MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Physiological chemistry and physics and medical NMR' source. Sat, 10 Oct 2009 19:36:07 +0100 http://www.medworm.com/index.php?rid=2328879&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256352%26dopt%3DAbstract Authors: Ling G Among the most promising scientific achievements of the 19th century was the recognition that the laws governing the dead world also govern the world of the living and that life has a physical basis called protoplasm. Regrettably, the definition of protoplasm provided then was (inescapably) incorrect, offering a (legitimate) reason for rejecting the concept of protoplasm by an overwhelming majority of later investigators, teachers and other opinion-makers. Without a recognized physical basis, Life itself also faded into the limbo of the unexplainable. However, eventually the needed relevant parts of physics and chemistry to give a more cogent definition of protoplasm became available. That then made possible the construction in the early 1960's of a unifying theory of t... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328879 Mon, 01 Jan 2007 05:00:00 +0100 2328879 http://www.medworm.com/index.php?rid=2328878&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256353%26dopt%3DAbstract Authors: Zorbas YG, Kakuris KK, Deogenov VA, Yerullis KB To demonstrate the effect of sodium supplementation and hypokinesia (HK; diminished movement) on the total bodily sodium (Na+) loss and tissue Na+ deficiency, tissue Na+ content, plasma Na+ level and Na+ loss were measured. Studies were conducted on male Wistar rats during a pre-experimental and experimental period. Animals were equally divided into four groups: unsupplemented vivarium control rats (UVCR), unsupplemented hypokinetic rats (UHKR), supplemented vivarium control rats (SVCR) and supplemented hypokinetic rats (SHKR). A daily supplementation of 3.50 mEq sodium chloride (NaCl) was given to animals in the SVCR and SHKR groups. Gastrocnemius muscle and right femur bone Na+ level decreased (p<0.05), and plasma Na+ level ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328878 Mon, 01 Jan 2007 05:00:00 +0100 2328878 http://www.medworm.com/index.php?rid=2328877&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256354%26dopt%3DAbstract Authors: Jikumaru M, Hiramoto K, Honma T, Sato EF, Sekiyama A, Inoue M We previously observed that female mice survived significantly longer than male mice under fasting conditions. To elucidate the mechanism underlying the sex different effect of fasting, we analyzed various events induced in male and female mice. Kinetic analysis revealed that fasting elicited hypothermia and decreased muscle weight more apparently in male than in female mice. The life-time of male was increased by administration of estradiol while that of female was decreased by ovariectomy. Although plasma levels of estradiol were below detectable levels in male mice, those in female were significantly high and remained unchanged for a fairly long time. Generation of ketone bodies was enhanced more markedly in fema... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328877 Mon, 01 Jan 2007 05:00:00 +0100 2328877 http://www.medworm.com/index.php?rid=2328876&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256355%26dopt%3DAbstract Authors: Aikoh H, Yamato M, Shibahara T Aluminum ion was administered orally to mice for a long period (80 weeks). Aluminum, magnesium, and calcium in the urine were measured weekly, and aluminum in organs, i.e., brain, kidney, liver, heart, lung, and bone, was measured in the final stage. Calcium in the bones was also measured. The results of the urine tests suggest that aluminum ion is hardly absorbed by mice if it is dosed alone. Aluminum ion administered with glycine or glutamic acid is accumulated in vivo, but the presence of tryptophane has little influence on aluminum incorporation. When aluminum remains in the body, the calcium and magnesium excretions increase. The long-term administration of aluminum resulted in the accumulation of aluminum in all organs. The amount of alumin... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328876 Mon, 01 Jan 2007 05:00:00 +0100 2328876 http://www.medworm.com/index.php?rid=2328875&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256356%26dopt%3DAbstract Authors: Ishay JS, Plotkin M, Volynchik S, Bergman DJ In social hornets of sub-family Vespinae there are stripes or areas in the cuticle which are endowed with a bright color (yellow, orange, green) that is different from the customary native brown color. In these brightly colored areas, the middle and bottom layers of the cuticle contain a yellow-colored filling material instead of the ordinary lamellae of which the cuticle is comprised. At high magnification, most of the yellow matter is seen to be made up of yellow granules interlinked by string-like threads. Apart from yellow granules, hemolymph and a surrounding cuticle, these areas are also found to contain glucose, iron and triglycerides as well as enzymes like GOT, GPT, CPK and LDH, in high concentrations that vary considerably... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328875 Mon, 01 Jan 2007 05:00:00 +0100 2328875 http://www.medworm.com/index.php?rid=2328874&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19256357%26dopt%3DAbstract Authors: Okun MR, Edelstein LM PMID: 19256357 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=2328874 Mon, 01 Jan 2007 05:00:00 +0100 2328874 http://www.medworm.com/index.php?rid=1616424&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18613639%26dopt%3DAbstract Authors: Ling G The concept that the basic unit of all life, the cell, is a membrane-enclosed soup of (free) water, (free) K+ (and native) proteins is called the membrane theory. A careful examination of past records shows that this theory has no author in the true sense of the word. Rather, it grew mostly out of some mistaken ideas made by Theodor Schwann in his Cell Theory. (This is not to deny that there is a membrane theory with an authentic author but this authored membrane theory came later and is much more narrowly focussed and accordingly can at best be regarded as an offshoot of the broader and older membrane theory without an author.) However, there is no ambiguity on the demise of the membrane theory, which occurred more than 60 years ago, when a flood of converging evidence... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1616424 Mon, 01 Jan 2007 05:00:00 +0100 1616424 http://www.medworm.com/index.php?rid=1616423&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18613640%26dopt%3DAbstract Authors: Okimura Y, Fujita H, Ogino T, Inoue K, Shuin T, Yano H, Yasuda T, Inoue M, Utsumi K, Sasaki J The aim of the present work is to clarify the mechanism(s) that regulates the accumulation of protoporphyrin IX (PpIX) in human histiocytic lymphoma cell line U937 incubated with 5-aminolevulinic acid (ALA). Biosynthesis and accumulation of PpIX in the cells was determined after incubation with 0.1-5 mM ALA using a flow cytometric technique. The synthesized endogenous PpIX was found to localize predominantly in the mitochondrial region of the cells. The ALA-enhanced PpIX synthesis was suppressed by the presence of either beta-alanine, a competitive inhibitor of beta-transporters on cell membranes, or carbonyl cyanide p-trifluoromethoxyphenyl hydrazone, an uncoupler of mitochondrial ox... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1616423 Mon, 01 Jan 2007 05:00:00 +0100 1616423 http://www.medworm.com/index.php?rid=1616422&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18613641%26dopt%3DAbstract Authors: Ishay JS, Plotkin M, Volynchik S, Shaked M, Schuss Z, Bergman DJ Oriental hornet workers, kept in an Artificial Breeding Box (ABB) without a queen, construct within a few days brood combs of hexagonal cells with apertures facing down. These combs possess stems that fasten the former to the roof of the ABB. In an ABB with adult workers (more than 24 h after eclosion), exposed to an AC (50 Hz) magnetic field of a magnitude of B = 50-70 mGauss, the combs and cells are built differently from those of a control ABB, subjected only to the natural terrestrial magnetic field. The effects of the additional magnetic field consist of (a) 35-55% smaller number of cells and fewer eggs in each comb, (b) disrupted symmetry of building, with many deformed and imperfectly hexagonal cells, and ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1616422 Mon, 01 Jan 2007 05:00:00 +0100 1616422 http://www.medworm.com/index.php?rid=1616421&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18613642%26dopt%3DAbstract Authors: Ling G The centerpiece of this document is an unanswered letter of appeal from the author to Professor Roderick MacKinnon of the Rockefeller University dated November 17, 2003. The aim of the appeal is summarized in the title of this communication. In addition to the 2003 letter, there are also two follow-up letters in this communication, each containing a copy of the 2003 letter and each repeating the appeal. The follow-up letters, dated February 22, 2008 and April 2, 2008 respectively, were also unanswered. To make sure that these letters reached their destination, each was certified with delivery time and date affirmed. Thus the February 22 letter was delivered on the February 24 by the US Postal Service. Two copies of the April 2 follow-up letter were sent. The first copy ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1616421 Mon, 01 Jan 2007 05:00:00 +0100 1616421 http://www.medworm.com/index.php?rid=1616420&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18613643%26dopt%3DAbstract Authors: Okun MR, Edelstein LM PMID: 18613643 [PubMed - in process] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1616420 Mon, 01 Jan 2007 05:00:00 +0100 1616420 http://www.medworm.com/index.php?rid=1554568&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18472468%26dopt%3DAbstract The objective of this study was to identify alternation in various genes in liver of mice after administration of low and high doses of APAP. Male C57BL/6J mice received APAP (30 or 300 mg/kg, i.p.). They were sacrificed after 6 hr and 24 hr for assessment of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), total RNA isolation, cDNA microarray analysis and histopathological analysis of liver injury. Low dose of APAP did not cause hepatotoxicity in mice. However, it was toxic at a high dose. Using microarray technology, we selected changed genes more than 1.5 fold. Gene expression changes were recorded even at a low dose treatment with APAP. Six (6) hr after APAP treatment at low dose, 6 genes were up-regulated and 25 genes were down-regulated. However, 24 hr after treat... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1554568 Sun, 01 Jan 2006 05:00:00 +0100 1554568 http://www.medworm.com/index.php?rid=1554567&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18472469%26dopt%3DAbstract Authors: Kataoka T, Aoyama Y, Sakoda A, Nakagawa S, Yamaoka K Exposure to water in hot springs containing thoron is thought to exercise beneficial effects on hypertension and diabetes mellitus. To put to a test this hypothesis we examined the time dependent changes in the levels of lipid peroxide, vasoactive- and diabetes associated substances in human blood in order to throw further light on the possible beneficial influence of thoron and thermal therapy on the mechanism of hypertension and diabetes mellitus. Every 2 days, nasal inhalation of vapor containing thoron was performed for 40 min. Blood samples were collected after each treatment at 1, 2, and 3 weeks after the first treatment. Results show that the treatment decreased the lipid peroxide levels. The finding suggests that the... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1554567 Sun, 01 Jan 2006 05:00:00 +0100 1554567 http://www.medworm.com/index.php?rid=1554566&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18472470%26dopt%3DAbstract This study aims at showing that during hypokinesia (HK) tissue magnesium (Mg2+) content decreases more with higher Mg2+ intake than with lower Mg2+ intake and that Mg2+ loss increases more with higher than lower tissue Mg2+ depletion due to inability of the body to use Mg2+ during HK. Studies were conducted on male Wistar rats during a pre-HK period and a HK period. Rats were equally divided into four groups: unsupplemented vivarium control rats (UVCR), unsupplemented hypokinetic rats (UHKR), supplemented vivarium control rats (SVCR) and supplemented hypokinetic rats (SHKR). SVCR and SHKR consumed 42 mEq Mg2+ per day. The gastrocnemius muscle and right femur bone Mg2+ content decreased significantly, while plasma Mg2+ level and urine and fecal Mg2+ loss increased significantly in SHKR and ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1554566 Sun, 01 Jan 2006 05:00:00 +0100 1554566 http://www.medworm.com/index.php?rid=1554565&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18472471%26dopt%3DAbstract This article describes simple experimental models that could cogently test the theory of this connectedness and its control by drugs and other cardinal adsorbents. PMID: 18472471 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1554565 Sun, 01 Jan 2006 05:00:00 +0100 1554565 http://www.medworm.com/index.php?rid=1554564&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18472472%26dopt%3DAbstract Authors: Bartzatt R This work presents the analysis of pharmacological properties of a homologous set of cephalothin derivatives formed after inserting an aliphatic ester substituent having from one to ten carbon atoms (ie. -CH3 or -CH2CH3) in place of the former carboxyl group (-C(O)OH). These compounds were shown to have significant correlations and associations in their properties after analysis by pattern recognition methods including cluster analysis, detrended correspondence analysis, and K-means cluster analysis. Formula weight of all derivatives is directly correlated and increases with molar volume, parachor, and molar refractivity. Index of refraction decreases as formula weight of derivatives increases. Polar surface area of all derivatives remains constant at 102.02 A2 as f... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=1554564 Sun, 01 Jan 2006 05:00:00 +0100 1554564 http://www.medworm.com/index.php?rid=753790&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17405408%26dopt%3DAbstract Authors: Aoyama S, Okimura Y, Fujita H, Sato EF, Umegaki T, Abe K, Inoue M, Utsumi K, Sasaki J Mitochondria play an important role in apoptosis by generating reactive oxygen species (ROS) and inducing membrane permeability transition (MPT). Recent studies on alpha-lipoic acid (LA) and its reduced form, dihydrolipoic acid, suggest that these agents (LAs) inhibit apoptosis of cells by means of their antioxidant activity. On the other hand, LAs also stimulate Ca2+-dependent mitochondrial MPT and induce apoptosis of certain cells. Thus, the role of LAs in apoptotic cell death remains obscure. We investigated the mechanism of LA-induced MPT of mitochondria. Biochemical analysis revealed, in the presence of Ca2+, inorganic phosphate and succinate, LA induced uncoupling of oxidative phosphory... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753790 Sun, 01 Jan 2006 05:00:00 +0100 753790 http://www.medworm.com/index.php?rid=753789&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17405409%26dopt%3DAbstract In this study, we examined histological changes of lymphatic follicles at 4, 24, or 48 hrs after sham, 0.25, 0.5, or 15 Gy irradiation in the spleens of BALB/c mice, which are sensitive to radiation compared with other strains, and C57BL/6J mice, which are resistant to radiation, using hematoxylin-eosin staining for lymphatic follicles or methylgreen pyronin staining for plasma cells. Results show that the lymphatic follicles in the spleens of the two mouse strains decreased at 24 or 48 hrs after 15 Gy irradiation. The number of plasma cells in the spleens of sham irradiated BALB/c mice was greater than that of sham irradiated C57BL/6J mice. At 4 hrs after 0.25 Gy irradiation, plasma cells increased in the spleens of the two mouse strains. These findings suggest, by histology, that low-dos... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753789 Sun, 01 Jan 2006 05:00:00 +0100 753789 http://www.medworm.com/index.php?rid=753788&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17405410%26dopt%3DAbstract Authors: Ishay JS, Fuksman E, Ermakov NY, Plotkin M Feeding of diazepam to young hornets completely inhibits or delays development of their ovaries for a relatively long period. In control hornets, the ovaries usually develop within a day or two post eclosion and comb building commences on the second day of life. The hornets then oviposit into the comb cells and the deposited ova give rise to larvae. Trials were performed on parallel groups of hornets of various ages. When the sedative diazepam was administered to hornets aged 0-24 hours the ovaries of these young hornets failed to show any development, so that no oocytes ripened and consequently there was no oviposition whatsoever. Neither were any comb cells built or, at best, only a few were built. When the diazepam was administered... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753788 Sun, 01 Jan 2006 05:00:00 +0100 753788 http://www.medworm.com/index.php?rid=753787&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17405411%26dopt%3DAbstract Authors: Plotkin M, Neufeld A, Ermakov NY, Volynchik S, Bergman DJ, Navon G, Ishay JS The head of the Oriental hornet in situ, detached from a live sample was imaged using Magnetic Resonance Imaging (MRI). This non-invasive method enabled us to visualize the three-dimensional structure of the hornet's brain and intracerebral organs, as based on cubic voxels of 23 microm3. From these images, we could identify various cephalic structures in both supra-esophageal and sub-esophageal locations. In the former location, we identified and visualized the ocelli, ommatidia, mushroom body, lobula, medulla and the compound eyes in the protocerebrum, as well as the olfactory lobe and bases of the antenna in the deutocerebrum, while in the sub-esophageal region we visualized organs such as the mouth... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753787 Sun, 01 Jan 2006 05:00:00 +0100 753787 http://www.medworm.com/index.php?rid=753786&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17405412%26dopt%3DAbstract Authors: Ling G Project 2061 was founded by the American Association for the Advancement of Science (AAAS) to improve secondary school science education. An in-depth study of ten 9 to 12th grade biology textbooks led to the verdict that none conveyed "Big Ideas" that would give coherence and meaning to the profusion of lavishly illustrated isolated details. However, neither the Project report itself nor the Benchmark books put out earlier by the Project carries what deserves the designation of "Big Ideas." Worse, in the two earliest-published Benchmark books, the basic unit of all life forms--the living cell--is described as a soup enclosed by a cell membrane, that determines what can enter or leave the cell. This is astonishing since extensive experimental evidence has unequivocally d... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753786 Sun, 01 Jan 2006 05:00:00 +0100 753786 http://www.medworm.com/index.php?rid=753797&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16583992%26dopt%3DAbstract Authors: Ling GN The primary focus of this communication is to present an updated and advanced version of the theory of close-contact association of molecules and ions through the spatial fixation and aggregation of the adsorbing sites. The last sections of the text also review a collection of relevant in vitro and in vivo experimental findings gathering since seventy years ago. Though some of these findings were published before the theory, old and new, they all support the theory that close-contact association with the beta-, and gamma-carboxyl groups of intracellular proteins causes the selective accumulation of potassium ions (K+) in living cells. PMID: 16583992 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753797 Sat, 01 Jan 2005 05:00:00 +0100 753797 http://www.medworm.com/index.php?rid=753796&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16583993%26dopt%3DAbstract Authors: Aikoh H, Nakamura K, Yamato M, Shibahara T The evidence implicating aluminum as a neurotoxin is mounting. Research with animals and humans has linked aluminum with neuro-cognitive dysfunction and, in some cases, death. Although the relationship between aluminum intake and Alzheimer's disease is still unclear, some experts have recently issued a strong warning that human exposure to aluminum should be limited. The results indicate that the amount of aluminum decreased in the urine in mice that were administered glycine or glutamic acid together with aluminum ion. In the mice in which the amounts of aluminum decreased in the urine, the amount of calcium conversely increased. PMID: 16583993 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medica... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753796 Sat, 01 Jan 2005 05:00:00 +0100 753796 http://www.medworm.com/index.php?rid=753795&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16583994%26dopt%3DAbstract Authors: Plotkin M, Ermakov NY, Bergman DJ, Ishay JS Hornet silk is a polymer of amino acids. One of the known properties of polymers is their electrical activity. The present study describes the results of electrical measurements carried out vertically on the silk cap of pupae of the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae). The measurements undertaken were the temperature-dependent electric current, voltage and resistance, all measured within the range of biological temperatures, as well as the capacitance. The temperature-dependent spontaneous current attained values up to 327 nano Amperes (nA) while the maximal voltage reached 347 millivolt (mV). The electrical resistance was low and steady (1-20 mu omega) at temperatures ranging between 19-32 degrees C, but at lowe... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753795 Sat, 01 Jan 2005 05:00:00 +0100 753795 http://www.medworm.com/index.php?rid=753794&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17022371%26dopt%3DAbstract Authors: Czerlinski G, Ypma T There are essentially two alternative mechanisms for the binding of telomerase to telomeres, assuming that a protective component is initially bound to the telomerase binding region on the telomeres. Either the protective (or blocking) agent first dissociates and telomerase binds thereafter, or telomerase binds first and the protective agent then dissociates from the ternary complex. In the limit, this second possibility permits the ternary complex to become a transition complex (creating another possible mechanism). Numerical simulation of both rapid mixing and chemical relaxation is used to study these alternatives. We aim to determine how the mechanisms may be distinguished experimentally and identify an appropriate experimental design. We show that rap... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753794 Sat, 01 Jan 2005 05:00:00 +0100 753794 http://www.medworm.com/index.php?rid=753793&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17022372%26dopt%3DAbstract We examined the effects of post low-dose (0.5 Gy) X-ray irradiation which reduced the oxidative damage under carbon tetrachloride-induced hepatopathy in acatalasemic or normal mice. As a result, the 0.5 Gy irradiation after carbon tetrachloride administration decreased the glutamic oxaloacetic and glutamic pyruvic transaminase activity in the acatalasemic mouse blood to a level similar to that of the acatalasemic mouse blood not treated with carbon tetrachloride; this is in contrast to a high-dose (15 Gy) irradiation. In the same manner, pathological disorder was improved by 0.5 Gy irradiation. The fat degeneration in normal mice was quickly reduced, in contrast to acatalasemic mice. These findings suggest that low-dose irradiation after carbon tetrachloride administration accelerates the ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753793 Sat, 01 Jan 2005 05:00:00 +0100 753793 http://www.medworm.com/index.php?rid=753792&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17022373%26dopt%3DAbstract The objective of this study was to show that hypokinesia (diminished movement) could affect differently water and electrolyte content in muscles having minimum differences in their function and morphology. To this end, we studied water and electrolyte content in skeletal and cardiac muscles, fluid excretion, electrolyte absorption, and electrolyte levels in plasma, urine and feces of rats during prolonged hypokinesia (HK). Studies were conducted on one-hundred-twenty-six 13-weeks old male Wister rats during a pre-hypokinetic period and a hypokinesia period. Animals were equally divided into two groups: vivarium control rats (VCR) and hypokinetic rats (HKR). Hypokinetic animals were kept in small individual cages which restricted their movements in all directions without hindering food and ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753792 Sat, 01 Jan 2005 05:00:00 +0100 753792 http://www.medworm.com/index.php?rid=753791&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17022374%26dopt%3DAbstract Authors: Ling GN, Fu YZ The solvency of solutes of varying molecular size in the intracellular water of freshly-killed Ehrlich carcinoma cells fits the same theoretical curve that describes the solvency of similar solutes in a 36% solution of native bovine hemoglobin--a protein found only in red blood cells and making up 97.3% of the red cell's total intracellular proteins. The merging of the two sets of data confirms the prediction of the AI Hypothesis that key intracellular protein(s) in dying cells undergo(es) a transition from: (1) one in which the polypeptide NHCO groups assume a fully-extended conformation with relatively strong power of polarizing and orienting the bulk-phase water in multilayers; to (2) one in which most of the polypeptide NHCO groups are engaged in alpha-helic... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753791 Sat, 01 Jan 2005 05:00:00 +0100 753791 http://www.medworm.com/index.php?rid=753808&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15789970%26dopt%3DAbstract Authors: Ling G Most living cells contain a large amount of water. To improve our understanding of this fundamental phenomenon of cell physiology, five theories are critically examined in the light of three sets of relevant experimental findings. These findings are: (1) the diversity and specificity of the percentage water content to tissue type; (2) the limitation imposed by the Law of the Conservation of Energy on postulating membrane pumps and (3) the non-extractability of cell water from the open ends of muscle cells whose membrane covering has been surgically removed. Two of the five theories examined are called respectively the accidental theory (Theory I) and the direct water pump-leak theory (Theory III); both are introduced for the first time here as working hypotheses. Three ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753808 Thu, 01 Jan 2004 05:00:00 +0100 753808 http://www.medworm.com/index.php?rid=753807&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15789971%26dopt%3DAbstract Authors: Miyaguchi C, Muranaka S, Kanno T, Fujita H, Akiyama J, Yoshioka T, Yasuda T Oxidative stress-induced apoptotic cell death has been implicated to play a critical role in the mechanism of corpus luteum regression and follicular atresia. Recent studies suggests that reactive oxygen species (ROS) might play important roles in the regulation of luteal function. The present work describes the inhibitory effect of 17beta-estradiol (E2) on ROS-induced mitochondrial membrane permeability transition (MPT) and apoptosis of Chinese hamster ovary (CHO) cells. ROS generated by Fe2+ and H2O2 induced mitochondrial lipid peroxidation, depolarization, activation of caspase-3 and DNA fragmentation in CHO cells by some E2-inhibitable mechanism. E2 suppressed the Fe2+/H2O2-induced lipid peroxidati... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753807 Thu, 01 Jan 2004 05:00:00 +0100 753807 http://www.medworm.com/index.php?rid=753806&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15789972%26dopt%3DAbstract Authors: Bartzatt R Nicotinic acid is shown to be comparable to dihydropyridine in its capacity to facilitate penetration of an attached antibacterial drug through dermal layers. Antibacterial drugs examined with nicotinic acid or dihydropyridine carriers were beta-lactam antibiotics: methicillin, oxacillin, benzylpenicillin, penicillin F, penicillin dihydro F, propicillin, carbenicillin, penicillin K, penicillin X, and ampicillin. An oxymethyl (-O-CH2-) group is inserted as the linker between the antibiotic and the carrier group. Structure Property Correlations and multivariate methods such as regression analysis, cluster analysis, principal component analysis, discriminate analysis, self-organizing tree algorithm, and factor analysis clearly showed that nicotinic acid performs as an ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753806 Thu, 01 Jan 2004 05:00:00 +0100 753806 http://www.medworm.com/index.php?rid=753805&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15789973%26dopt%3DAbstract Authors: Pertsis V, Sverdlov A, Riabinin K, Kozhevnikov M, Ishay JS Our study focused on the thermoelectric properties of hornet cuticle at different body compartments and under varying states of awakeness. We also measured the temperature alteration patterns in various body parts of the hornet. Electric voltage and current were dependent on: a) the state of wakefulness; b) the part of the body. The current was lowest in dead hornet cuticle, somewhat higher in narcotized hornet cuticle, considerably higher in the cuticle of hornets awakening from anesthesia and highest in fully awake hornets. Voltage values were of the same order for dead and narcotized hornets, but considerably higher in unanesthetized awake hornets and highest in the cuticle of hornets awakening from anesthesia. At o... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753805 Thu, 01 Jan 2004 05:00:00 +0100 753805 http://www.medworm.com/index.php?rid=753804&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15789974%26dopt%3DAbstract Authors: Amyan A, Ayrapetyan S The changes of wet and dry weights of barley seed in different periods of swelling were studied in seeds treated with Extremely Low Frequency Electromagnetic Fields (ELF EMF), Static Magnetic Fields (SMF) and Mechanical Vibrations (MV) in cold (4 degrees C) and warm (20 degrees C) distilled water as well as in seeds non-treated (control). The metabolic dependent seed hydration, dry weight loss and water binding in seed were modulated by preliminary EMF, SMF and MV-induced treatment of distilled water. The specific electrical conductivity (SEC) of control and treated distilled water was measured before the seed incubation. Frequency and intensity "windows" (i.e. range of frequency or intensity) for the effect of EMF, MV and SMF (correspondingly) on seed hy... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753804 Thu, 01 Jan 2004 05:00:00 +0100 753804 http://www.medworm.com/index.php?rid=753803&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268120%26dopt%3DAbstract Authors: Bartzatt R, Cirillo SL, Cirillo JD Ampicillin is a beta-lactam antibiotic that is effective against gram-negative bacteria. Ampicillin has a single carboxyl group (-C(O)OH) within its structure which is suitable for forming ester compounds. Diazomethane and diazoethane were utilized to react with ampicillin to form the methyl and ethyl esters, respectively. The ester derivatives of ampicillin were solubilized together (mole ratio 1:1) in LB media and penicillin resistant Escherichia coli added to measure antibacterial activity. Growth inhibition of bacteria was monitored by optical density after a known time period and with known specific concentrations of the ampicillin esters present. Significant growth inhibition of penicillin resistant bacteria occurred at concentrations o... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753803 Thu, 01 Jan 2004 05:00:00 +0100 753803 http://www.medworm.com/index.php?rid=753802&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268121%26dopt%3DAbstract Authors: Yorimitsu M, Muranaka S, Sato EF, Fujita H, Abe K, Yasuda T, Inoue M, Utsumi K We previously showed that Ca2+-induced cyclosporin A-sensitive membrane permeability transition (MPT) of mitochondria occurred with concomitant generation of reactive oxygen species (ROS) and release of cytochrome c (Free Rad. Res.38, 29-35, 2004). To elucidate the role of alpha-tocopherol in MPT, we investigated the effect of alpha-tocopherol on mitochondrial ROS generation, swelling and cytochrome c release induced by Ca2+ or hydroxyl radicals. Biochemical analysis revealed that alpha-tocopherol suppressed Ca2+-induced ROS generation and oxidation of critical thiol groups of mitochondrial adenine nucleotide translocase (ANT) but not swelling and cytochrome c release. Hydroxyl radicals also induced... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753802 Thu, 01 Jan 2004 05:00:00 +0100 753802 http://www.medworm.com/index.php?rid=753801&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268122%26dopt%3DAbstract The objective of this study was to show that prolonged restriction of motor activity (hypokinesia) could reduce phosphate (P) deposition and contribute to P loss with tissue P depletion. To this end, measurements were made of tissue P content, P absorption, plasma P levels, urinary and fecal P excretion of rats during and after hypokinesia (HK) and daily phosphate supplementation. Studies were conducted on male Wistar rats during a pre-hypokinetic period, a hypokinetic period and a post-hypokinetic period. All rats were equally divided into four groups: unsupplemented vivarium control rats (UVCR), unsupplemented hypokinetic rats (UHKR), supplemented vivarium control rats (SVCR) and supplemented hypokinetic rats (SHKR). Bone and muscle P content, plasma intact parathyroid hormone (iPTH) lev... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753801 Thu, 01 Jan 2004 05:00:00 +0100 753801 http://www.medworm.com/index.php?rid=753800&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268123%26dopt%3DAbstract Authors: Galushko DV, Ermakov NY, Bergman DJ, Ishay JS The hornet is an endothermic insect. Daily variations in hornet surface temperature were measured. Three peaks were found between 9:30 and 10: 30 a.m., 11 and 12 a.m. and between 2 and 3 p.m. Electrical current and voltage values were highest along the head. Electrical current along the gaster and the head flowed towards the thorax, i.e., from body parts with minimal temperature towards the body part with maximal temperature. Current and voltage values measured across the cuticle of the gaster were about 5nA and 100 mV, respectively, and these were of the same order of magnitude as the current and voltage values along the cuticle. It was found that: 1) temperature regulation most probably originates in the thorax and 2) there is a ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753800 Thu, 01 Jan 2004 05:00:00 +0100 753800 http://www.medworm.com/index.php?rid=753799&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268124%26dopt%3DAbstract Authors: Galushko DV, Ermakov NY, Bergman DJ, Ishay JS Social insects, belonging to the order Hymenoptera, maintain a fixed, optimal temperature in their nest. Thus, in social wasps and hornets, the optimal nest temperature is 29 degrees C, despite the fact that they are distributed in regions of varying climates both in the northern and southern hemispheres of the globe. Since hornets and bees are relatively small insects, determination of their own body temperature as well as that of their nest and the brood was made via thermometers or by the use of infrared (IR) rays. It has been suggested that thermoregulation in social insect colonies is effected primarily by the adult insects via muscle activation, that is, fluttering of their wings, which can raise both their own and the ambien... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753799 Thu, 01 Jan 2004 05:00:00 +0100 753799 http://www.medworm.com/index.php?rid=753798&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16268125%26dopt%3DAbstract Authors: Ling GN, Hu WX At equilibrium, the concentration ratio of poly(ethylene gycol) (PEG-4000) in a dialysis sac containing a 35.1% solution of native bovine hemoglobin over that in the external solution is 0.196 +/- 0.028 (mean +/- SD). This apparent equilibrium distribution constant or rho-value of 0.196, when viewed side-by-side with the near-equal distribution of sucrose and raffinose in similar native-hemoglobin dominated water suggests all (rather than 80%) of the water in this solution has been altered by the native hemoglobin and is no longer free liquid water. Based on Ling's equation for solute exclusion, we found that an excess of water-to-water interaction energy of a mere 4.25 cal/mole could account for both the observed exclusion of PEG-4000 and non-exclusion of sucro... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753798 Thu, 01 Jan 2004 05:00:00 +0100 753798 http://www.medworm.com/index.php?rid=753818&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139279%26dopt%3DAbstract Authors: Miyamoto M, Sato EF, Nishikawa M, Nishizawa Y, Morii H, Inoue M To understand the role of nitric oxide (NO) in the regulation of cellular metabolism in peritoneal macrophages under physiological low oxygen tension, its effect on the respiration and energy metabolism was examined with casein-induced peritoneal macrophages from the rat. Intraperitoneal injection of casein transiently induced peritoneal infiltration of neutrophils (peaked on day 1) followed by the migration of macrophages that peaked on day 2. Western blotting analysis using antibodies against inducible type of NO synthase (iNOS) revealed that macrophages appeared in the peritoneal cavity during an early stage (approximately day 2) but not during the late stage (day 3 approximately) of inflammation expressed iNOS... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753818 Wed, 01 Jan 2003 05:00:00 +0100 753818 http://www.medworm.com/index.php?rid=753817&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139280%26dopt%3DAbstract Authors: Polewski K An absorption and fluorescence spectral and temporal studies on the solubilzation properties of adrenaline in micellar environment in sodium dodecyl sulfate (SDS) and in tetradodecyltrimethyl ammonium bromide (TTABr) has been carried out. Observed Stokes shifts have been correlated with polarity parameters which allowed an estimate of the dielectric constant of the adrenaline environment in SDS and TTABr micelles at 44 and 58, respectively. Experiments with methanol-water mixtures indicate that the hydrogen bonding formation with solvent and the hydrophilic nature of adrenaline influence its solubilization in micelles. Fluorescence and anisotropy decay analysis has shown that neutral adrenaline in SDS micelle is partitioned between aqueous phase (70%) and less polar... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753817 Wed, 01 Jan 2003 05:00:00 +0100 753817 http://www.medworm.com/index.php?rid=753816&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139281%26dopt%3DAbstract The objective clinical, blinded morphological and immuno-histochemical data from this experiment support the initial conclusion that neuronal connection is necessary for the normal ultraviolet exposure dermal reaction. Based upon this study, we further propose the existence of an exteroceptive sensory system in which melanocytes, with direct nervous system connection initiate an ultraviolet radiation reactive response that mobilizes both conventional endocrine and immune pathways. PMID: 15139281 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753816 Wed, 01 Jan 2003 05:00:00 +0100 753816 http://www.medworm.com/index.php?rid=753815&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139282%26dopt%3DAbstract Authors: de Souza CT, Nunes WM, Gobatto CA, de Mello MA The present study was designed to evaluate the effects of aerobic exercise training on glucose tolerance and insulin secretion of obese male Wistar rats (monosodium glutamate [MSG] administration, 4 mg/g-body weight, each other day, from birth to the 14th day). Fourteen weeks after the drug administration, the rats were separated into two groups: MSG-S (sedentary) and MSG-T (T = swimming, 1 h/day, 5 days/week, with an overload of 5% body weight for 10 weeks). Rats of the same age and strain injected with saline were used as control (C) and subdivided into two groups: C-S and C-T. Insulin and glucose responses during an oral glucose tolerance test (GTT) were evaluated by the estimation of the total areas under serum insulin (AI) an... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753815 Wed, 01 Jan 2003 05:00:00 +0100 753815 http://www.medworm.com/index.php?rid=753814&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139283%26dopt%3DAbstract In this study, MRS technique has been used to clarify the interactions of the dihydrophyridines Nifedipine and Lacidipine within the lipid bilayer. 1D and 2D 1H MRS at high field have been employed to examine the behavior of unilamellar dimyristoyl-phosphatidylcholine liposomes when the two drugs have been inserted in the bilayer. In particular, the study represents an innovative application of 2D 1H NOESY technique to clarify different mechanisms of interactions of small molecules inside model membranes. On the other hand, 31P measurements have been performed in multilamellar dimyristoyl-phosphatidylcholine lipsomes to detect alterations of lipid polymorphic phases. The experiments show that the two dihydropyridines interact with the lipids by different modalities. Lacidipine undergoes a ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753814 Wed, 01 Jan 2003 05:00:00 +0100 753814 http://www.medworm.com/index.php?rid=753813&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15139284%26dopt%3DAbstract Authors: Pertsis V, Sverdlov A, Ishay JS The present study investigated thermoelectric phenomena in the cuticle of the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae). This was done in dependence on the pigment extant at various cuticular region, that is, the brown cuticle in which the primary pigment is melanin and embedded within the cuticle, and the yellow stripes in which the yellow pigment is comprised of purines and pteridines that are located in special pockets between the upper part of the cuticle and the basement membrane. The yellow pigment could be separated from the cuticle proper, but the brown pigment was not thus separable. We found that all cuticular regions of the gaster evinced a thermoelectric response, in that with rise in temperature there was a rise in th... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753813 Wed, 01 Jan 2003 05:00:00 +0100 753813 http://www.medworm.com/index.php?rid=753812&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15552723%26dopt%3DAbstract Authors: Ling GN Over the centuries, a vast amount of evidence has been gathering that layers of water sometimes measuring tens of thousands of water molecules thick exhibit altered properties in consequence of exposure to some solid surfaces. Yet, a clear cut theory based on the laws of physics that would predict this kind of long range dynamic ordering of water molecules has been long missing. It is thus with great joy that I announce that a new theory has been developed, which offers theoretical confirmation of the phenomena of long-range dynamic structuring of water by appropriate solid surfaces and which gives clear cut quantitative answers to some key questions about the phenomenon. Thus, for example, under an ideal condition, an idealized checkerboard of alternatingly positively... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753812 Wed, 01 Jan 2003 05:00:00 +0100 753812 http://www.medworm.com/index.php?rid=753811&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15552724%26dopt%3DAbstract In this study, therefore, we examined the effect of CEP on Ca2+- and Fe2+/ADP-induced MPT of isolated mitochondria. CEP inhibited Ca2+-induced swelling, depolarization, Cyt.c release, and the release of Ca2+ in a concentration dependent manner. CEP also inhibited Ca2+-induced generation of reactive oxygen species and Fe/ADP-induced swelling and lipid peroxidation. Furthermore, CEP suppressed Ca2+-induced thiol modification of adenine nucleotide transloase (ANT). These results suggested that CEP suppressed MPT by a decrease in affinity of cyclophilin D for ANT. From these results it was concluded that the suppression of MPT by CEP might be due to its inhibitory action on Ca2+ release and antioxidant activity and that CEP might suppress the mechanism of apoptotic cell death when directly int... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753811 Wed, 01 Jan 2003 05:00:00 +0100 753811 http://www.medworm.com/index.php?rid=753810&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15552725%26dopt%3DAbstract Authors: Ohno S, Togami I, Sei T, Ida K, Tsunoda M, Yamaoka K, Kanazawa S, Hiraki Y The purpose of this study was to evaluate clinically T1-weighted spin-echo imaging (T1-SE) and T1-weighted opposed-phase gradient-echo (T1-opposed GRE) in medical examinations for vertebral metastases using 0.2 Tesla MRI. Twenty-one patients (9 males and 12 females, 105 vertebrae) with non-neoplastic lesions were assigned to the normal group (Group N), whereas 27 patients (16 males and 11 females, 133 vertebrae) with malignant metastatic vertebral lesions were assigned to the metastatic group (Group M). Using quantitative analysis, the contrast to noise ratio (CNR) for bone marrow to muscle in the region of interest (ROI) defined by the operator were determined with both groups, whereas the CNR for lesi... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753810 Wed, 01 Jan 2003 05:00:00 +0100 753810 http://www.medworm.com/index.php?rid=753809&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15552726%26dopt%3DAbstract Authors: Pertsis V, Sverdlov A, Kozhevnikov M, Ishay JS When an Oriental hornet Vespa orientalis is subjected to ether anesthesia and then exposed to ultraviolet A light (UVAL) (at a wavelength of 366 nm), it commences showing signs of awakening by starting to move its limbs. While in the process of waking the voltage on its body surface surges sharply from 17-180 mV (median = 71.0) to a level of 93-570 mV (median = 327.5). This elevated level is maintained for several minutes but subsequently drops sharply to starting level. The increase in voltage is throughout accompanied by fluttering of the wings and movements of the legs, as well as attempts to extricate itself from the bindings to the electrodes. These movements by the awakening hornet persist for several minutes even after the ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753809 Wed, 01 Jan 2003 05:00:00 +0100 753809 http://www.medworm.com/index.php?rid=753833&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403272%26dopt%3DAbstract Authors: Kanno T, Kobuchi H, Kajitani N, Utsumi T, Yano H, Horton AA, Yasuda T, Utsumi K It has been reported that inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase suppress cell proliferation and induce apoptosis. One inhibitor which induces apoptosis is mevastatin. However, the molecular mechanism of apoptosis induction is not well understood so the effects of mevastatin on various functions of HL-60 cells were investigated. We confirmed that mevastatin activated caspase-3 by release of cytochrome c (Cyt. c) from mitochondria through a membrane permeability transition mechanism and also induced typical fragmentation and ladder formation of DNA in HL-60 cells. These effects were inhibited by mevalonate, a metabolic intermediate of cholesterol biosynthesis. Mevalo... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753833 Tue, 01 Jan 2002 05:00:00 +0100 753833 http://www.medworm.com/index.php?rid=753832&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403273%26dopt%3DAbstract Authors: Czerlinski G, Levin R, Ypma T The kinetics of the reaction of hemoglobin with molecular oxygen, in which rapid mixing is followed by a fast temperature jump, is numerically simulated. We use the system of Ackers (1998) which distinguishes four forms of bi-ligated hemoglobin. The data suggest the involvement of isomerization steps for bi- and triliganded hemoglobin. Our first model assumes a linear addition of oxygen with one path to and from each bi-ligated species. Our second model allows cross-overs between paths, as described by Ackers (1998). Our third model exploits the observation (Perrella et al., 1990) that two of the four bi-ligated forms are at low concentration. We explore whether these models can be distinguished experimentally. We find a narrow oxygen concentratio... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753832 Tue, 01 Jan 2002 05:00:00 +0100 753832 http://www.medworm.com/index.php?rid=753831&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403274%26dopt%3DAbstract Authors: Maity H, Jarori GK Fluorescence quenching studies on the PII isoenzyme of yeast hexokinase have been performed using charged as well as polar uncharged quenchers. In both 'open' (i.e. in the absence of glucose) and 'closed' (i.e. in the presence of glucose) forms of the enzyme, bimolecular quenching rate constant (kq) for acrylamide is significantly larger than that of KI, indicating that all the tryptophans are not fully exposed to the solvent. Overall accessibility of tryptophans towards KI was greater in the presence of glucose than in the absence of glucose. At high ionic strength, the value of bimolecular quenching rate constant (kq) for KI did not change suggesting that the average environment of the accessible tryptophan residue(s) is almost neutral. Quenching by KI is ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753831 Tue, 01 Jan 2002 05:00:00 +0100 753831 http://www.medworm.com/index.php?rid=753830&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403275%26dopt%3DAbstract We report herein that the vespan cornea is densely covered with cupola-shaped protrusions, which in the compound eye have bases about 0.2 microm in diameter and an average height of 0.03 microm while upon the ocelli their diameter is rather variable, with some measuring 0.2-0.3 microm, and a length of submicrons to microns. Interspersed among the brief, cupola-shaped structures there are winding, longer structures whose height appears uniform at around 0.03 microm. It appears that the structures are formed from the epicuticular layer of the cornea and we conjecture that in both the compound eye and in the ocelli they serve to enhance the absorption of light, as well as to reduce light reflection. PMID: 12403275 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753830 Tue, 01 Jan 2002 05:00:00 +0100 753830 http://www.medworm.com/index.php?rid=753829&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403276%26dopt%3DAbstract Authors: Bartzatt R, Koziol K, Benish T, Stoddard J Tetracycline is a widely used broad spectrum antibiotic. A derivative of tetracycline was synthesized by methylation (-CH3) of the phenolic hydroxyl group, with the use of diazomethane (CH2N2). A methyl ether group is then formed from the reaction with diazomethane, which replaces the hydroxyl group. The newly formed derivative has reduced hydrogen bonding capability relative to the unmodified tetracycline. An infrared spectra shows the appearance of the ether group on the derivative and the Log P calculations indicate that the derivative has increased lipophilic tendency. The Lipophilic Substituent Constant calculated for the tetracycline derivative is 0.46, indicating a lipophilic substituent. The tetracycline derivative was soluble... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753829 Tue, 01 Jan 2002 05:00:00 +0100 753829 http://www.medworm.com/index.php?rid=753828&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12403277%26dopt%3DAbstract Authors: Aikoh H, Tagawa T, Shibahara T The extracorporeal excretion of mercury from the organs by [Mo3S4(Hnta)3]2- (referred to as the NTA complex) solution was investigated using mice exposed to metallic mercury vapor. A decrease in mercury levels was seen in the organs of mice that were administered NTA complex solution when compared to organs in mice receiving L-cysteine or water. Moreover, in mice that were administered NTA complex solution, mercury level in the kidneys decreased at the third and fifth days following mercury exposure. These results suggest that NTA complex solution has the effect of releasing mercury in the living-body as seen when mercury levels are compared with those in the organs of mice that were administered L-cysteine or water. PMID: 12403277 [PubMed - ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753828 Tue, 01 Jan 2002 05:00:00 +0100 753828 http://www.medworm.com/index.php?rid=753827&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841327%26dopt%3DAbstract Authors: Kanno T, Fujita H, Muranaka S, Yano H, Utsumi T, Yoshioka T, Inoue M, Utsumi K The opening of mitochondrial membrane permeability transition (MPT) pores, which results in a cyclosporin A (CsA)-sensitive and Ca(2+)-dependent dissipation of the membrane potential (delta psi) and swelling (classical MPT), has been postulated to play an important role in the release of cytochrome c (Cyt.c) and also in apoptotic cell death. Recently, it has been reported that CsA-insensitive or Ca(2+)-independent MPT can be classified as non-classic MPT. Therefore, we studied the effects of apoptosis-inducing agents on mitochondrial functions with respect to their CsA-sensitivity and Ca(2+)-dependency. CsA-sensitive mitochondrial swelling, depolarization, and the release of Ca2+ and Cyt.c were indu... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753827 Tue, 01 Jan 2002 05:00:00 +0100 753827 http://www.medworm.com/index.php?rid=753826&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841328%26dopt%3DAbstract Authors: Bartzatt R, Kasher L A Structure Activity Relationship (SAR) study was accomplished with six aromatic compounds which have a nitrogen mustard (N-mustard) substituent. N-mustard agents are very important for the clinical treatment of many types of cancers. All N-mustard agents synthesized alkylated a nucleophilic primary amine (p-chloroaniline) in aqueous solvent at pH 7.4 and 37 degrees C. Rate constants and rate equations were determined for the alkylation reactions by monitoring the formation of a fluorescent complex formed when fluorescamine complexes the unreacted p-chloroaniline. Fluorescamine complexation of the unreacted primary amine halts the alkylation reaction and allows the determination of remaining unreacted primary amine, which in turn permits the determination ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753826 Tue, 01 Jan 2002 05:00:00 +0100 753826 http://www.medworm.com/index.php?rid=753825&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841329%26dopt%3DAbstract Authors: Yamaoka K, Mori S, Nomura T, Taguchi T, Ito T, Hanamoto K, Kojima S The increase in lipid peroxide levels in mice brain following Fe3+ administration was about 50% of that when 1-methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) was administered. This may be due to excessive oxidation by Fe3+, and was supported by the decrease in activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX), Na+,K(+)-ATPase activity and membrane fluidity after Fe3+ administration. Relatively low-dose X-ray irradiation (0.5 Gy) inhibited lipid peroxidation associated with Fe3+ administration and restored the decreased activities of the above antioxidant enzymes and Na+,K(+)-ATPase, and membrane fluidity to the levels in the non-Fe(3+)-administe... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753825 Tue, 01 Jan 2002 05:00:00 +0100 753825 http://www.medworm.com/index.php?rid=753824&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841330%26dopt%3DAbstract Authors: Yamaoka K, Nomura T, Wang DH, Mori S, Taguchi T, Ishikawa T, Hanamoto K, Kira S The catalase activities in blood and organs of the acatalasemic (C3H/AnLCsbCsb) mouse of the C3H strain are lower than those of the normal (C3H/AnLCsaCsa) mouse. We conducted a study to examine changes in the activities of antioxidant enzymes, such as catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPX), the total gluathione content, and the lipid peroxide level in the brain, which is more sensitive to oxidative stress than other organs, at 3, 6, or 24 hr following X-ray irradiation at doses of 0.25, 0.5, or 5.0 Gy to the acatalasemic and the normal mice. No significant change in the lipid peroxide level in the acatalasemic mouse brain was seen under non-irradiation conditions. How... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753824 Tue, 01 Jan 2002 05:00:00 +0100 753824 http://www.medworm.com/index.php?rid=753823&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841331%26dopt%3DAbstract Authors: Hillman H The aim of biology is the study of the properties of cells in living intact animals. Many steps of research procedures in biochemistry change the entropy, and therefore, the free energy of the chemical reactions from those in vivo. Sections prepared for histology, histochemistry, electron microscopy and immunocytochemistry, yield limited information about the original cells in life, although their problems have been documented. Approaches to the biological and histological difficulties are suggested. PMID: 12841331 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753823 Tue, 01 Jan 2002 05:00:00 +0100 753823 http://www.medworm.com/index.php?rid=753822&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841332%26dopt%3DAbstract Authors: van Dalen JP It is proposed that before light and oxidation of organic compounds became the predominant energy suppliers of the living cell, electron charge energy derived from heat energy by mineral particles, was the first energy source. The basis of this hypothesis is the finding that Al2O3 in electrolytic condensors can produce an electron driving force with a potential high enough for electrolysis of water and subsequent reduction of CO2 into organic molecules. This electron driving force is likely to originate from one-way movement of electrons in tunnel structures of the Al2O3 layer, driven by temperature kinetic energy. PMID: 12841332 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753822 Tue, 01 Jan 2002 05:00:00 +0100 753822 http://www.medworm.com/index.php?rid=753821&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841333%26dopt%3DAbstract Authors: Pertsis V, Nagly L, Kristianpoller N, Ishay JS Effects of short-term UV irradiation were investigated on various cuticular parts of workers and queens of the Oriental hornet, to wit: brown strip, yellow strip and wing. On each preparation of the afore-mentioned, a reading of the relative optical density (ROD) was taken prior to, immediately following, and 15-30 minutes after its irradiation as compared to white light irradiation. The results showed that brief UV irradiation causes changes in the ROD of hornet cuticle, and that these changes in ROD are different in brown than in yellow cuticle. Those in yellow strip are induced by the presence or absence of the active yellow pigment, whose quantity in worker cuticle is different than in queen cuticle, probably due to the variou... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753821 Tue, 01 Jan 2002 05:00:00 +0100 753821 http://www.medworm.com/index.php?rid=753820&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841334%26dopt%3DAbstract This study deals with the effect which solar irradiation of short wavelength, particularly ultraviolet (UV), exerts on the activities of hornets. The findings are based on multi-annual observations carried out during the years 1985, 1989 and 1998 on hornet nests in the field. At the peak of UV radiation, which occurs at noon, hornet activity is greater by 1-2 orders of magnitude than that during the morning or evening hours. The main visible hornet activity appears to be the removal of soil particles from the nest so as to enlarge its volume, enable the building of additional combs and also increase the size of existing combs. Hornet flight during peak insolation hours is characterized by its briefness (5-20 seconds only) and brevity (to distances of 5-10 meters only) as compared to flight... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753820 Tue, 01 Jan 2002 05:00:00 +0100 753820 http://www.medworm.com/index.php?rid=753819&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12841335%26dopt%3DAbstract Authors: Aikoh H The reduction rate of mercuric ion to metallic mercury by a superoxide anion produced by a xanthine-xanthine oxidase system increased with an increased concentration of xanthine oxidase in the presence of enough xanthine. The reduction rate of mercuric ion by a superoxide anion in the presence of nitroblue tetrazolium (NBT) was proportional to the concentration of NBT. The result suggests that NBT was reduced to diformazan by a superoxide anion produced by a xanthine-xanthine oxidase system and that mercuric ion will be reduced to metallic mercury by diformazan. The reduction rate of mercuric ion was also indicative that a superoxide anion produced by an NADH-phenazine methosulfate (PMS) system increased with an increased concentration of PMS. PMID: 12841335 [PubMe... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753819 Tue, 01 Jan 2002 05:00:00 +0100 753819 http://www.medworm.com/index.php?rid=753850&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758731%26dopt%3DAbstract Authors: Edelmann L Several basic mechanisms underlying living phenomena are not really understood. Unequivocal interpretations of data concerning the following phenomena--to name but a few--are missing: cellular accumulation of potassium; cellular exclusion of sodium, cell volume regulation, shape change of cells (e.g. of muscle cells during contraction), electrical potential differences between inside and outside of living cells. The theoretical treatment of these phenomena as found in all current textbooks is based on the membrane-pump theory (MPT) with the following essential features. The bulk of the main cellular cation K+ is freely dissolved in free cellular water and membrane-situated pumps are responsible for the high level of K+ and the low level of Na+ found in virtually all... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753850 Mon, 01 Jan 2001 05:00:00 +0100 753850 http://www.medworm.com/index.php?rid=753849&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758732%26dopt%3DAbstract Authors: Litinetsky L, Rosenzweig E, Ishay JS The present study focused on temperature assessments within a hornet nest. The measurements encompassed adult hornets, brood combs and the various stages of brood, and involved a thermographic method. Body parts of adult hornets were found to vary in their temperature, with the thorax eliciting the highest temperature and the abdomen the lowest. Similarly, there were thermal variances between larvae at instars 4-5, light-colored pupae and dark pupae. The measurements were made at day and night (when the entire population was present in the nest) on nests containing thousands of individuals at various ages. Most of the pupae measured during October were hornet drones. The usual air temperature between the (subterranean) combs was 28.7 degree... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753849 Mon, 01 Jan 2001 05:00:00 +0100 753849 http://www.medworm.com/index.php?rid=753848&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758733%26dopt%3DAbstract Authors: Wang Y, Kashiba M, Kasahara E, Tsuchiya M, Sato EF, Utsumi K, Inoue M Although the coordination of various antioxidants is important for the protection of organisms from oxidative stress, dynamic aspects of the interaction of endogenous antioxidants in vivo remain to be elucidated. We studied the metabolic coordination of two naturally occurring water-soluble antioxidants, ascorbic acid (AA) and reduced glutathione (GSH), in liver, kidney and plasma of control and scurvy-prone osteogenic disorder Shionogi (ODS) rats that hereditarily lack the ability to synthesize AA. When supplemented with AA, its levels in liver and kidney of ODS rats increased to similar levels of those in control rats. Hepato-renal levels of glutathione were similar with the two animal groups except for th... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753848 Mon, 01 Jan 2001 05:00:00 +0100 753848 http://www.medworm.com/index.php?rid=753847&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758734%26dopt%3DAbstract In this study, we examined the induction of a radio-adaptive response to cell death using a colony formation test in m5S, G401.2/6TG.1 and HeLa cells. When m5S cells were subjected to priming irradiation of 0.05 to approximately 0.15 Gy 4 hr before being irradiated with 4.5 Gy, the survival ratios increased significantly to 39 to approximately 42%. The priming irradiation effect was also observed when G401.2/6TG.1 cells were subjected to priming irradiation of 0.025 to approximately 0.1 Gy 4 hr before being irradiated with 0.8 Gy. This effect showed a two-phasic characteristic, where the first peak was reached at 0.025 Gy, and the second peak was reached at 0.075 Gy. The first peak showed a survival ratio of 56%, while the second peak was at 55%. However, in HeLa cells, this priming irradi... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753847 Mon, 01 Jan 2001 05:00:00 +0100 753847 http://www.medworm.com/index.php?rid=753846&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758735%26dopt%3DAbstract Authors: Fei DY, Kraft KA, Tangwancharoenchai N, Jiang T Saturation-tagging Magnetic Resonance (MR) imaging provides a simple and robust means to directly visualize displacement profiles within fluid flow fields. Although useful for velocity quantitation as well as for qualitative depiction of flow patterns in certain well-defined flow fields, the technique is prone to distortions due to oblique flow (misregistration artifact) and ambiguity of fluid vector trajectories in complex flow situations. A novel method is proposed whereby two images are acquired, differing in the temporal position of the phase encoding gradient. Theoretical analysis shows that from the paired images, distortion of the two-dimensional displacement profile can be corrected and fluid velocity vectors extracted, e... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753846 Mon, 01 Jan 2001 05:00:00 +0100 753846 http://www.medworm.com/index.php?rid=753845&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758736%26dopt%3DAbstract Authors: de Mello MA, de Souza CT, Braga LR, dos Santos JW, Ribeiro IA, Gobatto CA The present study was designed to evaluate the effects of chronic aerobic exercise (swimming, 1h/day, 5 days/week, with an overload of 5% body weight) on glucose metabolism in obese male Wistar rats. Hypothalamic obesity was induced through administration of monosodium glutamate (MSG) at 4 mg/g of body weight every other day from birth to 14 days old. Fourteen weeks after drug administration, the rats were separated into two groups: MSG-S (sedentary) and MSG-T (swimming for 10 weeks). Rats of the same age and strain, receiving saline in place of MSG, were used as control (C), and subdivided into two groups: C-S and C-T. At the end of the experimental period, an oral glucose tolerance test was performed a... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753845 Mon, 01 Jan 2001 05:00:00 +0100 753845 http://www.medworm.com/index.php?rid=753844&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758737%26dopt%3DAbstract In conclusion, alloxan was less effective in producing hyperglycemia in rats fed a low protein diet than in normal diet rats. This effect is attributable to an increased peripheral sensivity to insulin in addition to a better preservation of glucose oxidation and insulin secretion in islets from rats fed a low protein diet. PMID: 11758737 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753844 Mon, 01 Jan 2001 05:00:00 +0100 753844 http://www.medworm.com/index.php?rid=753843&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11758738%26dopt%3DAbstract Authors: Agmon I, Litinetsky L, Gavan J, Ishay JS Our study shows that the antenna of the hornet is densely covered from base to tip with six types of organulles, namely, a trichoid organulle, about 23 microm in length, a campaniform organulle which is 12 microm long, a heretofore undescribed structure measuring about 9 microm in length which we have now named the Agmon organulle, a rather flattened placoid organulle measuring about 25 microm in length and not projecting on the antennal surface, and finally rounded structures about 3 microm in diameter which are indented in the cuticle and resemble extraretinal photoreceptors. All the above-mentioned structures occur in the workers and drones, and the latter also possess an additional structure, namely, the tyloid which is about 254 mi... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753843 Mon, 01 Jan 2001 05:00:00 +0100 753843 http://www.medworm.com/index.php?rid=753842&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002686%26dopt%3DAbstract Authors: Arita K, Kanno T, Takehara Y, Fujiwara T, Akiyama J, Horton AA, Utsumi T Polymorphonuclear leukocytes from healthy volunteers (HPMN) generated superoxide (O2*-) following treatment with various stimuli, such as phorbol myristate acetate (PMA), opsonized zymozan (OZ) and arachidonic acid (AA). Other types of n-3 polyunsaturated fatty acids (PUFAS), such as docosahexaenoic acid (DHA), docosapentaenoic acid (DPA), and eicosapentaenoic acid (EPA), also stimulated O2*- generation. The free form of DHA enhanced the generation of O2*- induced by PMA but inhibited that induced by OZ. In contrast, the ethylester of DHA (DHA-E) inhibited O2*- generation induced by PMA but stimulated that induced by OZ. Similar effects were also observed with ethylesters of EPA (EPA-E), DPA (DPA-E) and A... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753842 Mon, 01 Jan 2001 05:00:00 +0100 753842 http://www.medworm.com/index.php?rid=753841&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002687%26dopt%3DAbstract Authors: Yamaoka K, Mifune T, Mitsunobu F, Kojima S, Mori S, Shibuya K, Tanizaki Y, Sugita K Because most of the diseases to which radon (222Rn) therapy is applied are related to activated oxygen, in this study the effect of the radioactivity of radon and the thermal effect were compared under a room or a hot spring condition with the same chemical component using as the parameters the activity of superoxide dismutase (SOD), which is an oxidation inhibitor, and lipid peroxide and low density lipoprotein (LDL)-cholesterol, which are closely involved in arteriosclerosis. Results show that the SOD activity was significantly increased, and the lipid peroxide and LDL-cholesterol levels were significantly decreased on days 6 and 7 of study. The results were about 2-fold larger in the radon g... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753841 Mon, 01 Jan 2001 05:00:00 +0100 753841 http://www.medworm.com/index.php?rid=753840&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002688%26dopt%3DAbstract Authors: Kuchroo K, Maity H, Kasturi SR Tubulin, the major protein of microtubules, has been shown to be an example of protein undergoing multistep unfolding. Local unfolding and stepwise loss of a number of characteristic functions were demonstrated. In order to understand urea induced effects on tryptophan fluorescence and nucleotide binding on tubulin, both fluorescence and NMR techniques were used. Tubulin was denatured by different urea concentrations. The present experiments were carried out at concentrations of tubulin (to approximately 10 microM) at which most of the protein will be in the dimeric state. Quenching studies in the presence of KI suggest that all the tryptophans are fairly solvent exposed. Similar studies using acrylamide as quencher, suggest unfolding of tubulin ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753840 Mon, 01 Jan 2001 05:00:00 +0100 753840 http://www.medworm.com/index.php?rid=753839&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002689%26dopt%3DAbstract Authors: Menon GS, Kuchroo K, Dasgupta D Indigenous variety of Xanthium strumarium (X. strumarium) was screened for its antimitotic activity using the microtubule-tubulin system isolated from mammalian tissue. A preliminary phytochemical screening of the whole extracts of the plant was carried out followed by partial purification of the whole extract of X.strumarium. The separated fractions obtained were identified and used for in vitro polymerization studies. The whole as well as partially separated chemical constituents of X. strumarium showed effective inhibition of tubulin polymerization. The results thus suggest that X. strumarium may possess antimitotic components. PMID: 12002689 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753839 Mon, 01 Jan 2001 05:00:00 +0100 753839 http://www.medworm.com/index.php?rid=753838&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002690%26dopt%3DAbstract Authors: Dhanasekaran M, Srivastava S, Raju EB, Durani S Partial 'turn-helix' type modules comprised of LD and DL chiral beta-turns serving as potential helix nucleators have been connected with a view to designing a nascent 'helix-turn-helix' type structure. Conformation of the resultant peptide Boc-(D)Glu-Ala-Aib-Lys-Val-Pro-(D)Asp-Leu-Leu-NHMe has been described in both DMSO and water. PMID: 12002690 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753838 Mon, 01 Jan 2001 05:00:00 +0100 753838 http://www.medworm.com/index.php?rid=753837&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002691%26dopt%3DAbstract Authors: Kirshboim S, Ishay JS The present study reports on experiments carried out on workers of Vespa orientalis (Hymenoptera, Vespinae). The experiments involved: learning of the workers upon their breeding box, evaluation of their ability to navigate back to the breeding box from ever increasing distances, and the effect which masking the various light perceiving organs has on the homing flight of hornets. The test hornets were removed from their breeding box to which they became accustomed and then released at gradually increasing distances from it (100 to 1000 meters) so as to assess their ability to return home. The group of hornets returning from a given distance was on the next day released at a greater distance, and so forth, and their homing capability was then compared betw... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753837 Mon, 01 Jan 2001 05:00:00 +0100 753837 http://www.medworm.com/index.php?rid=753836&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002692%26dopt%3DAbstract Authors: Pertsis V, Litinetsky L, Sverdlov A, Rosenzweig E, Ishay JS The aim of the present investigation was two-fold: a) to observe the homing of the Oriental hornet, Vespa orientalis (Hymenoptera, Vespinae) from different distances; and b) to study the photothermoelectric activity of hornet cuticle obtained from the subjects of goal (a) and kept frozen for a number of days prior to its testing. In both the above mentioned phases of the investigation, an attempt was made to assess how the covering of the hornets' cuticle with Ultra Violet B (UVB) blockers affects their activity as compared to the control. Flying hornets were observed to return to the nest from distances of up to 7 km, once they had learned the way back. However, covering of the cuticle with UVB blockers increases the... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753836 Mon, 01 Jan 2001 05:00:00 +0100 753836 http://www.medworm.com/index.php?rid=753835&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002693%26dopt%3DAbstract Authors: Campa A, Bruneri LH, Burger E Neutrophils exposed to pro-inflammatory substances have an increased capability to respond to a membrane receptor-binding stimulus triggering an oxidative burst. One other considered sign of neutrophil activation is the appearance of a high-resolution 'H-NMR spectrum probably resulting from a rearrangement of neutral lipids in the membrane. The relationship between these two events is here studied. Neutrophils were recovered from a subcutaneous fungal infection using two murine lineages that respond differently to infection. It was concluded that rearrangement of lipids in the membrane, observable by NMR, appears also in neutrophils that do not exhibit the classical sign of activation, that is the increased expression of membrane receptors. PM... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753835 Mon, 01 Jan 2001 05:00:00 +0100 753835 http://www.medworm.com/index.php?rid=753834&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D12002694%26dopt%3DAbstract Authors: Edelmann L PMID: 12002694 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753834 Mon, 01 Jan 2001 05:00:00 +0100 753834 http://www.medworm.com/index.php?rid=753866&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970042%26dopt%3DAbstract Authors: Shukla-Dave A, Roy R, Bhaduri AP, Chatterjee RK A well known glucose antimetabolite, 2-deoxy glucose (2DG) widely used in chemotherapy of cancer along with radiation, was evaluated as an antifilarial agent by nuclear magnetic resonance. The uptake and metabolism of 2DG in the experimental filarial infection Acanthocheilonema viteae was studied by in vivo multinuclear NMR. An unusually long retention time of 2DG6P within these parasites was observed on continuous 31P NMR monitoring, along with a decrease in ATP levels. These results led to therapeutic investigation in A. viteae infected host Mastomys coucha. 2DG showed a remarkable adulticidal activity (73.6%) with 50% sterilization of surviving female worms at a dose of 250 mg/kg x 5, p.o. NMR observations and activity profile... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753866 Sat, 01 Jan 2000 05:00:00 +0100 753866 http://www.medworm.com/index.php?rid=753865&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970043%26dopt%3DAbstract In this study, we compared ethanol metabolism in human subjects dosed with non-treated white wine (control = CON) and with US treated wine. Ethanol levels in human sera were followed by 400 MHz 1H-NMR spectroscopy after administration of wine doses. Experimental results indicated that ethanol metabolism was enhanced 18% in subjects when US treated wine was used rather than when non-treated (CON) was used. Other experiments using rabbits showed that a 20% ethanol-aqueous solution was absorbed 18% more rapidly by the group dosed with US wine than by the CON group. From these experimental facts, it was theorized that ethanol metabolism depends on the rapidity of ethanol absorption in the human body. And it can be concluded that US treatment brings about the same effect on spirits or wines as ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753865 Sat, 01 Jan 2000 05:00:00 +0100 753865 http://www.medworm.com/index.php?rid=753864&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970044%26dopt%3DAbstract Authors: Nakayama K, Awata S, Zhang J, Ebinuma H, Mariyama T, Kodama H The concentrations of sulfur-containing amino acids, taurine, cystathionine, methionine and cystine, as well as cystathionine beta-synthase and gamma-lyase activities in various tissues of Agkistrodon blomhoffi (mamushi) were measured. The concentration of taurine in examined tissues was greater than the concentration of other sulfur-containing amino acids. The concentration of cystathionine in various tissues was also much higher than those of methionine and cystine, but the concentration of cystathionine in the brain was lower than that of methionine. In all tissues examined in this study, cystathionine beta-synthase activity was much higher than that of cystathionine gamma-lyase. The ratios of cystathionine beta-... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753864 Sat, 01 Jan 2000 05:00:00 +0100 753864 http://www.medworm.com/index.php?rid=753863&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970045%26dopt%3DAbstract Authors: Cardellini E, Nardicchi V, Macchioni L Total protein extract from HL-60 cells was found to be able to dephosphorylate the RNA polymerase II octapeptide pyroGlu-Asp-Asp-Ser-Asp-Glu-Glu-Asn previously phosphorylated with protein kinase CKII (pCKII). Fractionation in cytoplasm, nuclear and chromatin extracts shows the phosphatase activity to be localized only in the nucleus, but not to be bound to the chromatin. PMID: 10970045 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753863 Sat, 01 Jan 2000 05:00:00 +0100 753863 http://www.medworm.com/index.php?rid=753862&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970046%26dopt%3DAbstract Authors: Ruggiero AC, Nepomuceno MF, Jacob RF, Dorta DJ, Tabak M The antioxidant effects of dipyridamol (DIP), a coronary vasodilator, and its derivative RA-25 were compared in intact red blood cells (RBC) and in isolated ghost membranes. Both compounds are quite effective antioxidants in cumene hydroperoxide-induced lipid peroxidation of RBC, showing a much smaller effect for hydrogen peroxide oxidation. The antioxidant effect of DIP was considerably higher than that of RA25. For isolated ghost membranes, the apparent IC50 (the drug concentration that produces 50% inhibition of lipid peroxidation) in cumene hydroperoxide-induced peroxidation was 25 microM, while the maximum protective effect of RA-25 was around 30% in the drug concentration range of 50-100 microM. The drugs can protec... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753862 Sat, 01 Jan 2000 05:00:00 +0100 753862 http://www.medworm.com/index.php?rid=753861&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970047%26dopt%3DAbstract Authors: Bartzatt R, Benish T, Koziol K, Stoddard J Bacterial resistance to antibiotics is a significant problem in health facilities and results in higher costs for health care and increased fatalities due to infection. The work presented here suggests that antibiotic molecular structure can be altered in a selected manner, which will revive the bacterial growth inhibiting capability. A bacterial strain PKK3535(DH1), which is resistant to the antibiotic ampicillin, was found to be highly growth inhibited by these altered forms of ampicillin when tested in tissue culture. The level of growth inhibition of bacterial strain PKK3535(DHI) was greater than 50%, for both molecular variants of ampicillin that were investigated. The bacteria strain used for testing was a clinical isolate obtai... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753861 Sat, 01 Jan 2000 05:00:00 +0100 753861 http://www.medworm.com/index.php?rid=753860&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970048%26dopt%3DAbstract Authors: Sverdlov A, Litinetsky L, Pertsis V, Ishay JS This paper deals with the thermophotovoltaic (TPV) properties of the cuticle of the Oriental hornet as assessed over time under different regimens of relative humidity (RH). The tests were run at two levels of RH, namely, 30% vs. 90%. Each experiment entailed measuring the cuticular voltage and current in the dark as compared to under illumination (white light = 700 Lux), and at a temperature range of 20-30 degrees C. It was found that increase in the RH level boosts the current values by 2-3 orders of magnitude; contrariwise, the voltage values rise by about three times with drop in the RH. At high RH, the changes in current become rhythmical and each cycle of warming-cooling assumes a distinctly cyclic pattern. Under illumination... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753860 Sat, 01 Jan 2000 05:00:00 +0100 753860 http://www.medworm.com/index.php?rid=753859&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970049%26dopt%3DAbstract Authors: Rana SV, Rastogi N The influence of insulin-dependent diabetes mellitus on the oxidative stress caused by cadmium in the liver and kidney of laboratory rats has been studied. The results suggest that cadmium and alloxan diabetes independently promote lipid peroxidation in both liver and kidney. However, lipid peroxidation diminished in the diabetic rats fed cadmium. Administration of cadmium to normal and diabetic rats depleted glutathione in liver only. No significant change was observed in the activity of glutathione peroxidase in kidney, whereas administration of cadmium to diabetic rats stimulated catalase activity when compared to cadmium-fed rats. The actual mechanism of these effects still remains to be confirmed, but an antagonistic relationship between cytotoxic mecha... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753859 Sat, 01 Jan 2000 05:00:00 +0100 753859 http://www.medworm.com/index.php?rid=753858&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10970050%26dopt%3DAbstract Authors: Yaman A The effects of J modulation on localized 1H NMR spectra were observed on metabolites with both a short T2 relaxation time like glutamate and a comparatively long T2 relaxation time like N-acetyl-L-aspartic acid (NAA). All examinations were performed by either the double spin-echo (PRESS) sequence or J-suppressed PRESS (JPRESS) sequence. Both of the sequences allow spectral editing and relaxation time studies of J coupled spins. Water-suppressed proton spectra were obtained at 1.5 T (GE Signa) from a 6-8 ml volume of interest. T1 and T2 relaxation times of some of the major brain metabolites were obtained by varying the repetition time and echo time of the JPRESS sequence. PMID: 10970050 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753858 Sat, 01 Jan 2000 05:00:00 +0100 753858 http://www.medworm.com/index.php?rid=753857&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383133%26dopt%3DAbstract Authors: Yasuda M, Sugahara K, Zhang J, Shuin T, Kodama H Production of guanidinoacetic acid, a precursor of creatinine is known to be reduced by metabolic disturbance when kidney function is damaged, and thus it may be a sensitive marker of renal damage. Therefore, the urinary levels of guanidinoacetic acid, creatinine and creatine from patients with urinary tract neoplasm who received cisplatin treatment were measured by liquid chromatography-mass spectrometry. Following the administration of cisplatin, the urinary excretion of guanidinoacetic acid decreased significantly, and the low concentration was maintained for at least five days. The concentrations of creatinine and creatine gradually decreased until the third day after cisplatin administration, and slightly increased on the f... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753857 Sat, 01 Jan 2000 05:00:00 +0100 753857 http://www.medworm.com/index.php?rid=753856&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383134%26dopt%3DAbstract We report here the effect of L-arginine on membrane lipid peroxidation of goat epididymal spermatozoa. Both natural peroxidation as well as that induced by UV radiation, freezing and oxidizing agents have been studied. Irrespective of the nature of induction of peroxidation, L-arginine reduces the extent of lipid peroxidation in a concentration dependent manner. Both L-arginine and alpha-tocopherol act synergistically in protecting against lipid peroxidation induced by the above methods. Thus, in order to provide protection against lipid peroxidation, L-arginine may be added in media used to preserve spermatozoa. PMID: 11383134 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753856 Sat, 01 Jan 2000 05:00:00 +0100 753856 http://www.medworm.com/index.php?rid=753855&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383135%26dopt%3DAbstract Authors: Ravera R, Allegra P, Colombatto S, Solinas SP This work is the first demonstration that cystamine is actively accumulated in spheroplasts of Saccharomyces cerevisiae. We have identified and quantitatively determined the transported cystamine in extracts of spheroplasts that have been incubated over different time periods and in the presence of different amounts of cystamine. The method used, already reported in literature for the identification of natural aliphatic polyamines in biological fluids, consists of a derivatization of spheroplast extracts with dabsyl-chloride and subsequent chromatographic analysis in HPLC. Our results show that cystamine accumulation is a function of time, it increases up to 2.5 min then decreases. Transport is inhibited by natural aliphatic polyam... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753855 Sat, 01 Jan 2000 05:00:00 +0100 753855 http://www.medworm.com/index.php?rid=753854&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383136%26dopt%3DAbstract Authors: Justo GZ, Camargo FA, Haun M, Faljoni-Alário A, Durán N Xanthine oxidase (XO) has been investigated for its decreased activity in several cancerous tissues and constitutive generation of reactive oxygen species (ROS) in vivo seems to contribute significantly to its inactivation. Singlet oxygen (1O2) production has been suggested to be relevant when considering folic acid metabolism by cancer cells. Thus, the susceptibility of XO to inactivation by 1O2 generated either by the bioenergized systems folic acid/peroxidase/GSH/Mn2+/O2 and malonaldehyde/peroxidase/Mn2+/O2 or by methylene blue (MB) or eosin-sensitized photooxygenation was studied. Our results showed that other ROS were also responsible for XO inactivation when MB was used. In contrast, eosin produced almost exclusiv... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753854 Sat, 01 Jan 2000 05:00:00 +0100 753854 http://www.medworm.com/index.php?rid=753853&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383137%26dopt%3DAbstract Authors: Rudan-Tasic D, Jurca S, Klofutar C The static electric permittivity and refractive index of carbon tetrachloride solutions of ethyl-2-methyl butanoate, 3-hexene-l-ol, 2-hexenal, ethyldecanoate, methyl anthranilate, anethole, damascenone and isoamyl propanoate were measured at 298.15 K in the concentration range up to 4.0 mol dm-3. The molar polarizations and refractions of the solutions, the partial molar polarizations and refractions of the solutes, as well as the dipole moments at infinite dilution of the solutes were calculated. On the basis of the concentration dependence of apparent values of the square of the molecular dipole moment, it was established that for the systems studied oligomeric species with low dipole moment were prevalent. With the assumption that the dipo... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753853 Sat, 01 Jan 2000 05:00:00 +0100 753853 http://www.medworm.com/index.php?rid=753852&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383138%26dopt%3DAbstract Authors: Matveev VV Actin filaments are certainly believed to function as an intracellular signalling system; however, this is not confirmed by direct evidence. We used a two-layer actomyosin gel with a concentration gradient of the troponin-tropomyosin complex (TT-complex, Ca(2+)-sensitive system) between the two layers. To prepare one layer of the system, natural actomyosin (nAM) rich in TT-complex was used. To prepare the second layer, we used desensitized actomyosin (dAM) without the complex. All experimental studies were made in medium with a low ionic strength. Two phenomena were observed: (1) dAM blocks Ca(2+)-sensitivity of nAM when the dAM weight portion in the system (as well as in mixed nAM + dAM suspension) reaches 40% and more; further increase of the dAM portion does not ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753852 Sat, 01 Jan 2000 05:00:00 +0100 753852 http://www.medworm.com/index.php?rid=753851&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D11383139%26dopt%3DAbstract Authors: Iwazumi T Muscle is one of few organs whose performance can be measured by physical quantities. However, very few attempts have been made to apply theoretical physics to muscle. In this paper we will see how physical principles can be applied by taking advantage of unique properties of muscle structure. The first topic is to establish the stability conditions of sarcomere structure. The conclusions are then compared to some experimental facts. Next, we move on to the field theory fundamentals. The concept of energy density as a stress tensor is shown to be a powerful tool for the dielectric force theory to understand how proteins move under electric fields. By combining the structural stability theory and the dielectric force theory we arrive at a helical dipole array. We disc... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753851 Sat, 01 Jan 2000 05:00:00 +0100 753851 http://www.medworm.com/index.php?rid=753873&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10535102%26dopt%3DAbstract Authors: Yamaoka K, Kojima S, Nomura T We demonstrated that low-dose irradiation with 50 cGy of X-ray induces in vivo production of superoxide dismutase (SOD)-like substances and accelerates antioxidant activity. To elucidate the defense mechanism against X-ray radiation, we examined which components among these SOD-like substances, such as SOD, vitamin C and celuroplasmin, are produced by low-dose irradiation. Our study revealed that SOD-like specific activity hardly involved SOD-like substances other than SOD. Moreover, it is suggested that low-dose irradiation induced synthesis and production of SOD itself, leading to elevation of SOD-specific activity. PMID: 10535102 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753873 Fri, 01 Jan 1999 05:00:00 +0100 753873 http://www.medworm.com/index.php?rid=753872&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10535103%26dopt%3DAbstract Authors: Patel AB, Srivastava S, Phadke RS, Govil G It is known that the amino acid arginine stimulates sperm motility and glycolytic activity. We have earlier studied its efficacy as a stimulator of glycolysis in goat spermatozoa under anaerobic conditions. Here, we have assessed the influence of arginine in reversing the impairment caused by glycolytic inhibitors, iodoacetamide and iodoacetic acid. Glycolysis has been monitored by measuring the consumption of 13C labeled glucose and the amount of 13C labeled lactate produced under different experimental conditions, using 13C NMR. It is observed that both L- and D-arginine are able to prevent and reverse the inhibitory action of glycolytic inhibitors. The reversal effect of arginine gives rise to about eight times higher metabolic act... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753872 Fri, 01 Jan 1999 05:00:00 +0100 753872 http://www.medworm.com/index.php?rid=753871&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10816760%26dopt%3DAbstract Authors: Yabuki M, Miyake T, Doi Y, Fujiwara T, Hamazaki K, Yoshioka T, Horton AA, Utsumi K Nuclear breakdown leading to the formation of apoptotic bodies has been postulated to involve degradation of nuclear structural proteins, such as lamins A/C and B. Although nuclear segmentation occurs during the maturation of polymorphonuclear leukocytes (neutrophils), its mechanism is not known. We found that human neutrophils have lamin B but lack lamins A/C while mononuclear cells possess all three types of lamin as assessed by immunoblotting. Differentiation of human promyelocytic HL-60 cells into neutrophil-like cells was also accompanied by the down-regulation of lamins A/C but not of lamin B. Moreover, when compared with normal cells, neutrophils with the Pelger-Huët anomaly of nuclear h... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753871 Fri, 01 Jan 1999 05:00:00 +0100 753871 http://www.medworm.com/index.php?rid=753870&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10816761%26dopt%3DAbstract We report formation of ion channels in lipid bilayer membranes by bis[(benzo-15-crown-5)-15-yl methyl] pimelate, a crown ether known to form ion inclusion complexes with alkali metal cations. The channels have characteristic long openings lasting several seconds and a low conductance (4 pS in 500 mM KCl and 2.5 pS in 500 mM NaCl). A model of the crown ether channel formed by stacking of four monomers is proposed. A large database of structural information on crown ethers and their ion inclusion complexes as well as large family of crown ethers with a variety of substitutions in the ring are commercially available. Thus the crown ether channel is an attractive model system to study the role of various chemical moieties in ion conduction which may provide deeper insight into understanding th... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753870 Fri, 01 Jan 1999 05:00:00 +0100 753870 http://www.medworm.com/index.php?rid=753869&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10816762%26dopt%3DAbstract Authors: Nishina M, Kato T, Ito M, Takashima S In the development of the human cerebellum, the intracellular metabolites were monitored during the period from the fetus to childhood by in vitro high resolution proton (1H) magnetic resonance (MR) spectroscopy. The spectra from fetus (15-30 post-menstrual weeks; n = 3), infant (1-24 months of age; n = 6) and child (7-14 years of age; n = 5) groups showed resonances from seventeen different metabolites. The level of N-acetylaspartate (NAA), one of the metabolites, was observed in age-dependent increases, two- and three-fold increases for infant and child groups from the NAA of the fetus group, respectively. The rapid increases in the creatine (Cre) level (approximately three-fold) in the fetus and infant groups were observed in the child ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753869 Fri, 01 Jan 1999 05:00:00 +0100 753869 http://www.medworm.com/index.php?rid=753868&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10816763%26dopt%3DAbstract The objective of this study was to investigate the effects of ultrasound treatment and physical exercise on the velocity of bone consolidation and resistance to deformation. We performed osteotomy in the upper third of the right tibia of rats. Physical training consisted of swimming 1 h per day with a load of 5% b.w. for 30 days. Therapy with medium-intensity ultrasound was applied daily on the damaged area. Wistar rats were divided into the following groups: osteotomized sedentary animals with no ultrasound treatment (1.OSnUS), osteotomized trained animals with no ultrasound treatment (2.OTnUS), osteotomized sedentary animals with ultrasound treatment (3.OSwUS), and osteotomized trained animals with ultrasound treatment (4.OTwUS). The animals were sacrificed for the following analyses: mu... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753868 Fri, 01 Jan 1999 05:00:00 +0100 753868 http://www.medworm.com/index.php?rid=753867&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10816764%26dopt%3DAbstract Authors: Danielyan AA, Mirakyan MM, Grigoryan GY, Ayrapetyan SN Radioactive labeled ouabain was used for estimating the static magnetic field (SMF) induced cell volume changes. Ouabain is a specific inhibitor of Na+/K+ ATPase, and can be used for estimating its quantity--thus giving information about the cell volume changes. Ouabain binding by cancer and normal glandular tissues of breast cancer patients and normal glandular tissues of healthy women was measured after exposure of tissues to SMF 0.2T. SMF exposure led to a decrease of ouabain binding in both normal and cancer tissues when ouabain concentration in the external medium was 10(-9) M, while in the case of higher concentrations of ouabain (10(-7) M, 10(-6) M) an increase of ouabain binding was seen. The normal glandular tissu... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753867 Fri, 01 Jan 1999 05:00:00 +0100 753867 http://www.medworm.com/index.php?rid=753885&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807233%26dopt%3DAbstract Authors: Yamaoka K, Nomura T, Iriyama K, Kojima S Blood activities of hepatocellular enzymes such as lactate dehydrogenase (LDH), glutamic pyruvic transaminase (GPT) and glutamic oxalacetic transaminase (GOT) peaked at 12 hours after a single intraabdominal injection of ferric nitrilotriacetate (Fe(3+)-NTA) in rats. Enzymes such as alkaline phosphatase (ALP) and leucin amino peptidase (LAP) originating in the capillary bile ducts or bile secretory liver cells were also released into the blood between 6-24 hours after intraabdominal injection of Fe(3+)-NTA in rats. Furthermore, hyperoxidation of lipids occurred in rat hepatic cell membranes, reaching a peak 6 hours after intraabdominal injection of Fe(3+)-NTA. It was found that a single prior 0.5 Gy whole body X-ray irradiation signific... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753885 Thu, 01 Jan 1998 05:00:00 +0100 753885 http://www.medworm.com/index.php?rid=753884&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807234%26dopt%3DAbstract Authors: Czerlinski G, Levin R, Ypma T The kinetics of the reaction of hemoglobin with molecular oxygen, in which rapid mixing is followed by a very fast temperature jump, is numerically simulated. Values for rate constants are used to the extent known, otherwise interpolated or extrapolated. It is shown that reaction steps not resolvable by rapid mixing can be resolved by subsequent chemical relaxation at appropriate points in time. Four different mechanisms are considered, all assuming no distinction between the two kinds of chains of hemoglobin. Bimolecular rate constants for oxygen binding are either the same for all four sites, or are governed by "frequency factors" (the kinetic equivalent of statistical factors for equilibrium constants in allosteric models). Furthermore, either ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753884 Thu, 01 Jan 1998 05:00:00 +0100 753884 http://www.medworm.com/index.php?rid=753883&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807235%26dopt%3DAbstract Authors: Maity H, Jarori GK The conformation of ADPMg(II) bound at the active site of yeast hexokinase PI has been determined using transferred nuclear Overhauser effect spectroscopy (TRNOESY). We have measured the time dependent NOE buildup of all the proton pairs of ADP in enzyme. ADPMg(II) and enzyme. glucose.ADPMg(II) complexes at 500 MHz and 10 degrees C. The data have been analyzed using complete relaxation matrix approach to obtain various inter-proton distances. These distances were used as restraints in the molecular dynamics and energy minimization to obtain the conformation of the bound nucleotide. The results from these calculations suggest that in both the complexes, the nucleotide binds in an anti conformation with a glycosidic torsion angle chi = 55 +/- 5 degrees and 52 ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753883 Thu, 01 Jan 1998 05:00:00 +0100 753883 http://www.medworm.com/index.php?rid=753882&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807236%26dopt%3DAbstract Authors: Bogner P, Nagy E, Miseta A The regulation of cellular ion levels has been an important issue of cell physiology since the beginning of the century. A special interest was focused on the monovalent ions which are involved in several cellular functions; in fact, the maintenance of high K+ level inside the cells is one of the most basic life-phenomena. Regarding the regulation of monovalent ions in general, two opposing ideas emerged: one being the membrane theory and the other the sorption theory(ies). Today most scientists are familiar only with the membrane theory which involves the pump and leak hypothesis and only a few consider the predictions of the association-induction hypothesis which may be classified as one of the sorption theories. In the regulation of monovalent ion... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753882 Thu, 01 Jan 1998 05:00:00 +0100 753882 http://www.medworm.com/index.php?rid=753881&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807237%26dopt%3DAbstract Authors: Ling GN In response to the request of the authors of the preceding paper, this article explains three observations they and others had made in the context of the association-induction hypothesis. (1) Why is Rb+ accumulated in human red blood cells not released when transferred to a Rb(+)-free Hank's solution? (2) Why ouabain, which reduces Rb+ uptake by red blood cells, does not release this ion from Rb(+)-loaded red blood cells? (3) Why is there a positive correlation between the K+ contents of the red blood cells of different mammals and the Na,K-ATPase isolated from the red blood cells of the same mammals? PMID: 9807237 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753881 Thu, 01 Jan 1998 05:00:00 +0100 753881 http://www.medworm.com/index.php?rid=753880&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D9807238%26dopt%3DAbstract The objective of this investigation was to evaluate the effect of daily intakes of water and salt on water and electrolyte changes and body hydration status of rats during prolonged restriction of motor activity (hypokinesia). Ninety Wistar rats weighing 370 to 390 g were used to perform the studies: They were equally divided into three groups: 1. Unsupplemented vivarium control rats (UVCR); 2 Unsupplemented hypokinetic rats (UHKR) and 3. Supplemented hypokinetic rats (SHKR). For the simulation of the effect of hypokinesia (HK), the UHKR and SHKR groups were kept in small individual cages made of wood, which restricted their movements in all directions without hindering food and water intake. The SHKR received daily an additional amount of 5 ml water/100 g body weight and 3 g sodium chlori... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753880 Thu, 01 Jan 1998 05:00:00 +0100 753880 http://www.medworm.com/index.php?rid=753879&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197355%26dopt%3DAbstract Authors: Nagy S, Paál M, Kõszegi T, Ludány A, Kellermayer M In spite of the well known significance of ATP in the energy dependent life processes, the role of ATP in maintaining cellular integrity is poorly understood. A possible model for studying ATP dependent life processes is to monitor the kinetics of changes seen intra/extracellularly during ATP depletion. In our model system anticoagulated human whole blood was incubated at different temperatures to reduce intracellular ATP without addition of any chemicals. The red blood cells in their own plasma were incubated for several days at 4 degrees C or at 37 degrees C, and ATP, glucose, K+, Na+, hemoglobin, water content, mean corpuscular volume (MCV), pH and Ca2+ were analyzed in time-sequences. All the examined parameters remaine... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753879 Thu, 01 Jan 1998 05:00:00 +0100 753879 http://www.medworm.com/index.php?rid=753878&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197357%26dopt%3DAbstract Authors: Soós H, Bujáky C, Kiss A, Kovács E, Somoskeöy S, Henics T With the aid of sequential detergent fractionation and label transfer techniques, in this study we monitored the distribution of cytoplasmic AU-rich sequence binding proteins (AUBP) in different cell types. We show here that cells of various origin display diverse AUBP profiles when the presence and abundance of AUBPs were compared in two major cytoplasmic compartments, the non-ionic detergent-soluble and -insoluble fractions. We also demonstrate that different RNA probes derived from AU-rich 3'-UTR elements of various cytokine and proto-oncogene mRNAs detect distinct AUBPs within the same cell. When the in vivo association of these proteins with AU-rich RNA was assessed using the combination of in vivo UV-crosslink... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753878 Thu, 01 Jan 1998 05:00:00 +0100 753878 http://www.medworm.com/index.php?rid=753877&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197358%26dopt%3DAbstract Authors: Akber SF This paper summarizes the observations of different studies concerning the influence of paramagnetic ions on spin-lattice relaxation times. Neither the comparison between different organs, different animals nor the comparison between different tissues (normal and malignant) showed correlation of practical consequences between the paramagnetic ion concentrations in whole tissues and spin-lattice relaxation times. PMID: 10197358 [PubMed - indexed for MEDLINE] (Source: Physiological chemistry and physics and medical NMR) Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753877 Thu, 01 Jan 1998 05:00:00 +0100 753877 http://www.medworm.com/index.php?rid=753876&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197359%26dopt%3DAbstract The objective of this investigation was to measure the effect of prolonged restriction of motor activity (hypokinesia) of rats on the mass, density, mineral composition, reconstruction parameters and elemental composition of their bone tissue. The studies were done during 90 days of hypokinesia (HK) on 90 male Wistar rats equally divided into two groups: (1) vivarium control rats (VCR) and (2) hypokinetic rats (HKR). For the simulation of the hypokinetic effect the HKR group was kept for 90 days in small individual cages made of wood that restricted the movements of rats in all directions without hindering food and water intakes. During the prehypokinetic period of 15 days and during the hypokinetic period of 90 days bone mass, bone density, bone calcium and phosphorus concentrations, bone... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753876 Thu, 01 Jan 1998 05:00:00 +0100 753876 http://www.medworm.com/index.php?rid=753875&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197360%26dopt%3DAbstract Authors: Zorbas YG, Popov VK, Naexu KA, Kakurin VJ, Federenko YF The aim of this study was to examine the structural changes of glomerular and juxta glomerular system (JGS) of the kidney of rats during prolonged restriction of motor activity (hypokinesia). The studies were performed during 90 days of hypokinesia (HK) on 144 male Wistar rats divided into two groups: Group one placed under ordinary vivarium conditions and serving as vivarium control rats (VCR) and Group two subjected to HK and serving as hypokinetic rats (HKR). For the simulation of the hypokinetic effect the HKR group was kept in small individual cages made of wood that restricted the movements of rats in all directions without hindering food and water intake. During a prehypokinetic period of 15 days and the hypokineti... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753875 Thu, 01 Jan 1998 05:00:00 +0100 753875 http://www.medworm.com/index.php?rid=753874&cid=s_35856_75_f&fid=35856&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D10197356%26dopt%3DAbstract Authors: Wilson JM, Tanko Q, Wendland MM, Meany JE, Nedved JF, Pocker Y The present work demonstrates that the high-activity zinc metalloenzyme, carbonic anhydrase (CA II) from bovine erythrocytes is inhibited by the cyclic sulfimide, saccharin, and 2- and 4-carbobenzoxybenzene sulfonamide. A spectrophotometric method was employed to monitor the enzymatically catalyzed hydrolysis of p-nitrophenyl acetate by following the increase in absorbance at 410 nm which accompanies p-nitrophenoxide/p-nitrophenol formation. The more rapid enzymatic hydration of CO2 was monitored by using a stopped-flow spectrophotometer as well as by a modified colorimetric method of Wilbur and Anderson. The studies show that, at a given molar ratio of inhibitor to enzyme, the degree of inhibition of the enzymaic ... Physiological chemistry and physics and medical NMR journals http://www.medworm.com/rss/comments.php?id=753874 Thu, 01 Jan 1998 05:00:00 +0100 753874