MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Proteome Science' source. Mon, 06 Feb 2012 22:09:28 +0100 http://www.medworm.com/index.php?rid=5657269&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F6 Conclusions: SMF (200 mT) seems to maintain the cell envelope integrity and to submit the exposed cells to an oxidative stress. Some alterations suggest an increase of the ability of exposed cells to form biofilms. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5657269 Fri, 03 Feb 2012 05:00:00 +0100 5657269 http://www.medworm.com/index.php?rid=5636054&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F5 Conclusions: We identified subsets of proteins with clearly concordant/discordant behavior between gliomas and adenocarcinomas. In general, monolayer to 3D culture differences are clearer than normoxia to hypoxia differences, with anti-apoptotic, cytoskeletal rearrangement and cell survival pathways emphasized in the former and mTOR pathway, transcription, cell-cycle arrest modulation, and increased cell motility in the latter. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5636054 Wed, 25 Jan 2012 05:00:00 +0100 5636054 http://www.medworm.com/index.php?rid=5594144&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F3 Conclusion: Our results showed that diverse cellular and molecular processes were affected during somatic to embryogenic transition. Differential proteomic analysis suggests a key role for ascorbate metabolism during early stage of SE, and points to the possible role of ascorbate-glutathione cycle in establishing somatic embryos. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5594144 Fri, 13 Jan 2012 05:00:00 +0100 5594144 http://www.medworm.com/index.php?rid=5594143&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F4 Conclusion: A streamlined, functional approach to genome-wide screening of a biological activity in vivo is presented. Its application to screening in mice for an immune activity elicited by the pathogen genome of Parapoxvirus ovis yielded a novel immunomodulator. In this inverted discovery method, it was possible to identify the adjuvant responsible for a function of interest prior to a mechanistic study of the adjuvant. The non-specific immune activity of this modulator, B2, is similar to that associated with administration of inactivated particles to a host or to a live viral infection. Administration of B2 may provide the opportunity to significantly impact host immunity while being itself only weakly recognized. The functional genomics method used to pinpoint B2 within an ORFeome may ... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5594143 Fri, 13 Jan 2012 05:00:00 +0100 5594143 http://www.medworm.com/index.php?rid=5577169&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F1 Conclusion: The results from MALDI-TOF and Real Time PCR agreed; therefore, this method for identifying biomarkers of electrically stimulated chicken hearts provides a novel approach for differentiation the hearts of increased electrically stimulated chickens from those of non-stunned chickens. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5577169 Tue, 10 Jan 2012 05:00:00 +0100 5577169 http://www.medworm.com/index.php?rid=5577168&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F10%2F1%2F2 Conclusion: The results reveal that the predicted PPI networks are considerably reliable. The present research is an important pioneering work on protein function research. The porcine PPI data set, the confidence score of each interaction and a list of related data are available at (http://pppid.biositemap.com/). (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5577168 Tue, 10 Jan 2012 05:00:00 +0100 5577168 http://www.medworm.com/index.php?rid=5557053&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F76 Conclusions: By proteomic approaches including SELDI technology, we have demonstrated that intracellular S100A10 protein expression levels in drug-untreated CRC cells differ according to cell lines and are significantly correlated with sensitivity of CRC cells to L-OHP exposure. Our findings provide a new clue to searching predictive markers of the response to L-OHP, suggesting that S100A10 is expected to be one of the candidate protein markers. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5557053 Fri, 30 Dec 2011 05:00:00 +0100 5557053 http://www.medworm.com/index.php?rid=5513101&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F75 Conclusion: CEER can be multiplexed to analyze pathway proteins in a comprehensive manner with extreme specificity and sensitivity. This format is ideal for analyzing clinical samples with limited availability. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5513101 Thu, 15 Dec 2011 05:00:00 +0100 5513101 http://www.medworm.com/index.php?rid=5484130&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F74 Conclusions: It was concluded that dimethyl sulfoxide and water extracts of Turkish propolis may have anti-proliferative activity through differentiating protein expression profile in PC-3 prostate cancer cell lines along with their antioxidant capacity. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5484130 Wed, 07 Dec 2011 05:00:00 +0100 5484130 http://www.medworm.com/index.php?rid=5418667&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F73 Conclusion: The variability analysis of antibody arrays highlights the importance to consider variability components and their relative contributions when designing and evaluating studies for biomarker discover with exploratory, high-throughput and multiplexed methods. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5418667 Thu, 17 Nov 2011 05:00:00 +0100 5418667 http://www.medworm.com/index.php?rid=5418668&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F72 Conclusions: The most interesting observation in this study is that distinctly different patterns of protein expression were found in WT and pin2 mutant roots subjected to altered gravity conditions. The data also demonstrate that PIN2 mutation not only affects the basipetal transport of auxin to the elongation zone, but also results in an altered expression of proteins in the root columella. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5418668 Wed, 16 Nov 2011 05:00:00 +0100 5418668 http://www.medworm.com/index.php?rid=5418669&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F71 This study was undertaken to investigate the effects of heat inactivation of FCS and LPS contamination on the human T lymphoblast proteome. Human T lymphoblastic leukaemia (CCRF-CEM) cells were grown in FCS, either non-heated, or heat inactivated, having low ( (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5418669 Tue, 15 Nov 2011 05:00:00 +0100 5418669 http://www.medworm.com/index.php?rid=5418670&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F70 Conclusion: The control of Rac2-mediated degranulation in neutrophils likely functions through actin remodelling via activation of several actin-binding proteins. We found coronin-1A to be a novel downstream effector protein of this pathway that is threonine phosphorylated in response to secretagogue stimulation. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5418670 Mon, 14 Nov 2011 05:00:00 +0100 5418670 http://www.medworm.com/index.php?rid=5397629&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F69 Conclusions: The differentially expressed proteins confirm a switch in the substrate preference from fatty acids to other sources in the failing heart. Failing hearts showed downregulation of the major calcium transporters SERCA2 and ryanodine receptor 2 and altered expression of creatine kinases. Decreased expression of two NADPH producing proteins suggests a reduced redox reserve. Overexpression of annexins supports their possible potential as HF biomarkers. Most importantly, among the most up-regulated proteins in ACF hearts were monoamine oxidase A and transglutaminase 2 that are both potential attractive targets of low molecular weight inhibitors in future HF therapy. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5397629 Fri, 11 Nov 2011 05:00:00 +0100 5397629 http://www.medworm.com/index.php?rid=5376949&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F68 Conclusions: RBP4 induction is in agreement with known RBP4 regulation by amantadine and cisplatin. Since retinol metabolism is disrupted in many cancers and inversely associates with malignancy, these data identify a potential novel mechanism for the action of LA-12 and other similar anti-cancer drugs. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5376949 Mon, 31 Oct 2011 04:00:00 +0100 5376949 http://www.medworm.com/index.php?rid=5376950&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F67 Conclusions: These differences are likely to enhance AM function. The observations show for the first time that acute in vivo SP-A treatment of KO mice, under basal or unstimulated conditions, affects the expression of multiple AM proteins, alters F-actin distribution, and can restore much of the WT phenotype. We postulate that the SP-A-mediated expression profile of the AM places it in a state of "readiness" to successfully conduct its innate immune functions and ensure lung health. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5376950 Fri, 28 Oct 2011 04:00:00 +0100 5376950 http://www.medworm.com/index.php?rid=5331509&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F66 Conclusion: Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C.acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5331509 Tue, 18 Oct 2011 04:00:00 +0100 5331509 http://www.medworm.com/index.php?rid=5311692&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F65 Conclusions: The acetone precipitation method and the combined treatment effect of acetone and 2-DE Clean-Up Kit are not preferred in preparing SF samples for 2-DE analysis as both protein intensities and numbers decrease significantly. On the other hand, 2-D Clean-Up Kit treated SF samples revealed clearer isoforms and higher intensities for the less abundant proteins of haptoglobin, apolipoprotein A-IV, prostaglandin-D synthase, alpha-1B-glycoprotein, and alpha-2-HS-glycoprotein on 2-DE gels. As a result, it is recommended that SF samples should be treated with protein clean up products such as 2-D Clean-Up Kit first before conducting proteomic research in searching for the relevant biomarkers associated with knee osteoarthritis. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5311692 Tue, 11 Oct 2011 04:00:00 +0100 5311692 http://www.medworm.com/index.php?rid=5294138&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F63 Hodgkin lymphoma (HL) represents a category of lymphoid neoplasms with unique features, notably the usual scarcity of tumour cells in involved tissues. The most common subtype of classical HL, nodular sclerosis HL, characteristically comprises abundant fibrous tissue stroma. Little information is available about the protein composition of the stromal environment from HL. Moreover, the identification of valid protein targets, specifically and abundantly expressed in HL, would be of utmost importance for targeted therapies and imaging, yet the biomarkers must necessarily be accessible from the bloodstream. To characterize HL stroma and to identify potentially accessible proteins, we used a chemical proteomic approach, consisting in the labelling of accessible proteins and their subsequent pu... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5294138 Wed, 05 Oct 2011 04:00:00 +0100 5294138 http://www.medworm.com/index.php?rid=5294137&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F64 Background: Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients. Results: A total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5294137 Wed, 05 Oct 2011 04:00:00 +0100 5294137 http://www.medworm.com/index.php?rid=5282230&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F62 Conclusion: The described method was effective at unveiling chromatin-associated proteins that are less likely to be detected in the absence of fractionation. Several novel proteins with altered abundance were identified including Yrb1, pointing to a role for this nuclear import associated protein in DNA damage response. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5282230 Tue, 04 Oct 2011 04:00:00 +0100 5282230 http://www.medworm.com/index.php?rid=5269873&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F61 Conclusion: This is the first report conducting comprehensive identification of rice grain proteins. With a label free shotgun proteomic approach, we identified large number of rice grain proteins and compared the expression patterns of reproducibly identified proteins during rice grain development. Clustering analysis, Genome Ontology category enrichment analysis, and the analysis of composite expression profiles revealed dynamic changes of metabolisms during rice grain development. Interestingly, we detected that proteins involved in glycolysis, TCA-cycle, lipid metabolism, and proteolysis accumulated at higher levels in fully mature grain compared to grain developing stages, suggesting that the accumulation of these proteins during the desiccation stage may be associated with the prepar... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5269873 Fri, 30 Sep 2011 04:00:00 +0100 5269873 http://www.medworm.com/index.php?rid=5269874&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F60 Conclusion: Our results identify specific differences in ERbeta-interacting proteins in lung adenocarcinoma cells corresponding to ligand-dependent differences in estrogenic responses. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5269874 Tue, 27 Sep 2011 04:00:00 +0100 5269874 http://www.medworm.com/index.php?rid=5257834&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F59 Parkinson disease (PD) is histologically well defined by its characteristic degeneration of dopaminergic neurons in the substantia nigra pars compacta. Remarkably, divergent PD-related mutations can generate comparable brain region specific pathologies. This indicates that some intrinsic region-specificity respecting differential neuron vulnerability exists, which codetermines the disease progression. To gain insight into the pathomechanism of PD, we investigated protein expression and protein oxidation patterns of three different brain regions in a PD mouse model, the PINK1 knockout mice (PINK1-KO), in comparison to wild type control mice. The dysfunction of PINK1 presumably affects mitochondrial turnover by disturbing mitochondrial autophagic pathways. The three brain regions investigate... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5257834 Fri, 23 Sep 2011 04:00:00 +0100 5257834 http://www.medworm.com/index.php?rid=5247025&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F58 Conclusions: We observed overexpression of proteins related to quorum sensing, proving the existence of communication between cells, and thus the development of structuring the biofilm (mature biofilm) leading to obstruction of vessels and development of disease. This paper reports a first proteomic analysis of mature biofilm of X. fastidiosa, opening new perspectives for understanding the biochemistry of mature biofilm growth in a plant pathogen. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5247025 Thu, 22 Sep 2011 04:00:00 +0100 5247025 http://www.medworm.com/index.php?rid=5247026&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F57 Conclusion: The emerging use of MPA in diverse pathophysiological conditions demands in-depth studies to understand molecular basis of its therapeutic response. The present study identifies the myosin regulatory light chain 2 and peroxiredoxin 1 along with 10 other proteins showing significant regulation by MPA. Further characterization of these proteins may help to understand the diverse cellular effects of MPA in addition to its immunosuppressive activity. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5247026 Tue, 20 Sep 2011 04:00:00 +0100 5247026 http://www.medworm.com/index.php?rid=5234385&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F55 In this study we performed SELDI-ToF-MS analysis on serum samples treated with the ProteoMinerTM kit, a combinatorial library of hexapeptide ligands coupled to beads, to reduce the wide dynamic range of protein concentration in the sample. Serum from 44 NSCLC patients and 19 healthy controls were analyzed with IMAC30-Cu and H50 ProteinChip Arrays. Results: Comparing SELDI-ToF-MS protein profiles of NSCLC patients and healthy controls, 28 protein peaks were found significantly different (p (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5234385 Mon, 19 Sep 2011 04:00:00 +0100 5234385 http://www.medworm.com/index.php?rid=5234384&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F56 Conclusions: Despite the in depth 2DE approach used in this study the results underline the deficiencies of gel-based proteomics for detection of plasma biomarkers. Gel-free approaches may be more productive to increase the number of plasma biomarkers for DS for non-invasive prenatal screening and diagnosis. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5234384 Mon, 19 Sep 2011 04:00:00 +0100 5234384 http://www.medworm.com/index.php?rid=5234386&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F54 Conclusions: This study provides the first description of mare follicular fluid proteome during the late follicle development stages. We identified several proteins from crude, depleted and enriched follicular fluid. Our results demonstrate that the enrichment method, combined with 2D-PAGE and mass spectrometry, can be successfully used to visualize and further identify the low-abundance proteins in the follicular fluid. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5234386 Sat, 17 Sep 2011 04:00:00 +0100 5234386 http://www.medworm.com/index.php?rid=5234387&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F53 An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots. The signals are not linear if using the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5234387 Thu, 15 Sep 2011 04:00:00 +0100 5234387 http://www.medworm.com/index.php?rid=5204969&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F52 Conclusion: Our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5204969 Wed, 07 Sep 2011 04:00:00 +0100 5204969 http://www.medworm.com/index.php?rid=5191704&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F50 Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5191704 Wed, 31 Aug 2011 23:00:00 +0100 5191704 http://www.medworm.com/index.php?rid=5156757&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F49 Conclusions: Proteomic analysis enabled us to establish PPP and the talin-pFAK as targets of RSV which suppress cancer cell proliferation and induce apoptosis in the colon cancer cell line HT-29. RSV (150 uM) suppressed these pathways in the presence and absence of IGF-1, suggesting its role as a chemo-preventive agent even in obese condition. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5156757 Tue, 16 Aug 2011 23:00:00 +0100 5156757 http://www.medworm.com/index.php?rid=5138077&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F48 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5138077 Tue, 16 Aug 2011 23:00:00 +0100 5138077 http://www.medworm.com/index.php?rid=5117919&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F47 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5117919 Tue, 09 Aug 2011 23:00:00 +0100 5117919 http://www.medworm.com/index.php?rid=5094680&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F45 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5094680 Wed, 03 Aug 2011 23:00:00 +0100 5094680 http://www.medworm.com/index.php?rid=5094679&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F46 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5094679 Wed, 03 Aug 2011 23:00:00 +0100 5094679 http://www.medworm.com/index.php?rid=5085503&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F44 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5085503 Mon, 01 Aug 2011 23:00:00 +0100 5085503 http://www.medworm.com/index.php?rid=5076897&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F43 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5076897 Thu, 28 Jul 2011 23:00:00 +0100 5076897 http://www.medworm.com/index.php?rid=5076898&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F42 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5076898 Wed, 27 Jul 2011 23:00:00 +0100 5076898 http://www.medworm.com/index.php?rid=5068859&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F41 ${item.shortDescription} (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=5068859 Mon, 25 Jul 2011 23:00:00 +0100 5068859 http://www.medworm.com/index.php?rid=5035747&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F39 The present study focused on comparative proteome analyses of low- and high-temperature stresses and potential protein-protein interaction networks, constructed by using a bioinformatics approach, in response to both stress conditions.The data revealed two important points: first, the results indicate that low-temperature stress is tightly linked with oxidative stress as well as photosynthesis; however, no specific mechanism is revealed in the case of the high-temperature stress response. Second, temperature stress was revealed to be linked with nitrogen and ammonia assimilation. Moreover, the data also highlighted the cross-talk of signaling pathways. Some of the detected signaling proteins, e.g., Hik14, Hik26 and Hik28, have potential interactions with differentially expressed proteins i... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5035747 Thu, 14 Jul 2011 23:00:00 +0100 5035747 http://www.medworm.com/index.php?rid=5007153&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F38 Reversed phase high performance liquid chromatography (HPLC) interfaced to electrospray tandem mass spectrometry (MS/MS) is commonly used for the identification of peptides from proteolytically cleaved proteins embedded in a polyacrylamide gel matrix as well as for metabolomics screening. HPLC separations are time consuming (30-60 min average), costly (columns and mobile phase reagents), and carry the risk of column carry over between samples. The use of a chip-based nano-ESI platform (Advion NanoMate) based on replaceable nano-tips for sample introduction eliminates sample cross-contamination, provides unchanging sample matrix, and enhances spray stability with attendant increases in reproducibility. Recent papers have established direct infusion nano-ESI-MS/MS utilizing the NanoMate for ... Proteome Science journals http://www.medworm.com/rss/comments.php?id=5007153 Wed, 06 Jul 2011 23:00:00 +0100 5007153 http://www.medworm.com/index.php?rid=4992085&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F37 Conclusion: Heat stress induced the dephosphorylation of RuBisCo and the phosphorylation of ATP-beta, which decreased the activities of RuBisCo and ATP synthase. The observed dephosphorylation of the mRNA binding protein and 2-Cys peroxiredoxin may be involved in the transduction of heat-stress signaling, but the functional importance of other phosphoproteins, such as H+-ATPase, remains unknown. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4992085 Wed, 29 Jun 2011 23:00:00 +0100 4992085 http://www.medworm.com/index.php?rid=4975058&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F34 Background: A large variety of proteins involved in inflammation, coagulation, lipid-oxidation and lipid metabolism have been associated with high-density lipoprotein (HDL) and it is anticipated that changes in the HDL proteome have implications for the multiple functions of HDL. Here, SELDI-TOF mass spectrometry (MS) was used to study the dynamic changes of HDL protein composition in a human experimental low-dose endotoxemia model. Ten healthy men with low HDL cholesterol (0.7+/-0.1 mmol/L) and 10 men with high HDL cholesterol levels (1.9+/-0.4mmol/L) were challenged with endotoxin (LPS) intravenously (1ng/kg bodyweight). We previously showed that subjects with low HDL cholesterol are more susceptible to an inflammatory challenge. The current study tested the hypothesis that this discrepa... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4975058 Mon, 27 Jun 2011 23:00:00 +0100 4975058 http://www.medworm.com/index.php?rid=4975057&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F35 Conclusions: The data not only can extend our knowledge of canonical EGFR signaling, but also will be useful to understand the molecular mechanisms of EGFR in NPC pathogenesis and search therapeutic targets for NPC. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4975057 Mon, 27 Jun 2011 23:00:00 +0100 4975057 http://www.medworm.com/index.php?rid=4975056&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F36 Conclusions: 114 proteins were identified in supernatants from fresh cervical biopsies and many differed between CIN2 and 3. Cytokeratin 2 is the strongest discriminator with 90% overall cor-rect classifications. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4975056 Mon, 27 Jun 2011 23:00:00 +0100 4975056 http://www.medworm.com/index.php?rid=4975059&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F33 Conclusion: Taken together, presented proteomic data reveal that the constitutively altered cytokinin status of transgenic plants does not result in any qualitative changes in either stroma proteins or protein complexes of thylakoid membranes of fully developed chloroplasts, while few but significant quantitative differences are observed in stroma proteins. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4975059 Sat, 25 Jun 2011 23:00:00 +0100 4975059 http://www.medworm.com/index.php?rid=4967987&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F32 Conclusions: We identified a total of 42 immunoreactive proteins of the APP serotype 1 virulent strain S259 which represented 32 different proteins, including some novel immunoreactive factors which could be researched as vaccine candidates. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4967987 Sat, 25 Jun 2011 23:00:00 +0100 4967987 http://www.medworm.com/index.php?rid=4959535&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F31 Conclusions: We have identified a new gene family designated ECSA. Serum antibodies against the conserved domain of the ECSA family may be a promising tumor marker for esophageal SCC. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4959535 Wed, 22 Jun 2011 23:00:00 +0100 4959535 http://www.medworm.com/index.php?rid=4932975&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F30 Conclusions: This work provides guidance through the jungle of computational methods to analyze mass spectrometry-based isotope-labeled datasets and recommends an effective and easy-to-use evaluation strategy. We demonstrate our approach with three recently published datasets on Bacillus subtilis (Hahne et al. 2010, Otto et al. 2010) and Corynebacterium glutamicum (Haussmann et al. 2009). Special focus is placed on the application and validation of cluster analysis methods. All applied methods were implemented within the rich internet application QuPE (Albaum et al. 2009). Results can be found at http://qupe.cebitec.uni-bielefeld.de. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4932975 Fri, 10 Jun 2011 23:00:00 +0100 4932975 http://www.medworm.com/index.php?rid=4910820&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F28 Conclusion: These results could be used to better understand some aspects of the molecular mechanisms underlying porcine oocyte maturation. This study identified some regulatory proteins that may have important roles in the molecular events involved in porcine oocyte maturation, particularly with respect to the regulation of oocyte meiotic resumption, MII arrest and oocyte activation. In addition, this study may have beneficial applications not only to basic science with respect to the improvement of oocyte culture conditions but also to mammalian reproductive biotechnology with potential implications. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4910820 Tue, 07 Jun 2011 23:00:00 +0100 4910820 http://www.medworm.com/index.php?rid=4910819&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F29 Conclusions: In spite of some limitations mainly due to the extensive formalin-induced covalent cross-linking, our results essentially suggest the applicability of a proteomic approach to FFPE parathyroid specimens. From our point of view, FFPE extracts might be an alternative source,especially in the validation phase of protein biomarkers when a large cohort of samples is required and the low availability of frozen tissues might be constraining. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4910819 Tue, 07 Jun 2011 23:00:00 +0100 4910819 http://www.medworm.com/index.php?rid=4828358&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F26 Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4828358 Fri, 13 May 2011 23:00:00 +0100 4828358 http://www.medworm.com/index.php?rid=4820089&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F25 Conclusions: The obtained results indicate that at least two processes differentially contribute the plaque formation in CKD- and CVD-mediated atherosclerosis. It seems that the inflammatory process is more intense in CKD patients. On the other hand, the down- and up-regulation of apolipoprotein A-IV in CVD and CKD groups, respectively, suggests that substantial differences exist in the efficacy of cholesterol transport in both groups of patients. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4820089 Thu, 12 May 2011 23:00:00 +0100 4820089 http://www.medworm.com/index.php?rid=4801810&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F24 Conclusions: The immunoaffinity based kits exceeded the immobilized dye based kit in high abundance protein depletion of umbilical cord serum samples and dramatically improved 2DE gel quality for detection of trace biomarkers. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4801810 Sun, 08 May 2011 23:00:00 +0100 4801810 http://www.medworm.com/index.php?rid=4733157&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F23 Conclusions: A detergent extraction-based subunit vaccine of A. pleuropneumoniae was characterized by mass spectrometry. It contained a large variety of immunogenic and virulence associated proteins, among them the ApxIVA toxin. The identification of differences in expression as well as isoform variation between the serotypes implied the importance of combining proteins of different serotypes for vaccine generation. This finding was supported by immunoblotting showing the induction of cross-reactive antibodies against several surface associated proteins in immunized animals. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4733157 Tue, 19 Apr 2011 23:00:00 +0100 4733157 http://www.medworm.com/index.php?rid=4733158&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F22 Background: Proteomics may help us better understand the changes of multiple proteins involved in oncogenesis and progression of prostate cancer(PCa) and identify more diagnostic and prognostic biomarkers.The aim of this study was to screen biomarkers of PCa by the proteomics analysis using isobaric tags for relative and absolute quantification(iTRAQ). Methods: The patients undergoing prostate biopsies were classified into 3 groups according to pathological results: benign prostate hyperplasia (BPH, n=20), PCa(n=20) and BPH with local prostatic intraepithelial neoplasm(PIN, n=10). Then, all the specimens from these patients were analyzed by iTRAQ and two-dimensional liquid chromatography-tandem mass spectrometry (2DLC-MS/MS). The Gene Ontology(GO) function and the transcription regulation ... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4733158 Mon, 18 Apr 2011 23:00:00 +0100 4733158 http://www.medworm.com/index.php?rid=4723187&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F20 Conclusions: Inflammatory disease can severely interfere with the detection accuracy of SELDI profiles for lung cancer. Using a two-stage training process will improve the specificity and accuracy of detecting lung cancer. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4723187 Sat, 16 Apr 2011 23:00:00 +0100 4723187 http://www.medworm.com/index.php?rid=4723186&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F21 Conclusion: Taken together, our findings suggest Apo-A1 could be a potential biomarker related with early diagnosis and classification in two-tie grading system for bladder cancer. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4723186 Sat, 16 Apr 2011 23:00:00 +0100 4723186 http://www.medworm.com/index.php?rid=4692861&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F17 Conclusion: The present study demonstrated that this fractionation method could be applied for purposes of enriching low-abundance proteins and obtaining diversified fractionations of urine, and potentially other proteomes. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4692861 Thu, 07 Apr 2011 23:00:00 +0100 4692861 http://www.medworm.com/index.php?rid=4692860&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F18 Conclusions: We demonstrated here that sample deglycosylation improves the quantitative performance of shotgun proteomics, which can be effectively applied to any samples with high glycoprotein contents. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4692860 Thu, 07 Apr 2011 23:00:00 +0100 4692860 http://www.medworm.com/index.php?rid=4692859&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F19 Conclusions: The mass spectrometric immunoassays can find use in quantifying specific protein modifications, either as a part of a specific protein biomarker discovery/rediscovery effort to delineate the role of these variants in the onset of the disease, progression, and response to therapy, or in a more systematic study to delineate and understand human protein diversity. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4692859 Thu, 07 Apr 2011 23:00:00 +0100 4692859 http://www.medworm.com/index.php?rid=4683456&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F16 Conclusions: Our results confirmed the importance of previously described proteins in glioma pathology and their potential usefulness as biological markers but also revealed some new interesting targets for future therapies. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4683456 Tue, 05 Apr 2011 23:00:00 +0100 4683456 http://www.medworm.com/index.php?rid=4652470&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F14 The list of authors of this article [Miecznikowski JC, Damodaran S, Sellers KF, Rabin RA: A comparison of imputation procedures and statistical tests for the analysis of two-dimensional electrophoresis data. Proteome Science 2010, 8:66] was incorrect as published and should be as follows: Jeffrey C Miecznikowski, Senthilkumar Damodaran, Kimberly F Sellers, Donald E Coling, Richard Salvi, Richard A RabinCo-authors Donald E Coling and Richard Salvi were omitted in error from the published author list.JCM designed the study, performed the statistical analysis, and wrote the manuscript. SD designed the study, performed the data analysis, and wrote the manuscript. KFS assisted in the statistical analysis and the writing of the manuscript. DEC and RS provided the datasets for analysis. RAR provi... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4652470 Mon, 28 Mar 2011 23:00:00 +0100 4652470 http://www.medworm.com/index.php?rid=4652469&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F15 Conclusion: Our unbiased systems analysis has led to the potential discovery of unexpected functions in normal endothelium. The discovery of the existence of protein synthesis at the plasma membrane in endothelial cells provides new insight into the blood-tissue interface and endothelial cell surface biology. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4652469 Mon, 28 Mar 2011 23:00:00 +0100 4652469 http://www.medworm.com/index.php?rid=4621775&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F13 Conclusions: We conclude that MALDI-TOF MS of urine does not reveal a clear hyperactive delirium proteome fingerprint in ICU patients.Clinical Trial Register number: NCT00604773 (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4621775 Tue, 22 Mar 2011 00:00:00 +0100 4621775 http://www.medworm.com/index.php?rid=4565610&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F12 Conclusion: The present study implicated the particular role of the elements of DNA repair, post-tanscriptional regulation and heterotrimeric G protein signaling in response to Pb (II) stress as shown for the first time for a basidiomycete. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4565610 Wed, 09 Mar 2011 00:00:00 +0100 4565610 http://www.medworm.com/index.php?rid=4559707&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F11 Conclusions: To the best of our knowledge, we have performed the first analysis of the proteomic changes in chicken embryonic trachea and kidney tissues during IBV infection in ovo. The data obtained should facilitate a better understanding of the pathogenesis of IBV infection. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4559707 Tue, 08 Mar 2011 00:00:00 +0100 4559707 http://www.medworm.com/index.php?rid=4464210&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F10 Conclusions: This is the first successful effort to identify the majority of abundant flour proteins for a single wheat cultivar, relate them to individual gene sequences and estimate their relative levels. Many genes for wheat flour proteins are not expressed, so this study represents further progress in describing the expressed wheat genome. Use of cultivar-specific contigs helped to overcome the difficulties of matching peptides to gene sequences for members of highly similar, rapidly evolving storage protein families. Prospects for simplifying this process for routine analyses are discussed. The ability to measure expression levels for individual flour protein genes complements information gained from efforts to sequence the wheat genome and is essential for studies of effects of envir... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4464210 Fri, 11 Feb 2011 00:00:00 +0100 4464210 http://www.medworm.com/index.php?rid=4454947&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F8 Conclusions: Our results imply that a mechanism of protein release into AH after surgery is a global response to the surgery rather than increase in amount of protective proteins found in healthy AH and a mechanism of protein release for each type of incision procedure could be different. Although the total protein concentration was increased (at 0.5 and 2 hour time points and between types of surgery) many of 2-DE protein spots were similar based on 2-DE and MS analyses, and only a small number of protein spots changed with either the time points or surgical conditions (0.4 -1.9%). This suggests that the high protein content is due to an increase in the concentration of the same proteins with only a few unique proteins being altered per time point and with the different surgery type. This... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4454947 Wed, 09 Feb 2011 00:00:00 +0100 4454947 http://www.medworm.com/index.php?rid=4445455&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F6 Conclusion: Despite the presence of confounding factors, the disease specific changes in abundance exhibited by a panel of urine proteins permitted the creation of classifiers able to discriminate between control and infected cattle with a high degree of accuracy. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4445455 Mon, 07 Feb 2011 00:00:00 +0100 4445455 http://www.medworm.com/index.php?rid=4436487&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F5 Background: Hepatitis E is endemic to resource-poor regions, where it manifests as sporadic cases and large waterborne outbreaks. The disease severity ranges from acute self-limited hepatitis with low mortality to fulminant hepatic failure with high mortality. It is believed that the host response plays an important role in determining the progression and outcome of this disease. We profiled the plasma peptidome from hepatitis E patients to discover suitable biomarkers and understand disease pathogenesis. Results: The peptidome ( (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4436487 Fri, 04 Feb 2011 00:00:00 +0100 4436487 http://www.medworm.com/index.php?rid=4377185&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F4 Conclusions: Because the majority of the nonmucin proteins possess molecular weights less than that selected for analysis, it is theoretically possible that they may associate with the high molecular weight and negatively charged mucins to form a highly ordered structural organization that is likely to be important for maintaining the proper defensive function of airway mucus. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4377185 Thu, 20 Jan 2011 00:00:00 +0100 4377185 http://www.medworm.com/index.php?rid=4371035&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F3 Conclusions/ Significance:In conclusion, our CD34+/K15+ HBPCs could be induced to transdifferentiate into cardiomyocyte-like cells using a small molecule called Cardiogenol C. The process involves activation of the Wnt signaling pathway and altered expression of several key chromatin remodeling proteins. The finding is clinically significant as HBPCs offer a readily accessible and autologous source of progenitor cells for cell-based therapy of heart disease, which is one of major killers in developed countries. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4371035 Wed, 19 Jan 2011 00:00:00 +0100 4371035 http://www.medworm.com/index.php?rid=4360525&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F2 We describe a method to identify metabolites of exogenous proteins that eliminates endogenous background by using stable isotope labeled matrices. This technique allows selective screening of the intact therapeutic molecule and all metabolites using a modified precursor ion scan that monitors low molecular weight fragment ions produced during MS/MS. This distinct set of low mass ions differs between isotopically labeled and natural isotope containing species allowing excellent discrimination between endogenous compounds and target analytes during the precursor scanning experiments. All compounds containing amino acids that consist of naturally abundant isotopes can be selected using this scanning technique for further analysis, including metabolites of the parent molecule. The sensitivity ... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4360525 Mon, 17 Jan 2011 00:00:00 +0100 4360525 http://www.medworm.com/index.php?rid=4330557&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F9%2F1%2F1 Conclusion: The data obtained in this study support the hypothesis that isoform-specific effects of CRP may differentially regulate the phenotype of monocytes. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4330557 Mon, 10 Jan 2011 00:00:00 +0100 4330557 http://www.medworm.com/index.php?rid=4300402&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F70 Hepatocellular carcinoma (HCC) is the fifth most common cancer and advanced hepatic fibrosis is a major risk factor for HCC. Hepatic fibrosis including liver cirrhosis and HCC are mainly induced by persistent hepatitis B or C virus infection, with approximately 500 million people infected with hepatitis B or C virus worldwide. Furthermore, the number of patients with non-alcoholic fatty liver disease (NAFLD) has recently increased and NAFLD can progress to cirrhosis and HCC. These chronic liver diseases are major causes of morbidity and mortality, and the identification of non-invasive biomarkers is important for early diagnosis. Recent advancements in quantitative and large-scale proteomic methods could be used to optimize the clinical application of biomarkers. Early diagnosis of HCC and... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4300402 Fri, 31 Dec 2010 00:00:00 +0100 4300402 http://www.medworm.com/index.php?rid=4284885&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F69 Conclusions: RNAi is a valuable tool for the identification of very specific and highly sensitive antibodies, and is therefore especially useful for the validation of RPPA-suitable detection antibodies. On the other hand, when a set of well-characterized RPPA-antibodies is available, large-scale RNAi experiments analyzed by RPPA might deliver useful information for network reconstruction. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4284885 Thu, 23 Dec 2010 00:00:00 +0100 4284885 http://www.medworm.com/index.php?rid=4264885&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F67 Conclusion: Taken together, these findings suggest that PGM3 plays a role in mediating SFN-induced cell death in LNCaP cells, and is a potential molecular therapeutic target for prostate cancer. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4264885 Thu, 16 Dec 2010 00:00:00 +0100 4264885 http://www.medworm.com/index.php?rid=4264884&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F68 Conclusion: Our data supports the notion of the complementary use of dMS and SC for label-free comparative proteomics, broadening the identification spectrum and strengthening the identification of trends in protein expression changes during the particular processes being compared. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4264884 Thu, 16 Dec 2010 00:00:00 +0100 4264884 http://www.medworm.com/index.php?rid=4264887&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F65 Conclusion: This proteomic analysis provides evidence that other salivary polypeptides, in addition to tannin-precipitating proteins, are affected by tannin ingestion. Changes in the expression levels of the acidic mammalian chitinase precursor and one of 14 salivary amylase isoforms underscores the need to further investigate their role in tannin ingestion. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4264887 Wed, 15 Dec 2010 00:00:00 +0100 4264887 http://www.medworm.com/index.php?rid=4264886&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F66 Conclusions: In summary, we advocate for a three-step statistical analysis of two-dimensional gel electrophoresis (2-DE) data with a data imputation step, choice of statistical test, and lastly an error control method in light of multiple testing. When determining the choice of statistical test, it is worth considering whether the protein spots will be subjected to mass spectrometry. If this is the case a more liberal test such as the percentile-based bootstrap t can be employed. For error control in electrophoresis experiments, we advocate that gFWER be controlled for multiple testing rather than the false discovery rate. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4264886 Wed, 15 Dec 2010 00:00:00 +0100 4264886 http://www.medworm.com/index.php?rid=4248724&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F64 Conclusion: Our results indicate a positive correlation between the gene and protein expression patterns of SsOEE2 and the rejuvenation process, suggesting that this gene is involved in the rejuvenation of Sequoia sempervirens. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4248724 Fri, 10 Dec 2010 00:00:00 +0100 4248724 http://www.medworm.com/index.php?rid=4241418&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F63 Cervicovaginal fluid has an important function in the homeostasis and immunity of the lower female genital tract. Analysis of the cervicovaginal fluid proteome may therefore yield important information about the pathogenesis of numerous gynecological pathologies. Additionally, cervicovaginal fluid has great potential as a source of biomarkers for these conditions. This review provides a detailed discussion about the human cervicovaginal proteome and the proteomics studies performed to characterize this biological fluid. Furthermore, infection-correlated pathological conditions of the female genital tract are discussed for which cervicovaginal fluid has been used in order to identify potential biomarkers. Recent years, numerous studies have analyzed cervicovaginal fluid samples utilizing an... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4241418 Wed, 08 Dec 2010 00:00:00 +0100 4241418 http://www.medworm.com/index.php?rid=4219151&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F62 Conclusion: This simple method can be applied easily to common high-abundance protein depletion methods, effectively reducing experimental variations in biomarker discovery studies. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4219151 Wed, 01 Dec 2010 00:00:00 +0100 4219151 http://www.medworm.com/index.php?rid=4189713&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F60 Conclusions: The QSRR-based model for predicting retention times of the peptides, was a useful additional to MS tool, enhancing protein identification. Higher content of SASPs in strain lacking PrpE phosphatase suggests that this enzyme may influence their occurrence in the spores, lowering levels of these proteins. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4189713 Mon, 22 Nov 2010 00:00:00 +0100 4189713 http://www.medworm.com/index.php?rid=4189712&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F61 Conclusion: Stabilization at the time of collection offers the potential to better preserve tissue protein and protein modification levels, as well as reduce the variability related to tissue processing delays that are often associated with clinical samples. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4189712 Mon, 22 Nov 2010 00:00:00 +0100 4189712 http://www.medworm.com/index.php?rid=4175306&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F58 Conclusion: CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4175306 Wed, 17 Nov 2010 00:00:00 +0100 4175306 http://www.medworm.com/index.php?rid=4167298&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F57 Conclusions: Elaboration of a reference proteome constitutes the first step in creating a gene expression database dedicated to capillary endothelial cells displaying BBB characteristics. It improves of our knowledge of the BBB and the key proteins in cell structures, cytoskeleton organization, metabolism, detoxification and drug resistance. Moreover, our results emphasize the need for both appropriate experimental design and correct interpretation of proteome datasets. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4167298 Mon, 15 Nov 2010 00:00:00 +0100 4167298 http://www.medworm.com/index.php?rid=4150354&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F55 Conclusions: This is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of c... Proteome Science journals http://www.medworm.com/rss/comments.php?id=4150354 Tue, 09 Nov 2010 00:00:00 +0100 4150354 http://www.medworm.com/index.php?rid=4132923&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F54 Conclusion: This is one of the first comprehensive proteomic study of a molluscan shell, and should provide a platform for further characterization of matrix protein functions and interactions. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4132923 Thu, 04 Nov 2010 00:00:00 +0100 4132923 http://www.medworm.com/index.php?rid=4085907&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F53 Conclusions: We identified the presence of CRMP-2-deltaC in the brain of a murine model of prion disease. Of note, C-terminal truncations of CRMP-2 have been recently observed in models for neurodegenerative disorders such as ischemia, traumatic brain injury, and Wallerian degeneration. While the structural identity of CRMP-2-deltaC in those models remains unknown, the present study should provide clues to the molecular pathology of degenerating neurons in prion diseases in connection with other neurodegenerative disorders. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4085907 Tue, 19 Oct 2010 23:00:00 +0100 4085907 http://www.medworm.com/index.php?rid=4076826&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F52 Conclusions Exposure of primary human endothelial cells to the mobile phone radiation, 1800 MHz GSM signal for 1 hour at an average specific absorption rate of 2.0 W/kg, does not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with our earlier study showing that the 1800 MHz GSM radiation exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926, as compared with the effect of 900 MHz GSM radiation. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4076826 Sun, 17 Oct 2010 23:00:00 +0100 4076826 http://www.medworm.com/index.php?rid=4060023&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F50 Conclusions: Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might be causing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of infection by Gram-negative bacteria within farming plants. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=4060023 Mon, 11 Oct 2010 23:00:00 +0100 4060023 http://www.medworm.com/index.php?rid=3993077&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F49 Conclusions: Taken together, our data suggest that altered protein expression, structural protein remodeling, and the reduction of translation might play important roles in the early response to oxygen deprivation in C. elegans, and this information will help broaden our knowledge on the mechanism of hypoxia response. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3993077 Mon, 20 Sep 2010 23:00:00 +0100 3993077 http://www.medworm.com/index.php?rid=3986568&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F48 Conclusions: The comprehensive description of proteins isolated from vancomycin-resistant Enterococcus faecium and E. durans may provide new targets for development of antimicrobial agents. This knowledge may help to identify new biomarkers of antibiotic resistance and virulence factors. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3986568 Mon, 20 Sep 2010 23:00:00 +0100 3986568 http://www.medworm.com/index.php?rid=3968733&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F47 Conclusions: Proteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3968733 Mon, 13 Sep 2010 23:00:00 +0100 3968733 http://www.medworm.com/index.php?rid=3953542&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F46 Conclusions: This study is the first report on proteomics on D. seriata. The proteomic data obtained will be important to understand the pathogenicity process. In fact, several of the identified proteins have been reported as pathogenicity factors in other phytopathogenic fungi. Moreover, this proteomic analysis supposes a useful basis for deepening into D. seriata knowledge and will contribute to the development of the molecular biology of this fungal strain as it has been demonstrated by cloning the gene Prx1 encoding mitochondrial peroxiredoxin-1 of D. seriata (the first gene to be cloned in this microorganism; data not shown). (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3953542 Wed, 08 Sep 2010 23:00:00 +0100 3953542 http://www.medworm.com/index.php?rid=3861655&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F44 Background: Growth cessation, cold acclimation, and dormancy induction in grapevines and other woody perennial plants native to temperate continental climates is frequently triggered by short photoperiods. The early induction of these processes by photoperiod promotes winter survival of grapevines in cold temperate zones. Examining the molecular processes, in particular the proteomic changes in the shoot, will provide greater insight into the signaling cascade that initiates growth cessation and dormancy induction. To begin understanding transduction of the photoperiod signal, Vitis riparia Michx. grapevines that had grown for 35 days in long photoperiod (long day, LD, 15h) were subjected to either a continued LD or a short photoperiod (short day, SD, 13h) treatment. Shoot tips (4-node sho... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3861655 Wed, 11 Aug 2010 23:00:00 +0100 3861655 http://www.medworm.com/index.php?rid=3857898&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F43 Conclusion: These results showed that DIGE saturation labeling is a powerful technology for proteomics studies in small number of ticks and provided new information about the effect of pathogen infection in ticks. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3857898 Wed, 11 Aug 2010 23:00:00 +0100 3857898 http://www.medworm.com/index.php?rid=3802048&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F42 Conclusion: Overall, the results from our study indicate that both plasma and serum samples are equally suited for proteomic studies, and provide similar results. These findings are particularly important for studies profiling proteomic differences in lipoprotein particle composition in a variety of disease conditions, including cardiovascular disease. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3802048 Wed, 28 Jul 2010 23:00:00 +0100 3802048 http://www.medworm.com/index.php?rid=3757516&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F40 Conclusion: We conclude it is likely that such studies on museum specimens using a proteomic approach will be limited in coverage of proteins and may, therefore, be misleading. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3757516 Tue, 13 Jul 2010 23:00:00 +0100 3757516 http://www.medworm.com/index.php?rid=3749829&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F39 Conclusions: This work suggests the usefulness of a proteomic approach to the study of shrimp immunity and revealed hemocyte proteins whose expression were up regulated upon V. harveyi infection such as hemocyanin, arginine kinase and down regulated such as alpha-2-macroglobulin, calmodulin and 14-3-3 protein epsilon. The information is useful for understanding the immune system of shrimp against pathogenic bacteria. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3749829 Mon, 12 Jul 2010 23:00:00 +0100 3749829 http://www.medworm.com/index.php?rid=3740320&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F38 Background: During and following myocardial ischemia, glucose oxidation rates are low and fatty acids dominate as a source of oxidative metabolism. This metabolic phenotype is associated with contractile dysfunction during reperfusion. To determine the mechanism of this reliance on fatty acid oxidation as a source of ATP generation, a functional proteomics approach was utilized. Results: 2-D gel electrophoresis of mitochondria from working rat hearts subjected to 25 minutes of global no flow ischemia followed by 40 minutes of aerobic reperfusion identified 32 changes in protein abundance compared to aerobic controls. Of the five proteins with the greatest change in abundance, two were increased (long chain acyl-coenzyme A dehydrogenase (481 versus 393 arbitrary units, n=3, P (Source: Prote... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3740320 Thu, 08 Jul 2010 23:00:00 +0100 3740320 http://www.medworm.com/index.php?rid=3699185&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F37 Conclusion: The results provide an overview of potentially important proteins that E. faecalis V583 needs to regulate in order to survive and adapt to a bile-rich environment, among which are several proteins involved in fatty acid and phospholipid biosynthesis pathways. In addition, this study reveals several hypothetical proteins, which are both abundant and clearly regulated and thus stand out as targets for future studies on bile stress. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3699185 Thu, 24 Jun 2010 23:00:00 +0100 3699185 http://www.medworm.com/index.php?rid=3686837&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F36 Conclusions: Antibody-mediated signal amplification is a convenient and cost-effective approach for the robust and specific quantification of low abundant proteins on RPPAs. Contrasting other amplification approaches it allows target protein detection over a large linear range. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3686837 Mon, 21 Jun 2010 23:00:00 +0100 3686837 http://www.medworm.com/index.php?rid=3671243&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F33 Conclusions: The spicule matrix is a relatively complex mixture of proteins not only containing matrix-specific proteins with a function in matrix assembly or mineralization, but also: 1) proteins possibly important for the formation of the continuous membrane delineating the mineralization space; 2) proteins for secretory processes delivering proteinaceous or non-proteinaceous precursors; 3) or proteins reflecting signaling events at the cell/matrix interface. Comparison of the proteomes of different skeletal matrices allows prediction of proteins of general importance for mineralization in sea urchins, such as SM50, SM30-E, SM29 or MSP130. The comparisons also help point out putative tissue-specific proteins, such as tooth phosphodontin or specific spicule matrix metalloproteases of the ... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3671243 Wed, 16 Jun 2010 23:00:00 +0100 3671243 http://www.medworm.com/index.php?rid=3671242&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F34 Conclusions: These indicate that SP-A plays a key role in regulating the BAL proteome, functioning indirectly to regulate lung host defense function, possibly via the macrophage. In the absence of SP-A baseline levels of many host defense molecules are lower. However, many of these indirect deficits in SP-A-/- mice are rapidly compensated for during infection, indicating that SP-A also has a direct role on host defense against K. pneumoniae that may be instrumental in determining clinical course. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3671242 Wed, 16 Jun 2010 23:00:00 +0100 3671242 http://www.medworm.com/index.php?rid=3662338&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F32 Conclusion: The results are consistent with the hypothesis that the wheat germ extract enhances the protein folding capabilities of the in vitro system by providing eukaryotic ribosomes and chaperones and, at the same time, the E. coli S30 extract, which includes an ATP regeneration system, translates the polypeptides at high rates. This hybrid cell-free expression system allows the facile production of high-yield protein arrays suitable for downstream assays. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3662338 Mon, 14 Jun 2010 23:00:00 +0100 3662338 http://www.medworm.com/index.php?rid=3662339&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F31 Conclusions: This study provides the first analysis of immunodepleted serum in combination with iTRAQ to measure relative protein expression in ovarian cancer patients for the pursuit of serum biomarkers. Several candidate biomarkers were identified which warrant further development. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3662339 Sun, 13 Jun 2010 23:00:00 +0100 3662339 http://www.medworm.com/index.php?rid=3644494&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F29 Conclusions: The results present the first standard proteomic profile of IBV and may facilitate the understanding of the pathogenic mechanisms. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3644494 Tue, 08 Jun 2010 23:00:00 +0100 3644494 http://www.medworm.com/index.php?rid=3635487&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F28 Conclusions: Differentially expressed proteins of DHBV infected PDHs revealed by 2-DE, are involved in carbohydrate metabolism, amino acid metabolism, stress responses and cytoskeleton processes etc, providing the insight to understanding of interactions between hepadnavirus and hepatocytes and molecular mechanisms of hepadnavirus pathogenesis. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3635487 Sun, 06 Jun 2010 23:00:00 +0100 3635487 http://www.medworm.com/index.php?rid=3548998&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F24 Conclusions: Our study demonstrated that identification of proteins within minute amounts of sample material improved significantly by combining sensitive LC-MS methods with different de novo peptide sequencing algorithms. In addition, this is the first study that verified protein prediction from MS data by also employing a second complementary approach, in which RACE-PCR led to identification of a novel elicitor protein in P. halstedii. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3548998 Sun, 09 May 2010 23:00:00 +0100 3548998 http://www.medworm.com/index.php?rid=3511204&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F23 Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gel-free proteomics. Based on two independent biochemical characteristics of proteins, two-dimensional electrophoresis combines isoelectric focusing, which separates proteins according to their isoelectric point, and SDS-PAGE, which separates them further according to their molecular mass. The next typical steps of the flow of gel-based proteomics are spots visualization and evaluation, expression analysis and finally protein identification by mass spectrometry. For the study of differentially expressed proteins, two-dimensional electrophoresis allows simultaneousl... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3511204 Tue, 27 Apr 2010 23:00:00 +0100 3511204 http://www.medworm.com/index.php?rid=3463285&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F22 Conclusions. This study reveals several novel protein partners for PARP-1, PARP-2 and PARG. It provides a global view of the interactomes of these proteins as well as a roadmap to establish the systems biology of poly(ADP-ribose) metabolism. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3463285 Mon, 12 Apr 2010 23:00:00 +0100 3463285 http://www.medworm.com/index.php?rid=3453362&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F21 Mycobacterium avium subsp. paratuberculosis is a pathogen which causes a debilitating chronic enteritis in ruminants. Unfortunately, the mechanisms that control M. avium subsp. paratuberculosis persistence during infection are poorly understood and the key steps for developing Johne's disease remain elusive. A proteomic analysis approach, based on one dimensional polyacrylamide gel electrophoresis (SDS-PAGE) followed by LC-MS/MS, was used to identify and characterize the cell wall associated proteins of M. avium subsp. paratuberculosis K10 and an cell surface enzymatic shaving method was used to determine the surface-exposed proteins. 309 different proteins were identified, which included 101 proteins previously annotated as hypothetical or conserved hypothetical. The cell surface enzymati... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3453362 Wed, 07 Apr 2010 23:00:00 +0100 3453362 http://www.medworm.com/index.php?rid=3415665&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F19 Conclusion: These results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3415665 Sun, 28 Mar 2010 23:00:00 +0100 3415665 http://www.medworm.com/index.php?rid=3407916&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F18 Conclusion: Our work represents an essential step in the knowledge of the molecular properties of WJCs to better understand their possible use in the field of cell therapy and regenerative medicine. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3407916 Fri, 26 Mar 2010 00:00:00 +0100 3407916 http://www.medworm.com/index.php?rid=3403469&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F16 Conclusions: The SELDI-TOF-MS is a useful tool to help diagnose colorectal cancer, especially during the early stage. However, identification of the significantly differentiated proteins needs further study. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3403469 Thu, 25 Mar 2010 00:00:00 +0100 3403469 http://www.medworm.com/index.php?rid=3377987&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F14 Conclusion: The increased expression of annexin 1 protein has been found to be associated with higher BF thickness in both crossbred steers. This result lays the foundation for future studies to develop the protein marker for assessing animals with different BF. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3377987 Thu, 18 Mar 2010 00:00:00 +0100 3377987 http://www.medworm.com/index.php?rid=3358573&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F12 Conclusions: Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3358573 Fri, 12 Mar 2010 00:00:00 +0100 3358573 http://www.medworm.com/index.php?rid=3326589&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F11 Conclusions: The results suggest that this study provides a potential link between the alterations in RBC membrane proteome in AD subjects and AD pathology. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3326589 Wed, 03 Mar 2010 00:00:00 +0100 3326589 http://www.medworm.com/index.php?rid=3311231&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F9 Conclusion: The method is feasible and reproducible. We could obtain a fair enrichment of cells but the major benefit of the method was an effective removal of contaminants from red blood cells and plasma proteins resulting in larger proteome coverage compared to the direct lysis of frozen samples. This sample preparation method may be successfully implemented for the discovery of lung cancer biomarkers on tissue samples using mass spectrometry-based proteomics. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3311231 Fri, 26 Feb 2010 00:00:00 +0100 3311231 http://www.medworm.com/index.php?rid=3275527&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F8 Conclusions: Permutations of C- and N-terminal Y2H vectors dramatically increase the coverage of interactome studies and thus significantly reduce the number of false negatives. We suggest that future interaction screens should use such vector combinations on a routine basis, not the least because they provide a built-in quality score for Y2H interactions that can provide a measure of reproducibility without additional assays. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3275527 Mon, 15 Feb 2010 00:00:00 +0100 3275527 http://www.medworm.com/index.php?rid=3254110&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F7 The yeast-like epiphytic fungus Pseudozyma flocculosa is known to antagonize powdery mildew fungi through proliferation on colonies presumably preceded by the release of an antifungal glycolipid (flocculosin). In culture conditions, P. flocculosa can be induced to produce or not flocculosin through manipulation of the culture medium nutrients. In order to characterize and understand the metabolic changes in P. flocculosa linked to glycolipid production, we conducted a 2-DE proteomic analysis and compared the proteomic profile of P. flocculosa growing under conditions favoring the development of the fungus (control) or conducive to flocculosin synthesis (stress). A large number of protein spots (771) were detected in protein extracts of the control treatment compared to only 435 matched pro... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3254110 Tue, 09 Feb 2010 00:00:00 +0100 3254110 http://www.medworm.com/index.php?rid=3250943&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F6 Conclusions: The 64 phosphorylation sites determined represent the most comprehensive list of experimentally identified sea urchin protein phosphorylation sites at present and are an important addition to the recently analyzed Strongylocentrotus purpuratus shell and tooth proteomes. The identified phosphoproteins included a major, highly phosphorylated protein, [Glean3:18919], for which we suggest the name phosphodontin. Although not sequence-related to such highly phosphorylated acidic mammalian dental phosphoproteins as phosphoryn or dentin matrix protein-1, phosphodontin may perform similar functions in the sea urchin tooth. More than half of the detected proteins were not previously identified at the protein level, thus confirming the existence of proteins only known as genomic sequenc... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3250943 Mon, 08 Feb 2010 00:00:00 +0100 3250943 http://www.medworm.com/index.php?rid=3243329&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F5 Conclusions: These findings demonstrate the practicality and versatility of non-directed activity-based protein profiling (ABPP) to complement directed methods and accelerate the discovery of altered protein activities associated with pathological states such as HCV replication. Collectively, these results highlight the ability of in situ ABPP approaches to facilitate the identification of enzymes that are either predominantly or exclusively labeled in living cells. Several of these differentially active enzymes represent possible HCV-host interactions that could be targeted for diagnostic or therapeutic purposes. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3243329 Thu, 04 Feb 2010 00:00:00 +0100 3243329 http://www.medworm.com/index.php?rid=3239203&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F4 Conclusion: These results demonstrate the capacity of proteomic analysis of plasma, by confirming the presence of known biomarkers as well as revealing new biomarkers for diabetic nephropathy in plasma in type 1 diabetic patients. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3239203 Wed, 03 Feb 2010 00:00:00 +0100 3239203 http://www.medworm.com/index.php?rid=3220117&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F2 Conclusions: This manuscript described a more comprehensive proteomic profile of prolactinomas compared to other previous published reports. Thanks to the application of immuno-LCM combined with online two-dimensional nano-scale liquid chromatography here permitted identification of more proteins and, to our best knowledge, generated the largest prolactinoma proteome. This enlarged proteome would contribute significantly to further understanding of prolactinoma tumorigenesis which is crucial to the management of prolactinomas. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3220117 Fri, 29 Jan 2010 00:00:00 +0100 3220117 http://www.medworm.com/index.php?rid=3220116&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F3 Conclusion: Administration of doxycycline might be a significant supportive therapeutic strategy in prevention of VILI. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3220116 Fri, 29 Jan 2010 00:00:00 +0100 3220116 http://www.medworm.com/index.php?rid=3166756&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F8%2F1%2F1 Conclusions: Many proteins identified in our study corresponded to the proteins identified in other insects. Compared to the former studies performed in insects, we presented 2-D protein maps of the hemocytes and fat bodies and showed the trends in expression of the immune-elicited proteins. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3166756 Wed, 13 Jan 2010 00:00:00 +0100 3166756 http://www.medworm.com/index.php?rid=3126456&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F48 Conclusions: Our study provides novel evidence for mitochondria as a pivotal organelle that is connected to liver regeneration, and lays the foundation for further studies on key factors and pathways involved in liver regeneration following 50% PH, a condition frequently used for partial liver transplantation and conservative liver resection. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3126456 Tue, 29 Dec 2009 00:00:00 +0100 3126456 http://www.medworm.com/index.php?rid=3110913&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F45 Conclusions: Our results indicate the physiological differences between the non-pathogenic strain, with its non-infective proliferate lifestyle, and the pathogenic strains, with its constitutive expression of adhesins, which would render the bacterium competent for adhesion and infection prior to host contact. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3110913 Mon, 21 Dec 2009 00:00:00 +0100 3110913 http://www.medworm.com/index.php?rid=3084535&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F44 Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=3084535 Mon, 14 Dec 2009 00:00:00 +0100 3084535 http://www.medworm.com/index.php?rid=3018706&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F43 Background: B-cell lymphomas are thought to reflect different stages of B-cell maturation. Based on cytogenetics and molecular markers, mantle cell lymphoma (MCL) is presumed to derive predominantly from naive, pre-germinal centre (pre-GC) B lymphocytes. The aim of this study was to develop a method to investigate the similarity between MCL and different B-cell compartments on a protein expression level. Methods: Subpopulations of B cells representing the germinal centre (GC), the pre-GC mantle zone and the post-GC marginal zone were isolated from tonsils using automated magnetic cell sorting (AutoMACS) of cells based on their expression of CD27 and IgD. Protein profiling of the B cell subsets, of cell lines representing different lymphomas and of primary mantle cell lymphoma (MCL) samples... Proteome Science journals http://www.medworm.com/rss/comments.php?id=3018706 Mon, 23 Nov 2009 00:00:00 +0100 3018706 http://www.medworm.com/index.php?rid=2976992&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F42 Conclusion: In this study, we successfully identified novel salt stress-responsive protein phosphorylation sites from membrane isolates of abiotic-stressed plants by membrane shaving followed by Zr4+-IMAC enrichment. The identified phosphorylation sites can be important in the salt stress response in plants. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2976992 Tue, 10 Nov 2009 00:00:00 +0100 2976992 http://www.medworm.com/index.php?rid=2962881&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F41 Conclusion: The aqueous polymer two-phase partition is a simple, rapid and relatively inexpensive method. It is well suitable for the purification of PMs from small amount of tissues. Therefore, it is reasonable for the DRG PM to be enriched by using aqueous two-phase partition as a preferred method. Proteomic analysis showed that DRG PM was rich in proteins involved in the fundamental biological processes including material exchange, energy transformation and information transmission, etc. These data would help to our further understanding of the fundamental DRG functions. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2962881 Thu, 05 Nov 2009 00:00:00 +0100 2962881 http://www.medworm.com/index.php?rid=2950421&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F40 Conclusion: Using a proteomic approach we identified biomarkers related to the lipoprotein profile that could be used to predict growth response to GH treatment in prepubertal children who are short as a result of GH-deficiency or who have ISS.These results support our previous findings that apolipoproteins and transthyretin may have a role in GH sensitivity. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2950421 Mon, 02 Nov 2009 00:00:00 +0100 2950421 http://www.medworm.com/index.php?rid=2934406&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F39 Background: Hepatitis E, caused by the hepatitis E virus (HEV), is endemic to developing countries where it manifests as waterborne outbreaks and sporadic cases. Though generally self-limited with a low mortality rate, some cases progress to fulminant hepatic failure (FHF) with high mortality. With no identified predictive or diagnostic markers, the events leading to disease exacerbation are not known. Our aim is to use proteomic tools to identify biomarkers of acute and fulminant hepatitis E. Results: We analyzed proteins in the plasma and urine of hepatitis E patients and healthy controls by two-dimensional Differential Imaging Gel Electrophoresis (DIGE) and mass spectrometry, and identified over 30 proteins to be differentially expressed during acute hepatitis E. The levels of one plasm... Proteome Science journals http://www.medworm.com/rss/comments.php?id=2934406 Tue, 27 Oct 2009 00:00:00 +0100 2934406 http://www.medworm.com/index.php?rid=2814281&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F36 In this study a proteomic approach was used to investigate the steady-state response of Escherichia coli to temperature up-shifts in a cascade of two continuously operated bioreactors. The first reactor served as cell source with optimal settings for microbial growth, while in the second chemostat the cells were exposed to elevated temperatures. By using this reactor configuration, which has not been reported to be used for the study of bacterial stress responses so far, it is possible to study temperature stress under well-defined, steady-state conditions. Specifically the effect on the cellular adaption to temperature stress using two-dimensional gel electrophoresis was examined and compared at the cultivation temperatures of 37degreesC and 47.5degreesC. As expected, the steady-state stu... Proteome Science journals http://www.medworm.com/rss/comments.php?id=2814281 Sun, 20 Sep 2009 23:00:00 +0100 2814281 http://www.medworm.com/index.php?rid=2765625&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F34 Conclusions: This study shows that serum peptidome profiling using MALDI-TOF-MS coupled to pattern diagnostics may aid in prediction of treatment outcome of advanced NSCLC patients treated with chemotherapy. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2765625 Wed, 02 Sep 2009 23:00:00 +0100 2765625 http://www.medworm.com/index.php?rid=2757308&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F33 The present study examined the changes in protein expression in Spirulina platensis upon exposure to high temperature, with the changes in expression analyzed at the subcellular level. In addition, the transcriptional expression level of some differentially expressed proteins, the expression pattern clustering, and the protein-protein interaction network were analyzed. The results obtained from differential expression analysis revealed up-regulation of proteins involved in two-component response systems, DNA damage and repair systems, molecular chaperones, known stress-related proteins, and proteins involved in other biological processes, such as capsule formation and unsaturated fatty acid biosynthesis. The clustering of all differentially expressed proteins in the three cellular compartm... Proteome Science journals http://www.medworm.com/rss/comments.php?id=2757308 Tue, 01 Sep 2009 23:00:00 +0100 2757308 http://www.medworm.com/index.php?rid=2751412&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F32 Conclusions: The present report provides new information about the protein content of human neutrophils. Importantly, our study resulted in the discovery of a series of proteins not previously reported to be associated with human neutrophils. These data are relevant to the investigation of comparative pathological states and models for novel classes of pharmaceutical drugs that could be useful in the treatment of inflammatory disorders in which neutrophils participate. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2751412 Sun, 30 Aug 2009 23:00:00 +0100 2751412 http://www.medworm.com/index.php?rid=2743797&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F31 Conclusions: This set of experiments establishes a new model system for genetic dissection of the specific HSA21 gene-overdose contributions to aberrant cell migration, adhesion, proliferation and specific proteome response to collagen VI, cellular phenotypes linked to the pathogenesis of CHD. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2743797 Thu, 27 Aug 2009 23:00:00 +0100 2743797 http://www.medworm.com/index.php?rid=2724404&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F29 Conclusions: This large-scale analysis provides a framework for understanding the cooperation between E6 and E7 oncoproteins in HPV-driven carcinogenesis. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2724404 Sat, 22 Aug 2009 23:00:00 +0100 2724404 http://www.medworm.com/index.php?rid=2688328&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F28 In this study, the acute effects of IGF-1 on the proteome of murine C2C12 cells were examined. Cells were treated with IGF-1 for up to 24 hours, lysed, and fractionated into cytosolic, nuclear, and insoluble portions. Proteins from the cytosolic fraction were further separated using a new batch ion-exchange chromatography method to reduce sample complexity, followed by twodimensional (2D) electrophoresis, and identification of selected proteins by mass spectrometry.PDQuest software was utilized to identify and catalogue temporal changes in protein expression during IGF-1 stimulation. In response to IGF-1 stimulation, expression of 23 proteins increased at least three-fold and expression of 17 proteins decreased at least three-fold compared with control un-stimulated C2C12 cells. Changes in... Proteome Science journals http://www.medworm.com/rss/comments.php?id=2688328 Mon, 10 Aug 2009 23:00:00 +0100 2688328 http://www.medworm.com/index.php?rid=2683720&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F27 Conclusions: We present an accurate prediction method using a machine-learning approach based solely on protein sequence properties. The primary contribution of this paper is to propose new PNPRD features representing global and/or local differences in sequences, based on positively and/or negatively charged residues, to assist in predicting protein function. In addition, we identified a compact and useful feature subset for predicting the function of various proteins. Our results indicate that sequence-based classifiers can provide good results among a broad range of proteins, that the proposed features are useful in predicting several functions, and that the combination of our and traditional features may support the creation of a discriminative feature set for specific protein functions... Proteome Science journals http://www.medworm.com/rss/comments.php?id=2683720 Sat, 08 Aug 2009 23:00:00 +0100 2683720 http://www.medworm.com/index.php?rid=2653429&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F26 Conclusion: We describe novel approaches of subcellular proteomic profiling and assessment of acute changes on a minute-based time scale. These data expand the current knowledge of acute, non-transcriptional activities of glucocorticoids, including changes in protein subcellular localization, altered translation of quiescent RNA pools, and PKC-mediated cytoskeleton remodeling. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2653429 Wed, 29 Jul 2009 23:00:00 +0100 2653429 http://www.medworm.com/index.php?rid=2610643&cid=s_34089_79_f&fid=34089&url=http%3A%2F%2Fwww.proteomesci.com%2Fcontent%2F7%2F1%2F25 Conclusions: The results from this study revealed changes among both neuronal protein processing and endogenous proteolysis modulators in EAE animals. This information may provide a rationale for protease inhibitor-based therapeutic interventions for demyelinating diseases and multiple sclerosis. (Source: Proteome Science) Proteome Science journals http://www.medworm.com/rss/comments.php?id=2610643 Wed, 15 Jul 2009 23:00:00 +0100 2610643