The Journal of Gene Medicine via MedWorm.com MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'The Journal of Gene Medicine' source. Thu, 09 Feb 2012 09:43:48 +0100 Lentiviral vector followed by protein immunisation breaks tolerance against the self‐antigen Her1 and results in lung cancer immunotherapy http://www.medworm.com/index.php?rid=5615650&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2606 ConclusionsThe ability of this vaccine protocol to break both T cell and B cell tolerance to a self‐antigen likely explains its effectiveness. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5615650 Thu, 19 Jan 2012 05:00:00 +0100 5615650 Expression levels of the PiT2 receptor explain, in part, the gestational age‐dependent alterations in transduction efficiency following in Utero retroviral‐mediated gene transfer http://www.medworm.com/index.php?rid=5615649&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2607 ConclusionThe present studies explain the age‐related differences we previously observed in transduction efficiency following in utero GT. They also suggest it may be possible to achieve relatively selective GT to specific tissues by performing in utero GT when levels of PiT2 are maximal in the desired target organ. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5615649 Thu, 19 Jan 2012 05:00:00 +0100 5615649 Electric pulses augment reporter gene expression in the beating heart http://www.medworm.com/index.php?rid=5615648&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2603 AbstractGene therapy of the heart has been attempted in a number of clinical trials with the injection of naked DNA but quantitative information on myocellular transfection rates is not available. The aim of our study was to quantify the efficacy of electropulsing protocols that differ in pulse duration and number to stimulate transfection of cardiomyocytes and to determine the impact on myocardial integrity.Reporter plasmid for constitutive expression of green fluorescent protein (GFP) was injected into the left ventricle of beating hearts of adult, male Lewis rats. Four electrotransfer protocols consisting of repeated long pulses [8 × 20‐msec], trains of short pulses [8 trains of either 60 or 80 × 100‐µsec], or their combination, were compared to control procedures concern... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5615648 Thu, 19 Jan 2012 05:00:00 +0100 5615648 Chimeric antigen receptors for T cell immunotherapy: current understanding and future direction http://www.medworm.com/index.php?rid=5615647&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2604 ConclusionIn conclusion CAR modified T cell therapy is a highly promising treatment for cancer having already demonstrated both promising pre‐clinical and clinical results. However, further modification and additional clinical trials will need to be conducted to ultimately optimize the anti‐tumor efficacy of this approach. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5615647 Thu, 19 Jan 2012 05:00:00 +0100 5615647 CD40‐targeted adenoviral cancer vaccines: the long and winding road to the clinic http://www.medworm.com/index.php?rid=5575513&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1648 SummaryThe ability of Dendritic Cells (DC) to orchestrate innate and adaptive immune responses has been exploited to develop potent anti‐cancer immunotherapies. Recent clinical trials exploring the efficacy of ex vivo modified autologous DC‐based vaccines have reported some promising results. However, in vitro generation of autologous DC for clinical administration, their loading with tumor associated antigens (TAA) and their activation, is laborious and expensive, and, due to interindividual variability in the personalized vaccines, poorly standardized. An attractive alternative approach is to load resident DC in vivo by targeted delivery of TAA, using viral vectors and activating them simultaneously. To this end we have constructed genetically modified Adenoviral (Ad) vectors and bis... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575513 Sat, 07 Jan 2012 05:00:00 +0100 5575513 Antitumor effect of chondroitin sulfate‐coated ternary GM‐CSF plasmid complex for ovarian cancer http://www.medworm.com/index.php?rid=5575512&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1647 ConclusionsThese findings suggest that GM‐CSF/PEI coated with 10‐kilodalton chondroitin sulfate has potential for use in gene therapy of ovarian cancer. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575512 Sat, 07 Jan 2012 05:00:00 +0100 5575512 Oligonucleotide‐mediated gene editing is underestimated in cells expressing mutated GFP, and is positively associated with target protein expression http://www.medworm.com/index.php?rid=5575511&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1639 ConclusionsOur data establish that ssODN‐mediated gene editing is underestimated in cultured mammalian cells expressing non‐fluorescent mutated EGFP, because of variable expression of this mEGFP target gene in the cell population. This conclusion was endorsed by studies in HEK293T‐mEGFP and HepG2‐mEGFP cells. We infer that oligonucleotide‐directed editing of endogenous genes is feasible, particularly for those transcriptionally active. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575511 Sat, 07 Jan 2012 05:00:00 +0100 5575511 Intrathymic injection of lentiviral vector curtails the immune response in the periphery of normal mice http://www.medworm.com/index.php?rid=5575510&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1650 Discussion: Our results show that injection of the LvHA lentiviral vector significantly curbed the immune response to the same antigen in the periphery. Increased selection of HA‐specific regulatory T cells and negative selection of HA‐specific CD8+ T‐cell precursors may explain the results. Our results establish the feasibility of IT injections of lentiviral vectors to manipulate T‐cell tolerance in the thymus of normal mice, for basic and pre‐clinical research. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575510 Sat, 07 Jan 2012 05:00:00 +0100 5575510 CCL19 (ELC) improves TH1‐polarized immune responses and protective immunity in a murine Her2/neu DNA vaccination model http://www.medworm.com/index.php?rid=5575516&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1651 ConclusionsCCL19 is able to induce a TH‐1 polarization of the anti Her2/neu immune response which can be further amplified by GM‐CSF. Clinical use of a pDNA(Her2/neu‐CCL19 ± GM‐CSF) vaccine might be promising in Her2/neu + breast cancer in the clinical situation of minimal residual disease. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575516 Fri, 06 Jan 2012 05:00:00 +0100 5575516 CHM/REP1 cDNA delivery by lentiviral vectors provides functional expression of the transgene in the RPE of choroideremia mice http://www.medworm.com/index.php?rid=5575515&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1652 ConclusionsLentiviral CHM/REP1 cDNA transgene rescues the prenylation defect in CHM mouse RPE and thus could be used to restore REP1 activity in the RPE of CHM patients. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575515 Fri, 06 Jan 2012 05:00:00 +0100 5575515 Enhanced intracellular delivery and multi‐target gene silencing triggered by tripodal RNA structures http://www.medworm.com/index.php?rid=5575514&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1653 ConclusionsOur data provide the example of synthetic multifunctional RNA structures that enable multiple gene interference in mammalian cells, which could become powerful tools for the efficient combinatorial iRNA strategy. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575514 Fri, 06 Jan 2012 05:00:00 +0100 5575514 Endothelium‐specific over expression of human VEGF‐D in mice leads to increased tumor frequency and a reduced lifespan http://www.medworm.com/index.php?rid=5633819&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2608 ConclusionsIt is concluded that targeted unregulated long‐term expression of hVEGF‐D in endothelium may not be useful and reduces life span of the transgenic mice. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5633819 Sun, 01 Jan 2012 05:00:00 +0100 5633819 A syringe‐focused ultrasound device for simultaneous injection of DNA and gene transfer http://www.medworm.com/index.php?rid=5623736&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1633 ConclusionsThis new technique provides a simple, safe and efficient nonviral gene delivery approach in skeletal muscle and shows promising applications for gene therapy of human disease, such as Alzheimer's disease. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5623736 Sun, 01 Jan 2012 05:00:00 +0100 5623736 Pro‐opiomelanocortin gene delivery suppresses the growth of established Lewis lung carcinoma through a melanocortin‐1 receptor‐independent pathway http://www.medworm.com/index.php?rid=5623735&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1625 ConclusionsThese results support the existence of an MC‐1R‐independent pathway for POMC gene therapy, which further expands the therapeutic spectrum of POMC therapy for multiple types of cancer. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5623735 Sun, 01 Jan 2012 05:00:00 +0100 5623735 Baculovirus‐mediated vascular endothelial growth factor‐DΔNΔC gene transfer induces angiogenesis in rabbit skeletal muscle http://www.medworm.com/index.php?rid=5623734&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1637 ConclusionsWe show the first evidence of biologically significant baculoviral gene transfer in skeletal muscle of NZW rabbits using VEGF‐DΔNΔC as a therapeutic transgene. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5623734 Sun, 01 Jan 2012 05:00:00 +0100 5623734 In this issue http://www.medworm.com/index.php?rid=5623733&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2602 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5623733 Sun, 01 Jan 2012 05:00:00 +0100 5623733 Issue Information http://www.medworm.com/index.php?rid=5623732&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2590 No abstract is available for this article. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5623732 Sun, 01 Jan 2012 05:00:00 +0100 5623732 Pro‐opiomelanocortin gene delivery suppresses the growth of established Lewis lung carcinoma through Melanocortin‐1 receptor‐independent pathway http://www.medworm.com/index.php?rid=5481665&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1625 ConclusionsThese results support the existence of an MC‐1R‐independent pathway for POMC gene therapy, which further expands the therapeutic spectrum of POMC therapy for multiple types of cancer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5481665 Tue, 06 Dec 2011 05:00:00 +0100 5481665 Highly potent mRNA based cancer vaccines represent an attractive platform for combination therapies supporting an improved therapeutic effect http://www.medworm.com/index.php?rid=5615646&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.2605 AbstractDirect vaccination with messenger RNA (mRNA) encoding tumor antigens is a novel and promising approach in cancer immunotherapy. CureVac's mRNA vaccines contain free and protamine‐complexed mRNA. Such two‐component mRNA vaccines support both: antigen expression and immune stimulation. These self‐adjuvanting RNA vaccines, administered intradermaly without any additional adjuvant, induce a comprehensive balanced immune response, comprising antigen specific CD4+ T cells, CD8+ T cells and B cells. The balanced immune response results in a strong anti‐tumor effect and complete protection against antigen positive tumor cells. This tumor inhibition elicited by mRNA vaccines is due to the concerted action of different players. After just two intradermal vaccinations we observe multi... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5615646 Thu, 01 Dec 2011 05:00:00 +0100 5615646 EphrinA1‐EphA2 interaction‐mediated apoptosis and Flt3L‐induced immunotherapy inhibits tumor growth in a breast cancer mouse model http://www.medworm.com/index.php?rid=5575509&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1649 ConclusionsThe results indicating induction of apoptosis and inhibition of mammary tumor growth show the potential therapeutic benefits of HAd‐EphrinA1‐Fc. In combination with HAd‐Flt3L, this represents a promising strategy to effectively induce mammary tumor regression by HAd vector‐based therapy. Copyright © 2012 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5575509 Thu, 01 Dec 2011 05:00:00 +0100 5575509 Targeted expression of Escherichia coli purine nucleoside phosphorylase and Fludara® for prostate cancer therapy http://www.medworm.com/index.php?rid=5541611&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1620 ConclusionsThe ARR2PB‐PNP/Fludara system induced massive tumor cell death and a prolonged life span without systemic cytotoxicity; therefore, it might be a more attractive strategy for suicide gene therapy of prostate cancer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5541611 Thu, 01 Dec 2011 05:00:00 +0100 5541611 RGD peptide‐modified adenovirus expressing hepatocyte growth factor and X‐linked inhibitor of apoptosis improves islet transplantation http://www.medworm.com/index.php?rid=5541610&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1626 ConclusionsThese results indicated that ex vivo transduction of islets with RGD‐Adv‐hHGF‐hXIAP decreased apoptotic islet cell death and improved islet revascularization, and eventually might improve the outcome of human islet transplantation. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5541610 Thu, 01 Dec 2011 05:00:00 +0100 5541610 High‐capacity adenoviral vectors circumvent the limitations of ΔE1 and ΔE1/ΔE3 adenovirus vectors to induce multispecific transgene product‐directed CD8 T‐cell responses http://www.medworm.com/index.php?rid=5541609&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1629 ConclusionsDe novo expression of viral genes from ΔE1Ad vector genomes restricts the multispecificity of transgene product‐specific CTLs by immunodominance effects. HC‐Ad vectors devoid of Ad genes are favorable for the induction of both multispecific CD8 T‐cell responses and high antibody responses. Our results suggest the deletion of Ad genes as an important means for developing potent Ad‐based vectored vaccines. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5541609 Thu, 01 Dec 2011 05:00:00 +0100 5541609 In this issue http://www.medworm.com/index.php?rid=5541608&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1642 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5541608 Thu, 01 Dec 2011 05:00:00 +0100 5541608 Editorial Board Article http://www.medworm.com/index.php?rid=5541607&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1640 No abstract is available for this article. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5541607 Thu, 01 Dec 2011 05:00:00 +0100 5541607 The assessment of adeno‐associated vectors as potential intrinsic treatments for brainstem axon regeneration http://www.medworm.com/index.php?rid=5436144&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1628 ConclusionsSerotype 2 AAV‐EGFP enabled easy visualization of brainstem axon regeneration. Rigorous models of axonal injury (complete transection and cell implantation), should be used in combination with AAV‐EGFP, to directly assess AAV mediated expression of therapeutic transgenes as intrinsic treatments to improve axonal regeneration. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5436144 Tue, 22 Nov 2011 05:00:00 +0100 5436144 ΔE1 and high‐capacity adenoviral vectors expressing full‐length codon‐optimized merozoite surface protein 1 for vaccination against Plasmodium falciparum http://www.medworm.com/index.php?rid=5415606&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1627 ConclusionsOur findings suggest that Ad vectors expressing full‐length codon‐optimized MSP‐1 are promising candidate vaccines against Plasmodium falciparum infections. Use of the HC‐Ad vector type for delivery and the CAG promoter to control MSP‐1 expression may further increase efficacy of this vaccine candidate. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5415606 Wed, 16 Nov 2011 05:00:00 +0100 5415606 High‐capacity adenoviral vectors circumvent the limitations of ΔE1 and ΔE1/ΔE3 Ad vectors to induce multispecific transgene product‐directed CD8 T cell responses http://www.medworm.com/index.php?rid=5415605&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1629 ConclusionsDe novo expression of viral genes from ΔE1Ad vector genomes restricts the multispecificity of transgene product‐specific CTLs by immunodominance effects. HC‐Ad vectors devoid of adenovirus genes are favorable for the induction of both multispecific CD8 T cell responses and high antibody responses. Our results suggest deletion of adenovirus genes as an important means to develop potent adenovirus‐based vectored vaccines. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5415605 Wed, 16 Nov 2011 05:00:00 +0100 5415605 Targeted expression of herpes simplex virus thymidine kinase in adenovirus‐infected cells reduces virus titers upon treatment with ganciclovir in vitro http://www.medworm.com/index.php?rid=5531277&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1638 ConclusionsWe extended the concept of enzyme/prodrug therapy to adenovirus infections by selectively sensitizing adenovirus‐infected cells to treatment with GCV. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5531277 Tue, 01 Nov 2011 04:00:00 +0100 5531277 Baculovirus‐mediated VEGF‐DΔNΔC Gene Transfer Induces Angiogenesis In Rabbit Skeletal Muscle http://www.medworm.com/index.php?rid=5499894&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1637 ConclusionsWe show the first evidence of biologically significant baculoviral gene transfer in skeletal muscle of NZW rabbits using VEGF‐DΔNΔC as a therapeutic transgene. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5499894 Tue, 01 Nov 2011 04:00:00 +0100 5499894 Characterisation of enzyme prodrug gene therapy combinations in coated spheroids and vascular networks in vitro http://www.medworm.com/index.php?rid=5481664&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1635 ConclusionsCollectively these data demonstrate that cytotoxic rather than cytostatic activity is necessary for efficient vascular disruption in vitro, and bystander killing is not essential. We identify NTR/CB1954 and NTR/metronidazole as candidates for in vivo investigation of vascular targeted gene therapy. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5481664 Tue, 01 Nov 2011 04:00:00 +0100 5481664 Conference Calendar http://www.medworm.com/index.php?rid=5463703&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1636 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5463703 Tue, 01 Nov 2011 04:00:00 +0100 5463703 The Journal of Gene Medicine Japanese Society of Gene Therapy Young Investigator Award from 2010 http://www.medworm.com/index.php?rid=5463702&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1632 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5463702 Tue, 01 Nov 2011 04:00:00 +0100 5463702 In this issue http://www.medworm.com/index.php?rid=5463701&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1631 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5463701 Tue, 01 Nov 2011 04:00:00 +0100 5463701 Issue Information http://www.medworm.com/index.php?rid=5463700&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1630 No abstract is available for this article. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5463700 Tue, 01 Nov 2011 04:00:00 +0100 5463700 Efficient transgene expression from naked DNA delivered into an arterio‐venous fistula model for kidney dialysis http://www.medworm.com/index.php?rid=5329103&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1615 ConclusionsThese findings open the possibility of vascular transduction with naked DNA bearing therapeutic genes in areas prone to NIH to ameliorate vein graft pathologies using simple and clinically applicable vector delivery methods. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5329103 Mon, 17 Oct 2011 04:00:00 +0100 5329103 Chemical coupling as a potent strategy for preparation of targeted bacteriophage‐derived gene nanocarriers into eukaryotic cells http://www.medworm.com/index.php?rid=5329102&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1617 ConclusionsOur findings highlight chemical coupling as an efficient and straightforward strategy for displaying of a targeting molecule at high density on the phage surface which, in its turn, may improve the efficiency of phage‐mediated gene transfer and expression. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5329102 Mon, 17 Oct 2011 04:00:00 +0100 5329102 Multiple components in serum contribute to hepatic transgene expression by lipoplex in mice http://www.medworm.com/index.php?rid=5329101&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1618 ConclusionsHepatic transgene expression by lipoplex was enhanced by interaction with multiple components in serum. Interaction of lipoplex with serum could be an important factor for successful in vivo gene transfer. Hence, the information obtained in this study is valuable for the future development of effective gene carriers. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5329101 Mon, 17 Oct 2011 04:00:00 +0100 5329101 A syringe Focused Ultrasound Device for Simultaneous Injection of DNA and Gene Transfer http://www.medworm.com/index.php?rid=5446414&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1633 ConclusionsThe new technique provides a simple, safe and efficient nonviral gene delivery approach in skeletal muscle and shows promising applications for gene therapy of human disease, such as Alzheimer's Disease (AD). Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5446414 Sat, 01 Oct 2011 04:00:00 +0100 5446414 Corneal gene delivery: chitosan oligomer as a carrier of CpG rich, CpG free, or S/MAR plasmid DNA http://www.medworm.com/index.php?rid=5436143&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1634 ConclusionsThis study reveals the potential of comparing various plasmids as an approach to enhance transgene expression. The delivery system designed here represents the next step in the development of effective vectors for corneal gene therapy. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5436143 Sat, 01 Oct 2011 04:00:00 +0100 5436143 RGD peptide‐modified adenovirus expressing HGF and XIAP improves islet transplantation http://www.medworm.com/index.php?rid=5415604&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1626 ConclusionThese results indicated that ex vivo transduction of islets with RGD‐Adv‐hHGF‐hXIAP decreased apoptotic islet cell death, improved islet revascularization, and eventually might improve the outcome of human islet transplantation. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5415604 Sat, 01 Oct 2011 04:00:00 +0100 5415604 Conference Calendar http://www.medworm.com/index.php?rid=5353714&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1623 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353714 Sat, 01 Oct 2011 04:00:00 +0100 5353714 Gene delivery to the heart: an updated review on vectors and methods http://www.medworm.com/index.php?rid=5353713&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1619 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353713 Sat, 01 Oct 2011 04:00:00 +0100 5353713 Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model http://www.medworm.com/index.php?rid=5353712&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1603 ConclusionsThese results demonstrate that transfer of the genomic‐cDNA hybrid expressing both isoforms of the HGF gene might provide higher therapeutic effects than the cDNA sequence producing HGF728 alone in the treatment of ischemic heart disease. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353712 Sat, 01 Oct 2011 04:00:00 +0100 5353712 In this issue http://www.medworm.com/index.php?rid=5353711&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1622 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353711 Sat, 01 Oct 2011 04:00:00 +0100 5353711 Issue Information http://www.medworm.com/index.php?rid=5353710&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1621 No abstract is available for this article. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353710 Sat, 01 Oct 2011 04:00:00 +0100 5353710 Differential biodistribution of oncolytic poxvirus administered systemically in an autochthonous model of hepatocellular carcinoma http://www.medworm.com/index.php?rid=5353709&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1624 ConclusionThis study emphasizes the importance of the route of administration of the vector and highlights the relevance of systemic injection of oncolytic vaccinia virus in the context of hepatocellular carcinoma. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5353709 Sat, 01 Oct 2011 04:00:00 +0100 5353709 Thermosensitive Hydrogel for Prolonged Delivery of Lentiviral Vector Expressing Neurotrophin‐3 in vitro http://www.medworm.com/index.php?rid=5266879&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1613 ConclusionsThe system shows promise for in‐situ, thermoresponsive delivery of lentiviral vectors providing long term gene expression for therapeutic factors to treat conditions such as injury to the nervous system. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266879 Mon, 26 Sep 2011 04:00:00 +0100 5266879 Model‐specific selection of molecular targets for heart failure gene therapy http://www.medworm.com/index.php?rid=5256157&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1610 SummaryHeart failure (HF) is a complex multifaceted problem of abnormal ventricular function and structure. In recent years we have accumulated new information allowing for a more detailed understanding of the cellular and molecular alterations that are the underpinnings of diverse causes of HF including myocardial ischemia, pressure‐overload, volume‐overload or intrinsic cardiomyopathy. Modern pharmacological approaches to treat of HF have had a significant impact on the course of the disease, but they do not reverse the underlying pathological state of the heart. Therefore gene‐based therapy holds a great potential as a targeted treatment for cardiovascular diseases. Here we survey the relative therapeutic efficacy of genetic modulation of β‐adrenergic receptor signaling, Ca2+ h... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5256157 Fri, 23 Sep 2011 04:00:00 +0100 5256157 Evidence of DNA Transfer across a Model Membrane by a Neutral Amphiphilic Block Copolymer http://www.medworm.com/index.php?rid=5256156&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1612 ConclusionThese results strongly suggest that gene transfection by neutral block copolymers may involve the formation of cellular pores; in addition, they also highlight that in vivo gene transfection requires the use of adequately soluble block copolymers. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5256156 Fri, 23 Sep 2011 04:00:00 +0100 5256156 Gene delivery methods in cardiac gene therapy http://www.medworm.com/index.php?rid=5256155&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1609 AbstractGene therapy for the treatment of heart failure is emerging as a multidisciplinary field with advancements in identifying key signaling pathways, modernized vector creation and delivery technologies. While these discoveries offer significant progress, selecting optimal methods for the vector delivery remains a key component for efficient cardiac gene therapy to validate the targets in rodent models and to test clinically relevant ones in pre‐clinical models. While the goals of higher transduction efficiency and cardiac specificity can be achieved with several delivery methods, the invasiveness and patient safety remain unclear for clinical application. In this review, we discuss various features of currently available vector delivery methods for cardiac gene therapy. Copyright ©... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5256155 Fri, 23 Sep 2011 04:00:00 +0100 5256155 A phase I clinical study of naked DNA expressing two isoforms of hepatocyte growth factor to treat patients with critical limb ischemia http://www.medworm.com/index.php?rid=5342119&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1614 ConclusionThese results support the performance of a phase II randomized controlled trial with pCK‐HGF‐X7. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5342119 Thu, 01 Sep 2011 04:00:00 +0100 5342119 Targeted expression of E. coli purine nucleoside phosphorylase and Fludara for prostate cancer therapy http://www.medworm.com/index.php?rid=5329100&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1620 ConclusionsThe ARR2PB‐PNP/Fludara system induced massive tumor cell death and prolonged life span without systemic cytotoxicity, therefore it might be a more attractive strategy for suicide gene therapy of prostate cancer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5329100 Thu, 01 Sep 2011 04:00:00 +0100 5329100 Conference Calendar http://www.medworm.com/index.php?rid=5266885&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1607 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266885 Thu, 01 Sep 2011 04:00:00 +0100 5266885 Long‐term functional adeno‐associated virus‐microdystrophin expression in the dystrophic CXMDj dog http://www.medworm.com/index.php?rid=5266884&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1602 ConclusionsThe data obtained in the present study suggest that the use of canine‐specific and mRNA sequence‐optimized microdystrophin genes in conjunction with a muscle‐specific promoter results in high and stable levels of microdystrophin expression in a canine model of DMD. This approach will potentially allow the reduction of dosage and contribute towards the development of a safe and effective AAV gene therapy clinical trial protocol for DMD. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266884 Thu, 01 Sep 2011 04:00:00 +0100 5266884 Non‐integrating lentiviral vectors for specific killing of Epstein–Barr virus nuclear antigen 1‐positive B cell lymphoma cells http://www.medworm.com/index.php?rid=5266883&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1601 ConclusionsTransgene expression from our NILVs is both EBNA1‐specific and dependent upon the presence of the FR. The results obtained in the present study indicate that NILVs have potential use in the treatment of EBV‐associated B cell malignancies. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266883 Thu, 01 Sep 2011 04:00:00 +0100 5266883 The position of the AUG start codon in MFG‐based γ‐retroviral vectors has a dramatic effect on translation‐dependent protein expression http://www.medworm.com/index.php?rid=5266882&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1599 ConclusionsThese finding emphasize the scanning rule for translation initiation and stress the importance of cloning genes of interest into or near the native NcoI start site of MFG‐based retroviral vectors. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266882 Thu, 01 Sep 2011 04:00:00 +0100 5266882 In this issue http://www.medworm.com/index.php?rid=5266881&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1606 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266881 Thu, 01 Sep 2011 04:00:00 +0100 5266881 Issue Information http://www.medworm.com/index.php?rid=5266880&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1605 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266880 Thu, 01 Sep 2011 04:00:00 +0100 5266880 Effect of VEGF gene transfer on infarct size, left ventricular function and myocardial perfusion in sheep after two months of coronary artery occlusion http://www.medworm.com/index.php?rid=5266878&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1608 ConclusionsIn sheep with AMI pVEGF gene transfer affords long term infarct size reduction, yielding regional LV function and perfusion improvement and reducing remodeling progression. These results suggest the potential usefulness of this approach in the clinical setting. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5266878 Thu, 01 Sep 2011 04:00:00 +0100 5266878 Non‐integrating lentiviral vectors for specific killing of Epstein‐Barr virus nuclear antigen 1‐positive B cell lymphoma cells http://www.medworm.com/index.php?rid=5153420&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1601 ConclusionTransgene expression from our NILVs is both EBNA1‐specific and dependent upon the presence of the FR. Results from this study indicate that NILVs have potential use in the treatment of EBV‐associated B cell malignancies. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5153420 Tue, 16 Aug 2011 23:00:00 +0100 5153420 Intracellular trafficking and fate of chimeric adenovirus 5/F35 in human B lymphocytes http://www.medworm.com/index.php?rid=5034569&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1588 ConclusionsTogether, these results suggest that Ad5/F35 is more efficiently transduced in cells with a strong transcriptional activity due to differences in intracellular trafficking. This finding extends our current knowledge of mechanisms of adenovirus‐mediated gene transfer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5034569 Thu, 14 Jul 2011 23:00:00 +0100 5034569 Endonucleases: tools to correct the dystrophin gene http://www.medworm.com/index.php?rid=5256154&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1611 ConclusionsEndonucleases are able to: 1) restore the normal reading of a gene with a frame shift mutation; 2) delete a non‐sense codon and 3) knockout a gene. Endonucleases could thus be used to treat Duchenne Muscular Dystrophy (DMD) and other hereditary diseases due to a nonsense codon or to a frame shift mutation. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5256154 Fri, 01 Jul 2011 04:00:00 +0100 5256154 Lentiviral‐based BMP4 in vivo gene transfer strategy increases pull‐out tensile strength without an improvement in the osteointegration of the tendon graft in a rat model of biceps tenodesis http://www.medworm.com/index.php?rid=5189776&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1604 ConclusionAlthough the LV‐BMP4 in vivo gene transfer strategy did not enhance osteointegration of the tendon graft, it yielded a marked improvement in the return of the pull‐out strength of the tendon graft. This presumably was due largely to the bone formation effect of BMP4 that traps or anchors the tendon graft onto the bony tunnel. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5189776 Thu, 30 Jun 2011 23:00:00 +0100 5189776 Enhanced cardio‐protective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model http://www.medworm.com/index.php?rid=5167584&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1603 ConclusionsThese results demonstrated that transfer of the genomic‐cDNA hybrid expressing both isoforms of the HGF gene might provide higher therapeutic effects than the cDNA sequence producing HGF728 alone in the treatment of ischemic heart diseases. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5167584 Thu, 30 Jun 2011 23:00:00 +0100 5167584 Long‐term functional AAV‐microdystrophin expression in the dystrophic CXMDj dog http://www.medworm.com/index.php?rid=5153419&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1602 ConclusionsOur data suggests that use of a canine‐specific and mRNA sequence‐optimized microdystrophin genes in conjunction with a muscle‐specific promoter results in high and stable levels of microdystrophin expression in a canine model of DMD. This approach will potentially allow reduction of dosage and contribute towards development of a safe and effective AAV gene therapy clinical trial protocol for DMD. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5153419 Thu, 30 Jun 2011 23:00:00 +0100 5153419 The Evolution of Heart Gene Delivery Vectors http://www.medworm.com/index.php?rid=5115598&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1600 AbstractGene therapy holds promise for treating numerous heart diseases. A key premise for the success of cardiac gene therapy is the development of powerful gene transfer vehicles that can achieve highly efficient and persistent gene transfer specifically in the heart. Other features of an ideal vector include negligible toxicity, minimal immunogenicity and easy manufacturing. Rapid progress in the fields of molecular biology and virology has offer great opportunities to engineer various genetic materials for heart gene delivery. Several non‐viral vectors (such as naked plasmids, plasmid lipid/polymer complexes and oligonucleotides) have been tested. Commonly used viral vectors include lentivirus, adenovirus and adeno‐associated virus (AAV). Among these, AAV has shown many attractive ... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5115598 Thu, 30 Jun 2011 23:00:00 +0100 5115598 Conference Calendar http://www.medworm.com/index.php?rid=5075066&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1592 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5075066 Thu, 30 Jun 2011 23:00:00 +0100 5075066 In this issue http://www.medworm.com/index.php?rid=5075065&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1591 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5075065 Thu, 30 Jun 2011 23:00:00 +0100 5075065 Issue Information http://www.medworm.com/index.php?rid=5075064&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1593 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5075064 Thu, 30 Jun 2011 23:00:00 +0100 5075064 The position of the AUG start codon in MFG‐based γ‐retroviral vectors has a dramatic effect on translation dependent protein expression http://www.medworm.com/index.php?rid=5075063&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1599 ConclusionsThese finding emphasize the scanning rule for translation initiation and stress the importance of cloning genes of interest into or near the native Nco I start site of MFG‐based retroviral vectors. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5075063 Thu, 30 Jun 2011 23:00:00 +0100 5075063 Mechanisms of polyethylenimine‐mediated DNA delivery: free carrier helps to overcome the barrier of cell‐surface glycosaminoglycans http://www.medworm.com/index.php?rid=4990378&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1587 ConclusionsFree PEI is essential for minimizing the undesirable binding of polyplexes to cell‐surface GAGs that have negative impact on transfection. The same mechanism may be important in transfections with other polyplexes that require high charge ratios for transfection. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4990378 Wed, 29 Jun 2011 23:00:00 +0100 4990378 Therapeutic ultrasound promotes plasmid DNA uptake by clathrin‐mediated endocytosis http://www.medworm.com/index.php?rid=4990377&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1586 ConclusionsThe present data indicate that the plasmid DNA uptake promoted by US should occur via clathrin‐mediated endocytosis. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4990377 Wed, 29 Jun 2011 23:00:00 +0100 4990377 7th Australasian Gene Therapy Society meeting http://www.medworm.com/index.php?rid=4973915&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1582 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973915 Sun, 26 Jun 2011 23:00:00 +0100 4973915 Gene therapy with adenovirus‐delivered indoleamine 2,3‐dioxygenase improves renal function and morphology following allogeneic kidney transplantation in rat http://www.medworm.com/index.php?rid=4973914&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1584 ConclusionsRGD‐AdTIDO therapy improves renal function and morphology in a clinically relevant model of acute rejection. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973914 Sun, 26 Jun 2011 23:00:00 +0100 4973914 Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in mouse squamous cell carcinoma model http://www.medworm.com/index.php?rid=4930495&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1578 ConclusionThese findings suggest that CBOVS could protect adenoviruses from neutralizing antibodies and systemically deliver them to lung tumors. Further, CBOVS appears to have potential as a tumor cell vaccine that activates cytotoxic immunity against cancer cells. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930495 Mon, 13 Jun 2011 23:00:00 +0100 4930495 Effect of poly(ADP‐ribose) polymerase 1 on integration of the adeno‐associated viral vector genome http://www.medworm.com/index.php?rid=4930494&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1577 ConclusionsOur findings indicate that manipulation of PARP1 activity could be used as a tool for developing new, effective AAV‐based therapies for the treatment of genetic diseases and cancer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930494 Mon, 13 Jun 2011 23:00:00 +0100 4930494 A Single Direct Injection into the Left Ventricular Wall of an AAV9 Vector Expressing EcSOD from the Cardiac Troponin‐T Promoter Protects Mice Against Myocardial Infarction http://www.medworm.com/index.php?rid=4930493&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1576 ConclusionsAAV serotype 9 is highly efficient for cardiac gene delivery, as evidenced by early onset and high‐level gene expression. AAV9 mediated, cardiac selective overexpression of EcSOD from the cTnT promoter significantly reduced infarct size in mice.AAV, gene therapy, cardiac‐selective promoter, myocardial infarction, cardioprotection, EcSOD Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930493 Mon, 13 Jun 2011 23:00:00 +0100 4930493 AAV‐Mediated Expression of Acid Sphingomyelinase Decreases Atherosclerotic Lesion Formation in Apolipoprotein E−/− Mice http://www.medworm.com/index.php?rid=4930492&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1575 ConclusionsUnexpectedly, the lesion area of the entire aorta was actually reduced significantly in the AAV8‐ASM virus‐treated group. Hepatic expression and secretion of ASM into the circulation did not accelerate or exacerbate, but rather decreased lesion formation in ApoE−/− mice. Thus, plasma ASM activity does not appear to be rate limiting for plaque formation during atherogenesis. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930492 Mon, 13 Jun 2011 23:00:00 +0100 4930492 Enhanced delivery of mPEG‐PLGA‐PLL nanoparticles loading PDGF‐BB siRNA by ultrasound and/or microbubbles to rat RPE cells http://www.medworm.com/index.php?rid=4930491&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1574 ConclusionsUS and/or MBs could be used safely to enhance the delivery of NPs loading siRNA to rat RPE‐J cells. A combination of the chemical (mPEG‐PLGA‐PLL NPs loading siRNA) and physical (ultrasound) approaches could more effectively downregulate the mRNA and protein expression of PDGF‐BB. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930491 Mon, 13 Jun 2011 23:00:00 +0100 4930491 Immunity against a therapeutic xenoprotein/Fc construct delivered by gene transfer is reduced through binding to the inhibitory receptor FcγRIIb http://www.medworm.com/index.php?rid=5056605&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1598 ConclusionsThis study shows that inhibitory FcγRIIb receptors interacting with the wild‐type IgG1‐Fc reduce immunity against Ex4/Fc, and suggest an approach for reducing the immunogenicity of therapeutic proteins in the context of gene therapy. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5056605 Tue, 31 May 2011 23:00:00 +0100 5056605 Sleeping Beauty‐mediated correction of Fanconi anemia type C http://www.medworm.com/index.php?rid=5034568&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1589 ConclusionsThe SB transposon system achieved stable expression of therapeutic FA‐C genes, complementing the genetic defect in patient‐derived cells by non‐viral gene transfer. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=5034568 Tue, 31 May 2011 23:00:00 +0100 5034568 Sustained, high transgene expression in liver with plasmid vectors using optimized promoter‐enhancer combinations http://www.medworm.com/index.php?rid=4990376&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1585 AbstractPlasmid based gene therapy approaches often lack long term transgene expression in vivo due to silencing or loss of the vector. One way to overcome these limitations is to combine non‐silenced promoters with strong enhancers. Here we combine murine or human cytomegalovirus (CMV) derived enhancer elements with the human elongation factor 1 alpha (EF1α) promoter in a plasmid backbone devoid of potentially immunostimulating CpG sequences. Luciferase transgene activity was monitored in mouse liver after hydrodynamic plasmid delivery. Luciferase activity of a CMV promoter driven plasmid rapidly declined within days, whereas the activity of the EF1α driven plasmid remained high for two weeks (murine enhancer) and detectable for >80 days (human enhancer). Expression levels clearl... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4990376 Tue, 31 May 2011 23:00:00 +0100 4990376 Conference Calendar http://www.medworm.com/index.php?rid=4973923&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1581 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973923 Tue, 31 May 2011 23:00:00 +0100 4973923 Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model http://www.medworm.com/index.php?rid=4973922&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1578 ConclusionsThese findings suggest that CBOVS could protect adenoviruses from neutralizing antibodies and systemically deliver them to lung tumors. Furthermore, CBOVS appears to have potential as a tumor cell vaccine that activates cytotoxic immunity against cancer cells. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973922 Tue, 31 May 2011 23:00:00 +0100 4973922 A single direct injection into the left ventricular wall of an adeno‐associated virus 9 (AAV9) vector expressing extracellular superoxide dismutase from the cardiac troponin‐T promoter protects mice against myocardial infarction http://www.medworm.com/index.php?rid=4973921&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1576 ConclusionsAAV serotype 9 is highly efficient for cardiac gene delivery, as evidenced by early onset and high‐level gene expression. AAV9‐mediated, cardiac selective overexpression of EcSOD from the cTnT promoter significantly reduced infarct size in mice. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973921 Tue, 31 May 2011 23:00:00 +0100 4973921 Adeno‐associated virus‐mediated expression of acid sphingomyelinase decreases atherosclerotic lesion formation in apolipoprotein E−/− mice http://www.medworm.com/index.php?rid=4973920&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1575 ConclusionsUnexpectedly, the lesion area of the entire aorta was reduced significantly in the AAV8‐ASM virus‐treated group. Hepatic expression and secretion of ASM into the circulation did not accelerate or exacerbate, but rather decreased, lesion formation in ApoE−/− mice. Thus, plasma ASM activity does not appear to be rate limiting for plaque formation during atherogenesis. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973920 Tue, 31 May 2011 23:00:00 +0100 4973920 Enhanced delivery of monomethoxypoly(ethylene glycol)‐poly(lactic‐co‐glycolic acid)‐poly l‐lysine nanoparticles loading platelet‐derived growth factor BB small interfering RNA by ultrasound and/or microbubbles to rat retinal pigment epithelium cells http://www.medworm.com/index.php?rid=4973919&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1574 ConclusionsUS and/or MBs could be used safely to enhance the delivery of NPs loading siRNA to rat RPE‐J cells. A combination of the chemical (mPEG‐PLGA‐PLL NPs loading siRNA) and physical (US) approaches could more effectively downregulate the mRNA and protein expression of PDGF‐BB. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973919 Tue, 31 May 2011 23:00:00 +0100 4973919 The multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster as a therapeutic suicide gene of breast cancer cells http://www.medworm.com/index.php?rid=4973918&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1573 ConclusionsThese results suggest that the Dm‐dNK/nucleoside analog system could be a novel therapeutic strategy for treating breast cancer and improving anti‐tumor efficacy, as well as for optimizing approaches for suicide gene therapy. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973918 Tue, 31 May 2011 23:00:00 +0100 4973918 In this issue http://www.medworm.com/index.php?rid=4973917&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1580 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973917 Tue, 31 May 2011 23:00:00 +0100 4973917 Issue Information http://www.medworm.com/index.php?rid=4973916&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1579 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973916 Tue, 31 May 2011 23:00:00 +0100 4973916 Hepatic Control Elements Promote Long‐term Expression of Human Coagulation Factor IX Gene in Hydrodynamically Transfected Mice http://www.medworm.com/index.php?rid=4973913&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1583 ConclusionsWe have shown in mouse models that hydrodynamic transfection of pBS‐HCRII‐HP‐FIXA and pBS‐HCRI/II‐HP‐FIXA was able to induce and maintain the expression and clotting activity of human factor IX for a long period of time at a potentially therapeutic level. With an appropriate delivery system this type of plasmid vector could be clinically useful for hepatic expression of therapeutic genes including human factor IX. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4973913 Tue, 31 May 2011 23:00:00 +0100 4973913 The multisubstrate deoxyribonucleoside kinase of D. melanogaster as a therapeutic suicide gene of breast cancer cells http://www.medworm.com/index.php?rid=4930490&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1573 ConclusionsThese results suggest that the Dm‐dNK/nucleoside analog system could be a novel therapeutic strategy to treat breast cancer and improve anti‐tumor efficacy, as well as optimize approaches for suicide gene therapy. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4930490 Sat, 30 Apr 2011 23:00:00 +0100 4930490 Conference Calendar http://www.medworm.com/index.php?rid=4909387&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1572 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4909387 Sat, 30 Apr 2011 23:00:00 +0100 4909387 Development of a successive targeting liposome with multi‐ligand for efficient targeting gene delivery http://www.medworm.com/index.php?rid=4909386&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1569 ConclusionsSTL with multi‐ligand was able to overcome the various barriers to target nucleus and special cells and present distinctive transgenic expression. Therefore, it has a great potential for gene therapy as a nonviral carrier. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4909386 Sat, 30 Apr 2011 23:00:00 +0100 4909386 In this issue http://www.medworm.com/index.php?rid=4909385&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1571 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4909385 Sat, 30 Apr 2011 23:00:00 +0100 4909385 Issue Information http://www.medworm.com/index.php?rid=4909384&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1570 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4909384 Sat, 30 Apr 2011 23:00:00 +0100 4909384 Development of a successive targeting liposome (STL) with multi‐ligand for efficient targeting gene delivery http://www.medworm.com/index.php?rid=4826977&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1569 ConclusionsSTL with multi‐ligand was able to overcome the various barriers to target nucleus and special cells and present distinctive transgenic expression. Therefore it has a great potential for gene therapy as a non‐viral carrier. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4826977 Thu, 31 Mar 2011 23:00:00 +0100 4826977 Adeno‐associated virus‐mediated human acidic fibroblast growth factor expression promotes functional recovery of spinal cord–contused rats http://www.medworm.com/index.php?rid=4797314&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1568 ConclusionsThese data suggest that supplement of aFGF improve the functional recovery of spinal cord–contused rats and that AAV‐aFGF‐mediated gene transfer could be a clinically feasible therapeutic approach for patients after nervous system injuries. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4797314 Thu, 31 Mar 2011 23:00:00 +0100 4797314 An Evaluation of Site‐Specific Immune Responses Directed Against First‐Generation Adenoviral Vectors Administered by Convection‐Enhanced Delivery http://www.medworm.com/index.php?rid=4787149&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1567 ConclusionThese results indicate that CED is capable of mediating widespread adenoviral vector distribution, although these vectors are associated with significant tissue toxicity that may render their safe application in clinical trials unfeasible. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4787149 Thu, 31 Mar 2011 23:00:00 +0100 4787149 Conference Calendar http://www.medworm.com/index.php?rid=4752125&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1564 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752125 Thu, 31 Mar 2011 23:00:00 +0100 4752125 Evaluation of nonbiomedical and biomedical grade alginates for the transplantation of genetically modified articular chondrocytes to cartilage defects in a large animal model in vivo http://www.medworm.com/index.php?rid=4752124&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1557 ConclusionsThis approach may be of value to study the effects of potential therapeutic genes upon cartilage repair in a clinically relevant setting. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752124 Thu, 31 Mar 2011 23:00:00 +0100 4752124 Attenuated Actinobacillus pleuropneumoniae as a bacterial vector for expression of Mycoplasma hyopneumoniae P36 gene http://www.medworm.com/index.php?rid=4752123&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1556 ConclusionsThe mutant strain SLW36 can induce antibody against p36 and M. hyopneumoniae. The mutant strain SLW36 has the potential to be used as a live vaccine for protection against A. pleuropneumoniae and M. hyopneumoniae. Studies in pigs are needed to confirm protective levels of antibodies and to check for rare side‐effects of the vaccine. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752123 Thu, 31 Mar 2011 23:00:00 +0100 4752123 Sustained inhibition of IL‐6 and IL‐8 expression by decoy ODN to NF‐κB delivered through respirable large porous particles in LPS‐stimulated cystic fibrosis bronchial cells http://www.medworm.com/index.php?rid=4752122&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1546 ConclusionsOur observations indicate that respirable biodegradable dec‐ODN LPP may represent a promising strategy for inhibiting NF‐κB transcriptional activity and related gene expression and, thus, reduce lung chronic inflammation in CF patients. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752122 Thu, 31 Mar 2011 23:00:00 +0100 4752122 In this issue http://www.medworm.com/index.php?rid=4752121&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1562 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752121 Thu, 31 Mar 2011 23:00:00 +0100 4752121 Issue Information http://www.medworm.com/index.php?rid=4752120&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1560 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752120 Thu, 31 Mar 2011 23:00:00 +0100 4752120 Minimum requirement of donor cells to reduce the glycolipid storage following bone marrow transplantation in a murine model of Fabry disease http://www.medworm.com/index.php?rid=4752119&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1566 Conclusions:In FD mice, reconstitution with 100% donor cells is not required to obtain a therapeutic effect following bone marrow transplantation. These results suggest that a 30% gene correction might be sufficient to reverse the disease manifestations in FD. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4752119 Thu, 31 Mar 2011 23:00:00 +0100 4752119 Inhibition of highly pathogenic avian H5N1 influenza virus propagation by RNA oligonucleotides targeting the PB2 gene in combination with celecoxib http://www.medworm.com/index.php?rid=4657320&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1558 In this study, we attempted to explore if the combinational use of a viral gene‐targeted agent and immunomodulator is feasible as a new strategy against the H5N1 infection. Four antisense RNA oligonucleotides targeting the polymerase basic protein 2 (PB2) gene of H5N1‐HPIV were designed and screened for their ability to inhibit H5N1 influenza viral propagation. In MDCK cells, the RNA oligonucleotides efficiently inhibited viral replication, as measured by hemagglutinin production, plaque formation and viral RNA expression assays. In a mouse infection model, a combinational treatment in mice with the PB2 oligonucleotides and celecoxib significantly reduced the viral load, regulated cytokine profiles, and improved lung lesions and animal survival in comparison with the single use of eith... The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4657320 Tue, 29 Mar 2011 23:00:00 +0100 4657320 Knock down of GAD67 protein levels normalizes neuronal activity in a rat model of Parkinson's disease http://www.medworm.com/index.php?rid=4657322&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1555 ConclusionsWe conclude that RNAi directed against GAD67 may be a valid approach to correct the dysregulation of the basal ganglia circuit in a rat model of Parkinson's disease. The possibility to correct for a loss of dopamine using non‐dopamimetic tools is interesting as it may be more directed towards the casual mechanisms of the motor symptoms. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4657322 Mon, 28 Mar 2011 23:00:00 +0100 4657322 Evaluation of non‐biomedical and biomedical grade alginates for the transplantation of genetically modified articular chondrocytes to cartilage defects in a large animal model in vivo http://www.medworm.com/index.php?rid=4657321&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1557 ConclusionsThis approach may be of value to study the effects of potential therapeutic genes upon cartilage repair in a clinically relevant setting. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4657321 Mon, 28 Mar 2011 23:00:00 +0100 4657321 Endostatin‐ and interleukin‐2‐expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma http://www.medworm.com/index.php?rid=4511019&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1547 ConclusionRetroviral bicistronic gene transfer led to the secretion of functional ES and IL‐2 that were sufficiently active to inhibit tumor angiogenesis and tumor cell proliferation and to increase the infiltration of immune cells. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4511019 Tue, 22 Feb 2011 00:00:00 +0100 4511019 Intracellular efficacy of tumor‐targeting group I intron‐based trans‐splicing ribozyme http://www.medworm.com/index.php?rid=4476339&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1545 Conclusion:We analyzed ribozyme efficacy and determined the most influential factors of its trans‐splicing reaction in mammalian cell lines and in vivo. This study could provide insights into the optimization of the trans‐splicing ribozyme‐based RNA replacement approach to cancer treatment. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4476339 Tue, 15 Feb 2011 00:00:00 +0100 4476339 Stem cell antigen‐1 positive cell–based systemic human growth hormone gene transfer strategy increased endosteal bone resorption and bone loss in mice http://www.medworm.com/index.php?rid=4476342&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1542 ConclusionTargeted expression of hGH in cells of marrow cavity through the Sca‐1+ cell‐based gene transfer strategy increased circulating IGF‐I and decreased endosteal bone mass through an increase in resorption in recipient mice. These results indicate that high local levels of hGH or IGF‐I in the bone marrow microenvironment enhanced resorption, which are consistent with previous findings in transgenic mice with targeted bone IGF‐I expression that high local IGF‐I expression increased bone remodeling that favored a net bone loss. Thus, the GH and/or IGF‐I would not be an appropriate transgene for use in this Sca‐1+ cell‐based gene transfer strategy to promote endosteal bone formation. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4476342 Mon, 14 Feb 2011 00:00:00 +0100 4476342 Complete restoration of phenylalanine oxidation in phenylketonuria mouse by a self‐complementary adeno‐associated virus vector http://www.medworm.com/index.php?rid=4476341&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1543 ConclusionComplete phenotypic correction of PKU mice was achieved by the scAAV8 vector for the longest duration reported to date. The vector overcame the female‐specific disadvantage in AAV‐mediated liver transduction, thus it offers a promising platform of long‐lasting gene therapy for PKU. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4476341 Mon, 14 Feb 2011 00:00:00 +0100 4476341 Infant lungs are preferentially infected by adenovirus and herpes simplex virus type 1 vectors: role of the tissue mesenchymal cells http://www.medworm.com/index.php?rid=4476340&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1544 Conclusion:the similarity of the human and mouse tissues, with respect to viral vector tropism, validates the mouse model in studies of gene transfer to the lung. Furthermore, the results should facilitate improved design of gene therapy trials for lung related diseases in young and adults patients. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4476340 Mon, 14 Feb 2011 00:00:00 +0100 4476340 Switching the fiber knob of oncolytic adenoviruses to avoid neutralizing antibodies in human cancer patients http://www.medworm.com/index.php?rid=4746947&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1565 Conclusions:Our results extend previous preclinical reports into human cancer patients and suggest that modification of fiber knob is a feasible strategy to circumvent NAb response in patients receiving multiple rounds of oncolytic adenoviruses. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4746947 Tue, 01 Feb 2011 00:00:00 +0100 4746947 Mesenchymal stem cells as vehicles for targeted delivery of antiangiogenic protein to solid tumors http://www.medworm.com/index.php?rid=4657319&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1552 ConclusionhMSC may serve as an effective platform for the targeted delivery of therapeutic proteins to cancer sites. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4657319 Tue, 01 Feb 2011 00:00:00 +0100 4657319 Attenuated Actinobcillus pleuropneumoniae as a bacterial vector for expression of Mycoplasma hyopneumoniae P36 gene http://www.medworm.com/index.php?rid=4625684&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1556 ConclusionThe mutant strain SLW36 can induce antibody against p36 and M. hyopneumoniae. The mutant strain SLW36 has the potential to be used as a live vaccine for protection against A. pleuropneumoniae and M. hyopneumoniae. Studies in pigs are needed to confirm protective levels of antibodies and to check for rare side effects of the vaccine. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4625684 Tue, 01 Feb 2011 00:00:00 +0100 4625684 Characterization of factors that determine lentiviral vector tropism in skin tissue using an ex vivo model http://www.medworm.com/index.php?rid=4609580&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1554 ConclusionsTaken together the results indicated that lentiviral vector tropism in the three dimensional skin tissue is distinct of the tropism to keratinocytes in culture and is dependent on a complex interplay of extracellular restrictions. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4609580 Tue, 01 Feb 2011 00:00:00 +0100 4609580 Conference Calendar http://www.medworm.com/index.php?rid=4589146&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1551 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4589146 Tue, 01 Feb 2011 00:00:00 +0100 4589146 Visualization of intracellular trafficking of Math1 protein in different cell types with a newly‐constructed nonviral gene delivery plasmid http://www.medworm.com/index.php?rid=4589145&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1537 ConclusionsThe newly‐constructed plasmid pcDNA6.2/C‐EmGFP‐Math1 was suitable for nonviral gene delivery of Math1. Unique intracellular trafficking of Math1 with dynamics from the cytoplasm to the nucleus was demonstrated. The modification of mesenchymal stem cells by Math1 gene delivery and by brain‐derived neurotrophic factor and glial cell line‐derived neurotrophic factor treatments can potentially be applied to cell replacement for the treatment of cochlear spiral ganglion cell loss in deafness. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4589145 Tue, 01 Feb 2011 00:00:00 +0100 4589145 Stem cell antigen‐1 positive cell‐based systemic human growth hormone gene transfer strategy increases endosteal bone resorption and bone loss in mice http://www.medworm.com/index.php?rid=4589144&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1542 ConclusionsTargeted expression of hGH in cells of marrow cavity through the Sca‐1+ cell‐based gene transfer strategy increased circulating IGF‐I and decreased endosteal bone mass through an increase in resorption in recipient mice. These results indicate that high local levels of hGH or IGF‐I in the bone marrow microenvironment enhanced resorption, which is consistent with previous findings in transgenic mice with targeted bone IGF‐I expression showing that high local IGF‐I expression increased bone remodeling, favoring a net bone loss. Thus, GH and/or IGF‐I would not be an appropriate transgene for use in this Sca‐1+ cell‐based gene transfer strategy to promote endosteal bone formation. Published 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4589144 Tue, 01 Feb 2011 00:00:00 +0100 4589144 In this issue http://www.medworm.com/index.php?rid=4589143&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1550 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4589143 Tue, 01 Feb 2011 00:00:00 +0100 4589143 Issue Information http://www.medworm.com/index.php?rid=4589142&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1549 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4589142 Tue, 01 Feb 2011 00:00:00 +0100 4589142 A lentiviral vector pseudotype suitable for vaccine development http://www.medworm.com/index.php?rid=4569420&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1553 ConclusionRRV envelope is a suitable candidate to be used for construction of an LV producer cell line. LV vaccines with RRV envelope can be tested in mice and in human immune cell cultures. The higher dose of RRV‐LV required for vaccine efficacy compared to VSV‐G‐LV will partly be offset by ease of production. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4569420 Sat, 01 Jan 2011 00:00:00 +0100 4569420 Nuclear targeted delivery of macromolecules to retina and cornea http://www.medworm.com/index.php?rid=4511018&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1548 ConclusionsOur results demonstrate that NBP can deliver small and large molecules into retinal and corneal cells and plasmid DNA into retinal cells and hence may be useful for the delivery of therapeutics to the eye. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4511018 Sat, 01 Jan 2011 00:00:00 +0100 4511018 Sustained inhibition of IL‐6 and IL‐8 expression by ODN decoy to NF‐κB delivered through respirable large porous particles in LPS‐stimulated cystic fibrosis bronchial cells http://www.medworm.com/index.php?rid=4476338&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1546 ConclusionsOur observations indicate that respirable biodegradable dec‐ODN LPP may represent a promising strategy to inhibit NF‐κB transcriptional activity and related gene expression and, thus, reduce the lung chronic inflammation in CF patients. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4476338 Sat, 01 Jan 2011 00:00:00 +0100 4476338 Conference Calendar http://www.medworm.com/index.php?rid=4398184&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1539 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4398184 Sat, 01 Jan 2011 00:00:00 +0100 4398184 Prophylactic, therapeutic and immune enhancement effect of liposome‐encapsulated PolyICLC on highly pathogenic H5N1 influenza infection http://www.medworm.com/index.php?rid=4398183&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1536 ConclusionsTaken together, the data obtained in the present study suggest that strong consideration should be given for the use of LE‐PolyICLC as prophylactic and therapeutic agents and also as a vaccination adjuvant to combat highly pathogenic influenza infection and its associated complications such as pulmonary fibrosis. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4398183 Sat, 01 Jan 2011 00:00:00 +0100 4398183 Transplantation of CTLA4Ig gene‐transduced adipose tissue‐derived mesenchymal stem cells reduces inflammatory immune response and improves Th1/Th2 balance in experimental autoimmune thyroiditis http://www.medworm.com/index.php?rid=4398182&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1531 ConclusionAlthough CTLA4Ig‐MSC transplantation had better result in reduction of serum TgAA, both of ATMSC and CTLA4Ig‐MSC transplantations are promising treatments for autoimmune thyroiditis judging from the results of histopathology and cytokine analysis. They may be attractive candidates for treating organ‐specific autoimmune disease. Copyright © 2011 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4398182 Sat, 01 Jan 2011 00:00:00 +0100 4398182 In this issue http://www.medworm.com/index.php?rid=4398181&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1541 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4398181 Sat, 01 Jan 2011 00:00:00 +0100 4398181 Editorial Board http://www.medworm.com/index.php?rid=4398180&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1540 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4398180 Sat, 01 Jan 2011 00:00:00 +0100 4398180 Anti‐DNA antibody modified coronary stent for plasmid gene delivery: results obtained from a porcine coronary stent model http://www.medworm.com/index.php?rid=4270702&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1529 ConclusionsFor the first time, we report the successful use of anti‐DNA antibody‐immobilized stent as plasmid gene delivery system that possess high efficiency and site‐specificity in a porcine coronary stent model. The novel system showed long‐term therapeutic effects on the inhibition of restenosis when pcDNA3.1‐iNOS was tethered on the stent. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4270702 Fri, 17 Dec 2010 00:00:00 +0100 4270702 Creation of a panel of vectors based on ape adenovirus isolates http://www.medworm.com/index.php?rid=4270701&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1530 ConclusionsE1‐deleted adenovirus vectors can be created from a wide variety of ape adenoviruses that can be rescued and propagated in HEK 293 cells. The prevalence of pre‐existing antibodies that can neutralize these adenoviruses in human populations is low. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4270701 Fri, 17 Dec 2010 00:00:00 +0100 4270701 Antiglioma effects of combined use of a baculovirual vector expressing wild‐type p53 and sodium butyrate http://www.medworm.com/index.php?rid=4240696&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1522 ConclusionsThese findings suggest a new approach to treat glioblastoma using baculovirus‐mediated gene transfer in combination with administration of histone deacetylase inhibitor. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4240696 Tue, 07 Dec 2010 00:00:00 +0100 4240696 Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell http://www.medworm.com/index.php?rid=4240695&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1526 ConclusionsImproved transfection efficiency of PEI2LA was attributed to enhanced association with the nucleus, which may be a result of hydrophobic interaction between the lipid moieties on the modified lipopolymer and the nuclear membrane. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4240695 Tue, 07 Dec 2010 00:00:00 +0100 4240695 Conference Calendar http://www.medworm.com/index.php?rid=4257233&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1533 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257233 Wed, 01 Dec 2010 00:00:00 +0100 4257233 The 15th Annual Meeting 2009 Japan Society of Gene Therapy http://www.medworm.com/index.php?rid=4257232&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1532 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257232 Wed, 01 Dec 2010 00:00:00 +0100 4257232 Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein‐1 delivery in mice http://www.medworm.com/index.php?rid=4257231&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1528 ConclusionsCollectively, these results demonstrate that an adequate amount of CCL2/MCP‐1, together with the HSV‐tk/GCV system, may induce T helper 1‐polarized antitumor effects without inducing tumor angiogenesis in the microenvironment of intrahepatic HCC progression. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257231 Wed, 01 Dec 2010 00:00:00 +0100 4257231 Controlled nonviral gene delivery and expression using stable neural stem cell line transfected with a hypoxia‐inducible gene expression system http://www.medworm.com/index.php?rid=4257230&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1527 ConclusionsWe propose that NSCs engineered with the Epo‐SV‐therapeutic gene will be valuable for developing a controllable stem cell‐mediated nonviral gene therapy for SCI or other central nervous system diseases accompanied with chronic or episodic hypoxic/ischemic stresses. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257230 Wed, 01 Dec 2010 00:00:00 +0100 4257230 Electroporation to deliver plasmid DNA into rat dental tissues http://www.medworm.com/index.php?rid=4257229&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1521 ConclusionsThe present study demonstrates for the first time the feasibility of electroporation to locally deliver plasmids into dental tissues. Parameters affecting electroporation to deliver plasmids into the dental tissues were optimized. This protocol could be used to deliver short hairpin RNA or genes of interest into the dental tissues to regulate tooth eruption. Thus, it may be possible to develop nonsurgical treatments for inducing the eruption of impacted teeth. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257229 Wed, 01 Dec 2010 00:00:00 +0100 4257229 Publisher's Note http://www.medworm.com/index.php?rid=4257228&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1535 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257228 Wed, 01 Dec 2010 00:00:00 +0100 4257228 In this issue http://www.medworm.com/index.php?rid=4257227&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1534 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4257227 Wed, 01 Dec 2010 00:00:00 +0100 4257227 Antibody‐directed lentiviral gene transduction in early immature hematopoietic progenitor cells http://www.medworm.com/index.php?rid=4195406&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1518 ConclusionsGene transfer to lineage− early immature hematopoietic progenitors from human umbilical CB was obtained using CD133, ABCG2 or HLA‐1 antibodies conjugated to lentiviruses pseudotyped with modified Sindbis viral Env proteins. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4195406 Mon, 22 Nov 2010 00:00:00 +0100 4195406 Novel tumour‐specific promoters for transcriptional targeting of hepatocellular carcinoma by herpes simplex virus vectors http://www.medworm.com/index.php?rid=4195405&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1519 ConclusionsWe identified and experimentally characterized novel hepatoma‐specific promoters, which were valuable for cancer‐specific gene therapy, using HSV‐1 vectors. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4195405 Mon, 22 Nov 2010 00:00:00 +0100 4195405 Amphiphilic, low molecular weight poly(ethylene imine) derivatives with enhanced stability for efficient pulmonary gene delivery http://www.medworm.com/index.php?rid=4252322&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1538 ConclusionsA new set of polymers with low toxicity and high stability was synthesized, which contains promising candidates for pulmonary gene transfer, as documented by in vivo experiments in mice. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4252322 Mon, 01 Nov 2010 00:00:00 +0100 4252322 Visualization of intracellular trafficking of Math1 protein in different cell types with a newly constructed non‐viral gene delivery plasmid http://www.medworm.com/index.php?rid=4248064&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1537 ConclusionsThe newly constructed plasmid pcDNA6.2/C‐EmGFP‐Math1 was suitable for non‐viral gene delivery of Math1. Unique intracellular trafficking of Math1 with dynamics from the cytoplasm to the nucleus was demonstrated. The modification of MSCs by Math1 gene delivery and by BDNF and GDNF treatments can potentially be applied to cell replacement for the treatment of cochlear spiral ganglion cell loss in deafness. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4248064 Mon, 01 Nov 2010 00:00:00 +0100 4248064 Prophylactic, therapeutic and immune enhancement effect of LE‐PolyICLC on highly pathogenic H5N1 influenza infection http://www.medworm.com/index.php?rid=4240694&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1536 ConclusionsTaken together, the data suggest that strong consideration should be given for the use of LE‐PolyICLC as prophylactic and therapeutic agents and also as a vaccination adjuvant to combat highly pathogenic influenza infection and its associated complications such as pulmonary fibrosis. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4240694 Mon, 01 Nov 2010 00:00:00 +0100 4240694 Transplantation of CTLA4Ig gene‐transduced adipose tissue‐derived mesenchymal stem cells reduce inflammatory immune response and improve Th1/Th2 balance in experimental autoimmune thyroiditis http://www.medworm.com/index.php?rid=4202257&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1531 Conclusion:Although CTLA4Ig‐MSC transplantation had better result in reduction of serum TgAA, both of ATMSC and CTLA4Ig‐MSC transplantation is a promising treatment for autoimmune thyroiditis judging from the results of histopathology and cytokine analysis. They may be an attractive candidate for treating organ‐specific autoimmune disease. Copyright © 2010 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4202257 Mon, 01 Nov 2010 00:00:00 +0100 4202257 Conference Calendar http://www.medworm.com/index.php?rid=4195411&cid=s_33648_50_f&fid=33648&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fjgm.1524 (Source: The Journal of Gene Medicine) The Journal of Gene Medicine journals http://www.medworm.com/rss/comments.php?id=4195411 Mon, 01 Nov 2010 00:00:00 +0100 4195411