MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Neoplasma' source. Wed, 08 Feb 2012 08:41:51 +0100 http://www.medworm.com/index.php?rid=5660678&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296495%26dopt%3DAbstract Authors: Wieczorek E, Reszka E, Gromadzinska J, Wasowicz W Abstract The family of human matrix metalloproteinases (MMPs) consists of 24 zinc- and calcium-dependent proteolytic enzymes. MMPs are divided into six subgroups, in terms of differences in the substrate specificity with structural domain architecture. These enzymes are involved in many physiological processes, such as skeletal development, wound healing, scar formation, as well as carcinogenesis. MMPs, fulfilling its function of degradation of extracellular matrix components, are involved in one of the stages of angiogenesis enabling the development, growth and spread of the primary tumor. Therefore, the search for the common polymorphic variants of MMPs, new genetic markers as prognostic factors in breast cancer progress ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660678 Thu, 02 Feb 2012 05:00:00 +0100 5660678 http://www.medworm.com/index.php?rid=5660677&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296496%26dopt%3DAbstract Authors: Krejci M, Kamelander J, Pospisil Z, Mayer J Abstract Graft-versus-host disease (GVHD) is the most frequent complication after allogeneic hematopoietic cell transplantation. We analyzed the kinetics of bilirubin and liver enzymes in 47 cases with liver GVHD and in 47 cases without GVHD after allogeneic transplantation for various hematological malignancies. The duration of an liver GVHD episode (LGVHD) was defined as the interval from the point when the criteria of LGVHD were met to the decrease to < 2 upper normal limit (UNL) for aminotransferases or bilirubin < 34 mmol/l for bilirubin. The imminent LGVHD episode was defined as the interval from the start of continuous increase (≥ 3 consecutive rising values) of bilirubin and liver enzymes above UNL to the point of... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660677 Thu, 02 Feb 2012 05:00:00 +0100 5660677 http://www.medworm.com/index.php?rid=5660676&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296497%26dopt%3DAbstract In this study, the human prostatic epithelial cell line RWPE-1 was cultured under three-dimensional (3-D) culture conditions, and the effect of ionizing radiation on acinar morphogenesis and its association with autophagy were discussed. The results illustrated that Formation of specific spheroid (acinar) structures was detectable under 3-D culture conditions. Radiation induced the disruption of acini in different cell models using either gene overexpression (Akt) or gene knock-down (Beclin 1 and ATG7). Introduction of Akt not only accelerated the growth of cells (i.e., caused the cells to manifest elongating and microspike-like structures that are obviously different from structures seen in wild-type RWPE-1 cells under two-dimensional conditions), but also changed their morphological char... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660676 Thu, 02 Feb 2012 05:00:00 +0100 5660676 http://www.medworm.com/index.php?rid=5660675&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296498%26dopt%3DAbstract Authors: Ding R, Chen DJ, Lin SL Abstract Results from published studies on the association of Human Oxoguanine Glycosylase 1 (hOGG1) S326C genetic polymorphism with the risk of gastric cancer are inconsistent. We performed a meta-analysis to summarize the possible association. Eleven case-control studies including 2168 cases and 4058 controls were identified from electronic databases (Pubmed, Elsevier Science Direct, Chinese National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), and the Chinese database, Wanfang). No significant association between hOGG1 S326C genetic polymorphism and risk of gastric cancer was observed in the overall analysis. In the stratified analysis based on ethnicity, still no significant association was observed in Europea... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660675 Thu, 02 Feb 2012 05:00:00 +0100 5660675 http://www.medworm.com/index.php?rid=5660674&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296499%26dopt%3DAbstract In this study, we used human hepatocellular carcinoma cell line SMMC7721 as a model to demonstrate that Stat5 was highly expressed in these cells. Next we showed that RNAi mediated Stat5 knockdown could inhibit the proliferation and induce the apoptosis of SMMC7721 cells in vitro. Furthermore, we demonstrated that Stat5 knockdown inhibited the growth and induced the apoptosis of SMMC7721 cells in xenografts in nude mice. Taken together, our in vitro and in vivo data suggest that Stat5 plays an important role in human hepatocellular carcinoma. Inhibition of Stat5 by RNAi holds promise to be a novel gene therapy vector for hepatocellular carcinoma. Keywords: hepatocellular carcinoma; SMMC7721; RNA interference; Stat5; apoptosis. PMID: 22296499 [PubMed - as supplied by publisher] (Sou... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660674 Thu, 02 Feb 2012 05:00:00 +0100 5660674 http://www.medworm.com/index.php?rid=5660673&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296500%26dopt%3DAbstract The objective of the studies was to explore if the stem cell biomarkers could be used to predict the tumor chemotherapy-resistance in serous ovarian cancer patients. Expression of two putative stem cell markers CD133 and nestin, and vascular epithelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) were detected in 123 cases of advanced serous ovarian cancer specimens by immunohistochemistry. To estimate intra-tumoral microvessel density (MVD), CD34 immunostaining was also performed. CD133 and nestin were defined to be positive in 35.0% and 32.5% of the serous ovarian carcinoma tissues, respectively. It was observed that overexpression of nestin but not CD133 was associated with the cisplatin-based chemotherapy resistance and shorter overall survival of the patients, and ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660673 Thu, 02 Feb 2012 05:00:00 +0100 5660673 http://www.medworm.com/index.php?rid=5660672&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296501%26dopt%3DAbstract Incidence of cutaneous malignant melanoma in the Czech Republic: The risks of sun exposure for adolescents. Neoplasma. 2012 Feb 2;:1-2 Authors: Vranova J, Arenbergerova M, Arenberger P, Stanek J, Vrana A, Zivcak J, Rosina J Abstract The Czech Republic reported one of the highest incidence rate in cutaneous melanoma (CM) in Europe and because this incidence has been increasing, mainly among young people, the main goal of our study was to establish sun exposure behavior risk factors for CM formation and to evaluate whether the young generation of Czechs is exposed to a higher risk of CM than the older generation. A questionnaire-based case-control study was conducted. We obtained 978 completed questionnaires: 216 from patients with CM and 762 from healthy respondents. The... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660672 Thu, 02 Feb 2012 05:00:00 +0100 5660672 http://www.medworm.com/index.php?rid=5660671&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296502%26dopt%3DAbstract In this study, we aimed to investigate whether restoration of sFRP1 affected HCC metastatic behavior.  sFRP1 mRNA expression and promoter methylation in HCC tissues and cell lines were examined using RT-PCR and methylation-specific PCR (MS-PCR), respectively. sFRP1 protein expression was assessed by Western Blot. We generated stable HCC cell line restoration of sFRP1 in HepG2 cells, which naturally do not express detectable sFRP1 mRNA. The effects of exogenous sFRP1 on HepG2 cell invasion were investigated using trans-well assay. Also the effects of sFRP1 re-expression on the β-catenin/T-cell factor-dependent transcription activity was measured by luciferase assay.sFRP1 promoter methylation was frequently observed in HCC tissues (60%) and cell lines (75%). All samples with sFRP1 methylat... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660671 Thu, 02 Feb 2012 05:00:00 +0100 5660671 http://www.medworm.com/index.php?rid=5660670&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296503%26dopt%3DAbstract Authors: Sager O, Beyzadeoglu M, Dincoglan F, Oysul K, Kahya YE, Gamsiz H, Uysal B, Demiral S, Dirican B, Surenkok S Abstract The purpose of this study is to evaluate the effect of Active Breathing Control-moderate deep inspiration breath-hold (ABC-mDIBH) on tumor motion and critical organ doses in non-small cell lung cancer (NSCLC) radiotherapy. 23 patients with locally advanced NSCLC were included in the study. All patients were scanned at free breathing and ABC-mDIBH for radiation treatment planning. 3 separate treatment plans were generated for each patient including one plan with ABC-mDIBH and uniform margins, one plan with free breathing and uniform margins, and one plan with free breathing and 3-dimensional non-uniform margins determined by Cone Beam Computed Tomography (CBC... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660670 Thu, 02 Feb 2012 05:00:00 +0100 5660670 http://www.medworm.com/index.php?rid=5660669&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296504%26dopt%3DAbstract In this report, the promoter region of the APRIL gene was determined and the major transcription factor was investigated for the first time. Deletion analysis of 5'-flanking region of the human APRIL gene and transient transfection revealed that a 538 bp region (from -1539 to -1001) was essential for promoter activation of the APRIL gene. The data from electrophoretic mobility shift assays (EMSA) indicated that the 538 bp promoter region was responsive to the specificity protein 1 (Sp1) and nuclear factor kappa B (NF-kB). Overexpression of Sp1 or NF-kB increased the activity of the APRIL promoter. Mithramycin A (inhibitor of Sp1) and Bay11-7082 (inhibitor of NF-kB) exhibited an inhibitory activity to APRIL promoter. Our results will benefit to the APRIL gene regulation investigation... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5660669 Thu, 02 Feb 2012 05:00:00 +0100 5660669 http://www.medworm.com/index.php?rid=5604980&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248268%26dopt%3DAbstract Time trends in cervical cancer epidemiology in the Slovak Republic: reflection on the non-implementation of screening with international comparisons. Neoplasma. 2012;59(2):121-8 Authors: Ondrusova M, Zubor P, Ondrus D Abstract Cervical cancer is a serious public health problem with high geographical variations in incidence, mainly due to historical patterns of risk factors and the influence of screening activities. To reduce both cervical cancer incidence and mortality is the primary objective of organized screening and annual reports of high quality utilising accepted interventional measures. Currently, the time-trends in overall incidence and mortality from cervical cancer in the Slovak Republic are implacable. The high incidence values of this condition that have stabi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604980 Thu, 19 Jan 2012 04:30:02 +0100 5604980 http://www.medworm.com/index.php?rid=5604979&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248269%26dopt%3DAbstract The objective of this study was to evaluate the feasibility, toxicity and efficacy of postoperative radiochemotherapy with weekly cisplatin in locoregionally advanced or high risk head and neck cancer in a single institutional setting.Patients with head and neck cancer of stage III/IV or patients with insufficient margins of resection were included in the study. Radiotherapy consisted of 70 Gy/ 7 weeks/ 35 fraction after R1/2 resection and 60-64 Gy/ 6-6,5 weeks/ 30-32 fraction after R0 resection, respectively. All patients received concurrent cisplatin 40 mg/m2 weekly.Between 7/2002 and 12/2008, 100 consecutive patients [WHO ≤ 2, male to female ratio 84/16, median age 54 years] were treated. Tumors of the oropharynx were the most frequent (49%) and stage IV was predominant (86%). 96%... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604979 Thu, 19 Jan 2012 04:30:02 +0100 5604979 http://www.medworm.com/index.php?rid=5604978&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248270%26dopt%3DAbstract In conclusion, our results showed high prevalence of activation of mTOR pathway in ICC tumors, suggesting that a high proportion of ICC patients might benefit from mTOR pathway targeted therapies. In addition, p-4EBP1 phosphorylation at Thr 70 could be a useful prognostic biomarker for ICC patients. Keywords: intrahepatic cholangiocarcinoma, mTOR pathway, phosphorylation, targeted therapy, prognosis. PMID: 22248270 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604978 Thu, 19 Jan 2012 04:30:02 +0100 5604978 http://www.medworm.com/index.php?rid=5604977&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248271%26dopt%3DAbstract In conclusion, the major role of DCP is the moderate confirmation of HCC. More prospective studies of DCP are needed in future. Keywords: gastric cancer, proton pump inhibitors, HIF-1α PMID: 22248271 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604977 Thu, 19 Jan 2012 04:30:02 +0100 5604977 http://www.medworm.com/index.php?rid=5604976&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248272%26dopt%3DAbstract In conclusion, the major role of DCP is the moderate confirmation of HCC. More prospective studies of DCP are needed in future. Keywords: hepatocellular carcinoma, des-γ-carboxy prothrombin, pooled analysis, sandwich ELISA, diagnostic test. PMID: 22248272 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604976 Thu, 19 Jan 2012 04:30:02 +0100 5604976 http://www.medworm.com/index.php?rid=5604975&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248273%26dopt%3DAbstract Authors: Dzian A, Halasova E, Matakova T, Kavcova E, Smolar M, Dobrota D, Hamzik J, Mistuna D Abstract Slovak Republic belongs to the countries with high incidence of lung cancer. Gene polymorphisms of the glutathione S-transferases (GSTs) may play a role in individual lung cancer susceptibility. In presented case-control study we investigate the incidence of polymorphism of GSTT1, GSTM1, GSTP1 genes and their combinations as possible predictive factors for identification of individuals with increased risk of formation and development of adenocarcinoma (AC) and squamous cell carcinoma (SCC) of lung in Slovak population. The study was conducted on 520 individuals consisting of 118 patients with adenocarcinoma, 112 patients with squamous cell carcinoma and 290 control individuals. ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604975 Thu, 19 Jan 2012 04:30:02 +0100 5604975 http://www.medworm.com/index.php?rid=5604974&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248274%26dopt%3DAbstract Authors: Cechova H, Lassuthova P, Novakova L, Belickova M, Stemberkova R, Jencik J, Stankova M, Hrabakova P, Pegova K, Zizkova H, Cermak J Abstract Epigenetic de novo methylation of CpG islands is an important event in malignant transformation. Two genes are frequently methylated: cyclin-dependent kinase inhibitor 2B (CDKN2B) and cyclin-dependent kinase inhibitor 2A (CDKN2A). In our study methylation of these genes was studied in 63 patients with myelodysplastic syndromes (MDS), 2 with myelodysplastic/myeloproliferative neoplasms (MDS/MPN) and 13 with acute myeloid leukemia (AML). Five patients were monitored during 5-azacytidine treatment. Twenty-six healthy donors were tested in a control group. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) met... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604974 Thu, 19 Jan 2012 04:30:02 +0100 5604974 http://www.medworm.com/index.php?rid=5604973&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248275%26dopt%3DAbstract The significance of portal vein embolization in the treatment of colorectal liver metastases. Neoplasma. 2012;59(2):175-82 Authors: Zboril P, Vyslouzil K, Klementa I, Skalicky P, Vomackova K, Cerna M, Cwiertka K Abstract The first aim of the present paper was to evaluate hypertrophy of liver parenchyma after portal vein embolization in patients after systemic chemotherapy for colorectal carcinoma metastases and planned extensive liver resections. The second aim was to study whether hypertrophy of the liver parenchyma remnant after could influence the postoperative course large liver resections in long-term chemotherapy within complex therapy of colorectal carcinoma.The prospective study comprised of 43 patients with colorectal hepatic metastases in whom liver resections of ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604973 Thu, 19 Jan 2012 04:30:02 +0100 5604973 http://www.medworm.com/index.php?rid=5604972&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248276%26dopt%3DAbstract Authors: Sivgin S, Baldane S, Kaynar L, Kurnaz F, Pala C, Ozturk A, Cetin M, Unal A, Eser B Abstract Iron overload increases the risk of infections, veno-occlusive disease and hepatic dysfunction in post-transplant period. Our objective was to investigate the association of pre-transplant ferritin levels with complications and survival after allogeneic hematopoietic stem cell transplantation (alloHSCT).We retrospectively analysed 84 patients' data who had undergone allogeneic HSCT into two groups: patients with a serum ferritin level≥1000ng/ml, and patients with <1000 ng/ml at the time of HSCT.Cox-regression analysis showed that pre-transplant serum ferritin levels were significantly higher in patients who had at least one infectious event compared with those who had no an... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604972 Thu, 19 Jan 2012 04:30:02 +0100 5604972 http://www.medworm.com/index.php?rid=5604971&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248277%26dopt%3DAbstract Authors: Gumulec J, Masarik M, Krizkova S, Hlavna M, Babula P, Hrabec R, Rovny A, Masarikova M, Sochor J, Adam V, Eckschlager T, Kizek R Abstract Current diagnostic techniques are inefficient in distinguishing latent and low-risk forms of prostate cancer from high-risk forms. The present study is focused on determination of putative tumor markers of aggressive high-grade forms of prostate cancer. Potential markers were determined in blood sera of 133 patients (82 cases and 51 controls) and in cell lines (Gleason score 9-derived 22Rv1 and normal tissue derived PNT1A) on mRNA and protein levels. Alpha-methylacyl-CoA racemase (AMACR), metallothionein classes 1A and 2A (MT1A and MT2A) were determined and compared to prostate specific antigen (PSA) levels. On mRNA level, significantly i... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604971 Thu, 19 Jan 2012 04:30:02 +0100 5604971 http://www.medworm.com/index.php?rid=5604970&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248278%26dopt%3DAbstract Authors: Gao L, Asmitanand T, Ren H, Wu F, Zhang Y, Li X, DI L, Song Z, Yang T, Chen T, Merrilees M, Wu L, Chen M Abstract White light bronchoscopy [WLB] has been used for identification and localization of intra-epithelial pre-neoplastic and neoplastic lesions within the bronchus. Aim of the study was to evaluate the uses of WLB to detect and localize the precancerous and cancerous lesions, and in addition to analyze morphologic presentation, and association to histological type and the variation between genders.A total of 4983 patients were examined by WLB from 2004 to 2009 in a local tertiary teaching hospital. The following parameters were collected: morphological presentation, biopsy sites, histology. The patients' records of age, sex, smoking status, blood-gas, X-RAY/CT, CB... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604970 Thu, 19 Jan 2012 04:30:02 +0100 5604970 http://www.medworm.com/index.php?rid=5604969&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248279%26dopt%3DAbstract Authors: Wu J, Wu X, Lian K, Lin B, Guo L, Ding Z Abstract Human ether à go-go (hEAG) potassium channels are primarily expressed in brain but also frequently overexpressed in solid tumors, which could indicate their potential value for cancer diagnosis and therapy. hEAG1, one member of the hEAG subfamily, has been shown to play a role in neoplastic process. Here we report the expression of hEAG1 in human osteosarcoma detected by a new polyclonal antibody. The full-length hEAG1 cDNA was cloned from human osteosarcoma cell line MG63 by RT-PCR and expressed in Escherichia coli as His tagged protein. The 6His-hEAG1F protein was purified by nickel agarose and used as the antigen to immunize rabbits following standard protocols. The obtained antiserum could detect hEAG1 exogenously ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604969 Thu, 19 Jan 2012 04:30:02 +0100 5604969 http://www.medworm.com/index.php?rid=5604968&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248280%26dopt%3DAbstract Authors: Yang W, Cui S, Ma J, Lu Q, Kong C, Liu T, Sun Z Abstract Genomic, epigenetic and expression alterations of WW domain containing oxidoreductase (WWOX) have been implicated in multiple tumor types. The current study was designed to examine the expression of WWOX in tumor tissues of human bladder transitional cell carcinoma (BTCC) and the influence of cigarette smoke extract (CSE) on WWOX expression and methylation status in T-24 bladder cancer cells. WWOX protein expression was evaluated by immunohistochemistry staining in a series of tumor samples from 78 patients with BTCC and 26 normal bladder tissues. The expression level and methylation status of WWOX in CSE-treated cells were examined by using quantitative Real-Time RT-PCR and methylation specific PCR, respectively. ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604968 Thu, 19 Jan 2012 04:30:02 +0100 5604968 http://www.medworm.com/index.php?rid=5604967&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248281%26dopt%3DAbstract Authors: Nagasaki S, Nakamura Y, Maekawa T, Akahira J, Miki Y, Suzuki T, Ishidoya S, Arai Y, Sasano H Abstract Gastrin-releasing peptide (GRP) belongs to the family of bombesin-like peptides. GRP was demonstrated to stimulate the proliferation and invasiveness of androgen-independent prostate carcinoma. GRP mediates its action through the membrane-bound receptor, GRP receptor (GRPR), which is characterized by a high-affinity binding for both GRP and bombesin. In human prostate cancer tissue, GRPR mRNA was reported to be detectable in more than 90% but its immunolocalizaition has not been reported. Therefore, in this study we immunolocalized GRPR in 51 human prostate cancer cases and correlated the findings with several clinicopathological parameters in order to beter understand t... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604967 Thu, 19 Jan 2012 04:30:02 +0100 5604967 http://www.medworm.com/index.php?rid=5604966&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248282%26dopt%3DAbstract In conclusion, the response rate, median PFS and median OS are similar in both arms, while the mDCF regimen are more favorable than the DCF for toxicity profile regimen in advanced gastric cancer patients who were undergoing first-line palliative treatment. Therefore, a prospective and larger clinical trials are needed. Keywords: advanced gastric cancer, docetaxel, cisplatin, fluorouracil. PMID: 22248282 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5604966 Thu, 19 Jan 2012 04:30:02 +0100 5604966 http://www.medworm.com/index.php?rid=5442914&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103896%26dopt%3DAbstract Authors: Adamkov M, Kajo K, Vybohova D, Krajcovic J, Stuller F, Rajcani J Abstract The antiapoptotic protein survivin is widely expressed in most human cancers, including carcinomas of the breast. It is rarely detected in corresponding normal adult tissues. Therefore, survivin comes into the limelight as a promising diagnostic biomarker and prognostic parameter. Immunohistochemically, we examined the expression of this protein in 126 cases of ductal breast carcinoma to determine the association with clinicomorphological parameters such as age of patients, grade, stage and size of the primary tumor, lymph node metastasis, vascular invasion as well as estrogen and progesterone status. In each section, the subcellular location of survivin antigen, the intensity of staining and the p... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442914 Fri, 25 Nov 2011 12:25:42 +0100 5442914 http://www.medworm.com/index.php?rid=5442913&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103897%26dopt%3DAbstract This study was aimed to establish clinical efficacy and tolerability of gemcitabine and cisplatin combination in patients with metastatic triple negative breast cancer progressing after anthracycline and taxane based chemotherapies.Thirty-three patients who were given cisplatin and gemcitabine for triple negative and metastatic breast cancer were evaluated retrospectively. A total of 141 cycles were administered with a median 4 cycles per patient. Median follow-up time was 14 months (range, 2-36 months). Objective response rate was 27.3%. Total clinical benefit of the combination was 48.4%. The estimated median progression free survival and median overall survival were 5 months and 14 months, respectively. The most common Grade 3 and 4 toxicity were neutropenia and thrombocytopenia observe... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442913 Fri, 25 Nov 2011 12:25:32 +0100 5442913 http://www.medworm.com/index.php?rid=5442912&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103898%26dopt%3DAbstract Authors: Hudecova A, Hasplova K, Kellovska L, Ikreniova M, Miadokova E, Galova E, Horvathova E, Vaculcikova D, Gregan F, Dusinska M Abstract Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single- and double- strand breaks, as well as a DNA base loss resulting in apurinic/apyrimidinic (AP) sites. The aim of this study was to induce an adaptive response (AR) by zeocin in freshly isolated human lymphocytes from blood and to observe whether plant extracts could modulate this response. The AR was evaluated by the comet assay. The optimal conditions for the AR induction and modulation were dete... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442912 Fri, 25 Nov 2011 12:25:22 +0100 5442912 http://www.medworm.com/index.php?rid=5442911&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103899%26dopt%3DAbstract Long-term trends in the development of the epidemiology of breast cancer in the Slovak and Czech Republic with reference to applied screening and international comparisons. Neoplasma. 2012;59(1):70-8 Authors: Ondrusova M, Muzik J, Durdik S, Ondrus D Abstract Breast cancer represents a major problem in oncology and epidemiology, especially because of the growing trends in its incidence, which are most pronounced in countries with historically low levels of incidence of this disease and because of the increasingly unfavorable mortality trends even in some countries where screening has been established. The purpose of this study is to analyse the incidence of breast cancer and resulting mortality in two neighbouring countries with national population-based cancer data in cen... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442911 Fri, 25 Nov 2011 12:25:12 +0100 5442911 http://www.medworm.com/index.php?rid=5442910&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103900%26dopt%3DAbstract Microsomal epoxide hydrolase polymorphisms, cigarette smoking and prostate cancer risk in the Slovak population. Neoplasma. 2012;59(1):79-84 Authors: Sivonova MK, Dobrota D, Matakova T, Dusenka R, Grobarcikova S, Habala V, Salagovic J, Tajtakova M, Pidanicova A, Valansky L, Lachvacs L, Kliment J, Nagy V, Kliment J Abstract Polymorphisms in tobacco carcinogen metabolizing enzymes may generate interindividual variations towards the risk of developing prostate cancer. One of these enzymes is microsomal epoxide hydrolase (EPHX1) which metabolizes polycyclic aromatic hydrocarbons, or PAH, carcinogens found in cigarette smoke. The activity of this enzyme is affected by two polymorphisms, a substitution of Tyr113 by His in exon 3 and a substitution of His139 by Arg in exon 4. ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442910 Fri, 25 Nov 2011 12:25:02 +0100 5442910 http://www.medworm.com/index.php?rid=5442909&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103901%26dopt%3DAbstract In this study we investigated the level of methylation in the promoter region of BRCA1 and its correlation with clinico-pathological and molecular characteristics in a group of 135 Bulgarian patients. Methylation specific PCR was applied to determine methylation status of tumor samples. Clinical impact of BRCA1 hypermethylation was estimated using standard statistical methods including Fisher's exact and the Chi-squared tests, the Kaplan-Meier method, the univariate and multivariate Cox proportional hazards regression model. We found that hypermethylation was present in 17.04% of the cases (23/135). Patients with hypermethylation in BRCA1 displayed favorable clinical status as their tumors were smaller in size (P = 0.066), lacked p53 gene mutations (P = 0.073) and were of lobular type (P... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442909 Fri, 25 Nov 2011 12:24:52 +0100 5442909 http://www.medworm.com/index.php?rid=5442908&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103902%26dopt%3DAbstract This study evaluated the properties of adrenal lesions in cancer and non-cancer patients and investigated what variables may help predict adrenal metastasis. This retrospective study used 18fluorodeoxyglucose PET/CT on 371 patients with adrenal lesions (N = 260 with a primary tumor and N = 111 with an unknown primary tumor). Parameters such as the presence of a tumor, nodule, enlarged adrenal, maximum standardized uptake (SUVmax ratio) were evaluated. Univariate and multivariate analysis were used to identify variables that may predict risk of adrenal metastasis. Subjects with adrenal metastasis versus those without had a higher frequency of primary lung tumors (53.7% versus 28.6%, respectively; P ≤ 0.001) but a lower frequency of gastrointestinal cancer (9.3% versus 20.4%, r... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442908 Fri, 25 Nov 2011 12:24:42 +0100 5442908 http://www.medworm.com/index.php?rid=5442907&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103903%26dopt%3DAbstract Authors: Lu HY, Sun WY, Chen B, Zhang YP, Cai JF, Su D, Wang Z, Zheng YQ, Ma SL Abstract To know the incidence of epidermal growth factor receptor (EGFR) mutations in small cell lung cancer (SCLC) patients who received surgical resection in mailand China. xTAG technology was used to detect the EGFR exon 19 and exon 21 mutations of 40 patients with SCLC who received surgical treatment in Zhejiang Cancer Hospital from 1998 to 2010. 2 of 40 cases were found with mutations in exon 19 of the EGFR gene. The mutation in exon 19 of the EGFR gene is in a female and non smoking patient which pathology is SCLC combined adenocarcinoma, and the other is male and smoking patient which pathology is SCLC combined squamous cell carcinoma. The EGFR mutation is rare in SCLC patients, and EGFR mutatio... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442907 Fri, 25 Nov 2011 12:24:32 +0100 5442907 http://www.medworm.com/index.php?rid=5442906&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103904%26dopt%3DAbstract In conclusion, our findings demonstrate that silencing of EphA2 inhibits gastric cancer SGC-7901 cell proliferation, invasion and MMP-9 expression, which indicate that the specific inhibition of EphA2 may be a potential approach for gastric cancer therapy. Keywords: EphA2, gastric cancer, MMP-9, SGC-7901, siRNA, tumor invasion. PMID: 22103904 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442906 Fri, 25 Nov 2011 12:24:22 +0100 5442906 http://www.medworm.com/index.php?rid=5442905&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22103905%26dopt%3DAbstract Authors: Dworzecki T, Idasiak A, Syguła D, Dworzecka U, Suwiński R Abstract The aim of this study is to present evaluation of treatment toxicity and the rate of local control in non-small cell lung cancer patients (NSCLC) treated with stereotactic body radiotherapy (SBRT). The analysis was performed on heterogenous group of 61 NSCLC patients, treated with SBRT between 2005 and 2008. It included 26 patients in clinical stage I, 5 in stage II, 22 in III and 8 in stage IV. In 30 patients SBRT was the only treatment, in 20 patients SBRT was a salvage therapy and in 11 patients SBRT was used as a boost after conventionally fractionated radiotherapy (CRT). The mean age was 67 yrs. Fifteen patients received chemotherapy in the course of treatment. Radiation doses were converted into... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5442905 Fri, 25 Nov 2011 12:24:12 +0100 5442905 http://www.medworm.com/index.php?rid=5424061&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22082308%26dopt%3DAbstract Authors: Mazurek AM, Olbryt M Abstract Neuropilin-1 (Nrp1), originally characterized as an adhesion molecule in the nervous system, is a co-receptor for class-3 semaphorins. Neuropilins and semaphorins are highly expressed in a wide spectrum of tumors and have been shown to influence their growth and vascularization. Despite the growing body of data on neuropilin/semaphorin regulation of tumor growth, still the exact mechanism of their activity remains to be elucidated. Previously published data suggests that Nrp1 has both anti- and promigratory characteristics in different tumor types, although no data is available on its role in melanoma cells. In this paper, we studied the effect of Nrp1 downregulation on B16(F10) melanoma cells migration. Our results show that the silencing... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5424061 Fri, 11 Nov 2011 05:00:00 +0100 5424061 http://www.medworm.com/index.php?rid=5424060&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22082309%26dopt%3DAbstract This study aims to investigate the expression of pSTAT3, VEGF and VEGF-C in pancreatic adenocarcinoma and their relations to the clinicopathological features, tumor angiogenesis and prognosis. In the present study, the expression of pSTAT3, VEGF and VEGF-C and microvascular density (MVD) were examined via immunohistochemistry. The clinicopathological information was collected and patients were regularly followed up. The relationship between the parameters and the clinicopathological features were analyzed, and the univariate and multivariate prognostic factors were also analyzed. The expression of pSTAT3 in tumor tissues was significantly higher in contrast to that in normal tissues, and pSTAT3 was related to VEGF and VEGF-C expression, MVD, tumor size, lymphogenous status and TNM staging ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5424060 Fri, 11 Nov 2011 05:00:00 +0100 5424060 http://www.medworm.com/index.php?rid=5346443&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22017589%26dopt%3DAbstract Authors: Deng LL, Shao YX, Lv HF, Deng HB, Lv FZ Abstract The death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in tumor cells. But studies have demonstrated that many tumor cells were resistant to TRAIL-induced apoptosis. CYLD is recognized as a negative regulator of nuclear factor-kappa B (NF-κB) activity. To explore a correlation between CYLD expression and responsiveness to TRAIL in lung cancer cell lines, we established lung cancer cell lines that stably express CYLD. Our data provided the first evidence that increased expression of CYLD directly blocks TRAIL-induced NF-κB activation, and consequently increases TRAIL-induced apoptosis in lung cancer cells. CYLD may act as a therapeutic target of lung cancer.... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5346443 Thu, 20 Oct 2011 04:00:00 +0100 5346443 http://www.medworm.com/index.php?rid=5346442&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22017590%26dopt%3DAbstract Authors: Zhou LT, Liu FY, Li Y, Peng YM, Liu YH, Li J Abstract Cancer is a complex disease with interactions between normal and neoplastic cells. Since current therapies for cancer largely rely on drugs or radiation that kill dividing cells or block cell division, these treatments may have severe side effects on normal proliferating cells in patients with cancer. Recently, immunotherapeutic approaches for cancer therapy, by which monoclonal antibodies (Mabs) target tumor specific antigens, have shown great potential. Glycoprotein non-metastatic melanoma protein B (Gpnmb)/Osteoactivin (OA) is a transmembrane glycoprotein highly expressed in various types of cancer. Gpnmb/OA promotes the migration, invasion and metastasis of tumor cells. CR 011-vcMMAE is a Mab-drug conjugate ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5346442 Thu, 20 Oct 2011 04:00:00 +0100 5346442 http://www.medworm.com/index.php?rid=5346441&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22017591%26dopt%3DAbstract Authors: Janikova M, Skarda J Abstract Cancer stem cells (CSCs) share many features with embryonic stem cells (ESCs) such as the ability for self-renewal and differentiation. Signaling pathways that are involved in these processes are also involved in chemo- and radioresistance (e.g. Wnt, Notch and Hedgehog pathways). This review is focused on the influence of three important differentiation pathways on carcinogenesis and on chemo- and radioresistance in ESCs and CSCs. Keywords: Stem cell, Chemoresistance, Radioresistance, Wnt pathway, Notch pathway, Hedgehog pathway. PMID: 22017591 [PubMed - as supplied by publisher] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=5346441 Thu, 20 Oct 2011 04:00:00 +0100 5346441 http://www.medworm.com/index.php?rid=5220103&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895398%26dopt%3DAbstract Authors: Zavesky L, Jancarkova N, Kohoutova M Abstract Ovarian cancer representing the most lethal gynecologic malignancy escapes from the efforts to manage the disease. We reviewed the current state of the research considering three main concepts on origin of ovarian cancer including epithelial-mesenchymal transition, secondary origin from Müllerian system and cancer stem cell hypothesis. Cytogenetic and molecular characteristics of ovarian cancer are focused particularly on microRNA expression studies revealing huge potential in recent years, although other transcriptomic, proteomic, epigenetic, epidemiologic and immunological factors are touched upon, too. Routine and investigated diagnostic and treatment methods are outlined and several factors revealed to be associated with p... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220103 Thu, 15 Sep 2011 12:16:02 +0100 5220103 http://www.medworm.com/index.php?rid=5220102&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895399%26dopt%3DAbstract Authors: Petera J, Sirak I, Beranek M, Vosmik M, Drastikova M, Paulikova S, Soumarova R Abstract Radical radiotherapy with concurrent cisplatin-based chemotherapy is an established treatment for cervical cancer patients with stage FIGO IIB and higher. The tumor control can be achieved in 40-80% of patients, the treatment is associated with the risk of late postiradiation complications in 10 - 15% of cases. Detection of the factors predictive for tumor control and late morbidity is a possible direction how to individualize radiotherapy dose and technique. The aim of our review is to summarize results of studies inquiring various molecular markers predicting tumor response to radiotherapy and a risk of late complications. A lot of candidate molecules were evaluated in histochem... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220102 Thu, 15 Sep 2011 12:16:02 +0100 5220102 http://www.medworm.com/index.php?rid=5220101&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895400%26dopt%3DAbstract Authors: Smetana K, Karhan J, Trneny M Abstract The present study was undertaken to provide complementary information on heterochromatin condensation in central and peripheral nuclear regions during maturation of human leukemic lymphocytes using simple image processing and DNA image densitometry at the single cell level. Such approach indicated that the heterochromatin condensation in perinucleolar and extranucleolar "gene rich" central nucleolar regions preceded that in the "gene poor" nuclear periphery at the nuclear membrane. Thus, the maturation of lymphocytes was accompanied by a marked increase of the heterochromatin condensation at the nuclear membrane that reflected the maturity of these cells. In addition, in contrary to the nuclear size, no substantial differences of th... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220101 Thu, 15 Sep 2011 12:16:02 +0100 5220101 http://www.medworm.com/index.php?rid=5220100&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895401%26dopt%3DAbstract In this study we revealed for the first time that metformin treatment led to increased apoptosis in human lung cancer cell lines A549 and NCI-H1299 and significantly inhibited the cells proliferation in a dose- and time-dependent manner, which was further demonstrated by the data obtained from A549 tumor xenografts in nude mice. We also found that metformin treatment can activate AMP-activated protein kinase, JNK/p38 MAPK signaling pathway and caspases, as well as upregulate the expression of growth arrest and DNA damage inducible gene 153 (GADD153). Either blockade of JNK/p38 MAPK pathway or knockdown of GADD153 gene abrogated the apoptosis-inducing effect of metformin. Taken together, our data suggest that metformin inhibits the growth of lung cancer cells and induces apoptosis through... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220100 Thu, 15 Sep 2011 12:16:02 +0100 5220100 http://www.medworm.com/index.php?rid=5220099&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895402%26dopt%3DAbstract Authors: Yu LR, Lv JQ, Jin LY, Ding SD, Ma XY, Wang JJ, Zhu XQ Abstract Protein kinase C was found to be significantly over-expressed in cancer samples compared to adjacent normal cervical tissues by proteomics in our previous study. The aim of this study was to examine protein kinase C expression and to analyze the expression patterns of protein kinase C isoforms in squamous cervical cancer at the protein levels and their associations with clinical and pathologic factors of squamous cervical cancer. First, Western blotting was used to examine protein kinase C expression in the specimens of tumors and matched adjacent normal tissues which were collected from 12 patients with squamous cervical cancer. Protein kinase C isoforms (α, δ, θ and ζ) expression were then detec... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220099 Thu, 15 Sep 2011 12:16:02 +0100 5220099 http://www.medworm.com/index.php?rid=5220098&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895403%26dopt%3DAbstract Authors: Scudla V, Petrova P, Minarik J, Pika T, Bacovsky J Abstract The aim of the study was to analyze differences in the serum levels of 8 selected biological parameters between monoclonal gammopathy of undetermined significance (MGUS) and different stages of multiple myeloma (MM), potentially beneficial for distinguishing between the two conditions. The analyzed group of 131 subjects comprised 62 individuals with MGUS and 69 MM patients examined at the time of diagnosis. The serum levels were determined by a quantitative immunoradiometric assay (insulin-like growth factor 1, IGF-1) and quantitative sandwich enzyme immunoassay (osteopontin, OPN; endostatin, ES; macrophage inflammatory proteins 1α/β, MIP-1α/β; angiogenin, ANG; and interleukin 17, IL-17). The analysis showed a... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220098 Thu, 15 Sep 2011 12:16:02 +0100 5220098 http://www.medworm.com/index.php?rid=5220097&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895404%26dopt%3DAbstract Authors: Tarnawski R, Michalecki L, Blamek S, Hawrylewicz L, Piotrowski T, Slosarek K, Kulik R, Bobek-Billewicz B Abstract Prophylactic cranial irradiation (PCI) is performed on patients with limited or extensive small-cell lung cancer to reduce incidence of brain metastases and prolong survival. PCI may induce neurocognitive impairment. Decreasing irradiation of neural stem cells (NSC) might reduce PCI-induced toxicity. We tested the feasibility of reducing irradiation doses to neural stem cell (NSC) regions while maintaining prescribed doses to the planned target volume (PTV). Irradiation plans utilizing intensity-modulated radiotherapy (IMRT), helical TomoTherapy, and RapidArc for 10 consecutive lung cancer patients were evaluated. The dose distribution, dose-volume histograms, ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220097 Thu, 15 Sep 2011 12:16:02 +0100 5220097 http://www.medworm.com/index.php?rid=5220093&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895405%26dopt%3DAbstract Authors: Kozics K, Valovicova Z, Slamenova D Abstract Flavonoids are plant derivatives of flavone of which chemical structure is characterized by various degrees of hydroxylation and glycosidic substitution. In the present study we investigated the protective effect of two structurally different groups of flavonoids against-benzo[a]pyrene (B(a)P)-induced genotoxic effects on human hepatocellular carcinoma (HepG2) cells. The first group of flavonoids: fisetin, kaempferol, galangin, quercetin, and luteolin, hydroxylated at the 3´,4´-position on the B ring, 3 - position of C ring and on the A ring was able to inhibit significantly B(a)P-induced genotoxic effects in a greater degree than the second group of flavonoids: chrysin, 7-hydroxyflavone, 7,8-dihydroxyflavone and baica... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220093 Thu, 15 Sep 2011 12:16:02 +0100 5220093 http://www.medworm.com/index.php?rid=5220092&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895406%26dopt%3DAbstract In this study we evaluated the effect of leukemia cell lines on nemosis-induced COX-2 expression in stromal fibroblasts. We found that THP-1 cells were the most potent leukemic cells (IC50=746) to suppress COX-2 expression. The U-937 cell line exhibited similar suppressive potency (IC50=921), whereas the KG-1 cell line (IC50=3519) was the least potent to affect COX-2 expression in the stromal cells. Our study shows that human leukemic cells can actively participate in modulation of stromal inflammation via inhibition of COX-2 expression. In a co-culture model of leukemia cell lines and stromal fibroblasts, our data suggest that the tumor-stromal interactions are complexly regulated, and the straightforward association of COX-2 expression with tumor progression may require re-evaluation s... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220092 Thu, 15 Sep 2011 12:16:02 +0100 5220092 http://www.medworm.com/index.php?rid=5220091&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895407%26dopt%3DAbstract Authors: Guo RX, Zhang RF, Wang XY, Li LX, Shi HR, Qiao YH Abstract Why estrogen hyperstimulation can lead to endometrial carcinogenesis has not been fully clear yet. Non-nuclear action of estrogen has arised much attention of many experts. Signal transducer and activator of transcription 3 is a very important signal molecule, which plays vital role in endometrial canver. The present study is oriented to the problem whether estrogen can activate STAT3 by non-nuclear action in endometrial cancer cells. So, the levels of phosphorylated STAT3 (P-STAT3) and total STAT3 were examined by western blot in endometrial cancer cells including Ishikawa with rich-expressed estrogen receptor (ER) and HEC-1A with poor-expressed ER after stimulation with 1μM estradiol (E2) at different time poi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220091 Thu, 15 Sep 2011 12:16:02 +0100 5220091 http://www.medworm.com/index.php?rid=5220090&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895408%26dopt%3DAbstract In this study, short hairpin RNA targeting STAT3 was cloned into pGenesil-2 plasmid vector and the effects of STAT3 silencing in 4T1 breast cancer cells were analyzed both in vitro and in vivo. Forty-eight hours after transfecting with pSi-STAT3, the expression level of STAT3, the upstream regulator and downstream targets were measured using Western blot. Moreover, the effects of pSi-STAT3 on migration and invasion in 4T1 cells were tested using wound-healing and tube formation assay. Furthermore, 4T1 subcutaneous mice model was used to evaluate the effects of pSi-STAT3 on tumor growth and metastasis. Proliferation, apoptosis, angiogenesis in tumor tissues and lung metastases were measured by PCNA, TUNEL, and CD31 immunostaining, respectively. Our results indicated that siRNA targeting STA... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220090 Thu, 15 Sep 2011 12:16:02 +0100 5220090 http://www.medworm.com/index.php?rid=5220089&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895409%26dopt%3DAbstract Authors: Strhakova L, Bujalkova MG, Hojsikova I, Lukackova R, Behulova R, Mistrik M, Repiska V Abstract The presence of BCR-ABL oncogene mutations in patients with chronic myeloid leukemia (CML) may be responsible for the failure of tyrosine kinase inhibitor (TKI) treatment. The aim of the study was to evaluate the frequency of BCR-ABL gene mutations in patients with CML treated with tyrosine kinase inhibitors. Our lab received 64 samples (34 women, 30 men) from patients with CML who failed or had suboptimal response to TKI treatment. The mutation analysis was performed in 61 patients with CML, 3 patients could not be tested because of inadequate RNA quality. An 866 base pair fragment containing the ABL kinase domain was amplified in a seminested RT (reverse transcriptase)-PCR an... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220089 Thu, 15 Sep 2011 12:16:02 +0100 5220089 http://www.medworm.com/index.php?rid=5220088&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895410%26dopt%3DAbstract Authors: Al-Sahmani M, Trnavska I, Sevcikova S, Antosova M, Antosova L, Kissova J, Adam Z, Pour L, Nemec P, Greslikova H, Krejci M, Zahradova L, Bulikova A, Penka M, Hajek R Abstract Multiple myeloma (MM) is a hematological malignancy caused by clonal proliferation of malignant plasma cells (PC). The aim of the work is to determine prognostic significance of morphological subtypes of PC in relation to overall treatment response, long-term survival and other conventional prognostic parameters. One hundred and thirty-nine newly diagnosed MM patients who underwent autologous transplantation in clinical trials conducted in one center were included. Percentual representation of subtypes of plasma cells in bone marrow was measured based on progressive analysis of nucleolus, nuclear chr... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5220088 Thu, 15 Sep 2011 12:16:02 +0100 5220088 http://www.medworm.com/index.php?rid=5062642&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744988%26dopt%3DAbstract Authors: Kucerova L, Kovacovicova M, Polak S, Bohac M, Fedeles J, Palencar D, Matuskova M Human adipose tissue was shown to be a very attractive source of mesenchymal stromal cells that have a wide scale of potential applications in reconstructive plastic surgery and regenerative medicine. However, these cells were described to have profound effects on biological behaviour of tumour cells. The aim of this study was to analyze the influence of adipose tissue-derived human mesenchymal stromal cells (AT-MSC) on the proliferation of breast cancer cells. We have tested proliferation of three different human breast cancer cell lines under the influence of AT-MSC derived soluble factors as well as in the direct cocultures. These data were supplemented with the expression analysis of cytokines... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062642 Tue, 26 Jul 2011 02:00:05 +0100 5062642 http://www.medworm.com/index.php?rid=5062641&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744989%26dopt%3DAbstract Authors: Chen A, Shen Y, Xia M, Xu L, Pan N, Yin Y, Miao F, Shen C, Xie W, Zhang J HLA-G and HLA-E are nonclassical human MHC class I molecules, which promote tolerance to NK cytotoxicity. MICA and MICB are known to enhance the functions of NK and T cells. However, the expression of these molecules has never been investigated in liver cancer. <br />Using RT-PCR and western blot, we aimed to identify the expression of HLA-G, HLA-E, MICA and MICB in a panel of 41 tissues dissecting from liver cancer patients in China. <br />HLA-G mRNA was expressed in 8 of 41 Human Hepatocellular Carcinoma (HCC) specimens and in 1 adjacent normal hepatocellular tissue. The expression of HLA-G protein was found in 7 of the 8 HLA-G mRNA-positive HCC tissues. HLA-E mRNA was up-regulated in... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062641 Tue, 26 Jul 2011 02:00:05 +0100 5062641 http://www.medworm.com/index.php?rid=5062640&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744990%26dopt%3DAbstract Authors: Halamkova J, Kiss I, Pavlovsky Z, Tomasek J, Jarkovsky J, Cech Z, Tucek S, Hanakova L, Moulis M, Zavrelova J, Man M, Benda P, Robek O, Kala Z, Penka M Urokinase (uPA) plays an essential role in the activation of plasminogen to plasmin, and together with its receptor (uPAR), tissue activator (tPA) and urokinase inhibitors (PAI 1, PAI 2, PAI 3 and protease nexin) forms the plasminogen activator system (PAS), a component of metastatic cascade importantly contributing to the invasive growth and angiogenesis of malignant tumours. In our project we examined the expression of uPA, uPAR, PAI 1 and PAI 2 in tumor tissue and we also studied the plasma levels of PAI 1 before and after the initiation of therapy in patients with colorectal carcinoma in relationship to grade of tumor and ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062640 Tue, 26 Jul 2011 02:00:05 +0100 5062640 http://www.medworm.com/index.php?rid=5062639&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744991%26dopt%3DAbstract Authors: Hasplova K, Hudecova A, Miadokova E, Magdolenova Z, Galova E, Vaculcikova L, Gregan F, Dusinska M Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress and from various chronic diseases. Papaver rhoeas has a long history of medicinal usage, especially for ailments in adults and children. The possible cytotoxicity, genotoxicity and potential antioxidant effect of plant extract isolated from flowers of Papaver rhoeas was investigated in human lymfoblastoid cell line (TK6). Antioxidant activity of this extract was determined using the DPPH assay. The plant extract exhibited dose dependent free radical scavenging ability. The growth activity assay was used for determination of cytoto... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062639 Tue, 26 Jul 2011 02:00:05 +0100 5062639 http://www.medworm.com/index.php?rid=5062638&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744992%26dopt%3DAbstract Authors: Havranek O, Spacek M, Hubacek P, Mocikova H, Markova J, Trneny M, Kleibl Z Checkpoint kinase 2 gene (CHEK2) codes for an important mediator of DNA damage response pathway. Mutations in the CHEK2 gene increase the risk of several cancer types, however, their role in Hodgkin lymphoma (HL) has not been studied so far. The most frequent CHEK2 alterations (including c.470T>C; p.I157T) cluster into the forkhead-associated (FHA) domain-coding region of the CHEK2 gene. We performed mutation analysis of the CHEK2 gene segment coding for FHA domain using denaturing high-performance liquid chromatography in 298 HL patients and analyzed the impact of characterized CHEK2 gene variants on the risk of HL development and progression-free survival (PFS). The overall frequency of CHEK2 alter... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062638 Tue, 26 Jul 2011 02:00:05 +0100 5062638 http://www.medworm.com/index.php?rid=5062637&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744993%26dopt%3DAbstract The objective of this study was to evaluate the effect of blockage of STAT3 signaling pathway on IL-6 inducing EMT in human pancreatic cancer cells. We used SW1990 cells and induced them to undergo EMT by exposing these cells to soluble factor interleukin-6 (IL-6). The expression of Snail, E-cadherin, and Twist was detected by reverse transcription-PCR, real-time PCR, and Western blotting. Cell morphology was observed under invert phase-contrast microscope.The invasion ability was determined by cell invasion assay in vitro. Our results demonstrated that STAT3 signaling pathway was involved in pancreatic cancer cell invasion and EMT, and that EMT induced by IL-6 was associated with the activation of STAT3 signaling pathway. Inhibition of STAT3 signaling pathway by silencing of the STAT3 gen... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062637 Tue, 26 Jul 2011 02:00:05 +0100 5062637 http://www.medworm.com/index.php?rid=5062636&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744994%26dopt%3DAbstract Authors: Krejci M, Brychtova Y, Doubek M, Tomiska M, Navratil M, Racil Z, Dvorakova D, Horky O, Lengerova M, Pospisilova S, Mayer J Reduced-intensity conditioning (RIC) is widely used for allogeneic stem cell transplantation (SCT). Here we present our long-term experience with RIC regimen consisting of fludarabine (30 mg/m2/day on days -10 to -5), busulfan (4mg/kg/day on days -6 and -5) and antithymocyte globulin (ATG Fresenius, 10 mg/kg/day on days -4 to -1) (Flu-Bu-ATG) in a cohort of 71 patients with various hematological malignancies including chronic myeloid leukemia (24 patients), acute myeloid leukemia (19 patients), lymphoma (20 patients), multiple myeloma (3 patients), myelodysplastic syndrome (3 patients), and myelofibrosis (2 patients). The median age was 50 years. <br ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062636 Tue, 26 Jul 2011 02:00:05 +0100 5062636 http://www.medworm.com/index.php?rid=5062635&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744995%26dopt%3DAbstract Authors: Krskova L, Augustinakova A, Drahokoupilova E, Sumerauer D, Mudry P, Kodet R Rhabdomyosarcoma is the most common soft tissue sarcoma of childhood. There are two major histopathological types of RMS - embryonal (eRMS) and alveolar (aRMS). A molecular study of Igf2, MyoD1 and Myogenin was performed to determine the expression profiles and to assess the possible utility of these genes as potential treatment targets. Patients with RMS showed up to 100-fold increase of Igf2 transcription in comparison with normal skeletal muscle. Our data suggest that overexpression of Igf2 occurs in RMS of both histological subtypes. No correlation between the results of Igf2 mRNA expression and LOH at the 11p15 region (p= 0.12) was observed, but there was a trend of a higher expression of Ig... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062635 Tue, 26 Jul 2011 02:00:05 +0100 5062635 http://www.medworm.com/index.php?rid=5062634&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744996%26dopt%3DAbstract Authors: Lackovicova L, Banovska L, Bundzikova J, Janega P, Bizik J, Kiss A, Mravec B Both experimental and clinical data indicate that the sympathetic nervous system may affect the development of certain tumors. To test this, in the present study we combined in vivo and in vitro approaches to study the effect of the sympathetic nervous system on proliferation of BP6-TU2 fibrosarcoma cells. First, we investigated the effect of 6-hydroxydopamine-induced sympathectomy on tumor development and survival of tumor-bearing rats. One week after chemical sympathectomy, we injected the BP6-TU2 fibrosarcoma cells intraperitoneally into male Wistar rats. The sympathectomy significantly reduced the incidence of intraperitoneal tumors and resulted in significantly improved survival of tumor-bearing ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062634 Tue, 26 Jul 2011 02:00:05 +0100 5062634 http://www.medworm.com/index.php?rid=5062633&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744997%26dopt%3DAbstract Authors: Pluta P, Cebula-Obrzut B, Ehemann V, Pluta A, Wierzbowska A, Piekarski J, Bilski A, Nejc D, Kordek R, Robak T, Smolewski P, Jeziorski A Smac/DIABLO protein promotes caspase-dependent apoptosis by inhibition of inhibitor of apoptosis protein (IAP) family members. The role of Smac/DIABLO in breast cancer has not been yet established. Therefore, the aim of the study was to assess the expression of this protein in tumor cells from breast cancer patients. The expression of Smac/DIABLO was analyzed in 62 breast cancer patients by flow cytometry. The obtained results were compared with expression of this protein in benign breast tumor tissue, which served as the control (11 patients with fibroadenoma). Expression of caspase-3 proteins in breast cancer was also evaluated. Smac/DIABLO ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062633 Tue, 26 Jul 2011 02:00:05 +0100 5062633 http://www.medworm.com/index.php?rid=5062632&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744998%26dopt%3DAbstract Authors: Huang Y, Zhang HQ, Wang J, Song XG, Wang GH, Guan Q, Zho W, Huo F, Yu CZ, Zeng YJ Mammaglobin may be a potential serum biomarker for the differential diagnosis of breast cancer. 260 serum samples were collected from 127 untreated breast cancer patients and 133 healthy volunteers to analyze the sera expression of mammaglobin and its implications for both. The expression vector of pGEX-4T-2-Mammaglobin and pBVIL1-Mammaglobin were constructed and transformed into E.coli.HB101 for expression. The mice were immunized with the purified recombinant protein to prepare monoclonal antibody and to detect by ELISA the serum of normal people and breast cancer patients. Recombinant mammaglobin antigen was effectively expressed in E.coli. Two hybridoma cell lines were obtained after the mi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062632 Tue, 26 Jul 2011 02:00:05 +0100 5062632 http://www.medworm.com/index.php?rid=5062631&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21744999%26dopt%3DAbstract Authors: Tong JD, Jiao NL, Wang YX, Zhang YW, Han F Fibulin-3 gene has been identified as an antagonist of angiogenesis. We investigated the protein expression and promoter methylation status of fibulin-3 gene in colorectal cancer (CRC) and analyzed its correlation with clinicopathological factors. The study population enrolled 85 paired CRC specimens and adjacent normal tissues, as well as 32 cases of colorectal adenoma. Genomic DNA was extracted from paraffin-embedded samples using manual microdissection. Methylation-specific polymerase chain reaction (MSP) was used to determine the promoter methylation status and fibulin-3 gene expression was detected by immunohistochemistry. The results showed that, downregulation or silence of fibulin-3 protein was found in 57.6% (49/85) of CRC ti... Neoplasma journals http://www.medworm.com/rss/comments.php?id=5062631 Tue, 26 Jul 2011 02:00:05 +0100 5062631 http://www.medworm.com/index.php?rid=4766417&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520983%26dopt%3DAbstract Authors: Mladosievicova B, Dzurenkova A, Sufliarska S, Carter A The therapeutic response to thiopurines may result in either severe toxic or inadequate effect based on the interindividual genetic variability. Same drug doses of various anticancer drugs cause considerable interindividual differences in the therapeutic response. Genetic factors have a major impact on effectiveness of several anticancer drugs such as mercaptopurine, 5-fluorouracil, platinum agents, and cyclophosphamide. Heredity related differences in interindividual response to thiopurine therapy represent perhaps the most compelling evidence of pharmacogenomics' usefulness in identification of patients in risk for adverse drug reactions. A number of variations in the gene for thiopurine methyltransferase (TPMT) have b... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766417 Sat, 30 Apr 2011 04:45:15 +0100 4766417 http://www.medworm.com/index.php?rid=4766416&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520984%26dopt%3DAbstract Authors: Ondrusova M, Muzik J, Hrcka R, Friedova L, Ondrus D Slovak (SR) and Czech (CR) Republics reach up the highest recorded incidence rates of colorectal cancer. In analysis of the development and changes in colorectal cancer incidence in the above-mentioned countries, it was reported the most considerable incidence increase of the disease in males in the SR, then in males in the CR, subsequently in females in the SR and the slowest incidence rate was reported in females in the CR. Colorectal cancer mortality increased most rapidly in males in the SR, then in males in the CR, slower increase was reported in females in the SR and in females in the CR the mortality was in the long term stabilized. In both countries and both sexes clinical stage II is noted most frequently, also the d... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766416 Sat, 30 Apr 2011 04:45:15 +0100 4766416 http://www.medworm.com/index.php?rid=4766415&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520985%26dopt%3DAbstract Authors: Jamaspishvili T, Scorilas A, Kral M, Khomeriki I, Kurfurstova D, Kolar Z, Bouchal J Kallikrein-related peptidases 7 and 11 (KLK7/KLK11) share a high degree of structural similarity with PSA (KLK3) and other KLKs. The aim of this study was to evaluate differences in KLK7/ KLK11 expression in paired cancer/benign prostate foci and to determine possible associations with clinicopathological parameters. <br />Seventy archived paraffin-embedded tissue samples obtained from radical prostatectomy were stained for KLK7, KLK11, PSA and PSMA and expression was evaluated semiquantitatively. <br />The results showed statistically significant differences for all studied proteins between BPH and CaP foci. Both KLK7 (P=0.026) and KLK11 (P<0.001) expressions were decreased in ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766415 Sat, 30 Apr 2011 04:45:15 +0100 4766415 http://www.medworm.com/index.php?rid=4766414&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520986%26dopt%3DAbstract Authors: Oguztuzun S, Abu-Hijleh A, Coban T, Bulbul D, Kilic M, Iscan M, Iscan M The potential to metabolize endogenous and exogenous substances may influence breast cancer development and tumor growth. Therefore we investigated GST activity and the protein expression of glutathione S-transferases (GSTs) isoenzymes known to be involved in the metabolism of endogenous and exogenous carcinogens in breast cancer tissue to obtain new information on their possible role in tumor progression. <br />The interindividual variation in the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) and of 1,2-epoxy-3-(p-nitrophenoxy) propane (EPNP) with glutathione (GSH) by cytosolic glutathione S-transferases (GSTs) were investigated in human breast matched normal and tumor samples. The GSTA, GSTM, G... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766414 Sat, 30 Apr 2011 04:45:15 +0100 4766414 http://www.medworm.com/index.php?rid=4766413&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520987%26dopt%3DAbstract In conclusion, our results suggest that high levels of both VEGF-C and VEGF-D proteins are associated with lymph node involvement, and that VEGF-C expression is an independent predictor of risk for lymph node metastasis in esophageal cancer. In locally advanced disease, overexpression of VEGF-C and VEGF-D may be useful in identifying patients who are more likely to have a poor prognosis even after curative resection. Keywords: esophageal cancer, VEGF-C; VEGF-D, lymph node metastasis, prognosis. PMID: 21520987 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766413 Sat, 30 Apr 2011 04:45:15 +0100 4766413 http://www.medworm.com/index.php?rid=4766412&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520988%26dopt%3DAbstract Authors: Momekov G, Konstantinov S, Dineva I, Ionkova I Justicidin B produced by genetically transformed cultures of Linum leonii was tested for cytotoxic activity and induction of apoptosis in MDA-MB-231 and MCF-7 breast cancer derived cell lines. The tested lignan evoked strong, concentration dependent cytotoxicity in both cell lines, whereby MCF-7 proved to be far more sensitive as compared to MDA-MB-231. The 24 h treatment of both cell lines increased the level of apoptotic DNA fragmentation; however the proapoptotic activity is completely inhibited if the cells are co-incubated with the non-selective pan-caspase inhibitor Boc-Asp(OMe)-fluoromethyl ketone (PCI), which implies that justicidin B, activates programmed cell death via caspase -dependent mechanisms. Exposure of MDA-MB-... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766412 Sat, 30 Apr 2011 04:45:15 +0100 4766412 http://www.medworm.com/index.php?rid=4766411&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520989%26dopt%3DAbstract Authors: Katsibardi K, Papadakis V, Charisiadou A, Pangalis A, Polychronopoulou S The incidence, type and mortality of bacteremias were evaluated in a pediatric patient cohort, during the entire course of treatment for acute lymphoblastic leukemia (ALL). <br />Eighty-six patients with newly diagnosed ALL were studied. A bacteremic episode was defined as blood isolation of a pathogen in the presence of clinical symptomatology of septicaemia. Bacteremias were analyzed according to the treatment element being delivered and the degree of neutropenia. A central venous catheter (CVC) was inserted at diagnosis in all patients. <br />Fifty-two episodes of bacteremias were encountered in 38/86 (44%) patients, while 48/86 patients had no positive blood culture. Three out of the 3... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766411 Sat, 30 Apr 2011 04:45:15 +0100 4766411 http://www.medworm.com/index.php?rid=4766410&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520990%26dopt%3DAbstract Authors: Mello MF, Costa AF, Freitas LL, Soares AB, Araujo VC, Tincani AJ, Martins AS, Altemani A Nodal metastasis is an important prognostic indicator in head and neck cancers, including salivary carcinomas. In these, the risk for lymph node metastasis is variable and strongly associated with the tumor histologic type. The aim of the current study was to evaluate the lymphatic vessel density (LVD) and expressions of lymphangiogenic growth factors by tumor cells in different histologic types of salivary carcinomas subdivided according to the risk for nodal metastasis. In 15 high-risk (undifferentiated, high-grade mucoepidermoid and salivary duct carcinomas) and 60 low/moderate-risk tumors (adenoid cystic, low/intermediate-grade mucoepidermoid, acinic cell, myoepithelial, epithelial-myo... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766410 Sat, 30 Apr 2011 04:45:15 +0100 4766410 http://www.medworm.com/index.php?rid=4766409&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520991%26dopt%3DAbstract Authors: Poláková K, Bandžuchová E, Russ G HLA-G is a non-classical MHC class I antigen that functions as an immunomodulatory molecule. There are two forms of HLA-G antigens, soluble and membrane bound. Soluble HLA-G can be produced by translation of HLA-G transcripts (HLA-G5, -G6, -G7) and by shedding/proteolytic cleavage of membrane bound antigens (HLA-G1, -G2, -G3, -G4). Soluble as well as membrane bound HLA-G molecules have a direct inhibitory effect on immune responses. The relevance of soluble HLA-G in various pathologic conditions, such as transplantation, autoimmunity, infectious and malignant diseases, has been extensively investigated, however interpretation remains controversial.<br />In this work we analyzed the levels of sHLA-G (sHLA-G1 and HLA-G5) in differen... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766409 Sat, 30 Apr 2011 04:45:15 +0100 4766409 http://www.medworm.com/index.php?rid=4766408&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520992%26dopt%3DAbstract In conclusion, we demonstrated that blocking of Notch signaling by DAPT could inhibit the proliferation and invasion of human colorectal cancer LS174T cells and suggested that gamma-secretase inhibitors may provide a targeted therapy for MUC2-positive colorectal tumors. Keywords: cancer, colorectum, invasion, proliferation, Notch signaling. PMID: 21520992 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766408 Sat, 30 Apr 2011 04:45:15 +0100 4766408 http://www.medworm.com/index.php?rid=4766407&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520993%26dopt%3DAbstract Authors: Ozkaya S, Findik S, Atici AG, Dirica A Lung cancer continues to be the leading cause of cancer-related mortality and approximately 70% of patients present with locally advanced or metastatic disease at the time of diagnosis. More than 50% of lung cancer cases are diagnosed in patients over the age of 65 years. The doublet chemotherapies consisting of platinum plus one of the third-generation agents become currently the standard regimen, the first line chemotherapy The most of the available data regarding the optimal treatment of lung cancer comes from clinical trials in which the vast majority of patients are significantly younger than 65 years of age. <br />We aimed to investigate whether there is any difference in tolerability and efficacy in between adult(<65 years... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766407 Sat, 30 Apr 2011 04:45:15 +0100 4766407 http://www.medworm.com/index.php?rid=4766406&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21520994%26dopt%3DAbstract Authors: Wang D, Wang B, Zhai JX, Liu DW, Sun GG There were some studies on the associations between Glutathione S-transferase M1 (GSTM1) and Glutathione S-transferase T1 (GSTT1) polymorphisms and cervical cancer (CC) risk, but the results were inconsistent. Thus, a meta-analysis was performed. <br />The electronic databases PubMed, Science Direct, CBM, and CNKI were searched for possible studies. Finally, 16 studies (1,627 cases and 2,161 controls) were included. For the GSTM1 and GSTT1 polymorphisms, the unadjusted odds ratios (OR) and 95% confidence intervals (95% CI) from each study were used to estimate summary OR. Subgroup analyses by ethnicity and histological type of CC were also performed. <br />For the GSTM1 polymorphism, the null genotype of GSTM1 was associated... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766406 Sat, 30 Apr 2011 04:45:15 +0100 4766406 http://www.medworm.com/index.php?rid=4766405&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21524147%26dopt%3DAbstract In conclusion, PET/CT is a new standard in imaging the involvement of lymph nodes and extranodal organs in NHL patients regardless of their histopathological types. Both sensitivity and specificity of the examination are higher than those of CT as well as PET alone. Keywords: 18F-FDG PET - PET/CT - CT - non-Hodgkin's lymphoma - staging. PMID: 21524147 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4766405 Sat, 30 Apr 2011 04:45:15 +0100 4766405 http://www.medworm.com/index.php?rid=4600155&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391733%26dopt%3DAbstract Authors: Zurkova K, Chlanda P, Samkova Z, Babiarova K, Kutinova L, Krystofova J, Hainz P, Nemeckova S Therapeutic immunization with double recombinants of vaccinia virus (VACV) co-expressing sTβRII increased rejection of established TC-1 tumors in C57BL/6 mice in comparison with single recombinant expressing SigE7LAMP. Recombinant VACV derived from vaccination strain Praha expressed either the sTβRII (ectodomain) or chimeric protein fused to immunoglobulin Fc fragment (sTβRII-Fc-Jun) under control of two different promotors together with the immunogenic tumor associated antigen HPV16 E7 oncoprotein in a form of SigE7LAMP fusion molecule. The ability of soluble receptors to bind TGF-β in vitro was proved. Immunization of mice with double recombinant viruses and virus expressing Sig... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600155 Thu, 17 Mar 2011 02:30:03 +0100 4600155 http://www.medworm.com/index.php?rid=4600153&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391734%26dopt%3DAbstract In conclusion, cytoplasmic expression of CD40 is related to factors connected to better prognosis and suggest that CD40 may have potential as a new prognostic factor in breast cancer. Keywords: breast cancer, CD40 expression, luminal A subtype, Bcl-2. PMID: 21391734 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600153 Thu, 17 Mar 2011 02:30:03 +0100 4600153 http://www.medworm.com/index.php?rid=4600152&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391735%26dopt%3DAbstract Authors: Cvelbar M, Hocevar M, Vidmar G, Teugels E The aim of the study was to analyse the results of BRCA1/2 testing in a group of patients with double primary breast and ovarian cancer (DPBOC) in Slovenia. Additionally, the family history and the clinicopathologic characteristics of BRCA1/2 mutation positive and negative patients with DPBOC were analysed, comparing them to a group of untested patients with DPBOC. For these groups of patients, survival analysis was also performed. From the 52 patients who were invited to genetic counselling and testing, 20 responded positively (38% compliance). BRCA1/2 mutations were found in 60% (12/20): 45% BRCA1 and 15% BRCA2 (9 and 3 patients, respectively). There was significantly higher grade of ovarian cancer and significantly higher rate of ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600152 Thu, 17 Mar 2011 02:30:03 +0100 4600152 http://www.medworm.com/index.php?rid=4600151&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391736%26dopt%3DAbstract Authors: Lin BL, Xie DY, Xie SB, Xie JQ, Zhang XH, Zhang YF, Gao ZL The oncoprotein inhibitory member of the ASPP family (iASPP) is a key inhibitor of the p53 tumor suppressor and is upregulated in patients with acute leukemia and breast carcinoma. To investigate the effect of iASPP inhibition on the proliferation of hepatocellular carcinoma cells, a recombinant lentivirus vector expressing a small interfering RNA (siRNA) against iASPP gene expression was constructed and used to infect human hepatocellular carcinoma cells (HepG2 and Hep3B). The results showed that iASPP mRNA and protein levels were significantly down-regulated in both cells infected with the siRNA against iASPP. siRNA-mediated down-regulation of iASPP repressed tumor cell proliferation and colony formation in vitro ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600151 Thu, 17 Mar 2011 02:30:03 +0100 4600151 http://www.medworm.com/index.php?rid=4600150&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391737%26dopt%3DAbstract Authors: Jablonska E, Slodczyk B, Wawrusiewicz-Kurylonek N, Garley M, Dziemianczyk D, Kretowski A, Grabowska SZ In the present study the expression of tumor-promoting B cell-activating factor (BAFF), a member of the tumor necrosis factor superfamily (TNF) in neutrophils from oral cavity cancer patients, was examined by real-time PCR. For the purpose of comparison, the expression of BAFF protein was assessed in autologous peripheral blood mononuclear cells (PBMCs). An important question of this study has also been to explain the role of NF-κB in the induction of BAFF molecule.<br />The increased expression of BAFF at the mRNA and protein levels in neutrophils and mononuclear cells of patients before and after treatment were accompanied by the increased expression of phospho-IκB... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600150 Thu, 17 Mar 2011 02:30:03 +0100 4600150 http://www.medworm.com/index.php?rid=4600149&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391738%26dopt%3DAbstract Authors: Trivedi TI, Tankshali RA, Goswami JV, Shukla SN, Shah PM, Shah NG The purpose of the present study was to identify site-specific prognostic biomarkers in patients with oral squamous cell carcinoma (OSCC). For this purpose, Epidermal growth factor receptor (EGFR), Stat3, H-ras, c-myc, p53, cyclin D1, p16, Rb and Bcl-2 were localized immunohistochemically in buccal mucosa carcinoma (n=74) and tongue carcinoma (n=61) patients. Expression of markers was compared between buccal mucosa and tongue carcinoma and assessed for their prognostic value in site-specific manner. On comparison, only cyclin D1 showed significant difference in expression with higher accumulation in tongue tumors (r=+0.177, p=0.039). Moreover, univariate survival analysis showed that in buccal mucosa patients, l... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600149 Thu, 17 Mar 2011 02:30:03 +0100 4600149 http://www.medworm.com/index.php?rid=4600148&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391739%26dopt%3DAbstract Authors: Cichorek M Camptothecin and its analogues are used as S-phase specific antitumor drugs because of topoisomerase I inhibition providing cells to death by apoptosis. Our previous works documented that amelanotic hamster's melanoma is very sensitive to camptothecin. Because of the challenges in treating melanoma and S-phase specificity of camptothecin, we performed a study to search what melanoma cell cycle phases are susceptible to this substance.<br />Melanotic (Ma) and amelanotic (Ab) lines of Bomirski hamster's melanoma were used. Camptothecin cytotoxicity was determined by TUNEL method and cell cycle analysis was done by DNA staining with propidium iodide.<br />Camptothecin after short time killed amelanotic melanoma cells from S/G2/M phases but with extended t... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600148 Thu, 17 Mar 2011 02:30:03 +0100 4600148 http://www.medworm.com/index.php?rid=4600147&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391740%26dopt%3DAbstract Authors: Mytnik M, Stasko J The relationship between malignant tumors and blood coagulation disorders is generally well known. The authors studied blood coagulation in patients with colorectal cancer and evaluated some prothrombotic markers. <br />The authors analyzed by latex-aglutination method, ProC Global test, Asserachrom PAI-1 test and Enzygnost F 1+2 test the group of 137 patients with malignant tumor of colon and rectum, drew attention to the relationship between level of D-dimer, PAI-1, F 1+2, Protein C and the progress of malignant tumor, its localization, clinical stage, histopathology type, method of surgery considering the stapling use. <br />Very aggressive and advanced tumors have significantly higher level of D-dimer, plasminogen activator inhibitor I (PAI-1... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600147 Thu, 17 Mar 2011 02:30:03 +0100 4600147 http://www.medworm.com/index.php?rid=4600146&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391741%26dopt%3DAbstract Authors: Wang XH, Meng XW, Sun X, Liu BR, Han MZ, DU YJ, Song YY, Xu W In hepatocellular carcinoma (HCC), Wnt/β-catenin, Ras/MAPK and PI3K/AKT signaling pathways form a complex network and play important roles during HCC genesis and development. To study their relationship and the influence on cell growth, the siRNA directed against β-catenin was transfected into HCC HepG2 cells. β-catenin mRNA and protein levels were measured respectively at various times by RT-PCR and Western blot. Furthermore, HCC cell growth was measured by MTT assay. Finally, MAPK family and Akt1 protein levels were also measured by Western blot. After the transfection, β-catenin mRNA levels were markedly inhibited at 24 h and increased gradually at 48, 72 and 96 h; β-catenin protein levels decreased gradual... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600146 Thu, 17 Mar 2011 02:30:03 +0100 4600146 http://www.medworm.com/index.php?rid=4600145&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391742%26dopt%3DAbstract In conclusion, high FDG uptake measured by F-18 FDG PET/CT might have a prognostic value for overall survival and DFS in surgically resected early stage (stage I and II) NSCLC even after stratified by pathologic stages.  Keywords: F-18 FDG, Early stage, non-small cell lung cancer, overall survival, disease free survival. PMID: 21391742 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600145 Thu, 17 Mar 2011 02:30:03 +0100 4600145 http://www.medworm.com/index.php?rid=4600144&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391743%26dopt%3DAbstract In this study, we investigated the expression of TLR9 in breast cancer specimens and determined the association between its expression and the clinicopathological features observed. We found that TLR9 expression was significantly higher in patients with breast cancers displaying large tumor size (P = 0.040), lymph node metastasis (P Keywords: breast cancer, TLR9, lymph node metastasis. PMID: 21391743 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600144 Thu, 17 Mar 2011 02:30:03 +0100 4600144 http://www.medworm.com/index.php?rid=4600143&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21395367%26dopt%3DAbstract Authors: Voglova J, Muzik J, Faber E, Zackova D, Klamova H, Steinerova K, Michalovicova Z, Demitrovicova L, Cmunt E, Novakova L, Tothova E, Belohlavkova P, Mayer J, Indrak K Tyrosine kinase inhibitors (TKI) have completely changed the prognosis of patients with Ph+ chronic myeloid leukemia (CML). The occurrence of a second malignancy (SM) in CML patients successfully treated with TKI may significantly affect their prognosis.<br />In a retrospective study of 1,038 patients with CML treated at 10 centers in the Czech Republic and Slovakia between 2000 and 2009, SM was detected in 35 (3.37%) patients after TKI therapy was initiated. The median intervals from the diagnosis of CML and from the start of TKI therapy to the diagnosis of SM were 58 months (range 2 - 214) and 32 months (... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600143 Thu, 17 Mar 2011 02:30:03 +0100 4600143 http://www.medworm.com/index.php?rid=4600142&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21395368%26dopt%3DAbstract Authors: Prochazka V, Papajik T, Gazdova J, Divoka M, Rozmanova S, Faber E, Raida L, Kucerova L, Langova K, Jarosova M, Indrak K Antibody (rituximab) dependent cellular cytotoxicity is a key mechanism in killing CD20+ lymphoma cells. FcγRIIIA-158 V/F gene polymorphism results in expression of 3 variants of the FcγRIIIA receptor (FcγRIIIA) on cytotoxic lymphocytes with different receptor affinity. <br />We studied 102 patients with newly diagnosed FL to assess whether the FcγRIIIA genotype influences outcome in patients treated with risk-adapted immunochemotherapy. The median age was 52 years (31-84); 90% of the patients had advanced (III/IV) clinical stages. <br />The Follicular Lymphoma International Prognostic Index (FLIPI) scores were as follows: low 18.9%, intermed... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4600142 Thu, 17 Mar 2011 02:30:03 +0100 4600142 http://www.medworm.com/index.php?rid=4483125&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275457%26dopt%3DAbstract Authors: Gouveris H, Lehmann CG, Heinrich UR, Mann WJ, Brieger J The aim of this study was to investigate whether so far unknown chromosomal alterations in pleomorphic adenoma (PA) exist. To this end, tissue samples from 18 patients with parotid gland PA were studied by comparative genomic hybridization (CGH) using Phi-29-DNA-polymerase for DNA amplification. The most common aberrations were losses of chromosomal material of 19p (6/18), 9q, 16p, and 19q (in 3 out of 18 patients each). Additional losses were observed on 4p, 5q, and 17q (2 / 18 each). Gains involved chromosomes 2p, 4p, 6p, 17q, and 21q (2 / 18 each). Losses of 19p have been associated with inactivation of tumor-suppressor genes in carcinomas previously. As a result, pleomorphic adenomas show a considerable diversity of c... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483125 Wed, 16 Feb 2011 18:15:03 +0100 4483125 http://www.medworm.com/index.php?rid=4483124&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275458%26dopt%3DAbstract Authors: Jia L, Yi XF, Zhang ZB, Zhuang ZP, Li J, Chambers SK, Kong BH, Zheng W <p>The exact role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian epithelial carcinoma (OEC) development has not been yet characterized. This prompted us to identify particular proteins to better understand the underlying mechanism. Total proteins from ovarian epithelial tumor (OET) cells treated with gonadotropins were analyzed by proteomics. Western blot and immunohistochemistry were used to validate the target protein (prohibitin) and to detect its expression in human ovarian tissue of serous tumors. As the results, prohibitin was found to be significantly up-regulated by LH, with a maximum of 2.5-fold increase at the concentration of 200 mIU/mL. </p><p>Th... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483124 Wed, 16 Feb 2011 18:15:03 +0100 4483124 http://www.medworm.com/index.php?rid=4483123&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275459%26dopt%3DAbstract Authors: Guo Y, Guo W, Chen Z, Kuang G, Yang Z, Dong Z The canonical Wnt signalling pathway plays a key role during embryogenesis and pathogenesis of various types of tumors. Recently, several studies have shown that the promoter hypermethylation of Wnt-antagonist genes, including sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wif-1 and Dkk-3, have been certified to contribute to the tumorigenesis of several cancers. <br />The aim of this study was to investigate the promoter methylation of Wnt-antagonist genes in gastric cardia adenocarcinoma (GCA) and corresponding adjacent non-cancerous tissues, and to establish the possible relationship between DNA methylation status and the pathogenesis of GCA.<br />MSP, RT-PCR methods were applied respectively to examine the CpG methylation of the W... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483123 Wed, 16 Feb 2011 18:15:03 +0100 4483123 http://www.medworm.com/index.php?rid=4483122&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275460%26dopt%3DAbstract Authors: Ghoneum M, Gollapudi S The present study was carried out to investigate the synergistic apoptotic potential of arabinoxylan rice bran (MGN-3/Biobran) and curcumin (turmeric) on human multiple myeloma (MM) cell line U266 , in vitro. U266 cells were cultured with MGN-3 (50 or 100μg/ml) and curcumin (2.5-10μM) for 3 days. The effects of MGN-3 and curcumin on the growth and survival of the U266 cells were determined by trypan blue, MTT assay, flow cytometry analysis of cancer cell cycle, and apoptosis. Expression of proapoptotic Bax, and antiapoptotic Bcl2 was determined by Western blot analysis. Treatment with MGN-3 alone or curcumin alone caused a dose-dependent inhibition in the proliferation of U266 cells. However, a synergistic effect was noticed post-treatment with both ag... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483122 Wed, 16 Feb 2011 18:15:03 +0100 4483122 http://www.medworm.com/index.php?rid=4483121&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275461%26dopt%3DAbstract Authors: Hlavna M, Kohut L, Lipkova J, Bienertova-Vasku J, Dostalova Z, Chovanec J, Vasku A Cancer of endometrium (CAE) is the most common gynecologic malignancy in industrialized nations. Increased resistin levels, an adipocytokine produced by adipose tissue and macrophages, have been considered as a risk factor in gastric, colon and breast cancer, recently. No studies associating resistin levels with endometrial cancer have been done so far. The purpose of this case-control study was to determine the relationship between serum circulating resistin levels and resistin gene -420C>G (rs3219175) variant in endometrial cancer patients. 37 Caucasian female patients and 39 healthy controls were enrolled in this study. Difference in resistin levels between age and BMI matched patients gro... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483121 Wed, 16 Feb 2011 18:15:03 +0100 4483121 http://www.medworm.com/index.php?rid=4483120&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275462%26dopt%3DAbstract Authors: Jagielska B, Symonides M, Stachurska E, Kawecki A, Kraszewska E The aim of the study was to analyse coagulation disorders in patients with locally advanced cancer of the head and neck (CHN)and with no other clinical cause for coagulation disorders treated with radiation therapy alone or concurrent chemoradiotherapy. We also assessed the duration of disorders in the course of therapy. <br />The analysed group consisted of 33 patients with locally advanced CHN documented as stage T3 or T4 acc. to the TNM classification. Coagulology tests (activated partial thromboplastic time /APTT/, prothrombin time, fibrinogen concentration, euglobulin lysis time, C - reactive protein and anti-thrombin III concentration, d-dimer level, PAI-1, plasminogen level and plasmin-anti-plasmin as... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483120 Wed, 16 Feb 2011 18:15:03 +0100 4483120 http://www.medworm.com/index.php?rid=4483119&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275463%26dopt%3DAbstract This study aimed to evaluate the role of 18F-FDG PET/CT in CUP. <br />Fifty-one patients (19 women, 32 men) with metastasis confirmed by histopathology from an unknown primary tumor were included in this study. Patients received 370 MBq of 18F-FDG intravenously, and PET/CT was performed at 60 minutes after injection. <br />Primary tumor sites were detected in 5 of 51 patients (9.6%): in 2 patients with carcinoma of the lung, in 1 patient with carcinoma of the gallbladder, in 1 patient with carcinoma of the esophagus, and in 1 patient with carcinoma of the stomach. No primary tumor was discovered in the remaining 46 patients (90.4%) during the follow-up. The sensitivity, specificity, and accuracy of 18F-FDG PET/CT were 100%, 80.4%, and 82.4%. The positive and negative predictive... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483119 Wed, 16 Feb 2011 18:15:03 +0100 4483119 http://www.medworm.com/index.php?rid=4483118&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275464%26dopt%3DAbstract In conclusion, our results suggest that measurement of ZAP-70 expression in CLL by MFI using T-cells/CLL cells ratio might be the optimal method for accurate prediction of clinical course. Combined analysis of ZAP-70 with CD38 or IgVH mutation status further refined individual patient´s prognosis. Keywords: chronic lymphocytic leukemia, ZAP-70 expression, mean fluorescence intensity, isotype control, prognosis. PMID: 21275464 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483118 Wed, 16 Feb 2011 18:15:03 +0100 4483118 http://www.medworm.com/index.php?rid=4483117&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275465%26dopt%3DAbstract Authors: Aribaş BK, Arda K, Aktaş E, Ciledağ N, Yakut F, Sahin G, Coşar Y, Doğan K, Kaya G Our objective was to examine the variables affecting diagnostic yield and complications in percutaneous ultrasonography-guided needle biopsies of solid renal masses. Percutaneous ultrasonography-guided needle biopsy of solid renal masses was performed in 172 patients with either large size (18G) cutting needles or small size (20G) aspiration needles. Retrospectively, 120 patients with diagnosis by percutaneous biopsy and follow-up data were included in this series. Age, gender, side, locations in kidneys, necrosis, calcification, maximum size, needle groups due to needle size and type (either 18G cutting needles or 20G aspiration needles), and needle pass were selected as variables. Their ro... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483117 Wed, 16 Feb 2011 18:15:03 +0100 4483117 http://www.medworm.com/index.php?rid=4483116&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275466%26dopt%3DAbstract Conclusion: the increased expression of JMJD1A might be associated with the progression of kidney cancer. Keywords: renal cell carcinoma, histone demethylase, JMJD1A, hypoxia-inducible factor, iron. PMID: 21275466 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483116 Wed, 16 Feb 2011 18:15:03 +0100 4483116 http://www.medworm.com/index.php?rid=4483115&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275467%26dopt%3DAbstract Authors: Wang XH, Liu BR, Qu B, Xing H, Gao SL, Yin JM, Wang XF, Cheng YQ The genesis and development of hepatocellular carcinoma (HCC) is related to the abnormity of signaling pathway, telomerase, cell cycle, apoptosis, angiogenesis, and others, in which STAT3 signaling pathway plays a key role. The HCC cell line HepG2 was transfected with small interfering RNA (siRNA) directed against STAT3. After 72 h, cell growth and cycle were analysed by MTT and Flow cytometry. Then, the protein was extracted and the protein expression of STAT3, Smad3, p44/42, TERT, caspase-3, XIAP, Grp-78, HSP-27, MMP-2, MMP-9, VEGF-A, cyclin A, and cyclin E was detected by Western blot. After the transfection, HCC cell growth was inhibited during the 24-72 h time period and the cell cycle was arrested in G0/G1.... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483115 Wed, 16 Feb 2011 18:15:03 +0100 4483115 http://www.medworm.com/index.php?rid=4483114&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21275468%26dopt%3DAbstract Authors: Vrdoljak E, Boban M, Omrcen T, Hrepic D, Fridl-Vidas V, Boskovic L Optimal first-line chemotherapy for metastatic breast cancer (MBC) is challenging, particularly in patients previously treated with (neo) adjuvant anthracyclines/taxanes. Based on preclinical synergy with mitomycin C (MMC) and capecitabine in human tumor xenografts, we conducted a phase II study of first-line capecitabine and MMC in MBC. Patients received 3-weekly chemotherapy comprising MMC 8 mg/m2 day 1 and capecitabine 1000 mg/m2 twice daily, days 1-14. Combination chemotherapy was administered for a maximum six cycles, single-agent capecitabine could be continued until progressive disease or unacceptable toxicity. Thirty patients were included, objective response rate was 65.5%. After a median follow-up... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483114 Wed, 16 Feb 2011 18:15:03 +0100 4483114 http://www.medworm.com/index.php?rid=4483113&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21288054%26dopt%3DAbstract Authors: Ondrusova M, Ondrus D, Muzik J, Hunakova L, Hes O, Karabinos J, Friedova L Incidence rates of kidney cancer vary in global standards more than tenfold, the highest worldwide rates are repeatedly registered in the Czech Republic (hereafter CR) and in other geographically neighbouring countries in Central Europe, including the Slovak Republic (hereafter SR). The aim of the study was to analyse the changes in time trends of incidence and mortality from kidney cancer in two geographically close countries (CR and SR) and to compare detected differences with the worldwide data. In spite of high rates of incidence and its global growth in analyses in 1980 - 2005, the character of its progress in time was changed in both countries. While in 1980 - 1994 the incidence of kidney cancer i... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4483113 Wed, 16 Feb 2011 18:15:03 +0100 4483113 http://www.medworm.com/index.php?rid=4171136&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067259%26dopt%3DAbstract Authors: Roziakova L, Bojtarova E, Mistrik M, Mladosievicova B Hematopoietic stem cell transplantation (HSCT) offers patients with malignant and nonmalignant diseases the oportunity to pursue life-prolonging therapy. The number of survivors after successful HSCT is continually increasing. However, HSCT can induce tissue and organ damage that occurs not only "on treatment" , but long after completing therapy. Secondary malignancies belong to serious late complications after HSCT. A significant association of certain risk factors with increased likelihood of secondary malignancies after HSCT has been published over the last ten years. Better knowledge of pathogenesis of these complications, their early identification and treatment may contribute to better health outcomes of allogeneic a... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171136 Tue, 16 Nov 2010 19:02:02 +0100 4171136 http://www.medworm.com/index.php?rid=4171135&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067260%26dopt%3DAbstract Authors: Yu L, Wang L, Chen S Olfactomedin 4 (OLFM4) is a member of olfactomedin domain-containing protein family. Human OLFM4 is preferentially expressed in the gastrointestinal tract (stomach, small intestine and colon), prostate, and bone marrow. Recent studies demonstrate that OLFM4 is involved in the establishment and/or development of some types of malignancies, especially in gastrointestinal cancers. Induction of OLFM4 in cancer cells has a novel antiapoptotic action and promotes proliferation of cancer cells. OLFM4 regulates cell cycle and promotes S phase transition in proliferation of cancer cells. In addition, OLFM4 is associated with cancer adhesion and metastasis. In this minireview, we mainly focus on the OLFM4 expression and its biological significances in tumor diffe... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171135 Tue, 16 Nov 2010 19:01:48 +0100 4171135 http://www.medworm.com/index.php?rid=4171134&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067261%26dopt%3DAbstract Authors: Slamenova D, Horvathova E, Chalupa I, Wsolova L, Navarova J <p>Carvacrol belongs to frequently occurring phenolic components of essential oils (EOs) and it is present in many kinds of plants. Biological effect of this phenol derivative on human beings is however not sufficiently known. The present study was undertaken to evaluate the level of VL+MB-induced oxidative DNA lesions in hepatocytes and testicular cells (freshly isolated from control or carvacrol-watered rats) by the modified single cell gel electrophoresis (SCGE). The results showed that carvacrol significantly reduced the level of VL+MB-induced oxidized bases (EndoIII- and Fpg-sensitive sites) only in hepatocytes but not in testicular cells. Chromosomal aberration assay of primary hepatocytes, isolated from c... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171134 Tue, 16 Nov 2010 19:01:34 +0100 4171134 http://www.medworm.com/index.php?rid=4171133&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067262%26dopt%3DAbstract In this study, we used the property that the macrophages in omental milky spots are cytotoxic against tumor cells and so able to screen and collect cancer stem cells. Our findings suggest that macrophages in omental milky spots have not only cytotoxic properties against tumor cells but also provide a microenvironment within milky spots in which cancer stem cells are capable to survive and grow into micrometastasis. Omental milky spot become a cancer stem cell niche in this situation. Further we studied the omental milky spots for screening gastric cancer cells (OMSS-GCCs) and found that omental milky spot enriched the volume of gastric cancer stem cells. Tumors were consistently generated after an injection of 1×103 OMSS-GCCs. OMSS-GCCs high express CD133 and low express CD324. Omental ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171133 Tue, 16 Nov 2010 19:01:25 +0100 4171133 http://www.medworm.com/index.php?rid=4171132&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067263%26dopt%3DAbstract Authors: Koo JS, Kim SH We investigated EGFR and HER-2 status in brain metastatic non-small cell lung cancer (NSCLC) and compared them to EGFR and HER-2 status of primary NSCLC. Evaluated were 66 cases of brain metastatic NSCLC, including 20 cases of corresponding primary NSCLC. HER-2 status was investigated by immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH), and EGFR status was evaluated by IHC. HER-2 overexpression and/or amplification was/were observed in three cases (4.5 %) of 66 cases of brain metastatic NSCLC, and 23 cases (34.8%) demonstrated EGFR overexpression. Among 20 cases of primary and corresponding metastatic NSCLC, one case showed HER-2 overexpression and amplification in both primary and metastatic tumor. On the other hand, EGFR overexpression... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171132 Tue, 16 Nov 2010 19:01:19 +0100 4171132 http://www.medworm.com/index.php?rid=4171131&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067264%26dopt%3DAbstract Authors: Elknerova K, Myslivcova D, Lacinova Z, Marinov I, Uherkova L, Stöckbauer P Histone deacetylase inhibitors (HDACi) are emerging new class of anticancer agents that act by inhibiting cell growth, inducing cell cycle arrest and apoptosis of various cancer cells. However, in some conditions, apoptosis can be blocked and non apoptotic cell death and irreversible growth arrest, namely senescence, can be activated as potential tumor-suppressor mechanism. Here we evaluated the dosage effects of HDAC inhibitors suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) in a series of human leukaemia cell lines. We investigated, what concentration of SAHA and VPA can optimally induce apoptosis, growth inhibition or stress-induced premature senescence. We have found that SAHA inhib... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171131 Tue, 16 Nov 2010 19:01:13 +0100 4171131 http://www.medworm.com/index.php?rid=4171130&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067265%26dopt%3DAbstract Authors: Sawicka-Powierza J, Jablonska E, Kloczko J, Piszcz J, Garley M, Ratajczk-Wrona W It was demonstrated that TNF superfamily proteins may affect significantly the time of leukemic cells' survival in the course of B-cell chronic lymphocytic leukemia (B-CLL).<br />The aim of our study was to evaluate the expression and release of BAFF (B-cell activating factor), APRIL (a proliferation-inducing ligand) and TRAIL (TNF-related apoptosis inducing ligand) molecules belonging to the cytokines of the superfamily of the tumor necrosis factor (TNF) by neutrophils (PMNs) and, for comparison, B cells isolated from the blood of patients with B-CLL vs. their concentration in the blood serum. 40 patients suffering from B-CLL and a control group of 15 healthy subjects were included in the... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171130 Tue, 16 Nov 2010 19:01:03 +0100 4171130 http://www.medworm.com/index.php?rid=4171129&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067266%26dopt%3DAbstract The objective of the study was to determine the predicting factors in malignant diagnosis in ultrasonography guided fine-needle aspiration biopsy of cervical lymph nodes. Design is retrospective follow-up study. Ultrasonography guided fine-needle aspiration biopsies of cervical lymph nodes were performed in 290 patients. The mean age was 45.5±14.4 years (range; 15-85). 207 (71.4%) and 83 (28.6%) were women and men, respectively. Cytopathologist was not present in any biopsy procedure. Factors in predicting malignancy were age, gender, presence of primary malignancy, localization (Level 1-6), hypoechogenicity with loss of echogenic hilum, microcalcification, cystic feature, minimum and maximum sizes, and index value (minimum size/maximum size). Factors were analyzed by univariate and multi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171129 Tue, 16 Nov 2010 19:00:55 +0100 4171129 http://www.medworm.com/index.php?rid=4171128&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067267%26dopt%3DAbstract Authors: Krechler T, Zeman M, Vecka M, Macasek J, Jachymova M, Zima T, Zak A The aim of this study was to analyze the relationship of serum leptin as well as adiponectin and the manifestation of pancreatic cancer (PC).  Serum leptin, adiponectin, glucose homeostasis and insulin resistance (expressed as HOMA-IR) were investigated in 64 patients with newly diagnosed PC and compared with 64 healthy controls (CON group) and 75 patients with type 2 diabetes (DM2).<br />Seventy percent of newly diagnosed PC patients had DM2. The levels of leptin were lower, whilst adiponectin/leptin ratio was higher in PC patients (both with and without DM2), in comparison with CON and DM2 groups (P < 0.001) independently of age, BMI and waist circumference.<br />Newly diagnosed PC is charact... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171128 Tue, 16 Nov 2010 19:00:46 +0100 4171128 http://www.medworm.com/index.php?rid=4171127&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067268%26dopt%3DAbstract In this study, we constructed a model of migration-activated glioma cells by using the migration-stimulating substrate, a kind of ECM, laminin in vitro. Our results present evidence that laminin drives glioma cell migration without altering cell proliferation. Further, actively migrating cells which expressioned high phosphorylation of p27 at Ser10, and induced its cytoplasmic localization. In this process, Jab1 and CRM1 were also involved. Thus phosphorylation of p27 at Ser10 is necessary for both cytoplasmic localization and induction of cell migration. These observations solidified a genetic role of p27 in cell migration and this was independent of cyclin/CDK inhibition. Eventually, we transiently transfected p27S10A into T98G glioma cells, found that overexpression of p27S10A inhibi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171127 Tue, 16 Nov 2010 19:00:38 +0100 4171127 http://www.medworm.com/index.php?rid=4171126&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067269%26dopt%3DAbstract Authors: Li M, Zhang Q, Liu L, Liu Z, Zhou L, Wang Z, Yue S, Xiong H, Feng L, Lu S Mutations of epithelial growth factor receptor (EGFR) in exon 19 and 21 are both believed to be associated with carcinogenesis, sensitivity to tyrosine kinase drugs and with the prognosis of non-small cell lung cancers (NSCLCs). However, their exact clinical significance remains disputable. We detected the mutations of 157 NSCLCs from mainland China with high resolution melting analysis and identified exon mutations with DNA sequencing. Of the 157 cases examined, 57 displayed EGFR mutations which included 22 in exon 19 and 35 in exon 21. Current research has shown that EGFR mutations are more commonly associated with the female population and East Asians, with additional significance being adenocarcinoma... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171126 Tue, 16 Nov 2010 19:00:29 +0100 4171126 http://www.medworm.com/index.php?rid=4171125&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067270%26dopt%3DAbstract Authors: Giertlova M, Hajikova M, Vaskova J, Kafkova A, Stecova N, Mirossay L, Karabinos A, Tothova E, Sarissky M We evaluated the prognostic impact of chromosomal abnormalities as detected by interphase fluorescence in situ hybridization (iFISH) in 86 chronic lymphocytic leukemia (CLL) patients. Overall, 39 of 86 (45%) patients displayed one (35%) or more (10%) chromosomal abnormalities, del13q (31%) being more frequently detected than trisomy 12 (19%) followed by del11q (17%), del17p (6%) and del6q (5%). Significant differences in the treatment free intervals (TFIs) were observed among individual cytogenetic subgroups (p=0.027) with the shortest mean TFIs in subgroups with del17p, del11q and trisomy 12 (10, 12 and 14 months, respectively) as compared to subgroups with normal cytogene... Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171125 Tue, 16 Nov 2010 19:00:18 +0100 4171125 http://www.medworm.com/index.php?rid=4171124&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21067271%26dopt%3DAbstract In conclusion, hypermethylation leads to silencing of the Syk gene in human lung carcinoma cell lines. Methylation of the Syk promoter and loss of Syk expression in lung cancer cell lines are independent biomarkers. Syk may be a potential tumor suppressor in human lung cancer. Keywords: 5-aza-2'-deoxycytidine, lung cancer, MSP, promoter methylation, Syk, tumor suppressor gene. PMID: 21067271 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=4171124 Tue, 16 Nov 2010 19:00:09 +0100 4171124 http://www.medworm.com/index.php?rid=3982353&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845986%26dopt%3DAbstract Authors: Smetana K, Karban J, Trneny M The present study was undertaken to provide more information on nucleoli in lymphocytes of B - chronic lymphocytic leukemia. The computer assisted nucleolar and cytoplasmic RNA image densitometry, reflecting the nucleolar and cytoplasmic RNA concentration at the single cell level, demonstrated a remarkable stability during the differentiation and maturation of B- lymphocytes. In contrast, as it was expected, the nucleolar diameter during the lymphocytic development markedly decreased. Thus the nucleolar RNA content of leukemic B-lymphocytes was apparently related to the nucleolar size. In both immature and mature lymphocytes, the cytostatic treatment increased the incidence of micronucleoli, which represent the "inactive" type of nucleoli. H... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982353 Mon, 20 Sep 2010 01:57:07 +0100 3982353 http://www.medworm.com/index.php?rid=3982352&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845987%26dopt%3DAbstract In this study, we chose osteosarcoma cell line MG-63, which highly expressed survivin, to observe the effects of antisense oligonucleotide targeting survivin on the apoptosis induction and proliferation inhibition. It was shown in our results that the apoptosis rate and the proliferation inhibition rate increased significantly in survivin-positive cells MG-63 by using MTT and flow cytometry methods. We found that the growth inhibition rate and apoptosis rate were changed in a dose-dependent way. When the concentration of antisurvivin oligonucleotide was 600 nM, the effects reached the peak. RT-PCR and western-blot methods were used to detect the mRNA and protein expression of survivin in MG-63. We observed that the mRNA and protein expression of survivin reduced after transfected with a... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982352 Mon, 20 Sep 2010 01:57:07 +0100 3982352 http://www.medworm.com/index.php?rid=3982351&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845988%26dopt%3DAbstract This study aimed at comparing the properties of plasma cell-free DNA with the biopsy's DNA in order to evaluate the clinical significance of IgH and TCRγ gene rearrangement in plasma cell-free DNA from patients with non-Hodgkin's Lymphoma. A total of 360 samples were studied. IgH (FR3A/VLJH) and TCRγ (TVG/TJX) were amplified by PCR. Results of plasma cell-free DNA were compared with biopsy's DNA and mononuclear cellular DNA respectively. Plasma cell-free DNA were successfully extracted from 288 cases of newly diagnosed, refractory and relapsed NHL in total 360 patients (80%).But nothing was found in the other 72 remittent patients. The positive percentage of IgH rearrangement in patients with B-NHL was 81% in plasma cell-free DNA and 77% in biopsy's DNA (P>0.05). As to the rati... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982351 Mon, 20 Sep 2010 01:57:07 +0100 3982351 http://www.medworm.com/index.php?rid=3982350&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845989%26dopt%3DAbstract In this study, CYP1A1 (Ile462Val), CYP1B1(Asn453Ser), GST M1, GSTP1 exon 5 (Ile105Val) and exon 6(Ala114Val) and GSTT1 polymorphisms were determined in 138 patients with advanced NSCLC to evaluate their role in survival. Of the studied CYP and GST polymorphisms only GSTP1 exon 6 variant significantly altered (improved) the survival compared to wild type (p=0.036) with median survival of 22.2 months and 16.1 months, respectively. Multivariate analysis also revealed a significant reduction of adjusted hazard ratio of death associated only with the GSTP1 exon 6 variant genotype of 0.45 (95% confidence interval (95% CI), 0.23-0.89, p=0.022). These results show that the GSTP1 exon 6 variant genotype is associated with improved survival in the patients with advanced NSCLC. Keyword... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982350 Mon, 20 Sep 2010 01:57:07 +0100 3982350 http://www.medworm.com/index.php?rid=3982349&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845990%26dopt%3DAbstract Authors: Chen W, Ke Z, Shi H, Yang S, Wang L The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of AEG-1 in 8 cases of RCC tissue compared with the paired normal tissue from the same patient. The expression level of AEG-1 was also increased in four RCC cell lines in contrast with normal tubular epithelial human kidney cells 2 (HK-2) at both mRNA and protein levels. Furthermore, immunohistochemistry analysis showed highly expressed AEG-1 in 96 of 102 (94.1%) cases of paraffin-embedded archival RCC tissue. Statistical analysis showed a ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982349 Mon, 20 Sep 2010 01:57:07 +0100 3982349 http://www.medworm.com/index.php?rid=3982348&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845991%26dopt%3DAbstract We examined the impact on patients' survival of several phenotypic aberrancies detected by a small 4-color panel of monoclonal antibodies (MoAbs). Diagnosis of the patients (54) was made by WHO criteria using peripheral blood counts, bone marrow (BM) morphology and karyotype. Flow cytometry was performed at diagnosis, and features obtained were compared to normal BM (24). We could detect 16 alterations: 4 in granulocytic precursors, 4 in monocytes, 6 in CD34+ cells, beside changes in plasmacytoid dendritic cells and basophil precursors. The total number of changes in RAEB was higher (median 8) than in cases with of abnormalities) were independent risk factors for a shorter survival. Our panel was sufficient to confirm the diagnosis of MDS and permitted to detect independent ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982348 Mon, 20 Sep 2010 01:57:07 +0100 3982348 http://www.medworm.com/index.php?rid=3982347&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845992%26dopt%3DAbstract Authors: Pietrowska M, Marczak L, Polanska J, Nowicka E, Behrent K, Tarnawski R, Stobiecki M, Polanski A, Widlak P Mass spectrometry-based analysis of the serum proteome allows identifying multi-peptide patterns/signatures specific for blood of cancer patients, thus having high potential value for cancer diagnostics. However, because of problems with optimization and standardization of experimental and computational design, none of identified proteome patterns/signatures was approved for diagnostics in clinical practice as yet. Here we compared two methods of serum sample preparation for mass spectrometry-based proteome pattern analysis aimed to identify biomarkers that could be used in early detection of breast cancer patients. Blood samples were collected in a group of 92 patients... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982347 Mon, 20 Sep 2010 01:57:07 +0100 3982347 http://www.medworm.com/index.php?rid=3982346&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845993%26dopt%3DAbstract Authors: Zhang XL, Peng CJ, Peng J, Jiang LY, Ning XM, Zheng JH Wnt7a is a secreted glycoprotein that regulates normal cellular proliferation and differentiation as well as tumorigenesis and progression. The aim of this study was to investigate the expression and prognostic significance of Wnt7a in ovarian carcinoma. Wnt7a expression was immunohistochemically examined in normal ovaries (n=15), benign tumors (n=50) and ovarian carcinomas (n=78). The correlation of Wnt7a expression with clinicopathological parameters and survival was evaluated. Wnt7a expression was higher in ovarian carcinomas compared to normal ovaries and benign tumors (p<0.001 and p=0.001, respectively). Wnt7a positive expression was significantly correlated with serous subtype (p<0.001), elder age (p=0.017), ad... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982346 Mon, 20 Sep 2010 01:57:07 +0100 3982346 http://www.medworm.com/index.php?rid=3982345&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845994%26dopt%3DAbstract Authors: Kolenova A, Hikkel I, Ilencikova D, Hikkelova M, Sejnova D, Kaiserova E, Cizmar A, Puskacova J, Bubanska E, Oravkinova I, Gencik M Acute lymphoblastic leukemia is the most common form of cancer in children. The 10-year event-free survival ranged from 77 to 85% after having achieved complete remission rates of 93% or higher. The main cause of treatment failure is relapse arising from outgrowth of residual leukemic cells that are refractory to therapy. An intense effort has been made to develop methods to determine the degree of minimal residual leukemia cells present in patients considered to be in morphological remission. Because of the strong correlation between minimal residual disease (MRD) levels and risk of relapse, monitoring of MRD provides unique information regarding ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982345 Mon, 20 Sep 2010 01:57:07 +0100 3982345 http://www.medworm.com/index.php?rid=3982344&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845995%26dopt%3DAbstract Authors: Z CZ, Luo C, Yang Z, Wang L <p>Although the expression of heparanase is associated with invasion and metastasis of various human cancers, the effects of heparanase on human osteosarcoma have not been evaluated. We showed that down-regulating the expression of heparanase significantly reduced proliferation and invasion of human U-2OS osteosarcoma cells. Furthermore, heparanase silencing by short-hairpin RNA (shRNA) was associated with decreased hypoxia-inducible factor-1α (HIF-1α) level, implying that heparanase was associated with the expression of HIF-1α. This result was confirmed by immunohistochemistry analysis. In osteosarcoma tissues, immunohistochemical results revealed that heparanase expression had a close correlation with that of HIF-1α and they h... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982344 Mon, 20 Sep 2010 01:57:07 +0100 3982344 http://www.medworm.com/index.php?rid=3982343&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845996%26dopt%3DAbstract We examined survivin expression in 13 cases of normal breast tissue, 38 cases of fibroadenomas and 80 cases of breast carcinomas by immunohistochemical staining using anti-survivin antibody (DAKO, Clone 12C4). In each section, the intensity of staining, percentage of labeled cells, and the subcellular location of survivin antigen were assessed. Survivin was detected in 4/13 cases of normal breast tissue (30.7%), in 28/38 cases of fibroadenomas (73.7%), and in 67/80 cases of carcinomas (83.8%). Normal breast tissue showed cytoplasmic positivity only. In fibroadenomas, 19 cases (50.0%) revealed cytoplasmic reaction, and in 9 cases (23.7%), small foci of cells with combined nuclear and cytoplasmic location were identified. In carcinomas, cytoplasmic staining was found in 12/80 cases (15.0%... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982343 Mon, 20 Sep 2010 01:57:07 +0100 3982343 http://www.medworm.com/index.php?rid=3982342&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845997%26dopt%3DAbstract Authors: Szotkowski T, Muzik J, Voglova J, Koza V, Maaloufova J, Kozak T, Jarosova M, Michalova K, Zak P, Steinerova K, Vydra J, Lanska M, Katrincsakova B, Sicova K, Pavlik T, Dusek L, Indrak K Acute myeloid leukemia (AML) is a severe condition with a high mortality. When making decisions about the optimal tailor-made therapy, numerous prognostic factors are considered. The study represents a detailed analysis of the role of these factors and treatment outcomes based on a long-term follow-up of patients treated in 5 hematology intensive care centers in the Czech Republic.<br />The studied group comprised 1,188 patients with de novo AML and 328 patients with secondary AML. The latter were significantly older, had more unfavorable cytogenetic changes and less frequently... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982342 Mon, 20 Sep 2010 01:57:07 +0100 3982342 http://www.medworm.com/index.php?rid=3982341&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845998%26dopt%3DAbstract Authors: Rotondo F, Sharma S, Scheithauer BW, Horvath E, Syro LV, Cusimano M, Nassiri F, Yousef GM, Kovacs K Vascularization is a prerequisite of tumor growth, invasion and metastasis. In the present work, microvessel density was assessed by quantitating using two different endothelial cell biomarkers, endoglin (CD-105) and CD-34. Fifty endocrinologically active and 36 clinically nonfunctioning pituitary adenomas, all surgically resected, as well as 10 autopsy-derived normal adenohypophyses were investigated by immunohistochemistry. The results showed that in every pituitary adenoma type endoglin, an assumed biomarker of proliferating endothelial cells, immunostained fewer vessels than CD-34 which revealed immunopositivity in all capillaries. Differences in endoglin versus CD-34 imm... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982341 Mon, 20 Sep 2010 01:57:07 +0100 3982341 http://www.medworm.com/index.php?rid=3982340&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20845999%26dopt%3DAbstract Authors: Liu B, Lu Z, Wang P, Basang Z, Rao X Recently oncogenetic human papilloma virus(HPV) infection has been suggested to promote laryngeal squamous cell carcinoma(LSCC). To determine the prevalence and genotypes of HPV infection in laryngeal cancer specimens, 84 specimens from pathologically confirmed LSCC patients were studied for the presence of viral DNA and possible virus integration into the cellular genome. HPV genotyping was assayed prior to the integration analysis by using two PCR-based assays, including HPV-16 and-18 E7 type-specific and L1 general primers (GP5+/GP6+). Additionally, a quantitative real-time PCR (qRT-PCR) was used to examine the physical status of HPV-16 or-18 in HPV positive LSCC. Using HPV L1 general primer amplification, HPV DNA was detected in 23 (... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3982340 Mon, 20 Sep 2010 01:57:07 +0100 3982340 http://www.medworm.com/index.php?rid=3695897&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568891%26dopt%3DAbstract Authors: Novotny L, Rauko P, Kombian SB, Edafiogho IO It is generally accepted that selenium (Se) plays an important role in maintaining equilibrium of a healthy organism. It also participates in processes related to carcinogenesis such as inhibition of tumor formation and regression. Scientific data accumulated so far using experimental animal models and from clinical studies devoted to investigating the effects of Se confirm strong relationship or correlation between Se supplementation and tumor frequency of prostate, lungs, liver and colon. However, details of mechanisms of action of Se in modulation of carcinogenesis and cancer prevention are not yet fully elucidated. It is not clear yet whether Se deficiency itself is a cancer risk factor or whether it helps an already present can... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695897 Fri, 25 Jun 2010 09:48:03 +0100 3695897 http://www.medworm.com/index.php?rid=3695896&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568892%26dopt%3DAbstract This study evaluates also recent knowledge of immunophenotype of leukemic stem cells and their more differentiated progeny committed to myeloid lineage - acute myeloid leukemia blast cells with regard to their phenotypic similarity to normal stem and granulocyte committed progenitor cells. Improved knowledge of normal stem and progenitor cells phenotypes, identifying new leukemia-specific markers, searching for aberrant marker expression and evaluation of aberrant intensity or combination of various marker expressions is important for distinguishing normal cells from their malignant counterpart in view of the diagnostics of leukemias or follow-up of minimal residual disease. <br /> Keywords: Immunophenotype, CD markers, hematopoietic stem cells, lineage-restricted progenitors, leukem... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695896 Fri, 25 Jun 2010 09:48:03 +0100 3695896 http://www.medworm.com/index.php?rid=3695895&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568893%26dopt%3DAbstract Authors: Cipak L, Jantova S The nuclear poly(ADP-ribose) polymerase-1 (PARP-1) represents an important novel target in cancer therapy. The enzyme is essential for single strand DNA breaks repair via base excision repair pathway. Inhibition of PARP-1 exerts "synthetic lethality" effect towards the tumors with defects in DNA repair by homologous recombination, specifically the tumors with mutations in the breast cancer associated BRCA1 and BRCA2 genes. Recent clinical data confirmed the early in vitro studies and suggest that PARP-1 inhibitors could be used not only as chemosensitizers but as well as single agents to selective kill tumors with defective DNA repair by homologous recombination. Such concept of "synthetic lethality" for tumors which have lost one DNA repair pathway by targe... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695895 Fri, 25 Jun 2010 09:48:03 +0100 3695895 http://www.medworm.com/index.php?rid=3695894&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568894%26dopt%3DAbstract In conclusion, the inhibitory effect of NaB on AR gene expression seems to be specific and unique for prostate cancer AR-positive cell lines and corresponds with its ability to stimulate AR-SMRT complex formation. We suggest that AR and SMRT/N-CoR corepressors may form a stable complex in vitro and NaB may facilitate the interaction between AR nuclear steroid receptor and SMRT corepressor prote<br /> Keywords: androgen receptor (AR), AR corepressor, HDAC inhibitor, histone deacetylases, chromatin immunoprecipitation (ChIP), sodium butyrate (NaB). PMID: 20568894 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695894 Fri, 25 Jun 2010 09:48:03 +0100 3695894 http://www.medworm.com/index.php?rid=3695893&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568895%26dopt%3DAbstract Authors: Soucek P, Susova S, Mohelnikova-Duchonova B, Gromadzinska J, Moraviec-Sztandera A, Vodicka P, Vodickova L The question of susceptibility to squamous cell carcinoma of head and neck (SCCHN) in the environmental context was addressed by analysis of functional polymorphisms in enzymes metabolizing smoke constituents and/or alcohol (CYP2A13, CYP1B1, EPHX1, NQO1, GSTM1, GSTP1, GSTT1, ADH1B and ADH1C). Case-control study of 122 age- and sex-matched pairs of subjects was performed using so far unexplored Central European Slavic population with high level of tobacco and alcohol abuse. <br />Age-, gender-, smoking- and alcohol-adjusted logistic regression failed to demonstrate any significant association of the analyzed polymorphisms with the SCCHN risk. When interactions between... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695893 Fri, 25 Jun 2010 09:48:03 +0100 3695893 http://www.medworm.com/index.php?rid=3695892&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568896%26dopt%3DAbstract In conclusion, the results of this study indicate that probiotic microorganisms and bioactive compounds could exert a preventive effect on colon carcinogenesis induced by DMH.<br /> Keywords: probiotics, prebiotics, Horse chestnut, flaxseed oil, colon cancer. PMID: 20568896 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695892 Fri, 25 Jun 2010 09:48:03 +0100 3695892 http://www.medworm.com/index.php?rid=3695891&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568897%26dopt%3DAbstract Authors: Klobusicka M, Kusenda J, Stevulova L, Kovarikova A, Babusikova O B-cell chronic lymphocytic leukemia (B-CLL) represents a heterogeneous disease with a very variable outcome. The reliable prognosis of this disease at the time of initial diagnosis is difficult to predict. The purpose of this preliminary study was to utilize the nucleolar morphology and to investigate the incidence of main nucleolar types in leukemic lymphocytes in B-CLL patients to assess their possible predictive value for the disease outcome, in correlation with immunophenotype parameters. The evaluation of nucleolar morphology of pathologic lymphocytes was performed at diagnosis and during the course of disease. Median follow up period of patients was 16.4 months (range from 2 to 32 months) from diagnosis. Th... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695891 Fri, 25 Jun 2010 09:48:03 +0100 3695891 http://www.medworm.com/index.php?rid=3695890&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568898%26dopt%3DAbstract We examined whether human leukemia HL-60 cells differentiating by all-trans retinoic acid (ATRA) acquired resistance to the apoptogenic activity of two histone deacetylase (HDAC) inhibitors, butyrate and valproate. In undifferentiated cells, the cytotoxicity of both butyrate and valproate was associated with activation of the intrinsic apoptotic pathway since we observed dissipation of mitochondrial membrane potential, induction of caspase-9 and caspase-3 activities, appearance of sub-G1 DNA and loss of plasma membrane asymmetry and/or integrity. Both HDAC inhibitors were also able to induce accumulation of undifferentiated cells in the G0/G1 phase of the cell cycle. ATRA was found to enhance the apoptotic effect of both butyrate and valproate in undifferentiated cells. This aside, ATRA ap... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695890 Fri, 25 Jun 2010 09:48:03 +0100 3695890 http://www.medworm.com/index.php?rid=3695889&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568899%26dopt%3DAbstract Authors: Votavova H, Forsterova K, Campr V, Sritesky J, Velenska Z, Pytlik R, Kubackova K, Prochazka B, Kodet R, Spicka I, Krejcova H, Trneny M, Klener P Primary mediastinal B-cell lymphoma (PMBL) seems to be reliably distinguished from diffuse large B-cell lymphoma (DLBCL) with microarray technology. We measured expression of Fcer2, Pdl2 and Blk genes using real-time quantitative polymerase chain reaction (RTqPCR) on formalin fixed, paraffin embedded material (FFPE) and suggested a formula to discriminate PMBL from DLBCL. For 39/82 included patients the diagnosis of PMBL was expected clinico-pathologically. Diagnosis of 10/39 and 2/43 of clinically considered PMBLs and DLBCLs, respectively, was not genetically confirmed. Compared to confirmed PMBLs, unconfirmed ones showed clinical fe... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695889 Fri, 25 Jun 2010 09:48:03 +0100 3695889 http://www.medworm.com/index.php?rid=3695888&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568900%26dopt%3DAbstract We present a method for the priming and expansion of myeloma-specific T lymphocytes using dendritic cells loaded with different types of tumor antigens. Cytotoxic T lymphocytes and/or activated dendritic cells generated by the described methods can be applied for cellular immunotherapy against multiple myeloma and other malignancies. Keywords: multiple myeloma, antitumor immunotherapy, dendritic cells, hTERT, MUC1, apoptotic bodies. PMID: 20568900 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695888 Fri, 25 Jun 2010 09:48:03 +0100 3695888 http://www.medworm.com/index.php?rid=3695887&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568901%26dopt%3DAbstract Authors: Celebiler Cavusoglu A, Sevinc AI, Saydam S, Canda T, Baskan Z, Kilic Y, Sakizli M We studied the promoter methylation status and expression levels of P16 and CDH1 genes in breast cancer and their adjacent normal tissues with normal control breast tissues, to correlate with their histopathological parameters. Hundred twenty four samples (tumor and adjacent nonmalignant tissues) from 62 breast cancer patients and 4 normal control breast tissues were included in the study. We used methylation specific PCR to evaluate methylation status and quantitative RT-PCR to measure the gene expression levels. Methylation incidence of P16 gene and CDH1 gene in tumor tissues were 24.2 % and 33.9 %, respectively. CDH1 and P16 gene were not methylated in normal control tissues. CDH1 underexpress... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695887 Fri, 25 Jun 2010 09:48:03 +0100 3695887 http://www.medworm.com/index.php?rid=3695886&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568902%26dopt%3DAbstract Authors: Stehlik P, Paulikova H, Hunakova L Isothiocyanates (ITCs), popular chemopreventive agents present in cruciferous vegetables, prove growth-inhibiting and apoptosis-inducing activities in cancer cell lines in vitro. Our study presents a new synthetic ITC derivate indol-3-ethyl isothiocyanate (homoITC) as an effective modulator of cellular proliferation and inducer of apoptosis with potential utility as an anticancer drug or a sensitizer to routinely used chemotherapeutic agent cisplatin (cis-Pt).<br />We analyzed the growth inhibitory effects of homoITC in the human ovarian carcinoma cell line A2780 and its cisplatin-resistant variant A2780/CP using MTT-test and its apoptosis-inducing properties by flow cytometry and caspase 3 activation. Combination index (CI) values from... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695886 Fri, 25 Jun 2010 09:48:03 +0100 3695886 http://www.medworm.com/index.php?rid=3695885&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568903%26dopt%3DAbstract Authors: Legartova S, Harnicarova-Horakova A, Bartova E, Hajek R, Pour L, Kozubek S Real time PCR is a powerful tool for studying the expression of genes involved in the pathophysiology of human diseases. Recent studies of the RAN (6p21), ZHX-2 (8q24.3), CHC1L (13q14.3) loci highlight the importance of these genes in multiple myeloma (MM) prognosis and therapeutic applications. Here, we described a detailed Real-Time PCR method for the detection of RAN, ZHX-2, and CHC1L expression, which could be applied in clinical situations. The expression profiles of these genes were studied in peripheral blood lymphocytes of healthy individuals, patients suffering from MM, and in the myeloma cell line, MOLP-8. Low expression levels of RAN, ZHX-2, and CHC1L were observed in myeloma patients, compar... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695885 Fri, 25 Jun 2010 09:48:03 +0100 3695885 http://www.medworm.com/index.php?rid=3695884&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568904%26dopt%3DAbstract Authors: Girard FP, Byrne J, Downes M, Fanning D, Desgrandchamps F, Fitzpatrick JM, Watson RW PSA, the only relevant marker for prostate cancer, has a low predictive value; moreover its low threshold leads to unnecessary biopsies with associated complications. Identification of prognostic factors is an important goal in prostate cancer. In the search for new markers, clusterin, has some potential as it is closely linked with cancer progression and resistance to apoptosis.<br />We looked at the expression of secreted clusterin (sCLU) in prostate cells to determine correlations with progression and drug resistance. The plasmatic expression of sCLU was also investigated in order to use it as a potential marker for prostate cancer. sCLU expression was studied using Western blotting o... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3695884 Fri, 25 Jun 2010 09:48:03 +0100 3695884 http://www.medworm.com/index.php?rid=3523057&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20429618%26dopt%3DAbstract Authors: Mydlikova Z, Gursky J, Pirsel M Transcription factor IIH (TFIIH) is eukaryotic multi protein complex identified in early 90's. Subsequent years have shown exceptional conservation of its structure from yeast to human. Although initially considered to be exclusively a basal transcription factor responsible for initiation of transcription and transition from initiation to elongation, TFIIH is also important in nucleotide excision repair for opening DNA at the site of a lesion and for recruitment of additional repair factors. Recently it was suggested that intact holocomplex of TFIIH is required for cell cycle regulation. Moreover, mutations in TFIIH subunits lead to three distinct genetic disorders: xeroderma pigmentosum (DNA repair disorder/cancer syndrome), Cockayne syndrome (... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3523057 Sun, 02 May 2010 00:16:05 +0100 3523057 http://www.medworm.com/index.php?rid=3523056&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20429619%26dopt%3DAbstract This article provides an up-to-date overview of tumors in which nestin has been found. Another aim of this review is to summarize recent findings on the intracellular localization of nestin in human tumor cells, especially with regard to the possible correlation between nestin expression and the malignant phenotype of transformed cells. Nestin expression in vascular endothelial cells during angiogenesis is also reviewed. Special attention is paid to the detection of nestin in cancer stem cells because this protein, together with the CD133 surface molecule, is considered to be a possible marker of cancer stem cells, especially in tumors of neuroectodermal origin. Keywords: nestin, intermediate filaments, cytoskeleton, human tumors, angiogenesis, cancer stem cells. PMID: 20429619 [PubMed... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3523056 Sun, 02 May 2010 00:16:05 +0100 3523056 http://www.medworm.com/index.php?rid=3523055&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20429620%26dopt%3DAbstract This study silenced HIF1-alpha, which is the regulatory subunit of HIF1, and established two dimensional gel electrophoresis profiles for human SW480siHIF1-alpha and SW480N cells to identified proteins with differential expressions. Ten down-regulated proteins and nine up-regulated proteins were identified by HPLC-Chip-MS/MS. Down-regulated proteins including PLD2, ANXA2. HIF1-alpha had a clear positive correlation with PLD2 and ANXA2 in colon adenocarcinoma samples. There proteins might have a direct or indirect contact HIF1-alpha, and play a critical role in apoptosis, signal transduction and hypoxia adaptation. Keywords: colorectal neoplasms; hypoxia inducible factor1-alpha; RNA interference; proteomics analysis; phospholipase D2; annexin A2. PMID: 20429620 [PubMed - in process] (So... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3523055 Sun, 02 May 2010 00:16:05 +0100 3523055 http://www.medworm.com/index.php?rid=3523054&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20429621%26dopt%3DAbstract In conclusions, we identified four genotypes significantly correlated with either the risk or some tumor characteristics, suggesting that the main selection criteria of the investigated SNPs (frequency and the position in modulating domains of the gene) are pertinent instruments for establish correlations between the gene structure and the tumor phenotype. Keywords: estrogen receptor alpha gene, single nucleotide polymorphisms, breast cancer. PMID: 20429621 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3523054 Sun, 02 May 2010 00:16:05 +0100 3523054 http://www.medworm.com/index.php?rid=3523053&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20429622%26dopt%3DAbstract Authors: Rudolf K, Cervinka M, Rudolf E The cytotoxicity of topoisomerase I inhibiting camptothecin, topoisomerase II inhibiting etoposide and their combination were investigated in wild type p53 Bowes and mutant p53 SK-MEL-28 melanoma cell lines during 24h. A combination of camptothecin and etoposide (1 mug/ml + 10 mug/ml) proved to be efficient in both types of cell lines, although mutant p53 cells exhibited a higher resistance. Further studies proved that in Bowes cells, a combination of drugs induced p53-dependent mitochondrial apoptosis characterized by activation of caspases-8 and -2, -9 and -3 with some concurrent involvement of oxidative stress. In SK-MEL-28 cells, apoptosis was found to be mediated via increased oxidative stress, activation of stress kinases such as p38 and SA... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3523053 Sun, 02 May 2010 00:16:05 +0100 3523053