MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Nippon Ishinkin Gakk...' source. Sun, 21 Mar 2010 14:52:37 +0100 http://www.medworm.com/index.php?rid=3312419&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185865%26dopt%3DAbstract Authors: Yamaguchi M Morphology and dynamics of the spindle pole bodies (S PBs) in Exophiala dermatitidis and Cryptococcus neoformans were examined by freeze-substitution and serial ultrathin sectioning electron microscopy.The SPBs showed double forms and were located on the nuclear envelope in G1 phase, entered the nuclear membrane or the periphery of nucleus in M phase,and appeared to duplicate in early G1 phase in these yeasts.Thus, the SPBs in these yeasts are clearly different from those of Saccharomyces cerevisiae. PMID: 20185865 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312419 Sat, 27 Feb 2010 11:52:03 +0100 3312419 http://www.medworm.com/index.php?rid=3312418&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185866%26dopt%3DAbstract Authors: Maruyama N, Abe Y, Hisajima T, Hayama K, Abe S To evaluate the anti-inflammatory activity of the thiocarbamate antifungal agent liranaftate, the edema and the neutrophil accumulation detected by the activity of neutrophil marker enzyme, myeloperoxidase (MPO) ,were examined following application of liranaftate to mouse ears with inflammation induced by phorbol 12-myristate13-acetate (PMA) .Topical 20 mul administration of liranaftate in a dose-range between1-4% suppressed the increase in ear thickness 6 hr after PMA application dose-dependently. Similarly, it decreased the weight increase of an ear section after 24 hr dose-dependently. More than 1% of liranaftate also suppressed augmentation of MPO activity of the ear section. This and histological observation indicate that lir... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312418 Sat, 27 Feb 2010 11:52:03 +0100 3312418 http://www.medworm.com/index.php?rid=3312417&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185867%26dopt%3DAbstract We examined the effects of spices and herbs on Candida albicans growth using in vitro assay and therapeutic activity of some selected herbal preparations against murine oral candidiasis. All tested samples: lemongrass ( Cymbopogon citratus ) ,lemon balm ( Melissa officinalis ) ,thyme ( Thymus vulgaris ) ,rosemary ( Rosmarinus officinalis) ,roselle ( Hibiscus sabdariffa ) ,green tea ( Camellia sinensis ) ,and cassia ( Cinnamomum cassia ) inhibited Candida mycelial growth in vitro.The results of this assay showed that the anti- Candida activity of lemongrass, green tea, and cassia is stronger than that of the other tested herbs. Oral administration of lemongrass or green tea did not result in significant improvement in the murine oral candidiasis, while the administration of cassia improved ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312417 Sat, 27 Feb 2010 11:52:03 +0100 3312417 http://www.medworm.com/index.php?rid=3312416&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185868%26dopt%3DAbstract Conclusions: The clinico-epidemiological data collected can serve as reference for future research and may be useful in the development of preventive and educational strategies. PMID: 20185868 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312416 Sat, 27 Feb 2010 11:52:03 +0100 3312416 http://www.medworm.com/index.php?rid=3312415&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185869%26dopt%3DAbstract Authors: Nakayama H, Shibuya K, Kimura M, Ueda M, Iwabuchi S In recent years, incidence of invasive fungal infection has been increasing, mostly due to advances in + medicine that may produce immunocompromised individuals. Candidial infection in the central nervous system (CNS) is one of the most serious forms of blood stream infection of Candida sp. and mortality is known to be more than 50%.In this research, we employed 27 autopsies with confirmed in vasive CNS yeast infection which were confirmed. In addition to detailed morphological analysis of yeast cells in lesions, in situ hybridization was carried out with an originally designed Candida -specific peptide nucleic acid (PNA) probe to identify the candidial infection of each patient. This was followed by histopathological investi... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312415 Sat, 27 Feb 2010 11:52:03 +0100 3312415 http://www.medworm.com/index.php?rid=3312414&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20185870%26dopt%3DAbstract Authors: Hiruma M PMID: 20185870 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3312414 Sat, 27 Feb 2010 11:52:03 +0100 3312414 http://www.medworm.com/index.php?rid=3037263&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942788%26dopt%3DAbstract Authors: Mochizuki T The Japanese Dermatological Association produced some guidelines for the management of cutaneous fungal infection in cooperation with the Japanese Society for Medical Mycology, in which the importance of an accurate diagnosis of the fungal infection before antifungal treatment is emphasized. Here I comment on conventional mycological tests including direct microscopic examination and fungal cultures, which have been listed in the guidelines. Sampling of the clinical specimen is the most important step in mycological tests, so dermatologists should be aware of how and where good specimens are obtained. Direct microscopic examination of a KOH (potassium hydroxide) mounted preparation is the most simple and important test for diagnosing superficial fungal infection an... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037263 Mon, 30 Nov 2009 04:02:03 +0100 3037263 http://www.medworm.com/index.php?rid=3037262&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942789%26dopt%3DAbstract We present our classification draft based on these following descriptions. In Japan, any dermatophytosis other than favus or tinea imbricata is considered to be tinea, while outside Japan, favus and tinea imbricata are also classified as tinea. Tinea capitis is classified together with trichophytia superficialis capillitii and kerion celsi, in a group that tends to include asymptomatic carriers. Most textbooks generally classify trichophytia profunda of the glabrous skin and granuloma trichophyticum as subtypes of tinea corporis. Tinea faciei can easily be misdiagnosed, but in many cases can be distinguished from tinea corporis by its specific clinical picture. Tinea unguium is regarded as one type of onychomycosis.We present a summary of dermatomycosis treatment as a proposal for future r... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037262 Mon, 30 Nov 2009 04:02:03 +0100 3037262 http://www.medworm.com/index.php?rid=3037261&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942790%26dopt%3DAbstract Authors: Katoh T This document summarizes current knowledge about diagnosis and treatment of candidiasis affecting the skin and oral mucosa. Several clinical forms of mucocutaneous candidiasis are distinguished depending on a patient's age and infected site, e.g. Candida intertrigo, erythema mycoticum infantile, erosio interdigitalis blastomycetica, candidal paronychia and onychia, Candida onychomycosis, and oral candidiasis. The diagnosis of candidiasis is confirmed by observation of mycelial forms on microscopic examination. Since Candida yeasts (especially C. albicans) are normal inhabitants of the skin and oral mucosa, it must always be noted that positive culture does not always indicate the presence of candidal infection. The pathogenicity of Candida species is relatively low, an... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037261 Mon, 30 Nov 2009 04:02:03 +0100 3037261 http://www.medworm.com/index.php?rid=3037260&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942791%26dopt%3DAbstract Authors: Kusuhara M Sporotrichosis is a chronic infectious granuloma of skin. The detection of fungal elements in pathological examination and the isolation of Sporothrix schenckii from the lesion are requisite for diagnosis. The sporotrichin test is useful as an auxiliary examination, but a false-negative reaction might occur in some cases. Oral potassium iodide is first choice of treatment, because of its modest cost and usefulness, although gastrointestinal disorder is a frequent side effect. Itraconazole should be the second selection, and then terbinafine. Local thermotherapy is also effective as an additional therapy. Dematiaceous fungal skin infections are divided into two groups by their parasitic form, chromoblastomycosis and phaeohyphomycosis. Chromoblastomycosis is also call... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037260 Mon, 30 Nov 2009 04:02:03 +0100 3037260 http://www.medworm.com/index.php?rid=3037259&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942792%26dopt%3DAbstract Authors: Wada Y In recent years, there have been reports on increases in non-albicans, and in this study, based on non-albicans isolated in the fields of surgery and emergency care as well as departments of hematology conducting the preventive administration of antifungal agents, we investigated the detected bacterial strains, detection rates, and trends in the results for susceptibility to antifungal agents, while focusing on rare Candida spp. According to the results, in the departments of hematology, rare Candida spp. were detected at high rates and the susceptibility was low. IN comparison to the fields of surgery and emergency care, the departments of hematology featured shifts toward rare Candida spp. rather than shifts toward C. glabrata or C. krusei. In the future, it will be n... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037259 Mon, 30 Nov 2009 04:02:03 +0100 3037259 http://www.medworm.com/index.php?rid=3037258&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942793%26dopt%3DAbstract Authors: Myoken Y A proportional increase in candidemia due to non-albicans Candida species has been reported worldwide. In our hospital, 36 of 58 candidemia cases were caused by non-albicans Candida species between 1996 and 2007. Candidemia due to non-albicans Candida species is associated with fluconazole(FLCZ)exposure. In our cases, 36 of 36 non-albicans candidemia cases received FLCZ while 18 of 22 albicans candidemia cases received this drug. In general, non-albicans Candida species including C. tropicalis, C. parapsilosis, and C. guilliermondii are susceptible to FLCZ. On the other hand, C. glabrata and C. krusei exhibit decreased susceptibility to FLCZ. Our in vitro susceptibility test revealed the same results as above although C. guilliermondii showed an elevated MIC to FLCZ(4... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037258 Mon, 30 Nov 2009 04:02:03 +0100 3037258 http://www.medworm.com/index.php?rid=3037257&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942794%26dopt%3DAbstract Authors: Lee EJ, Chung JW, Choi S, Kim YS, Woo JH Mucor is a mold which exists in nature, but mucor infections of humans, even in immunocompromised hosts, are rare. Clinical manifestations of mucormycosis are nonspecific and diagnosis is based on microscopic examination and culture of biopsy specimens. Serologic test or molecular methods of speciation are used only as research tools.We investigated medical records especially for underlying diseases, clinical findings, treatment, and prognosis of patients diagnosed with rhinocerebral mucormycosis retrospectively in the Asan Medical Center.The underlying diseases were diabetes mellitus in 8 patients, acute leukemia in 2, kidney transplantation in 2, and myelodysplastic syndrome in 1 of the total 13 patients. Six patients complained of na... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037257 Mon, 30 Nov 2009 04:02:03 +0100 3037257 http://www.medworm.com/index.php?rid=3037256&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942795%26dopt%3DAbstract Authors: Abe M, Ogawa Z, Tanuma H, Kume H We performed a comparative study of the effects of centrifugation, large amounts of inoculum and incubation temperature with regard to recovery of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus from fungal suspensions in order to identify optimal processing methods for mycological examination of clinical specimens. The number of fungal colonies, except for Candida spp., isolated from respiratory specimens, and the duration of incubation needed to isolate pathogenic fungi from clinical specimens were also analyzed retrospectively.There was a difference in the number of recovered colonies, with or without centrifugation, between inoculum sizes of 10 mu l and 50 mu l, but no differences were observed in the results obtained un... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037256 Mon, 30 Nov 2009 04:02:03 +0100 3037256 http://www.medworm.com/index.php?rid=3037255&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942796%26dopt%3DAbstract Authors: Inouye S, Takahashi M, Abe S The antifungal activity of 43 hydrosols, 7 herbal teas and 12 essential oils was determined using Candida albicans as a test organism. All of the hydrosols examined showed more potent inhibition against the filamentous form than the yeast form of C. albicans. In particular, the filamentous form was markedly inhibited by seven hydrosols, of which monarda, santolina and clove water also inhibited the growth of the yeast form. Most of the inhibitory activity of the hydrosols was correlated with that of their respective major components. Poor correlation was observed between the inhibition of filament formation and the growth inhibition of the yeast form among the hydrosols examined, among essential oils and among the major components of hydrosols and ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037255 Mon, 30 Nov 2009 04:02:03 +0100 3037255 http://www.medworm.com/index.php?rid=3037254&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942797%26dopt%3DAbstract We report two cases of candidal onychomycosis with severe nail deformities. Case 1: The patient was an 81-year-old man who complained of onycholysis and nail deformity of the right forefinger nail which had occurred over a period of a year. He had no obvious previous illness. Case 2: The patient was an 81-year-old woman who complained of nail deformity with periungual erythema which had occurred over a period of several months. She had been treated with oral corticosteroid for bronchial asthma and with Ca blocker for hypertension for a long period.The initial KOH-prepared direct microscopy in each case failed to detect any spores or pseudohyphae. Therefore, an incisional biopsy was performed in both cases. Histopathological findings demonstrated numerous fungal elements with similar appear... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037254 Mon, 30 Nov 2009 04:02:03 +0100 3037254 http://www.medworm.com/index.php?rid=3037253&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942798%26dopt%3DAbstract Authors: Miyajima Y, Satoh K, Umeda Y, Makimura K Small amounts of contaminants may lead to false-positive results in sensitive polymerase chain reaction (PCR) detection systems. To analyze contaminants and understand the usability of beta-glucanases in fungal preparations, we estimated the ribosomal DNA (rDNA) contamination in Zymolyase-100T and Lyticase by quantitative PCR. The amount of rDNA contamination determined by real-time PCR was 9210 copies/unit for Zymolyase-100T and 0.0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary. PMID: 19942798 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037253 Mon, 30 Nov 2009 04:02:03 +0100 3037253 http://www.medworm.com/index.php?rid=3037252&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19942799%26dopt%3DAbstract Authors: Takashima M PMID: 19942799 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=3037252 Mon, 30 Nov 2009 04:02:03 +0100 3037252 http://www.medworm.com/index.php?rid=2769098&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654445%26dopt%3DAbstract Authors: Toyotome T, Watanabe A, Iwasaki A, Kamei K Humans are continually inhaling environmental fungi. When the host immune system is competent, the inhaled fungi are cleared away from the lung by host defense mechanisms. But in immunocompromised individuals, the environmental fungi (e.g., Aspergillus fumigatus) sometimes cause infection. Pathogenic fungi possess various mechanisms to invade the host. A. fumigatus is no exception in possessing several virulence factors and defense mechanisms against host immune attack.One of the virulence factors is secondary metabolite. A. fumigatus produces a variety of secondary metabolites, and the fungal products in culture supernatant have a strong apoptosis-inducing activity to macrophages and alveolar epithelial cells. These data suggest that... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2769098 Sun, 06 Sep 2009 02:00:04 +0100 2769098 http://www.medworm.com/index.php?rid=2679058&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654445%26dopt%3DAbstract Authors: Toyotome T, Watanabe A, Iwasaki A, Kamei K Humans are continually inhaling environmental fungi. When the host immune system is competent, the inhaled fungi are cleared away from the lung by host defense mechanisms. But in immunocompromised individuals, the environmental fungi (e.g., Aspergillus fumigatus) sometimes cause infection. Pathogenic fungi possess various mechanisms to invade the host. A. fumigatus is no exception in possessing several virulence factors and defense mechanisms against host immune attack.One of the virulence factors is secondary metabolite. A. fumigatus produces a variety of secondary metabolites, and the fungal products in culture supernatant have a strong apoptosis-inducing activity to macrophages and alveolar epithelial cells. These data suggest that... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679058 Fri, 07 Aug 2009 15:24:03 +0100 2679058 http://www.medworm.com/index.php?rid=2679057&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654446%26dopt%3DAbstract Authors: Ishibashi Y Malassezia yeasts are part of the cutaneous microflora and are also associated with a number of skin diseases such as pityriasis versicolor, seborrheic dermatitis, and atopic dermatitis (AD). Among organisms of the Malassezia species, M. globosa and M. restricta are highly associated with AD. However, their precise role in AD has remained uncertain. We first attempted to identify major allergens from M. globosa using a proteomics analysis. Immunoblotting showed that IgE-reactive components with molecular masses of 40-45 kDa proteins were detected by 100% (28 of 28) of sera from AD patients. The IgE-reactive allergens corresponding to the 42 kDa protein (MGp42) were identified by two-dimensional immunoblotting, and partially sequenced by MALDI-TOF MS with post sourc... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679057 Fri, 07 Aug 2009 15:24:03 +0100 2679057 http://www.medworm.com/index.php?rid=2679056&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654447%26dopt%3DAbstract Authors: Koga T Infections with dermatophytes are generally confined to the keratinized stratum corneum. This superficial site of infection may protect the infecting dermatophytes from direct contact with some of the effector cells of the immune system; therefore, the immune system has developed a special subsystem in the skin to eliminate them.The innate immunity and acquired immunity (delayed-type hypersensitivity response) are both required for cutaneous immune surveillance against dermatophytes in the skin.Epidermal keratinocytes not only have an important structural role in forming a physical barrier to dermatophytes but also are important functionally in mediating cutaneous immune reactions. These cells can secrete proinflammatory cytokines, chemokines, and anti-microbial peptide... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679056 Fri, 07 Aug 2009 15:24:03 +0100 2679056 http://www.medworm.com/index.php?rid=2679055&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654448%26dopt%3DAbstract We report a case of kerion celsi caused by Microsporum gypseum and present some epidemiological statistics and a distribution of the mating types of M. gypseum . A 10-year-old healthy boy living in Narita, Chiba Prefecture, visited the Narita Red Cross Hospital in October 2004 with complaints of a scaly erythematous plaque and alopecia. Before the visit, he had been treated with steroid lotions and antibiotics without success. A direct examination of the diseased hair shaft using a potassium hydroxide (KOH) solution revealed the presence of fungal hyphae outside the hair shafts. The patient showed a positive reaction to the trichophytin test. The fungus isolated from the lesion was identified as M. gypseum on the basis of its morphological and physiological characteristics and the results ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679055 Fri, 07 Aug 2009 15:24:03 +0100 2679055 http://www.medworm.com/index.php?rid=2679054&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654449%26dopt%3DAbstract Authors: Kunitake Y, Noguchi H, Hiruma M Twelve cases of tinea capitis were encountered at a clinic in Kumamoto prefecture between April 2004 and December 2008. Patients were boys aged between 2 and 18 in 11 cases, and a woman aged 50 years in 1 case. The pathogen was Microsporum canis in 6 cases, and Trichophyton tonsurans in 6 cases. In the cases with M. canis , patients were boys aged 2 to 8 years in 5 cases, and the type of disease was non-inflammatory in 5 cases and inflammatory in 1 case. The patients with T. tonsurans were boys aged 12 to 18 years (judo athletes in 5 cases) and the type of disease was inflammatory in 2 cases and black dot in 4 cases. Three of the M. canis cases were treated with oral administration of itraconazole (ITCZ) at doses of 2.4-4.0 mg/kg/day, and the ot... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679054 Fri, 07 Aug 2009 15:24:03 +0100 2679054 http://www.medworm.com/index.php?rid=2679053&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654450%26dopt%3DAbstract Authors: Adachi H, Shimizu K, Hattori H, Tanaka R, Chibana H, Takagi Y, Tomita Y, Kanbe T Because of its high discriminatory potential, fragment analysis of microsatellites has been frequently used for genotyping of Candida albicans at the strain level. In order to evaluate a genotyping system based on the fragment analysis of microsatellites combined with PCRs targeting 25S rDNA and RPS, 456 independent strains of C. albicans were subjected to genotype analysis using 4 microsatellite markers (CDC3, HIS3, CA I and CA III), followed by 25S rDNA and RPS-based genotyping. The fragment analysis using CA I showed the highest discriminatory potential (DP=0.9782), followed by HIS3 (DP=0.8780). Using combined microsatellite markers, 456 C. albicans strains were divided into 384 genotypes (DP=0... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679053 Fri, 07 Aug 2009 15:24:03 +0100 2679053 http://www.medworm.com/index.php?rid=2679052&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654451%26dopt%3DAbstract Authors: Liu Y, Kang Y, Yokoyama K, Gonoi T, Mikami Y Genetic heterogeneity and drug susceptibility patterns of 43 Candida albicans isolates from patients with respiratory infections in China were compared with those of 52 isolates from Japanese patients. Of the 43 strains, 17 were genotype A, 17 were genotype B, 8 were genotype C, and 1 was genotype E. Drug susceptibilities against amphotericin B, flucytosine, 3 azoles, and micafungin of the 43 strains and the 52 Japanese isolates were compared. All strains in China were susceptible to all drugs tested, and susceptibility patterns of the two countries were similar. Four Japanese isolates showed trailing growth for azole antifungals; Chinese strains did not. PMID: 19654451 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679052 Fri, 07 Aug 2009 15:24:03 +0100 2679052 http://www.medworm.com/index.php?rid=2679051&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19654452%26dopt%3DAbstract Authors: Cho T PMID: 19654452 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2679051 Fri, 07 Aug 2009 15:24:03 +0100 2679051 http://www.medworm.com/index.php?rid=2531658&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430179%26dopt%3DAbstract Authors: Niimi K, Niimi M The echinocandin (candin) class of antifungal drugs inhibit beta-1,3-glucan synthase and block synthesis of beta-1,3-glucan , an important polysaccharide in fungal cell walls. Candins are used widely for treatment of systemic infections caused by Candida and Aspergillus because of their high potency and low toxicity to humans. The incidence of candin resistance has been rare compared to that of azole resistance, although candin-resistant clinical isolates of C. albicans, C. glabrata, C. krusei and C. tropicalis have been reported in the USA and Europe in recent years. These isolates possess hundred-fold higher MIC values for candins than sensitive strains, as well as candin-resistant beta-1,3-glucan synthase activities. Their candin resistance is associated wi... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531658 Sat, 27 Jun 2009 15:33:03 +0100 2531658 http://www.medworm.com/index.php?rid=2531657&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430180%26dopt%3DAbstract Authors: Takahashi T Pneumocystis (Pc) jirovecii causes severe interstitial pneumonia in patients with immunodeficiency, in whom this fungus adheres with type-I alveolar epithelial cells. Therefore, it is important to perform quick diagnosis and treatment for Pc pneumonia (PcP). In general, a combination of two antifolate agents, sulfamethoxazole (inhibition of dihydropteroate synthase (DHPS)) and trimethoprim (inhibition of dihydrofolate reductase), is the first choice for PcP treatment, and pentamidine or atovaquone (inhibition of cytochrome b) are the alternative reagents for the therapy. Amino acid substitutions of drug-binding sites in DHPS shown in genotypic analysis have been reported to be associated with failures of prophylaxis / treatment or severe mortality for PcP, while th... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531657 Sat, 27 Jun 2009 15:33:03 +0100 2531657 http://www.medworm.com/index.php?rid=2531656&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430181%26dopt%3DAbstract Authors: Shinozaki M, Okubo Y, Nakayama H, Mitsuda A, Ide T, Yamagata Murayama S, Shibuya K The present article describes our studies to know the usefulness of in situ hybridization (ISH) to identify various kinds of mold observed in tissue sections and / or cytological preparations from the lesions of patients with invasive fungal infection. To establish the precise procedure for ISH in formalin-fixed and paraffin-embedded sections, various pretreatments were attempted. The condition finally chosen is written here providing a favorable outcome regarding to both intensity and specificity of signals on outline of molds observed in the tissue sections when specimens were treated with both heat and proteinase K and, solutions were adjusted to higher pH value.Therefore, usefulness of promi... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531656 Sat, 27 Jun 2009 15:33:03 +0100 2531656 http://www.medworm.com/index.php?rid=2531655&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430182%26dopt%3DAbstract Authors: Koga H Tinea corporis and the tinea pedis model in guinea pig with Trichophyton mentagrophytes are well established models of dermatophytoses. We attempted to provide animal infection models for T. tonsurans, endemic in Japan, and Malassezia restricta, an important pathogenic factor in seborrhoeic dermatitis, by utilizing the tinea corporis model. An inoculum of the organisms was applied to the back skin of male guinea pigs. T. tonsurans infected animals showed follicular inflammation mimicking those seen in humans. Interestingly, anthropophilic T. tonsurans showed a high infection rate in animal skin. Meanwhile, a single application of M. restricta, as well as consecutive applications to the surface of the skin without any pretreatment, succeeded in producing scales mimicking... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531655 Sat, 27 Jun 2009 15:33:03 +0100 2531655 http://www.medworm.com/index.php?rid=2531654&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430183%26dopt%3DAbstract In this study, the antifungal activities of itraconazole and voriconazole against clinical isolates of C. neoformans, Candida spp., and A. fumigatus isolated from animals with mycoses were examined using the broth microdilution method performed according to the guidelines provided by the Clinical and Laboratory Standards Institute. The minimum inhibitory concentrations (MICs) of itraconazole toward the C. neoformans, Candida spp., and A. fumigatus isolates were 0.125 - 1, 0.125 - 2, and 0.25 - 2 microg/ml, respectively, and those of voriconazole were 0.0625 - 0.5, < or =0.0313 - 0.0625, and 0.0625 - 1 microg/ml, respectively. The results of the MIC analyses implied that the fungal isolates obtained from infected animals exhibit an equivalent degree of susceptibility to itraconazole and ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531654 Sat, 27 Jun 2009 15:33:03 +0100 2531654 http://www.medworm.com/index.php?rid=2531653&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430184%26dopt%3DAbstract Authors: Juhi T, BibhaBati M, Aradhana B, Poonam L, Vinita D, Archana T Seven cases of cryptococcus meningitis in a tertiary care hospital from 2004-2007 were reviewed. 85.7% of the patients had headache as their predominant clinical feature. The spectrum of CT / MR findings ranged from no abnormality, basal ganglion lesion, to intracerebral and intraventricular granulomas. Findings of cerebrospinal fluid (CSF) cytology and biochemistry analysis were inconclusive. Patients were diagnosed by India ink(100%), CSF cryptococcal antigen detection(100%), and CSF culture in 6(85.7%). With the exception of two patients, co-morbidities associated were HIV, diabetes mellitus, and idiopathic CD4 + lymphocytopenia. Six patients were successfully treated with amphotericin B and discharged. A high i... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531653 Sat, 27 Jun 2009 15:33:03 +0100 2531653 http://www.medworm.com/index.php?rid=2531652&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430185%26dopt%3DAbstract Authors: Takenaka M, Sato S, Nishimoto K A total of 155 sporotrichosis cases examined in Nagasaki prefecture, including 138 cases which had been previously reported and 16 examined from 2002 to 2007, were surveyed and compared. No significant differences were found between 143 cases from 1951 to 2001 and 12 of these from 2002 to 2007 in sex or the affected regions of the body. Males and females were equally affected. The lesions were frequently seen on the face(28.2%, 25.0%)and upper limbs (62.1%, 66.7%). Fixed type (62.1%)was much more frequent than the lymphocutaneous type(37.9%)from 1951 to 2001, but in recent year there was an equal number of two types. The rate of patients over 50 years of age increased from 72.1% to 91.7%, and of note was that there were no patients under 10 year... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531652 Sat, 27 Jun 2009 15:33:03 +0100 2531652 http://www.medworm.com/index.php?rid=2531651&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430186%26dopt%3DAbstract Authors: Shiota R, Kaneko T, Yano H, Takeshita K, Nishioka K, Makimura K Malassezia-positive smears can be recognized from otitis externa, however, there are few references in the literature to the relation between Malassezia and otitis externa. Therefore, the bacterial and clinical characteristics of 72 cases (63 patients) with otitis externa were investigated at the Department of Otorhinolaryngology, Takinomiya General Hospital to analyze this. Thirty-seven cases were bacterial otitis externa, 20 cases were fungal otitis externa, and 15 cases were etiological agents unknown in this study. The causative organisms in fungal otitis externa were the genera Aspergillus (10 cases), Malassezia (5) and Candida (5), respectively. We suspected that 5 cases were caused by Malassezia because Mal... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531651 Sat, 27 Jun 2009 15:33:03 +0100 2531651 http://www.medworm.com/index.php?rid=2531650&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430187%26dopt%3DAbstract Authors: Nishimura K PMID: 19430187 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531650 Sat, 27 Jun 2009 15:33:03 +0100 2531650 http://www.medworm.com/index.php?rid=2531649&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19430188%26dopt%3DAbstract Authors: Akiyama K, Taniguchi M PMID: 19430188 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2531649 Sat, 27 Jun 2009 15:33:03 +0100 2531649 http://www.medworm.com/index.php?rid=2166644&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194052%26dopt%3DAbstract Authors: Nishimoto K Dermatophytes are fungi capable of digesting keratin and able to infect the skin surface of animal. Among them, the anthropophilic species Trichophyton rubrum is the most important human pathogen in Japan as the causative species of tinea lesions. The lesions caused by this fungus are known to be mild in their inflammatory reaction. More than 20% of the Japanese population is believed to be suffering from tinea pedis and the situation has not changed despite the introduction of new potent antifungal drugs. Several attempts made to cultivate the fungus on the skin surface has revealed the presence of pathogenic dermatophytes in healthy looking skin around a lesion or on the skin of surrounding individuals. Also, more than half of tinea pedis patients are left untrea... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166644 Sun, 08 Feb 2009 12:48:05 +0100 2166644 http://www.medworm.com/index.php?rid=2166643&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194053%26dopt%3DAbstract Authors: Matsumoto T I (the author), Tadahiko Matsumoto, who is a winner of the 2008 Japanese Society for Medical Mycology (JSMM) Award, was born in 1945 and graduated in 1969 from Kyushu University in Fukuoka, Japan with an M.D. degree. At the Department of Dermatology, Kyushu University I studied dermatology and medical mycology. In Tokyo (1970-1971) at the Department of Microbiology, National Institute of Hygienic Sciences I learned general mycology. During the period from 1981 to 1983 I further studied medical mycology at the Division of Mycotic Diseases (Director: Dr. Libero Ajello), Center for Infectious Diseases, Centers for Disease Control (CDC) in Atlanta, Georgia in the United States. During the period from 1991 to 2005 while working as Director of Dermatology of Toshiba Hosp... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166643 Sun, 08 Feb 2009 12:48:05 +0100 2166643 http://www.medworm.com/index.php?rid=2166642&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194054%26dopt%3DAbstract In this study we found that it was harder to kill T. rubrum than other dermatophytes. Therefore, liranaftate with its potent fungicidal activities was suggested an efficacious agent for the treatment of dermatophytes. PMID: 19194054 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166642 Sun, 08 Feb 2009 12:48:05 +0100 2166642 http://www.medworm.com/index.php?rid=2166641&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194055%26dopt%3DAbstract Authors: Kawasaki M, Anzawa K, Mochizuki T, Ishizaki H, Hemashettar BM An isolate of Arthroderma simii was successfully mated with a tester strain of A. vanbreuseghemii cultured on the plate of simple agar with some hair on it at 27 degrees C. Confirmation of sexual reproduction was made by the detection of hybrids of two parental genotypes. The implications of this result are discussed from the viewpoint of a reevaluation of the species boundaries of dermatophytes. PMID: 19194055 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166641 Sun, 08 Feb 2009 12:48:05 +0100 2166641 http://www.medworm.com/index.php?rid=2166640&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194056%26dopt%3DAbstract Authors: Ishizaki H, Kawasaki M, Anzawa K, Mochizuki T, Chakrabarti A, Ungpakorn R, Torres Guererro H, Toriello C, Arenas R Up to now, 30 mitochondrial DNA(mtDNA)and 4 rDNA types of Sporothrix schenckii strains have been identified. Here, seventy-six isolates of S. schenckii from Mexico, Guatemala, Brazil, Thailand and India were genotyped and studied epidemiologically by mtDNA restriction fragment length polymorphisms(RFLP)and internal transcribed spacer region(ITS)-RFLP analysis and two new mtDNA types, Type 31 and Type 32, were found. Type 30, previously reported by Mora-Cabrera et al. was confirmed to be Type 3 and designated as blank. Of 48 isolates from Mexico, 41 belonged to Group A wherein Type 2(13 isolates), Type 3(10)and Type 28(7)were dominant. All ten isolates from India a... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166640 Sun, 08 Feb 2009 12:48:05 +0100 2166640 http://www.medworm.com/index.php?rid=2166639&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194057%26dopt%3DAbstract Authors: Ashizawa K PMID: 19194057 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=2166639 Sun, 08 Feb 2009 12:48:05 +0100 2166639 http://www.medworm.com/index.php?rid=1967574&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001750%26dopt%3DAbstract Authors: Jigami Y Glycosylphosphatidyl-inositol (GPI) -anchored mannoproteins are one of the major cell wall components of eukaryotic microorganisms, including yeast and fungi. Some GPI-anchored proteins are localized at the plasma membrane, but others are processed at the plasma membrane and are covalently linked to beta-1, 6-glucan of the cell wall through the GPI portion. The genes and enzymes responsible for their biosynthesis and cell wall assembly are potential targets of anti-fungal reagents. We identified GWT1 as a new anti-fungal drug candidate target and elucidated its function as being involved in the acylation of the inositol ring. We also found a new function of GPI7 , which is involved in transfer of ethanolamine phosphate to Man2 of GPI. Our results indicate that the loc... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967574 Wed, 19 Nov 2008 05:34:14 +0100 1967574 http://www.medworm.com/index.php?rid=1967573&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001751%26dopt%3DAbstract Authors: Watanabe A, Hashimoto Y, Higurashi H, Ochiai E, Toyotome T, Nagayoshi M, Kamei K The number of patients with invasive fungal infection (IFI) has dramatically increased since the beginning of the 1980s. Aspergillus fumigatus, the most common species recovered from aspergillosis, is an important pathogen of IFI. Recently, new antifungal agents have become available in Japan, but mortality from aspergillosis is still high. Early initiation of therapy seems to improve the survival rate. Study of virulence factors of the fungus may lead to the development of novel diagnostic tools or advancements in therapy. Many candidates of the fungal virulence factors have been studied including proteases and mycotoxins. We previously discussed the influence of fungal secondary metabolites such... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967573 Wed, 19 Nov 2008 05:34:14 +0100 1967573 http://www.medworm.com/index.php?rid=1967572&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001752%26dopt%3DAbstract Authors: Kimura M A pathological diagnosis can be a decisive diagnosis for deep-seated mycosis. HE stain is used to look for structural changes of the infected lesion and then various special stains are used to visualize the fungus in sections. When viability of the fungus is low, it is faintly stained with HE or PAS. Grocott stain can clearly demonstrate the fungus regardless of viability. Overstaining and understaining sometimes occur. Grocott stain is not suitable to detect structural changes of an infected lesion. Cell walls of Cryptococcus and dematiaceous fungi are stained brown with Fontana-Masson stain because of the existence of melanin. However, it is noteworthy that some Aspergillus and zygomycetes also turn brown with this stain. Fungiflora Y with a fluorescence microscope ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967572 Wed, 19 Nov 2008 05:34:14 +0100 1967572 http://www.medworm.com/index.php?rid=1967571&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001753%26dopt%3DAbstract Authors: Izumikawa K, Kohno S The number of systemic fungal infection (SFI) cases is going due to the increase in immunocompromised hosts associated with advanced chemotherapy and high-tech medical devices. Aspergillus, Candida and Cryptococcus are major etiologies of SFIs and a remarkable increase of aspergillosis has been noted. The problems of SFIs in general are lower diagnostic rate and worse prognosis compared to infections by other pathogens. Clinical concerns of SFIs are the low diagnostic rate of aspergillosis, lack of evidence for treatment of chronic aspergillosis, poor outcome of cryptococcal encephalomeningitis of immunocompromised hosts, and increasing low azole-susceptible Candida. Our final goal is to overcome these problems and to develop a newer rapid diagnostic tool ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967571 Wed, 19 Nov 2008 05:34:14 +0100 1967571 http://www.medworm.com/index.php?rid=1967570&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001754%26dopt%3DAbstract In this report, we discuss the effects of farnesol on: 1. growth of Candida albicans in vitro and in vivo; 2. the incorporation of biomolecules into the cell wall of Candida albicans; and 3. cytokine expression by the immune system. Our results indicate genes of Candida albicans expressed at an early stage of quorum-sensing. Half of these genes are known and two-thirds of known genes are up-regulated by two types of transcription factors. PMID: 19001754 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967570 Wed, 19 Nov 2008 05:34:14 +0100 1967570 http://www.medworm.com/index.php?rid=1967569&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001755%26dopt%3DAbstract Authors: Nagi-Miura N, Adachi Y, Ohno N The intraperitoneal administration of CAWS (water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans NBRC 1385) to mice induces coronaritis similar to Kawasaki disease. We analyzed differences in the occurrence of coronary arteritis among mouse strains, inbred strains, a closed colony, hybrids and mutants. CAWS vasculitis was induced in almost all of the inbred and closed colony strains tested, except for CBA / J mice; it was induced also in hybrids, CDF1 and BDF1. In mutant strains of various immunological defects, such as C57BL / 6J Ham Slc-bg , Balb / c nu / nu , C.B.17 / Icr-scid / scid , WBB6F1-W / W (v) mice, all induced CAWS vasculitis but a relatively weak phenotype. It has already been... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967569 Wed, 19 Nov 2008 05:34:14 +0100 1967569 http://www.medworm.com/index.php?rid=1967568&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001756%26dopt%3DAbstract Authors: Yamada T Molecular biological studies of the host invasion mechanisms and possible virulence-related factors of dermatophytes have just begun. The identification of individual genes and large-scale investigations of transcripts expressed under different experimental culture conditions have provided useful information on the structure, expression, and regulation of the genes of major dermatophyte species such as Trichophyton rubrum. The next goal of dermatophytosis research will be to elucidate the functions and roles of the identified fungal genes during the infection process. It will also be necessary to investigate the host immune responses to fungal gene expression and regulation during infection. For such research, genetic manipulation techniques for dermatophytes, such as... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967568 Wed, 19 Nov 2008 05:34:14 +0100 1967568 http://www.medworm.com/index.php?rid=1967567&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001758%26dopt%3DAbstract Authors: Shinoda H, Nishimoto K, Mochizuki T This paper presents the results of an examination for Trichophyton tonsurans(T. tonsurans)performed by the hairbrush (HB; 90 bristles)method at the All Japan Inter High School Championships, Saga 2007. Samples were taken from 487 Judo practitioners (265 males and 222 females) out of a total of 951. The areas with the highest positive rates were: Kyushu 21%(15 participants out of 73 sampled), Tohoku 17% (13 out of 77), Kinki 16% (14 out of 89), and Chubu 13% (12 out of 89). Four participants from Kyushu, four from Tohoku, two from Kinki, and two from Chubu were strongly positive carriers, their samples developing more than 30 colonies per dish. This finding is in concordance with the high HB-positive rates in these areas. The results of a que... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967567 Wed, 19 Nov 2008 05:34:14 +0100 1967567 http://www.medworm.com/index.php?rid=1967566&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001760%26dopt%3DAbstract Authors: Kobayashi M, Kabashima K, Tokura Y Dermatophytes reside in the stratum corneum of the epidermis, and one scenario in superficial dermatophytosis is that fungi stimulate keratinocytes to secrete chemokines, thereby attracting inflammatory cells. We investigated the effect of the cytokine / chemokine production of keratinocytes solely stimulated by fungal elements. The fungal elements beta-D-glucan and trichophytin from Trichophyton rubrum and Trichophyton mentagrophytes augmented production of IL-8 and IL-1 alpha of cultured normal human epidermal keratinocytes. It was found that keratinocytes can recognize elements of dermatophytes as a pathogen. Next we examined the effect of liranaftate, a representative Japanese thiocarbamate antifungal agent, on the production of IL-8 and ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967566 Wed, 19 Nov 2008 05:34:14 +0100 1967566 http://www.medworm.com/index.php?rid=1967565&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001761%26dopt%3DAbstract Authors: Kanda Y PMID: 19001761 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967565 Wed, 19 Nov 2008 05:34:14 +0100 1967565 http://www.medworm.com/index.php?rid=1967564&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001762%26dopt%3DAbstract Authors: Maruyama R PMID: 19001762 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1967564 Wed, 19 Nov 2008 05:34:14 +0100 1967564 http://www.medworm.com/index.php?rid=1959123&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001749%26dopt%3DAbstract Authors: Zakikhany K, Thewes S, Wilson D, Martin R, Albrecht A, Hube B PMID: 19001749 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959123 Sat, 15 Nov 2008 06:51:19 +0100 1959123 http://www.medworm.com/index.php?rid=1959122&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001750%26dopt%3DAbstract Authors: Jigami Y Glycosylphosphatidyl-inositol (GPI) -anchored mannoproteins are one of the major cell wall components of eukaryotic microorganisms, including yeast and fungi. Some GPI-anchored proteins are localized at the plasma membrane, but others are processed at the plasma membrane and are covalently linked to beta-1, 6-glucan of the cell wall through the GPI portion. The genes and enzymes responsible for their biosynthesis and cell wall assembly are potential targets of anti-fungal reagents. We identified GWT1 as a new anti-fungal drug candidate target and elucidated its function as being involved in the acylation of the inositol ring. We also found a new function of GPI7 , which is involved in transfer of ethanolamine phosphate to Man2 of GPI. Our results indicate that the loc... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959122 Sat, 15 Nov 2008 06:51:19 +0100 1959122 http://www.medworm.com/index.php?rid=1959121&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001751%26dopt%3DAbstract Authors: Watanabe A, Hashimoto Y, Higurashi H, Ochiai E, Toyotome T, Nagayoshi M, Kamei K The number of patients with invasive fungal infection (IFI) has dramatically increased since the beginning of the 1980s. Aspergillus fumigatus, the most common species recovered from aspergillosis, is an important pathogen of IFI. Recently, new antifungal agents have become available in Japan, but mortality from aspergillosis is still high. Early initiation of therapy seems to improve the survival rate. Study of virulence factors of the fungus may lead to the development of novel diagnostic tools or advancements in therapy. Many candidates of the fungal virulence factors have been studied including proteases and mycotoxins. We previously discussed the influence of fungal secondary metabolites such... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959121 Sat, 15 Nov 2008 06:51:19 +0100 1959121 http://www.medworm.com/index.php?rid=1959120&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001752%26dopt%3DAbstract Authors: Kimura M A pathological diagnosis can be a decisive diagnosis for deep-seated mycosis. HE stain is used to look for structural changes of the infected lesion and then various special stains are used to visualize the fungus in sections. When viability of the fungus is low, it is faintly stained with HE or PAS. Grocott stain can clearly demonstrate the fungus regardless of viability. Overstaining and understaining sometimes occur. Grocott stain is not suitable to detect structural changes of an infected lesion. Cell walls of Cryptococcus and dematiaceous fungi are stained brown with Fontana-Masson stain because of the existence of melanin. However, it is noteworthy that some Aspergillus and zygomycetes also turn brown with this stain. Fungiflora Y with a fluorescence microscope ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959120 Sat, 15 Nov 2008 06:51:19 +0100 1959120 http://www.medworm.com/index.php?rid=1959119&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001753%26dopt%3DAbstract Authors: Izumikawa K, Kohno S The number of systemic fungal infection (SFI) cases is going due to the increase in immunocompromised hosts associated with advanced chemotherapy and high-tech medical devices. Aspergillus, Candida and Cryptococcus are major etiologies of SFIs and a remarkable increase of aspergillosis has been noted. The problems of SFIs in general are lower diagnostic rate and worse prognosis compared to infections by other pathogens. Clinical concerns of SFIs are the low diagnostic rate of aspergillosis, lack of evidence for treatment of chronic aspergillosis, poor outcome of cryptococcal encephalomeningitis of immunocompromised hosts, and increasing low azole-susceptible Candida. Our final goal is to overcome these problems and to develop a newer rapid diagnostic tool ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959119 Sat, 15 Nov 2008 06:51:19 +0100 1959119 http://www.medworm.com/index.php?rid=1959118&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001754%26dopt%3DAbstract In this report, we discuss the effects of farnesol on: 1. growth of Candida albicans in vitro and in vivo; 2. the incorporation of biomolecules into the cell wall of Candida albicans; and 3. cytokine expression by the immune system. Our results indicate genes of Candida albicans expressed at an early stage of quorum-sensing. Half of these genes are known and two-thirds of known genes are up-regulated by two types of transcription factors. PMID: 19001754 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959118 Sat, 15 Nov 2008 06:51:19 +0100 1959118 http://www.medworm.com/index.php?rid=1959117&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001755%26dopt%3DAbstract Authors: Nagi-Miura N, Adachi Y, Ohno N The intraperitoneal administration of CAWS (water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans NBRC 1385) to mice induces coronaritis similar to Kawasaki disease. We analyzed differences in the occurrence of coronary arteritis among mouse strains, inbred strains, a closed colony, hybrids and mutants. CAWS vasculitis was induced in almost all of the inbred and closed colony strains tested, except for CBA / J mice; it was induced also in hybrids, CDF1 and BDF1. In mutant strains of various immunological defects, such as C57BL / 6J Ham Slc-bg , Balb / c nu / nu , C.B.17 / Icr-scid / scid , WBB6F1-W / W (v) mice, all induced CAWS vasculitis but a relatively weak phenotype. It has already been... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959117 Sat, 15 Nov 2008 06:51:19 +0100 1959117 http://www.medworm.com/index.php?rid=1959116&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001756%26dopt%3DAbstract Authors: Yamada T Molecular biological studies of the host invasion mechanisms and possible virulence-related factors of dermatophytes have just begun. The identification of individual genes and large-scale investigations of transcripts expressed under different experimental culture conditions have provided useful information on the structure, expression, and regulation of the genes of major dermatophyte species such as Trichophyton rubrum . The next goal of dermatophytosis research will be to elucidate the functions and roles of the identified fungal genes during the infection process. It will also be necessary to investigate the host immune responses to fungal gene expression and regulation during infection. For such research, genetic manipulation techniques for dermatophytes, such a... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959116 Sat, 15 Nov 2008 06:51:19 +0100 1959116 http://www.medworm.com/index.php?rid=1959115&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001757%26dopt%3DAbstract Authors: Sangetha S, Zuraini Z, Sasidharan S, Suryani S The fungicidal activity of Cassia spectabilis leaf extracts was investigated using the disk diffusion technique and the broth dilution method. The extract showed a favorable antimicrobial activity against Candida albicans with a minimum inhibition concentration(MIC)value of 6.25 mg / ml. Apart from the fungicidal effects, imaging using scanning electron microscopy(SEM)was done to determine the major alterations in the microstructure of the C. albicans. The main abnormalities noted in the SEM studies were the alterations in morphology and complete collapse of the yeast cells after 36 h of exposure to the extract. The in vitro time-kill study performed using the leaf extract at 1 / 2, 1 or 2 times of the MIC significantly inhibited ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959115 Sat, 15 Nov 2008 06:51:19 +0100 1959115 http://www.medworm.com/index.php?rid=1959114&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001758%26dopt%3DAbstract Authors: Shinoda H, Nishimoto K, Mochizuki T This paper presents the results of an examination for Trichophyton tonsurans(T. tonsurans)performed by the hairbrush(HB; 90 bristles)method at the All Japan Inter High School Championships, Saga 2007. Samples were taken from 487 Judo practitioners(265 males and 222 females)out of a total of 951. The areas with the highest positive rates were: Kyushu 21%(15 participants out of 73 sampled), Tohoku 17%(13 out of 77), Kinki 16%(14 out of 89), and Chubu 13%(12 out of 89). Four participants from Kyushu, four from Tohoku, two from Kinki, and two from Chubu were strongly positive carriers, their samples developing more than 30 colonies per dish. This finding is in concordance with the high HB-positive rates in these areas. The results of a questionn... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959114 Sat, 15 Nov 2008 06:51:19 +0100 1959114 http://www.medworm.com/index.php?rid=1959113&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001759%26dopt%3DAbstract Authors: Kawasaki M, Anzawa K, Wakasa A, Takeda K, Tanabe H, Mochizuki T, Ishizaki H, M Hemashettar B To determine the relationships among Trichophyton species we constructed three phylogenies, based on the nucleotide sequences of the actin, rRNA and DNA topoisomerase II genes. These phylogenies showed several conflicting branch points. For example, strains of T. verrucosum,T. concentricum and T. mentagrophytes var. erinacei were mingled with strains of Arthroderma benhamiae and could not be separated into their own phylogenic groups. In addition, strains of A. vanbreuseghemii, T. tonsurans, T. mentagrophytes var. interdigitale and T. mentagrophytes var. quinckeanum were mingled with strains of A. simii and could not be separated into their own phylogenic groups. T. rubrum and T. viola... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959113 Sat, 15 Nov 2008 06:51:19 +0100 1959113 http://www.medworm.com/index.php?rid=1959112&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001760%26dopt%3DAbstract Authors: Kobayashi M, Kabashima K, Tokura Y Dermatophytes reside in the stratum corneum of the epidermis, and one scenario in superficial dermatophytosis is that fungi stimulate keratinocytes to secrete chemokines, thereby attracting inflammatory cells. We investigated the effect of the cytokine / chemokine production of keratinocytes solely stimulated by fungal elements. The fungal elements beta -D-glucan and trichophytin from Trichophyton rubrum and Trichophyton mentagrophytes augmented production of IL-8 and IL-1 alpha of cultured normal human epidermal keratinocytes. It was found that keratinocytes can recognize elements of dermatophytes as a pathogen. Next we examined the effect of liranaftate, a representative Japanese thiocarbamate antifungal agent, on the production of IL-8 and... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959112 Sat, 15 Nov 2008 06:51:19 +0100 1959112 http://www.medworm.com/index.php?rid=1959111&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001761%26dopt%3DAbstract Authors: Kanda Y PMID: 19001761 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959111 Sat, 15 Nov 2008 06:51:19 +0100 1959111 http://www.medworm.com/index.php?rid=1959110&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19001762%26dopt%3DAbstract Authors: Maruyama R PMID: 19001762 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1959110 Sat, 15 Nov 2008 06:51:19 +0100 1959110 http://www.medworm.com/index.php?rid=1707850&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689962%26dopt%3DAbstract Authors: Mikami Y Sequence information of 25S rRNA gene was useful for the genotype determination in Candida albicans. Genotypes of 301 C. albicans strains by this single PCR method were determined, and out of them, two strains of new genotype (genotype E) which are closely related to C. dubliniensis in its intron structure were discovered. Analysis of internal transcribed spacer (ITS) region sequence including 5.8S rRNA region in three varieties of C. neoformans was found to be an useful method for genotype determination, and a new genotype (Africa genotype) of var. gattii was discovered. In comparison with other traditional taxonomic methods in pathogenic fungi, usefulness of these genotype determination methods in their epidemiological studies was discussed. New PCR identification s... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707850 Sun, 17 Aug 2008 09:30:10 +0100 1707850 http://www.medworm.com/index.php?rid=1707849&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689965%26dopt%3DAbstract Authors: Katoh T We provide foot and nail care services to outpatients at our dermatology clinic in Saiseikai Kawaguchi General Hospital. Patients with dermatological foot problems such as onychogryphosis, onychomycosis, and ingrown nails are recommended by dermatologists to obtain foot and nail care services performed by a specially trained nurse. These services include a footbath, foot massage, nail clipping, and corn and nail filing. If ingrown nails exist, a small piece of cloth is placed under the nail edge. PMID: 18689965 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707849 Sun, 17 Aug 2008 09:30:10 +0100 1707849 http://www.medworm.com/index.php?rid=1707848&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689966%26dopt%3DAbstract Authors: Uno T Fungal keratitis is one of the most challenging types of microbial keratitis for the ophthalmologist to diagnose and treat. Fungi causing human keratitis take the form of either yeasts or mold. Candida, the major pathogenic yeasts, can be detected in the normal ocular surface flora. Preceding ocular surface disorder, the wearing of contact lenses and the use of antibiotic/steroid eye drops may lead to candida keratitis. Infectious focus caused by Candida tends to melt the corneal stroma. Keratitis caused by mold often develops after an injury caused by soil and/or a plant. Mold can reach the anterior chamber without destroying the stromal layer of the cornea, which results in distinctive clinical features such as endothelial plaque and hyphate ulcer. Fungal keratitis nee... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707848 Sun, 17 Aug 2008 09:30:10 +0100 1707848 http://www.medworm.com/index.php?rid=1707847&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689967%26dopt%3DAbstract Authors: Mochizuki T In July 2007, information on the current status of mycological examinations in Japanese university clinics and requests for a university network system was gathered by a questionnaire completed by 98 of 112 professors or directors of those clinics. A summary of the findings follows: only 9% of the hospitals performed fungal culture studies for all or most cases, indicating a drop of 7% from 2000 (16% in 2000, reported by Kasai) . Also, just 55% of relevant departments maintained the ability to identify most or all clinical isolates in-house, which down 27% from 2000 (83% in 2000, reported by Kasai). These findings indicate that mycological diagnoses by many departments have been rapidly decreasing. Eighty-nine percent of respondents indicated a desire for some help... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707847 Sun, 17 Aug 2008 09:30:10 +0100 1707847 http://www.medworm.com/index.php?rid=1707846&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689968%26dopt%3DAbstract Authors: Kamei K Due to the serious financial condition of the health care insurance system in Japan, many clinical microbiology laboratories in hospitals have been forced to close or downsize, and therefore identification of pathogenic fungi isolated in these hospitals has become more and more difficult. This problem becomes even more serious when rare but clinically important fungi are the causative agents. For the smooth and accurate identification of the fungi, formation of a collaborative network among hospital laboratories, private clinical laboratory test centers and university research laboratories is now required. In contrast, the culture collection system of pathogenic fungi for deposit and distribution has been significantly improved in the past few years largely due to the ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707846 Sun, 17 Aug 2008 09:30:10 +0100 1707846 http://www.medworm.com/index.php?rid=1707845&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689969%26dopt%3DAbstract Authors: Fujihiro M Trichophyton tonsurans infection was found first in autumn 2000 in Gifu prefecture and spread rapidly in the Tokai region. Not only direct KOH examination but also culture is necessary to diagnose this disease. In order to collect a specimen, I recommend cellophane adhesive tape. During the past 5 years, dermatologists in the Tokai area have sent me specimens in an envelope for mycological examination. Hyphae were found in all 75 cases in the scales examined. Fungal culture revealed 61 cases to be T. tonsurans infection; the male : female ratio was 54 : 7. By age distribution, high school students accounted for 46 (75%), elderly patients 9 (15%) and lower age 6 (10%). Judo players accounted for 32 (52%), wrestlers for 24 (39%) and others for 5 (8%). Most had lesions... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707845 Sun, 17 Aug 2008 09:30:10 +0100 1707845 http://www.medworm.com/index.php?rid=1707844&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689970%26dopt%3DAbstract Authors: Hirose N, Suganami M, Shiraki Y, Hiruma M It has been seven years since an outbreak of Trichophyton tonsurans infection occurred in Japan. We have examined the state of T. tonsurans infection by a hairbrush test of athletes and a questionnaire survey of doctors' experience in its treatment, but have not completely grasped the actual state of infection at the sites of judo matches and its treatment or application of preventive measures. SUBJECTS AND METHODS: The questionnaire was distributed to the leaders of all judo clubs (10,077 clubs) registered under the All Japan Judo Federation, and responses recovered from 1,199 clubs (11.9%) were analyzed. These leaders were asked about (1) members of the club, (2) recognition of the infection, (3) experience of the breakout of infecti... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707844 Sun, 17 Aug 2008 09:30:10 +0100 1707844 http://www.medworm.com/index.php?rid=1707843&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689971%26dopt%3DAbstract Authors: Uehara M, Sano A, Yarita K, Kamei K, Haketa M, Ide K, Nagai K, Takayama Y, Nishimura K Penicillium marneffei was isolated from three blood cultures of a Thai woman with AIDS and then identified as such. The patient, 41 a year-old female from northeast Thailand came to Japan 10 years ago and married a Japanese man. She was reportedly the third patient infected with this fungal species in Japan, and considered to be the first case from whom the causative fungus was successfully cultured, which led to the diagnosis of penicilliosis marneffei. The colony of the isolate, which was cultured on Sabouraud dextrose agar at 25-27 degrees C, was initially white and pannose, gradually turned in color from yellow to yellow-green, and diffused a deep red pigment into the medium. Conidial he... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707843 Sun, 17 Aug 2008 09:30:10 +0100 1707843 http://www.medworm.com/index.php?rid=1707842&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689972%26dopt%3DAbstract We report a case of inflammatory tinea corporis by Epidermophyton ( E. ) floccosum. A 73-year-old man visited our hospital with an eruption on his left arm where he wore his wristwatch. He had treated himself with a topical steroid. On KOH preparation, many hyphal elements were observed in the scale. Histological findings revealed granulomatous inflammation around hair follicles and positive fungal elements by PAS and Grocott staining in these follicles. Topical luliconazole and oral terbinafin were effective clinically and mycologically. Epidemiological survey in Japan has shown the number of cases of tinea caused by E. floccosum to be on the decrease recently. The anthropophilic dermatophyte, E. floccosum is known usually not to invade the hair follicle, and no case of inflammatory tinea... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707842 Sun, 17 Aug 2008 09:30:10 +0100 1707842 http://www.medworm.com/index.php?rid=1707841&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689973%26dopt%3DAbstract We report a case of nail candidiasis with severe deformities. The patient was a 71-year-old woman who initially consulted our department on April 5, 2006. She had diabetes, chronic rheumatoid arthritis and multiple liver metastasis of unknown origin. She had taken prednisolone for treatment of chronic rheumatoid arthritis for a long period. The initial examination demonstrated deformation of 1/3 of the inner part of the nail plate in both the third and fourth fingers, with apparent hyperkeratosis under the deformed nail plates. KOH-prepared direct microscopy revealed the presence of numerous spores and pseudohyphae. Numerous fungal elements were detected by Grocott staining and PAS staining. Candida albicans was isolated and identified by cultivation on the ATG agar and PCR-RFLP. Fluconazo... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707841 Sun, 17 Aug 2008 09:30:10 +0100 1707841 http://www.medworm.com/index.php?rid=1707840&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689974%26dopt%3DAbstract Authors: Yaguchi T, Takashima M, Kawasaki H, Makimura K PMID: 18689974 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707840 Sun, 17 Aug 2008 09:30:10 +0100 1707840 http://www.medworm.com/index.php?rid=1707839&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689975%26dopt%3DAbstract Authors: Takakura S PMID: 18689975 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1707839 Sun, 17 Aug 2008 09:30:10 +0100 1707839 http://www.medworm.com/index.php?rid=1699690&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689962%26dopt%3DAbstract Authors: Mikami Y Sequence information of 25S rRNA gene was useful for the genotype determination in Candida albicans. Genotypes of 301 C. albicans strains by this single PCR method were determined, and out of them, two strains of new genotype (genotype E) which are closely related to C. dubliniensis in its intron structure were discovered. Analysis of internal transcribed spacer (ITS) region sequence including 5.8S rRNA region in three varieties of C. neoformans was found to be an useful method for genotype determination, and a new genotype (Africa genotype) of var. gattii was discovered. In comparison with other traditional taxonomic methods in pathogenic fungi, usefulness of these genotype determination methods in their epidemiological studies was discussed. New PCR identification s... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699690 Wed, 13 Aug 2008 09:29:50 +0100 1699690 http://www.medworm.com/index.php?rid=1699689&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689963%26dopt%3DAbstract Authors: Qiao J, Liu W, Li R The incidence of invasive aspergillosis (IA) , which is commonly caused by Aspergillus fumigatus, has increased recently in immunocompromised patients and has become the common cause of death in these patients. Antifungal resistance is one of the reasons for treatment failure. Since the first itraconazole-resistant A. fumigatus was reported in 1997, the reports on clinical strains of triazole-resistant A. fumigatus have increased, as well as studies on the resistant mechanisms. In this paper, the known molecular mechanisms of antifungal resistance in Aspergillus, especially in A. fumigatus, are reviewed. PMID: 18689963 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699689 Wed, 13 Aug 2008 09:29:50 +0100 1699689 http://www.medworm.com/index.php?rid=1699688&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689964%26dopt%3DAbstract Authors: Chakrabarti A, Chatterjee SS, Shivaprakash M In recent years fungi have been flourishing in immunocompromised patients of tertiary care centers. The data on the burden of opportunistic mycoses in India is not clear though the climate in this country is well suited for a wide variety of fungal infections. There are very few good diagnostic mycology laboratories and clinicians are still not aware of the emerging trends. Within the limited data available, an increased incidence of invasive candidiasis, aspergillosis, and zygomycosis are reported. The emergence of fungal rhinosinusitis, penicilliosis marneffei and zygomycosis due to Apophysomyces elegans is unique in the Indian scenario. Invasive candidiasis is the most common opportunistic mycosis. The global change in spectrum o... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699688 Wed, 13 Aug 2008 09:29:50 +0100 1699688 http://www.medworm.com/index.php?rid=1699687&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689965%26dopt%3DAbstract Authors: Katoh T We provide foot and nail care services to outpatients at our dermatology clinic in Saiseikai Kawaguchi General Hospital. Patients with dermatological foot problems such as onychogryphosis, onychomycosis, and ingrown nails are recommended by dermatologists to obtain foot and nail care services performed by a specially trained nurse. These services include a footbath, foot massage, nail clipping, and corn and nail filing. If ingrown nails exist, a small piece of cloth is placed under the nail edge. PMID: 18689965 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699687 Wed, 13 Aug 2008 09:29:50 +0100 1699687 http://www.medworm.com/index.php?rid=1699686&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689966%26dopt%3DAbstract Authors: Uno T Fungal keratitis is one of the most challenging types of microbial keratitis for the ophthalmologist to diagnose and treat. Fungi causing human keratitis take the form of either yeasts or mold. Candida, the major pathogenic yeasts, can be detected in the normal ocular surface flora. Preceding ocular surface disorder, the wearing of contact lenses and the use of antibiotic/steroid eye drops may lead to candida keratitis. Infectious focus caused by Candida tends to melt the corneal stroma. Keratitis caused by mold often develops after an injury caused by soil and/or a plant. Mold can reach the anterior chamber without destroying the stromal layer of the cornea, which results in distinctive clinical features such as endothelial plaque and hyphate ulcer. Fungal keratitis nee... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699686 Wed, 13 Aug 2008 09:29:50 +0100 1699686 http://www.medworm.com/index.php?rid=1699685&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689967%26dopt%3DAbstract Authors: Mochizuki T In July 2007, information on the current status of mycological examinations in Japanese university clinics and requests for a university network system was gathered by a questionnaire completed by 98 of 112 professors or directors of those clinics. A summary of the findings follows: only 9% of the hospitals performed fungal culture studies for all or most cases, indicating a drop of 7% from 2000 (16% in 2000, reported by Kasai) . Also, just 55% of relevant departments maintained the ability to identify most or all clinical isolates in-house, which down 27% from 2000 (83% in 2000, reported by Kasai) . These findings indicate that mycological diagnoses by many departments have been rapidly decreasing. Eighty-nine percent of respondents indicated a desire for some hel... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699685 Wed, 13 Aug 2008 09:29:50 +0100 1699685 http://www.medworm.com/index.php?rid=1699684&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689968%26dopt%3DAbstract Authors: Kamei K Due to the serious financial condition of the health care insurance system in Japan, many clinical microbiology laboratories in hospitals have been forced to close or downsize, and therefore identification of pathogenic fungi isolated in these hospitals has become more and more difficult. This problem becomes even more serious when rare but clinically important fungi are the causative agents. For the smooth and accurate identification of the fungi, formation of a collaborative network among hospital laboratories, private clinical laboratory test centers and university research laboratories is now required. In contrast, the culture collection system of pathogenic fungi for deposit and distribution has been significantly improved in the past few years largely due to the ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699684 Wed, 13 Aug 2008 09:29:50 +0100 1699684 http://www.medworm.com/index.php?rid=1699683&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689969%26dopt%3DAbstract Authors: Fujihiro M Trichophyton tonsurans infection was found first in autumn 2000 in Gifu prefecture and spread rapidly in the Tokai region. Not only direct KOH examination but also culture is necessary to diagnose this disease. In order to collect a specimen, I recommend cellophane adhesive tape. During the past 5 years, dermatologists in the Tokai area have sent me specimens in an envelope for mycological examination. Hyphae were found in all 75 cases in the scales examined. Fungal culture revealed 61 cases to be T. tonsurans infection; the male : female ratio was 54 : 7. By age distribution, high school students accounted for 46 (75%) , elderly patients 9 (15%) and lower age 6 (10%) . Judo players accounted for 32 (52%) , wrestlers for 24 (39%) and others for 5 (8%) . Most had les... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699683 Wed, 13 Aug 2008 09:29:50 +0100 1699683 http://www.medworm.com/index.php?rid=1699682&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689970%26dopt%3DAbstract Authors: Hirose N, Suganami M, Shiraki Y, Hiruma M It has been seven years since an outbreak of Trichophyton tonsurans infection occurred in Japan. We have examined the state of T. tonsurans infection by a hairbrush test of athletes and a questionnaire survey of doctors' experience in its treatment, but have not completely grasped the actual state of infection at the sites of judo matches and its treatment or application of preventive measures. Subjects and Methods: The questionnaire was distributed to the leaders of all judo clubs (10,077 clubs) registered under the All Japan Judo Federation, and responses recovered from 1,199 clubs (11.9%) were analyzed. These leaders were asked about (1) members of the club, (2) recognition of the infection, (3) experience of the breakout of infecti... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699682 Wed, 13 Aug 2008 09:29:50 +0100 1699682 http://www.medworm.com/index.php?rid=1699681&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689971%26dopt%3DAbstract Authors: Uehara M, Sano A, Yarita K, Kamei K, Haketa M, Ide K, Nagai K, Takayama Y, Nishimura K Penicillium marneffei was isolated from three blood cultures of a Thai woman with AIDS and then identified as such. The patient, 41 a year-old female from northeast Thailand came to Japan 10 years ago and married a Japanese man. She was reportedly the third patient infected with this fungal species in Japan, and considered to be the first case from whom the causative fungus was successfully cultured, which led to the diagnosis of penicilliosis marneffei. The colony of the isolate, which was cultured on Sabouraud dextrose agar at 25-27 masculineC, was initially white and pannose, gradually turned in color from yellow to yellow-green, and diffused a deep red pigment into the medium. Conidial h... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699681 Wed, 13 Aug 2008 09:29:50 +0100 1699681 http://www.medworm.com/index.php?rid=1699680&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689972%26dopt%3DAbstract We report a case of inflammatory tinea corporis by Epidermophyton ( E. ) floccosum. A 73-year-old man visited our hospital with an eruption on his left arm where he wore his wristwatch. He had treated himself with a topical steroid. On KOH preparation, many hyphal elements were observed in the scale. Histological findings revealed granulomatous inflammation around hair follicles and positive fungal elements by PAS and Grocott staining in these follicles. Topical luliconazole and oral terbinafin were effective clinically and mycologically. Epidemiological survey in Japan has shown the number of cases of tinea caused by E. floccosum to be on the decrease recently. The anthropophilic dermatophyte, E. floccosum is known usually not to invade the hair follicle, and no case of inflammatory tinea... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699680 Wed, 13 Aug 2008 09:29:50 +0100 1699680 http://www.medworm.com/index.php?rid=1699679&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689973%26dopt%3DAbstract We report a case of nail candidiasis with severe deformities. The patient was a 71-year-old woman who initially consulted our department on April 5, 2006. She had diabetes, chronic rheumatoid arthritis and multiple liver metastasis of unknown origin. She had taken prednisolone for treatment of chronic rheumatoid arthritis for a long period. The initial examination demonstrated deformation of 1/3 of the inner part of the nail plate in both the third and fourth fingers, with apparent hyperkeratosis under the deformed nail plates. KOH-prepared direct microscopy revealed the presence of numerous spores and pseudohyphae. Numerous fungal elements were detected by Grocott staining and PAS staining. Candida albicans was isolated and identified by cultivation on the ATG agar and PCR-RFLP. Fluconazo... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699679 Wed, 13 Aug 2008 09:29:50 +0100 1699679 http://www.medworm.com/index.php?rid=1699678&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689974%26dopt%3DAbstract Authors: Yaguchi T, Takashima M, Kawasaki H, Makimura K PMID: 18689974 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699678 Wed, 13 Aug 2008 09:29:50 +0100 1699678 http://www.medworm.com/index.php?rid=1699677&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18689975%26dopt%3DAbstract Authors: Takakura S PMID: 18689975 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1699677 Wed, 13 Aug 2008 09:29:50 +0100 1699677 http://www.medworm.com/index.php?rid=1667846&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451587%26dopt%3DAbstract [Problems and recent advances in measurement of (1-->3)-beta-D-glucan] Nippon Ishinkin Gakkai Zasshi. 2008;49(2):75-80 Authors: Yoshida K, Shoji H, Niki Y Measurement of (1-->3)-beta-D-glucan for invasive fungal infection is used practically in Japan. The problem of false positive results due to the frequent occurrence of non-specific reaction in alkaline treatment, chromogenic automated kinetic assay to measure (1-->3)-beta-D-glucan had been recognized in Japan. But this important problem was resolved in July 2005 by improvement made in the pretreatment reagent in a (1-->3)-beta-D-glucan measurement kit. In this manuscript, we describe the process of improvement of this kit and its clinical usefulness. PMID: 18451587 [PubMed - indexed for MEDLINE] (Source: Nipp... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667846 Thu, 31 Jul 2008 08:23:38 +0100 1667846 http://www.medworm.com/index.php?rid=1667845&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451588%26dopt%3DAbstract Authors: Kitami Y, Kagawa S, Iijima M A 72 year-old man was referred to our department with white curd-like material on the surface of his tongue as well as the mucosal surface of the lower lip, after unsuccessful treatment with itraconazole for 3 weeks. He also had a history of depression and had received topical steroid and/or antibiotics treatment for persistent oral aphtha and irritation of his upper lip for 4 years. A diagnosis of oral candidiasis was made through positive KOH direct microscopic examination and he was instructed to rinse his oral mucosal lesion with amphotericin B syrup. Although no significant eruption was observed on his upper lip at his first visit, he applied the steroid ointment for 4 weeks and came back to our clinic with his upper lip red and swollen. It wa... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667845 Thu, 31 Jul 2008 08:23:38 +0100 1667845 http://www.medworm.com/index.php?rid=1667844&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451592%26dopt%3DAbstract Authors: Abe M, Ogawa Z, Tanuma H, Yamazaki T, Kume H As there is not yet a standardized in vitro susceptibility test of micafungin (MCFG), we evaluated the methods of such testing, focusing on the judgment method of MIC, based on the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2, M38-A, the proposed standards of The Japanese Society for Medical Mycology (JSMM) for yeast (JSMM-Y) and for filamentous fungi (JSMM-F) against Candida spp. and Aspergillus spp. The judgment of MIC value was performed spectrophotometrically and visually in both (NCCLS and JSMM) assays. Only the spectrophotometric MIC judgment against Aspergillus spp. in the NCCLS assay used two end points: 80% inhibitory concentration (IC80) of the growth control and 50% inhibitory concentration (IC50). ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667844 Thu, 31 Jul 2008 08:23:38 +0100 1667844 http://www.medworm.com/index.php?rid=1667843&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451593%26dopt%3DAbstract We report a case of cutaneous P. boydii infection of the left knee in a 79-year-old Japanese man who was receiving oral predonisolone (25 mg/day) for radiation pneumonitis after radiation therapy on left breast cancer. He presented with a 2-week-history of a lesion on the left knee. A biopsy specimen from the skin lesion revealed granulomatous inflammation with hyphae. Culture of the pus from the skin specimen confirmed the diagnosis of cutaneous P. boydii infection. rDNA ITS sequence was analyzed to confirm the mycological diagnosis. The patient was treated orally with 200 mg/day of itraconazole. The lesion was gradually cured and left a hypertrophic scar. Cutaneous injury may be responsible for an incidence of localized infection. Such rare fungus infection in immunocompromised patients ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667843 Thu, 31 Jul 2008 08:23:38 +0100 1667843 http://www.medworm.com/index.php?rid=1667842&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451595%26dopt%3DAbstract Authors: Nakayama H, Aoyama T PMID: 18451595 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667842 Thu, 31 Jul 2008 08:23:38 +0100 1667842 http://www.medworm.com/index.php?rid=1667841&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451596%26dopt%3DAbstract Authors: Tokimatsu I PMID: 18451596 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1667841 Thu, 31 Jul 2008 08:23:38 +0100 1667841 http://www.medworm.com/index.php?rid=1418220&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451587%26dopt%3DAbstract Problems and recent advances in measurement of (1-->3)-beta-D-glucan. Nippon Ishinkin Gakkai Zasshi. 2008;49(2):75-80 Authors: Yoshida K, Shoji H, Niki Y Measurement of (1-->3)-beta-D-glucan for invasive fungal infection is used practically in Japan. The problem of false positive results due to the frequent occurrence of non-specific reaction in alkaline treatment, chromogenic automated kinetic assay to measure (1-->3)-beta-D-glucan had been recognized in Japan. But this important problem was resolved in July 2005 by improvement made in the pretreatment reagent in a (1-->3)-beta-D-glucan measurement kit. In this manuscript, we describe the process of improvement of this kit and its clinical usefulness. PMID: 18451587 [PubMed - in process] (Source: Nippon Ishinki... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418220 Sat, 03 May 2008 19:36:33 +0100 1418220 http://www.medworm.com/index.php?rid=1418219&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451588%26dopt%3DAbstract Authors: Kitami Y, Kagawa S, Iijima M A 72 year-old man was referred to our department with white curd-like material on the surface of his tongue as well as the mucosal surface of the lower lip, after unsuccessful treatment with itraconazole for 3 weeks. He also had a history of depression and had received topical steroid and/or antibiotics treatment for persistent oral aphtha and irritation of his upper lip for 4 years. A diagnosis of oral candidiasis was made through positive KOH direct microscopic examination and he was instructed to rinse his oral mucosal lesion with amphotericin B syrup. Although no significant eruption was observed on his upper lip at his first visit, he applied the steroid ointment for 4 weeks and came back to our clinic with his upper lip red and swollen. It wa... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418219 Sat, 03 May 2008 19:36:33 +0100 1418219 http://www.medworm.com/index.php?rid=1418218&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451589%26dopt%3DAbstract Authors: Okumura Y, Ogawa K, Uchiya K, Komori Y, Nonogaki T, Nikai T The biological properties of elastase inhibitor from Aspergillus flavus (AFLEI) were investigated. AFLEI was produced at the highest rate when casamino acid was used as the nitrogen source. When a mixture of AFLEI (approx. molecular weight, 7,500) and elastase from A. flavus (approx. molecular weight, 40,000) was detected using anti-AFLEI antibody, molecular weight of the detected mixture was approximately 48,000, indicating that AFLEI and elastase bound at a proportion of 1 : 1. When immunocompromised mice administrered of immunosuppressive (cyclophosphamide) were infected by inhalation of A. flavus and administered amphotericin B (AMB) alone or in combination with AFLEI, survival rate tended to be higher with combin... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418218 Sat, 03 May 2008 19:36:33 +0100 1418218 http://www.medworm.com/index.php?rid=1418217&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451590%26dopt%3DAbstract Authors: Garcia Marques S, Pedroso E Silva Cde M, Aparecida Resende M, Moura Silva AA, Mendes Caldas Ade J, Lopes Costa JM An experimental study was conducted between January 2002 and April 2003 for the detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin) in skin tests. A total of 194 subjects were attended by spontaneous demand at the Infectious and Parasitic Diseases outpatient clinic of the Federal University of Maranhão-UFMA and at the Department of Microbiology, Federal University of Minas Gerais-UFMG and classified into three groups: patients with chromoblastomycosis caused by F. pedrosoi (n=20), healthy subjects (n=86) and patients with other diseases (n=88). For the skin test, 0.1 ml of the antigen was applied to the anterior side of ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418217 Sat, 03 May 2008 19:36:33 +0100 1418217 http://www.medworm.com/index.php?rid=1418216&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451591%26dopt%3DAbstract Authors: Kawasaki M, Anzawa K, Takeda K, Tanabe H, Mochizuki T, Ishizaki H The genotypes and phenotypes of 77 isolates derived from ascospores produced from two genetically different Arthroderma benhamiae were studied. Specifically, mitochondrial DNA (mtDNA) type, nuclear DNA (nDNA) type, mating type, colony texture, growth rate, urease activity, red pigmentation and hair perforation were examined. The nDNA types based on the restriction fragment length polymorphism (RFLP) in the internal transcribed spacer (ITS) region of ribosomal RNA (rRNA) genes and mating types were inherited from parents independently. mtDNA type was inherited from only one parent. All the phenotypes, except hair perforation and mating type, showed great variations. Those seemed not to be a conclusive factor for ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418216 Sat, 03 May 2008 19:36:33 +0100 1418216 http://www.medworm.com/index.php?rid=1418215&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451592%26dopt%3DAbstract Authors: Abe M, Ogawa Z, Tanuma H, Yamazaki T, Kume H As there is not yet a standardized in vitro susceptibility test of micafungin (MCFG), we evaluated the methods of such testing, focusing on the judgment method of MIC, based on the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2, M38-A, the proposed standards of The Japanese Society for Medical Mycology (JSMM) for yeast (JSMM-Y) and for filamentous fungi (JSMM-F) against Candida spp. and Aspergillus spp. The judgment of MIC value was performed spectrophotometrically and visually in both (NCCLS and JSMM) assays. Only the spectrophotometric MIC judgment against Aspergillus spp. in the NCCLS assay used two end points: 80% inhibitory concentration (IC80) of the growth control and 50% inhibitory concentration (IC50). ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418215 Sat, 03 May 2008 19:36:33 +0100 1418215 http://www.medworm.com/index.php?rid=1418214&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451593%26dopt%3DAbstract We report a case of cutaneous P. boydii infection of the left knee in a 79-year-old Japanese man who was receiving oral predonisolone (25 mg/day) for radiation pneumonitis after radiation therapy on left breast cancer. He presented with a 2-week-history of a lesion on the left knee. A biopsy specimen from the skin lesion revealed granulomatous inflammation with hyphae. Culture of the pus from the skin specimen confirmed the diagnosis of cutaneous P. boydii infection. rDNA ITS sequence was analyzed to confirm the mycological diagnosis. The patient was treated orally with 200 mg/day of itraconazole. The lesion was gradually cured and left a hypertrophic scar. Cutaneous injury may be responsible for an incidence of localized infection. Such rare fungus infection in immunocompromised patients ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418214 Sat, 03 May 2008 19:36:33 +0100 1418214 http://www.medworm.com/index.php?rid=1418213&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451594%26dopt%3DAbstract Authors: Akram Randhawa M Dimethyl sulfoxide (DMSO) is commonly used as a solvent for antifungal drugs. Earlier the author has reported the inhibitory effect of DMSO on the growth of many strains of dermatophytes' colonies in dermasel agar and proposed that this could cause the variations between results of different studies for the evaluation of the activities of antifungal drugs. In studies regarding the determination of the effect of antifungal drugs on the germination of arthrospores of dermatophytes it was observed that relatively higher concentrations of DMSO were being used as a solvent for the antifungal drugs, the final concentration in the media being 5%. Therefore, the present study was aimed at determining the effect of different concentrations of DMSO (1.25 to 10%) on the ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418213 Sat, 03 May 2008 19:36:33 +0100 1418213 http://www.medworm.com/index.php?rid=1418212&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451595%26dopt%3DAbstract Authors: Nakayama H, Aoyama T PMID: 18451595 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418212 Sat, 03 May 2008 19:36:33 +0100 1418212 http://www.medworm.com/index.php?rid=1418211&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18451596%26dopt%3DAbstract Authors: Tokimatsu I PMID: 18451596 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1418211 Sat, 03 May 2008 19:36:33 +0100 1418211 http://www.medworm.com/index.php?rid=1364482&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277046%26dopt%3DAbstract Authors: Mohri S, Watanabe S, Toshio K, Shibuya K, Nishiyama Y, Abe M, Uno J, Oguri T, Maeasaki S, Ikeda R, Abe S After the rapid progress in therapeutic pharmaceutics against onychomycosis caused by dermatophytes in the 1990s, an optimal therapeutic strategy for individual patients with the onychomycosis has become possible for clinical dermatologists. In this review, we discuss on clinical problems concerning this disease and propose recommendable treatments for each patient with topical and/or systemic use of antifungal agents. Finally, with consideration of already published therapeutic guidelines, we stress the necessity of "order-made" therapy for each patient with his/her medical status and wishes taking into account. PMID: 18277046 [PubMed - in process] (Source: Nippon Ishi... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1364482 Fri, 11 Apr 2008 07:14:20 +0100 1364482 http://www.medworm.com/index.php?rid=1239958&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277046%26dopt%3DAbstract Authors: Mohri S, Watanabe S, Toshio K, Shibuya K, Nishiyama Y, Abe M, Uno J, Oguri T, Maeasaki S, Ikeda R, Abe S After the rapid progress in therapeutic pharmaceutics against onychomycosis caused by dermatophytes in the 1990s, an optimal therapeutic strategy for individual patients with the onychomycosis has become possible for clinical dermatologists. In this review, we discuss on clinical problems concerning this disease and propose recommendable treatments for each patient with topical and/or systemic use of antifungal agents. Finally, with consideration of already published therapeutic guidelines, we stress the necessity of "order-made" therapy for each patient with his/her medical status and wishes taking into account. PMID: 18277046 [PubMed - in process] (Source: Nippon Ishi... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1239958 Tue, 19 Feb 2008 08:21:39 +0100 1239958 http://www.medworm.com/index.php?rid=1236662&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277046%26dopt%3DAbstract Authors: Mohri S, Watanabe S, Toshio K, Shibuya K, Nishiyama Y, Abe M, Uno J, Oguri T, Maeasaki S, Ikeda R, Abe S After the rapid progress in therapeutic pharmaceutics against onychomycosis caused by dermatophytes in the 1990s, an optimal therapeutic strategy for individual patients with the onychomycosis has become possible for clinical dermatologists. In this review, we discuss on clinical problems concerning this disease and propose recommendable treatments for each patient with topical and/or systemic use of antifungal agents. Finally, with consideration of already published therapeutic guidelines, we stress the necessity of "order-made" therapy for each patient with his/her medical status and wishes taking into account. PMID: 18277046 [PubMed - as supplied by publisher] (Sourc... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1236662 Sat, 16 Feb 2008 20:20:08 +0100 1236662 http://www.medworm.com/index.php?rid=1236661&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277047%26dopt%3DAbstract Authors: Mori T This paper reports on medical history from the end of the Edo period to the present and development of studies on infectious diseases, especially medical mycology including systemic fungal diseases. With the inflow of Dutch studies at the end of the Edo period and the adoption of European, mainly German, medicine in the Meiji Restoration, Japanese medical studies gradually developed. However, evolution in the medical field as well as other scientific fields was prevented during the 2nd World War. After the War, there was marked progress in scientific fields and medical research made strong advances. In the past 20 years, basic fungal studies and clinical fungal diseases, especially clinical analysis, clinical diagnosis and treatment of systemic fungal infections have pr... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1236661 Sat, 16 Feb 2008 20:20:08 +0100 1236661 http://www.medworm.com/index.php?rid=1236660&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277048%26dopt%3DAbstract Conclusion: The treatment protocol seems promising, but poor compliance is a problem with the oral treatment regimens. The shampoo therapy is only partially effective, with 35% of subjects remaining positive for T. tonsurans after this therapy. In order to eradicate this disease, we have renewed the guidelines for T. tonsurans infection. PMID: 18277048 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1236660 Sat, 16 Feb 2008 20:20:08 +0100 1236660 http://www.medworm.com/index.php?rid=1236659&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277049%26dopt%3DAbstract Authors: Shibata A, Kaneko T, Makimura K, Onozaki M, Ogihara T, Shibata H, Kikuchi K, Abe S Ability for growth support and species differentiation by colony features were compared on two commercial chromogenic agars, CHROMagar Candida and newly developed Pourmedia Vi Candida. Eleven strains (ten species) of standard strains and twenty-four isolates (five species) of clinical strains were tested. All isolates were grown on both agar plates in 48 hours at 35 degrees C, however, several species had not matured in 22 hours. Color of the colonies for each strain were stable on both agars. The results show that Pourmedia Vi Candida is equivalent to CHROMagar Candida in its ability to differentiate species as a primary culture plate. PMID: 18277049 [PubMed - as supplied by publisher] (Sou... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1236659 Sat, 16 Feb 2008 20:20:08 +0100 1236659 http://www.medworm.com/index.php?rid=1236658&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277050%26dopt%3DAbstract Authors: Kang Y, Iida S, Yamamoto S, Kogure T, Tanaka R, Mikami Y Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1236658 Sat, 16 Feb 2008 20:20:08 +0100 1236658 http://www.medworm.com/index.php?rid=1121540&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18161220%26dopt%3DAbstract Authors: Sugita T PMID: 18161220 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1121540 Sun, 30 Dec 2007 01:09:19 +0100 1121540 http://www.medworm.com/index.php?rid=1121539&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18161221%26dopt%3DAbstract Authors: Maesaki S PMID: 18161221 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1121539 Sun, 30 Dec 2007 01:09:19 +0100 1121539 http://www.medworm.com/index.php?rid=1121538&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18161222%26dopt%3DAbstract Authors: Shibuya K PMID: 18161222 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1121538 Sun, 30 Dec 2007 01:09:19 +0100 1121538 http://www.medworm.com/index.php?rid=1121537&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18161223%26dopt%3DAbstract Authors: Mikami Y PMID: 18161223 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1121537 Sun, 30 Dec 2007 01:09:19 +0100 1121537 http://www.medworm.com/index.php?rid=1121536&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18161224%26dopt%3DAbstract Authors: Naka W PMID: 18161224 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1121536 Sun, 30 Dec 2007 01:09:19 +0100 1121536 http://www.medworm.com/index.php?rid=1063993&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18046851%26dopt%3DAbstract [Abstracts of the 51st Annual Meeting of the Japanese Society for Medical Mycology, November 9-10, 2007, Gifu, Japan] Nippon Ishinkin Gakkai Zasshi. 2007;48 Suppl 1:40-93 Authors: PMID: 18046851 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1063993 Sun, 02 Dec 2007 01:04:59 +0100 1063993 http://www.medworm.com/index.php?rid=1044943&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975530%26dopt%3DAbstract Authors: Shibaki H Over the 2 year period from October, 1997 to September, 2005, the clinical efficacy of 125 mg/day of terbinafine was evaluated in 356 patients with onychomycosis. Of these, 253 patients were followed up for 6 months after oral treatment of terbinafine, 120 for 1 year, and 56 for 2 years. The improvement ratio increased depending on follow-up period: 30.4% in 6 months, 65.0% in 1 year, and 67.9% in 2 years. However, in 25 patients who showed regression from onychomycosis at the 1 year period, 8 patients (32.0%) relapsed. The muddy rate of the first toenail was decreased from pre-treatment with terbinafine in 92.1% at 6 months, 91.7% at 1 year and 87.5% at 2 years. It is considered that efficacy of this medication is maintained within 1 year after the treatment, but th... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1044943 Thu, 22 Nov 2007 14:28:34 +0100 1044943 http://www.medworm.com/index.php?rid=1044942&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975534%26dopt%3DAbstract Authors: Sei Y, Sugita T, Maesaki S, Shibuya K, Mikami Y, Naka W PMID: 17975534 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1044942 Thu, 22 Nov 2007 14:28:34 +0100 1044942 http://www.medworm.com/index.php?rid=1002090&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975529%26dopt%3DAbstract Authors: Yamaguchi M, Ohkusu M, Biswas SK, Kawamoto S Cryptococcus neoformans is an opportunistic human pathogen belonging to basidiomycetous fungi and has unique properties in cell cycle progression. The purpose of this study was to measure the duration of the cell cycle in this yeast. Under standard liquid culture conditions (1% yeast extract, 1% polypeptone, and 1% glucose; 24 degrees C; and 150 rpm), the doubling time of exponentially growing C. neoformans was 132 +/- 16 min (mean +/- standard deviation), and the durations of the G1, S, G2, and M phases were about 71, 18, 25, and 18 min, respectively. DNA synthesis started before bud emergence, and finished by the time the size of the bud became 1/4 that of the mother cell. The doubling time of the daughter cells was about twice th... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002090 Tue, 30 Oct 2007 04:00:00 +0100 1002090 http://www.medworm.com/index.php?rid=1002089&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975530%26dopt%3DAbstract Authors: Shibaki H Over the 2 year period from October, 1997 to September, 2005, the clinical efficacy of 125 mg/day of terbinafine was evaluated in 356 patients with onychomycosis. Of these, 253 patients were followed up for 6 months after oral treatment of terbinafine, 120 for 1 year, and 56 for 2 years. The improvement ratio increased depending on follow-up period: 30.4% in 6 months, 65.0% in 1 year, and 67.9% in 2 years. However, in 25 patients who showed regression from onychomycosis at the 1 year period, 8 patients (32.0%) relapsed. The muddy rate of the first toenail was decreased from pre-treatment with terbinafine in 92.1% at 6 months, 91.7% at 1year and 87.5% at 2 years. It is considered that efficacy of this medication is maintained within 1 year after the treatment, but the... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002089 Tue, 30 Oct 2007 04:00:00 +0100 1002089 http://www.medworm.com/index.php?rid=1002088&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975531%26dopt%3DAbstract Authors: Cho T, Aoyama T, Toyoda M, Nakayama H, Chibana H, Kaminishi H Quorum sensing through farnesol, a quorum sensing molecule, regulates virulence and morphogenesis in Candida albicans. Farnesol and high cell density of C. albicans repress hyphal formation in a minimal medium containing N-acetyl-D-glucosamine. Global transcription profiling at an early stage of quorum sensing by C. albicans in the N-acetyl-D-glucosamine medium was analyzed. Twenty-two of a total of 53 genes responded to both farnesol and high cell density. From in silico analysis and previous published data, nine of these genes including those encoding amino acid biosynthesis were controlled by the Gcn4p regulator. Nine other genes which included genes encoding central carbon metabolism were controlled by negative ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002088 Tue, 30 Oct 2007 04:00:00 +0100 1002088 http://www.medworm.com/index.php?rid=1002087&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975532%26dopt%3DAbstract Authors: Kajikazawa T, Sugita T, Takashima M, Nishikawa A Yeasts of 17 processed fresh edible (raw) sea urchins obtained from seven countries were analyzed. In total, 45 to 7 x 10(4) colony-forming units (CFU)/g of sea urchins were recovered, and 23 yeast species were identified. Of these species, six pathogenic yeasts (Candida albicans, C. sake, Debaryomyces hansenii, Pichia anomala, Rhodotorula mucilaginosa, and Trichosporon mucoides) were detected from 11 sea urchins (65%). As these yeasts are opportunistic pathogens, infections in healthy individuals normally will not occur, but it should be understood that processed fresh edible sea urchin includes such opportunistic yeast pathogens. PMID: 17975532 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002087 Tue, 30 Oct 2007 04:00:00 +0100 1002087 http://www.medworm.com/index.php?rid=1002086&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975533%26dopt%3DAbstract Authors: Hasegawa T, Gonoi T, Ito J, Kogure T, Yazawa K, Mikami Y A PCR primer specific to Nocardia farcinica was prepared based on sequence information of random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that are frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL). PMID: 17975533 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002086 Tue, 30 Oct 2007 04:00:00 +0100 1002086 http://www.medworm.com/index.php?rid=1002085&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17975534%26dopt%3DAbstract Authors: Sei Y, Sugita T, Maesaki S, Shibuya K, Mikami Y, Naka W PMID: 17975534 [PubMed - as supplied by publisher] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=1002085 Tue, 30 Oct 2007 04:00:00 +0100 1002085 http://www.medworm.com/index.php?rid=873539&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17667895%26dopt%3DAbstract Authors: Hiruma S PMID: 17667895 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873539 Sat, 15 Sep 2007 13:01:33 +0100 873539 http://www.medworm.com/index.php?rid=873538&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17853533%26dopt%3DAbstract Authors: Nishimoto K PMID: 17853533 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873538 Sat, 15 Sep 2007 13:01:33 +0100 873538 http://www.medworm.com/index.php?rid=873537&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17853534%26dopt%3DAbstract Authors: Kato T PMID: 17853534 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873537 Sat, 15 Sep 2007 13:01:33 +0100 873537 http://www.medworm.com/index.php?rid=873536&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17853536%26dopt%3DAbstract Authors: Nishikawa A PMID: 17853536 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873536 Sat, 15 Sep 2007 13:01:33 +0100 873536 http://www.medworm.com/index.php?rid=873535&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17853537%26dopt%3DAbstract Authors: Mochizuki T PMID: 17853537 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873535 Sat, 15 Sep 2007 13:01:33 +0100 873535 http://www.medworm.com/index.php?rid=873534&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17853538%26dopt%3DAbstract Authors: Fujihiro M PMID: 17853538 [PubMed - indexed for MEDLINE] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=873534 Sat, 15 Sep 2007 13:01:33 +0100 873534 http://www.medworm.com/index.php?rid=817322&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17667895%26dopt%3DAbstract Authors: PMID: 17667895 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=817322 Thu, 23 Aug 2007 13:36:52 +0100 817322 http://www.medworm.com/index.php?rid=775103&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17667894%26dopt%3DAbstract Authors: Khan S, Singhal S, Mathur T, Upadhyay DJ, Rattan A Disulfiram, an alcohol antagonistic drug has been on the market since 1949 with 80% bioavailability and an established safety profile. Recently it has been reported as a P-glycoprotein efflux pump modulator. Herein we report its antifungal potential. The MIC50 and MIC90 of disulfiram for yeast isolates is 4 and 8 micro g/ml, respectively, and the MIC range is 1-16 micro g/ml for both fluconazole sensitive and resistant strains. Interestingly, disulfiram also showed fungicidal activity on Aspergillus spp. with MIC50 and MIC90 of 2 and 8 micro g/ml, respectively. PMID: 17667894 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=775103 Fri, 03 Aug 2007 00:05:54 +0100 775103 http://www.medworm.com/index.php?rid=775102&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17667895%26dopt%3DAbstract Authors: Harada T, Hiruma M, Nishimoto K, Katoh T, Nishikawa A, Mochizuki T, Fujihiro M PMID: 17667895 [PubMed - in process] (Source: Nippon Ishinkin Gakk...) Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=775102 Fri, 03 Aug 2007 00:05:54 +0100 775102 http://www.medworm.com/index.php?rid=643433&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502841%26dopt%3DAbstract Authors: Kageyama A, Mikami Y Following recent advances in medical technology, the increased number of immunocompromised patients such as those with organ transplants has led to an increase in opportunistic infections due to Nocardia. Although nocardiosis has been considered to be rare, recent reports indicate that the incidence of the infection is increasing. The Nocardia asteroides group is the principal source of infectious species, but the definition of species in Nocardia is problematic because species identification of N. asteroides has been based on mainly morphological and biochemical properties. Additionally, it was assumed that a clinical strain with properties that did not fully coincide with existing species was N. asteroides sensu lato. This caused problems in both clinica... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=643433 Wed, 30 May 2007 17:05:20 +0100 643433 http://www.medworm.com/index.php?rid=643432&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502842%26dopt%3DAbstract Authors: Shinoda H, Nishimoto K This paper is a clinical study of 57 cases of infection with Trichophyton tonsurans (T. tonsurans) examined in our clinic between January 2004 and July 2006. The patients were 31 high school students, 19 junior high school students, 2 primary school students, 1 kindergartener, and 4 sports instructors. The male:female ratio was 51:6. Most patients were male Judo practitioners. Patients were clinically categorized as follows: 13 cases of tinea capitis {10 containing black dot ringworms (BDR), 2 scaled, and 1 with inflammation}, 41 cases of tinea corporis, 1 case of tinea manum, and 7 carriers. Five patients displayed both tinea capitis and tinea corporis. Among tinea corporis patients, 21 displayed annular erythemas, whereas 19 displayed small circular ey... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=643432 Wed, 30 May 2007 17:05:20 +0100 643432 http://www.medworm.com/index.php?rid=643431&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502844%26dopt%3DAbstract We report a case of black dot ringworm on the right forearm caused by Trichophyton (T.) tonsurans. A 16-year-old male high school Judo-wrestler visited our clinic on September 30, 2005, complaining of a round erythematosquamous eruption with a distinct margin on the right forearm. Black dots were observed in the lesion. KOH-Parker ink prepared direct microscopy revealed abundant large endotrix arthroconidia in the hair shaft. Culture yielded yellowish-brown colonies. The isolate produced numerous round, short club-shaped microconidia along the hyphae unstained with lactophenol cotton blue (resembling matchsticks) and chlamydospores. PCR-RFLP analysis of internal transcribed spacer regions of ribosomal DNA revealed a banding pattern compatible with T. tonsurans. The lesion was cured by dail... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=643431 Wed, 30 May 2007 17:05:20 +0100 643431 http://www.medworm.com/index.php?rid=643430&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502845%26dopt%3DAbstract Authors: Yaguchi T, Takizawa K, Taguchi H, Tanaka R, Kubota T, Kubota Y, Kubota M, Fukushima K Silver has long been known to have an antimicrobial activity against bacteria and other microorganisms, and has been used as eating utensils, as dental fillings and so on. We developed a novel adduct, GX-95, of silver with nanometer-scale particles to peptidic hydrolysates from collagen. Antifungal activity of the adduct against pathogenic yeasts and filamentous fungi was examined in terms of minimal inhibitory concentrations (MICs). GX-95 was found to possess strong and broad antifungal activities against all fungi examined in the following MICs: 0.25 to 0.31 microg/ml for Candida albicans including resistant strains to fluconazole, itraconazole and flucytosine, 0.05 to 0.2 microg/ml for Cry... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=643430 Wed, 30 May 2007 17:05:20 +0100 643430 http://www.medworm.com/index.php?rid=608089&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502841%26dopt%3DAbstract Authors: Kageyama A, Mikami Y Following recent advances in medical technology, the increased number of immunocompromised patients such as those with organ transplants has led to an increase in opportunistic infections due to Nocardia. Although nocardiosis has been considered to be rare, recent reports indicate that the incidence of the infection is increasing. The Nocardia asteroides group is the principal source of infectious species, but the definition of species in Nocardia is problematic because species identification of N. asteroides has been based on mainly morphological and biochemical properties. Additionally, it was assumed that a clinical strain with properties that did not fully coincide with existing species was N. asteroides sensu lato. This caused problems in both clinica... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=608089 Thu, 17 May 2007 23:13:04 +0100 608089 http://www.medworm.com/index.php?rid=608088&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502842%26dopt%3DAbstract Authors: Shinoda H, Nishimoto K This paper is a clinical study of 57 cases of infection with Trichophyton tonsurans (T. tonsurans) examined in our clinic between January 2004 and July 2006. The patients were 31 high school students, 19 junior high school students, 2 primary school students, 1 kindergartener, and 4 sports instructors. The male:female ratio was 51:6. Most patients were male Judo practitioners. Patients were clinically categorized as follows: 13 cases of tinea capitis {10 containing black dot ringworms (BDR), 2 scaled, and 1 with inflammation}, 41 cases of tinea corporis, 1 case of tinea manum, and 7 carriers. Five patients displayed both tinea capitis and tinea corporis. Among tinea corporis patients, 21 displayed annular erythemas, whereas 19 displayed small circular ey... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=608088 Thu, 17 May 2007 23:13:04 +0100 608088 http://www.medworm.com/index.php?rid=608087&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502843%26dopt%3DAbstract We report a case of a 67-year-old woman with chromomycosis on the left upper arm. The plaque was a very small, erythematous and scaly lesion with a diameter of 1.5 cm. Fonsecaea pedrosoi was isolated as the causal fungus, and a number of Phialophora type conidia, the formation of which is considered rare, were observed. Treatment involved surgical excision of the lesion with a 5 mm margin. Follow up three years later revealed no recurrence. In Japan, 536 patients with chromomycosis were reported from 1955 to 2004. This consisted of 296 cases from 1955 to 1981 as reported by Fukushiro, and 240 cases from 1982 to 2004 as reviewed by us. Our examination of data showed that the most common causal fungi was F. pedrosoi with 137 cases (57.15%), followed by Exophiala jeanselmei with a total of 41... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=608087 Thu, 17 May 2007 23:13:04 +0100 608087 http://www.medworm.com/index.php?rid=608086&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502844%26dopt%3DAbstract We report a case of black dot ringworm on the right forearm caused by Trichophyton (T.) tonsurans. A 16-year-old male high school Judo-wrestler visited our clinic on September 30, 2005, complaining of a round erythematosquamous eruption with a distinct margin on the right forearm. Black dots were observed in the lesion. KOH-Parker ink prepared direct microscopy revealed abundant large endotrix arthroconidia in the hair shaft. Culture yielded yellowish-brown colonies. The isolate produced numerous round, short club-shaped microconidia along the hyphae unstained with lactophenol cotton blue (resembling matchsticks) and chlamydospores. PCR-RFLP analysis of internal transcribed spacer regions of ribosomal DNA revealed a banding pattern compatible with T. tonsurans. The lesion was cured by dail... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=608086 Thu, 17 May 2007 23:13:04 +0100 608086 http://www.medworm.com/index.php?rid=608085&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502845%26dopt%3DAbstract Authors: Yaguchi T, Takizawa K, Taguchi H, Tanaka R, Kubota T, Kubota Y, Kubota M, Fukushima K Silver has long been known to have an antimicrobial activity against bacteria and other microorganisms, and has been used as eating utensils, as dental fillings and so on. We developed a novel adduct, GX-95, of silver with nanometer-scale particles to peptidic hydrolysates from collagen. Antifungal activity of the adduct against pathogenic yeasts and filamentous fungi was examined in terms of minimal inhibitory concentrations (MICs). GX-95 was found to possess strong and broad antifungal activities against all fungi examined in the following MICs: 0.25 to 0.31 micro g/ml for Candida albicans including resistant strains to fluconazole, itraconazole and flucytosine, 0.05 to 0.2 micro g/ml for C... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=608085 Thu, 17 May 2007 23:13:04 +0100 608085 http://www.medworm.com/index.php?rid=568697&cid=s_31721_12_f&fid=31721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287718%26dopt%3DAbstract Authors: Okumura Y, Ogawa K, Uchiya K The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid residues and has a calculated molecular weight of 7,526.2. The search for amino acid homology with other proteins demonstrated that amino acid residues 1 to 51 of AFLEI are 100% identical to residues 20 to 70 of the hypothetical protein Afu3g14940. The Michaelis constant (Km) for succinyl L-alanyl- L-alanyl- L-alanyl p-nitroanilide (STANA), and inhibition constant (Ki), for elastase of AFLEI, were found to be 6.7 x 10(2) microM and 4.0 x 10(-2) microM, respectively. Inhibitory ... Nippon Ishinkin Gakk... journals http://www.medworm.com/rss/comments.php?id=568697 Wed, 25 Apr 2007 18:15:02 +0100 568697