MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Tissue and Cell' source. Tue, 07 Feb 2012 08:49:02 +0100 http://www.medworm.com/index.php?rid=5623422&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22244242%26dopt%3DAbstract Authors: Riccillo FL, Bracamonte MI, Montenegro S, Martínez SM, Ronderos JR Abstract The eSMT rat is a new spontaneous model of type 2 diabetes that develops a progressive diabetic syndrome with a stronger incidence in males than in females. We decide to investigate the progression of the pancreatic histopathological changes during the lifespan of the eSMT rat, especially those associated with islet cell populations. Besides that, some plasmatic parameters were evaluated in order to correlate them with the morphological findings. Male eSMT and Sprague-Dawley control rats were used. The results showed a dramatic decrease of the volume density (VD) of endocrine tissue in the eSMT rats without evidence of insulitis. Islets became fragmented structures with strong presence of intersti... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5623422 Wed, 11 Jan 2012 05:00:00 +0100 5623422 http://www.medworm.com/index.php?rid=5578602&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22218110%26dopt%3DAbstract Authors: Yang X, Zhao L, Zhao Z, Hu B, Wang C, Yang Z, Cheng Y Abstract Estrogen induces oocytes development and vitellogenesis in crustacean by interacting with estrogen receptor (ER) subtypes. In the present study, we detect for the first time the ERα in oocytes and follicle cells and hepatopancreas cells of mysis by immunohistochemistry using a specific ERα antibody. ERα was mainly localized in the nuclei of oocytes and follicle cells, while mainly detected in nuclei of oogonia (OG), previtellogenic oocyte (PR) and endogenous vitellogenic oocyte (EN) at previtellogenic and early vitellogenic stage (I-early III). Follicle cells in all stages of ovary (all vitellogenic stages) showed strong ERα positive reaction, and they were able to gradually move to oocytes during the devel... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5578602 Mon, 02 Jan 2012 05:00:00 +0100 5578602 http://www.medworm.com/index.php?rid=5550481&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22197619%26dopt%3DAbstract Authors: Wulf-Goldenberg A, Keil M, Fichtner I, Eckert K Abstract In vivo studies concerning the function of human hematopoietic stem cells (HSC) are limited by relatively low levels of engraftment and the failure of the engrafted HSC preparations to differentiate into functional immune cells after systemic application. In the present paper we describe the effect of intrahepatically transplanted CD34(+) cells from cord blood into the liver of newborn or adult NOD/SCID mice on organ engraftment and differentiation. Analyzing the short and long term time dependency of human cell recruitment into mouse organs after cell transplantation in the liver of newborn and adult NOD/SCID mice by RT-PCR and FACS analysis, a significantly high engraftment was found after transplantation into live... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5550481 Fri, 23 Dec 2011 05:00:00 +0100 5550481 http://www.medworm.com/index.php?rid=5535388&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22185679%26dopt%3DAbstract Authors: Dimitrova M, Ivanov I, Todorova R, Stefanova N, Moskova-Doumanova V, Topouzova-Hristova T, Saynova V, Stephanova E Abstract Dipeptidyl peptidase IV (DPPIV) was studied in three human lung cells - P (fetal lung-derived cells), A549 (lung adenocarcinoma) and SK-MES-1 (squamous cell carcinoma) using a fluorescent cytochemical procedure developed on the basis of the substrate 4-(glycyl-l-prolyl hydrazido)-N-hexyl-1,8-naphthalimide. The observed differences in the enzyme expression were confirmed by measuring the enzyme hydrolysis of glycyl-l-prolyl-para-nitroanilide. The surface and total dipeptidyl peptidase activities of P cells were correspondingly 7-8 and 3-10 times higher than those of SK-MES-1 and A549 cells. The ratio surface per total activity showed that in P (95%) an... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5535388 Sun, 18 Dec 2011 05:00:00 +0100 5535388 http://www.medworm.com/index.php?rid=5535387&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22185680%26dopt%3DAbstract Authors: Danišovič L, Varga I, Polák S Abstract The main purpose of the article is to review recent knowledge about growth factors and their effect on the chondrogenic differentiation of mesenchymal stem cells under in vitro conditions. Damaged or lost articular cartilage leads to progressive debilitation, which have major impact on the life quality of the affected individuals of both sexes in all age groups. Mature hyaline cartilage has a very low self-repair potential due to intrinsic properties - lack of innervation and vascular supply. Another limiting factor is low mitotic potential of chondrocytes. Small defects are healed by migration of chondrocytes, while large ones are healed by formation of inferior fibrocartilage. However, in many cases osteoarthritis develops. Recen... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5535387 Sun, 18 Dec 2011 05:00:00 +0100 5535387 http://www.medworm.com/index.php?rid=5535389&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22178208%26dopt%3DAbstract In this study, we attempted to compare the stability of neural differentiation in human ADSCs by using two induction protocols. Isolated ADSCs were induced into neural-like cells using diverse effects of two specific procedures. For protocol 1, ADSCs were induced by chemical induction. In protocol 2, ADSCs were treated for sphere formation. Then, the singled cells were cultured in neurobasal media supplemented with special components. Differentiated ADSCs were evaluated for Nestin, MAP2 and GFAP expression by immunocytochemistry and semi quantitative RT-PCR techniques. Moreover, MTT assay was employed to detect cell viability and proliferation. Immunocytochemical analysis of both protocols demonstrated that ADSCs had large expression of the neural-specific markers. In RT-PCR, protocol 1 sh... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5535389 Fri, 16 Dec 2011 05:00:00 +0100 5535389 http://www.medworm.com/index.php?rid=5535390&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22153985%26dopt%3DAbstract Authors: Thomé RG, Domingos FF, Santos HB, Martinelli PM, Sato Y, Rizzo E, Bazzoli N Abstract Aiming to better understand folliculogenesis, this study evaluated cell death and proliferation of ovarian cells, besides cathepsin-D expression in Prochilodus argenteus captured in two sites of the São Francisco River downstream from the Três Marias Dam, Brazil. In the site immediately following the Dam (S1), low levels of dissolved oxygen were registered in the rainy period. The water temperature was higher in the São Francisco River immediately after the confluence with the Abaeté River (S2), regardless of the period. In S1, the ovaries showed smaller oocytes, high caspase-3 enzymatic activity and apoptosis, lower cells in proliferation and GSI, as well as a lesser quantity of cath... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5535390 Tue, 06 Dec 2011 05:00:00 +0100 5535390 http://www.medworm.com/index.php?rid=5493911&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22129754%26dopt%3DAbstract This study examines, for the first time, spatiotemporal changes in the centriolar complex in various phases of spermiogenesis in a passerine bird, the Masked weaver (Ploceus velatus). It also describes the configuration of the centriolar complex and the relationship between it and the granular body in both intra- and extra-testicular spermatozoa. It is shown that the proximal centriole is retained and attaches, at its free end, to the granular body of spermatids in every step of spermiogenesis, as well as in mature intra-testicular and post-testicular spermatozoa, including those in the lumen of the seminal glomus. As the centriolar complex, along with its attached granular body, approaches the nucleus in the early spermatid, the proximal centriole articulates with the distal centriole at ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5493911 Mon, 28 Nov 2011 05:00:00 +0100 5493911 http://www.medworm.com/index.php?rid=5493910&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22129755%26dopt%3DAbstract This study examined the expression of AQP-1 and AQP-9 in adult epididymis of the UChA and UChB 10% (v/v) ethanol-preferring rats, focusing the ethanol-induced hormonal disturbances upon the regulation of these AQPs. Chronic ethanol intake significantly decreased body weight, while UChA and UChB rats displayed a marked loss of epididymal weights. Both ethanol-consuming animals had a severe reduction of testosterone levels, whereas LH and 17β-estradiol were unchanged. Throughout the epididymis, a strong reaction to AQP-1 was observed in myoid and endothelial cells of the UChB ethanol-preferring rats, differently from a moderate intensity in the initial segment of the UChA rats. In addition, AQP-9 showed a strong immunoreaction in the apical membrane of principal cells at initial segment. In... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5493910 Mon, 28 Nov 2011 05:00:00 +0100 5493910 http://www.medworm.com/index.php?rid=5421771&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22088868%26dopt%3DAbstract Authors: Foata J, Quilichini Y, Greani S, Marchand B Abstract The ultrastructural organization of the spermatozoon of a cryptogonimid digenean, Aphallus tubarium, a parasite of Dentex dentex, is described. The spermatozoon possesses the elements found in other digeneans: two axonemes with 9+"1" pattern, a mitochondrion, a nucleus, cortical microtubules, external ornamentation and spine-like bodies. However, the mitochondrion appears as a cord with a bulge; this characteristic has never been described in other studied cryptogonimid and in other digeneans except in one lepocreadiid, Holorchis micracanthum. Likewise, the presence of a thin cytoplasm termination in the anterior part of the spermatozoon has never been pointed out in the cryptogonimids. PMID: 22088868 [PubMed - as su... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5421771 Mon, 14 Nov 2011 05:00:00 +0100 5421771 http://www.medworm.com/index.php?rid=5421772&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22079203%26dopt%3DAbstract Authors: Ozegbe PC, Aire TA, Deokar MS Abstract The cellular composition of the testicular capsule, seminiferous peritubular tissue, the epithelia as well as periductal muscle cell layers of the excurrent ducts was studied, in sexually mature and active Masked Weaver (Ploceus velatus) birds of the passerine family, Ploceidae. Ultrastructure of the contractile cells in the testicular capsule, peritubular and periductal tissues showed that these cells were smooth muscles of typical morphological characteristics. Variability in the immunohistochemical co-expression of microfilaments and intermediate filaments in the different tissues was evident. Actin and desmin proteins were co-expressed immunohistochemically in the testicular capsule and seminiferous peritubular smooth muscle layer... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5421772 Fri, 11 Nov 2011 05:00:00 +0100 5421772 http://www.medworm.com/index.php?rid=5421773&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22071216%26dopt%3DAbstract Authors: Izu Y, Ezura Y, Mizoguchi F, Kawamata A, Nakamoto T, Nakashima K, Hayata T, Hemmi H, Bonaldo P, Noda M Abstract Bone consists of type I collagen as a major protein with minor various matrix proteins. Type VI collagen is one of bone matrix proteins but its function is not known. We therefore examined the effects of type VI collagen deficiency on bone. 3D-μCT analysis revealed that type VI collagen deficiency reduced cancellous bone mass. Cortical bone mass was not affected. Type VI collagen deficiency distorted the shape of osteoblasts both in the cancellous bone and in the cambium layer of periosteal region. Furthermore, type VI collagen deficiency disorganized collagen arrangement. These data indicate that type VI collagen contributes to maintain bone mass. PMID: 220... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5421773 Mon, 07 Nov 2011 05:00:00 +0100 5421773 http://www.medworm.com/index.php?rid=5421774&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22056322%26dopt%3DAbstract Authors: Fanciulli PP, Zizzari ZV, Frati F, Dallai R Abstract The initial part of the ejaculatory duct of Orchesella villosa contains a "valve" and a "sorter" avoiding respectively the reflow and allowing the separation of the secretion for the spermatophore stalk from the sperm fluid. For most of its length, the ejaculatory duct lumen is divided into two parts: in the dorsal part the sperm fluid flows while in the ventral district the secretion for the stalk occurs. Laterally, on both sides of the duct, longitudinal muscle fibers are present. The epithelium of the dorsal region consists of two types of long secretory cells; the most peculiar of them are those provided with extracellular cisterns flowing directly into the duct lumen as it occurs in 1st type of epidermal cells. Thes... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5421774 Fri, 04 Nov 2011 04:00:00 +0100 5421774 http://www.medworm.com/index.php?rid=5329368&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22000907%26dopt%3DAbstract In conclusion, RA alleviates GS nephrotoxicity via antioxidant activity, increase of renal GSH content and increase of renal antioxidant enzymes activity. PMID: 22000907 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5329368 Wed, 12 Oct 2011 04:00:00 +0100 5329368 http://www.medworm.com/index.php?rid=5329370&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21996552%26dopt%3DAbstract Authors: Bekheet SH, Abdel-Motaal FF, Mahalel UA Abstract The antifungal efficacy of Ficus sycomorus and Pergularia tomentosa plant extracts on Bufo regularis experimentally infected with Aspergillus niger was studied. After an oral administration of the pathogen for 15 days, the blood, kidney and liver were examined. Treatment with A. niger produced a reduction in red blood count cells and hemoglobin content. Also, both livers and kidneys revealed marked destruction and degenerative changes. These changes included congestion of blood vessels, leukocytic infiltration, and cytoplasmic vacuolization of the hepatocytes. As well as complete destruction of the cellular boundaries of the tubular epithelia, inflammatory leukocytes between the intertubular spaces, destruction and necrosis ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5329370 Tue, 11 Oct 2011 04:00:00 +0100 5329370 http://www.medworm.com/index.php?rid=5329369&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21996553%26dopt%3DAbstract Authors: Al-Ghanbousi R, Ba-Omar T, Victor R Abstract Aphanius dispar, a freshwater fish (mean total length±sd, 3.42±0.33cm) was exposed to different concentrations of deltamethrin (2.25, 2.50 and 3.00μgL(-1)), an insecticide used to control Dubas bug, Ommatissus lybicus in Oman. The histopathological changes in the structure of the gills in fish exposed to deltamethrin was studied using light, transmission and scanning electron microscopy. In light microscopy, the fish exposed to 2.25μgL(-1) deltamethrin, showed hypertrophy and hyperplasia of chloride cells, while in 2.50μgL(-1), additional changes like vacuolization, lifting of the lamellar epithelium and fusion of secondary lamellae were observed. The most severe alteration including vacuolization, desquamation of cells and... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5329369 Tue, 11 Oct 2011 04:00:00 +0100 5329369 http://www.medworm.com/index.php?rid=5247386&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21937067%26dopt%3DAbstract Authors: Adami ML, Damborenea C, Ronderos JR Abstract Mechanisms coordinating cell-cell interaction have appeared early in evolution. Allatotropin (AT), a neuropeptide isolated based on its ability to stimulate the synthesis of juvenile hormones (JHs) in insects has also been found in other invertebrate phyla. Despite this function, AT has proved to be myotropic. In the present study we analyze its expression in two groups of Turbellaria (Catenulida, Macrostomida), and its probable relationship with muscle tissue. The results show the presence of an AT-like peptide in the free living turbellaria analyzed. The analysis of the expression of the peptide together with phalloidin, suggests a functional relationship between the peptide and muscle tissue, showing that it could be acting a... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5247386 Mon, 19 Sep 2011 04:00:00 +0100 5247386 http://www.medworm.com/index.php?rid=5247385&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21937068%26dopt%3DAbstract In this study, we discuss the biological significance of all these features and conclude that the appearance of precursor (intermediate) molecules facilitated the development of complex patterns of condensation and, as a consequence, a great diversity of forms in the sperm cell nuclei. PMID: 21937068 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5247385 Mon, 19 Sep 2011 04:00:00 +0100 5247385 http://www.medworm.com/index.php?rid=5247387&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21930289%26dopt%3DAbstract Authors: Tirapelli LF, Batalhão ME, Jacob-Ferreira AL, Tirapelli DP, Carnio EC, Tanus-Santos JE, Queiroz RH, Uyemura SA, Padovan CM, Tirapelli CR Abstract In the present work we evaluated the effect of ethanol consumption in histopathological liver changes and several biochemical biomarkers employed in the detection of hepatic dysfunction. Male Wistar rats were treated with ethanol 20% (vol/vol) for 6 weeks. Histopathological investigation of livers from ethanol-treated animals revealed steatosis. Indices of hepatic function (transaminases) and mitochondrial respiration were not altered in ethanol-treated rats. Chronic ethanol consumption did not alter malondialdehyde (MDA) levels in the liver. Ethanol consumption induced a significant increase on hepatic nitrite and nitrate level... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5247387 Sat, 17 Sep 2011 04:00:00 +0100 5247387 http://www.medworm.com/index.php?rid=5219280&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899869%26dopt%3DAbstract Authors: Huang J, Wang S, Wei C, Xu Y, Wang Y, Jin J, Teng G Abstract A hybrid hydrogel (PP) composed of Polomaxer-407 (PO) and octapeptide with amino acid sequence of KFEFKFEF (PE) was prepared to make a scaffold material incorporating PO's high and tunable mechanical strength and integrity with PE's superior bioactivity. Human adipose-derived mesenchymal stem cells (hASCs) were encapsulated into PE, PO and PP hydrogels respectively and injected subcutaneously at the dorsal neck area of nude mice. Adipose-like tissue regeneration was only observed in the mice injected with cell-encapsulated PP hydrogel. No adipose regeneration was found in the mice injected with PO or PE. Immunohistochemistry analysis with mouse anti-human nuclei monoclonal antibody demonstrated that the cells in ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5219280 Mon, 05 Sep 2011 04:00:00 +0100 5219280 http://www.medworm.com/index.php?rid=5176755&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872288%26dopt%3DAbstract Authors: Polazzi E, Alibardi L Abstract An in vitro system of isolated skin cells has been developed in order to address the understanding on the factors that control the shedding cycle and differentiation of lizard epidermis. The skin from the regenerating lizard tail has been separated in epidermis and dermis, cells have been dissociated, cultivated in vitro, and studied ultrastructurally after 1-30 days of culture condition. Dissociated keratinocytes after 12 days in culture show numerous cell elongations and contain bundles of keratin or sparse keratin filaments. These cells often contain one to three 0.5-3μm large and dense "keratinaceous bodies", an organelle representing tonofilament disassembling. Most keratinocytes have sparse tonofilaments in the cytoplasm and form short... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5176755 Wed, 24 Aug 2011 23:00:00 +0100 5176755 http://www.medworm.com/index.php?rid=5176754&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21872289%26dopt%3DAbstract This study was designed to investigate this effect within 3 days in 4 groups: control (untreated), chemical, chemical-mechanical and mechanical based on exposure of ASCs to chemical growth factors for 3 days or to mechanical strain just on the 2nd day. Finally, cell orientation, muscle-related gene expression, myosin protein synthesis and the number of myosin-positive cells were examined to estimate the rate of differentiation. By studying the cells before and after exposure to uniaxial strain, it could be observed that by exerting the load, the cells were organized almost perpendicularly to strain direction. Real-time RT-PCR demonstrated that uniaxial strain had a significant effect on up-regulation of muscle-related genes in chemical-mechanical group (P<0.001) as compared to mechanica... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5176754 Wed, 24 Aug 2011 23:00:00 +0100 5176754 http://www.medworm.com/index.php?rid=5157520&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21862094%26dopt%3DAbstract In conclusion, BPF markedly ameliorated cadmium-induced liver and kidney tissue damage as evidenced by histological and biochemical examinations and acts as a potent scavenger of free radicals to protect the liver and kidney against the deleterious effect of acute cadmium intoxication. PMID: 21862094 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5157520 Fri, 19 Aug 2011 23:00:00 +0100 5157520 http://www.medworm.com/index.php?rid=5141664&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21824635%26dopt%3DAbstract Authors: Muthu Rama Krishnan M, Shah P, Choudhary A, Chakraborty C, Paul RR, Ray AK Abstract In the field of quantitative microscopy, textural information plays a significant role very often in tissue characterization and diagnosis, in addition to morphology and intensity. The aim of this work is to improve the classification accuracy based on textural features for the development of a computer assisted screening of oral sub-mucous fibrosis (OSF). In fact, a systematic approach is introduced in order to grade the histopathological tissue sections into normal, OSF without dysplasia and OSF with dysplasia, which would help the oral onco-pathologists to screen the subjects rapidly. In totality, 71 textural features are extracted from epithelial region of the tissue sections using vari... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5141664 Fri, 05 Aug 2011 23:00:00 +0100 5141664 http://www.medworm.com/index.php?rid=5141665&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21820692%26dopt%3DAbstract Authors: Diehlmann A, Bork S, Saffrich R, Veh RW, Wagner W, Derst C Abstract The promising use of mesenchymal stromal cells (MSC) in regenerative technologies accounts for necessity of detailed study of their physiology. Proliferation and differentiation of multipotent cells often involve changes in their metabolic state. In the present study, we analyzed the expression of ATP-sensitive potassium (K(ATP)) channels in MSC and upon in vitro differentiation. K(ATP) channels are present in many cells and regulate a variety of cellular functions by coupling cell metabolism with membrane potential. Kir6.1, Kir6.2 and SUR2A were expressed in undifferentiated MSC, whereas SUR2B and SUR1 were not detected on cDNA and protein level. Upon adipogenic differentiation Kir6.1 and SUR2A showed a s... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5141665 Wed, 03 Aug 2011 23:00:00 +0100 5141665 http://www.medworm.com/index.php?rid=5058180&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764094%26dopt%3DAbstract Authors: Trovato M, Mazzei V, Sinatra F, Longo G Sperm of Armadillidium peraccae have been examined with cytochemical and immunocytochemical methods for fluorescence and electron microscopic visualization of cytoskeleton components. Sperm incubation in an antibody anti-β-tubulin shows only the presence of two centrioles located in the cytoplasmic region above the nucleus; no other microtubules are present in the sperm head. Instead, fluorescence microscopy of sperm incubated in FITC-phalloidin allowed to detect the presence of a large amount of F-actin in the apical region of the sperm head. The incubation of ultrathin sections of sperm embedded in Lowicryl K4M with a phalloidin-gold complex allowed a more precise localization of F-actin in the amorphous part of the acrosome and in th... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5058180 Thu, 14 Jul 2011 23:00:00 +0100 5058180 http://www.medworm.com/index.php?rid=5058179&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764095%26dopt%3DAbstract Authors: Xu C, Chen X, Chang C, Wang G, Wang W, Zhang L, Zhu Q, Wang L, Zhang F To examine the changes of biological activities in hepatic oval cells (HOCs) elicited by 70% partial hepatectomy (PH) and understand the relationship between this cell and liver regeneration (LR), this study isolated and obtained the high purity HOCs (≥95%) from rat regenerating livers, and then monitored gene expression profiling of rat hepatic oval cells following surgical operation. Results showed that there were LR-related 1059 genes. These genes were grossly classified into three groups using a fold change cut-off threshold of three-fold: up-regulation, down-regulation and up/down regulation. Analyses of gene expression patterns combined with gene functional categorization suggested that genes in the... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5058179 Thu, 14 Jul 2011 23:00:00 +0100 5058179 http://www.medworm.com/index.php?rid=5058178&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764096%26dopt%3DAbstract Authors: Ban Y, Wu YY, Yu T, Geng N, Wang YY, Liu XG, Gong P The process of mechanotransduction of bone, the conversion of a mechanical stimulus into a biochemical response, is known to occur in osteoblasts in response to fluid shear stress. In order to understand the reaction of osteoblasts to various times of flow perfusion, osteoblasts were seeded on three-dimensional scaffolds, and cultured in the following conditions: continuous flow perfusion, intermittent flow perfusion, and static condition. We collected samples on day 4, 8 and 12 for analysis. Osteoblast proliferation was demonstrated by cell proliferation and scanning electron microscopy assay. Additionally, the expression of known markers of differentiation, including alkaline phosphatase and osteocalcin, were tested by qRT-... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5058178 Thu, 14 Jul 2011 23:00:00 +0100 5058178 http://www.medworm.com/index.php?rid=5009170&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21705035%26dopt%3DAbstract In this report, we demonstrated for the first time, the major cell types and composition in the guinea pig airway epithelium, using cell type-specific markers by immunohistochemical staining using the commercial available immunological reagents that cross-react with guinea pig. Our results revealed the availability of antibodies cross-reacting with airway epithelial cell types of basal, non-ciliated columnar, ciliated, Clara, goblet and alveolar type II cells, as well as those cells expressing Mucin 5AC, Mucin 2, Aquaporin 4 and Calcitonin Gene Related Peptide. The distribution of these various cell types were quantified in the guinea pig airway by immunohistochemical staining and were comparable with morphometric studies using an electron microscopy assay. Moreover, this study also demons... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5009170 Thu, 23 Jun 2011 23:00:00 +0100 5009170 http://www.medworm.com/index.php?rid=5009171&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21704350%26dopt%3DAbstract Authors: Wu G, Song Y, Zheng X, Jiang Z We aimed to investigate the application of adipose-derived stromal cells in the treatment of stress urinary incontinence (SUI). Animal models of stress urinary incontinence were established with Sprague-Dawley female rats by complete cutting of the pudendal nerve. Rat adipose-derived stromal cells were isolated, cultured and successfully transplanted into animal models. Effects of stem cell transplantation were evaluated through urodynamic testing and morphologic changes of the urethra and surrounding tissues before and after transplantation. Main urodynamic outcome measures were measured. Intra-bladder pressure and leak point pressure were measured during filling phase. Morphologic examinations were performed. Transplantation of adipose-derived ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5009171 Wed, 22 Jun 2011 23:00:00 +0100 5009171 http://www.medworm.com/index.php?rid=5009172&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21703655%26dopt%3DAbstract Authors: Vitale DG, Brundo MV, Viscuso R Ultrastructural investigations on the amphigonic reproductive mechanisms in Aphidoidea (Homoptera, Sternorryncha) species, of which little is known in the literature, can provide useful information on their reproductive biology. Morphological and ultrastructural investigations were carried out on the reproductive tract and on spermatozoa from sexually mature males of five species belonging to three subfamilies of Aphidoidea. The organization of the reproductive tract and of spermatozoa appears simple and similar in the examined species. Each testis consists of three follicles containing many cysts arranged in a progressive order of maturation from the distal to the proximal tract; spermiogenesis only occurs in sexually mature males, ending with ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5009172 Tue, 21 Jun 2011 23:00:00 +0100 5009172 http://www.medworm.com/index.php?rid=5009173&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21700305%26dopt%3DAbstract Authors: Eun LY, Song H, Choi E, Lee TG, Moon DW, Hwang D, Byun KH, Sul JH, Hwang KC Mesenchymal stem cells (MSCs) have been used with success in several clinical applications for clinical treatment of ischemic hearts. However, the reported effects of MSC-based therapy on myocardial infarction (MI) are inconsistent. In particular, the preventive effects of MSC-based therapy on arrhythmic sudden death and metabolic disorders after infarction remain controversial. Here, we investigated the effects of MSCs on reverse remodeling in an infarcted myocardium, and found that MSC-therapy failed to achieve the complete regeneration of infarcted myocardium. Histological analyses showed that although infarct size and interstitial fibrosis induced by MI recovered significantly after MSC treatment, ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=5009173 Mon, 20 Jun 2011 23:00:00 +0100 5009173 http://www.medworm.com/index.php?rid=4962116&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21696798%26dopt%3DAbstract Authors: Colozza G, Montembault E, Quénerch'du E, Riparbelli MG, D'Avino PP, Callaini G Nuclear Pore Complexes (NPCs) are involved in the regulation of nucleo-cytoplasmic trafficking. Drosophila Nup154 encodes a nucleoporin component of the NPC that is expressed in high proliferating tissues such as germ cells. Hypomorphic mutations in this gene cause male and female sterility and reduction of cell proliferation in the adult fly. Here, we present evidences of a decrease in the number of spermatogonial cells in Nup154 mutants, caused both by increased cell death and reduced cell proliferation. Furthermore, we also found that RNAi-mediated depletion of Nup154 in cultured cells prevented nuclear accumulation of the transcription factor Mothers against Dpp (Mad), suggesting a possible reg... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4962116 Sun, 19 Jun 2011 23:00:00 +0100 4962116 http://www.medworm.com/index.php?rid=4813747&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21550620%26dopt%3DAbstract Authors: Challa SS, Kiran GS, Bhonde RR, Venkatesan V The promise(s) of using Fetal Calf Serum (FCS) as a supplement for the maintenance of cell cultures has been well documented. However, FCS forms the xenogenic source for any human derived cells/organ and limits its application. Recently, the usage of human umbilical cord blood serum (hUCBS) for maintenance of mesenchymal cells has been supportive. In the present study we investigated the effects of hUCBS and FCS on the proliferation (viability, proliferative) and its differentiation potential (DTZ staining, immunofluroscence) to generate islet like cellular aggregates (ICAs) using the human derived Panc-1 cell lines. A comparative analysis of hUCBS and FCS for each parameter demonstrated that hUCBS supplemented media was better for ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4813747 Thu, 05 May 2011 23:00:00 +0100 4813747 http://www.medworm.com/index.php?rid=4813746&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21550621%26dopt%3DAbstract Authors: Mashimo Y, Machida R, Dallai R, Gottardo M, Mercati D, Beutel RG The structural features of eggs of Zorotypus caudelli Karny are described in detail. The egg is elliptic with long and short diameters of 0.6 and 0.3mm respectively, and creamy white. The egg shows a honeycomb pattern on its surface, without any specialized structures for hatching such as an operculum or a hatching line. The fringe formed by a fibrillar substance secreted after the completion of the chorion encircles the lateral surface. The egg layer is composed of an exochorion, an endochorion, and a vitelline envelope. The exochorion and endochorion are electron-dense and homogeneous in structure. The exochorion shows a perforation of numerous branching aeropyles. The exo- and endochorion are connected by nume... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4813746 Thu, 05 May 2011 23:00:00 +0100 4813746 http://www.medworm.com/index.php?rid=4759491&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21511319%26dopt%3DAbstract Authors: Bogolyubova IO Embryos of inbred mouse strains stop their cleavage at the late 2-cell stage and this phenomenon is known as the "2-cell block in vitro". In the present work we used microinjections of 5-bromouridine 5'-triphosphate (BrUTP) to examine the transcriptional status of blocked embryos. We also traced nuclear distribution of hyperphosphorylated form of RNA polymerase II in such embryos. We found that transcriptional activity is gradually decreased when the embryos stay in block. This is accompanied by prominent increasing of the size of SC35 nuclear speckles that begin to accumulate hyperphosphorylated RNA polymerase II. PMID: 21511319 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4759491 Mon, 18 Apr 2011 23:00:00 +0100 4759491 http://www.medworm.com/index.php?rid=4759490&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21511320%26dopt%3DAbstract Authors: Morishita M, Ono N, Miyai K, Nakagawa T, Hanyu R, Nagao M, Kamolratanakul P, Notomi T, Rittling SR, Denhardt DT, Kronenberg HM, Ezura Y, Hayata T, Nakamoto T, Noda M Parathyroid hormone/parathyroid hormone-related protein receptor (PPR) signaling is known to be involved in tooth development. In bone, extracellular matrix protein osteopontin (OPN) is a negative regulator of PPR signaling in bone formation. However, the role of OPN in modulation of PPR action in tooth development is not understood. Therefore, we examined the tooth in double mutant mice. Constitutively active PPR was expressed specifically in the odontoblasts and osteoblasts (caPPR-tg) in the presence or absence of OPN. Radiographic analysis indicated that the length of the third molar (M3) and the incisor was de... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4759490 Mon, 18 Apr 2011 23:00:00 +0100 4759490 http://www.medworm.com/index.php?rid=4759489&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21511321%26dopt%3DAbstract Authors: Bala K, Ambwani K, Gohil NK Human umbilical vein endothelial cells (HUVEC) were cultured in two different media, viz. the commonly used M199 containing 20% fetal bovine serum (FBS) and endothelial cell growth factor and a defined media EGM-2 containing 2% FBS along with growth supplements in known concentrations. The purpose of this study was to determine the effect of different media on the growth potential and cell morphology in subsequent passages. We have established that a dual coating of gelatin and human fibronectin extracellular matrix provides optimal cell attachment. Growth rate for primary culture was almost double in defined media. For secondary culture a two fold higher proliferation rate was observed in defined EGM-2 media. Histological studies were done using ph... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4759489 Mon, 18 Apr 2011 23:00:00 +0100 4759489 http://www.medworm.com/index.php?rid=4759492&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21501852%26dopt%3DAbstract Authors: Mekkawy IA, Mahmoud UM, Sayed AE In the present work, the destructive effects of the 4-nonylphenol on one of the most economically important Nile fishes, namely African catfish (Clarias gariepinus) were studied. Apoptosis, erythrocytes alterations, micronucleus test and blood parameters count were used as biological indicators to detect those effects. After exposure to sublethal concentrations of 4-nonylphenol (0, 0.05, 0.08 and 0.1mg/l), apoptotic red blood cells with many malformations and micronucleated erythrocytes were recorded. Decrease in the blood parameters such as red blood cells (RBCs), hemoglobin (Hb), package cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), platelets, white blood cells (WBCs), lymphocytes, basophils, monocytes and increase in m... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4759492 Sat, 16 Apr 2011 23:00:00 +0100 4759492 http://www.medworm.com/index.php?rid=4705961&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21477831%26dopt%3DAbstract Authors: Phoungpetchara I, Tinikul Y, Poljaroen J, Chotwiwatthanakun C, Vanichviriyakit R, Sroyraya M, Hanna PJ, Sobhon P We found that the androgenic gland (AG) of Macrobrachium rosenbergii possesses three cell types. Type I cells are small polygonal shaped-cells (13.4μm in diameter), stain strongly with hematoxylin-eosin (H&E), have abundant multilayered rough endoplasmic reticulum (rER), and nuclei containing mostly heterochromatin. Type II cells are slightly larger (18.6μm in diameter), stain lightly with H&E, have rER with dilated cisternae, and nuclei containing mostly euchromatin. Type III cells (previously undescribed) are similar in size and shape to type I cells, but the cytoplasm is unstained and they have a high amount of smooth endoplasmic reticulum (sER) and mit... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705961 Wed, 06 Apr 2011 23:00:00 +0100 4705961 http://www.medworm.com/index.php?rid=4705970&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470647%26dopt%3DAbstract Authors: Ding BA, Pirone A, Lenzi C, Xiaoming N, Baglini A, Romboli I We demonstrated for the first time the distribution and morphology of argyrophil and of goblet cells in the mucosa of the small intestine of the Muscovy duck during development using the Grimelius silver staining and alcian blue/periodic acid-Schiff (AB/PAS) staining technique. The argyrophil cells distribution was variable over the length of the small intestine from embryonic day 24 (24E) to post-hatching day 13 (13d). In the villi most argyrophil cells belonged to the open-type, while in the crypts they belonged to the closed-type. In the duodenum the density of argyrophil cells was highest at hatching, while in the jejunum and in the ileum the highest density value was at hatching and 13d. AB/PAS-positive goblet c... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705970 Sun, 03 Apr 2011 23:00:00 +0100 4705970 http://www.medworm.com/index.php?rid=4705966&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21470648%26dopt%3DAbstract Authors: Bu XD, Li N, Tian XQ, Huang PL To compare the differences in MUC2 and MUC5AC mRNA among four colon cancer cell lines and to identify the best in vitro models for studying mucin expression, quantitative real-time polymerase chain reaction was used to measure the expression of MUC2 and MUC5AC mRNA in Caco-2, HT29, LoVo, and LS174T cell lines. The levels of MUC2 mRNA expression in the four colon cancer cell lines ranked in order of mRNA abundance were: LS174T>LoVo>HT-29>Caco-2. In contrast to MUC2, the abundances of MUC5AC mRNA were in the order: Caco-2>HT-29>LS174T>LoVo. Caco-2 (highest level of MUC5AC mRNA) and LS174T (highest level of MUC2 mRNA) were used to investigate the phenotypes. Morphologically, Caco-2 cells were larger with low electron density mucus-... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705966 Sun, 03 Apr 2011 23:00:00 +0100 4705966 http://www.medworm.com/index.php?rid=4705977&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21466888%26dopt%3DAbstract Authors: Del Rio-Zaragoza OB, Fajer-Ávila EJ, Almazán-Rueda P, Abdo de la Parra MI The aim of the present study was to obtain a basic knowledge of the hematology in order to determine changes in blood parameters of the spotted rose snapper Lutjanus guttatus. The morphological features of blood cells were described according to the observations made by light microscopy of Wright-Giemsa-stained blood films. The reference intervals and the mean value were determined for each hematological parameter evaluated in healthy fish and data were compared to those of naturally infected, with dactylogyrid monogeneans fish. Infected fish showed a prevalence of 100% and a mean intensity of 246.6 parasites per fish. Mean values of HCT, WBC, thrombocytes percentage and eosinophils percentage were sig... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705977 Sat, 02 Apr 2011 23:00:00 +0100 4705977 http://www.medworm.com/index.php?rid=4705979&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21459396%26dopt%3DAbstract In conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. PMID: 21459396 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705979 Fri, 01 Apr 2011 23:00:00 +0100 4705979 http://www.medworm.com/index.php?rid=4705978&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21459397%26dopt%3DAbstract Authors: Donnerer J, Liebmann I A fluorescence-immunohistochemical investigation was performed in lumbar dorsal root ganglia (DRGs) neurons of the rat with regard to ERK1/2-, p38- and STAT3-phosphorylation in response to nociceptor activation in the rat. The stimuli applied were perineural capsaicin treatment of the sciatic nerve, mustard oil application to the hind paw and heat or cold stimulation of the hind paw. The time points of investigations were 15min/30min after perineural capsaicin, 30min/2h/4h for mustard oil, 10min/4h for cold and 30min/2h/8h for the heat stimulus. All four stimuli lead to a time-dependent, significant 2-3 fold increase in the number of small and medium size DRG cells displaying cytoplasmic staining for p-ERK1/2, but to no activation of satellite cells. Pho... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4705978 Thu, 31 Mar 2011 23:00:00 +0100 4705978 http://www.medworm.com/index.php?rid=4530424&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21349566%26dopt%3DAbstract In this study, we synthesized a new cell immobilization support having poly(glycidyl methacrylate) as a graft polymer chain and used this support for cell cultivation. Base polymer particle was synthesized by suspension polymerization and epoxy polymer chain was extended from particle surface on graft polymerization. Produced polymer particles had broad particle size distribution ranging from 20 to 1000μm and the degree of polymerization of grafted polymer chain was ranged from 500 to 1000. The effects of various factors, such as grafted polymer chain length and its surface density, composition of base polymer network and graft polymer chain, on the cell growth of murine fibroblast cell line (MS-5 cell) on polymer particle were studied. This polymer particle could cultivate not only fibro... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530424 Tue, 22 Feb 2011 00:00:00 +0100 4530424 http://www.medworm.com/index.php?rid=4530425&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21342699%26dopt%3DAbstract Authors: Lupetti P, Mencarelli C, Mercati D, Gaino E, Dallai R Sperm bundles of the ephemeropteran Cloeon dipterum (L.) were found in the seminal vesicle. They are apically protected by cyst cells and sperm anterior regions are embedded in a secretion produced by these cells. Cyst cells further degenerate making sperm cells free. The sperm bundles are able to perform a forward progression only when sperm are stick together with their tail posterior tips. On the contrary, when sperm have their tail ends free, they beat but are unable to progress. The functional significance of the sperm bundles is discussed. PMID: 21342699 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530425 Sun, 20 Feb 2011 00:00:00 +0100 4530425 http://www.medworm.com/index.php?rid=4530426&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334706%26dopt%3DAbstract Authors: Dallai R, Mercati D, Giusti F, Gottardo M, Carapelli A Endosymbionts of the Cardinium-like genus are described in the testes and other tissues of the proturan Acerella muscorum (Ionescu). Few endosymbionts are present in the large apical cells of functional testes, but they become numerous at the end of the reproductive cycle. They are also found within sperm cells where induce their degeneration. The Gram-negative endosymbionts are characterized by the presence of microtubule-like structures (MLC) in their cytoplasm. It is suggested a possible role of the endosymbionts in the elimination of degenerating sperm cells when the testes activity is ended, thus somewhat playing a role in the timing of the reproductive cycle of the proturan species. PMID: 21334706 [PubMed - as su... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530426 Fri, 18 Feb 2011 00:00:00 +0100 4530426 http://www.medworm.com/index.php?rid=4530427&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334036%26dopt%3DAbstract Authors: Tripathi S, Srivastav AK Male Wistar rats were randomly divided into three groups - A, B and C. A dose of 5mg and 10mg of cadmium chloride/kg body weight/day was orally administered to groups B and C, respectively. Rats from group A served as control. Rats were sacrificed on 1st, 2nd, 4th, 6th, and 8th week after initiation of the experiment. Kidneys were removed immediately, fixed in Bouin's fixative, routinely processed and stained with hematoxylin and eosin. The present study showed that the histopathological changes were caused in kidney of rats by cadmium exposure. The changes noticed were mainly - the glomerular swelling (at initial stage), the shrinkage of glomerulus (at later stage), the tubular dilatation, hypertrophy of tubular epithelium, degeneration of glomerulus ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530427 Thu, 17 Feb 2011 00:00:00 +0100 4530427 http://www.medworm.com/index.php?rid=4530428&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329953%26dopt%3DAbstract Authors: Hildebrandt C, Büth H, Thielecke H For studying cellular processes three-dimensional (3D) in vitro models are of a high importance. For tissue engineering approaches osseous differentiation is performed on 3D scaffolds, but material depending influences promote cellular processes like adhesion, proliferation and differentiation. To investigate developmental processes of mesenchymal stem cells without cell-substrate interactions, self-contained in vitro models mimicking physiological condition are required. However, with respect to scientific investigations and pharmaceutical tests, it is essential that these tissue models are well characterised and are of a high reproducibility. In order to establish an appropriate in vitro model for bone formation, different protocols are co... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530428 Tue, 15 Feb 2011 00:00:00 +0100 4530428 http://www.medworm.com/index.php?rid=4530431&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21324499%26dopt%3DAbstract Authors: Martinez M, De Oliveira SA, Pinheiro PF, Almeida-Francia C, Pereira S, Martins OA, Mello-Júnior W, Mendes LO, Chuffa LG, Tirapelli LF, Fávaro WJ, Cagnon VH, Martinez FE The study analyzed the effects of chronic alcohol ingestion on the ultrastructure of the lining epithelium of the hard palatine mucosa of rats UChA and UChB (lines with voluntary alcohol consumption) in order to contribute to the understanding of the consequences of alcohol abuse for the morphology of the digestive system. Thirty female adult animals aged 120 days were divided into three experimental groups. (1) Ten UChA rats (genetically low ethanol consumer) with voluntary intake of 10% v/v (5.45g/kg/day) ethanol solution and water. (2) Ten UChB (genetically high ethanol consumer) rats with voluntary intake... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530431 Mon, 14 Feb 2011 00:00:00 +0100 4530431 http://www.medworm.com/index.php?rid=4530430&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21324500%26dopt%3DAbstract Authors: Niitsu S, Lobbia S, Kamito T Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) underwent apolysis and then cell differentiation. To investigate whether juvenile hormone (JH) in involved in sex-specific cellular response to ecdysteroid during wing development between sexes of E. variegata, we tested the effects of juvenile hormone analog (JHA), methoprene, and 20E on wing disc morphogenesis between sexes in vitro. Using transmission electron microscopy (TEM), we found that both higher concentration of JHA (5μg/ml)... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530430 Mon, 14 Feb 2011 00:00:00 +0100 4530430 http://www.medworm.com/index.php?rid=4530429&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21324501%26dopt%3DAbstract Authors: Lv H, Fu S, Wu G, Yan F The mutation of phosphate-regulating gene with homologies to endopeptidases on the X-chromosome (PHEX) can lead to human X-linked hypophosphatemic rickets which displays hypo-mineralization in bone and dentin. To study its possible roles in teeth, PHEX antibody was injected into pregnant mice on E15 to explore its roles on the formation of enamel and dentin. Mallory trichrome staining results showed that arrangements of ameloblasts and odontoblasts were irregular after PHEX antibody treatment. Differentiation of odontoblasts and the formation of dentin were inhibited. Spatiotemporal distribution of PHEX protein was observed in various stages of tooth germ. Immunohistochemical results showed positive PHEX signals appeared in the inner enamel epithelium o... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4530429 Mon, 14 Feb 2011 00:00:00 +0100 4530429 http://www.medworm.com/index.php?rid=4474612&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21310455%26dopt%3DAbstract Authors: Lee WI, Khim M, Im IR, Shin O, Park JW, Choo SJ, Yun TJ, Kim SW, Lee H Recently, human neonatal thymus-derived mesenchymal stromal cells (nTMSCs) have been recognized as a promising mesenchymal stem cell source for combined cell and gene therapy. While efficient gene transfer is crucial for optimizing therapeutic efficacy, almost no studies have yet reported on the characteristics of nTMSC in terms of genetic modification. The present study investigates and realizes the potential of self-complementary adeno-associated viruses (scAAVs) as an effective transduction tool for nTMSCs. Transduction efficiency (TE), cytotoxicity and functional characteristics were determined in nTMSCs isolated from thymic tissues and transduced with scAAV1-6 and -8 serotypes expressing GFP. Our study... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4474612 Tue, 08 Feb 2011 00:00:00 +0100 4474612 http://www.medworm.com/index.php?rid=4411785&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21255810%26dopt%3DAbstract In this study, we investigated the effect of transplantation of induced mouse embryonic stem cells (mESCs) into endoderm and early hepatocyte-like cells in streptozotocin (STZ)-diabetic mice. After embryoid body (EB) formation from mESC, the EBs were cultured in the presence of dexamethasone (DEX) and insulin for 4 days then was added acidic fibroblast growth factor (aFGF), hepatocyte growth factor (HGF) and oncostatin M (OSM) for 10 days, respectively. Blood glucose levels, intraperitoneal glucose tolerance (IGT) test and islet histology were assessed. The result revealed that transplantation of induced mESCs into early hepatocyte-like cells could repair pancreatic islets of control group. Blood glucose levels and intraperitoneal glucose tolerance test were significantly improved in test ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4411785 Wed, 19 Jan 2011 00:00:00 +0100 4411785 http://www.medworm.com/index.php?rid=4411783&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21255811%26dopt%3DAbstract Authors: Martins GF, Guedes BA, Silva LM, Serrão JE, Fortes-Dias CL, Ramalho-Ortigão JM, Pimenta PF Oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells with a smooth surface. Our results demonstrate that these cells remain viable in cell culture for at least two months. We also investigated their morphology in vivo and in vitro using light, confo... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4411783 Wed, 19 Jan 2011 00:00:00 +0100 4411783 http://www.medworm.com/index.php?rid=4411787&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21237473%26dopt%3DAbstract In conclusion, 10μM 5-Aza could promote the differentiation of c-kit+ cells into sinus node-like cells. 5-Aza-mediated differentiation seems to be helpful in future cell therapy for the patients suffering from loss of sinus node cells. PMID: 21237473 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4411787 Thu, 13 Jan 2011 00:00:00 +0100 4411787 http://www.medworm.com/index.php?rid=4344130&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21216421%26dopt%3DAbstract Authors: Insausti TC, Lazzari CR, Casas J The structural and ultrastructural characteristics of giant interneurons in the terminal abdominal ganglion of the cricket Nemobius sylvestris were investigated by means of cobalt and fluorescent dye backfilling and transmission electron microscopy. The projections of the 8 eight pairs of the biggest ascending interneurons (giant interneurons) are described in detail. The somata of all interneurons analyzed are located contralateral to their axons, which project to the posterior region of the terminal ganglion and arborise in the cercal glomerulus. Neuron 7-1a is an exception, because its arborisation is restricted to the anterior region of the ganglion. The fine structure of giant interneurons shows typical features of highly active cells. We ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4344130 Fri, 07 Jan 2011 00:00:00 +0100 4344130 http://www.medworm.com/index.php?rid=4344132&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21185580%26dopt%3DAbstract In conclusion, satureja essential oil significantly can ameliorate glomerular hypertrophy, loss of glomerular number, glomerulosclerosis and attenuated serum urea and serum creatinine in diabetic rats. PMID: 21185580 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4344132 Thu, 23 Dec 2010 00:00:00 +0100 4344132 http://www.medworm.com/index.php?rid=4344131&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21185581%26dopt%3DAbstract Authors: Smith SM, Melrose J The aim of this study was to determine if podoplanin was expressed by rudiment chondrocytes in human foetal cartilages. Podoplanin was immunolocalised in first trimester human foetal rib and knee joint rudiments to a sub-population of chondrocytes deep in the rib rudiments, tibial and femoral growth plates and cells associated with the cartilage canals of the foetal knee joint rudiments. Lymphatic vessels in the loose stromal tissues surrounding the developing rudiments were also demonstrated on the same histology slides using antipodoplanin (MAb D2-40) and anti-LYVE-1 and differentiated from CD-31 positive blood vessels confirming the discriminative capability of the antibody preparations used. The D2-40 positive rib and knee rudiment chondrocytes were not... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4344131 Thu, 23 Dec 2010 00:00:00 +0100 4344131 http://www.medworm.com/index.php?rid=4344133&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21185046%26dopt%3DAbstract Authors: Ba-Omar TA, Al-Jardani S, Victor R The changes in the behavior of Aphanius dispar, a cyprinodont fish when exposed to different concentrations of temephos, an organophosphorus pesticide widely used to control mosquito larvae in aquatic habitats are noted. The histopathology of gills exposed to temephos is studied in detail using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The gill lesions were seen in all exposed concentrations of 1, 5, 10 and 25ppm and the magnitude of the damage increased with the increase in concentrations. The most adverse effects of temephos are the destruction of cartilage and the large-scale cell death. Other effects included the degradation of chloride cells (CCs), desquamation, epithelial lifti... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4344133 Wed, 22 Dec 2010 00:00:00 +0100 4344133 http://www.medworm.com/index.php?rid=4344134&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176844%26dopt%3DAbstract Authors: Castellar A, Remedio RN, Barbosa RA, Gomes RJ, Caetano FH Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35mg/kg). Training program consisted of swimming 1h/day with a load of 4.8% of body weight for TD and 5.2% for TC, during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic aci... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4344134 Sun, 19 Dec 2010 00:00:00 +0100 4344134 http://www.medworm.com/index.php?rid=4279068&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21163506%26dopt%3DAbstract Authors: Quagio-Grassiotto I, Ortiz RJ, Pérez MH, Oliveira C Spermatic characteristics were studied in 10 species representing several distinct groups within the catfish family Doradidae. Interestingly, different types of spermatogenesis, spermiogenesis and spermatozoa are correlated with intrafamilial groups previously proposed for Doradidae. Semi-cystic spermatogenesis, modified Type III spermiogenesis, and biflagellate sperm appear to be unique within Doradidae to the subfamily Astrodoradinae. Other doradid species have sperm with a single flagellum, cystic spermatogenesis, and spermiogenesis of Type I (Pterodoras granulosus, Rhinodoras dorbignyi), Type I modified (Oxydoras kneri), or Type III (Trachydoras paraguayensis). Doradids have an external mode of fertilization, and share a... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4279068 Tue, 14 Dec 2010 00:00:00 +0100 4279068 http://www.medworm.com/index.php?rid=4202718&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21094964%26dopt%3DAbstract Authors: Pellegrini A, Bigliardi E, Bechi N, Paulesu L, Lehane MJ, Avanzati AM The female reproductive system of the tsetse fly Glossina morsitans morsitans is analysed by scanning electron microscopy (SEM). The study focuses in particular on the choriothete, a peculiar uterine structure involved in the viviparous mode of reproduction of Glossina morsitans morsitans. Under light microscopy, the choriothete appears formed by numerous tongue-like folds projecting towards the uterine lumen and lined by a thin cuticle. SEM analysis highlights for the first time a distinctive new feature that is not visible by traditional histological methods. That is a cuticular covering of the choriothete, which shows numerous thorns in the form of crest-like structures arranged in nearly parallel lines. ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4202718 Sat, 20 Nov 2010 00:00:00 +0100 4202718 http://www.medworm.com/index.php?rid=4202719&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21093886%26dopt%3DAbstract Authors: Gaino E, Scoccia F Fertile male polyps of three colonies of the black coral Rhipidipathes reticulata (Aphanipathidae) from Togian Islands (Indonesia) have been the source of the sperm investigated at ultrastructural level, in order to compare their organization with that of other members belonging to the family Antipathidae and Myriopathidae. The extension of the studies to a representative of the family Aphanipathidae stresses once more the structural similarity of the male gametes in antipatharians. A sketch of the sperm model reports the similarity and differences in the examined taxa. Among the micro-characters, the cup-like body linked to the pericentriolar apparatus, is exclusive of the antipatharians. Other inclusions concern the more common pro-acrosomal vesicles or th... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4202719 Thu, 18 Nov 2010 00:00:00 +0100 4202719 http://www.medworm.com/index.php?rid=4139959&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21051065%26dopt%3DAbstract Authors: Capkin E, Terzi E, Boran H, Yandi I, Altinok I Gill, trunk kidney, spleen, and liver of rainbow trout (Oncorhynchus mykiss) were examined after exposure to different sublethal concentrations of carbosulfan (25, 50 and 200μgL(-1)), propineb (3, 6 and 24mgL(-1)), and benomyl (2, 5 and 20mgL(-1)) for 14 days. Lesions were observed in gill, trunk kidney, spleen, and liver of rainbow trout exposed to either concentration of pesticides. The most important lesions were determined in the highest concentrations of pesticides. Lamellar fusion, lamellar hyperplasia, epithelial lifting, vacuolization of epithelial tissue, epithelial necrosis, hypertrophy and sloughing of epithelium were observed on fish exposed to carbosulfan, propineb and benomyl. Fish had cell necrosis, degeneration an... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4139959 Tue, 02 Nov 2010 00:00:00 +0100 4139959 http://www.medworm.com/index.php?rid=4139958&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21051066%26dopt%3DAbstract Authors: Talarico G, Michalik P The ultrastructure of spermatozoa is a valuable tool for phylogenetic and systematic studies. Ricinulei are enigmatic and poorly studied arachnids. So far, spermatozoa are only known from New World ricinuleids. The goals were to study, by means of light and transmission electron microcopy, the spermatozoa of an Old World species with regard to their phylogenetic implications, e.g., does the sperm structure contribute to the debated sister-group relationship of Acari and Ricinulei. The spermatozoa are coiled-flagellate and characterized by a cap-like acrosomal vacuole covered by electron-dense material, an elongated nucleus covered by a manchette of microtubules during spermiogenesis, an axoneme with a 9+2 microtubular pattern, a nuclear tube and axonemal... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4139958 Tue, 02 Nov 2010 00:00:00 +0100 4139958 http://www.medworm.com/index.php?rid=4139960&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21047663%26dopt%3DAbstract Authors: Ulivieri C An essential step in many forms of cell death is the release from mitochondria of "death effectors" which once in the cytoplasm activate signalling pathways leading to cellular demise. In this context mitochondria are known as regulators of cell death functioning as a node where signals are integrated. The discovery that alterations and remodelling of ultrastructural architecture of mitochondria are required to trigger the complete release of cytochrome c in the cytoplasm and the notion that mitochondrial architecture determines/influences the function of this organelle has fostered investigations on mitochondrial dynamics and on the machinery that regulates this process during cell death. In this review I shall summarize the current knowledge of mitochondrial inner... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4139960 Mon, 01 Nov 2010 00:00:00 +0100 4139960 http://www.medworm.com/index.php?rid=4108254&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20970817%26dopt%3DAbstract Authors: Chu C, Xu B, Huang W Studies indicate that many tissues could express follicle-stimulating hormone (FSH) besides pituitary. New functions of FSH are also been recognized beyond reproduction regulation. However, no report has been made about the expression and function of FSH in rat pancreas yet. Dual-labeled immunofluorescence stain, in situ hybridization and dual-labeled immunohistochemistry stain in adjacent sections were used to study the expression of FSH and its receptor, and co-localization of FSH with gonadotropin-releasing hormone (GnRH) receptor in rat pancreas. Tissue incubation and enzyme-linked immunosorbant assay (ELISA) were used to study the effects of FSH on the secretion of insulin and glucagon in rat pancreas in vitro. The results showed that rat pancreas cou... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4108254 Wed, 20 Oct 2010 23:00:00 +0100 4108254 http://www.medworm.com/index.php?rid=4108253&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20970818%26dopt%3DAbstract Authors: Sheng JF, Chen W, Yu Y, Liu J, Tao ZZ We investigated the relationship between telomerase activity and the expression of human telomerase reverse transcriptase (hTERT) and prostate apoptosis response 4 (Par-4) proteins in laryngocarcinoma cells. Following treatment with short hairpin RNA (shRNA) against hTERT, hTERT protein expression was inhibited, while Par-4 expression was increased. These changes were statistically significant (p<0.05), with Par-4 and hTERT expression being negatively correlated (p<0.05, r=-0.908). However, we found no correlation between telomerase activity and hTERT expression. Our findings suggest that hTERT exerts its anti-apoptotic effects through a telomerase-independent pathway, which is likely to intersect with the Par-4 apoptosis pathway. ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=4108253 Wed, 20 Oct 2010 23:00:00 +0100 4108253 http://www.medworm.com/index.php?rid=3973765&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20828773%26dopt%3DAbstract Authors: Lebelo SL, van der Horst G The ultrastructure of the Sertoli cell of the vervet monkey was studied using both scanning and transmission electron microscopic techniques. SEM micrographs revealed perforated sleeve-like processes which encased mature elongated spermatids which are ready for spermiation. TEM micrographs showed a large Sertoli cell nucleus characterized by many lobes (4-5) and consisting of a homogenous nucleoplasm and a distinctive nucleolus. The nucleus occupies a significant portion of the basal region of the cell. The distribution of chromatin clearly shows high activity of these cells. Lipid droplets and free ribosomes are also found scattered throughout the cytoplasm. Well-developed Golgi apparatus is found in the basal region of the cell. There is phagocytic... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3973765 Mon, 06 Sep 2010 23:00:00 +0100 3973765 http://www.medworm.com/index.php?rid=3973764&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20828774%26dopt%3DAbstract Authors: Moudilou EN, Mouterfi N, Exbrayat JM, Brun C Calpains are cytoplasmic proteases activated by calcium, implicated in cell differentiation and apoptosis. The best characterized enzymes are calpains 1-3. The aim of this work was to localize calpains 1-3 during the development of Xenopus laevis in order to clarify the function of these three proteases. For the first time, we detected the localization of the three proteases at the protein level between one-cell stage and adult age. Their expression was weak at early stages, then increased at tadpole stage and decreased through metamorphosis and adult life. The calpain's expression was maximal during the period characterized by the appearance of organs and modelling process. These observations suggest that calpains play a crucial ro... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3973764 Mon, 06 Sep 2010 23:00:00 +0100 3973764 http://www.medworm.com/index.php?rid=3956170&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817237%26dopt%3DAbstract Authors: Riccillo FL, Ronderos JR Allatotropin (AT) is a neuropeptide originally isolated from the brain of Manduca sexta and then characterized in several insect species. It acts as a neurohormone, as well as a neuromodulator. While it was primarily characterized on the basis of its ability to stimulate the secretion of juvenile hormones, it was also found that it acts as a cardioaccelerator and myostimulator. The presence of AT in IV instar larvae of T. infestans was previously described at the level of the Malpighian tubules. In the present study we report the presence of the peptide at the level of the brain, retrocerebral complex, as well as in the anterior midgut and aorta. The presence of AT at the corpora allata suggests that the peptide is acting on the gland during the first ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956170 Tue, 31 Aug 2010 23:00:00 +0100 3956170 http://www.medworm.com/index.php?rid=3956169&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817238%26dopt%3DAbstract CONCLUSION: Recombinant adeno-associated viral vectors co-expressing the hVEGF(165) and hBMP(7) genes showed efficient gene expression ability. The VEGF(165) and BMP(7) proteins expressed from the vector have efficient biological activity in vitro. PMID: 20817238 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956169 Tue, 31 Aug 2010 23:00:00 +0100 3956169 http://www.medworm.com/index.php?rid=3956168&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817239%26dopt%3DAbstract Authors: Rigon P, de Castilhos J, Molina CG, Zancan DM, Achaval M Nitric oxide (NO) is a gas produced through the action of nitric oxide synthase that acts as a neurotransmitter in the central nervous system (CNS) of adult gastropod mollusks. There are no known reports of the presence of NOS-containing neurons and glial cells in young and adult Megalobulimus abbreviatus. Therefore, NADPH-d histochemistry was employed to map the nitrergic distribution in the CNS of young and adult snails in an attempt to identify any transient enzymatic activity in the developing CNS. Reaction was observed in neurons and fibers in all CNS ganglia of both age groups, but in the pedal and cerebral ganglia, positive neurons were more intense than in other ganglia, forming clusters symmetrically located in ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956168 Tue, 31 Aug 2010 23:00:00 +0100 3956168 http://www.medworm.com/index.php?rid=3956167&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817240%26dopt%3DAbstract Authors: Sroyraya M, Chotwiwatthanakun C, Stewart MJ, Soonklang N, Kornthong N, Phoungpetchara I, Hanna PJ, Sobhon P The androgenic glands (AG) of male decapod crustaceans produce insulin-like androgenic gland (IAG) hormone that controls male sex differentiation, growth and behavior. Functions of the AG are inhibited by gonad-inhibiting hormone originating from X-organ-sinus gland complex in the eyestalk. The AG, and its interaction with the eyestalk, had not been studied in the blue swimmer crab, Portunus pelagicus, so we investigated the AG structure, and then changes of the AG and IAG-producing cells following eyestalk ablation. The AG of P. pelagicus is a small endrocrine organ ensheathed in a connective tissue and attached to the distal part of spermatic duct and ejaculatory bulb.... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956167 Tue, 31 Aug 2010 23:00:00 +0100 3956167 http://www.medworm.com/index.php?rid=3956158&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817241%26dopt%3DAbstract Authors: Muñoz L, Espinosa M, Quintanar-Jurado V, Hidalgo A, Melendez-Zajgla J, Maldonado V Multicellular spheroids are excellent models for the analysis of cancer behavior. Just like small avascular tumors, they present a marked zonal heterogeneity which influences gene expression and thus, growth and response to chemotherapy. In the present paper, we sought to analyze the effects of three-dimensional culture in the expression and distribution of estrogen receptor alpha. Using MCF-7 breast cancer cells, we found that multicellular spheroids in estrogen-containing medium presented a paradoxical regulation of estrogen receptor alpha, with a decrease in protein expression and a marked increase in mRNA steady-state levels. Immunohistochemistry showed that only sparse cells in the perip... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956158 Tue, 31 Aug 2010 23:00:00 +0100 3956158 http://www.medworm.com/index.php?rid=3956156&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817242%26dopt%3DAbstract In this study, the morphology of the VNO of the grass lizard Takydromus tachydromoides was examined using light and electron microscopy. The VNO consists of a pair of dome-shaped structures, which communicate with the oral cavity. There are no associated glandular structures. Microvilli are present on the apical surfaces of receptor cells in its sensory epithelium, as well as on supporting cells, and there are centrioles and ciliary precursor bodies on the dendrites. In addition to ciliated cells and basal cells in the non-sensory epithelium, there is a novel type of non-ciliated cell in T. tachydromoides. They have constricted apical cytoplasm and microvilli instead of cilia, and are sparsely distributed in the epithelium. Based on these results, the variation in the morphology of the VNO... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956156 Tue, 31 Aug 2010 23:00:00 +0100 3956156 http://www.medworm.com/index.php?rid=3956151&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817243%26dopt%3DAbstract Authors: Sacchi L, Genchi M, Clementi E, Negri I, Alma A, Ohler S, Sassera D, Bourtzis K, Bandi C Wolbachia is the most widespread bacterial endosymbiont in insects. It is responsible for a variety of reproductive alterations of the hosts. Wolbachia is transmitted through the germline from mother to offspring and, in rare cases, between individuals. This implies that acquired properties (through symbiosis with Wolbachia) can become heritable. We investigated the transovarial inheritance of Wolbachia in two phylogenetically distant insects, Drosophila melanogaster and Zyginidia pullula. We detected in both systems bacteriocyte-like cells, densely packed with Wolbachia endosymbionts, at the tip of the ovarioles. Bacteriocytes are cells specialized to harbour bacteria, typical of mutualis... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956151 Tue, 31 Aug 2010 23:00:00 +0100 3956151 http://www.medworm.com/index.php?rid=3956146&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817244%26dopt%3DAbstract Authors: Huang F, Yang YY, Shi M, Li JY, Chen ZQ, Chen FS, Chen XX The hemocytes of different types encountered in the diamondback moth Plutella xylostella larvae of each instar and the development of the differential hemocytes counts were herein presented. Hemocytes classes/populations characterized based on their affinity with fluorescent dye (acridine orange) and ultrastructural differences comprised the prohemcoytes (<10-16%), plasmatocytes (22-65%), granulocytes (25-72%), oenocytoids (<1-9%), and spherulocytes (<1%). Prohemcoytes were the smallest cells with a comparatively tremendous nucleus. Plasmatocytes and granulocytes occupied the main proportion of total cell numbers. Oenocytoids were in a most stable presence, i.e. rotund in a diameter of 10mum and with a nucleus ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3956146 Tue, 31 Aug 2010 23:00:00 +0100 3956146 http://www.medworm.com/index.php?rid=3934847&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20810141%26dopt%3DAbstract Authors: Kaloglu C, Onarlioglu B The endometrial extracellular matrix (ECM) remodelling has a crucial role in the establishment of a successful pregnancy. In addition to its basic function such as regulation of cell function, differentiation, migration, proliferation, the substantial alterations in the endometrial ECM may play a specific role in the trophoblast invasion, placentation, cell death and formation of the proper and functional implantation chamber around the embryo. In the present study, immunolocalizations of fibronectin and laminin were determined using avidin-biotin complex-peroxidase in rat implantation sites during 7-10 days of pregnancy. Both proteins were present in the basal membrane of blood vessels and in decidual matrix whereas they were absent or had very weak re... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3934847 Sun, 29 Aug 2010 23:00:00 +0100 3934847 http://www.medworm.com/index.php?rid=3934846&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20810142%26dopt%3DAbstract In conclusion, FD10GFs promotes the proliferation of chondrocytes and formation of good quality 'chondrocytes-fibrin' constructs which may have potential use of matrix-induced cell implantation. PMID: 20810142 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3934846 Sun, 29 Aug 2010 23:00:00 +0100 3934846 http://www.medworm.com/index.php?rid=3795553&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20655561%26dopt%3DAbstract In this study, the histopathological and histochemical changes due to chronic usage of morphine sulphate in liver were assessed in rats with both light and electron microscopes. Twenty male albino rats (Rattus norvegicus) (130-150g) were included and divided into four groups. Normal saline (5ml) was given orally as placebo in the control group (N=5). Morphine groups (N=5) received morphine orally at a single dose of 5ml/kg/day for 10, 20 and 30 days (groups II, III and IV), respectively. Liver specimens from all groups were evaluated for histopathological and histochemical changes. Light microscopy revealed severe centrilobular congestion, portal fibrosis with bile ductal proliferation and an increased inflammatory infiltration and focal parenchymal necrosis. Histochemical study revealed a... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3795553 Wed, 21 Jul 2010 23:00:00 +0100 3795553 http://www.medworm.com/index.php?rid=3663129&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20542530%26dopt%3DAbstract Authors: Paccagnini E, Mercati D, Giusti F, Conti B, Dallai R Spermatogenesis and the sperm structure of the terebrantian Aeolothrips intermedius Bagnall are described. Spermatogenesis consists of two mitotic divisions; the second is characterized by the loss of half of the spermatids, which have pyknotic nuclei. Early spermatids have two centrioles, but when spermiogenesis starts, a third centriole is produced. The three basal bodies give rise to three flagella; later these fuse into a single flagellum which contains three 9+0 axonemes. The basal bodies are surrounded by a large amount of centriole adjunct material. During spermiogenesis this material contributes to the shifting of the three axonemes towards the anterior sperm region parallel to the elongating nucleus, and it is trans... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3663129 Sun, 06 Jun 2010 23:00:00 +0100 3663129 http://www.medworm.com/index.php?rid=3663128&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20542531%26dopt%3DAbstract Authors: Vergallo C, Fonseca T, Pizzi G, Dini L The maintenance of a healthy corneal epithelium under both normal and wound healing conditions is achieved by a population of stem cells (SCs) located in the basal epithelium at the corneoscleral limbus. In the light of the development of strategies for reconstruction of the ocular surface in patients with limbal stem cell deficiency, a major challenge in corneal SCs biology remains the ability to identify stem cells in situ and in vitro. To date, not so much markers exist for the identification of different phenotypes. CESCs (corneal epithelial stem cells) isolated from limbal biopsies were maintained in primary culture for 14 days and stained with Hoechst and a panel of FITC-conjugated lectins. All lectins, with the exception of Lycoper... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3663128 Sun, 06 Jun 2010 23:00:00 +0100 3663128 http://www.medworm.com/index.php?rid=3600232&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20493508%26dopt%3DAbstract Authors: Che TC, François S, Bouchet S, Chapel A, Forgue-Lafitte ME The development of ACF (aberrant crypt foci), adenoma and cancer following intrarectal administration of the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) has been described. However, microscopic lesions not previously reported were observed as soon as two weeks following carcinogen treatment. These lesions protrude slightly over the epithelial lining of the colon, with a micropolyp-like appearance. Oriented sections show that the centre of these lesions present pseudo-"cystic" appearance, with disorganized crypts made of normal cells. The chorion of the lesion is invaded by numerous inflammatory cells and some ACF may be present nearby. The epithelium lining the cysts and the distorted crypts shows e... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3600232 Tue, 18 May 2010 23:00:00 +0100 3600232 http://www.medworm.com/index.php?rid=3582443&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20483444%26dopt%3DAbstract In conclusion, adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for the future cell replacement therapy. PMID: 20483444 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3582443 Sun, 16 May 2010 23:00:00 +0100 3582443 http://www.medworm.com/index.php?rid=3532294&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20434188%26dopt%3DAbstract Authors: Ghaedi M, Lotfi AS, Soleimani M Alpha-1 antitrypsin (AAT) deficiency is a lethal hereditary disorder characterized by a severe diminution in plasma levels of AAT leading to progressive liver dysfunction. Since mesenchymal stem cells can differentiate into hepatocyte-like cells they offer a potential unlimited source in autologous transplant procedures. The transfer of genetically modified hepatocyte cells derived from hMSCs into the body constitutes a novel paradigm of coupling cell therapy with gene therapy for this disease. hMSCs were isolated by density gradient centrifugation and plastic adherence. Hepatic differentiation was induced by exposing hMSC to induction medium for up to 21 days. The mRNA levels and protein expression of several important hepatic genes were determ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3532294 Wed, 28 Apr 2010 23:00:00 +0100 3532294 http://www.medworm.com/index.php?rid=3532289&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20434749%26dopt%3DAbstract In this study the long-term effects of morphine sulphate treatment (MST) on histopathological and biochemical changes in the cerebellum was assessed in albino rats. Normal saline (5ml) was given orally as placebo in the control group (n=25). Morphine groups received morphine orally at a dose level of 5mg/kg body weight day after day for 10, 20 and 30 days (n=25/group). Light microscopy revealed that the molecular layer showed vacuolation. The Purkinje cells lost their specific shaped appearance, decreased in size and numbers. The granular cells highly degenerated. Electron microscopy revealed fragmentation of the cisterns of the both types of endoplasmic reticulum, resulted in a progressive depletion of total protein contents as well as general carbohydrates in all treated groups as suppor... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3532289 Wed, 28 Apr 2010 23:00:00 +0100 3532289 http://www.medworm.com/index.php?rid=3532290&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20434189%26dopt%3DAbstract In this study, we examined 7B2 (secretogranin V)-like immunoreactivity (IR) in the Japanese red-bellied newt (Cynops pyrrhogaster) pituitary. Results showed that the pars nervosa was filled with immunoreactive granules. In the pars intermedia, all melanotrophs showed 7B2-IR. In the pars distalis, immunoreactive cells were dispersed, and the 7B2-immunoreactive cells were also immunopositive for the beta-subunit of bullfrog luteinizing hormone (fLHbeta). 7B2-IR co-localized with fLHbeta-IR in the same secretory granules. Our results suggest that 7B2 may participate in the secretion processes of gonadotropins in the pars distalis. PMID: 20434189 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3532290 Tue, 27 Apr 2010 23:00:00 +0100 3532290 http://www.medworm.com/index.php?rid=3522933&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20430411%26dopt%3DAbstract Authors: Boran H, Altinok I, Capkin E Acute toxicity of the pesticides, maneb and carbaryl, to juvenile rainbow trout were evaluated under static-renewal test conditions. Actual concentrations of maneb ranged from 0.10mg/L to 2.00mg/L and carbaryl ranged from 0.20mg/L to 3.90mg/L. The concentrations of maneb that killed 50% of the rainbow trout (3.27+/-0.9g) within 24-h (24-h; LC(50)), 48-h, 72-h and 96-h were 1.19+/-0.12, 1.04+/-0.11, 0.92+/-0.12 and 0.81+/-0.14mg/L (95% confidence limits), respectively. LC(50) values of carbaryl for 24-h, 48-h, 72-h and 96-h were 2.52+/-0.71, 2.16+/-0.63, 1.71+/-0.46 and 1.39+/-0.15mg/L, respectively. None of the unexposed control fish died and the first fish died 6h after exposure to maneb (>/=1.30mg/L), and carbaryl (>/=2.60mg/L). Lamellar ed... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3522933 Mon, 26 Apr 2010 23:00:00 +0100 3522933 http://www.medworm.com/index.php?rid=3522934&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20427066%26dopt%3DAbstract CONCLUSION: The co-culture perfusion system is a new tool to investigate interactions of different cell types with less artificial interferences. Our results suggest that synovial supernatants and synovial fibroblasts modulate the biosynthetic activity and the matrix deposition of chondrocytes as well as the susceptibility to radical attack of reactive oxygen species. PMID: 20427066 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3522934 Sun, 25 Apr 2010 23:00:00 +0100 3522934 http://www.medworm.com/index.php?rid=3409044&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20334886%26dopt%3DAbstract In this study, we analyzed the expression of Tnmd, Chm1, alpha 1 chain of the type I collagen (Col1a1) and alpha 1 chain of the type II collagen (Col2a1) at the insertion site of the Achilles, patellar, or rotator cuff tendons of 1-week-old rabbits by in situ hybridization analysis. Tnmd was co-expressed with Col1a1 in tenocytes of these tendons, while Chm1 and Col2a1 were detected in chondrocytes of the hyaline cartilage. Interestingly, the cell population between Tnmd/Col1a1 positive tenocytes and Chm1/Col2a1 positive chondrocytes expressed Col1a1 but none of the other markers (Tnmd, Chm1, and Col2a1). Red blood cells were exclusively present at the interface between the tendon substance and cartilage in the insertion site of the Achilles tendon. Lack of Tnmd and Chm1 in this newly chara... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3409044 Mon, 22 Mar 2010 00:00:00 +0100 3409044 http://www.medworm.com/index.php?rid=3400049&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20307898%26dopt%3DAbstract Authors: Vodenicharov A, Bozhilova-Pastirova A The enzymehistochemical localization of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) reactive cells next and within the autonomic nerves in the porcine kidney's hilus, close to renal artery and vein as well as in its adventitia, was studied. It was established that the prevailing part of NADPH-d-reactive cells were distributed next to and within NADPH-d-reactive autonomic fibers around the renal vein. Comparatively small number of reactive cells was found around the renal artery, but single NADPH-d-reactive cells were found in the neighbouring autonomic nerves as well. The cells observed next to nerves had much more expressed reaction (granules) than those, located in the nerves. Toluidine blue staining on frozen and pa... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3400049 Sat, 20 Mar 2010 00:00:00 +0100 3400049 http://www.medworm.com/index.php?rid=3391381&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20304454%26dopt%3DAbstract Authors: Alam MS, Andrina BB, Tay TW, Tsunekawa N, Kanai Y, Kurohmaru M Morphological alterations in seminiferous tubules caused by single administration of di(n-butyl) phthalate (DBP) in 3-week-old rats were investigated throughout the first wave of spermatogenesis. Single administration of DBP (500mg/kg) showed progressive detachment and displacement of spermatogenic cells and disappearance of tubular lumen at 3h after treatment, and then showed thin seminiferous epithelia and wide tubular lumen at day 1 (D1). At D1, quite significant numbers of apoptotic spermatogenic cells were detected, and then they gradually decreased in accordance with the passage of time. In contrast, the testes revealed lower weight gain, even after completion of first wave of spermatogenesis in the DBP-treat... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3391381 Fri, 19 Mar 2010 00:00:00 +0100 3391381 http://www.medworm.com/index.php?rid=3391382&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20303561%26dopt%3DAbstract Authors: Chen KS, Tatarczuch L, Ahmed Y, Huang HH, Mirams M, Pagel CN, Mackie EJ Hypertrophic "light" and "dark" chondrocytes have been reported as morphologically distinct cell types in growth cartilage during endochondral ossification in many species, but functional differences between the two cell types have not been described. The aim of the current study was to develop a pellet culture system using chondrocytes isolated from epiphyseal cartilage of neonatal mice and rats, for the study of functional differences between these two cell types. Hypertrophic chondrocytes resembling those described in vivo were observed by light and electron microscopy in sections of pellets treated with triiodothyronine, 1% fetal calf or mouse serum, 10% fetal calf serum or 1.7MPa centrifugal pressure ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3391382 Thu, 18 Mar 2010 00:00:00 +0100 3391382 http://www.medworm.com/index.php?rid=3347406&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20206955%26dopt%3DAbstract Authors: Pinchuck SC, Hodgson AN Histology and electron microscopy were used to describe and compare the structure of the perinotal epidermis and defensive glands of two species of shell-less marine Systellommatophora, Onchidella capensis and Onchidella hildae (Onchidiidae). The notum of both species is composed of a layer of epithelial and goblet cells covered by a multi-layered cuticle. Large perinotal multi-cellular glands, that produce thick white sticky mucus when irritated, are located within the sub-epidermal tissue. The glands are composed of several types of large secretory cell filled with products that stain for acidic, sulphated and neutral mucins, and some irregularly shaped support cells that surround a central lumen. The products of the secretory cells are produced by or... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3347406 Thu, 04 Mar 2010 00:00:00 +0100 3347406 http://www.medworm.com/index.php?rid=3303369&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20171710%26dopt%3DAbstract Authors: Riparbelli MG, Dallai R, Callaini G Centrioles are involved in the formation of cilia and flagella and in the organization of the centrosome, the main microtubule-organizing center of the cell. Since structural and functional centriole aberrations can contribute to several human diseases, these organelles have recently focused the attention of cell biologists. Here, we review data on the insect centrioles, reporting non-canonical aspects of their behaviour, function and architecture. These findings that represent obvious exceptions to the established rule might contribute to advance our understanding in the formation and the function of centrioles. PMID: 20171710 [PubMed - as supplied by publisher] (Source: Tissue and Cell) Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3303369 Thu, 18 Feb 2010 00:00:00 +0100 3303369 http://www.medworm.com/index.php?rid=3263338&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20144467%26dopt%3DAbstract Authors: Dallai R, Mercati D, Bu Y, Yin YW The general organization of the male genital system, the spermatogenesis and the sperm structure of the proturan Acerella muscorum have been described. At the apex of testis apical huge cells are present; their cytoplasm contains a conventional centriole, a large amount of dense material and several less electron-dense masses surrounded by mitochondria. Spermatocytes have normal centrioles and are interconnected by cytoplasmic bridges. Such bridges seem to be absent between spermatid cells and justify the lack of synchronization of cell maturation. Spermatids are almost globular cells with a spheroidal nucleus and a large mass of dense material corresponding to the centriole adjunct. Within this mass a centriole is preserved. Mitochondria of n... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3263338 Sat, 06 Feb 2010 00:00:00 +0100 3263338 http://www.medworm.com/index.php?rid=3164626&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20060993%26dopt%3DAbstract Fine structure of spermatozoa in the blackspot sea bream Pagellus bogaraveo (Brünnich, 1768) with some considerations about the centriolar complex. Tissue Cell. 2010 Jan 8; Authors: Maricchiolo G, Laurà R, Genovese L, Guerrera MC, Micale V, Muglia U Scanning and transmission electron microscopy were used to investigate the fine structure of the sperm of the sparid fish Pagellus bogaraveo. The spermatozoon of P. bogaraveo belongs, like that of the other sparid fish, to the teleostean "type I" spermatozoon with the flagellar axis insert perpendicular to the nuclear fossa. It has an ovoidal head, a short, cylindrically shaped midpiece and a long tail region. The nucleus reveals a deep invagination (nuclear fossa), in which the centriolar complex is located, and a satelli... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3164626 Fri, 08 Jan 2010 00:00:00 +0100 3164626 http://www.medworm.com/index.php?rid=3164625&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20060994%26dopt%3DAbstract Authors: Zizzari ZV, Machida R, Tsutsumi K, Reynoso-Velasco D, Lupetti P, Dallai R Previous studies have demonstrated the presence of sperm dimorphism in the Mantispidae Perlamantispa perla. We extended the study on several other mantidflies. In all the examined species the occurrence of euspermatozoa (typical) and paraspermatozoa (atypical) was established. The euspermatozoa are characterized by the presence of a cylindrical nucleus surrounded by an envelope that fans out laterally into two thin wings of different length. The acrosome seems to be missing. The nucleus is surrounded by extracellular material. The flagellum is provided with a 9+9+2 axonemal pattern; the accessory tubules contain 16 protofilaments and the intertubular material has the distribution typical of the taxon. Tw... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=3164625 Fri, 08 Jan 2010 00:00:00 +0100 3164625 http://www.medworm.com/index.php?rid=2928033&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19853267%26dopt%3DAbstract Authors: Wang JX, Peng KM, Liu HZ, Song H, Chen X, Min L We used light microscopy to elucidate the morphological features of argyrophilic cells in the digestive tract of the African ostrich (Struthio camelus). The results indicated that argyrophilic cells were found to be distributed among the epithelial cells of the mucosa or glands throughout the digestive tract, except for the esophagus; two types of argyrophilic cells were found; i.e., closed-type cells and cells with triangular or elongated shapes and with their apical cytoplasmic process in contact with the lumen (open-type cells); the greatest number of argyrophilic cells was found in the proventriculus, and the argyrophilic cell density gradually decreased from the proventriculus to the rectum; Furthermore, the number of argyro... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2928033 Wed, 21 Oct 2009 00:00:00 +0100 2928033 http://www.medworm.com/index.php?rid=2902262&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19833367%26dopt%3DAbstract Authors: Rigon F, Mânica G, Guma F, Achaval M, Faccioni-Heuser MC In Megalobulimus abbreviatus, the ultrastructural features and the contractile proteins of columellar, pharyngeal and foot retractor muscles were studied. These muscles are formed from muscular fascicles distributed in different planes that are separated by connective tissue rich in collagen fibrils. These cells contain thick and thin filaments, the latter being attached to dense bodies, lysosomes, sarcoplasmic reticulum, caveolae, mitochondria and glycogen granules. Three types of muscle cells were distinguished: T1 cells displayed the largest amount of glycogen and an intermediate number of mitochondria, suggesting the highest anaerobic metabolism; T2 cells had the largest number of mitochondria and less glycogen,... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2902262 Mon, 12 Oct 2009 23:00:00 +0100 2902262 http://www.medworm.com/index.php?rid=2902261&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19833368%26dopt%3DAbstract Authors: Leknes IL Histochemical properties of intestine goblet cells in firemouth cichlid, zebra mbuna, freshwater angelfish and platyfish are described. Goblet cells occurred regularly in the epithelial cell layer throughout the entire intestine, they were strongly coloured by alcian blue at pH 2.5. This colour got gradually weaker when the pH was reduced, but still after alcian blue at pH 0.2 these cells displayed a distinct blue colour. When the goblet cells were treated with periodic acid-Schiff (PAS), they displayed a strong purple-magenta colour. The findings that a number of goblet cells displayed various colours between blue and purple-magenta when acidic alcian blue was followed by PAS, and between blue and red-brown when acidic alcian blue was followed by neutral red, may re... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2902261 Mon, 12 Oct 2009 23:00:00 +0100 2902261 http://www.medworm.com/index.php?rid=2768714&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19726067%26dopt%3DAbstract Authors: Cieniewicz AM, Woodruff RI In fish, amphibians and mammals, gap junctions of some cells allow passage of elongate molecules as large as 18kDa, while excluding smaller, less elongate molecules. Fluorescently labeled Calmodulin (17kDa) and fluorescently labeled Troponin-C (18kDa), when microinjected into oocytes of Danio rerio, Xenopus laevis or Mus domestica, were able to transit the gap junctions between these oocytes and the granulosa cells which surrounded them. Co-microinjected with these Ca(2+)-binding proteins, Texas-red-labeled dextran (10kDa) remained in the microinjected cell. Osteocalcin (6kDa), also a Ca(2+)-binding protein, but with a wide "V" shape proved unable to transit these gap junctions. Calmodulin, but not Troponin-C, was able to transit gap junctions of gon... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2768714 Sun, 30 Aug 2009 23:00:00 +0100 2768714 http://www.medworm.com/index.php?rid=2742273&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19709709%26dopt%3DAbstract Authors: Steinbacher P, Tauber M, Kogler S, Stoiber W, Resch H, Sänger AM Ruptures of the rotator cuff tendons of the human shoulder are a common incidence and lead to functional impairment of the four muscles connected to the cuff, entailing profound changes of their cellular tissue composition. Most importantly, such tendon tears lead to atrophy, fatty degeneration and fibrosis of the corresponding muscles. The muscle most commonly affected with such changes is the M. supraspinatus. The present study uses biopsy samples from the supraspinatus muscle of 12 elderly patients and 6 controls to examine the rupture-induced muscle change at both the cellular and the intracellular (ultrastructural) levels. Amounts of fatty tissue, connective tissue and muscle were assessed by light micr... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2742273 Sun, 23 Aug 2009 23:00:00 +0100 2742273 http://www.medworm.com/index.php?rid=2692282&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19665160%26dopt%3DAbstract Authors: Pan MH, Cai XJ, Liu M, Lv J, Tang H, Tan J, Lu C A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green f... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2692282 Wed, 05 Aug 2009 23:00:00 +0100 2692282 http://www.medworm.com/index.php?rid=2675497&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19651422%26dopt%3DAbstract Authors: Hua J, Yu H, Dong W, Yang C, Gao Z, Lei A, Sun Y, Pan S, Wu Y, Dou Z Pluripotent mesenchymal stem-like cell lines were established from lungs of 3-4 months old aborted fetus. The cells present the high ex vivo expansion potential of MSC, a typical fibroblast-like morphology and proliferate up to 15 passages without displaying clear changes in morphology. Immunological localization and flow cytometry analyses showed that these cells are positive for OCT4, c-Kit, CD11, CD29, CD44, telomerase, CD106, CD105, CD166, and SSEA1, weakly expression or negative for SSEA1, SSEA3, SSEA4, CD34, CD105 and CD106. These cells can give rise to the adipogenic as evidenced by accumulation of lipid-rich vacuoles within cells identified by Oil-red O when they were induced with 0.5mM isobutylmethyl... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2675497 Fri, 31 Jul 2009 23:00:00 +0100 2675497 http://www.medworm.com/index.php?rid=2661078&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19643451%26dopt%3DAbstract Authors: Michalik P, Knoflach B, Thaler K, Alberti G Monogyny in spiders culminates in extreme traits, like dramatic male self-sacrifice and emasculation of the male by the female during copulation. Here we show that monogynous males can be highly adapted for this fatal sexual behaviour. Dwarf males of the one-palped theridiid spider Tidarren argo, which are cannibalised immediately after the insertion of their single copulatory organ, stop spermiogenesis when reaching adulthood. Their testes atrophy, which might economise the energy expenditures of these males. We also found that the amount of seminal fluid produced is stored in an enlarged seminal vesicle until the single sperm induction takes place. The volume of the seminal vesicle is similar to the sperm droplet taken up into the ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2661078 Mon, 27 Jul 2009 23:00:00 +0100 2661078 http://www.medworm.com/index.php?rid=2661077&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19643452%26dopt%3DAbstract Authors: Rost-Roszkowska MM, Machida R, Fukui M Midgut epithelium in Filientomon takanawanum is composed of epithelial cells and single, sporadic regenerative cells. In 80% of analyzed specimens midgut epithelial cells, as fat body and gonads, are infected with rickettsia-like microorganism. In non-infected specimens young and completely differentiated epithelial cells are distinguished among epithelial cells. Characteristic for midgut epithelial cells regionalization in organelles distribution is not observed. Autophagy is the sporadic process, but if the cytoplasm of epithelium cells possesses numerous spherites and sporadic autophagosomes, the apoptosis begins. Necrosis is observed sporadically. In the midgut epithelium cells of about 80% of analyzed specimens rickettsia-like microo... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2661077 Mon, 27 Jul 2009 23:00:00 +0100 2661077 http://www.medworm.com/index.php?rid=2661079&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19640554%26dopt%3DAbstract Authors: Hosaka YZ, Takahashi H, Uratsuji T, Tangkawattana P, Ueda H, Takehana K The aim of this study was to determine the characteristic differences in tendinocytes derived from tendons in the equine forelimb, superficial digital flexor tendon (SDFT), deep digital flexor tendon (DDFT) and common digital extensor tendon (CDET), in morphology, proliferation, collagen production ability and ability for synthesis of matrix metalloproteinases (MMPs). Significant differences were observed in cell number in vivo. The cellular number was largest in the SDFT and smallest in the CDET. The values of in vitro proliferation ratios and ability for synthesis of collagen and MMPs were largest in the SDFT and smallest in the CDET. Addition of TNFalpha to culture of all three types of tendinocytes inc... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2661079 Sun, 26 Jul 2009 23:00:00 +0100 2661079 http://www.medworm.com/index.php?rid=2646403&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19632698%26dopt%3DAbstract Authors: Sottile L, Brundo MV, Viscuso R In the male genital tract of Tettigoniidae, the spermatodesms are composed of a limited number of spermatozoa whose nuclei and acrosomes are covered by a mucous cap. The formation of the cap begins in the testicular cyst during the lengthening of the apical prolongations of the spermatids and the spermatids' simultaneous division into small bundles or spermatodesms. The cap material is formed from a loosely arranged material in the lumen of the cyst, probably produced by the secretory activity of the delimiting cells. Another characteristic aspect of the Tettigoniidae is the rearrangement of the cap inside the spermiduct that seems to start when material from the lumen of the organ enters from the basal part of the cap. Except for the fibrils of... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646403 Thu, 23 Jul 2009 23:00:00 +0100 2646403 http://www.medworm.com/index.php?rid=2646405&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631953%26dopt%3DAbstract Authors: Tinikul Y, Mercier AJ, Sobhon P Dopamine (DA), octopamine (OA) and serotonin (5-HT) are the key neurotransmitters that control gonadal development in decapod crustaceans. 5-HT stimulates, while DA and OA delay gonadal development in Macrobrachium rosenbergii. In the present study, we have further investigated the distribution patterns of DA and OA in the central nervous system (CNS) and ovary during various stages of the ovarian maturation cycle of this giant freshwater prawn. DA- and OA-immunoreactive neurons and fibers were distributed extensively in several regions of the brain, subesophageal ganglion (SEG), thoracic ganglia and abdominal ganglia. In the brain, the two neurotransmitters were present in neurons of clusters 6, 7, 11, 17, and nearby neuropil regions. In the SE... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646405 Wed, 22 Jul 2009 23:00:00 +0100 2646405 http://www.medworm.com/index.php?rid=2646404&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631954%26dopt%3DAbstract Authors: Pozuelo JM, Rodríguez R, Arriazu R, Ingelmo I, Martín R, Santamaría L The amount of neurons of periprostatic accessory ganglia in pre- and peripubertal rats was studied to ascertain whether the development of these autonomic ganglia is androgen-dependent. Stereological estimates of the volumes and number of neurons immunoreactive to protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), and vasoactive intestinal polypeptide (VIP) were carried out. Immunostaining of androgen receptors (AR) in the ganglia was also performed. The ganglionic neurons from the two groups studied were immunoreactive to PGP 9.5, NPY, and VIP. Almost all the neurons were immunostained for AR. The ganglionic volume showed a significant increase in peripubertal prostate in comparison wi... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646404 Wed, 22 Jul 2009 23:00:00 +0100 2646404 http://www.medworm.com/index.php?rid=2646409&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631356%26dopt%3DAbstract In this study we attempted to evaluate overall proliferation rates in general, as well as foam cells and the endothelial cells lining newly formed plaque microvessels in particular. For this purpose, cellular proliferation was assessed through immunohistochemical staining for PCNA in 10 fresh human carotid artery samples received from patients undergoing carotid endarterectomy. Overall proliferative activity was found significantly higher (P</=0.01) among complicated type VI lesions compared to uncomplicated type V lesions. A similar assessment focused on foam cells alone also revealed a significantly higher (P</=0.05) proliferative index among complicated lesions. On the other hand, the proliferation rate for the endothelial cells lining the interior walls of newly formed microvesse... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646409 Tue, 21 Jul 2009 23:00:00 +0100 2646409 http://www.medworm.com/index.php?rid=2646408&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631357%26dopt%3DAbstract Authors: Insausti TC, Casas J Ommochromes are end products of the tryptophan metabolism in arthropods. While the anabolism of ommochromes has been well studied, the catabolism is totally unknown. In order to study it, we used the crab-spider Misumena vatia, which is able to change color reversibly in a few days, from yellow to white and back. Ommochromes is the only pigment class responsible for the body coloration in this animal. The aim of this study was to analyze the fine structure of the epidermal cells in bleaching spiders, in an attempt to correlate morphological changes with the fate of the pigment granules. Central to the process of bleaching is the lysis of the ommochrome granules. In the same cell, intact granules and granules in different degradation stages are found. The d... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646408 Tue, 21 Jul 2009 23:00:00 +0100 2646408 http://www.medworm.com/index.php?rid=2646407&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631358%26dopt%3DAbstract Authors: Jin YG, Park JJ, An CM, Lee JS The ovipositor of striped bitterling Acheilognathus yamatsutae was subjected to ultrastructure and histochemical analysis during spawning season using light and electron microscopy. Although the ovipositor of A. yamatsutae is a long cylindrical tube with smooth external surface, it was possible to confirm the presence of well-developed fingerprint structure using scanning electron microscopy. Internal aspect analysis of ovipositor revealed formation of 5-8 longitudinal folds. Cross section analysis revealed that the ovipositor is composed of an outer epithelial layer, a mid connective tissue layer, and an inner epithelial layer. The outer epithelial layer contains 7-9 cell layers composed mainly of epithelial and mucous cells. Result of AB-PAS (p... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646407 Tue, 21 Jul 2009 23:00:00 +0100 2646407 http://www.medworm.com/index.php?rid=2646406&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19631359%26dopt%3DAbstract Authors: Haberstroh K, Enz A, Zenclussen ML, Hegewald AA, Neumann K, Abbushi A, Thomé C, Sittinger M, Endres M, Kaps C The aims of this work were to test whether human intervertebral disc-derived nucleus pulposus cells (hNP-cells) are attracted by human serum and to analyze if matrix generation from hNP-cells is promoted under the influence of transforming growth factor-beta3 (TGF-beta3) or hyaluronan (HA) in vitro. Using the multi-well chemotaxis assay to determine cell migration under the influence of different concentrations of human serum, it was demonstrated that dedifferentiated hNP-cells are able to migrate towards a serum fraction gradient in a concentration-dependent manner. Re-differentiation capacity of hNP-cells in 3D micro-masses under the influence of TGF-beta3 or hy... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2646406 Tue, 21 Jul 2009 23:00:00 +0100 2646406 http://www.medworm.com/index.php?rid=2526878&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19524276%26dopt%3DAbstract Authors: Li W, Ma L, Zhao J, Liu X, Li Z, Zhang Y MTA1, as a constituent of the nucleosome-remodeling and -deacetylation complex (NuRD), is thought to modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of several cancer cell lines and tissues, MTA1 can also regulate divergent cellular pathways by modifying the acetylation status of crucial target genes. However, its fundamental physiological functions have not been characterized. To further address the possible physiological role of this protein in mammals, the authors examined the expression pattern of mouse MTA1 in a variety of adult mouse tissues by a combination of techniques, including semi-quantitative RT-PCR, Western blotting and immunohistochemi... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2526878 Wed, 10 Jun 2009 23:00:00 +0100 2526878 http://www.medworm.com/index.php?rid=2526874&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19524277%26dopt%3DAbstract Authors: Di Bella MA, Carbone MC, D'Amato M, Alessandro R, De Leo G The intermediate filament (IF) proteins Styela C and Styela D from the tunicate Styela (Urochordata) are co-expressed in all epidermal cells and they are thought to behave as type I and type II keratins. These two IF proteins, Styela C and Styela D, were identified in immunoblots of proteins isolated from the tunic of Styela plicata. The occurrence and distribution of these proteins within the tunic of this ascidian was examined by means of immunofluorescence and immunoperoxidase techniques, using anti-Styela C and anti-Styela D antibodies. In addition, immuno-electron microscopy of the tunic showed that the two proteins are located in the cuticle layer and in the tunic matrix. These results represent the first data ab... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2526874 Wed, 10 Jun 2009 23:00:00 +0100 2526874 http://www.medworm.com/index.php?rid=2526885&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19406448%26dopt%3DAbstract Authors: Milanesi C, Vignani R, Ciacci A, Nardini A, Valenti M, Cantini F, Ciampolini F, Faleri C, Cresti M The ultrastructure of Vitis vinifera seeds from different archaeological sites was studied. Preservation status differed between sites. Preliminary investigations of grape seeds from Poggio Bacherina (Chianciano Terme, Siena) and Miranduolo (Chiusdino, Siena) showed collapsed or charred tegument, making this material suitable for morphometric studies only. Rapid-freeze fixation and substitution of grape seeds from Shahr-I Sokhta in Iran and via De' Castellani in Florence revealed well preserved tegument suitable for chemical and cytochemical analysis. Energy dispersive X-ray microanalysis was used to determine chemical composition. Cytochemical analysis based on fluorescent stain... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2526885 Mon, 27 Apr 2009 23:00:00 +0100 2526885 http://www.medworm.com/index.php?rid=2526892&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19362728%26dopt%3DAbstract In this study, the salivary glands of the carnivorous cephalaspidean Philinopsis depicta were observed by light and electron microscopy. The secretory epithelium of these ribbon-shaped glands is formed by ciliated cells, granular cells and cells with apical vacuole. In ciliated cells the nucleus and most cytoplasmic organelles are located in the wider apical region and a very thin stalk reaches the base of the epithelium. These cells possess significant amounts of glycogen. Granular cells are packed with electron-dense secretory granules and also contain several cisternae of rough endoplasmic reticulum and Golgi stacks. The other type of secretory cell is mainly characterized by the presence of a large apical vacuole containing secretion. These cells possess high amounts of rough endoplasm... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2526892 Thu, 09 Apr 2009 23:00:00 +0100 2526892 http://www.medworm.com/index.php?rid=2301143&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19342069%26dopt%3DAbstract Authors: Simeó CG, Ribes E, Rotllant G The morphology and function of the male reproductive system in the spider crab Maja brachydactyla, an important commercial species, is described using light and electron microscopy. The reproductive system follows the pattern found among brachyuran with several peculiarities. The testis, known as tubular testis, consists of a single, highly coiled seminiferous tubule divided all along by an inner epithelium into germinal, transformation, and evacuation zones, each playing a different role during spermatogenesis. The vas deferens (VD) presents diverticula increasing in number and size towards the median VD, where spermatophores are stored. The inner monostratified epithelium exocytoses the materials involved in the spermatophore wall formation... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2301143 Tue, 31 Mar 2009 04:00:00 +0100 2301143 http://www.medworm.com/index.php?rid=2301147&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19329135%26dopt%3DAbstract Authors: Zybina EV, Bogdanova MS, Stein GI, Vlasova TD, Zybina TG The supergiant trophoblast cells characteristic of vole placenta prove to be highly invasive being found at the boundary of the decidualized endometrium and myometrium. Their size (100mum and higher) suggests them to be highly polyploid, though their ploidy was not determined by now. We performed determination of the ploidy level of the supergiant trophoblast cells (SuGT) in order to verify whether the highly polyploid trophoblast cells are capable of deep intrauterine invasion. Anti-Cytokeratin trophoblast immunolabelling were performed to estimate the ways of the SuGT migration. DNA content measurement with help of image analysis was performed at the series of Feulgen-stained sections of the SuGT nuclei. The SuGT were ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2301147 Fri, 27 Mar 2009 04:00:00 +0100 2301147 http://www.medworm.com/index.php?rid=2301149&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19328511%26dopt%3DAbstract In this study, apoptosis was identified by TUNEL staining and then related to cell proliferation, as determined by Ki-67 staining. The apoptotic index was at its highest at 8 days of involution, whereas the proliferation index was at its highest during lactation. Caspase-3 was immunolocalised only in mast cells and along the basal membrane in the mammary tissue at -10 days from lambing, 150 days of lactation and at 8 days of involution. This finding could indicate that caspase-3 is not involved in sheep mammary gland apoptosis, but that other proteins - such as apoptosis inducing factor (AIF) - can trigger apoptosis, through the mitochondrial pathway, in a caspase-independent manner. The expression of genes involved in the regulation of lactation and apoptosis was also investigated and det... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2301149 Thu, 26 Mar 2009 04:00:00 +0100 2301149 http://www.medworm.com/index.php?rid=2301153&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19324386%26dopt%3DAbstract Authors: Kurtz K, Ausió J, Chiva M An interesting characteristic of decapod crustacean sperm nuclei is that they do not contain highly packaged chromatin. In the present study we re-examine the presence of DNA-interacting proteins in sperm nuclei of the brachyuran Maja brachydactyla. Although previous reports have indicated that, unlike the majority of sperm cells, DNA of decapod sperm is not organized by basic proteins, in this work we show that: (1) histones are present in sperm of M. brachydactyla; (2) histones are associated with sperm DNA; (3) histone H3 appears in lower proportions than the other core histones, while histone H2B appears in higher proportions; and (4) histone H3 in sperm nuclei is acetylated. This work complements a previous study of sperm histones of Cancer ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2301153 Tue, 24 Mar 2009 04:00:00 +0100 2301153 http://www.medworm.com/index.php?rid=2285419&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19297000%26dopt%3DAbstract Authors: Benchimol M A hydrogenosome is a hydrogen-producing organelle, evolutionary related to mitochondria and is found in Parabasalia protozoa, certain chytrid fungi and certain ciliates. It displays similarities to and differences from mitochondria. Hydrogenosomes are spherical or slightly elongated organelles, although very elongated hydrogenosomes are also found. They measure from 200nm to 1mum, but under stress conditions can reach up to 2mum. Hydrogenosomes are surrounded by two closely apposed membranes and present a granular matrix. Cardiolipin has been detected in their membranes, and frataxin, which is a conserved mitochondrial protein involved in iron metabolism, was also recently found. Hydrogenosomes have one or multiple peripheral vesicles, which incorporate calcium. Th... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2285419 Mon, 16 Mar 2009 04:00:00 +0100 2285419 http://www.medworm.com/index.php?rid=2249520&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19264336%26dopt%3DAbstract Authors: Kheradmand A, Roshangar L, Taati M, Sirotkin AV The aim of our investigation was to examine the influence of chronic administration of ghrelin on the rat ovarian state. Morphometrical and intracellular changes in the ovary of 35-d female Wistar rats after sc injection of 1nmol of ghrelin for 10 consecutive days were studied. Control animals (n=10) were injected with normal saline using similar method. The ovaries were collected on days 1 and 6 after last injection from each group and subjected to light microscopic morphometric and electron microscopic analysis. It was demonstrated that the number of corpora lutea was significantly lower and the number of ovarian follicles was higher in the treated group on days 1 and 6, than in control (P<0.01). Moreover, the mean diameter ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2249520 Tue, 03 Mar 2009 05:00:00 +0100 2249520 http://www.medworm.com/index.php?rid=2241555&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19261316%26dopt%3DAbstract Authors: Kumari U, Yashpal M, Mittal S, Mittal AK The surface ultrastructure of the gill arches and the gill rakers of an herbivorous fish, Cirrhinus mrigala was investigated by scanning electron microscopy. These structures show significant adaptive modifications associated with the food and feeding ecology of the fish. Closely lying short gill rakers and narrow inter-raker channels on the gill arches are associated to filter and retain food particles. Prominent epithelial protuberances on the gill rakers and the gill arches enable the taste buds, located at their summit, to project well above the surface of the epithelium. This could increase the efficiency of the taste buds in selective sorting of palatable food. Surface specializations of the postlingual organ are recognized adapti... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2241555 Mon, 02 Mar 2009 05:00:00 +0100 2241555 http://www.medworm.com/index.php?rid=2241554&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19261317%26dopt%3DAbstract Authors: Heng BC Human bone marrow-derived mesenchymal stem cells (MSC) have previously been reported to be susceptible to cryopreservation-induced apoptosis. A significant fraction of MSC lose their viability during freeze-thawing, which represent a major technical barrier in attaining adequate viable cell numbers for optimal efficacy in transplantation therapy. Recently, it was reported that a Rho-associated kinase (ROCK) inhibitor Y-27632 could enhance the post-thaw viability and physiological function of cryopreserved human embryonic stem cells (hESC). Hence, this study attempted to investigate whether Y-27632 can exert a similar beneficial effect on the post-thaw viability of cryopreserved MSC. A concentration range of 1-100muM Y-27632 was supplemented in both the cryopreservation... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2241554 Mon, 02 Mar 2009 05:00:00 +0100 2241554 http://www.medworm.com/index.php?rid=2234092&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19251294%26dopt%3DAbstract Authors: Chapman GB, Tarboush R, Eagles DA, Connaughton VP The distribution and ultrastructural features of peripheral nerve processes in the extra-retinal layers of the eyes of the zebrafish, Danio rerio (Hamilton), were investigated using light and transmission electron microscopy. A comparative study of the quality of preservation provided by three different fixation procedures revealed no consistently striking general differences. However, somewhat subjectively, the fixative containing Millonig's buffer did consistently provide better fixation of myelin. Overall, nerve processes, depending on the site studied, were distributed as either (1) bundles (in the choroid near the optic nerve head and in the choroid adjacent to the limbus), (2) linear arrays (in the junction between the sc... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2234092 Fri, 27 Feb 2009 05:00:00 +0100 2234092 http://www.medworm.com/index.php?rid=2234091&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19251295%26dopt%3DAbstract Authors: Tang L, Peng KM, Wang JX, Luo HQ, Cheng JY, Zhang GY, Sun YF, Liu HZ, Song H The morphology of the adrenal gland has been studied for a number of animal species all over the world, yet the detailed data about ostrich chick has not been reported. In the present study, the morphological features of the adrenal gland in African ostrich chicks were investigated by means of gross anatomy, light and electron microscope. Differences between the left and right adrenal glands were found in shape, size and location. The interrenal tissue and chromaffin cell interdigitated irregularly. The interrenal tissue was divided into a peripheral zone (PZ) and a central inner zone (CZ), and the PZ was further distinguished into an outer area (subcapsular zone, SCZ) and an inner area (IZ). The cell... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2234091 Fri, 27 Feb 2009 05:00:00 +0100 2234091 http://www.medworm.com/index.php?rid=2211513&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19233444%26dopt%3DAbstract Authors: Kumari U, Yashpal M, Mittal S, Mittal AK Glycoproteins (GPs) were visualised histochemically in the secretory cells - the mucous goblet cells (the type A and the type B), the serous goblet cells, the club cells and the epithelial cells in the gill epithelium of Rita rita. The type A mucous goblet cells, the type B mucous goblet cells and the epithelial cells elaborate GPs with oxidizable vicinal diols and GPs with sialic acid residue without O-acyl substitution. In addition, GPs with O-sulphate esters are elaborated by the type A and GPs with O-acyl sugars by the type B mucous goblet cells. GPs are absent in the serous goblet cells and are with oxidizable vicinal diols in low moieties in the club cells. The analysis of the results elucidates interesting differences in the comp... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2211513 Fri, 20 Feb 2009 05:00:00 +0100 2211513 http://www.medworm.com/index.php?rid=2191189&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19217133%26dopt%3DAbstract Authors: Jordanova M, Miteva N, Santos N, Malhão F, Rocha E When characterizing the liver ultrastructure in Ohrid trout, we noticed that cells often displayed unusual cytoplasmic crystalline inclusions. Their morphology varied much, concerning the size, shape and electron density, showing aspects never reported in fish liver. In hepatocytes, the inclusions existed in close topographical relationship with dense bodies (often within them). Such "crystals" inclusions also existed in macrophages and in biliary passages lumina. Data revealed seasonal/breeding-related changes of the dense bodies and crystalline inclusions within hepatocytes; decreasing from the earliest to the more advanced ovary maturation stages. Additionally, based on the negative correlations between the amounts of ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2191189 Wed, 11 Feb 2009 05:00:00 +0100 2191189 http://www.medworm.com/index.php?rid=2188389&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19215949%26dopt%3DAbstract Authors: Fan X, Zou R, Zhao Z, Yang P, Li Y, Song J According to previous research, integrin beta1 and ILK play an important role in the extracellular matrix (ECM)-integrin-cytoskeleton pathway for mechanotransduction. The aim of this study was to investigate strain induced integrin beta1 and ILK expression in three-dimensional (3D) and in two-dimensional (2D) cultured rat skeletal myoblasts. Sprague-Dawley (SD) rat skeletal myoblasts were isolated and seeded on the PLGA-collagen composite scaffolds. The 3D cultured and the conventionally 2D monolayer cultured myoblasts were loaded 2000mustrain tensile strain at 0.5Hz for 2h, 4h, 8h, 12h and 24h, respectively with the self-made four-point bending system. The expressions of integrin beta1 and ILK mRNA were measured by RT-PCR and the dif... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2188389 Tue, 10 Feb 2009 05:00:00 +0100 2188389 http://www.medworm.com/index.php?rid=2188388&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19215950%26dopt%3DAbstract Authors: Morgan DA, Class R, Violetta G, Soslau G Blood cells from three different sea turtle species were cultured for approximately 3 weeks in nutrient medium supplemented with recombinant human cytokines known to induce terminal maturation of human hematological stem cells. Cultured turtle erythrocytes were translucent, approximately 10x larger than human erythrocytes, contained a single fluorescent inclusion body, contained nuclear epsilon (embryonic) globin proteins, and, absent of organelles while fresh cells contained few, but well defined mitochondria. Cells with basophilic cytoplasm and in all stages of proliferation were observed in cytokine-supplemented cultures and appeared to possess active protein synthesis. Cultured thrombocytes aggregated in response to agonists for at ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2188388 Tue, 10 Feb 2009 05:00:00 +0100 2188388 http://www.medworm.com/index.php?rid=2138245&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19167737%26dopt%3DAbstract In this study, we evaluate and compare the effect of different ascorbate forms and concentrations on in vitro cartilage formation in porcine chondrocyte high-density pellet cultures. l-ascorbate, sodium l-ascorbate, and l-ascorbate-2-phosphate were administered in 100muM, 200muM, and 400muM in the culture medium over 16 days. Pellet thickness increased independently from the supplemented ascorbate form and concentration. Hydroxyproline content increased as well, but here, medium concentration of AsAP and low concentration of AsA showed a more pronounced effect. Proteoglycan and collagen formation were evaluated histologically and could be proven in all supplemented cultures. Non-supplemented cultures, however, showed no stable matrix formation at all. Effects on the gene expression pattern... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2138245 Thu, 22 Jan 2009 05:00:00 +0100 2138245 http://www.medworm.com/index.php?rid=2138244&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19167738%26dopt%3DAbstract Authors: Parillo F, Arias MP, Supplizi AV Histochemical characterization of the equine guttural pouches was performed using lectins combined with sialidase digestion and deglycosylation pre-treatments. The goblet cells contained O- and N-linked oligosaccharides with alpha-Fuc, GlcNAc moieties whereas beta-GalNAc, beta-Gal-(1-3)-GalNAc, beta-Gal-(1-4)-GlcNAc and alpha-Gal residues belonged only to O-linked glycoproteins. The acinar and ductal cells expressed alpha-Man/alpha-Glc in N-linked oligosaccharides, GlcNAc in both O- and N-glycoproteins and beta-GalNAc, beta-Gal-(1-3)-GalNAc, beta-Gal-(1-4)-GlcNAc and alpha-Gal residues included in O-linked glycoproteins. The Golgi area of the epithelial lining expressed alpha-Fuc in O-linked glycoproteins, internal GlcNAc in N-linked glycoprote... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2138244 Thu, 22 Jan 2009 05:00:00 +0100 2138244 http://www.medworm.com/index.php?rid=2101576&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19136131%26dopt%3DAbstract This study characterizes ultrastructurally the melanin granules developing in dermal melanocytes of the dorsal skin of SK, in an attempt to better understand the processes of melanogenesis in these permanently ectopic cells. The steps of melanogenesis are similar to those described for epidermal melanocytes, with melanosomes going from stage I to IV but, in SK, the maturation occurs in the cell body, as well as in the cytoplasmic processes. At stage III, the deposition of melanin is cumulative and can aggregate in rounded structures, which combine to turn into the mature granule. The final destiny of mature melanosomes is still unclear, although it was observed that dermal macrophages can accumulate melanin granules in their phagosomes. Even with the close proximity between melanocytes and... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2101576 Fri, 09 Jan 2009 05:00:00 +0100 2101576 http://www.medworm.com/index.php?rid=2101577&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19135221%26dopt%3DAbstract Authors: Machado-Santos C, Nascimento AA, Peracchi AL, Mikalauskas JS, Rocha PA, Sales A The present study was conducted to clarify the influence of feeding habits on regional distribution and relative frequency of endocrine cells secreting cholecystokinin (CCK), gastrin (GAS), serotonin (5-HT) and enteroglucagon (GLUC) in the nectarivorous Anoura geoffroyi and Glossophaga soricina and the sanguivorous Desmodus rotundus bats of the Phyllostomidae family, by specific immunohistochemical methods. The regional distribution and frequency of the different types of endocrine cells varied according to their location in the GIT. 5-HT immunoreactive cells (IR), detected throughout the GIT of three bats, were the most predominant gastrointestinal endocrine cells. GAS-IR cells in A. geoffroyi wer... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2101577 Wed, 07 Jan 2009 05:00:00 +0100 2101577 http://www.medworm.com/index.php?rid=2095185&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19131082%26dopt%3DAbstract Authors: Al-Bahry SN, Mahmoud IY, Al-Amri IS, Ba-Omar TA, Melgheit KO, Al-Kindi AY Eggshells were randomly collected from turtle nests immediately after oviposition and at the end of incubation to examine the ultrastructural features using scanning JSM-5600LV microscopy. Three layers were recognized; an outer calcareous, a middle multistrata and an inner membrane. The calcareous layer had loose nodular units varying in shape and size without interlocking attachments. In freshly laid eggs, each nodular unit had spicules arranged in folded stacks. The spicules became unfolded during incubation, to form radiating configurations. Elemental composition and mapping of the layers were analyzed using energy dispersive spectroscopy (EDS). The elements were unevenly distributed throughout the eg... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2095185 Tue, 06 Jan 2009 05:00:00 +0100 2095185 http://www.medworm.com/index.php?rid=2075182&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19111885%26dopt%3DAbstract Authors: García-Alonso J, Ruiz-Navarro A, Chaves-Pozo E, Torralva M, García-Ayala A The Spanish toothcarp Aphanius iberus is an endangered species which inhabits small rivers, creeks, salt marshes and marine salt pans in the Mediterranean coast of Spain. No differences in weights were observed among females or males taken from different environments. Analyses of the morphology of the gonads and the gametogenesis were performed in fish taken from different environments by comparing gamete development in females and in males and gonadal cell proliferation in the testis. A high degree of plasticity was observed in the gonad morphology of A. iberus. Females possess two ovaries which show non-restricted oogenesis with all germ cell stages within the same ovigerous lamellae, while ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2075182 Fri, 26 Dec 2008 05:00:00 +0100 2075182 http://www.medworm.com/index.php?rid=2027987&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19058825%26dopt%3DAbstract Authors: Alibardi L, Toni M The present study analyzes the structure and the main proteins of reptilian claws. Mature claws are formed by two to four layers of keratinocytes, a transitional layer of spindle-shaped cells and a thick corneous layer. Transitional cells elongate and merge into a compact corneous layer that is immunoreactive for beta-keratins, now indicated as sauropsid keratin-associated proteins (sKAPs). Most proteins extracted from claws in representative reptiles have a molecular weight of 13-20kDa, an acidic to basic isoelectric point, and are identified from the positive immunoreactivity to beta-keratin antibodies. The comparative analysis between lizard and avian claw beta-keratins shows the presence of an internal region of 20 amino acids with the highest identity, ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2027987 Thu, 04 Dec 2008 05:00:00 +0100 2027987 http://www.medworm.com/index.php?rid=2027986&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19058826%26dopt%3DAbstract Authors: Martinez M, Macera S, de Assis GF, Pinheiro PF, Almeida CC, Tirapelli LF, Martins OA, Mello-Júnior W, Padovani CR, Martinez FE Chronic exposure to ethanol may results in pathophysiologic changes in cellular function. The present work was designed to investigate the morphology of testis submitted to experimental ethanol ingestion. Experimental animals were divided into two groups. The control group (n=23) received a solid diet and tap water and the alcoholic group (n=23) received the same solid diet and ethanol P.A. diluted 20% in water (v/v). After 120 days of treatment, all animals were anesthetized, weighed and sacrificed. Testosterone and luteinizing hormone levels in serum were lower in the alcoholic group than in the control group. Histological and ultrastructural al... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2027986 Thu, 04 Dec 2008 05:00:00 +0100 2027986 http://www.medworm.com/index.php?rid=2018253&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19054536%26dopt%3DAbstract Authors: Tenuzzo B, Vergallo C, Dini L An increasing number of evidence indicates that static magnetic fields (SMFs) are capable of altering apoptosis, mainly through modulation of Ca(2+) influx. Here we present data that suggest apoptotic-related gene expression as an alternative pathway, through which exposure to 6milliTesla (mT) SMF can interfere with apoptosis. Exposure to 6mT SMF affects the apoptotic rate (spontaneous and drug-induced) and [Ca(2+)](i) in isolated human lymphocytes; the aged cells are more susceptible to exposure than fresh ones. The exposure to 6mT exerted a protective effect on chemical or physical-induced apoptosis, irrespective of the age of the cells. The investigation of the gene expression of bcl-2, bax, p53 and hsp70 in freshly isolated and in culture-aged... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2018253 Mon, 01 Dec 2008 05:00:00 +0100 2018253 http://www.medworm.com/index.php?rid=2006571&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19041994%26dopt%3DAbstract Authors: Díaz AO, Castro MG, García AM, Díaz de Astarloa JM, Figueroa DE Odontesthes argentinensis was collected from Mar Chiquita Coastal Lagoon, the Southernmost coastal Atlantic Lagoon of Argentina. The morphology of the gills was analyzed by scanning electron microscopy. The morphology of the superficial structures of the gill filaments and pharyngeal region of the gill arch was discussed and related to their functional aspects. The gills arches are structurally similar to those of other teleosts and bring out the osmoregulatory capacity of this species. The epithelium that covers the surface of the filaments and the pharyngeal region of the gill arch is formed by polygonal pavement cells with conspicuous microridges. These folds in the membrane are not denoted in th... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2006571 Fri, 28 Nov 2008 05:00:00 +0100 2006571 http://www.medworm.com/index.php?rid=2006570&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19041995%26dopt%3DAbstract Authors: Younes R, Ghorra C, Khalife S, Igondo-Tchen-Chantogade S, Yousfi M, Willig C, Senni K, Godeau G, Naaman N The purpose of this in situ study is to quantify the inflammatory cell subsets and the area fraction (AA%) occupied by collagen fibers in human healthy and diseased (four different stages) gingival connective tissue in order to establish a possible correlation between periodontal disease resulting in collagen breakdown and specific inflammatory cell subsets. Paraffin gingival tissue sections from eight healthy controls (group 0), 10 patients with gingivitis (group 1), 10 patients with moderate periodontitis (group 2) and 10 patients with severe periodontitis (group 3) were immunohistochemically investigated using antibodies against CD-45+, CD-3+, CD-8+, CD-20+, CD-68+, and... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=2006570 Fri, 28 Nov 2008 05:00:00 +0100 2006570 http://www.medworm.com/index.php?rid=1981408&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19019400%26dopt%3DAbstract In this study, we have investigated the regulatory effects of NO on somatotroph differentiation, proliferation and GH mRNA and protein expression using primary cell cultures of mice fetal pituitaries (embryonic days 16.5, ED 16.5). Our results show that incubation of pituitary cells in the presence of sodium nitroprusside (SNP; 1mM), a NO donor, for 4.5h resulted in a significant increase in GH mRNA and protein expression (P<0.05) and the stimulation of SNP can be inhibited by hemoglobin, a NO scavenger. But the addition of cyclic guanosine monophosphate (cGMP; 3.0mM), the second messenger of multiple NO actions cannot influence GH mRNA and protein expression. The cyclic nucleotide cellular efflux pumps existed in the pituitary cells can transport the majority of de novo-produced cGMP a... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=1981408 Mon, 17 Nov 2008 05:00:00 +0100 1981408 http://www.medworm.com/index.php?rid=1981407&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19019401%26dopt%3DAbstract Authors: Fávaro WJ, Padovani CR, Cagnon VH Diabetes causes harmful effects on prostatic function. Thus, the aims of this study were to characterize morphological and proliferative features of the prostate of diabetic mice after long-term glycemic control and testosterone and estrogen replacement. A total of 48 mice (Nod and BALBc) were used. After 20 days in a diabetic state, the mice were divided into six groups: the control group received a 5mL/kg dose of peanut oil; the diabetic group received the same treatment as the control group; the diabetic-insulin group received 4IU doses of insulin; the diabetic-testosterone group received a 5mg/kg dose of testosterone cypionate; the diabetic-estrogen group received a 25mug/kg dose of 17beta-estradiol; the diabetic-insulin-testosterone-... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=1981407 Mon, 17 Nov 2008 05:00:00 +0100 1981407 http://www.medworm.com/index.php?rid=1895723&cid=s_36114_171_f&fid=36114&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18929377%26dopt%3DAbstract Authors: Riesgo A, Maldonado M We investigated the cytology of the oogenic cycle in two oviparous demosponges, Axinella damicornis and Raspaciona aculeata, during 2 consecutive years both by light and electron microscopy. Oocytes of both species were similar in their basic morphological features but differences were noticed in time required to complete oocyte maturation and mechanisms of acquisition of nutritional reserves. The oogenic cycle of A. damicornis extended for 7-8 months in autumn-spring, while that of R. aculeata did it for 3-5 months in summer-autumn. Yolk of A. damicornis was predominantly formed by autosynthesis. Oocytes endocytosed bacteria individually and stored them in groups in large vesicles. Bacteria were digested and lipidic material was added to the vesicles to ... Tissue and Cell journals http://www.medworm.com/rss/comments.php?id=1895723 Wed, 15 Oct 2008 04:00:00 +0100 1895723