MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Oral Microbiology and Immunology' source. Thu, 09 Feb 2012 21:35:09 +0100 http://www.medworm.com/index.php?rid=5674593&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2012.00639.x In this study, the production pathways of the proinflammatory cytokines, tumor necrosis factor‐α (TNF‐α) and interleukin‐1β (IL‐1β), induced by S. mutans in mouse macrophage were examined using a quantitative real‐time polymerase chain reaction and an enzyme‐linked immunosorbent assay. The S. mutans stimulated the expression of TNF‐α and IL‐1β mRNA at a multiplicity of infection of 1 : 100, which increased at 2 and 4 h, respectively, to 24 h. It also induced the production of high levels of the TNF‐α and IL‐1β proteins, which increased at 2 h and reached a peak at 4 and 24 h, respectively. Nuclear factor‐κB (NF‐κB) was activated and reached a maximum level 30 min after the S. mutans treatment. The expression of TNF‐α and IL‐1β mRNA a... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5674593 Wed, 01 Feb 2012 05:00:00 +0100 5674593 http://www.medworm.com/index.php?rid=5644288&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2012.00637.x In this study, we investigated the mechanism of resistance to cationic antimicrobial peptides (AMPs), which are innate immune factors in humans. Because dltA‐D (teichoic acid biosynthesis) was reported to affect the susceptibility to AMPs in other bacterial species, we evaluated the susceptibility of a dltC knockout mutant of S. mutans to the AMPs human beta‐defensin‐1 (hBD1), hBD2, hBD3 and LL37. The dltC mutant exhibited significantly increased susceptibility to AMPs. Regulation of dltC expression involved CiaRH, a two‐component system. Expression of dltC in the wild‐type strain was significantly increased in biofilm cells compared with that in planktonic cells, whereas expression was not increased in a ciaRH knockout mutant. In biofilm cells, we found that susceptibility to L... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5644288 Tue, 31 Jan 2012 20:30:32 +0100 5644288 http://www.medworm.com/index.php?rid=5644289&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2012.00638.x This study suggests that direct infection of ECs by P. gingivalis may worsen atherosclerotic plaque formation via activation of the CATB pathway. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5644289 Sat, 28 Jan 2012 05:00:00 +0100 5644289 http://www.medworm.com/index.php?rid=5534237&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00634.x SummaryStreptococcus mutans is generally recognized as a causative agent of human dental caries. The production of mutacins (bacteriocins) by S. mutans is considered to be an important factor in the colonization and establishment of S. mutans in the dental biofilm. Two types of mutacins have been characterized: the lantibiotics and the non‐lantibiotics. The lantibiotics generally have a wider spectrum of activity than the non‐lantibiotics, which make them attractive targets for development into new antimicrobial modalities. The non‐lantibiotics are much more prevalent among strains of S. mutans and play a significant role in both community‐level and population‐level interactions in the dental biofilm. These interactions are directly mediated through the ComCDE two‐component ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5534237 Fri, 23 Dec 2011 05:00:00 +0100 5534237 http://www.medworm.com/index.php?rid=5534238&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00633.x SummaryStreptococcus gordonii is an important member of the oral biofilm community. As an oral commensal streptococcus, S. gordonii is considered beneficial in promoting biofilm homeostasis. CcpA is known as the central regulator of carbon catabolite repression in Gram‐positive bacteria and is also involved in the control of virulence gene expression. To further establish the role of CcpA as central regulator in S. gordonii, the effect of CcpA on biofilm formation and natural competence of S. gordonii was investigated. These phenotypic traits have been suggested to be important to oral streptococci in coping with environmental stress. Here we demonstrate that a CcpA mutant was severely impaired in its biofilm‐forming ability, showed a defect in extracellular polysaccharide productio... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5534238 Tue, 20 Dec 2011 05:00:00 +0100 5534238 http://www.medworm.com/index.php?rid=5483919&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00631.x SummaryIn periodontitis, an effective host‐response is primarily related to neutrophils loaded with serine proteases, including elastase (NE) and protease 3 (PR3), the extracellular activity of which is tightly controlled by endogenous inhibitors. In vitro these inhibitors are degraded by gingipains, cysteine proteases produced by Porphyromonas gingivalis. The purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (GCF) in relation to periodontal infection. The GCF collected from 31 subjects (nine healthy controls, seven with gingivitis, five with aggressive periodontitis and 10 with chronic periodontitis) was analyzed for the levels of elafin and secretory leukocyte protease inhibitor (SLPI), two main tissue‐derived inhibitors of ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5483919 Wed, 07 Dec 2011 05:00:00 +0100 5483919 http://www.medworm.com/index.php?rid=5542477&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00635.x SummaryThe polysaccharide capsule of Streptococcus pneumoniae is a hallmark for virulence in humans. In its close relative Streptococcus mitis, a common human commensal, analysis of the sequenced genomes of six strains revealed the presence of a putative capsule locus in four of them. We constructed an isogenic S. mitis mutant from the type strain that lacked the 19 open reading frames in the capsule locus (Δcps mutant), using a deletion strategy similar to previous capsule functional studies in S. pneumoniae. Transmission electron microscopy and atomic force microscopy revealed a capsule‐like structure in the S. mitis type strain that was absent or reduced in the Δcps mutant. Since S. mitis are predominant oral colonizers of tooth surfaces, we addressed the relevance of the capsul... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5542477 Thu, 01 Dec 2011 05:00:00 +0100 5542477 http://www.medworm.com/index.php?rid=5534235&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00636.x In this study, we evaluated whether the heat‐shock protein GroEL of Fusobacterium nucleatum, one of the most prevalent bacteria in periodontitis, induces factors that predispose to atherosclerosis in human microvascular endothelial cells (HMEC‐1) and apolipoprotein E‐deficient (ApoE−/−) mice. GroEL induced the expression of chemokines such as monocyte chemoattractant protein‐1 and interleukin‐8 as well as cell adhesion molecules, such as intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and E‐selectin. GroEL induced the activity of tissue factor and reduced the activity of the tissue factor pathway inhibitor. Foam cell formation was induced by GroEL. GroEL‐injected ApoE−/− mice showed significant atherosclerotic lesion progression compared with con... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5534235 Thu, 01 Dec 2011 05:00:00 +0100 5534235 http://www.medworm.com/index.php?rid=5483918&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00632.x In this study, the role of LPS in the secretion of other virulence factors was investigated. The secretion of the epithelial adhesin Aae, the immunoglobulin Fc receptor Omp34 and leukotoxin were examined in a mutant strain with inactivated TDP‐4‐keto‐6‐deoxy‐d‐glucose 3,5‐epimerase (rmlC), which resulted in altered O‐antigen polysaccharides (O‐PS) of LPS. The secretion of Aae and Omp34 was not affected. However, the leukotoxin secretion, which is mediated by the TolC‐dependent type I secretion system, was altered in the rmlC mutant. The amount of secreted leukotoxin in the bacterial growth medium was reduced nine‐fold, with a concurrent four‐fold increase of the membrane‐bound toxin in the mutant compared with the wild‐type strain. The altered leukotoxin secreti... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5483918 Thu, 01 Dec 2011 05:00:00 +0100 5483918 http://www.medworm.com/index.php?rid=5418489&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00629.x SummaryPeriodontal diseases result from the interaction of bacterial pathogens with the host’s gingival tissue. Gingival epithelial cells are constantly challenged by microbial cells and respond by altering their transcription profiles, inducing the production of inflammatory mediators. Different transcription profiles are induced by oral bacteria and little is known about how the gingival epithelium responds after interaction with the periodontopathogenic organism Aggregatibacter actinomycetemcomitans. In the present study, we examined the transcription of genes involved in signaling transduction pathways in gingival epithelial cells exposed to viable A. actinomycetemcomitans. Immortalized gingival epithelial cells (OBA‐9) were infected with A. actinomycetemcomitans JP2 for 24 h a... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5418489 Fri, 18 Nov 2011 04:59:16 +0100 5418489 http://www.medworm.com/index.php?rid=5404951&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00630.x SummaryPeriodontal pathogens, like any other human commensal or pathogenic bacterium, must possess both the ability to acquire the necessary growth factors and the means to adhere to surfaces or reside and survive in their environmental niche. Recent evidence has suggested that sialic acid containing host molecules may provide both of these requirements in vivo for several periodontal pathogens but most notably for the red complex organism Tannerella forsythia. Several other periodontal pathogens also possess sialic acid scavenging enzymes – sialidases, which can also expose adhesive epitopes, but might also act as adhesins in their own right. In addition, recent experimental work coupled with the release of several genome sequences has revealed that periodontal bacteria have a range of ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5404951 Mon, 14 Nov 2011 13:31:29 +0100 5404951 http://www.medworm.com/index.php?rid=5376521&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00628.x SummaryStaphylococcus aureus is responsible for a wide range of different infections ranging in severity from mild to fatal. However, it primarily exists as a commensal organism in a number of different anatomical sites including the nasopharynx. Although colonization itself is a harmless state, colonized individuals are at risk of endogenous infection when S. aureus enters otherwise sterile sites via wounds or indwelling medical devices. As such, studies of colonization may identify important targets for vaccines or other prophylactic approaches. Colonization is a dynamic process; S. aureus must attach to host surfaces, overcome immune components and compete with other commensal microbes. This occurs via a number of surface‐attached and secreted proteins and other factors such as wall... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5376521 Sat, 01 Oct 2011 04:00:00 +0100 5376521 http://www.medworm.com/index.php?rid=5282062&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00627.x SummaryLeptotrichia buccalis ATCC 14201 is a gram‐negative, anaerobic rod‐shaped bacterium resident in oral biofilm at the tooth surface. The sequenced genome of this organism reveals three contiguous genes at loci: Lebu_1525, Lebu_1526 and Lebu_1527. The translation products of these genes exhibit significant homology with phospho‐α‐glucosidase (Pagl), a regulatory protein (GntR) and a phosphoenol pyruvate‐dependent sugar transport protein (EIICB), respectively. In non‐oral bacterial species, these genes comprise the sim operon that facilitates sucrose isomer metabolism. Growth studies showed that L. buccalis fermented a wide variety of carbohydrates, including four of the five isomers of sucrose. Growth on the isomeric disaccharides elicited expression of a 50‐kDa polypep... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5282062 Sat, 01 Oct 2011 04:00:00 +0100 5282062 http://www.medworm.com/index.php?rid=5218176&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00622.x In this study, we used salivary microbiota derived from healthy human subjects to investigate protective effects against colonization and integration of Pseudomonas aeruginosa, an opportunistic bacterial pathogen, into developing or pre‐formed salivary biofilms. When co‐cultivated in saliva medium, P. aeruginosa persisted in the planktonic phase, but failed to integrate into the salivary microbial community during biofilm formation. Furthermore, in saliva medium supplemented with sucrose, the oral microbiota inhibited the growth of P. aeruginosa by producing lactic acid. More interestingly, while pre‐formed salivary biofilms were able to prevent P. aeruginosa colonization, the same biofilms recovered from mild chlorhexidine gluconate treatment displayed a shift in microbial compos... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5218176 Wed, 14 Sep 2011 11:17:59 +0100 5218176 http://www.medworm.com/index.php?rid=5218177&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00624.x In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the pro‐inflammatory cytokines interleukin‐1β, interleukin‐6 and tumor necrosis factor‐α from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs through pathways that involved caspase‐3 but not caspase‐9. Apoptosis was coincident with inhibition of mitogen‐activated protein kinase kinase (MEK) activation. These results show that F. alocis has characteristics in common with established periodontal pathogens and has the potential to contribute to periodontal tissue destruction. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5218177 Tue, 13 Sep 2011 04:00:00 +0100 5218177 http://www.medworm.com/index.php?rid=5204836&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00626.x SummaryPorphyromonas gingivalis is a primary pathogen involved in the initiation and progression of adult chronic periodontitis. Its colonization on oral surfaces is a necessary first step leading to infection. FimA, a subunit protein of major (long) fimbriae, is a well‐known virulence factor. Based on its nucleotide sequence, FimA is classified into several genotypes. We compared here the transcriptional levels of the fimA gene in several P. gingivalis strains using real‐time polymerase chain reaction analysis, fimbrial display on the P. gingivalis surface using transmission electronic microscopy, and the adherence competencies of P. gingivalis strains carrying different types of FimAs towards saliva and Streptococcus gordonii surfaces using mutagenesis analysis. We demonstrated di... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5204836 Sun, 11 Sep 2011 05:09:11 +0100 5204836 http://www.medworm.com/index.php?rid=5204837&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00625.x SummaryTannerella forsythia, a gram‐negative fusiform rod, is implicated in several types of oral anaerobic infections. Most gram‐negative bacteria have OmpA‐like proteins that are homologous to the OmpA protein in Escherichia coli. We identified an OmpA‐like protein in T. forsythia encoded by the tf1331 gene as one of the major proteins by mass spectrometric analysis. Two‐dimensional, diagonal electrophoresis showed that the OmpA‐like protein formed a dimeric or trimeric structure via intermolecular disulfide bonds. A biotin labeling experiment revealed that a portion of the protein was exposed on the cell surface, even though T. forsythia possesses an S‐layer at the outermost cell surface. Using a tf1331‐deletion mutant, we showed that the OmpA‐like protein affected c... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5204837 Thu, 08 Sep 2011 04:00:00 +0100 5204837 http://www.medworm.com/index.php?rid=5130157&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00623.x SummaryThe interaction of microorganisms with fibronectin plays an important role in infective endocarditis. Granulicatella adiacens is a member of the oral microbiota, formerly known as nutritionally variant streptococci, and is often isolated from endocarditis patients. In the present study we identified a surface protein, designated Cha, which binds to fibronectin, by a plaque hybridization procedure using the cshA sequence as probe, which encodes a fibronectin‐binding molecule of Streptococcus gordonii DL1. The cha sequence was highly homologous to cshA and encoded a product of 2351 amino acid residues. The protein comprised a unique sequence in the N‐terminal half region. The C‐terminal region contained nine complete, and one incomplete, 115‐amino acid residue repeat blocks. A... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5130157 Sun, 31 Jul 2011 23:00:00 +0100 5130157 http://www.medworm.com/index.php?rid=5094623&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00620.x SummaryPG27 is required for secretion of virulence factor gingipains, and has recently been proposed as LptO, which is involved in O‐deacylation of lipopolysaccharide. In the present study, a predicted 14 anti‐parallel β‐strand structure of PG27 was ascertained. Deletion study showed that the region from Asp382 to the C‐terminal His391 of PG27 is dispensable for the function of PG27. Analysis of C‐terminal deletion mutants revealed that the region in strand S14 (Asn369–Gly385) is important for activity. Of the gingipain‐defective mutants, ΔThr378–His391 and ΔPhe377–His391 produced amounts of PG27 comparable to those produced by wild‐type cells, suggesting that Thr378–Phe381 contains essential residues for the function of PG27. In contrast, ΔPhe381–His391, ΔAla3... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5094623 Sun, 31 Jul 2011 23:00:00 +0100 5094623 http://www.medworm.com/index.php?rid=5048825&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00619.x This study sought to document the host transcriptome to a P. gingivalis, T. denticola, and T.forsythia challenge as a polymicrobial infection using a murine calvarial model of acute inflammation and bone resorption. Mice were infected with P. gingivalis, T. denticola, and T. forsythia over the calvaria, after which the soft tissues and calvarial bones were excised. A Murine GeneChip® array analysis of transcript profiles showed that 6997 genes were differentially expressed in calvarial bones (P < 0.05) and 1544 genes were differentially transcribed in the inflamed tissues after the polymicrobial infection. Of these genes, 4476 and 1035 genes in the infected bone and tissues were differentially expressed by upregulation. Biological pathways significantly impacted by the polymic... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5048825 Fri, 22 Jul 2011 00:35:22 +0100 5048825 http://www.medworm.com/index.php?rid=5007057&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00618.x SummaryA collection of Streptococcus sanguinis strains from patients with endocarditis (n = 21) and from the oral cavity (n = 34) was subjected to a multi‐locus sequence typing analysis using seven housekeeping genes, carbamoyl‐phosphate synthetase (carB), Co/Zn/Cd efflux system component (czcD), d‐alanyl‐d‐alanine ligase (ddl), DNA polymerase III (dnaX), glucose‐6‐phosphate dehydrogenase (gdh), DNA‐directed RNA polymerase, beta subunit (rpoB) and superoxide dismutase (sodA). The scheme was expanded by the inclusion of two the putative virulence genes, bacitracin‐resistance protein (bacA) and saliva‐binding protein (ssaB), to increase strain discrimination. Extensive intra‐species recombination was apparent in all genes but inter‐species recombination was al... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=5007057 Fri, 08 Jul 2011 14:17:29 +0100 5007057 http://www.medworm.com/index.php?rid=4932857&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00616.x This study aimed to determine levels and proportions of periodontal bacteria in neonatal gastric aspirates obtained from complicated pregnancies and the respective maternal oral and vaginal samples using a quantitative polymerase chain reaction approach, and also to determine the origin of the neonate’s bacteria by sequence comparisons between the three sites. Aggregatibacter actinomycetemcomitans and Tannerella forsythia were not observed in the neonates or in the women’s vaginas. Interestingly, Porphyromonas gingivalis was identified in the neonates in two samples (2.98E+02 and 1.75E+02 cells ml−1) and in association with Fusobacterium nucleatum, which was observed at high prevalence (10%) and at high levels reaching up to 2.32E+03 cells ml−1. Although F. nucleatum was a... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4932857 Tue, 31 May 2011 23:00:00 +0100 4932857 http://www.medworm.com/index.php?rid=4891894&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00617.x SummaryAggregatibacter actinomycetemcomitans, a common inhabitant of the human upper aerodigestive tract, produces a repeat in toxin (RTX), leukotoxin (LtxA). The LtxA is transcribed as a 114‐kDa inactive protoxin with activation being achieved by attachment of short chain fatty acyl groups to internal lysine residues. Methyl esters of LtxA that were isolated from A. actinomycetemcomitans strains JP2 and HK1651 and subjected to gas chromatography/mass spectrometry contained palmitoyl (C16:0, 27–29%) and palmitolyl (C16:1 cis Δ9, 43–44%) fatty acyl groups with smaller quantities of myristic (C14:0, 14%) and stearic (C18:0, 12–14%) fatty acids. Liquid chromatography/mass spectrometry of tryptic peptides from acylated and unacylated recombinant LtxA confirmed that Lys562 and Lys687 ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4891894 Tue, 31 May 2011 23:00:00 +0100 4891894 http://www.medworm.com/index.php?rid=4861670&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00615.x SummaryDNA‐based methodology for the identification and detection of specific bacteria in dental plaque offers advantages over culturing techniques. One drawback of current molecular techniques like real‐time quantitative polymerase chain reaction (RT‐QPCR) is that they are not able to distinguish between live or dead bacteria. To overcome this problem an assay was assessed to discriminate between viable or dead bacteria using DNA intercalating substances, propidium monoazide (PMA) and ethidium monoazide (EMA) in combination with RT‐QPCR. The assay was tested on oral pathogens: Streptococcus mutans, Prevotella intermedia and Aggregatibacter actinomycetemcomitans. To determine the effectiveness of EMA and PMA, different concentrations (from 5 to 100 μg ml−1) of the substances... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4861670 Wed, 25 May 2011 23:48:39 +0100 4861670 http://www.medworm.com/index.php?rid=4794430&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00611.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4794430 Sat, 07 May 2011 09:35:26 +0100 4794430 http://www.medworm.com/index.php?rid=4676200&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00613.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4676200 Sun, 03 Apr 2011 23:00:00 +0100 4676200 http://www.medworm.com/index.php?rid=4774940&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00612.x SummaryChronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing Porphyromonas gingivalis and Treponema denticola. We have investigated the potential synergistic virulence of P. gingivalis and T. denticola using a murine experimental model of periodontitis. An inoculation regime of four intra‐oral doses of 1 × 1010P. gingivalis cells induced significant periodontal bone loss compared with loss in sham‐inoculated mice, whereas doses of 1 × 109 cells or lower did not induce bone loss. Inoculation with T. denticola with up to eight doses of 1 × 1010 cells failed to induce bone loss in this model. However, four doses of a co‐inoculum of a 1 : 1 ra... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4774940 Thu, 31 Mar 2011 23:00:00 +0100 4774940 http://www.medworm.com/index.php?rid=4704009&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00610.x SummaryMany studies to identify surfaces that enhance the incorporation of dental implants into bone and soft‐tissue have been undertaken previously. However, to succeed in the clinical situation, an implant surface must not support development of microbial biofilms with a pathogenic potential. As a first step in investigating this, we used two‐species and three‐species biofilm models with 16S ribosomal RNA fluorescence in situ hybridization and confocal laser scanning microscopy to examine the effect of surface characteristics on biofilm formation by species that can colonize titanium implants in vivo: Streptococcus sanguinis, Actinomyces naeslundii and Lactobacillus salivarius. Surfaces blasted with Al2O3 (Sa = 1.0–2.0 μm) showed a seven‐fold higher bacterial adhesion af... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4704009 Thu, 31 Mar 2011 23:00:00 +0100 4704009 http://www.medworm.com/index.php?rid=4676199&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00614.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4676199 Thu, 31 Mar 2011 23:00:00 +0100 4676199 http://www.medworm.com/index.php?rid=4663475&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00608.x SummaryIntragastric (i.g.) immunization with recombinant chimeric proteins constructed from the saliva‐binding region (SBR) of Streptococcus mutans surface antigen AgI/II and the A2/B subunits of enterobacterial heat‐labile enterotoxins has been successfully used to induce salivary and circulating antibodies against S. mutans that have protective potential against dental caries. To investigate the mode of action of these vaccine constructs, mice were immunized i.g. with chimeric proteins constructed from SBR and cholera toxin (CT) or the type II enterotoxins of Escherichia coli, LT‐IIa and LT‐IIb. Antigen‐presenting cells (APC) in Peyer’s patches (PP) and mesenteric lymph nodes (MLN) were characterized by flow cytometry. Compared with immunization with SBR alone, chimeric prot... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4663475 Thu, 31 Mar 2011 23:00:00 +0100 4663475 http://www.medworm.com/index.php?rid=4641912&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00609.x SummaryPrimary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air–liquid interface in low‐calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. Porphyromonas gingivalis invaded intracellularly and spread from cell to cell; A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement throug... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4641912 Mon, 28 Mar 2011 17:34:10 +0100 4641912 http://www.medworm.com/index.php?rid=4506865&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00605.x SummaryThe ability for DNA mismatch repair, after oxidative stress‐induced DNA damage, is critical for the persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket. Our previous report demonstrated that, in contrast to other organisms, the repair of oxidative stress‐induced DNA damage involving 8‐oxo‐7,8‐dihydroguanine (8‐oxoG) may occur by a yet‐to‐be described mechanism in P. gingivalis. 8‐oxoG does not block DNA replication; rather, it mispairs with adenine, which can be repaired by the MutY glycosylase. To determine the function of the P. gingivalis MutY homologue in DNA repair, it was insertionally inactivated using the ermF‐ermAM antibiotic cassette and used to create a mutY‐deficient mutant (FLL147) by allelic exchange... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4506865 Tue, 01 Feb 2011 00:00:00 +0100 4506865 http://www.medworm.com/index.php?rid=4418665&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00607.x In this study, we hypothesized that unique S. mutans strains, as determined by DNA fingerprinting from sixty 13‐year‐old subjects with or without experience of caries, would have different susceptibilities to α‐defensins‐1–3 (HNP‐1–3), β‐defensins‐2–3 (HBD‐2–3) and LL‐37. The salivary levels of these peptides in subjects were also measured by enzyme‐linked immunosorbent assays. We found that S. mutans strains from children with active caries showed greater resistance to salivary HNP‐1–2, HBD‐2–3 and LL‐37 at varying concentrations than those from caries‐free subjects. In addition, combinations of these peptides increased their antimicrobial activity against S. mutans either additively or synergistically. The salivary levels of these peptides wer... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4418665 Tue, 01 Feb 2011 00:00:00 +0100 4418665 http://www.medworm.com/index.php?rid=4418666&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2011.00606.x SummaryStreptococcus sanguinis is a major component of the oral flora and an important cause of infective endocarditis. Although S. sanguinis is naturally competent, genome sequencing has suggested significant differences in the S. sanguinis competence system relative to those of other streptococci. An S. sanguinis mutant possessing an in‐frame deletion in the comC gene, which encodes competence‐stimulating peptide (CSP), was created. Addition of synthetic CSP induced competence in this strain. Gene expression in this strain was monitored by microarray analysis at multiple time‐points from 2.5 to 30 min after CSP addition, and verified by quantitative reverse transcription–polymerase chain reaction. Over 200 genes were identified whose expression was altered at least two‐fol... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4418666 Sun, 30 Jan 2011 00:00:00 +0100 4418666 http://www.medworm.com/index.php?rid=4411243&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00603.x SummaryApproximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. The aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. Oligonucleotides targeting the 16S ribosomal DNA gene were designed, synthesized and labeled with digoxigenin. These probes were hybridized with the total nucleic acids of pure cultures or subgingival biofilm samples. Target species included cultivated taxa associated with periodontal health and disease, as well as uncultivated species, such as TM7 sp. OT 346, Mitsuokella sp. OT 131 and Desulfobulbus sp. OT 041. Sensitivity and specifici... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4411243 Fri, 28 Jan 2011 23:40:20 +0100 4411243 http://www.medworm.com/index.php?rid=4411244&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00600.x SummaryWe previously reported that Streptococcus cristatus, an oral commensal, was able to downregulate the interleukin‐8 (IL‐8) response to Fusobacterium nucleatum, a putative oral pathogen in oral epithelial cells. The aim of this study was to extend the understanding of how S. cristatus regulates cytokine expression in oral epithelial cells on a broad basis, and investigate whether the modulation of a Toll‐like receptor (TLR) pathway was involved in this process. KB and TERT‐2 cells were co‐cultured with F. nucleatum and S. cristatus, either alone or in combination. Total RNA was extracted and pathway‐specific focused microarrays were used to profile the transcriptional responses of various cytokine genes and those related to TLR‐mediated signal transduction. Reverse tr... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4411244 Thu, 27 Jan 2011 00:00:00 +0100 4411244 http://www.medworm.com/index.php?rid=4370666&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00601.x SummaryStreptococcus mitis is a viridans streptococcus and a normal commensal of the human oropharynx. However, S. mitis can escape from this niche and cause a variety of infectious complications including infective endocarditis, bacteraemia and septicaemia. It uses a variety of strategies to effectively colonize the human oropharynx. These include expression of adhesins, immunoglobulin A proteases and toxins, and modulation of the host immune system. These various colonization factors allow S. mitis to compete for space and nutrients in the face of its more pathogenic oropharyngeal microbial neighbours. However, it is likely that in vulnerable immune‐compromised patients S. mitis will use the same colonization and immune modulation factors as virulence factors promoting its opportuni... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4370666 Wed, 19 Jan 2011 23:40:41 +0100 4370666 http://www.medworm.com/index.php?rid=4330488&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00604.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4330488 Tue, 11 Jan 2011 01:57:51 +0100 4330488 http://www.medworm.com/index.php?rid=4330485&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00602.x SummaryStreptococcus sanguinis is an oral commensal bacterium and endogenous pathogen in the blood, which is generally naturally competent to take up extracellular DNA. Regarded as a stress response, competence development enables S. sanguinis to acquire new genetic material. The sequenced reference strain SK36 encodes and expresses the genes required for competence (com) and uptake of DNA. Isolated from blood cultures of a confirmed case of infective endocarditis, strain 133‐79 encodes all necessary com genes but is not transformable under conditions permissive for competence development in SK36. Using synthetic competence‐stimulating peptides (sCSP) based on sequences of SK36 and 133‐79 comC, both strains developed competence at similar frequencies in cross‐transformation experi... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4330485 Tue, 11 Jan 2011 01:57:49 +0100 4330485 http://www.medworm.com/index.php?rid=4489053&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00599.x SummaryStreptococcus anginosus, an anginosus group bacterium, is frequently isolated from odontogenic abscesses, and is the oral bacterium that is primarily responsible for producing hydrogen sulfide from l‐cysteine through the action of its l‐cysteine desulfhydrase (βC‐S lyase) enzyme. However, the relationship between its production of hydrogen sulfide and abscess formation has not been investigated. To elucidate the etiological role of hydrogen sulfide in abscess formation, we initially measured, using specific primers, expression of the lcd gene, which encodes βC‐S lyase, in the pus of abscesses that formed in BALB/c mice following subcutaneous injection of S. anginosus into the dorsa. Expression of lcd was >15‐fold higher when l‐cysteine was present than when it was ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4489053 Mon, 10 Jan 2011 00:00:00 +0100 4489053 http://www.medworm.com/index.php?rid=4418667&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00602.x SummaryStreptococcus sanguinis is an oral commensal bacterium and endogenous pathogen in the blood, which is generally naturally competent to take up extracellular DNA. Regarded as a stress response, competence development enables S. sanguinis to acquire new genetic material. The sequenced reference strain SK36 encodes and expresses the genes required for competence (com) and uptake of DNA. Isolated from blood cultures of a confirmed case of infective endocarditis, strain 133‐79 encodes all necessary com genes but is not transformable under conditions permissive for competence development in SK36. Using synthetic competence‐stimulating peptides (sCSP) based on sequences of SK36 and 133‐79 comC, both strains developed competence at similar frequencies in cross‐transformation experi... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4418667 Mon, 10 Jan 2011 00:00:00 +0100 4418667 http://www.medworm.com/index.php?rid=4330487&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00594.x In this study, we identified F. nucleatum genes induced in vivo using in‐vivo‐induced antigen technology (IVIAT). Among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis. The clones encoded for 32 different proteins, of which 28 could be assigned to their functions, which were categorized in translation, transcription, transport, energy metabolism, cell envelope, cellular process, fatty acid and phospholipid metabolism, transposition, cofactor biosynthesis, amino acid biosynthesis, and DNA replication. Putative virulence factors detected were ABC transporter, butyrate‐acetoacetate CoA‐transferase, hemin receptor, hemolysin, hemolysin‐related protein, LysR family transcriptional regulator, serine protease, and transpo... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4330487 Mon, 10 Jan 2011 00:00:00 +0100 4330487 http://www.medworm.com/index.php?rid=4330486&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00599.x SummaryStreptococcus anginosus, an anginosus group bacterium, is frequently isolated from odontogenic abscesses, and is the oral bacterium that is primarily responsible for producing hydrogen sulfide from l‐cysteine through the action of its l‐cysteine desulfhydrase (βC‐S lyase) enzyme. However, the relationship between its production of hydrogen sulfide and abscess formation has not been investigated. To elucidate the etiological role of hydrogen sulfide in abscess formation, we initially measured, using specific primers, expression of the lcd gene, which encodes βC‐S lyase, in the pus of abscesses that formed in BALB/c mice following subcutaneous injection of S. anginosus into the dorsa. Expression of lcd was >15‐fold higher when l‐cysteine was present than when it was ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4330486 Mon, 10 Jan 2011 00:00:00 +0100 4330486 http://www.medworm.com/index.php?rid=4224642&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00597.x SummaryOral viridans group streptococci are the major commensal bacteria of the supragingival oral biofilm and have been detected in human atheromatous plaque. Atherosclerosis involves an ongoing inflammatory response, reportedly involving chronic infection caused by multiple pathogens. The aim of this study was to examine the invasion of human aortic endothelial cells (HAECs) by oral viridans group streptococci and the subsequent cytokine production by viable invaded HAECs. The invasion of HAECs by bacteria was examined using antibiotic protection assays and was visualized by confocal scanning laser microscopy. The inhibitory effects of catalase and cytochalasin D on the invasion of HAECs were also examined. The production of cytokines by invaded or infected HAECs was determined using enz... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4224642 Fri, 03 Dec 2010 00:00:00 +0100 4224642 http://www.medworm.com/index.php?rid=4224641&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00598.x This report provides new insight into the novel complement evasion mechanisms of T. denticola. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4224641 Wed, 01 Dec 2010 00:00:00 +0100 4224641 http://www.medworm.com/index.php?rid=4178873&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00596.x SummaryThe ability of Porphyromonas gingivalis to overcome oxidative stress in the inflammatory environment of the periodontal pocket is critical for its survival. We have previously demonstrated that the recA locus, which carries the bacterioferritin co‐migratory protein (bcp) gene and has a unique genetic architecture, plays a role in virulence regulation and oxidative stress resistance in P. gingivalis. To further characterize the bcp gene, which was confirmed to be part of the bcp‐recA‐vimA‐vimE‐vimF operon, we created a P. gingivalis bcp‐defective isogenic mutant (FLL302) by allelic exchange. Compared with the wild‐type, FLL302 had a similar growth rate, black pigmentation, β‐hemolysis and UV sensitivity. Although there was no change in the distribution of gingipain... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4178873 Thu, 18 Nov 2010 19:26:01 +0100 4178873 http://www.medworm.com/index.php?rid=4178877&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00592.x In this study, we examined the host specificity of B. bacteriovorus strain 109J and its ability to prey on oral pathogens associated with periodontitis, including; Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis and Tannerella forsythia. We further demonstrated that B. bacteriovorus 109J has an ability to remove biofilms of Ei. corrodens as well as biofilms composed of A. actinomycetemcomitans. Bdellovibrio bacteriovorus was able to remove A. actinomycetemcomitans biofilms developed on hydroxyapatite surfaces and in the presence of saliva, as well as to detach metabolically inactive biofilms. Experiments aimed at enhancing the biofilm removal aptitude of B. bacteriovorus with the aid of extracellular... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4178877 Thu, 18 Nov 2010 00:00:00 +0100 4178877 http://www.medworm.com/index.php?rid=4178876&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00593.x This study provides information on the microbial context of the JP2 clone activity in a JP2‐susceptible population and suggests that such individuals may develop immunity to AgP. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4178876 Thu, 18 Nov 2010 00:00:00 +0100 4178876 http://www.medworm.com/index.php?rid=4178875&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00581.x In this study, flowcells and confocal laser scanning microscopy were used to further examine the effects of LuxS‐deficiency on biofilm formation. Similar to the wild‐type strain (UA159), a strain deficient in LuxS (TW26D) bound efficiently to the flowcells and formed microcolonies 4 h after inoculation. Unlike UA159, which accumulated and formed compact, evenly distributed biofilms after 28 h, TW26D showed only loose, sporadic, thin biofilms. DNA microarray analysis revealed alterations in transcription of more than 60 genes in TW26D biofilms by at least 1.5‐fold (P < 0.001). Among the upregulated genes were those for sugar‐specific enzymes II of the phosphotransferase (PTS) system and the atp operon, which codes for the proton‐pumping F‐ATPase. Of the downregulated ... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4178875 Thu, 18 Nov 2010 00:00:00 +0100 4178875 http://www.medworm.com/index.php?rid=4178874&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00595.x SummaryRecently, the predation of Bdellovibrio bacteriovorus on a periodontal pathogen has been described. The current study explores the potential antimicrobial activity of a range of predatory bacteria against key periodontal pathogens. A number of representatives from the Bdellovibrio, Bacteriovorax and Peredibacter lineages (called ‘BALOs’) were tested for their activity towards a group of key periodontal pathogens and an optimal multiplicity of infection was established. As the oral cavity contains a wide variety of bacteria that are not preyed upon, it was investigated if they can have an effect on the predation efficiency of BALOs. It was concluded that a number of important variables involved in bacterial predation are found to be compatible with the composition of the oral mic... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4178874 Thu, 18 Nov 2010 00:00:00 +0100 4178874 http://www.medworm.com/index.php?rid=4117665&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00591.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117665 Sat, 30 Oct 2010 01:23:14 +0100 4117665 http://www.medworm.com/index.php?rid=4117664&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00590.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117664 Sat, 30 Oct 2010 01:23:14 +0100 4117664 http://www.medworm.com/index.php?rid=4117663&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00589.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117663 Sat, 30 Oct 2010 01:23:13 +0100 4117663 http://www.medworm.com/index.php?rid=4117662&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00587.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117662 Sat, 30 Oct 2010 01:23:13 +0100 4117662 http://www.medworm.com/index.php?rid=4117661&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00586.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117661 Sat, 30 Oct 2010 01:23:12 +0100 4117661 http://www.medworm.com/index.php?rid=4117660&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00584.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=4117660 Sat, 30 Oct 2010 01:23:12 +0100 4117660 http://www.medworm.com/index.php?rid=3928692&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00588.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928692 Fri, 03 Sep 2010 09:29:27 +0100 3928692 http://www.medworm.com/index.php?rid=3928691&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00585.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928691 Fri, 03 Sep 2010 09:29:27 +0100 3928691 http://www.medworm.com/index.php?rid=3928690&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00570.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928690 Fri, 03 Sep 2010 09:29:26 +0100 3928690 http://www.medworm.com/index.php?rid=3928689&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00580.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928689 Fri, 03 Sep 2010 09:29:26 +0100 3928689 http://www.medworm.com/index.php?rid=3928688&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00583.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928688 Fri, 03 Sep 2010 09:29:25 +0100 3928688 http://www.medworm.com/index.php?rid=3928687&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00582.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3928687 Fri, 03 Sep 2010 09:29:25 +0100 3928687 http://www.medworm.com/index.php?rid=3842870&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00579.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3842870 Sat, 31 Jul 2010 23:00:00 +0100 3842870 http://www.medworm.com/index.php?rid=3842869&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00576.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3842869 Sat, 31 Jul 2010 23:00:00 +0100 3842869 http://www.medworm.com/index.php?rid=3842868&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00575.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3842868 Sat, 31 Jul 2010 23:00:00 +0100 3842868 http://www.medworm.com/index.php?rid=3842867&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00573.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3842867 Sat, 31 Jul 2010 23:00:00 +0100 3842867 http://www.medworm.com/index.php?rid=3842866&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.2041-1014.2010.00578.x Summary (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=3842866 Sat, 31 Jul 2010 23:00:00 +0100 3842866 http://www.medworm.com/index.php?rid=2882653&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00543.x Conclusion: In this study it was confirmed that mPIP plays a role in the aggregation of oral bacteria. The salivary components promoting aggregation of oral bacteria are considered to be part of the oral defense mechanisms so these findings provide insight into a possible function of mPIP in host defense by promoting aggregation of oral bacteria. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882653 Sun, 11 Oct 2009 23:00:00 +0100 2882653 http://www.medworm.com/index.php?rid=2882652&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00542.x Conclusions: This study showed that the prevalence of HCMV and HSV viruses in GCF is higher in patients suffering from periodontitis compared to periodontally healthy subjects, and that the prevalence of HCMV is higher in deep periodontal pockets. It also brought evidences that periodontal therapy may be associated with virus elimination in diseased sites. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882652 Sun, 11 Oct 2009 23:00:00 +0100 2882652 http://www.medworm.com/index.php?rid=2882651&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00534.x Conclusion: The microbiota of persistent apical periodontitis lesions is composed by diverse types of microorganisms with biofilm-forming capacity, including P. acnes, S. epidermidis and F. nucleatum. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882651 Sun, 11 Oct 2009 23:00:00 +0100 2882651 http://www.medworm.com/index.php?rid=2882650&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00547.x Conclusion: Differences in prevalence of the low and highly cytotoxic strains among serotypes reinforce the hypothesis that serotype b and c isolates of A. actinomycetemcomitans are more virulent than serotype a strains. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882650 Sun, 11 Oct 2009 23:00:00 +0100 2882650 http://www.medworm.com/index.php?rid=2882649&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00546.x Conclusion: These results show the enzymatic properties of l-cysteine desulfhydrase from P. intermedia ATCC25611 and also suggest that the Lcs enzyme, which produces hydrogen sulfide from l-cysteine, is closely associated with the pathogenesis of P. intermedia. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882649 Sun, 11 Oct 2009 23:00:00 +0100 2882649 http://www.medworm.com/index.php?rid=2882648&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00545.x Conclusion: The inverse relationship between proinflammatory potential and ability to cause experimental periodontitis may suggest that an aggressive phenotype could provoke a host response that would compromise the persistence of the pathogen. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882648 Sun, 11 Oct 2009 23:00:00 +0100 2882648 http://www.medworm.com/index.php?rid=2882647&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00544.x Conclusion: Monitoring the proportions of P. gingivalis and T. denticola in subgingival plaque has the potential to help identify sites at significant risk for progression of periodontitis, which would assist in the targeted treatment of disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882647 Sun, 11 Oct 2009 23:00:00 +0100 2882647 http://www.medworm.com/index.php?rid=2882646&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00541.x Conclusions: H. pylori detection in the oral cavity is associated to gastroesophageal disease. In addition, it is suggested that all patients presenting gastric symptoms and H. pylori detection in the oral cavity would begin bacterial treatment immediately. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882646 Sun, 11 Oct 2009 23:00:00 +0100 2882646 http://www.medworm.com/index.php?rid=2882645&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00538.x Conclusion: These data identify that SR-A is expressed by macrophages in response to P. gingivalis, and support that this molecule plays a role in TNF-[alpha] production by macrophages to this organism. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882645 Sun, 11 Oct 2009 23:00:00 +0100 2882645 http://www.medworm.com/index.php?rid=2882644&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00536.x Conclusion: Our study demonstrates that Aml has an ability to lyse planktonic and biofilm cells of clinically isolated mutans streptococci in the presence of Triton X-100. These results suggest the possibility of using Aml as an alternative or additional approach for caries prevention. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882644 Sun, 11 Oct 2009 23:00:00 +0100 2882644 http://www.medworm.com/index.php?rid=2882643&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00524.x Conclusion: Possible sources of transmission and oral propagation of groups (clusters) of strains of C. albicans can occur between diabetic and non-diabetic consorts. A conjugal genotypic identity exists in most C. albicans-positive couples, that is, both consorts share identical or highly related strains; however, this identity is not couple-specific as seen by the coexistence of clusters in couples and unrelated consorts. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2882643 Wed, 30 Sep 2009 23:00:00 +0100 2882643 http://www.medworm.com/index.php?rid=2705027&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00540.x Conclusion: This study and previous polymerase chain reaction-based investigations show that cytomegalovirus is a frequent inhabitant of symptomatic periapical lesions. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705027 Sun, 16 Aug 2009 23:00:00 +0100 2705027 http://www.medworm.com/index.php?rid=2705026&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00530.x Conclusion: Our results indicate the possibility of a worldwide prevalence of serotype k strains with properties in common with those of previously reported strains. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705026 Sun, 16 Aug 2009 23:00:00 +0100 2705026 http://www.medworm.com/index.php?rid=2705025&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00521.x Conclusion: S. mutans PAc is involved in human platelet aggregation and may be one of the virulence factors in the pathogenesis of infective endocarditis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705025 Sun, 16 Aug 2009 23:00:00 +0100 2705025 http://www.medworm.com/index.php?rid=2705024&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00519.x Conclusion: The P. gingivalis typing using fimA PCR has limitations in typeability and discriminatory power. A typing technique for P. gingivalis that is easy to perform but that presents adequate typeability and discriminatory power is needed if we want to better understand the epidemiology of periodontal disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705024 Sun, 16 Aug 2009 23:00:00 +0100 2705024 http://www.medworm.com/index.php?rid=2705023&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00539.x Conclusion: Our data provide evidence of a novel, as yet uncultured methanogenic phylotype in association with oral infections, and indicate possible interactions between methanogens and Synergistes spp., the nature of which deserves further investigation. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705023 Sun, 16 Aug 2009 23:00:00 +0100 2705023 http://www.medworm.com/index.php?rid=2705022&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00537.x Conclusion: Lf suppresses initial attachment of S. gordonii and S. gordonii coaggregates by iron sequestration. This may lead to subsequent inhibition of oral biofilm development. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705022 Sun, 16 Aug 2009 23:00:00 +0100 2705022 http://www.medworm.com/index.php?rid=2705021&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00535.x Conclusions: The clinical and histological findings were consistent with the diagnosis of peripheral giant cell granuloma. Cytomegalovirus has the potential to induce multinucleated giant cells, and the possibility that the virus contribute to the development of peripheral giant cell granuloma merits further study. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705021 Sun, 16 Aug 2009 23:00:00 +0100 2705021 http://www.medworm.com/index.php?rid=2705020&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00533.x Conclusion: These results suggested that oral bacteria have the capability to reactivate HIV from latently infected cells, showing a relationship of mature dendritic cells > immature dendritic cells > macrophages [ge] T cells. Expression of various pattern recognition receptors on these various cell types may provide insight into the primary receptors/signaling pathways used for reactivation by the bacteria. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705020 Sun, 16 Aug 2009 23:00:00 +0100 2705020 http://www.medworm.com/index.php?rid=2705019&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00532.x Conclusion: This study showed the ability of P. gingivalis to induce the shedding/ proteolysis of CD46 from the surface of oral epithelial cells. This may render host cells susceptible to the complement system and contribute to tissue damage and the inflammatory process in periodontitis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705019 Sun, 16 Aug 2009 23:00:00 +0100 2705019 http://www.medworm.com/index.php?rid=2705018&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00531.x Conclusion: These data confirm the possibility of applying beneficial bacteria in periodontal treatment. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705018 Sun, 16 Aug 2009 23:00:00 +0100 2705018 http://www.medworm.com/index.php?rid=2705017&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00529.x Conclusion: The present study suggests that [beta]-catenin enhances IL-1[alpha]-induced OPG production in both PDL cells and hGFs, whereas AP-1 suppresses IL-1[alpha]-induced OPG production in PDL cells. Higher expression of c-fos in PDL cells than in hGFs may implicate a role of PDL cells in alveolar bone resorption in periodontitis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705017 Sun, 16 Aug 2009 23:00:00 +0100 2705017 http://www.medworm.com/index.php?rid=2705016&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00526.x Conclusion: These results suggest that PG1318 plays a role in the regulation of mutation frequency in the bacterium. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705016 Sun, 16 Aug 2009 23:00:00 +0100 2705016 http://www.medworm.com/index.php?rid=2705015&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00525.x Conclusion: Overall, no T-RFLP profile representing a specific bacterial consortium could be associated with the manifestation of symptoms of endodontic origin. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705015 Sun, 16 Aug 2009 23:00:00 +0100 2705015 http://www.medworm.com/index.php?rid=2705014&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00523.x Conclusion: The bacterial molecules recognized by caries-free saliva are significant factors for S. mutans caries formation, and their inhibition could be a therapeutic target. In addition, saliva of caries-free subjects includes significant IgA antibody against antigen I/II of S. mutans, indicating a protective mechanism. However, microorganisms may protect themselves from host immune attack by forming biofilms and decreasing expression of antigen I/II. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705014 Sun, 16 Aug 2009 23:00:00 +0100 2705014 http://www.medworm.com/index.php?rid=2705013&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00512.x Conclusions: The genetic variation observed in OSCC compared with that in control cell lines may account for differences in [beta]-defensin expression. These results suggest a putative role for [beta]-defensins in carcinogenesis and indicate that [beta]-defensins may be useful markers of OSCC. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2705013 Fri, 31 Jul 2009 23:00:00 +0100 2705013 http://www.medworm.com/index.php?rid=2506133&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00502.x Conclusion: Triclosan, indomethacin, caprate and laurate can reduce ADS activity in dental plaque. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506133 Thu, 25 Jun 2009 08:57:30 +0100 2506133 http://www.medworm.com/index.php?rid=2506147&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00520.x Conclusions: LC[ndash]MS/MS offers a sensitive analytical platform to study the disease processes in the root canal environment. The array of proteins expressed in endodontic infections reflects the complex microbial presence and highlights the bacterial species involved in the inflammatory process. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506147 Sun, 14 Jun 2009 23:00:00 +0100 2506147 http://www.medworm.com/index.php?rid=2506146&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00514.x Conclusion: Archaea might be correlated with periodontal diseases. The diversity of Archaea associated with periodontitis was limited. Almost all sequenced amplicons fell into the genus Methanobrevibacter of the Euryarcheota phylum. M. oralis-like species was the predominant but non-exclusive archaeon in the subgingival dental plaque of patients with periodontitis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506146 Sun, 14 Jun 2009 23:00:00 +0100 2506146 http://www.medworm.com/index.php?rid=2506145&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00528.x Conclusions: HCMV and EBV are commonly present in the saliva of periodontitis patients. Periodontitis lesions of systemically healthy subjects seem to constitute the main origin of salivary HCMV, but do not comprise the sole source of salivary EBV. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506145 Sun, 14 Jun 2009 23:00:00 +0100 2506145 http://www.medworm.com/index.php?rid=2506144&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00522.x This study examines the effect of chronic hyperglycemia on BM-M[phi] TLR expression and activation, cell signaling, cytokine production, and phagocytic function in the diabetic state, when challenged with the periodontal stimulus Porphyromonas gingivalis lipopolysaccharide (LPS) to further understand how diabetes and associated hyperglycemia may contribute to the increased susceptibility of people with diabetes to periodontitis.Results: When BM-M[phi], obtained from diabetic NOD mice, are stimulated with P. gingivalis LPS under hyperglycemic conditions the following changes occur: reduced messenger RNA expression and cell surface expression of TLR2, reduced messenger RNA expression and protein production of tumor necrosis factor-[alpha], reduced signal transduction, and a reduction in phag... Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506144 Sun, 14 Jun 2009 23:00:00 +0100 2506144 http://www.medworm.com/index.php?rid=2506143&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00518.x Conclusion: This in vitro study found biofilm-dependent expression of antimicrobial peptides and inflammatory mediators in GECs. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506143 Sun, 14 Jun 2009 23:00:00 +0100 2506143 http://www.medworm.com/index.php?rid=2506142&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00517.x Conclusions: These results indicate that physiological and transient acidification is not sufficient to kill bacteria, but it causes a temporary acid-impairment of their growth ability, which may function as an ecological determinant for microbial composition in dental plaque. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506142 Sun, 14 Jun 2009 23:00:00 +0100 2506142 http://www.medworm.com/index.php?rid=2506141&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00516.x Conclusion: HPVs may be involved in the development or progression of not only OSCC but also of potentially malignant oral lesions. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506141 Sun, 14 Jun 2009 23:00:00 +0100 2506141 http://www.medworm.com/index.php?rid=2506140&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00513.x Conclusion: A simple two-step PCR procedure using the five sets of primer pairs developed in the present study is a rapid and reliable method for the identification of the recognized oral Veillonella species. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506140 Sun, 14 Jun 2009 23:00:00 +0100 2506140 http://www.medworm.com/index.php?rid=2506139&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00510.x Conclusion: The results suggest that among the P. intermedia group bacteria, P. intermedia and P. nigrescens type strains can adhere to and invade epithelial cells, the capability of P. intermedia ATCC 25611T being highest in this context. This strain proved to have a special affinity in binding to epithelial cell lamellipodia. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506139 Sun, 14 Jun 2009 23:00:00 +0100 2506139 http://www.medworm.com/index.php?rid=2506138&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00509.x Conclusion: In the oral cavity of relatively young women without periodontitis, P. nigrescens, unlike P. intermedia, is a frequent finding. Conceivably, pregnant women harbor increasing numbers of P. nigrescens associated with pregnancy gingivitis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506138 Sun, 14 Jun 2009 23:00:00 +0100 2506138 http://www.medworm.com/index.php?rid=2506137&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00508.x Conclusions: LPS from two major periodontal pathogens, A. actinomycetemcomitans and P. gingivalis, induced HMGB1 secretion from HGF. Apoptotic and necrotic cell deaths resulted in the enhancement of HMGB1. Our results suggest that HGF can be a source of HMGB1 by both active secretion and passive release, and that HMGB1 from HGF may contribute to periodontal tissue destruction. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506137 Sun, 14 Jun 2009 23:00:00 +0100 2506137 http://www.medworm.com/index.php?rid=2506136&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00507.x Conclusions: A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression in gingival epithelial cells is mediated by PAR-2. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506136 Sun, 14 Jun 2009 23:00:00 +0100 2506136 http://www.medworm.com/index.php?rid=2506135&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00506.x Conclusion: This is the first report of lysogenic bacteria and their inducible viruses in infected root canals. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506135 Sun, 14 Jun 2009 23:00:00 +0100 2506135 http://www.medworm.com/index.php?rid=2506134&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00503.x Conclusion: These results proved that peptidoglycan biosynthesis plays an important part in a series of bacterial morphologies. The glmM gene may have a constructive role in the virulence properties of S. mutans. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2506134 Sun, 14 Jun 2009 23:00:00 +0100 2506134 http://www.medworm.com/index.php?rid=2341204&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00492.x Conclusion: Conservative dental procedures are a significant cause of bacteraemia. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341204 Sat, 18 Apr 2009 19:43:47 +0100 2341204 http://www.medworm.com/index.php?rid=2341218&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00527.x (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341218 Tue, 14 Apr 2009 04:00:00 +0100 2341218 http://www.medworm.com/index.php?rid=2341217&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00511.x Conclusion: Our results suggest that FimA variations have effects on the structures of biofilms formed by P. gingivalis, which may be an important factor in the pathogenesis of periodontitis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341217 Tue, 14 Apr 2009 04:00:00 +0100 2341217 http://www.medworm.com/index.php?rid=2341216&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00491.x Conclusion: The oral cavity may not be a reservoir for H. pylori in patients with epigastric pain syndrome, the bacterium being detected exclusively in the stomach. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341216 Tue, 14 Apr 2009 04:00:00 +0100 2341216 http://www.medworm.com/index.php?rid=2341215&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00505.x Conclusions: Increased oral Candida infection and carriage titers were found in the transplant population. Although the majority of transplant patients were colonized by C. albicans, C. glabrata appears to emerge as the second most prevalent species. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341215 Tue, 14 Apr 2009 04:00:00 +0100 2341215 http://www.medworm.com/index.php?rid=2341214&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2009.00504.x Conclusion: Infection by HCMV or EBV inhibits the functional ability of macrophages to respond to bacterial challenge, thereby suggesting their pathogenic role in the development of periodontal disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341214 Tue, 14 Apr 2009 04:00:00 +0100 2341214 http://www.medworm.com/index.php?rid=2341213&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00501.x Conclusion: The results demonstrated that A. actinomycetemcomitans had GGT and CGase activities and that the GGT was characterized. The possible role of A. actinomycetemcomitans in glutathione metabolism and H2S production from oral bacteria was discussed. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341213 Tue, 14 Apr 2009 04:00:00 +0100 2341213 http://www.medworm.com/index.php?rid=2341212&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00500.x Conclusion: Collectively, these data suggest that the product used for specimen collection can affect retrieval of antibodies and potentially confound patient diagnosis. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341212 Tue, 14 Apr 2009 04:00:00 +0100 2341212 http://www.medworm.com/index.php?rid=2341211&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00499.x Conclusion: We conclude that the dblB gene among the four GbpC homologous protein genes is most responsible for aggregation in strain 6715. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341211 Tue, 14 Apr 2009 04:00:00 +0100 2341211 http://www.medworm.com/index.php?rid=2341210&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00498.x Conclusion: The present results showed significant variations in the adhesion capacity of the Lactobacillus strains studied. Adhesion to oral surfaces is of primary importance for bacterial colonization in the mouth. Only one of the L. delbrueckii subsp. bulgaricus dairy starter culture strains investigated had a high adhesion percentage. This strain might then be considered for further investigations in the oral environment. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341210 Tue, 14 Apr 2009 04:00:00 +0100 2341210 http://www.medworm.com/index.php?rid=2341209&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00497.x Conclusion: These findings suggest that orf1 and orf2 are associated with positive regulation of the gtfB and gtfC genes. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341209 Tue, 14 Apr 2009 04:00:00 +0100 2341209 http://www.medworm.com/index.php?rid=2341208&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00496.x Conclusion: Repeated oral administration of P. gingivalis induced systemic NO and NOx production in mice, probably by activating iNOS as suggested by the response to 1400W. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341208 Tue, 14 Apr 2009 04:00:00 +0100 2341208 http://www.medworm.com/index.php?rid=2341207&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00495.x Conclusion: This set of very close geographically and temporally collected S. mutans isolates had a degree of gene content variation as high as a previously examined global set of strains. Comparing the frequency of these variable genes, the majority of which have unknown function, among strains of different origins (i.e. different caries status) could help to determine their relevance in S. mutans cariogenicity. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341207 Tue, 14 Apr 2009 04:00:00 +0100 2341207 http://www.medworm.com/index.php?rid=2341206&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00494.x Conclusion: This study suggests that B-lymphocyte involvement in the immune response to A. actinomycetemcomitans through upregulation of RANKL expression potentially contribute to bone resorption in periodontal disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341206 Tue, 14 Apr 2009 04:00:00 +0100 2341206 http://www.medworm.com/index.php?rid=2341205&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00493.x Conclusions: The results showed a pattern of colonization in children similar to that previously found in adults. Differences in the profile between age groups suggest a gradual maturation of the oral microbiota, with it being made up of an increasing number of Orange and Red complex species. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2341205 Tue, 14 Apr 2009 04:00:00 +0100 2341205 http://www.medworm.com/index.php?rid=2179857&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00477.x Conclusion: This study supports the theory that increased caries risk is associated with reduced alkali-generating capacity of the bacteria colonizing the oral cavity; providing compelling evidence to further our understanding of oral alkali-generation in health and disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179857 Fri, 13 Feb 2009 08:58:02 +0100 2179857 http://www.medworm.com/index.php?rid=2179869&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00485.x Conclusions: We implicated two rr genes in the acid tolerance of S. mutans. In particular, smtcs05 is a novel tcs, the sole rr of which is involved in the acid tolerance of S. mutans. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179869 Thu, 12 Feb 2009 05:00:00 +0100 2179869 http://www.medworm.com/index.php?rid=2179868&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00464.x Conclusion: CSA-13 displays broad-spectrum activity against cariogenic and periodontopathic bacteria. CSA-13 was effective against protease-positive Porphyromonas. It was shown to bind to erythrocytes coated with lipopolysaccharide and lipoteichoic acid from diverse bacterial strains. These results suggest that CSA-13 may be useful for the prevention and treatment of oral microbial diseases. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179868 Thu, 12 Feb 2009 05:00:00 +0100 2179868 http://www.medworm.com/index.php?rid=2179867&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00490.x Conclusion: The positioning of lysine is important for binding between Ssp peptide and SRCRP2, and the inhibiting effect on S. mutans binding to the tooth surface. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179867 Thu, 12 Feb 2009 05:00:00 +0100 2179867 http://www.medworm.com/index.php?rid=2179866&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00489.x Conclusion: It is considered that regulation of CSP by glrA in S. mutans and CSP inactivation by S. salivarius are important functions for cell-to-cell communication between biofilm bacteria and oral streptococci such as S. salivarius. Our results provide useful information for understanding the ecosystem of oral streptococcal biofilms, as well as the competition between and coexistence of multiple species in the oral cavity. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179866 Thu, 12 Feb 2009 05:00:00 +0100 2179866 http://www.medworm.com/index.php?rid=2179865&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00488.x Conclusion: The induction of TACE by T cells in response to P. gingivalis may in turn favour the shedding of host cell-bound cytokines into the local microenvironment, potentially amplifying the inflammatory response. In the present experimental system, P. gingivalis cysteine proteases are involved in TACE release by T cells. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179865 Thu, 12 Feb 2009 05:00:00 +0100 2179865 http://www.medworm.com/index.php?rid=2179864&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00487.x Discussion: Our findings highlight a potential role for S. mutans in the pathogenesis of certain cardiovascular diseases. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179864 Thu, 12 Feb 2009 05:00:00 +0100 2179864 http://www.medworm.com/index.php?rid=2179863&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00486.x Conclusions: The fluorescence in situ hybridization technique is a specific and reliable method by which to visually identify the target organisms. The oligonucleotide probes designed in this study will be useful for detecting P. gingivalis and A. actinomycetemcomitans populations. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179863 Thu, 12 Feb 2009 05:00:00 +0100 2179863 http://www.medworm.com/index.php?rid=2179862&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00484.x Conclusions: GAgP subjects presented diminished phagocytic activity of peripheral PMNs and high prevalence and levels of classical periodontal pathogens. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179862 Thu, 12 Feb 2009 05:00:00 +0100 2179862 http://www.medworm.com/index.php?rid=2179861&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00483.x Conclusions: Cdt, a recently identified A. actinomycetemcomitans virulence factor, is capable of inducing a neutralizing antibody response indicating that the toxin is produced during natural infection of humans. The failure of a vast majority (20 of 23) of the LAP subjects to mount a significant anti-Cdt response may in part explain their relative susceptibility to the disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179861 Thu, 12 Feb 2009 05:00:00 +0100 2179861 http://www.medworm.com/index.php?rid=2179860&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00481.x Conclusion: Immunosuppression and antiviral prophylaxis may interact to influence the spectrum of oral HHV-8 strains and the extent of post-transplantation HHV-8 shedding into the mouth. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179860 Thu, 12 Feb 2009 05:00:00 +0100 2179860 http://www.medworm.com/index.php?rid=2179859&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00480.x Conclusion: These results suggest that RecA has a relationship with biofilm formation. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179859 Thu, 12 Feb 2009 05:00:00 +0100 2179859 http://www.medworm.com/index.php?rid=2179858&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00478.x Conclusion: Oral commensal and pathogenic bacteria do not differ qualitatively in how they program DCs. DC-derived MCP-1 induced in response to oral commensal bacteria may play a role, at least in part, in the maintenance of oral tissue integrity by attracting monocytes. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2179858 Thu, 12 Feb 2009 05:00:00 +0100 2179858 http://www.medworm.com/index.php?rid=2030734&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00463.x Conclusion: These results provided strong evidence for the presence of Th17 cells in the sites of chronic inflammation in human periodontal disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030734 Sat, 13 Dec 2008 03:47:59 +0100 2030734 http://www.medworm.com/index.php?rid=2030748&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00462.x Conclusion: These data suggest that iNOS-derived NO is not required for resistance to oral candidiasis in vivo, and that bone marrow-derived macrophages may have iNOS-independent means of generating reactive nitrogen species. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030748 Fri, 12 Dec 2008 05:00:00 +0100 2030748 http://www.medworm.com/index.php?rid=2030747&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00459.x Griswold AR, Nascimento MM, Burne RA. Distribution, regulation and role of the agmatine deiminase system in mutans streptococci. Oral Microbiol Immunol 2009: 24: 79[ndash]82.© 2009 The Authors. Journal compilation. © 2009 Blackwell Munksgaard. The agmatine deiminase system (AgDS) was identified in seven strains of mutans streptococci. Genes encoding the AgDS of Streptococcus rattus FA-1 were sequenced and found to share homology with the agu genes of Streptococcus mutans UA159. With the exception of Streptococcus sobrinus, the AgDS of mutans streptococci appear to be sensitive to carbohydrate catabolite repression. Agmatine inhibited bacterial growth, suggesting that the AgDS degrades a deleterious substance into useful compounds. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030747 Fri, 12 Dec 2008 05:00:00 +0100 2030747 http://www.medworm.com/index.php?rid=2030746&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00447.x Discussion: Multiplex PCR was rapid and effective in the identification of Candida species and allowed the detection of more than one species in the same sample. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030746 Fri, 12 Dec 2008 05:00:00 +0100 2030746 http://www.medworm.com/index.php?rid=2030745&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00482.x Conclusion: The study demonstrates that the combined use of FISH and stereology is a relevant and reliable tool for obtaining unbiased information about the numerical contributions of specific bacterial populations during early biofilm formation. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030745 Fri, 12 Dec 2008 05:00:00 +0100 2030745 http://www.medworm.com/index.php?rid=2030744&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00479.x Conclusion: Our results suggest that specific oral bacterial species, such as S. mutans and A. actinomycetemcomitans, are related to bacteremia and may be etiologic factors for the development of cardiovascular diseases. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030744 Fri, 12 Dec 2008 05:00:00 +0100 2030744 http://www.medworm.com/index.php?rid=2030743&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00476.x Conclusions: Our findings suggest that zinc and copper ions markedly enhanced the adhesion and accumulation of salivary and serum proteins on cells of P. gingivalis and inhibited the coaggregation and hemagglutination of P. gingivalis. These cations might be useful for limiting the settlement of P. gingivalis in the gingival sulcus with the goal of preventing periodontal disease. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030743 Fri, 12 Dec 2008 05:00:00 +0100 2030743 http://www.medworm.com/index.php?rid=2030742&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00475.x Conclusion: These results therefore suggest that A. actinomycetemcomitans lipopolysaccharide may induce the production of NO by HOS cells via a CD14[ndash]TLR4 molecule complex, a cAMP[ndash]PKA pathway, as well as by a PTK, PKC, PLA2, and lipoxygenase-dependent mechanism. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030742 Fri, 12 Dec 2008 05:00:00 +0100 2030742 http://www.medworm.com/index.php?rid=2030741&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00474.x Conclusion: Our results suggest that species specified by molecular methods may have disseminated incidentally into the bloodstream, so interpretation of such results should be carefully undertaken in clinical situations. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030741 Fri, 12 Dec 2008 05:00:00 +0100 2030741 http://www.medworm.com/index.php?rid=2030740&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00473.x Conclusions: These findings show how pathogen-associated molecular patterns, shared by many different periodontopathogenic bacteria, stimulate the resident gingival epithelial cells to inflammatory responses in a TLR-dependent manner. This stimulation may be particularly strong in periodontitis and when T helper type 17 cells provide T-cell help in intercellular cooperation. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030740 Fri, 12 Dec 2008 05:00:00 +0100 2030740 http://www.medworm.com/index.php?rid=2030739&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00470.x Conclusion: Bifids may be routinely isolated from root caries lesions using appropriate cultural methods. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030739 Fri, 12 Dec 2008 05:00:00 +0100 2030739 http://www.medworm.com/index.php?rid=2030738&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00469.x Conclusion: Our results showed a predominance of osteoclast activity in granulomas that was correlated with the Th1 response. The concomitant expression of Treg cell markers suggests a possible suppression of the Th1 response in granulomas. On the other hand, in cysts the Th2 activity is augmented. The mechanisms of periradicular lesion development are still not fully understood but the imbalance of immune and osteoclastic cell activity in cysts and granulomas seems to be critically regulated by Treg cells. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030738 Fri, 12 Dec 2008 05:00:00 +0100 2030738 http://www.medworm.com/index.php?rid=2030737&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00468.x Conclusions: These findings indicate that saliva represents an important initial barrier to IAV infection and underline the complexity of host defense activity of oral secretions. Of interest, antiviral activity of saliva against IAV and HIV differs in terms of specific glandular secretions and proteins that are inhibitory. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030737 Fri, 12 Dec 2008 05:00:00 +0100 2030737 http://www.medworm.com/index.php?rid=2030736&cid=s_25320_77_f&fid=25320&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-302X.2008.00467.x Conclusion: We conclude that P. gingivalis, through lysine gingipain, can subvert the protective host proinflammatory response by direct cytokine degradation. Changes in the crevicular cytokine profile have consequences in periodontal disease pathogenesis that should be considered in the development of diagnostic and therapeutic modalities. (Source: Oral Microbiology and Immunology) Oral Microbiology and Immunology journals http://www.medworm.com/rss/comments.php?id=2030736 Fri, 12 Dec 2008 05:00:00 +0100 2030736