MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Reproductive Biology and Endocrinology' source. Wed, 08 Feb 2012 15:09:03 +0100 http://www.medworm.com/index.php?rid=5667679&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F9 Variability in the subfertile patient population excludes the possibility of a single approach to controlled ovarian stimulation (COS) covering all the requirements of a patient. Modern technology has led to the development of new drugs, treatment options and quantitative methods that can identify single patient characteristics. These could potentially be used to match patients with the right treatment options to optimise efficacy, safety and tolerability during COS. Currently, age and follicle-stimulating hormone (FSH) level remain the most commonly used single patient characteristics in clinical practice. These variables only provide a basic prognosis for success and indications for standard COS treatment based on gross patient categorisation. In contrast, the anti-Mullerian hormone leve... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5667679 Mon, 06 Feb 2012 05:00:00 +0100 5667679 http://www.medworm.com/index.php?rid=5667678&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F10 Conclusions: Irrespective of PCOS status, women with IGT had higher serum preptin levels compared with women with NGT. Preptin levels are related with glucose tolerance status, but not with PCOS status. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5667678 Mon, 06 Feb 2012 05:00:00 +0100 5667678 http://www.medworm.com/index.php?rid=5667677&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F11 Conclusions: HBOT of six weeks itself, changed ovarian morphology in favor of atresia both in PCO group and control group. This result of aggravated follicular atresia after HBOT on EV induced PCO may be due to long-term exposure in our protocol which with this state seems to be inapplicable in the improvement of PCO morphology. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5667677 Mon, 06 Feb 2012 05:00:00 +0100 5667677 http://www.medworm.com/index.php?rid=5648760&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F7 Ultrasound has been used in rats to produce a Sperm Count Index of zero. The non-invasive and reversible nature of this treatment makes it a promising candidate for a male contraceptive. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5648760 Mon, 30 Jan 2012 05:00:00 +0100 5648760 http://www.medworm.com/index.php?rid=5648759&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F8 Conclusions: In a similar dose to that observed in serum following oral intake for EC, LNG had no effect on the number of motile spermatozoa recovered from the human fallopian tubes in vitro, on their adhesion to the tubal epithelium, distribution or AR rate. The possible effect of LNG as EC on sperm function remains poorly understood. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5648759 Mon, 30 Jan 2012 05:00:00 +0100 5648759 http://www.medworm.com/index.php?rid=5638923&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F6 Conclusions: In conclusion, dHuEPO tg mice caused excessive erythrocytosis that led to abnormal blood composition, short lifespan, and abnormal splenomegaly. Further, we identified 2,672 genes associated with splenomegaly by microarray analysis. These results could be useful in the development of dHuEPO-producing tg animals. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5638923 Fri, 27 Jan 2012 05:00:00 +0100 5638923 http://www.medworm.com/index.php?rid=5629396&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F5 Conclusions: Andronate with HFD rat model showed ovarian and metabolic features of PCOS, significant increase in glucose Ra, GNG, and lipid profiles, as well as normal blood glucose levels. Therefore, aberrant IR, increased glucose Ra, GNG, and lipid metabolism may represent the early-stage of glucose and lipid kinetics disorder, thereby might be used as potential early-stage treatment targets for PCOS. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5629396 Wed, 25 Jan 2012 05:00:00 +0100 5629396 http://www.medworm.com/index.php?rid=5620658&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F4 The objective of this study was to determine whether gamma tocopherol's (gT) angiogenic activity in placental network is enhanced via promoting interleukins. Methods: Pregnant ewes (N=18) were supplemented, orally, with 500 mg of alpha tocopherol (aT; N=6) or 1000 mg of gT (N=7) or placebo (CON; N=5) once daily from 107 to 137 days post breeding. Uterine and placental tissue samples were obtained at the end of supplementation to evaluate relative mRNA expressions of IL-1b, IL-6, IL-8, Tumor Necrosis Factor (TNF) alpha, Vascular Endothelial Growth Factor (VEGF), kinase insert domain receptor (KDR; VGFR2; a type III receptor tyrosine kinase), and soluble fms-like tyrosine kniase-1 (sFlt1 or sVEGFR1) in uterus, caruncle and cotyledon. Results: Oral supplementation of gT increased IL-6, IL-8, ... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5620658 Mon, 23 Jan 2012 05:00:00 +0100 5620658 http://www.medworm.com/index.php?rid=5609579&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F3 Conclusions: Regulation of EPs and FP expression by estradiol appears to be mainly modulated via ERalpha for EP1, EP3 and FP, while EP2 and EP4 also are affected by the ERbeta selective ligand. Our immunohistochemical data shows a cell specific regulation of prostaglandin receptors under the influence of ovarian steroids, where EP2 is estrogen regulated in all uterine tissues examined. EP1 and EP3 are upregulated by the combination of estradiol and progesterone. Thus, our observations indicate that estradiol and progesterone regulate the mRNA and protein expression of EPs and FP in a receptor and tissue specific way. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5609579 Wed, 18 Jan 2012 05:00:00 +0100 5609579 http://www.medworm.com/index.php?rid=5598385&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F2 Conclusions: EPC proliferation is induced during the menstrual phase and proliferation can be affected by estrogen through ERalpha activation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5598385 Tue, 17 Jan 2012 05:00:00 +0100 5598385 http://www.medworm.com/index.php?rid=5582812&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F10%2F1%2F1 Background: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. Methods: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women. Results: There were significantly lower levels of HOXA11 transcripts (p=0.003, p=0.041) and protein (p=0.004, p=0.001) in women with endometriosis as compared to fertile women and infertile women w... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5582812 Tue, 10 Jan 2012 05:00:00 +0100 5582812 http://www.medworm.com/index.php?rid=5538447&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F161 Conclusions: Pre-treatment measurement of sLHCGR could be used to tailor individual fertility treatment programs and improve outcomes by avoiding ovarian hyperstimulation and poor embryo implantation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5538447 Fri, 23 Dec 2011 05:00:00 +0100 5538447 http://www.medworm.com/index.php?rid=5538448&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F160 Conclusions: Our study suggests that low maternal care alters corticosterone and 17beta-estradiol levels, disrupting the estrous cycle and folliculogenesis and differentially regulating the expression of ER-alpha and ER-beta in the ovaries of adult rats. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5538448 Thu, 22 Dec 2011 05:00:00 +0100 5538448 http://www.medworm.com/index.php?rid=5515789&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F159 Conclusion: In this study, follicle culture system that can reproduce theca cell layer formation in vitro was established. In our opinion, this system is suitable for the analysis of theca cell layer formation and contributes to our understanding of the mechanisms of folliculogenesis. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5515789 Sat, 17 Dec 2011 05:00:00 +0100 5515789 http://www.medworm.com/index.php?rid=5515790&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F158 Conclusion: This finding provides evidence that polyalanine repeat expansions in FOXE1 may be responsible for the genetic aetiology of POF in Chinese women. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5515790 Fri, 16 Dec 2011 05:00:00 +0100 5515790 http://www.medworm.com/index.php?rid=5505750&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F157 Conclusions: This study has defined the effective dose and timing of EDP treatment for inducing pseudopregnancy in cyclic pigs. Our results also indicated that EDP caused a lowering of inhibin concentrations during pseudopregnancy and small numbers of follicles from 20 to 28 days after EDP. In contrast, EDP-induced pseudopregnancy appears to have no adverse effect on follicular development and subsequent ovulation following PGF2alpha administration. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5505750 Wed, 14 Dec 2011 05:00:00 +0100 5505750 http://www.medworm.com/index.php?rid=5495205&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F156 Conclusions: Vitrification is superior to the slow freezing method in terms of the survival and developmental rates for the cryopreservation of human failed-matured oocytes. In addition, GV oocytes appeared to be more resistant than MI oocytes to the low temperature and cryoprotectant used during cryopreservation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5495205 Mon, 12 Dec 2011 05:00:00 +0100 5495205 http://www.medworm.com/index.php?rid=5487643&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F155 Conclusion: Both HDSC and THESC may represent suitable model systems for cAMP-dependent in vitro decidualization. Since cAMP decreases cell viability of HDSC after 6 days of incubation, this substance should be preferentially used in short-term experiments. Progesterone treatment of THESC might not be applicable since these cells lack progesterone response and PR protein. In contrast, stimulation of PR-expressing HDSC with E2P4 or cAMP/E2P4 may represent an appropriate protocol for human in vitro decidualization inducing and maintaining expression of critical marker genes in a time-dependent manner. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5487643 Wed, 07 Dec 2011 05:00:00 +0100 5487643 http://www.medworm.com/index.php?rid=5477046&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F154 Conclusions: It is difficult to isolate the effects directly from diabetes and those from IUGR. Although the exposure to hyperglycemic environment during prenatal life and lactation delayed the onset of puberty in male rats, the IUGR, in the studied model, did not influenced the structural organization of the male gonads of the offspring at any point during sexual development. However the decrease in sperm reserves in epididymal cauda and the acceleration in sperm transit time in this portion of epididymis may lead to an impairment of sperm quality and fertility potential in these animals. Additional studies are needed in attempt to investigate the fertility of animals with intrauterine growth restriction by maternal diabetes and possible multigenerational effects. (Source: Reproductive B... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5477046 Tue, 06 Dec 2011 05:00:00 +0100 5477046 http://www.medworm.com/index.php?rid=5469453&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F153 Conclusions: While serum AMH has found increasing application as a predictor of ovarian reserve for patients prior to IVF, its roles to estimate in vitro embryo morphology and potential to advance to blastocyst stage have not been extensively investigated. These data suggest that baseline serum AMH determinations can help forecast blastocyst developmental during IVF. Serum AMH measured before treatment may assist patients, clinicians and embryologists as scheduling of embryo transfer is outlined. Additional studies are needed to confirm these correlations and to better define the role of baseline serum AMH level in the prediction of blastocyst formation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5469453 Fri, 02 Dec 2011 05:00:00 +0100 5469453 http://www.medworm.com/index.php?rid=5458407&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F152 Conclusions: The association of higher AEA levels with early pregnancy failure and with beta-hCG and PAPP-A, but not with progesterone concentrations suggest that plasma AEA levels and pregnancy failure are linked via a mechanism that may involve trophoblastic beta-hCG, and PAPP-A, but not, progesterone production. Although the trophoblast, decidua and embryo contain receptors for AEA, the main AEA target in early pregnancy failure remains unknown. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5458407 Tue, 29 Nov 2011 05:00:00 +0100 5458407 http://www.medworm.com/index.php?rid=5449944&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F151 The objective of this study was to compare the effect of a CIDR-Select Synch and a 12-d CIDR protocol on the pregnancy rate in healthy, multiparous dairy cows in Swiss dairy farms. Methods: Cows (N=508) were randomly assigned to CIDR-Select Synch (N=262) or 12-d CIDR (N=246) protocols. Cows in the CIDR-Select Synch group received a CIDR and 2.5 ml of buserelin i.m. on d 0. On d 7, the CIDR insert was removed and 5 ml of dinoprost was administered i.m.. Cows in the 12-d CIDR group received the CIDR on d 0 and it was removed on d 12 (the routine CIDR protocol in Swiss dairies). On d 0 a milk sample for progesterone analysis was taken. Cows were inseminated upon observed estrus. Pregnancy was determined at or more than 35 days after artificial insemination. As a first step, the two groups wer... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5449944 Fri, 25 Nov 2011 05:00:00 +0100 5449944 http://www.medworm.com/index.php?rid=5441595&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F150 Background: In the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility of vitrifying in vitro matured oocytes (IVM) isolated at the time of ovarian tissue cryopreservation to improve the efficiency of fertility preservation programs. Methods: Oocyte-cumulus complexes were retrieved from freshly collected ovarian cortex by aspirating antral follicular fluid, and were matured in vitro for 24-48 h prior to vitrification. Oocytes were matured in an IVM commercial medium (Copper Surgical, USA) supplemented with 75 mIU/ml FSH and 75 mIU/ml LH and vitrified using a commer... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5441595 Wed, 23 Nov 2011 05:00:00 +0100 5441595 http://www.medworm.com/index.php?rid=5422718&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F149 Cortical granules are membrane bound organelles located in the cortex of unfertilized oocytes. Following fertilization, cortical granules undergo exocytosis to release their contents into the perivitelline space. This secretory process, which is calcium dependent and SNARE protein-mediated pathway, is known as the cortical reaction. After exocytosis, the released cortical granule proteins are responsible for blocking polyspermy by modifying the oocytes' extracellular matrices, such as the zona pellucida in mammals. Mammalian cortical granules range in size from 0.2 um to 0.6 um in diameter and different from most other regulatory secretory organelles in that they are not renewed once released. These granules are only synthesized in female germ cells and transform an egg upon sperm entry; t... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5422718 Thu, 17 Nov 2011 05:00:00 +0100 5422718 http://www.medworm.com/index.php?rid=5388056&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F147 Ovarian hyperstimulation syndrome (OHSS) still remains a life-threatening complication of in vitro fertilization treatment (IVF), keeping patients and especially those, who previously experienced OHSS, from attempting infertility treatment and childbearing. The recent implementation of four new modalities: the GnRH antagonist protocol, GnRH agonist (GnRHa) triggering of ovulation, blastocyst transfer and embryo/oocyte vitrification, renders feasible the elimination of OHSS in connection with ovarian hyperstimulation for IVF treatment. The proposed current algorithm is based on the number of follicles developed after ovarian stimulation, setting a cut-off level at the development of 18 or more follicles. Further, fulfilling this criterion, the algorithm is based on four decision-making poin... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388056 Thu, 03 Nov 2011 04:00:00 +0100 5388056 http://www.medworm.com/index.php?rid=5388055&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F148 Conclusions: A comprehensive analysis of CXCL12 and CXCR4 expression in fetal and maternal tissues during early pregnancy is reported with noteworthy differences occurring during implantation and placentation in sheep. We interpreted these data to mean that the CXCL12/CXCR4 pathway is activated during implantation and placentation in sheep and is likely playing a role in the communication between trophoblast cells and the maternal endometrium. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388055 Thu, 03 Nov 2011 04:00:00 +0100 5388055 http://www.medworm.com/index.php?rid=5388058&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F145 Background: Ficus platyphylla Delile (family- Moracea) commonly called gutta percha tree is a deciduous plant found in savannah areas. It grows widely in the Northern part of Nigeria, up to 60ft. high and is known as 'gamji' by the Hausas. The seeds, bark and leaves have been used traditionally in combination to promote fertility. Scientifically, the plant has been shown to have analgesic, anti-inflammatory and CNS effects. The present study was to validate the use of this plant to promote fertility in female Rattus norvegicus Wistar strain using various fertility parameters. Methods: Female Rattus norvegicus Wistar strain weighing between 150-180g were randomly selected and divided into two major groups. Each group was subdivided into 5 treatment groups of 100, 200, 400 mg/kg BW of aqueou... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388058 Wed, 02 Nov 2011 04:00:00 +0100 5388058 http://www.medworm.com/index.php?rid=5388057&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F146 In this report we summarize the recent evidence that vitamin D status influences female reproductive and pregnancy outcomes. Human and animal data suggest that low vitamin D status is associated with impaired fertility, endometriosis and polycystic ovary syndrome. Evidence from observational studies shows higher rates of preeclampsia, preterm birth, bacterial vaginosis and gestational diabetes in women with low vitamin D levels. However, confirmation of experimental observations establishing an association of vitamin D deficiency with adverse reproductive outcomes by high quality observational and large-scale randomized clinical trials is still lacking. The determination of optimal 25(OH)D3 levels in the reproductive period and the amount of vitamin D supplementation required to achieve th... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388057 Wed, 02 Nov 2011 04:00:00 +0100 5388057 http://www.medworm.com/index.php?rid=5388060&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F143 Conclusions: Despite some promising results, it seems too early to conclude that low O2 culture has an effect on IVF outcome. Additional randomised controlled trials are necessary before evidence-based recommendations can be provided. It should be emphasised that the present meta-analysis does not provide any evidence that low oxygen concentration is unnecessary. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388060 Tue, 01 Nov 2011 04:00:00 +0100 5388060 http://www.medworm.com/index.php?rid=5388059&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F144 Conclusions: These data from a large representative and non selected population of women confirm the concept that IH and PCOS are the two most common gynecological endocrinopathies among reproductive aged women. The estimated prevalence of these conditions is highly influenced by their screening methods at the community level. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5388059 Tue, 01 Nov 2011 04:00:00 +0100 5388059 http://www.medworm.com/index.php?rid=5348396&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F141 Conclusions: The two-step enzyme digestion (by type I collagenase and trypsin) process is an economical, simple and reproducible technique for isolating human spermatogonial stem cells. With little contamination and less cell damage, this method facilitates isolated human spermatogonial stem cells to form a stable cell colony on the supporting cell layer. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5348396 Mon, 24 Oct 2011 04:00:00 +0100 5348396 http://www.medworm.com/index.php?rid=5348395&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F142 Conclusions: Monofollicular growth rate was comparable with optimal rates reported elsewhere and the pregnancy rate exceeded that in other studies of OI using gonadotropins. A starting dose of 37.5 IU/day follitropin alfa is an effective option in selected cases to prevent ovarian hyper-response without loss of efficacy. The analysis could not identify a single selection criterion for individuals who would benefit from this treatment approach; this merits further investigation in prospective studies. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5348395 Mon, 24 Oct 2011 04:00:00 +0100 5348395 http://www.medworm.com/index.php?rid=5336727&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F140 Conclusions: Our proof-of-concept study demonstrates that a site-specific hysteroscopic endometrium injury performed during the ongoing in vitro fertilization (IVF) cycle, instead of injuries received during prior cycles, significantly improves clinical outcomes in patients with repeated implantation failure. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5336727 Fri, 21 Oct 2011 04:00:00 +0100 5336727 http://www.medworm.com/index.php?rid=5336728&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F139 Conclusions: Our study demonstrated that AKR1C1 mRNA and protein are coordinately expressed in the luteal cell of ovary throughout the estrous cycle and in the uterus on day 30 of pregnancy. Thus, the porcine AKR1C1 gene might control important mechanisms during the estrous cycle. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5336728 Thu, 20 Oct 2011 04:00:00 +0100 5336728 http://www.medworm.com/index.php?rid=5315183&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F138 Conclusions: Together, these results provide in vivo evidence of important TR4 roles in penile physiology, as well as in male sexual behavior. In conjunction with previous finding, TR4 represents a key factor that controls male fertility via regulating behavior and internal physiological events. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5315183 Thu, 13 Oct 2011 04:00:00 +0100 5315183 http://www.medworm.com/index.php?rid=5297449&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F137 Conclusions: Collectively, results of the present study indicate that A2M is novel estrogen-stimulated gene expressed in LE and GE of the chicken oviduct and may be used for monitoring effects of therapies for ovarian cancer in laying hens. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5297449 Fri, 07 Oct 2011 04:00:00 +0100 5297449 http://www.medworm.com/index.php?rid=5297450&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F136 Conclusions: In this study, we provide examples of in vitro and in vivo tests that may be used to evaluate embryotoxicity for dental materials. Our results show that leached components from our composite-material induced embryotoxicity in vitro, however, no toxicity was observed when subcutaneously implanted in vivo. This highlights the necessity of integrated in vitro and in vivo tests for valuable predictive estimation of embryotoxicity for complex materials. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5297450 Thu, 06 Oct 2011 04:00:00 +0100 5297450 http://www.medworm.com/index.php?rid=5286277&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F134 Conclusions: Apparently, the mycotoxin zearalenone has 17beta-estradiol-like estrogenic activity that enables it to induce hyperactivated motility of equine sperm cells, whereas the zearalenol derivatives induce premature completion of the acrosome reaction and thereby adversely affect stallion sperm physiology. The alpha form of zearalenol still possessed the estrogenic ability to induce hyperactivated motility, whereas its beta stereo-isomere had lost this property. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5286277 Wed, 05 Oct 2011 04:00:00 +0100 5286277 http://www.medworm.com/index.php?rid=5286276&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F135 Conclusions: These findings suggest that GPER could mediate estrogen signaling in both normal and transformed somatic cells of human testis, but they reveal a differential expression of the novel estrogen receptor in non neoplastic and neoplastic germ cells. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5286276 Wed, 05 Oct 2011 04:00:00 +0100 5286276 http://www.medworm.com/index.php?rid=5286280&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F131 Conclusion: This is the first study on the -1031(T/C) polymorphism of TNF-alpha gene in PCOS. We concluded that the -1031(T/C) polymorphism of TNF-alpha gene is associated with PCOS in a Korean population. Therefore, it is possible that it may be considered as a clinical biomarker to diagnose for PCOS, and is helpful in understanding the etiology for the pathogenesis of PCOS. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5286280 Tue, 04 Oct 2011 04:00:00 +0100 5286280 http://www.medworm.com/index.php?rid=5286279&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F132 Conclusions: The spatial and temporal expression profiles of CatSper genes were reported herein for the first time in pigs. CatSper1, CatSper2 and CatSper4 were primarily expressed in testes, while CatSper3 transcript was prevalent in a variety of organs. CatSper3 and CatSper4 mRNAs were present in mature sperm cells. Substantial upregulation of CatSper genes was initiated at Day 60 and maintained this marked production until sexual maturity. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5286279 Tue, 04 Oct 2011 04:00:00 +0100 5286279 http://www.medworm.com/index.php?rid=5286278&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F133 Conclusions: The identification of the three intracellular NOS isoforms suggests that pig spermatozoa could produce NO, while the augmented nitric oxide levels in leptin- treated male gametes indicates the capacity of the hormone to induce nitric oxide production. Furthermore, the inhibitory effect of L-NAME and of Ab-ObR on the promotion of acrosome reaction triggered by leptin suggests a possible involvement of NO in the hormone action.. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5286278 Tue, 04 Oct 2011 04:00:00 +0100 5286278 http://www.medworm.com/index.php?rid=5274539&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F130 Conclusions: The annual rhythm driven by melatonin secretion is only responsible for the timing of the breeding season. The occurrence and length of winter ovarian inactivity is defined by metabolic hormones. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5274539 Thu, 29 Sep 2011 04:00:00 +0100 5274539 http://www.medworm.com/index.php?rid=5261427&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F129 Conclusions: Predisposition to produce anti-sperm antibodies seems to be only weakly associated with HLA class II genes, although this question requires further study on much larger population sample. It is plausible that familial and sporadic cryptorchidism may present distinct genetic background. The same may, to lower extent, apply to bilateral and unilateral cryptorchidism. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5261427 Wed, 28 Sep 2011 04:00:00 +0100 5261427 http://www.medworm.com/index.php?rid=5261428&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F128 Conclusions: We have characterized a gene cluster consisting of at least 14 expressed Pate gene members, including Acrv1, Sslp1 and a previously uncharacterized gene which we named Pate-X. The genes code for putatively secreted, cysteine-rich proteins with a TFP/Ly-6/uPAR domain. Members of the Pate gene cluster characterized are predominantly expressed in the murine epididymis, not in the testis or prostate, and are regulated by testicular factors. Similar proteins are present in venoms of several reptiles, and they are thought to mediate their effects by regulating certain ion channels, and are thus expected to have a clinical relevance in sperm maturation and epididymal infections. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5261428 Mon, 26 Sep 2011 04:00:00 +0100 5261428 http://www.medworm.com/index.php?rid=5226794&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F127 Conclusions: Mono- or multicentre quality control on embryo scoring by morphological assessment can easily be performed through the design of a simple website. In the future the website design can be adapted to generate statistical feedback upon scoring and can even include a training module. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5226794 Thu, 15 Sep 2011 04:00:00 +0100 5226794 http://www.medworm.com/index.php?rid=5226795&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F126 Conclusions: Collectively, these data help to determine NP's actions on mouse TM4 Sertoli cells and increase our understanding of the molecular mechanisms underlying the adverse effects of xenoestrogens on the reproductive system. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5226795 Wed, 14 Sep 2011 04:00:00 +0100 5226795 http://www.medworm.com/index.php?rid=5226796&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F125 In this study we test the competing hypothesis of the relationship between food insecurity and age at menarche among adolescent girls in the Southwest Ethiopia. Methods: We report on 900 girls who were investigated in the first two rounds of the five year longitudinal survey. The semi-parametric frailty model was fitted to determine the effect of adolescent food insecurity on time to menarche after adjusting for socio-demographic and economic variables. Results: Food insecure girls have menarche one year later than their food secure peer (median age of 15 years vs 14 years). The hazard of menarche showed a significant decline (P=0.019) as severity of food insecurity level increased, the hazard ratio (HR) for mild food insecurity and moderate/severe food insecurity were 0.936 and 0.496, res... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5226796 Tue, 13 Sep 2011 04:00:00 +0100 5226796 http://www.medworm.com/index.php?rid=5209603&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F124 Conclusions: Simultaneous measurement of leptin with several inflammatory molecules and angiogenic factors in this study enabled us to investigate their relationship, which can help to understand the role of circulating leptin in normal pregnancy and preeclampsia. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5209603 Fri, 09 Sep 2011 04:00:00 +0100 5209603 http://www.medworm.com/index.php?rid=5170739&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F123 Conclusions: The IMSI procedure improved embryo development and the laboratory and clinical outcomes of sperm microinjection in the same infertile couples with male infertility and poor embryo development over the previous ICSI attempts. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5170739 Sun, 28 Aug 2011 23:00:00 +0100 5170739 http://www.medworm.com/index.php?rid=5162736&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F122 Conclusion: Neuromuscular Electrical stimulation therapy may be effective for the patients with a thin endometrium. Further studies are needed to investigate its effectiveness. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5162736 Wed, 24 Aug 2011 23:00:00 +0100 5162736 http://www.medworm.com/index.php?rid=5162737&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F121 Background: The in vitro culture of presumed zygotes derived from single cow ovum pick-up (OPU) is important for the production of quality blastocysts maintaining pedigree. The aim of the present study was to evaluate the agar chip-embedded helper embryo coculture system for single cow OPU-derived zygotes by assessing embryo quality. Methods: Cumulus oocyte complexes (COCs) were collected from Hanwoo cows with high genetic merit twice a week using the ultra-sound guided OPU technique and from slaughterhouse ovaries. The Hanwoo cow COCs and slaughterhouse ovaries were matured in vitro, fertilized in vitro with thawed Hanwoo sperm and cultured for 24 h. The presumed zygotes were subsequently placed in three different culture systems: (1) control OPU (controlOPU) with single cow OPU-derived p... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5162737 Tue, 23 Aug 2011 23:00:00 +0100 5162737 http://www.medworm.com/index.php?rid=5162740&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F118 Conclusions: Here, we demonstrate that mDCs and pDCs secrete alpha-defensins 1-3 and present a novel effect of E2 on the secretion of alpha-defensins 1-3 by dendritic cells. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5162740 Mon, 22 Aug 2011 23:00:00 +0100 5162740 http://www.medworm.com/index.php?rid=5162739&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F119 This report describes an unusual case in which persistent ovarian cysts are associated with primary hypothyroidism in a young woman. The data were collected by history-taking, physical examination, laboratory tests, ultrasound, magnetic resonance imaging and a histo-pathological study. In addition, the exons of the gene encoding the human follicle-stimulating hormone receptor were sequenced.DiscussionThe patient had markedly elevated levels of thyroid-stimulating hormone and follicle-stimulating hormone and an enlarged pituitary gland. After treatment with thyroid hormone replacement, regression of the enlarged pituitary and the ovarian cysts was observed. The possible mechanisms of the pathophysiology are discussed below.SummaryIt is necessary to consider hypothyroidism and other endocrin... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5162739 Mon, 22 Aug 2011 23:00:00 +0100 5162739 http://www.medworm.com/index.php?rid=5162738&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F120 Conclusions: The present findings support the use of high-magnification microscopy both for selecting spermatozoa and as a routine method for analysing semen before performing IUI. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5162738 Mon, 22 Aug 2011 23:00:00 +0100 5162738 http://www.medworm.com/index.php?rid=5144550&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F117 Conclusions: Our study suggests that p97/VCP and Jab1/CSN5 could be an important part of the UPS in the developing rat testis and epididymis and that both proteins may be involved in the regulation of spermatogenesis and epididymal epithelial functions. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5144550 Thu, 18 Aug 2011 23:00:00 +0100 5144550 http://www.medworm.com/index.php?rid=5144551&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F116 DiscussionSelective androgens appear capable of improving early stages of folliculogenesis. They, therefore, may represent forerunners of a completely new class of ovulation-inducing medications, which, in contrast to gonadotropins, affect follicle maturation at much earlier stages. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5144551 Tue, 16 Aug 2011 23:00:00 +0100 5144551 http://www.medworm.com/index.php?rid=5132634&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F115 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5132634 Sun, 14 Aug 2011 23:00:00 +0100 5132634 http://www.medworm.com/index.php?rid=5120185&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F114 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5120185 Thu, 11 Aug 2011 23:00:00 +0100 5120185 http://www.medworm.com/index.php?rid=5110247&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F111 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5110247 Mon, 08 Aug 2011 23:00:00 +0100 5110247 http://www.medworm.com/index.php?rid=5110246&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F112 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5110246 Mon, 08 Aug 2011 23:00:00 +0100 5110246 http://www.medworm.com/index.php?rid=5110245&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F113 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5110245 Mon, 08 Aug 2011 23:00:00 +0100 5110245 http://www.medworm.com/index.php?rid=5088978&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F109 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5088978 Tue, 02 Aug 2011 23:00:00 +0100 5088978 http://www.medworm.com/index.php?rid=5088977&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F110 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5088977 Tue, 02 Aug 2011 23:00:00 +0100 5088977 http://www.medworm.com/index.php?rid=5088981&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F106 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5088981 Mon, 01 Aug 2011 23:00:00 +0100 5088981 http://www.medworm.com/index.php?rid=5088980&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F107 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5088980 Mon, 01 Aug 2011 23:00:00 +0100 5088980 http://www.medworm.com/index.php?rid=5088979&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F108 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5088979 Mon, 01 Aug 2011 23:00:00 +0100 5088979 http://www.medworm.com/index.php?rid=5080484&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F104 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5080484 Sun, 31 Jul 2011 23:00:00 +0100 5080484 http://www.medworm.com/index.php?rid=5080483&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F105 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5080483 Sun, 31 Jul 2011 23:00:00 +0100 5080483 http://www.medworm.com/index.php?rid=5080486&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F102 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5080486 Thu, 28 Jul 2011 23:00:00 +0100 5080486 http://www.medworm.com/index.php?rid=5080485&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F103 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5080485 Thu, 28 Jul 2011 23:00:00 +0100 5080485 http://www.medworm.com/index.php?rid=5080487&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F101 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5080487 Wed, 27 Jul 2011 23:00:00 +0100 5080487 http://www.medworm.com/index.php?rid=5070464&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F100 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5070464 Tue, 26 Jul 2011 23:00:00 +0100 5070464 http://www.medworm.com/index.php?rid=5053374&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F99 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5053374 Thu, 21 Jul 2011 23:00:00 +0100 5053374 http://www.medworm.com/index.php?rid=5053375&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F98 ${item.shortDescription} (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=5053375 Wed, 20 Jul 2011 23:00:00 +0100 5053375 http://www.medworm.com/index.php?rid=4999685&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F97 This study adds bradykinin - acting on the B2R - to the stimuli of trophoblast migration and invasion, an effect that should be integrated to other modifications of the kallikrein-kinin system in normal and pathological pregnancies. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4999685 Mon, 04 Jul 2011 23:00:00 +0100 4999685 http://www.medworm.com/index.php?rid=4986732&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F95 Conclusions: Fractalkine and CX3CR1 were found to express in human ovary and luteinising granulosa cells. Fractalkine can increase the biosynthesis of progesterone in a dose-dependent manner by enhancing transcript levels of key steroidogenic enzymes. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4986732 Wed, 29 Jun 2011 23:00:00 +0100 4986732 http://www.medworm.com/index.php?rid=4986731&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F96 Fertilin alpha (ADAM-1) and beta (ADAM-2) are integral membrane proteins of the ADAM family that form a fertilin complex involved in key steps of the sperm-oocyte membrane interaction. In the present work, we analyzed the presence of ADAM-1 and ADAM-2 mRNAs, the spermatozoa proteins' processing and their sub-cellular localization in epididymal samples from adult boars. ADAM-1 and ADAM-2 mRNAs were highly produced in the testis, but also in the vas efferens and the epididymis. On immunoblots of sperm extracts, ADAM-1 subunit appeared as a main reactive band of ~50-55 kDa corresponding to occurrence of different isoforms throughout the epididymal duct, especially in the corpus region where isoforms ranged from acidic to basic pI. In contrast, ADAM-2 was detected as several bands of ~90 kDa, ... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4986731 Wed, 29 Jun 2011 23:00:00 +0100 4986731 http://www.medworm.com/index.php?rid=4961833&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F94 Background: Recent studies have supported the concept of "fetal programming" which suggests that during the intrauterine development the fetus may be programmed to develop diseases in adulthood. The possible effects of in utero protein restriction on sexual development of rat male offspring were evaluated in the present study. Methods: Pregnant Wistar rats were divided into two experimental groups: one group treated with standard chow (SC, n=8, 17% protein) and the other group treated with hypoproteic chow (HC, n=10, 6% protein) throughout gestation. After gestation the two experimental groups received standard chow. To evaluate the possible late reproductive effects of in utero protein restriction, the male offspring of both groups were assessed at different phases of sexual development: ... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4961833 Thu, 23 Jun 2011 23:00:00 +0100 4961833 http://www.medworm.com/index.php?rid=4986733&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F82 Conclusions: A variety of COS protocols have been adopted, with mixed success, but no single approach is appropriate for all patients within a given population. We suggest that treatment protocols should be adapted for individual patients through iCOS; this approach promises to be one of the first steps towards implementing personalised medicine in reproductive science. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4986733 Mon, 20 Jun 2011 23:00:00 +0100 4986733 http://www.medworm.com/index.php?rid=4961834&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F83 The objective of this study was to compare letrozole-stimulated cycles to natural cycles in 208 patients undergoing intrauterine insemination (IUI) between July of 2004 and January of 2007. Group I (n=47) received cycle monitoring only (natural group), Group II (n=125) received letrozole 2.5mg/day on cycle days three to seven, and Group III (n=36) received letrozole 5mg/day on cycle days three to seven. There were no differences between the groups in endometrial thickness or P4 on the day of hCG. Estradiol levels had higher variation in the second half of the follicular phase in both letrozole-treated groups compared to the control group. Estradiol per preovulatory follicle was similar in both letrozole cycles to that observed in the natural cycles. LH was lower on the day of hCG administr... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4961834 Mon, 20 Jun 2011 23:00:00 +0100 4961834 http://www.medworm.com/index.php?rid=4954931&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F84 Conclusion: The extended letrozole regimen had a superior efficacy as compared with clomiphene citrate in patients of unexplained infertility undergoing superovulation and IUI.ClinicalTrials.gov, NCT01232075 (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954931 Mon, 20 Jun 2011 23:00:00 +0100 4954931 http://www.medworm.com/index.php?rid=4954930&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F85 Aromatase inhibitors have been introduced as a new treatment modality that could challenge clomiphene citrate as an ovulation induction regiment in patients with PCOS. Although several randomized trials have been conducted regarding their use as ovulation induction agents, only few trials are available regarding their efficacy in IVF stimulated cycles. Current available evidence support that letrozole may have a promising role in stimulated IVF cycles, either when administered during the follicular phase for ovarian stimulation. Especially for women with poor ovarian response, letrozole appears to have the potential to increase clinical pregnancy rates when combined with gonadotropins, whereas at the same time reduces the total gonadotropin dose required for ovarian stimulation. However, g... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954930 Mon, 20 Jun 2011 23:00:00 +0100 4954930 http://www.medworm.com/index.php?rid=4954929&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F86 Clomiphene citrate has been traditionally used as the drug of choice in treating women with anovulatory infertility. In the last decade letrozole, an aromatase inhibitor has emerged as alternative ovulation induction agent. Literature confirms that letrozole has a definitive role in anovulatory women who have not responded to the clomiphene therapy. However its role as an alternative to clomiphene as first line therapy continues to be debated. Although it is probable that the overall benefits of letrozole surpass clomiphene citrate, currently available data does not confirm this view. There is need for large well-designed trials. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954929 Mon, 20 Jun 2011 23:00:00 +0100 4954929 http://www.medworm.com/index.php?rid=4954928&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F87 This article will review the rationale behind the use of aromatase inhibitors in treating endometriosis and summarize those studies which have evaluated the use of aromatase inhibitors in the treatment of endometriosis and its associated symptoms. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954928 Mon, 20 Jun 2011 23:00:00 +0100 4954928 http://www.medworm.com/index.php?rid=4954927&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F88 Conclusions: Aromatase inhibitors reduce the intensity of endometriosis-related pain symptoms. Combining letrozole with oral norethisterone acetate was associated with a lower incidence of adverse effects and a lower discontinuation rate than combining letrozole with triptorelin. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954927 Mon, 20 Jun 2011 23:00:00 +0100 4954927 http://www.medworm.com/index.php?rid=4954926&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F89 In conclusion, AIs effectively reduce the severity of endometriosis-related pain symptoms. Since endometriosis is a chronic disease, future investigations should clarify whether the long-term administration of AIs is superior to currently available endocrine therapies in terms of improvement of pain, adverse effects and patient satisfaction. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954926 Mon, 20 Jun 2011 23:00:00 +0100 4954926 http://www.medworm.com/index.php?rid=4954925&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F90 Postmenopausal endometriosis is a rare clinical condition. The diagnosis and treatment of an endometriotic lesion in postmenopausal women is complicated. First line treatment choice should be surgical, given that there is a potential risk of malignancy. Medical treatment may be considered as second line or as an alternate first line treatment whenever surgery is contradicted and aims to alter the hormonal pathway leading to endometriosis progress. Different hormonal regimens have been administered to these patients, with conflicting however results. Aromatase inhibitors (AIs) represent one of the most recently used drugs for postmenopausal endometriosis. Clinical data for the use of (AIs) in postmenopausal patients is scarce. Up to date only 5 case reports are available regarding the use o... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954925 Mon, 20 Jun 2011 23:00:00 +0100 4954925 http://www.medworm.com/index.php?rid=4954924&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F91 Aromatase, the key enzyme in estrogen biosynthesis, converts androstenedione to estrone and testosterone to estradiol. The enzyme is expressed in various tissues such as ovary, placenta, bone, brain, skin, and adipose tissue. Aromatase enzyme is encoded by a single gene CYP 19A1 and its expression is controlled by tissue-specific promoters. Aromatase mRNA is primarily transcribed from promoter I.4 in normal breast tissue and physiological levels of aromatase are found in breast adipose stromal fibroblasts. Under the conditions of breast cancer, as a result of the activation of a distinct set of aromatase promoters (I.3, II, and I.7) aromatase expression is enhanced leading to local overproduction of estrogen that promotes breast cancer. Aromatase is considered as a potential target for end... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954924 Mon, 20 Jun 2011 23:00:00 +0100 4954924 http://www.medworm.com/index.php?rid=4954923&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F92 Conclusions: This study demonstrates that a pharmacophore search using a model based on aromatase inhibition and the enzyme's structural features can be used to screen for new candidates for antiepileptic therapy. In fact, potent aromatase inhibitors and current antiepileptic compounds display significant - over 70% - chemical and structural similarity, and the similarity analyses performed propose a number of antiepileptic compounds with high potential for aromatase inhibition. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954923 Mon, 20 Jun 2011 23:00:00 +0100 4954923 http://www.medworm.com/index.php?rid=4954922&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F93 Aromatase inhibitors effectively delay epiphysial maturation in boys and improve testosterone levels in adult men Therefore, aromatase inhibitors may be used to increase adult height in boys with gonadotropin-independent precocious puberty, idiopathic short stature and constitutional delay of puberty. Long-term efficacy and safety of the use of aromatase inhibitors has not yet been established in males, however, and their routine use is therefore not yet recommended. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4954922 Mon, 20 Jun 2011 23:00:00 +0100 4954922 http://www.medworm.com/index.php?rid=4938310&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F81 In conclusion, ovarian endometriosis does not reduce IVF outcome compared with tubal factor. Furthermore, laparoscopic removal of endometriomas does not improve IVF results, but may cause a decrease of ovarian responsiveness to gonadotropins. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4938310 Thu, 16 Jun 2011 23:00:00 +0100 4938310 http://www.medworm.com/index.php?rid=4914386&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F80 Conclusions: Both ovarian endometrioma and cystectomy are associated with a significant reduction on ovarian reserve. Bilateral endometrioma exerts a more profound negative impact on ovarian reserve than unilateral endometrioma, regardless of either conservative or surgical intervention. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4914386 Wed, 08 Jun 2011 23:00:00 +0100 4914386 http://www.medworm.com/index.php?rid=4914389&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F77 The objective of this study was to undertake a systematic review to assess levels of sFlt-1 in early pregnancy and association with adverse pregnancy outcomes. PubMed and Medline databases and reference lists were searched up to July 2010. Inclusion criteria were pregnant women, blood sample taken during first trimester and assessment/reporting of sFlt-1 concentrations and subsequent pregnancy complications. Twelve relevant studies were identified of 71 to 668 women. No pooling of results was undertaken due to variation in sFlt-1 concentrations (range, 166-6,349pg/ml amongst controls), samples used (serum, plasma), different summary statistics (mean, median, odds ratio) and outcome definitions applied. Levels of sFlt-1 were generally higher among women who developed preeclampsia (11 studie... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4914389 Tue, 07 Jun 2011 23:00:00 +0100 4914389 http://www.medworm.com/index.php?rid=4914388&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F78 The objective of the present study is to assess viability tests and to evaluate follicle ovarian tissue quality after freezing-thawing procedures. Methods: Ewe's ovaries were harvested at the slaughterhouse, after dissection each ovarian specimen was divided into two groups: fresh tissue (control group) and frozen tissue.In the first part of the study, the follicles viability was assessed by trypan blue staining, calcein AM/ethidium homodimer-1 staining (LIVE/DEAD viability/cytotoxicity kit, Molecular Probes)and morphology in the two groups. In the second part of the study the quality of the whole ovarian tissue was evaluated by the quantification of the release of lactate dehydrogenase measurement (Cytotoxicity Detection kit ROCHE), DNA fragmentation by terminal deoxynucleotidyl transfera... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4914388 Tue, 07 Jun 2011 23:00:00 +0100 4914388 http://www.medworm.com/index.php?rid=4914387&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F79 Conclusion: PMNs migrate into the early CL partially due to its major chemoattractant IL-8 produced at high levels in the CL, and PMNs is a potential regulator of angiogenesis together with IL-8 in developing CL in the cow. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4914387 Tue, 07 Jun 2011 23:00:00 +0100 4914387 http://www.medworm.com/index.php?rid=4904527&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F76 Conclusion: No significant evidence of association or linkage was found in the four tested markers, indicating that our family samples did not support susceptibility of the INSR gene to PCOS. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4904527 Sun, 05 Jun 2011 23:00:00 +0100 4904527 http://www.medworm.com/index.php?rid=4877871&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F73 Conclusions: This is the first study to use a proteomics approach to identify hEEC plasma membrane proteins that may be useful as infertility markers or pharmacological targets for fertility regulation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4877871 Sun, 29 May 2011 23:00:00 +0100 4877871 http://www.medworm.com/index.php?rid=4877870&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F74 Background: The purpose of the study was to determine if the effect of llama OIF on LH secretion is mediated by stimulation of the hypothalamus or pituitary gland. Methods: Using a 2-by-2 factorial design to examine the effects of OIF vs GnRH with or without a GnRH antagonist, llamas with a growing ovarian follicle greater than or equal to 8 mm were assigned randomly to four groups (n=7 per group) and a) pre-treated with 1.5 mg of GnRH antagonist (cetrorelix acetate) followed by 1 mg of purified llama OIF, b) pre-treated with 1.5 mg of cetrorelix followed by 50 micrograms of GnRH, c) pre-treated with a placebo (2 ml of saline) followed by 1 mg of purified llama OIF or d) pre-treated with a placebo (2 ml of saline) followed by 50 micrograms of GnRH. Pre-treatment with cetrorelix or saline w... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4877870 Sun, 29 May 2011 23:00:00 +0100 4877870 http://www.medworm.com/index.php?rid=4877869&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F75 Conclusion: Although we cannot provide any evidence that it is a possible disease-causing gene, this study is the first attempt to investigate the possible role of WNT4 in Han Chinese women with POF. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4877869 Sun, 29 May 2011 23:00:00 +0100 4877869 http://www.medworm.com/index.php?rid=4873639&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F71 To elucidate the molecular mechanisms underling hydration during oocyte maturation, we characterized the structure of Japanese eel (Anguilla japonica) novel-water selective aquaporin 1 (AQP1b) that thought to be involved in oocyte hydration. The aqp1b cDNA encodes a 263 amino acid protein that includes the six potential transmembrane domains and two Asn-Pro-Ala motifs. Reverse transcription-polymerase chain reaction showed transcription of Japanese eel aqp1b in ovary and testis but not in the other tissues. In situ hybridization studies with the eel aqp1b cRNA probe revealed intense eel aqp1b signal in the oocytes at the perinucleolus stage and the signals became faint during the process of oocyte development. Light microscopic immunocytochemical analysis of ovary revealed that the Japanes... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4873639 Thu, 26 May 2011 23:00:00 +0100 4873639 http://www.medworm.com/index.php?rid=4873638&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F72 Conclusions: Our results demonstrate a characteristic expression of SERPIN superfamily member genes in bovine healthy and atretic follicles. The cell-type-and stage-specific expression of SERPINs may be associated with bovine follicular growth and atresia. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4873638 Thu, 26 May 2011 23:00:00 +0100 4873638 http://www.medworm.com/index.php?rid=4864903&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F70 Conclusion: SD rate from this study is high but similar to that reported among self-reported diabetic patients in Kumasi, Ghana and vary according to the condition and age. The determinants of SD from this study are income level, exercise, obesity, higher perception of "desirable" and "too short" IELT. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4864903 Tue, 24 May 2011 23:00:00 +0100 4864903 http://www.medworm.com/index.php?rid=4854685&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F69 Conclusions: Mating affects the kinetic of E2-induced expression of s100g although it not changed the cellular localization of s100g in the oviduct after E2. On the other hand, s100g is a functional component of E2 genomic effect that accelerates egg transport. These findings show a physiological involvement of s100g in the rat oviduct. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4854685 Sun, 22 May 2011 23:00:00 +0100 4854685 http://www.medworm.com/index.php?rid=4843297&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F68 Conclusion: Spermiation was most effectively induced in male P. guentheri by administering a single injection of LHRHa without priming. In contrast, female P. guentheri failed to ovulate without priming. A single priming injection induced the release of oocytes of high viability compared to oocytes obtained from females in the two priming treatment which underwent a process of over-ripening. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4843297 Thu, 19 May 2011 23:00:00 +0100 4843297 http://www.medworm.com/index.php?rid=4832283&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F67 DiscussionImprovement of oocyte/embryo quality with DHEA supplementation potentially suggests a new concept of ovarian aging, where ovarian environments, but notoocytes themselves, age. DHEA may, thus, represent a first agent beneficially affecting aging ovarian environments. Others can be expected to follow. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4832283 Mon, 16 May 2011 23:00:00 +0100 4832283 http://www.medworm.com/index.php?rid=4832285&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F65 Conclusions: Overall, the DOX results compared well with the conventional methods of checking proliferation of cells. The fascinating feature of the sensor array is the ability to provide continuous proliferation experiments with no additional reagents including 96 simultaneous electrochemical experiments. The use of the DOX-96 could reduce a typical bioassay time by 20-fold. Thus the DOX-96 can be used as both a research tool and for practical cell culture monitoring. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4832285 Sun, 15 May 2011 23:00:00 +0100 4832285 http://www.medworm.com/index.php?rid=4832284&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F66 Cystic ovarian disease (COD) is an important cause of abnormal estrous behavior and infertility in dairy cows. COD is mainly observed in high-yielding dairy cows during the first months post-partum, a period of high stress. We have previously reported that, in lower mammals, stress induces a cystic condition similar to the polycystic ovary syndrome in humans and that stress is a definitive component in the human pathology. To know if COD in cows is also associated with high sympathetic activity, we studied isolated small antral (5mm), preovulatory (10mm) and cystic follicles (25mm). Cystic follicles which present an area 600 fold greater compared with preovulatory follicles has only 10 times less concentration of NE as compared with small antral and preovulatory follicles but they had 10 t... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4832284 Sun, 15 May 2011 23:00:00 +0100 4832284 http://www.medworm.com/index.php?rid=4832287&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F64 Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. Secretion was minimal up to 72h but by 96h 24-37% of the LHCGR had been released into the culture medium. The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. Secretion was independent of recombinant size and was mediated via structurally defined membrane vesicles (50-150nm). Similarly culture... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4832287 Sat, 14 May 2011 23:00:00 +0100 4832287 http://www.medworm.com/index.php?rid=4832286&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F63 Conclusions: PIF is an early embryo viability marker, that has a direct supportive role on embryo development in culture. PIF-ELISA use to assess IVF embryo quality prior to transfer is warranted. Overall, our data supports PIF's endogenous self sustaining role in embryo development and the utility of PIF- ELISA to detect viable embryos in non-invasive manner. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4832286 Sat, 14 May 2011 23:00:00 +0100 4832286 http://www.medworm.com/index.php?rid=4798180&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F62 Background: Although chicken oviduct is a useful model and target tissue for reproductive biology and transgenesis, little is known because of the highly specific hormonal regulation and the lack of fundamental researches, including lectin-binding activities and glycobiology. Because lectin is attached to secreted glycoproteins, we hypothesized that lectin could be bound to secretory egg-white proteins, and played a crucial role in the generation of egg white protein in the oviduct. Hence, the purpose of this study was to investigate the structural, histological and lectin-binding characteristics of the chicken oviductal magnum from juvenile and adult hens. Methods: The oviductal magnums from juvenile and adult hens were prepared for ultrastructural analysis, qRT-PCR and immunostaining. Im... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4798180 Sat, 07 May 2011 23:00:00 +0100 4798180 http://www.medworm.com/index.php?rid=4783151&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F60 Background: Single blastocyst transfer has the advantage of maximizing the fresh single pregnancy rate. However, in patients with a low number of good quality embryos on day 3, it remains unclear whether immediate embryo transfer or further embryo culture with blastocyst transfer is the most preferable option. Methods: A retrospective cohort study was carried out in which the outcome of 590 fresh in vitro fertilization (IVF) cycles over a 15 months period and their cryo cycles were analyzed. A total of 341 patients cycles had an elective day 5 strategy independent of intermediate embryo evaluation while another 249 patients underwent a day 5 embryo transfer only if at least four embryos were available on day 3. Blastocyst vitrification was performed using a closed high security system. Re... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4783151 Wed, 04 May 2011 23:00:00 +0100 4783151 http://www.medworm.com/index.php?rid=4764070&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F59 ObjectivesIt has been suggested that inhibin secretion is altered in women with the polycystic ovary syndrome (PCOS). However, the contribution of a preceding luteal phase has not been taken into account. The aim of the present study was to investigate whether progesterone in the context of a simulated luteal phase affects basal and FSH-induced inhibin secretion in women with PCOS and elevated LH. Methods: Ten women with PCOS and 8 normally cycling women participated in an experimental procedure (Exp) involving the administration of a single injection of recombinant FSH (450 IU sc). In the women with PCOS, the procedure was performed before (Exp 1) and after a 20-day treatment with progesterone (Exp 2), while in the normal women on day 2 of the cycle (Exp 3). Inhibin A and B levels were me... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4764070 Thu, 28 Apr 2011 23:00:00 +0100 4764070 http://www.medworm.com/index.php?rid=4759560&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F58 We compared two methods of zona pellucida drilling. 213 embryos were biopsied with acid Tyrode. Each biopsy took 3 minutes and the entire procedure ~29 minutes. 5% of blastomeres lysed, 49% of embryos became blastocyst and 36% of patients became pregnant. 229 embryos were biopsied with laser. Each biopsy took 30 seconds and the entire procedure ~7 minutes. 2.5% of blastomeres lysed, 50.6% of embryos became blastocyst and 47% of patients became pregnant. We can conclude that laser can be used for embryo biopsy. Reduction of embryo exposure and of the number of removed blastomeres is associated with increased blastocysts available for transfer and a better clinical outcome. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4759560 Wed, 27 Apr 2011 23:00:00 +0100 4759560 http://www.medworm.com/index.php?rid=4755166&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F57 Conclusions: There were no strong associations between any adult circulating concentration of sex hormone or SHGB and 2D:4D. These results contribute to the growing body of evidence indicating that 2D:4D is unrelated to adult sex hormone concentrations. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4755166 Tue, 26 Apr 2011 23:00:00 +0100 4755166 http://www.medworm.com/index.php?rid=4748989&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F55 Conclusion: The general aspect of the fetal membranes in Sigmodontinae resembled that found in other cricetid rodents. Compared to murid rodents there were larger numbers of giant cells and in some genera these were seen to congregate at the periphery of the placental disk. Glycogen cells were found to invade the decidua but we did not identify trophoblast in the walls of the deeper decidual arteries. In contrast these vessels were surrounded by large numbers of uNK cells. This survey of wild-trapped specimens from five genera is a useful starting point for the study of placentation in an important subfamily of South American rodents. We note, however, that some of these rodents can be captive bred and recommend that future studies focus on the study of time dated pregnancies. (Source: Rep... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4748989 Sun, 24 Apr 2011 23:00:00 +0100 4748989 http://www.medworm.com/index.php?rid=4748988&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F56 Conclusion: These findings suggest a physiologic role for PMP22 on the expression of alpha 6 integrin. We predict that this may be important for the maintainence of endometrial integrity and to the disease biology associated with altered levels of alpha 6 integrin expression in the endometrium. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4748988 Sun, 24 Apr 2011 23:00:00 +0100 4748988 http://www.medworm.com/index.php?rid=4742983&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F53 Conclusions: The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4742983 Fri, 22 Apr 2011 23:00:00 +0100 4742983 http://www.medworm.com/index.php?rid=4742982&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F54 Conclusion: This study demonstrated that myocardial contractility and expression of key Ca2+ handling proteins were preserved in the early phase and reduced at long-term during OSH deprivation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4742982 Fri, 22 Apr 2011 23:00:00 +0100 4742982 http://www.medworm.com/index.php?rid=4726018&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F52 Conclusion: Our findings demonstrate the presence and hormonal regulation of four different cx transcripts in the salmon ovary. Differences in the spatiotemporal expression profile and hormonal regulation of these cx transcripts likely relate to their different roles during ovarian follicle differentiation and development. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4726018 Mon, 18 Apr 2011 23:00:00 +0100 4726018 http://www.medworm.com/index.php?rid=4720498&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F49 Conclusions: Administration of AMA resulted in attenuation of differentiation, enhancement in apoptosis and loss of viability in early placental villi trophoblast cells in primary culture. Thus, it appears that administration of alpha-helical AMP may adversely affect the process of placentation and pregnancy outcome. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4720498 Fri, 15 Apr 2011 23:00:00 +0100 4720498 http://www.medworm.com/index.php?rid=4720497&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F50 Conclusions: Our results demonstrate that PC-3 and HeLa cancer cells require tNASP to maintain high levels of HSPA2 activity and therefore viability, while PWR-1E cells are unaffected by tNASP depletion. These different cellular responses most likely arise from changes in the interaction between tNASP and HSPA2 and disturbed tNASP chaperoning of linker histones. This study has demonstrated that tNASP is critical for the survival of prostate cancer cells and suggests that targeting tNASP expression can lead to a new approach for prostate cancer treatment. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4720497 Fri, 15 Apr 2011 23:00:00 +0100 4720497 http://www.medworm.com/index.php?rid=4720496&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F51 Conclusions: In utero fetoplacental Vegfr2 gene activity was monitored longitudinally in a quantitative manner using a luciferase reporter gene and bioluminescent imaging during the latter third of gestation. This study demonstrates the feasibility of using the Vegfr2-luc mouse to monitor late gestation fetoplacental angiogenic activity under normal and experimental conditions. Additionally, neonatal Vegfr2 gene activity was monitored for three weeks postpartum, allowing continuous monitoring of Vegfr2 activity during the latter third of gestation and postnatal development within the same animals. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4720496 Fri, 15 Apr 2011 23:00:00 +0100 4720496 http://www.medworm.com/index.php?rid=4715904&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F47 Conclusions: Our results support the evidence that oxidative stress plays a key role in inducing DNA damage; but nuclear alterations and malondialdehyde don't seem to be synchronous. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4715904 Wed, 13 Apr 2011 23:00:00 +0100 4715904 http://www.medworm.com/index.php?rid=4710631&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F46 Conclusions: TRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4710631 Wed, 13 Apr 2011 23:00:00 +0100 4710631 http://www.medworm.com/index.php?rid=4698909&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F45 Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4698909 Sun, 10 Apr 2011 23:00:00 +0100 4698909 http://www.medworm.com/index.php?rid=4686966&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F43 Conclusions: These findings demonstrate that embryo implantation in the rabbit is closely associated with dynamic expression of uterine PC5/6, and that the rabbit may be an appropriate model to investigate the function of PC5/6 in the uterine epithelium during embryo attachment. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4686966 Tue, 05 Apr 2011 23:00:00 +0100 4686966 http://www.medworm.com/index.php?rid=4686965&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F44 Since publication of our article [Amelie Fassbender et al, TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis. 2010, 8:123], we have realised that we did not include the full data in Table One and missed the word (TOF) in the result/discussion section. We have provided here the adapted sentence and another version of the table, including all the information intended. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4686965 Tue, 05 Apr 2011 23:00:00 +0100 4686965 http://www.medworm.com/index.php?rid=4659652&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F41 In the present study we analyzed the existence of asymmetry in the secretion of steroid hormones in pre-pubertal female rats treated with unilateral ovariectomy (ULO) or unilateral perforation of the abdominal wall (sham-surgery). Treated rats were sacrificed at different times after surgery. Since sham-surgery had an apparent effect on the age of first vaginal estrous (FVE) and serum levels hormone, the results of the sham surgery groups were used to assess the effects of their respective surgery treatment groups. On the day of FVE, compensatory ovulation (CO) and compensatory ovarian hypertrophy (COH) were similar in animals with ULO, regardless of the ovary remaining in situ. In ULO treated animals, progesterone (P4) levels were higher than in animals with sham-surgery one hour after tr... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4659652 Tue, 29 Mar 2011 23:00:00 +0100 4659652 http://www.medworm.com/index.php?rid=4654619&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F40 Conclusions: Our data suggest that expression of bovine NPM2 is temporally regulated during early embryogenesis and miR-181a may play a role in its regulation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4654619 Mon, 28 Mar 2011 23:00:00 +0100 4654619 http://www.medworm.com/index.php?rid=4632575&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F39 Conclusions: At community level, widespread screening of Rotterdam criteria will increase the estimated prevalence of PCOS over twofold. Establishing an explicit and contemporaneous method for definition and screening of each PCOS criteria has important investigational implications and increase the comparability of published research. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4632575 Fri, 25 Mar 2011 00:00:00 +0100 4632575 http://www.medworm.com/index.php?rid=4623351&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F38 Conclusions: The SERPINE2 protein is highly expressed in the endometrium during the secretory phase, indicating that it may participate in tissue remodeling involved in implantation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4623351 Wed, 23 Mar 2011 00:00:00 +0100 4623351 http://www.medworm.com/index.php?rid=4623352&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F37 Conclusions: We did not find an association between sperm morphology and clinical outcomes. The impact of teratozoospermia may be masked in ICSI cycles where fertilization, implantation rate and clinical pregnancy rate are the primary outcome measures. However, by examining the timeline of development, we were better able to discern a potential paternal effect at critical transition points from fertilization through activation. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4623352 Tue, 22 Mar 2011 00:00:00 +0100 4623352 http://www.medworm.com/index.php?rid=4617142&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F36 Conclusion: Recent advances in proteomic techniques have provided insight into sperm function and dysfunction. Several multidimensional separation techniques can be utilized to identify and characterize spermatozoa. Future developments in bioinformatics can further assist researchers in understanding the vast amount of data collected in proteomic studies. Moreover, such advances in proteomics may help to decipher metabolites which can act as biomarkers in the detection of sperm impairments and to potentially develop treatment for infertile couples.Further comprehensive studies on sperm-specific proteome, mechanisms of protein function and its proteolytic regulation, biomarkers and functional pathways, such as oxidative-stress induced mechanisms, will provide better insight into physiologic... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4617142 Tue, 22 Mar 2011 00:00:00 +0100 4617142 http://www.medworm.com/index.php?rid=4617143&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F35 Conclusions: Functional KATP channels are expressed in human pregnant myometrium. Down-regulation of Kir6.1 and Kir6.2 expression in myometrium may contribute to the enhanced uterine contractility associated with the onset of labour. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4617143 Mon, 21 Mar 2011 00:00:00 +0100 4617143 http://www.medworm.com/index.php?rid=4607023&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F34 The present study analyzed the participation of the left and right superior ovarian nerves (SON) in regulating progesterone, testosterone, and estradiol serum levels in unilaterally ovariectomized rats on each day of the estrous cycle. For this purpose, ovarian hormone concentrations in serum were measured in animals with eithersham-surgery, unilateral ovariectomy (ULO), unilateral sectioning of the SON, or sectioning of the SON innervation of the in situ ovary in rats with ULO.This investigation results show that the right and left ovaries have different capacities to maintain normal hormone levels, that such capacity varies during the estrous cycle, and that it depends on the integrity of the SON innervation. In rats with only one ovary, the effects of ovarian denervation on hormone leve... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4607023 Fri, 18 Mar 2011 00:00:00 +0100 4607023 http://www.medworm.com/index.php?rid=4595739&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F33 Conclusions: Our result showed for the first time BMP15 and GDF9 expression in bovine cumulus cells. BMP15 and GDF9 mRNA expression in oocytes and cumulus cells was different in calves and cows. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4595739 Tue, 15 Mar 2011 00:00:00 +0100 4595739 http://www.medworm.com/index.php?rid=4562026&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F30 This study aimed to investigate the expression of NGF and its two receptors in uteri and dorsal root ganglia (DRG) in an adenomyosis mouse model, as well as their relationship with the severity of adenomyosis. Methods: Forty newborn ICR mice were randomly divided into the adenomyosis model group and control group (n=20 in each group). Mice in the adenomyosis model group were orally dosed with 2.7 mumol/kg tamoxifen on days 2-5 after birth. Experiments were conducted to identify the expression of NGF- beta and its receptors, tyrosine kinase receptor (trkA) and p75 neurotrophin receptor (p75NTR), in the uterus and DRG in four age groups (90+/-5 d, 140+/-5 d, 190+/-5 d and 240+/-5 d; n=5 mice in each group) by western bolt, immunochemistry and real time reverse transcription-polymerase chain ... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4562026 Tue, 08 Mar 2011 00:00:00 +0100 4562026 http://www.medworm.com/index.php?rid=4562025&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F31 Conclusions: This is the first report showing pregnancy in the domestic cattle following AI with frozen-thawed European bison spermatozoa collected post-mortem. The protocol of spermatozoa collection, dilution, and cryopreservation presented in this paper may be useful for the creating genetic resource bank in the European bison. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4562025 Tue, 08 Mar 2011 00:00:00 +0100 4562025 http://www.medworm.com/index.php?rid=4552656&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F29 Conclusions: Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4552656 Sun, 06 Mar 2011 00:00:00 +0100 4552656 http://www.medworm.com/index.php?rid=4536980&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F28 Human endometrium resists embryo implantation except during the 'window of receptivity'. A change in endometrial gene expression is required for the development of receptivity. Uterine calbindin-D28k (CaBP-28k) is involved in the regulation of endometrial receptivity by intracellular Ca2+. Currently, this protein is known to be mainly expressed in brain, kidneys, and pancreas, but potential role(s) of CaBP-28k in the human uterus during the menstrual cycle remain to be clarified. Thus, in this study we demonstrated the expression of CaBP-28k in the human endometrium in distinct menstrual phases. During the human menstrual cycle, uterine expression levels of CaBP-28k mRNA and protein increased in the proliferative phase and fluctuated in these tissues, compared with that observed in other p... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4536980 Wed, 02 Mar 2011 00:00:00 +0100 4536980 http://www.medworm.com/index.php?rid=4513677&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F27 Conclusions: TNFalpha and IFNgamma modulate EnCL-1 cell function. Moreover, established EnCL-1 cell line appears to be a good model for investigating the molecular mechanisms related to cytokines action and aa metabolites production in cattle. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4513677 Thu, 24 Feb 2011 00:00:00 +0100 4513677 http://www.medworm.com/index.php?rid=4484721&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F25 In the last decades, several steps have been made aiming at rendering human IVF more successful on one side, more tolerable on the other side. The "mild" ovarian stimulation approach, in which a lower-than-average dose of exogenous gonadotropins is given and gonadotropin treatment is started from day 2 to 7 of the cycle, represents a significant step toward a more patient's friendly IVF. However, a clear view of its virtues and defects is still lacking, because only a few prospective randomized trials comparing "mild" vs. conventional stimulation exist, and they do not consider some important aspects, such as, e.g., thawing cycles. This review gives a complete panorama of the "mild" stimulation philosophy, showing its advantages vs. conventional ovarian stimulation, but also discussing its... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4484721 Wed, 16 Feb 2011 00:00:00 +0100 4484721 http://www.medworm.com/index.php?rid=4461121&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F24 The objective of the present study was to isolate and purify the protein fraction(s) of llama seminal plasma responsible for the ovulation-inducing effect of the ejaculate. Methods: Semen collected from male llamas by artificial vagina was centrifuged and the seminal plasma was harvested and stored frozen. Seminal plasma was thawed and loaded onto a Type 1 macro-prep ceramic hydroxylapatite column and elution was carried out using a lineal gradient with 350 mM sodium phosphate. Three protein fractions were identified clearly (Fractions A, B, and C), where a prominent protein band with a mass of 14 kDa was identified in Fraction C. Fraction C was loaded into a sephacryl gel filtration column for further purification using fast protein liquid chromatography (FPLC). Isocratic elution resulted... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4461121 Thu, 10 Feb 2011 00:00:00 +0100 4461121 http://www.medworm.com/index.php?rid=4442887&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F23 Though a widely utilized term and clinical concept, ovarian reserve (OR) has been only inadequately defined. Based on Medline and PubMed searches we here define OR in its various components, review genetic control of OR, with special emphasis on the FMR1 gene, and discuss whether diminished OR (DOR) is treatable. What is generally referred to as OR reflects only a small portion of total OR (TOR), a pool of growing (recruited) follicles (GFs) at different stages of maturation. Functional OR (FOR) depends on size of the follicle pool at menarche and the follicle recruitment rate. Both vary between individuals and, at least partially, are under genetic control. The FMR1 gene plays a role in defining FOR at all ages. Infertility treatments have in the past almost exclusively only centered on t... Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4442887 Mon, 07 Feb 2011 00:00:00 +0100 4442887 http://www.medworm.com/index.php?rid=4437224&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F22 Conclusions: The patterns of expression of these SLRPs in the uterine tissues were found to be hormone-dependent and uterine compartment-related. These results reinforce the existence of subpopulations of endometrial fibroblasts, localized into distinct functional uterine compartments, resembling the organization into basal and functional layers of the human endometrium. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4437224 Fri, 04 Feb 2011 00:00:00 +0100 4437224 http://www.medworm.com/index.php?rid=4429960&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F20 Conclusion: These results taken together suggest that P4 regulates, directly or indirectly, expression of a number of genes involved in the CL structure and function. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4429960 Thu, 03 Feb 2011 00:00:00 +0100 4429960 http://www.medworm.com/index.php?rid=4429961&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F19 Conclusion: The slow oocyte freezing and thawing is a valuable method when no or insufficient number of sperm are available on the day of in vitro fertilization. The quality of sperm is an important factor affecting the clinical outcome achieved by frozen-thawed oocytes. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4429961 Wed, 02 Feb 2011 00:00:00 +0100 4429961 http://www.medworm.com/index.php?rid=4420273&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F18 Conclusions: Our results show that BMP4 is implicated in bovine oocytes maturation and embryo development. Moreover, our findings demonstrate, for the first time, that a correct balance of BMP signaling is needed for proper pre-implantation development of bovine embryos. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4420273 Tue, 01 Feb 2011 00:00:00 +0100 4420273 http://www.medworm.com/index.php?rid=4407087&cid=s_33022_15_f&fid=33022&url=http%3A%2F%2Fwww.rbej.com%2Fcontent%2F9%2F1%2F17 Since publication of our article Spermatogonial stem cell sensitivity to capsaicin: an in vitro study. Reprod Biol Endocrinol 2008, 6:52., we have realised that the legend of Figure 1 needs to be corrected and should read as follows:"Photomicrograph of Gc-5spg and Gc-6spg stained with an anti-activated caspase 3 antibody and counterstained with Haemaluin as described in Materials and Methods. A, Gc-5spg culture treated with 200 uM CAP for 24 h B, Gc-6spg culture treated with 250 uM CAP during 48 h."We apologise for any confusion this may have caused. (Source: Reproductive Biology and Endocrinology) Reproductive Biology and Endocrinology journals http://www.medworm.com/rss/comments.php?id=4407087 Fri, 28 Jan 2011 00:00:00 +0100 4407087