role in the protection of various cells and tissues against xenotoxins and/or endotoxins. Recently, several studies have proposed it as the potential gene underlying the QTL on bovine chromosome 6. Hence, in this study, the PCR-SSCP method was applied to detect two polymorphisms (A --> C and A --> G) in the target sequence coding nucleotide-binding domain (NBD) region of ABCG2 and evaluate its associations with milk production traits and mastitis-related traits among Chinese Holsteins. In the analyzed populat... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255132 Sun, 07 Feb 2010 00:00:00 +0100 3255132 http://www.medworm.com/index.php?rid=3255137&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140648%26dopt%3DAbstract Authors: Qin X, Gao F, Zhang J, Gao J, Lin S, Wang Y, Jiang L, Liao Y, Wang L, Jia Y, Tang L, Xu Y, Chen F A cDNA encoding translationally controlled tumor protein (TCTP) of Jatropha curcas L., JcTCTP, was isolated from an endosperm cDNA library. JcTCTP consisted of a 5' untranslated region (UTR) of 526 bp, a 3' UTR of 377 bp and an open reading frame (ORF) of 507 bp, encoding a protein of 168 amino acid residues, which contained two signature sequences of TCTP family. Its deduced amino acid sequence was similar to the other known plants TCTPs in a range of 77.4-92.3%. Expression of JcTCTP was the highest in the stem, endosperm at embryo formation stage and embryo of J. curcas tissues, and the lowest in the endosperm at seminal leaf embryo stage and flower, demonstrating a pattern of t... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3255137 Sat, 06 Feb 2010 00:00:00 +0100 3255137 http://www.medworm.com/index.php?rid=3248932&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20135232%26dopt%3DAbstract Authors: Yuan HM, Li KL, Ni RJ, Guo WD, Shen Z, Yang CP, Wang BC, Liu GF, Guo CH, Jiang J The chloroplast is one of the most important organelles in plants. Proteomic investigations of chloroplasts have been undertaken for many herb plant species, but to date no such investigation has been reported for woody plant chloroplasts. In the present study we initiated a systematic proteomic study of Populus chloroplasts using a shotgun proteomic method. After isolation of chloroplasts and tryptic digestion of the proteins, the protein fragments were separated via HPLC using an SCX column, and the peptides were analyzed by LC-MS/MS; 119 proteins were successfully identified. Based on annotation information in the UniProtKB/Swiss-Prot database, these proteins were identified as being localized ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3248932 Fri, 05 Feb 2010 00:00:00 +0100 3248932 http://www.medworm.com/index.php?rid=3244714&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127518%26dopt%3DAbstract Authors: Yang J, Zhang XD, Yang J, Ding JW, Liu ZQ, Li SG, Yang R To determine whether the cardioprotection effect of fluvastatin mediates by toll-like receptor 4 (TLR4) signaling pathway, fifty Sprague-Dawley rats were randomly divided into five groups: sham operation group, ischemia/reperfusion (I/R) group, fluvastatin groups (high-dosage, medium-dosage, low-dosage, n = 10 in each group). Except sham operation group, the rest four groups of rats were artificially afflicted with coronary occlusion for 30 min, then reperfusion 2 h. Light microscope and transmission electronic microscope were used to observe structural changes of myocardium. RT-PCR was used to measure TLR4 mRNA expression level, TLR4 protein expression was detected by immunohistochemistry. Western blot was used to measu... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244714 Thu, 04 Feb 2010 00:00:00 +0100 3244714 http://www.medworm.com/index.php?rid=3244709&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20131008%26dopt%3DAbstract In conclusion, we elucidated and characterized the porcine alpha-gal A gene and enzyme. Similarity in enzymatic profile and chromosomal location between alpha-gal A of porcine and human origins may be of great advantage for the development of a large animal model for Fabry disease. PMID: 20131008 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244709 Thu, 04 Feb 2010 00:00:00 +0100 3244709 http://www.medworm.com/index.php?rid=3244708&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20131009%26dopt%3DAbstract Authors: He X, Gao H, Liu C, Fan B, Liu B The Wingless-type MMTV integration site (Wnt) family encodes secreted glycoproteins that are ligands for the frizzled family of seven-transmembrane receptors and the low density lipoprotein receptor-related protein family of co-receptors. The WNT10B gene inhibits differentiation of preadipocytes in vitro and impairs adipose development in vivo. In the present study, a 1,615-bp cDNA sequence of the porcine WNT10B gene was obtained by RT-PCR. The porcine WNT10B gene was assigned to 5p11-p15 by using the somatic cell hybrid panel (SCHP) and the radiation hybrid (IMpRH) panel. One SNP in the 3'-untranslated region (3'-UTR) was found and association analysis suggested that the SNP was associated with backfat thickness. Semi-quantitative RT-PCR showe... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244708 Thu, 04 Feb 2010 00:00:00 +0100 3244708 http://www.medworm.com/index.php?rid=3244707&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20131010%26dopt%3DAbstract Authors: Xu K, Kanno M, Yu H, Li Q, Kijima A The complete mitochondrial genome of Zhikong scallop Chlamys farreri is 21,695 bp in length and contains 12 protein-coding genes (the atp8 gene is absent, as in most bivalves), 2 ribosomal RNA genes, and 22 transfer RNA genes. The heavy strand has an overall A+T content of 58.7%. GC and AT skews for the mt genome of C. farreri are 0.337 and -0.184, respectively, indicating the nucleotide bias against C and A. The mitochondrial gene order of C. farreri differs drastically from the scallops Argopecten irradians, Mimachlamys nobilis and Placopecten magellanicus, which belong to the same family Pectinidae. 6623 bp non-coding nucleotides exist intergenically in the mitogenome of C. farreri, with a large continuous sequence (4763 bp) between tRNA ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244707 Thu, 04 Feb 2010 00:00:00 +0100 3244707 http://www.medworm.com/index.php?rid=3244706&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20131011%26dopt%3DAbstract In this study, an hsp90 gene was isolated from disc abalone (Haliotis discus). The complete nucleotide sequence of the hsp90 gene contains an open reading frame of 2,184 base pairs, encoding an 84 kDa protein. Disk abalone hsp90 shares high sequence similarity with other hsp90 family proteins. Although the phylogenetic analysis did not classify it into the hsp90alpha group, the inductivity of this gene was confirmed by heat shock and lipopolysaccharide (LPS) challenge test. Disk abalone hsp90 gene displayed a rapid and reversible induction response to both an exposure of typical heat shock and the LPS challenge. Once given the sublethal heat shock treatment, the transcription of disk abalone hsp90 gene was significantly up-regulated. With a recovery of 12 h, the transcription of disk abalo... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244706 Thu, 04 Feb 2010 00:00:00 +0100 3244706 http://www.medworm.com/index.php?rid=3244718&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127514%26dopt%3DAbstract Authors: Zhang X, Shen W, Lu Y, Zheng X, Xue R, Cao G, Pan Z, Gong C For mass production of urease B subunit (UreB) and heat shock protein A subunit (HspA) of Helicobacter pylori with Bombyx mori nuclear polyhedrosis virus (BmNPV) baculovirus expression system (BES) and to determine whether they could be used as an oral vaccine against H. pylori, besides, to determine the time course of expressed recombinant protein and the optimum acquisition time directly through green fluorescence, HspA and enhanced green fluorescence protein (EGFP) genes were cloned into vector pFastBacDual to form donor vector pFastBacDual-(EGFP) (HspA), UreB gene was cloned into vector pFastBacDual to form donor vector pFastBacDual-UreB,then they were transformed into E. coli BmDH10Bac to obtain the recombinant B... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244718 Wed, 03 Feb 2010 00:00:00 +0100 3244718 http://www.medworm.com/index.php?rid=3244717&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127515%26dopt%3DAbstract Authors: Deng W, Zhou L, Zhou Y, Wang Y, Wang M, Zhao Y Three coding region cDNAs of duplicated PISTILLATA-like (PI-like) MADS-box genes, BnPI-1, BnPI-2 and BnPI-3, were isolated from B. napus by RT-PCR. The sequence analysis showed that the three PI cDNAs possessed 627, 627 and 625 nucleotides, respectively, and their nucleotide sequences had 96.49-98.72% similarity. Due to a deletion of two nucleotides, the protein sequence in the downstream of the frameshift site was altered in BnPI-3. Therefore, there were only 171 amino acids coded by BnPI-3, while there were 208 ones coded by BnPI-1 or BnPI-2. The deduced amino acid identity between BnPI-1 and BnPI-2 was 97.6% and the amino acid sequence of BnPI-1 and BnPI-2 shared 72.6% identity with BnPI-3. The deduced amino acid sequences of t... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244717 Wed, 03 Feb 2010 00:00:00 +0100 3244717 http://www.medworm.com/index.php?rid=3244716&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127516%26dopt%3DAbstract Authors: Chen G, Qiao Y, Yao J, Jiang Q, Lin X, Chen F, Lin F, Lin M, Lin L, Zhu P To construct a recombinant adenovirus vector expressing a RNAi for the Nuclear Factor kappa B (NF-kappaB)/p65 gene and use it to explore the role of the NF-kappaB pathway on the regulation of proliferation and apoptosis of vascular endothelial cells. A recombinant adenovirus containing a RNAi cassette targeting the p65 gene was constructed, and its silencing effect on p65 was detected by Western blot analysis in ECV304 cells. Expression of the p65 protein in ECV304 cells was efficiently down-regulated by the RNAi adenovirus for more than 6 days. ECV304 cells proliferation and apoptosis were measured using the MTT assay and flow cytometry, respectively. Blocking the NF-kappaB pathway with the RNAi adenovi... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244716 Wed, 03 Feb 2010 00:00:00 +0100 3244716 http://www.medworm.com/index.php?rid=3244715&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127517%26dopt%3DAbstract Authors: Zhang YQ, Li KN, Cui JH, Liu YF, Yang SJ Human nuclear respiratory factor 2 alpha subunit (NRF-2alpha) is fundamentally important to cell function and the development. We aimed to establish the monoclonal antibody (MAb) against the human NRF-2alpha protein and to investigate its distribution in human hepatocellular carcinoma (HCC) and tumor-adjacent tissues. The 6x His-NRF-2alpha fusion protein was successfully induced and purified. One monoclonal antibody (MAb) against human NRF-2alpha, 1-D10-E1-B11-G3 (IgG1), effective in detecting the recombinant and the cellular protein, was characterized. Using immunohistochemical analysis, the expression of NRF-2alpha was investigated in 38 cases of HCC specimens and 14 cases of tumor-adjacent specimens. Staining was found positive in 9 ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244715 Wed, 03 Feb 2010 00:00:00 +0100 3244715 http://www.medworm.com/index.php?rid=3244713&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127519%26dopt%3DAbstract Authors: Maghdooni Bagheri P, Govaerts I, De Ley M Metallothioneins (MTs), a group of small, cystein-rich proteins, possess various functions, including metal detoxification and homeostasis. We here report new findings on the participation of MT in cellular differentiation processes. MT isogene transcription was significantly increased in phorbol-12-myristate-13-acetate (PMA) incubated leukemic DAMI cells, which supports its role in cellular differentiation. To further address this possibility, we constructed one stable MT-2A overexpressing DAMI cell line. Increase of cell size, intracellular granulation and megakaryocytic specific antigen expression such as CD41 and CD42, and arresting cell proliferation have validated the role of MT in differentiation in this cell line. PMID: 201... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244713 Wed, 03 Feb 2010 00:00:00 +0100 3244713 http://www.medworm.com/index.php?rid=3244712&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127520%26dopt%3DAbstract Authors: Zhang HF, Wang JF, Wang Y, Zhu LG, Lei L The complement factor H (CFH) Y402H (T1277C) gene polymorphism has been reported to be associated with coronary heart disease (CHD), but results were conflicting. To evaluate the role of the variant in CHD, we performed meta-analyses of all available data. Both electronic and manual searches were performed, all relevant studies were identified. ORs with 95% confidential intervals (CI) under codominant (CC versus TT, TC versus TT), dominant (CC + TC versus TT) and recessive (CC versus TT + TC) models were calculated. Publication bias was addressed. Ten studies including 11 cohorts comprising of 29,764 participants were included. No association between the CFH T1227C polymorphism and CHD could be found. (For overall analysis: dominant mod... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244712 Wed, 03 Feb 2010 00:00:00 +0100 3244712 http://www.medworm.com/index.php?rid=3244711&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127521%26dopt%3DAbstract Authors: Khurana J, Singh R, Kaur J A lip gene from a Bacillus isolate was cloned and expressed in E. coli. By thermal denaturation analysis, T(1/2) of lipase was observed to be 7 min at 50 degrees C with less than 10% activity after 1 h incubation at 50 degrees C. To expand the functionality of cloned lipase, attempts have been made to create thermostable variants of lip gene. A lipase variant with an isoleucine to threonine amino acid substitution at the protein surface was isolated that demonstrated higher thermostability than its wild type predecessor. To explore the structure-function relationship, the lip gene product of wild type (WT) and mutant was characterized in detail. The mutation enhanced the specific activity of enzyme by 2-folds when compared with WT. The mutant enzyme ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244711 Wed, 03 Feb 2010 00:00:00 +0100 3244711 http://www.medworm.com/index.php?rid=3244710&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127522%26dopt%3DAbstract In this study, a full-length cDNA of the porcine SRPK3 gene encoding a 566 amino acid protein was isolated. It contains 14 exons over approximately 4.3 kb. The deduced amino acid sequence of porcine SRPK3 contains a bipartite kinase domain, and shows high similarities to their corresponding human and cattle homologues. Tissue distribution analysis indicated that porcine SRPK3 mRNAs are highly expressed in heart and skeletal muscle especially in uterus and parorchis, but at low level in brain, stomach, small intestine, and ovary. Expression pattern of SRPK3 was similar in Large White and Chinese Meishan breeds. Both the two breeds had the highest expression levels at fetal 65 days (P < 0.01), and decreased while the age increased until 60 days old, then increased at 120 days (P < 0.01... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244710 Wed, 03 Feb 2010 00:00:00 +0100 3244710 http://www.medworm.com/index.php?rid=3244725&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127176%26dopt%3DAbstract In conclusion, detection of BCR-ABL fusion gene expression in CML using Roche t (9; 22) kit has great clinical value in the primary diagnosis, monitoring treatment responses, and identification of relapse in CML patients. PMID: 20127176 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244725 Tue, 02 Feb 2010 00:00:00 +0100 3244725 http://www.medworm.com/index.php?rid=3244724&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127177%26dopt%3DAbstract Authors: De Luca V, Capasso C, Capasso A, Pastore M, Carginale V Phytoplasmas are small (0.2-0.8 mum), wall-less, pleiomorphic prokaryotes responsible of numerous economically important plant diseases. They are characterized by a very small genome and are obligate parasites of phloem tissues and some insects that act as vectors of infection. To investigate molecular mechanisms involved in pathogenesis, the differential display technique was here applied to identify plant genes whose transcription was significantly altered in leaves of Madagascar periwinkle (Catharanthus roseus (L.) G.Don) infected by 'Candidatus Phytoplasma pyri'. We detected, reamplified, cloned, and sequenced 16 putative differentially expressed cDNA fragments. Northern blot analysis revealed that seven of the 16 gen... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244724 Tue, 02 Feb 2010 00:00:00 +0100 3244724 http://www.medworm.com/index.php?rid=3244723&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127178%26dopt%3DAbstract In this study, we validated and refined gene structure, screened for mRNA splice variants and analyzed the tissue-specific gene expression patterns of both loci as a prerequisite to elucidate their potential physiological function. Based on comparative sequence analysis, a new full-length gene model for the bovine IRAK1 gene was developed and confirmed experimentally. Expression of IRAK1 mRNA was found in a variety of tissues, and a splice variant was identified in skeletal muscle caused by an in-frame deleted segment of 210 bp affecting regions of intrinsic disorder in the respective protein. For the locus LOC618944, our data contributed to a revised gene model and its assignment to BTA23 (bovine chromosome 23) on the current bovine genome assembly supported by comparative similarity anal... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244723 Tue, 02 Feb 2010 00:00:00 +0100 3244723 http://www.medworm.com/index.php?rid=3244722&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127179%26dopt%3DAbstract Authors: Saini A, Dua A, Mohindra V, Lakra WS Bagrid catfishes constitute a very important group of fishes having immense commercial importance in south-east countries. The phylogenetic relationships and genome specificity among six species of Bagrid catfishes (Mystus bleekeri, M. cavasius, M. vittatus, M. tengara, M. aor and M. seenghala) were investigated using RAPD markers as discriminating characters for the first time. 511 RAPD fragments were generated using ten decamer primers of arbitrary nucleotide sequences. Amplification reactions resulted in fragments ranging in length between 92 and 2,863 bp, which were assigned to 155 RAPD loci. Clearly resolved and repeatable bands were scored for their presence or absence in a binary matrix. Different RAPD profiles were observed for all ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244722 Tue, 02 Feb 2010 00:00:00 +0100 3244722 http://www.medworm.com/index.php?rid=3244721&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127180%26dopt%3DAbstract Authors: Sobti RC, Berhane N, Mahdi SA, Kler R, Hosseini SA, Kuttiat V, Wanchu A HIV/AIDS remains to be one of the killing diseases of mankind. Host genetic response is one of the factor which determine susceptibility to HIV and disease progression to AIDS. The aim of the present study was to evaluate the impact of ERCC2 Lyc ( 751 ) Gln (excision repair cross complementing rodent repair deficiency, complementation group 2) polymorphism on HIV-1 susceptibility and disease progression to AIDS, as this gene has been reported to intervene in degrading retroviral cDNA before it integrates with the host DNA. This case control study included 300 HIV seropositive cases and an equal number of HIV seronegative controls. DNA was isolated from the blood samples of study subjects and genotyping of ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244721 Tue, 02 Feb 2010 00:00:00 +0100 3244721 http://www.medworm.com/index.php?rid=3244720&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127181%26dopt%3DAbstract In this study, an association of MDR1 gene polymorphism and the occurrence of colorectal cancer were evaluated. In this case-control-designed 118 unrelated colorectal cancer and 137 sex-and-ages matched healthy controls were enrolled. The C3435T MDR1 gene polymorphism was identified using the polymerase chain reaction-restriction fragment length polymorphism method. Significantly increased frequencies of the 3435T allele and the 3435TT were observed in patients with colorectal cancer compared with controls (P = 0.03; OR, 95% CI; 1.46 for 3435T allele and P = 0.003; OR, 95% CI; 2.2 for 3435TT genotype). In contrast, frequency of genotype TT was significantly higher in controls compared to colorectal cancer (P = 0.006; OR, 95% CI; 0.49 for TC genotype). In this study suggest that C3435T MDR1... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244720 Tue, 02 Feb 2010 00:00:00 +0100 3244720 http://www.medworm.com/index.php?rid=3244719&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127182%26dopt%3DAbstract Authors: Karahalil B, Emerce E, Kocabaş NA, Akkaş E The presenting study was investigated the associations between individual susceptibility and cigarette smoke on maternal chromosomal damage and neonatal birth growth in smoking mothers since little known about genetic susceptibility to cigarette smoke in relation to adverse pregnancy outcome such as birth growth. Sixty-one pregnant women who completed a questionnaire at Ankara Education and Research Hospital, Department of Obstetrics and Gynecology have enrolled in this study. GSTM1 and OGG1 ser326Cys gene polymorphisms were analysed by RFLP-PCR (Restriction Fragment Length Polymorphism-Polymerase Chain Reaction) as possible genetic factors affecting susceptibility to such health effects of smoking and chromosomal damage w... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3244719 Tue, 02 Feb 2010 00:00:00 +0100 3244719 http://www.medworm.com/index.php?rid=3228287&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20119870%26dopt%3DAbstract Authors: Sobti RC, Thakur H, Gupta L, Janmeja AK, Seth A, Singh SK Two genes HPC/ELAC-2 and AAT were studied in north Indian population. HPC/ELAC-2 was studied in prostate cancer cases and AAT was studied in COPD patients. HPC/ELAC-2 is considered as an important cancer-susceptibility gene in prostate cancer. There are two common polymorphisms of this gene, i.e., Ser217Leu and Ala541Thr. Alpha 1 antitrypsin is a highly polymorphic anti-elastase enzyme, especially active in the protection of alveoli and liver. In the present study, we observed the distribution of two deficient alleles PiZ and Pi S in COPD patients. We extracted the DNA from 157 prostate cancer cases, 200 COPD patients, 170 BPH and 370 healthy controls. The polymorphisms were studied by PCR-RFLP technique. The mutant gen... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3228287 Tue, 02 Feb 2010 00:00:00 +0100 3228287 http://www.medworm.com/index.php?rid=3224220&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20111907%26dopt%3DAbstract In this study, we aim to establish the human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM which can be grow and passaged steadily in 1 mug/ml concentration of adriamycin in appropriate medium. The human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM was established using the method of exposure to medium with adriamycin alternated between high and low concentration with gradually increasing concentration. Furthermore, QBC939 and QBC939/ADM were both treated with adriamycin, mitomycin and vindesine, and then detected by MTT assay, respectively. Growth cycle and intra-cellular concentrations of ADM within cells of each group were determined by flow cytometry. Expression levels of P-glycoprotein were detected by Western bolt and real-time PCR. Results showed that,... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3224220 Fri, 29 Jan 2010 00:00:00 +0100 3224220 http://www.medworm.com/index.php?rid=3224219&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20111908%26dopt%3DAbstract Authors: Silva BC, de Vargas Wolfgramm E, da Costa Aguiar VR, Malta FS, de Castro AM, de Souza Ferreira AC, de Paula F, Louro ID Allelic frequencies and other population data analysis are reported for the 15 autosomal Short Tandem Repeats (STR) loci included in the PowerPlex((R))16 kit (CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TPOX, TH01 and vWA) in Pomeranian's descendants from the Espirito Santo State (ES), Brazil, third largest population of Pomeranian's descendants in the world. They chose the mountain region of the state for their preferred geographic location, and they have a very peculiar lifestyle with a selective mating behavior which has maintained their characteristics as a relatively pure subpopulation. Blood samples... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3224219 Fri, 29 Jan 2010 00:00:00 +0100 3224219 http://www.medworm.com/index.php?rid=3224218&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20111909%26dopt%3DAbstract In this study, we show that different alpha-dystrobrevin isoforms are present in promyelocytic leukemia (NB4) cells. Only the biggest alpha-dystrobrevin isoform (DB-alpha), which can be important for its function, was expressed in the membrane fraction of NB4 cells; the other alpha-DB isoforms were found in the hydrophilic cell fractions. Employing the immunoprecipitation and mass spectrometry, we identified novel alpha-DB-interacting proteins involved in cytoskeleton reorganization (actin, tropomyosin, gelsolin, tubulin) and signal transduction process (stathmin, prohibitin, RIBA) during proliferation and differentiation of NB4 cells. Our results suggest that alpha-DB isoforms play a central role in cytoskeleton reorganization via their multiple interactions with actin and actin-associati... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3224218 Fri, 29 Jan 2010 00:00:00 +0100 3224218 http://www.medworm.com/index.php?rid=3224217&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20111910%26dopt%3DAbstract Authors: Chen Z, Ding Z, Ma G, Liu N, Qian Q Cellular cholesterol homeostasis is controlled by the sterol-regulatory element binding transcription factors (SREBFs) that are activated by an SREBF cleavage-activating protein (SCAP). SREBF-2 1784G > C single nucleotide polymorphism (SNP, rs2228314) and SCAP 2386A > G variant (rs12487736) are associated with early onset myocardial infarction (MI) and sudden cardiac death in middle-aged men. We investigated whether these two SNPs are determinants of premature coronary artery disease (CAD) in a Chinese population. We studied 431 consecutive patients, including 197 with coronary stenosis >/=50% or previous MI and 234 controls without documented CAD (males <55 years and females <65 years). All subjects were genotyped for two SNP... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3224217 Fri, 29 Jan 2010 00:00:00 +0100 3224217 http://www.medworm.com/index.php?rid=3220813&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107908%26dopt%3DAbstract Authors: Khurshid R, Saleem M, Akhtar MS, Salim A Granzymes kill cells in a variety of ways. They induce mitochondrial dysfunction through caspase dependent and caspase-independent pathways and destroy DNA and the integrity of the nucleus. For gaining a better understanding of the molecular function of granzyme M and its NK cell specificity, structural characterization of this enzyme by molecular modeling as well as its detailed comparison with other granzymes is presented in this study. The study includes mode of action of granzyme M using cationic binding sites, substrate specificity, post-translational structural modification and its functional relationship and interaction of the enzyme with inhibitor in an attempt to explore how the activity of human granzyme M is controlled under ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3220813 Thu, 28 Jan 2010 00:00:00 +0100 3220813 http://www.medworm.com/index.php?rid=3220812&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107909%26dopt%3DAbstract This study examined the osteogenic differentiation of cultured human periosteal-derived cells grown in a three dimensional collagen-based scaffold. Periosteal explants with the appropriate dimensions were harvested from the mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the cells were divided into two groups and cultured for 28 days. In one group, the cells were cultured in two-dimensional culture dishes with osteogenic inductive medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In the other group, the cells were seeded onto a three-dimensional collagen scaffold and cultured under the same conditions. We examined the bioactivity of alkaline phosphatase (ALP), the RT-PCR... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3220812 Thu, 28 Jan 2010 00:00:00 +0100 3220812 http://www.medworm.com/index.php?rid=3220811&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107910%26dopt%3DAbstract Authors: Qi S, Wang Y, Zhou M, Ge Y, Yan Y, Wang J, Zhang SS, Zhang S Study of retina specific genes would offer insights into retinal diseases and treatment. Based on the information from the gene expression profiles of mouse retinas, we here identified a mitochondria-localized glutamic acid-rich protein (MGARP/OSAP) as one of the highly expressed proteins in retina. Sequence analysis revealed that mouse and rat MGARPs have an extra insertion of four consecutive amino acid repeats at the C-terminus, while other homologues do not. MGARP was demonstrated to be localized to the mitochondria and overexpression of MGARP missing N-terminal region causes severe mitochondrial aggregation, implying an important role of MGARP in maintaining mitochondrial morphology. MGARP is highly expressed in... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3220811 Thu, 28 Jan 2010 00:00:00 +0100 3220811 http://www.medworm.com/index.php?rid=3212927&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099082%26dopt%3DAbstract This article reviews recent findings with regard to the possible role of lubricin in the progression of OA, and further discusses lubricin as a novel potential biotherapeutic approaches for the treatment of OA. PMID: 20099082 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3212927 Sat, 23 Jan 2010 00:00:00 +0100 3212927 http://www.medworm.com/index.php?rid=3157888&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058189%26dopt%3DAbstract In this report, we isolated and characterized a novel Ras small monomeric GTPase Rsr1 gene, designated PmRsr1, from yeast-form Penicillium marneffei. The full-length PmRsr1 cDNA sequence is 1,866 bp in size, and contains an open reading frame of 642 bp encoding 213 amino acids. The predicted molecular mass of PmRsr1 is 24.41 kDa with an estimated theoretical isoelectric point of 9.21. The deduced amino acid sequence of PmRsr1 shows 87% identity with that of Aspergillus fumigatus and A. clavatus. Eight exons and seven introns are identified within the 2,102 bp PmRsr1 genomic DNA sequence of P. marneffei. The open reading frame was subcloned into the pcDNA6-myc-His B expression vector, and the recombinant plasmid was transfected into Vero cell line. The expressed fusion protein was analyzed ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3157888 Fri, 08 Jan 2010 00:00:00 +0100 3157888 http://www.medworm.com/index.php?rid=3130023&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20039139%26dopt%3DAbstract Authors: Xu ZQ, Huang X, Feng C, Tian N, Xu D, Feng SZ Type-II embryonic calli were induced from immature embryos of maize (Zea mays L.) genotype YD and bombarded with beta-glucuronidase gene. Bombarded calli were proliferated on normal N6 medium for 2 weeks at 26 degrees C in the dark and selected on N6 medium containing 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 mg/l phosphinothricin (PPT) but without casamino acids and proline under the same conditions for 14 days. Regeneration was carried out on hormone-free MS medium containing 5 mg/l phosphinothricin at 26 degrees C under 3000 lux illumination. Plants over 8 cm were transplanted into soil and sprayed with 250 mg/l phosphinothricin when two new leaves appeared. Except normal transgenic plants, chimaeric transgenics also w... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3130023 Tue, 29 Dec 2009 00:00:00 +0100 3130023 http://www.medworm.com/index.php?rid=3122593&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20033492%26dopt%3DAbstract Authors: Yang R, Wu X, Yan P, Li X One day at dusk in August, 200X, an airplane was struck by a bird at a Chinese airport (M Airport). After a careful check, some blades of the plane's engine were found to be out of shape and a few feathers and some bloodstains were found in the air intake of the engine. In order to know which species of bird was involved in the birdstrike, firstly we extracted DNA from the bloodstains; secondly, the DNA barcode (portion of COI gene) of the unknown species was amplified by PCR method; thirdly, sequence divergences (K2P differences) of the DNA barcode between the unknown species and a library of 59 common bird species distributed at the airport area were analyzed. Furthermore, a neighbor-joining (NJ) tree based on COI barcodes was created to provide gra... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3122593 Thu, 24 Dec 2009 00:00:00 +0100 3122593 http://www.medworm.com/index.php?rid=3067405&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967452%26dopt%3DAbstract Authors: Wang LN, Yu B, Han GQ, He J, Chen DW Antimicrobial peptides will be attractive and potential candidates as peptide drugs because of their efficient action against microbes and low toxicity to mammal cells. To improve their antibacterial activity, some modifications needs to be made. In this research, the hybrid peptide gene Attacin-Thanatin with 642 bp in length with preferred codons of E. coli was generated using the technology of Gene splicing by overlap extension. The gene was inserted in-frame into E. coli expression plasmid pET-32a (+) and induced to express in E. coli Rosetta. The recombinant protein was partial purified and its biological activity was determined. Analysis of the E. coli Rosetta induced with IPTG revealed that the molecular weight of fusion protein was a... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3067405 Sat, 05 Dec 2009 00:00:00 +0100 3067405 http://www.medworm.com/index.php?rid=3067404&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967453%26dopt%3DAbstract In this study, a cyclin D1 cosmid probe was isolated and used with fluorescence in situ hybridization (FISH) to identify the gene in chromosomal spreads of 12 breast cancer cell lines. Nine cell lines showed increased gene copy levels of cyclin D1, including Five cell lines had more than six copies of cyclin D1 on sister chromatids and four had more than four copies but less than six copies grouped at the chromosome 11 q13 band. Three cell lines had two "normal" chromosome 11 and one and two additional derivative chromosome 11's with three and four 11q13 sites which lacked amplification of cyclin D1 on any of these sites. Using progesterone receptor (PR) gene as an internal control, a 2.0-fold or greater increase in cyclin D1 gene signals, was observed in five of the ten cell lines by Sout... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3067404 Sat, 05 Dec 2009 00:00:00 +0100 3067404 http://www.medworm.com/index.php?rid=3067403&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967454%26dopt%3DAbstract Authors: Lee JH, Kim YJ, Jeong DY, Sathiyaraj G, Pulla RK, Shim JS, In JG, Yang DC Glutamate decarboxylase (GAD) catalyzes the conversion of L: -glutamate to gamma-aminobutyric acid (GABA). A full-length cDNA encoding GAD (designated as PgGAD) was isolated and characterized from the root of Panax ginseng C. A. Meyer. The length cDNA of PgGAD was 1881 bp and contained a 1491 bp open reading frame (ORF) encoding a glutamate decarboxylase protein of 496 amino acids, possessing a Ser-X-X-Lys active site, which belongs to the GAD group. The deduced amino acid sequence of the PgGAD was classified in the plant GAD family and has 76-85% high similarity with other plants as like petunia, Arabidopsis, tomato. Secondary structure of PgGAD was predicted by using SOPMA software program. Southern bl... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3067403 Sat, 05 Dec 2009 00:00:00 +0100 3067403 http://www.medworm.com/index.php?rid=3067402&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967455%26dopt%3DAbstract Authors: Tahernezhad Z, Zamani MJ, Solouki M, Zahravi M, Imamjomeh AA, Jafaraghaei M, Bihamta MR Aegilops tauschii Coss. is a diploid (2n = 2x = 14,DD) goat grass species which has contributed the D genome in common wheat. Genetic variations in 28 accessions of Aegilops tauschii belonged to different provinces of Iran, were evaluated using 16 morphological traits and 19 SSR markers. In number of spikelet per spike and plant height, there was a high variation in ssp. tauschii and ssp. strangulata respectively and for days to mature a low variation in both subspecies was found. Discriminant function analysis showed that 67.9% of original grouped cases correctly classified. Factor analysis indicated that three factor explain 66.49% of total variation. The three clusters revealed by the cl... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3067402 Sat, 05 Dec 2009 00:00:00 +0100 3067402 http://www.medworm.com/index.php?rid=3057265&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19953327%26dopt%3DAbstract In conclusion, our data has identified the first transcription factor involved in P2X receptor expression. PMID: 19953327 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3057265 Wed, 02 Dec 2009 00:00:00 +0100 3057265 http://www.medworm.com/index.php?rid=3049050&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19949872%26dopt%3DAbstract Authors: Zhang M, Xia H, Li X, Wang X, Dong Y, Zhang T, Yu H CalDAGs are a family of Ras guanyl exchange factors that contain calcium and DAG-binding domains. Among the four identified members of CalDAG family, CalDAGIII has been shown to play important role in B lymphocyte and endocrine cell functions. However, the mechanism underlining these functions remain to be determined. Here in the present study, we determined the subcellular localization of CalDAGIII and roles of calcium-binding and DAG-binding domains in its localization. We found that C1 domain but not EF hands is important for both CalDAGIII localization to the Golgi and p38 activation in B cells, indicating that CalDAGIII may be regulated by DAG but not Calcium. PMID: 19949872 [PubMed - as supplied by publisher] (Sourc... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3049050 Tue, 01 Dec 2009 00:00:00 +0100 3049050 http://www.medworm.com/index.php?rid=3049049&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19949873%26dopt%3DAbstract In this study, we used PCR-SSCP to analyse ovine KRTAP1-4 (previously B2D), a gene encoding a member of the KAP1-x family. Nine different PCR-SSCP patterns were detected in the 320 sheep that were analysed. Either one or a combination of two patterns was observed for each sheep, which was consistent with these sheep being either homozygous or heterozygous for this gene. DNA sequencing revealed that these patterns represent nine different DNA sequences. All of these sequences were unique, but shared a high homology with the published ovine KRTAP1-4 sequence, suggesting that these sequences represent allelic variants of KRTAP1-4. There were a total of 14 single nucleotide polymorphisms (SNPs) identified and these SNPs tended to be clustered in two regions. Of the 13 SNPs found in the coding ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3049049 Tue, 01 Dec 2009 00:00:00 +0100 3049049 http://www.medworm.com/index.php?rid=3049048&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19949874%26dopt%3DAbstract Authors: Wang J, Zhang WW, Liu JN, Cao YL, Bai XT, Gong YS, Cen PL, Yang MM An alkaline active xylanase, XynBYG, was purified from an alkaliphilic Bacillus pumilus BYG, which was newly isolated from paper mill effluent. It had an optimum pH of 8.0-9.0, and showed good stability after incubated at pH 9.0 for 120 min. The optimum temperature for the activity was 50 degrees C, and the enzyme retained below 55% of its original activity for 30 min at 55 degrees C. The gene coding for XynBYG consists of 687 bp and encodes 229 amino acids. Similarity analysis indicated that XynBYG belong to family 11 glycosyl hydrolases. Site-directed mutagenesis was performed to replace five sites (Tyr/Ser) to Arg/Glu and the results demonstrated that the optimum temperature of the mutant Y7 (S39R-T146E) inc... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3049048 Tue, 01 Dec 2009 00:00:00 +0100 3049048 http://www.medworm.com/index.php?rid=3049051&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946753%26dopt%3DAbstract Authors: Sathiyaraj G, Srinivasan S, Subramanium S, Kim YJ, Kim YJ, Kwon WS, Yang DC Polygalacturonase inhibiting proteins (PGIPs) are the major defense proteins which play an important role in resistance to infection of pathogens. A putative novel gene encoding PGIP was isolated from Panax ginseng C.A. Meyer, which shows 70.3 and 68.4% homology with chick pea and Arabidopsis PGIPs. The RACE PCR was preformed to isolate the full-length PGIP cDNA from Panax ginseng. Sequence analysis revealed that the cDNA of PgPGIP is of 1,275 bp in length and that it's containing ORF encodes for a polypeptide of 366 amino acids. Domain analysis revealed that the deduced amino acid sequences of PgPGIP have a typical PGIP topology. The transcription level of PgPGIP was up-regulated in ginseng in respons... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3049051 Sat, 28 Nov 2009 00:00:00 +0100 3049051 http://www.medworm.com/index.php?rid=3036448&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943115%26dopt%3DAbstract This study will improve the genetic engineering of ginseng plant and ginsenosides rich plant development. One dimensional data will lead to the two and three dimensional data. PMID: 19943115 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3036448 Fri, 27 Nov 2009 00:00:00 +0100 3036448 http://www.medworm.com/index.php?rid=3036447&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943116%26dopt%3DAbstract Authors: Barzegari A, Hejazi MA, Hosseinzadeh N, Eslami S, Mehdizadeh Aghdam E, Hejazi MS Pharmaceutical recombinant proteins are widely used in human healthcare. At present, several protein expression systems are available to generate therapeutic proteins. These conventional systems have distinct advantages and disadvantages in protein yielding; in terms of ease of manipulation, the time required from gene transformation to protein purification, cost of production and scaling-up capitalization, proper folding and stability of active proteins. Depending on the research goal and priorities, a special system may be selected for protein expression. However, considering the limited variety of organisms currently used and their usage restrictions, there are still much more pharmaceutical pr... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3036447 Fri, 27 Nov 2009 00:00:00 +0100 3036447 http://www.medworm.com/index.php?rid=3036446&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943117%26dopt%3DAbstract Authors: Wang ZW, Li XY, Tang ZL, Yang SL, Ying ZZ, Fu T, Fan B, Mu YL, Ao H, Li K F-box proteins are quite significant ubiquitin-proteasome pathway regulators in eukaryotic cells. FBXO40, a member of this large family, alters its expression pattern in muscle atrophy. Here we isolated most of the verified porcine FBXO40 coding sequence (CDS) (2258 bp) and assigned it to the porcine chromosome 13q4.1-4.6 by using the INRA-Minnesota porcine radiation hybrid panel, and we also explored the tissue expression distributions, which is relatively high in longissimus dorsi muscle, heart, low in kidney, small intestine, brain, hypophysis, lymphonode, thymus, spleen, large intestine, ovary, stomach, and undetectable in testis, liver, uterus and thyroid gland. Inferring phylogenetic tree was const... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3036446 Fri, 27 Nov 2009 00:00:00 +0100 3036446 http://www.medworm.com/index.php?rid=3032432&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19941075%26dopt%3DAbstract Authors: Lee YH, Woo JH, Choi SJ, Ji JD, Song GG The monocyte chemoattractant protein-1 (MCP-1) promoter -2518 A/G polymorphism has been reported inconsistently to be associated with systemic lupus erythematosus (SLE) and lupus nephritis (LN). The aim of this study was to explore whether the MCP-1 polymorphism confers susceptibility to SLE or LN. We surveyed studies on the MCP-1 polymorphism and SLE or LN identified by MEDLINE or manual searches. Meta-analysis was conducted on the AA genotype (recessive effect), AA, and AG genotypes (dominant effect), AA versus GG (genotype contrast), and on the A allele of MCP-1 in SLE and LN in each ethnic population studied and on all subjects. Ten studies, which included 1,739 SLE patients and 1,680 controls, were included in our meta-analysis. The... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3032432 Thu, 26 Nov 2009 00:00:00 +0100 3032432 http://www.medworm.com/index.php?rid=3032431&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19941076%26dopt%3DAbstract Authors: Carpi FM, Vincenzetti S, Micozzi D, Vita A, Napolioni V Cytidine deaminase (CDA) is a pyrimidine salvage pathway enzyme that catalyzes the hydrolytic deamination of cytidine and deoxycytidine to their corresponding uracil nucleosides. CDA also catalyzes the inactivation of some chemotherapeutic nucleoside analogues such as cytosine arabinoside and gemcitabine. CDA 79A > C (K27Q, rs2072671) and 208G > A (A70T, rs60369023) were found to be associated either with clinical outcomes as well as with pharmacokinetics and toxicity of drugs administered to different subsets of patients. In this paper we reported two PCR-based methods for CDA 79A > C (K27Q) and 208G > A (A70T) genotyping and tested their feasibility using DNA extracted from whole blood as well as from buccal... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3032431 Thu, 26 Nov 2009 00:00:00 +0100 3032431 http://www.medworm.com/index.php?rid=3009061&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19921547%26dopt%3DAbstract Authors: Yonggang L The mRNA differential display technique was performed to investigate the differences of gene expression in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs. One novel gene differentially expressed was identified through semi-quantitative RT-PCR and its cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence analysis revealed that the open reading frame of this gene encodes a protein of 384 amino acids has high homology with the mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) of eight species-cattle (96%), horse (96%), rhesus monkey (93%), rabbit (77%), human ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3009061 Wed, 18 Nov 2009 00:00:00 +0100 3009061 http://www.medworm.com/index.php?rid=3000682&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19911306%26dopt%3DAbstract Authors: Liu H, Zhang Y, Li S, Yan Y, Li Y Glutamate decarboxylase produces GABA, the main inhibitory neurotransmitter in adult mammalian brain. Two homologous forms of GAD encoded by separate genes have been identified in mammalian brain, with molecular weight of 67 kDa (GAD67) and 65 kDa (GAD65). Here, we studied the transcriptional regulation of GAD67. Three transcript variants (GAD67A, GAD67B, and GAD67C) transcribed from distinct categories of transcriptional start sites were identified. RT-PCR revealed these transcripts have distinct tissues distributions. Though GAD67A and GAD67B were co-expressed in brain and many nonneural tissues, in heart, only GAD67A was expressed. GAD67C was specifically expressed in testis. These transcripts also showed distinct developmental expression p... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=3000682 Fri, 13 Nov 2009 00:00:00 +0100 3000682 http://www.medworm.com/index.php?rid=2991947&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19908163%26dopt%3DAbstract In this study we demonstrate the presence of an elastase homologue, named BbElas, in Branchiostoma belcheri. The recombinant BbElas hydrolyses the elastase specific substrate N-succinyl-Ala-Ala-Ala p-nitroanilide, which can be inhibited by the serine proteinase inhibitor PMSF, the elastase-specific inhibitor elastatinal and the cysteine proteinase inhibitor PCMB. Phylogenetic analysis shows that BbElas represents the archetype of vertebrate elastases, hinting at the clues that the different isoforms of vertebrate elastases are originated from an ancestral gene like BbElas. Our results also suggest that the mid-gut in amphioxus is to homologous vertebrate pancreas, a novel proposal which deserves further study. PMID: 19908163 [PubMed - as supplied by publisher] (Source: Molecular Biolog... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2991947 Thu, 12 Nov 2009 00:00:00 +0100 2991947 http://www.medworm.com/index.php?rid=2991946&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19908164%26dopt%3DAbstract Authors: Chen LL, Zhu TB, Yin H, Huang J, Wang LS, Cao KJ, Yang ZJ Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) may play an important role in attenuating cardiac remodeling and apoptosis after myocardial infarction. However, the anti-inflammation effects of eNOS in infarcted myocardium and the role of MAPK signaling in eNOS/NO mediated cardiac remodeling have not yet been elucidated. Adenovirus carrying Human eNOS gene was delivered locally into heart 4 days prior to induction of myocardial infarction (MI) by left anterior descending coronary artery ligation. Monocyte/macrophage infiltration was detected by ED-1 immunohistochemistry. Western blot was employed to examine the activation of MAPK. eNOS gene transfer significantly reduced myocardial infarct size and improv... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2991946 Thu, 12 Nov 2009 00:00:00 +0100 2991946 http://www.medworm.com/index.php?rid=2977957&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19898993%26dopt%3DAbstract In this study the role of genetically determined individual NAT1 and NAT2 genotypes, haplotypes and haplotype combinations in the predisposition to head and neck cancer was investigated. Polymorphic regions of the NAT1 and NAT2 genes were analyzed in patients with head and neck cancer and healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. Distribution of the genotypes, allele frequencies, diplotypes and haplotypes and correlation with clinical characteristics were evaluated. No association was observed between the NAT1*3, NAT1*10, NAT1*11, NAT2*5 and NAT2*6 genotypes and risk of head and neck cancer. The NAT2*7 slow genotype was associated with reduced risk of disease. A significant association was observed between the fast acetylator NAT2*4/NAT1*10 ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2977957 Sun, 08 Nov 2009 00:00:00 +0100 2977957 http://www.medworm.com/index.php?rid=2972221&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19894142%26dopt%3DAbstract Authors: Zhao YP, Zhang CM, Zhu C, Chen XH, Wang JL, Ji CB, Chi X, Hong Q, Peng YZ, Guo XR NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects and is involved in insulin resistance. In the present study, the mRNA expression of NYGGF4 homologous genes was examined in the 3T3-L1 cell line. The NYGGF4 mRNAs were expressed at low levels in the 3T3-L1 preadipocytes. During the conversion of 3T3-L1 preadipocytes to adipocytes, the expression of NYGGF4 mRNA was upregulated. On the 8th day after induction of differentiation, the NYGGF4 mRNA levels peaked and remained high. Free fatty acids (FFA) and tumor necrosis factor-alpha (TNFalpha) could upregulate NYGGF4 mRNA expression in 3T3-L1 adipocytes, while interleukin-6 (IL-6), leptin, and resistin exerted... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2972221 Fri, 06 Nov 2009 00:00:00 +0100 2972221 http://www.medworm.com/index.php?rid=2968119&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888673%26dopt%3DAbstract Authors: Zhang D, Ma J, Jiang J, Qiu L, Zhu C, Su T, Li Y, Wu K, Jiang S The lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) plays an important function in the innate immune response of invertebrates as a pattern recognition receptor (PRR). Herein, we described the isolation and characterization of pearl oyster Pinctada fucata LGBP (designated as poLGBP). The poLGBP cDNA was 2,075 bp long and consisted of a 5'-untranslated region (UTR) of 18 bp, a 3'-UTR of 299 bp with one cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 1,758 bp encoding a polypeptide of 585 amino acids with an estimated molecular mass of 65.1 kDa and a theoretical isoelectric point of 5.80. Homology analysis of the deduced amino acid sequence of the poLGBP with other known LGB... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2968119 Wed, 04 Nov 2009 00:00:00 +0100 2968119 http://www.medworm.com/index.php?rid=2968118&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888674%26dopt%3DAbstract Authors: Sun Q, Cai Y, Xie Y, Mo J, Yuan Y, Shi Y, Li S, Jiang H, Pan Z, Gao Y, Chen M, He X To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation System) indicate that these genes are involved in 68 biochemical pathways. These ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2968118 Wed, 04 Nov 2009 00:00:00 +0100 2968118 http://www.medworm.com/index.php?rid=2968117&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888675%26dopt%3DAbstract Authors: Shen X, Martens S, Chen M, Li D, Dong J, Wang T As a key enzyme in the biosynthesis of flavonols, anthocyanidins and proanthocyanidins, flavanone-3ss-hydroxylase (F3H) plays very important roles in plant stress response. A putative flavanone-3ss-hydroxylase gene from Medicago truncatula (MtF3H), a model legume species, was identified from a bio-data analysis platform. It was speculated to be induced by salt stress based on the outcomes of the analysis platform. The complementary DNA (cDNA) consists of 1499 bp with an open reading frame (ORF) of 1098 bp, which encodes a putative protein of 365 amino acids with a molecular weight of about 41.36 kDa and an isoelectric point of 5.60. To measure the catalytic activity of the protein, the MtF3H gene was ligated to pYES2 vector and h... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2968117 Wed, 04 Nov 2009 00:00:00 +0100 2968117 http://www.medworm.com/index.php?rid=2968115&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888676%26dopt%3DAbstract Authors: Xu H, Li K, Yang F, Shi Q, Wang X In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt and osmotic stresses, transgenic tobacco plants constitutively overexpressing CsNMAPK were generated. Northern and Western blot analysis showed that strong signals were detected in the RNA and protein samples extracted from transgenic lines, whereas no signal was detected in the wild type tobacco, indicating that CsNMAPK was successfully transferred into tobacco genome and overexpressed. The... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2968115 Wed, 04 Nov 2009 00:00:00 +0100 2968115 http://www.medworm.com/index.php?rid=2959949&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885739%26dopt%3DAbstract In this study, a novel cotton annexin gene (designated as GhFAnnx) was isolated from a fiber cDNA library of cotton (Gossypium hirsutum). The full-length cDNA of GhFAnnx comprises an open reading frame of 945 bp that encodes a 314-amino acid protein with a calculated molecular mass of 35.7 kDa and an isoelectric point of 6.49. Genomic GhFAnnx sequences from different cotton species, TM-1, Hai7124 and two diploid progenitor cottons, G. herbaceum (A-genome) and G. raimondii (D-genome) showed that at least two copies of the GhFAnnx gene, each with six exons and five introns in the coding region, were identified in the allotetraploid cotton genome. The GhFAnnx gene cloned from the cDNA library in this study was mapped to the chromosome 10 of the A-subgenome of the tetraploid cotton. Sequence a... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2959949 Tue, 03 Nov 2009 00:00:00 +0100 2959949 http://www.medworm.com/index.php?rid=2959948&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885740%26dopt%3DAbstract Authors: Peng J, Lai L, Wang X GASA-like genes form multigene families in diverse plant species and encode the proteins with a unique cysteine-rich domain (GASA domain). In our previously work, we cloned a GASA-like gene PRGL (Proline-rich GASA-like) from gerbera. Here we report the expression profiles of PRGL and the subcellular localization of PRGL protein. Multiple sequence alignment of the GASA domains indicates that PRGL shows the highest homology to AtPRGL (73.3% of amino acid identity) from Arabidopsis. Phylogenic analysis based on the full amino acid sequences indicates that PRGL and AtPRGL belong to a novel subfamily of GASA proteins. Northern blot assay showed that PRGL is highly expressed in young flower, young leaf and young root, whereas hardly detected when these organs b... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2959948 Tue, 03 Nov 2009 00:00:00 +0100 2959948 http://www.medworm.com/index.php?rid=2959947&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885741%26dopt%3DAbstract Authors: Liu Q, Zhang N, Li L, Liu J Elymus L. is the largest genus in Triticeae, containing about 150 species with four recognized genome donors (St, H, P, and W). Traditionally, the genome compound of this genus is identified based on cytological data. Recently, molecular phylogenetic analysis was used to investigate its genomic combination. Here we describe a restriction fragment length polymorphism (RFLP) assay based on digesting alcohol dehydrogenase (Adh) amplicons with two restriction enzyme combinations, EcoRI-HindIII and EcoRI-PstI, which easily can be used to distinguish Elymus and its closely related genera genomes. The method includes only four steps: (1) amplifying nuclear Adh genes with universal primers; (2) purifying and cloning PCR products; (3) digesting plasmids with... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2959947 Tue, 03 Nov 2009 00:00:00 +0100 2959947 http://www.medworm.com/index.php?rid=2959946&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885742%26dopt%3DAbstract Authors: Yuan FL, Chen FH, Lu WG, Li X Acid-sensing ion channels 3 (ASIC3) is the most sensitive to such a pH change, predominantly distributed in the sensory peripheral nervous system, and strongly correlated with pain. Recently, there is increasing evidence that ASIC3 may contribute to the pathogenesis of chronic inflammatory pain diseases due to it is predominantly expressed in dorsal root ganglia (DRG) neurons making it a good candidate for a pain sensor. Elevated expression of ASIC3 was found in DRG of rodents with inflamed hind paws. In addition, it has been shown that ASIC3 gene knock-out mice (ASIC3-/-) exhibited no enhanced hyperalgesia in inflamed joint. All theses findings suggest that ASIC3 have important biological effects in inflammation that might be a promising therapeu... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2959946 Tue, 03 Nov 2009 00:00:00 +0100 2959946 http://www.medworm.com/index.php?rid=2951518&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19882361%26dopt%3DAbstract Authors: Liao RY, Mao C, Qiu LX, Ding H, Chen Q, Pan HF Published data on the association between TGFBR1*6A/9A polymorphism and cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. A total of 32 studies including 13,662 cases and 14,147 controls were involved in this meta-analysis. Overall, significantly elevated cancer risks were associated with TGFBR1*6A in all genetic models (for allelic effect: OR = 1.11; 95% CI = 1.03-1.21; for 6A/6A vs. 9A/9A: OR = 1.30; 95% CI = 1.01-1.69; for 9A/6A vs. 9A/9A: OR = 1.08; 95% CI = 1.01-1.15; for dominant model: OR = 1.08; 95% CI = 1.02-1.15; for recessive model: OR = 1.29; 95% CI = 1.00-1.68). In the subgroup analysis by cancer types, significant associations were found in breast ca... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2951518 Sun, 01 Nov 2009 00:00:00 +0100 2951518 http://www.medworm.com/index.php?rid=2951519&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19882234%26dopt%3DAbstract Authors: Yang W, Liu X, Zhang J, Feng J, Li C, Chen J Potato (Solanum tuberosum) is an important crop around the world, and accounts for a significant amount of the food consumed by humans. However, little information is available about potato miRNAs which play important regulatory roles in plant growth and development. In the present study, computational prediction of potential miRNAs from potato revealed 71 miRNAs belonging to 48 families. Amongst these 71 mRNAs, 65 were predicted for the first time. Most potato miRNA families have one to three members, and sequence analysis showed that the candidate pre-miRNA sequences varied from 48 to 224 bp in length. To verify the predicted miRNAs, specific stem-loop RT primers were designed and real-time PCR assays were used to profile the expr... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2951519 Sat, 31 Oct 2009 00:00:00 +0100 2951519 http://www.medworm.com/index.php?rid=2948446&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19876763%26dopt%3DAbstract Authors: Zhang GY, Wu J, Wang XW Rapid alkalinization factors (RALFs) are recently reported active peptide hormones and are considered to play important roles in plant development. We previously identified a differentially expressed cDNA fragment between cabbage flower buds of sterility lines and its maintainer line, which showed significant homology to Arabidopsis RALFL9. The novel RALF cDNA (BoRALF1) was isolated from broccoli flower buds by EST assembly. The open reading frame (ORF) comprises 240 bp, encoding a small putative preprotein of 79 amino acids (molecular weight of 8.72 kDa and a pI of 7.8), which contains the mature polypeptide at its C terminus. BoRALF1 shares 70.3% identity with Arabidopsis RALFL9, but has only moderate similarity with functionally characterized RALFs (... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2948446 Fri, 30 Oct 2009 00:00:00 +0100 2948446 http://www.medworm.com/index.php?rid=2948445&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19876764%26dopt%3DAbstract Authors: Bao JP, Chen WP, Feng J, Hu PF, Shi ZL, Wu LD Leptin has been shown to play a crucial role in the regulation of body weight. There is also evidence that this adipokine plays a key role in the process of osteoarthritis. However, the precise role of leptin on articular cartilage metabolism is not clear. We investigate the role of leptin on articular cartilage in vivo in this study. Recombinant rat leptin (100 mug) was injected into the knee joints of rats, 48 h later, messenger RNA (mRNA) expression and protein levels of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), matrix metalloproteinases 2 and 9 (MMP-2, MMP-9), cathepsin D, and collagen II from articular cartilage were analyzed by real-time quantitative polymerase chain reaction (PCR) and ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2948445 Fri, 30 Oct 2009 00:00:00 +0100 2948445 http://www.medworm.com/index.php?rid=2948444&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19876765%26dopt%3DAbstract Authors: He X, Zhang Y, Yu Z Rieske protein gene in the Pacific oyster Crassostrea gigas was obtained by in silico cloning for the first time, and its expression profiles and subcellular localization were determined, respectively. The full-length cDNA of Cgisp is 985 bp in length and contains a 5'- and 3'-untranslated regions of 35 and 161 bp, respectively, with an open reading frame of 786 bp encoding a protein of 262 amino acids. The predicted molecular weight of 30 kDa of Cgisp protein was verified by prokaryotic expression. Conserved Rieske [2Fe-2S] cluster binding sites and highly matched-pair tertiary structure with 3CWB_E (Gallus gallus) were revealed by homologous analysis and molecular modeling. Eleven putative SNP sites and two conserved hexapeptide sequences, box I (THLGC) a... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2948444 Fri, 30 Oct 2009 00:00:00 +0100 2948444 http://www.medworm.com/index.php?rid=2948443&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19876766%26dopt%3DAbstract Authors: Wang W, Jin B, Li W, Xu CX, Cui FA, Liu B, Yan YF, Liu XX, Wang XL The expression of Ornithine decarboxylase (ODC) which is the first key enzyme of polyamine biosynthesis is increased in cancer cells. We had blocked the polyamine synthesis pathway using the adenoviral-mediated antisense ODC in some cancer cells such as prostate cancers and colorectal cancers. These researches demonstrated that ODC antisense expression could inhibit tumor cell growth. In order to reach the goal of applying the targeting gene therapy in clinical practice, we cloned the antisense ODC RNA which was driven by cancer specific promoter (hTERT promoter; telomerase reverse transcriptase promoter) into the adenovirus vector (rAd-CMV-GFP-hTERTp-ODC). Human cancer cell lines (HepG2, Bel-7402, A549) and no... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2948443 Fri, 30 Oct 2009 00:00:00 +0100 2948443 http://www.medworm.com/index.php?rid=2924060&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19851886%26dopt%3DAbstract Authors: Yang L, Liu N, Hu X, Zhang W, Wang T, Li H, Zhang B, Xiang S, Zhou J, Zhang J TNFAIP1 is a protein which can be induced by tumor necrosis factoralpha (TNFalpha) and interleukin-6 (IL-6), it may play roles in DNA synthesis, DNA repair, cell apoptosis and human diseases. However, very little has been known about how TNFAIP1 acts in these physiological processes. In this paper, CK2beta was identified as a partner of TNFAIP1 by screening the HeLa cDNA library in yeast two-hybrid system with TNFAIP1 as a bait. Furthermore, it was demonstrated that CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. It is suggested that the phosphorylation of TNFAIP1 may be required for its functions. ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2924060 Fri, 23 Oct 2009 00:00:00 +0100 2924060 http://www.medworm.com/index.php?rid=2917094&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19847669%26dopt%3DAbstract Authors: Huang P, Zhang Y, Jiang T, Zeng W, Zhang N Glomerulosclerosis is considered to be the final pathway leading to the progressive loss of renal function in several kidney diseases, transforming growth factor beta1 (TGF-beta1) plays a critical role in glomerulosclerosis. However, the mechanisms of TGF-beta1 stimulating glomerulosclerosis remain poorly understood. Here we report that TGF-beta1-induced expression of fibronectin (FN) depends on the activity of aldose reductase (AR) in human mesangial cells (HMCs).The results show that TGF-beta1 increased the expression of FN, which attenuated by pharmacological inhibition of AR or knockdown of the enzyme by small interfering RNA (siRNA). MAPKs (ERK, JNK and p38) signalling pathways were activated in HMCs after stimulated by TGF-beta1... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2917094 Wed, 21 Oct 2009 23:00:00 +0100 2917094 http://www.medworm.com/index.php?rid=2910988&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842061%26dopt%3DAbstract This study contributes to the scarce available information on T. gondii at South America, and reinforces an emerging concept suggesting that SAG2 type I, rather than II, parasites are a frequent cause of clinical toxoplasmosis in this continent. PMID: 19842061 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2910988 Mon, 19 Oct 2009 23:00:00 +0100 2910988 http://www.medworm.com/index.php?rid=2910987&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842062%26dopt%3DAbstract Authors: Wang Y, Sanai K, Wen H, Zhao T, Nakagaki M The silks of both Lepidoptera and its sister order Trichoptera contain a homologue of heavy chain (H-fibroin), which is assumed to determine the physical properties of the fiber, such as elasticity and toughness. The long repetitive region of the H-fibroin caddisfly Stenopsyche marmorata shows a conspicuous hierarchical structure that is composed of huge units, which are mainly constructed from four large blocks (SA, SB, SC and SD) arranged in an orderly fashion. Each block contains short, distinct motifs such as SXSXSX(SX), GPXG(X)(1-3) or triplet GGX, which also occur in lepidopteran and spider filaments. The SA, SB and SC blocks have nearly fixed amino acid numbers, while the length of the SD block varies, usually due to a variable... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2910987 Mon, 19 Oct 2009 23:00:00 +0100 2910987 http://www.medworm.com/index.php?rid=2911012&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842058%26dopt%3DAbstract This study was designed to use comparative proteomics technology to find the differentially expressed proteins between human colorectal carcinoma and the corresponding normal tumor-adjacent colorectal tissues. We have used the highly sensitive two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the identification of proteins differentially expressed in tumoral and neighboring normal mucosa. We have detected differences in abundance of 42 proteins with statistical variance of the tumor versus normal spot volume ratio within the 95th confidence level (Student's t-test; P < 0.05). 10 out of 42 analyzed proteins were unambiguously identified by MS coupled with database interrogation as being ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2911012 Thu, 15 Oct 2009 23:00:00 +0100 2911012 http://www.medworm.com/index.php?rid=2911011&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842059%26dopt%3DAbstract In this study, we expressed two putative mosquito glutamate decarboxylase-like enzymes of mosquitoes and critically analyzed their substrate specificity and biochemical properties. Our results provide clear biochemical evidence establishing that one of them is an aspartate decarboxylase and the other is a glutamate decarboxylase. The mosquito aspartate decarboxylase functions exclusively on the production of beta-alanine with no activity with glutamate. Likewise the mosquito glutamate decarboxylase is highly specific to glutamate with essentially no activity with aspartate. Although insect aspartate decarboxylase shares high sequence identity with glutamate decarboxylase, we are able to closely predict aspartate decarboxylase from glutamate decarboxylase based on the difference of their ac... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2911011 Thu, 15 Oct 2009 23:00:00 +0100 2911011 http://www.medworm.com/index.php?rid=2910998&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842060%26dopt%3DAbstract In this study, we attempted to identify the underlying mechanism. We found that overexpression of p53 protein reduces the level of HBx protein and shortens its half-life, however, in MDM2 knock out cells, p53 has no effects on degradation of HBx, meanwhile, overexpression of MDM2 in absence of p53 can accelerate turnover of HBx protein. These indicate that p53-mediated HBx degradation is MDM2-dependent. MDM2 interacts with HBx in vitro and in vivo but does not promote its ubiquitination. In consistent with the results above, HCC tissue samples with wild-type p53 hardly detect HBx protein, whereas, HBx always accumulate in the tissues with mutant p53. Our data provide a possible mechanism on how p53 regulate HBx stability and also a new clue for the study of p53 mutation and HCC development... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2910998 Thu, 15 Oct 2009 23:00:00 +0100 2910998 http://www.medworm.com/index.php?rid=2902070&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830590%26dopt%3DAbstract Authors: Qiu H, Xu X, Fan B, Rothschild MF, Martin Y, Liu B Porcine MYOD1 gene has been mapped to swine chromosome (SSC) 2p14-p17, which is involved in the regulation of the proliferation and differentiation of skeletal muscle cells. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1) genes, which map close to MYOD1, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. However, little is known about the porcine LDHA and COPB1 genes. In the present study, the full-length cDNA of these two genes were cloned. The mapping results demonstrated that porcine LDHA and COPB1 were all mapped to SSC 2p14-p17. In this region, there are several QTL for growth and carcass traits, including ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2902070 Thu, 15 Oct 2009 23:00:00 +0100 2902070 http://www.medworm.com/index.php?rid=2902068&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19834821%26dopt%3DAbstract Authors: Zhang CM, Gu Y, Qing DN, Zhu JG, Zhu C, Zhang M, Guo XR Uncoupling proteins (UCPs) are a proton transporter family located in the mitochondrial inner membrane. Thus far, five molecules (UCP1-UCP5) have been identified as members of the UCP family. Recently, UCPs have attracted considerable interest in research on energy metabolism and obesity. However, to date, no study has focused on a comprehensive and systematic evaluation of the tissue-specific distribution of UCPs in obese individuals. Our study presents evidence of differential tissue expression profiles of five isoforms of UCPs in normal and diet-induced obese (DIO) rats using real-time polymerase chain reaction (PCR) analysis. The results clearly show that the tissue-specific expression patterns of individual isoforms ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2902068 Thu, 15 Oct 2009 23:00:00 +0100 2902068 http://www.medworm.com/index.php?rid=2902071&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830589%26dopt%3DAbstract In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana x P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2902071 Wed, 14 Oct 2009 23:00:00 +0100 2902071 http://www.medworm.com/index.php?rid=2902069&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830591%26dopt%3DAbstract In this study, we report a functional analysis of SmtA in Arabidopsis thaliana and its response to zinc stress. After high zinc stress, the transgenic plants over-expressing SmtA showed higher survival rate than the wild type. We also found that over-expression of SmtA in Arabidopsis increased the activities of SOD and POD, and enhanced the tolerance to zinc stress. Together, our results indicate that SmtA may play an important role in the response to zinc stress in Arabidopsis. PMID: 19830591 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports) Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2902069 Wed, 14 Oct 2009 23:00:00 +0100 2902069 http://www.medworm.com/index.php?rid=2895318&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826913%26dopt%3DAbstract The objective of this study was to exam the curcumin cytotoxic effect and modulation of two major rate-limiting translation initiation factors, including eIF2alpha and eIF4E protein expression levels in lung adenocarcinoma epithelial cell line A549. Cytotoxicity was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and protein changes were determined by Western blot. A549 cells were treated with 0-240 muM curcumin for 4-96 h. The inhibitory effects of curcumin on cytotoxicity were dose- and time-dependent (P < 0.001). The 50% inhibitory curcumin concentrations (IC50s) at 24, 48, 72, and 96 h were 93, 65, 40, and 24 muM, respectively. Protein expressions of eIF2alpha, eIF4E, Phospho-4E-BP1 were down-regulated, while Phospho-eIF2alpha and Phospho-eIF4E w... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895318 Wed, 14 Oct 2009 23:00:00 +0100 2895318 http://www.medworm.com/index.php?rid=2895323&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826908%26dopt%3DAbstract Authors: Zhou RM, Huang YX, Li XL, Chen C, Shi Q, Wang GR, Tian C, Wang ZY, Jing YY, Gao C, Dong XP Microtubule dynamics is essential for many vital cellular processes such as in intracellular transport, metabolism, and cell division. Evidences demonstrate that alpha-synuclein may associate with microtubular cytoskeleton and its major component, tubulin. In the present study, the molecular interaction between alpha-synuclein and tubulin was confirmed by GST pull-down assay and co-immunoprecipitation. The interacting regions within alpha-synuclein with tubulin were mapped at the residues 60-100 of alpha-synuclein that is critical for the binding activity with tubulin. Microtubule assembly assays and sedimentation tests demonstrated that alpha-synuclein influenced the polymerization of t... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895323 Tue, 13 Oct 2009 23:00:00 +0100 2895323 http://www.medworm.com/index.php?rid=2895322&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826909%26dopt%3DAbstract Authors: Das S, Pan D, Bera AK, Rana T, Bandyopadhyay S, De S, Das SK, Bhattacharya D, Bandyopadhyay SK This communication reports about heat shock protein response after arsenic exposure in broiler chickens in vivo and in vitro both. Splenocytes harvested in presence of sodium arsenite expressed Heat shock protein 70 (HSP 70) which could be identified on the basis of relative migration pattern and western blot analysis. Serum levels of HSP 70 in broiler chicken also increased after continuous feeding of sodium arsenite in drinking water. This particular observation may be attributed towards systematic inflammation, oxidative stress and hepatocellular injury. In vitro relative quantification of transcription level of HSP 70 revealed that splenocytes harvested in presence of sodium arse... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895322 Tue, 13 Oct 2009 23:00:00 +0100 2895322 http://www.medworm.com/index.php?rid=2895321&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826910%26dopt%3DAbstract Authors: Yue T, Fang Q, Yin J, Li D, Li W Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation of porcine muscle satellite cells. Cells isolated from neonatal pig muscle were treated with different concentrations of SAMe (0, 0.5 and 1.0 mM) for 24 h, induced for a 9-day adipogenic differentiation and were finally stained by oil red O staining. The adipocyte determination and differentiation factor-1 (ADD1) and peroxisome proliferator-activated receptor gamma (PPARgamma) mRNA and protein were stimulated by SAMe treatment in ... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895321 Tue, 13 Oct 2009 23:00:00 +0100 2895321 http://www.medworm.com/index.php?rid=2895320&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826911%26dopt%3DAbstract Authors: Peng S, Zhang Y, Zhang J, Wang H, Ren B To study the effects of ketamine on ERK expression in hippocampal neural cell and the ability of learning behavior in minor rats. Seventy-two Sprague-Dawley rats of 21 days old were randomly divided into nine groups. The Y-maze was used to test the ability of learning and spatial localization. At the end of training, all rats were killed and the expression levels of ERK1, ERK2 and p-ERK1/2 were tested by immunohistochemistry. The learning times and total reaction time (TRT) of group K2a, K2b, K2c and K3 have significant differences compared with T group (P < 0.05). Immunohistochemical staining showed that the level of ERK1, ERK2 and p-ERK1/2 in all rats which received light-electricity integrated training increased remarkably relative... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895320 Tue, 13 Oct 2009 23:00:00 +0100 2895320 http://www.medworm.com/index.php?rid=2895319&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826912%26dopt%3DAbstract Authors: Warchoł T, Lianeri M, Lącki JK, Jagodziński PP It has been reported that stromal cell-derived factor-1 (SDF1), currently also designated CXCL12, plays a significant role in the development of nephritis and death in the lupus mice model. Using restriction length fragment polymorphism (RFLP) analysis we assessed the frequencies of SDF1-3' G801A (rs 1801157) polymorphic variants between systemic lupus erythematosus (SLE) patients (n = 150) and controls (n = 300). There were no significant differences in the prevalence of SDF1-3' G801A polymorphic variants in SLE patients and healthy individuals. However, we observed that the SDF1-3' A/A and G/A genotypes (recessive model) contributed to renal manifestations of SLE OR = 3.042 (95% CI = 1.527-6.058, P = 0.002), an... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895319 Tue, 13 Oct 2009 23:00:00 +0100 2895319 http://www.medworm.com/index.php?rid=2895317&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826914%26dopt%3DAbstract In this study we show that PgDREB2A from Pennisetum glaucum is a powerful transcription factor to engineer multiple stress tolerance in tobacco plants. The PgDREB2A protein lacks any potential PEST sequence, which is known to act as a signal peptide for protein degradation. Therefore, the transgenic tobacco plants were raised using full-length cDNA without modification. The transgenics exhibited enhanced tolerance to both hyperionic and hyperosmotic stresses. At lower concentration of NaCl and mannitol, seed germination and seedling growth was similar in WT and transgenic, however at higher concentration germination in WT decreased significantly. D15 and D46 lines showed 4-fold higher germination percent at 200 mM NaCl. At 400 mM mannitol seed germination in WT was completely arrested, whe... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895317 Tue, 13 Oct 2009 23:00:00 +0100 2895317 http://www.medworm.com/index.php?rid=2895316&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826915%26dopt%3DAbstract Authors: Xu HG, Ren W, Lu C, Zhou GP Interferon regulatory factor 3 (IRF-3), an essential transcriptional regulator of the interferon genes, has been implicated in virus and double-stranded RNA mediated induction of IFN-alpha, IFN-beta and RANTES, in virus-induced apoptosis and in tumor suppression. Promoter plays an important role in the regulation of gene expression, but the characterization of the human IRF-3 promoter has not been systematically analyzed in HEK 293 cells. To characterize the human IRF-3 promoter, we have isolated a genomic clone of the human IRF-3 gene promoter region containing 1,000 nucleotides of the 5'- flanking region. Transient transfection of 5'-deleted promoter-reporter constructs and luciferase assay illustrated the region -149/-93 relative to the transcrip... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895316 Tue, 13 Oct 2009 23:00:00 +0100 2895316 http://www.medworm.com/index.php?rid=2895336&cid=s_37699_67_f&fid=37699&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19823944%26dopt%3DAbstract In this study, the results of real time RT-PCR showed that the transcripts of DCA1 were induced by gradient concentration of sodium chloride. Subsequently, a structurally novel promoter containing highly repeated GT/AC sequences of the DCA1 gene was isolated, which was able to drive a stable expression of the foreign bar gene in transformed cells of D. salina, and the gradient concentrations of sodium chloride in media paralleled regulations in the levels of both proteins and mRNA of the bar gene driven by the DCA1 promoter. Furthermore, analysis of GUS activities revealed that the salt-inducible expression of the external gus gene was regulated by the promoter fragments containing highly repeated GT sequences, but not by the promoter fragments deleting highly repeated GT sequences. The fi... Molecular Biology Reports journals http://www.medworm.com/rss/comments.php?id=2895336 Mon, 12 Oct 2009 23:00:00 +0100 2895336