MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'The Korean Journal of Laboratory Medicine' source. Tue, 29 Nov 2011 19:36:40 +0100 http://www.medworm.com/index.php?rid=5345252&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016674%26dopt%3DAbstract CONCLUSIONS: Patients with MPE have aggressive clinical and laboratory characteristics. The preponderance of IgD myeloma in MPE patients is a noteworthy finding because IgD myeloma is a rare subtype. Elevated ADA activity in the pleural fluid is also noteworthy, and may be helpful for detecting MPE. Physicians treating myeloma patients should monitor the development of MPE and consider the possibility of a worse clinical course. PMID: 22016674 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345252 Sat, 01 Oct 2011 04:00:00 +0100 5345252 http://www.medworm.com/index.php?rid=5345251&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016675%26dopt%3DAbstract CONCLUSIONS: This study provides comparison data for Tregs in AML, MDS, and autoimmune hematologic diseases, and would be helpful for understanding the different immunologic bases of various hematologic diseases. Treg measurement using CD4, CD25, and/or FoxP3 in PB rather than in BM seems to be practical for routine hematologic purposes. Large-scale analysis of the diagnostic role of Treg measurement is needed. PMID: 22016675 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345251 Sat, 01 Oct 2011 04:00:00 +0100 5345251 http://www.medworm.com/index.php?rid=5345250&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016676%26dopt%3DAbstract CONCLUSIONS: CE is comparable to CA for reliable measurement of Hb fractions. It is suitable for screening of hemoglobinopathies in many clinical laboratories. PMID: 22016676 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345250 Sat, 01 Oct 2011 04:00:00 +0100 5345250 http://www.medworm.com/index.php?rid=5345249&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016677%26dopt%3DAbstract CONCLUSIONS: Values of LH measured from a single blood sample obtained at 45 min in the GnRH stimulation test may be adequate for the diagnosis of CPP. Two samples, taken at 30 and 45 min after stimulation, were able to accurately diagnose CPP in 100% of the patients in this study. PMID: 22016677 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345249 Sat, 01 Oct 2011 04:00:00 +0100 5345249 http://www.medworm.com/index.php?rid=5345248&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016678%26dopt%3DAbstract CONCLUSIONS: The performance of our revised techniques for MS/MS detection and enzyme assays was of the generally acceptable standard. To our knowledge, this is the first report on the use of MS/MS for newborn screening of LSDs in an Asian population. PMID: 22016678 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345248 Sat, 01 Oct 2011 04:00:00 +0100 5345248 http://www.medworm.com/index.php?rid=5345247&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016679%26dopt%3DAbstract CONCLUSIONS: The qnr genes were highly prevalent in Enterobacteriaceae, primarily the qnrB subtypes. They were closely associated with EBSL and AmpC beta-lactamase. PMID: 22016679 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345247 Sat, 01 Oct 2011 04:00:00 +0100 5345247 http://www.medworm.com/index.php?rid=5345246&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016680%26dopt%3DAbstract CONCLUSIONS: Several differences in carbapenem, aminoglycoside, and fluoroquinolone resistance gene content were detected among Acb complex isolates. However, most Acb complex isolates (87.5%) possessed integrons, carbapenemases, AMEs, and mutations in gyrA. The co-occurrence of several resistance determinants may present a significant threat. PMID: 22016680 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345246 Sat, 01 Oct 2011 04:00:00 +0100 5345246 http://www.medworm.com/index.php?rid=5345245&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016681%26dopt%3DAbstract CONCLUSIONS: In patients receiving immunosuppressive agents, both IGRA conversions and reversions were observed. Serial IGRA testing may not be needed in patients with a positive initial IGRA result showing high IFN-γ levels, because of high consistency in the test results. PMID: 22016681 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345245 Sat, 01 Oct 2011 04:00:00 +0100 5345245 http://www.medworm.com/index.php?rid=5345244&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016682%26dopt%3DAbstract In this study, we determined the aminoglycoside susceptibility profiles of 58 AAC(6')-Ib-producing Enterobacter cloacae isolates. On the basis of the CLSI and EUCAST breakpoints, a large proportion (84.5% and 55.2%, respectively) of these 58 isolates were found to be susceptible to amikacin. However, among the isolates that were shown to be anikacin-susceptible according to the CLSI and EUCAST breakpoints, only 30.6% and 18.8% isolates, respectively, could be considered to have intermediate resistance on the basis of the EUCAST expert rules. Further studies should be conducted to determine the aminoglycoside susceptibility profiles of aac(6')-Ib-harboring isolates from various geographic regions and to monitor the therapeutic efficacy of amikacin in infections caused by these isolates. ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345244 Sat, 01 Oct 2011 04:00:00 +0100 5345244 http://www.medworm.com/index.php?rid=5345243&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016683%26dopt%3DAbstract Authors: Jang MH, Choi GE, Shin BM, Lee SH, Kim SR, Chang CL, Kim JM Abstract Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularl... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345243 Sat, 01 Oct 2011 04:00:00 +0100 5345243 http://www.medworm.com/index.php?rid=5345242&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016684%26dopt%3DAbstract We report a rare case of multiple myeloma with biclonal gammopathy (IgG kappa and IgA lambda type) in a 58-year-old man with prostate cancer who presented with lower back pain. Through computed tomography (CT) imaging, an osteolytic lesion at the L3 vertebra and an enhancing lesion of the prostate gland with multiple lymphadenopathies were found. In the whole body positron emission tomography-computed tomography (PET-CT), an additional osteoblastic bone lesion was found in the left ischial bone. A prostate biopsy was performed, and adenocarcinoma was confirmed. Decompression surgery of the L3 vertebra was conducted, and the pathologic result indicated that the lesion was a plasma cell neoplasm. Immunofixation electrophoresis showed the presence of biclonal gammopathy (IgG kappa and IgA lam... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345242 Sat, 01 Oct 2011 04:00:00 +0100 5345242 http://www.medworm.com/index.php?rid=5345241&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016685%26dopt%3DAbstract We report a novel mutation of the F11 gene in an 18-year-old asymptomatic Korean woman with mild FXI deficiency. Pre-operative laboratory screen tests for lipoma on her back revealed slightly prolonged activated partial thromboplastin time (45.2 sec; reference range, 23.2-39.4 sec). Her FXI activity (35%) was slightly lower than the normal FXI activity (reference range, 50-150%). Direct sequence analysis of the F11 gene revealed a heterozygous A to G substitution in nucleotide 1517 (c.1517A>G) of exon 13, resulting in the substitution of aspartic acid with glycine in codon 506 (p.Asp506Gly). To the best of our knowledge, the Asp506Gly is a novel missense mutation, and this is the first genetically confirmed case of mild FXI deficiency in Korea. PMID: 22016685 [PubMed - in process] (... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345241 Sat, 01 Oct 2011 04:00:00 +0100 5345241 http://www.medworm.com/index.php?rid=5345240&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016686%26dopt%3DAbstract Authors: Ko DH, Chang HE, Song SH, Yoon H, Park KU, Song J Abstract Alpha 1-antitrypsin (AAT) deficiency is a genetic disorder that primarily affects the lungs and liver. While AAT deficiency is one of the most common genetic disorders in the Caucasian population, it is extremely rare in Asians. Here, we report the case of a 36-year-old Korean woman with AAT deficiency who visited the emergency department of our hospital for the treatment of progressive dyspnea that had begun 10 years ago. She had never smoked. Chest computed tomography revealed panlobular emphysema in both lungs, which suggested AAT deficiency. The serum AAT level was 33 mg/dL (reference interval: 90-200 mg/dL). Four exons of the SERPINA1 gene, which is responsible for AAT deficiency, and their flanking regions we... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345240 Sat, 01 Oct 2011 04:00:00 +0100 5345240 http://www.medworm.com/index.php?rid=5345239&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016687%26dopt%3DAbstract We report the case of a patient with a urinary tract infection caused by KPC-2-producing K. pneumoniae. This is the second report of a KPC-2-producing K. pneumoniae infection in Korea, but the multilocus sequence type was ST258. The KPC-2-producing isolate was resistant to all tested β-lactams (including imipenem and meropenem), amikacin, tobramycin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole, but was susceptible to gentamicin, colistin, polymyxin B, and tigecycline. The KPC-2-producing isolate was negative to phenotypic extended-spectrum β-lactamase (ESBL) and AmpC detection tests and positive to modified Hodge test and carbapenemase inhibition test with aminophenylboronic acid. PMID: 22016687 [PubMed - in process] (Source: The Korean Journal of Laboratory Medici... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345239 Sat, 01 Oct 2011 04:00:00 +0100 5345239 http://www.medworm.com/index.php?rid=5345238&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016688%26dopt%3DAbstract Authors: Park H, Hyun J, Park SS, Park MH, Song EY Abstract Loss of heterozygosity (LOH) in chromosome 6p has been reported in a number of tumors and some hematologic malignancies, including ALL. LOH in chromosome 6p, on which the HLA genes are located, can give rise to false homozygosity results in HLA genotyping of patients with hematologic malignancies. Here we report false homozygosity results in HLA genotyping due to the loss of whole chromosome 6 in the neoplastic cells of a patient with ALL. A 33-yr-old Korean female patient was admitted for the evaluation of leukocytosis detected during a workup for headache. Her initial white blood cell count was 336.9×10(9)/L with 84% of blasts in the differential count. Precursor-B lymphoblastic leukemia was diagnosed from a subsequent ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345238 Sat, 01 Oct 2011 04:00:00 +0100 5345238 http://www.medworm.com/index.php?rid=5345237&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22016689%26dopt%3DAbstract Authors: Lee YJ PMID: 22016689 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5345237 Sat, 01 Oct 2011 04:00:00 +0100 5345237 http://www.medworm.com/index.php?rid=5057209&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779183%26dopt%3DAbstract Authors: Jo Y, Kim SH, Koh K, Park J, Shim YB, Lim J, Kim Y, Park YJ, Han K Hematology analyzers may ineffectively recognize abnormal cells, and manual differential counts may be imprecise for leukopenic samples. We evaluated the efficacy of the Hematoflow method for determining the leukocyte differential in leukopenic samples and compared this method with the manual differential method. PMID: 21779183 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057209 Thu, 30 Jun 2011 23:00:00 +0100 5057209 http://www.medworm.com/index.php?rid=5057208&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779184%26dopt%3DAbstract Authors: Pornprasert S, Wiengkum T, Srithep S, Chainoi I, Singboottra P, Wongwiwatthananukit S Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. PMID: 21779184 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057208 Thu, 30 Jun 2011 23:00:00 +0100 5057208 http://www.medworm.com/index.php?rid=5057207&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779185%26dopt%3DAbstract Authors: Park KJ, Kwon EH, Kim HJ, Kim SH Fibrin-related markers (FRM) such as fibrin monomer (FM) and D-dimer (DD) are considered useful biological markers for the diagnosis of disseminated intravascular coagulation (DIC). However, no studies on the diagnostic performance of different FRMs have been published in Korea. The aim of this study was to evaluate the diagnostic performance of FM for DIC in comparison with DD. PMID: 21779185 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057207 Thu, 30 Jun 2011 23:00:00 +0100 5057207 http://www.medworm.com/index.php?rid=5057206&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779186%26dopt%3DAbstract Authors: Im M, Chae H, Kim T, Park HH, Lim J, Oh EJ, Kim Y, Park YJ, Han K Since the recent introduction of radioimmunotherapy (RIT) using antibodies against cluster of differentiation (CD) 45 for the treatment of lymphoma, the clinical significance of the CD45 antigen has been increasing steadily. Here, we analyzed CD45 expression on lymphocyte subsets using flow cytometry in order to predict the susceptibility of normal lymphocytes to RIT. PMID: 21779186 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057206 Thu, 30 Jun 2011 23:00:00 +0100 5057206 http://www.medworm.com/index.php?rid=5057205&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779187%26dopt%3DAbstract Authors: Ma Y, Kwon EH, Lee JE, Kim K, Kim HJ, Kim SH Amyloidosis is a heterogeneous group of diseases in which misfolding of extracellular proteins is the pathogenic factor. Light chain amyloidosis (AL) is the most common form of amyloidosis, and the causative proteins in AL are the immunoglobulin light chains produced by clonal plasma cells. Hemorrhagic events, ranging from mild subcutaneous hemorrhage to life-threatening bleeding, account for a significant proportion of morbidities and mortality in AL patients. Deficiency of factor X from deposition into amyloid fibrils has been reported to be the most common acquired factor deficiency in AL. We herein report 2 patients with acquired factor X deficiency in AL. A 55-yr-old woman with AL had a prolonged prothrombin time (PT) and an ac... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057205 Thu, 30 Jun 2011 23:00:00 +0100 5057205 http://www.medworm.com/index.php?rid=5057204&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779188%26dopt%3DAbstract In this study, we present the case of an 80-yr-old man who was admitted to the hospital because of a 1-month history of lower back pain and dyspnea. He was diagnosed with MAHA on the basis of the laboratory findings that revealed anemia with schistocytes, decreased haptoglobin levels, and a negative direct Coombs' test. Bone marrow examination, which was performed because of the progression of anemia, revealed bone marrow metastases of signet ring cell carcinoma with extensive bone marrow necrosis. However, the primary origin of this signet ring cell carcinoma was not found. When the cause of progressive MAHA is unknown, the possibility of cancer-associated MAHA must be excluded by performing additional tumor workup, including the detection of tumor markers, gastric and colorectal endoscop... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057204 Thu, 30 Jun 2011 23:00:00 +0100 5057204 http://www.medworm.com/index.php?rid=5057203&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779189%26dopt%3DAbstract Authors: Kotani K, Sakane N Circulating leptin:adiponectin ratio (L:A) is a potential surrogate marker for cardiometabolic diseases; however, the relationship of the L:A with the occurrence of metabolic syndrome (MetS) has not yet been fully explored in the general Japanese population. PMID: 21779189 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057203 Thu, 30 Jun 2011 23:00:00 +0100 5057203 http://www.medworm.com/index.php?rid=5057202&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779190%26dopt%3DAbstract Authors: Chen H, Zhang L, Bi X, Deng X Measurement uncertainty characterizes the dispersion of the quantity values attributed to a measurand. Although this concept was introduced to medical laboratories some years ago, not all medical researchers are familiar with it. Therefore, the evaluation and expression of measurement uncertainty must be highlighted using a practical example. PMID: 21779190 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057202 Thu, 30 Jun 2011 23:00:00 +0100 5057202 http://www.medworm.com/index.php?rid=5057200&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779191%26dopt%3DAbstract Authors: Khan DA, Sharif MS, Khan FA Early diagnosis is the cornerstone of management of acute myocardial infarction (AMI). We aimed to compare the diagnostic accuracy of high-sensitivity troponin T (hs-cTnT) with myeloperoxidase (MPO) and pregnancy-associated plasma protein A (PAPP-A) for early diagnosis of AMI in patients at the time of presentation to the emergency department (ED). PMID: 21779191 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057200 Thu, 30 Jun 2011 23:00:00 +0100 5057200 http://www.medworm.com/index.php?rid=5057198&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779192%26dopt%3DAbstract Authors: Kim JS, Lim CS, Kim YK, Lee KN, Lee CK Human bocavirus (HBoV) is a newly identified viral pathogen, and its clinical epidemiology and significance in respiratory infections have not yet been completely elucidated. We investigated the prevalence of HBoV infection and the association between viral (HBoV) load and clinical features of the infection in patients of all age-groups. PMID: 21779192 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057198 Thu, 30 Jun 2011 23:00:00 +0100 5057198 http://www.medworm.com/index.php?rid=5057197&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779193%26dopt%3DAbstract Authors: Kim SJ, Uh Y, Jang IH, Lee KS, Park SD, Yoon KJ In order to determine the clinical usefulness of the MicroScan (Siemens Healthcare Diagnostics, USA) MICroSTREP plus antimicrobial panel (MICroSTREP) for testing antimicrobial susceptibility of β-hemolytic streptococci (BHS) and viridans group streptococci (VGS), we compared the accuracy of MICroSTREP with that of the CLSI reference method. PMID: 21779193 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057197 Thu, 30 Jun 2011 23:00:00 +0100 5057197 http://www.medworm.com/index.php?rid=5057196&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779194%26dopt%3DAbstract Authors: Kim H, Lee Y, Moon HW, Lim CS, Lee K, Chong Y Clostridium difficile infection (CDI) has markedly risen and is associated with hypervirulent ribotype 027 outbreaks in North America and Europe since 2003. The aims of this study were to determine the prevalence of ribotype 027 among C. difficile isolates in Korea, to characterize the ribotype 027 isolates, and to determine the clinical severity of CDI in patients infected with these isolates. PMID: 21779194 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057196 Thu, 30 Jun 2011 23:00:00 +0100 5057196 http://www.medworm.com/index.php?rid=5057195&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779195%26dopt%3DAbstract Authors: Park CH, Jang MA, Ahn YH, Hwang YY, Ki CS, Lee NY Bacillus Calmette-Guërin (BCG) has been traditionally used as a vaccine against tuberculosis. Further, intravesical administration of BCG has been shown to be effective in treating bladder cancer. Although BCG contains a live attenuated strain of Mycobacterium bovis, complications such as M. bovis BCG infection caused by BCG administration are extremely rare. Here, we report a case of BCG infection occurring after intravesical BCG therapy. A 67-yr-old man presented with azotemia and weight loss. He had been diagnosed with bladder cancer 4 yr back, and had undergone transurethral resection of the bladder tumor and intravesical BCG (Tice strain) therapy at that time. An acid-fast bacterial strain was isolated from his urine samp... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057195 Thu, 30 Jun 2011 23:00:00 +0100 5057195 http://www.medworm.com/index.php?rid=5057194&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779196%26dopt%3DAbstract Authors: Choi J, Lee Y, Chung HS, Koo JS, Yong D, Kim YS, Lee K, Chong Y Phaeohyphomycosis is a subcutaneous infection caused by dark pigmented fungi, including fungi of the species Phaeoacremonium, Alternaria, Exophiala, and Pyrenochaeta. In August 2005, a 54-yr-old man who had received a renal transplant 5 yr ago was admitted to our hospital with a subcutaneous mass on the third finger of the right hand; the mass had been present for several months. He had been receiving immunosuppressive agents for several years. He underwent excision of the mass, which was followed by aspiration of the wound for bacterial and fungal cultures. Many fungal hyphae were observed on the histology slide treated with periodic acid-Schiff stain. A few white waxy colonies with a woolly texture grew on the S... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057194 Thu, 30 Jun 2011 23:00:00 +0100 5057194 http://www.medworm.com/index.php?rid=5057193&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779197%26dopt%3DAbstract Authors: Huh HJ, Park KJ, Jang JH, Lee M, Lee JH, Ahn YH, Kang CI, Ki CS, Lee NY Streptococcus suis infection is an emerging zoonosis in Asia. The most common disease manifestation is meningitis, which is often associated with hearing loss and cochleovestibular signs. S. suis infection in humans mainly occurs among risk groups that have frequent exposure to pigs or raw pork. Here, we report a case of S. suis meningitis in a 67-yr-old pig carcass handler, who presented with dizziness and sensorineural hearing loss followed by headaches. Gram-positive diplococci were isolated from cerebrospinal fluid (CSF) and blood cultures and showed gray-white colonies with α-hemolysis. S. suis was identified from CSF and blood cultures by using a Vitek 2 system (bioMérieux, France), API 20 STREP (b... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057193 Thu, 30 Jun 2011 23:00:00 +0100 5057193 http://www.medworm.com/index.php?rid=5057192&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779198%26dopt%3DAbstract The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs). PMID: 21779198 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057192 Thu, 30 Jun 2011 23:00:00 +0100 5057192 http://www.medworm.com/index.php?rid=5057191&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21779199%26dopt%3DAbstract Authors: Jung H, Ki CS, Koh WJ, Ahn KM, Lee SI, Kim JH, Ko JS, Seo JK, Cha SI, Lee ES, Kim JW Cystic fibrosis (CF) is one of the most common hereditary disorders among Caucasians. The most common mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been well established among Caucasian populations. In Koreans, however, there are very few cases of genetically confirmed CF thus far, and the spectrum of mutations seems quite different from that observed in Caucasians. PMID: 21779199 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=5057191 Thu, 30 Jun 2011 23:00:00 +0100 5057191 http://www.medworm.com/index.php?rid=4705103&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474978%26dopt%3DAbstract Authors: Boja ES, Rodriguez H Better biomarkers are urgently needed to cancer detection, diagnosis, and prognosis. While the genomics community is making significant advances in understanding the molecular basis of disease, proteomics will delineate the functional units of a cell, proteins and their intricate interaction network and signaling pathways for the underlying disease. Great progress has been made to characterize thousands of proteins qualitatively and quantitatively in complex biological systems by utilizing multi-dimensional sample fractionation strategies, mass spectrometry and protein microarrays. Comparative/quantitative analysis of high-quality clinical biospecimen (e.g., tissue and biofluids) of human cancer proteome landscape has the potential to reveal protein/peptid... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705103 Thu, 31 Mar 2011 23:00:00 +0100 4705103 http://www.medworm.com/index.php?rid=4705102&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474979%26dopt%3DAbstract Authors: Hawkins R Changes in renal function are one of the most common manifestations of severe illness. There is a clinical need to intervene early with proven treatments in patients with potentially deleterious changes in renal function. Unfortunately progress has been hindered by poor definitions of renal dysfunction and a lack of early biomarkers of renal injury. In recent years, the definitional problem has been addressed with the establishment of a new well-defined diagnostic entity, acute kidney injury (AKI), which encompasses the wide spectrum of kidney dysfunction, together with clearer definition and sub-classification of the cardio-renal syndromes. From the laboratory have emerged new biomarkers which allow early detection of AKI, including neutrophil gelatinase-associated ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705102 Thu, 31 Mar 2011 23:00:00 +0100 4705102 http://www.medworm.com/index.php?rid=4705101&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474980%26dopt%3DAbstract We describe the case of a 62-yr-old man who was diagnosed with erythroleukemia with a complex karyotype and achieved complete hematologic and cytogenetic remission after induction chemotherapy. However, 4 months after the initial diagnosis, he showed relapse with blasts showing a different morphology and immunophenotype and was diagnosed with precursor B-cell ALL. The relapsing precursor B-cell ALL presented with the same leukemic clones as the primary erythroleukemia. Cytogenetic analysis of his bone marrow (BM) at the time of the primary erythroleukemia showed complex karyotypic abnormalities, including monosomy 5 and monosomy 7. At relapse, his BM showed reemergence of these leukemic clones of complex karyotypic abnormalities with clonal switch. To our knowledge, this is the first case ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705101 Thu, 31 Mar 2011 23:00:00 +0100 4705101 http://www.medworm.com/index.php?rid=4705100&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474981%26dopt%3DAbstract We present a rare case of microgranular variant acute promyelocytic leukemia (APL) associated with ider(17)(q10)t(15;17)(q22;q12) of an old-age patient. The initial chromosome study showed a 46,XX,del(6)(?q21q25),der(15)t(15;17)(q22;q12),ider(17)(q10)t(15;17)[10]/47,sl,+ider(17)(q10)t(15;17)[3]/46,XX[16]. FISH signals from a dual color dual fusion translocation PML-RARA probe were consistent with the results of conventional cytogenetics. Because of the rarity of ider(17)(q10)t(15;17) in microgranular APL, further studies on both gene dosage effect of this chromosomal abnormality and the influence of ider(17)(q10)t(15;17) on clinical features such as prognosis, survival, and treatment response of APL cases are recommended. PMID: 21474981 [PubMed - in process] (Source: The Korean Journal... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705100 Thu, 31 Mar 2011 23:00:00 +0100 4705100 http://www.medworm.com/index.php?rid=4705099&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474982%26dopt%3DAbstract Authors: Park KJ, Chung HS, Kim SR, Kim HJ, Han JY, Lee SY Clopidogrel has been widely used to prevent recurrent ischemia in patients with acute coronary syndrome (ACS). However, inter-individual variability in response to clopidogrel has been a problem in the clinical setting. The aim of the present study was to investigate the frequency of clopidogrel resistance and to determine the clinical, pharmacokinetic, and pharmacogenetic factors for clopidogrel resistance in Korean patients with ACS. PMID: 21474982 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705099 Thu, 31 Mar 2011 23:00:00 +0100 4705099 http://www.medworm.com/index.php?rid=4705098&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474983%26dopt%3DAbstract Authors: Yadav BK, Gupta RK, Gyawali P, Shrestha R, Poudel B, Sigdel M, Jha B Various synthetic progestogens that are used as contraceptives have been reported to influence lipid and lipoprotein fractions differently. Depo-medroxyprogesterone acetate (DMPA), a synthetic progestogen, is used by Nepalese women as a contraceptive agent. Our study aims to determine the effects of long-term use of DMPA on lipid metabolism. We performed this study on 60 healthy Nepalese women who had been using DMPA for more than 2 yr and age- and weight-matched control subjects who were not using hormonal contraceptives. Fasting blood samples were collected from the subjects for the estimation of total cholesterol (TC) and triglyceride (TG) levels, and the levels of high-density lipoprotein cholesterol (HDL... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705098 Thu, 31 Mar 2011 23:00:00 +0100 4705098 http://www.medworm.com/index.php?rid=4705097&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474984%26dopt%3DAbstract Authors: Cho SY, Lee A, Lee HJ, Suh JT PMID: 21474984 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705097 Thu, 31 Mar 2011 23:00:00 +0100 4705097 http://www.medworm.com/index.php?rid=4705096&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474985%26dopt%3DAbstract Authors: Shin JH, Song SA, Kim MN, Lee NY, Kim EC, Kim S, Koo SH, Ryoo NH, Kim JS, Cho JH Optimal blood culture performance is critical for successful diagnosis and treatment of sepsis. To understand the status of blood culture, we investigated several aspects of the procedure at 9 university hospitals. PMID: 21474985 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705096 Thu, 31 Mar 2011 23:00:00 +0100 4705096 http://www.medworm.com/index.php?rid=4705095&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474986%26dopt%3DAbstract Authors: Myoung Y, Shin JH, Lee JS, Kim SH, Shin MG, Suh SP, Ryang DW We evaluated the efficacy of multilocus sequence typing (MLST) for assessing the genetic relationship among Candida albicans isolates from patients with candidemia in a hospital setting. PMID: 21474986 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705095 Thu, 31 Mar 2011 23:00:00 +0100 4705095 http://www.medworm.com/index.php?rid=4705094&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474987%26dopt%3DAbstract We report the case of a patient with septic arthritis and bacteremia caused by S. suis. Cases in which S. suis is isolated from the joint fluid are very rare, and to the best of our knowledge, this is first case report of S. suis infection in Korea. The identity of this organism was confirmed by phenotypic characterization and 16S rRNA sequence analysis. An 81-yr-old Korean woman who presented with fever, arthralgia, and headache was admitted to a secondary referral center in Korea. Culture of aspirated joint fluid and blood samples showed the growth of S. suis biotype II, which was identified by the Vitek2 GPI and API 20 Strep systems (bioMérieux, USA), and this organism was susceptible to penicillin G and vancomycin. The 16S rRNA sequences of the blood culture isolates showed 99% homolo... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705094 Thu, 31 Mar 2011 23:00:00 +0100 4705094 http://www.medworm.com/index.php?rid=4705093&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474988%26dopt%3DAbstract Authors: Won D, An D, Kim MN, Lee YS A 42-yr-old man with hepatitis B virus associated liver cirrhosis was admitted to the emergency room because of multiple seizures, a history of chills and myalgia over the previous 2 weeks, and 3 days of melena. He was febrile with a temperature of 38.0°C. There were no symptoms and signs related to the genitourinary system, skin, or joints. Three sets of blood cultures were obtained and oxidase-positive, gram-negative diplococci were detected after 25.9-26.9 hr of incubation in all aerobic vials. The organism was positive for catalase and oxidase, and was identified as Neisseria gonorrhoeae, using a Vitek Neisseria-Haemophilus Identification card (bioMérieux Vitek, Inc., USA). Further, 16S rRNA sequencing of this isolate revealed a 99.9% homology... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705093 Thu, 31 Mar 2011 23:00:00 +0100 4705093 http://www.medworm.com/index.php?rid=4705092&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474989%26dopt%3DAbstract Authors: Chung S, Kim JS, Seo SW, Ra EK, Joo SI, Kim SY, Park SS, Kim EC Propionibacterium acnes is a gram-positive anaerobic bacillus and a normal inhabitant of the skin. Although it is often considered a contaminant of blood cultures, it can occasionally cause serious infections, including postoperative central nervous system infections. Here, we report the case of a 70-yr-old man who developed a large cerebral abscess caused by P. acnes 13 months after neurosurgery. Immediate gram staining of the pus from his brain revealed the presence of gram-positive coccobacilli. However, colony growth was observed only after 5 days of culture. Therefore, we performed 16S rRNA gene sequencing of the pus specimen. The isolate was identified as P. acnes. The colonies developed 9 days after the ini... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705092 Thu, 31 Mar 2011 23:00:00 +0100 4705092 http://www.medworm.com/index.php?rid=4705091&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474990%26dopt%3DAbstract We report a case of AML with a rare 3-way translocation involving chromosomes 1, 9, and 11: t(1;9;11)(p34.2;p22;q23). A 3-yr-old Korean girl presented with a 5-day history of fever. A diagnosis of AML was made on the basis of the morphological evaluation and immunophenotyping of bone marrow specimens. Flow cytometric immunophenotyping showed blasts positive for myeloid lineage markers and aberrant CD19 expression. Karyotypic analysis showed 46,XX,t(1;9;11)(p34.2;p22;q23) in 19 of the 20 cells analyzed. This abnormality was involved in MLL/MLLT3 rearrangement, which was confirmed by qualitative multiplex reverse transcription-PCR and interphase FISH. She achieved morphological and cytogenetic remission after 1 month of chemotherapy and remained event-free for 6 months. Four cases of t(1;9;1... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4705091 Thu, 31 Mar 2011 23:00:00 +0100 4705091 http://www.medworm.com/index.php?rid=4409779&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239863%26dopt%3DAbstract Authors: Kim SY, Kim HK, Han KS, Kim I, Yoon SS, Park S, Kim BK Heparin-induced thrombocytopenia (HIT) is an adverse drug reaction caused by antibodies to the heparin/platelet factor 4 (PF4) complex, resulting in thrombocytopenia and prothrombotic state. HIT diagnosis is challenging and depends on clinical presentation and laboratory tests. We investigated the usefulness of clinical scores and heparin/PF4 ELISA optical density (OD) as a diagnostic marker and thrombosis predictor in HIT. PMID: 21239863 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409779 Sat, 01 Jan 2011 00:00:00 +0100 4409779 http://www.medworm.com/index.php?rid=4409778&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239864%26dopt%3DAbstract We report 2 cases of APL presenting with CNS involvement. A 43-yr-old woman presented with easy bruising and stuporous mentality. Her complete blood count (CBC) revealed leukocytosis with increased blasts. Bone marrow (BM) analysis was carried out, and the diagnosis of APL was confirmed. This was done by cytogenetic analysis and demonstration of PML-RARα rearrangement by reverse transcriptase PCR in the BM cells. A lumbar puncture was performed to investigate the cause of her stuporous mentality, and her cerebrospinal fluid (CSF) analysis revealed 97% leukemic promyelocytes. Despite systemic and CNS therapy, she died due to septic shock by infection and rapid disease progression only 3 days after her admission. Another patient, a 3-yr-old girl, presented with easy bruising and epistaxis, ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409778 Sat, 01 Jan 2011 00:00:00 +0100 4409778 http://www.medworm.com/index.php?rid=4409777&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239865%26dopt%3DAbstract Authors: Yoo BJ, Nam MH, Sung HJ, Lim CS, Lee CK, Cho YJ, Lee KN, Yoon SY Therapy-related ALL (t-ALL) is a rare secondary leukemia that develops after chemotherapy and/or radiotherapy for primary malignancies. Chromosomal 11q23 abnormalities are the most common karyotypic alterations in t-ALL. The t(11;19)(q23;p13) aberration is extremely rare and has not been confirmed at the molecular genetic level. Here, we report a case of t-ALL with t(11;19)(q23;p13.3) and MLL-MLLT1 (alias ENL) gene rearrangement confirmed by cytogenetic analysis, multiplex reverse transcription-PCR (multiplex RT-PCR), and DNA sequencing in a patient who had undergone treatment for breast cancer. A 40-yr-old woman developed acute leukemia 15 months after undergoing 6 cycles of adjuvant chemotherapy (doxorubicin 60... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409777 Sat, 01 Jan 2011 00:00:00 +0100 4409777 http://www.medworm.com/index.php?rid=4409776&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239866%26dopt%3DAbstract Authors: Kim do Y, Kim YS, Huh HJ, Choi JS, Yeo JS, Kwak BS, Chae SL Monoclonal gammopathy occurs in one-third of the patients with mucosa-associated lymphoid tissue lymphoma (MALT lymphoma). However, monoclonal gammopathy has been rarely reported in Korea. Paraprotenemia accompanying MALT lymphoma is strongly correlated with involvement of the bone marrow, and this involvement leads to the progression of the disease. Here, we present a case of a 66-yr-old man diagnosed with IgM monoclonal gammopathy and stage IV extranodal marginal zone lymphoma of the small intestine, with the involvement of the bone marrow. PMID: 21239866 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409776 Sat, 01 Jan 2011 00:00:00 +0100 4409776 http://www.medworm.com/index.php?rid=4409775&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239867%26dopt%3DAbstract Authors: Kim S, Yun YM, Hur M, Moon HW, Kim JQ Insulin assays are affected by varying degrees of interference from anti-insulin antibodies (IAs) and by cross-reactivity with recombinant insulin analogues. We evaluated the usefulness of the E170 insulin assay by assessing IA effects and cross-reactivity with 2 analogues. PMID: 21239867 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409775 Sat, 01 Jan 2011 00:00:00 +0100 4409775 http://www.medworm.com/index.php?rid=4409774&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239868%26dopt%3DAbstract Authors: Kim T, Yi J, Hong KH, Park JS, Kim EC Various virulence factors and superantigens are encoded by mobile genetic elements. The relationship between clonal background and virulence factors differs in different geographic regions. We compared the distribution and relationship of spa types and virulence genes among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from a tertiary hospital in 2000-01 and 2007-08. PMID: 21239868 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409774 Sat, 01 Jan 2011 00:00:00 +0100 4409774 http://www.medworm.com/index.php?rid=4409773&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239869%26dopt%3DAbstract This study applied SNP and MIRU-VNTR analyses for molecular strain typing of Mycobacterium tuberculosis isolates collected throughout Korea. PMID: 21239869 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409773 Sat, 01 Jan 2011 00:00:00 +0100 4409773 http://www.medworm.com/index.php?rid=4409772&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239870%26dopt%3DAbstract We report a case of brain tuberculoma where the tissue sample was contaminated with root hair derived from tap water; the presence of this root hair, which mimicked a larva, led to diagnostic confusion. Therefore, tap water should be considered a source of root hair and vegetable matter. PMID: 21239870 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409772 Sat, 01 Jan 2011 00:00:00 +0100 4409772 http://www.medworm.com/index.php?rid=4409771&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239871%26dopt%3DAbstract In this report, we present a case of bacteremia caused by C. amycolatum with a novel mutation in the gyrA gene that confers high-level quinolone resistance to the organism. PMID: 21239871 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409771 Sat, 01 Jan 2011 00:00:00 +0100 4409771 http://www.medworm.com/index.php?rid=4409770&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239872%26dopt%3DAbstract We report the case of a female infant with der(17)t(12;17)(q24.33;p13.3)pat caused by an unbalanced segregation of the parental balanced translocation of 17p with other chromosomes. The proband presented with facial dysmorphism, arthrogryposis, and intrauterine growth retardation. Most cases of Miller-Dieker syndrome have a de novo deletion involving 17p13.3. When Miller-Dieker syndrome is caused by an unbalanced translocation, mild-to-severe phenotypes occur according to the extension of the involved partner chromosome. However, a pure partial monosomy derived from a paternal balanced translocation is relatively rare. In this case, the submicroscopic cryptic deletion in the proband was initially elucidated by FISH, and karyotype analysis did not reveal additional chromosome abnormalities ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409770 Sat, 01 Jan 2011 00:00:00 +0100 4409770 http://www.medworm.com/index.php?rid=4409769&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21239873%26dopt%3DAbstract This report presents evidence of the biochemical and molecular features of MCADD in Korean patients and, to the best of our knowledge, this is the first report of the c.461T⟩G mutation in the ACADM gene. PMID: 21239873 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4409769 Sat, 01 Jan 2011 00:00:00 +0100 4409769 http://www.medworm.com/index.php?rid=4278387&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157134%26dopt%3DAbstract CONCLUSIONS: Because of decreased number of mAbs needed, it is possible that acute leukemia panel is designed to include all mAbs required to diagnose MPAL according to 2008 WHO classification. When diagnosing MPAL, it is critical to figure out positivity in either cMPO or CD19, and AML expressing more than 2 lymphoid antigens are considered as MPAL. PMID: 21157134 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278387 Wed, 01 Dec 2010 00:00:00 +0100 4278387 http://www.medworm.com/index.php?rid=4278386&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157135%26dopt%3DAbstract CONCLUSIONS: MRD detection by flow cytometric assay of cross-lineage antigen expression would be useful in predicting treatment outcome in patients with ALL rather than AML. We expect that the establishment of the standardization of methods, time to test or antibody combination would be achieved through further trials in this country. PMID: 21157135 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278386 Wed, 01 Dec 2010 00:00:00 +0100 4278386 http://www.medworm.com/index.php?rid=4278385&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157136%26dopt%3DAbstract In this study, we evaluated the 5-part differential patterns of blasts using automated analyzers to determine if they can differentiate among blast types. PMID: 21157136 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278385 Wed, 01 Dec 2010 00:00:00 +0100 4278385 http://www.medworm.com/index.php?rid=4278384&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157137%26dopt%3DAbstract CONCLUSIONS: Non-gastric MALT lymphoma patients showed worse prognosis compared to gastric cases. As for remission rates in patients with gastric MALT lymphoma, successful H. pylori eradication therapy could be a good prognostic factor even if H. pylori infection was present at diagnosis. PMID: 21157137 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278384 Wed, 01 Dec 2010 00:00:00 +0100 4278384 http://www.medworm.com/index.php?rid=4278383&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157138%26dopt%3DAbstract CONCLUSIONS: Higher levels of EBV viral load in donors appear to be associated with EBV transmission to recipients in HSCT. EBV real-time quantitative PCR may be needed for screening EBV DNA level in HSCT donors. PMID: 21157138 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278383 Wed, 01 Dec 2010 00:00:00 +0100 4278383 http://www.medworm.com/index.php?rid=4278382&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157139%26dopt%3DAbstract CONCLUSIONS: We proposed a basic guideline for MSR. This guideline would be helpful to hematology laboratories for their daily operation and providing more rapid and accurate CBC and WBC differential results. PMID: 21157139 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278382 Wed, 01 Dec 2010 00:00:00 +0100 4278382 http://www.medworm.com/index.php?rid=4278381&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157140%26dopt%3DAbstract CONCLUSIONS: Frequencies of V617F are similar to reported results. JAK2 exon 12 mutations may be rare and c.1641+179_183del5 may influence the occurrence of MPN in Korean patients with V6 17F-negative MPN. PMID: 21157140 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278381 Wed, 01 Dec 2010 00:00:00 +0100 4278381 http://www.medworm.com/index.php?rid=4278380&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157141%26dopt%3DAbstract CONCLUSIONS: The incidence of LON following rituximab therapy was 15.3% in Korean patients with DLBCL. Although there are several hypotheses about the causative mechanisms of LON, we suggest that maturation arrest at the promyelocyte stage of granulopoiesis may be one of the mechanisms involved in the development of LON. PMID: 21157141 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278380 Wed, 01 Dec 2010 00:00:00 +0100 4278380 http://www.medworm.com/index.php?rid=4278376&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157142%26dopt%3DAbstract Authors: Park SH, Ahn JY, Seo YH, Park PH, Kim KH, Song YH, Jeong JH, Lee JH Crystal-storing histiocytosis (CSH) is a rare event observed in association with lymphoproliferative diseases, and mainly occurrs in plasma cell dyscrasias. It is presumed to be an intra-lysosomal accumulation of the secreted paraproteins. Crystal formation can be seen inside histiocyte-like cells with phagocytosed crystalline inclusions in the bone marrow and extramedullary sites. CSH is a rare morphological entity with poor prognostic implications and may be confused with Gaucher or pseudo-Gaucher cells. Herein we report a case of non-secretory myeloma associated with CSH showing a poor clinical course. A 79-yr-old male presenting with dizziness was evaluated in hematology department for anemia. Laboratory t... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278376 Wed, 01 Dec 2010 00:00:00 +0100 4278376 http://www.medworm.com/index.php?rid=4278375&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157143%26dopt%3DAbstract We describe the first case of ider(9)(q10)t(9;22) in combination with TCF3-PBX1 negative t(1;19). The patient underwent imatinib therapy in addition to intensive chemotherapy, but failed to achieve remission. PMID: 21157143 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278375 Wed, 01 Dec 2010 00:00:00 +0100 4278375 http://www.medworm.com/index.php?rid=4278374&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157144%26dopt%3DAbstract We report the first case of liver transplant recipient with parvovirus B19-induced pure red cell aplasia in Korea. A 57-yr-old female patient with hepatocellular carcinoma due to hepatitis C virus received a liver transplantation. Two months later, anemia developed and she received periodic red blood cell transfusions. However, chronic anemia persisted and bone marrow examination was performed 8 months after transplantation. Bone marrow aspiration smears showed markedly reduced erythroid precursors with atypical giant pronormoblasts and nuclear remnants with viral inclusions, and characteristic lantern cells were observed in biopsy sections. In addition, parvovirus B19 DNA PCR was positive. She was diagnosed as parvovirus B19-induced pure red cell aplasia and her anemia was improved follow... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278374 Wed, 01 Dec 2010 00:00:00 +0100 4278374 http://www.medworm.com/index.php?rid=4278373&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157145%26dopt%3DAbstract We report a case of blastic crisis of CML with both t(3;3)(q21;q26.2) and t(9;22)(q34;q11.2) and associated severe multilineage dysplasia. The patient showed a poor response to imatinib, dasatinib and aggressive induction therapy. When both t(3;3)(q21;q26.2) and t(9;22)(q34;q11.2) are observed in cases of leukemia with increased blasts, they are best considered as aggressive phases of CML with t(3;3)(q21;q26.2), rather than AML with t(9;22)(q34;q11.2) by 2008 WHO classification. PMID: 21157145 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278373 Wed, 01 Dec 2010 00:00:00 +0100 4278373 http://www.medworm.com/index.php?rid=4278372&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157146%26dopt%3DAbstract This study aimed to assess the clinical significance of the sIL-2Rα level in non-Hodgkin's lymphoma (NHL). We used ELISA to measure the sIL-2Rα levels in 48 newly diagnosed and untreated patients with NHL and evaluated the correlation between the sIL-2Rα levels and clinical characteristics and the International Prognostic Index (IPI). We monitored serum sIL-2Rα in 7 patients to compare the changes in their clinical progress with these levels. High levels of serum sIL-2Rα (≥2,000 U/mL) correlated well with parameters defining the high risk group according to the IPI, i.e., high tumor burden at diagnosis (stage III+IV) and lactate dehydrogenase ≥472 U/L. The levels were also associated with B symptoms, bone marrow involvement, and poor response to therapy. The sIL-2Rα level decreas... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278372 Wed, 01 Dec 2010 00:00:00 +0100 4278372 http://www.medworm.com/index.php?rid=4278371&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157147%26dopt%3DAbstract CONCLUSIONS: We suggest that absolute, non-corrected GFR and GFR estimate be preferred in daily practice. The absolute, non-corrected GFR and GFR estimate are considered helpful for patients with eGFR≤60 mL/min/1.73 m(2). We also recommend the clinical use of the new formulae, SMI-adjusted CG and SMI×3.4/SCr (BSA-unadjusted). PMID: 21157147 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278371 Wed, 01 Dec 2010 00:00:00 +0100 4278371 http://www.medworm.com/index.php?rid=4278370&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157148%26dopt%3DAbstract CONCLUSIONS: In this study, the most common types of class A ESBLs identified were CTX-M-15-like in E. coli and SHV-12-like in K. pneumoniae. PMID: 21157148 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278370 Wed, 01 Dec 2010 00:00:00 +0100 4278370 http://www.medworm.com/index.php?rid=4278369&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157149%26dopt%3DAbstract CONCLUSIONS: The Real-Q Enterovirus Quantification kit showed excellent linearity and high reliability with a broad reportable range. It showed good detection rate when used with clinical specimens and also showed a high concordance with the conventional method. Therefore, this assay would be clinically useful not only in diagnosis of aseptic meningitis but also in differential diagnosis of infantile sepsis. PMID: 21157149 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278369 Wed, 01 Dec 2010 00:00:00 +0100 4278369 http://www.medworm.com/index.php?rid=4278368&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157150%26dopt%3DAbstract CONCLUSIONS: For VRE surveillance, culturing the isolates in chromID VRE after broth enrichment appears to be an accurate, rapid, and easy method for routine screening test. Multiplex PCR is relatively expensive and needs skilled techniques for detecting VRE, but it can be an auxiliary tool for rapid detection of genotype during a VRE outbreak. PMID: 21157150 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278368 Wed, 01 Dec 2010 00:00:00 +0100 4278368 http://www.medworm.com/index.php?rid=4278367&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157151%26dopt%3DAbstract CONCLUSIONS: PBC28 can be appropriate susceptibility testing of S. aureus, including MRSA detection and ICD. However, the lower-range vancomycin MIC test was not reproducible enough to reliably differentiate MIC of 2 µg/mL from MIC≤1 µg/mL. PMID: 21157151 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278367 Wed, 01 Dec 2010 00:00:00 +0100 4278367 http://www.medworm.com/index.php?rid=4278366&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157152%26dopt%3DAbstract CONCLUSIONS: We performed epidemiological analysis of norovirus infection using ELISA assay. Reverse transcription-PCR indicated higher prevalence of norovirus infection as compared with that reported before 2007. Further studies are warranted to examine the changes observed in the epidemic period of 2009. PMID: 21157152 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278366 Wed, 01 Dec 2010 00:00:00 +0100 4278366 http://www.medworm.com/index.php?rid=4278365&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157153%26dopt%3DAbstract CONCLUSIONS: The ARCHITECT HCV Ag assay was good in sensitivity, precision, and linearity and its results well correlated with HCV RNA levels. This assay could be used as a good marker of viral replication for monitoring the therapy response in chronically HCV infected patients. PMID: 21157153 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278365 Wed, 01 Dec 2010 00:00:00 +0100 4278365 http://www.medworm.com/index.php?rid=4278364&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157154%26dopt%3DAbstract CONCLUSIONS: Total IgE appears to be useful in predicting positive results in allergen specific IgE tests to common allergens. The specific IgEs to D. farinae and D. pteronyssinus showed good correlation with total IgE. However, for other allergens, significant differences were observed among different test kits, and the standardization of allergens in multiple allergen simultaneous tests is needed. PMID: 21157154 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278364 Wed, 01 Dec 2010 00:00:00 +0100 4278364 http://www.medworm.com/index.php?rid=4278363&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157155%26dopt%3DAbstract CONCLUSIONS: The error rate of HLA RT results of unrelated stem cell donors in the Korean registry was similar to those in the foreign registries, and has decreased in the recent searches following the change in the typing method from serological to DNA typing. PMID: 21157155 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278363 Wed, 01 Dec 2010 00:00:00 +0100 4278363 http://www.medworm.com/index.php?rid=4278362&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157156%26dopt%3DAbstract CONCLUSIONS: Six different assays showed various performances depending on the methods and cut-off values used. Except IB, the other five assays can be used for the detection of anti-dsDNA. PMID: 21157156 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278362 Wed, 01 Dec 2010 00:00:00 +0100 4278362 http://www.medworm.com/index.php?rid=4278361&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157157%26dopt%3DAbstract CONCLUSIONS: The information obtained in this study can be used as basic data for Koreans in the fields of organ transplantation, disease association, and anthropologic studies. PMID: 21157157 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278361 Wed, 01 Dec 2010 00:00:00 +0100 4278361 http://www.medworm.com/index.php?rid=4278358&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157158%26dopt%3DAbstract CONCLUSIONS: Frozen-thawed-deglycerolized RBCs used in RBC deformability test showed satisfactory within-run and between-run precisions and stability for one week after thawing, and may be used as QC materials for this test. PMID: 21157158 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278358 Wed, 01 Dec 2010 00:00:00 +0100 4278358 http://www.medworm.com/index.php?rid=4278357&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157159%26dopt%3DAbstract CONCLUSIONS: These data indicate that RYR1 is a main candidate gene in Korean MH families, and that comprehensive screening of the entire coding sequence of the RYR1 gene is necessary for molecular genetic investigations in MH-susceptible individuals, owing to the presence of RYR1 mutations or variations outside of the hotspot region. PMID: 21157159 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278357 Wed, 01 Dec 2010 00:00:00 +0100 4278357 http://www.medworm.com/index.php?rid=4278356&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157160%26dopt%3DAbstract Authors: Kim MK, Mun YC, Seong CM, Chung WS, Huh J A variant Philadelphia chromosome (Ph) is generated from translocation of one or more partner chromosomes in addition to chromosomes 9 and 22. We have described the cases of 2 patients bearing variant Ph detected by interphase FISH but not detected by G-banded karyotyping and metaphase FISH. FISH was performed using BCR/ABL dual color dual fusion translocation probes (Abbott Molecular, USA). A 52-year-old man was diagnosed with acute leukemia of mixed phenotype. G-banded karyotyping showed 46,XY,t(9;22)(q34;q11.2)[12]/47,idem,+der(22)t(9;22)[5]/46,XY[3]. Interphase FISH revealed nuc ish(ABL1,BCR)×3(ABL1 con BCR×2)[329/450]/(ABL1,BCR)×4(ABL1 con BCR×3)[5/450]/(AL1,BCR)×3(ABL1 con BCR×1)[44/450]. Metaphase FISH showed ish (9;22)(AB... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278356 Wed, 01 Dec 2010 00:00:00 +0100 4278356 http://www.medworm.com/index.php?rid=4278355&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157161%26dopt%3DAbstract CONCLUSIONS: The amount and integrity of nucleic acids extracted from stored blood samples are potential indicators that can be used for quality control. A guideline for the quality assessment of stored blood samples in a biobank is urgently needed. PMID: 21157161 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278355 Wed, 01 Dec 2010 00:00:00 +0100 4278355 http://www.medworm.com/index.php?rid=4278350&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21157162%26dopt%3DAbstract CONCLUSIONS: In the first Korean FOBT EQA, commercially available EQA materials were proven to be stable. Continuation of the EQA program and further education of laboratory personnel are needed to reduce inconsistency in results. Further, the test kit, procedures, and result reports must be standardized. PMID: 21157162 [PubMed - as supplied by publisher] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4278350 Wed, 01 Dec 2010 00:00:00 +0100 4278350 http://www.medworm.com/index.php?rid=4045556&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890075%26dopt%3DAbstract CONCLUSIONS: A rapid and inexpensive automated measurement of IPF can be integrated as a standard parameter to evaluate the thrombopoietic state of the bone marrow. This study determined the reference intervals of IPF from a large population of healthy individuals, including children. Further studies are needed to establish the clinical utility of IPF. PMID: 20890075 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045556 Thu, 30 Sep 2010 23:00:00 +0100 4045556 http://www.medworm.com/index.php?rid=4045551&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890076%26dopt%3DAbstract CONCLUSIONS: The clinical usefulness of the different assays for the correct classification of patients in terms of antiplatelet resistance remains unclear. Further studies are required to determine the best method for correlating the occurrences of adverse ischemic events. PMID: 20890076 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045551 Thu, 30 Sep 2010 23:00:00 +0100 4045551 http://www.medworm.com/index.php?rid=4045544&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890077%26dopt%3DAbstract In this study, we performed pyrosequencing to quantify the methylation level of HOXA5 genes in the bone marrow samples obtained from 50 patients with AML and 19 normal controls. The methylation percentage of HOXA5 in AML patients (median=65.4%, interquartile range=35.9-72.3%) was higher than that of HOXA5 in control patients (median=43.1%, interquartile range=36.7-49.6%, Mann-Whitney U test, P=0.012). The patients of the AML group who had a high methylation percentage (>70%) had a good prognosis with a 3-yr overall survival (OS) of 82.5%, whereas the patients with a low methylation percentage (≤70%) showed a 3-yr OS of 40.5% (P=0.048). Cox proportional hazards regression showed that the methylation percentages of HOXA5 were independently associated with the 3-yr OS of AML patients, re... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045544 Thu, 30 Sep 2010 23:00:00 +0100 4045544 http://www.medworm.com/index.php?rid=4045537&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890078%26dopt%3DAbstract Authors: Kim HJ, Jang JH, Yoo EH, Kim HJ, Ki CS, Kim JW, Kim SH JAK2 V617F and MPL W515L/K mutations have been reported in approximately 50% and 5% of the patients with essential thrombocythemia (ET), respectively. We investigated the frequency of MPL W515L/K mutations in a series of consecutive patients with ET and post-essential thrombocythemia myelofibrosis (post-ET MF). The study subjects were 63 patients diagnosed either with ET (N=59) or post-ET MF (N=4) at our institution between June 2006 and February 2010. Among them, 35 (55.6%) had the JAK2 V617F mutation. MPL W515L/K mutations were detected by direct sequencing analyses of exon 10, and 2 patients were found to harbor the following MPL mutations: W515L in 1 patient with ET and W515K in 1 patient with post-ET MF. Neither of th... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045537 Thu, 30 Sep 2010 23:00:00 +0100 4045537 http://www.medworm.com/index.php?rid=4045528&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890079%26dopt%3DAbstract CONCLUSIONS: The CDT test using capillary EP showed good performance, and this method has several advantages such as automation and detection of variant forms. Thus, CDT can be a more useful marker than GGT for monitoring alcohol abstinence, especially in patients with liver disease. PMID: 20890079 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045528 Thu, 30 Sep 2010 23:00:00 +0100 4045528 http://www.medworm.com/index.php?rid=4045518&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890080%26dopt%3DAbstract CONCLUSIONS: The erythromycin resistance rate of GAS isolated from children with acute pharyngitis was significantly lower in 2009 (4.6%) than in 2002 (44.8%). We observed a remarkable change in the distribution of emm genotypes during the 7-yr period. The significant decline in erythromycin resistance in 2009 might be associated with a prominent decrease in the resistant genotype emm12 (3.4% in 2009 vs. 28.0% in 2002) rather than restriction of macrolide use. PMID: 20890080 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045518 Thu, 30 Sep 2010 23:00:00 +0100 4045518 http://www.medworm.com/index.php?rid=4045510&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890081%26dopt%3DAbstract CONCLUSIONS: This is the first nationwide study on the toxin status, including PCR ribotyping and antimicrobial resistance, of C. difficile isolates in Korea. The prevalence of A-B+ strains was 25.7%, much higher than that reported from other countries. Binary toxin-producing strains accounted for 7.1% of all strains, which was not rare in Korea. The most prevalent ribotype was ribotype 017, and all A-B+ strains showed this pattern. We did not isolate strains with decreased susceptibility to metronidazole or vancomycin. PMID: 20890081 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045510 Thu, 30 Sep 2010 23:00:00 +0100 4045510 http://www.medworm.com/index.php?rid=4045503&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890082%26dopt%3DAbstract CONCLUSIONS: Of the 35 MDR A. baumannii isolates, 26 (74.3%) from both outbreak and sporadic cases possessed at least 4 of the 7 antibiotic resistance gene determinants that give rise to the MDR phenotype. The co-occurrence of several resistance determinants may present a significant threat. PMID: 20890082 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045503 Thu, 30 Sep 2010 23:00:00 +0100 4045503 http://www.medworm.com/index.php?rid=4045491&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890083%26dopt%3DAbstract Authors: Kim SJ, Kim CM, Shin SH We identified 6 sucrose-fermenting Vibrio vulnificus strains and examined their virulence characteristics. They were all encapsulated, motile, capable of producing toxins and utilizing transferrin-bound iron, cytotoxic to cultured cells, and virulent enough to kill mice. They could be definitely identified only by genetic identification methods such as PCR, and not by conventional culture-based identification methods such as API 20E (bioMérieux, France). These results indicate that it is essential to adopt genetic approaches as early as possible in order to avoid misdiagnosis of such strains, especially in clinical situations. PMID: 20890083 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045491 Thu, 30 Sep 2010 23:00:00 +0100 4045491 http://www.medworm.com/index.php?rid=4045481&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890084%26dopt%3DAbstract We report the first case of HDNF caused by anti-Jr(a) alloimmunization in twins in Korea. A 33-yr-old nulliparous woman with no history of transfusion or amniocentesis was admitted at the 32nd week of gestation because of vaginal bleeding caused by placenta previa. Anti-Jr(a) antibodies were detected in a routine laboratory examination. An emergency cesarean section was performed at the 34th week of gestation, and 2 premature infant twins were delivered. Laboratory examination showed positive direct antiglobulin test and Jr(a+) phenotype in the red blood cells and the presence of anti-Jr(a) antibodies in the serum in both neonates. The infants underwent phototherapy for neonatal jaundice; this was followed by conservative management. They showed no further complications and were discharged... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045481 Thu, 30 Sep 2010 23:00:00 +0100 4045481 http://www.medworm.com/index.php?rid=4045474&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890085%26dopt%3DAbstract This study identified a novel mutation in the ARSA gene, related to a late-infantile form of MLD with a lethal clinical course and suggested that molecular diagnosis of patients may be useful in early diagnosis and for deciding intervention measures for their family members. PMID: 20890085 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045474 Thu, 30 Sep 2010 23:00:00 +0100 4045474 http://www.medworm.com/index.php?rid=4045461&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20890086%26dopt%3DAbstract We present a case of blood chimerism in a dizygotic dichorionic twin pregnancy. The female twin was delivered at 34 weeks of gestation, and the male twin was stillborn. Pathologic examination confirmed dichorionic diamniotic placentas. The karyotype of the female child was obtained using peripheral blood sample, and it revealed a mixture of 46,XX and 46,XY cells (chi 46,XY[13]/46,XX[7]). FISH analysis performed on the buccal cells by using CEP X/Y probe (Abbott Molecular Inc., USA) revealed 100% XX signals (nuc ish Xcen(DXZ1x2)[500]). Gross examination of the external genitalia and abdominal ultrasonography revealed no definitive abnormal findings in relation to sex differentiation. When XX/XY chimerism is present in blood lymphocytes, careful examination of external genitalia and reproduc... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=4045461 Thu, 30 Sep 2010 23:00:00 +0100 4045461 http://www.medworm.com/index.php?rid=3934755&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805702%26dopt%3DAbstract We report a case of AML with del(16)(q22) which showed a complex karyotype, absence of eosinophilia in bone marrow study and a poor response to chemotherapy. PMID: 20805702 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934755 Sat, 31 Jul 2010 23:00:00 +0100 3934755 http://www.medworm.com/index.php?rid=3934754&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805703%26dopt%3DAbstract We report a case of central nervous system myelomatosis with complex chromosome aberrations in a 62-yr-old female patient, who had previously been diagnosed as multiple myeloma. Fluorescent in situ hybridization revealed 13q deletion, p53 gene deletion and IGH/FGFR3 rearrangement and chromosomal study showed complex chromosome aberrations. After four cycles of chemotherapy, the patient was admitted to the hematology department with severe headache. Plasma cells were found in the cerebrospinal fluid (CSF), and CSF immunoelectrophoresis revealed abnormal precipitin arcs against anti-IgG and anti-lambda antisera. She was given systemic chemotherapy and eight courses of intrathecal chemotherapy, which cleared plasma cells in the CSF. Two months later, she was given autologous stem cell transpl... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934754 Sat, 31 Jul 2010 23:00:00 +0100 3934754 http://www.medworm.com/index.php?rid=3934753&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805704%26dopt%3DAbstract We report a case of hereditary spherocytosis in a 12-yr-old male child, in whom flow-assisted diagnosis was made. In this case, diagnosis was not determined because routine laboratory workups for hereditary spherocytosis yielded discrepant RESULTS: positive osmotic fragility test, positive direct antiglobulin test, and normal result in the red cell membrane protein sodium dodecyl succinimide polyacrylamide gel electrophoresis. However, all flow cytometry-based tests, such as osmotic fragility, direct antiglobulin, and eosin 5-maleimide binding test, yielded results compatible with hereditary spherocytosis. Additionally, in family study, the results of eosin 5-maleimide binding test suggested his disease being hereditary. In cases with diagnostic difficulties, flow cytometry may be used as ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934753 Sat, 31 Jul 2010 23:00:00 +0100 3934753 http://www.medworm.com/index.php?rid=3934752&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805705%26dopt%3DAbstract CONCLUSIONS: The strong correlations and good clinical agreements of HbA(1c) between each analyzer expressed in terms of either NGSP or IFCC-derived NGSP indicate that these analyzers can be used interchangeably. PMID: 20805705 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934752 Sat, 31 Jul 2010 23:00:00 +0100 3934752 http://www.medworm.com/index.php?rid=3934751&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805706%26dopt%3DAbstract CONCLUSIONS: Urinary iodine analysis by ICP-MS showed good linearity, precision, accuracy, wide measuring range of detection, and lower LLOQ. This method will be very useful to evaluate the status of dietary iodine intake and the appropriateness of LID in thyroid cancer patients, thereby maximizing the effect of RAIT. PMID: 20805706 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934751 Sat, 31 Jul 2010 23:00:00 +0100 3934751 http://www.medworm.com/index.php?rid=3934750&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805707%26dopt%3DAbstract CONCLUSIONS: The diagnostic certainty (1-S) of whether to diagnose prostate cancer or not at a certain PSA level could be obtained using the information theory. The methodology used in this study may help interpret the results of other quantitative tests. PMID: 20805707 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934750 Sat, 31 Jul 2010 23:00:00 +0100 3934750 http://www.medworm.com/index.php?rid=3934749&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805708%26dopt%3DAbstract CONCLUSIONS: The species distribution and antifungal susceptibilities of yeasts may differ according to specimen type, testing method, hospital, and geographic region. Therefore, further large-scaled, long-term surveillance studies are needed to isolate yeasts and to confirm the species distribution and antifungal susceptibilities of yeast isolates from clinical specimens in Korea. PMID: 20805708 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934749 Sat, 31 Jul 2010 23:00:00 +0100 3934749 http://www.medworm.com/index.php?rid=3934748&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805709%26dopt%3DAbstract CONCLUSIONS: The panels compared favorably with conventional methods for the ID and AST of S. aureus. However, we expected a better performance for ID of E. faecium and AST using Synercid. PMID: 20805709 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934748 Sat, 31 Jul 2010 23:00:00 +0100 3934748 http://www.medworm.com/index.php?rid=3934747&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805710%26dopt%3DAbstract CONCLUSIONS: We observed that a combination of histology and PCR affords a high detection rate of H.pylori infection and that DPO-based PCR can be practically used for the diagnosis of H. pylori infection and the determination of clarithromycin resistance. These techniques were useful for biopsy sampling simultaneously from the antrum and body for the detection of clarithromycin resistance of multiple strain infection or heteroresistance. PMID: 20805710 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934747 Sat, 31 Jul 2010 23:00:00 +0100 3934747 http://www.medworm.com/index.php?rid=3934746&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805711%26dopt%3DAbstract CONCLUSIONS: The detection rate of H1N1 was significantly lower in immunocompromised patients than in other patients. The Ct value of patients with hematologic malignancy was significantly lower than that of other immunocompromised patients and control subjects. PMID: 20805711 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934746 Sat, 31 Jul 2010 23:00:00 +0100 3934746 http://www.medworm.com/index.php?rid=3934745&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805712%26dopt%3DAbstract CONCLUSIONS: Our study shows, for the first time, that BBC agar, with its good agreement and ease of use, is a valuable alternative to the API 50CH/B system for the presumptive identification of B. cereus isolates from blood cultures. PMID: 20805712 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934745 Sat, 31 Jul 2010 23:00:00 +0100 3934745 http://www.medworm.com/index.php?rid=3934744&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805713%26dopt%3DAbstract Authors: Seo JY, Ma YE, Lee JH, Lee ST, Ki CS, Lee NY Penicillium marneffei is the only dimorphic fungus among Penicillium spp. that can cause a fatal infection in immunocompromised patients. P. marneffei is endemic in Southeast Asia and eastern China. P. marneffei infection is an AIDS-defining illness and the third most common opportunistic infection in the endemic regions. Here, the authors report a case of disseminated P. marneffei infection in a patient who underwent liver transplantation in China. During the hospital stay, the mold form of the fungus that produced a red wine-colored pigment on the agar plate was isolated from the patient's urine, transtracheal aspirate, and blood. The fungus was identified as P. marneffei by direct sequencing of the D1-D2 and ITS regions. Thermal ... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934744 Sat, 31 Jul 2010 23:00:00 +0100 3934744 http://www.medworm.com/index.php?rid=3934743&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805714%26dopt%3DAbstract CONCLUSIONS: Serum PCT level was a better marker than CRP level or ESR for the diagnosis of lobar pneumonia in children with CAP. PMID: 20805714 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934743 Sat, 31 Jul 2010 23:00:00 +0100 3934743 http://www.medworm.com/index.php?rid=3934742&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805715%26dopt%3DAbstract CONCLUSIONS: Our results suggest that T-SPOT.TB test is very sensitive for diagnosing active tuberculosis. NTM may be the main cause of AFB culture positive and T-SPOT.TB negative results, but MTB infection in immunocompromised patients also has to be considered. PMID: 20805715 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934742 Sat, 31 Jul 2010 23:00:00 +0100 3934742 http://www.medworm.com/index.php?rid=3934741&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805716%26dopt%3DAbstract CONCLUSIONS: LG-Screen showed a favorable agreement with RIDA-Screen and ImmunoCAP assays, and it could be used for the detection of allergen specific IgE in the clinical laboratory. PMID: 20805716 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934741 Sat, 31 Jul 2010 23:00:00 +0100 3934741 http://www.medworm.com/index.php?rid=3934740&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805717%26dopt%3DAbstract CONCLUSIONS: The kit was effective for detecting microdeletions of the Yq. We identified microdeletions in 12% of the infertile patients. This Y chromosome microdeletion detection kit is useful for screening Yq microdeletions in infertile patients. PMID: 20805717 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934740 Sat, 31 Jul 2010 23:00:00 +0100 3934740 http://www.medworm.com/index.php?rid=3934739&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805718%26dopt%3DAbstract We describe a case of pseudoisodicentric chromosome 18q without mosaicism, which was confirmed from fetal cells in the amniotic fluid used for prenatal diagnosis of multiple congenital anomalies. A 23-yr-old pregnant woman was suspected of having a fetal anomaly at 18(+3) weeks gestation. In sonography, the fetus showed multiple anomalies: bilateral overt ventriculomegaly in the brain, ventricular septal defect and valve anomaly in the heart, bilateral club foot, polydactyly, meningocele, and a single umbilical artery. The pregnancy was terminated and a conventional G-banded chromosome study was performed using amniotic fluid. Twenty metaphase cells among the cultured amniocytes showed a 46,XX,psu idic(18)(q22). Consequently, the fetus had partial trisomy (18pter-->q22) and partial mono... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934739 Sat, 31 Jul 2010 23:00:00 +0100 3934739 http://www.medworm.com/index.php?rid=3934738&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20805719%26dopt%3DAbstract CONCLUSIONS: We proposed test panels based on the chief complaints of the patients; these test panels could allow rapid diagnosis and be more useful than the organ-specific or disease-specific tests in critical pathway development. The next step will be evaluating the efficiency and cost effectiveness of the test panel that we suggested. PMID: 20805719 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3934738 Sat, 31 Jul 2010 23:00:00 +0100 3934738 http://www.medworm.com/index.php?rid=3734364&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603578%26dopt%3DAbstract Authors: Lee KW, Park MH Human leukocyte antigen (HLA) gene region encodes a set of HLA molecules functioning critical roles in immune response. Each HLA gene locus shows extensive polymorphism with ever-increasing number of alleles. The HLA nomenclature system for alleles defined by DNA typing was first established in 1987 and has been revised several times. Recently, it has been revised again with a new frame that can accommodate ever-increasing number of new alleles. The new system has also introduced the novel suffixes, P and G, to simplify reporting of ambiguous strings of alleles in typing reports. This review introduces the HLA nomenclature system-2010 in conjunction with its clinical application in Koreans. PMID: 20603578 [PubMed - in process] (Source: The Korean Journal of... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734364 Mon, 31 May 2010 23:00:00 +0100 3734364 http://www.medworm.com/index.php?rid=3734363&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603579%26dopt%3DAbstract CONCLUSIONS: Although the association between inv(9) variation and CML is still controversial, we believe that hematologists should consider the role of constitutional inv(9) variation in CML patients to avoid overlooking the impaired engraftment potential of hematopoietic stem cells harboring inv(9). Therefore, we suggest that more effort should be invested to develop cytogenetic tests for detecting constitutional inv(9) variation in CML patients. PMID: 20603579 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734363 Mon, 31 May 2010 23:00:00 +0100 3734363 http://www.medworm.com/index.php?rid=3734362&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603580%26dopt%3DAbstract CONCLUSIONS: The myelocrit is a simple, reproducible and objective method to determine the BM cellularity. For accurate assessment of BM cellularity, measurement of the thickness of BC layer in AML D7 BM and of the myelocrit cellularity in other BM samples has better be used. PMID: 20603580 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734362 Mon, 31 May 2010 23:00:00 +0100 3734362 http://www.medworm.com/index.php?rid=3734361&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603581%26dopt%3DAbstract CONCLUSIONS: AML MRC patients showed a lower CR rate and shorter OS and EFS than AML NOS patients. AML MRC patients with MLD showed similar results and their prognosis was not different from those without MLD. MLD findings among AML MRC could be an independent poor prognostic factor in de novo AML. PMID: 20603581 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734361 Mon, 31 May 2010 23:00:00 +0100 3734361 http://www.medworm.com/index.php?rid=3734360&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603582%26dopt%3DAbstract In this study, we have reported presumably the first case of this chromosomal abnormality in Korea, thereby facilitating the delineation of a distinct subtype of ALL. A 57-yr-old woman was referred to our hospital because of pancytopenia. Peripheral blood examination showed 55% blasts. The bone marrow was markedly hypercellular, and about 82.4% of all nucleated cells were blasts. The results of immunophenotyping and cytochemical staining suggested early precursor B-ALL. Cytogenetic analysis of the bone marrow cells showed a complex karyotype, including a reciprocal translocation between the short arm of chromosome 12 and the long arm of chromosome 17, t(12;17)(p13;q21). Data from array comparative genomic hybridization were almost consistent with the cytogenetic findings. PMID: 2060358... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734360 Mon, 31 May 2010 23:00:00 +0100 3734360 http://www.medworm.com/index.php?rid=3734359&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603583%26dopt%3DAbstract We report a case of thymoma-complicated pure red cell aplasia and amegakaryocytic thrombocytopenia in a 73-yr-old woman. Pure red cell aplasia was diagnosed seven months after the detection of thymoma. One year after the diagnosis of pure red cell aplasia and seven months after thymectomy, bone marrow aspiration and biopsy showed an absence of megakaryocytes, marked erythroid hypoplasia with normal myeloid series. A diagnosis of amegakaryocytic thrombocytopenia and pure red cell aplasia was made. Oral steroid maintenance therapy resulted in recovery of platelet count. She has still transfusion-dependant anemia but platelet and neutrophil counts had been maintained in normal range for more than five months, until the last follow-up. We think that autoreactive T cells may induce a clinical a... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734359 Mon, 31 May 2010 23:00:00 +0100 3734359 http://www.medworm.com/index.php?rid=3734358&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603584%26dopt%3DAbstract We describe a case of 3-month-old infant who presented with severe anemia, elevated levels of HbF and adenosine deaminase and bilateral hydronephrosis, who was later confirmed as DBA by mutation analysis using the direct sequencing method. Direct sequencing analysis of RPS19 gene was performed with both cDNA and genomic DNA extracted from peripheral blood and a c.3G>A point mutation of exon 2 resulting in p.Met1Ile was identified in this patient. The patient showed an inadequate response to steroid therapy and a partial response to RBC transfusion with a follow-up Hb level of 8.3 g/dL on her last visit to the outpatient clinic. DBA is a genetically and phenotypically heterogeneous disease, and we have reviewed the clinical characteristics of 25 Korean patients thus far reported in the l... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734358 Mon, 31 May 2010 23:00:00 +0100 3734358 http://www.medworm.com/index.php?rid=3734357&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603585%26dopt%3DAbstract We report a case of therapy-related ALL (t-ALL) with MLL gene rearrangement in a patient who had undergone treatment for breast cancer. A 60-yr-old woman with breast cancer underwent breast-conserving surgery followed by 6 cycles of adjuvant chemotherapy (cyclophosphamide, epirubicin, and fluorouracil) and radiation therapy (dose, 5,040 cGy to the left breast and a 1,000 cGy boost to the tumor bed). A follow-up examination performed 14 months after the chemotherapy revealed no evidence of breast malignancy. However, the patient's complete blood cell count indicated acute leukemia: white blood cell count, 174.1x10(9)/L with 88% blasts; Hb level, 12.5 g/dL; and platelet count, 103.0x10(9)/L. Examination of the bone marrow aspirate smear revealed a high percentage of blasts (85.1% of all nucl... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734357 Mon, 31 May 2010 23:00:00 +0100 3734357 http://www.medworm.com/index.php?rid=3734356&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603586%26dopt%3DAbstract In this study, we used the Bethesda assay to determine the incidence of FVIII inhibitors in 75 fresh plasma samples obtained from 50 hemophilia A patients, and then used ELISA and the Bethesda assay to determine the titres of these inhibitors after the samples had been frozen and thawed. The samples from the screening Bethesda assay were centrifuged and stored at -70 degrees C in accordance with the assay guidelines. Subsequently, these samples were thawed and analyzed using ELISA and the Bethesda assay. The incidence of inhibitors in hemophilia A patients was 20.0%. Among the 35 inhibitor-positive samples identified in the screening Bethesda assay, 16 were positive in ELISA while only 4 were positive in the repeated Bethesda assay. In this study, the ELISA technique showed a higher sensit... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734356 Mon, 31 May 2010 23:00:00 +0100 3734356 http://www.medworm.com/index.php?rid=3734355&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603587%26dopt%3DAbstract CONCLUSIONS: BGT at the forearm was highly consistent with the standard BGT, thereby confirming its applicability in clinical practice for self-testing under steady fasting conditions. PMID: 20603587 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734355 Mon, 31 May 2010 23:00:00 +0100 3734355 http://www.medworm.com/index.php?rid=3734354&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603588%26dopt%3DAbstract CONCLUSIONS: TTP can be used to discriminate pathogens and contaminants. The shorter TTP in anaerobic vials of certain Enterobacteriaceae and Enterococcus spp. would facilitate further identification. DTP is useful for diagnosing catheter-related bloodstream infection by S. aureus, S. epidermidis, and Candida spp. PMID: 20603588 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734354 Mon, 31 May 2010 23:00:00 +0100 3734354 http://www.medworm.com/index.php?rid=3734353&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603589%26dopt%3DAbstract Authors: Hwang S, Kim J, Yoon S, Cha Y, Kim M, Yong D, Chang JH, Jeong SH, Uh Y, Lee K A yeast-like strain was isolated from the brain abscess of a patient diagnosed with astrocytoma. Morphological and molecular analysis on D1/D2 domain in the 26S rRNA gene and internal transcript spacer region of the strain revealed that the strain belonged to the genus Pseudozyma. To the best of our knowledge, this is the first report on the isolation of a Pseudozyma strain from brain abscess. PMID: 20603589 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734353 Mon, 31 May 2010 23:00:00 +0100 3734353 http://www.medworm.com/index.php?rid=3734352&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603590%26dopt%3DAbstract CONCLUSIONS: Multiplex RT-PCR provided faster results and had higher detection rates than R-mix virus culture. Further, unlike R-mix virus culture, multiplex RT-PCR can be used to identify new respiratory viruses. Therefore, multiplex RT-PCR is more useful than R-mix virus culture in the diagnosis of respiratory virus infection. PMID: 20603590 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734352 Mon, 31 May 2010 23:00:00 +0100 3734352 http://www.medworm.com/index.php?rid=3734351&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603591%26dopt%3DAbstract CONCLUSIONS: In Korea, the resistance of S. maltophilia isolates to TMP/SMX is due to sul1 within a class 1 integron rather than to sul2. The class 1 integron also harbors multiple antibiotic resistance genes in addition to sul1, and therefore it could mediate multidrug resistance in S. maltophilia. PMID: 20603591 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734351 Mon, 31 May 2010 23:00:00 +0100 3734351 http://www.medworm.com/index.php?rid=3734350&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603592%26dopt%3DAbstract CONCLUSIONS: Unlike TNFalpha, IL-6 reflects disease activity of RA and shows a better agreement with treatment response than ESR or CRP, indicating that it has an association with clinical features of RA. Therefore IL-6 could be used as an additional marker in the evaluation of treatment response when markers like ESR or CRP show results discordant from clinical features. PMID: 20603592 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734350 Mon, 31 May 2010 23:00:00 +0100 3734350 http://www.medworm.com/index.php?rid=3734349&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603593%26dopt%3DAbstract CONCLUSIONS: The 2 Korean patients with bisalbuminemia showed genetic variations, including a novel missense mutation. The ALB gene analysis with 3D modeling is useful for determining the nature of bisalbuminemia and for predicting the effects on the albumin-binding affinity of other biochemical compounds. PMID: 20603593 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734349 Mon, 31 May 2010 23:00:00 +0100 3734349 http://www.medworm.com/index.php?rid=3734348&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603594%26dopt%3DAbstract Authors: Lee JH, Cho HS, Lee ES, Jung BC Partial trisomy 2p is a rare but relatively well-defined syndrome with distinctive clinical features, including marked psychomotor delay, dysmorphic face, and congenital heart disease. The phenotype of trisomy 18p is variable, from normal appearance to moderate mental retardation. Most cases of trisomy 2p and trisomy 18p result from the inheritance of an unbalanced segregant from a balanced parental translocation or due to de novo duplication. Here, we present the first report of a combined partial trisomy 2p and trisomy 18p due to a supernumerary marker chromosome (SMC). The final karyotype of the patient was 47,XX,+der(18)t(2;18)(p23.1;q11.1)[22]/46,XX[8]. The patient had typical dysmorphic features of partial trisomy 2p23-pter syndrome and co... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734348 Mon, 31 May 2010 23:00:00 +0100 3734348 http://www.medworm.com/index.php?rid=3734347&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603595%26dopt%3DAbstract We present the case of a 13-yr-old girl with the main clinical features of 15q overgrowth syndrome and a 46,XX,dup(15)(q24q26.3)[117]/46,XX[3].ish dup(15)(q24q26.3) (SNPRN+,PML+,subtel++,tel++) de novo karyotype. The findings in this case are consistent with those in the previous distal 15q trisomy cases that presented with overgrowth and mental retardation. Further, the rearranged chromosome had a double set of directly oriented telomeric and subtelomeric sequences. PMID: 20603595 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734347 Mon, 31 May 2010 23:00:00 +0100 3734347 http://www.medworm.com/index.php?rid=3734346&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20603596%26dopt%3DAbstract Authors: Park CH, Lee ST, Ki CS, Kim JW The human apolipoprotein E (APOE) gene contains several single-nucleotide polymorphisms (SNPs) that are distributed across the gene. The genotype of the APOE gene has important implications as a risk factor for various diseases. We observed 2 cases in which the results of allele-specific PCR (AS-PCR) of the APOE gene were not consistent with those of fluorescence resonance energy transfer (FRET) or sequencing analysis. In these cases, genotyping by AS-PCR showed that patients were epsilon2 homozygotes, while sequencing analysis and FRET showed that they were epsilon2/epsilon3 heterozygotes. Herein, we describe the causes of the errors in genotyping and describe the significance of these errors. PMID: 20603596 [PubMed - in process] (Source: Th... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3734346 Mon, 31 May 2010 23:00:00 +0100 3734346 http://www.medworm.com/index.php?rid=3546061&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445324%26dopt%3DAbstract CONCLUSIONS: Gating the granulocytic region is a relatively easy method for MDS immunophenotyping. Among the parameters studied, SSC, CD10, and CD56 were the most useful for differentiating MDS from non-clonal disorders. While immunophenotypic changes in MDS appear to be useful for differentiating MDS from non-clonal disorders, these changes were also noted in the mature cells of leukemic patients. PMID: 20445324 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546061 Wed, 31 Mar 2010 23:00:00 +0100 3546061 http://www.medworm.com/index.php?rid=3546060&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445325%26dopt%3DAbstract CONCLUSIONS: In patients with suspected ITP, cytogenetic analysis should be done. If specific clonal chromosomal abnormality is found, presumptive diagnosis of MDS has to be considered and close follow up is needed. PMID: 20445325 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546060 Wed, 31 Mar 2010 23:00:00 +0100 3546060 http://www.medworm.com/index.php?rid=3546059&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445326%26dopt%3DAbstract Authors: Park J, Kim M, Lim J, Kim Y, Cho B, Park YJ, Han K Severe congenital neutropenia is a rare hematological disease characterized by a selective decrease in circulating neutrophils, maturation arrest of granulocytic precursors at the promyelocyte stage, and recurrence of infections. A 2-month-old male infant (patient A) and a 14-month-old female child (patient B) were referred to our hospital due to severe neutropenia. Sequencing analysis of ELA2 and HAX1 genes was performed. Two single nucleotide polymorphisms of HAX1 gene were found. They were 5,104T-->G point mutation of exon 1 and 5,474A-->G point mutation of intron 1 in HAX1 gene. The mutation of ELA2 gene was not found. The patient A showed a good response to granulocyte colony-stimulating factor (G-CSF) treatment and... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546059 Wed, 31 Mar 2010 23:00:00 +0100 3546059 http://www.medworm.com/index.php?rid=3546058&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445327%26dopt%3DAbstract Authors: Huh JY, Chung S, Oh D, Kang MS, Eom HS, Cho EH, Han MH, Kong SY The translocation t(10;11)(p13;q14q21) has been found to be recurrent in acute lymphoblastic and myeloid leukemias, and results in the fusion of the clathrin assembly lymphoid myeloid leukemia (CALM) gene with the AF10 gene; these genes are present on chromosomes 11 and 10, respectively. Because the CALM-AF10 rearrangement is a rare chromosomal abnormality, it is not included in routine molecular tests for acute leukemia. Here, we describe the cases of 2 patients with the CALM-AF10 fusion gene. The first patient (case 1) was diagnosed with T-cell ALL, and the second patient (case 2) was diagnosed with AML. Both patient samples showed expression of the homeobox A gene cluster and the histone methyltransferase hDOT1... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546058 Wed, 31 Mar 2010 23:00:00 +0100 3546058 http://www.medworm.com/index.php?rid=3546057&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445328%26dopt%3DAbstract Authors: Lee KK, Cho HI, Chi HS, Kim do Y, Chae SL, Huh HJ Essential thrombocythemia (ET) is a chronic myeloproliferative neoplasm that involves primarily the megakaryocytic lineage. After many years, a few patients with ET may develop bone marrow (BM) fibrosis and rarely develop osteosclerosis. A 60-yr-old female was admitted due to severe left upper quadrant abdominal discomfort. She had been diagnosed as ET 19 yrs ago. On liver computed tomography severe splenomegaly was shown. Laboratory tests revealed WBC 24.3x10(9)/L, hemoglobin 13.4 g/dL, platelets 432x10(9)/L, lactate dehydrogenase 4,065 IU/L (reference range; 240-480). Blood smear demonstrated leukoerythroblastosis, teardrop cells, and giant and hypogranular platelets. BM study revealed inadequate aspirate due to dry tap. BM b... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546057 Wed, 31 Mar 2010 23:00:00 +0100 3546057 http://www.medworm.com/index.php?rid=3546056&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445329%26dopt%3DAbstract CONCLUSIONS: We established gestational age-specific second-trimester median values for four markers in Korean samples using the UniCel DxI 800 immunoassay system. Despite significant bias, there were good correlations between the results obtained using the UniCel DxI 800 immunoassay and those obtained using the Immulite 2000 assay. PMID: 20445329 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546056 Wed, 31 Mar 2010 23:00:00 +0100 3546056 http://www.medworm.com/index.php?rid=3546055&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445330%26dopt%3DAbstract CONCLUSIONS: The Quick anaero-system is a simple and effective method of culturing obligate anaerobes, and its performance is superior to that of the BD GasPak EZ Anaerobe Container System. PMID: 20445330 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546055 Wed, 31 Mar 2010 23:00:00 +0100 3546055 http://www.medworm.com/index.php?rid=3546054&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445331%26dopt%3DAbstract CONCLUSIONS: VRE genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using multiplex real-time PCR assay and melting curve analysis. PMID: 20445331 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546054 Wed, 31 Mar 2010 23:00:00 +0100 3546054 http://www.medworm.com/index.php?rid=3546053&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445332%26dopt%3DAbstract CONCLUSIONS: The rapid antigen test is significantly less sensitive than PCR assay; therefore, it is not useful for H1N1 detection; however multiplex PCR, the AdvanSure real-time PCR, and the Accu-Power real-time PCR can be useful for H1N1 detection. PMID: 20445332 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546053 Wed, 31 Mar 2010 23:00:00 +0100 3546053 http://www.medworm.com/index.php?rid=3546052&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445333%26dopt%3DAbstract CONCLUSIONS: We suggest that, compared with CRP, PCT is a better diagnostic and discriminative biomarker of sepsis categorized according to the ACCP/SCCM. Moreover, catheter-associated bacteremia could be discriminated from sepsis using PCT concentration. PMID: 20445333 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546052 Wed, 31 Mar 2010 23:00:00 +0100 3546052 http://www.medworm.com/index.php?rid=3546051&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445334%26dopt%3DAbstract We report a case of infective endocarditis with colon cancer caused by S. gallolyticus subsp. gallolyticus (previously named S. bovis biotype I). A 59-yr-old woman presented with a 1-month history of fever. Initial blood cultures were positive for gram-positive cocci, and echocardiography showed vegetation on mitral and aortic valves. Antibiotic treatment for infective endocarditis was started. The infecting strain was a catalase-negative and bile-esculin-positive alpha-hemolytic Streptococcus susceptible to penicillin and vancomycin. The strain was identified as S. gallolyticus subsp. gallolyticus with the use of the Vitek 2 GPI and API 20 Strep systems (bioMérieux, USA). The 16S rDNA sequences of the blood culture isolates showed 100% homology with those of S. gallolyticus subsp. gall... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546051 Wed, 31 Mar 2010 23:00:00 +0100 3546051 http://www.medworm.com/index.php?rid=3546050&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445335%26dopt%3DAbstract Authors: Chung JW, Cha YJ, Oh DJ, Nam WJ, Kim SH, Lee MK, Kim HR Isolated bone marrow infection by nontuberculous mycobacteria (NTM) is extremely rare. Recently, we encountered a case of bone marrow Mycobacterium avium complex (MAC) infection, which presented as a fever of unknown origin shortly after starting continuous ambulatory peritoneal dialysis (CAPD). The patient was diagnosed with MAC infection on the basis of PCR-restriction fragment length polymorphism analysis and sequencing of DNA obtained from bone marrow specimens. Although this was a case of severe MAC infection, there was no evidence of infection of other organs. End-stage renal disease (ESRD) patients undergoing dialysis can be considered immunodeficient; therefore, when these patients present with fever of unknown or... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546050 Wed, 31 Mar 2010 23:00:00 +0100 3546050 http://www.medworm.com/index.php?rid=3546049&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445336%26dopt%3DAbstract CONCLUSIONS: T-SPOT.TB test would be a very useful screening and supplementary test for diagnosis of tuberculosis due to its high sensitivity. However, positive results of T-SPOT.TB should be cautiously interpreted because of high positivity in treated tuberculosis patients and healthy volunteers in Korea. PMID: 20445336 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546049 Wed, 31 Mar 2010 23:00:00 +0100 3546049 http://www.medworm.com/index.php?rid=3546048&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445337%26dopt%3DAbstract CONCLUSIONS: Although ICAs were more sensitive than RPHAs, they had variable sensitivities for HBsAg and were less sensitive than the automated immunoassay kits. Therefore, ICAs and RPHAs should be used with caution in the screening tests for HBsAg and their sensitivities need to be improved. PMID: 20445337 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine) The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546048 Wed, 31 Mar 2010 23:00:00 +0100 3546048 http://www.medworm.com/index.php?rid=3546047&cid=s_36967_166_f&fid=36967&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20445338%26dopt%3DAbstract In this study, we assessed 45 patients for whom a vitamin B12 test had been requested and 139 anemic patients. We investigated the associations between the levels of homocysteine (Hcy) and those of holoTC, serum vitamin B12, and folate and assessed the diagnostic value of holoTC levels as a marker for vitamin B12 deficiency. We also determined the precision of the AxSYM holoTC assay by calculating the coefficient of variance (CV). The within-run and between-run precision values were excellent, as all CV values were less than 3.5%. The holoTC levels were low (<35 pmol/L) in 7 samples, and 6 of these samples had normal total serum vitamin B12 levels. In 2 of these samples, high Hcy levels (>12 micromol/L) indicated vitamin B12 deficiency. Thus, the holoTC levels were more sensitive tha... The Korean Journal of Laboratory Medicine journals http://www.medworm.com/rss/comments.php?id=3546047 Wed, 31 Mar 2010 23:00:00 +0100 3546047