MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Neuromics' source. Tue, 07 Feb 2012 07:05:47 +0100 http://www.medworm.com/index.php?rid=5665099&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F02%2Fneurotrophins-growth-factors-update.html Our Neurotrophins and Growth Factor Antibodies and Recombinant Proteins help support a wide span of research areas. These areas include: neuroscience, immunology, cardiac disease research and cancer.I would like to update you on recent publications highlighting use of some of these reagents: Aiko Sada, Kazuteru Hasegawa, Pui Han Pin, Yumiko Saga. NANOS2 Acts Downstream of Glial Cell Line-Derived Neurotrophic Factor Signaling to Suppress Differentiation of Spermatogonial Stem Cells. DOI: 10.1002/stem.790. Copyright © 2011 AlphaMed Press...anti-GFRA1 (1:200, Neuromics, Edina, MN)...Images: Ngn3-Cre targets GFRA1-negative cells. (A-F): At P7, Rosa-YFP; Ngn3-Cre double transgenic testes were immunostained with the indicated markers. Most of YFP-positive spermatogonia (Ngn3-lineage c... Neuromics news http://www.medworm.com/rss/comments.php?id=5665099 Sat, 04 Feb 2012 18:06:00 +0100 5665099 http://www.medworm.com/index.php?rid=5657874&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F02%2Faging-caloric-restriction-and-bdnf.html In this study, the investigators found that by restricting calories in rats, DVC BDNF immunoreactive concentrations were elevated and resulting in satiety threshold stability.  This indicates functional desensitization of the DVC to these signals: Karine Bédard, Stéphanie Segura, Stéphanie Mahaut, Catherine Tardivel, Guylaine Ferland, Bruno Lebrun, Pierrette Gaudreau. Effects of aging and caloric restriction on brainstem satiety center signals in rats. Mechanisms of Ageing and Development. dx.doi.org/10.1016/j.mad.2012.01.004.The authors used our BDNF Antibody to determine expression in the DVC.....goat serum and 0.3% Triton-X-100) (Sigma–Aldrich), overnight at 4 °C with chicken anti-rat BDNF antibody (Neuromics, Edina, MN; diluted 1/200 in blocking buffer), rinsed 3×... Neuromics news http://www.medworm.com/rss/comments.php?id=5657874 Fri, 03 Feb 2012 23:35:00 +0100 5657874 http://www.medworm.com/index.php?rid=5636691&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F01%2Flineage-selection-neural-stem-cells-for.html In this study we investigate the effects of controlled Ngn1 expression on mouse ES (mES) cell differentiation in vitro and following grafting into the rat spinal cord. In vitro, Ngn1 expression in mES cells leads to rapid and specific neural differentiation, and a concurrent decrease in proliferation. Similarly transplantation of Ngn1-expressing mES cells into the spinal cord lead to in situ differentiation and spinal precursor formation. These data demonstrate that Ngn1 expression in mES cells is sufficient promote neural differentiation and inhibit proliferation, thus establishing an approach to safely graft ES cells into the spinal cord. Image: Neural progenitors were labeled with anti-rat Nestin polyclonal antibody (Cat#:GT15114) and stained with conjugated donkey anti-goat secondary a... Neuromics news http://www.medworm.com/rss/comments.php?id=5636691 Fri, 27 Jan 2012 00:21:00 +0100 5636691 http://www.medworm.com/index.php?rid=5626096&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F01%2Fearly-diagnosis-of-diabetic-retinopathy.html The earlier the diagnosis the better the outcome. This is especially true with autoimmune diseases like Diabetic Retinopathy (DR). DR is the leading cause of blindness among persons of working age in the industrialized world. Here I feature a publication that shows axoglial alterations at the distal portion of the optic nerve could be the first structural change in the diabetic visual pathway. This could prove good news for discovering better therapies thus preventing blindness: Diego C. Fernandez, Laura A. Pasquini, Damián Dorfman, Hernán J. Aldana Marcos, Ruth E. Rosenstein. Early Distal Axonopathy of the Visual Pathway in Experimental Diabetes. doi:10.1016/j.ajpath.2011.09.018Oligodendrocytes are responsible for insulating axons. Disruptions in the formation of oligodendrocytes could ... Neuromics news http://www.medworm.com/rss/comments.php?id=5626096 Mon, 23 Jan 2012 00:32:00 +0100 5626096 http://www.medworm.com/index.php?rid=5605625&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F01%2Fprimary-neuron-assays-for-studying.html Our goal is to provide our customers and collaborators the tools they need to insure success. This is defined by having the specific Primary Neurons, Growth Factor plus the Markers to meet unique research needs.The proof is in the results. Here are some highlights.Images/Data: FIGURE 5. Microglial p38α MAPK-dependent TNFα is involved in LPS-induced neurite degeneration. (A) Photomicrographs of MAP-2 immunocytochemistry show the morphology of neurons after 72h of co-culture with microglia. The arrow points to the appearance of neurites that have been damaged by LPS-activated WT microglia. In contrast, the arrowhead points to the morphological appearance of healthy, undamaged neurites. (B) Diagram of the Sholl method for quantifying the total number of healthy neurites that inter... Neuromics news http://www.medworm.com/rss/comments.php?id=5605625 Mon, 16 Jan 2012 18:31:00 +0100 5605625 http://www.medworm.com/index.php?rid=5594941&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F01%2Ftrpv1s-in-action.html Our TRPV1s continue to be widely used and published. This recent publication features use out TRPV1-C guinea pig polyclonal for immunohistochemistry and TRPV1-mouse specific for Western Blotting: Sarah E. Canetta, Edlira Luca, Elyse Pertot, Lorna W. Role, David A. Talmage. Type III Nrg1 Back Signaling Enhances Functional TRPV1 along Sensory Axons Contributing to Basal and Inflammatory Thermal Pain Sensation. PLoS ONE 6(9): e25108. doi:10.1371/journal.pone.0025108...IHC: TRPV1 (guinea pig, 1:1000, GP14100 Neuromics); WB: TRPV1 (rabbit, 1:1000, RA14113 Neuromics).Figure. Sensory axons, but not soma, from Type III Nrg1+/− mice show reduced capsaicin responsiveness compared to axons from WT mice. (A) Representative traces of intracellular calcium along sensory axons in response to 1 µM caps... Neuromics news http://www.medworm.com/rss/comments.php?id=5594941 Fri, 13 Jan 2012 15:21:00 +0100 5594941 http://www.medworm.com/index.php?rid=5579084&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2012%2F01%2Fprimary-neurons-vs-pc12-cells-for.html This publication compares PC12 Cells vs E18 Primary Cortical Neurons. The cells showed permeability to some key compounds where the Neurons did not. This demonstrates the importance of including primary neurons in compound testing assays for Neuro-disease research: Wei Zhang , Radhia Benmohamed, Anthony C. Arvanites, Richard I. Morimoto, Robert J. Ferrante, Donald R. Kirsch, Richard B. Silverman. Cyclohexane 1,3-diones and their inhibition of mutant SOD1-dependent protein aggregation and toxicity in PC12 cells. Bioorganic & Medicinal Chemistry. Elsevier Ltd. All rights reserved.doi:10.1016/j.bmc.2011.11.039.Abstract: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons. Currently, there is only one FDA... Neuromics news http://www.medworm.com/rss/comments.php?id=5579084 Sat, 07 Jan 2012 16:37:00 +0100 5579084 http://www.medworm.com/index.php?rid=5535264&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F12%2Fsalivary-neuropeptides-y2s-and-satiety.html A possible new slant for combating obesityThe researchers in this study acheived a sustained increased PYY3-36 (a neuropeptide) expression via viral vector-mediated gene delivery targeting salivary glands and the good news: this increase resulted in a significant long-term reduction in food intake (FI) and body weight (BW). This is evidence for new functions of the previously characterized gut peptide PYY3-36 suggesting a potential simple and efficient alternative therapeutic approach for the treatment of obesity: Andres Acosta1, Maria D. Hurtado1, Oleg Gorbatyuk, Michael La Sala, David Duncan, George Aslanidi, Martha Campbell-Thompson, Lei Zhang, Herbert Herzog, Antonis Voutetakis, Bruce J. Baum, Sergei Zolotukhin. Salivary PYY: A Putative Bypass to Satiety. PLoS ONE 6(10): e26137. doi:10... Neuromics news http://www.medworm.com/rss/comments.php?id=5535264 Fri, 23 Dec 2011 18:50:00 +0100 5535264 http://www.medworm.com/index.php?rid=5474511&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F12%2Fopioid-addiction-during-pregnancy.html In this study, perinatal exposure to low levels of this drug induced accelerated and increased expression of myelin basic proteins (MBPs), cellular and myelin components that are markers of mature oligodendrocytes. In contrast, supra-therapeutic drug doses delayed MBP brain expression and resulted in a decreased number of myelinated axons. We have now found that this biphasic-dose response to buprenorphine can be attributed to the participation of both the l-opioid receptor (MOR) and the nociceptin/orphanin FQ receptor (NOP receptor) in the oligodendrocytes. This is the first study showing the potential role of the NOP receptor in myelination. High levels of opiate exposure could negatively disrupt the normal interplay between these two systems altering the developmental pattern of brain m... Neuromics news http://www.medworm.com/rss/comments.php?id=5474511 Sat, 03 Dec 2011 15:18:00 +0100 5474511 http://www.medworm.com/index.php?rid=5448363&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F11%2Fif-staining-of-human-primary-neurons.html Primary Neurons are inputs or raw materials for cell based assays. When cells do not work as promised, there are multiple costs including lost time and potentially flawed data. Neuromics strives to provide easy to culture, potent and cost effective cells. Proving these capabilities is an ongoing activity for us. This includes testing these cells using our markers. I wanted to share new immunofluorescence images. Here is a link to the protocol: staining primary neurons.hN2 cells stained with our chicken polyclonal antibody to Vimentin, in red. Islands of Hn2 cells form after 4 days in culture forming beautiful flower like structures. Vimentin is a well established marker of early differentiating neuronal lineage cells. Taken with a 10X objective lens. Blue staining is the nuclear DNA.h... Neuromics news http://www.medworm.com/rss/comments.php?id=5448363 Fri, 25 Nov 2011 14:13:00 +0100 5448363 http://www.medworm.com/index.php?rid=5429252&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F11%2Ffear-changes-hippocampus-neuropns.html This study suggests that rapid accumulation of EphrinB2 in hippocampal CA1 neurons is involved in the behavioural and cellular modifications induced by contextual fear conditioning. A similar mechanism does not appear to occur in lateral amygdala neurons, in spite of the robust behavioural and cellular modifications induced in such structure by cued fear conditioning: Antonio Trabalzaa, Sandra Colazingaria, Carmelo Sgobiob, Arturo Bevilacqua. Contextual learning increases dendrite complexity and EphrinB2 levels in hippocampal mouse neurons. Behavioural Brain Research. doi:10.1016/j.bbr.2011.11.008. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5429252 Sun, 20 Nov 2011 16:46:00 +0100 5429252 http://www.medworm.com/index.php?rid=5398260&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F11%2Fdiabetic-retinopathy-blindness-root.html Diabetic retinopathy is a leading cause of acquired blindness. This publication from our friends at University of Buenos Aires touches on potential root causes: Diego C. Fernandez, Laura A. Pasquini, Damián Dorfman, Hernán J. Aldana Marcos, Ruth E. Rosenstein. Early Distal Axonopathy of the Visual Pathway in Experimental Diabetes. doi:10.1016/j.ajpath.2011.09.018" In animals that had been diabetic for 6 weeks, a large increase in astrocyte reactivity occurred in the distal (but not the intraorbital) portion, which coincided with significant axon loss. Moreover, profound myelin alterations and altered morphologic features of oligodendrocyte lineage were observed at the distal (but not the proximal) optic nerve portion. The present results suggest that axoglial alterations at the dist... Neuromics news http://www.medworm.com/rss/comments.php?id=5398260 Fri, 11 Nov 2011 16:48:00 +0100 5398260 http://www.medworm.com/index.php?rid=5398261&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F11%2Fopioid-induced-itch.html Our widely used and frequently published Opioid Receptors Antibodies are used for a spectrum of pain research. Here is an interesting study on the root causes of opioid induced itch.Xian-Yu Liu, Zhong-Chun Liu, Yan-Gang Sun, Michael Ross1, Seungil Kim, Feng-Fang Tsai, Qi-Fang Li, Joseph Jeffry, Ji-Young Kim, Horace H. Loh, Zhou-Feng Chen. Unidirectional Cross-Activation of GRPR by MOR1D Uncouples Itch and Analgesia Induced by Opioids. Cell, Volume 147, Issue 2, 14 October 2011, Pages 261-262.Root Causes: Spinal opioid-induced itch, a prevalent side effect of pain management, has been proposed to result from pain inhibition. We now report that the μ-opioid receptor (MOR) isoform MOR1D is essential for morphine-induced scratching (MIS), whereas the isoform MOR1 is required only for morphine... Neuromics news http://www.medworm.com/rss/comments.php?id=5398261 Tue, 08 Nov 2011 19:08:00 +0100 5398261 http://www.medworm.com/index.php?rid=5356857&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F10%2Fmouse-episcs-into-myelinating-cells.html This study published recently in Nature Methods hit my radar scope becaused it referenced use of our widely used and frequently published stem cell marker Tuj 1 (Neuron-specific class III beta-tubulin): Fadi J Najm, Anita Zaremba, Andrew V Caprariello, Shreya Nayak, Eric C Freundt, Peter C Scacheri, Robert H Miller & Paul J Tesar. Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells. Nature Methods (2011) doi:10.1038/nmeth.1712. Dr. Paul Tesar and his team at Case Western University demonstrated the ability to convert pluripotent epiblast stem cells into pure populations of myelinating cells, called oligodendrocyte progenitor cells (OPCs). First, stem cells in a petri dish are treated with molecules to direct them to become the most primit... Neuromics news http://www.medworm.com/rss/comments.php?id=5356857 Wed, 26 Oct 2011 17:38:00 +0100 5356857 http://www.medworm.com/index.php?rid=5332767&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F10%2Fimmunostaining-neurons-and-glia.html I would like to thank Dr. Gerry Shaw, University of Florida for his excellent work with our Primary Neurons and Astrocytes and Neuronal-Glial Markers. Here's an example image with many more to follow:Image: E18 hippocampal neurons stained with MAPT (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites, but doublecortin is more abundant in the growth cones and periphery. As a result, the periphery appears green while the more proximal regions of the cells are yellow. The single longer process of this cell, presumably an axon, has a low doublecortin content and so appears red. Blue staining is the nuclear DNA. Protocol on datasheet. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5332767 Thu, 20 Oct 2011 11:25:00 +0100 5332767 http://www.medworm.com/index.php?rid=5302468&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F10%2Fbdnf-and-exercise-study-running-mice.html In this study researchers show new cell proliferation, survival, neuron number, and neurotrophin levels were enhanced only when running was accessible to mice. They conclude that exercise is the critical factor mediating increased BDNF levels and adult hippocampal neurogenesis: Tali Kobilo, Qing-Rong Liu, Kriti Gandhi, Mohammed Mughal, Yavin Shaham and Henriette van Praag. Running is the neurogenic and neurotrophic stimulus in environmental enrichment. doi: 10.1101/lm.2283011. Learn. Mem. 2011. 18: 605-609... human recombinant BDNF (0.1 µg) monomer (Neuromics)...BDNF, CF Recombinant ProteinRelated Reagents:Neuron-Glial Expressed-Includes Neurotrophin ProteinsNeurotrophins and Growth Factor AntibodiesPrimary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocyt... Neuromics news http://www.medworm.com/rss/comments.php?id=5302468 Mon, 10 Oct 2011 01:01:00 +0100 5302468 http://www.medworm.com/index.php?rid=5235421&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F09%2Fimmune-inflammatory-response-and-pain.html Our Pain and Inflammation Related Research Antibodies are increasingly being used to study the root causes of immune/inflammatory related pain induction. Here're related publications: Lintao Qu, Pu Zhang, Robert H. LaMotte, Chao Ma. Neuronal Fc-gamma receptor I mediated excitatory effects of IgG immune complex on rat dorsal root ganglion neurons. Brain, Behavior, and Immunity. Volume 25, Issue 7, October 2011, Pages 1399-1407......rabbit-anti-TRPV1, 1:1000, Neuromics...Highlights: Pain often accompanies antigen-specific immune-related disorders though little is known of the underlying neural mechanisms. A common feature among these disorders is the elevated level of antigen-specific immunoglobulin (Ig) G in the serum and the presence of IgG immune complex (IC) in the affected tissue. We hy... Neuromics news http://www.medworm.com/rss/comments.php?id=5235421 Mon, 19 Sep 2011 16:33:00 +0100 5235421 http://www.medworm.com/index.php?rid=5235422&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F09%2Fxona-microfluidics-and-neurons.html I am impressed with these Video from the Jeon Lab at UC Irvine. It represents a novel method for neuro-drug discovery:Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device.This technology represents a way to separate axon from cell bodies. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5235422 Thu, 15 Sep 2011 20:47:00 +0100 5235422 http://www.medworm.com/index.php?rid=5191950&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F09%2Fhnp1-human-neural-progenitors-video.html Video Protocols designed to insure your success with our hNP1TM Human Neural ProgenitorshNP1™ Human Neural Progenitors Video Protocols Thawing, Culturing and Transfer-Sub Culturing Protocols. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5191950 Sat, 03 Sep 2011 16:50:00 +0100 5191950 http://www.medworm.com/index.php?rid=5181043&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fopioid-receptor-antibodies-trifecta.html This publication proposes a role for opioid receptors in treating cancers. It also references use of our μ, δ, and κ opioid receptor antibodies.Kohei Yamamizu1, Sadayoshi Furuta, Shiori Katayama, Michiko Narita, Naoko Kuzumaki, Satoshi Imai, Hiroshi Nagase, Tsutomu Suzuki, Minoru Narita, and Jun K. Yamashita. The κ opioid system regulates endothelial cell differentiation and pathfinding in vascular development. Blood July 21, 2011 vol. 118 no. 3 775-785.Highlights: The opioid system is, thus, a new regulator of vascular development that simultaneously modifies 2 distinct vascular properties, EC differentiation and vascular pathfinding. We confirmed that KOR, but not MOR, was highly expressed in various ECs such as HUVECs (data not shown), suggesting that KOR agonists could directly act... Neuromics news http://www.medworm.com/rss/comments.php?id=5181043 Tue, 30 Aug 2011 12:44:00 +0100 5181043 http://www.medworm.com/index.php?rid=5169369&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fis-neuropathy-really-gliopathy.html I found this excellent website from posting by Dr. Jan M. Keppel Hesselink, Professor molecular pharmacology, director Institute neuropathic pain: http://www.neuropathie.nu/. It represents a new way of understanding root causes and potential therapies for Neuropathic Pain. Here're highlights: Gliopathic pain: is a brand new term for what we always thought to be neuropathic pain. It refers to pain related to neuropathic pain, however, the primary driver of this pain is most probably more linked to glia and asterocytes. The mechanism of gliopathic pain is the hyperactivation of glia cells, which results in neuropathic pain.The role of Glia and Astrocytes:Glia and astrocytes play a central role in neuropathic pain, and gliopathic pain, or asteropathic pain will become new synonyms for neuropa... Neuromics news http://www.medworm.com/rss/comments.php?id=5169369 Sat, 27 Aug 2011 16:20:00 +0100 5169369 http://www.medworm.com/index.php?rid=5158144&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fprimary-neurons-and-cell-based-assays.html The feedback I receive from Neuroscientists is consistent. To paraphrase, "gives us healthy, consistent and potent primary cells. I understand the hard work it takes to generate meaningful and publishable results from cell based assays. Our Primary Neurons and Astrocytes are merely inputs for these assays. The real cost is the time invested in culturing and time lost if they don't work.I have numerous postings on success: Primary Neurons Postings. I wanted to share more data and feedback. Primary DRGs-Culturing these can be tricky. I make it a point to work with labs to make sure the protocol options best match the desired outcome for assays. This includes replacing cells to make sure we can accurately troubleshoot. This approach insures I can pin point the issues and make sure t... Neuromics news http://www.medworm.com/rss/comments.php?id=5158144 Thu, 25 Aug 2011 19:49:00 +0100 5158144 http://www.medworm.com/index.php?rid=5158145&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fmor-and-nmdar-interplay-implications-in.html This study drills down into the specifics of this regulation and references use of Neuromics' MOR1C Antibody: María Rodríguez-Muñoz, Pilar Sánchez-Blázquez, Ana Vicente-Sánchez, Esther Berrocoso and Javier Garzón. María Rodríguez-Muñoz, Pilar Sánchez-Blázquez, Ana Vicente-Sánchez, Esther Berrocoso and Javier Garzón. The Mu-Opioid Receptor and the NMDA Receptor Associate in PAG Neurons: Implications in Pain Control. Neuropsychopharmacology , (3 August 2011) | doi:10.1038/npp.2011.155.Abstract: The capacity of opioids to alleviate inflammatory pain is negatively regulated by the glutamate-binding N-methyl-D-aspartate receptor (NMDAR). Increased activity of this receptor complicates the clinical use of opioids to treat persistent neuropathic pain. Immunohistochemical and ultrast... Neuromics news http://www.medworm.com/rss/comments.php?id=5158145 Tue, 23 Aug 2011 01:07:00 +0100 5158145 http://www.medworm.com/index.php?rid=5158146&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fplasma-netrin-1-is-diagnostic-biomarker.html Conclusion: Our results suggest that plasma netrin-1 can be used as a diagnostic biomarker for many human cancers. Image: Immunohistochemical localization of netrin-1 in renal cell carcinoma (RCC) tissues. A. Secondary antibody control showing no staining. B. Normal adjacent tissues do not show any staining for netrin-1. C. Stage I RCC shows staining for netrin-1. D. Stage II RCC shows staining for netrin-1. E-F.Netrin-1 Immunohistochemistry: Stage I–III renal cell carcinoma and normal tissue section (Tissue Array) was obtained from Biomax to immunolocalize netrin-1, as described previously (29). Briefly, tissue sections were dewaxed and rehydrated with graded ethanol (100%, 90%, 70% and 30%) and then washed with PBS. Antigen retrieval was carried out using citrate buffer and steamer. Th... Neuromics news http://www.medworm.com/rss/comments.php?id=5158146 Thu, 18 Aug 2011 14:33:00 +0100 5158146 http://www.medworm.com/index.php?rid=5125559&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fsox2-and-initiation-of-breast-tumors.html I consider it a feather in Neuromics' cap when our regent(s) are referenced in a Nature Journal. More importantly it enables me to keep the pulse on novel and important discovery.This pub references use of one of our SOX2 Antibody and comes from Dr. Angel García Martín and his Team at INBIOMED: O Leis, A Eguiara, E Lopez-Arribillaga, M J Alberdi, S Hernandez-Garcia, K Elorriaga, A Pandiella, R Rezola and A G Martin. Sox2 expression in breast tumours and activation in breast cancer stem cells. Oncogene , (8 August 2011) | doi:10.1038/onc.2011.338.The important insight from this study is: "Over-expression of Sox2 increased mammosphere formation, effect dependent on continuous Sox2 expression; furthermore, Sox2 knockdown prevented mammosphere formation and delayed tumour... Neuromics news http://www.medworm.com/rss/comments.php?id=5125559 Sat, 13 Aug 2011 00:39:00 +0100 5125559 http://www.medworm.com/index.php?rid=5107191&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F08%2Fhigh-content-and-high-throughput.html Kits designed for Drug Discovery and DevelopmentOur customers have been impressed with the capablities of our in vivo and in vitro apoptosis and toxicity kits. Here's a recent pub referencing use of one of our FLICA™ in vitro Caspase Kits: Giovanna Grandinetti, Nilesh P. Ingle, and Theresa M. Reineke. Interaction of Poly(ethylenimine)–DNA Polyplexes with Mitochondria: Implications for a Mechanism of Cytotoxicity. Mol. Pharmaceutics, Article ASAP Publication Date (Web): June 23, 2011 Copyright © 2011 American Chemical Society...inhibitor of caspases (FLICA) specific for caspase-9 (Neuromics, Inc)...Images: Jurkat cells were treated with 1 µM staurosporine for 3 hours to induce caspase 9 activity (top), or were treated with a control (bottom). Both populations were incubated with ICT�... Neuromics news http://www.medworm.com/rss/comments.php?id=5107191 Sun, 07 Aug 2011 15:06:00 +0100 5107191 http://www.medworm.com/index.php?rid=5085774&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Ftrpv1-and-diabetic-neuropathy.html Conclusions: From these results, it is concluded that TRPV1 is an integral component of initiating and maintaining inflammatory thermal hyperalgesia, which can be alleviated by intrathecal administration of RTX. Further, the results suggest that enhanced expression and inflammation-induced sensitization of TRPV1 at the spinal cord may play a role in central sensitization in STZ-induced neuropathy.Therapies that downregulate or silence TRPV1 expression could be the key to better treatments for the Thermal Algesia cause by diabetes. I will keep you posted. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5085774 Sat, 30 Jul 2011 15:49:00 +0100 5085774 http://www.medworm.com/index.php?rid=5077368&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Fhnp1-thawing-protocolmp4.html This is a great video that shows thawing + plating protocol for our NP1 Cells! (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5077368 Fri, 29 Jul 2011 14:21:00 +0100 5077368 http://www.medworm.com/index.php?rid=5069164&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Fpotent-and-cost-effective-cell-based.html I have had many conversations with basic and drug discovery researchers on improving cell based assays. Here's the wish list:More potent cells/mediaMore accurate analytic tools-quatititative and reproducible resultsAbility to use cells and tools in high throughput/high content screening.Cost effectivenessThis wish list is front and center in determining the cells/media and related tools we add to Neuromics' offerings. We are pleased to announce the addition of our Hemogenix's Bioluminomics™ In-Vitro Cell Assays, MSCGro™ Mesenchymal Stem Cell Media and Umbilical Cord Blood derived hMesenchymal Stem Cells.These provide quantitation, not subjectivity. It includes assay calibration and standardization. It means assay validation. It produces results you can trust and rely on. It means innov... Neuromics news http://www.medworm.com/rss/comments.php?id=5069164 Wed, 27 Jul 2011 16:13:00 +0100 5069164 http://www.medworm.com/index.php?rid=5057631&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Fdifferential-healing-properties-of.html Conclusions: This study shows for the first time that hACL-SCs are intrinsically different from hMCL-SCs. We suggest that the differences in their properties contribute to the known disparity in healing capabilities between the two ligaments.I will be posting more on autologous stem cell therapies research. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=5057631 Sat, 23 Jul 2011 21:39:00 +0100 5057631 http://www.medworm.com/index.php?rid=5057632&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Funderstanding-rhett-syndrome.html Dr Jeffrey Neul and his team at Baylor Medical College have been studying the root causes of pathologies associated with Rett Syndrome. This disease is a neurodevelopmental disorder caused by mutations in methyl-CpG-binding protein 2 (MECP2), a transcriptional regulator. In addition to cognitive, communication, and motor problems, affected individuals have abnormalities in autonomic function and respiratory control. Sufferers often die young due to these abnormalities.They found that MeCP2 is necessary within the brainstem and spinal cord for normal lifespan, normal control of heart rate, and respiratory response to hypoxia. Here's the exciting news: restoration of MeCP2 in a subset of the cells in this same region is sufficient to rescue abnormal heart rate and abnormal respiratory respon... Neuromics news http://www.medworm.com/rss/comments.php?id=5057632 Thu, 21 Jul 2011 15:17:00 +0100 5057632 http://www.medworm.com/index.php?rid=5049789&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Funderstanding-rhett-syndrome.html Dr Jeffrey Neul and his team at Baylor Medical College have been studying the root causes of pathologies associated with Rhett Syndrome. This disease is a neurodevelopmental disorder caused by mutations in methyl-CpG-binding protein 2 (MECP2), a transcriptional regulator. In addition to cognitive, communication, and motor problems, affected individuals have abnormalities in autonomic function and respiratory control. Sufferers often die young due to these abnormalities.They found that MeCP2 is necessary within the brainstem and spinal cord for normal lifespan, normal control of heart rate, and respiratory response to hypoxia. Here's the exciting news: restoration of MeCP2 in a subset of the cells in this same region is sufficient to rescue abnormal heart rate and abnormal respiratory respo... Neuromics news http://www.medworm.com/rss/comments.php?id=5049789 Thu, 21 Jul 2011 15:17:00 +0100 5049789 http://www.medworm.com/index.php?rid=5007673&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F07%2Fguinea-pig-p2x3-update-good-news.html I have had to say to many customers, "our guinea pig P2x3 is on backorder". The increasing number of pubs referencing this antibody only amped demand.We tried and tried to re-make it. The result was none of the bleeds we tested had a signal strong enough to release the antibody. We had a customer suggest re-testing several of the more promising bleeds. Thank you! We have good news on results and we are offering for 50% off. This is to acknowledge the investment required for TSA and Guinea Pig Biotinylated Antibody.Here're the recent pubs I referenced:Gabriela Castañeda-Corral, Héctor I. Rocha-González, Beatriz Godínez-Chaparro, Juan Miguel Jiménez-Andrade and Vinicio Granados-Soto. Role of the spinal Na+/H+ exchanger in formalin-induced nociception. Neuroscience Letters. ... Neuromics news http://www.medworm.com/rss/comments.php?id=5007673 Thu, 07 Jul 2011 02:07:00 +0100 5007673 http://www.medworm.com/index.php?rid=4952538&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F06%2Ftransfectioninfection-of-primary.html Gene Expression Analysis of Neurons is an important tools in basic research and the study of neuropathologies. At the Neuromics' blog: "siRNA, DsiRNA and Plasmid Transfection Efficiency", I have posted many examples of successful tarnsfection of primary neurons and related cells using both our Transfection Kits/Reagents and others.The other puzzle piece for these studies is having a fresh, pure and easy to use source of cells. Here, Neuromics has many options. These primary neurons and neural progenitors are widely referenced in key publications. Applications referenced include: transfection, pharmacology, electrophysiology, immunocytochemistry, and neuronal development studies.This posting features infection of our e18 Primary Rat Combined Hippocampus, Cortex, and Ventricular Neurons... Neuromics news http://www.medworm.com/rss/comments.php?id=4952538 Mon, 20 Jun 2011 14:04:00 +0100 4952538 http://www.medworm.com/index.php?rid=4944787&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F06%2Fchronic-type-ii-diabetes-mellitus-and.html Our Neuropeptide and Neuropeptide Receptors and Leptin and Leptin Receptor Antibdodies are frequently used to study pathologies and biology specific to Obesity and Diabetes.Here's a new publication studying the relationship between diabetic neuropathy and altered neuropeptide Y and its receptor expression levels in myocardium and plasma.Robina Matyal, Feroze Mahmood, Michael Robich, Hiliary Glazera, Kamal Khabbaza, Philip Hessa, Cesario Bianchia, Robert Hagberga, Shu-Xu Hua, and Frank W. Sellkea. Chronic type II diabetes mellitus leads to changes in neuropeptide Y receptor expression and distribution in human myocardial tissue. European Journal of Pharmacology. Volume 665, Issues 1-3, 31 August 2011, Pages 19-28. Abstract: Neuropeptide Y is one of the most abundant neur... Neuromics news http://www.medworm.com/rss/comments.php?id=4944787 Sat, 18 Jun 2011 00:45:00 +0100 4944787 http://www.medworm.com/index.php?rid=4933512&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F06%2Fsox2-as-marker-for-melanoma.html I would like to post a new application for our SOX2 neural progenitor marker. Alvaro C. Laga, Qian Zhan,Carsten Weishaupt, Jie Ma, Markus H. Frank, George F. Murphy. SOX2 and nestin expression in human melanoma: an immunohistochemical and experimental study. Experimental Dermatology. Volume 20, Issue 4, pages 339–345, April 2011. DOI: 10.1111/j.1600-0625.2011.01247.x.SOX2 is an embryonic neural crest stem-cell transcription factor recently shown to be expressed in human melanoma and to correlate with experimental tumor growth. SOX2 binds to an enhancer region of the gene that encodes for nestin, also a neural progenitor cell biomarker. To define further the potential relationship between SOX2 and nestin, we examined co-expression patterns in 135 melanomas and 37 melanocytic nevi. Immunoh... Neuromics news http://www.medworm.com/rss/comments.php?id=4933512 Thu, 16 Jun 2011 01:18:00 +0100 4933512 http://www.medworm.com/index.php?rid=4933513&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F06%2Fassembly-and-maintenance-of-gabaergic.html Understanding the mechanisms underlying Axon Growth and Guidance is key to finding the root cause of neurological diseases and discovering potential therapies.In this important study, researchers TrkB is required for the localization of an Ig superfamily cell adhesion molecule, Contactin-1, in Golgi and granule cells and the absence of Contactin-1 also results in deficits in inhibitory synaptic development. This demonstrates that TrkB controls the assembly and maintenance of GABAergic synapses and suggest that TrkB functions, in part, through promoting synaptic adhesion: TrkB (Tropomyosin-Related Kinase B) Controls the Assembly and Maintenance of GABAergic Synapses in the Cerebellar Cortex. The Journal of Neuroscience, February 23, 2011 • 31(8):2769 –2780 ... Neuromics news http://www.medworm.com/rss/comments.php?id=4933513 Sun, 12 Jun 2011 14:51:00 +0100 4933513 http://www.medworm.com/index.php?rid=4883194&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F05%2Fleprb-stat3-pathway-and-obesity.html Neuromics' Hypothalumus Neurons, Leptin Antibodies and Recombinant Proteins are being increasingly used by researchers studying root causes of diabetes and obesity. I am pleased to update you on an important study from our friends at Shanghai Jiaotong University School of Medicine. This publication references use of our LepRb/OBRb Antibody. Pei Wang, Feng-Jiao Yang, Hui Du, Yun-Feng Guan, Tian-Ying Xu, Xue-Wen Xu, Ding-Feng Su, and Chao-Yu Miao. Involvement of Leptin Receptor Long Isoform (LepRb)-STAT3 Signaling Pathway in Brain Fat Mass– and Obesity-Associated(FTO) Downregulation during Energy Restriction. © 2011 The Feinstein Institute for Medical Research, www.feinsteininstitute.org.Online address: http://www.molmed.org. doi: 10.2119/molmed.2010.00013.Abstract: Obesity is an imp... Neuromics news http://www.medworm.com/rss/comments.php?id=4883194 Sun, 29 May 2011 15:43:00 +0100 4883194 http://www.medworm.com/index.php?rid=4871695&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F05%2Fbradykinin-b2-or-purinergic-p2y.html In this study the authors probe the role of a particular nerve cell potassium current, called the M-current, in the control of neurotransmitter release, using the contraction rate of the co-cultured heart cells as a functional read-out of noradrenaline release. Using several drugs and receptor agonists, we manipulated the activity of M-current in the nerve cells, which were stimulated by nicotine, and monitored its effect on heart cell beating. We find that the M-type potassium current has a robust role in the control of noradrenaline release from the nerve cells, and in the response of the heart cells to increased beating frequency as a result:Oleg Zaika, Jie Zhang, and Mark S. Shapiro. Functional role of M-type (KCNQ) K+ channels in adrenergic control of cardiomyocyte contraction rate by... Neuromics news http://www.medworm.com/rss/comments.php?id=4871695 Fri, 27 May 2011 14:03:00 +0100 4871695 http://www.medworm.com/index.php?rid=4840944&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F05%2Fmcp-1-promotes-lung-inflammation.html Providing reaserchers Immune/Inflammatory Response Markers is a growing business for us. Publications referencing their use is a key validation that they can do the job. Here's a recent pub that references use of our goat anti-mouse RAGE polyclonal antibody. More importantly, it studies the role of Monocyte chemoattractant protein 1 (MCP-1) in modulating inflammatory response in the lungs: Marieke A.D. van Zoelen, Marleen I. Verstege, Christian Draing, Regina de Beer, Cornelis van’t Veera, Sandrine Florquin, Paul Bresser, Jaring S. van der Zee, Anje A. te Velde, Sonja von Aulock and Tom van der Poll. Endogenous MCP-1 promotes lung inflammation induced by LPS and LTA. doi:10.1016/j.molimm.2011.04.001. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4840944 Tue, 17 May 2011 12:38:00 +0100 4840944 http://www.medworm.com/index.php?rid=4802065&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F05%2Fagrps-role-in-energy-homeostasis.html This blog has featured various posting on the building blocks and involved in Energy Homeostasis. This is key to future therapies for Obesity and Diabetes. Here researchers study: Yongheng Cao1, Masanori Nakata, Shiki Okamoto, Eisuke Takano, Toshihiko Yada, Yasuhiko Minokoshi, Yukio Hirata, Kazunori Nakajima, Kristy Iskandar, Yoshitake Hayashi, Wataru Ogawa, Gregory S. Barsh, Hiroshi Hosoda, Kenji Kangawa, Hiroshi Itoh, Tetsuo Noda, Masato Kasuga, Jun Nakae.PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure. PLoS ONE 6(4): e18324. doi:10.1371/journal.pone.0018324.IHC Protocol: For immunofluorescence analyses, mice were transcardially perfused with saline followed by 4% paraformaldehyde in 0.1 M phosphate-bu... Neuromics news http://www.medworm.com/rss/comments.php?id=4802065 Sun, 08 May 2011 16:38:00 +0100 4802065 http://www.medworm.com/index.php?rid=4663716&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F03%2Fgfap-and-mouse-myenteric-plexus.html Our Neuronal-Glial Markers are important tools for our customers investigating expression in the CNS and PNS. I have posted images showing staining of mouse retinal astrocytes and in the ventral horn, funiculus of adult rat spinal cord and mouse medulloblastoma stem cells using our GFAP antibodies.I wanted to share an excellent image generated by Dr. Kate Ellacott's lab at Vanderbilt University.GFAP (Chicken-Cat#: CH22102) staining in of enteric glia in the myenteric plexus of the mouse gastrointestinal tract. Staining was performed in methanol/acetone fixed frozen sections using 1:1000 dilution of the antibody followed by 1:500 anti-chicken Alexa 594. Related Reagents:GFAP (Chicken-Cat#: CH23011)GFAP (Mouse-Cat#; MO15052)GFAP (Rabbit-Cat#:RA19063)Neurona... Neuromics news http://www.medworm.com/rss/comments.php?id=4663716 Fri, 01 Apr 2011 00:09:00 +0100 4663716 http://www.medworm.com/index.php?rid=4626656&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F03%2Fstemez-hnp1-neural-progenitors-and-ion.html In my conversation with neuro-drug discover researchers, I am frequently being asked about the potential of using our STEMEZ(TM) hNP1 Human Neural Progenitors Expansion Kits for studying ion channels. How effective are these cells as a source for studying neurodegenerative diseases and for drug screening assays? There is good news.When differentiated, these  neural progenitors express subunits of glutamatergic,  GABAergic, nicotinic, purinergic and transient receptor potential receptors. In addition, sodium  and calcium channel subunits were also expressed. Functionally, virtually all the NP cells exhibited delayed rectifier potassium channel currents and some differentiated cells exhibited  tetrodotoxin sensitive, voltage-dependent sodium channel current under who... Neuromics news http://www.medworm.com/rss/comments.php?id=4626656 Thu, 24 Mar 2011 01:25:00 +0100 4626656 http://www.medworm.com/index.php?rid=4591727&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F03%2Fperk12-trpv1-and-scalding-burn-pain.html I can only imaging the intense pain suffered by people with scalding pain injuries.Understanding the bio-processes behind this form of nociceptive pain is a step towards finding better analgesics. In  this study, the authors study several key markers for pain...phosphoERK1/2 and TRPV1 (surprisingly not a major piece of the this pain signaling process).John P.M. Whitea, Chin Wing Koa, Antonio Rei Fidalgoa, Mario Cibellia, Cleoper C. Paulea, Peter J. Andersona, Celia Cruzb, Szabolcs Gombad, Klara Matesze, Gabor Veressd, Antonio Avelinob and Istvan Nagya. Severe burn injury induces a characteristic activation of extracellular signal-regulated kinase 1/2 in spinal dorsal horn neurons. European Journal of Pain.doi:10.1016/j.ejpain.2010.12.006...pERK1/2 (1:1000, Neuromics; RA15002... Neuromics news http://www.medworm.com/rss/comments.php?id=4591727 Tue, 15 Mar 2011 21:06:00 +0100 4591727 http://www.medworm.com/index.php?rid=4565724&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F03%2Ftrkb-and-assemblymaintenance-of.html Conclusion Highlights:  Kinase activity of TrkB is required not simply to initiate GABAergic synapse formation, but also to maintain these synapses in adulthood. We also show that the localization of Contactin-1 at the synaptic contacts between Golgi and granule cells requires TrkB suggesting that TrkB promotes synapse formation and maintenance, in part, by controlling the localization of cell adhesion molecules.Related Reagents:Contactin-1, FC Chimera Recombinant ProteinContactin-4 Recombinant ProteinCaspr2 Antibody  Axon Growth and Guidance Research AntibodiesAxonal Growth and Guidance Research Recombinant ProteinsOligodendrocyte or Oligodendroglial MarkersPrimary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes  (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4565724 Wed, 09 Mar 2011 16:52:00 +0100 4565724 http://www.medworm.com/index.php?rid=4507082&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F02%2Fnotch-signaling-and-neurodegenerative.html Conclusion: In disc cells, hypoxia promotes expression of notch signaling proteins. Notch signaling is important to maintain proliferation of disc cells and hence offers a therapeutic target to restore cell number during degenerative disc disease.Hope for sufferers of degenerative disease disc disease. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4507082 Mon, 21 Feb 2011 20:33:00 +0100 4507082 http://www.medworm.com/index.php?rid=4470174&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F02%2Fh9-wa09-derived-human-neural.html Neuromics offers stable, potent and well characterized STEMEZ (TM) Human Neural Progenitor & Neuron Discovery Kits. These are derived from NIH registered human ES cell line H9 (WA09).Details on the capabilities of these cells are detailed in a variety of publications. They work! As a result, I am trying to make it easier to justify using them when and where they are needed. In addition to having frequent updates on new references, methods and data, I want to offer the best pricing possible.In researching pricing of cells derived from the same parental line and having similar characteristics, I noticed prices ranging from 995 to 2800 USD. Our pricing starts at 695 USD and we offer deeper discounting if required. I do not want price to be a barrier. Here's some sample data on these cells... Neuromics news http://www.medworm.com/rss/comments.php?id=4470174 Sat, 12 Feb 2011 15:56:00 +0100 4470174 http://www.medworm.com/index.php?rid=4445555&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F02%2Fmore-on-dor.html I am always delighted when we recieve positive feedback on one of our new reagents. It is even better when a researcher graciously shares images. I would like to thank Dr. Prof. Dr. Rapheal Sell of Freie Universität Berlin for sharing these excellent Immunofluorescence images:Images: IF detection of delta-opioid receptors in tissue engineered human oral mucosa models using Delta Opioid Receptor 361-372. The tissues were frozen at -80°C, cut into 8 µm slices and fixed with formaldehyde solution. Blocking was performed with goat serum (dilutet 1:20 in PBS) for 30 minutes at RT. Primary antibody dilutions in PBS/BSA/Tween-20 were 1:500, 1:1000 and 1:1500, slides were incubated overnight at 4°C. Secondary (fluorescent) antibody was diluted 1:400, slides were incubated for 30 minutes at roo... Neuromics news http://www.medworm.com/rss/comments.php?id=4445555 Mon, 07 Feb 2011 22:09:00 +0100 4445555 http://www.medworm.com/index.php?rid=4405445&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F01%2Fpink1-and-alzheimers-and-multiple.html I would like to highlight a recent publication referencing use of our PTEN-induced kinase/PINK1 Antibody.Jack Van Horssen et al. investigated PINK1 expression in well-characterized brain samples derived from MS and AD individuals using immunohistochemistry.Micha M.M. Wilhelmus, Susanne M.A. van der Pol, Quentin Jansen, Maarten E. Witte, Paul van der Valk, Annemieke J.M. Rozemuller, Benjamin Drukarch, Helga E. de Vries and Jack Van Horssen. Association of Parkinson disease-related protein PINK1 with Alzheimer disease and multiple sclerosis brain lesions. Free Radical Biology and Medicine. Volume 50, Issue 3, 1 February 2011, Pages 469-476.Abstract: Mitochondrial dysfunction and oxidative stress are hallmarks of various neurological disorders, including multiple sclerosis (MS), Alz... Neuromics news http://www.medworm.com/rss/comments.php?id=4405445 Thu, 27 Jan 2011 01:19:00 +0100 4405445 http://www.medworm.com/index.php?rid=4355603&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F01%2Fupregulations-of-p2x3-and-asic3-involve.html It gets my attention when several of our ion channel markers are referenced in the title of a publication.Kiyomi Hori, Noriyuki Ozaki, Shigeyuki Suzuki, Yasuo Sugiura. Upregulations of P2X3 and ASIC3 involve in hyperalgesia induced by cisplatin administration in rats. PAIN 149 (2010) 393–405Findings: "We explored the role of ion channels expressed in DRG neurons in the painful neuropathy associated with cisplatin administration. Upregulations of TRPV2, P2X3 and ASIC3 may play important roles in the mechanical hyperalgesia induced by cisplatin. In addition to cutaneous hyperalgesia, cisplatin treatment might also induce muscle hyperalgesia associated with upregulations of P2X3 andASIC3. Interfering with these channels may prove to be a promising therapeutic target for treating painful sym... Neuromics news http://www.medworm.com/rss/comments.php?id=4355603 Sun, 16 Jan 2011 03:57:00 +0100 4355603 http://www.medworm.com/index.php?rid=4330805&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F01%2Fsox-antibodies-that-rock.html Our SOX Antibodies are proving to be excellent Stem Cell Markers.  I would like to highlight a pub that references use of our Sox17  and a variety of other markers:Pere Santamaria, Ignacio Rodríguez-Pizá, Xavier Clemente-Casares, Jun Yamanouchi, Lola Mulero-Perez, Trond Aasen, Angel Raya1 and Juan Carlos Izpisúa Belmonte. Turning Human Epidermis Into Pancreatic Endoderm. The Review of DIABETIC STUDIES 159 Vol. 7-No. 2-2010. DOI 10.1900/RDS.2010.7.158.This pub references use of a variety of markers for showing differentiation of Human embryonic stem (hES)and induced pluripotent stem (iPS)into into pancreatic endoderm structures. Real-time PCR and flow cytometry. A: Changes in the levels of different mRNAs in undifferentiated, and progressively differentiated, hES and iPS c... Neuromics news http://www.medworm.com/rss/comments.php?id=4330805 Tue, 11 Jan 2011 13:24:00 +0100 4330805 http://www.medworm.com/index.php?rid=4324755&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2011%2F01%2Fgreat-apoptosis-video.html Related Reagents:Apoptosis Research Reagents-Apoptosis Categories-includes: detection kits, antibodies and proteins (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4324755 Sat, 08 Jan 2011 14:31:00 +0100 4324755 http://www.medworm.com/index.php?rid=4277726&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F12%2Four-primary-hippocampal-cortical-and.html I would like to thank Dr. Lidia Gardner of University of Tennessee HSC for providing excellent images of cultures using our Combined Hippocampal, Cortical, and Ventricular Neurons. View Larger ImageHere's her feedback:  "I got 10 million cells total after extraction from the tissue.  At Day 4 they all developed long axons. Thank you so much for the replacement." Related Reagents:STEMEZ(TM) hN2 Human Neurons Discovery KitE18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURESE18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURESE18 Mouse Neuronal Tissue -NEURON CULTURESE18 Mouse Neuronal Tissue -ASTROCYTE CULTURESFrozen Primary Rat Cortical NeuronsHuman Brain Tissue (Blocks, Lysates and Slides)-NewGrowth Media, Poly-D-Lysine Coverslips and Fluoro-TracerFluoGreen TracerNeu... Neuromics news http://www.medworm.com/rss/comments.php?id=4277726 Tue, 21 Dec 2010 17:52:00 +0100 4277726 http://www.medworm.com/index.php?rid=4265190&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F12%2Fmarkers-for-medial-superior-olivary.html This study exclusively uses adult gerbils for in vitro electrophysiology, single-cell electroporation and immunohistochemistry to characterize the size and short-term plasticity of inputs to the MSO. We find that the excitatory and inhibitory inputs to the MSO are well balanced both in strength and short-term dynamics, redefining this fastest of all mammalian coincidence detector circuits. Related Reagents:Neuronal-Glial Markers-Astrocytes, Glia, Microglia, Olidogodendrocytes, Progenitors and Schwann Cell MarkersStem Cell Research AntibodiesStem Cell Research ReagentsPrimary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4265190 Thu, 16 Dec 2010 12:12:00 +0100 4265190 http://www.medworm.com/index.php?rid=4205805&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F11%2Ffragile-x-astrocytes-and-bmc-image-of.html Conclusions: These experiments are the first to establish a role for astrocytes in the delayed growth characteristics and abnormal morphological features in dendrites and synapses that characterize the Fragile X syndrome.Image: Co-culture of embryonic mouse hippocampal neurons and astrocytes. Primary embryonic hippocampal neurons at 7 days in vitro, were stained with Microtubule Associated Protein-2 (MAP, green) to enable the visualization of the dendritic arbors. These neurons were cultured on top of a monolayer of primary cortical astrocytes, stained with an antibody directed against Glial Fibrillary Acidic Protein (GFAP, red). The cell nuclei were visualized by staining with 4',6-diamidino-2-phenylindole (DAPI, blue). Related Links:Neuronal-Glial Markers-Astrocytes, Glia, Microglia, Oli... Neuromics news http://www.medworm.com/rss/comments.php?id=4205805 Sat, 27 Nov 2010 12:43:00 +0100 4205805 http://www.medworm.com/index.php?rid=4179079&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F11%2Fcancer-induced-bone-pain.html Bone crushing pain. This describes pain of the highest order. Our friend, Dr. Joseph Ghilardi, VAMC-Mpls. and his colleague, Dr. Patrick Manthy are finding the root causes of the intense and growing pain suffered by Cancer Victims. Here are highlights of a recent study:Pain frequently accompanies cancer. What remains unclear is why this pain frequently becomes more severe and difficult to control with disease progression. Here we test the hypothesis that with disease progression, sensory nerve fibers that innervate the tumor-bearing tissue undergo a pathological sprouting and reorganization, which in other nonmalignant pathologies has been shown to generate and maintain chronic pain. Injection of canine prostate cancer cells into mouse bone induces a remarkable sprouting of calcitonin gene... Neuromics news http://www.medworm.com/rss/comments.php?id=4179079 Thu, 18 Nov 2010 13:59:00 +0100 4179079 http://www.medworm.com/index.php?rid=4151312&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F11%2Four-trpv1-antibodies-rock.html We now have 40 publications referencing use of our TRP Antibodies in multiple applications. Here are the October-November 2010 publications referencing use of these antibodies:VR1 N-Terminus (TRPV1)-RabbitT. Wu, L. Song, X. Shi, Z. Jiang, J. Santos-Sacchi and A.L. Nuttal. Effect of capsaicin on potassium conductance and electromotility of guinea pig outer hair cell. doi:10.1016/j.heares.2010.10.010...anti-TRPV1 (rabbit polyclonal, RA10110, Neuromics, Edina, MN, USA) diluted to 1:500 with 1% BSA-PBS...antibody (TRPV-1) and its blocking peptide (104 M) (Neuromics, Edina, MN, USA)...(TRPV1) - mouse specificJulie A. Christianson, Klaus Bielefeldt, Sacha A. Malin and Brian M. Davis. Neonatal colon insult alters growth factor expression and TRPA1 responses in adult mice. Pain Volume 151, Is... Neuromics news http://www.medworm.com/rss/comments.php?id=4151312 Wed, 10 Nov 2010 14:10:00 +0100 4151312 http://www.medworm.com/index.php?rid=4118119&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F10%2Fvitamin-deficiency-and-hirschsprung.html Dr. Robert Heuckeroth and his team and Washington University recently published more results on the link between maternal Vitamin A Deficiency and Hirschsprung Disease. It underscores the importance of maternal vitamin A nutrition for preventing the diease penetrance and expressitivity:Ming Fu, Yoshiharu Sato, Ariel Lyons-Warren, Bin Zhang, Maureen A. Kane, Joseph L. Napoli and Robert O. Heuckeroth. Vitamin A facilitates enteric nervous system precursor migration by reducing Pten accumulation. Development 137, 631-640 (2010) doi:10.1242/dev.040550.SUMMARYHirschsprung disease is a serious disorder of enteric nervous system (ENS) development caused by the failure of ENS precursor migration into the distal bowel. We now demonstrate that retinoic acid (RA) is crucial for GDNF-induced ENS precu... Neuromics news http://www.medworm.com/rss/comments.php?id=4118119 Thu, 28 Oct 2010 23:02:00 +0100 4118119 http://www.medworm.com/index.php?rid=4097291&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F10%2Fstems-cells-and-sci.html This is fascinating and encouraging to sufferers of Spinal Cord Injury:ScienceDaily (Oct. 9, 2010) — Researchers at Karolinska Institutet have shown how stem cells, together with other cells, repair damaged tissue in the mouse spinal cord. The results are of potential significance to the development of therapies for spinal cord injury: http://www.sciencedaily.com/releases/2010/10/101008082736.htm# (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4097291 Thu, 21 Oct 2010 14:01:00 +0100 4097291 http://www.medworm.com/index.php?rid=4053225&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F10%2Fenhancing-neurite-outgrowth.html We are pleased to announce the addition of new proteins to our Axon Growth and Guidance category. These are designed to enhance neurite out growth. NameCatalog #TypeSpeciesSizePriceNGF-b, NSO DerivedPR15083ProteinH; M; R100 ug$285NGF-b, NSO Derived, CFPR15084CFProteinH; M; R100 ug$285S100A13, CFPR15085CF-50ProteinH; R50 ug$315Slit1PR15075-50ProteinCh; H50 ug$315Slit2, CHOPR15085-50ProteinCh; H50 ug$315Image: Cultured chick dorsal root ganglion neurons were grown in the presence of recombinant human NGF-b with (A) or without (B) recombinant mouse Slit2. The presence of the Slit2 protein signifi cantly enhanced neurite outgrowth. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=4053225 Sun, 10 Oct 2010 00:10:00 +0100 4053225 http://www.medworm.com/index.php?rid=3993429&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F09%2Fglutaredoxin-2-prevents-aggregation-of.html Our PTEN-induced kinase, PINK1 or PARK6 Antibody is an excellent marker for Amyotrophic Lateral Sclerosis (ALS) and Parkinson's Disease (PD) researchers.Here's  new publication referencing use of this antibody:  Alberto Ferri, Paolo Fiorenzo, Monica Nencini, Mauro Cozzolino, Maria Grazia Pesaresi, Cristiana Valle, Sara Sepe, Sandra Moreno, and Maria Teresa Carrì. Glutaredoxin 2 prevents aggregation of mutant SOD1 in mitochondria and abolishes its toxicity.Hum. Mol. Genet., first published on Sep 20, 2010 as doi: doi:10.1093/hmg/ddq383Abstract:Vulnerability of motoneurons in amyotrophic lateral sclerosis (ALS) arises from a combination of several mechanisms, including protein misfolding and aggregation, mitochondrial dysfunction and oxidative damage. Protein aggregates are found ... Neuromics news http://www.medworm.com/rss/comments.php?id=3993429 Wed, 22 Sep 2010 21:22:00 +0100 3993429 http://www.medworm.com/index.php?rid=3972655&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F09%2Ftrhr1-and-leprb-receptors-and.html This study focus on identifying loci in the hindbrain where leptin and TRH act synergistically to increase thermogenesis. Since thermogenic processes are at the root of how our bodies regulate energy, understanding the related expression and signaling pathways could be key to finding therapies for obesity.Maria J. Barnes, Richard C. Rogers, Montina J. Van Meter and Gerlinda E. Hermann. Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat. doi:10.1016/j.brainres.2010.07.094Example images: Distribution of LepRb+ fibers in hindbrain. LepRb-ir (red) fibers and varicosities are seen among TRHR1-ir (green) cells and fibers. These red and green fibers are adjacent and co-mingle but do not show co-localization of receptors. This pattern is seen in (A) ... Neuromics news http://www.medworm.com/rss/comments.php?id=3972655 Thu, 16 Sep 2010 00:44:00 +0100 3972655 http://www.medworm.com/index.php?rid=3968860&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F09%2Fisolation-of-medulloblastoma-stem-cells.html I am pleased to present this excellent video. It also has excellent images using key stem cell markers as the cells undergo differentiation:JoVE: Isolation, Enrichment, and Maintenance of Medulloblastoma ...Sep 1, 2010 ... GFAP antibody, Neuromics, CH22102, Chicken, 1:1000. Tuj1 antibody, Sigma, T5076, Mouse, 1:2000. NeuN antibody, Millipore, MAB377, Mouse, ...www.jove.com (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3968860 Tue, 14 Sep 2010 12:27:00 +0100 3968860 http://www.medworm.com/index.php?rid=3953825&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F09%2Fpotential-therapeutic-targets-for-bone.html Cancer pain is difficult to treat as it appears to be driven simultaneously by inflammatory, neuropathic and tumorigenic mechanisms. I have reported on multiple occasions publication referencing use of our Pain and Inflammation Research Antibodies in studying bone cancer pain. I would like to alert you to the latest reference:Timothy K. Y. Kaan, Ping K. Yip, Sital Patel, Meirion Davies, Fabien Marchand, Debra A. Cockayne, Philip A. Nunn, Anthony H. Dickenson, Anthony P. D. W. Ford, Yu Zhong, Marzia Malcangio, and Stephen B. McMahon Systemic blockade of P2X3 and P2X2/3 receptors attenuates bone cancer pain behaviour in rats. Brain, September 2010; 133: 2549 - 2564.......Slides were then incubated with rabbit anti-P2X3 (1:2000, Neuromics) and sheep anti-calcitonin gene-related peptide (1:100... Neuromics news http://www.medworm.com/rss/comments.php?id=3953825 Thu, 09 Sep 2010 22:14:00 +0100 3953825 http://www.medworm.com/index.php?rid=3844552&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F08%2Fangiogenesis-research-reagents.html The processes of angiogenesis and neurogenesis show striking similarities. Given our strong roots in providing Neuroscience research reagents, it is a natural extension for us to provide potent reagents for the studying the growth of new blood vessels.Angiogensis processes are important for healing wounds and for restoring blood flow to tissues after injury or insult. In females, it also occurs during the monthly reproductive cycle and during pregnancy(to build the placental circulation between mother and fetus.Images: Increased arteriogenesis and hyperemia in TSP2-null mice. Smooth muscle actin-positive and ephrin B2-positive vessels in WT (F, H) and TSP2-null (G, I). DOI: 10.2353/ajpath.2008.080128.Angiogenesis-dependent diseases result when new blood vessels either grow excessively or i... Neuromics news http://www.medworm.com/rss/comments.php?id=3844552 Sun, 08 Aug 2010 15:03:00 +0100 3844552 http://www.medworm.com/index.php?rid=3802164&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F07%2Fmicrornas-and-nociceptive-pain.html This study aimed to test whether let-7 microRNAs can regulate the µ opioid receptor (MOR) and opioid tolerance. Employing bioinformatics, we identified a let-7 binding site in the 3'-untranslated region (UTR) of MOR mRNA, which was experimentally confirmed as a direct target of let-7. The repressive regulation of MOR by let-7 was revealed using a LNA-let-7 inhibitor to knockdown let-7 in SH-SY5Y cells. Conversely, morphine significantly upregulated let-7 expression in SH-SY5Y cells and in a mouse model of opioid tolerance. The LNA-let-7 inhibitor decreased brain let-7 levels and partially attenuated opioid antinociceptive tolerance in mice. Although chronic morphine treatment did not change overall MOR transcript, polysome-associated mRNA declined in a let-7-dependent manner. let-7 was id... Neuromics news http://www.medworm.com/rss/comments.php?id=3802164 Thu, 29 Jul 2010 14:51:00 +0100 3802164 http://www.medworm.com/index.php?rid=3790480&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F07%2Fand-opioid-receptors-co-expression-and.html Dr. Tomas Hokfelt and his team at Karolinska Institute recently published use of our Opioid Receptor Antibodies and Substance P Antibody.They show the interplay of DOR and MOR in modulation of nociceptive afferent transmission and opioid analgesia. Hai-Bo Wanga, Bo Zhaoa, Yan-Qing Zhonga, Kai-Cheng Li, Zi-Yan Li, Qiong Wang, Yin-Jing Lua, Zhen-Ning Zhang, Shao-Qiu He, Han-Cheng Zheng, Sheng-Xi Wu, Tomas G. M. Hökfelt, Lan Baob, and Xu Zhanga. Coexpression of δ- and μ-opioid receptors in nociceptive sensory neurons. PNAS July 20, 2010 vol. 107 no. 29 13117-13122.Immunostaining. Adult rats, mice, and Oprd1 exon 1-deleted mice were fixed. Cryostat sections of L4 and L5 DRGs and spinal cord segments were processed for immunofluorescence staining (13) with Rb anti-DOR13–17 (1:2,000–1:60,... Neuromics news http://www.medworm.com/rss/comments.php?id=3790480 Mon, 26 Jul 2010 14:14:00 +0100 3790480 http://www.medworm.com/index.php?rid=3766952&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F07%2Fstaining-neuron-glial-cultures-related.html The objective being to find the right markers for a particular assay. I wanted to share examples of the potency of several Neurofilament or NF markers for labeling neurons:1. Neurofilament NF-L-Mouse Monoclonal Antibody (Clone: DA2) and Neurofilament alpha-internexin/NF66-Whole Serum-Rabbit AntibodyImages: Cells grown from adult rat brainLarge cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin (red). These cells were mitotic but had several characteristics of neurons. Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including N... Neuromics news http://www.medworm.com/rss/comments.php?id=3766952 Mon, 19 Jul 2010 20:15:00 +0100 3766952 http://www.medworm.com/index.php?rid=3729727&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F07%2Ftrpv1-p2x3-daily-double.html Conclusions/SignificanceLow pH leads to changes in several electrical properties of MSA, including initiation of ectopic action potentials which could propagate centrally but could also invade the peripheral endings causing glutamate release and activation of nearby nociceptors within the spindle capsule. This peripheral drive could contribute both to the transition to, and maintenance of, persistent muscle pain as seen in some “functional” pain syndromes.Images: Photomicrographs of trigeminal ganglion neurons stained with TRPV1 and P2X3.Related Reagents:Pain and Inflammation Research AntibodiesNeurotransmission Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3729727 Tue, 06 Jul 2010 18:46:00 +0100 3729727 http://www.medworm.com/index.php?rid=3706397&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F06%2Fotx2-orthodenticle-homeobox-2-and.html Our Otx2 Antibody is a potent marker for Human, Mouse and Rat Midbrain Dopamanergic Progenitors.This is confirmed by a recent publication by Dr. Ole Isaacson et al:Chee Yeun Chung, Pawel Licznerski, Kambiz N. Alavian, Antonio Simeone, Zhicheng Lin, Eden Martin, Jeffery Vance and Ole Isacson. The transcription factor orthodenticle homeobox 2 influences axonal projections and vulnerability of midbrain dopaminergic neurons. Brain Advance Access published online on June 23, 2010 Brain, doi:10.1093/brain/awq142... anti-Otx2 (Neuromics, 1:500)...Abstract: Two adjacent groups of midbrain dopaminergic neurons, A9 (substantia nigra pars compacta) and A10 (ventral tegmental area), have distinct projections and exhibit differential vulnerability in Parkinson’s disease. Little is known about transcr... Neuromics news http://www.medworm.com/rss/comments.php?id=3706397 Mon, 28 Jun 2010 11:37:00 +0100 3706397 http://www.medworm.com/index.php?rid=3699325&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F06%2Fnestin-as-marker-for-astrocytomas.html I recently highlighted the growing parade of pubs referencing use of our reagents for Cancer Research.I would like to add a new one. Angogenesis of Astrocytomas show stem like properties. This makes our Nestin Antibodies excellent markers.J H Tchaicha, A K Mobley, M G Hossain, K D Aldape and J H McCarty. A mosaic mouse model of astrocytoma identifies αvβ8 integrin as a negative regulator of tumor angiogenesis. Oncogene , (7 June 2010) doi:10.1038/onc.2010.199...chicken anti-Nestin IgY (Neuromics, Edina, MN, USA)...Abstract: Angiogenesis involves a complex set of cell–cell and cell–extracellular matrix (ECM) interactions that coordinately promote and inhibit blood vessel growth and sprouting. Although many factors that promote angiogenesis have been characterized, the identities and m... Neuromics news http://www.medworm.com/rss/comments.php?id=3699325 Fri, 25 Jun 2010 11:14:00 +0100 3699325 http://www.medworm.com/index.php?rid=3655504&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F06%2Fcancer-reagents-pubs-capabilities.html We continue to grow our capaibilities and abilities to serve Cancer Researchers.We recently highlighted the potency of our i-Fect ™ siRNA transfection kits for deliveriny siRNA to glioblastomas.Joseph George, Naren L. Banik, Swapan K. Ray. Combination of hTERT Knockdown and IFN-γ Treatment Inhibited Angiogenesis and Tumor Progression in Glioblastoma. Clin Cancer Res 2009;15(23):7186–95...with i-Fect transfection reagent (Neuromics) to obtain 5 μg DNA/10 μL of injection volume...Here're several new publications highlighting use of our Cancer Research Antibodies:Mauricio P. Pinto, Melanie M. Badtke, Michelle L. Dudevoir, J. Chuck Harrell, Britta M. Jacobsen and Kathryn B. Horwitz. Vascular Endothelial Growth Factor Secreted by Activated Stroma Enhances Angiogenesis and Hormone-Indepen... Neuromics news http://www.medworm.com/rss/comments.php?id=3655504 Fri, 11 Jun 2010 18:20:00 +0100 3655504 http://www.medworm.com/index.php?rid=3633398&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F06%2Fstemeztm-hnp1-cells-and-neuroprotection.html The objectives of this study were to determine if spirulina could protect the proliferative potential of hippocampal neural progenitor cells from an acute systemic inflammatory insult of lipopolysaccharide (LPS). To this end, young rats were fed for 30 days a control diet or a diet supplemented with 0.1% spirulina. On day 28 the rats were given a single i.p. injection of LPS (1 mg/kg). The following day the rats were injected with BrdU (50 mg/kg b.i.d. i.p.) and were sacrificed 24 hours after the first injection of BrdU. Quantification of the BrdU positive cells in the subgranular zone of the dentate gyrus demonstrated a decrease in proliferation of the stem/progenitor cells in the hippocampus as a result of the LPS insult. Furthermore, the diet supplemented with spirulina was able to nega... Neuromics news http://www.medworm.com/rss/comments.php?id=3633398 Sun, 06 Jun 2010 11:33:00 +0100 3633398 http://www.medworm.com/index.php?rid=3611858&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Fstemeztm-hnp1-human-neural-progenitors.html CONCLUSIONS• hES cells displayed the widest array of GPCR transcripts, while neural progenitors displayed the most restricted population.• The Frizzled (FZD) family of receptors were among the most abundantly expressed transcripts across all populations.• Neural progentitors up-regulated GPCR transcripts important to brain angiogenesis, cell proliferation, neurogenesis and cell adhesion.• Further differentiated hN2 cells displayed up-regulation of a wider population of transcripts including GPCRs involved with neurotransmission.• Functional assays demonstrated responses to sphingosine-1-phosphate in both hNP1 and hN2 populations of cells.• hES cells and their derived tissue provide a unique model to study endogenous GPCR signaling in non-transformed cells for drug screening app... Neuromics news http://www.medworm.com/rss/comments.php?id=3611858 Sat, 29 May 2010 20:48:00 +0100 3611858 http://www.medworm.com/index.php?rid=3599260&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Fdr-steve-stice-to-present-power-of.html Posted using ShareThisI have profiled Steve Stice's research here. The focus has been the excellent research results he and his team at ArunA Biomedical have generated with STEMEZ(TM) hN2 Human Neurons and hNP1 Human Neural Progenitors.The story continues. He will be presenting the latest at the 9th Annual World Pharmaceutical Congress in Philadelphia, June 14. Topics include: using these neural cell lines to study neurotoxicity in cell-based assays and disease modeling. Recent work conducted in outside laboratories demonstrates that these lines are more sensitive to environmental toxicants than traditional cellular models.Sample high throughput assay applications:Cell morphology and neurite outgrowthCell signaling and transcription factor expressionReceptor and ion channel functionCytotox... Neuromics news http://www.medworm.com/rss/comments.php?id=3599260 Wed, 26 May 2010 00:09:00 +0100 3599260 http://www.medworm.com/index.php?rid=3585310&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Flabeling-kits-tags-secondary-antibodies.html Labeling kits, tags, secondary antibodies and related reagentsPosted using ShareThisLabeling and tagging is an important step in your research process. This often drives the wow factor in published results. We offer some of the best and brightest including:CHROMEOTMsity-exhibit superior luminescence properties,including a broad range of fluorescence excitation and emission, largeStokes shifts, limited photobleaching and a broad pH tolerance.ELISA Buffers and Diluents Solulink™ Labeling Kits and Beads-The most efficient labeling kits delivering ready-to-use conjugates for the novice or the expert!Cytoplasmic and Nuclear StainingStrep-Tag®-One-STrEP-tag for protein complex purification.Image: CHROMEOsity 488: HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo... Neuromics news http://www.medworm.com/rss/comments.php?id=3585310 Fri, 21 May 2010 13:58:00 +0100 3585310 http://www.medworm.com/index.php?rid=3569675&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Fapoptosis-signaling-visualization-and.html Apoptosis-Oxidative Stress Research Reagents are widely used and frequently referenced in customer publications. We work hard to keep our fingers on the pulse of how they are utilized across the many research areas important to our customers and add new reagents based on evolving requirements.I recently posted publications referencing our MitoPTTM Kits for quantitating Tumor ApoptosisNew Pub referencing Polycaspase Assay Kit, green: L. Wei, D. Ding and R. Salvi. Salicylate-induced degeneration of cochlea spiral ganglion neurons-apoptosis signaling. doi:10.1016/j.neuroscience.2010.03.015. Images: Typical confocal photomicrographs of SGN stained with Polycaspase Assay Kit (green) and with an antibody against neuronal III ß-tubulin (red) to identify SGN. (A) In control cultures, most SGN hav... Neuromics news http://www.medworm.com/rss/comments.php?id=3569675 Sun, 16 May 2010 16:27:00 +0100 3569675 http://www.medworm.com/index.php?rid=3567859&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Fangiogensis-and-cd-antigens.html We consider everything genesis as a core focus area. This includes embryogenesis, angiogenesis, neurogenesis and stem cell expansion and differentiation. This is important because there is an intersection between reagents and methods for studying embryo development and genesis pathways in "adult systems".CD antigens serve as markers for growth and development. We are always on the look out for Customer Publications that reference use of these reagents in related applications. Here's one that just pinged our radar.Karim Harhouri, Abdeldjalil Kebir, Benjamin Guillet, Alexandrine Foucault-Bertaud, Serge Voytenko, Marie-Dominique Piercecchi-Marti, Caroline Berenguer, Edouard Lamy, Frédéric Vely, Pascale Pisano, L'Houcine Ouafik, Florence Sabatier, José Sampol, Nathalie Bardin, Françoise Di... Neuromics news http://www.medworm.com/rss/comments.php?id=3567859 Sat, 15 May 2010 18:26:00 +0100 3567859 http://www.medworm.com/index.php?rid=3546811&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F05%2Ftrpv1-expression-and-temporal-lobe.html This study utilized in vitro electrophysiological methods to examine the effect of transient receptor potential vanilloid type 1 (TRPV1) activation in dentate gyrus granule cells in a murine model of TLE. Capsaicin, a selective TRPV1 agonist had no measurable effect on overall synaptic input to granule cells in control animals, but significantly enhanced spontaneous and miniature EPSC frequency in mice with TLE. Exogenous application of anandamide, an endogenous cannabinoid that acts at both TRPV1 and cannabinoid type 1 receptors (CB1R), also enhanced glutamate release in the presence of a CB1R antagonist. Anandamide reduced the EPSC frequency when TRPV1 were blocked with capsazepine. Western blot analysis of TRPV1 receptor indicated protein expression was significantly greater in the dent... Neuromics news http://www.medworm.com/rss/comments.php?id=3546811 Sat, 08 May 2010 19:33:00 +0100 3546811 http://www.medworm.com/index.php?rid=3501436&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F04%2Fmitopt-for-studying-tumor-apoptosis.html We value our partnership with ICT. They provide our customers with potent and research proven Apoptosis Kits and Methods. Here we feature publications referencing our MitoPT™ Kits. These Kits easily assess changes in mitochondrial membrane potential. Changes in mitochondrial membrane potential can correlate with cytochrome c release and the initiation of apoptosis. A431 cells, treated with predetermined IC50concentration of novel anticancer agents, fluoresce green and orange-red with MitoPT JC-1. Data courtesy of Zayas/ Carro, Universidad Metropolitana.Anticancer Effects of Alpinia pricei Hayata Roots.CL Hsu, YS Yu, GC Yen. J. Agric. Food Chem., Jan 2010, 58 (4), pp 2201–2208.Anticancer Effects of Flavonoid Derivatives Isolated from Millettiareticulata Benth in SK-Hep-1 Human Hepatocel... Neuromics news http://www.medworm.com/rss/comments.php?id=3501436 Sat, 24 Apr 2010 14:27:00 +0100 3501436 http://www.medworm.com/index.php?rid=3490457&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F04%2Fcfr1-5-ht2ar-and-anxiety-behavior.html We have a potent offering of 5HT-Serotonin Antibodies. This is confirmed by our growing parade of customer publications referencing their use.We are pleased to present a new publication referencing use of our 5HT (Serotonin) 2A Receptor Antibody. Dr. Stephen S G Ferguson and team have discovered a link between CFR1 and 5-HT2A Receptor expression:Ana C Magalhaes,Kevin D Holmes,Lianne B Dale,Laetitia Comps-Agrar,Dennis Lee,Prem N Yadav, Linsay Drysdale, Michael O Poulter, Bryan L Roth, Jean-Philippe Pin, Hymie Anisman& Stephen S G Ferguson. CRF receptor 1 regulates anxiety behavior via sensitization of 5-HT2 receptor signaling. Nature Neuroscience. doi:10.1038/nn.2529. Published online11 April 2010.Abstract: Stress and anxiety disorders are risk factors for depression and these behaviors... Neuromics news http://www.medworm.com/rss/comments.php?id=3490457 Tue, 20 Apr 2010 18:26:00 +0100 3490457 http://www.medworm.com/index.php?rid=3463377&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F04%2Fmore-on-neuromics-neuron-markers.html I have multiple posts on the potency of our Neuron Markers. I am pleased to present yet another reference. This on features use of our Chicken Tyrosine Hydroxylase-TH antibody. It features staining of juxtaglomerular cells in the olfactory bulb of mice:Hans-Ulrich Fried, U. Benjamin Kaupp and Frank Müller. Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice. Cell Tissue Res. 2010 March; 339(3): 463–479. Published online 2010 February 6. doi: 10.1007/s00441-009-0904-9.Image: TH antibody staining in ET-like cell populations within the Glomerulari (GL). Dilution 1:500Related Reagents: TH-Mouse Monoclonal Catalog#: MO20001TH-Monoclonal-High Titer Catalog#:MO22941 All Neuron-Glial MarkersNeurodeg... Neuromics news http://www.medworm.com/rss/comments.php?id=3463377 Tue, 13 Apr 2010 12:17:00 +0100 3463377 http://www.medworm.com/index.php?rid=3411034&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F03%2Fpotent-neuron-glial-markers.html We are recognized for having top shelf Neuron/Glial Marker Antibodies. We have an extensive catalog and have customer referencing use of these in a variety of applications, species and cell types.Cell types include neural progenitors, neurons, glia, astrocytes, schwann cells and more. We are pleased to provide present a new publication referencing use of our MAP2 (Microtubule assoc. protein 2) Antibody for immunostaining of E17 primary mouse astrocytes.Shelley Jacobs and Laurie C. Doering. Astrocytes Prevent Abnormal Neuronal Development in the Fragile X Mouse. J. Neurosci., Mar 2010; 30: 4508 - 4514 ; doi:10.1523/JNEUROSCI.5027-09.2010.After 7 d in vitro (DIV), the cells were fixed with ice-cold (–20°C) methanol and processed for immunocytochemistry. After the appropriate serum block, ... Neuromics news http://www.medworm.com/rss/comments.php?id=3411034 Fri, 26 Mar 2010 19:16:00 +0100 3411034 http://www.medworm.com/index.php?rid=3398734&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F03%2Fprimary-hypothalamic-neurons-cultures.html Our customers have had great success dissociating and culturing our Fresh E18 and E20Rat Primary Neuronal Tissue. They have proven useful for a variety of cell based assays.Here're several publications referencing use of the neurons: Lisette T. Arnaud, Natura Myeku and Maria E. Figueiredo-Pereira. Proteasome–caspase–cathepsin sequence leading to tau pathology induced by prostaglandin J2 in neuronal cells. Journal of Neurochemistry. Volume 110 Issue 1, Pages 328 - 342 .Karunya K. Kandimalla1, Olenych G. Scott, Smita Fulzele1, Michael W. Davidson, Joseph F. Poduslo. Mechanism of Neuronal versus Endothelial Cell Uptake of Alzheimer's Disease Amyloid β Protein. PLoS ONE 4(2): e4627. doi:10.1371/journal.pone.0004627.The most challenging of our neurons to culture are from the microdissect E... Neuromics news http://www.medworm.com/rss/comments.php?id=3398734 Tue, 23 Mar 2010 23:28:00 +0100 3398734 http://www.medworm.com/index.php?rid=3378135&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F03%2Fnew-human-mesenchymal-stem-cells.html Neuromics' is responding to growing demand for Stem Cell Research Reagents.This includes the addition of human stem cells giving researchers the ability to create consistent cultures of primary cells. Our first offering was STEMEZ TM hNP1 Human Neural Progenitors. We have received positive feedback on these regarding ease of use and quality.We are pleased to announce the addition of Human Mesenchymal Stem Cells.Image: Pancreas-derived human mesenchymal stem cells labeled with a CD44 monoclonal antibody conjugated to fluorescein isothiocyanate (FITC), which detects the CD44 cell surface protein.They are isolated from human adult pancreas and can be induced to differentiate into beta-cells, which is a significant product in the diabetes research area. MSCs to Beta Cells Protocol.This is extr... Neuromics news http://www.medworm.com/rss/comments.php?id=3378135 Thu, 18 Mar 2010 14:57:00 +0100 3378135 http://www.medworm.com/index.php?rid=3363604&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F03%2Fneuropeptides-and-large-dense-core.html We continue to be recognized by for the quantity and quality of our Neuropeptide and Neuropeptide Receptor Antibodies for studying Neurotransmission and Pain.We wanted to feature an new article referencing use of our Guinea Pig Substance Antibody. Dr. Richard Mains and his team shed light on the function and behavior of large dense corevesicles (LDCVs) concluding that under basal conditions, LDCVs move faster away from the soma than toward the soma, but fewer LDCVs travel anterograde than retrograde. Stimulation decreased average anterograde velocity and increases granule pausing. Data from antibody uptake, quantification of enzyme secretion and appearance of pHluorin fluorescence demonstrate distributed release of peptides all along the axon, not just at terminals. Jacqueline A Sobota , W... Neuromics news http://www.medworm.com/rss/comments.php?id=3363604 Sat, 13 Mar 2010 18:34:00 +0100 3363604 http://www.medworm.com/index.php?rid=3339607&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F03%2Fvitamin-and-hirschsprung-disease.html In this study, he and his team find a link between Vitamin A and the disease. This suggests that some cases of Hirschsprung disease might be preventable by optimizing maternal nutrition.Ming Fu, Yoshiharu Sato, Ariel Lyons-Warren, Bin Zhang, Maureen A. Kane, Joseph L. Napoli, and Robert O. Heuckeroth Vitamin A facilitates enteric nervous system precursor migration by reducing Pten accumulation. Development, Feb 2010; 137: 631 - 640. Featured Reagent: Ret Related Reagents:Ret BiotinylatedRet (C-Terminus Fused)Ret-Fluorescein LabeledRet-Allophycocyanin LabeledRet-Phycoerythrin LabeledNeurotrophins and Growth Factor AntibodiesNeurotrophins-Neuron/Glial Marker Recombinant ProteinsNeuron/Glial MarkersStem Cell Research Reagents (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3339607 Sat, 06 Mar 2010 22:57:00 +0100 3339607 http://www.medworm.com/index.php?rid=3287513&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F02%2Fneuromics-pain-research-customers.html Neuromics' Pain Research Customers continue to make gains using our Pain and Inflammation Research Antibodies and Transfection Kits. Here are the latest pubs:Hua Zhang and A. S. Verkman. Aquaporin-1 Tunes Pain Perception by Interaction with Nav1.8 Na+ Channels in Dorsal Root Ganglion Neurons. February 19, 2010 The Journal of Biological Chemistry, 285, 5896-5906....chicken anti-calcitonin gene-related peptide (CGRP; 1:500, Neuromics, Edina, MN)...Nathaniel A. Sowa, Bonnie Taylor-Blake, and Mark J. Zylka. Ecto-5'-Nucleotidase (CD73) Inhibits Nociception by Hydrolyzing AMP to Adenosine in Nociceptive Circuits. The Journal of Neuroscience, February 10, 2010, 30(6):2235-2244; doi:10.1523/JNEUROSCI.5324-09.2010. ...rabbit anti-P2X3-RA10109, Neuromics; 1:750), rabbit anti- VR1 C-Terminus (TRPV1) ... Neuromics news http://www.medworm.com/rss/comments.php?id=3287513 Fri, 19 Feb 2010 15:46:00 +0100 3287513 http://www.medworm.com/index.php?rid=3258694&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F02%2Fturning-placenta-into-brain.html ConclusionStem cells derived from human placenta can be differentiated into neural progenitors. Featured AntibodyTuj 1 (Neuron-specific class III beta-tubulin)-Mouse Related Reagents:NSE (Neuron-Specific Enolase)NestinMusashi-1Neuron/Glial MarkersStem Cell Research Reagents (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3258694 Tue, 09 Feb 2010 19:16:00 +0100 3258694 http://www.medworm.com/index.php?rid=3216398&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F01%2Fnucleostemin-stem-cell-marker.html Our Nucleostemin Antibody has proven a versatile Stem Cell Marker.Researchers have referenced use of this antibody as a marker for Neural Progenitors and Muscle-Derived Stem Cells. In the most recent of our Nucleostemin Publications, Dr James Wang (University of Pittsburgh) references using the antibody for staining Tendon Progenitors/Stem Cells (TSCs):Jianying Zhang and James H-C. Wang. Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskeletal Disorders. 2010, 11:10doi:10.1186/1471-2474-11-10....The staining protocol used goat anti-human Nucleostemin Antibody (1:300; Neuromics, Cat. No. GT15050) and Cy3-conjugated donkey anti-goat IgG secondary antibody...Image: Achilles Tendon Stem Cells (ATSCs) expressed nucleostemin. Insets show enlarged... Neuromics news http://www.medworm.com/rss/comments.php?id=3216398 Thu, 28 Jan 2010 00:42:00 +0100 3216398 http://www.medworm.com/index.php?rid=3185122&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2010%2F01%2Fwe-are-for-reagents-that-brighten-your.html We are all for reagents that brighten your day.i-BRITE Plus is a glycerol based liquid. It can be used to stain cells and can be easily added to wells. It also will not shrink tissue. In addition standard to IHC/IF applications, it can be used to visualize GFP transfections and more.Here's a publication referencing it: Ajay S. Yekkirala, Alexander E. Kalyuzhny and Philip S. Portoghese. Standard Opioid Agonists Activate Heteromeric Opioid Receptors: Evidence for Morphine and [d-Ala2-MePhe4-Glyol5]Enkephalin as Selective μ−δ Agonists. ACS Chem. Neurosci., Article ASAP DOI: 10.1021/cn9000236. Publication Date (Web): November 25, 2009. Copyright © 2009 American Chemical Society.After that cells were washed in PBS (3 × 15 min), counterstained with DAPI and mounted under coverslips with an... Neuromics news http://www.medworm.com/rss/comments.php?id=3185122 Tue, 19 Jan 2010 12:23:00 +0100 3185122 http://www.medworm.com/index.php?rid=3129201&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F12%2Ftuj-1-neuronal-differentiation-marker.html Our Tuj-1 antibodies are widely used and frequently referenced in customer publications. They are proven markers for Neural Progenitor and Neuronal Differentiation. Here's the latest reference:Alonso M. Higuero, Lucía Sánchez-Ruiloba1, Laura E. Doglio, Francisco Portillo1, José Abad- Rodríguez, Carlos G. Dotti and Teresa Iglesias. Kidins220/ARMS modulates the activity of microtubule-regulating proteins and controls neuronal polarity and development. JBC Papers in Press. Published on November 10, 2009 as Manuscript M109.024703....Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse (MO15013, Neuromics Antibodies, Edina, MN)...Immunofluorescence Method:Cells grown on coverslips were fixed for 5 min in 4% paraformaldehydecontaining 4% sucrose in phosphate buffer saline (PBS) at 37ºC. Cel... Neuromics news http://www.medworm.com/rss/comments.php?id=3129201 Wed, 30 Dec 2009 00:22:00 +0100 3129201 http://www.medworm.com/index.php?rid=3118731&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F12%2Fopioid-receptor-and-depression.html Tricyclic antidepressants (TCAs) have been reported to interact with the Opioid Receptor system, but their pharmacological activity at opioid receptors has not yet been elucidated.We would like to share a recent publications that sheds more light on the mystery. Our purified rabbit ployclonal phosphoERK1/2 Antibody is also referenced.Pierluigi Onali, Simona Dedoni and Maria C. Olianas. Direct Agonist Activity of Tricyclic Antidepressants at Distinct Opioid Receptor Subtypes. JPET January 2010 vol. 332 no. 1 255-265 .At the cloned μ-opioid receptor, TCAs showed low affinity and no significant agonist activity. These results show that TCAs differentially regulate opioid receptors with a preferential agonist activity on either δ or κ subtypes and suggest that this property may contribute t... Neuromics news http://www.medworm.com/rss/comments.php?id=3118731 Thu, 24 Dec 2009 14:51:00 +0100 3118731 http://www.medworm.com/index.php?rid=3079088&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F12%2Fhope-for-stroke-victims-transplanting.html In a recent publication (Jen et al., 2009) Neuromics'/ArunA’s STEMEZTM human neural progenitor (hNP1) cells when injected (sterotaxic) into a rat stroke model produced significant beneficial results. The hNP1 cells reduced the infarct area by 50% and were positive for neuronal marker proteins, cleaved caspase-3 and 40% of the cells showed spontaneous action potentials and excitatory postsynaptic currents measured by patch clamp recordings at 8 weeks post hNP1 cell injections. The treated rats had improves cognitive and sensorimotor functions between four to nine months post injection. These Sprague–Dawley rats were not immunosuppressed.Kunlin Jin, XiaoOu Mao, Lin Xie, Veronica Galvan, Bin Lai, Yaoming Wang, Olivia Gorostiza, Xiaomei Wang and David A Greenberg. Transplantation of human ... Neuromics news http://www.medworm.com/rss/comments.php?id=3079088 Fri, 11 Dec 2009 11:40:00 +0100 3079088 http://www.medworm.com/index.php?rid=3070973&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F12%2Fgfap-antibody.html Check it out!GFAP AntibodyExcelent marker for human astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.Posted using ShareThis (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3070973 Wed, 09 Dec 2009 14:33:00 +0100 3070973 http://www.medworm.com/index.php?rid=3061377&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F12%2Fblood-pressure-transient-receptor.html Dr. Dr. Hui-Lin Pan (Department of Anesthesiology) and his team at M. D. Anderson Cancer Center has been a loyal user of our TRPV1 (VR1) since the early days of Neuromics' existence. We appreciate their continuing to use our reagent in their research.Here's yet another publication:Hao Sun, De-Pei Li, Shao-Rui Chen, Walter N. Hittelman and Hui-Lin Pan. Sensing of Blood Pressure Increase by Transient Receptor Potential Vanilloid 1 Receptors on Baroreceptors. doi:10.1124/jpet.109.160473...VR1 C-terminus (TRPV1), dilution 1:1000, Neuromics...Related Reagents:All TRP AntibodiesPain and Inflammation Research AntibodiesNeurotransmission -Neurotransmission Research Antibody Categories (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=3061377 Sat, 05 Dec 2009 17:28:00 +0100 3061377 http://www.medworm.com/index.php?rid=2992571&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F11%2Fnpy-y2r-and-ihc-mouse-distal-colon.html Lixin Wang, Guillaume Gourcerol, Pu-Qing Yuan, S. Vincent Wu, Mulugeta Million, Muriel Larauche, and Yvette Taché. Peripheral peptide YY inhibits propulsive colonic motor function through Y2 receptor in conscious mice. Am J Physiol Gastrointest Liver Physiol (November 5, 2009). doi:10.1152/ajpgi.00349.2009. ...Note: Excellent IHC staining of myenteric plexus and submucosal (mouse distal colon) tissue-Free floating submucosal and LMMP whole mounts of both proximal and distal colon from 3 naïve mice were treated in 10% normal goat serum each for 30 min, and followed by incubation with polyclonal rabbit anti- NPY Y2 Receptor diluted at 1:1,000 (Neuromics, Inc., Edina, MN)...Related Antibodies to Consider:NPY Y2 Receptor-C/N TerminusNPY Y1 ReceptorppNPYAll Neuropeptide and Neuropeptide Recep... Neuromics news http://www.medworm.com/rss/comments.php?id=2992571 Fri, 13 Nov 2009 21:25:00 +0100 2992571 http://www.medworm.com/index.php?rid=2988894&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F11%2Fhttpneuromics2009a21berylliumittriumcom.html Jakob S. Satz, Alisdair R. Philp, Huy Nguyen, Hajime Kusano, Jane Lee, Rolf Turk, Megan J. Riker, Jasmine Hernández, Robert M. Weiss, Michael G. Anderson, Robert F. Mullins, Steven A. Moore, Edwin M. Stone, and Kevin P. Campbell. Visual Impairment in the Absence of Dystroglycan. J. Neurosci., Oct 2009; 29: 13136 - 13146 ; doi:10.1523/JNEUROSCI.0474-09.2009. ....anti-mGluR6 (Neuromics)...Lasani S. Wijetunge, Sally M. Till, Thomas H. Gillingwater, Cali A. Ingham, and Peter C. Kind. mGluR5 Regulates Glutamate-Dependent Development of the Mouse Somatosensory Cortex. The Journal of Neuroscience, December 3, 2008, 28(49):13028-13037; doi:10.1523/JNEUROSCI.2600-08.2008....Western blotting was performed as mentioned above and membranes were probed with antibodies against mGluR5 (1:4000, Neuromics... Neuromics news http://www.medworm.com/rss/comments.php?id=2988894 Fri, 13 Nov 2009 15:58:00 +0100 2988894 http://www.medworm.com/index.php?rid=2970143&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F11%2Fgp130-il-6-and-expression-and.html We wanted to post yet another publication referencing use of one of our Pain and Inflammation Research Antibodies. Here the researchers used our Mouse Monoclonal gp130/CD130 for Immunohistochemistry.Manfred Andratsch, Norbert Mair, Cristina E. Constantin, Nadja Scherbakov, Camilla Benetti, Serena Quarta, Christian Vogl, Claudia A. Sailer, Nurcan Üceyler, Johannes Brockhaus, Rudolf Martini, Claudia Sommer, Hanns Ulrich Zeilhofer, Werner Müller, Rohini Kuner, John B. Davis, Stefan Rose-John, and Michaela Kress. A Key Role for gp130 Expressed on Peripheral Sensory Nerves in Pathological Pain. J. Neurosci., Oct 2009; 29: 13473 - 13483 ; doi:10.1523/JNEUROSCI.1822-09.2009The results suggest that gp130 expressed in sensory nerves not only mediates chronic inflammatory pain, but also contribute... Neuromics news http://www.medworm.com/rss/comments.php?id=2970143 Fri, 06 Nov 2009 22:07:00 +0100 2970143 http://www.medworm.com/index.php?rid=2954304&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F11%2Freagents-that-work-for-drug-discovery.html We have redesigned our website! New capabilities easier for visitors to find products and related product content:Reagents that work for Drug Discovery and Basic Research.Posted using ShareThis (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2954304 Mon, 02 Nov 2009 23:18:00 +0100 2954304 http://www.medworm.com/index.php?rid=2908439&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F10%2Fnse-and-tuj-1-and-parkinsons-disease.html I would like to thank Meghan Coakley from University College Cork for alerting me to a new publication referencing our Chicken NSE (Neuron-Specific Enolase) and Tuj 1 (Neuron-specific class III beta-tubulin) antibodies.Here's her feedback: "Just letting you know we published our paper using the beta-III-Tubulin and NSE antibodies you supplied to us – Timmons et al., Neuroscience Letters, Oct 1, 2009 [Epub ahead of print]. The antibodies were excellent and I’m sure we’ll be using Neuromics again in the future."Timmons S, Coakley MF, Moloney AM, O' Neill C. Akt signal transduction dysfunction in Parkinson's disease. Neurosci Lett. 2009 Oct 1. [Epub ahead of print].Abstract: Significant attention has been drawn to the potential role of defective PI3-kinase-Akt (PKB) signalling in Parkin... Neuromics news http://www.medworm.com/rss/comments.php?id=2908439 Mon, 19 Oct 2009 20:04:00 +0100 2908439 http://www.medworm.com/index.php?rid=2902738&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F10%2Fmore-success-with-trpv1.html Dr. Irmgard Tegeder and team have been using our VR1 N-Terminus (TRPV1) as a marker to study how B kinase (IKK) plays a role in sensory neuron excitability and nociception.The antibody was used for both Western Blot and Immunohistochemistry. Here's a recent publication of their results:Vanessa Bockhart, Cristina Elena Constantin, Annett Häussler, Nina Wijnvoord, Maike Kanngiesser, Thekla Myrczek, Geethanjali Pickert,Laura Popp, Jürgen-Markus Sobotzik, Manolis Pasparakis, Rohini Kuner, Gerd Geisslinger, Christian Schultz, Michaela Kress, and Irmgard Tegeder. Inhibitor B Kinase β Deficiency in Primary Nociceptive Neurons Increases TRP Channel Sensitivity. The Journal of Neuroscience, October 14, 2009, 29(41):12919-12929; doi:10.1523/JNEUROSCI.1496-09.2009Related Reagents:All TRPV (Vanillo... Neuromics news http://www.medworm.com/rss/comments.php?id=2902738 Sat, 17 Oct 2009 16:09:00 +0100 2902738 http://www.medworm.com/index.php?rid=2875777&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F10%2Fmmp-9-squared.html We are feverishly working on adding reagents for studying Autoimmunity and Immune Response. In this context, we wanted to alert you to 2 new publications referencing our Matrix Metalloproteinase 9 (MMP-9) antibody.Zhi-Yuan Zhang, Zhiren Zhang, Caroline Zug, Barbara Nuesslein-Hildesheim, David Leppert and Hermann J. Schluesener. AUY954, a selective S1P1 modulator, prevents experimental autoimmune neuritis. doi:10.1016/j.jneuroim.2009.09.010.Abstract:Experimental autoimmune neuritis (EAN) is a T cell-mediated autoimmune inflammatory demyelinating disease of the peripheral nervous system and an animal model of human inflammatory demyelinating polyradiculoneuropathy. AUY954, which targets selectively the sphingosine-1-phosphate receptor 1 (S1P1), is known to sequester lymphocytes into secondar... Neuromics news http://www.medworm.com/rss/comments.php?id=2875777 Fri, 09 Oct 2009 14:26:00 +0100 2875777 http://www.medworm.com/index.php?rid=2851467&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F09%2Frock-solid-tuj-1.html The feedback from customers on our Tuj 1 (Neuron-specific class III beta-tubulin) is that it works!This is confirmed by the growing list of references in key publications. Here's the latest:S A Sakowski, S B Heavener, J S Lunn, K Fung, S S Oh, S K Spratt, N D Hogikyan and E L Feldman. Neuroprotection using gene therapy to induce vascular endothelial growth factor-A expression. Gene Therapy advance online publication 3 September 2009; doi: 10.1038/gt.2009.111....TUJ1 (Neuromics, Edina, MN, USA). ...Tuj 1 (Neuron-specific class III beta-tubulin)Related Reagents:NestinMusashi-1Other Reagents to Consider:Stem Cell ReagentsNeuron/Glial Markers (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2851467 Wed, 30 Sep 2009 21:58:00 +0100 2851467 http://www.medworm.com/index.php?rid=2842384&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F09%2Fprodynorphin-at-work.html We would like to Dr. Andrew Todd for sharing this excellent IHC image using our Guinea Pig ProDynorphin (rat) antibody. Image: Staining of adult rat spinal cord.The tissue is perfusion-fixed (4% freshly prepared formaldehyde) adult rat spinal cord, reacted overnight with the PPD at 1:1000 and then o/n in Alexa488 secondary (raised in donkey, Invitrogen, 1:500).The confocal image stack was taken through a 60x oil lens (Bio-Rad Radiance confocal) - pixel size is 0.196 micrometre and this is a projection of 10 confocal optical sections at 0.5 micrometre z-spacing.Customer PublicationsRelated Reagents:proDynorphin (guinea pig)Opioid ReceptorsPain and Inflammation Antibodies (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2842384 Mon, 28 Sep 2009 19:26:00 +0100 2842384 http://www.medworm.com/index.php?rid=2803779&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F09%2Fcamkiis-and-neurotransmitters.html Here's an update on recent developments. First, we wanted to share a recent publication referencing 2 of our Neurotransmission Research Antibodies:Haifeng Zhaoa, Qiong Li, Xinrong Pei, Zhaofeng Zhang, Ruiyue Yang, Junbo Wang and Yong Li. Long-term ginsenoside administration prevents memory impairment in aged C57BL/6J mice by up-regulating the synaptic plasticity-related proteins in hippocampus. Behavioural Brain Research Volume 201, Issue 2, 12 August 2009, Pages 311-317. doi:10.1016/j.bbr.2009.03.002. ...anti-phospho-CaMKII (Thr286) and anti- CaMKII (Neuromics, USA)...Second, we continue to bulk up on reagents for Neurotransmission, Neuromodulation and Synaptic Plasticity Research Reagents. Check out these product categories:Biogenic AminesCalcium SignalingG-Protein Coupled ReceptorsLigan... Neuromics news http://www.medworm.com/rss/comments.php?id=2803779 Wed, 16 Sep 2009 22:01:00 +0100 2803779 http://www.medworm.com/index.php?rid=2785714&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F09%2Ftrpv1-antibodies-in-action.html We would like to thank Dr. Fletcher White, LUMC, for Sharing this data with us:Images: The CCR2 chemokine receptor colocalized with IB4 and TRPV1, markers of nociceptive neurons, after injury. A) Many lumbar DRG neurons in vehicle-treated rat sensory neurons were positive for IB4, a neuronal phenotype that distinguishes some C-fiber nociceptors (red cells), however there was no expression of the CCR2 protein. B) After perineural gp120/hCD4 treatment, CCR2 protein expression (green cells) was upregulated, and co-localized with IB4. C) Both gp120/hCD4 and ddC treatment resulted in an upregulation of CCR2 expression (green cells) in many small and medium diameter neurons. Again, CCR2 co-localized in a number of IB4 positive cells. D) The TRPV1 channel is present on many nociceptive neurons an... Neuromics news http://www.medworm.com/rss/comments.php?id=2785714 Thu, 10 Sep 2009 18:43:00 +0100 2785714 http://www.medworm.com/index.php?rid=2743926&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Ftracking-stemez-hnp1-progenitor-cell.html We would like to promote an important new discovery using our STEMEZ(TM) hNP1 Human Neural Progenitors Discovery Kit.Human embryonic stem cell–derived neural progenitors (NP) present an important tool for understanding human development and disease. Optimal utilization of NP cells, however, requires an enhanced ability to monitor these cells in vitro and in vivo. Here we report production of the first genetically modified self-renewing human embryonic stem cell–derived NP cells that express fluorescent proteins under constitutive as well as lineage-specific promoters, enabling tracking and monitoring of cell fate. Nucleofection, transfection, and lentiviral transduction were compared for optimal gene delivery to NP cells. Transduction was most efficient in terms of transgene expression... Neuromics news http://www.medworm.com/rss/comments.php?id=2743926 Fri, 28 Aug 2009 18:25:00 +0100 2743926 http://www.medworm.com/index.php?rid=2715770&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Fstemez-np1-neural-progenitors-now.html We are pleased to announce the introduction of our STEMEZ TMhNP1 Human Neural Progenitors Discovery Kit. The kit was developed by our partner, ArunA Biomedical. Description/Data:STEMEZTM hNP1 Human Progenitors are fully differentiated are derived as adherent cells from hESC WA09 line. The cells are shipped frozen in a vial with 1 x 106 cells; once thawed they should be immediately plated on a Matrigel (or other suitable extracellular matrix protein)-coated dish and maintained in the accompanying serum-free medium. The neurons should be used within 14 days of thawing. These cells have the capabilities to:Display immunoreactive properties consistent with neural precursors and can be maintained in a proliferative state in monolayer cultures.Differentiate under serum-free conditions into neuro... Neuromics news http://www.medworm.com/rss/comments.php?id=2715770 Thu, 20 Aug 2009 01:30:00 +0100 2715770 http://www.medworm.com/index.php?rid=2711924&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Fregenerating-axons-can-be-guided-and.html This is an interesting updateRegenerating Axons Can Be Guided And Reform Connections After Spinal Cord InjuryWe support this Spinal Cord Injury and Repair with: Axon Growth and Guidance Antibodies Neurotrophins-Neuron/Glial Marker Stem Cell Research ReagentsSTEMEZ (TM) Human Neuron Discovery KitsShared via AddThis (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2711924 Tue, 18 Aug 2009 16:47:00 +0100 2711924 http://www.medworm.com/index.php?rid=2705075&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Flink-between-inflammation-and-tau.html Providing reagents for Alzheimer's Disease Researchers is a strategic focus area for us. This includes making a variety of Primary Neuronal Cultures available to these researchers.We evolve the strategy based on Researcher input and how the cultures are being referenced in publications. This reference is especially satisfying because of the vangaurd findings:Lisette T. Arnaud, Natura Myeku and Maria E. Figueiredo-Pereira. Proteasome–caspase–cathepsin sequence leading to tau pathology induced by prostaglandin J2 in neuronal cells. Journal of Neurochemistry. Volume 110 Issue 1, Pages 328 - 342."Our data suggest a potential sequence of events triggered by the neurotoxic product of inflammation PGJ2 leading to tau pathology. The accumulation of Ub proteins is an early response. If cells fa... Neuromics news http://www.medworm.com/rss/comments.php?id=2705075 Sun, 16 Aug 2009 14:02:00 +0100 2705075 http://www.medworm.com/index.php?rid=2703792&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Fsurvival-of-efferent-synapses-on.html Dr Douglas Vetter (Tufts University School of Medicine) and team recently published a study that amplifies the understanding of the development, function and maintenance of auditory system function.Their data strongly suggest that hair cell responses induced and/or modulated by Olivocochlear (OC) activation are necessary for the survival of OC innervation and that these responses must involve SK2-mediated hyperpolarization.It also includes an excellent image of Olivocochlear fibers degeneration in SK2−/− mice. One of the makers used was our Tuj 1 (Neuron-specific class III beta-tubulin).Vidya Murthy, Stéphane F. Maison, Julián Taranda, Nadeem Haque, Chris T. Bond , A. Belén Elgoyhen, John P. Adelman, M. Charles Liberman, Douglas E. Vetter. SK2 channels are required for function and ... Neuromics news http://www.medworm.com/rss/comments.php?id=2703792 Sun, 16 Aug 2009 00:00:00 +0100 2703792 http://www.medworm.com/index.php?rid=2683758&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Fens-development-markers.html This is an excellent study on the developing Enteric Nervous Stem (ENS). This system is often referred to as the "second brain".Despite this, neurogenesis has been much less studied in the ENS than in the brain. Understanding how neurons are formed in the gut is the foundation for finding cures for ENS disorders. The key finding here is that the ability of 5-HT4 receptors to unmask a regulation of enteric neurogenesis in adult animals suggests that the mature ENS is capable of an unexpected degree of plasticity (potentially good new for discovering therapies for ENS related disorders).Min-Tsai Liu, Yung-Hui Kuan, Jingwen Wang, René Hen, and Michael D. Gershon. 5-HT4 Receptor-Mediated Neuroprotection and Neurogenesis in the Enteric Nervous System of Adult Mice. The Journal of Neuroscience,... Neuromics news http://www.medworm.com/rss/comments.php?id=2683758 Sun, 09 Aug 2009 02:45:00 +0100 2683758 http://www.medworm.com/index.php?rid=2670675&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F08%2Fspinal-cord-injury-repair.html Neuromics has featured an overview of Dr. Matt Ramer's Research on Spinal Cord Injury and "Finding Fixes for Injured Nerves" on our Neuroscience News Blog.We wanted to share a recent publication authored by Dr. Mark Tuszynski and his team of researchers at USCD. Here's the good news:"NT-3 expression in the correct target led to reinnervation of the nucleus gracilis in a dose-related fashion, whereas NT-3 expression in the reticular formation led to mistargeting of regenerating axons. Axons regenerating into the nucleus gracilis formed axodendritic synapses containing rounded vesicles, reflective of pre-injury synaptic architecture. Thus, we report for the first time, to the best of our knowledge, the reinnervation of brainstem targets after SCI and an essential role for chemotropic axon gu... Neuromics news http://www.medworm.com/rss/comments.php?id=2670675 Wed, 05 Aug 2009 01:23:00 +0100 2670675 http://www.medworm.com/index.php?rid=2657418&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Ffgf-basic-and-cell-proliferation-in.html We are pleased with the growth of our Fibroblast Growth Factor Proteins.Here's a recent publications referencing use of our FGF basic in Endothelial Cell Cultures.Harun Elmasri, Cagatay Karaaslan, Yaroslav Teper, Elisa Ghelfi, MeiQian Weng, Tan A. Ince, Harry Kozakewich, Joyce Bischoff, and Sule Cataltepe. Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells. FASEB J, Jul 2009; doi:10.1096/fj.09-134882...VEGF-A 165 (VEGF) and human basic FGF (bFGF) were purchased from R&D Systems (Minneapolis, MN, USA) and Neuromics (Edina, MN, USA), respectively...Featured ReagentFGF basic (146 aa)Related Reagents:ProteinsFGF basic (146 aa), carrier-freeFGF basic (157 aa)FGF basic (157 aa), carrier-freeFibroblast Growth FactorsAntibodies:FGF2All S... Neuromics news http://www.medworm.com/rss/comments.php?id=2657418 Thu, 30 Jul 2009 16:55:00 +0100 2657418 http://www.medworm.com/index.php?rid=2644976&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Ftrpa1-in-craniofacial-pain.html Dr. Jin Ro and his team reference use of TRP antibodies in their recent study of craniofacial muscle nociception and mechanical hyperalgesia:Jin Y. Ro, Jong-Seok Lee and Youping Zhang. Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia. doi:10.1016/j.pain.2009.04.021.... TRPA1 (1:1500; rat polyclonal, Neuromics)...Featured ReagentsTRPA1-Cat#: RA14135Related Reagents:TRPA1 for WBVR1 N-Terminus (TRPV1)VR1 C-terminus (TRPV1)VR1 C-Terminus (TRPV1) - mouse specificVR1 (TRPV1)-GoatVR like-3 (TRPV3)All TRPV (Vanilloid); TRPM; TRPA and TRPCsPain and Inflammation Antibodies (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2644976 Tue, 28 Jul 2009 00:08:00 +0100 2644976 http://www.medworm.com/index.php?rid=2641239&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Fstemez-hn2-human-neurons-data.html Neuromics rolled out STEMEZTM hN2 Human Neurons Discovery Kits several months ago.Applications for these include: cellular model studies, high content screening, developmental studies, RNAi studies and genetic manipulation.Drilling down further, I am pleased to present Electro-physiology and related data generated by Aruna and collaborators: hN2 Cells-Electro Phys Data SupplementhN2-Whole Cell Voltage ClampFigure. hN2 cells can produce inward currents that generate action potentials. (A) Isolated hN2 with significant neurite growth 1 week after plating . This cell was subjected to whole cell voltage clamp utilizing a potassium gluconate based intracellular solution. (B) Voltage gated inward and outward currents were elicited from this cell with depolarizing voltage steps. (C) Inward curren... Neuromics news http://www.medworm.com/rss/comments.php?id=2641239 Sun, 26 Jul 2009 22:37:00 +0100 2641239 http://www.medworm.com/index.php?rid=2634209&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Fstem-cell-markers.html In this study the authors demonstrate a between SSCs and RET.ETV5 is expressed beginning in fetal Sertoli cells and can potentially have effects on both neonatal Sertoli and germ cells. In addition, ETV5 has critical effects on neonatal spermatogonial proliferation, which may involve impaired signaling through the RET receptor.Gaurav Tyagi, Kay Carnes, Carla Morrow, Natalia V. Kostereva, Gail C. Ekman, Daryl D. Meling,Chris Hostetler, Michael Griswold, Kenneth M. Murphy, Rex A. Hess, Marie-Claude Hofmann and Paul S. Cooke. Loss of Etv5 Decreases Proliferation and RET Levels in neonatal Mouse Testicular Germ Cells and Causes an Abnormal First Wave of Spermatogenesis. DOI:10.1095/biolreprod.108.075200Images: Ret IHC in WT and Etv5-/- testes (n=4). The intensity of RET staining per spermatogo... Neuromics news http://www.medworm.com/rss/comments.php?id=2634209 Thu, 23 Jul 2009 13:27:00 +0100 2634209 http://www.medworm.com/index.php?rid=2621416&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Fjove-generation-of-single-cell.html Shared via AddThis (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2621416 Tue, 21 Jul 2009 11:18:00 +0100 2621416 http://www.medworm.com/index.php?rid=2616625&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F07%2Fstem-cells-science-blog.html Posted using ShareThis (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2616625 Sun, 19 Jul 2009 22:25:00 +0100 2616625 http://www.medworm.com/index.php?rid=2522412&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F06%2Fhes-1-and-develpmental-neurobiology.html Developmental Neurobiology is a core area of focus for Neuromics. Our goal is to provide a growing catalog of research proven reagents to Researchers. Their work is key to unlocking root causes of developmental related diseases and defects.We continue to see use of our reagents in publications by leading labs. Here's a new publication by Dr. Paul Trainor and his team at Stower's Institute referencing use of our HES-1 antibody:Angelo Iulianella, Madhulika Sharma, Greg B. Vanden Heuvel, and Paul A. Trainor. Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord. Development 136, 2329-2334 (2009). doi: 10.1242/10.1242/dev.032128....anti-Hes1 (Neuromics, Edina, MN, USA)...Related Reagents:Notch1Notch3Jagged1All Neuron-Glial MarkersNeurotrophins... Neuromics news http://www.medworm.com/rss/comments.php?id=2522412 Fri, 26 Jun 2009 14:50:00 +0100 2522412 http://www.medworm.com/index.php?rid=2508327&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F06%2Fpotent-stem-cell-markers.html The potency of our Stem Cell Research Reagents is confirmed by the growng list of publication referencing them.Here're the latest:Saishu Yoshida, Takako Kato, Takao Susa, Li-yi Cai, Michie Nakayama, Yukio Kato. PROP1 coexists with SOX2 and induces PIT1-commitment cells. Biochemical and Biophysical Research Communications, Volume 385, Issue 1, 17 July 2009, Pages 11-15... SOX2 (1:500 dilution, Neuromics, Edina, MN, USA)...Saravanan Karumbayaram, Bennett G. Novitchb, Michaela Patterson, Joy A. Umbach, Laura Richter, Anne Lindgren, Anne E. Conway, Amander T. Clark, Steve A. Goldman, Kathrin Plath, Martina Wiedau-pazos, Harley I. Kornblum, William E. Lowry. Directed Differentiation of Human-Induced Pluripotent Stem Cells Generates Active Motor Neurons. Stem Cells Vol. 27 No. 4 April 2009, pp. ... Neuromics news http://www.medworm.com/rss/comments.php?id=2508327 Sun, 14 Jun 2009 14:21:00 +0100 2508327 http://www.medworm.com/index.php?rid=2397746&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F05%2Fdetecting-apoptosis-in-real-time.html Kudos to Drs. Aaron Thomas and Carol Erickson for their novel use of our Magic Red™ Real Time! Apptosis Kits. Here's the related publication:Aaron J. Thomas and Carol A. Erickson. FOXD3 regulates the lineage switch between neural crest-derived glial cells and pigment cells by repressing MITF through a non-canonical mechanism. Development, Apr 2009; doi:10.1242/dev.031989....Apoptosis was assayed using Magic Red Caspases 3&7 reagent (Neuromics)...Related Reagents:FLIVO™ Polycaspase Live!, in vivo Apoptosis Kits-NewFLICA™ in vitro Caspase KitsFast!-Use Caspase kits to quantitate apoptosis via active caspases in whole, living cells. These kits do not use ELISA or any antibodies for detectionFLISP™ Serine Protease Detection KitsMeasure chymotrypsin-like proteaseactivation in whole ... Neuromics news http://www.medworm.com/rss/comments.php?id=2397746 Thu, 07 May 2009 12:30:00 +0100 2397746 http://www.medworm.com/index.php?rid=2300740&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F04%2Frage-and-pneumonia.html We are working with customers and collaborators to strengthen our product offerings for Immune Response Researchers.An interesting finding on Receptor for Advanced Glycation End Products (RAGE) and response to S. pneumoniae pneumonia infection just crossed our radar. Dr. Marieke A. D. van Zoelen and team published evidence that RAGE plays a detrimental role in the host response to S. pneumoniae pneumonia by facilitating the bacterial growth and dissemination and concurrently enhancing the pulmonary inflammatory and procoagulant response. Data include use of our RAGE-Cat#: GT15030.Here's the publication and related data:Marieke A. D. van Zoelen, Marcel Schouten, Alex F. de Vos, Sandrine Florquin, Joost C. M. Meijers, Peter P. Nawroth, Angelika Bierhaus, and Tom van der Poll. The Receptor fo... Neuromics news http://www.medworm.com/rss/comments.php?id=2300740 Thu, 02 Apr 2009 19:51:00 +0100 2300740 http://www.medworm.com/index.php?rid=2300741&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F03%2Fintroducing-human-neuron-kits.html hN2 Human Neurons Discovery Kit-NewEnergize you Research!Neuromics has formed an alliance with Aruna Biomedical. This Alliance gives us the capabilities to bring you the reliable, robust and highly scalable hN2TMHuman Neurons Discovery Kits.These kits are designed to reduce basic Neuroscience Research and Drug Discovery timelines. Potential applications include: cellular model studies, high content screening, developmental studies, RNAi studies and genetic manipulation.Approximate Yield=1,000,000 healthy Neurons.hN2 Human Neurons Discovery Kit Details (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=2300741 Mon, 30 Mar 2009 22:54:00 +0100 2300741 http://www.medworm.com/index.php?rid=2261074&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F03%2Famyloid-beta-and-e18-primary.html We would like to feature an interesting application of our E18 Rat Primary Hippocampal Neurons.Related Publication:Karunya K. Kandimalla1, Olenych G. Scott, Smita Fulzele1, Michael W. Davidson, Joseph F. Poduslo. Mechanism of Neuronal versus Endothelial Cell Uptake of Alzheimer's Disease Amyloid β Protein. PLoS ONE 4(2): e4627. doi:10.1371/journal.pone.0004627.... Rat primary hippocampal (RPH) neurons were isolated from the hippocampii of 18-day-old embryonic Sprague Dawley rat brains (Neuromics, Edina, MN). The hippocampii were dispersed using a fire polished Pasteur pipette and plated on poly-D-lysine (Sigma-Aldrich, St. Louis, MO) coated glass cover slips in B-27 neurobasal medium containing 0.5 mM glutamine and 25 µM glutamate (Invitrogen, Carlsbad, CA). The neuronal cells were grown... Neuromics news http://www.medworm.com/rss/comments.php?id=2261074 Sat, 14 Mar 2009 01:15:00 +0100 2261074 http://www.medworm.com/index.php?rid=2223976&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F02%2Ftrpv1-with-twist.html Kudos to Dr. Marna Erickson and her team at the University of Minnesota. They recently published excellent work demonstrating a relationship between TRPV1 -EGFR signaling and skin cancer.Ann M. Bode, Yong-Yeon Cho, Duo Zheng, Feng Zhu, Marna E. Ericson, Wei-Ya Ma, Ke Yao, and Zigang Dong. Transient Receptor Potential Type Vanilloid 1 Suppresses Skin Carcinogenesis.Cancer Res., Feb 2009; 69: 905 - 913."TRPV1 interacts with EGFR, leading to EGFR degradation. Notably, the absence of TRPV1 in mice results in a striking increase in skin carcinogenesis. The TRPV1 is the first membrane receptor shown to have a tumor-suppressing effect associated with the down-regulation of another membrane receptor."...dorsal skin samples (100 mum) were processed and immunostained. For the human skin cancer tissu... Neuromics news http://www.medworm.com/rss/comments.php?id=2223976 Sat, 28 Feb 2009 16:31:00 +0100 2223976 http://www.medworm.com/index.php?rid=2207088&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F02%2Ftrpv1-and-retinal-ganglion-cell.html The publications referencing our TRPV (Vanilloid); TRPM; TRPA and TRPC Antibodies keep on coming.In this February 2009 publication, Dr. David Calkins and his team at Vanderbilt demontrate that Retinal Ganglian Cells express the TRPV1 channel and that TRPV1 activation contributes to their death with exposure to hydrostatic pressure . We also demonstrated that activation of TRPV1 alone was sufficient to induce apoptosis of RGCs.Rebecca M. Sappington, Tatiana Sidorova, Daniel J. Long, and David J. Calkins. TRPV1: Contribution to Retinal Ganglion Cell Apoptosis and Increased Intracellular Ca2+ with Exposure to Hydrostatic Pressure. Invest. Ophthalmol. Vis. Sci., Feb 2009; 50: 717 - 728....we used rabbit anti-mouse TRPV1 IgG (1:500; catalog number RA14113; Neuromics, Edina, MN) against the abso... Neuromics news http://www.medworm.com/rss/comments.php?id=2207088 Sun, 22 Feb 2009 23:11:00 +0100 2207088 http://www.medworm.com/index.php?rid=2201923&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F02%2Fneurotrophin-expression-in.html Dr. Y.K. Prakash and his team at Mayo Clinic College of Medicine have published a study of Neurotrophin Signaling in Airway Smooth Muscle (ASM) cells. Here they demonstrate that BDNF/TrkB signaling (but not BDNF/p75NTR) in human ASM cells regulates [Ca2+]i responses to agonist in both normal ASM, as well as with inflammation induced by TNFα. These effects suggest an important role of NT signaling in inflammation-induced changes in ASM contractility.Y.S. Prakash, Michael A Thompson, and Christina M Pabelick. Brain Derived Neurotrophic Factor in TNF Modulation of Ca2+ in Human Airway Smooth Muscle. Am. J. Respir. Cell Mol. Biol., Feb 2009; doi:10.1165/rcmb.2008-0151OC.... goat polyclonal anti-TrkB-Cat#: GT15080 (Neuromics, Minneapolis, MN; 1:1000 dilution)...Featured ReagentsTrkB-Cat#: GT15... Neuromics news http://www.medworm.com/rss/comments.php?id=2201923 Fri, 20 Feb 2009 00:55:00 +0100 2201923 http://www.medworm.com/index.php?rid=2184341&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F02%2Fsubstance-p-pub.html We re-introduced our Substance P Whole Serum Guinea Pig and Substance P Purified-Guinea Pig Antibodies in 2008.The feedback has been positive. Related to this, here's a recent reference:Maureen S. Riedl, Stephen A. Schnell, Aaron C. Overland, Anne-Julie Chabot-Doré, Anna M. Taylor, Alfredo Ribeiro-Da-Silva, Robert P. Elde, George L. Wilcox, Laura S. Stone. Coexpression of 2A-adrenergic and -opioid receptors in substance P-containing terminals in rat dorsal horn. The Journal of Comparative Neurology Volume 513 Issue 4, Pages 385 - 398 Published Online: 29 Jan 2009 Copyright © 2009 Wiley-Liss, Inc., A Wiley Company....guinea pig anti-SP (1:500; Neuromics Antibodies)...Related Reagents:Substance P-RabbitSubstance P-MouseNeurokinin-1 (NK 1) (RA25001)Neurokinin-1 (NK 1) Human (RA25003)Neuroki... Neuromics news http://www.medworm.com/rss/comments.php?id=2184341 Thu, 12 Feb 2009 14:22:00 +0100 2184341 http://www.medworm.com/index.php?rid=2129771&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F01%2Fopioid-receptor-antibodies-that-work.html We have Opioid Receptor Antibodies that work hard for our customers.They are extensively referenced. We feature select publications and are pleased to feature the latest referencing staining of mouse neurons using our Kappa Opioid Receptor....Dominika Labuz, Yvonne Schmidt, Anja Schreiter, Heike L. Rittner, Shaaban A. Mousa and Halina Machelska. Immune cell–derived opioids protect against neuropathic pain in mice. J. Clin. Invest. doi:10.1172/JCI36246. Copyright © 2009, The American Society for Clinical Investigation......κ-opioid receptor (1:800, Neuromics)...All Opioid Receptor Publications-Mu, Kappa and DeltaPublished Reagents:Mu Opioid Receptor-RabbitMu Opioid Receptor-Guinea PigDelta Opioid Receptor 358-372Kappa Opioid ReceptorRelated Reagents:Opioid ReceptorsAll Pain and Inflamma... Neuromics news http://www.medworm.com/rss/comments.php?id=2129771 Fri, 23 Jan 2009 19:25:00 +0100 2129771 http://www.medworm.com/index.php?rid=2105137&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F01%2F24-ohc-27-ohc-and-alzheimers.html Dr. Othman Ghribi and his team at University of North Dakota have just published interesting results regarding the relationship between 24-hydroxycholesterol (24-OHC) and 27-hydroxycholesterol (27-OHC).27-OHC increased levels of amyloid precursor protein (APP) as well as beta-secretase (BACE1)the enzyme that cleaves APP to yield Abeta, a molecule that causes alzheimer's.24-OHC are associated with increased levels of sAPPalpha, suggesting that 24-OHC favors the processing of APP to the non-amyloidogenic pathway.Futher study of this relationship between 27 and 24-OHC could yield possible targets for decreasing the cleavage of alzheimer's causing proteins and result in more step towards the Azheimer's therapies.Here's the publication.Prasanthi JRP, Huls A, Thomasson S, Thompson A, Schommer E,... Neuromics news http://www.medworm.com/rss/comments.php?id=2105137 Wed, 14 Jan 2009 15:39:00 +0100 2105137 http://www.medworm.com/index.php?rid=2098442&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2009%2F01%2Fdecember-2008-featured-pubs.html Intrathecal Delivery of siRNAAnother publication referencing successful delivery of siRNA using i-FectSuneeta Tumati, Tally Largent Milnes, Henry I. Yamamura, Todd W. Vanderah, William R. Roeske and Eva V. Varga. Intrathecal Raf-1-selective siRNA attenuates sustained morphine-mediated thermal hyperalgesia. doi:10.1016/j.ejphar.2008.10.033...siRNAs stock solutions (100 ?M) were prepared in double distilled RNAse free water and stored in aliquots at ?80 °C. For intrathecal treatment, aliquots of the stock solution (2 ?g of the appropriate siRNA) were mixed (1:5 v/v)with i-Fect transfection reagent (Neuromics, Edina, MN). After recovery from the surgery (5-7 days), the animals received intrathecal injections (2 ug! siRNA/1 0 ul/rat) of either a lipid encapsulated Raf-1-selective siRNA mixtur... Neuromics news http://www.medworm.com/rss/comments.php?id=2098442 Mon, 12 Jan 2009 22:37:00 +0100 2098442 http://www.medworm.com/index.php?rid=1981827&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F11%2Fexcellent-trpv1-n-ihc-and-wb.html Kudos to Dr. Federica MF van Dissel-Emiliani and her team for the excellent Immunohistochemistry and Western Blot results using our TRPV1-N Antibody(Catalog #: RA10110) . The antibody was in their study demonstrating the sensitivity of spermatogenesis to capsaicin.Here's the related publication:Sefika C Mizrak, Bart M Gadella, Hatice Erdost, Aytekin Ozer, Ana MM van Pelt, Federica MF van Dissel-Emiliani. Spermatogonial stem cell sensitivity to capsaicin: An in vitro study. Reproductive Biology and Endocrinology 2008, 6:52 doi:10.1186/1477-7827-6-52.Anti TRPV1 antibody staining: Bouin's fixed, paraffin embedded 5 um-thick rat testis sections were deparaffinized and boiled in a microwave oven (700 Watt) 3x10 min in sodium citrate buffer (0.1 mM, pH=6) for antigen retrieval. All subsequent in... Neuromics news http://www.medworm.com/rss/comments.php?id=1981827 Sat, 22 Nov 2008 22:18:00 +0100 1981827 http://www.medworm.com/index.php?rid=1956016&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F11%2Fdrg-neurons-now-available.html Primary Rat DRGs are live neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration.Please note: It is important to review Protocol/Datasheet prior to ordering. There is a unique step for making the dissociation enzyme solution. Do not hesitate to call or e-mail me (612-801-1007 or pshuster@neuromics.com) should you have questions.Image: DRGs cultured on Calf Skin Collagen.References:-A dissection and Tissue Culture Manual of the Nervous System (1989). A. Shahar, J.D. Vellis, A. Vernadakis, B. Haber (Eds.), Dissociated Spinal Cord - Dorsal Root Ganglion Cultures on Plastic Tissue Culture Dishes and Glass... Neuromics news http://www.medworm.com/rss/comments.php?id=1956016 Thu, 13 Nov 2008 00:59:00 +0100 1956016 http://www.medworm.com/index.php?rid=1930943&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F11%2Fpower-trio-of-pain-research-antibodies.html I received a call from a customer asking if there were any publications referencing our rabbit Alpha 2a Adrenergic Receptor antibody. The resulting search found an article referencing excellent results using a trio of of our pain research antibodies. The research was conducted by our friend, Dr. Hui-Lin Pan and his team at University of Texas M.D. Anderson Cancer Center.Shao-Rui Chen, Hao-Min Pan, Timothy E. Richardson, and Hui-Lin Pan. Potentiation of Spinal α2-Adrenoceptor Analgesia in Rats Deficient in TRPV1- Expressing Afferent Neurons. Published online 2007 March 24. doi: 10.1016/j.neuropharm.2007.03.009.Images: Double immunofluorescence labeling showing α2C-AR- and TRPV1-immmunoreactivity in the spinal cord dorsal horn of one vehicle-treated and one RTX-treated rat. Representative ... Neuromics news http://www.medworm.com/rss/comments.php?id=1930943 Tue, 04 Nov 2008 01:22:00 +0100 1930943 http://www.medworm.com/index.php?rid=1884100&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F10%2Fpurinergic-receptor-pubs.html Our Purinergic Receptor Antibodies have recently been referenced in several publications.In the first study 5 of our antibodies are used. It investigates the expression of purinergic P2 receptors in oxygen-induced retinal neovascularization.Sylvia Sarman; Jorge Mancini; Ingeborg van der Ploeg; J. Oscar Croxatto; Anders Kvanta; Juan E. Gallo. Involvement of Purinergic P2 Receptors in Experimental Retinal Neovascularization. Current Eye Research. 10.1080/02713680701885470 ....Five eyes (5 animals) from either hyperoxa- or non-hyperoxia-treated mice were enucleated and fixed for 48 hr at 4°C in paraformaldehyde (Sigma-Aldrich, St Louis, MO, USA). Afterward, eyes were immersed for cryoprotection in graded sucrose solution (5% overnight, 10%, 15%, and 20%) and interlocked with resin. Fourteen ... Neuromics news http://www.medworm.com/rss/comments.php?id=1884100 Thu, 16 Oct 2008 16:08:00 +0100 1884100 http://www.medworm.com/index.php?rid=1866174&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F10%2Fneuronneuron-glial-markers.html We have a comprehensive and growing catalog of Neuron/Glial Marker Antibodies and Proteins.These reagents must work everytime in our customers' applications. Quality is confirmed by pro-active customers follow up and publication referencing the reagents.Here we have several recent publications.We are pleased to first feature Dr. Dr. Juana Maria Pasquini, University of and colleagues from University of Buenos Aires. She and her team use our Olig1,2,3 as a marker to study de-myelinating disease.P.G. Franco, L. Silvestroff, E.F. Soto and J.M. Pasquini. Thyroid hormones promote differentiation of oligodendrocyte progenitor cells and improve remyelination after cuprizone-induced demyelination. doi:10.1016/j.expneurol.2008.04.039...Olig 1-2-3 antibodies were from Neuromics Antibodies (Edina, MN)... Neuromics news http://www.medworm.com/rss/comments.php?id=1866174 Thu, 09 Oct 2008 17:59:00 +0100 1866174 http://www.medworm.com/index.php?rid=1851624&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F10%2Fsirna-mediated-gene-silencing.html Dr. Josephine Lai (Professor of Pharmacology, University of Arizona) is pioneer in developing experimental designs and methods for delivering siRNA to the CNS for gene expression analysis.She and her team have documented these in the publication:Modulating Sensory Systems Using RNAi(pdf - 187Kb)© 2007 LaiFor researchers desiring to effectively deliver siRNA to the CNS for gene expression of analysis of specific receptors, this publication offers proven methods. These include:The Choice of siRNAChoosing and Optimizing Transfection Reagents for siRNA Delivery to the Nervous SystemDelivery Systems-Microinjection and Infusion (using mini-osmotic pumps)ValidationWe will continue to track advances by Dr. Lai and team (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1851624 Sat, 04 Oct 2008 13:39:00 +0100 1851624 http://www.medworm.com/index.php?rid=1841695&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F10%2Fskeletal-muscle-derived-stem-cell.html Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Masahiro Nitta, Akio Hoshi, Akira Akatsuka. Skeletal Muscle–Derived CD34+/45- and CD34-/45- Stem Cells Are Situated Hierarchically Upstream of Pax7+. Cells. Stem Cells and Development. August 1, 2008, 17(4): 653-668. doi:10.1089/scd.2008.0070....anti-nucleostemin (1:200; overnight; Neuromics, Edina, USA) for proliferative capacity...Featured Reagent:Nucleostemin-Cat#:15050Related Reagents:Stem Cell ReagentsNeuron/Glial Markers (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1841695 Wed, 01 Oct 2008 15:21:00 +0100 1841695 http://www.medworm.com/index.php?rid=1816428&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Fhuman-embryonic-stem-cell.html Here's a protocol that could be of interest to hESC researchers:Elizabeth S Ng, Richard Davis, Edouard G Stanley & Andrew G Elefanty. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies. Nature Protocols 3, - 768 - 776 (2008). (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1816428 Tue, 23 Sep 2008 17:42:00 +0100 1816428 http://www.medworm.com/index.php?rid=1810225&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Fpdgfs-in-non-small-cell-lung-cancer.html Serving Cancer and Tumorigenesis Researhers is a central component to our business strategy. This means constantly building our catalog of Cancer Antibodies and Cancer Proteins.I personally follow up with every customer that purchases our Cancer Research Reagents. This ensures they work as expected. In most cases, this is validate. If not, we fix the expressed issues.We are now seeing more confirmation by the reagents being referenced in publications. In this publication, our PDGF-C antibody was used as a marker for Non-small Cell Lung Cancer Tumors."In univariate analyses, high tumor cell expression of PDGF-B (p 0.001), PDGF-C (p 0.01), and PDGFR- (p 0.026) were negative prognostic indicators for disease-specific survival."Donnem, Tom MD, Al-Saad, Samer MD, Al-Shibli, Khalid MD, Andersen,... Neuromics news http://www.medworm.com/rss/comments.php?id=1810225 Fri, 19 Sep 2008 15:45:00 +0100 1810225 http://www.medworm.com/index.php?rid=1806933&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Frat-sensory-neurons-and-nk-1.html A publication featuring our Neurokinin-1 (NK 1) Receptor just crossed my radar. It is authored by our friend, Dr. Matt Ramer.Matt and his team at University of British Columbia study primary sensory nerve cells (neurons), which are responsible for the transmission of somatic (bodily) sensations such as touch, pain, hot, cold and so on from the periphery (skin, muscles and viscera) to the central nervous system (CNS, spinal cord and brain). His research extends to therapeutic potential of neurotrophins on regeneration in spinal cord injury and deafferentation pain.In the past several years, he has generouly shared NT-3 and BDNF IHC data with us.Here's a link to the publication:Matt Ramer. Anatomical and functional characterization of neuropil in the gracile fasciculus. The Journal of Compar... Neuromics news http://www.medworm.com/rss/comments.php?id=1806933 Thu, 18 Sep 2008 20:36:00 +0100 1806933 http://www.medworm.com/index.php?rid=1790765&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Felisa-techniques-and-protocols.html Power Point of ELISA Methods and Protocols(pps - 1.4M)This teaching guide covers the three major types of ELISA: indirect, competitive, and sandwich. It integrates theory with practice, to help you understand what you are doing, and help you to do it!Courtesy of our friends at Novus Biologicals. (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1790765 Sat, 13 Sep 2008 17:52:00 +0100 1790765 http://www.medworm.com/index.php?rid=1786517&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Fischemia-inflammatory-response-and.html We would like to send Kudos to Dr. Yan Xu and his colleagues at University of Pittburgh for their findings on inflammatory response in Golbal Ischemia. Their work was recently published:Aaron Hirko, Renee Dallasen, Sachiko Jomura, Yan Xu. Modulation of Inflammatory Responses after Global Ischemia by Transplanted Umbilical-Cord Matrix Stem Cells. Stem Cells First published online August 21, 2008; doi:doi:10.1634/stemcells.2008-0075Secondary to Cardiac Arrest is Brain Damage do to lack of blood flow. This is marked by a delayed loss of Neurons in CA1 hippocampus region of the brain due to inflammatory response.The story timeline of this response is good then bad with interesting twists. The delay in neuronal loss is linked to initial inflammation. It involves both reactive astrocytes (astroc... Neuromics news http://www.medworm.com/rss/comments.php?id=1786517 Thu, 11 Sep 2008 18:09:00 +0100 1786517 http://www.medworm.com/index.php?rid=1776232&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F09%2Fneurotoxicity-testing.html We combine our expertise in providing fresh and healthy:E18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURESE18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURESE18 Mouse Neuronal Tissue -NEURON CULTURESE18 Mouse Neuronal Tissue -ASTROCYTE CULTURESAND Apoptosis Research Reagentsto help researchers more effectively study Neurotoxicity.Images: Polycaspase Assay Kit, green was used to assess cell death in primay rat hippocampal neurons.Cells were plated on 25-mm poly-l-lysine-coated coverslips at 300,000 cells per coverslip. Cells were used at 4 or 8 days in vitro. Composite imagae (A) 3 out of 4 cells are apoptotic (green). No cells were necrotic as both of the PI-positive cells were FLICA-positive; they had compromised membranes and were probably in the late stages of apoptosis rather th... Neuromics news http://www.medworm.com/rss/comments.php?id=1776232 Mon, 08 Sep 2008 15:17:00 +0100 1776232 http://www.medworm.com/index.php?rid=1740083&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F08%2Fno-pain-no-gain.html We work hard to make sure our Pain and Inflammation antibodies continue to be a gold standard for researchers. We follow up with virtually all researchers to make sure they work to expecations in each unique application.We also look for references in current publication. Although published in 2006, this one just crossed our radar scope. It contains multiple images of 3 of our top sellers: Mu Opioid Receptor, VR1 C-terminus (TRPV1) and VR1 N-Terminus (TRPV1).Shao-Rui Chen and Hui-Lin Pan. Loss of TRPV1-expressing Sensory Neurons Reduces Spinal : Opioid Receptors but Paradoxically Potentiates Opioid Analgesia. J Neurophysiol (February 8, 2006). doi:10.1152/jn.01343.2005 (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1740083 Thu, 28 Aug 2008 18:40:00 +0100 1740083 http://www.medworm.com/index.php?rid=1714738&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F08%2Ftransthyretin-app-peptides-and.html Demographics point to increasing rates of Alhzheimer's Disease. This disease steals away the golden years of sufferers. It also costs society billions healthcare.Research for the cure marches on. Researchers know the disease is characterizedby the deposition of amyloid β-peptide (A-Beta) in the brain. The challenge is finding a way to protect the brain from these depositions or reverse the process.Dr. Isabel Cardoso and her team at Instituto de Biologia Molecular e Celular have recently published compelling work in this area. Here they provide more important evidence for the role of a Transthyretin (TTR) protective mechanism. This mechanism could include the removal of deposited A-Beta.Rita Costa, Frederico Ferreira-da-Silva, Maria J. Saraiva, and Isabel Cardos. Transthyretin Protects aga... Neuromics news http://www.medworm.com/rss/comments.php?id=1714738 Mon, 18 Aug 2008 15:28:00 +0100 1714738 http://www.medworm.com/index.php?rid=1712015&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F08%2Fmmp-9-e-coli-and-breast-cancer.html In this study, Escherichia coli K-12 colonization on the tumour microenvironment by immunohistochemistry and fluorescence microscopy in the murine 4T1 breast carcinoma model. MMP-9 and TNF-alpha expression is altered in the sites of tumors.Interestingly, the authors observed a postive change in the expression of these proteins after colonization.Stephanie Weibel, Jochen Stritzker, Matthias Eck, Werner Goebel, and Aladar A. Szalay. Colonization of experimental murine breast tumours by Escherichia coli K-12 significantly alters the tumour microenvironment. 10.1111/j.1462-5822.2008.01122.x. Cellular Microbiology. © 2008 Blackwell Publishing Ltd...goat anti-mouse MMP-9 antibody (Neuromics, Edina, MN)... (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1712015 Mon, 18 Aug 2008 14:17:00 +0100 1712015 http://www.medworm.com/index.php?rid=1710444&cid=s_37785_168_f&fid=37785&url=http%3A%2F%2Fneuromics.blogspot.com%2F2008%2F08%2Fparkinsons-disease-research-blog.html (Source: Neuromics) Neuromics news http://www.medworm.com/rss/comments.php?id=1710444 Fri, 15 Aug 2008 01:23:00 +0100 1710444