MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Transgenic Research' source. Sun, 21 Mar 2010 16:44:45 +0100 http://www.medworm.com/index.php?rid=3284231&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20157802%26dopt%3DAbstract Program and Abstracts of the 9th Transgenic Technology Meeting (TT2010) : 22-24 March 2010, Conference Centre, Max Delbrück Center for Molecular Medicine [MDC], Robert-Rössle-Str. 10, 13125 Berlin, Germany. Transgenic Res. 2010 Apr;19(2):307-55 Authors: PMID: 20157802 [PubMed - in process] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3284231 Thu, 18 Feb 2010 22:48:05 +0100 3284231 http://www.medworm.com/index.php?rid=3276419&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20155397%26dopt%3DAbstract Authors: Santos MN, Nogueira PM, Dias FB, Valle D, Moreira LA Vector-born diseases cause millions of deaths every year globally. Alternatives for the control of diseases such as malaria and dengue fever are urgently needed and the use of transgenic mosquitoes that block parasite/virus is a sound strategy to be used within control programs. However, prior to use transgenic mosquitoes as control tools, it is important to study their fitness since different biological aspects might influence their ability to disseminate and compete with wild populations. We previously reported the construction of four transgenic Aedes fluviatilis mosquito lines expressing a Plasmodium- blocking molecule (mutated bee venom phospholipase A(2)-mPLA(2)). Presently we studied two aspects of their fitness: body... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3276419 Sat, 13 Feb 2010 00:00:00 +0100 3276419 http://www.medworm.com/index.php?rid=3271373&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151323%26dopt%3DAbstract We examined the efficacy of this approach in cotton by constitutive expression of the Arabidopsis (Arabidopsis thaliana) NPR1 gene. The results show that NPR1-expressing lines exhibited significant resistance to Verticillium dahliae isolate TS2, Fusarium oxysporum f. sp. vasinfectum, Rhizoctonia solani, and Alternaria alternata. Interestingly, the transformants also showed significant resistance to reniform nematodes. Analysis of defense-related, biochemical and molecular responses suggest that when challenged with pathogens or certain systemic acquired resistance-inducing chemicals, the transgenic lines respond to a greater degree compared to the wild-type plants. Importantly, the basal activities of the defense-related genes and enzymes in uninduced transformants were no different than t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3271373 Fri, 12 Feb 2010 00:00:00 +0100 3271373 http://www.medworm.com/index.php?rid=3259984&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20143261%26dopt%3DAbstract Authors: De Repentigny Y, Kothary R Generation of mouse chimeras is useful for the elucidation of gene function. In the present report, we describe a new technique for the production of chimeras by injection of R1 embryonic stem (ES) cells into the perivitelline space of one-cell stage mouse embryos. One-cell embryos are injected with 2-6 ES cells into the perivitelline space under the zona pellucida without laser-assistance. Our embryo culture experiments reveal that ES cells injected at the one-cell stage embryo start to be incorporated into the blastomeres beginning at the 8-cell stage and form a chimeric blastocyst after 4 days. We have used this approach to successfully produce a high rate of mouse chimeras in two different mouse genetic backgrounds permitting the establishment of... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3259984 Tue, 09 Feb 2010 00:00:00 +0100 3259984 http://www.medworm.com/index.php?rid=3252237&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140643%26dopt%3DAbstract Authors: Duan G, Saint RB, Helliwell CA, Behm CA, Waterhouse PM, Gordon KH Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT-PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development. PMID: 20140643 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3252237 Sat, 06 Feb 2010 00:00:00 +0100 3252237 http://www.medworm.com/index.php?rid=3248734&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20135222%26dopt%3DAbstract Authors: Jackson KA, Berg JM, Murray JD, Maga EA While there are many reports in the literature describing the attributes of specific applications of transgenic animals for agriculture, there are relatively few studies focusing on the fitness of the transgenic animals themselves. This work was designed to gather information on genetically modified food animals to determine if the presence of a transgene can impact general animal production traits. More specifically, we used a line of transgenic dairy goats expressing human lysozyme in their mammary gland to evaluate the reproductive fitness and growth and development of these animals compared to their non-transgenic counterparts and the impact of consuming a transgenic food product, lysozyme-containing milk. In males, none of the param... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3248734 Fri, 05 Feb 2010 00:00:00 +0100 3248734 http://www.medworm.com/index.php?rid=3248733&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20135223%26dopt%3DAbstract In this study, we report results from a field trial on three rolB transgenic dwarfing apple rootstocks of M26 and M9 together with non-transgenic controls grafted with five non-transgenic scion cultivars. We intended to investigate the effects of transgenic rootstock on non-transgenic scion cultivars under natural conditions as well as to evaluate the potential value of using the rolB gene to modify difficult-to-root rootstocks of fruit trees. The results showed that all rolB transgenic rootstocks significantly reduced vegetative growth including tree height regardless of scion cultivar, compared with the non-transgenic rootstocks. Flowering and fruiting were also decreased for cultivars grown on the transgenic rootstocks in most cases, but the fruit quality was not clearly affected by the... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3248733 Fri, 05 Feb 2010 00:00:00 +0100 3248733 http://www.medworm.com/index.php?rid=3244269&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127510%26dopt%3DAbstract Authors: Pavli OI, Panopoulos NJ, Goldbach R, Skaracis GN Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in morphology to wild type roots but were characterized by a profound abundancy, rapid growth rate and, in some cases, plagiotropic development. Upon challenge inoculation, seedlings showed a considerable delay in symptom development compared to untransformed or vector-transformed seedlings, expressing dsRNA from an unrelated source. The transgenic root system of almost all seedlings contained no or very low virus titer w... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3244269 Wed, 03 Feb 2010 00:00:00 +0100 3244269 http://www.medworm.com/index.php?rid=3223749&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107893%26dopt%3DAbstract Authors: Le Saux A, Houdebine LM, Jolivet G This paper reports our attempts to characterize transgene integration sites in transgenic mouse lines generated by the microinjection of large (from 30 to 145 kb) pig DNA fragments encompassing a mammary specific gene, the whey acidic protein gene (WAP). Among the various methods used, the thermal asymmetric interlaced (TAIL-) PCR method allowed us (1) to analyze transgene/genomic borders and internal concatamer junctions for eleven transgenic lines, (2) to obtain sequence information for seven borders, (3) to place three transgenes in the mouse genome, and (4) to obtain sequence data for seven transgene junctions in concatamers. Finally, we characterized various rearrangements in the borders and the inner parts of the transgene. The possibil... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3223749 Wed, 27 Jan 2010 00:00:00 +0100 3223749 http://www.medworm.com/index.php?rid=3223748&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107894%26dopt%3DAbstract In this study, the rapeseed cultivar, Yangyou 4, was genetically engineered via Agrobacterium-mediated transformation to express the hrf2 gene. Polymerase chain reaction (PCR) and southern blot analyses of T(1) generation of transgenic rapeseed revealed stable integration and expression of the inserted gene hrf2. In addition, the resistance to Sclerotinia sclerotiorum was greatly enhanced. A comparison between agronomic characters of transgenic and control lines displayed significant differences in terms of plant height, stem width, number of pods per plant, number of seeds per pod, 1,000-seed weight, and seed yield per plant. Among lines with resistance to S. sclerotiorum, T(1)1 had improved agronomic traits compared with controls with a 22.7% seed yield increase. These results suggest th... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3223748 Wed, 27 Jan 2010 00:00:00 +0100 3223748 http://www.medworm.com/index.php?rid=3223747&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20107895%26dopt%3DAbstract Authors: Samano AK, Ohshima-Hosoyama S, Whitney TG, Prajapati SI, Kilcoyne A, Taniguchi E, Morgan WW, Nelon LD, Lin AL, Togao O, Jung I, Rubin BP, Nowak BM, Duong TQ, Keller C Medulloblastoma is an aggressive childhood cerebellar tumor. We recently reported a mouse model with conditional deletion of Patched1 gene that recapitulates many characteristics of the human medulloblastoma. Qualitative symptoms observed in the mouse model include irregular stride length, impaired cranial nerve function and decreased motor coordination and performance. In our current study, several quantitative behavioral assays including a mouse rotarod, a forced air challenge, a screen inversion test, a horizontal wire test, and stride length analysis were evaluated to determine the most sensitive and cost-eff... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3223747 Wed, 27 Jan 2010 00:00:00 +0100 3223747 http://www.medworm.com/index.php?rid=3209377&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099029%26dopt%3DAbstract This study was undertaken to characterize the effects of constitutive expression of the hedgehog transcriptional activator, Gli2, in porcine skin. The keratinocyte-specific human transgene, K5-hGli2DeltaN, was used to produce transgenic porcine lines via somatic cell nuclear transfer techniques. In mice, K5-hGli2DeltaN induces epithelial downgrowths resembling basal cell carcinomas. Our porcine model also developed these basal cell carcinoma-like lesions, however gross tumor development was not appreciated. In contrast to the murine model, diffuse epidermal changes as well as susceptibility to cutaneous infections were seen in the swine model. Histologic analysis of transgenic piglets revealed generalized epidermal changes including: epidermal hyperplasia (acanthosis), elongated rete ridge... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3209377 Sat, 23 Jan 2010 00:00:00 +0100 3209377 http://www.medworm.com/index.php?rid=3200779&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20091347%26dopt%3DAbstract In this study, using lentiviral eGFP transgenic mice as the experimental animals, lentiviral eGFP expression levels and its integrant numbers in genome were quantitatively analyzed by fluorescent quantitative polymerase-chain reaction (FQ-PCR), using the house-keeping gene ribosomal protein S18 (Rps18) and the single copy gene fatty acid binding protein of the intestine (Fabpi) as the internal controls respectively. The methylation densities of the integrants were quantitatively analyzed by bisulfite sequencing. We found that the lentiviral integrants with high expression exhibited a relative constant expression level per integrant over at least seven generations. Besides, the individuals containing these integrants exhibited eGFP expression levels which were positively and almost linearly... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3200779 Fri, 22 Jan 2010 00:00:00 +0100 3200779 http://www.medworm.com/index.php?rid=3200778&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20094912%26dopt%3DAbstract Authors: Remy S, Tesson L, Usal C, Menoret S, Bonnamain V, Nerriere-Daguin V, Rossignol J, Boyer C, Nguyen TH, Naveilhan P, Lescaudron L, Anegon I Adoptive cell transfer studies in regenerative research and identification of genetically modified cells after gene therapy in vivo require unequivocally identifying and tracking the donor cells in the host tissues, ideally over several days or for up to several months. The use of reporter genes allows identifying the transferred cells but unfortunately most are immunogenic to wild-type hosts and thus trigger rejection in few days. The availability of transgenic animals from the same strain that would express either high levels of the transgene to identify the cells or low levels but that would be tolerant to the transgene would allow perfor... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3200778 Fri, 22 Jan 2010 00:00:00 +0100 3200778 http://www.medworm.com/index.php?rid=3194257&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20087655%26dopt%3DAbstract Authors: Cueno ME, Hibi Y, Imai K, Laurena AC, Okamoto T Previous attempts to express the human immunodeficiency virus 1 (HIV-1) Tat (trans-activator of transcription) protein in plants resulted in a number of physiological abnormalities, such as stunted growth and absence of seed formation, that could not be explained. In the study reported here, we expressed Tat in tomato and observed phenotypic abnormalities, including stunted growth, absence of root formation, chlorosis, and plant death, as a result of reduced cytokinin levels. These reduced levels were ascribed to a differentially expressed CKO35 in Tat-bombarded tomato. Of the two CKO isoforms that are naturally expressed in tomato, CKO43 and CKO37, only the expression of CKO37 was affected by Tat. Our analysis of the Tat confirm... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3194257 Wed, 20 Jan 2010 00:00:00 +0100 3194257 http://www.medworm.com/index.php?rid=3194256&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20087656%26dopt%3DAbstract Authors: Zhang H, Liu W, Wan L, Li F, Dai L, Li D, Zhang Z, Huang R Ethylene response factor (ERF) proteins play important roles in regulating plant stress response and development. Our previous studies have shown that JERF3 activates the expression of oxidative stress responsive genes in transgenic tobacco and enhances tolerance to salt, drought, and freezing, indicating that JERF3 is a very important transcriptional regulator in dicot plants. In the study reported here, we further addressed the regulatory function of JERF3 in a monocot, rice, by generating transgenic rice plants overexpressing JERF3 and comparing these with non-transgenic rice plants for physiological and molecular alterations and tolerance to drought and osmotic stresses. Growth and development under normal growth c... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3194256 Wed, 20 Jan 2010 00:00:00 +0100 3194256 http://www.medworm.com/index.php?rid=3194258&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20084547%26dopt%3DAbstract Authors: Motoyama T, Okumoto Y, Tanisaka T, Utsumi S, Maruyama N A transgenic rice that produces both the alpha' and beta subunits of beta-conglycinin has been developed through the crossing of two types of transgenic rice. Although the accumulation level of the alpha' subunit in the alpha'beta-transgenic rice was slightly lower than that in the transgenic rice producing only the alpha' subunit, the accumulation level of the beta subunit in the alpha'beta-transgenic rice was about 60% higher than that in the transgenic rice producing only the beta subunit. Results from sequential extraction and gel-filtration experiments indicated that part of the beta subunit formed heterotrimers with the alpha' subunit in a similar manner as in soybean seeds and that the heterotrimers interacted with... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3194258 Tue, 19 Jan 2010 00:00:00 +0100 3194258 http://www.medworm.com/index.php?rid=3186057&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20082138%26dopt%3DAbstract Authors: Peremarti A, Bassie L, Zhu C, Christou P, Capell T Arginine decarboxylase (ADC) is a key enzyme in plants that converts arginine into putrescine, an important mediator of abiotic stress tolerance. Adc genes have been isolated from a number of dicotyledonous plants but the oat and rice Adc genes are the only representatives of monocotyledonous species described thus far. Rice has a small family of Adc genes, and OsAdc1 expression has been shown to fluctuate under drought and chilling stress. We identified and characterized a second rice Adc gene (OsAdc2) which encodes a 629-amino-acid protein with a predicted molecular mass of 67 kDa. An unusual feature of the OsAdc2 gene is the presence of an intron and a short upstream open reading frame in the 5'-UTR. Sequence comparisons sh... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3186057 Sat, 16 Jan 2010 00:00:00 +0100 3186057 http://www.medworm.com/index.php?rid=3176664&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20072814%26dopt%3DAbstract Authors: Young R, Wolf CR, Brown K, Hayes JD, Whitelaw CB Transgenic reporter mice can contribute in the development of less toxic and more selective drugs to treat disease. In this brief communication we describe the generation and initial validation of transgenic mice that provide a visual spatial readout of oxidative stress. These mice carry a LacZ reporter transgene driven by the human haem oxygenase 1 promoter. The induction of LacZ staining by a range of compounds indicated differences in the haem oxygenase 1 spatial response within a tissue. Thus this transgene allows for the spatial monitoring of differences in toxic insult and indicates that this type of transgenic system could have use in toxicity screens. PMID: 20072814 [PubMed - as supplied by publisher] (Source: Transg... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3176664 Thu, 14 Jan 2010 00:00:00 +0100 3176664 http://www.medworm.com/index.php?rid=3176663&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20072815%26dopt%3DAbstract In this study, we created a plant-optimized tat and mutant (Cys30Ala/Lys41Ala) tat (mtat) gene and ligated each into a pBI121 expression vector with a stop codon and a gusA gene positioned immediately downstream. The vector construct was bombarded into tomato leaf calli and allowed to develop. We thus generated recombinant tomato plants preferentially expressing a Tat-GUS fusion protein over a Tat-only protein. In addition, plants bombarded with either tat or mtat genes showed no phenotypic difference and produced 2-4 mug Tat-GUS fusion protein per milligram soluble plant protein. Furthermore, tomato extracts intradermally inoculated into mice were found to induce a humoral and, most importantly, cellular immunity. PMID: 20072815 [PubMed - as supplied by publisher] (Source: Transgenic ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3176663 Thu, 14 Jan 2010 00:00:00 +0100 3176663 http://www.medworm.com/index.php?rid=3168254&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069454%26dopt%3DAbstract Authors: Hiripi L, Negre D, Cosset FL, Kvell K, Czömpöly T, Baranyi M, Gócza E, Hoffmann O, Bender B, Bősze Z Transgenic rabbit is the preferred disease model of atherosclerosis, lipoprotein metabolism and cardiovascular diseases since upon introducing genetic mutations of human genes, rabbit models reflect human physiological and pathological states more accurately than mouse models. Beyond that, transgenic rabbits are also used as bioreactors to produce pharmaceutical proteins in their milk. Since in the laboratory rabbit the conventional transgenesis has worked with the same low efficiency in the last twenty five years and truly pluripotent embryonic stem cells are not available to perform targeted mutagenesis, our aim was to adapt lentiviral transgenesis to thi... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3168254 Wed, 13 Jan 2010 00:00:00 +0100 3168254 http://www.medworm.com/index.php?rid=3136963&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20044792%26dopt%3DAbstract Authors: Zhu B, Ma BL, Blackshaw RE Real time PCR assays were developed to detect and quantify the transgene DNA of a commercially released Bacillus thuringiensis (Bt) corn (Zea mays L.) hybrid (DKC42-23), which was derived from the event MON863 and also carried a neomycin phosphotransferase gene (the nptII gene). We applied the real time PCR assays to investigate the persistence of the transgene DNA in a field trial grown with DKC42-23 over 3 years, in combination with bacterial natural transformation. The results showed that under continuous cultivation of DKC42-23, its transgene DNA was detectable in the field plots all year around. Meanwhile, when soil DNA extracts from DKC42-23 plots were used as donor in bacterial natural transformation, successful recovery of kanamycin resistant... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3136963 Fri, 01 Jan 2010 00:00:00 +0100 3136963 http://www.medworm.com/index.php?rid=3102488&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20012775%26dopt%3DAbstract Authors: Rauschen S, Schaarschmidt F, Gathmann A Beetles (Coleoptera) are a diverse and ecologically important group of insects in agricultural systems. The Environmental Risk Assessment (ERA) of genetically modified Bt-crop varieties with insect resistances thus needs to consider and assess the potential negative impacts on non-target organisms belonging to this group. We analysed data gathered during 6 years of field-release experiments on the impact of two genetically modified Bt-maize varieties (Ostrinia-resistant MON810 and Diabrotica-resistant MON88017) on the occurrence and field densities of Coleoptera, especially the two families Coccinellidae and Chrysomelidae. Based on a statistical analysis aimed at establishing whether Bt-maize varieties are equivalent to their near-isogen... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3102488 Sat, 12 Dec 2009 00:00:00 +0100 3102488 http://www.medworm.com/index.php?rid=3102490&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20012190%26dopt%3DAbstract This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. However, no evident morphological abnormalities were observed. Thus, overexpression of Ptmaa alone is not sufficient to cause disorganized growths or even cancer in zebrafish skin. Molecular and histological evidences showed that Tg(k18:Ptmaa-RFP) embryos have more proliferating cells in the pelvic fins [WT: 3.92 +/- 7.15; Tg(k18:Ptmaa-RFP): 38.00 +/- 10.87] and thicker skin [WT: 10.98 +/- 1.41 mum; Tg(k18:Ptmaa-RFP): 14.02 +/- 1.32 mum], indicating that overexpression of Ptmaa... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3102490 Fri, 11 Dec 2009 00:00:00 +0100 3102490 http://www.medworm.com/index.php?rid=3102489&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20012191%26dopt%3DAbstract Authors: Zhou Z, Wang MJ, Zhao ST, Hu JJ, Lu MZ In plant species, the level of polyunsaturated fatty acids (PUFAs) is essential for cold acclimation. To test whether changes in PUFA levels can lead to the alteration of freezing tolerance in poplar trees, we up- and down-regulated a Populus tomentosa Delta-12 fatty acid desaturase gene (PtFAD2) in the hybrid poplar (P. alba x P. glandulosa) clone 84 K. Real-time PCR results demonstrated that compared to untransformed control lines, the transcriptional level of PtFAD2 increased by up to 90% in over-expressing poplar lines (line OE-1) and decreased in down-regulated RNAi lines by up to 64% (line DR-1). As a result, the content of linoleic (C18:2) and linolenic (C18:3) unsaturated fatty acids (FAs) in total FAs increased by 7.5 and 3.9%, r... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3102489 Thu, 10 Dec 2009 00:00:00 +0100 3102489 http://www.medworm.com/index.php?rid=3056851&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19953346%26dopt%3DAbstract In this report, we translationally fused the 2L21 peptide to the 42 amino acid tetramerisation domain (TD) from the human transcription factor p53. The chimaeric 2L21-TD protein was expressed in tobacco chloroplasts. Leaves accumulated high levels of the recombinant protein (up to 0.4 mg/g fresh weight of leaf material, equivalent to ~6% of total soluble protein; 2% considering only the 2L21 peptide). The 2L21-TD protein was able to form tetramers in the stroma of the chloroplast. Mice immunised intraperitoneally with partially purified leaf extracts containing the 2L21-TD protein developed specific antibodies with titres similar to those elicited by a previously reported fusion between 2L21 and the B subunit of the cholera toxin. Mouse sera were able to detect both the 2L21 synthetic pept... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3056851 Wed, 02 Dec 2009 00:00:00 +0100 3056851 http://www.medworm.com/index.php?rid=3036248&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943109%26dopt%3DAbstract Authors: Kung YJ, Yu TA, Huang CH, Wang HC, Wang SL, Yeh SD Papaya production is seriously limited by Papaya ringspot virus (PRSV) worldwide and Papaya leaf-distortion mosaic virus (PLDMV) in Eastern Asia. An efficient transformation method for developing papaya lines with transgenic resistance to these viruses and commercially desirable traits, such as hermaphroditism, is crucial to shorten the breeding program for this fruit crop. In this investigation, an untranslatable chimeric construct pYP08 containing truncated PRSV coat protein (CP) and PLDMV CP genes coupled with the 3' untranslational region of PLDMV, was generated. Root segments from different portions of adventitious roots of in vitro multiple shoots of hermaphroditic plants of papaya cultivars 'Tainung No. 2', 'Sunrise', a... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3036248 Fri, 27 Nov 2009 00:00:00 +0100 3036248 http://www.medworm.com/index.php?rid=3030853&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937380%26dopt%3DAbstract Authors: Paul E, Kielbasinski M, Sedivy JM, Murga-Zamalloa C, Khanna H, Klysik JE Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights into the spatial and temporal expression of Supv3L1 in the mouse, we generated knock-in and transgenic strains in which an EGFP reporter was placed under control of the Supv3L1 native promoter. During development, expression of Supv3L1 begins at the blastocyst stage, becomes widespread and strong in all fetal tissues and cell types, and continues during postnatal growth. In mature animals repo... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3030853 Wed, 25 Nov 2009 00:00:00 +0100 3030853 http://www.medworm.com/index.php?rid=3030854&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937273%26dopt%3DAbstract The objective of the current study was 2-fold: (1) to compare the effectiveness of a single 1 ms pulse of different voltages (V; 100, 150, 200, 250, 300, 350) and multiple 1 ms pulses (1, 2, 3, 4 or 5) at 300 V for delivery and expression of super-coiled GFP vector in surviving cells of three fetal fibroblast cell lines, and (2) to determine the ability of these electroporation parameters to produce stably transfected fibroblast colonies following G418 selection. Cell line (P < 0.001) and voltage (P < 0.001) affected DNA delivery into the cell as assessed by GFP expression while survival at 24 h was affected by voltage (P < 0.001) and not by cell line (P = 0.797). Using a single pulse while increasing voltage resulted in the percentage of GFP expressing cells increasing from 3.2 +... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3030854 Tue, 24 Nov 2009 00:00:00 +0100 3030854 http://www.medworm.com/index.php?rid=3015506&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19924556%26dopt%3DAbstract Authors: Raybould A, Tuttle A, Shore S, Stone T The environmental risks from cultivating crops producing output trait enzymes can be rigorously assessed by testing conservative risk hypotheses of no harm to endpoints such as the abundance of wildlife, crop yield and the rate of degradation of crop residues in soil. These hypotheses can be tested with data from many sources, including evaluations of the agronomic performance and nutritional quality of the crop made during product development, and information from the scientific literature on the mode-of-action, taxonomic distribution and environmental fate of the enzyme. Few, if any, specific ecotoxicology or environmental fate studies are needed. The effective use of existing data means that regulatory decision-making, to which an envi... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=3015506 Thu, 19 Nov 2009 00:00:00 +0100 3015506 http://www.medworm.com/index.php?rid=2972102&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19894134%26dopt%3DAbstract Authors: Andersson KB, Winer LH, Mørk HK, Molkentin JD, Jaisser F Tissue-specific and time-dependent control of in vivo gene disruption may be achieved using conditional knockout strategies in transgenic mice. Fusion of mutant estrogen receptor ligand-binding domains to Cre recombinase (Cre-ER(T), MerCreMer) combined with cardiac-directed gene expression has been used to generate several cardiac-specific tamoxifen-inducible Cre-expressing mouse lines. Such mice have successfully been used to generate Cre-loxP-mediated gene disruption in an inducible manner in the myocardium in vivo. However, information is sparse regarding the tamoxifen dosage, the time course of gene disruption and whether different administration routes differ in efficiency in obtaining gene disruption in the my... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2972102 Fri, 06 Nov 2009 00:00:00 +0100 2972102 http://www.medworm.com/index.php?rid=2967879&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888667%26dopt%3DAbstract Authors: Murray JD, Maga EA PMID: 19888667 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2967879 Wed, 04 Nov 2009 00:00:00 +0100 2967879 http://www.medworm.com/index.php?rid=2967878&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19888668%26dopt%3DAbstract Authors: Paul V, Guertler P, Wiedemann S, Meyer HH To investigate the relative degradation and fragmentation pattern of the recombinant Cry1Ab protein from genetically modified (GM) maize MON810 throughout the gastrointestinal tract (GIT) of dairy cows, a 25 months GM maize feeding study was conducted on 36 lactating Bavarian Fleckvieh cows allocated into two groups (18 cows per group) fed diets containing either GM maize MON810 or nearly isogenic non-GM maize as the respective diet components. All cows were fed a partial total mixed ration (pTMR). During the feeding trial, 8 feed (4 transgenic (T) and 4 non-transgenic (NT) pTMR) and 42 feces (26 T and 18 NT) samples from the subset of cows fed T and NT diets, and at the end of the feeding trial, digesta contents of rumen, abomasum, sm... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2967878 Wed, 04 Nov 2009 00:00:00 +0100 2967878 http://www.medworm.com/index.php?rid=2959538&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19882223%26dopt%3DAbstract Authors: Ménoret S, Tesson L, Remy S, Usal C, Iscache AL, Anegon I PMID: 19882223 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2959538 Sun, 01 Nov 2009 00:00:00 +0100 2959538 http://www.medworm.com/index.php?rid=2940459&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19862637%26dopt%3DAbstract Authors: Teh YH, Kavanagh TA Nanobodies (or VHHs) are single-domain antigen-binding fragments derived from Camelid heavy chain-only antibodies. Their small size, monomeric behaviour, high stability and solubility, and ability to bind epitopes not accessible to conventional antibodies make them especially suitable for many therapeutic and biotechnological applications. Here we describe high-level expression, in Nicotiana benthamiana, of three versions of an anti-hen egg white lysozyme (HEWL) nanobody which include the original VHH from an immunized library (cAbLys3), a codon-optimized derivative, and a codon-optimized hybrid nanobody comprising the CDRs of cAbLys3 grafted onto an alternative 'universal' nanobody framework. His6- and StrepII-tagged derivatives of each nanobody were targe... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2940459 Wed, 28 Oct 2009 00:00:00 +0100 2940459 http://www.medworm.com/index.php?rid=2940458&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19862638%26dopt%3DAbstract In this study, we investigated the transmission of LV-PGK-EGFP integrants through the female and male germ line in cattle. A transgenic founder heifer (#562, Kiki) was subjected to superovulation treatment and inseminated with semen from a non-transgenic bull. Embryos were recovered and transferred to synchronized recipient heifers, resulting in the birth of a healthy male transgenic calf expressing EGFP as detected by in vivo imaging. Semen from a transgenic founder bull (#561, Jojo) was used for in vitro fertilization (IVF) of in vitro matured (IVM) oocytes from non-transgenic cows. The rates of cleavage and development to blastocyst in vitro corresponded to 52.0 +/- 4.1 and 24.5 +/- 4.4%, respectively. Expression of EGFP was observed at blastocyst stage (day 7 after IVF) and was seen in... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2940458 Wed, 28 Oct 2009 00:00:00 +0100 2940458 http://www.medworm.com/index.php?rid=2923769&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19851881%26dopt%3DAbstract Authors: Kim S, Lee DS, Choi IS, Ahn SJ, Kim YH, Bae HJ Over the past decade various approaches have been used to increase the expression level of recombinant proteins in plants. One successful approach has been to target proteins to specific subcellular sites/compartments of plant cells, such as the chloroplast. In the study reported here, hyperthermostable endoglucanase Cel5A was targeted into the chloroplasts of tobacco plants via the N-terminal transit peptide of nuclear-encoded plastid proteins. The expression levels of Cel5A transgenic lines were then determined using three distinct transit peptides, namely, the light-harvesting chlorophyll a/b-binding protein (CAB), Rubisco small subunit (RS), and Rubisco activase (RA). RS:Cel5A transgenic lines produced highly stable active enz... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2923769 Thu, 22 Oct 2009 23:00:00 +0100 2923769 http://www.medworm.com/index.php?rid=2923771&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19847665%26dopt%3DAbstract Authors: Gao YL, Feng HQ, Wu KM Transgenic cotton expressing the Bacillus thuringiensis (Bt) Cry1Ac toxin has been commercially cultivated in China since 1997, and by 2000 Bt cotton had almost completely replaced non-transgenic cotton cultivars. To evaluate the impact of Bt cotton planting on the seasonal population patterns of cotton bollworm, Helicoverpa armigera, the dynamics of H. armigera moths were monitored with light traps from four locations (Xiajin, Linqing and Dingtao of Shandong Province; Guantao of Hebei Province) in high Bt density region and five locations (Anci and Xinji of Hebei Province; Dancheng and Fengqiu of Henan Province; Gaomi of Shandong Province) in low Bt density region from 1996 to 2008. A negative correlation was found between moth densities of H. armigera ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2923771 Wed, 21 Oct 2009 23:00:00 +0100 2923771 http://www.medworm.com/index.php?rid=2923770&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19847666%26dopt%3DAbstract The objective of this study was to further examine the effect of consuming hLZ-containing milk on young pigs by profiling serum metabolites. Pigs were placed into two groups and fed a diet of solid food and either control (non-transgenic) goats' milk or milk from hLZ-transgenic goats for 6 weeks. Serum samples were collected at the end of the feeding period and global metabolite profiling was performed. For a total of 225 metabolites (160 known, 65 unknown) semi-quantitative data was obtained. Levels of 18 known and 4 unknown metabolites differed significantly between the two groups with the direction of change in 13 of the 18 known metabolites being almost entirely congruent with improved health status, particularly in terms of the gastrointestinal tract health and immune response, with t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2923770 Wed, 21 Oct 2009 23:00:00 +0100 2923770 http://www.medworm.com/index.php?rid=2923772&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844803%26dopt%3DAbstract This study was designed to determine whether a genetic modification, knock out (KO) or transgenics, not anticipated to affect reproductive performance could affect the resulting reproductive profile of the newly developed transgenic mouse. More specifically, this study is designed to study the impact of the genetic modification on the ability of gametes to be fertilized in vitro. We analyzed the reproductive performance of mice with different background strains: FVB/N, C57BL/6 (129Sv/J x C57Bl/6)F1 and outbred CD1((R)) and compared them to mice of the same strain carrying a transgene or KO which was not anticipated to affect fertility. In vitro Fertilization was used to analyze the fertility of the mice. Oocytes from superovulated females were inseminated with sperm of same background. Fer... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2923772 Tue, 20 Oct 2009 23:00:00 +0100 2923772 http://www.medworm.com/index.php?rid=2923773&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19842055%26dopt%3DAbstract Authors: Fielder TJ, Barrios L, Montoliu L The generation of transgenic mice by microinjection of DNA into the pronuclei of fertilized oocytes was described in the early 1980s. A number of parameters affecting the efficiency of the technique were soon identified, including the type of DNA construct, the concentration of DNA being injected, and, most importantly, the strain of mice used for oocyte donors. Since then, hundreds of laboratories and transgenic core facilities across the world have successfully used this technique, essentially as originally described, to create thousands of new transgenic mouse lines. However, the overall procedure continues to be relatively inefficient, in terms of the number of fertilized oocytes required to produce a transgenic mouse, and variations in yi... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2923773 Mon, 19 Oct 2009 23:00:00 +0100 2923773 http://www.medworm.com/index.php?rid=2894909&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19826906%26dopt%3DAbstract Authors: Henquet M, Heinhuis B, Borst JW, Eigenhuijsen J, Schreuder M, Bosch D, van der Krol A In plants and animals, the first step in complex type N-glycan formation on glycoproteins is catalyzed by N-acetylglucosaminyltransferase I (GnTI). We show that the cgl1-1 mutant of Arabidopsis, which lacks GnTI activity, is fully complemented by YFP-labeled plant AtGnTI, but only partially complemented by YFP-labeled human HuGnTI and that this is due to post-transcriptional events. In contrast to AtGnTI-YFP, only low levels of HuGnTI-YFP protein was detected in transgenic plants. In protoplast co-transfection experiments all GnTI-YFP fusion proteins co-localized with a Golgi marker protein, but only limited co-localization of AtGnTI and HuGnTI in the same plant protoplast. The partial altern... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2894909 Tue, 13 Oct 2009 23:00:00 +0100 2894909 http://www.medworm.com/index.php?rid=2881395&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19816782%26dopt%3DAbstract Authors: Wever W, McCallum EJ, Chakravorty D, Cazzonelli CI, Botella JR The structure and function of untranslated mRNA leader sequences and their role in controlling gene expression remains poorly understood. Previous research has suggested that the 5' untranslated region (5'UTR) of the Vigna radiata aminocyclopropane-1-carboxylate synthase synthase (VR-ACS1) gene may function as a translational enhancer in plants. To test such hypothesis we compared the translation enhancing properties of three different 5'UTRs; those from the VR-ACS1, the chlorophyll a/b binding gene from petunia (Cab22L; a known translational enhancer) and the Vigna radiata pectinacetylesterase gene (PAE; used as control). Identical constructs in which the coding region of the beta-glucuronidase (GUS) gene was fuse... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2881395 Thu, 08 Oct 2009 23:00:00 +0100 2881395 http://www.medworm.com/index.php?rid=2876905&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19809883%26dopt%3DAbstract Authors: PMID: 19809883 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2876905 Tue, 06 Oct 2009 23:00:00 +0100 2876905 http://www.medworm.com/index.php?rid=2876904&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19809884%26dopt%3DAbstract Authors: Houdebine LM PMID: 19809884 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2876904 Tue, 06 Oct 2009 23:00:00 +0100 2876904 http://www.medworm.com/index.php?rid=2852927&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19795218%26dopt%3DAbstract In this study, we report successful production of transgenic chickens that express the human erythropoietin (hEPO) gene under the control of a tetracycline-inducible promoter. A recombinant Moloney murine leukemia virus (MoMLV)-based retrovirus vector encapsidated with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of unincubated chicken embryos (stage X). Out of 198 injected eggs, 15 chicks hatched after 21 days of incubation and 14 hatched chicks expressed the vector-encoded hEPO gene when fed doxycycline, a tetracycline derivative, without any significant physiological dysfunctions. The expression of hEPO reverted to the pre-induction state by removing doxycycline from the diet. The biological activity of the hEPO produced in the transgenic chicken... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2852927 Wed, 30 Sep 2009 23:00:00 +0100 2852927 http://www.medworm.com/index.php?rid=2852929&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789990%26dopt%3DAbstract Authors: Tatematsu KI, Kobayashi I, Uchino K, Sezutsu H, Iizuka T, Yonemura N, Tamura T To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream region of Ser1 was strong, yielding high expression of an enhanced green fluorescent protein (EGFP) transgene in the middle and posterior regions of the middle silk gland (MSG) after day 2 of the fifth instar. The Ser3 upstream region exhibited moderate promoter activity in the anterior MSG, but the Ser2 upstream region did not exhibit any promoter activity. Since the strongest promoter act... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2852929 Tue, 29 Sep 2009 23:00:00 +0100 2852929 http://www.medworm.com/index.php?rid=2852928&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789991%26dopt%3DAbstract Authors: Wang B, Zhou X, Xu F, Gao J Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a common and economically important crop in Asia. Specific targets of plant breeding programs for cabbage have been improvement in yield, resistance to environment stresses, and nutrition quality by means of genetic manipulation. To obtain information on yield improvement applicable for the genetic engineering approach, we have attempted to dissect the molecular pathways that regulate organ size. We first isolated a putative homolog of ARGOS full-length cDNA from Chinese cabbage leaves, which we designated BrARGOS. At the transcription level, BrARGOS was detected in all organs tested in Chinese cabbage. To test the function of this gene, we then engineered Arabidopsis plants that would overexpres... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2852928 Tue, 29 Sep 2009 23:00:00 +0100 2852928 http://www.medworm.com/index.php?rid=2846585&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19784861%26dopt%3DAbstract Authors: Imamura T, Yasuda M, Kusano H, Nakashita H, Ohno Y, Kamakura T, Taguchi S, Shimada H Thanatin is an antimicrobial peptide with a strong and wide-ranging antimicrobial spectrum, including certain species of fungi and Gram-negative and Gram-positive bacteria. To evaluate the application of thanatin to the generation of disease-resistant plants, we introduced a synthetic thanatin gene into rice. Several transformants that expressed the introduced gene showed significant level of antimicrobial activity. The substances showing antimicrobial activity were partially purified from these transformants and their properties were determined. The molecule with characteristics similar to those of native thanatin on the elution pattern in HPLC analysis had an identical molecular mass to that... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2846585 Sat, 26 Sep 2009 23:00:00 +0100 2846585 http://www.medworm.com/index.php?rid=2821071&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19771527%26dopt%3DAbstract Authors: Anand S, Tyagi AK During differential screening of inflorescence-specific cDNA libraries from Oryza sativa indica, an arabinogalactan protein (OSIAGP) cDNA (586 bp) expressing preferentially in the inflorescence has been isolated. It encodes an arabinogalactan protein of 59 amino acids (6.4 kDa) with a transmembrane domain and a secretory domain at the N terminus. The protein shows homology with AGP23 from Arabidopsis, and its homologue in japonica rice is located on chromosome 6. OSIAGP transcripts also accumulate in shoots and roots of rice seedling grown in the dark, but light represses expression of the gene. Analysis of a genomic clone of OSIAGP revealed that its promoter contains several pollen-specificity and light-regulatory elements. The promoter confers pollen-prefer... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2821071 Mon, 21 Sep 2009 23:00:00 +0100 2821071 http://www.medworm.com/index.php?rid=2809859&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19763864%26dopt%3DAbstract Authors: Willenborg CJ, Brûlé-Babel AL, Van Acker RC Transgenic wheat (Triticum aestivum L.) with improved agronomic traits is currently being field-tested. Gene flow in space is well-documented, but isolation in time has not received comparable attention. Here, we report the results of a field experiment that investigated reductions in intraspecific gene flow associated with temporal isolation of flowering between T. aestivum conspecifics. Pollen-mediated gene flow (PMGF) between an imazamox-resistant (IR) volunteer wheat population and a non-IR spring wheat crop was assessed over a range of volunteer emergence timings and plant population densities that collectively promoted flowering asynchrony. Natural hybridization events between the two populations were detected by phen... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2809859 Thu, 17 Sep 2009 23:00:00 +0100 2809859 http://www.medworm.com/index.php?rid=2804452&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19757133%26dopt%3DAbstract Authors: Wolt JD, Keese P, Raybould A, Fitzpatrick JW, Burachik M, Gray A, Olin SS, Schiemann J, Sears M, Wu F Problem formulation is the first step in environmental risk assessment (ERA) where policy goals, scope, assessment endpoints, and methodology are distilled to an explicitly stated problem and approach for analysis. The consistency and utility of ERAs for genetically modified (GM) plants can be improved through rigorous problem formulation (PF), producing an analysis plan that describes relevant exposure scenarios and the potential consequences of these scenarios. A properly executed PF assures the relevance of ERA outcomes for decision-making. Adopting a harmonized approach to problem formulation should bring about greater uniformity in the ERA process for GM plants among regu... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2804452 Mon, 14 Sep 2009 23:00:00 +0100 2804452 http://www.medworm.com/index.php?rid=2734734&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19701794%26dopt%3DAbstract In this study, we have used a lentiviral delivery system to generate transgenic mice expressing altered levels (up or downregulated) of a gene of interest. Although this lentiviral-based approach led to high levels of transgenesis and germ line transmission, a wide variation in transgene expression was observed in most first and second generation mouse lines. In particular, despite the segregation of integrants into single-copy expressing mouse lines, transgene expression appeared to be the target of epigenetic regulatory mechanism, often causing the coexistence of high and low transgene expressing cells within a given tissue such as blood peripheral lymphocytes. The establishment and analysis of large number of mouse lines may therefore be required to select a stable transgenic line with ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2734734 Sat, 22 Aug 2009 23:00:00 +0100 2734734 http://www.medworm.com/index.php?rid=2717221&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19690973%26dopt%3DAbstract In this study, the effects of long-term, repeated exposure to transgenic papayas carrying the coat protein gene of Papaya ringspot virus and conventional non-transgenic papaya on the histology and selected biochemical parameters of the intestinal tract were compared. For 3 months, male and female Wistar rats received diets containing transgenic or non-transgenic papaya at twice the equivalent of the average daily consumption of fresh papayas. Gross and macroscopic appearance of intestinal tissues, as well as stomach tissues, was comparable (P < 0.05) as were total intestinal bacterial counts and activities of beta-glucuronidase. Activities of disaccharidases were not affected, neither were those of amylase (P < 0.05). Although significant differences were noted in the activity of Ca(... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2717221 Tue, 18 Aug 2009 23:00:00 +0100 2717221 http://www.medworm.com/index.php?rid=2717220&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19690974%26dopt%3DAbstract Authors: Olsson IA, Sandøe P The birth of the first transgenic primate to have inherited a transgene from its parents opens the possibility to set up transgenic marmoset colonies, as these monkeys are small and relatively easy to keep and breed in research facilities. The prospect of transgenic marmoset models of human disease, readily available in the way that transgenic laboratory mice are currently, prompts excitement in the scientific community; but the idea of monkeys being bred to carry diseases is also contentious. We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a d... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2717220 Tue, 18 Aug 2009 23:00:00 +0100 2717220 http://www.medworm.com/index.php?rid=2717219&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19690975%26dopt%3DAbstract Authors: Jha S, Chattoo BB Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2717219 Tue, 18 Aug 2009 23:00:00 +0100 2717219 http://www.medworm.com/index.php?rid=2717218&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19690976%26dopt%3DAbstract Authors: Coleman HD, Beamish L, Reid A, Park JY, Mansfield SD Nicotiana tabacum (tobacco) was transformed with three genes involved in sucrose metabolism, UDP-glucose pyrophosphorylase (UGPase, EC 2.7.7.9), sucrose synthase (SuSy, EC 2.4.1.13) and sucrose phosphate synthase (SPS, EC 2.4.1.14). Plants harbouring the single transgenes were subsequently crossed to produce double and triple transgenic lines, including: 2 x 35S::UGPase x SPS, 4CL::UGPase x SPS, 2 x 35S::SuSy x SPS, 4CL::SuSy x SPS, 2 x 35S::UGPase x SuSy x SPS, and 4CL::UGPase x SuSy x SPS. The ultimate aim of the study was to examine whether it is possible to alter cellulose production through the manipulation of sucrose metabolism genes. While altering sucrose metabolism using UGPase, SuSy and SPS does not have an end eff... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2717218 Tue, 18 Aug 2009 23:00:00 +0100 2717218 http://www.medworm.com/index.php?rid=2717217&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19690977%26dopt%3DAbstract Authors: van Roon M PMID: 19690977 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2717217 Tue, 18 Aug 2009 23:00:00 +0100 2717217 http://www.medworm.com/index.php?rid=2712459&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685153%26dopt%3DAbstract Authors: Gómez-Galera S, Rojas E, Sudhakar D, Zhu C, Pelacho AM, Capell T, Christou P Staple food crops, in particular cereal grains, are poor sources of key mineral nutrients. As a result, the world's poorest people, generally those subsisting on a monotonous cereal diet, are also those most vulnerable to mineral deficiency diseases. Various strategies have been proposed to deal with micronutrient deficiencies including the provision of mineral supplements, the fortification of processed food, the biofortification of crop plants at source with mineral-rich fertilizers and the implementation of breeding programs and genetic engineering approaches to generate mineral-rich varieties of staple crops. This review provides a critical comparison of the strategies that have been develope... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2712459 Fri, 14 Aug 2009 23:00:00 +0100 2712459 http://www.medworm.com/index.php?rid=2696347&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19669925%26dopt%3DAbstract Authors: Eppler E, Berishvili G, Mazel P, Caelers A, Hwang G, Maclean N, Reinecke M Several lines of GH-overexpressing fish have been produced and characterized concerning organ integrity, growth, fertility and health but few and contradictory data are available on IGF-I that mediates most effects of GH. Furthermore, nothing is known on IGF-II. Thus, the expression of both IGFs in liver and various extrahepatic sites of adult transgenic (GH-overexpressing) tilapia and age-matched wild-type fish was determined by real-time PCR. Both IGF-I and IGF-II mRNA were found in all organs investigated and were increased in gills, kidney, intestine, heart, testes, skeletal muscle and brain of the transgenics (IGF-I: 1.4-4-fold; IGF-II: 1.7-4.2-fold). Except for liver, brain and testis the increase... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2696347 Mon, 10 Aug 2009 23:00:00 +0100 2696347 http://www.medworm.com/index.php?rid=2696348&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19669584%26dopt%3DAbstract Authors: Hrytsenko O, Pohajdak B, Wright JR PMID: 19669584 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2696348 Fri, 07 Aug 2009 23:00:00 +0100 2696348 http://www.medworm.com/index.php?rid=2684307&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19662507%26dopt%3DAbstract Authors: Chatterjee S, Min L, Karuturi RK, Lufkin T A tissue-specific transgenic model was employed to test the effects of intron and vector sequences on transgene expression in zebrafish after microinjection. In this model, the 2.3 kb promoter taken from the 5' upstream region of the transcription initiation site of keratin 4 (krt4) was used to drive the enhanced green fluorescence protein (EGFP) reporter gene in a transgenic vector. For assaying the strength of EGFP expression, the effects of including an intron before the EGFP coding region or using different forms of DNA, including circular plasmid, linear full-length plasmid, and the linear transgene coding region without any prokaryotic vector sequence, were tested. After microinjection, the transgene expression was analyzed usin... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2684307 Thu, 06 Aug 2009 23:00:00 +0100 2684307 http://www.medworm.com/index.php?rid=2684306&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19662508%26dopt%3DAbstract Authors: Pouyet L, Carrier A Oxidative stress corresponds to an excess in reactive oxygen species (ROS) including free radicals which are highly reactive with cellular constituents. Thereby ROS induce damage to DNA, proteins and lipids, which are all involved in the etiology of numerous pathologies such as cancer. To prevent potential damage, a tight regulation of ROS level is achieved through numerous enzyme systems and small molecules such as glutathione and vitamin C. Mutant mouse models targeting antioxidant enzymes have confirmed their essential role in ROS level control, and have shown a limited redundancy of their activity. Additionally, a number of other mutant mouse models exhibit increased ROS levels, suggesting an antioxidant role for the corresponding targeted gene. This is... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2684306 Thu, 06 Aug 2009 23:00:00 +0100 2684306 http://www.medworm.com/index.php?rid=2674647&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19653112%26dopt%3DAbstract Authors: Penno CA, Kawabe Y, Ito A, Kamihira M We previously reported the production of human erythropoietin (hEpo) using genetically manipulated (GM) chickens. The recombinant hEpo was produced in the serum and egg white of the GM chickens, and the oligosaccharide chain structures of the serum-derived hEpo were more favorable than those of the egg white-derived hEpo. In the present study, a retroviral vector encoding an expression cassette for a fusion protein of hEpo and the Fc region of human immunoglobulin G (hEpo/Fc) was injected into developing chicken embryos, with the aim of recovering the serum-derived hEpo from egg yolk through the yolk accumulation mechanism of maternal antibodies. The GM chickens that hatched stably produced the hEpo/Fc fusion protein not only in their seru... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2674647 Mon, 03 Aug 2009 23:00:00 +0100 2674647 http://www.medworm.com/index.php?rid=2626777&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618287%26dopt%3DAbstract Authors: Conley AJ, Jevnikar AM, Menassa R, Brandle JE Plants have shown promise as bioreactors for the large-scale production of a wide variety of recombinant proteins. To increase the economic feasibility of this technology, numerous molecular approaches have been developed to enhance the production yield of these valuable proteins in plants. Alternatively, we chose to examine the temporal and spatial distribution of erythropoietin (EPO) accumulation during tobacco plant development, in order to establish the optimal harvesting time to further maximize heterologous protein recovery. EPO is used extensively worldwide for the treatment of anaemia and is currently the most commercially valuable biopharmaceutical on the market. Our results indicate that the concentration of recombinant E... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2626777 Fri, 17 Jul 2009 23:00:00 +0100 2626777 http://www.medworm.com/index.php?rid=2614486&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19609704%26dopt%3DAbstract Authors: Zurbrügg C, Hönemann L, Meissle M, Romeis J, Nentwig W The cultivation of genetically modified Bt maize has raised environmental concerns, as large amounts of plant residues remain in the field and may negatively impact the soil ecosystem. In a field experiment, decomposition of leaf residues from three genetically modified (two expressing the Cry1Ab, one the Cry3Bb1 protein) and six non-transgenic hybrids (the three corresponding non-transformed near-isolines and three conventional hybrids) was investigated using litterbags. To elucidate the mechanisms that cause differences in plant decomposition, structural plant components (i.e., C:N ratio, lignin, cellulose, hemicellulose) were examined. Furthermore, Cry1Ab and Cry3Bb1 protein concentrations in maize leaf residu... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2614486 Thu, 16 Jul 2009 23:00:00 +0100 2614486 http://www.medworm.com/index.php?rid=2591946&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19588264%26dopt%3DAbstract Authors: Colgan R, Atkinson CJ, Paul M, Hassan S, Drake PM, Sexton AL, Santa-Cruz S, James D, Hamp K, Gutteridge C, Ma JK Nicotiana tabacum is emerging as a crop of choice for production of recombinant protein pharmaceuticals. Although there is significant commercial expertise in tobacco farming, different cultivation practices are likely to be needed when the objective is to optimise protein expression, yield and extraction, rather than the traditional focus on biomass and alkaloid production. Moreover, pharmaceutical transgenic tobacco plants are likely to be grown initially within a controlled environment, the parameters for which have yet to be established. Here, the growth characteristics and functional recombinant protein yields for two separate transgenic tobacco plant lines wer... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2591946 Wed, 08 Jul 2009 23:00:00 +0100 2591946 http://www.medworm.com/index.php?rid=2585086&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19585264%26dopt%3DAbstract Authors: Michaud JL, Stitt-Cavanaugh E, Endlich N, Endlich K, De Repentigny Y, Kothary R, Kennedy CR Mutations in the gene ACTN4 encoding the actin bundling protein-alpha-actinin-4 underlie an inherited form of kidney lesions known as focal segmental glomerulosclerosis (FSGS). Previously, we developed a model for this condition by generating mice with podocyte-specific overexpression of a disease-causing mutant alpha-actinin-4 (K256E-ACTN4 (pod+)). However, whether alpha-actinin-4 overexpression artifacts and not the gain of affinity effects of the mutation accounted for the robust FSGS phenotype in these mice was unclear. To address this question, we developed a control line of mice with podocyte-specific overexpression of wildtype alpha-actinin-4 (wt-ACTN4 (pod+)). An 8.3 kb fragment... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2585086 Tue, 07 Jul 2009 23:00:00 +0100 2585086 http://www.medworm.com/index.php?rid=2585087&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19582587%26dopt%3DAbstract Authors: Cheng B, Wu G, Vrinten P, Falk K, Bauer J, Qiu X Eicosapentaenoic acid (EPA, 20:5n-3) plays an important role in many aspects of human health. In our efforts towards producing high levels of EPA in plants, we investigated the effects of different host species, genes and promoters on EPA biosynthesis. Zero-erucic acid Brassica carinata appeared to be an outstanding host species for EPA production, with EPA levels in transgenic seed of this line reaching up to 25%. Two novel genes, an 18-carbon omega3 desaturase (CpDesX) from Claviceps purpurea and a 20-carbon omega3 desaturase (Pir-omega3) from Pythium irregulare, proved to be very effective in increasing EPA levels in high-erucic acid B. carinata. The conlinin1 promoter from flax functioned reasonably well in B. carinata, and ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2585087 Mon, 06 Jul 2009 23:00:00 +0100 2585087 http://www.medworm.com/index.php?rid=2571890&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19572206%26dopt%3DAbstract Authors: Xiang ZG, Jiang DD, Liu Y, Zhang LF, Zhu LP In order to study the biological significance of alpha-mannosidase Man2c1, hMan2c1 transgenic mice were developed. In 113 F0 mice, eight were found to be genomic PCR positive for hMan2c1; 9/20 (45%) F1 mice, 16/21 (76.2%) F2 mice, and 12/14 (85.7%) F3 mice were genomic PCR positive for hMan2c1. RT-PCR demonstrated hMan2c1 mRNA transcription in four of eight transgenic lines. Enzymatic activity on p-nitrophenyl-alpha-D: -mannopyranoside was enhanced in 35# and 54# transgenic mice and real-time RT-PCR showed hMan2c1 mRNA expression in these mice. Reduced Con A binding to splenocytes implied N-glycosylation modification of host proteins by hMan2c1 transgene. hMan2c1 transgene promoted growth, invasion, and metastasis to lung of implante... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2571890 Wed, 01 Jul 2009 23:00:00 +0100 2571890 http://www.medworm.com/index.php?rid=2562245&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19568949%26dopt%3DAbstract Authors: Guo JC, Duan RJ, Hu XW, Li KM, Fu SP This research reports a promising approach to increase a plant's physiological cytokinin content. This approach also enables the increase to play a role in plant growth and development by introducing the ipt gene to downstream transcriptionally fuse with other genes under the control of a CaMV35S promoter, in which the ipt gene is far from the 35S promoter. According to Kozak's ribosome screening model, expression of the ipt gene is reduced by the terminal codon of the first gene and the internal untranslated nucleotides between the fused genes. In the transgenic plants pVKH35S-GUS-ipt, pVKH35S-AOC-ipt, and pVKH35S-AtGolS2-ipt, cytokinins were increased only two to threefold, and the plants grew more vigorously than the pVKH35S-AOC or pVKH3... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2562245 Tue, 30 Jun 2009 23:00:00 +0100 2562245 http://www.medworm.com/index.php?rid=2562246&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19565349%26dopt%3DAbstract Authors: Rauschen S PMID: 19565349 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2562246 Mon, 29 Jun 2009 23:00:00 +0100 2562246 http://www.medworm.com/index.php?rid=2556531&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19562498%26dopt%3DAbstract Authors: Singh AK, Verma SS, Bansal KC We have developed a method for plastid transformation in eggplant (Solanum melongena L.), a solanaceous plant species. Plastid transformation in eggplant was achieved by bombardment of green stem segments with pPRV111A plastid expression vector carrying the aadA gene encoding aminoglycoside 3''-adenylyltransferase. Biolistic delivery of the pPRV111A plasmid yielded transplastomic plants at a frequency of two per 21 bombarded plates containing 25 stem explants each. Integration of the aadA gene in the plastome was verified by PCR analysis and also by Southern blotting using 16S rDNA (targeting sequence) and the aadA gene as a probe. Transplastomic expression of the aadA gene was verified by RT-PCR. The development of transplastomic technology in eg... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2556531 Sat, 27 Jun 2009 23:00:00 +0100 2556531 http://www.medworm.com/index.php?rid=2543100&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19557540%26dopt%3DAbstract Authors: Kawazu Y, Fujiyama R, Noguchi Y, Kubota M, Ito H, Fukuoka H Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce line was conducted by comparing it with the parental, non-transformed 'Kaiser' cultivar. There were no significant differences between transgenic and non-transgenic lettuce in terms of pollen fertility, pollen dispersal, seed production, seed dispersal, dormancy, germination, growth of seedlings under low or high temperature, chromatographic patterns of leaf extracts, or effects of lettuce on the growth of ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543100 Thu, 25 Jun 2009 23:00:00 +0100 2543100 http://www.medworm.com/index.php?rid=2543102&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19548100%26dopt%3DAbstract We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varieties, namely two scientific papers describing laboratory force-feeding trials on ladybirds and daphnia, and previous data on Lepidoptera, aquatic and soil organisms. We demonstrate that the suspension is based on an incomplete list of references, ignores the widely admitted case-by-case approach, and confuses potential hazard and proven risk in the scientific procedure of risk assessment. Furthermore, we did not find any justification for this suspension in our extensive survey o... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543102 Mon, 22 Jun 2009 23:00:00 +0100 2543102 http://www.medworm.com/index.php?rid=2543101&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19548101%26dopt%3DAbstract Authors: Praveen S, Ramesh SV, Mishra AK, Koundal V, Palukaitis P We investigated viral gene suppression in an infected tomato, by transforming it with RNA inhibition (RNAi) constructs derived from same viral gene. To develop RNAi constructs, conserved sequences ranging from 21 to 200 nt of the viral target AC4 gene of various viruses causing the tomato leaf curl disease were chosen. The double-stranded (ds)RNA producing constructs carry the sense and antisense portions of these sequences and are separated by different introns behind a constitutive promoter. We compared the levels of suppression of the viral target gene by transforming four different RNAi constructs with varied arm length of dsRNA. Gene silencing levels of the viral target gene were found to be directly proportional to... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543101 Mon, 22 Jun 2009 23:00:00 +0100 2543101 http://www.medworm.com/index.php?rid=2543103&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19544083%26dopt%3DAbstract Authors: Obro J, Borkhardt B, Harholt J, Skjøt M, Willats WG, Ulvskov P Despite the wide occurrence of pectin in nature only a few source materials have been used to produce commercial pectins. One of the reasons for this is that many plant species contain pectins with high levels of neutral sugar side chains or that are highly substituted with acetyl or other groups. These modifications often prevent gelation, which has been a major functional requirement of commercial pectins until recently. We have previously shown that modification of pectin is possible through heterologous expression of pectin degrading enzymes in planta. To test the effect of simultaneous modification of the two main neutral pectic side chains in pectic rhamnogalacturonan I (RGI), we constitutively expressed... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543103 Sat, 20 Jun 2009 23:00:00 +0100 2543103 http://www.medworm.com/index.php?rid=2543104&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19544002%26dopt%3DAbstract Authors: Wen XP, Ban Y, Inoue H, Matsuda N, Moriguchi T To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European pear (Pyrus communis L. 'Ballad') line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl(2), PbCl(2), ZnCl(2), or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543104 Fri, 19 Jun 2009 23:00:00 +0100 2543104 http://www.medworm.com/index.php?rid=2543105&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19543801%26dopt%3DAbstract Authors: Vogler U, Rott AS, Gessler C, Dorn S While most risk assessments contrast a transgenic resistant to its isogenic line, an additional comparison between the transgenic line and a classically bred cultivar with the same resistance gene would be highly desirable. Our approach was to compare headspace volatiles of transgenic scab resistant apple plants with two representative cultivars (the isogenic 'Gala' and the scab resistance gene-containing 'Florina'). As modifications in volatile profiles have been shown to alter plant relationships with non-target insects, we analysed headspace volatiles from apple plants subjected to different infection types by gas chromatography-mass spectrometry. Marked differences were found between healthy and leafminer (Phyllonorycter blancardella) i... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543105 Thu, 18 Jun 2009 23:00:00 +0100 2543105 http://www.medworm.com/index.php?rid=2543107&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19533404%26dopt%3DAbstract Authors: Zhao A, Zhao T, Zhang Y, Xia Q, Lu C, Zhou Z, Xiang Z, Nakagaki M We constructed three different fibroin H-chain expression systems to estimate the efficacy of producing recombinant proteins in the cocoon of transgenic silkworms. The results showed that the three different EGFP/H-chain fusion genes were all expressed selectively in the posterior silk gland of the transgenic silkworm. The recombinant protein content of transgenic silkworm cocoons is up to 15% (w/w) when using the most highly efficient H-chain expression system. To our knowledge, in comparison with silkworm silk gland expression systems in the literature, the highly efficient expression system developed in this study is the most efficient silkworm silk gland expression system to date. This expression system is t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543107 Tue, 16 Jun 2009 23:00:00 +0100 2543107 http://www.medworm.com/index.php?rid=2543106&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19533405%26dopt%3DAbstract Authors: Bellocchi G, Bertholet V, Hamels S, Moens W, Remacle J, Van den Eede G This paper illustrates the advantages that a fuzzy-based aggregation method could bring into the validation of a multiplex method for GMO detection (DualChip((R)) GMO kit, Eppendorf). Guidelines for validation of chemical, bio-chemical, pharmaceutical and genetic methods have been developed and ad hoc validation statistics are available and routinely used, for in-house and inter-laboratory testing, and decision-making. Fuzzy logic allows summarising the information obtained by independent validation statistics into one synthetic indicator of overall method performance. The microarray technology, introduced for simultaneous identification of multiple GMOs, poses specific validation issues (patterns of perfor... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543106 Mon, 15 Jun 2009 23:00:00 +0100 2543106 http://www.medworm.com/index.php?rid=2543108&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19526355%26dopt%3DAbstract Authors: Fan MJ, Chen S, Kung YJ, Cheng YH, Bau HJ, Su TT, Yeh SD The commercially valuable transgenic papaya lines carrying the coat protein (CP) gene of Papaya ringspot virus (PRSV) and conferring virus resistance have been developed in Hawaii and Taiwan in the past decade. Prompt and sensitive protocols for transgene-specific and event-specific detections are essential for traceability of these lines to fulfill regulatory requirement in EU and some Asian countries. Here, based on polymerase chain reaction (PCR) approaches, we demonstrated different detection protocols for characterization of PRSV CP-transgenic papaya lines. Transgene-specific products were amplified using different specific primer pairs targeting the sequences of the promoter, the terminator, the selection marker, a... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543108 Sat, 13 Jun 2009 23:00:00 +0100 2543108 http://www.medworm.com/index.php?rid=2543109&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19521792%26dopt%3DAbstract Authors: Howard PW, Howard TL, Maurer RA Ift172 encodes a gene product that is part of a complex that mediates intraflagellar transport (IFT), a process necessary for the genesis and maintenance of cilia. Genetic studies in mice have offered evidence that Ift172 also plays a role in hedgehog signaling. Disruption of Ift172 in mice is associated with lethality at about embryonic day 11, limiting studies to understand the role for Ift172 in later development and the adult. To further our understanding of the later roles of Ift172, we have generated mice with a conditional allele for Ift172. We have confirmed the phenotype of the disrupted allele by using CRE expression directed by the prx1 enhancer to disrupt the conditional Ift172 allele in the developing limb. PMID: 19521792 [PubMe... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543109 Thu, 11 Jun 2009 23:00:00 +0100 2543109 http://www.medworm.com/index.php?rid=2543110&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19507046%26dopt%3DAbstract Authors: Gambino G, Perrone I, Carra A, Chitarra W, Boccacci P, Torello Marinoni D, Barberis M, Maghuly F, Laimer M, Gribaudo I Eight transgenic grapevine lines transformed with the coat protein gene of Grapevine fanleaf virus (GFLV-CP) were analyzed for a correlation between transgene expression, siRNAs production and DNA methylation. Bisulphite genome sequencing was used for a comprehensive analysis of DNA methylation. Methylated cytosine residues of CpG and CpNpG sites were detected in the GFLV-CP transgene, in the T7 terminator and in the 35S promoter of three grapevines without transgene expression, but no detectable level of siRNAs was recorded in these lines. The detailed analysis of 8 lines revealed the complex arrangements of T-DNA and integrated binary vector sequences as cru... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543110 Mon, 08 Jun 2009 23:00:00 +0100 2543110 http://www.medworm.com/index.php?rid=2543111&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19504348%26dopt%3DAbstract Authors: Notini AJ, Li R, Western PS, Sinclair AH, White SJ The ability to rapidly and unequivocally distinguish heterozygous from homozygous transgenic mice is an integral part of any breeding strategy. Here we describe a quick and simple protocol for determining the zygosity of transgenic mice at multiple loci in a single reaction. This involved the development of a multiplex ligation-dependent probe amplification (MLPA) probe mix to simultaneously measure common transgenic alleles such as Cre recombinase (Cre), neomycin (Neo), beta-galactosidase (LacZ) and enhanced green fluorescent protein (eGFP), as well as loci specific to the X and Y chromosome to allow sexing. Each reaction required as little as 100 ng of genomic DNA isolated from a tail biopsy using a simple procedure. Normali... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543111 Sat, 06 Jun 2009 23:00:00 +0100 2543111 http://www.medworm.com/index.php?rid=2543112&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19504171%26dopt%3DAbstract In this study, we have established an efficient plant bioreactor platform for mass production of rhIGFBP-3. Different expression constructs, driven by the seed-specific phaseolin promoter, were designed and transformed into tobacco plant via Agrobacterium. To enhance protein expression level, the signal peptide (SP) and the C-terminal tetrapeptide AFVY of phaseolin were used to direct rhIGFBP-3 to protein storage vacuole (PSV) in tobacco seed for stable accumulation. Western blot analysis showed that rhIGFBP-3 was successfully synthesized in transgenic tobacco seeds, with the highest protein expression of 800 mug/g dry weight. The localization of rhIGFBP-3 in PSV was also evident by confocal immunofluorescence microscopy. Our results indicated that protein sorting sequences could benefit t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543112 Fri, 05 Jun 2009 23:00:00 +0100 2543112 http://www.medworm.com/index.php?rid=2543113&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19484500%26dopt%3DAbstract Authors: Kvell K, Czömpöly T, Hiripi L, Balogh P, Kóbor J, Bodrogi L, Pongrácz JE, Ritchie WA, Bősze Z Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1alpha promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth gene... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543113 Sat, 30 May 2009 23:00:00 +0100 2543113 http://www.medworm.com/index.php?rid=2543116&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479337%26dopt%3DAbstract Authors: Kim MJ, Lyu J, Sohn KB, Kim M, Cho MC, Joo CK, Lee K Inhibition of Na,K-ATPase causes opacification of the lens through abnormal increases in sodium and calcium levels, disturbed osmotic equilibrium, activation of proteolytic enzymes and cell damage. We previously identified Translationally Controlled Tumor Protein (TCTP) as a cytoplasmic repressor of Na,K-ATPase and confirmed that systemic hypertension is induced in transgenic mice over-expressing TCTP through inhibition of vascular Na,K-ATPase and increased intracellular calcium mobilization. In the current study, we confirmed the role of TCTP in causing intracellular calcium mobilization by inhibiting Na,K-ATPase in a human lens epithelial cell line and further showed that some of the TCTP-transgenic mice develop cataracts ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543116 Thu, 28 May 2009 23:00:00 +0100 2543116 http://www.medworm.com/index.php?rid=2543115&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479338%26dopt%3DAbstract Authors: Rojas-Anaya E, Loza-Rubio E, Olivera-Flores MT, Gomez-Lim M Antigens derived from various pathogens can readily be synthesized at high levels in plants in their authentic forms. Such antigens administered orally can induce an immune response and, in some cases, result in protection against a subsequent challenge. We here report the expression of rabies virus G protein into carrots. The G gene was subcloned into the pUCpSSrabG vector and then used to transform carrot embryogenic cells by particle bombardment. The carrot cells were selected in liquid medium, a method previously unreported. The presence of the transgene was verified by PCR, and by RT-PCR. By western blot, G protein transgene was identified in 93.3% of adult carrot roots. The G protein was quantified by densitomet... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543115 Thu, 28 May 2009 23:00:00 +0100 2543115 http://www.medworm.com/index.php?rid=2543114&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479339%26dopt%3DAbstract Authors: Inui H, Sasaki H, Chua NH, Ohkawa H Transgenic Arabidopsis plants carrying a recombinant human estrogen receptor gene and a green fluorescent protein reporter gene were used to bioassay estrogenic compounds. We constructed four recombinant human estrogen receptor genes by combining the DNA-binding domain of LexA, a synthetic nuclear localization signal, a ligand-binding domain of the human estrogen receptor, and a transactivation domain of VP16 in different orders; the XEV plants were the most sensitive, and were able to detect 0.001 ng ml(-1) of 17ss-estradiol (E(2)). The transgenic plants absorbed E(2) and 4-nonylphenol present in the nutrient solution, whereas most of the other compounds seemed to be retained in, or on, the roots. Estrone, methoxychlor, bisphenol A, 4-nonyl... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543114 Thu, 28 May 2009 23:00:00 +0100 2543114 http://www.medworm.com/index.php?rid=2543117&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19475495%26dopt%3DAbstract Authors: Cederberg A, Grände M, Rhedin M, Peng XR, Enerbäck S We have developed a generic model for in vitro high-throughput screening for agents regulating transcription of genes in the mouse genome here exemplified by Foxc2, a forkhead transcription factor involved in regulation of adipocyte metabolism. We made a Foxc2-LacZ reporter "knock-in" mouse in which one of the two Foxc2 alleles has been inactivated and replaced by a LacZ reporter gene. Mouse embryonic fibroblasts, derived from such mice, were differentiated in vitro to adipocytes and used in cell-based screens. Forskolin as well as 12-O-tetradecanoylphorbol-13-acetate (TPA) increased levels of Foxc2nLacZ fusion protein. We could also demonstrate that this was paralleled by an increase in Foxc2 mRNA, transcribed fro... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543117 Wed, 27 May 2009 23:00:00 +0100 2543117 http://www.medworm.com/index.php?rid=2543119&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19468852%26dopt%3DAbstract Authors: Zhang J, Zou L, Liu Q, Li J, Zhou J, Wang Y, Li N, Liu T, Wei H, Wu M, Wan Y, Wu Y Dendritic cell (DC) specific transgenic mice are a most important model for investigating dendritic cell functions in vivo. Recently, lentivirus mediated gene transfer has become a powerful and convenient method for generation of transgenic mice. We cloned a 1.2 kb CD11c promoter and constructed a lentiviral vector, which efficiently drove DC-specific expression in vitro. After microinjection of purified virus into the perivitelline space of single-cell embryo, more than 80% newborn mice were transgenic and 7 F0 founders were rapidly generated in 2 months. GFP was strictly expressed in CD11c+ cells in spleens, thymus and lymph nodes of the transgenic mice. Importantly, the physiological characte... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543119 Mon, 25 May 2009 23:00:00 +0100 2543119 http://www.medworm.com/index.php?rid=2543118&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19468853%26dopt%3DAbstract Authors: Montoliu L PMID: 19468853 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543118 Mon, 25 May 2009 23:00:00 +0100 2543118 http://www.medworm.com/index.php?rid=2543120&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19466576%26dopt%3DAbstract Authors: Landi L, Capocasa F, Costantini E, Mezzetti B The potential to improve strawberry cultivation was assessed regarding the use the rolC genes from Agrobacterium rhizogenes that can confer higher levels of free cytokinins. Strawberry (cv. Calypso) rolC lines were produced by genetic transformation of Agrobacterium tumefaciens. Yield and fruit quality of the control and transgenic lines were measured under open-field conditions. The effects of the transgenic rolC lines depended on gene copy number: rolC lines with one (Line A) or two gene (Line B) copies showed 30% greater yields than controls, due to 20% more fruit per plant and an increased fruit weight. Line A also differed in terms of the highest fruit quality, due to 10.5% increased soluble solids and 12.7% higher acidity. Mo... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543120 Fri, 22 May 2009 23:00:00 +0100 2543120 http://www.medworm.com/index.php?rid=2543121&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19434509%26dopt%3DAbstract In this study, GAD2DeltaC, under the control of the rice glutelin promoter (GluB-1), was introduced into rice cells via Agrobacterium-mediated transformation to produce transgenic rice lines. Analysis of the free amino acid content of rice grains revealed up to about a 30-fold higher level of GABA than in non-transformed rice grains. There were also very high levels of various free protein amino acids in the seeds. GABA-enriched rice grains were milled to a fine powder for oral administration to spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto rats (WKYs). Six weeks of administration showed that transgenic rice brings about a 20 mmHg decrease in blood pressure in two different kinds of SHRs, while there was no significant hypotensive effect in WKYs. These results sugges... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543121 Mon, 11 May 2009 23:00:00 +0100 2543121 http://www.medworm.com/index.php?rid=2543122&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19418238%26dopt%3DAbstract Authors: Li W, Kotoshiba S, Kaldis P Early studies on cell cycle regulation were based on experiments in model systems (Yeast, Xenopus, Starfish, Drosophila) and have shaped the way we understand many events that control the cell cycle. Although these model systems are of great value, the last decade was highlighted by studies done in human cells and using in vivo mouse models. Mouse models are irreplaceable tools for understanding the genetics, development, and survival strategies of mammals. New developments in generating targeting vectors and mutant mice have improved our approaches to study cell cycle regulation and cancer. Here we summarize the most recent advances of mouse model approaches in dissecting the mechanisms of cell cycle regulation and the relevance to human disease. ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543122 Tue, 05 May 2009 23:00:00 +0100 2543122 http://www.medworm.com/index.php?rid=2543123&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19415521%26dopt%3DAbstract In conclusion, genetically engineered tomato showed no toxicity in mice and allergenicity is similar to the wild-type tomato. PMID: 19415521 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543123 Mon, 04 May 2009 23:00:00 +0100 2543123 http://www.medworm.com/index.php?rid=2543124&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19412740%26dopt%3DAbstract In conclusion, this unique Tg(k18:shh:RFP) fish line, should be an excellent experimental animal for screening for a lower toxicity level of the new Hh-inhibitor and can even be used as a new anti-cancer drug-screening platform. PMID: 19412740 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543124 Sat, 02 May 2009 23:00:00 +0100 2543124 http://www.medworm.com/index.php?rid=2543126&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19404763%26dopt%3DAbstract Authors: L'hostis-Guidet A, Recher G, Guillet B, Al-Mohammad A, Coumailleau P, Tiaho F, Boujard D, Madigou T Combining two existing protocols of trangenesis, namely the REMI and the I-SceI meganuclease methods, we generated Xenopus leavis expressing a transgene under the control of a promoter that presented a restricted pattern of activity and a low level of expression. This was realized by co-incubating sperm nuclei, the I-SceI enzyme and the transgene prior to transplantation into unfertilized eggs. The addition of the woodchuck hepatitis virus posttranscriptional regulatory element in our constructs further enhanced the expression of the transgene without affecting the tissue-specificity of the promoter activity. Using this combination of methods we produced high rates of fully tran... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543126 Wed, 29 Apr 2009 23:00:00 +0100 2543126 http://www.medworm.com/index.php?rid=2543125&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19404764%26dopt%3DAbstract Authors: Wang T, Shi Y, Li Y, Darmency H Is there any risk that the threshold for admixture of genetically modified seeds in the harvest of a conventional cultivar, 0.9% in Europe, will be exceeded in the case of inbreeder crops? Using herbicide-resistant foxtail millet, Setaria italica, as a model of a preferentially autogamous crop, such as wheat and rice, field experiments show that genotype admixture due to pollen flow between adjacent fields is about 0.03% on average for the 10 adjacent meters, and 10 times less in the next 20-m lane. In the case of a maternally inherited resistance gene, the admixture rate is at least 100 times lower. Recessive herbicide resistance has also been tested but would be efficient only if the agreed coexistence rules were based on phenotype detection. ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543125 Tue, 28 Apr 2009 23:00:00 +0100 2543125 http://www.medworm.com/index.php?rid=2543129&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19396621%26dopt%3DAbstract Authors: Van Keuren ML, Gavrilina GB, Filipiak WE, Zeidler MG, Saunders TL Transgenic mice are widely used in biomedical research to study gene expression, developmental biology, and gene therapy models. Bacterial artificial chromosome (BAC) transgenes direct gene expression at physiological levels with the same developmental timing and expression patterns as endogenous genes in transgenic animal models. We generated 707 transgenic founders from 86 BAC transgenes purified by three different methods. Transgenesis efficiency was the same for all BAC DNA purification methods. Polyamine microinjection buffer was essential for successful integration of intact BAC transgenes. There was no correlation between BAC size and transgenic rate, birth rate, or transgenic efficiency. A narrow DNA con... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543129 Sat, 25 Apr 2009 23:00:00 +0100 2543129 http://www.medworm.com/index.php?rid=2543127&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19396622%26dopt%3DAbstract Authors: Coll A, Nadal A, Collado R, Capellades G, Messeguer J, Melé E, Palaudelmàs M, Pla M Maize is a major food crop and genetically modified (GM) varieties represented 24% of the global production in 2007. Authorized GM organisms have been tested for human and environmental safety. We previously used microarrays to compare the transcriptome profiles of widely used commercial MON810 versus near-isogenic varieties and reported differential expression of a small set of sequences in leaves of in vitro cultured plants of AristisBt/Aristis and PR33P67/PR33P66 (Coll et al. 2008). Here we further assessed the significance of these differential expression patterns in plants grown in a real context, i.e. in the field. Most sequences that were differentially expressed in plants cult... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543127 Sat, 25 Apr 2009 23:00:00 +0100 2543127 http://www.medworm.com/index.php?rid=2543133&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19396561%26dopt%3DAbstract Authors: Gursky Y, Bibilashvili R, Minashkin M, Krasnov A, Deikin A, Ermolkevich T, Popov A, Verbovaya L, Rutkevich N, Shevelev A, Georgieva S, Razin SV, Goldman I, Sadchikova E Human pro-urokinase expressed in the mammary glands of transgenic animals is quickly activated and converted to urokinase by proteases that are present in the milk. Thus, it is nearly impossible to isolate full-sized pro-urokinase from the milk of transgenic animals. To solve this problem, we constructed transgenic mice that express human pro-urokinase and modified ecotin, which is a potent serine protease inhibitor from E. coli, in their mammary glands. The gene encoding ecotin was modified so as to enhance its specificity for the human urokinase-type plasminogen activator. Co-expression of modified ecotin and... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543133 Fri, 24 Apr 2009 23:00:00 +0100 2543133 http://www.medworm.com/index.php?rid=2543131&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19396562%26dopt%3DAbstract Authors: Li XG, Liu B, Heia S, Liu DD, Han ZM, Zhou KX, Cui JJ, Luo JY, Zheng YP The attenuation of disease resistance in transgenic insect-resistant cotton has become one of the important factors restricting cotton production in China. Two transgenic insect-resistant cotton lines and their parental conventional cotton lines were used as the testing materials. The effects of root exudates of these cotton lines on the spore germination and mycelial growth of Fusarium oxysporum were studied and the components, contents of amino acids and sugars were determined. The results showed that the resistance of the two insect-resistant cotton lines to F. oxysporum was inferior to the parental lines, and that their root exudates promoted fungal spore germination and mycelial growth. Considerable d... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543131 Fri, 24 Apr 2009 23:00:00 +0100 2543131 http://www.medworm.com/index.php?rid=2543137&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19387858%26dopt%3DAbstract Authors: Garigliany MM, Cloquette K, Leroy M, Decreux A, Goris N, De Clercq K, Desmecht D Mx proteins are interferon-induced members of the dynamin superfamily of large guanosine triphosphatases. These proteins have attracted much attention because some display antiviral activity against pathogenic RNA viruses, such as members of the orthomyxoviridae, bunyaviridae, and rhabdoviridae families. Among the diverse mammalian Mx proteins examined so far, we have recently demonstrated in vitro that the Bos taurus isoform 1 (boMx1) is endowed with exceptional anti-rabies-virus activity. This finding has prompted us to seek an appropriate in vivo model for confirming and evaluating gene therapy strategies. Using a BAC transgene, we have generated transgenic mouse lines expressing the antiviral ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543137 Wed, 22 Apr 2009 23:00:00 +0100 2543137 http://www.medworm.com/index.php?rid=2543135&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19387859%26dopt%3DAbstract Authors: Willenborg CJ, Brûlé-Babel AL, Van Acker RC Transgenic wheat is currently being field tested with the intent of eventual commercialization. The development of wheat genotypes with novel traits has raised concerns regarding the presence of volunteer wheat populations and the role they may play in facilitating transgene movement. Here, we report the results of a field experiment that investigated the potential of spring wheat plant population density and crop height to minimize gene flow from a herbicide-resistant (HR) volunteer population to a non-HR crop. Pollen-mediated gene flow (PMGF) between the HR volunteer wheat population and four conventional spring wheat genotypes varying in height was assessed over a range of plant population densities. Natural hybridizatio... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543135 Tue, 21 Apr 2009 23:00:00 +0100 2543135 http://www.medworm.com/index.php?rid=2543139&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19381853%26dopt%3DAbstract Authors: Lowe BA, Shiva Prakash N, Way M, Mann MT, Spencer TM, Boddupalli RS Transgene copy number is an important criterion for determining the utility of transgenic events. Single copy integration events are highly desirable when the objective is to produce marker free plants through segregation or when it is necessary to introgress different transgenes into commercial cultivars from different transgenic events. In contrast multi-copy events are advocated by several authors for higher expression of the transgene. Till recently, it was thought that employment of the particle gun for transformation results in the production of a high proportion of multi-copy events often with complex integration pattern when compared to Agrobacterium-mediated transformation. However, it has been demons... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2543139 Mon, 20 Apr 2009 23:00:00 +0100 2543139 http://www.medworm.com/index.php?rid=2320985&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19357984%26dopt%3DAbstract Authors: Rai M, He C, Wu R To identify minimal effective promoters for driving abiotic stress-inducible transgene expression in rice, we selected promoter elements of three stress-responsive genes, viz. rab16A coding for dehydrin, OsABA2 coding for zeaxanthin epoxidase, and a gene coding for a hypothetical protein (HP1) based on the presence of ABA-, salt- and drought-responsive cis-acting elements. These were translationally fused to the gusA reporter gene and introduced into rice to study their effect on heterologous gene expression. The OsABA2 promoter was found to be the most effective and desirable promoter among the three in terms of driving a low constitutive transgene expression under normal conditions and high induction in response to ABA, salt and drought stress, the highest ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320985 Thu, 09 Apr 2009 04:00:00 +0100 2320985 http://www.medworm.com/index.php?rid=2320983&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19357985%26dopt%3DAbstract Authors: Umeyama K, Watanabe M, Saito H, Kurome M, Tohi S, Matsunari H, Miki K, Nagashima H Pigs have been recognized as an excellent biomedical model for investigating a variety of human health issues. We developed genetically modified pigs that exhibit the apparent symptoms of diabetes. Transgenic cloned pigs carrying a mutant human hepatocyte nuclear factor 1alpha gene, which is known to cause the type 3 form of maturity-onset diabetes of the young, were produced using a combined technology of intracytoplasmic sperm injection-mediated gene transfer and somatic cell nuclear transfer. Although most of the 22 cloned offspring obtained died before weaning, four pigs that lived for 20-196 days were diagnosed as diabetes mellitus with nonfasting blood glucose levels greater than 200 mg/dl... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320983 Thu, 09 Apr 2009 04:00:00 +0100 2320983 http://www.medworm.com/index.php?rid=2320981&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19357986%26dopt%3DAbstract Authors: Xiao L, Lu C, Zhang B, Bo H, Wu Y, Wu G, Cao Y, Yu D Gene transferability from transgenic rapeseed to various subspecies and varieties of Brassica rapa was assessed in this study. Artificial crossability was studied in 118 cultivars of 7 B. rapa subspecies and varieties with the transgenic rapeseed GT73 (Brassica napus) as the pollen donor. On average 5.7 seeds were obtained per pollination, with a range from 0.05 to 19.4. The heading type of B. rapa L. showed significantly higher crossability than non-heading types of B. rapa. The spontaneous outcrossing rate between B. rapa (female) and the transgenic rapeseed Ms8 x Rf3 (B. napus) (male) ranged from 0.039 to 0.406%, with an average of 0.19%. The fertilization process and the development of the hybrid seeds as shown by fluore... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320981 Thu, 09 Apr 2009 04:00:00 +0100 2320981 http://www.medworm.com/index.php?rid=2320978&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19357987%26dopt%3DAbstract Authors: Shelton AM, Naranjo SE, Romeis J, Hellmich RL, Wolt JD, Federici BA, Albajes R, Bigler F, Burgess EP, Dively GP, Gatehouse AM, Malone LA, Roush R, Sears M, Sehnal F PMID: 19357987 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320978 Thu, 09 Apr 2009 04:00:00 +0100 2320978 http://www.medworm.com/index.php?rid=2320987&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19353301%26dopt%3DAbstract Authors: Fouad WM, Altpeter F Metabolic engineering for beta-alanine over-production in plants is expected to enhance environmental stress tolerance. The Escherichia coli L: -aspartate-alpha-decarboxylase (AspDC) encoded by the panD gene, catalyzes the decarboxylation of L: -aspartate to generate beta-alanine and carbon dioxide. The constitutive E. coli panD expression cassette was co-introduced with the constitutive, selectable aadA expression cassette into the chloroplast genome of tobacco via biolistic gene transfer and homologous recombination. Site specific integration of the E. coli panD expression cassette into the chloroplast genome and generation of homotransplastomic plants were confirmed by PCR and Southern blot analysis, respectively, following plant regeneration and germin... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320987 Wed, 08 Apr 2009 04:00:00 +0100 2320987 http://www.medworm.com/index.php?rid=2320989&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19347596%26dopt%3DAbstract Authors: Haga Y, Dominique VJ, Du SJ To characterize the process of vertebral segmentation and disc formation in living animals, we analyzed tiggy-winkle hedgehog (twhh):green fluorescent protein (gfp) and sonic hedgehog (shh):gfp transgenic zebrafish models that display notochord-specific GFP expression. We found that they showed distinct patterns of expression in the intervertebral discs of late stage fish larvae and adult zebrafish. A segmented pattern of GFP expression was detected in the intervertebral disc of twhh:gfp transgenic fish. In contrast, little GFP expression was found in the intervertebral disc of shh:gfp transgenic fish. Treating twhh:gfp transgenic zebrafish larvae with exogenous retinoic acid (RA), a teratogenic factor on normal development, resulted in disruption o... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320989 Sat, 04 Apr 2009 04:00:00 +0100 2320989 http://www.medworm.com/index.php?rid=2320992&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19343526%26dopt%3DAbstract In this study, a single chain variable antibody fragment (scFv) recognizing FMDV coat protein VP1 was expressed in transgenic tobacco plants. To enhance the accumulation of scFv protein, the codon-usage of a murine hybridoma-derived scFv gene was adjusted to mimic highly expressed tobacco genes and fused to an elastin-like polypeptide (ELP) tag. This scFv-ELP fusion accumulated up to 0.8% of total soluble leaf protein in transgenic tobacco. To recover scFv-ELP protein from the leaf extract, a simple and scalable purification strategy was established. Purified scFv-ELP fusion was cleaved to separate the scFv portion. Finally, it was shown that the purified scFv proteins retained their capacity to bind the FMDV in the absence or presence of ELP fusion. PMID: 19343526 [PubMed - as supplie... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320992 Fri, 03 Apr 2009 04:00:00 +0100 2320992 http://www.medworm.com/index.php?rid=2320996&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19330532%26dopt%3DAbstract This study demonstrated the complexity of factors affecting hybridization rates in Tropical America and suggested that the phenotype of F(1) hybrids facilitate their identification in the rice fields. PMID: 19330532 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2320996 Sun, 29 Mar 2009 04:00:00 +0100 2320996 http://www.medworm.com/index.php?rid=2294112&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19322672%26dopt%3DAbstract Authors: Nunes AC, Kalkmann DC, Aragão FJ Folates are essential coenzymes involved in one-carbon metabolism. Folate deficiency is associated with a higher risk of newborns with neural tube defects, spina bifida, and anencephaly, and an increased risk of cardiovascular diseases, cancer, and impaired cognitive function in adults. In plants folates are synthesized in mitochondria from pterin precursors, which are synthesized from guanosine-5'-triphosphate (GTP) in the cytosol (pterin branch), and p-aminobenzoate (PABA), derived from chorismate in plastids (PABA branch). We generated transgenic lettuce lines expressing a synthetic codon-optimized GTP-cyclohydrolase I gene (gchI) based on native Gallus gallus gene. Immunoblotting analyses confirmed the presence of the gchI in transgeni... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2294112 Thu, 26 Mar 2009 04:00:00 +0100 2294112 http://www.medworm.com/index.php?rid=2281579&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19296233%26dopt%3DAbstract In this study, we investigated differences in the protein composition of milk from control and transgenic goats expressing recombinant human butyrylcholinesterase. In Experiment 1, the milk was characterized by gel electrophoresis and liquid chromatography/mass spectrometry in order to identify protein bands that were uniquely visible in the transgenic milk and/or at differing band densities compared with controls. Differences in protein content were additionally evaluated by computer assisted band densitometry. Proteins identified in the transgenic milk only included serum proteins (i.e. complement component 3b, ceruloplasmin), a cytoskeleton protein (i.e. actin) and a stress-induced protein (94 kDA glucose-regulated protein). Proteins exhibiting evident differences in band density betwee... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2281579 Thu, 19 Mar 2009 04:00:00 +0100 2281579 http://www.medworm.com/index.php?rid=2249551&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19266305%26dopt%3DAbstract Authors: Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C The frog Xenopus is a well established vertebrate model to study the processes involved in embryogenesis and organogenesis, as it can be manipulated easily with a whole series of methods. We have expanded these approaches by establishing two transgenic Xenopus strains that allow specific interference with the activity of defined genes using a heat-shock inducible Cre recombinase that can induce upon heat-shock expression of a reporter gene in crossings to a corresponding reporter strain. We have applied this binary technique of gene interference in Xenopus development to overexpress the mutated HNF1beta transcription factor at distinct developmental stages. Induction of HNF1beta P3... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2249551 Fri, 06 Mar 2009 05:00:00 +0100 2249551 http://www.medworm.com/index.php?rid=2241574&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19263233%26dopt%3DAbstract Authors: Zhang Y, Liu H, Li B, Zhang JT, Li Y, Zhang H The aim of this research was to generate selectable marker-free transgenic tomato plants with improved tolerance to abiotic stress. An estradiol-induced site-specific DNA excision of a selectable marker gene using the Cre/loxP DNA recombination system was employed to develop transgenic tomato constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase gene from Arabidopsis thaliana. Transgenic tomato plants containing a selectable marker were also produced as controls. The expression of AtIpk2beta conferred improved resistance to drought, cold and oxidative stress in both sets of transgenic tomato plants. These results demonstrate the feasibility of using this Cre/loxP-based marker elimination strategy to generate m... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2241574 Thu, 05 Mar 2009 05:00:00 +0100 2241574 http://www.medworm.com/index.php?rid=2217177&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19241134%26dopt%3DAbstract Authors: Anterola A, Shanle E, Perroud PF, Quatrano R Taxadiene synthase gene from Taxus brevifolia was constitutively expressed in the moss Physcomitrella patens using a ubiquitin promoter to produce taxa-4(5),11(12)-diene, the precursor of the anticancer drug paclitaxel. In stable moss transformants, taxa-4(5),11(12)-diene was produced up to 0.05% fresh weight of tissue, without significantly affecting the amounts of the endogenous diterpenoids (ent-kaurene and 16-hydroxykaurane). Unlike higher plants that had been genetically modified to produce taxa-4(5),11(12)-diene, transgenic P. patens did not exhibit growth inhibition due to alteration of diterpenoid metabolic pools. Thus we propose that P. patens is a promising alternative host for the biotechnological production of paclitaxel... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2217177 Wed, 25 Feb 2009 05:00:00 +0100 2217177 http://www.medworm.com/index.php?rid=2201231&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19225900%26dopt%3DAbstract Authors: Palaudelmàs M, Peñas G, Melé E, Serra J, Salvia J, Pla M, Nadal A, Messeguer J Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate areas, we investigated the real impact of GM volunteers on the yield of 12 non-GM agricultural fields. Volunteer density varied from residual to around 10% of plants in the field and was largely reduced using certain cultural practices. Plant vigour was low, they rarely had cobs and produced pollen that cross-fertilized neighbour plants only at low-but variable-levels. In ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2201231 Thu, 19 Feb 2009 05:00:00 +0100 2201231 http://www.medworm.com/index.php?rid=2201230&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19225901%26dopt%3DAbstract Authors: Boonanuntanasarn S, Panyim S, Yoshizaki G We conducted in vitro transcription activities of the three zebrafish U6 putative promoters across species in cell extracts prepared from Nile tilapia (Oreochromis niloticus) and shrimps. The transcription efficiency of these putative U6 promoters in Nile tilapia cell extracts was similar to that of zebrafish cell extracts. In addition, all three zebrafish U6 snRNA promoters were able to express the shRNA in cell extracts prepared from two shrimp species, Penaeus monodon and Litopenaeus vannamei. However, the shRNA transcription products in shrimp cell extracts showed weaker signals. These U6 promoters could promote shRNA expression, suggesting that they have the potential for use for vector-based RNAi in Nile tilapia and shrimps. A pu... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2201230 Thu, 19 Feb 2009 05:00:00 +0100 2201230 http://www.medworm.com/index.php?rid=2191199&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19219636%26dopt%3DAbstract We describe here a novel method that solves this problem. A successive three-step gap-repair strategy was developed to replace the genomic coding sequence in mouse whey acidic protein (mWAP) gene locus with that of human lactoferrin (hLF) precisely from the start code to the end code. A 50-kb mWAP-hLF hybrid gene locus was constructed, and corresponding transgenic mice were generated. An extremely high-level expression of rhLF in the milk was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot. The expression level ranged from 16.7 to 29.8 g/l among five transgenic lines, as indicated by the ELISA assay. Importantly, the expressed rhLF maintained the same antibacterial activity as the native hLF. Our strategy can very likely also be used for the effic... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2191199 Sun, 15 Feb 2009 05:00:00 +0100 2191199 http://www.medworm.com/index.php?rid=2157098&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19184501%26dopt%3DAbstract Authors: Buscara L, Montazer-Torbati F, Chadi S, Auguste A, Laubier J, Chassot AA, Renault L, Passet B, Costa J, Pannetier M, Vilotte M, Chaboissier MC, Vilotte JL, Pailhoux E, Le Provost F RSPO1 is a newly discovered gene involved in sex differentiation. Two goat BAC clones encompassing the RSPO1 gene (gRSPO1) were injected into mouse oocytes and several transgenic lines derived. Both clones induced gRSPO1 over-expression in various tissues, including male and female gonads, with no obvious phenotype and normal sex-ratios. Introgression of the gRSPO1 transgene into a mouse RSPO1 knockout genotype resulted in the rescue of the fertility and the disappearance of the masculinized gonadic features of the females, demonstrating the functionality of the goat protein in a mouse context. On t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2157098 Fri, 30 Jan 2009 05:00:00 +0100 2157098 http://www.medworm.com/index.php?rid=2157096&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19184503%26dopt%3DAbstract Authors: Kragh PM, Nielsen AL, Li J, Du Y, Lin L, Schmidt M, Bøgh IB, Holm IE, Jakobsen JE, Johansen MG, Purup S, Bolund L, Vajta G, Jørgensen AL In an effort to develop a porcine model of Alzheimer's disease we used handmade cloning to produce seven transgenic Göttingen minipigs. The donor fibroblasts had been stably transfected with a plasmid cassette containing, as transgene, the cDNA of the neuronal variant of the human amyloid precursor protein gene with the Swedish mutation preceded by beta-globin sequences to induce splicing and a human PDGFbeta promoter fragment to drive transcription. Transgene insertion had occurred only at the GLIS3 locus where a single complete copy of the transgene was identified in intronic sequences in opposite direction. Similar and robus... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2157096 Thu, 29 Jan 2009 05:00:00 +0100 2157096 http://www.medworm.com/index.php?rid=2157095&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19184504%26dopt%3DAbstract Authors: Chakraborti D, Sarkar A, Mondal HA, Das S The phloem sap-sucking hemipteran insect, Aphis craccivora, commonly known as cowpea aphid, cause major yield loss of important food legume crop chickpea. Among different plant lectins Allium sativum leaf agglutinin (ASAL), a mannose binding lectin was found to be potent antifeedant for sap sucking insect A. craccivora. Present study describes expression of ASAL in chickpea through Agrobacterium-mediated transformation of "single cotyledon with half embryo" explant. ASAL was expressed under the control of CaMV35S promoter for constitutive expression and phloem specific rolC promoter for specifically targeting the toxin at feeding site, using pCAMBIA2301 vector containing plant selection marker nptII. Southern blot analysis demonstrated... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2157095 Thu, 29 Jan 2009 05:00:00 +0100 2157095 http://www.medworm.com/index.php?rid=2157097&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19184502%26dopt%3DAbstract Authors: Gray BN, Ahner BA, Hanson MR Homologous recombination within plastids directs plastid genome transformation for foreign gene expression and study of plastid gene function. Though transgenes are generally efficiently targeted to their desired insertion site, unintended homologous recombination events have been observed during plastid transformation. To understand the nature and abundance of these recombination events, we analyzed transplastomic tobacco lines derived from three different plastid transformation vectors utilizing two different loci for foreign gene insertion. Two unintended recombinant plastid DNA species were formed from each regulatory plastid DNA element included in the transformation vector. Some of these recombinant DNA species accumulated to as much as 10-60... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2157097 Wed, 28 Jan 2009 05:00:00 +0100 2157097 http://www.medworm.com/index.php?rid=2138281&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19169897%26dopt%3DAbstract Authors: Marusic C, Vitale A, Pedrazzini E, Donini M, Frigerio L, Bock R, Dix PJ, McCabe MS, Bellucci M, Benvenuto E The first evidence that plants represent a valid, safe and cost-effective alternative to traditional expression systems for large-scale production of antigens and antibodies was described more than 10 years ago. Since then, considerable improvements have been made to increase the yield of plant-produced proteins. These include the use of signal sequences to target proteins to different cellular compartments, plastid transformation to achieve high transgene dosage, codon usage optimization to boost gene expression, and protein fusions to improve recombinant protein stability and accumulation. Thus, several HIV/SIV antigens and neutralizing anti-HIV antibodies have recentl... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2138281 Sun, 25 Jan 2009 05:00:00 +0100 2138281 http://www.medworm.com/index.php?rid=2131666&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19165615%26dopt%3DAbstract Authors: Ocadiz-Delgado R, Marroquin-Chavira A, Hernandez-Mote R, Valencia C, Manjarrez-Zavala ME, Covarrubias L, Gariglio P Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2131666 Fri, 23 Jan 2009 05:00:00 +0100 2131666 http://www.medworm.com/index.php?rid=2129462&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19160066%26dopt%3DAbstract Authors: Woo HJ, Cho HS, Lim SH, Shin KS, Lee SM, Lee KJ, Kim DH, Cho YG Antibiotic resistance marker genes are powerful selection tools for use in plant transformation processes. However, once transformation is accomplished, the presence of these resistance genes is no longer necessary and can even be undesirable. We herein describe the successful excision of antibiotic resistance genes from transgenic plants via the use of an oxidative stress-inducible FLP gene. FLP encodes a recombinase that can eliminate FLP and hpt selection genes flanked by two FRT sites. During a transformation procedure in tobacco, transformants were obtained by selection on hygromycin media. Regenerants of the initial transformants were screened for selective marker excision in hydrogen peroxide supplemented m... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2129462 Thu, 22 Jan 2009 05:00:00 +0100 2129462 http://www.medworm.com/index.php?rid=2110912&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19145469%26dopt%3DAbstract Authors: Devos Y, Cougnon M, Vergucht S, Bulcke R, Haesaert G, Steurbaut W, Reheul D PMID: 19145469 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2110912 Thu, 15 Jan 2009 05:00:00 +0100 2110912 http://www.medworm.com/index.php?rid=2104836&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19140023%26dopt%3DAbstract Authors: De Muynck B, Navarre C, Nizet Y, Stadlmann J, Boutry M Genes encoding the heavy and light chains of LO-BM2, a therapeutic IgG antibody, were assembled in the tandem or inverted convergent orientation and expressed in Nicotiana tabacum plants and BY-2 suspension cells. The tandem construct allowed higher expression in both expression systems. A similar degradation pattern was observed for the secreted antibody recovered from the leaf intercellular fluid and BY-2 culture medium. Degradation increased with leaf age or culture time. Antibodies purified from leaf tissues and BY-2 cells were both functional. However, MS analysis of the N-glycosylation showed complex plant-type glycans to be the major type in the antibody purified from plants, whereas, oligomannosidic was the major g... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2104836 Wed, 14 Jan 2009 05:00:00 +0100 2104836 http://www.medworm.com/index.php?rid=2093048&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130282%26dopt%3DAbstract Authors: Zhang R, Rao M, Li C, Cao J, Meng Q, Zheng M, Wang M, Dai Y, Liang M, Li N Hepatitis A virus (HAV) is a wide spread pathogenic agent and is the common cause of acute Hepatitis A worldwide. Passive immunization of HAV plays an extremely important role in post-exposure prophylaxis with clinical applications often requiring large amounts of antibody. As an alternative to the in vitro production of recombinant proteins, expression of monoclonal antibodies (mAbs) in the milk of transgenic animals is currently used being associated with low production costs and high activity. In this paper, eight founder lines of transgenic mice were generated by co-microinjection of the two cassettes encoding the heavy- and light-chains of a neutralizing anti-HAV antibody, respectively. The express... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2093048 Thu, 08 Jan 2009 05:00:00 +0100 2093048 http://www.medworm.com/index.php?rid=2053398&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19093224%26dopt%3DAbstract Authors: Jerchow B In a history that goes back to 1999, the Transgenic Technology meetings started out in Sweden and over the years began to attract a growing community of technicians and researchers mainly from Europe. As the meetings started to attract an expanding worldwide audience, the community decided to found the International Society for Transgenic Technologies at the Barcelona meeting in 2005. The 2007 convention was held at Brisbane, Australia, and in 2008, the 8th Transgenic Technology meeting was held for the second time on behalf of ISTT and for the second time outside of Europe in Toronto, Canada. Due to its excellent program with over 400 participants the meeting was able to attract the highest number of delegates of all past TT meetings. With extended times for plenary... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2053398 Thu, 18 Dec 2008 05:00:00 +0100 2053398 http://www.medworm.com/index.php?rid=2053397&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19093225%26dopt%3DAbstract Authors: Yang SH, Bergo MO, Farber E, Qiao X, Fong LG, Young SG A common strategy for conditional knockout alleles is to "flox" (flank with loxP sites) a 5' exon within the target gene. Typically, the floxed exon does not contain a unit number of codons so that the Cre-mediated recombination event yields a frameshift and a null allele. Documenting recombination within the genomic DNA is often regarded as sufficient proof of a frameshift, and the analysis of transcripts is neglected. We evaluated a previously reported conditional knockout allele for the beta-subunit of protein farnesyltransferase. The recombination event in that allele-the excision of exon 3-was predicted to yield a frameshift. However, following the excision of exon 3, exon 4 was skipped by the mRNA splicing machinery,... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2053397 Thu, 18 Dec 2008 05:00:00 +0100 2053397 http://www.medworm.com/index.php?rid=2047886&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19085069%26dopt%3DAbstract Authors: Vasey DB, Lillico SG, Sang HM, King TJ, Whitelaw CB The CMV enhancer-promoter sequence is often used as a transcriptional regulatory element in vector systems. We have used this control element to drive expression of GFP in a lentivirus vector transgene in pigs and chickens. Promoted as a 'universal' enhancer/promoter element capable of transcriptional activity in a number of cells in vitro, CMV-GFP transgene expression in vivo is preferentially observed in exocrine cells. This expression profile validates the use of this transcriptional control sequence to target expression to exocrine cells in gene transfer strategies. PMID: 19085069 [PubMed - as supplied by publisher] (Source: Transgenic Research) Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2047886 Tue, 16 Dec 2008 05:00:00 +0100 2047886 http://www.medworm.com/index.php?rid=2047887&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19083116%26dopt%3DAbstract Authors: Priestley AL, Brownbridge M Traditionally, control of European corn borer (Ostrinia nubilalis) Hübner has been achieved through the use of chemical insecticides. With increasing emphasis on reducing pesticide inputs in agricultural production, alternative management technologies are now being used including transgenic silage corn modified to express Cry1Ab protein toxins derived from Bacillus thuringiensis (Bt) Berliner. The Cry1Ab toxin is expressed by all plant cells and throughout the growing season. Furthermore, the toxins are exuded from corn plant roots into the rhizosphere, raising concerns over possible side-effects on non-target beneficial organisms in the same habitat. In addition, detrivores are exposed to crop residues containing the toxin when incorporated in... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2047887 Sat, 13 Dec 2008 05:00:00 +0100 2047887 http://www.medworm.com/index.php?rid=2033889&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19067215%26dopt%3DAbstract Authors: Xu Q, Chen W, Wang Z, Zheng J, Zhang Z In order to develop a model system of infantile hemangioma, transgenic mice were developed carrying the Polyomavirus Middle T (PyMT) gene driven by the SV40 late promoter. From the 520 fertilized eggs surviving microinjection, there were 25 live births. Three of these showed the hemangioma phenotype and carried and expressed the PyMT gene; the remaining descendants were normal. The tumors showed abnormal vascular proliferation with cavernous hemangioma-like structures in the skin surface, tongue, ear mucosa and gastric mucosal tissue in the transgenic mice with hemangioma phenotype. Immunohistochemical staining for Ki-67 was negative, showing the tumors were hemangiomas rather than angiosarcomas. None of the PyMT transgenic mice survived ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2033889 Tue, 09 Dec 2008 05:00:00 +0100 2033889 http://www.medworm.com/index.php?rid=2033888&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19067216%26dopt%3DAbstract This study assesses the effect of Bt-maize on the distribution of maize viruses. Random surveys were conducted in Spain between 2001 and 2006 to evaluate the occurrence of maize viruses in Bt-maize cultivation areas and in areas where this crop had not been introduced. Maize dwarf mosaic virus (MDMV) was the predominant virus in Bt-areas, and Maize rough dwarf virus (MRDV) was the most predominant one in non-Bt-areas, with MRDV an emergent virus in both types of areas. A decline in the occurrence of MDMV and an increase in that of Sugarcane mosaic virus was observed in Bt-areas. Additionally, data obtained over 6 years in experimental fields showed non-significant differences between the infection rates exhibited by two generations of Bt varieties and the non-transformed isogenics varietie... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2033888 Tue, 09 Dec 2008 05:00:00 +0100 2033888 http://www.medworm.com/index.php?rid=2011688&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19051051%26dopt%3DAbstract Authors: McKenzie MJ, Hunter DA, Pathirana R, Watson LM, Joyce NI, Matich AJ, Rowan DD, Brummell DA Tolerance to high selenium (Se) soils in Se-hyperaccumulating plant species is correlated with the ability to biosynthesise methylselenocysteine (MeSeCys), due to the activity of selenocysteine methyltransferase (SMT). In mammals, inclusion of MeSeCys in the diet reduces the incidence of certain cancers, so increasing the range of crop plants that can produce this compound is an attractive biotechnology target. However, in the non-Se accumulator Arabidopsis, overexpression of SMT does not result in biosynthesis of MeSeCys from selenate because the rate at which selenate is reduced to selenite by ATP sulfurylase (ATPS) is low. This limitation is less problematic in other species of the Br... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2011688 Wed, 03 Dec 2008 05:00:00 +0100 2011688 http://www.medworm.com/index.php?rid=2011687&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19051052%26dopt%3DAbstract Authors: Li S, Guo Y, Shi J, Yin C, Xing F, Xu L, Zhang C, Liu T, Li Y, Li H, Du L, Chen X Live rabbits have previously been generated through nuclear transfer using adult cells as nuclear donors. We demonstrated in this study that transfected adult rabbit fibroblasts are also capable of supporting full-term development. The fibroblasts were transfected with a pEGFP-C1 plasmid using lipofectamine(trade mark) 2000, and the transgenic cells were derived from conditioned medium. The transgenic fibroblasts were cultured until confluent and then serum-starved prior to be used as nuclear donors. After nuclear transfer and activation, 22% (12/55) of the transgenic cloned embryos developed to the blastocyst stage. A total of 114 embryos at the 4- to 8-cell stage were transferred to the oviduct... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2011687 Wed, 03 Dec 2008 05:00:00 +0100 2011687 http://www.medworm.com/index.php?rid=2006590&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19043795%26dopt%3DAbstract Authors: Zhang J, Lu L, Ji L, Yang G, Zheng C TM2, a new matrix attachment region (MAR) isolated from tobacco, increases transgene expression in plants. We have carried out a more detailed analysis of the DNA elements in TM2 with the aim of improving its effect on transcription activation. Our study of the location effect of individual MARs on the expression of the adjacent 35S:gusA cassette indicated that the TM2 functions in a bidirectional manner, with the 5'-MAR being more efficient in enhancing beta-glucuronidase expression than the 3'-MAR. The influence of 5'-MAR on different linked mini-promoters in transgenic tobacco cells suggested that the role of TM2 depends on the basic expression of the transgenes. Deletion analysis of one topo II site and two unwinding sites together with... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=2006590 Sun, 30 Nov 2008 05:00:00 +0100 2006590 http://www.medworm.com/index.php?rid=1996953&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19034683%26dopt%3DAbstract Authors: Mangerich A, Scherthan H, Diefenbach J, Kloz U, van der Hoeven F, Beneke S, Bürkle A Here we report an approach to generate a knock-in mouse model using an 'ends-out' gene replacement vector to substitute the murine Parp-1 (mParp-1) coding sequence (32 kb) with its human orthologous sequence (46 kb). Unexpectedly, examination of mutant ES cell clones and mice revealed that site-specific homologous recombination was mimicked in three independently generated ES cell clones by bidirectional extension of the vector homology arms using the endogenous mParp-1-flanking sequences as templates. This was followed by adjacent integration of the targeting vector, thus leaving the endogenous mParp-1 locus functional. A related phenomenon termed 'ectopic gene targeting' has so far only... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1996953 Wed, 26 Nov 2008 05:00:00 +0100 1996953 http://www.medworm.com/index.php?rid=1991796&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19031005%26dopt%3DAbstract Authors: Echelard Y, Williams JL, Destrempes MM, Koster JA, Overton SA, Pollock DP, Rapiejko KT, Behboodi E, Masiello NC, Gavin WG, Pommer J, Van Patten SM, Faber DC, Cibelli JB, Meade HM Purified plasma derived human albumin has been available as a therapeutic product since World War II. However, cost effective recombinant production of albumin has been challenging due to the amount needed and the complex folding pattern of the protein. In an effort to provide an abundant source of recombinant albumin, a herd of transgenic cows expressing high levels of rhA in their milk was generated. Expression cassettes efficiently targeting the secretion of human albumin to the lactating mammary gland were obtained and tested in transgenic mice. A high expressing transgene was transfected in prima... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1991796 Sat, 22 Nov 2008 05:00:00 +0100 1991796 http://www.medworm.com/index.php?rid=1975949&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19015945%26dopt%3DAbstract Authors: Osorio FG, Obaya AJ, López-Otín C, Freije JM Ageing research benefits from the study of accelerated ageing syndromes such as Hutchinson-Gilford progeria syndrome (HGPS), characterized by the early appearance of symptoms normally associated with advanced age. Most HGPS cases are caused by a mutation in the gene LMNA, which leads to the synthesis of a truncated precursor of lamin A known as progerin that lacks the target sequence for the metallopotease FACE-1/ZMPSTE24 and remains constitutively farnesylated. The use of Face-1/Zmpste24-deficient mice allowed us to demonstrate that accumulation of farnesylated prelamin A causes severe abnormalities of the nuclear envelope, hyper-activation of p53 signalling, cellular senescence, stem cell dysfunction and the development ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1975949 Tue, 18 Nov 2008 05:00:00 +0100 1975949 http://www.medworm.com/index.php?rid=1955607&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19002597%26dopt%3DAbstract Authors: Hüsemann Y, Klein CA Human metastasis has been modeled mostly by xenotransplantation of cell lines in immunodeficient mice. Since this approach frequently uses cell lines derived from metastases, it ignores the significant role of cellular selection processes before and during metastatic progression and, in fact, models metastasis from metastasis and not metastasis from primary tumours. While the importance of the latter for the fate of patients is proven, the existence and clinical relevance of metastasis from metastasis is still unsettled. On the other hand, transgenic or gene knockout models of cancer offer novel experimental approaches to dissect the metastatic cascade from its very beginnings. Here, we briefly review the attempts to model metastatic progression and t... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1955607 Tue, 11 Nov 2008 05:00:00 +0100 1955607 http://www.medworm.com/index.php?rid=1902269&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18941919%26dopt%3DAbstract Authors: McPherson MA, Yang RC, Good AG, Nielson RL, Hall LM Safflower has been transformed for field scale molecular farming of high-value proteins including several pharmaceuticals. Viable safflower seed remaining in the soil seed bank after harvest could facilitate seed and pollen-mediated gene flow. Seeds may germinate in subsequent years and volunteer plants may flower and potentially outcross with commodity safflower and/or produce seed. Seeds from volunteers could become admixed with conventional crops at harvest, and/or replenish the seed bank. Seed in following crops could be transported locally and internationally and facilitate gene flow in locations where regulatory thresholds and public acceptance differ from Canada. Seed-mediated gene flow was examined in three studies. S... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1902269 Wed, 22 Oct 2008 04:00:00 +0100 1902269 http://www.medworm.com/index.php?rid=1883709&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18923916%26dopt%3DAbstract Authors: Fan B, Li N We have designed and synthesized a gene encoding a fusion protein comprising Magainin2 and a carrier protein with the aim of screening for a suitable carrier protein expressing antibacterial peptides in the mammalian expression system. The antibacterial peptide Magainin2 was used as a model. Our results on mammalian cell expression showed that there was no exceptional splicing in the transfected CHO-s cells. Analysis of the transgenic mouse model revealed that the expression level of this fusion protein in one transgenic positive mouse was up to 10 g/l, which is close to the level of beta-casein in goat milk. The bioactivity analysis showed that the digested fusion protein had antibacterial activity. These results demonstrate that the synthetic gene of the carrier ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1883709 Thu, 16 Oct 2008 04:00:00 +0100 1883709 http://www.medworm.com/index.php?rid=1880323&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18853270%26dopt%3DAbstract Authors: Wang YA, Zheng JW, Fei ZL, Jiang XQ, Wang ZG, Fei J, Zhang ZY Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vascular anomalies in vivo, as well as the study of PyMT's molecular function. A new conditional transgenic vector based on Tet-On system was constructed successfully. After the experiment in vitro, pronuclear microinjection method was used to introduce the purified transgene into the chromosomes of fertilized mice eggs, and five transgenic positive mice were obtained. The transgenic positive animals went d... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1880323 Tue, 14 Oct 2008 04:00:00 +0100 1880323 http://www.medworm.com/index.php?rid=1848203&cid=s_36128_50_f&fid=36128&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18830803%26dopt%3DAbstract Authors: Sunilkumar G, Waghela SD, Campbell LM, Rathore KS As a first step towards manufacturing functional anti-K99 single chain variable antibody fragment (scFv) in a plant system to prevent colibacillosis in neonatal calves, we investigated the feasibility of producing these antibodies in rice plants. Two scFv constructs, with or without the endoplasmic reticulum (ER) targeting KDEL sequence, were introduced into rice for either ER-retention of the recombinant antibody or its secretion. In agreement with several other published reports, extremely low-levels of scFv were produced in rice plants transformed with the construct lacking the ER-targeting sequence. Constructs containing the KDEL sequence resulted in significantly higher levels of the antibody in rice leaves. Although scFv ... Transgenic Research journals http://www.medworm.com/rss/comments.php?id=1848203 Thu, 02 Oct 2008 04:00:00 +0100 1848203