MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Tissue Antigens' source. Mon, 22 Mar 2010 13:40:56 +0100 http://www.medworm.com/index.php?rid=3364275&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01472.x HLA-A*240220 has one nucleotide change from HLA-A*24020101 at position 201 in exon 2 from G to C. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364275 Mon, 15 Mar 2010 00:00:00 +0100 3364275 http://www.medworm.com/index.php?rid=3364281&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01471.x Cytokines play important roles in the pathogenesis of lipodystrophy syndrome (LS). Single nucleotide polymorphisms (SNPs) at positions [minus]607(C/A) and [minus]137(C/G) in the promoter region of the interleukin-18 (IL-18) gene and at position +874(T/A) of the interferon-gamma (IFN-[gamma]) gene are related to the expression of these cytokines. To examine whether IL-18 and IFN-[gamma] polymorphisms are associated with LS, these SNPs were genotyped in 88 human immunodeficiency virus (HIV)-infected patients presenting LS, 79 HIV-infected without LS, and 133 healthy controls. The [minus]607A allele, [minus]607AA genotype, and [minus]137G/[ndash]607A and [minus]137C/[ndash]607A haplotypes in the IL-18 gene were over-represented in HIV patients presenting LS. The [minus]137G/[minus]607C haplot... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364281 Sun, 14 Mar 2010 00:00:00 +0100 3364281 http://www.medworm.com/index.php?rid=3364280&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01463.x Demodectic mange is a well-known parasitic skin disease characterized by the presence of a larger than normal number of Demodex mites (Demodex canis) in the skin of dogs. Recent research has suggested that major histocompatibility complex (MHC) class II expression is higher in the skin of dogs suffering from demodicosis than in normal ones. We have investigated whether canine Dog Leukocyte Antigen (DLA) class II alleles are associated with canine juvenile generalized demodicosis (JGD). In the present study, the analysis of microsatellite markers (FH2202, FH2975 and FH2054) linked to DLA was made in Boxer, Argentinean Mastiff and mixed breed dogs. DNA samples from 56 dogs affected with the disease and 60 breed-matched controls collected in Argentina were analysed. A highly significant assoc... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364280 Sun, 14 Mar 2010 00:00:00 +0100 3364280 http://www.medworm.com/index.php?rid=3364279&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01474.x Here, we describe the identification of a novel human leukocyte antigen (HLA)-DRB1 allele, DRB1*1189, that was found in an Italian Caucasian individual. This sequence differs from HLA-DRB1*1134 by three nucleotide exchange at positions 286 (C[rarr]T), 296 (A[rarr]G), and 308 (C[rarr]A) in exon 2. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364279 Sun, 14 Mar 2010 00:00:00 +0100 3364279 http://www.medworm.com/index.php?rid=3364278&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01475.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364278 Sun, 14 Mar 2010 00:00:00 +0100 3364278 http://www.medworm.com/index.php?rid=3364277&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01459.x The killer immunoglobulin-like receptor (KIR) anthropology component of the 15th International Histocompatibility Workshop (IHIWS) sought to explore worldwide population variation in the KIR loci, and to examine the relationship between KIR genes and their human leukocyte antigen (HLA) ligands. Fifteen laboratories submitted KIR genotype and HLA ligand data in 27 populations from six broad ethnic groups. Data were analyzed for correlations between the frequencies of KIR and their known HLA ligands. In addition, allelic typing was performed for KIR2DL2 and 3DL1 in a subset of populations. Strong and significant correlations were observed between KIR2DL2, 2DL3 genotype frequencies and the frequency of their ligand, HLA-C1. In contrast, only weak associations were seen for 3DL1, 3DS1 and the ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364277 Sun, 14 Mar 2010 00:00:00 +0100 3364277 http://www.medworm.com/index.php?rid=3364276&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01473.x HLA-B*550205 shows one nucleotide difference from HLA-B*550201 at position 756 in exon 4 from T to C. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3364276 Sun, 14 Mar 2010 00:00:00 +0100 3364276 http://www.medworm.com/index.php?rid=3355383&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01458.x The novel HLA-DQB1*0636 allele differs from HLA-DQB1*060401 in one nucleotide substitution at codon 186 in exon 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3355383 Fri, 12 Mar 2010 00:00:00 +0100 3355383 http://www.medworm.com/index.php?rid=3355386&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01447.x It was recently shown that the CD24 Ala57Val (rs 52812045) polymorphism plays a significant role in susceptibility to systemic lupus erythematosus (SLE) in a Spanish population, which has not been confirmed in other ethnic groups. We investigated the distribution of the CD24 Ala57Val polymorphism in patients with SLE (n = 250) and controls (n = 350) in Poland. The odds ratio (OR) for patients with SLE with the Ala/Val genotype compared with Ala/Ala genotype was 1.490 [95% confidence interval (CI) = 1.052[ndash]2.111, P = 0.0275], and OR for the Val/Val genotype compared with Ala/Ala genotypes was 2.001 (95% CI = 1.154[ndash]3.467, P = 0.0154). Moreover, we observed a significant association between the CD24 Val allele and the presence of anti-Scl-70 antibody (Ab) OR = 2.155 (1.438[ndash]3.... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3355386 Thu, 11 Mar 2010 00:00:00 +0100 3355386 http://www.medworm.com/index.php?rid=3355385&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01448.x Allele frequencies (AFs) and haplotypic associations of human leukocyte antigen (HLA) class I and II were investigated in 400 unrelated, healthy, ethnic Northeast Thais. HLA-A, -B, -Cw, -DRB1 and -DQB1 were typed by polymerase chain reaction-sequence specific primer, -sequence specific oligonucleotide probe and -single-strand conformation polymorphism methods. In this population, 17 HLA-A, 26 HLA-B, 15 HLA-Cw, 26 HLA-DRB1 and 13 HLA-DQB1 alleles (or groups of alleles) were found. AFs > 10% included A*11 (23.3%), 24 (18.8%), 0207 (14.4%), 33 (11.5%), 0203 (10.6%); B*4601 (13.9%); Cw*07(01-03) (18.5%), 01 (15.9%), 04 (12.0%), 0304 (10.6%); DRB1*1502 (18.5%), 1202 (13.4%); DQB1*0502 (20.3%), 0501 (16.3%), 0301 (14.1%) and 02 (10.9%). The most common of 2-locus haplotypes included A*0207-B*460... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3355385 Thu, 11 Mar 2010 00:00:00 +0100 3355385 http://www.medworm.com/index.php?rid=3355384&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01450.x This study aimed to report antigen and haplotype frequencies (HFs) in the Thai Stem Cell Donor Registry (TSCDR). From 16,807 human leukocyte antigen (HLA)-A, -B, and -DR typed donors, 19 HLA-A, 41 HLA-B, and 13 HLA-DR antigens were found. HLA-A43, A80, B78, B82, B83, and DR18 were not observed. A total of 1921 haplotypes were estimated and 245 haplotypes were reliable and their cumulative HF was 75.74%. Similar to previous Thai subpopulation studies, the most common haplotypes were HLA-A33-B58-DR17 (4.54%), A2-B46-DR9 (4.11%), A33-B44-DR7 (2.85%), and A11-B75-DR12 (2.00%). To evaluate the probability in finding matched donors, from April 2002 to August 2008, 793 Thai patients were requested for unrelated stem cell donor searching. The number of patients with HLA-A, -B, -DR split matched do... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3355384 Thu, 11 Mar 2010 00:00:00 +0100 3355384 http://www.medworm.com/index.php?rid=3351349&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01468.x KIR3DL1 and KIR3DS1 allele frequencies were determined by DNA sequencing of the complete coding regions from 100 random unrelated African Americans. Alleles 3DL1*01501 (29 individuals) and 3DL1*01502 (23 individuals) were most frequently observed in addition to 21 other known alleles and 7 new alleles: KIR3DL1*01503, *03102, *064, *065, *066, *067, and *068. Two of the new alleles (KIR3DL1*064, *065) created additional variants of the chimeric KIR3DL1/KIR3DL2 gene. KIR3DS1*01301 (94% of the gene positive individuals) and KIR3DS1*049N (11%) were identified in the 18 individuals carrying this gene. Three individuals appeared to carry a killer cell immunoglobulin-like receptor haplotype with KIR3DL1/S1 duplication. The profile of alleles resembles that found in African populations but also sh... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3351349 Thu, 11 Mar 2010 00:00:00 +0100 3351349 http://www.medworm.com/index.php?rid=3351350&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01476.x The major histocompatibility complex (MHC) class II transactivator (MHC2TA) is known as a master regulator for expression of MHC class II molecules. In the present study, we investigated the influence on the risk for sarcoidosis of two variants of the MHC2TA gene, selected from previous association studies of inflammatory diseases. Seven hundred and twenty-eight sarcoidosis patients and 873 controls matched by ethnicity were included in the study. Patients were classified as with Löfgren's syndrome (or not) as subphenotypes. Individuals were genotyped for two single nucleotide polymorphisms (SNPs) of the MHC2TA gene, rs3087456 A/G and rs11074932 C/T, and were human leukocyte antigen (HLA)-DRB1-typed. After correction for multiple testing, our data showed a significant association with Lö... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3351350 Wed, 10 Mar 2010 00:00:00 +0100 3351350 http://www.medworm.com/index.php?rid=3332304&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01461.x Human leukocyte antigen (HLA)-DRB1*1454 differs from HLA-DRB1*1401 in only one position in exon 3. Before description of this polymorphism both alleles were routinely typed as HLA-DRB1*1401. This prompted our study on relative frequency of these alleles. We determined relative frequencies of DRB1*1454 and DRB1*1401 by sequence-based tying (SBT) in 106 samples and found DRB1*1454 in 87.9% and DRB1*1401 in 12.1% of previously DRB1*1401/54 ambiguous tested individuals. Population frequencies were estimated using data from the local bone marrow donor database. Phenotype and genotype frequency of DRB1*1454 and DRB1*1401 were 5.592%, 0.2828, and 0.773%, 0.00391, respectively. The corresponding figures for DRB1*1404 were 0.238% and 0.00119. Other DRB1*14 alleles were very rare. The most frequent ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3332304 Fri, 05 Mar 2010 00:00:00 +0100 3332304 http://www.medworm.com/index.php?rid=3332307&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01464.x We report a novel KIR3DL1*072 allele that was found using a sequence-based typing approach. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3332307 Thu, 04 Mar 2010 00:00:00 +0100 3332307 http://www.medworm.com/index.php?rid=3332306&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01449.x Dogs represent an excellent comparative model for autoimmune thyroiditis as several dog breeds develop canine lymphocytic thyroiditis (CLT), which is clinically similar to Hashimoto's thyroiditis in human. We obtained evidence that dog leukocyte antigen (DLA) class II genotype function as either genetic risk factor that predisposes for CLT or as protective factor against the disease. Genetic diversity at their DLA-DRB1, -DQA1, and -DQB1 loci were defined and potential association to major histocompatibility complex II haplotypes and alleles was analyzed. Giant Schnauzers carrying the DLA-DRB1*01201/DQA1*00101/DQB1*00201 haplotype showed an increased risk (odds ratio of 6.5) for developing CLT. The same risk haplotype has, to date, been observed in three different breeds affected by this di... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3332306 Thu, 04 Mar 2010 00:00:00 +0100 3332306 http://www.medworm.com/index.php?rid=3332305&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01451.x This aim of the study was to investigate whether human leukocyte antigen (HLA)-DQA1*0505 sharing or the maternal killer immunoglobulin-like receptor (KIR) repertoire is associated with recurrent spontaneous abortion (RSA) or repeated implantation failure (RIF). The study included 224 couples with RSA, 61 couples with RIF, 182 fertile couples, and 10 couples with successful in vitro fertilization and embryo transfer (IVF)/ET at first cycle. HLA-DQA1*0505 typing using polymerase chain reaction-sequence-specific oligonucleotide (PCR-SSO) was performed in 185 RSA (117 with alloimmune abnormalities and 68 of autoimmune etiology), 61 RIF and 182 control couples, and KIR genotyping using polymerase chain reaction-sequence-specific primer (PCR-SSP) in 167 RSA and 55 RIF cases as well as 46 RSA and... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3332305 Thu, 04 Mar 2010 00:00:00 +0100 3332305 http://www.medworm.com/index.php?rid=3319086&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01460.x KIR2DL1 alleles were identified by DNA sequencing of the coding region from amplified genomic DNA from 100 random African Americans. The majority of individuals (97%) carried a KIR2DL1 locus. Allele KIR2DL1*00302 was found in 68% of individuals but KIR2DL1*00401, *002, *00303, *006, and *007 were also frequent. Eleven new alleles were described: KIR2DL1*00403, *01101, *01102, *012, *013N, *014, *015, *016, *017, *018, and *019. Nine of the novel alleles encoded amino acid substitutions located throughout the receptor; one allele carried a stop codon in the exon encoding the first extracellular domain. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3319086 Tue, 02 Mar 2010 00:00:00 +0100 3319086 http://www.medworm.com/index.php?rid=3303488&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01446.x This study represents the first report on the distribution of KIR genes in 205 unrelated healthy mestizo Venezuelan individuals. Genotyping analysis showed that all KIR genes are present in this population. Frequency of inhibitory killer cell immunoglobulin-like receptors (KIRs) exceeded 0.69, except for KIR2DL2 (0.29) and 2DL5 (0.37). Activating KIRs showed low frequencies (0.11[ndash]0.29), except for KIR2DS4 (0.68). Forty-five different KIR genotypes were identified, with a predominance of three genotypes found in 50.7% of the population of which 25.9% were individuals homozygous for haplotype A. The frequencies of KIR genes reflect the ethnic admixture existing in the mestizo Venezuelan population. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3303488 Thu, 25 Feb 2010 00:00:00 +0100 3303488 http://www.medworm.com/index.php?rid=3260074&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01462.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3260074 Thu, 11 Feb 2010 00:00:00 +0100 3260074 http://www.medworm.com/index.php?rid=3251728&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01456.x The new human leukocyte antigen (HLA) class I allele, HLA-Cw*0134, was identified in a Chinese individual. HLA-Cw*0134 differs from HLA-Cw*0124 by one nonsynonymous nucleotide change at the codon 99 (TGT to TAT) and one synonymous nucleotide change at the codon 127 (AAC to AAT). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3251728 Tue, 09 Feb 2010 00:00:00 +0100 3251728 http://www.medworm.com/index.php?rid=3251727&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01429.x This study is the first to report on HPA allele frequencies in Algerian populations. High allele frequencies were observed for HPA-1b (0.209[ndash]0.112), HPA-3b (0.411[ndash]0.312) and HPA-5b (0.217[ndash]0.087), leading to a high risk of alloimmunization in this population, especially the Mzab and the population of Annaba. Our results could have some impact in the diagnosis, prevention and treatment of alloimmune thrombocytopenia. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3251727 Tue, 09 Feb 2010 00:00:00 +0100 3251727 http://www.medworm.com/index.php?rid=3251726&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01455.x Recombination between HLA-G*01010101/02 and HLA-G*01010201 generates HLA-G*010120. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3251726 Tue, 09 Feb 2010 00:00:00 +0100 3251726 http://www.medworm.com/index.php?rid=3240704&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01457.x The novel allele HLA-A*030114 differs from HLA-A*03010101 by a silent nucleotide substitution at codon 153A (GCG to GCA). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3240704 Fri, 05 Feb 2010 00:00:00 +0100 3240704 http://www.medworm.com/index.php?rid=3225326&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01443.x CD1 molecules are the major histocompatibility complex (MHC)-like glycoproteins specialized in capturing and presenting a variety of glycolipid to antigen-specific T-cells. There are five closely linked CD1 genes termed as CD1a, CD1b, CD1c, CD1d, and CD1e. CD1 gene features limited the polymorphism in exon 2 which encodes for the [alpha]1 domain. Few investigations on the allele frequencies of the CD1 genes have been reported to date; however, variation of CD1 allele frequency in different ethnics has been observed. In the current study, the CD1a, CD1d, and CD1e gene polymorphisms in exon 2 (alleles 01 and 02) in a group of normal Chinese Han and She individuals were analyzed. Similar allele prevalence was observed between the two populations. The CD1e allele frequency was 37.1% (allele 01... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3225326 Mon, 01 Feb 2010 00:00:00 +0100 3225326 http://www.medworm.com/index.php?rid=3217537&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01453.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217537 Fri, 29 Jan 2010 00:00:00 +0100 3217537 http://www.medworm.com/index.php?rid=3217542&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01432.x In conclusion, our data suggest that the variation in class II HLA alleles could be a genetic factor involved in the susceptibility to primary DCM in the Tunisian population. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217542 Thu, 28 Jan 2010 00:00:00 +0100 3217542 http://www.medworm.com/index.php?rid=3217541&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01440.x Canine hypoadrenocorticism (Addison's disease) is due to a deficiency of corticosteroids and mineralocorticoids produced by the adrenals. Although this is a relatively uncommon disease in the general dog population, some breeds, including the Nova Scotia Duck Tolling Retriever (NSDTR), are at increased risk for developing hypoadrenocorticism. A prior study has shown that the increased risk is due to a heritable component. This potentially lethal disorder is hypothesized to have an autoimmune etiology, thus the aim of this study was to determine whether genetic susceptibility to hypoadrenocorticism in NSDTRs is associated with genes of the canine major histocompatibility complex [MHC; dog leukocyte antigen system (DLA)]. Samples were collected from NSDTRs diagnosed with hypoadrenocorticism ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217541 Thu, 28 Jan 2010 00:00:00 +0100 3217541 http://www.medworm.com/index.php?rid=3217540&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01441.x Cellular assays have been used to assess the pre- and post-transplant immune status of recipients throughout the history of clinical transplantation. Initially, bulk culture assays were used; however, more recent refinements in the techniques allow for assaying multiple functions simultaneously including cell subset functional analysis and the response at the single cell level. The intracellular ATP synthesis assay is being incorporated routinely into the clinical testing strategy. Both donor-specific and non-specific testing strategies are being used to evaluate the immune status of recipients allowing for potential points of intervention aimed at decreasing the adverse impact of clinical complications. This review focuses on the current use of cellular testing in clinical transplantation... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217540 Thu, 28 Jan 2010 00:00:00 +0100 3217540 http://www.medworm.com/index.php?rid=3217539&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01444.x Minor histocompatibility antigens (MiHAgs), such as HA-1 and HA-2, are the main targets of immune responses after allogeneic stem cell transplantation (SCT). HA-1 and HA-2 are two hematopoietic system-restricted antigens encoded, respectively, by HMHA1 and MYO1G genes. In order to estimate their frequencies in Tunisians, we performed a molecular-based allele analysis for 160 healthy and unrelated subjects. Genomic DNAs were extracted mainly by the salting out method. Single nucleotide polymorphism (SNP) genotyping assays for selected sites at HMHA1 gene (rs3764653 and rs1801284) and at MYO1G gene (rs61739531) were performed with a sequence specific primers-polymerase chain reaction (SSP-PCR) method. Statistical analysis of our results showed that the HA-2 antigen is more frequent than the ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217539 Thu, 28 Jan 2010 00:00:00 +0100 3217539 http://www.medworm.com/index.php?rid=3217538&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2010.01452.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3217538 Thu, 28 Jan 2010 00:00:00 +0100 3217538 http://www.medworm.com/index.php?rid=3160504&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01439.x Complement-dependent cytotoxicity or flow cytometric lymphocyte crossmatch (LXM) tests may fail to detect clinically significant antibodies (Abs) against non-human leukocyte antigen (HLA). A flow cytometric endothelial precursor cell crossmatch (EPCXM) test (XM-ONE®) is available for detection of Abs against donor endothelial precursor cells (EPCs). We showed that lymphocytes co-purified with EPCs can be used in LXM tests allowing simultaneous detection of Abs reactive with donor EPCs and lymphocytes. The lymphocyte population co-purified with EPCs on anti-Tie-2 Ab-coupled magnetic beads contained CD 8+ and CD 4+ T-cells, B-cells, and natural killer (NK)- and natural killer T (NKT)-cells. HLA class I antigen expression was slightly higher on CD 3+ lymphocytes co-purified on Tie-2 Ab beads... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3160504 Tue, 12 Jan 2010 00:00:00 +0100 3160504 http://www.medworm.com/index.php?rid=3160503&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01435.x In conclusion, this study indicates that the +14 bp HLA-G allele defines a nearly unique exon 8 haplotype, and fetuses homozygous for this haplotype [SNP 2995(C )/SNP 3127(G )/SNP 3172(A )/SNP 3181(G )/+14 bp] are associated with severe pre-eclampsia in primiparas. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3160503 Tue, 12 Jan 2010 00:00:00 +0100 3160503 http://www.medworm.com/index.php?rid=3160507&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01437.x This study was designed to evaluate the relationship between the presence of tumor necrosis factor (TNF) polymorphisms, human leukocyte antigen (HLA)-DRB1*03 linkage and the prognosis of sarcoidosis. In a retrospective case[ndash]control study, TNF-[alpha]G-308A, TNF-[alpha]G-238A, lymphotoxin-[alpha] (LTA) and HLA-DRB1*03 were genotyped in 625 sarcoidosis patients. These patients were classified into 298 patients with persistent disease and 327 patients with non-persistent disease using chest X-ray (CXR) appearances and lung function parameters after at least 2 years of follow-up. The TNF-[alpha]-308A variant allele was observed in 25.5% of patients with persistent disease compared with 44.0% of patients with non-persistent disease. The corresponding odds ratio (OR) was 0.43 with a 95% co... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3160507 Mon, 11 Jan 2010 00:00:00 +0100 3160507 http://www.medworm.com/index.php?rid=3160506&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01433.x The T-cell immunoglobulin mucin (TIM) gene family encodes receptors on T-cells that regulate Th1- and Th2-cell-mediated immunity. Recently published data implied differential expression of human TIM molecules by mononuclear cells in cerebrospinal fluid of patients with multiple sclerosis (MS) and might therefore be involved in different phases of the pathogenesis of MS. The purpose of this study was to investigate the association of TIM1 gene polymorphism with susceptibility to and clinical progression in MS. In total, 272 patients with MS and 272 sex- and age-matched healthy blood donors from Western Austria were genotyped for 10 single nucleotide polymorphisms (SNPs). Five SNPs were located in the promoter region of TIM1 (rs7702920, rs41297577, rs41297579, rs9313422 and rs34333511). Anot... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3160506 Mon, 11 Jan 2010 00:00:00 +0100 3160506 http://www.medworm.com/index.php?rid=3160505&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01428.x This study analyses the incidence of HLA-DP antibodies in renal patients. Development and epitope specificity of donor-specific antibodies (DSA) and non-DSA (NDSA) were examined. Pre- and posttransplant sera of 338 patients were screened for HLA-DP antibodies using the luminex single antigen assay. Positive patients, partners and/or kidney donors were HLA-DP typed by sequence-specific oligonucleotides. Potential epitopes were mapped by comparing the amino acid sequences of HLA-DP hypervariable regions (HVR) A[ndash]F of recipient, partner and/or donor. Specificities in the sera were aligned to deduce the HVR motif responsible for the antibodies. HLA-DP antibodies were detected in 14% of the patients (48/338). Before transplantation, the antibodies were shown in 23% (10 females and 1 male) ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3160505 Mon, 11 Jan 2010 00:00:00 +0100 3160505 http://www.medworm.com/index.php?rid=3127301&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01436.x Human leukocyte antigen (HLA)-DQA1*0510 has been identified in a Spanish patient diagnosed with celiac disease. DQA1*0510 differs from DQA1*0505/09 by a point mutation at exon 2 producing an amino acid replacement at codon 77 (I > V). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127301 Wed, 30 Dec 2009 00:00:00 +0100 3127301 http://www.medworm.com/index.php?rid=3127300&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01425.x In conclusion, the Norwegian Sami and the non-Sami Norwegians were significantly different at all HLA loci. Our results can be explained by the fact that the two populations have different origins and that the Sami population has remained smaller and more isolated than its neighbors. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127300 Wed, 30 Dec 2009 00:00:00 +0100 3127300 http://www.medworm.com/index.php?rid=3127299&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01430.x Human leukocyte antigen (HLA)-B*4907 was detected in a family from Morocco because of inconsistencies found between molecular and serologic typing results. B*4907 differs from B*490101 by two nucleotide changes in codon 163, producing an amino acid replacement, L>E. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127299 Wed, 30 Dec 2009 00:00:00 +0100 3127299 http://www.medworm.com/index.php?rid=3127298&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01431.x Human leukocyte antigen (HLA)-DRB1*1615 shows one amino acid replacement at codon 86 (G>V ) regarding DRB1*160101. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127298 Wed, 30 Dec 2009 00:00:00 +0100 3127298 http://www.medworm.com/index.php?rid=3127297&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01438.x The human leukocyte antigen (HLA)-G non-classical major histocompatibility complex (MHC) class I molecule was originally described in first-trimester trophoblasts at the fetal[ndash]maternal interface in 1990. Eight years later, the First International Conference on this molecule was inaugurated by Prof Jean Dausset, recipient of the Nobel Prize in Medicine. The Fifth International Conference on HLA-G, held in Paris on July 2009, began with a tribute to Prof Jean Dausset who left us recently. This conference was co-chaired by Dr Edgardo D. Carosella and Prof Hans Grosse-Wilde, included 57 oral presentations and was attended by approximately 140 delegates from 16 countries. We summarize here the major advances on the HLA-G molecule that were reported, including findings on its biological ac... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127297 Wed, 30 Dec 2009 00:00:00 +0100 3127297 http://www.medworm.com/index.php?rid=3127296&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01426.x This study provides preliminary tools for dog leukocyte antigen (DLA) class II haplotype analysis with 102 single nucleotide polymorphisms (SNPs) identified in 14.6 kb and genotyping of 20 of these SNPs to tag haplotypes in 60 dogs with diabetes mellitus and in 49 non-diabetic dogs. The pattern of LD and analysis of SNP patterns indicated combinations of exon 2 alleles have arisen through both recombination and convergence. For exon 2 haplotypes associated with susceptibility or protection from diabetes mellitus, a region of fixed differences in SNPs across the DQ region was observed, suggesting a region outside exon 2 may be implicated in disease association. Four new DQB1 promoter alleles restricted to diabetic dogs were identified, as well as a substitution difference in the X1 box of t... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3127296 Wed, 30 Dec 2009 00:00:00 +0100 3127296 http://www.medworm.com/index.php?rid=3107240&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01424.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3107240 Mon, 21 Dec 2009 00:00:00 +0100 3107240 http://www.medworm.com/index.php?rid=3097454&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01411.x This study investigated the genotypic distribution of proinflammatory cytokines and T-cell negative regulator cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in healthy subjects and AS patients. Genomic DNA was extracted from 143 AS patients and 166 ethnic-matched healthy subjects. Nine polymorphisms within the genes of interleukin-4 (IL-4) ([minus]34T>C, [minus]81A>G, [minus]285C>T and [minus]589T>C), interleukin-6 (IL-6) ([minus]174G>C), interleukin-10 (IL-10) ([minus]592A>C and [minus]819T>C) and CTLA-4 ([minus]318C>T and +49A>G) were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Significantly less AS patients carried the CTLA-4 high-expressing [minus]318 T allele (P = 0.040). The CTLA-4 +49A>G genotypes were associated with cir... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3097454 Fri, 18 Dec 2009 00:00:00 +0100 3097454 http://www.medworm.com/index.php?rid=3097453&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01412.x A novel human leukocyte antigen (HLA)-DRB3*01 allele carrying a HLA-DRB1-specific sequence motive in exon 2 is described. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3097453 Fri, 18 Dec 2009 00:00:00 +0100 3097453 http://www.medworm.com/index.php?rid=3097452&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01427.x In this study, we investigated human leukocyte antigen (HLA)-G*0105N and the 14 bp deletion/insertion polymorphism in exon 8 of the HLA-G gene in 600 individuals from two southern Chinese Han populations (Hunan Han and Guangdong Han) and two northern Chinese populations (Inner Mongolia Han and Inner Mongolia Mongol), we also studied the linkage disequilibrium (LD) between HLA-G and HLA-A locus in these four populations. Our data showed that (1) the allele and haplotype frequencies of HLA-G and HLA-A loci did not differ significantly between the two southern Chinese Han populations, and showed remarkable homogeneity in the two northern Chinese populations; (2) HLA-G*0105N had significantly higher frequencies in the two northern Chinese populations with a frequency of 10.1% in the Inner Mong... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3097452 Fri, 18 Dec 2009 00:00:00 +0100 3097452 http://www.medworm.com/index.php?rid=3085391&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01417.x This is the first report of high-resolution human leukocyte antigen (HLA) typing in four indigenous groups in Malaysia. A total of 99 normal, healthy participants representing the Negrito (Jehai and Kensiu), Proto-Malay (Temuan) and a native group of Borneo (Bidayuh) were typed for HLA-A, -B, -DRB1 and -DQB1 genes using sequence-based typing. Eleven HLA-A, 26 HLA-B, 16 HLA-DRB1 and 14 HLA-DQB1 alleles were detected, including a new allele, HLA-B*3589 in the Jehai. Highly frequent alleles were A*2407, B*1513, B*1801, DRB1*0901, DRB1*1202, DRB1*1502, DQB1*0303 and DQB1*0502. Principal component analysis based on high-resolution HLA-A, -B and -DRB1 allele frequencies showed close affinities among all four groups, including the Negritos, with other Southeast Asian populations. These results sh... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3085391 Tue, 15 Dec 2009 00:00:00 +0100 3085391 http://www.medworm.com/index.php?rid=3072447&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01415.x HLA-B*070209 shows one nucleotide difference from B*070201 in exon 4. HLA-B*130205 has a single nucleotide difference compared with HLA-B*130201. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3072447 Thu, 10 Dec 2009 00:00:00 +0100 3072447 http://www.medworm.com/index.php?rid=3072449&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01404.x Natural killer (NK) cells, a major cell type of the innate immunity, express surface receptors that regulate potent effector functions such as cytolytic activity and release of cytokines playing a central role in inflammatory response and immunoregulation. In this contribution, we briefly outline the major steps from the discovery of human leukocyte antigen (HLA)-class I-specific inhibitory receptors in humans to recent successful clinical applications in the cure of high-risk leukemias both in adults and in pediatric patients. A central role is played by 'alloreactive' NK cells originated from donor's CD 34+ cells in eradicating leukemic cells in the setting of T-cell-depleted haploidentical hemopoietic stem cell transplantation. Because alloreactive NK cells play a central role also in p... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3072449 Wed, 09 Dec 2009 00:00:00 +0100 3072449 http://www.medworm.com/index.php?rid=3072448&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01414.x This investigation was focused on the contribution of individual human leukocyte antigen (HLA)-DR and -DQ alleles to the human hepatitis C virus (HCV)+ non-Hodgkin's lymphoma (NHL), with and without mixed cryoglobulinemia (MC), to study whether individual HLA class II alleles are expressed preferentially or equally in human HCV-specific NHL. For this purpose, peripheral blood mononuclear cells were obtained from two groups of patients with HCV+ NHL and with or without MC (70 and 71 cases, respectively), and from 4575 blood donors. Eighty-three subjects with HCV infection only, and 118 patients with MC, only without lymphoma, were added as additional control groups. Individual HLA-DR and -DQ alleles were determined using high-resolution sequence-based typing and then data were collected by ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3072448 Wed, 09 Dec 2009 00:00:00 +0100 3072448 http://www.medworm.com/index.php?rid=3063965&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01416.x In this study, we characterize two mAbs, 9B9 and 3G8, that recognize the N domain of ACE and that influence shedding and dimerization. Fine epitope mapping was performed, which mapped the epitopes for these mAbs to the N terminal region of the N domain where they overlap to a large extent, despite having different effects on ACE processing. The mAb 3G8 epitope appears to be shielded by the C domain and to be carbohydrate dependent as binding increased significantly as a result of underglycosylation, whereas these factors did not influence mAb 9B9 recognition. Three mutations within the overlapping region of these two epitopes, Q18H, L19E, and Q22A, which decreased mAb 3G8 binding to the soluble N domain, were introduced into full-length somatic ACE (sACE) to determine their influence on AC... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3063965 Tue, 08 Dec 2009 00:00:00 +0100 3063965 http://www.medworm.com/index.php?rid=3063966&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01407.x In this study, we provided the first four digital HLA-A, -B, -C and -DRB1 allele and haplotype data in the Dai ethnic population, which is a unique and representative Kam-Tai-speaking ethnic minority living in the Yunnan province of Southwestern China. Our results showed that the Dai population has unique HLA characteristic that are most closely related to the Southeastern Asia group and similar to the Kam-Tai speaking populations in China and Thailand. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3063966 Mon, 07 Dec 2009 00:00:00 +0100 3063966 http://www.medworm.com/index.php?rid=3045622&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01413.x Description of a new non-human primate major histocompatibility complex (MHC)-B (Patr-B) allele. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=3045622 Wed, 02 Dec 2009 00:00:00 +0100 3045622 http://www.medworm.com/index.php?rid=2990135&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01409.x A total of 93 frozen primary renal cell carcinoma (RCC) samples and 31 frozen samples of corresponding normal renal tissue were analyzed for human leukocyte antigen (HLA) class I and HLA-DR expression. Unexpectedly, HLA class I expression was much higher on RCC cells than on normal renal tubular cells. Immunohistochemistry analysis of frozen and paraffin-embedded tissue samples, applying an extended panel of specific anti-HLA monoclonal antibodies, showed elevated HLA class I antigen expression in 95.6% of the tumors vs only 12.9% of normal renal tissues. These findings were confirmed by molecular analysis of HLA heavy chain and [beta]2-microglobulin ([beta]2m) transcription levels using quantitative real-time polymerase chain reaction (PCR) on microdissected tissue samples (isolated tumor... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2990135 Sat, 14 Nov 2009 00:00:00 +0100 2990135 http://www.medworm.com/index.php?rid=2978448&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01408.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2978448 Wed, 11 Nov 2009 00:00:00 +0100 2978448 http://www.medworm.com/index.php?rid=2978451&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01395.x Intercellular adhesion molecule 1 (ICAM1) gene polymorphisms have been implicated in the susceptibility to inflammatory diseases. The expression of both soluble and tissue ICAM1 were increased in Behçet's disease (BD) but the contribution of ICAM1 gene polymorphisms to this disease remains unknown. We sought to establish the association of ICAM1 gene K469E polymorphism in exon 6 with susceptibility for BD. One hundred and thirty-five Tunisian patients who satisfied the International Study Group criteria for BD and 157 healthy blood donor controls from the same geographic area were genotyped by polymerase chain reaction method for the K469E ICAM1 gene polymorphisms in exon 6. There were no significant differences in the distribution of the K469E allele or genotype frequencies between the B... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2978451 Tue, 10 Nov 2009 00:00:00 +0100 2978451 http://www.medworm.com/index.php?rid=2978450&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01402.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2978450 Tue, 10 Nov 2009 00:00:00 +0100 2978450 http://www.medworm.com/index.php?rid=2978449&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01403.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2978449 Tue, 10 Nov 2009 00:00:00 +0100 2978449 http://www.medworm.com/index.php?rid=2964221&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01396.x Major histocompatibility complex (MHC) class I chain-related gene A (MICA) is located within the human MHC, centromeric to HLA-B and telomeric to HLA-DRB1. The location of MICA in the MHC indicates the presence of linkage disequilibrium with human leukocyte antigen (HLA). Like HLA, MICA is highly polymorphic; however, the information available for MICA polymorphisms is not as comprehensive as that for HLA polymorphisms. We estimated the allelic frequencies of MICA and haplotypes with HLA-B and HLA-DRB1 at high-resolution in a population of 139 unrelated Korean individuals by applying the newly developed method of sequence-based typing (SBT). A total of 17 MICA alleles were identified. The most frequent allele was MICA*010 (19.4%), followed by alleles *00201 (17.6%), *00801 (14.7%), *01201 ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2964221 Fri, 06 Nov 2009 00:00:00 +0100 2964221 http://www.medworm.com/index.php?rid=2964220&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01389.x CD98, a heterodimeric type II transmembrane protein, is involved in many different cellular events, ranging from amino acid transport to cell[ndash]cell adhesion. Little is known about the positive and negative signalling pathways involved in these responses. Therefore, we examined the role of conventional protein kinase C (PKC) isoforms during CD98-induced intracellular signalling and homotypic aggregation of U937 cells. The CD98-induced aggregation was enhanced by the general protein kinase inhibitors GF109203X and staurosporin, and by specific PKC-[alpha]/-[beta] peptide inhibitor 19[ndash]27, but inhibited by PKC activators such as phorbol 12-myristate 13-acetate (PMA). PMA-inhibition was reversed by PKC inhibitors recognising the ATP-binding site in PKC (e.g. staurosporin, GF109203X a... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2964220 Fri, 06 Nov 2009 00:00:00 +0100 2964220 http://www.medworm.com/index.php?rid=2964219&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01394.x The new human leukocyte antigen (HLA)-DRB1 nucleotide sequence differs from HLA-DRB1*150101 in position 130 with an A instead of a T resulting in an amino acid change from Cysteine to Serine. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2964219 Fri, 06 Nov 2009 00:00:00 +0100 2964219 http://www.medworm.com/index.php?rid=2940585&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01390.x This study evaluated the expression and alteration of HLA class I molecules in a panel of 47 pairs of gastric cancer specimens with their noncancerous parts from Chinese patients by using immunohistochemistry (IHC), reverse transcription polymerase chain reaction (RT-PCR) and methylation-specific PCR (MSP) analysis. The expression of HLA-A, HLA-B/C and HLA class I complex was lost or down-regulated in human gastric cancer. The percentage of promoter methylation was 59.57% for HLA-A gene, 55.32% for HLA-B gene and 48.94% for HLA-C gene in gastric cancer, while it was decreased to 19.15%, 12.77% and 6.38% in the adjacent nontumor tissues, respectively. Seven of 10 (70%), 4 of 6 (66.7%) and 3 of 4 (75%) gastric cancer specimens with promoter hypermethylation at HLA-A, -B and -C loci showed tr... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2940585 Fri, 30 Oct 2009 00:00:00 +0100 2940585 http://www.medworm.com/index.php?rid=2935939&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01392.x We determined the alleles of KIR3DL1 and KIR3DS1 in a cohort of British Caucasian ankylosing spondylitis (AS) patients and HLA-B27-positive controls. We found no association in frequencies of the alleles of these genes in AS. In addition, no differences were found when the patients and controls were differentiated by gender. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2935939 Thu, 29 Oct 2009 00:00:00 +0100 2935939 http://www.medworm.com/index.php?rid=2913449&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01373.x HLA-Cw*0361 allele differs from Cw*030301 by one nucleotide substitution at nt 566 from T to A, resulting in an amino acid change at codon 165 from Val to Glu. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913449 Wed, 21 Oct 2009 23:00:00 +0100 2913449 http://www.medworm.com/index.php?rid=2913457&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01374.x Mexicans are the most common minority population of the United States. From a sample of 553 bone marrow donor registrants of self-described Mexican ancestry, human leukocyte antigen (HLA) loci A, C, B and DRB1 were typed by highresolution sequence based typing (SBT) methods. A total of 47, 34, 76 and 46 distinct alleles at A, C, B and DRB1 respectively were identified, including 3 new alleles. The four-locus haplotype frequency distribution was extremely skewed with only 53.9% of 1106 chromosomes present with more than one estimated copy. Haplotypes of Native American origin were identified. These data form an initial basis for determining the requirements for an adequate donor pool for stem cell transplantation in this population. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913457 Tue, 20 Oct 2009 23:00:00 +0100 2913457 http://www.medworm.com/index.php?rid=2913456&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01381.x A sample of 492 full heritage, unrelated residents of the Gila River Indian Community (GRIC) of Arizona were characterized for their high-resolution DNA alleles at the HLA-A, B, C, DRB1, DQA1, and DQB1 loci. Only five allelic categories are found at HLA-A, 10 at HLA-B, 8 at HLA-C and HLA-DR, and 4 at DQA1 and DQB1. There is little evidence for population structure at the 6 loci. Two 'private' alleles, B*5102 and B*4005, which are found nearly exclusively in American Indian populations in the desert southwest and northern Mexico, are likely new mutations after the first inhabitation of the area, the evolution of which are reflected in the contemporary distribution of their respective haplotypes. DRB1*1402 has the highest reported frequency of any specificity at the DRB1 locus, 0.7461, and s... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913456 Tue, 20 Oct 2009 23:00:00 +0100 2913456 http://www.medworm.com/index.php?rid=2913455&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01386.x Three new alleles with polymorphisms located in exons 2 and 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913455 Tue, 20 Oct 2009 23:00:00 +0100 2913455 http://www.medworm.com/index.php?rid=2913454&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01360.x Low-affinity IgE receptor gene (FCER2) rs3760687 polymorphism was found to be associated with differential binding affinity of transcription factors Sp1 and Sp3 leading to altered transcriptional activity. Haplotypic interaction of functional FCER2 polymorphisms (rs28364072, rs2228137 and rs3760687) might potentially provide a background for genotype[ndash]phenotype associations previously observed for some rather non-functional FCER2 variants. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913454 Tue, 20 Oct 2009 23:00:00 +0100 2913454 http://www.medworm.com/index.php?rid=2913453&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01364.x The sequence of novel HLA-Cw*0124 allele differs from Cw*0103 by one nucleotide change at nt 806 from C to A, resulting in an amino acid change at codon 245 from Ala to Glu. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913453 Tue, 20 Oct 2009 23:00:00 +0100 2913453 http://www.medworm.com/index.php?rid=2913452&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01375.x An association between human leukocyte antigen (HLA) molecules and the incidence of various diseases has been recognized for years. Molecules of the HLA system also exert an important role on the clinical outcome after transplantation such as stem cell transplantation. The conventional HLA typing method is sequence based typing (SBT) which is reliable but laborious. The goal of this study was to develop a high-throughput Taqman® assay to screen large panels for HLA-E alleles. Two functional HLA-E alleles, *0101 and *0103, have been identified. We set up the Taqman® assay with genomic DNA as template to discriminate the three HLA-E genotypes: homozygous HLA-E*0101, heterozygous *0101, *0103 and homozygous *0103. This Taqman® approach was validated by the comparison of results obtained wi... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913452 Tue, 20 Oct 2009 23:00:00 +0100 2913452 http://www.medworm.com/index.php?rid=2913451&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01382.x Cytokines are secreted signalling molecules with decisive effects on haematopoiesis, innate and adaptive immunity, and immunopathology. Interleukin (IL)-21 is a novel cytokine produced by activated CD4+ T cells and natural killer T (NKT) cells. IL-21 is part of a family of cytokines which include IL-2, -4, -7, -9 and -15 that all share the common IL-2 receptor [gamma] chain ([gamma]c) in their individual receptor complexes. IL-21 receptor (IL-21R) is widely expressed on both myeloid and lymphoid cell lineages and IL-21 actions include co-stimulation of B cell differentiation and immunoglobulin (Ig) production, co-mitogen of T cells, and stimulation of NK and CD8+ T cell cytotoxic function. Initially, IL-21 was recognized for its anti-tumour effects in several preclinical tumour models, war... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913451 Tue, 20 Oct 2009 23:00:00 +0100 2913451 http://www.medworm.com/index.php?rid=2913450&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01385.x Cw*0774 differs from Cw*070201 by one nucleotide within the coding sequence of exons 2[ndash]4. DQB1*060105 differs from DQB1*060101 by one nucleotide within the coding sequence of exons 2[ndash]3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2913450 Tue, 20 Oct 2009 23:00:00 +0100 2913450 http://www.medworm.com/index.php?rid=2895609&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01383.x Abacavir is a nucleoside reverse transcriptase inhibitor (NRTI) that is used in combination antiretroviral therapy in HIV-infected patients. It is currently recommended as a preferred or an alternative NRTI in antiretroviral-naïve patients. The major toxicity of abacavir is a hypersensitivity reaction (HSR), which occurs in approximately 5% of treated patients. There is a strong association between the human leukocyte antigen (HLA)-B*5701 allele and abacavir HSR, which has allowed for rapid acceptance of genetic screening for HLA-B*5701 in clinical use. Canadian clinicians working in hospital centers with HLA typing capacity opted to launch a pilot project in 2006 to offer the screening test as standard of care to HIV-infected patients. Currently, more than 11,000 HLA-B*5701 tests have be... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2895609 Thu, 15 Oct 2009 23:00:00 +0100 2895609 http://www.medworm.com/index.php?rid=2887669&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01378.x Human leukocyte antigen (HLA) class I sequence-based typing (SBT) for hematopoietic unrelated donor searching in a Romanian Caucasian patient showed the presence of a novel HLA-B allele defined as B*0777. HLA-B*0777 has two nucleotides changes at the same codon from B*0707, resulting an amino acid replacement 99Y > 99S. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2887669 Tue, 13 Oct 2009 23:00:00 +0100 2887669 http://www.medworm.com/index.php?rid=2887670&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01343.x A novel HLA-C allele, Cw*0824, which was identified from an individual of the Han Chinese, differs from Cw*080101 at codon 222 (GAG > AAG ) in exon 4, which results in an amino acid change Glu222Lys. In recent years, many human leukocyte antigen (HLA)-C alleles have been identified. Up to date, 23 different Cw*08 alleles have been identified according to the IMGT/HLA Database release 2.25.2 May 2009 (1). Here, we describe the identification of the novel allele HLA-Cw*0824 that was found during routine high resolution sequence-based typing (SBT) of a Chinese stem cell voluntary donor. The HLA alleles of the donor were typed as A*11, 24; B*15, 46; and DRB1*09, 12. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2887670 Mon, 12 Oct 2009 23:00:00 +0100 2887670 http://www.medworm.com/index.php?rid=2859688&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01384.x The novel HLA-Cw*0130 variant allele differs from the closest allele Cw*010201 by single nucleotide change at genomic nt 959 T>C (CDS nt 583 T>C, codon 171 TAC>CAC) in exon 3, which causes an amino acid change Tyr171His. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2859688 Sun, 04 Oct 2009 23:00:00 +0100 2859688 http://www.medworm.com/index.php?rid=2859692&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01376.x Genomic full-length sequence of HLA-Cw*140201, differing from Cw*1403 by four nucleotide exchanges in exon 2 and intron 5, was identified by cloning and sequencing from a male Chinese donor. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2859692 Sat, 03 Oct 2009 23:00:00 +0100 2859692 http://www.medworm.com/index.php?rid=2859691&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01377.x As part of the 15th International Histocompatibility and Immunogenetics Workshop (IHIWS), seven centers participated in a collaborative project to determine whether any significant humoral sensitization occurred post-transplant among recipients of HLA partially mismatched hematopoietic cell transplants (HCTs). A total of 140 donor/recipient pairs were enrolled with a total of 367 pre-and post-transplant sera analyzed. The majority of the samples (69.1%) were obtained within 30[ndash]90 days post-HCT. HLA-specific antibodies were defined using single antigen bead assays on a Luminex[trade] platform with a positive cutoff value of 1000 normalized median fluorescence intensity (MFI). There was an overall incidence of post-HCT sensitization toward donor HLA mismatches of 5.7%; however, all cas... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2859691 Sat, 03 Oct 2009 23:00:00 +0100 2859691 http://www.medworm.com/index.php?rid=2859690&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01379.x The HLA-B27 subtypes have a varied racial and ethnic prevalence throughout the world. However, the association of B27-subtypes with ankylosing spondylitis (AS) in the mainland China is unknown. To determine the association of B27-subtypes with AS in the Mainland Chinese Han population, a total of unrelated 153 patients with AS were enrolled in a large case-control association study, and 1545 unrelated, healthy, ethnically matched blood donors were included as controls. The genotyping of B27 and its subtypes was performed using the polymerase chain reaction with sequence specific primers (PCR-SSP). A total of 130 (84.97%) AS patients and 61 (3.95%) healthy controls were B27 positive. Three B27-subtypes, B*2704, B*2705 and B*2710, were further identified, of which both B*2704 and B*2705 were... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2859690 Sat, 03 Oct 2009 23:00:00 +0100 2859690 http://www.medworm.com/index.php?rid=2859689&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01380.x A novel HLA-Cw*08 allele, Cw*0822, differing from the closest related allele Cw*080101 by one nucleotide exchange at nt2557 (G>A) in exon 6, was identified by genomic full-length cloning and sequencing from a male Chinese donor. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2859689 Sat, 03 Oct 2009 23:00:00 +0100 2859689 http://www.medworm.com/index.php?rid=2839723&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01361.x A project of the 15th International Histocompatibility Workshop examined the rarity of human leukocyte antigen (HLA) alleles. A section was constructed in the website, www.allelefrequencies.net to contain this data from different sources. A mechanism to search the data was implemented for use by any individual. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2839723 Mon, 28 Sep 2009 23:00:00 +0100 2839723 http://www.medworm.com/index.php?rid=2825571&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01369.x In humans, the region configurations DR1, DR8, DR51, DR52 and DR53 are known to display copy number as well as allelic variation, rendering high resolution typing of HLA-DRB haplotypes cumbersome. Advantage was taken of microsatellite D6S2878, present in all DRB genes/pseudogenes with an intact exon 2-intron 2 segment. This DRB-STR is highly polymorphic in composition and length. Recently, it was proven that all exon 2 sequences could be linked to a certain DRB-STR that segregates with the respective DRB allele. Because haplotypes show differential copy numbers and compositions of exon 2-positive DRB genes/pseudogenes, unique DRB-STR patterns could be described that appear to be specific for a particular DRB haplotype. The aim of this workshop project was to approve and to qualify this sim... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2825571 Wed, 23 Sep 2009 23:00:00 +0100 2825571 http://www.medworm.com/index.php?rid=2825570&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01372.x The complete genomic sequence of HLA-B*1325 allele shows one nucleotide difference from B*130101 at nt 302 where A [rarr] G resulting in an amino acid substitution from Asn(AAC) to Ser(AGC) at codon 77 in exon 2. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2825570 Wed, 23 Sep 2009 23:00:00 +0100 2825570 http://www.medworm.com/index.php?rid=2808577&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01362.x The genomic sequence of HLA-Cw*1222 allele is identical to Cw*120202 except for a single-nucleotide substitution at codon 87 (CAG[rarr]CGG) resulting in an amino acid change from Gln to Arg. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2808577 Fri, 18 Sep 2009 23:00:00 +0100 2808577 http://www.medworm.com/index.php?rid=2808580&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01351.x The full length sequence of HLA-B*3818 differs from HLA-B*380201 at nt 660 in exon 4 (C[rarr]A) and genomic position 2133 in intron 5 (A[rarr]C). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2808580 Thu, 17 Sep 2009 23:00:00 +0100 2808580 http://www.medworm.com/index.php?rid=2808579&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01337.x Genomic studies involving well-defined multicenter cohorts of HIV-1/AIDS covering multiple populations have led to a greater understanding of the role of host determinants in viral acquisition, disease progression, transmission, and response to anti-retroviral therapy. Similarly, recent knowledge on the virus genetic diversity has helped in elucidating mechanisms leading to the evolution of viral escape mutants and the role played by host immune determinants, in particular the major histocompatibility complex (MHC) associated genes. At least two alleles, HLA-B*27 and B*57, have been identified as 'protective' against HIV-1 while B*35 and B*53 act as susceptibility favoring factors. How human leukocyte antigen (HLA)-mediated selection drives the evolution of HIV-1 and which circulating vari... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2808579 Thu, 17 Sep 2009 23:00:00 +0100 2808579 http://www.medworm.com/index.php?rid=2808578&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01352.x Genomic full length sequence of human leukocyte antigen (HLA)-Cw*030301 that differs from Cw*030401 by two-nucleotide exchange at nt 473 (G>A) in exon 2 and nt 3033 (G>C) in 3'UTR, was identified by cloning and sequencing from a male Chinese donor. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2808578 Thu, 17 Sep 2009 23:00:00 +0100 2808578 http://www.medworm.com/index.php?rid=2801762&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01366.x We report here comparison of the KIR gene content and genotypic structure of KIRs in 106 individuals from Eastern mainland Chinese Han and 97 from Taiwanese Han. All 17 KIR genes were observed in the two populations. Framework genes 2DL4, 3DL2, 3DL3 and 3DP1 were present in all individuals. The two populations had very similar frequencies in most loci, however, significant differences were noted in the frequencies of KIR3DS1 and KIR2DS4D (KIR2DS4 deletant variant). A total of 35 and 29 genotypes were identified in the individuals from the Eastern mainland Chinese and the Taiwanese Hans, respectively. Some pairs of KIRs showed significant positive and negative linkage disequilibrium (LD). Our data showed that there were minor distinctions in KIR gene frequencies, genotypes and LD between th... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2801762 Wed, 16 Sep 2009 23:00:00 +0100 2801762 http://www.medworm.com/index.php?rid=2801761&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01371.x Four new HLA class I alleles, Cw*0525, Cw*0825, B*3950 and A*3219N, have been characterized. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2801761 Wed, 16 Sep 2009 23:00:00 +0100 2801761 http://www.medworm.com/index.php?rid=2775821&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01342.x The C allele of a single nucleotide polymorphism (SNP), rs6897932, located in the interleukin-7 receptor alpha chain (IL7RA) was recently found to be associated with multiple sclerosis and Type I diabetes. We analysed 13 SNPs in the IL7RA gene in a combined cohort of patients with chronic inflammatory arthropathies (rheumatoid arthritis and juvenile idiopathic arthritis; 368 patients and 532 unaffected subjects). No significant associations with disease were found with the exception of the non-synonymous SNP rs6897932. This SNP showed modest enrichment of the TT genotype in arthritic patients compared with controls [P = 0.02; OR 1.72 (95% CI 1.08[ndash]2.75)]. Our data are suggestive for a role of rs6897932 in predisposition to chronic inflammatory arthropathies. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2775821 Tue, 08 Sep 2009 23:00:00 +0100 2775821 http://www.medworm.com/index.php?rid=2775822&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01334.x Thirty-six novel human leukocyte antigen (HLA) alleles are described in this article: A*9225N, A*9234, A*030106, A*0337, A*2317, A*2480, A*3023; B*070206, B*0759, B*0761, B*0765, B*150106, B*1827, B*352002, B*3585, B*3943, B*4082, B*5151; Cw*0342, Cw*0343, Cw*0344, Cw*0428, Cw*0430, Cw*0433, Cw*050104, Cw*0519, Cw*060203, Cw*070109, Cw*070202, Cw*0750, Cw*0815, Cw*120306, Cw*1409; DRB1*0336, DRB1*0473 and DRB1*1382. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2775822 Mon, 07 Sep 2009 23:00:00 +0100 2775822 http://www.medworm.com/index.php?rid=2773145&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01340.x The HLA-DRB1*120204 allele differs by a single synonymous nucleotide change from the DRB1*120201 allele at position 203 in exon 2 from C to G. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773145 Mon, 07 Sep 2009 23:00:00 +0100 2773145 http://www.medworm.com/index.php?rid=2773144&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01346.x HLA-Cw*0121 allele was identified in a Chinese patient. It is identical to Cw*0104 except for five nucleotides changes. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773144 Mon, 07 Sep 2009 23:00:00 +0100 2773144 http://www.medworm.com/index.php?rid=2773143&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01347.x This study included a total of 98 patients with RHD as a sequela of ARF, who fulfilled the revised classification criteria of Jones and 154 healthy unrelated controls. CTLA-4 +49 A/G polymorphism was genotyped by using PCR-RLFP technique. Data was analyzed by binary logistic regression models. The frequencies of GG, GA and AA genotypes were found to be 14%, 47% and 39%, respectively, in patients and 6%, 45% and 49%, respectively, in controls. The GG genotype was found to be significantly different between patients and controls (OR: 3.11; P = 0.016). GA and AA genotypes did not statistically differ between patients and controls. Our data showed a significant association of +49G /G polymorphism in a small patient group with RHD. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773143 Mon, 07 Sep 2009 23:00:00 +0100 2773143 http://www.medworm.com/index.php?rid=2773142&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01349.x The full-length sequences of exons 1[ndash]8 of this novel HLA-Cw*070205 allele are identical to those of HLA-Cw*07020101, except for one nucleotide change at nt 498 in exon 3 from C to T, which result in a synonymous amino acid substitution from ATC to ATT at codon 142 in exon 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773142 Mon, 07 Sep 2009 23:00:00 +0100 2773142 http://www.medworm.com/index.php?rid=2773141&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01350.x We here report a new HLA-B allele, B*9521, which differs from B*1544 at codon 67 (TCC[rarr]TGC). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773141 Mon, 07 Sep 2009 23:00:00 +0100 2773141 http://www.medworm.com/index.php?rid=2773140&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01353.x A novel human leukocyte antigen-Cw*04 allele, Cw*04010103, was identified by genomic full length cloning and sequencing from a male Chinese donor. It differs from the closest related allele Cw*04010101 by one nucleotide exchange at nt 1111 (G>A) in intron 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773140 Mon, 07 Sep 2009 23:00:00 +0100 2773140 http://www.medworm.com/index.php?rid=2773139&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01357.x Human neutrophil reactive antibodies may cause clinical disorders such as transfusion-related acute lung injury, febrile transfusion reactions, alloimmune neonatal neutropenia, immune neutropenia after stem cell transplantation, refractoriness to granulocyte transfusion, drug-induced neutropenia and autoimmune neutropenia. Using the granulocyte immunofluorescence test by flow cytometry, the phenotypic frequencies of the human neutrophil alloantigens (HNA)-1a, -1b, -2, -3a and -4a were determined in 100 healthy Brazilian persons. Neutrophils were separated from blood samples by sedimentation, centrifugated and incubated with HNA-specific alloantibody plus fluorescein isothiocyanate-labeled F(ab')2 fragments of anti-human IgG. The results showed that the phenotype frequencies of HNA-1a, -1b,... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2773139 Mon, 07 Sep 2009 23:00:00 +0100 2773139 http://www.medworm.com/index.php?rid=2759300&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01355.x Full length sequences of this novel HLA-B*132202 allele are identical to those of HLA-B*132201 allele, except for a synonymous amino acid substitution from ACG to ACC at codon 138 in exon 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2759300 Wed, 02 Sep 2009 23:00:00 +0100 2759300 http://www.medworm.com/index.php?rid=2646596&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01314.x In this study using 58 single nucleotide polymorphisms (SNPs) with 616 UC cases, there was significant association with SNP rs2294881 of the (butyrophilin-like 2) BTNL2 gene with odds ratio (OR) = 2.80, confidence interval (CI) = 1.62[ndash]4.84 and P = 5.69 × 10[minus]4 (PBonferroni = 3.3 × 10[minus]2) and replication of SNP rs9268480. The missense SNP rs2076523 (K196E) showed novel association with a subset of UC cases with colectomy (n = 126), OR = 0.25, CI = 0.11[ndash]0.58 and P = 4.42 × 10[minus]4 (PBonferroni = 2.56 × 10[minus]2). These three associated variants within the BTNL2 gene were neither in linkage disequilibrium with each other nor correlated with the SNPs tagging the human leukocyte antigen (HLA)-DRB1*1502 and HLA-DRB1*0301 alleles. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2646596 Tue, 28 Jul 2009 23:00:00 +0100 2646596 http://www.medworm.com/index.php?rid=2617382&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01317.x A novel rare variant within the CD59 gene was linked with coeliac disease in a family with high incidence of disease. Functional analyses of this variant were performed using complementary DNA analysis and protein analysis in paraffin-embedded duodenal biopsies from affected individuals and controls. No effects on pre-mRNA or size of linear protein were observed, although these results do not exclude the possible effects of this variant on co-translational protein folding. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2617382 Mon, 20 Jul 2009 23:00:00 +0100 2617382 http://www.medworm.com/index.php?rid=2617381&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01319.x The new allele HLA-A*1141 differs from HLA-A*110101 at the codon 120 by a substitution at the first nucleotide (GGC to CGC). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2617381 Mon, 20 Jul 2009 23:00:00 +0100 2617381 http://www.medworm.com/index.php?rid=2614694&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01312.x Human leukocyte antigen-G (HLA-G) expression is a potential factor for the pathogenesis of virus infection. A 14 bp insertion/deletion polymorphism (rs16375) in the 3'-untranslated region of the HLA-G gene is involved in the stability of HLA-G mRNA and HLA-G protein expression. Therefore, the HLA-G 14 bp polymorphism might be involved in human cytomegalovirus (hCMV) infection. To test a possible association between the HLA-G 14 bp deletion/insertion polymorphism and the active hCMV infection, in this study, a total of 54 patients with active hCMV infection and 165 age- and sex-matched, unrelated, normal Chinese Han population were genotyped for the 14 bp insertion/deletion polymorphism. Association of 14 bp polymorphism with hCMV urine DNA copies and the odds ratio (OR) of the polymorphism... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2614694 Sat, 18 Jul 2009 23:00:00 +0100 2614694 http://www.medworm.com/index.php?rid=2607134&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01321.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607134 Thu, 16 Jul 2009 23:00:00 +0100 2607134 http://www.medworm.com/index.php?rid=2607140&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01306.x We report the identification of a novel B*40 allele, officially named B*400204, found in a Martinican prospective bone marrow donor by means of sequence-based typing techniques. This novel allele differs from the B*400201 allele by a synonymous nucleotide exchange at codon 31 in exon 2 (ACG [rarr] ACC). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607140 Wed, 15 Jul 2009 23:00:00 +0100 2607140 http://www.medworm.com/index.php?rid=2607139&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01311.x Accumulating evidence suggests that alloreactive memory T-cells may be generated as a result of viral infection. So far, a suitable tool to define the individual human leukocyte antigen (HLA) cross-reactivity of virus-specific memory T-cells is not available. We therefore aimed to develop a novel system for the detection of cross-reactive alloresponses using single HLA antigen expressing cell lines (SALs) as stimulator. Herein, we generated Epstein-Barr Virus (EBV) EBNA3A specific CD8 memory T-cell clones (HLA-B*0801/FLRGRAYGL peptide restricted) and assayed for alloreactivity against a panel of SALs using interferon-[gamma] Elispot as readout. Generation of the T-cell clones was performed by single cell sorting based on staining with viral peptide/major histocompatibility complex-specific... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607139 Wed, 15 Jul 2009 23:00:00 +0100 2607139 http://www.medworm.com/index.php?rid=2607138&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01313.x This report describes the discovery and characterization of the human leukocyte antigen-B*4466 allele. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607138 Wed, 15 Jul 2009 23:00:00 +0100 2607138 http://www.medworm.com/index.php?rid=2607137&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01315.x In conclusion, HLA-DRB1*0340 is a new allele with unique properties compared with other known HLA-DRB alleles with regard to antigenicity, T-cell receptor-binding and peptide-binding possibilities. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607137 Wed, 15 Jul 2009 23:00:00 +0100 2607137 http://www.medworm.com/index.php?rid=2607136&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01316.x A novel human leukocyte antigen DRB1 allele, DRB1*1219, has been identified in a Chinese leukaemia patient and his family by polymerase chain reaction sequence-based typing, which has one nucleotide change at position 341 (C[rarr]T) in exon 2 from the closest matching allele DRB1*120201, resulting in an amino acid substitution from Ala[rarr]Val at codon 85. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607136 Wed, 15 Jul 2009 23:00:00 +0100 2607136 http://www.medworm.com/index.php?rid=2607135&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01320.x The rs763361 single nucleotide polymorphism (SNP) within the CD226 gene has recently been reported as a novel susceptible locus for type 1 diabetes. The CD226 gene is implicated in the regulation of a number of cells involved in immune mechanisms leading to [beta]-cell destruction in type 1 diabetes. The aim of the present study was to confirm the association of the CD226 gene with type 1 diabetes in Estonian population. The TT genotype [odds ratio (OR) = 2.29, 95% confidence interval (CI) = 1.25[ndash]4.18, P = 0.0071) and the T allele (OR = 1.48, 95% CI = 1.11[ndash]1.98, P = 0.0084) of the rs763361 SNP were associated with the risk of type 1 diabetes. The current study replicates the novel association of the rs763361 SNP in susceptibility of type 1 diabetes and supports the CD226 gene a... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2607135 Wed, 15 Jul 2009 23:00:00 +0100 2607135 http://www.medworm.com/index.php?rid=2595159&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01310.x The identification of the novel human leukocyte antigen (HLA)-Cw*0524, which was found in a potential stem cell donor, is described. The sequence of the new allele differs from HLA-Cw*0501 and HLA-Cw*0503 by a nonsynonymous amino acid (AA) exchanges at position 49. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2595159 Mon, 13 Jul 2009 23:00:00 +0100 2595159 http://www.medworm.com/index.php?rid=2595161&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01307.x A novel human leukocyte antigen-B allele, officially named B*512402, was identified in a Korean bone marrow donor. The B*512402 allele shows two nucleotide substitutions compared with B*512401 in exon 3 at codons 135 (GCG [rarr] GCC) and 138 (ACC [rarr] ACG) without any amino acid substitution. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2595161 Sun, 12 Jul 2009 23:00:00 +0100 2595161 http://www.medworm.com/index.php?rid=2595160&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01309.x Three nucleotide changes and one insertion in intron 2 upon human leucocyte antigen (HLA)-G*01010105 generate HLA-G*01010106. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2595160 Sun, 12 Jul 2009 23:00:00 +0100 2595160 http://www.medworm.com/index.php?rid=2515684&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01304.x Mycobacterium tuberculosis, the causal agent of pulmonary tuberculosis (TB), remains a major health problem throughout the world causing high mortality in humans. Previous studies showed that several genes may play crucial roles in susceptibility to TB. The PTPN22 gene encodes the lymphoid tyrosine phosphatase that has an important regulatory effect on T- and B-cell activation in immune response. The purpose of this study was to investigate the role of two functional missense single nucleotide polymorphisms (SNPs) of the PTPN22 gene region (R620W and R263Q) in the susceptibility to TB in the Moroccan population. A case[ndash]control association study was performed including 123 pulmonary TB patients and 155 healthy controls. All subjects were genotyped by TaqMan SNP genotyping assays. Rega... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2515684 Thu, 25 Jun 2009 23:00:00 +0100 2515684 http://www.medworm.com/index.php?rid=2515687&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01293.x A new human leukocyte antigen DRB1*0908 differs from GTC to AGC in DRB1*090102 at codon 57 with a coding change, valine to serine. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2515687 Wed, 24 Jun 2009 23:00:00 +0100 2515687 http://www.medworm.com/index.php?rid=2515686&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01301.x Mycobacterium tuberculosis is one of the most successful of human pathogens and has acquired the ability to establish latent or progressive infection and persist even in the presence of a fully functioning immune system. The ability of M. tuberculosis to avoid immune-mediated clearance is likely to reflect a highly evolved and coordinated program of immune evasion strategies, including some that interfere with antigen presentation to prevent or alter the quality of T-cell responses. Here, we review an extensive array of published studies supporting the view that antigen presentation pathways are targeted at many points by pathogenic mycobacteria. These studies show the multiple potential mechanisms by which M. tuberculosis may actively inhibit, subvert or otherwise modulate antigen present... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2515686 Wed, 24 Jun 2009 23:00:00 +0100 2515686 http://www.medworm.com/index.php?rid=2515685&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01303.x Cytotoxic T lymphocyte antigen 4 (CTLA4; CD152) is a costimulatory molecule expressed on activated T cells that plays a key inhibitory role during T lymphocyte activation. The gene encoding for CTLA4 has been suggested as a candidate for conferring susceptibility to autoinflammatory diseases. We investigated the polymorphisms of the CTLA4 gene [promoter region ([minus]1722 T/C, [minus]1661 A/G and [minus]318 C/T) and exon 1 (+49 G/A)] and the differences of serum soluble sCTLA4 levels in 285 patients with Behcet's disease (BD) and 287 controls. The frequency of the CTLA4 [minus]1661 GG genotype was significantly higher in BD patients than in controls [P = 0.019, odds ratio (OR) = 5.2, 95% confidence interval (CI) = 1.13[ndash]23.86]. Also, the genotype frequency for CTLA4 [minus]1722 TC wa... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2515685 Wed, 24 Jun 2009 23:00:00 +0100 2515685 http://www.medworm.com/index.php?rid=2478775&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01302.x In this study, 109 RCC primary lesions (clear cell, n = 95; chromophobe, n = 4; papillary, n = 4; collecting duct, n = 6) and corresponding adjacent tumor-negative renal tissues (n = 34) were analyzed for the HLA-G expression by immunohistochemistry (IHC). Meanwhile, plasma soluble HLA-G (sHLA-G) from 16 RCC patients and 144 sex- and age-matched normal individuals was detected by enzyme-linked immunosorbent assay. Correlations between lesion HLA-G expression and various clinical parameters were evaluated. Receiver-operating characteristic (ROC) curve analysis was used to determine the feasibility of HLA-G protein staining and sHLA-G as a diagnosis marker for RCC. IHC data showed that HLA-G was observed in 49.5% of clear cell, 50% of either chromophobe or collecting duct RCC lesions but und... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2478775 Tue, 16 Jun 2009 04:00:00 +0100 2478775 http://www.medworm.com/index.php?rid=2478776&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01292.x Human leukocyte antigen-DRB1*090202 has two synonymous nucleotide substitutions with DRB1*090201 at codons 57 and 58, which may be the result of a gene conversion. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2478776 Mon, 15 Jun 2009 04:00:00 +0100 2478776 http://www.medworm.com/index.php?rid=2474607&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01298.x Polymorphisms from the TENR-IL2-IL21 block in the 4q27 chromosome were recently associated with type 1 diabetes, celiac disease, rheumatoid arthritis and psoriasis. We undertook this study to investigate the potential role of polymorphisms rs3136534, rs6822844 and rs2069762 ([minus]330 T/G IL2) in multiple sclerosis (MS) (805 patients of Spanish Caucasian origin and 952 health controls). We did not find evidence for association with any single nucleotide polymorphisms (SNPs) tested. Allele and genotype frequencies of the SNPs, which were studied, were similar in DRB1*15-positive or DRB1*15-negative patients. After stratification of MS patients by clinical course, a weak association was observed with rs2069762 G allele and haplotype bearing this allele with secondary progressive MS, althoug... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474607 Sun, 14 Jun 2009 04:00:00 +0100 2474607 http://www.medworm.com/index.php?rid=2474610&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01295.x DRB1*1532 allele was identified in a Mexican unrelated marrow donor from the Gulf of Mexico. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474610 Thu, 11 Jun 2009 04:00:00 +0100 2474610 http://www.medworm.com/index.php?rid=2474609&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01291.x In 1958, just a little more than 50 years ago, an alloantigen present on human leucocytes was detected, which was to become the 'first' human leucocyte antigen (HLA); HLA-A2. Since then, we have seen a tremendous development of the HLA field, which has moved from histocompatibility to become one of the most central fields in basic and clinical immunology. This development is briefly reviewed in this article, focusing on some highlights of the history of HLA class I and II molecules and their role in immune responses. It is emphasized that the quick and extensive development of the HLA field is the result not only of excellent individual contributions by outstanding pioneers in the field, but also of an extensive international collaboration, in particular through the many international hist... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474609 Thu, 11 Jun 2009 04:00:00 +0100 2474609 http://www.medworm.com/index.php?rid=2474608&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01296.x Twenty-six novel human leukocyte antigen (HLA) class I alleles are described: 3 HLA-A alleles, 19 HLA-B alleles and 4 HLA-C alleles. Only one of the novel alleles (HLA-B*0753) was found in multiple individuals and likely is not uncommon in the population. Nineteen ([sim]70%) of the 26 novel alleles are single nucleotide substitution variants when compared with their most homologous allele. Four of these single nucleotide variants are silent substitutions, and one creates a null allele. The remaining novel alleles differ from their most similar allele by two to six nucleotide substitutions. Some of the new alleles encode novel codons and unique amino acid changes at polymorphic positions in the HLA-B lows (codons 30, 67 and 72), while HLA-Cw*0347 encodes an amino acid change at a position n... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474608 Thu, 11 Jun 2009 04:00:00 +0100 2474608 http://www.medworm.com/index.php?rid=2474611&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01294.x Here, we report the identification of a novel human leukocyte antigen-DRB1*12 variant, DRB1*1218 allele, in a Chinese Han individual. The novel DRB1*12 variant allele differed from the closest allele DRB1*120201 by nucleotide 262 G>C (codon 59 GAG>CAG) missense mutation in exon 2, which resulted in an amino acid substitution of Glu>Gln. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474611 Wed, 10 Jun 2009 04:00:00 +0100 2474611 http://www.medworm.com/index.php?rid=2474619&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01260.x Human leukocyte antigen (HLA)-DRB1*1611 has one nucleotide change at codon 14 (GAG[rarr]AAG) from DRB1*160201, resulting in a coding change from Glu to Lys. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474619 Thu, 04 Jun 2009 04:00:00 +0100 2474619 http://www.medworm.com/index.php?rid=2474618&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01269.x A novel human leukocyte antigen (HLA)-A allele has been identified in a Chinese individual. HLA-A*290102 allele is different from HLA-A*29010101 by one nucleotide silent substitution at position 448T>C in exon 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474618 Thu, 04 Jun 2009 04:00:00 +0100 2474618 http://www.medworm.com/index.php?rid=2474617&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01278.x A new human leukocyte antigen-Cw*0401 allele showed one nucleotide difference from Cw*04010101 in exon 3 at nucleotide position 561 (codon 163 ACG[rarr]ACT) without a coding change. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474617 Thu, 04 Jun 2009 04:00:00 +0100 2474617 http://www.medworm.com/index.php?rid=2474616&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01282.x Birth weight is known to be a direct indicator of perinatal mortality and a clear predictor of adult pathologies too. It has been correlated with several causes of mortality in adulthood: low birth weight with diabetes, nephropathy and cardiovascular diseases and high birth weight with autoimmune diseases and cancer. In genome-wide studies, an extended human leucocyte antigen (HLA) region has been linked to birth weight variation. We focused our attention on the HLA haplotypes marked by HLA-A, HLA-B and HLA-DRB1 polymorphisms in 1206 healthy Caucasian newborns belonging to the Cord Blood Bank of Pavia (Italy) and their mothers, aiming to investigate the association between this restricted HLA region and birth weight variation. In our study, the HLA-B*38;DRB1*13 haplotype showed an ascendin... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474616 Thu, 04 Jun 2009 04:00:00 +0100 2474616 http://www.medworm.com/index.php?rid=2474615&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01284.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474615 Thu, 04 Jun 2009 04:00:00 +0100 2474615 http://www.medworm.com/index.php?rid=2474614&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01285.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474614 Thu, 04 Jun 2009 04:00:00 +0100 2474614 http://www.medworm.com/index.php?rid=2474613&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01288.x (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474613 Thu, 04 Jun 2009 04:00:00 +0100 2474613 http://www.medworm.com/index.php?rid=2474612&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01289.x In this study, we report the identification of three new human leukocyte antigen class I alleles: A*2493, A*2918 and A*0342 found by routine typing using commercial kits. The names A*2493 (HWS 10005702), A*2918 (HWS 10005703) and A*0342 (HWS 10005705) have been officially assigned by the World Health Organization Nomenclature Committee in August 2008. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474612 Thu, 04 Jun 2009 04:00:00 +0100 2474612 http://www.medworm.com/index.php?rid=2474627&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01271.x Although the determination of human leukocyte antigen (HLA) antibody specificity has traditionally been directed toward HLA antigens, there is now increasing attention to structurally defined HLA epitopes. An understanding of the HLA epitope repertoire is important to acceptable mismatching for sensitized patients and to a new epitope-based matching algorithm aimed to reduce antibody-mediated rejection. There are two strategies to determine the HLA epitope repertoire. Terasaki's group has used an empirical method to analyze the reactivity of single allele Luminex panels with mouse monoclonal antibodies (mAbs) and absorbed/eluted alloantibodies with a computer program based on shared residues in the amino acid sequences of reactive alleles. HLAMatchmaker is a theoretical algorithm that pred... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474627 Tue, 02 Jun 2009 04:00:00 +0100 2474627 http://www.medworm.com/index.php?rid=2474626&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01272.x This study demonstrates how the HLAMatchmaker interpretation of amino acid residues shared between antibody-reactive antigens can increase our understanding of the structural basis of HLA epitopes. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474626 Tue, 02 Jun 2009 04:00:00 +0100 2474626 http://www.medworm.com/index.php?rid=2474625&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01276.x Cytokines and intercellular adhesion molecule (ICAM) play a crucial role in the pathogenesis of primary kidney disease and progression to end-stage renal disease (ESRD). Cytokine secretion is reported to be dependent on the single nucleotide polymorphisms located in the cytokine genes. The role of different polymorphisms in cytokines and ICAM genes as probable susceptibility factors for ESRD has been explored in the present study. The study was conducted on 258 ESRD patients and on ethnically matched 569 controls. Individuals were genotyped for interleukin (IL)-6 (G174C), IL-4 (C590T), tumor necrosis factor-[alpha] (TNF-[alpha]) ([minus]G308A and [minus]G238A) and ICAM-1 (A469G) gene polymorphisms using standard polymerase chain reaction[ndash]restriction fragment length polymorphism[ndash... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474625 Tue, 02 Jun 2009 04:00:00 +0100 2474625 http://www.medworm.com/index.php?rid=2474624&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01281.x This study is the first report of HLA-B*15 subtypes in Burmese. These results will provide the basic data in the further study in transplantations, genetic association with diseases, and drug hypersensitivity. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474624 Tue, 02 Jun 2009 04:00:00 +0100 2474624 http://www.medworm.com/index.php?rid=2474623&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01283.x In conclusion, pretransplant DSA detected by the LumXm had no impact on AR episodes. Class II LumXm positivity proved no significant risk factor for graft failure, but the value of the class II LumXm is questionable. A positive class I LumXm resulted in worse long-term graft survival compared with a negative one. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474623 Tue, 02 Jun 2009 04:00:00 +0100 2474623 http://www.medworm.com/index.php?rid=2474622&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01286.x One nucleotide difference was shown at position 594 in exon 3 from C to T between human leukocyte antigen (HLA)-B*550203 and HLA-B*550201, which does not alter amino acids in the mature protein. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474622 Tue, 02 Jun 2009 04:00:00 +0100 2474622 http://www.medworm.com/index.php?rid=2474621&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01287.x The novel human leukocyte antigen-Cw*0821 allele differs from the closest allele Cw*080101 by single nucleotide change at genomic DNA nucleotide 419 A>G (codon73 ACT>GCT) in exon 2, which results in an amino acid change Thr73Ala. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474621 Tue, 02 Jun 2009 04:00:00 +0100 2474621 http://www.medworm.com/index.php?rid=2474620&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01290.x A novel human leukocyte antigen-A allele, A*2489, was identified in a Chinese registered donor. The A*2489 allele differs from the closest matching allele A*2428 by two nucleotide substitutions, 257(A[rarr]G) and 265(G[rarr]C), in exon 2. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474620 Tue, 02 Jun 2009 04:00:00 +0100 2474620 http://www.medworm.com/index.php?rid=2474629&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01277.x A novel human leukocyte antigen (HLA) allele, HLA-Cw*0820, was identified in a Chinese Han individual. It differs from the closest allele Cw*080101 by single nucleotide change at genomic nucleotide (nt) 1615 G>A (coding sequence nt 652 G>A, codon 194 GTC>ATC) in exon 4, which results in an amino acid change Val194Ile. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474629 Wed, 27 May 2009 04:00:00 +0100 2474629 http://www.medworm.com/index.php?rid=2474628&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01279.x New allele B*0769 showed one nucleotide difference with B*0732 at codon 272 (TTC[rarr]TGC). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474628 Wed, 27 May 2009 04:00:00 +0100 2474628 http://www.medworm.com/index.php?rid=2474630&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01268.x In this paper, we describe the novel human leukocyte antigen (HLA)-B*1832 allele that we found in a female Spanish volunteer blood donor for clinical investigation during her high-resolution HLA typing. The HLA-B typing is B*1801, 1832, and the DNA sequence is homozygous with the exception characterized by a nucleotide exchange 'C' to 'A' at position 505, which, in consequence, replaced arginine at codon 169 (CGC) by serine in the new allele B*1832. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474630 Wed, 20 May 2009 04:00:00 +0100 2474630 http://www.medworm.com/index.php?rid=2474633&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01266.x The novel allele human leucocyte antigen (HLA)-Cw*0746 differs from HLA-Cw*070101 by three nucleotide exchanges at codons 45 and 66 in exon 2 and at codon 99 in exon 3. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474633 Mon, 04 May 2009 04:00:00 +0100 2474633 http://www.medworm.com/index.php?rid=2474632&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01267.x Human leukocyte antigen (HLA)-DRB1*150205 shows one nucleotide difference from HLA-DRB1*150201 at position 357 in exon 2 from A to G and does not result in an amino acid changes. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474632 Mon, 04 May 2009 04:00:00 +0100 2474632 http://www.medworm.com/index.php?rid=2474631&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2009.01270.x We report here the sequence of a new human leucocyte antigen-A2 allele, A*9251, identified in a volunteer haematopoietic stem cell donor of the international registry. A*9251 differs from A*02010101 by two nucleotides at codons 113[ndash]114, resulting in a single His>Asp substitution at codon 114. (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474631 Mon, 04 May 2009 04:00:00 +0100 2474631 http://www.medworm.com/index.php?rid=2474634&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1399-0039.2008.01208.x We report the identification of the novel allele HLA-Cw*0760 that was found during confirmatory high-resolution sequence-based typing of a bone marrow donor. The Cw*0760 allele has two nucleotide changes from Cw*070101 at positions 341 and 343 in exon 2, resulting in two amino acid changes from Asp to Ala (codon 90: GAC[rarr]GCC) and Gly to Arg (codon 91: GGG[rarr]AGG). (Source: Tissue Antigens) Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2474634 Tue, 21 Apr 2009 04:00:00 +0100 2474634 http://www.medworm.com/index.php?rid=2303913&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19317737%26dopt%3DAbstract Authors: Kaur G, Mehra N Interindividual variability in susceptibility to HIV-1 infection, its transmission, disease progression, and response to antiviral therapy has been attributed to host determinants and variability in multiple genes. Although most people exposed to the virus go on to develop full-blown disease at variable intervals, a proportion of them, labeled as long-term nonprogressors or exposed uninfected, possess 'natural resistance' to infection. A better understanding of genetic and immunologic basis of such a natural resistance to infection would bear important implications in designing therapeutic vaccine designs. The genetic variants that could influence susceptibility to HIV-1 and limit AIDS vary in different populations and among individuals. Meta-analyses of large ... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2303913 Wed, 01 Apr 2009 04:00:00 +0100 2303913 http://www.medworm.com/index.php?rid=2303911&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19317738%26dopt%3DAbstract Authors: Wu J, Fu S, Ren X, Jin Y, Huang X, Zhang X, Bai J, Fu S Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that plays an important role in pathogenesis of asthma. A high level of MIF has been detected in bronchoalveolar lavage fluid, serum and sputum in asthma. Polymorphisms associated with inflammatory diseases exist in the promoter region of MIF, which alter its expression. The aim of this study was to evaluate the potential relationship between functional polymorphisms of MIF and childhood asthma in a northeastern Chinese population. The study consisted of a set of 41 trios and an independent sample set of 189 childhood asthma patients and 261 healthy controls. We genotyped MIF-173G/C using polymerase chain reaction-restriction fragment length polymo... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2303911 Wed, 01 Apr 2009 04:00:00 +0100 2303911 http://www.medworm.com/index.php?rid=2303909&cid=s_33167_3_f&fid=33167&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19317739%26dopt%3DAbstract This report summarizes the new swine leukocyte antigen (SLA) allele sequences and haplotypes designated by the SLA Nomenclature Committee of the International Society for Animal Genetics. There have been 74 new SLA alleles, comprising 18 SLA-1 alleles, 11 SLA-2 alleles, six SLA-3 alleles, two SLA-6 alleles, one SLA-DRA allele, 20 SLA-DRB1 alleles, three SLA-DQA alleles and 13 SLA-DQB1 alleles. Twelve new SLA class I and four new class II haplotypes have also been designated. This is the first official update since the 2005 reports on the nomenclature for factors of the SLA class I and II systems. This report also summarizes recent updates to the Immunopolymorphism Database-Major Histocompatibility Complex (IPD-MHC) website (http://www.ebi.ac.uk/ipd/mhc/sla/). All information has now been i... Tissue Antigens journals http://www.medworm.com/rss/comments.php?id=2303909 Wed, 01 Apr 2009 04:00:00 +0100 2303909