MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Neoplasma' source. Sun, 21 Mar 2010 13:53:04 +0100 http://www.medworm.com/index.php?rid=3213336&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099971%26dopt%3DAbstract Authors: Pasz-Walczak G, Salagacka A, Potemski P, Balcerczak E, Kordek R, Mirowski M The aim of the study was to analyze the expression of Nm23-H1 and maspin proteins in a series of colorectal adenocarcinoma and to assess their applicability as prognostic factors in this type of cancer. 102 specimens of colorectal carcinoma were analyzed by immunohistochemistry with the use of anti-Nm23-H1 and anti-maspin monoclonal antibodies. Cytoplasmic expression of Nm23-H1 and maspin was found in 90 of all investigated cases. In 60 cases maspin protein was found also in nucleus. Medium/high Nm23-H1 cytoplasmic expression level was associated with tubular type of adenocarcinoma with deeper invasion of cancer into intestinal wall (T3, T4) and presence of vascular invasion. Medium/high expression lev... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213336 Thu, 28 Jan 2010 09:44:03 +0100 3213336 http://www.medworm.com/index.php?rid=3213335&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099972%26dopt%3DAbstract Authors: Scudla V, Pika T, Budikova M, Petrova J, Minarik J, Bacovsky J, Langova K, Zivna J The study aimed at evaluating the relation of 7 parameters associated with the internal biological properties of myeloma cells and the bone marrow microenvironment to multiple myeloma (MM) stages, distinguishing its initial/asymptomatic phase from monoclonal gammopathy of undetermined significance (MGUS) and assessing their relation to myeloma prognosis. In the studied group comprising 286 individuals (89 MGUS and 179 MM patients), statistically significant differences (Mann-Whitney test) between MGUS and MM at the time of diagnosis were found in the serum levels of HGF (hepatocyte growth factor), VEGF (vascular endothelial growth factor), ICTP (intercellular - carboxy-terminal telopeptide of ty... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213335 Thu, 28 Jan 2010 09:44:03 +0100 3213335 http://www.medworm.com/index.php?rid=3213334&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099973%26dopt%3DAbstract Authors: Greslikova H, Zaoralova R, Filkova H, Nemec P, Oltova A, Kupska R, Rudolecka P, Smetana J, Pour L, Zahradova L, Krejci M, Buchler T, Adam Z, Hajek R, Kuglik P Malignant plasma cells in multiple myeloma (MM) are frequently characterized by complex karyotypes and chromosome instability. These cytogenetic changes are considered important prognostic indicators in MM patients. We have studied samples from 68 patients with newly diagnosed MM who were treated with high-dose chemotherapy and autologous stem cell transplantation. G-banding revealed abnormal karyotypes in 14 of 55 patients (25%) who had informative conventional cytogenetics. The combination of cytoplasmic immunoglobulin light chain labeling and interphase fluorescent in situ hybridization (cIg-FISH) revealed the presenc... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213334 Thu, 28 Jan 2010 09:44:03 +0100 3213334 http://www.medworm.com/index.php?rid=3213333&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099974%26dopt%3DAbstract Authors: Tajtakova M, Pidanicova A, Valansky L, Lachvac L, Nagy V, Sivonova M, Dobrota D, Kliment J, Petrovicova J The aim of the paper is to determine whether IGF1, IGFBP3 and IGF1/IGFBP3 molar ratio in addition to PSA and one-nucleotide polymorphism in PSA and CYP17 gene might contribute to early diagnostics of prostate cancer (PCa). Serum level of PSA, IGF1 and IGFBP3 in the group of 158 individuals (92 PCa and 66 controls) was examined by RIA method and IGF1/IGFBP3 was calculated. PCR RLFP method was used to examine one- nucleotide polymorphism in PSA and CYP 17 gene. The results suggest that serum level of IGF1 over 95% CI did not increase relative risk of PCa development in overall group, not even regarding to particular investigated genotypes, not even if individuals with genoty... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213333 Thu, 28 Jan 2010 09:44:03 +0100 3213333 http://www.medworm.com/index.php?rid=3213332&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099975%26dopt%3DAbstract Authors: Pan Z, Chen S, Pan X, Wang Z, Han H, Zheng W, Wang X, Li F, Qu S, Shao R Cervical cancer is one of the most common gynecological cancers worldwide. Over the past decade, much progress has been made in understanding the genetic changes associated with the development and progression of cervical cancer. However, the precise mechanisms of cervical carcinogenesis in Uigur women remain unclear. To screen differential gene expression in squamous cell carcinoma (SCC) of the cervix in Uigur women, suppressive subtractive hybridization (SSH) was performed on the cervical squamous cell carcinoma and corresponding normal cervical tissues of a Uigur patient. Thus we were be able to find the genes that are related with cervical tumors of Uigur women. A total of 300 samples were subject to ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213332 Thu, 28 Jan 2010 09:44:03 +0100 3213332 http://www.medworm.com/index.php?rid=3213331&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099976%26dopt%3DAbstract Authors: Lu Y, Xie D, Huang W, Gong H, Yu J The aim of this study was to assess the usefulness of integrated 18F-FDG PET/CT in differentiating benign from metastatic malignant adrenal masses in patients with lung cancer. <br />One hundred and ten adrenal masses (size range, 0.5 - 6.3 cm, mean size, 1.9 cm) were evaluated retrospectively in 87 lung cancer patients. Integrated PET/CT images were assessed. PET findings were interpreted as positive if the 18F-FDG uptake of the adrenal mass was greater than or equal to that of the liver. PET findings were interpreted as negative if the 18F-FDG uptake of the adrenal mass was less than that of the liver. All studies were reviewed independently by 3 nuclear medicine physicians, and the results were then correlated with clinical follow-up... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213331 Thu, 28 Jan 2010 09:44:03 +0100 3213331 http://www.medworm.com/index.php?rid=3213330&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099977%26dopt%3DAbstract Authors: Radosavljevic G, Ljujic B, Jovanovic I, Srzentic Z, Pavlovic S, Zdravkovic N, Milovanovic M, Bankovic D, Knezevic M, Acimovic L, Arsenijevic N The promotion of tumor growth is due to a combination of several mechanisms, including angiogenesis and the abundance of cell-derived inflammatory cytokines. The aim of this study was to investigate the serum levels of interleukin 17 (IL-17) and the expression of p53 and Vascular Endothelial Growth Factor (VEGF), in order to determine the relationship between these markers and serum IL-17 levels in patients with colorectal carcinoma. <br />Serum levels of the proinflammatory cytokine IL-17 in patients with colorectal carcinoma (CRC) (n=40) and in a healthy group (n=37) were analysed by ELISA. Surgically resected specimens of 59 co... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213330 Thu, 28 Jan 2010 09:44:03 +0100 3213330 http://www.medworm.com/index.php?rid=3213329&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099978%26dopt%3DAbstract Authors: Osada J, Rusak M, Kamocki Z, Dabrowska MI, Kedra B The aim of this study was to evaluate platelet activation in gastric cancer patients with regard to histopathological classification and the presence of distant metastases, by using platelet morphological parameters: MPV, L-PLT, MPC, as well as quantitative evaluation of surface receptor expression: CD41a, CD61, CD42b, CD62P, by flow cytometry at the resting state and after TRAP activation.<br />In gastric cancer patients higher values of MPV and LP, as well as decreased MPC values were determined. Quantitative evaluation of surface antigen expression also revealed higher number of CD41a, CD61 and CD62P molecules, as compared with the platelets in the control group.<br />Significant decrease of CD42b molecules' num... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213329 Thu, 28 Jan 2010 09:44:03 +0100 3213329 http://www.medworm.com/index.php?rid=3213328&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099979%26dopt%3DAbstract Authors: Konopacka M, Rogoliński J Using X radiation commonly used in radiotherapy of cancers we investigated bystander interactions between human cells: irradiated A549 bronchial carcinoma human cells and non irradiated BEAS-2B normal bronchial epithelial cells. Non irradiated cells were incubated in medium transferred from irradiated A549 cells (ICM-irradiation conditioned medium) for 48h and next the chromosomal damage and apoptosis were estimated. Conditioned medium collected from irradiated cancer cells induced in non irradiated cells of the same line as well as in BEAS-2B normal cells genetic changes such as micronuclei, chromatid and chromosomal breaks and condensation of chromatin characteristic for processes of apoptosis. Addition of only 1% of conditioned medium to fres... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213328 Thu, 28 Jan 2010 09:44:03 +0100 3213328 http://www.medworm.com/index.php?rid=3213327&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099980%26dopt%3DAbstract In conclusion we demonstrated the feasibility and safety of anaesthesia applied in our radiotherapy patients. The custom designed mobile anaesthesia workstation allowed us to provide safe and efficient anaesthesia in any place outside the Department of Anaesthesiology. Keywords: anaesthesia for radiotherapy, paediatric radiotherapy, brachytherapy, intraoperative radiotherapy, childhood cancers. PMID: 20099980 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213327 Thu, 28 Jan 2010 09:44:03 +0100 3213327 http://www.medworm.com/index.php?rid=3213326&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099981%26dopt%3DAbstract Authors: Korinkova G, Cwiertka K, Paprskarova M, Dzubak P, Hajduch M Bohemine and roscovitine are the most important representatives of the group of compounds structurally derived from olomoucine. Biologically they function as inhibitors of cyclin-dependent kinases (CDKs), the key regulators of cell cycle, which is often disrupted in cancer cells resulting in uncontrollable proliferation. Bohemine and roscovitine have demonstrated their cytostatic and cytotoxic in vitro and also in vivo effects. Currently the phase II clinical trials for roscovitine are underway. The aim of the study was to evaluate the potential in vitro radiosensitising effect of bohemine (BOH) and roscovitine (ROS).<br />Clonogenic survival assay and human lung adenocarcinoma cell line A549 were used. Tested s... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213326 Thu, 28 Jan 2010 09:44:03 +0100 3213326 http://www.medworm.com/index.php?rid=3213325&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099982%26dopt%3DAbstract Authors: Szotkowski T, Rohon P, Zapletalova L, Sicova K, Hubacek J, Indrak K Secondary acute myeloid leukemia (sAML) may arise from the previous clonal disorder of hematopoiesis, usually from myelodysplastic syndrome (MDS) or from chronic myeloproliferative neoplasia (cMPN) or after exposure to a leukemogenic agent (previous chemotherapy or radiotherapy, some immunosuppressive drugs or environmental leukemogenic agents). Secondary origin of AML is associated with unfavorable prognosis and it is not considered to be conventionally curable (with the exception of secondary acute promyelocytic leukemia). <br />The presented study is a retrospective analysis of patients diagnosed and treated at the Department of Hemato-Oncology, University Hospital Olomouc in 1996-2008.<br />Ove... Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213325 Thu, 28 Jan 2010 09:44:03 +0100 3213325 http://www.medworm.com/index.php?rid=3213324&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099983%26dopt%3DAbstract In conclusion, differences in NT-proBNP values between patients treated with anthracyclines and healthy volunteers might signal an initial stage of anthracycline-induced myocardial damage. The potential of this biomarker to detect subclinical anthracycline-induced myocardial alterations before development of echocardiographic and clinical changes is promising. Keywords: PMID: 20099983 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213324 Thu, 28 Jan 2010 09:44:03 +0100 3213324 http://www.medworm.com/index.php?rid=3213323&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099984%26dopt%3DAbstract In conclusion, these results suggest that it is possible to completely cure animals bearing advanced tumors, with a combined therapy, PDT + adoptive immunotherapy + low dose chemotherapy. Keywords: photodynamic therapy, combined therapy, immunotherapy, chemotherapy, L1210 murine leukemia. PMID: 20099984 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=3213323 Thu, 28 Jan 2010 09:44:03 +0100 3213323 http://www.medworm.com/index.php?rid=2978351&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895165%26dopt%3DAbstract Authors: Maric DM, Jovanovic DM, Golubicic IV, Nagorni-Obradovic LJ, Stojsic JM, Pekmezovic TD Patients with advanced non-small cell lung cancer (NSCLC) usually undergo toxic treatment (chemotherapy and/or radiotherapy). They can experience devastating effects of illness and therapies on their psychological and emotional well-being. On the other hand, untreated psychological distress is associated with reduced quality of life and inadequate palliation of physical symptoms.<br />In order to estimate frequency of anxiety and depressive symptoms and influence of demographic, socioeconomic and clinical factors on psychological well-being, we performed this cross-sectional study in group of 100 patients with advanced stage of disease. Symptoms of anxiety and depression were assessed u... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978351 Wed, 11 Nov 2009 11:46:39 +0100 2978351 http://www.medworm.com/index.php?rid=2978350&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895166%26dopt%3DAbstract Authors: Minarik J, Scudla V, Bacovsky J, Zemanova M, Pika T, Ordeltova M, Langova K We analyzed proliferative index of myeloma plasmocytes (PC-PI) in acohort of 217 patients with multiple myeloma (MM) treated with conventional chemotherapy and biological agents, thalidomide and bortezomib. In the whole group was adifference between overall survival (OS) favoring patients with PC-PI ven after 40 months (median overall survival 25 vs 10months, p= 0.015), whereas in the group treated with thalidomide and bortezomib was no difference, with medians over 39 months. Even patients with low PC-PI profited from the treatment with novel drugs. Presented results suggest that the treatment of MM with novel agents overcomes the prognostic significance of PC-PI and should be used in all MM patients.... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978350 Wed, 11 Nov 2009 11:46:36 +0100 2978350 http://www.medworm.com/index.php?rid=2978349&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895167%26dopt%3DAbstract Authors: Vrana D, Novotny J, Holcatova I, Hlavata I, Soucek P Pancreatic cancer represents one of the biggest problems of current oncology. The risk factors of pancreatic cancer development, as well as factors affecting survival are poorly understood. Since biotransformation enzymes modify detoxification of carcinogens, we supposed, that arelationship between their polymorphism and the risk of pancreatic cancer development and eventually its clinical outcome may exist.<br />Associations of so far not studied cytochrome P450 1B1 (CYP1B1) polymorphisms with pancreatic cancer risk were investigated by case-control study. Atotal of 754 participants were recruited during study period. All patients were followed to determine their treatment and overall survival.<br />Carriers of ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978349 Wed, 11 Nov 2009 11:46:33 +0100 2978349 http://www.medworm.com/index.php?rid=2978348&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895168%26dopt%3DAbstract In conclusion, although there seems to be adirect link between cancer invasion and MMP9 immunohistochemical expression, the role of MMP2 and MMP7 appears to be more complicated underlining the complexity of the mechanisms involved in cancer spreading. Keywords: PMID: 19895168 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978348 Wed, 11 Nov 2009 11:46:31 +0100 2978348 http://www.medworm.com/index.php?rid=2978347&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895169%26dopt%3DAbstract Authors: Pour L, Svachova H, Adam Z, Mikulkova Z, Buresova L, Kovarova L, Buchler T, Penka M, Vorlicek J, Hajek R Our aim was to establish whether the pretreatment levels of angiogenesis activators and inhibitors can be used to predict clinical responses to treatment that included high-dose chemotherapy with peripheral stem cell support.<br />We analyzed samples and treatment outcomes of 96 patients with MM enrolled in the CMG 2002 randomized clinical trial and treated with induction chemotherapy and high-dose chemotherapy with stem cell support. Concentrations of vascular endothelial growth factor (VEGF), hepatocytar growth factor (HGF), basic fibroblastic growth factor (bFGF), thrombospondin-1 (TSP-1), endostatin, and angiostatin were measured in the peripheral blood plasma and... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978347 Wed, 11 Nov 2009 11:46:28 +0100 2978347 http://www.medworm.com/index.php?rid=2978346&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895170%26dopt%3DAbstract Authors: Lasabova Z, Tilandyova P, Kajo K, Zubor P, Burjanivova T, Danko J, Plank L Breast cancer is one of the most common cancer affecting women and the recent research is focused on identifying new genetic and epigenetic prognostic and predictive factors. Glutathione S-transferase P1 (GSTP1) is abiotransformation enzyme expressed in normal breast epithelial cells which can be epigenetically inactivated in breast cancer. We have shown, that application of nested two-stage methylation-specific PCR (MSP) is asuitable method for analysis of epigenetically silenced GSTP1 in formalin-fixed paraffin-embedded (FFPE) tissues from breast cancer patients. Of 45 breast tumors, 11 (24, 4%) were found to have methylated GSTP 1promoter region. We were able to demonstrate the correlation between th... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978346 Wed, 11 Nov 2009 11:46:26 +0100 2978346 http://www.medworm.com/index.php?rid=2978345&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895171%26dopt%3DAbstract Authors: Grzanka AA, Placek W, Grzanka A, Sokolowska-Wojdylo M, Zuryn A Mycosis fungoides is an epidermotropic cutaneous T-cell lymphoma (CTCL).Specimens for presented study were taken from sixteen patients with MF confirmed by immunohistochemical methods and PCR and from nine patients with benign dermatoses. To demonstrate CD3, CD4 and CD7 antigens immunogold method was used. We saw morphological differences between lymphocytes from MF and benign dermatoses. In MF, CD3 and CD4 were present rather in form of clusters placed on the surface of cell. On the contrary -CD3 to CD7 distribution analysis showed that these antigens were present rather individually, however there were seen clusters as well. In MF tumor stage labelling decreased in following order: CD7, CD3 and CD4. We also found... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978345 Wed, 11 Nov 2009 11:46:23 +0100 2978345 http://www.medworm.com/index.php?rid=2978344&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895172%26dopt%3DAbstract In conclusion, altered nucleotide sequence and repression of transcription are two potential mechanisms of suppression of the anti-metastatic effects of KiSS1 in early breast cancer: Confirmation in larger cohorts and study of functional effects of the 242C>G exon IVa mutation are warranted. Keywords: KiSS1, metastasis-suppressor gene, breast cancer, mutation, transcription. PMID: 19895172 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978344 Wed, 11 Nov 2009 11:46:20 +0100 2978344 http://www.medworm.com/index.php?rid=2978343&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895173%26dopt%3DAbstract Authors: Jing R, Cui M, Wang J, Wang H Receptor for advanced glycation end products (RAGE) may be involved in the pathogenesis of cancer progression. Pathological effects mediated via RAGE are physiologically inhibited by soluble RAGE (sRAGE). The aim of this study was to identify sRAGE and RAGE expression profile in lung cancer patients. An ELISA method was used to quantify serum sRAGE in 45 individuals. Additionally, surgical specimens of 28 lung cancer patients were also included for RAGE expression by immunohistochemistry. Serum sRAGE was significantly decreased in lung cancer patients compared with controls (vs. healthy donors, P=0.034; vs. pulmonary tuberculosis patients, P=0.010). Lower sRAGE concentration was negative correlated with lymph node involvement (N0 vs. N1-2, P=0.028... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978343 Wed, 11 Nov 2009 11:46:18 +0100 2978343 http://www.medworm.com/index.php?rid=2978342&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895174%26dopt%3DAbstract Authors: Guo WC, Zhao SH, Yu L, Tang J, Wu GX, Chen JL The aim of the study was to observe the expression of heat shock protein gp96 (HSPgp96) and explore its clinical significance in human osteosarcoma. <br />The expression of HSPgp96 was studied in 44 osteosarcoma tissues including 24 osteoblastic sarcoma and 20 chondroblastic sarcoma, normal tissues adjacent to the sarcomas were evaluated simultaneously. <br />The immunoreactivity was found positive in all osteosarcoma tissues (44/44), but 21.5% (9/44) in normal tissues. HSPgp96 was mainly expressed in cytoplasm of osteoblastic sarcoma, while in nucleus of chondroblastic sarcoma. HSPgp96 immunolabelling had significantly correlation with the Price degree (P 0.05), and histological subtypes (P >0.05). <br />The H... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978342 Wed, 11 Nov 2009 11:46:15 +0100 2978342 http://www.medworm.com/index.php?rid=2978341&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895175%26dopt%3DAbstract Authors: Pavlovic S, Artiko V, Sobic-Saranovic D, Damjanovic S, Popovic B, Jakovic R, Petrasinovic Z, Jaksic E, Todorovic-Tirnanic M, Saranovic D, Micev M, Novosel S, Nikolic N, Obradovic V Our aim was to assess clinical utility of 99mTc-EDDA/HYNIC-TOC scintigraphy for evaluation of lung lesions in patients with neuroendocrine tumors (NETs). Single photon emission computed tomography (SPECT) of the thorax and whole body scintigraphy were performed in 34 patients using 99mTc-EDDA/HYNIC-TOC. Visual assessment was complemented by semiquantitative evaluation based on tumor to non-tumor (T/NT) ratio. Clinical, laboratory, and histological findings served as the standard for comparison. Enhanced tracer uptake was observed on both SPECT and whole body scintigraphy in 29 of 34 patients (88% se... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978341 Wed, 11 Nov 2009 11:46:13 +0100 2978341 http://www.medworm.com/index.php?rid=2978340&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895176%26dopt%3DAbstract Authors: Raheem SN, Atoum M, Al-Hourani H, Rasheed M, Nimer N, Almuhrib T Hormones such as estrogen, progesterone and prolactin are implicated in anumber of ways as possible causes of breast cancer. Throughout women life cycle, breast development and function depend on complex critical interplay of these hormones. The acknowledged gaps in our understanding concerning progesterone, estrogen and prolactin hormones involvement in human breast cancer has exposed the need to conduct this study for better understanding of the role played by these hormones in breast cancer during pre and post menopause status in order to influence prevention and treatment of breast cancer. Ninety women were enrolled, (80%) of them were breast cancer patients and the other (20%) were breast benign lesion patie... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978340 Wed, 11 Nov 2009 11:46:10 +0100 2978340 http://www.medworm.com/index.php?rid=2978339&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895177%26dopt%3DAbstract We examined the effect of PDT using HMME as the photosensitizer, and the 630nm diode laser on human cholangiocarcinoma cell line QBC939. Cell viability was determined by MTT assay. The percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Two methods were used for the determination of apoptosis: terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling assay and laser scanning confocal microscope detection. The procaspase-3 and cytochrome cwere measured by western blot. In vitro PDT showed excellent cytotoxicity that was afunction of laser energy and drug concentration to the QBC939 cell lines. PDT-mediated cell death occurred predominantly by apoptosis in vitro. Furthermore, this treatment initiates early cytochrome crelease, foll... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978339 Wed, 11 Nov 2009 11:46:07 +0100 2978339 http://www.medworm.com/index.php?rid=2978338&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895178%26dopt%3DAbstract In this study, the correlation between the HO-1 gene promoter polymorphism and alcohol, along with the risk of ESCC on Chinese males, was analyzed.The case-control study was performed in 143 ESCC patients and 264 cancer-free controls. All subjects were males. Allelotypic frequencies of (GT)n repeat were examed by PCR-based genotyping and DNA sequencing. The frequencies of L-allele and L-allele carriers (S/L and L/L genotypes) was significantly higher in ESCC patients than in controls (p =0.001 and 0.004), The adjusted ORs for ESCC with S/L and L/L genotypes vs S/S genotype was 2.212 (95% CI 1.297-3.775, p= 0.004). The adjusted ORs for light, moderate and heavy drinking was 1.467, 5.215 and 9.525 respectively among L-allele carriers (S/L and L/L genotypes )and 1.389, 2.096 and 3.039 respect... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2978338 Wed, 11 Nov 2009 11:46:05 +0100 2978338 http://www.medworm.com/index.php?rid=2948888&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19873686%26dopt%3DAbstract This study was local prospective and cross-sectional. It was carried at Department of Clinical Oncology and Radiation Therapy of Charles University Hospital in Hrader Králové, Czech Republic. Dates were obtained during year 2008 among 41 patients with metastatic breast cancer in programme of palliative cancer care. The mean age for all 41 subjects was 58 years old (aged 41 - 80 years old). The Czech version of Zung self-rating depression scale was performed for evaluation of depression symptoms. The Czech version of genetic EuroQol questionnaire EQ-5D was performed for evaluation of level of HRQoL. The statistical evaluation presents that mean ZSDS (Zung self-rating depression score) certifies the presence of signs of moderately depression symptoms among patients with metastatic ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2948888 Mon, 02 Nov 2009 05:28:06 +0100 2948888 http://www.medworm.com/index.php?rid=2768990&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728753%26dopt%3DAbstract Authors: Ondrusova M, Ondrus D, Dusek L, Spanikova B Improved survival of testicular cancer (TC) patients leads to rising of interest on the disease consequences for the whole organism (impact on hormonal status, bone metabolism). The aim of the study was to present three years experience with hormonal and osteologic examination in long-term survivors of TC. During the period of 11/2005-1/2009 879 patients who were previously treated for TC (823 with unilateral, 56 with bilateral disease) were examined. Each patient underwent hormonal and osteologic examination, results of which were associated with therapy following orchiectomy and with the time interval since the primary therapy. The median follow-up time in patients with unilateral TC was 96 months since the therapy. Mean age at the... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768990 Sat, 05 Sep 2009 22:16:02 +0100 2768990 http://www.medworm.com/index.php?rid=2768989&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728754%26dopt%3DAbstract Authors: Fridrichova M, Dienstbier Z, Loucka M, Skala E, Blomannova E The long-term survival in a group of 370 patients treated for Hodgkin's disease in the years 1971-1996 was retrospectively analyzed. Up to now 191 patients live. 179 patients have died.<br />Since the year 1978 the uniform diagnostic and therapy protocol has been used. The therapy consisted in the combination of a radiation therapy (usual dose 40 Gy) with the COPP chemotherapy (6 cycles). Since the year 1988 the alternation of the ABVD and COPP chemotherapy has been used. According to the stage of the Hodgkin's disease and the patient's age the modification of the therapy was introduced. One chemotherapy cycle was removed for each 10 years above the 50 year age of the patient. The radiation therapy was not appl... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768989 Sat, 05 Sep 2009 22:16:02 +0100 2768989 http://www.medworm.com/index.php?rid=2768988&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728755%26dopt%3DAbstract Authors: Wachsmannova-Matelova L, Stevurkova V, Adamcikova Z, Holec V, Zajac V Germline mutation in APC gene induced development of familial adenomatous polyposis (FAP). The risk of developing specific manifestation of FAP is often correlated with the position of the inherited APC mutation. Patients with mutations localized in the largest exon 15 between codons 1286 and 1513 (mutation cluster region, MCR) have generally a worse prognosis with early onset of the disease. We found 6 FAP families with mutation at codon 1309 (3927_3931delAAAGA) in the cohort of 39 FAP Slovak families with rapid cancer progress. In addition, mutation in codon 1309 was detected in three family members, one of them with a very different phenotype. This oldest family member, aged 81, has persisted asymptomatic... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768988 Sat, 05 Sep 2009 22:16:02 +0100 2768988 http://www.medworm.com/index.php?rid=2768987&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728756%26dopt%3DAbstract Authors: Varamini P, Soltani M, Ghaderi A There are reports on the presence of various compounds exerting different biological activities in Ruta graveolens, a plant of Rutaceae family. The aim of the present study was to evaluate in vitro cytotoxicity of the total extract of R. graveolens against tumor cell lines of different origin. Aerial parts of the plant was extracted with 70% ethanol by sonication method and cytotoxic activity was examined on RAJI, RAMOS, RPMI8866, U937, Jurkat, MDA-MB-453, MCF-7, LNCap-FGC-10, 5637, HeLa, SK-OV-3, A549, Mehr-80 and also peripheral blood mononuclear cells (PBMC) by the use of WST-1 assay. Results were expressed as IC50 values. R. graveolens extract showed high cytotoxic activity against RAJI and RAMOS, two Burkitt's lymphoma cell lines, with an ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768987 Sat, 05 Sep 2009 22:16:02 +0100 2768987 http://www.medworm.com/index.php?rid=2768986&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728757%26dopt%3DAbstract Authors: Jantova S, Repicky A, Cipak L Quinazoline derivatives are multitarget agents with a broad spectrum of biological activity. 3-(5-nitro-2-thienyl)-9-chloro-5-morpholin-4-yl[1,2,4]triazolo[4,3-c]quinazoline (NTCHMTQ) is a new synthetically prepared derivative, which in our previous studies showed antiproliferative and apoptosis inducing activities towards murine L1210 leukemia cells. The aim of this study was to provide the insight into the molecular mechanism regulating NTCHMTQ-induced apoptosis in L1210 cells. The activity of caspases 3, 8 and 9, generation of reactive oxygen species (ROS), mitochondrial membrane potential changes, release of cytochrome c, degradation of PARP and activation of c-Jun N-terminal kinase 1/2 (JNK1/2), p38 MAPK and extracellular-regulated kinase 1/2... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768986 Sat, 05 Sep 2009 22:16:02 +0100 2768986 http://www.medworm.com/index.php?rid=2768985&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728758%26dopt%3DAbstract In conclusion, germline disruption of the PML gene is probably not associated with a highly penetrant susceptibility to adult-onset breast and colon cancer. Pathogenicity of c.83C>T and c.1558C>T variants in the PML gene is uncertain. Carriers of the c.2260 G>C variant in PMLIV isoform may be at an increased risk of colon polyposis and cancer. Keywords: breast cancer; cancer risk; colon cancer; colon polyposis; germline mutation; PML gene. PMID: 19728758 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768985 Sat, 05 Sep 2009 22:16:02 +0100 2768985 http://www.medworm.com/index.php?rid=2768984&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728759%26dopt%3DAbstract Authors: Babusikova O, Stevulova L, Fajtova M The main aim of this study represents the extension of our studies using multiparametric flow cytometry analysis for exact definition of membrane and intracellular (cytoplasmic and nuclear) markers of acute leukemia cells of T-phenotype. The study of blasts of each patient with all available monoclonal antibodies targeted to T-cell differential antigens and against possible marker coexistence from different lineages has been performed. The main aim was concerned to more proper T-ALL diagnosis and stage definition and identification of the prognostic factors and the useful markers for the follow-up of T-ALL in remission. New knowledge of the T-cell maturation stages of hematopoietic cells in bone marrow and thymus has been applied, as each T... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768984 Sat, 05 Sep 2009 22:16:02 +0100 2768984 http://www.medworm.com/index.php?rid=2768983&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728760%26dopt%3DAbstract Authors: Polakova K, Bandzuchova E, Sabty FA, Mistrik M, Demitrovicova L, Russ G Human leukocyte antigen - G (HLA-G) is a non-classical HLA class I antigen with restricted distribution in normal tissues. Ectopic HLA-G expression observed at some pathological circumstances as malignant transformation might be triggered by epigenetic modifications such as DNA demethylation. Recently it was demonstrated that DNA methyltransferase inhibitor 5-aza-2 - deoxycytidine (AdC) induces/enhances HLA-G transcription in many leukemia cell lines of different origin. Here we investigated the effect of AdC on HLA-G expression in malignant hematopoetic cells isolated from patients with acute myeloid leukemia (AML) and chronic lymphocytic leukemia (B-CLL). We detected HLA-G expression in untreated cells f... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768983 Sat, 05 Sep 2009 22:16:02 +0100 2768983 http://www.medworm.com/index.php?rid=2768982&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728761%26dopt%3DAbstract In this study we confirmed a higher prevalence of symptoms characteristic for different metabolic syndromes in these patients with adrenal incidentalomas, which indicate systematic screening for the metabolic syndrome including evaluation of the insuline resistance in this patients. Keywords: adrenal incidentaloma, cholesterol, triacyglyceroles, obesity, hypertension, steroid enzymopathy. PMID: 19728761 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768982 Sat, 05 Sep 2009 22:16:02 +0100 2768982 http://www.medworm.com/index.php?rid=2768981&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728762%26dopt%3DAbstract Authors: Kovarova L, Buresova I, Buchler T, Suska R, Pour L, Zahradova L, Penka M, Hajek R Flow cytometry is a useful tool for the analysis of plasma cells in monoclonal gammopathies. The aim of this study was to find possibilities and limits of multicolour flow cytometry in diagnostics of monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) and to identify parameters that could be used to differentiate between these two disorders. Surface markers CD38 and CD138 were used for identification of plasma cells, CD19 and CD56 further distinguished normal and abnormal plasma cells, respectively. The percentage of circulating plasma cells in peripheral blood was lower in MGUS patients then in MM (p Keywords: plasma cells, multiple myeloma, monoclonal gammopathy,... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768981 Sat, 05 Sep 2009 22:16:02 +0100 2768981 http://www.medworm.com/index.php?rid=2768980&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728763%26dopt%3DAbstract In conclusion, Notch1/Jagged1 were frequently low expressed in hepatocellular carcinoma and correlated with the high expression of beta-catenin suggesting that downregulation of Notch1/Jagged1 signaling may sustain tumor progression. Keywords: Notch1, Jagged, beta-catenin; hepatocellular carcinoma, tissue microarrays, immunohistochemistry. PMID: 19728763 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768980 Sat, 05 Sep 2009 22:16:02 +0100 2768980 http://www.medworm.com/index.php?rid=2768979&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728764%26dopt%3DAbstract Authors: Altanerova V, Horvathova E, Matuskova M, Kucerova L, Altaner C Human adipose tissue-derived mesenchymal (stromal) stem cells (AT-MSCs) and genetically modified to express cytosine deaminase:uracil phosphoribosyltransferase (CDy-AT-MSCs) were treated with hydrogen peroxide in order to induce DNA damage and subsequently evaluate their genetic stability by single cell gel electrophoresis. Both cells types (parental and transgene modified) did not differ in the sensitivity to DNA breaks induction. Potential tumorigenicity of AT-MSCs and CDy-AT-MSCs was tested by subcutaneous inoculation of cell suspension into flank of immunocompromised mice. Dose of 15x106 cells was not found to be tumorigenic in given experimental setup. AT-MSCs, CDy-AT-MSCs and MSCs isolated from human lipoma w... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768979 Sat, 05 Sep 2009 22:16:02 +0100 2768979 http://www.medworm.com/index.php?rid=2768978&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728765%26dopt%3DAbstract In this study, we describe effect of SFN on various aspects determining invasive behavior of MDA-MB-231 human breast carcinoma cells. <br />We studied modulation of molecules associated with epithelial to mesenchymal transition (EMT), hypoxic marker CA IX and mitochondrially located peripheral benzodiazepine receptor (PBR) using flow cytometry, gene expression of matrix metalloproteinases MMP1, 3, 7, 9, 14, transcription factors POU5F1 and Twist1 mRNA by RT PCR, and cytokine production by multiplex bead assay.<br />SFN downregulated PBR and vimentin expression in a dose dependent manner, but significantly affected neither HIF-1alpha, nor CA IX protein expression, nor VEGF and GLUT1 mRNA levels. Among studied MMPs, MMP7 and MMP14 mRNA were downregulated while no apparent effect ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2768978 Sat, 05 Sep 2009 22:16:02 +0100 2768978 http://www.medworm.com/index.php?rid=2581210&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580337%26dopt%3DAbstract Authors: Wang YH, Li F, Luo B, Wang XH, Sun HC, Liu S, Cui YQ, Xu XX We used flow cytometry and a DNA-binding dye efflux assay to isolate a side population (SP) of cells with stem cell characteristics from the human pancreatic carcinoma cell line, PANC-1. Non-obese diabetic/severe combined immunodeficiency mouse xenograft experiments showed that SP cells were enriched in tumor initiating capability compared with non-SP cells. Cultured SP cells were able to differentiate into daughter cells and non-SP cells, through asymmetric division. Our study demonstrated that SP cells had high drug-resistance, both in vivo and in vitro. SP cells also showed significantly higher levels of mRNA expression for CD133, ABCG2 and Notch1, when compared to non-SP cells. Furthermore, xenografted tumors deri... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581210 Wed, 08 Jul 2009 23:12:03 +0100 2581210 http://www.medworm.com/index.php?rid=2581209&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580338%26dopt%3DAbstract Authors: Souckova K, Kovarik A, Dusek L, Humpolikova-Adamkova L, Lauerova L, Krejci E, Matouskova E, Bursikova E, Fojtova M, Kovarik J The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation to SOCS3 expression in a panel of breast cancer cell lines and normal mammary epithelial cells. Compared to normal cells most breast cancer lines (7/8) were highly resistant to IFN-gamma. Using Northern blot and real time RT-PCR we investigated transcription of SOCS3 genes. All normal epithelial cells (4/4) showed SOCS3 induction (2-14 fold) while most breast cancer lines did not or weakly activated SOCS3 after the interferon gamma treatment. Among the cancer lines, the MDA-MB-468 cells showed in... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581209 Wed, 08 Jul 2009 23:12:03 +0100 2581209 http://www.medworm.com/index.php?rid=2581208&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580339%26dopt%3DAbstract Authors: Zhong X, Wu B, Pan YJ, Zheng S Hepatocellular carcinomas represent the third leading cause of cancer-related deaths worldwide. Survivin, a structurally unique member of the inhibitor of apoptosis protein (IAP) family, is overexpressed in a wide range of malignancies, including hepatocellular carcinoma. Due to its involvement in cancer progression and treatment resistance, survivin is currently undergoing extensive investigation as a novel intervention target to induce apoptosis in cancer cells by phytochemicals or synthetic agents. Brazilein, a compound obtained in a large amount from the dried heartwood of Caesalpinia sappan Linn., which has long been used in traditional medicine in China, has some pharmacological activities. Human hepatocellular carcinoma HepG2 cells were tr... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581208 Wed, 08 Jul 2009 23:12:03 +0100 2581208 http://www.medworm.com/index.php?rid=2581207&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580340%26dopt%3DAbstract Authors: Otahalova E, Ullmannova-Benson V, Klamova H, Haskovec C The determination of patient's resistance to a particular drug contributes to more efficient therapeutical approach. The aim of this study was to evaluate if the responsiveness of Chronic Myeloid Leukemia (CML) patients to Imatinib therapy was predictable from WT1 gene expression in peripheral blood leukocytes. To examine the resistance we implemented an in vitro cultivation of the primary cells of 48 CML patients with Imatinib. The effect of Imatinib was characterized not only by the expression of WT1 but also by BCR-ABL, and proliferative factor Ki-67. <br />Our results showed that leukocytes of CML patients, clinically responsive to Imatinib treatment, significantly decreased WT1 expression after in vitro incubat... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581207 Wed, 08 Jul 2009 23:12:03 +0100 2581207 http://www.medworm.com/index.php?rid=2581206&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580341%26dopt%3DAbstract Authors: Mego M, Rejlekova K, Reckova M, Sycova-Mila Z, Obertova J, Rajec J, Mardiak J Early serum tumor marker decline (STMD) during chemotherapy was shown to predict survival in patients with poor prognosis non-seminomatous germ cell tumors (GCT) in the first line. The aim of the study was to assess the prognostic value of STMD in relapsed GCT;s patients. From January 1995 to December 2007, all patients treated for GCT s with salvage therapy at the National Cancer Institute of Slovakia were identified from the tumor registry database and screened retrospectively for serum AFP and betaHCG level at the time of relapse. STMD rate was calculated for each patient and each tumor marker with an abnormal marker value at baseline and each tumor marker M (HCG or AFP) using only two values: the... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581206 Wed, 08 Jul 2009 23:12:03 +0100 2581206 http://www.medworm.com/index.php?rid=2581205&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580342%26dopt%3DAbstract We examined the lymphoblastoid cell line CD138- ARH-77, multiple myeloma CD138+ MOLP-8 cells, and the CD138+ bone marrow fraction of patients diagnosed with MM. In this experimental system, we observed that gamma-radiation and selected cytostatic drugs such as melphalan and dexamethasone did not significantly alter the nuclear radial arrangement of the 1q21 region and other relevant regions of chromosome 1. Similarly, conserved nuclear radial positioning after cytostatic treatment was observed for the c-myc, TP53, CCND1, and IgH loci. When analyzed Mcl-1, a protein encoded by a gene mapped to the 1q21 region, we found that the variant Mcl1S is highly expressed in multiple myeloma MOLP-8 cells, but not in peripheral blood lymphocytes of healthy donors or lymphoblastoid ARH-77 cells; this is... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581205 Wed, 08 Jul 2009 23:12:03 +0100 2581205 http://www.medworm.com/index.php?rid=2581204&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580343%26dopt%3DAbstract Authors: Cavarga I, Kocan P, Boor A, Belak J, Zak V, Kluchova Z, Slavik L, Tkacova R Autofluorescence bronchoscopy (AFB) has been shown to be sensitive to detect preneoplastic lesions in central lung airways system. In early stages of carcinogenesis, up-regulation of cyclooxygenase (COX)-2, Ki67 and/or increased angiogenesis may play a role by promoting the proliferation of tumoral cells and their resistance to apoptosis, as well as angiogenesis, tumor cell invasion and setting up of the metastatic process. The present study compared the expression of proliferative (COX-2, Ki67 and PCNA) and angiogenic markers (CD34 and NG2) between preneoplastic bronchial squamous dysplasia lesions and invasive squamous cell carcinoma. Biopsies obtained during AFB [preneoplastic lesions: low-grade (le... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581204 Wed, 08 Jul 2009 23:12:03 +0100 2581204 http://www.medworm.com/index.php?rid=2581203&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580344%26dopt%3DAbstract Authors: Matakova T, Sivonova M, Halasova E, Mistuna D, Dzian A, Masar J, Dobrota D The aim of present study was to summarize the results of a case-control study focused on genetic polymorphisms of selected Phase II metabolizing enzymes (GSTM1, T1, P1) and to investigate the association of these polymorphisms with the colorectal cancer risk among the Slovak population. <br />A case-control study with 183 colorectal cancer cases and 422 controls was conducted. DNA was extracted from peripheral blood leukocytes, and the polymorphisms of GSTM1, GSTT1 and GSTP1 enzymes were determined by PCR-based methods. Association between specific genotypes and the development of colorectal cancer were examined using logistic regression analysis to calculate odds ratios (OR) and 95% confidence in... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581203 Wed, 08 Jul 2009 23:12:03 +0100 2581203 http://www.medworm.com/index.php?rid=2581202&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580345%26dopt%3DAbstract Authors: Luczynski W, Kowalczuk O, Stasiak-Barmuta A, Ilendo E, Krawczuk-Rybak M, Chyczewski L In all types of leukemia both in children and adults there is a need for novel therapies that could reduce the risk of relapse after standard treatment. Acute lymphoblastic leukemia (ALL) cells are ineffective antigen presenting cells, but as shown by many authors including results from our laboratory, stimulation with CD40L restores their antigen expressing capacity. The development of T-cell therapies for leukemic patients can be based on discovery of leukemia-associated antigens (LAA) which could be recognized by the host immune system. The aim of our present study was to test the hypothesis that leukemia-derived dendritic cells maintain the expression of tumor associated antigens. Twenty ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581202 Wed, 08 Jul 2009 23:12:03 +0100 2581202 http://www.medworm.com/index.php?rid=2581201&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580346%26dopt%3DAbstract We examined the major cell types found in the blast gate by flow cytometry in newly diagnosed patients with MDS, compared them to normal BM and studied their variation according to WHO type. Two subsets defined by SSC were found both in normal BM and MDS, corresponding to myeloblasts and B-cell precursors. The number of B-cell precursors among all nucleated cells was equally low, independent of WHO type. However, the subset with an intermediate SSC, but CD117, CD13 and CD19 negative increased with the rise of myeloblasts. Concomitantly, the ratio between CD34+/CD117+/CD34-/CD117+ cells was increased. These two features are consistent with the maturation block occurring in the progression of the neoplastic clone. We conclude that the quantitative analysis of the cell types present in the BM... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581201 Wed, 08 Jul 2009 23:12:03 +0100 2581201 http://www.medworm.com/index.php?rid=2581200&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580347%26dopt%3DAbstract Authors: Naidu R, Har YC, Taib NA The C1772T, G1790A and C111A polymorphisms of Hypoxia-inducible factor-1alpha (HIF-1alpha) gene were analyzed in a hospital-based Malaysian population using PCR-RFLP method. Genomic DNA was extracted from the blood samples collected from 410 breast cancer patients and 275 normal and healthy women. We investigated the association between HIF-1alpha polymorphisms and breast cancer risk, and clinico-pathological parameters in the population. The genotype and allele frequencies of C1772T (P=0.0093 vs P=0.0024) polymorphism were significantly different between the breast cancer cases and normal subjects but similar association was not observed for G1790A (P>0.05) and C111A (P>0.05) polymorphisms, respectively. Women who were CT heterozygotes (OR=1.51;... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581200 Wed, 08 Jul 2009 23:12:03 +0100 2581200 http://www.medworm.com/index.php?rid=2581199&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580348%26dopt%3DAbstract Authors: Surazynski A, Jarzabek K, Miltyk W, Wolczynski S, Palka J The link between estrogen and metabolic developmental factors of endometrial carcinoma is well established. PPAR- gamma, (an important modulator of metabolism) and estrogen receptor belong to a family of nuclear hormone receptors that were shown to interact with each other. The interaction may affect transcriptional activity of these transcription factors. The anti-diabetic troglitazone (TGZ) is well known PPAR- gamma ligand. The effect of troglitazone-induced PPAR- gamma activation on estrogen-dependent stimulation of collagen biosynthesis was studied in the Ishikawa endometrial adenocarcinoma cell line. We have found that the presence of estrogen activity in growth medium (1nM) augmented collagen biosynthesis in the c... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581199 Wed, 08 Jul 2009 23:12:03 +0100 2581199 http://www.medworm.com/index.php?rid=2581198&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580349%26dopt%3DAbstract Authors: Berkova A, Zemanova Z, Trneny M, Schwarz J, Karban J, Cmunt E, Pavlistova L, Brezinova J, Michalova K The results of repeated interphase fluorescence in-situ hybridization (I-FISH, FISH) examination of 97 CLL patients and correlation of these findings with IgVH hypermutation status, ZAP-70 and CD38 expression are presented. The appearance of new, FISH-detectable, genomic aberrations during disease course, described as clonal evolution (CE), was observed in 26% of patients. The most frequent newly acquired cytogenetic abnormality was 13q deletion in 64% (16/25). In contrast to earlier studies, there was no correlation found between CE and either one of single negative prognostic factors (unmutated IgVH; CD38 positivity; ZAP-70 positivity). However, the combination of all three ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581198 Wed, 08 Jul 2009 23:12:03 +0100 2581198 http://www.medworm.com/index.php?rid=2581197&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19580350%26dopt%3DAbstract Authors: Brabec P, Sufliarsky J, Linke Z, Plank L, Mrhalova M, Pavlik T, Klimes D, Gregor J Due to problems with identification and an incomplete understanding on the gastrointestinal stromal tumors (GIST) before 2001, there has been a lack of comprehensive long-term population-based studies on GIST epidemiology at present date. We used data from the online registry of Czech and Slovak GIST patients (http://gist.registry.cz/), which has been compiled and maintained since 2006 and involves patients diagnosed from the year 2000. 278 patients were included in this study. Most of the tumors fell into the high-risk category (58.7%), followed by the intermediate (21.4%), low (16.6%) and very low (3.3%) categories. <br />Locations other than the small intestine and stomach had significa... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2581197 Wed, 08 Jul 2009 23:12:03 +0100 2581197 http://www.medworm.com/index.php?rid=2529573&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469651%26dopt%3DAbstract Authors: Konieczna A, Lichnovka R, Erdosova B, Ehrmann J One of the most important features of embryonic cells is their resistence to xenobiotics, which provides a natural protection for embryos against these potentially harmfull molecules. In this way, embryo cells resemble cancer cells and thus understanding the basis of this phenomenon may contribute to overcoming the multi-drug-resistance (MDR) of some tumours. Peroxisome proliferator-activated receptors (PPARs) are steroid nuclear receptors that regulate diverse biological processes such as lipid and carbohydrate metabolism, development, differentiation, apoptosis, neoplastic transformation, inflammation and regeneration of tissues. Recently it has been found that they may also regulate the expression of some MDR proteins. In this... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529573 Sat, 27 Jun 2009 13:40:03 +0100 2529573 http://www.medworm.com/index.php?rid=2529572&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469652%26dopt%3DAbstract In this study, we evaluated immunolocalization of Pan1b in human prostate cancer specimens obtained from surgery (n=40), and correlated the findings with clinicopathological features of the patients in order to study its clinical significance. Pan1b immunoreactivity was detected in 19 cases (48%) and was significantly associated with cancer of seminal vesicle invasion (P Keywords: 17ss-hydroxysteroid dehydrogenase type XI, prostate cancer, immunochemistry. PMID: 19469652 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529572 Sat, 27 Jun 2009 13:40:03 +0100 2529572 http://www.medworm.com/index.php?rid=2529570&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469653%26dopt%3DAbstract Authors: Bae HJ, Song JH, Noh JH, Kim JK, Jung KH, Eun JW, Xie HJ, Ryu JC, Ahn YM, Kim SY, Lee SH, Yoo NJ, Lee JY, Park WS, Nam SW EphA3 is acomponent of the Eph/ephrin tyrosine kinase system, which participates in vasculature development. This receptor/ligand system is associated with various signaling pathways related to cell growth and viability, cytoskeletal organization, cell migration, and anti-apoptosis. Accumulated evidence suggests that aberrant regulation of EphA3 and its genetic alterations are implicated in the development and progression of various cancers. However, despite ahigh incidence of EphA3 over-expression, no such investigation has been performed in hepatocellular carcinoma. Thus, we investigated genetic alterations of the EphA3 gene in 73 cases of hepatocellular ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529570 Sat, 27 Jun 2009 13:40:03 +0100 2529570 http://www.medworm.com/index.php?rid=2529568&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469654%26dopt%3DAbstract This study demonstrates stage-specific expression of some genes that may have potential prognostic significance.<strong><br /></strong> Keywords: gene expression profiling, myelodysplastic syndrome, CD34+ cells. PMID: 19469654 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529568 Sat, 27 Jun 2009 13:40:03 +0100 2529568 http://www.medworm.com/index.php?rid=2529566&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469655%26dopt%3DAbstract We examined the MSI, frameshift mutations and polymorphisms of the MBD4 gene in 84 patients with gastric cancers, 82 colorectal cancers and 299 healthy controls. MSI was found in 19 (22.6%) and 26 (31.7%) of the gastric and colorectal cancer samples, respectively. The mutation analysis revealed no frameshift mutations in the MBD4 gene among the gastrointestinal cancers. The frequencies of genotypes: Glu/Glu, Glu/Lys and Lys/Lys were 41.7% (35/84), 41.7% (35/84) and 16.6% (14/84), respectively, in the gastric cancer cases, and 42.7% (35/82), 36.6% (30/82) and 20.7% (17/82), respectively, in the colorectal cancers. MSI was not associated with the MBD4 codon 346 polymorphism and there was no significant difference in the frequency of the genotypes between healthy controls and gastric cancer p... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529566 Sat, 27 Jun 2009 13:40:03 +0100 2529566 http://www.medworm.com/index.php?rid=2529564&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469656%26dopt%3DAbstract Authors: Stankovic MM, Nestorovic AR, Tomovic AM, Petrovic-Stanojevic ND, Andjelic-Jelic MS, Dopudja-Pantic VB, Nagorni-Obradovic LjM, Mitic-Milikic MM, P Radojkovic D Chronic obstructive pulmonary disease (COPD) and lung cancer (LC) are amajor cause of morbidity and mortality worldwide. In both diseases airways inflammation plays an important role. Functional promoter polymorphism, at the position -308, of tumor necrosis factor (TNF)-alpha represents attractive potential susceptibilty marker for both diseases. In order to investigate the role of this polymorphism in COPD and LC, acase-control study was performed. The patient groups consisted of 97 subjects with COPD and 70 subjects with LC, while the control group encompassed 102 subjects. Results of our study showed significant decre... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529564 Sat, 27 Jun 2009 13:40:03 +0100 2529564 http://www.medworm.com/index.php?rid=2529562&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469657%26dopt%3DAbstract Authors: Bencko V, Rames J, Ondrusova M, Plesko I, Jurickova L, Trnovec T This paper describes results of two ecological studies design to analyze the incidence of selected malignancies in two populations exposed to polychlorinated hydrocarbons, mostly PCBs and TCDDs/Fs by comparing data available in the National Cancer Registry of the Slovak Republic and National Oncological Registry of the Czech Republic databases for the Slovak Republic (~ 5M inhabitants) and the Czech Republic (10,3 Minhabitants) to the data relevant for the population of Michalovce District, the Slovak Republic (~ 112,000 inhabitants) and Uherske Hradiste, the Czech Republic (146,000 inhabitants). Those districts are recognized as PCB-contaminated areas due to production and industrial use of PCBs. <br />Dat... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529562 Sat, 27 Jun 2009 13:40:03 +0100 2529562 http://www.medworm.com/index.php?rid=2529560&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469658%26dopt%3DAbstract Authors: Mlynarczyk-Liszka J, Maksymiuk B, Ponikiewska D, Krzyzowska-Gruca S, Lange D, Krawczyk Z, Malusecka E Fine needle aspiration (FNA) is routine diagnostic tool in breast tumors assessment. In some cases, however, limitations of this method do not permit an unequivocal diagnosis. In these, suspected, cases immunocytochemical evaluation of selected biological markers may be of help. The aim of the study was assessment of HSP27 value in diagnosis and discrimination of benign and malignant breast lesions. HSP27 expression was examined by immunocytochemistry in fine needle aspiration smears assessed to C2-C5 categories. In C5 subgroup HSP27 expression was correlated with ER, PR content. Statistically significant differences in HSP27 expression between subsets C2/C5 and C3/C5 were fou... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529560 Sat, 27 Jun 2009 13:40:03 +0100 2529560 http://www.medworm.com/index.php?rid=2529558&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19469659%26dopt%3DAbstract Authors: Karna E, Palka JA Collagen as aligand for integrin receptors plays important role in the integrin - dependent regulation of cellular metabolism. Since betulinic acid (BA) evokes anticancer activity, its effect on collagen biosynthesis was studied in cultured endometrial adenocarcinoma cells. Confluent cells were treated with different concentrations of BA for 24 hours. It was found that BA inhibit collagen biosynthesis ([3H] proline incorporation assay). The mechanism of this phenomenon was found at the level of insulin-like growth factor-Ireceptor (IGF-IR) and alpha2 integrin signalling (Western immunoblot analysis). The expressions of IGF-Ireceptor and alpha2 integrin subunit as well as integrin activated focal adhesion kinase (FAK) were decreased in the cells treated with B... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529558 Sat, 27 Jun 2009 13:40:03 +0100 2529558 http://www.medworm.com/index.php?rid=2529555&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19473053%26dopt%3DAbstract Authors: Hlobilkova A, Ehrmann J, Knizetova P, Krejci V, Kalita O, Kolar Z Astrocytomas, particularly high grade astrocytoma, are brain tumors with potent angiogenic activity. Our immnunohistochemical study assessed vascular endothelial growth factor (VEGF), VEGF receptors (Flk-1, and Flt-1), the intermediate filamental protein nestin which plays a role in central nervous system development, and MMP-9, which belongs the family of matrix metalloproteinases implicated in tumor invasion and angiogenesis regulation. We investigated the expression of VEGF, its receptors, nestin and MMP-9 in astrocytomas and their correlation with tumor grade. We used paraffin-embedded samples from 66 patients, 29 with low grade (WHO-grade II) and 37 with high grade (WHO-grade III and IV) astrocytomas. Antib... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529555 Sat, 27 Jun 2009 13:40:03 +0100 2529555 http://www.medworm.com/index.php?rid=2529553&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19473054%26dopt%3DAbstract Authors: Sufliarsky J, Chovanec J, Svetlovska D, Minarik T, Packan T, Kroslakova D, Lalabova R, Helpianska L, Horvathova D, Sevcik L, Spacek J, Laluha A, Tkacova V, Malec V, Rakicka G, Magdin D, Jancokova I, Dorr A, Stresko M, Habetinek V, Koza I Despite progress in primary treatment of patients with advanced ovarian cancer, the majority develop recurrence of the disease. A platinum salt treatment, either as monotherapy or in combination with another cytostatic agent, is indicated for patients who have relapsed 6 or more months after primary treatment and thus have platinum-sensitive relapse. Because repeated use of paclitaxel treatment may lead to substantial neurotoxicity, the combination of gemcitabine with carboplatin represents a suitable treatment option, which is widely used in ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529553 Sat, 27 Jun 2009 13:40:03 +0100 2529553 http://www.medworm.com/index.php?rid=2529551&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19473055%26dopt%3DAbstract Authors: Hyrsl L, Zavada J, Zavadova Z, Kawaciuk I, Vesely S, Skapa P We investigated the expression of cell-associated CAIX protein in histological sections of the transitional cell carcinoma (TCC) of the urinary tract and of the soluble form of CAIX (s-CAIX) shed by the tumor into the serum and urine of TCC patients. A total of 23 patients with histologically confirmed TCC or squamous cell carcinoma (SCC) were enrolled in the pilot study. Sixteen healthy individuals served as controls. Membrane-bound CAIX was present in the tumor cells near the endoluminal surface. Necrosis was observed in only 4 samples. Using Western blots, s-CAIX concentrated from urine was visualized as a double band at 50 and 54 kDa. In most cases, the presence of s-CAIX in the urine correlated with CAIX express... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529551 Sat, 27 Jun 2009 13:40:03 +0100 2529551 http://www.medworm.com/index.php?rid=2529549&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19473056%26dopt%3DAbstract Authors: Kleibl Z, Fidlerova J, Kleiblova P, Kormunda S, Bilek M, Bouskova K, Sevcik J, Novotny J Alterations in dihydropyrimidine dehydrogenase gene (DPYD) coding for the key enzyme (DPD) of fluoropyrimidines (FPs) catabolism contribute to the development of serious FPs-related toxicity. We performed mutation analysis of DPYD based on cDNA sequencing in 76 predominantly colorectal cancer patients treated by FPs with early development of high (grade 3-4) hematological and/or gastrointestinal toxicity. Six previously described [85T>C (C29R), 496A>G (M166V), 775A>G (K259E), 1601G>A (S534N), 1627A>G (I543V), IVS14+1G>A, 2194G>A (V732I)] and two novel [187A>G (K63E) and 1050 G>A (R357H)] non-synonymous DPYD variants were found in 56/76 (73.7%) high-toxicity patie... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529549 Sat, 27 Jun 2009 13:40:03 +0100 2529549 http://www.medworm.com/index.php?rid=2529547&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19473057%26dopt%3DAbstract Authors: Mezencev R, Kutschy P, Salayova A, Updegrove T, McDonald JF Nitrogen mustards cis-1-methoxy-2-deoxy-2-[N,N-bis(2 -chloroethyl)amino]spirobrassinol (4) and trans-1-methoxy-2-deoxy-2-[N,N-bis(2 -chloroethyl)amino]spirobrassinol (5) derived from 1-methoxyspirobrassinol, an indole phytoalexin produced by the Japanese radish Raphanus sativus var. hortensis were designed as prospective dual-action compounds with DNA-alkylating effect and glutathione-depleting effects that may sensitize cancer cells to alkylating agents. Both new compounds demonstrated cytostatic/cytotoxic effects on various leukemia and ovarian cancer cell lines and dsDNA-destabilizing effects in vitro. Compound 4, the more promising of the two compounds, exerts earlier onset of anticancer effects on Jurkat cells vi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2529547 Sat, 27 Jun 2009 13:40:03 +0100 2529547 http://www.medworm.com/index.php?rid=2303987&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309219%26dopt%3DAbstract Authors: Novotny L, Rauko P The presented review article deals with various conjugates of arabinosylcytosine (araC). This powerful drug that is routinely used in therapy of hematological malignancies has some shortcomings, which limit its use and therapeutic effects. These are low lipophilicity, low stability to degrading enzymes and need for biological activation through phosphorylation. Conjugating araC to another molecule is done with the intention of increasing araC stability and lipophilicity and possibly avoiding rate-limiting araC phosphorylation. An attachment of that another molecule, possessing its own biological activity, may result in formation of a conjugated molecule with new biological activities and better therapeutic potential. The review deals with various araC conjug... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303987 Tue, 07 Apr 2009 00:43:03 +0100 2303987 http://www.medworm.com/index.php?rid=2303985&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309220%26dopt%3DAbstract This study was local, prospective and cross-sectional. It was carried at Department of Clinical Oncology and Radiation Therapy of Charles University Hospital in Hradec Kralove, Czech Republic. Dates were obtained during year 2007 - 2008 in 64 palliative care female patients. The mean age for all 64 subjects was 60,5 years old (aged 29 - 88 years old). The Czech version of Zung self-rating depression scale was performed. </p><p align="justify">The statistical evaluation presents that mean SDS (self-rating depression score) certifies the presence of signs of mildly depression among palliative care female patients (SDS range was 50-59). The mean SDS in all subjects was 56. The mean SDS in group of healthy females was 38,9 (normal range). The incidence of depression is 71,8% (46 of... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303985 Tue, 07 Apr 2009 00:43:03 +0100 2303985 http://www.medworm.com/index.php?rid=2303983&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309221%26dopt%3DAbstract Authors: Petrackova M, Sobotkova E, Duskova M, Jinoch P, Vonka V B210 cells are murine (BALB/c) cells transformed by bcr-abl fusion gene. After intravenous administration they are capable of inducing leukaemia-like disease in syngeneic mice. From these cells a thymidine-kinase less subline was derived. It was significantly less pathogenic than the parental cells. However, a highly pathogenic clone denoted B210cTK<sup>-</sup>/cl-2 was isolated from its population. As determined by Western blotting, these cells produced more p210<sup>bcr-abl</sup> protein than the parental B210 cells. To successfully transfect these cells a modified electroporation method was introduced. Bicistronic plasmids carrying gene for herpes simplex thymidine kinase (HSV TK) and the gene f... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303983 Tue, 07 Apr 2009 00:43:03 +0100 2303983 http://www.medworm.com/index.php?rid=2303981&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309222%26dopt%3DAbstract Authors: Czyzewski K, Styczynski J An increasing resistance to imatinib is an emerging problem in patients with chronic myeloid leukemia (CML). The aim of the study was to asses mechanisms related to cellular drug resistance in imatinib-resistant derivates of chronic myeloid leukemia K-562 cell line. A parental K-562 and its imatinib-resistant derivate cell lines were used. Cell lines were tested for cytotoxicity of imatinib, cytarabine, busulfan and etoposide by the MTT assay. The cytotoxicity was expressed as IC50, inhibitory concentration for 50% of cells. Multidrug resistance proteins expression, rhodamine retention and daunorubicin accumulation were measured for each cell line. Continuous exposition of K-562 cell line to 0.01-0.02 mM imatinib resulted in development of resistance,... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303981 Tue, 07 Apr 2009 00:43:03 +0100 2303981 http://www.medworm.com/index.php?rid=2303979&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309223%26dopt%3DAbstract Authors: Duraj J, Hunakova L, Bodo J, Jakubikova J, Chovancova J, Sedlak J The aim of this study was to compare the effect of a new synthetic isothiocyanate derivative, ethyl 4-isothiocyanatobutanoate (E-4IB) and cisplatin (CDDP) in CDDP-sensitive human ovarian carcinoma cell line (A2780) and its resistant subline (A2780/CP). In parental cells, in comparison to untreated cells, sequential administration of both compounds led to higher exosomal dye (LysoTracker Green DND-26) retention and to alterations of mitogen-activated protein kinases (MAPKs), JNK, ERK and p38, or Akt kinase accompanied by changes in several anti- and pro-apoptotic molecules and lysosomal protein LAMP-1, as detected by Western blotting. On the contrary, variant A2780/CP cells were resistant to CDDP- or to combined ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303979 Tue, 07 Apr 2009 00:43:03 +0100 2303979 http://www.medworm.com/index.php?rid=2303977&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309224%26dopt%3DAbstract Authors: Rejlekova K, Mego M, Sycova-Mila Z, Obertova J, Rajec J, Salek T, Mardiak J The aim of the study was to define prognostic factors of overall and event- free survival in patients with germ cell tumors progressing after platinum-based induction chemotherapy with or without surgery. A total of 98 progressing patients were identified out of 700 patients with germ cell tumors treated with platinum-based induction chemotherapy in National Cancer Institute in Bratislava with or without surgery. 98 progressing patients received first salvage chemotherapy from October 1986 to November 2007 due to progression after a previous partial or complete response to induction chemotherapy as well as patients who failed to achieve favourable response to primary therapy. Prognostic factors of surv... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303977 Tue, 07 Apr 2009 00:43:03 +0100 2303977 http://www.medworm.com/index.php?rid=2303975&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309225%26dopt%3DAbstract In this study, we investigated the effect<sup> </sup>of the ClC inhibitor on cell proliferation, cell cycle progression in human laryngeal cancer cell line Hep-2, as well as the effect on the phosphorylation levels of ERK1/2 and AKT1. In this study crystal violet method was used to study the effect<sup> </sup>of the ClC inhibitor, 5-nitro-2-(3-phenylpropylamino) benzoic acid, NPPB, on Hep-2 cell proliferation. The impaction of the inhibitor on the cell cycle distribution was investigated by the flow cytometry (FCM). Western blot was performed to measure the phosphorylation levels of ERK1/2 and AKT1. Our data indicated ClC played an important role in Hep-2 cell proliferation and cell cycle. NPPB inhibited Hep-2 cell proliferation when compared with the controls. Bloc... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303975 Tue, 07 Apr 2009 00:43:03 +0100 2303975 http://www.medworm.com/index.php?rid=2303972&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309226%26dopt%3DAbstract Authors: Weismann P, Weismanova E, Masak L, Mlada K, Keder D, Ferancikova Z, Vizvaryova M, Konecny M, Zavodna K, Kausitz J, Benuska J, Repiska V The aim of this study was to establish the sensitive, specific and clinically acceptable method for detection of tumor cells (TCs) circulating in peripheral blood (PB) of cervical cancer patients without the clinically detectable risk of disease progression. <p align="justify">The 7.5 ml of PB of healthy donor was spiked with 5 to 100 cells from SiHa or HeLa cell lines. The spiked tumor cells were collected without gradient centrifugation, by standard gradient centrifugation or by modified gradient centrifugation combined with immunomagnetic separation using EpCAM antibody with affinity for epithelial cell adhesion molecule. The number o... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303972 Tue, 07 Apr 2009 00:43:03 +0100 2303972 http://www.medworm.com/index.php?rid=2303970&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309227%26dopt%3DAbstract Authors: Vesely K, Jurajda M, Nenutil R, Vesela M Soft tissue sarcomas (STSs) are rare heterogeneous tumors with variable clinical course and outcome. The management of STSs depends upon the accurate histopathological diagnosis and assessing their histological grade. Currently, core needle biopsies are becoming increasingly popular for diagnosing STSs but value of histological grading is limited from this type of specimens. To evaluate the immunohistochemical expression of p53, mdm-2, cyclin D1, p16, nm23, EGFR and Ki-67 labelling index in adult STSs patients and their association with histological grade of STSs, we analysed 101 primary untreated STSs of the limbs and trunk using the tissue microarray technique on formalin-fixed, paraffin-embedded tissue samples. The cases consisted of... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303970 Tue, 07 Apr 2009 00:43:03 +0100 2303970 http://www.medworm.com/index.php?rid=2303967&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309228%26dopt%3DAbstract In conclusion, flow cytofluorometry using anti-cytokeratin and anti-EMA antibodies may be useful in evaluating microdeposits of esophageal squamous cells in bone marrow. A dysfunctioning erythropoietic system causing anemia can be a first signal for the presence of malignant cell microdeposits in the marrow of patients with esophageal carcinoma. Keywords: epithelial cells, bone marrow, esophageal carcinoma. PMID: 19309228 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303967 Tue, 07 Apr 2009 00:43:03 +0100 2303967 http://www.medworm.com/index.php?rid=2303961&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309229%26dopt%3DAbstract Authors: Orendas P, Kassayova M, Kajo K, Ahlers I, Kubatka P, Bojkova B, Pec M, Ahlersova E The present experiment aims to evaluate tumor suppressive effects of a selective inhibitor of cyclooxygenase-2 (COX-2) celecoxib (Celebrex, Pfizer) administered alone and in combination with melatonin in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in Sprague-Dawley female rats. Celecoxib was administered daily at a concentration of 1.666 g/kg diet to two groups during 20 weeks (starting a week before first NMU application). A combination of celecoxib and melatonin applied in drinking water (20 microg/ml drinking water), daily from 15:00 to 08:00 hours was administered to the second group. The anticarcinogenic effects of chemopreventive drugs were compared with c... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303961 Tue, 07 Apr 2009 00:43:03 +0100 2303961 http://www.medworm.com/index.php?rid=2303958&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309230%26dopt%3DAbstract Authors: Kumar S, Deepak P, Acharya A Heat Shock proteins 70 (Hsp70) is a family of highly conserved molecules that maintain the function of crucial cellular pathways during stress. Hsp70 derived from tumor cells is bound with tumor antigenic peptides from the diverse antigen cytosolic pool. Tumor-derived Hsp70 preparations after <em>ex vivo</em> administration permit antigen presenting cells (APCs) to present tumor antigen to their cell surface and induce tumor specific immunity in many types of malignancies by directly eliciting cytotoxic T lymphocytes (CTL) response. In the present investigation, we have demonstrated that immunization with tumor cell derived Hsp70 lead to an effective survival advantage in mice with minimal residual tumor cells from which Hsp70 is derive... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303958 Tue, 07 Apr 2009 00:43:03 +0100 2303958 http://www.medworm.com/index.php?rid=2303952&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309231%26dopt%3DAbstract Authors: Bojkova B, Orendas P, Garajova M, Kassayova M, Kutna V, Ahlersova E, Ahlers I In this paper the chemopreventive effect of peroral antidiabetic metformin in mammary carcinogenesis in female Sprague-Dawley rats was evaluated. Mammary carcinogenesis was induced by N-methyl-N-nitrosourea (NMU) administered in two intraperitoneal doses each per 50 mg/kg b.w. between 43.-55. postnatal days. Metformin was administered in drinking water (at a concentration of 50 microg/ml and 500 microg/ml) 13 days before the first NMU dose until the termination of the experiment. During the experiment the animals were weekly weighed and palpated for the presence of mammary tumors, the incidence, latency, tumor frequency, and tumor volume were recorded. The experiment was terminated 18 weeks after the... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303952 Tue, 07 Apr 2009 00:43:03 +0100 2303952 http://www.medworm.com/index.php?rid=2303950&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19309232%26dopt%3DAbstract Authors: Zavodna K, Konecny M, Krivulcik T, Spanik S, Behulova R, Vizvaryova M, Weismanova E, Galbavy S, Kausitz J Colorectal carcinoma (CRC) represents a serious problem worldwide: in the Slovak republic are diagnosed about 2600 new CRC cases annually and its incidence is increasing. Colorectal cancer patients may succumb to the disease because of local recurrence or local formation of metastasis. Therefore, it is necessary to modulate therapeutic algorithm with new methods, leading to early diagnostic of CRC or changing the existing therapeutic procedures. <p align="justify">Recent progresses have been made in understanding of EGFR pathway involved in CRC carcinogenesis, especially the role of Ras protein. Mutations in <em>KRAS</em> oncogene are frequently found in ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2303950 Tue, 07 Apr 2009 00:43:03 +0100 2303950 http://www.medworm.com/index.php?rid=2217191&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239320%26dopt%3DAbstract Authors: Cholujova D, Jakubikova J, Sedlak J BioBran, enzymatically modified arabinoxylan from rice bran was tested for its possible effects on in vitro maturation of human dendritic cells (DC). Immature DC (iDC) derived from plastic-adhered, IL-4 and GM-CSF treated peripheral monocytes (Mo) were further cultured with cytokine maturation mix 1 (CMM1; TNF-alpha, IL-1beta and IL-6) or CMM2 (LPS and IFN-gamma) to induce their maturation into mature DC (matDC1 or matDC2, respectively). Different concentrations of BioBran (10, 100, 400 and 1000 microg/ml) were applied in the presence or absence of relevant CMM to assess the effects of BioBran on DC maturation processes. BioBran induced maturation of iDC, as these cells cultured with IL-4/GM-CSF/BioBran down-regulated CD14 and CD1a antigens ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217191 Thu, 26 Feb 2009 21:21:31 +0100 2217191 http://www.medworm.com/index.php?rid=2217190&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239321%26dopt%3DAbstract Authors: Garley M, Jablonska E, Grabowska SZ, Piotrowski L Epithelial squamous cell carcinoma is the predominant histological type among cancers of the head and neck. It is characterised by high growth dynamics and a low survival rate of patients. The usefulness of various markers and prognostic factors is assessed to improve treatment results in patients with epithelial squamous cell carcinoma. <p align="justify">The involvement of neutrophils (PMNs) in the neoplastic process and scarce evidence for the role of interleukin 17 (IL-17) family cytokines in these reactions encouraged us to undertake a study in this field. We assessed the expression and capability of neutrophils in patients with oral epithelial squamous cell carcinoma<em> </em>to release IL-17E and IL-17A... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217190 Thu, 26 Feb 2009 21:21:31 +0100 2217190 http://www.medworm.com/index.php?rid=2217189&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239322%26dopt%3DAbstract Authors: Krivulcik T, Sedlak J, Bartosova Z The human multidrug resistance gene (<em>MDR1</em>) is encoding the transmembrane transporter P-glycoprotein (P-gp) which plays an important role in the efflux of various drugs and thus is potentially influencing the drug-treatment outcome. It has been indicated that the level of P-gp activity may be affected by the presence of single nucleotide polymorphisms (SNP) in the gene which led to the studies estimating <em>MDR1</em>-SNP frequencies in various populations. Here, we have investigated the occurrence of seven SNP in the <em>MDR1</em> gene for the first time in Slovak population using multiplex SNaPshot genotyping method. The allelic frequencies of the most common gene variants, i.e. 1236C>T, 2677G&... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217189 Thu, 26 Feb 2009 21:21:31 +0100 2217189 http://www.medworm.com/index.php?rid=2217188&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239323%26dopt%3DAbstract We report here that beta-arrestin2 significantly blocks morphine-induced number of cell death in human breast cancer MCF-7 and MDA-MB231 cells. Suppression of endogenous beta-arrestin2 by specific RNA interfering (RNAi) and morphine treatment significantly attenuates the levels of phosphorylated Akt compared with inhibition of beta-arrestin2 or morphine treatment alone. However, blockade of morphine-induced cell death by beta-arrestin2 seems to be dependent on the inhibition of caspase-8, as inhibition of beta-arrestin2 and morphine treatment significantly enhanced the levels of cleaved caspase-8. These studies show for the first time that beta-arrestin2 blocks morphine-induced cell death through anti-apoptotic Akt and pro-apoptotic caspase-8 pathways. Therefore, targeting beta-arrestin2 m... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217188 Thu, 26 Feb 2009 21:21:31 +0100 2217188 http://www.medworm.com/index.php?rid=2217187&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239324%26dopt%3DAbstract Authors: Cao Z, Song JH, Park YK, Maeng EJ, Nam SW, Lee JY, Park WS TP53 is a major gene involved in the determination of proliferation or growth arrest at the cellular level. The polymorphism of p53 at codon 72 has been widely studied; this variation has been associated with cancer susceptibility and disease outcome. The specific aim of this study was to investigate whether the p53 codon 72 polymorphism is associated with individual susceptibility to colorectal cancer in Korean patients. The frequency of the polymorphism was examined in 156 patients with colorectal cancer and in 293 healthy controls. The polymorphism analysis was performed by amplifying exon 4 of p53 and digesting the products with restriction enzyme. The frequencies of genotypes: Arg/Arg, Arg/Pro and Pro/Pro were 34.... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217187 Thu, 26 Feb 2009 21:21:31 +0100 2217187 http://www.medworm.com/index.php?rid=2217186&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239325%26dopt%3DAbstract Authors: Park JK, Song JH, He TC, Nam SW, Lee JY, Park WS The binding of the Wnt ligand to its receptor Frizzled, activates the Wnt canonical signaling pathway in carcinogenesis as well as many cellular processes, including cellular proliferation and differentiation. Wnt-2, one of 19 members of the <em>Wnt</em> gene family, is frequently overexpressed in malignant tissues. Here, in order to investigate the role of Wnt-2 in colorectal carcinogenesis, we examined the expression of the Wnt-2 protein in 120 colorectal cancers by immunohistochemistry. Wnt-2 protein was expressed in the cell membrane and cytoplasm and up-regulated in 74 (61.7%) of 120 colorectal cancers. Statistically, overexpression of Wnt-2 protein was not associated with the clinical and pathological parameter... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217186 Thu, 26 Feb 2009 21:21:31 +0100 2217186 http://www.medworm.com/index.php?rid=2217185&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239326%26dopt%3DAbstract Authors: Sadlonova V, Kubatka P, Kajo K, Ostatnikova D, Nosalova G, Adamicova K, Sadlonova J The aim of this study was to assess side effects of aromatase inhibitor anastrozole in the prevention of N-methyl-N-nitrosourea - induced pre-menopausal mammary carcinogenesis in female Sprague-Dawley rats. This model mimicked situation in healthy, but from the point of view of the development of breast cancer, high-risk pre-menopausal women. <p align="justify">Aromatase inhibitor anastrozole was used as a chemopreventive agent taken by the animals in the food during the whole period of time of the experiment. Group 1 - the control group had taken food without anastrozole, the groups 2 and 3 with anastrozole in various concentrations - 0.05 mg/1 kg of food (ANA 0.05) and 0.5 mg/1 kg of fo... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217185 Thu, 26 Feb 2009 21:21:31 +0100 2217185 http://www.medworm.com/index.php?rid=2217184&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239327%26dopt%3DAbstract Authors: Adamkov M, Lauko L, Balentova S, Pec J, Pec M, Rajcani J The anti-apoptotic protein survivin was detected in a panel of 27 dysplastic nevi. From each representative paraffin block 4 mm sections were cut and stained with anti-survivin antibody (DAKO, Clone 12C4). In each section, the labeling intensity, the subcellular location of survivin antigen, the percentage of labeled cells and the degree of dysplasia were assessed. Survivin was present in 23 out of 27 cases (85.2%), but absent in 4/27 cases (14.8%). Positive staining was confined to the cytoplasm (C) of nevus cells only in 18 cases (66.7%), while cytoplasmic as well as nuclear positivity (NC) was found in 5 cases (18.5%). In no case solely nuclear staining could be seen. Furthermore, in 4 out of 5 cases (80%) with NC sta... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217184 Thu, 26 Feb 2009 21:21:31 +0100 2217184 http://www.medworm.com/index.php?rid=2217183&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239328%26dopt%3DAbstract Authors: Xie HJ, Bae HJ, Noh JH, Eun JW, Kim JK, Jung KH, Ryu JC, Ahn YM, Kim SY, Lee SH, Yoo NJ, Lee JY, Park WS, Nam SW The <em>Janus kinase 1</em> (JAK1) gene encodes a cytoplasmic tyrosine kinase that is noncovalently associated with a variety of cytokine receptors and plays a nonredundant role in cell proliferation, survival, and differentiation. The mutated forms of JAK1 often altered the activation of JAK1 and then changed the activation of JAK1/STAT pathways, and this may contribute to cancer development and progression. Thus, to investigate whether genetic mutations of JAK1 gene are associated in hepatocellular carcinoma (HCC) progression, we analyzed genetic alterations of JAK1 gene in 84 human HCCs by single-strand conformational polymorphism (SSCP) and direct se... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217183 Thu, 26 Feb 2009 21:21:31 +0100 2217183 http://www.medworm.com/index.php?rid=2217182&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239329%26dopt%3DAbstract This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. <p align="justify">Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-<em>trans</em> retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in the... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217182 Thu, 26 Feb 2009 21:21:31 +0100 2217182 http://www.medworm.com/index.php?rid=2217181&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239330%26dopt%3DAbstract Authors: Oblak I, Petric P, Anderluh F, Velenik V, Hudej R, Fras AP Results of radiochemotherapy in 50 patients with squamous cell carcinoma of the anal canal, treated with radical radiochemotherapy between January 2003 and September 2007, at the Institute of Oncology Ljubljana are presented. The treatment schedule consisted of 3-D conformal external beam radiotherapy (45 Gy in 25 fractions), with two cycles of concurrent chemotherapy (5-fluorouracil (5-FU) / Mitomycin C), followed by brachytherapy or external beam boost (15-30 Gy) to the primary tumor. Locoregional control (LRC), disease-free survival (DFS), disease-specific survival (DSS), overall survival (OS) and colostomy-free survival (CFS) rates and the rate of acute and chronic side-effects were estimated. The impact of individ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217181 Thu, 26 Feb 2009 21:21:31 +0100 2217181 http://www.medworm.com/index.php?rid=2217180&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239331%26dopt%3DAbstract Authors: Chorvath M, Boljesikova E, Pruzincova L, Procka V, Rychly B, Novotny M, Kalina P, Belan V, Makaiova I, Steno J Presented is the analysis of patients who underwent external beam radiotherapy (EBRT) to the brain in the period from 2003 to 2006 at the department of Radiation Oncology of the St. Elisabeth Cancer Institute. <p align="justify">The aim of our analysis was to identify risk factors of late delayed therapy induced injuries (LDTI) in the brain. The patients were regularly examined with magnetic resonance (MRI), including conventional and advanced techniques: perfusion imaging (pMRI), diffusion weighted imaging (DWI), MRI spectroscopy (MRS). The results from MRI were correlated with <sup>18</sup>fluoro-deoxyglucose positron emission tomography (<sup&g... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217180 Thu, 26 Feb 2009 21:21:31 +0100 2217180 http://www.medworm.com/index.php?rid=2217179&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239332%26dopt%3DAbstract Authors: Petera J, Matula P, Paluska P, Sirak I, Macingova Z, Kasaova L, Frgala T, Hodek M, Vosmik M Low dose rate (LDR) brachytherapy is a well established treatment for the early stages of tongue cancer. High dose rate (HDR) afterloading devices have replaced LDR brachytherapy in many radiotherapy departments, but the effect and safety of HDR brachytherapy in comparison with LDR brachytherapy for interstitial applications is an unresolved question. The aim of our radiobiological study was to utilize dose volume histiograms from patients treated in our institution to simulate the risk of complication of LDR and HDR brachytherapy. Normal tissue complication probabilities (NTCP) of acute mucositis, late mucosal necrosis and osteoradionecrosis of two HDR brachytherapy schedules (18 x 3 G... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217179 Thu, 26 Feb 2009 21:21:31 +0100 2217179 http://www.medworm.com/index.php?rid=2217178&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19239333%26dopt%3DAbstract Authors: Chen Y, Kong Q Abnormal centrosome frequently found in human cancer is a major cause of mitotic defects and chromosome instability in cancer cells. Centrosome duplication is controlled in a cell cycle-specific manner, whereas cancer cells with dysregulation of centrosome duplication can survive and reenter the cell cycle through defective cell cycle checkpoint systems. Although numerous studies showed that centrosome amplification can be readily induced by loss or mutational inactivation of p53, however, the role of centrosomally localized p53 in the regulation of centrosome duplication had been enigma. To investigate the role of centrosome and p53 in the in vivo carcinogenesis, we performed immunofluorescence and Western blot analysis, respectively, to detect the alteration o... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2217178 Thu, 26 Feb 2009 21:21:31 +0100 2217178 http://www.medworm.com/index.php?rid=2116338&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152238%26dopt%3DAbstract Authors: V AM, D SS, Z KV, M PN, V OB Immunoscintigraphy combines the advances in immunology and nuclear medicine to target tumor sites. Visualization of colorectal carcinomas is based on different monoclonal antibodies and their fragments against tumor-associated antigens labeled with gamma emitting radionuclides which accumulate in the tumor tissue due to their interaction with corresponding antigens. Available data on the role of immunoscintigraphy in detection of recurrence and metastases of colorectal carcinomas are reviewed. Despite a variety of investigations related to the application of immunoscintigraphy in diagnostics of colorectal cancer, using different radiolabeled immunoreactive agents and imaging methods there has not been a consensus among the investigators regarding t... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116338 Wed, 21 Jan 2009 04:58:05 +0100 2116338 http://www.medworm.com/index.php?rid=2116337&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152239%26dopt%3DAbstract Authors: T E, J R, K S, R P, I H, J R The aim of this study was to investigate the frequency of blood count, lymphocyte subpopulations, and immunoglobulin levels alterations in a group of healthy nephroblastoma long-term survivors. The group included 122 nephroblastoma longterm survivors who were at least five years post anticancer therapy and free of any sign of recurrence The proportion of lymphocyte subpopulations was analyzed by flow cytometry using antibodies anti CD45 FITC/CD14 PE, anti CD3 FITC/ CD16+CD56 PE, anti CD4 FITC/ CD8 PE and anti CD20 FITC. Immunoglobulin G, A, and M levels were evaluated by immunoturbidimetry. Total blood count was also examined. The occurrence of decreased immunoglobulin levels, leukocytes, lymphocytes, and granulocytes count, proportion of T lymphoc... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116337 Wed, 21 Jan 2009 04:58:05 +0100 2116337 http://www.medworm.com/index.php?rid=2116336&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152240%26dopt%3DAbstract We examined the efficacy of hEGCs through coculturing SKOV3 with hEGCs, detecting tunel apoptosis and caspase-9 activity by immunocytochemistry, and measuring the expression of AKT by real-time PCR and western blot. Further a xenograft model in SCID mouse to examine the effect of hEGCs on SKOV3 in vivo was used. Our results showed that there was a 1.5-fold growth reduction for SKOV3 in the coculture group. HEGCs induced apoptosis via caspase-9 activation and AKT downregulation in SKOV3. This tumor cell inhibition was demonstrated also in the used animal tumor model. Taken together, our observations demonstrated that the hEGCs could inhibit the growth of SKOV3 by inducing apoptosis by inhibiting AKT pathway. Key words: embryonic germ cells; ovarian cancer; apoptosis. PMID: 19152240 [Pub... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116336 Wed, 21 Jan 2009 04:58:05 +0100 2116336 http://www.medworm.com/index.php?rid=2116335&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152241%26dopt%3DAbstract Authors: T KA, M R, A GF, M D, A TR, Z M, A G Interleukin-18 [IL-18] gene promoter polymorphism is reported to be a genetic risk factor for several types of cancer. The aims of this investigation were to evaluate and compare the frequencies of IL-18 gene promoter polymorphisms at positions -137 [G/C] and -607 [C/A] in breast cancer patients and healthy controls as well as to study the contribution of these data with clinicopathological parameters at diagnosis. The studied populations comprised 250 cases with breast carcinoma and 206 healthy subjects. IL-18 gene promoter polymorphisms at positions -137 and -607 were amplified in patient and control groups using allele specific polymerase chain reaction [AS-PCR]. The frequencies of GG, GC and CC genotypes of -137 SNP were 141 [56.4%], 96... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116335 Wed, 21 Jan 2009 04:58:05 +0100 2116335 http://www.medworm.com/index.php?rid=2116334&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152242%26dopt%3DAbstract In this study we investigated 153 subjects (86 M/67 F); 51 patients with newly-diagnosed PC (31 M/20 F), 45 patients with 2TDM (29 M/16 F) and 57 healthy control subjects (26 M/31 F). The polymorphism of -23HphI (A/T) in the promoter of INS was determined by the combination of polymerase chain reaction (PCR) with the restriction fragment length polymorphism (RFLP) methods. The results obtained by the PCR-RFLP analyses of SNP -23HphI were confirmed by a direct studied locus sequencing of the genomic DNA. The frequency of abnormal glucose metabolism (both DM and impaired fasting glucose) was 88 % (45/51) in PC group. The AA genotype in SNP -23HphI was more prevalent (67 % vs. 47 %; P<0.05) among PC patients compared to controls. Additionally, statistically significant differences were fou... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116334 Wed, 21 Jan 2009 04:58:05 +0100 2116334 http://www.medworm.com/index.php?rid=2116333&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152243%26dopt%3DAbstract Authors: I KJ, S KJ, Y KS, T LH, Y JJ Background of the present study was to assess the usefulness of double phase positron emission tomography/computed tomography (PET/CT) to differentiate malignant from benign pulmonary lesions with low fluorine-18 fluorodeoxyglucose (F-18 FDG) uptake. Of 218 consecutive patients who underwent double phase F-18 FDG PET/CT to evaluate pulmonary lesions found on CT, we retrospectively analyzed 30 who had focal pulmonary lesions with an SUV of <2.5. All patients underwent PET/CT of the thorax at two time points: scan 1 at 60 min and scan 2 at 120 min after the intravenous injection of 2.5 MBq F-18 FDG. The F-18 FDG PET/CT images were analyzed visually and quantitatively. Of 30 evaluated nodules, 13 (43%) proved to be malignant and 17 (57%) benign. Th... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116333 Wed, 21 Jan 2009 04:58:05 +0100 2116333 http://www.medworm.com/index.php?rid=2116332&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152244%26dopt%3DAbstract Authors: M SH, R SN, S TD, G PH, S SN, P PS The present study aimed to determine significance of E-cadherin, a cell adhesion molecule, and sialyl Lewis-X (sLeX), a cell surface antigen, in oral carcinogenesis. Expressions of E-cadherin and sLeX were detected using western blot analysis from oral malignant (n=25), and oral precancerous tissues (OPC, n=20) and their adjacent normal tissues. An altered expression of E-cadherin (E-cad) and sLeX was observed in malignant and precancerous tissues. E-cad western blot revealed presence of two bands, a 120 kDa (native, E-cad120) and a 97 kDa (known as truncated E-cad97). The accumulation of truncated E-cad97 and sLeX in malignant and OPC tissues compared to their adjacent normal tissues was observed. Receiver's Operating Characteristics (ROC) c... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116332 Wed, 21 Jan 2009 04:58:05 +0100 2116332 http://www.medworm.com/index.php?rid=2116331&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152245%26dopt%3DAbstract In this study, gastric lesions and normal epithelial cells were isolated from formalin-fixed paraffin embedded tissues using a laser capture microdissection (LCM) system, the clonality was analyzed with human androgen receptor gene (HUMARA) polymerase chain reaction (PCR), and the PCR products were examined using Applied Biosystems 3730 DNA Analyzer. The relationship between the clonal status and Ki-67 protein expression was also investigated. Ki-67 was detected by two-step immunohistochemical staining. 5/32 intestinal metaplasia lesions, 10/45 low grade intraepithelial neoplasia, 25/36 high grade intraepithelial neoplasia and 20/20 intestinal gastric carcinoma were of monoclonal origin. Similar to monoclonal inactivation, the expression rate of Ki-67 also increased along the multi-step ga... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116331 Wed, 21 Jan 2009 04:58:05 +0100 2116331 http://www.medworm.com/index.php?rid=2116330&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152246%26dopt%3DAbstract Authors: R TW, A G, B S, H RM, A K, E MP Several biochemical pathways can lead to cancer cachexia, one of which involves the elevation of the cytokines, such as tumor necrosis factor alpha (TNF-alpha) and interferon gamma (INF-gamma). It was suggested that TNF-alpha and INF-gamma genes polymorphisms may influence these cytokines serum levels, but published data are still controversial. The aim of our study was to assess the clinical significance of -308G/A TNF-alpha and +874A/T INF-gamma genes polymorphisms as well as TNF-alpha and INF-gamma serum levels in pancreatic adenocarcinoma (PA) and chronic pancreatitis (CP) as regards to healthy volunteers. We studied 41 patients with pancreatic adenocarcinoma, 56 with chronic pancreatitis and 50 healthy volunteers. Peripheral venous blood sa... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116330 Wed, 21 Jan 2009 04:58:05 +0100 2116330 http://www.medworm.com/index.php?rid=2116329&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152247%26dopt%3DAbstract Authors: D AS, E T, H O, G S, A K Abnormal regulation of the cell cycle is a feature of many neoplasms. The role of cell cycle regulators in oncogenesis has been investigated in many human tumors. Alteration of the retinoblastoma (pRb) and cyclin D1 disrupt the Rb pathway and occur in many carcinomas. However the expression of the Rb and cyclin D1 in intestinal type gastric carcinoma is unclear. The purpose of this study was to investigate the expression of Rb and cyclinD1 in resected gastric carcinoma, their adjacent nonneoplastic mucosa and normal gastric mucosa, and finally to provide insights into the role of the Rb and cyclin D1 in gastric carcinogenesis. We investigated Rb and cyclin D1 expression in 43 patients (32 men, 11 women; mean age: 64) with primary gastric adenocarcinoma... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116329 Wed, 21 Jan 2009 04:58:05 +0100 2116329 http://www.medworm.com/index.php?rid=2116328&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152248%26dopt%3DAbstract Authors: S BK, R M, P BS The present investigation was a lectin-based diagnosis of malignant prostate cancer (PC) by the interaction of phytohemagglutinin (PHA lectin) from Phaseolus vulgaris with the glycan part of serum prostate specific antigen (PSA) of patients with prostatic disorder. This was confirmed by the interaction between PHA and purified PSA obtained from serum by electrophoretic separation and finally by HPLC chromatography. The precipitate of carbohydrate content after binding of PHA with purified PSA of PC was significantly higher than that of benign prostate hyperplasia (BPH) and/or normal serum PSA. The results suggest that there may be a striking difference in glycosylation pattern of PSA between BPH and PC. The cut off value >/= 10 mug/ml of the carbohydrate con... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116328 Wed, 21 Jan 2009 04:58:05 +0100 2116328 http://www.medworm.com/index.php?rid=2116327&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152249%26dopt%3DAbstract In conclusion, this study showed that patients with 2 or 3 predictive factors had a high risk of the NAC involvement. These risk factors should be taken into consideration in patient selection for skin-sparing mastectomy with the NAC preservation. Key words: nipple-areola complex; involvement; breast cancer; mastectomy. PMID: 19152249 [PubMed - as supplied by publisher] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116327 Wed, 21 Jan 2009 04:58:05 +0100 2116327 http://www.medworm.com/index.php?rid=2116326&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152250%26dopt%3DAbstract Authors: V V, K B, A V, E F, J M, P Z, M T We processed data of 79 patients (pts) with malignant lymphoma from the National Registry of haematopoietic stem cell transplants conducted between 1997 and 2006. The haematopoietic stem cell donor in 48 pts was an HLA matched relative, and in 30 pts an unrelated volunteer. Sixty (77%) pts were transplanted with reduced intensity conditioning (RIC), eleven (23%) pts with myeloablative conditioning (MC). Acute graft-versus-host disease (aGVHD) was recorded in 26 (33%) pts. Chronic GVHD was diagnosed in 19 (36%) of the 53 assessable pts. Transplant-related mortality (TRM) in the first 100 days, 1 year and 3 years for the whole group was 26%, 33% and 33%. Twenty (26%) of the pts relapsed. During the median follow-up of 26 months the overall survi... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116326 Wed, 21 Jan 2009 04:58:05 +0100 2116326 http://www.medworm.com/index.php?rid=2116325&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19152251%26dopt%3DAbstract Authors: S R, V M, V M, T C, P R, M B, R T Presented is a retrospective analysis of 27 patients with plasma cell neoplasms of the spine treated by surgery. Multiple myeloma was confirmed in 22 (81%) and solitary plasmacytoma in 5 patients (19%), assessed at the time of surgery. Nineteen patients (70%) with the preliminary diagnosis of malignancy of unknown etiology were admitted for surgery. In 23 patients (85%) the essential symptom was back pain, which preceded surgery by an average of 4 months. Thirteen patients (48%) were bedridden due to tumor spinal cord compression, on average for 7 days before undergoing surgery. Only 5 out of 13 bedridden patients (38%) regained the ability to walk after surgery and 8 patients (62%) remained bedridden despite successful surgical decompression ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=2116325 Wed, 21 Jan 2009 04:58:05 +0100 2116325 http://www.medworm.com/index.php?rid=1955621&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999873%26dopt%3DAbstract Authors: Gerykova-Bujalkova M, Krivulcik T, Bartosova Z Germline defects in the DNA mismatch repair genes MLH1 and MSH2 are the major cause of hereditary nonpolyposis colon cancer (HNPCC), also called Lynch syndrome. Detection of inherited pathogenic change in their DNA sequence in HNPCC families allows for identification of asymptomatic individuals who require appropriate medical surveillance. However, evaluation of clinical significance of identified DNA alteration is not always straight-forward and some changes maybe classified incorrectly depending on the method used. The aim of this review is to summarize rationale, practice and pitfalls in the characterization of substitutions localized in the exons and outline new experimental and in silico approaches used to determine mutation ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955621 Thu, 13 Nov 2008 15:13:24 +0100 1955621 http://www.medworm.com/index.php?rid=1955620&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999874%26dopt%3DAbstract Authors: Wu XZ Hepatocellular carcinoma (HCC) is a hypervascular tumor, and tumor progression and prognosis is associated with angiogenesis. Extracellular matrix remodeling and inflammation play important roles in hepatocarcinogenesis. Some ingredients of extracellular matrix such as endostatin and sulfated polysaccharide, some immunomodulatory agents and cox-2 inhibitor suppress the angiogenesis of HCC. Because vasculogenic mimicry is associated with high tumor grade, some differentiation agents are used to inhibit antiagiogenesis. Besides suppressing the proliferation directly, somatostatin inhibits angiogenesis to suppress growth indirectly. Copper chelator prevents copper from functioning as a cofactor in angiogenesis. The renin-angiotensin system is frequently activated in patient... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955620 Thu, 13 Nov 2008 15:13:24 +0100 1955620 http://www.medworm.com/index.php?rid=1955619&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999875%26dopt%3DAbstract Authors: Ehrmann J, Strakova N, Vrzalikova K, Hezova R, Kolar Z Proteins of STAT family belongs to the transcription factors. Through their binding to the DNA specific sites and consequent regulation of transcription of various genes, these signaling proteins play an important role in many cell functions. Recent studies demonstrated persistent activation of STATs and loss of their natural inhibitors SOCS and PIAS in various human cancers. There is also evidence that experimental pharmacologic or genetic modulation of their function mignt by a new approach in anticancer treatment. The aim of this study was in vitro assesment and analysis of expression of STATs, SOCS and PIAS in glioblastoma cell lines undergoing treatment by PPARgamma agonists/antagonists because PPARgamma and STATs are... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955619 Thu, 13 Nov 2008 15:13:24 +0100 1955619 http://www.medworm.com/index.php?rid=1955618&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999876%26dopt%3DAbstract Authors: Lakota J, Skultety L, Dubrovcakova M, Altaner C Here we report patients with Hodgkin's disease and multiple myeloma, who relapsed/progressed after high dose therapy and autologous stem cell transplantation. In patients who developed aplastic anemia type syndrome, spontaneous tumor regression was observed and concomitantly high titers of serum autoantibodies were found. In order to identify the antibody specificity, two-dimensional electrophoresis, blotting and immunoreactions were used to analyze the peripheral blood stem cell extract with autoantibodies containing serum. The unique protein spot visualized exclusively by serum of patients with aplastic anemia type syndrome was identified as carbonic anhydrase I (CA I, accession No. P00915 and Q7M316) by means of mass spectrome... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955618 Thu, 13 Nov 2008 15:13:24 +0100 1955618 http://www.medworm.com/index.php?rid=1955617&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999877%26dopt%3DAbstract Authors: Rydzanicz M, Giefing M, Ziolkowski A, Kasprzyk M, Gabriel A, Dyszkiewicz W, Szyfter K Lung cancer is one of the most common malignancies and cancer-related death worldwide. Lymph node metastasis is the main cause of treatment failure. Although many studies were performed to evaluate genetic events associated with development and progression of lung cancer, molecular mechanism still remains poorly defined. In the present study, using comparative genomic hybridization (CGH) technique, we described the pattern of DNA copy number changes in a cohort of 42 primary squamous cell carcinomas (SCC) of the lung. A direct comparison of nonmetastatic (TxN0M0) and metastatic (TxN1-2M0) tumors was performed to define chromosomal imbalances related to lymph node metastases. Some genetic alte... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955617 Thu, 13 Nov 2008 15:13:24 +0100 1955617 http://www.medworm.com/index.php?rid=1955616&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999878%26dopt%3DAbstract Authors: Babusikova O, Zeleznikova T The cellular diversity of bone marrow samples was studied by using multi-dimensional cluster analysis of six-parametric flow cytometry data (four CD, forward scatter and side scatter), focusing mainly on acute leukemia blast cells and regeneration of normal B-cells, hematogones. This approach should enhance the ability to study normal hematopoiesis, and to identify and monitor hematopoietic disorders. The study was performed on a homogeneous group of patients (mainly children), all of them after finishing complete therapy for AL, mostly B-ALL. In all of these patients complete pattern of all three individual Hg stages was present. Maturation spectra of surface immunoglobulin kappa (sIgkappa) and lambda (sIglambda) light chains and IgM, IgA heavy cha... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955616 Thu, 13 Nov 2008 15:13:24 +0100 1955616 http://www.medworm.com/index.php?rid=1955615&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999879%26dopt%3DAbstract In conclusion, these studies enabled us visualized pattern of distribution of cyclin A but let us also to presume that observed decrease of expression and its distribution might function as additional factor which could be taken under consideration to establish terminal diagnosis. We are aware of the fact that these are very first observations and that this subject needs to be further investigated with the use of additional methods and samples. Key words: celiac disease, cyclin A, immunogold, immunohistochemistry. PMID: 18999879 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955615 Thu, 13 Nov 2008 15:13:24 +0100 1955615 http://www.medworm.com/index.php?rid=1955614&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999880%26dopt%3DAbstract Authors: Kaczka K, Jakubiak-Wielganowicz M, Zawlik I, Ziolkowski R, Kuzdak K, Pomorski L Immunohistochemistry (IE) and polymerase chain reaction (PCR) are tools enabling to find small number of tumor cells in lymph nodes (LNs) or peripheral blood. Perhaps these methods will allow early detection of cell dissemination and refine risk group within papillary thyroid cancer (PTC) that might benefit from more extensive surgical procedures or adjuvant therapy. In our study we detected PTC cells in the cervical LNs by routine histopathological examination RHE), IE and RT-PCR and compared obtained results. We also estimated the impact of RT-PCR and IE results on TNM staging and clinical staging according to UICC in patients with PTC. Each of 216 LNs from 28 patients with PTC were divided into ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955614 Thu, 13 Nov 2008 15:13:24 +0100 1955614 http://www.medworm.com/index.php?rid=1955613&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999881%26dopt%3DAbstract Authors: Rezacova M, Zaskodova D, Vavrova J, Vokurkova D, Tichy A Valproic acid (VA) possesses anticonvulsant as well as anticancer properties of histondeacetylases inhibitor. Incubation of human promyelocytic leukemia cells HL-60 with VA leads to acetylation of nuclear histones H3 and H4. Using 2 mmol/l concentration we proved the expression of protein p21, which relates to the arrest of cell proliferation and decrease in number of cells in S phase of cell cycle. Treatment of HL-60 cells with VA causes their differentiation, proved as increase in CD11b expression. The most widely used method in cancer treatment is radiotherapy. 24 hours after irradiation by the therapeutical dose of 2 Gy, 56% of HL-60 cells are accumulated in G2 phase of cell cycle. VA had no influence on this accumul... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955613 Thu, 13 Nov 2008 15:13:24 +0100 1955613 http://www.medworm.com/index.php?rid=1955612&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999882%26dopt%3DAbstract In conclusion, the double phase M-SMM and TF-SMM showed favorable diagnostic accuracy in differentiating benign from malignant breast lesions, visually and quantitatively. The optimal visual interpretation grades for the detection of primary breast cancer of double phase M-SMM and TF-SMM were grade and 5. The optimal quantitative indices of M-SMM for the detection of breast cancer were 2.06 for early L/N and 1.72 for delayed L/N. Those of TF-SMM were 3.13 for early, and 2.56 for delayed image. Key words: breast cancer; Tc-99m MIBI; Tc-99m Tetrofosmin. PMID: 18999882 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955612 Thu, 13 Nov 2008 15:13:24 +0100 1955612 http://www.medworm.com/index.php?rid=1955611&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999883%26dopt%3DAbstract Authors: Horacek JM, Tichy M, Pudil R, Jebavy L, Zak P, Ulrychova M, Slovacek L, Maly J Cardiac toxicity of preparative regimen (PR) containing high-dose Cyclophosphamide (120 mg/kg) followed by hematopoietic cell transplantation (HCT) was evaluated with 6 biomarkers of cardiac injury: N-terminal pro brain natriuretic peptide (NT-proBNP), creatine kinase MB (CK-MB mass), cardiac troponins (cTnT, cTnI), heart-type fatty acid binding protein (H-FABP), glycogen phosphorylase BB (GPBB). Twenty-three patients (mean age 44.5+/-10.6 years, 15 males) with acute leukemia were studied. All biomarkers were measured the day before PR, the day after PR, the day after HCT and 14 days after HCT. We found NT-proBNP elevations above 500 ng/L in 6 (26.1 %) patients after PR, in 9 (39.1 %) after HCT and ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955611 Thu, 13 Nov 2008 15:13:24 +0100 1955611 http://www.medworm.com/index.php?rid=1955610&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999884%26dopt%3DAbstract Authors: Kubatka P, Sadlonova V, Kajo K, Machalekova K, Ostatnikova D, Nosalova G, Fetisovova Z Aromatase inhibitor exemestane as a single - agent has no established role in the treatment of premenopausal breast cancer women. The aim of this study was to evaluate preventive effects of exemestane in the model of premenopausal Nmethyl- N-nitrosourea - induced mammary carcinogenesis in female rats. Exemestane treatment begun 7 days prior to carcinogen administration and continued next 12 weeks - till the end of experiment. Exemestane was dietary administered in two concentrations of 1 mg / 1kg (EXE 1), or 10 mg/1 kg (EXE 10), respectively. Exemestane increased the tumor frequency by 80.5 % (P=0.034) in the group EXE 1 and by 61.5 % (P=0.045) in the group EXE 10 in comparison with control ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955610 Thu, 13 Nov 2008 15:13:24 +0100 1955610 http://www.medworm.com/index.php?rid=1955609&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999885%26dopt%3DAbstract This study aimed to compare hormon receptor negative (ER-/PR-/ERBB2+) and triple negative (ER-/PR-/ERBB2-) patients in terms of prognosis and to show that molecularly defined subtypes can be distinguished by conventional laboratory methods. Patients treated between 2001-2007 for hormon receptor negative breast cancer were retrospectively studied. In addition to the conventional prognostic factors, effect of ERBB2 status of the patients on disease-free and overall survival was evaluated. Hormon receptor and ERBB2 status were determined by immunuhistochemistry and fluorescence in-situ hybridization. 141 patients were eligible for the study. Number of patients with ERBB2 positive and triple negative tumors was 70 and 71, respectively, and two groups were comparable in terms of study parameter... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955609 Thu, 13 Nov 2008 15:13:24 +0100 1955609 http://www.medworm.com/index.php?rid=1955608&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18999886%26dopt%3DAbstract In conclusion, we found statistically significant difference of hTERT mRNA presence in plasma of early breast cancer patients when compared to healthy individuals. Second, we found that hTERT mRNA in plasma of early breast cancer patients is affected by the surgical removal of the tumor. Key words: Breast cancer, breast surgery, telomerase, hTERT mRNA. PMID: 18999886 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=1955608 Thu, 13 Nov 2008 15:13:24 +0100 1955608 http://www.medworm.com/index.php?rid=1826366&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665749%26dopt%3DAbstract Authors: Slamenova D, Horvathova E, Marsalkova L, Wsolova L Carvacrol represents a very frequent constituent of essential oils and occurs in many kinds of plants. Though human beings comequite often into close contact with this phenol derivative, its biological effects are not sufficiently known. In this paper we investigated the influence of carvacrol given to rats in drinking water on resistance of their liver and testicular DNA against the oxidative agent hydrogen peroxide H(2)O(2). Carvacrol was dissolved in tap water and given to rats either in concentrations of 30 and 60 mg/1 kg/day during 7 days or in concentrations of 15 and 30 mg/1 kg/day during 14 days. Control animals were given tap water only. After the given time the rats were sacrificed and hepatocytes and testicular cell... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1826366 Thu, 25 Sep 2008 16:16:11 +0100 1826366 http://www.medworm.com/index.php?rid=1826365&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665755%26dopt%3DAbstract Authors: Dolezelova H, Slampa P, Ondrova B, Gombosova J, Sovadinova S, Novotny T, Bolcak K, Ruzickova J, Hynkova L, Forbelska M Positron emission tomography (PET) is used to distinguish between benign and malign tumors, to diagnose relapse or post-therapeutic changes. Lately, PET is used to predict the treatment response. and also a complementary method to determine target volumes in radiotherapy. Daily using of PET in the oncology praxis can change treatment strategy and improve its outcome. Results of this pilot study show the role of PET with 8-F-fluorodeoxyglucose ((18)FDG) for staging of cervical carcinoma and in the radiotherapeutic planning. Between March 2005 and May 2007, 51 patients with cervical carcinoma were treated with combination of external beam radiotherapy and HDR br... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1826365 Thu, 25 Sep 2008 16:16:11 +0100 1826365 http://www.medworm.com/index.php?rid=1671931&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665745%26dopt%3DAbstract Authors: C A This review presents compelling evidence that human glioblastoma is a heterogenous tumor composed from tumor cells and small portion of cancer stem cells - tumor-initiating cells, which have a high tumorigenic potential and a low proliferation rate. Glioma cancer stem cells are phenotypically similar to the normal stem cells, they express CD133 gene and other genes characteristic of neural stem cells and posses the self-renewal potential. Cancer stem cells derived from glioblastoma are capable recapitulate original polyclonal tumors when xenografted to nude mice. They are chemoresistant and radioresistant and therefore responsible for tumor progression and recurrence after conventional glioblastoma therapy. Cancer stem cells contribute to glioma radioresistance by an incre... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671931 Fri, 01 Aug 2008 12:37:46 +0100 1671931 http://www.medworm.com/index.php?rid=1671930&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665746%26dopt%3DAbstract Authors: P V, B M Benefit of adjuvant trastuzumab in breast cancer has been reported in four randomized trials of phase III, and these results are consistent in showing improvement in disease-free survival (DFS). Current evidence for homogeneity of this DFS benefit in subgroups of patients with the different size of the primary HER2-positive tumor treated according to the HERA trial is reviewed. It is evident that current published evidence is insufficient to rule out that there is a cohort of patients with HER2-positive disease who do not achieve a reduction in the risk of recurrence by adjuvant treatment with trastuzumab after completion of previous adjuvant chemo- and radiotherapy. An alternative interpretation of results of the HERA trial currently available in two primary reports ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671930 Fri, 01 Aug 2008 12:37:46 +0100 1671930 http://www.medworm.com/index.php?rid=1671929&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665747%26dopt%3DAbstract Authors: J K Fluorescence intensity (FI) is the basis for classifying phenotypes by fluorescence-label flow cytometry. FI is of a relative value, but with calibration it can be expressed in stoichiometric units called molecules of equivalent soluble fluorochrome (MESF) that reflect the concentrations of the fluorescent conjugates and the receptors they stain. Flow cytometry allows in addition to the determination of positive cells, to establish even the intensity of fluorescent staining, that can be converted into antigen density. The concept of antigen density appears to improve the efficiency of immune techniques in the monitoring of hematopoietic malignancies. Quantitative immunophenotyping is thus suitable for the diagnosis of malignancy, contributes to prognosis and could provide ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671929 Fri, 01 Aug 2008 12:37:46 +0100 1671929 http://www.medworm.com/index.php?rid=1671928&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665748%26dopt%3DAbstract Authors: G K, M P, M B, E M, P R, D S, T KA Polysaccharides represent the major part of the yeast cell wall dry weight and build the skeletal carcass defining cell wall stability and cell morphology (beta-D-glucans) or constitute amorphous matrix and cell surface fibrous material (mannans and mannoproteins). It is known that yeast cell wall beta-D-glucans reveal immunomodulating properties, which allows for their application in anti-infective and antitumor therapy. Recent data also suggest that polysaccharides reveal antioxidant activity that can result in their protective function as antioxidants, antimutagens, and antigenotoxic agents. The paper provides a review of our continuing research involving water-soluble derivatives of beta-D-glucan isolated from the baker's yeast Saccharomy... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671928 Fri, 01 Aug 2008 12:37:46 +0100 1671928 http://www.medworm.com/index.php?rid=1671927&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665749%26dopt%3DAbstract Authors: D S, E H, L M, L W Carvacrol represents a very frequent constituent of essential oils and occurs in many kinds of plants. Though human beings comequite often into close contact with this phenol derivative, its biological effects are not sufficiently known. In this paper we investigated the influence of carvacrol given to rats in drinking water on resistance of their liver and testicular DNA against the oxidative agent hydrogen peroxide (H2O2). Carvacrol was dissolved in tap water and given to rats either in concentrations of 30 and 60 mg/1kg/day during 7 days or in concentrations of 15 and 30 mg/1kg/day during 14 days. Control animals were given tap water only. After the given time the rats were sacrificed and hepatocytes and testicular cells were isolated and treated with dif... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671927 Fri, 01 Aug 2008 12:37:46 +0100 1671927 http://www.medworm.com/index.php?rid=1671926&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665750%26dopt%3DAbstract Authors: A B, L P, L B, L H, J T, P B, E C, J S, J K, M T, J B, Z Z, K M Interphase fluorescence in situ hybridization was used to detect common deletions in B-CLL patients as well as trisomy 12 and aberrations of IgH gene complex at 14q32.33 where we evaluated not only translocation-like signal pattern but also deletions. 120 (82%) patients showed genetic changes - del(13)(q14) 95 (62%), deletion of ATM gene 22 (15%), deletion of p53 gene 25 (17%) and trisomy 12 was proved in 18 (12%) cases. IgH rearrangements were detected in 45 (31%), split of the signals in 11 (8%), deletion of 3 segment flanking IgH gene in 5 (3%) and deletions of variable segment in 29 (20%) patients. Although deletions of 3 segment flanking IgH gene complex are supposed to have an adverse prognostic impact and t... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671926 Fri, 01 Aug 2008 12:37:46 +0100 1671926 http://www.medworm.com/index.php?rid=1671925&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665751%26dopt%3DAbstract Authors: D G, A S, A G, L G, A HB, M SA The aim of this study was to elucidate the effects of hyperthermic treatment on cell morphology and the cytoskeleton in CHO AA8 cell line. The effects of exposure to elevated temperature were analyzed in CHO AA8 cell line by fluorescence microscopy and flow cytometry. The 30min, at 44.5 degrees C heat shock treatment resulted in the collapse of microtubules (MTs) and microfilaments (MFs) around the nucleus followed by their recovery 24h after heating. The initial collapse of these cytoskeletal systems, observed 15min after treatment, was accompanied by the appearance of cells with reduction of volume, shrunken cytoplasm and condensed chromatin. 24h afterwards, there was the increase in the number of cells with restored and extended MT and MF cyto... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671925 Fri, 01 Aug 2008 12:37:46 +0100 1671925 http://www.medworm.com/index.php?rid=1671924&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665752%26dopt%3DAbstract Authors: C GK, M B, B Z Adjuvant treatments reduce the risk for recurrence and death from breast cancer; but even 10-15 years after diagnosis, these risks persist. The aim of our study was to identify prognostic factors for relapse and death in the second decade after primary surgery. Patients with early breast cancer treated from 1983-1987 (n=1035) were included. Patients' characteristics, tumor prognostic factors, treatments, data on recurrence and death were obtained from patients' charts and our cancer registry. Median follow-up was 17 (1-23) years. At 10 years after surgery, 515 (49.8%) patients were alive and of them 432 (41.7%) were relapse-free. Of the 432 patients being alive and relapse-free at 10 years 153 (35.4%) had an event thereafter, of them 38 (25%, 9% of all) had a re... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671924 Fri, 01 Aug 2008 12:37:46 +0100 1671924 http://www.medworm.com/index.php?rid=1671923&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665753%26dopt%3DAbstract Authors: M R, S R, M C, V CM, D R, K K Follicular lymphoma (FL) is characterized by the presence of a t(14;18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671923 Fri, 01 Aug 2008 12:37:46 +0100 1671923 http://www.medworm.com/index.php?rid=1671922&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665754%26dopt%3DAbstract Authors: V B, M L, A V, J L With the aim to evaluate the long term outcome after high-dose chemotherapy and autologous stem cell transplantation (HDCT+ASCT) in patients with relapsed or refractory Hodgkin's lymphoma (HL) we performed a retrospective analysis of patients transplanted at our centre. Between January 1993 and December 2005, 126 consecutive patients with relapsed or refractory HL in the age of 16 to 65 years underwent HDCT+ASCT at our centre and were enrolled in this retrospective analysis. Patients were autografted with either CD34+ positively selected or unmanipulated periferal blood stem cells (PBSC). With a median follow up of 69 months (3-162 months), the actuarial 5-y PFS and OS for all patients after HDCT+ASCT were 59% and 72%, respectively. In patients transplanted ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671922 Fri, 01 Aug 2008 12:37:46 +0100 1671922 http://www.medworm.com/index.php?rid=1671921&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665755%26dopt%3DAbstract Authors: H D, P S, B O, J G, S S, T N, K B, J R, L H, M F Positron emission tomography (PET) is used to distinguish between benign and malign tumors, to diagnose relapse or post-therapeutic changes. Lately, PET is used to predict the treatment response. and also a complementary method to determine target volumes in radiotherapy. Daily using of PET in the oncology praxis can change treatment strategy and improve its outcome. Results of this pilot study show the role of PET with 8-F-fluorodeoxyglucose (18FDG) for staging of cervical carcinoma and in the radiotherapeutic planning. Between March 2005 and May 2007, 51 patients with cervical carcinoma were treated with combination of external beam radiotherapy and HDR brachytherapy, with or without concomitant cisplatin. The lymphatic nodes ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671921 Fri, 01 Aug 2008 12:37:46 +0100 1671921 http://www.medworm.com/index.php?rid=1671920&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665756%26dopt%3DAbstract Authors: D H, Z V, J H, P C, B R, J K, M H Resistin is a member of adipokine family involved in the regulation of inflammatory reactions and insulin sensitivity. In presented study its possible role in the development of benign prostate hyperplasia and prostate cancer was evaluated. Blood samples and prostate specimens were collected from 26 patients with benign prostate hyperplasia (BPH) and from 42 patients with prostate cancer (PCa) stage pT2 (n=18) and pT3 (n=24). Selected metabolic and biochemical parameters and serum resistin levels were measured and anthropometric measurements were performed as well as tissue immunohistochemistry for resistin. Serum resistin levels did not differ significantly between benign hyperplasia and prostate cancer but in cancer patients there was a tren... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671920 Fri, 01 Aug 2008 12:37:46 +0100 1671920 http://www.medworm.com/index.php?rid=1671919&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665757%26dopt%3DAbstract Authors: M HB, R B, P K, J P Nm23-H1/NDPKA and Nm23-H2/NDPKB belong to a large family of NDP kinases, group of structurally and functionally closely related enzymes. The Nm23/NDPs are known to catalyse the transfer of terminal phosphates from ATP to other NTPs and dNTPs. Besides their role in the maintenance of the cells NTP pool the nm23 genes/proteins are known to have additional different biological functions, the most important being its metastasis suppressor activity. The complete picture of roles, actions and targets of nm23 genes/proteins is yet to be discovered. Our goal was to identify the downstream targets of Nm23-H2 by subjecting Nm23-H2 overexpressing CAL 27 cells (oral squamous cell carcinoma of the tongue) to microarray analysis. Using this powerful technology we identif... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671919 Fri, 01 Aug 2008 12:37:46 +0100 1671919 http://www.medworm.com/index.php?rid=1671918&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18665758%26dopt%3DAbstract In conclusion, our results suggest that LMVD predicts tumor stage and lymph node metastasis, and podoplanin-positive tumor cells select a subgroup of tumors with high potential of invasion and metastasis. Key words: gastric cancer, podoplanin, lymphatic microvessel density (LMVD), lymphangiogenesis, prognosis. PMID: 18665758 [PubMed - in process] (Source: Neoplasma) Neoplasma journals http://www.medworm.com/rss/comments.php?id=1671918 Fri, 01 Aug 2008 12:37:46 +0100 1671918 http://www.medworm.com/index.php?rid=1657461&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652043%26dopt%3DAbstract Authors: Vranovsky A, Ladicka M, Lakota J A single center, retrospective analysis evaluating the outcome of patients with poor-risk aggressive non-Hodgkin's lymphoma (NHL) treated with high-dose chemotherapy and autologous stem cell transplantation (ASCT) as a part of firstline therapy. Forty-seven patients younger than 65 years with diffuse large B-cell lymphoma (DLBCL), mantle cell lymphoma (MCL), peripheral T-cell lymphoma (PTCL) or alk-negative anaplastic large cell lymphoma (ALCL) underwent ASCT between July 1997 and November 2005. Patients with DLBCL and alk-negative ALCL had 2 or 3 age-adjusted International Prognostic Index risk factors. All patients were transplanted after MACOP-B induction therapy followed by 2 courses of DHAP and myeloablative chemotherapy BEM or CBV. The co... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1657461 Sun, 27 Jul 2008 10:55:44 +0100 1657461 http://www.medworm.com/index.php?rid=1655798&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18649438%26dopt%3DAbstract Authors: Plesko I, Boyle GS, Ondrusova M, Tomasek L, Kubik A The development of colorectal cancer in former Czechoslovakia and its successor states is illustrated using recorded mortality and from 1968 incidence rates retrieved from National Cancer Registry of Slovakia. The relatively high mortality rates in Czechoslovakia around 1950 contrasted with rates seen in other countries of central, southern and particularly of eastern Europe and were more close to those recorded in affluent countries of western Europe and northern America. Despite continuous stabilisation and decrease of this cancer in high risk countries from late 1970s the unexpected and gradual rise of incidence rates of colorectal cancer was recorded in Slovakia. During the period studied incidence rates rose by an annual... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1655798 Sat, 26 Jul 2008 11:07:29 +0100 1655798 http://www.medworm.com/index.php?rid=1475496&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505336%26dopt%3DAbstract Authors: Brychtova S, Bezdekova M, Brychta T, Tichy M Vascular endothelial growth factors (VEGFs) have a leading role among variety of angiogenic factors. Together with their receptors, they play an important role in endothelial cell proliferation and/or elongation, migration and vascular morphogenesis. In order to determine their possible role in malignant melanoma progression, VEGF (representing VEGFA), VEGF-C and VEGFR-1, -2, -3 immunohistochemical expression on formalin-fixed, paraffin-embedded tissue sections were evaluated. A total of 196 tissue samples consisting of 130 malignant melanomas (MM) with various vertical depth of invasion, 15 metastatic melanomas, and 66 nevi including dysplastic nevi and melanocytic nevi were analysed. Production of both VEGFs were common in benign ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475496 Thu, 29 May 2008 16:20:33 +0100 1475496 http://www.medworm.com/index.php?rid=1475495&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505337%26dopt%3DAbstract The objective of this study was to assess the DNA-protective and anticlastogenic effects of the derivative of a natural compound, carboxymethyl chitin-glucan (CM-CG), against genotoxicity of N-nitrosomorpholine (NMOR) in human hepatoma cells HepG2 and hamster lung cells V79 cultured in vitro. The exponentially growing cells were pre-treated during 24 h with three different concentrations of CM-CG (150, 750 and 1500 mg/ml) and then treated with different concentrations of NMOR. DNAprotective effects of CM-CG were evaluated by single-cell gel electrophoresis (SCGE, comet assay) and anticlastogenic effects by chromosomal aberration assay. At the SCGE assay a short-term (30 min) and at the chromosomal aberration assay a continuous treatment with NMOR was used. In both HepG2 and V79 cells pre-t... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475495 Thu, 29 May 2008 16:20:33 +0100 1475495 http://www.medworm.com/index.php?rid=1475494&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505338%26dopt%3DAbstract Authors: Knopfova L, Smarda J The c-myb protooncogene as well as its transforming derivate, the v-myb oncogene code for transcription factors. They regulate transcription of specific target genes thus controling proliferation, differentiation and apoptosis of hematopoietic cells. Up-regulation of the c-myb expression or rearrangement/amplification of the myb locus are often involved in leukemogenesis. Enforced myb expression blocks differentiation of various leukemic cell lines. Human promonocytes U937 can be induced to differentiate to monocyte/macrophage-like cells using phorbol esters or to granulocytes using retinoic acid. In order to investigate transforming capability of v-myb, we expressed the v-myb oncogene of avian myeloblastosis virus in U937 cells. We found that v-Myb effici... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475494 Thu, 29 May 2008 16:20:33 +0100 1475494 http://www.medworm.com/index.php?rid=1475493&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505339%26dopt%3DAbstract Authors: Pribylova O, Springer D, Svobodnik A, Kyr M, Zima T, Petruzelka L Targeting and tailoring of therapy is the latest trend in breast cancer treatment. The efficacy of the available treatment must be estimated and the probable benefit for the patient determined. The aim of this project was to find out wether also in postmenopausal women chemotherapy can affect hormonal levels in serum and if even the levels of IGF-1 and IGFBP-3 can be changed. In the group of 72 postmenopausal breast cancer patients blood samples were taken before, during and after adjuvant chemotherapy and levels of estradiol, progesterone, LH, FSH, IGF-1 and IGFBP-3 were evaluated. We did not find any statistically significant dependence on tumor stage, expression of hormonal receptors or HER-2 and treatment re... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475493 Thu, 29 May 2008 16:20:33 +0100 1475493 http://www.medworm.com/index.php?rid=1475492&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505340%26dopt%3DAbstract Authors: Cetin E, Cengiz B, Gunduz E, Gunduz M, Nagatsuka H, Bekir-Beder L, Fukushima K, Pehlivan D, N MO, Nishizaki K, Shimizu K, Nagai N Head and neck squamous cell carcinoma (HNSCC) is a diverse group of cancers that are frequently aggressive in their biologic behavior. Inactivation of tumor suppressor gene (TSG) is one of the most critical steps leading to HNSCC. Loss of heterozygosity analysis is very sensitive method for the detection of frequent allelic loss in a chromosomal locus. This method has been considered as an important evidence for the localization of TSGs. We analyzed loss of heterozygosity (LOH) at chromosome 4q22-35 region by using 14 polymorphic microsatellite markers in 83 matched normal and HNSCC tissues. LOH was detected at least in one location in 71 of 83 (86%... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475492 Thu, 29 May 2008 16:20:33 +0100 1475492 http://www.medworm.com/index.php?rid=1475491&cid=s_36595_6_f&fid=36595&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18505341%26dopt%3DAbstract Authors: Porowska H, Paszkiewicz-Gadek A, Lemancewicz D, Bielawski T, Wo Czynski S Transmembrane glycoproteins play a significant role in cancer cells adhesion and metastatic process, just for that reason the glycosylation inhibitors are used to change the glycan structure and in this way the membrane expression of glycoproteins. The inhibitory effect of brefeldin A (BFA) on the expression of some glycoproteins: MUC1 mucin and alpha2beta1 integrin on cell surface of breast (MCF-7 and MDA-MB-231 lines) and endometrial (Ishikawa line) cancer cells was evaluated in our study. In MCF-7 and MDA-MB-231 cells, a decrease in MUC1 expression depended on brefeldin A concentration and equaled about 40% in cells treated with 1mg% of drug. In Ishikawa cells, a decrease in MUC1 expression was lower ... Neoplasma journals http://www.medworm.com/rss/comments.php?id=1475491 Thu, 29 May 2008 16:20:33 +0100 1475491