MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Ultrastructural Pathology' source. Thu, 09 Feb 2012 00:42:22 +0100 http://www.medworm.com/index.php?rid=5666264&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292732%26dopt%3DAbstract Authors: Hicks J, Mierau G, Wartchow E, Eldin K Abstract The detection of microscopic hematuria in a child's urine prompts evaluation for renal and urinary bladder causes. Microscopic hematuria identified during a routine physical examination by the pediatrician is much more common than macroscopic hematuria. Persistent microscopic hematuria is particularly worrisome and may require a percutaneous needle core kidney biopsy to determine whether the etiology is secondary to glomerular disease, tubulointerstitial disease, urinary tract infection, urinary tract structural abnormalities, medications, or toxins. This paper reviews the epidemiology, pathologic features, pathogenesis, treatment, and outcome of familial hematuria (Alport syndrome [hereditary nephritis]), thin basement membr... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666264 Wed, 08 Feb 2012 03:06:09 +0100 5666264 http://www.medworm.com/index.php?rid=5666263&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292733%26dopt%3DAbstract Authors: Pretorius E, Engelbrecht MJ, Duim W Abstract Stroke is one of the most debilitating diseases worldwide, with its occurrence increasing in Western societies. Central to the pathogenesis of thromboembolic stroke is the involvement of platelets. During thromboembolic events, nucleated cells undergo cell death, and platelets are also affected by parameters causing these incidents. Particularly, initiation of necrotic cell death at sites of vascular injury may play an important role in inducing inflammatory and repair processes. In the current research, the authors investigate whether a changed platelet ultrastructure is visible in thromboembolic stroke and whether it might be visible in platelets as apoptosis or necrosis. Therefore, in the current investigation, the authors st... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666263 Wed, 08 Feb 2012 03:06:09 +0100 5666263 http://www.medworm.com/index.php?rid=5666262&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292734%26dopt%3DAbstract This study established the epithelial nature of some tubular structures with slit-like lumina, easily misinterpreted as capillaries by light microscopy. Results also showed that the irregular spaces observed within the basaloid pattern probably result from cell discohesion. Moreover, this investigation demonstrates the epithelial nature of a subset of spindle cells within the stroma of adamantinoma and offers ultrastructural evidence for a probable mesenchymal-epithelial transformation as its histogenesis. PMID: 22292734 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666262 Wed, 08 Feb 2012 03:06:09 +0100 5666262 http://www.medworm.com/index.php?rid=5666261&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292735%26dopt%3DAbstract In conclusion, the expression of Bcl-2 and Bax in NMIBC could have a prognostic value in assessing the risk of recurrence after BCG immunotherapy. These findings require further investigations on larger cohort in order to ascertain new molecular markers of the response to BCG immunotherapy. PMID: 22292735 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666261 Wed, 08 Feb 2012 03:06:09 +0100 5666261 http://www.medworm.com/index.php?rid=5666260&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292736%26dopt%3DAbstract In this study the authors have elucidated preferential cellular vulnerability in three different brain regions. Autopsy material of the prefrontal cortex, striatum, and substantia nigra obtained from the brain tissue of alcoholic subjects was used in this study. We found that dendritic tree and astroglial damage is irreversible, while neuronal somata and most axons do not display irreversible changes. PMID: 22292736 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666260 Wed, 08 Feb 2012 03:06:09 +0100 5666260 http://www.medworm.com/index.php?rid=5666259&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292737%26dopt%3DAbstract Authors: Gu X, Liu XY, Fagan A, Gonzalez-Toledo ME, Zhao LR Abstract Pericytes, the specialized vascular smooth muscle cells (VSMCs), play an important role in supporting and maintaining the structure of capillaries. Pericytes show biochemical and physiologic features similar to VSMC, usually containing smooth muscle actin fibers and rich endoplasm reticulum. Studies have indicated that degeneration of VSMCs due to Notch3 mutations is the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). However, it remains unclear whether the Notch3 mutation also affects cerebral cortex capillary pericytes. In this ultrastructural morphologic study, the authors have observed pathological changes in the cerebral cortex capillary pericytes... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666259 Wed, 08 Feb 2012 03:06:09 +0100 5666259 http://www.medworm.com/index.php?rid=5666258&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22292738%26dopt%3DAbstract This study corroborates that PTPR has concomitant ependymal, neuroendocrine, and secretory features, and details novel ultrastructural as well as immunohistochemical features that further this argument. Discrepancies with prior descriptions of PTPR are described, as these differences may reflect phenotypic variability in this rare tumor, and the ultrastructural features that relate to the putative ependymal origin of the entity are emphasized. PMID: 22292738 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5666258 Wed, 08 Feb 2012 03:06:09 +0100 5666258 http://www.medworm.com/index.php?rid=5430857&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22085302%26dopt%3DAbstract Conclusion: With the development of epithelial ovarian tumors from benign to malignant, IEX-1 expression is decreased, concomitant with a decreased rate of cell apoptosis. Thus, IEX-1 is pro-apoptotic in the development of epithelial ovarian cancer. The pro-apoptotic activity may take part in restraining tumor growth at the early stage of ovarian epithelial cancer, whereas its decreased expression probably contributes to the abnormal survival advantage for malignant cancer. Altered IEX-1 expression can potentially be a new predictor of the malignant transformation and a prognostic indicator for cancer therapy. PMID: 22085302 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5430857 Mon, 21 Nov 2011 20:36:21 +0100 5430857 http://www.medworm.com/index.php?rid=5430856&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22085303%26dopt%3DAbstract Authors: Rodriguez-Waitkus PM, Byrd R, Hicks J Abstract Metachromatic leukodystrophy (MLD) is an autosomal recessive lysosomal storage disorder caused by a deficiency of arylsulfatase A enzyme. This deficiency leads to accumulation of sulfatides in the central nervous system and other organs, such as the gallbladder. Here the authors discuss a 9-year-old Middle Eastern patient with late-infantile-type MLD who presented with symptoms of cholecystitis. Radiographic studies revealed an enlarged gallbladder with a thickened wall and a pericholecystic fluid collection with peripheral calcifications. Gross examination of the gallbladder showed multiple small to medium-sized papillary projections involving the entire mucosal surface. Sections through the gallbladder wall revealed multiloc... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5430856 Mon, 21 Nov 2011 20:36:12 +0100 5430856 http://www.medworm.com/index.php?rid=5303741&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21981818%26dopt%3DAbstract Authors: Fidziańska A, Walczak E, Szwoch M Abstract Ultrastructural analysis was performed in cardiac ceroidlipofuscinosis to confirm the presence and the nature of storage material. Granular osmophilic deposits characteristic of GROD structures coincidented with particularly aberrant mitochondria. Remodeling of mitochondrial interior with the appearance of several form of abnormal inclusions was never observed in cardiac ceroidlipofuscinosis. The presence of dense osmophilic bodies, glycogen conglomerates, balloon-like and onion-like structures in mitochondrial interior seem to be early events of this storage process. PMID: 21981818 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5303741 Fri, 07 Oct 2011 04:00:00 +0100 5303741 http://www.medworm.com/index.php?rid=5303740&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21981819%26dopt%3DAbstract Authors: Yang B, Gao J, Rao Z, Zhang B, Ouyang W, Yang C Abstract Antisense oligonucleotide (ASODN) targeting specific gene can be capable of potently downregulating proliferation and invasion in human cancer cells. However, the underlying mechanisms are less well defined. Here the authors show that matrix metalloproteinase-7 (MMP-7) ASODN changes the ultrastructure of human A549 lung adenocarcinoma cells. Transfection of MMP-7 ASODN significantly lowered the expression of MMP-7 protein in A549 cells. Decreased microvilli, endoplasmic reticulum dilation, swelling of mitochondria, and formation of apoptotic bodies were observed by transmission electron microscope. Collectively, the findings identified the morphological mechanism that MMP-7 ASODN inhibited proliferation and invasion ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5303740 Fri, 07 Oct 2011 04:00:00 +0100 5303740 http://www.medworm.com/index.php?rid=5303739&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21981820%26dopt%3DAbstract Authors: Denardi SE, Bechara GH, de Oliveira PR, Camargo Mathias MI Abstract The present study provides ultrastructural information about the acaricidal effects of neem (Azadirachta indica A. Juss) on the ovaries of R. sanguineus engorged females. In general, the main damage caused in the oocytes was alteration in the shape of the cell and of the germinal vesicle, ring-shaped nucleolus, cytoplasmic vacuolation, and disorganization of the organelles and of the cell membranes (including the chorion), all of which indicate that these cells could be in the process of death. The results showed that azadirachtin would be an efficient acaricide agent for inhibiting and/or neutralizing the reproduction process of R. sanguineus females, impairing the reproductive ability of this species. ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5303739 Fri, 07 Oct 2011 04:00:00 +0100 5303739 http://www.medworm.com/index.php?rid=5303742&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21978187%26dopt%3DAbstract Authors: Gkogkou C, Samitas K, Foteinou M Abstract Primary pleural epithelioid mesothelioma with clear cell morphology is a particularly rare neoplasm, with only a few documented cases. Here, the authors report a case of a 76-year-old man, with a history of asbestos exposure, admitted for mild dyspnea. Radiologic examination revealed right pleural effusion and pleural thickening. Cytological examination of aspirated pleural samples was consistent with non-small cell carcinoma. Histological examination of the resected, via VATS, plural specimens was consistent with the diagnosis of clear cell epithelioid mesothelioma. The authors further analyze the main morphological and immunohistochemical features of clear cell epithelioid mesothelioma, emphasizing the algorithm for excluding oth... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5303742 Thu, 06 Oct 2011 04:00:00 +0100 5303742 http://www.medworm.com/index.php?rid=5223915&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910565%26dopt%3DAbstract Authors: Hicks J, Wartchow E, Mierau G Abstract Glycogen storage diseases (GSD) affect primarily the liver, skeletal muscle, heart, and sometimes the central nervous system and the kidneys. These unique diseases are quite varied in age of onset of symptoms, morbidity, and mortality. Glycogen storage diseases are classified according to their individual enzyme deficiency. Each of these enzymes regulates synthesis or degradation of glycogen. Interestingly, there is great phenotypic variation and variable clinical courses even when a specific enzyme is altered by mutation. Depending on the specific mutation in an enzyme, a GSD patient may have a favorable or unfavorable prognosis. With neonatal or infantile forms, some GSDs lead to death within the first year of life, whereas other gl... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5223915 Sat, 17 Sep 2011 01:16:41 +0100 5223915 http://www.medworm.com/index.php?rid=5223914&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910566%26dopt%3DAbstract Conclusions: To the authors' knowledge, this is the first study indicating CK10 expression in the human testis during normal and abnormal spermatogenesis. The varied expression of CK10 in testes showing abnormal spermatogenesis suggests its possible involvement in this process. PMID: 21910566 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5223914 Sat, 17 Sep 2011 01:16:32 +0100 5223914 http://www.medworm.com/index.php?rid=5223913&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910567%26dopt%3DAbstract Authors: Sachanonta N, Chotivanich K, Chaisri U, Turner GD, Ferguson DJ, Day NP, Pongponratn E Abstract Ultrastructural changes to P. falciparum-infected red blood cells were examined in vitro after treatment with antimalarial drugs. Artesunate had the most rapid parasitocidal effect. All three drugs caused structural changes within the parasite, including dilatation of the parasitophorus vacuole membrane, depletion of ribosomes, mitochondrial swelling, and decreased formation of hemozoin crystals. The structure of surface knobs and Maurer's clefts were similar to controls but reduced in number. Only depletion of free ribosomes correlated with antimalarial drug exposure. Drug treatment decreased movement of hemozoin granules within parasites on real-time microscopy, before recogniz... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5223913 Sat, 17 Sep 2011 01:16:23 +0100 5223913 http://www.medworm.com/index.php?rid=5223912&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910568%26dopt%3DAbstract Authors: Baltatzis GE, Voloudakis GE, Arnogiannakis N, Misitzis J, Voloudakis-Baltatzis IE Abstract The tubular carcinoma of the breast is an uncommon histological subtype of invasive breast cancer, which is generally associated with an excellent prognosis. Previous studies have demonstrated that this well differentiated variant is linked with a low incidence of lymph node involvement, a low rate of local recurrence and a high overall survival rate when compared to standard invasive ductal carcinoma. Due to its favorable prognosis, some studies have proposed that a diagnosis of tubular carcinoma might warrant less aggressive surgical or adjuvant treatment. Histologically, tubular carcinoma may mimic sclerosing adenoma or bluntduct adenosis. Its ductal nature appears well confirmed ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5223912 Sat, 17 Sep 2011 01:16:13 +0100 5223912 http://www.medworm.com/index.php?rid=5169743&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21867406%26dopt%3DAbstract Authors: Shiizaki K, Hatamura I, Mato M, Nakazawa E, Saji F, Onishi A, Ogura M, Watanabe Y, Kusano E Abstract The detailed ultrastructural changes of uremia-induced hyperplastic parathyroid gland and the effects of current medical treatments for secondary hyperparathyroidism were investigated. Marked enlargement of parathyroid cell with accumulation of mitochondria and lipids and a significant increase in the thickness of the pericapillary area with increased fibrosis and appearance of fibroblast like cells were noted in the hyperplastic gland caused by uremia and phosphate retention. These ultrastructural changes and biochemical findings indicating hyperparathyroidism were significantly suppressed by all of the treatment using phosphate restriction, calcitriol, and cinacalcet. The... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5169743 Wed, 24 Aug 2011 23:00:00 +0100 5169743 http://www.medworm.com/index.php?rid=5169742&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21867407%26dopt%3DAbstract Authors: Khubchandani SR, Kulkarni S, Teckchandani S, Chitale A Abstract The incidence of Helicobacter pylori gastritis is high in India and the number of individuals with vitamin B(12) deficiency is also large. An association has been found between these two factors. It is necessary to determine whether H. pylori infection may be a factor in the causation of B(12) deficiency and whether it is associated with any morphological changes on ultrastructural examination. A cohort-based study has been performed, which includes 505 young asymptomatic males. These cases have been investigated for presence of H. pylori and macrocytosis. The study confirms an association between H. pylori infection and B(12) deficiency. It is recommended that H. pylori infection be looked for in subjects hav... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5169742 Wed, 24 Aug 2011 23:00:00 +0100 5169742 http://www.medworm.com/index.php?rid=5146470&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21843055%26dopt%3DAbstract Authors: Lloreta J, Juanpere N, Riverola A, Dallari D, Cañas MA, Pijuan L, Baró T, García P, Serrano S Abstract A case of cardiac myxoma with glandular differentiation is reported. The patient did not have elements of the Carney triad or syndrome. The tumor was mainly composed of characteristic stellate cells in a focally collagenized, myxoid stroma, along with aggregates of glandular-forming epithelial cells, with mucin-containing intra- and intercellular lumina. Ultrastructurally, these gland spaces displayed short, straight microvilli and junctional complexes. The epithelial cells were positive for cytokeratin 7 and negative for cytokeratin 20. Calretinin was positive in the stellate cells and negative in the epithelial component. The potential origin from pluripotent mesench... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5146470 Sun, 14 Aug 2011 23:00:00 +0100 5146470 http://www.medworm.com/index.php?rid=5146469&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21843056%26dopt%3DAbstract Authors: Perrotta I Abstract Healthy human arteries are composed of three layers: the intima, the media, and the adventitia. Endothelial cells, which form the tunica intima, provide the physical interface between blood and surrounding tissue, regulate nutrient and blood component traffic, and participate in many physiologic events, such as hemostasis, inflammation, and angiogenesis. Within the tunica media, smooth muscle cells and extracellular matrix proteins, such as elastin, collagen, and proteoglycans, are quantitatively the largest components of the aortic vascular wall. The structural changes with atherosclerosis are currently considered degenerative phenomena, which primarily involve a sequence of reactions within the intima and include monocyte recruitment and macrophage fo... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5146469 Sun, 14 Aug 2011 23:00:00 +0100 5146469 http://www.medworm.com/index.php?rid=5146468&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21843057%26dopt%3DAbstract Conclusions: The umbilical skin of females has abundant sensory innervation similar to that of the glans penis. PMID: 21843057 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5146468 Sun, 14 Aug 2011 23:00:00 +0100 5146468 http://www.medworm.com/index.php?rid=5031841&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21736426%26dopt%3DAbstract Authors: Juanpere-Rodero N, Martin-Ezquerra G, Fernandez-Casado A, Magan-Perea L, Garcia-Alguacil MA, Barranco-Sanz C, Serrano-Figueras S, Pujol-Vallverdu RM, Lloreta-Trull J There are limited reports on the ultrastructure of syphilis skin lesions. The aim of this study has been to perform an electron microscopic investigation of the morphology and the tissue distribution of treponemes in primary and secondary cutaneous lesions. Three cases of primary syphilitic chancre and one case of secondary syphilis were included. Prominent epidermal abnormalities in the primary chancre and a perivascular inflammatory infiltrate in the secondary lesion were found by light microscopy. Ultrastructurally, spirochetes were located mainly in the blood vessel walls and dermal tissue of the chancre lesio... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=5031841 Wed, 06 Jul 2011 23:00:00 +0100 5031841 http://www.medworm.com/index.php?rid=4934904&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657816%26dopt%3DAbstract Authors: Berner A, Sauer T Fine-needle aspiration cytology (FNAC) is an established, highly accurate, and cost-effective method for diagnosing lesions in different organs, including the breast. The method is minimally invasive without unwanted side effects. FNAC forms part of the triple assessment of breast lesions. Despite some shortcomings of the reporting categories, FNAC as part of the triple assessment has proved its value in describing the findings most accurately. The diagnostic impact depends on experience of the operator, quality of preparation, and diagnostic skills of the cytopathologist. The highest accuracy is achieved at centers with a multidisciplinary approach. FNAC is often palpation guided from palpable breast masses, whereas ultrasonography guidance is more widely us... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934904 Wed, 08 Jun 2011 23:00:00 +0100 4934904 http://www.medworm.com/index.php?rid=4934903&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657817%26dopt%3DAbstract Authors: Ru YX, Li YC, Zhao Y, Zhao SX, Yang JP, Zhang HM, Pang TX To further understand the pathological characteristics of multiple organ involvement of the 2009 pandemic influenza A/H1N1 infection, tissues of bronchial mucosa, lung, myocardium, gastrocnemius, and liver from 3 patients with fatal A/H1N1 infections were investigated by light microscopy and transmission electron microscopy. In all 3 patients, bronchial mucosa showed necrotizing bronchiolitis, epithelial necrosis and desquamation, and squamous metaplasia, while lung consolidation or fibrosis was identified. Myocardium and gastrocnemius exhibited focal necrosis and fibrosis, surrounded by muscle cells showing features of cell damage. In liver, there was widespread fatty degeneration and necrosis, most often around the ce... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934903 Wed, 08 Jun 2011 23:00:00 +0100 4934903 http://www.medworm.com/index.php?rid=4934902&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657818%26dopt%3DAbstract Authors: Darouich S, Goucha R, Jaafoura MH, Zekri S, Maiz HB, Kheder A Obesity-related glomerulopathy (ORG) is a secondary form of focal segmental glomerulosclerosis (FSGS) occurring in obese patients with a body-mass index higher than 30 kg/m(2). It is typically manifested by nephrotic-range proteinuria without full nephrotic syndrome, and progressive renal insufficiency. Characteristic morphologic features include the consistent presence of glomerulomegaly, predominance of perihilar variant of FSGS, and the relatively mild fusion of visceral epithelial cell foot processes. The concept of podocyte depletion as a driver of the glomerular scarring in obesity-associated FSGS is well documented. The underlying mechanisms are likely to be related in part to the oxidative stress and the i... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934902 Wed, 08 Jun 2011 23:00:00 +0100 4934902 http://www.medworm.com/index.php?rid=4934901&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657819%26dopt%3DAbstract Authors: Perrotta I, Perrotta E, Guido C, Tripepi S, Donato G, Aquila S, Rosato EG The term aneurysm is used to indicate a permanent and irreversible localized vascular dilatation that involves all three layers of the blood vessel wall. It may develop in any part of the circulatory system, including veins, and its history, presentation, and management differ depending on the location. A venous aneurysm is defined as a solitary area of fusiform or saccular dilatation occurring in the course of a major vein or connected by a single channel to a major venous structure. The lower extremities are the most frequently affected, with the popliteal vein being the most common site. Although different theories have been advanced, the etiology of the disease remains uncertain. Mechanical stress an... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934901 Wed, 08 Jun 2011 23:00:00 +0100 4934901 http://www.medworm.com/index.php?rid=4934900&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657820%26dopt%3DAbstract This report ultrastructurally illustrates the giant lysosome-related organelles in the various cellular components of a renal biopsy from a 10 year-old female affected by the Chédiak-Higashi syndrome. Albeit similar observations have been made and reported in animal models of the syndrome, to the author's knowledge, this is the first illustration of the changes as demonstrated in a human renal biopsy. PMID: 21657820 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934900 Wed, 08 Jun 2011 23:00:00 +0100 4934900 http://www.medworm.com/index.php?rid=4934899&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21657821%26dopt%3DAbstract Authors: Caruso RA, Parisi A, Quattrocchi E, Scardigno M, Branca G, Parisi C, Lucianò R, Paparo D, Fedele F A histological variant of gastric adenocarcinoma, characterized by an intense tumor-associated tissue eosinophilia (TATE), has been occasionally reported in the literature. The purpose of this ultrastructural study was to determine the interactions between frequently occurring eosinophils and tumor cells in gastric carcinoma characterized by TATE. Fresh tumor tissue of 92 gastric carcinomas was processed for both light and electron microscopic examination. Intense TATE was found in 7 out of 92 (7.6%) gastric carcinomas (6 of intestinal-type and 1 of diffuse-type). Electron microscopy, selectively performed in 7 cases with intense TATE, revealed eosinophils, singly or in groups, ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4934899 Wed, 08 Jun 2011 23:00:00 +0100 4934899 http://www.medworm.com/index.php?rid=4840611&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21568621%26dopt%3DAbstract This study investigates the use of a grid, placed onto a micrograph, to quantify changes in morphology. The fibrin fiber micrographs of a healthy donor were compared to a database of donors and were shown to be a true representative of a typical healthy individual. Eighteen micrographs of this single donor were taken at 40,000× machine magnification, and a grid was placed over the micrographs. The grid dimensions were calculated by using the scale bar inserted onto the micrograph. Each grid block was equal to 0.5 by 0.5 µm for a total grid area of 28 µm(2). A percentage changed fibrin fiber morphology was then calculated for each 28 µm(2) of fibrin clot produced in the laboratory. It is concluded that this effortless and simple grid technique to quantify changes in ultrastructure... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4840611 Thu, 12 May 2011 23:00:00 +0100 4840611 http://www.medworm.com/index.php?rid=4840610&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21568622%26dopt%3DAbstract Authors: Chen W, Yang S, Shi H, Guan W, Dong Y, Wang Y, Wang L Nephrosialidosis is a rare subgroup of sialidosis characterized by a progressive and fatal course of nephropathy. The authors report a 2-year-old boy who had suffered from steroid-resistant nephrotic syndrome. Renal biopsy showed diffuse and severe vacuolization of glomerular and tubular epithelial cells. The vacuoles were ultrastructurally membrane bound, most of which were not empty but contained electron-dense material lining the inner surface of the membrane. The pathologic changes were consistent with neuraminidase-deficient disorders, which was later confirmed by biochemical analysis. Although rare, nephrosialidosis should be considered in children with steroid-resistant nephrotic syndrome if renal biopsies exhibit se... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4840610 Thu, 12 May 2011 23:00:00 +0100 4840610 http://www.medworm.com/index.php?rid=4618046&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21405949%26dopt%3DAbstract Authors: Shrivastava M, Vivekanandhan S Platelets are evinced as a systemic tool in a variety of disorders, including neurodegenerative diseases. Evidence suggests that variations in the ultrastructure and morphology of platelets and related organelles are involved in the pathophysiology of diabetes, cancer, HIV/AIDS, cardiovascular and neurological diseases. Due to structural alterations of platelets in many diseases, it is informative to discuss the ultrastructural and morphological discrepancies of platelets in contemporary medical research. The present review reveals the usefulness of ultrastructural study in better understanding of the disease patterns and may help to improve the treatment regimes. PMID: 21405949 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4618046 Tue, 22 Mar 2011 19:45:19 +0100 4618046 http://www.medworm.com/index.php?rid=4618045&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21405950%26dopt%3DAbstract Authors: Zhao JF, Chen JY, Mi L, Wang PN, Peng Q The arginine-rich cationic Tat peptides have been reported to enhance the intracellular delivery of macromolecules, including DNA, RNA, and proteins. In this work an arginine cationic peptide derived from the HIV-1 Tat protein was conjugated with noncovalent bonds to sulfonated aluminum phthalocyanine (AlPcS, a photosensitizer for the light-activated photodynamic cancer therapy), doxorubicin (DOX, a chemotherapeutic agent), or quantum dots (QDs, often used as carriers for the delivery of anticancer drugs). The fluorescence of intracellular conjugates of AlPcS-Tat, DOX-Tat, and QDs-Tat was studied by means of confocal laser scanning microscopy in the human nasopharyngeal carcinoma KB cells and cervical carcinoma Hela cells in vitro. The T... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4618045 Tue, 22 Mar 2011 19:45:14 +0100 4618045 http://www.medworm.com/index.php?rid=4618044&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21405951%26dopt%3DAbstract Authors: PMID: 21405951 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4618044 Tue, 22 Mar 2011 19:45:08 +0100 4618044 http://www.medworm.com/index.php?rid=4502092&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299344%26dopt%3DAbstract Authors: Shrivastava M, Das TK, Behari M, Pati U, Vivekanandhan S Platelets are characterized as a systemic tool to elucidate mitochondria-allied perturbance in neurological diseases. The authors studied ultrastructural changes in platelets and platelet mitochondria using a case-control approach in amyotrophic lateral sclerosis (ALS). Subjects were sporadic ALS cases (n?=?22) and age- and sex-matched controls (n?=?16). Phlebotomy was performed, platelet concentrates (PCs) were prepared, and mitochondria were extracted. PCs and mitochondria were processed for ultrastructure study using transmission electron microscopy. Image analysis was done using Image-J. Transmission electron microscopy demonstrated both qualitative and quantitative variations in ALS platelets and platelet mitochondr... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502092 Tue, 22 Feb 2011 04:00:57 +0100 4502092 http://www.medworm.com/index.php?rid=4502091&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299345%26dopt%3DAbstract Authors: Tyritzis SI, Zachariades M, Evangelou K, Gorgoulis VG, Kyroudi-Voulgari A, Pavlakis K, Troupis TG, Constantinides CA Ischemia?reperfusion injury can be detrimental to the solitary kidney, especially when it is accompanied by ablation. To the authors? knowledge, the effects of partial nephrectomy with prolonged application of ischemia have never been described at the ultrastructural level. Therefore, the authors used an animal model and focused on putative structural effects in the glomerular basement membrane and the podocytes. They demonstrate the advantageous role of cold ischemia, even in up to 120?min. In contrast, more than 60?min of warm ischemia leads to catastrophic lesions in all the cellular structures, as is reflected by mortality due to acute renal failure. PMI... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502091 Tue, 22 Feb 2011 04:00:53 +0100 4502091 http://www.medworm.com/index.php?rid=4502090&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299346%26dopt%3DAbstract Authors: Caruso R, Fedele F, Lucian R, Branca G, Parisi C, Paparo D, Parisi A Mitotic catastrophe is a common phenomenon occurring in tumor cells with impaired p53 function exposed to various cytotoxic and genotoxic agents. The defective p53 checkpoint causes improper segregation of chromosomes, resulting in aberrant mitosis, multiple micronuclei, multinucleate giant cells, and eventual necrosis-like death and centrosome aberration. Although various descriptions explaining mitotic catastrophe exist, there is still no generally accepted definition of this phenomenon. However, the syndrome of mitotic catastrophe may be a unifying morphological concept of particular interest to cancer research, as it integrally links cell death to checkpoints of the cell cycle. Morphological findings comp... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502090 Tue, 22 Feb 2011 04:00:49 +0100 4502090 http://www.medworm.com/index.php?rid=4502089&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299347%26dopt%3DAbstract Authors: L X, Xu K, L H, Yin Y, Ma C, Liu Y, Li H, Suo Z CD44(+)/CD24(?) cells have been associated with breast cancer stem/progenitor cell features. However, the status of this phenotype cells in normal, benign and malignant breast tissues has not been studied, and the clinical correlation of this subpopulation in breast cancer is not fully understood. The present study sought to identify these cells in a series of normal, benign, and malignant breast tissues and explore their correlation to the molecular subtypes of breast carcinoma and conventional pathological features. Double-staining immunohistochemistry (DIHC) of CD44 and CD24 was performed on 30 normal breast tissues, 30 breast fibroadenomas (FA), 60 breast invasive ductal carcinomas (IDC), and 3 breast cancer cell lines (MCF-7... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502089 Tue, 22 Feb 2011 04:00:45 +0100 4502089 http://www.medworm.com/index.php?rid=4502088&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299348%26dopt%3DAbstract Conclusions: This study provides the first indication about the clinicopathologic features of RCCs in Upper Egypt. The variable expression of these molecules in the different variants of RCC suggests that several oncogenic pathways are operational in their development. PMID: 21299348 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502088 Tue, 22 Feb 2011 04:00:40 +0100 4502088 http://www.medworm.com/index.php?rid=4502087&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299349%26dopt%3DAbstract Authors: Khubchandani SR, Vohra P, Chitale AR, Sidana P Microvillous inclusion disease is a rare disorder of infancy associated with protracted diarrhea. This malady reveals distinct ultrastructural changes. The surface enterocytes of the duodenum show vesicles lined with microvilli and the surface microvilli are poorly formed. The authors present one case of microvillous inclusion disease with a review of the literature. PMID: 21299349 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502087 Tue, 22 Feb 2011 04:00:36 +0100 4502087 http://www.medworm.com/index.php?rid=4502086&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299350%26dopt%3DAbstract Authors: Kochar O, Wick MR, Kerr SE, Oglesbee D, Cathro HP Fabry disease is an X-linked recessive lysosomal storage disease caused by a deficiency of ?-galactosidase A, with characteristic ultrastructural cytoplasmic myelin-like inclusions. Renal lesions are seen in male and variably in heterozygous female patients. One previous report has described Fabry disease involving a renal allograft from a deceased female donor with no history of Fabry disease. The authors describe another case, in which suspicion for Fabry disease was raised ultrastructurally. This serves as a reminder that proteinuria after renal transplantation may be due to donor-derived disease. Fabry disease is probably an underrecognized cause of graft dysfunction. This case provides further justification for ultrastruct... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502086 Tue, 22 Feb 2011 04:00:33 +0100 4502086 http://www.medworm.com/index.php?rid=4502085&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299351%26dopt%3DAbstract Authors: Greenwood MD, Ressler MJ, Audette JL, Laturnus DI, Chhoun R, Teiken JM, Carlson EC The diabetic-prone BioBreeding Wistar (BB/DP) rat is an autoimmune model of insulin-dependent diabetes mellitus. Approximately 90% of the animals (BB/DPh) are hyperglycemic by 90?120 days of age, while the remaining ~10% (BB/DPn) and diabetes-resistant rats (BB/DR) are normoglycemic for life. The transmission electron microscope data from this study demonstrate expected significant age- and diabetes-related increases in retinal capillary basement membrane (RCBM) widths in (BB/DPh) rats relative to BB/DR animals. However, the data show, for the first time, an unexpected significant RCBM thickening in (BB/DPn) rats compared to BB/DR animals at 6 months and 1 year post-onset of hyperglycemia. P... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502085 Tue, 22 Feb 2011 04:00:29 +0100 4502085 http://www.medworm.com/index.php?rid=4502084&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323418%26dopt%3DAbstract Authors: Deen S, Ashford K, Harwood A, Gray T Actinomyces israelii are gram-positive filamentous bacteria forming yellow sulfur granules. They are the most well known complication of intrauterine contraceptive devices (IUCD). Healthcare staff dealing with reporting cervical smears should be aware of pseudoactinomyces entity in a cervical smears and biopsies as it may raise a false alarm to the clinician and may lead to unnecessary removal of IUCD and/or medical treatment. PMID: 21323418 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502084 Wed, 16 Feb 2011 00:00:00 +0100 4502084 http://www.medworm.com/index.php?rid=4502083&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323419%26dopt%3DAbstract Authors: Lucchese A, Petruzzi M, Scivetti M, Pilolli GP, Di Bisceglie MB, Crincoli V, Lajolo C, Giuliani M, Calabro M, Guida A, Laino L, Serpico R, Favia G The so-called calcifying odontogenic cyst (COC) represents a heterogeneous group of lesions that exhibit a variety of clinico-pathologic features. It is an uncommon lesion and represents less than 2% of all odontogenic cysts and tumors. Recently, these lesions have been reclassified as calcifying cystic odontogenic tumors (CCOT), according to the new World Health Organization (WHO) classification. CCOT are frequently found in association with, or containing areas histologically identical to, various types of odontogenic tumors, such as complex/compound odontomas. This work analyzed clinical and histological data deriving from 13 pat... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502083 Wed, 16 Feb 2011 00:00:00 +0100 4502083 http://www.medworm.com/index.php?rid=4502082&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323420%26dopt%3DAbstract Authors: Grzanka D, Marszałek A, Izdebska M, Gackowska L, Andrzej Szczepanski M, Grzanka A The effect of doxorubicin on the expression of cofilin and actin in CHO AA8 cells was estimated by fluorescence and electron microscopy. The presence of cofilin and actin was observed particularly in the nuclei of cells by different modes after treatment by doxorubicin. Cells undergoing mitotic catastrophe expressed some entirely characteristic features together with overlapping elements of other types of cell death. Additionally, the authors suggest that, as defined here, reorganization of F-actin might be involved in all cell death processes. Changes in the nuclear expression of cofilin are related to F-actin cytoplasm-nuclear translocation and its intranuclear dynamic reorganization. PMID... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502082 Wed, 16 Feb 2011 00:00:00 +0100 4502082 http://www.medworm.com/index.php?rid=4502081&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323421%26dopt%3DAbstract Authors: Adly MA, Hussein MR CD1d is a member of CD1 family of transmembrane glycoproteins, which represent antigen-presenting molecules. Immunofluorescent staining methods were utilized to examine expression pattern of CD1d in human testicular specimens. In testis showing normal spermatogenesis, a strong CD1d cytoplasmic expression was seen the Sertoli cells, spermatogonia, and Leydig cells. A moderate expression was observed in the spermatocytes. In testes showing maturation arrest, CD1d expression was strong in the Sertoli cells and weak in spermatogonia and spermatocytes compared to testis with normal spermatogenesis. In Sertoli cell only syndrome, CD1d expression was strong in the Sertoli and Leydig cells. This preliminary study displayed testicular infertility-related changes in ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502081 Wed, 16 Feb 2011 00:00:00 +0100 4502081 http://www.medworm.com/index.php?rid=4502080&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323422%26dopt%3DAbstract Authors: Strøm EH, Sund S, Reier-Nilsen M, Dørje C, Leren TP Familial lecithin:cholesterol acyltransferase (LCAT) deficiency is a rare metabolic disease with lipid deposition in several organs. The authors report a man with hypertension and proteinuria. Renal biopsy revealed glomerular changes, including peculiar thrombus-like deposits, consistent with LCAT deficiency. He was found to be compound heterozygous for two mutations of the LCAT gene. He received a kidney graft from his father. The authors also describe LCAT deficiency-related lesions in the explanted native kidneys and in biopsies at 2 days, 6 weeks, and 1 year after transplantation. The morphology of this disease is characteristic, and the diagnosis should be suspected from the ultrastructural findings. PMID: 21323422... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4502080 Wed, 16 Feb 2011 00:00:00 +0100 4502080 http://www.medworm.com/index.php?rid=4445499&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21265632%26dopt%3DAbstract This study was designed to investigate the protective effects of caffeic acid phenethyl ester on carbon tetrachloride-induced liver damage in rats. Twenty-four male Wistar rats were divided in three groups. Group I was used as control. Rats in group II were injected with carbon tetrachloride every other day for 1 month, whereas rats in group III were injected with carbon tetrachloride and caffeic acid phenethyl ester every other day for 1 month. At the end of the experiment, all animals were killed by decapitation and their livers were removed. Liver tissues were processed for electron microscopy. Histopathologically, hepatocytes of rats treated with carbon tetrachloride had damage in the cytoplasmic organelles and nuclei membranes as well as an excessive lipid accumulation in the hepatocy... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4445499 Tue, 01 Feb 2011 00:00:00 +0100 4445499 http://www.medworm.com/index.php?rid=4445498&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21265633%26dopt%3DAbstract Authors: Zanna P, Lucchese A, Sevilla MC, Maida I, Fiore MG, Rossi R, Piscitelli D, Favia G, Guida G Melanomas of the oral cavity are extremely rare. Their rarity and their independence on exposure to UV radiation make them particularly interesting. The authors analyzed an oral multiphasic melanoma composed by a nodular nonpigmented ulcerated central region, a nodular ulcerated pigmented area, a pigmented nonulcerated region, and an area similar to a dysplastic nevus. They determined the expression of some genes involved in the differentiation and cellular transformation in morphologically different regions of melanoma. All these areas were also analyzed by electron microscopy. The various regions composing the melanoma expressed genes involved in melanogenesis and melanoma progression... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4445498 Tue, 01 Feb 2011 00:00:00 +0100 4445498 http://www.medworm.com/index.php?rid=4445497&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21265634%26dopt%3DAbstract Authors: Darouich S, Goucha R, Jaafoura MH, Zekri S Membranoproliferative glomerulonephritis with isolated C3 deposits (MPGNC3) is an uncommon condition characterized by overt glomerular C3 deposits in the absence of immunoglobulins and intramembranous dense deposits. Here the authors describe the clinical and morphological features of primary MPGNC3 in a 13-year-old boy and critically review the previously published cases. The patient presented with nephrotic syndrome and microscopic hematuria. Blood tests revealed very low circulating C3 levels. The renal biopsy exhibited subendothelial, subepithelial, and mesangial deposits, with C3 but not immunoglobulins seen on immunofluorescence. This case and the review of the literature indicate that the serum complement profile with decreased... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4445497 Tue, 01 Feb 2011 00:00:00 +0100 4445497 http://www.medworm.com/index.php?rid=4382979&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21214404%26dopt%3DAbstract Conclusion: Electron microscopy has a high rate of detecting CN. A combination of histochemical staining and electron microscopy can make an accurate diagnosis of PC. PMID: 21214404 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4382979 Sat, 08 Jan 2011 00:00:00 +0100 4382979 http://www.medworm.com/index.php?rid=4382978&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21214405%26dopt%3DAbstract Authors: Liberski PP, Sikorska B, Gibson P, Brown P The authors report here robust autophagy observed by electron microscopy in both the Echigo-1 strain of Creutzfeldt-Jakob disease in hamsters and the Fujisaki strain of Gerstmann-Sträussler-Scheinker disease in mice. In both models, autophagic vacuoles were observed in several cellular compartments. In neuronal cell bodies, autophagic vacuoles of different size were seen. The cytoplasm of some neurons also contained semicircular cisterns equivalent to an early autophasophore. The major target of autophagy was dystrophic neurites, i.e., enlarged neuritic processes-mostly dendrites but also axonal terminals and preterminals. They contained numerous double- or multiple-membrane-bound autophagosomes or autophagolysosomes and large multiv... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4382978 Sat, 08 Jan 2011 00:00:00 +0100 4382978 http://www.medworm.com/index.php?rid=4162797&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070162%26dopt%3DAbstract Authors: Bárcena C, Martínez MA, Ortega MP, Muñoz HG, Sárraga GU Renal oncocytoma and chromophobe renal cell carcinoma (CRCC) are closely related tumors. They are considered the extremes of a spectrum with several variants. Ultrastructural examination of the mitochondria is a helpful procedure in the diagnosis of these neoplasms. Renal oncocytomas show mitochondria with piled lamellar cristae, and CRCC exhibited mitochondria with tubulovesicular cristae. In a series of 23 histologically diagnosed renal oncocytomas examined by electron microscopy, the authors found 5 tumors exhibiting more cells with mitochondria showing tubulovesicular cristae. The authors believe these 5 cases present a submicroscopic appearance intermediate between renal oncocytoma and CRCC, although with benign ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162797 Sat, 13 Nov 2010 18:55:58 +0100 4162797 http://www.medworm.com/index.php?rid=4162796&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070163%26dopt%3DAbstract Conclusion: Besides electron microscopy identification of inclusions, the light microscopic morphological features together with the biomarker studies highlighting the apical cytoplasmic blush are quite unique and diagnostic of MVID. Furthermore, it is the opinion of the authors that a diagnosis of MVID can be made without electron microscopy. PMID: 21070163 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162796 Sat, 13 Nov 2010 18:55:51 +0100 4162796 http://www.medworm.com/index.php?rid=4162795&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070164%26dopt%3DAbstract Authors: Alroy J, Pfannl R, Slavov D, Taylor MR Danon disease is a rare lysosomal disorder. It is due to deficiency of lysosomal-associated protein-2. In human LAMP-2 gene is located at chromosome region Xq24. Danon disease is characterized by hypertrophic cardiomyopathy, skeletal myopathy, mental retardation and retinopathy. To date, the morphological characterization of Danon disease has been limited to endomyocardial and skeletal muscle biopsies. In the current study we demonstrated that electron microscopy of a more accessible tissue, skin biopsies, is a useful method in the diagnosis of Danon disease. PMID: 21070164 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162795 Sat, 13 Nov 2010 18:55:44 +0100 4162795 http://www.medworm.com/index.php?rid=4162794&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070165%26dopt%3DAbstract Authors: Seretis E, Konstantinidou A, Arnogiannakis N, Xinopoulos D, Voloudakis-Baltatzis IE A primary mucinous colorectal adenocarcinoma tissue with signet-ring cells, as revealed after histological evaluation, was examined ultrastructurally. The authors also analyzed the immunohistochemical data of the tissue for serotonin, vasoactive intestinal polypeptide (VIP), bombesin, somatostatin, and glucagon, using the peroxidase anti-peroxidase (PAP) method and the immunogold labeling method for light and electron microscope, respectively. Electron microscopically mucinous adenocarcinoma was characterized by the formation of small lumen. Adenocarcinoma cells were full of mucous granules of varying electron density, providing a good environment for the tumor cells to grow. They also exhibite... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162794 Sat, 13 Nov 2010 18:55:36 +0100 4162794 http://www.medworm.com/index.php?rid=4162793&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070166%26dopt%3DAbstract Authors: Pasquinelli G, Papadopulos F, Nigro M Nanobacteria are controversial infectious agents with nanometric size, the capacity to nucleate hydroxyapatite and grow in culture, and present in human diseases associated with calcification and psammoma bodies. The authors report a case of pathological placental calcifications associated with nanobacteria. Electron microscopy and electron energy loss spectroscopy imaging were used to recognize 160-nm-sized calcium-free bodies mainly presenting as extracellular fibrillary tangles and 500-nm-sized calcified bodies; they encrusted the syncito-trophoblast basal membrane and aggregated into miniaturized psammoma bodies. Nanobacteria may be composed of a prionoid protein with self-assembling and self-propagating abilities whose growth is assoc... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162793 Sat, 13 Nov 2010 18:55:29 +0100 4162793 http://www.medworm.com/index.php?rid=4162792&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070167%26dopt%3DAbstract Authors: Liberski PP, Sikorska B, Hauw JJ, Kopp N, Streichenberger N, Giraud P, Boellaard J, Budka H, Kovacs GG, Ironside J, Brown P The authors report on a large series of human prion diseases to establish ultrastructural characteristics that may be useful for their diagnosis. For Creutzfeldt-Jakob disease (CJD and its variant, vCJD) and fatal familial insomnia (FFI) only vacuolation (spongiform change) and the presence of tubulovesicular structures are consistent findings. Other changes, such as the presence of myelinated vacuoles, branching cisternae, neuroaxonal dystrophy, and autophagic vacuoles, were present in different proportions in either CJD or FFI, but they are nonspecific ultrastructural findings that can also occur in other neurodegenerative conditions. The hallmark of Ge... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162792 Sat, 13 Nov 2010 18:55:22 +0100 4162792 http://www.medworm.com/index.php?rid=4162791&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070168%26dopt%3DAbstract Authors: Talmon GA, Wisecarver JL Ischemia-reperfusion injury (IR) of solid organ allografts is a consequence of ischemia resulting from disruption of blood flow during organ harvest and transportation. Histologically, this manifests as variable necrosis in a pattern similar to that seen in systemic hypoperfusion. Calcification of hepatocytes has been rarely observed in ischemic injury due to systemic shock and in two cases of severe IR, both of which were associated with graft loss and death. The authors present another case of dystrophic calcification within hepatocytes occurring in a liver allograft affected by severe IR. Biochemical stains revealed that the mineralized material was calcium phosphate (likely hydroxyapatite). By electron microscopy, the hepatocyte cytoplasm was fille... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162791 Sat, 13 Nov 2010 18:55:17 +0100 4162791 http://www.medworm.com/index.php?rid=4162790&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21070169%26dopt%3DAbstract Authors: Pasquinelli G Plexiform fibrohistiocytic tumor (PFH) and cellular neurothekeoma (CN) are dermal neoplasms that present many histological and immunophenotype similarities as well as unknown histogenesis. Recently, the ultrastructural detection of zebra body-like inclusions in lesional histiocytes has been considered crucial to favor the diagnosis of PFH over that of CN. Here we report two examples of dermal tumors that were diagnosed as CN. Electron microscopy revealed cytoplasmic collections of myelin and zebra body-like inclusions in tumor cells; these inclusions focally merged together or with multivesicular bodies; tumor cells also showed collagen secretion granules and fibripositors, i.e., channels containing single, double or multiple copies of collagen fibrils. These obs... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4162790 Sat, 13 Nov 2010 18:55:09 +0100 4162790 http://www.medworm.com/index.php?rid=4073152&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20925598%26dopt%3DAbstract Authors: Perrotta I, Sciangula A, Perrotta E, Tripepi S, Donato G, Cassese M During diverse pathological conditions, vascular smooth muscle cells (SMCs) characteristically change from a quiescent, contractile phenotype to a proliferative, synthetic state, migrate toward the intima, and synthesize excess extracellular matrix. Although reactive oxygen species (ROS) are generally considered to be toxic to cells, recent evidence suggests that they may also modulate multiple signaling pathways. The vascular system contains several sources of ROS, among which NADPH oxidases (NOXes) have been shown to take an important part in the regulation of cell function, with effects on growth and proliferation. In the present study, the authors investigate the ultrastructural features of SMCs and the ex... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4073152 Wed, 06 Oct 2010 23:00:00 +0100 4073152 http://www.medworm.com/index.php?rid=4073151&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20925599%26dopt%3DAbstract Authors: Caruso RA, Fedele F, Crisafulli C, Paparo D, Parisi A, Lucian X00f2 R, Cavallari V The authors report a case of a 70-year-old woman with an anaplastic giant cell thyroid carcinoma, along with immunohistochemical and electron microscopic findings. Histologically, the tumor is characterized by mononucleated and multinucleated giant cells, lack of architectural cohesion, atypical mitoses, and extensive areas of coagulative necrosis. Tumor cells showed AE1/AE3 positivity as well as nuclear overexpression of p53 and ki-67. Semithin sections revealed multiple nuclei with heterogeneous size ranging from micronuclei to large-size (giant) nuclei. Micronuclei were confirmed by electron microscopy that disclosed also the presence of nuclear blebs, strings, and pockets. Morphological find... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4073151 Wed, 06 Oct 2010 23:00:00 +0100 4073151 http://www.medworm.com/index.php?rid=4073148&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20929309%26dopt%3DAbstract In this study the authors examined the ultrastructural differences between mucinous carcinoma and infiltrating ductal carcinoma NST. They also examined the protein expression of the tissues by 2D electrophoresis due to their belief that from the results of these two levels it is possible to understand the changes that take place both in the ultrastructural and biochemical levels in these two types of breast cancer. The ultrastructural results from mucinous carcinoma have shown many changes in cytoplasmic organelles in comparison to normal samples, depending on the grade and the number of metastatic lymph nodes. At the 2D elecrophoresis level the authors studied two interesting polypeptides, calreticulin and thioredoxin. Both of these proteins were found in patterns of fibroadenoma, mucinou... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4073148 Wed, 06 Oct 2010 23:00:00 +0100 4073148 http://www.medworm.com/index.php?rid=4073150&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20929307%26dopt%3DAbstract CONCLUSIONS: The authors believe this is the first report analyzing ultrastructual changes in normal human internal spermatic vein samples and in varicocele. The underlying molecular mechanisms of these changes await further studies. PMID: 20929307 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4073150 Thu, 30 Sep 2010 23:00:00 +0100 4073150 http://www.medworm.com/index.php?rid=4073149&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20929308%26dopt%3DAbstract We report a case of a 38 year old white male who presented with end stage renal disease of unknown etiology. He received a living-related donor kidney transplant (mother), but lost the graft after 10 years to multiple episodes of rejection. Review of the native renal biopsy with added ultrastructural studies established the diagnosis of Fabry's disease. Evaluation of renal biopsies showing advanced chronic injury should include electron microscopic studies, which may reveal characteristic diagnostic features, as seen in this case of Fabry's disease. Identification of hereditary disorders involving the kidney is important for appropriate treatment and prevention of disease recurrence. Potential living related donors should be screened for genetic involvement. PMID: 20929308 [PubMed - in... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=4073149 Thu, 30 Sep 2010 23:00:00 +0100 4073149 http://www.medworm.com/index.php?rid=3723855&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594037%26dopt%3DAbstract Authors: Johnson NB, Johnson MM, Selig MK, Nielsen GP The distinction between oncocytoma and chromophobe renal cell carcinoma, important clinically, may be challenging, especially as the tissue sample size decreases. Ancillary studies can be helpful, although subject to interpretation and sample variability. The aim of this study was to examine the value of electron microscopy in differentiating between oncocytoma and chromophobe renal cell carcinoma on formalin fixed paraffin embedded needle core biopsies. Twenty renal needle core biopsies were evaluated. Despite formalin fixation and paraffin embedding, the classic ultrastructural features of these neoplasms were retained, revealing 80% sensitivity and 100% specificity by initial work-up. PMID: 20594037 [PubMed - as supplied by p... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723855 Sun, 04 Jul 2010 19:45:39 +0100 3723855 http://www.medworm.com/index.php?rid=3723854&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594038%26dopt%3DAbstract This study demonstrates that proteinuria in IgA nephropathy is closely related to foot process effacement. PMID: 20594038 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723854 Sun, 04 Jul 2010 19:45:34 +0100 3723854 http://www.medworm.com/index.php?rid=3723853&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594039%26dopt%3DAbstract Authors: Jiang YS, Ehara T Trypsin digestion for 90 min revealed the alpha(5) chain of type IV collagen along the glomerular basement membrane and Bowman's capsule in paraffin-embedded renal sections of controls. In the 9 patients with the ultrastructures suggestive of Alport syndrome (AS), 8 patients were classified as X-linked dominant type due to the lack or mosaic pattern of alpha(5) chain in paraffin sections of renal biopsies by trypsin digestion, and 1 patient was classified as autosomol recessive type due to the lack of alpha(5) chain in the glomerular basement membrane only. Trypsin digestion is useful for the diagnosis of AS in paraffin-embedded renal tissue. PMID: 20594039 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723853 Sun, 04 Jul 2010 19:45:29 +0100 3723853 http://www.medworm.com/index.php?rid=3723852&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594040%26dopt%3DAbstract Authors: Perrotta I, Cristofaro MG, Amantea M, Russo E, De Fazio S, Zuccalà V, Conforti F, Amorosi A, Donato G, Tripepi S, Giudice M Osteonecrosis of the jaw is a severe bone disorder traditionally associated with periodontal disease, local malignancy, chemotherapy, glucocorticoid therapy, or trauma. Recently a growing number of publications reported the occurrence of osteonecrosis of the jaw in patients undergoing treatment with bisphosphonates. The mechanism by which bisphosphonates might contribute to the development of osteonecrosis of the jaw is far from being fully elucidated. Suppression of bone turnover, infection, tissue hypoxia and cellular toxicity were proposed as possible mechanisms by which bisphosphonates may exert adverse effects on bone metabolism. Here, we studied ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723852 Sun, 04 Jul 2010 19:45:26 +0100 3723852 http://www.medworm.com/index.php?rid=3723851&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594041%26dopt%3DAbstract Conclusions: Cutaneous degenerative microangiopathy is matched with cerebral microvascular pathology and could be important for the development of cognitive impairment in young adults with CKD. The characterization of microvascular pathology in skin biopsies, in this type of patients, could contribute to the knowledge of some pathophysiological and therapeutical topics and possibly be useful in clinical setting. Added patients are needed to establish a complete characterization of microangiopathy. PMID: 20594041 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723851 Sun, 04 Jul 2010 19:45:23 +0100 3723851 http://www.medworm.com/index.php?rid=3723850&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594042%26dopt%3DAbstract Authors: Latha LY, Darah I, Kassim MJ, Sasidharan S The antibacterial activity of Vernonia cinerea (L.) extract was investigated using the broth dilution method. The extract showed a favorable antimicrobial activity against Pseudomonas aeruginosa with a minimum inhibition concentration (MIC) value of 3.13 mg/mL. V. cinerea extract at (1/2), 1, or 2 times the MIC significantly inhibited bacterial growth with a noticeable drop in optical density (OD) of the bacterial culture, thus confirming the antibacterial activity of the extract on P. aeruginosa. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated P. aeruginosa. The main abnormalities noted via SEM and TEM studies were the alt... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723850 Sun, 04 Jul 2010 19:45:20 +0100 3723850 http://www.medworm.com/index.php?rid=3723849&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594043%26dopt%3DAbstract Authors: Krautgartner WD, Klappacher M, Hannig M, Obermayer A, Hartl D, Marcos V, Vitkov L Neutrophil extracellular traps (NETs) are extracellular web-like structures produced by activated polymorphonuclear neutrophils. NETs kill bacteria extracellularly, but their role in human pathology remains largely unclear. One possible way of studying NETs is through the SEM approach. However, web-like structures observed with SEM in sites of inflammation have been interpreted either as NETs or as fibrin. Thus, the question arises whether a reliable SEM discrimination between NETs and fibrin is at all possible. NET samples were collected as purulent crevicular exudate from periodontal pockets. DNase-digested controls for SEM were employed to demonstrate the DNA backbone and immuno-staining for c... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723849 Sun, 04 Jul 2010 19:45:17 +0100 3723849 http://www.medworm.com/index.php?rid=3723848&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594044%26dopt%3DAbstract Authors: Hussein MR, Al-Assiri M, Eid RA, Musalam AO Small cell neuroendocrine carcinoma (SCNEC) of the urinary bladder is a rare but aggressive neoplasm that usually exhibits neuroendocrine differentiation. Here, the authors report a case of SCNEC in an 80-year-old man. The patient had gross hematuria and nodular mass involving the wall of the urinary bladder. Total cystectomy was done. The tumor consisted of small, uniform, round, and spindled-shaped cells with chromatin dark nuclei and numerous mitotic figures. The cells were reactive for chromogranin, neuron-specific enolase (diffuse), and keratin (focal). Ultrastructural studies revealed neurosecretory granules and intermediate filaments. The diagnosis of SCNEC with focal high-grade urothelial component was established. No metasta... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723848 Sun, 04 Jul 2010 19:45:13 +0100 3723848 http://www.medworm.com/index.php?rid=3723847&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594045%26dopt%3DAbstract Authors: Pretorius E, Oberholzer HM, van der Spuy WJ, Meiring JH Smoking impacts on hemostasis and coagulation physiology is affected. Although this is well known, no previous research is available on the impact of smoking on fibrin network morphology. Here the authors show that smoking causes the fibrin network to have a netlike appearance in some areas, as well as areas where thick plaques are present. They argue that even in occasional smokers, fibrin, in the presence of thrombin, forms thickened areas that might be the cause of a thrombotic event such as stroke. Furthermore, it seems as if smoking impacts immediately on the fibrin architecture, and this therefore does not happen only over an extended period of smoking exposure. This information is important, particularly for women ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723847 Sun, 04 Jul 2010 19:45:10 +0100 3723847 http://www.medworm.com/index.php?rid=3723846&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20594046%26dopt%3DAbstract Authors: Brčić I, Brčić L, Kuzmanić D, Corić M, Corić M Fibronectin glomerulopathy is an inherited non-imune-mediated glomerulopathy associated with the massive deposition of fibronectin. It presents with proteinuria, microscopic hematuria, and hypertension that lead to end-stage renal failure in the second to sixth decade of life. A 34-year-old male was referred with proteinuria (6 g/day), microscopic hematuria and hypertension (220/130 mmHg). Renal biopsy specimen showed massive deposits of fibronectin in the mesangium and subendothelial spaces. After 20 months of multidrug treatment his renal function is stable. PMID: 20594046 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3723846 Sun, 04 Jul 2010 19:45:07 +0100 3723846 http://www.medworm.com/index.php?rid=3699762&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568982%26dopt%3DAbstract Authors: Xu X, Eyden B, Hou W, Chen T Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gut. It is characterized by positive immunostaining for CD117, and bears mutations in the c-kit or PDGFRA genes. Its origin remains uncertain. GISTs mainly possess primitive smooth muscle or neuronal differentiation. Although an epithelioid pattern of GIST is a common finding on light microscopy, true epithelial differentiation has never been demonstrated by either immunohistochemistry or ultrastructural study. Here the authors report an epithelioid GIST of the stomach, immunopositive for CD117, DOG1.1, CD34, and PDGFRA, with slight cytoplasmic staining for epithelial membrane antigen. One heterozygous mutation on codon 842 of exon 18 of the PDGFRA gene was also found... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699762 Tue, 22 Jun 2010 23:00:00 +0100 3699762 http://www.medworm.com/index.php?rid=3699761&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568983%26dopt%3DAbstract Authors: Nunes ET, Furquim KC, Bechara GH, Camargo-Mathias MI The ultrastructure of the salivary glands of Rhipicephalus (Boophilus) microplus females is described during feeding. In beginning of feeding, individuals show acini I with many mitochondria and wide basal labyrinth in peripheral cells; glycoprotein granules only in b and c(3) cells (acini II); and epithelial interstitial cells with developed basal labyrinth between f cells (acini III). Semi-engorged females show cells in degeneration, with autophagic vacuoles, lysosomes, myelin figures, and irregular, condensed, and/or fragmented nuclei, in addition to apoptotic bodies. R. B. microplus points to apoptosis in these organs before the detachment from the host, in contrast to others tick species. PMID: 20568983 [PubMed - as... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699761 Tue, 22 Jun 2010 23:00:00 +0100 3699761 http://www.medworm.com/index.php?rid=3699760&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568984%26dopt%3DAbstract Authors: Sharma SG, Spencer T, Gokden N IGA nephropathy (IGAN) is the most common glomerular disease worldwide. Patients may present with hematuria and non-nephrotic (NNRP) or uncommonly nephrotic range proteinuria (NRP). To the authors' knowledge, correlation of podocyte foot process effacement (FPE) with subclasses of IGAN and proteinuria (PT) has not been studied. Retrospectively, 161 cases of IGAN with light, immunofluorescence, and electron microscopy (EM) were reviewed and classified according to Haas classification. EM was available in 110 out of 161 (67%) cases. FPE was evaluated as mild, <30%; moderate, 30-70%; and severe, >70% and was correlated with class and the level of PT. Out of 161 cases, 101 were males and 60 were females with M:F ratio of 1.71:1. In 72 cases, ra... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699760 Tue, 22 Jun 2010 23:00:00 +0100 3699760 http://www.medworm.com/index.php?rid=3699759&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568985%26dopt%3DAbstract Authors: Arismendi-Morillo G, Castellano-Ramírez A, Medina Z The aim of this study was to describe the ultrastructural features of macrophage-like mononuclear leukocytes associated with human astrocytic tumors. Tumoral biopsies of 10 patients with a pathological diagnosis of astrocytic tumor by means of transmission electron microscopy were examined. The macrophage-like mononuclear leukocyte shows ultrastructural characteristics related with the physiologic phenotype of the alternatively activated macrophage (M2), localized principally around of tumoral vasculature and tumor milieu; classically activated macrophages (M1) in surrounding necrosis areas were observed. The presence of these two ultrastructural kinds of macrophage-like mononuclear leukocytes into different areas of the t... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699759 Tue, 22 Jun 2010 23:00:00 +0100 3699759 http://www.medworm.com/index.php?rid=3699758&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568987%26dopt%3DAbstract Authors: Rumessen JJ, Vanderwinden JM, Horn T Interstitial cells of Cajal (ICC) are key regulatory cells in the gut. In the colon of patients with severe ulcerative colitis (UC), myenteric ICC had myoid ultrastructural features and were in close contact with nerve terminals. In all patients as opposed to controls, some ICC profiles showed degenerative changes, such as lipid droplets and irregular vacuoles. Nerve terminals often appeared swollen and empty. Glial cells, muscle cells, and fibroblast-like cells (FLC) showed no alterations. FLC enclosed macrophages (MLC), which were in close contact with naked axon terminals. The organization and cytological changes may be of pathophysiological significance in patients with UC. PMID: 20568987 [PubMed - as supplied by publisher] (Source:... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699758 Tue, 22 Jun 2010 23:00:00 +0100 3699758 http://www.medworm.com/index.php?rid=3699757&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568988%26dopt%3DAbstract Conclusion: When adequate material is obtained, EM can contribute significantly to the evaluation of both FNA and exfoliative cytology cases, including the diagnosis and subclassification of epithelial and mesenchymal tumors, non-neoplastic processes, and the identification of microorganisms. PMID: 20568988 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699757 Tue, 22 Jun 2010 23:00:00 +0100 3699757 http://www.medworm.com/index.php?rid=3699756&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568989%26dopt%3DAbstract Authors: Papa V, Tarantino L, Preda P, Badiali De Giorgi L, Fanin M, Pegoraro E, Angelini C, Cenacchi G Storage diseases (SDs) are rare metabolic disorders characterized by the intra- or extralysosomal accumulation of unmetabolized compounds. Different causes determine the buildup of undigested material, resulting in typical histochemical and ultrastructural changes. Ultrastructural examination of tissue from patients with clinically suspected SDs may disclose pathognomonic alterations or suggest a differential diagnosis even in the absence of clinically evident involvement of the biopsied tissue. Accurate diagnosis of SDs requires a continuous integration of clinical, biochemical, ultrastructural, and, when available, molecular data. It is also important for the pathologist to be fami... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699756 Tue, 22 Jun 2010 23:00:00 +0100 3699756 http://www.medworm.com/index.php?rid=3699755&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20568990%26dopt%3DAbstract Authors: Eşrefoğlu M, Ara C Recently the authors have reported the potent beneficial effect of caffeic acid phenethyl ester (CAPE) on cholestatic oxidative liver injury induced by acute bile ligation in Swiss albino rats. Herein, they report the ultrastructural hepatocellular alterations induced by acute bile duct ligation and the effect of CAPE administration on these alterations. Bile duct ligation resulted in many degenerative changes, such as vacuolization, mitochondrial degeneration, endoplasmic reticulum dilatation, and lysosome accumulation within the cytoplasm of hepatocytes. Mitochondrial degeneration was also observed within the cytoplasm of the cells of biliary ductular epithelium. CAPE potentially protected the hepatocytes from the cholestasis-induced cellular injury.... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3699755 Tue, 22 Jun 2010 23:00:00 +0100 3699755 http://www.medworm.com/index.php?rid=3560757&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455658%26dopt%3DAbstract Authors: Tucker JA PMID: 20455658 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560757 Fri, 30 Apr 2010 23:00:00 +0100 3560757 http://www.medworm.com/index.php?rid=3560756&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455659%26dopt%3DAbstract Authors: Kloukina-Pantazidou I, Havaki S, Chrysanthou-Piterou M, Kontaxakis VP, Papadimitriou GN, Issidorides MR Studies of peripheral blood leukocytes of schizophrenic patients have shown in electron microscopy (EM) that decondensation of the chromatin constitutes a biological marker indicating increased genomic expression. Since this increase depends on chromatin relaxation by dissociation of lysine-rich histone H1 from nucleosomes, with exposure of arginine residues of core histones, the ratio of arginine to lysine residues in each nucleus represents a reliable measure of activation. Lysine- and arginine-rich proteins are demonstrable in light microscopy (LM), differentially, as yellow and black, respectively, with the ammoniacal silver reaction (ASR). Application of ASR on leukocyt... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560756 Fri, 30 Apr 2010 23:00:00 +0100 3560756 http://www.medworm.com/index.php?rid=3560755&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455660%26dopt%3DAbstract Authors: Iancu TC, Manov I Although there has been substantial progress in the identification of diarrheal diseases in infancy and childhood, electron microscopy may be still required for establishing diagnosis, staging, and response to therapy. This review describes severe conditions in which histopathologic examination alone cannot provide a firm diagnosis needed for therapeutic decisions. Microvillus inclusion disease, in its several variants, typifies this category. In certain forms of congenital disorders of glycosylation with gastrointestinal involvement, electron microscopic diagnosis is helpful. Among disorders due to abnormal immune-mediated reactions, celiac disease and cow's milk protein intolerance show fine structural changes of both diagnostic and staging value. Likewise,... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560755 Fri, 30 Apr 2010 23:00:00 +0100 3560755 http://www.medworm.com/index.php?rid=3560754&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455661%26dopt%3DAbstract In conclusion, both ERT and HCT are capable of restoring, at least partially, dermal fibroblast morphology in MPS I. PMID: 20455661 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560754 Fri, 30 Apr 2010 23:00:00 +0100 3560754 http://www.medworm.com/index.php?rid=3560753&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455662%26dopt%3DAbstract Authors: Horvath E, Coire CI, Kovacs K, Smyth HS Two unique, formerly unrecorded sellar neoplasms were observed in two women of 60 and 63 years of age. One lesion consisted of small epithelial cells and the other was a large-cell oncocytic tumor, yet they had the same simple cytoplasmic organization with dominance of polyribosomes and a sprinkle of glycogen. Striking markers shared by the neoplasms: (1) network of typical pituitary follicles, and (2) unexpected similarity to fetal human pituitary tissue at different gestational ages of 6 and 10-12 weeks. The latter showed appreciable endocrine differentiation. The assumed parent cell is the folliculo-stellate cell as pluripotent adult stem cell. PMID: 20455662 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560753 Fri, 30 Apr 2010 23:00:00 +0100 3560753 http://www.medworm.com/index.php?rid=3560752&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455663%26dopt%3DAbstract This report confirms and complements previous observations about this new mode of tumor cell death. It supports the contention that a combination of VC+VK(3), also named Apatone, could be co-administered as a nontoxic adjuvant with radiation and/or chemotherapies to kill bladder tumor cells and other cancer cells without any supplementary risk or side effects for patients. PMID: 20455663 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560752 Fri, 30 Apr 2010 23:00:00 +0100 3560752 http://www.medworm.com/index.php?rid=3560751&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455664%26dopt%3DAbstract Authors: Herrera GA, Turbat-Herrera EA Renal dysfunction is often seen in patients with plasma cell dyscrasias. The abnormal light and heavy chains that are produced by the neoplastic plasma cells in these patients are responsible for the renal abnormalities that occur. The renal manifestations are heterogeneous and include alterations in all three renal compartments; sometimes more than one compartment is affected in a given case. It must be demonstrated that the renal abnormalities are directly related to the underlying plasma cell dyscrasia to make a definitive diagnosis of an associated lesion. Therefore, it becomes crucial to link the renal findings with the circulating nephrotoxic light or heavy chains. Immunofluorescence is very helpful and diagnostic in the majority of the case... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560751 Fri, 30 Apr 2010 23:00:00 +0100 3560751 http://www.medworm.com/index.php?rid=3560750&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20455665%26dopt%3DAbstract Authors: Min KW Gastrointestinal stromal tumor (GIST) is the most frequent spindle cell tumor in the gastrointestinal tract and may arise from esophagus to rectum. The stomach is the most frequent site, followed by small intestine, rectum, and esophagus. There have been some regional differences reported in their histopathologic and clinical presentations. The purpose of this study is to compare ultrastructural features of GIST, according to its anatomic site, in order to provide additional data to support the current concept of its histogenesis. Fifty-four GISTs (27 from stomach, 23 from small intestine, and 4 from rectum) were included in the study. Histopathologically, gastric GISTs tended to be more frequently epithelioid, particularly those in children, while small intestinal GIST... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3560750 Fri, 30 Apr 2010 23:00:00 +0100 3560750 http://www.medworm.com/index.php?rid=3327426&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192700%26dopt%3DAbstract In conclusion, the results showed that the ultrastructural study provides essential or helpful information in many cases of glomerular diseases, and therefore electron microscopy should be considered an important tool of diagnostic renal pathology. As was recommended, it is important to reserve renal tissue for ultrastructural study unless electron microscopy can be routinely used in all biopsies. Thus, this technique could be performed wherever a renal biopsy has to be ultrastructurally evaluated. PMID: 20192700 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327426 Wed, 03 Mar 2010 18:17:26 +0100 3327426 http://www.medworm.com/index.php?rid=3327425&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192701%26dopt%3DAbstract Authors: Mansy SS, Elkhafif NA, Abelfatah AS, Yehia HA, Mostafa I An ultrastructural quantitative assessment of hepatic stellate cells (HSCs) was made in relation to hepatic fibrosis, apoptotic cellular changes, intracellular fat deposition, circulating inflammatory cells in the sinusoids, and the necroinflammatory activity in liver specimens of 33 patients proven to be positive for hepatitis C virus (HCV)-RNA by polymerase chain reaction with the intention that electron microscopy may throw more light on the role of HSCs in the complicated process of fibrogenesis. A detailed review concerning these parameters and observed evidence suggesting the potential properties of HSCs to recycle cellular debris into collagen fibers are reported. PMID: 20192701 [PubMed - in process] (Source: ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327425 Wed, 03 Mar 2010 18:17:18 +0100 3327425 http://www.medworm.com/index.php?rid=3327424&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192702%26dopt%3DAbstract Conclusion: Combined clinical and ultrastructural examination has led to a definitive diagnosis. These diseases exhibit indolent progression and as yet do not have specific treatment. PMID: 20192702 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327424 Wed, 03 Mar 2010 18:17:10 +0100 3327424 http://www.medworm.com/index.php?rid=3327423&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192703%26dopt%3DAbstract In this study, cell surface projections of primary culture cells from tissues of infiltrating ductal carcinoma Non Special Type with vascular invasion are examined by use of the Scanning Electron Microscopy method. In these cases the projections of cell membrane appeared extremely long and bridge-like covering very long distances between the breast cancer cells. Also, the long cell membrane projections, connect cells between them and form a complex. Sometimes, from one edge to another we observed a very long chain of cancer cells reaching sometimes a length of 3, 3 mm. On the other hand the absence of vascular invasion never shows such long projections of the cell membrane even if there are many metastatic nodes. The role of these extra long projections in communication between cancer cell... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327423 Wed, 03 Mar 2010 18:17:01 +0100 3327423 http://www.medworm.com/index.php?rid=3327422&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192704%26dopt%3DAbstract In this study of 2 adult patients with presumptive mitochondrial disease, the authors have identified distinctive morphological changes in medial rectus muscle biopsies that confirm the diagnosis of chronic progressive external ophthalmoplegia (CPEO). These findings demonstrate the usefulness of electron microscopy using medial rectus muscle in the diagnosis of adult patients with a slowly progressive course of mild skeletal weakness and CPEO. PMID: 20192704 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327422 Wed, 03 Mar 2010 18:16:52 +0100 3327422 http://www.medworm.com/index.php?rid=3327421&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192705%26dopt%3DAbstract In this study cerebral ultrastructure was examined in an in vivo rat model, after rewarming from profound hypothermia (15-13 degrees C). Animals held at 37 degrees C served as controls. After rewarming, brains were examined by electron microscope. Micrographs were taken randomly, analyzed anonymously, and quantified by morphometry. Serum analysis of the stress marker S-100beta was carried out in identical groups. The most striking findings in rewarmed animals, when compared to controls, were alterations of myelin sheaths (p<.008) and elevated S-100beta (p<.0001). This indicates that cells in the central nervous system are susceptible to injury in an experimental model of accidental hypothermia and rewarming. PMID: 20192705 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327421 Wed, 03 Mar 2010 18:16:44 +0100 3327421 http://www.medworm.com/index.php?rid=3327420&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192706%26dopt%3DAbstract Authors: Gilloteaux J, Ekwedike NN Comparisons of atrial tissues from Syrian hamster offspring born from cocaine-treated mothers during the last days of pregnancy with sham-treated ones demonstrate irreversible focal ischemic damage in the Purkinje myofibers and minor endocardial damages as well as minute cardiomyocyte vacuolization. These defects are consistent with the pharmacotoxicity of cocaine or its metabolites. The damaged Purkinje myocytes apparently remain in contact with adjacent cardiomyocytes but undergo autolytic process similar to that found in autoschizic cell death. Adjacent cell type(s) appear to segregate or engulf the injured cells. Data collected in this report demonstrate why clinical bradyarrhythmias, arrhythmias, or sudden death as cardiac arrest can be found in ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327420 Wed, 03 Mar 2010 18:16:34 +0100 3327420 http://www.medworm.com/index.php?rid=3327419&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192707%26dopt%3DAbstract Authors: Wang G, Eyden B Sclerosing epithelioid fibrosarcoma (SEF) is a rare variant of fibrosarcoma, described initially by Meis-Kindblom et al. in 1995 (Meis-Kindblom JM, Kindblom L-G, Enzinger FM. Sclerosing epithelioid fibrosarcoma: a variant of fibrosarcoma simulating carcinoma. Am J Surg Pathol. 1995;19:979-993): more than 80 cases have been documented clinicopathologically since. Bone is a rare primary site for SEF, with only 2 cases so far reported. This paper documents the detailed clinical, histological, immunohistochemical, and ultrastructural features of a case occurring in the pubic bone of a 57-year-old diabetic woman presenting with a history of pain and compromised mobility involving her hip. Radiology revealed a destructive lesion in the right pubic bone. The lesion wa... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327419 Wed, 03 Mar 2010 18:16:25 +0100 3327419 http://www.medworm.com/index.php?rid=3176469&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070146%26dopt%3DAbstract Authors: Nesland JM, Johannessen JV PMID: 20070146 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176469 Sat, 16 Jan 2010 00:56:30 +0100 3176469 http://www.medworm.com/index.php?rid=3176468&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070147%26dopt%3DAbstract Authors: Pitassi LH, Cintra ML, Ferreira MR, Magalhães RF, Velho PE Some Bartonella species are able to invade red blood cells (RBC) and may cause persistent infection in the susceptible host. Use of transmission electron microscopy (TEM) demonstrates, inside erythrocytes, the typical triple-walled agents. However, when examining ultrathin sections of blood cells, the authors have, on several occasions, detected intraerythrocytic abnormalities that mimic but are not typical of Bartonella spp. Small endovesicles, pseudoinclusions, cavities, and irregular hemoglobin granules distribution, resulting in regions of increased or decreased electron density, may be observed in the erythrocytes and platelets, which may be confused with bartonellas. So far, detailed ultrastructural findings... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176468 Sat, 16 Jan 2010 00:56:25 +0100 3176468 http://www.medworm.com/index.php?rid=3176467&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070148%26dopt%3DAbstract Authors: Crincoli V, Di Bisceglie MB, Scivetti M, Favia A, Di Comite M Dens invaginatus (D.I.) is a developmental anomaly caused by the infolding of the surface of a tooth crown before calcification has occurred. Its aetiology is controversial and remains unclear. It occurs in all dentitions with a prevalence that ranges from 0.25% to 7.74% and is mostly seen in the maxillary permanent incisors, particularly in the lateral incisors. Posterior teeth are infrequently involved. The purpose of this study was to investigate the morpho-structure of a second upper molar dens invaginatus compared with a control tooth. Ground and decalcified sections were prepared and histo-morphological evaluation of dental tissues was performed by using light microscopy, microradiography, and confocal laser s... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176467 Sat, 16 Jan 2010 00:56:21 +0100 3176467 http://www.medworm.com/index.php?rid=3176466&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070149%26dopt%3DAbstract Authors: Gupta M, de Leval L, Selig M, Oliva E, Nielsen GP Uterine tumors resembling ovarian sex cord tumors (UTROSCT) are tumors of unclear histogenesis. The authors analyzed the ultrastructural features of 13 UTROSCT and correlated the findings with their immunohistochemical profile. Features included cells with frequent organoid, nested or cord-like arrangement (8), lumen formation (2; one of which showed surface microvilli), nuclei with irregular indentations (8), intermediate filaments (13), prominent paranuclear aggregates (5), cell junctions (9), desmosome-like junctions (2), tonofilaments (2), basal lamina (1), and cytoplasmic lipid droplets (7; prominent in 3). No dense bodies, subplasmalemmal densities or pinocytotic vesicles were seen. Ultrastructural epithelial differentiat... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176466 Sat, 16 Jan 2010 00:56:18 +0100 3176466 http://www.medworm.com/index.php?rid=3176465&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070150%26dopt%3DAbstract Authors: Vitkov L, Klappacher M, Hannig M, Krautgartner WD The fate of the neutrophils within the inflammatory exudate in the periodontal crevice and their possible participation in the formation of neutrophil extracellular traps (NETs) are of clinical interest. However, the cytological analysis of clinical samples of inflammatory exudate is restricted by the obtainable quantities, which do not enable employing the routine approaches. Clinical examinations, ACLAR strip sampling, scanning electron microscopy, and confocal laser scanning microscopy were employed to analyze purulent crevicular exudate and gingival crevicular fluid in periodontitis. Bacteria, neutrophil activation, NETosis stages, and NETs were identified by molecular probe, expression of citrullinated histone H3, enzymati... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176465 Sat, 16 Jan 2010 00:56:14 +0100 3176465 http://www.medworm.com/index.php?rid=3176464&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070151%26dopt%3DAbstract Authors: Kravic-Stevovic T, Bogdanovic A, Boskovic D, Bumbasirevic V The ribosome-lamella complex (RLC) is a cylindrical structure composed of different numbers of circular lamellae with associated particles, regarded as ribosomes, around a central core. Structures resembling RLC, but lacking the typical mature appearance of RLC, have been called pre-RLC. The authors have found RLCs and pre-RLCs in peripheral lymphocytes of 3 patients with chronic lymphocytic leukemia (CLL). The fact that CLL patients with RLCs were in early Rai clinical stages, had good clinical prognostic factors, and did not require immediate therapy indicates that RLCs occurred in the early course of some cases of CLL. Moreover, the presence of RLC was associated with hypogammaglobulinemia M. PMID: 20070151 [Pu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176464 Sat, 16 Jan 2010 00:56:11 +0100 3176464 http://www.medworm.com/index.php?rid=3176463&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070152%26dopt%3DAbstract CONCLUSIONS: Diabetic Yorkshire pigs develop characteristic features of an early retinal microvasculopathy fairly rapidly and may serve as a higher-order animal model for studies of type 1 diabetes. PMID: 20070152 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176463 Sat, 16 Jan 2010 00:56:08 +0100 3176463 http://www.medworm.com/index.php?rid=3176462&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070153%26dopt%3DAbstract Authors: Eyden B Rhabdomyosarcoma has traditionally been subclassified into alveolar, embryonal, and pleomorphic variants. Less commonly, spindle-cell, neuroendocrine, sclerosing, and lipid-rich or clear-cell subtypes are seen. The author recently encountered a myogenic sarcoma, with all the common markers of rhabdomyosarcoma, but expressing the unusual features of alpha-smooth-muscle actin and abundant rough endoplasmic reticulum (rER). This myogenic sarcoma, therefore, exhibited four lines of differentiation, and is documented here. The patient was a 65-year-old man with an inguinal soft tissue mass. Following surgical excision, the patient was given radiotherapy and was well without disease after 6 years. The tumor was positive for vimentin, desmin, alpha-smooth-muscle actin, alpha-... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176462 Sat, 16 Jan 2010 00:56:05 +0100 3176462 http://www.medworm.com/index.php?rid=3030609&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929171%26dopt%3DAbstract This study analyzed the midgut of unfed, partially engorged, and fully engorged fed females as well as three infestations in rabbits. In A. cajennense, the digestive tube is long and from the midgut, two pairs of diverticula ramify and lead to a blind end. In some midgut regions were observed for the first time in ticks, structures termed here "nodules." The midgut of unfed females possesses a pseudostratified epithelium composed of digestive and generative cells. In partially engorged and engorged females at 1st infestation and partially engorged at 2nd infestation, the epithelium becomes stratified. In partially engorged females at 2nd infestation, the epithelium exhibits a third cell type: secretory cell. So the intestinal epithelium undergoes several changes during the feeding process ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030609 Thu, 26 Nov 2009 23:16:21 +0100 3030609 http://www.medworm.com/index.php?rid=3030608&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929172%26dopt%3DAbstract Authors: Mubarak M, Kazi JI ABSTRACT To determine the role of immunofluorescence (IF) and electron microscopy (EM) in the evaluation of renal biopsies in a developing country, the authors carried out a study in 200 patients with nephrotic syndrome. Renal biopsies were studied by light microscopy, IF, and EM. IF study was useful in all, being essential in 23.5% and helpful in remaining cases. EM was useful in 94.5% cases, being essential in 43% and helpful in 51.5% cases. The results demonstrate that IF and EM are essential in the evaluation of renal biopsies in nephrotic syndrome and these should be employed in the pathologic evaluation of renal biopsies. PMID: 19929172 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030608 Thu, 26 Nov 2009 23:16:19 +0100 3030608 http://www.medworm.com/index.php?rid=3030607&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929173%26dopt%3DAbstract Authors: Eşrefoǧlu M, Gül M, Seyhan M, Selimoǧlu MA ABSTRACT Renal, skeletal, cardiac, and ophthalmic involvement in Wilson disease (WD) is well known. In this case report, high copper content and ultrastructural findings of skin of a patient with WD accompanied by xerosis are presented. PMID: 19929173 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030607 Thu, 26 Nov 2009 23:16:16 +0100 3030607 http://www.medworm.com/index.php?rid=3030606&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929174%26dopt%3DAbstract This study provides morphological examples of skewed type II macrophage infiltration in gastric adenocarcinomas that is involved in scavenging activity, particularly erythrophagocytosis, formation of mature (nonepithelioid granulomas), and heterotypic aggregation with eosinophils. PMID: 19929174 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030606 Thu, 26 Nov 2009 23:16:13 +0100 3030606 http://www.medworm.com/index.php?rid=3030605&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929175%26dopt%3DAbstract Conclusion: SFT is an uncommon neoplasm with different histological patterns and clinical behavior. The authors hypothetize that the perivascular undifferentiated cells that most cases showed might correspond to a quiescent stage of adult stem mesenchymal cell and could be the target of the molecular aberrations implied in its pathogenesis. PMID: 19929175 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030605 Thu, 26 Nov 2009 23:16:10 +0100 3030605 http://www.medworm.com/index.php?rid=3030604&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929176%26dopt%3DAbstract Authors: Wartchow EP, Goin L, Schreiber J, Mierau GW, Terella A, Allen GC ABSTRACT Plexiform fibrohistiocytic tumor is a low-grade soft tissue malignancy that can at times be difficult to differentiate from the less biologically aggressive cellular neurothekeoma. The two entities, which may display identical clinical and histological features, cannot be distinguished by immunohistochemical or molecular diagnostic means. Electron microscopy may enable the accurate identification of problematic examples and thus aid in resolving these occasionally occurring diagnostic dilemmas. To illustrate typical variations in the ultrastructural appearance of plexiform fibrohistiocytic tumor, the authors present two diagnostically noncontroversial examples, and to demonstrate the potential diagnostic... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030604 Thu, 26 Nov 2009 23:16:08 +0100 3030604 http://www.medworm.com/index.php?rid=3030603&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929177%26dopt%3DAbstract Authors: Pasquinelli G, Foroni L, Papadopoulos F, Dicandia L, Bisceglia M ABSTRACT Superficial acral fibromyxoma (SAFM) is an uncommon tumor of the superficial soft tissues of acral sites. SAFM is a proliferation of fibroblastic cells, within a myxoid to collagenous stroma. The published cases mostly expressed immunoreactivity for CD34, CD99, EMA, and, less frequently, CD10. The authors report an additional case that did not express any of the previously reported markers, including CD34, and antigens of mesenchymal stromal lineage. Ultrastructural study confirmed the tumor cells were typical fibroblasts with cytoplasmic intermediate filaments and numerous cisternae of rough endoplasmic reticulum. The authors describe the first example of SAFM, ultrastructurally studied, with pure fibro... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030603 Thu, 26 Nov 2009 23:16:05 +0100 3030603 http://www.medworm.com/index.php?rid=2981905&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895290%26dopt%3DAbstract In conclusion, there are morphological differences in the apical region of primary teeth with different pulpal and periapical pathologies. PMID: 19895290 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981905 Thu, 01 Oct 2009 00:00:00 +0100 2981905 http://www.medworm.com/index.php?rid=2981904&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895291%26dopt%3DAbstract Authors: Mahringer C, Fureder M, Kastner M, Ebner A, Hinterdorfer P, Vitkov L, Hannig M, Kienberger F, Schilcher K Atomic force microscopy (AFM) was used to study the effects of bleaching on the morphology of the enamel surface with nanoscale resolution. Samples of human tooth enamel with native (pumiced) or fine-polished surfaces were examined before and after bleaching with 30% carbamide peroxide. The obtained profilometric AFM data revealed significant morphological surface alterations. After 1 h of bleaching, the surface roughness increased significantly from 19 +/- 4nm to 33 +/- 5 nm. Six-hour bleaching did not produce any significant further increase in enamel surface roughness. The interrod junction depth raised more than twice after 1 h of bleaching. After 6 h of bleaching, a f... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981904 Thu, 01 Oct 2009 00:00:00 +0100 2981904 http://www.medworm.com/index.php?rid=2981903&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895292%26dopt%3DAbstract This study investigated the ultrastructural conformation changes of the chromatin in blood leukocytes of bipolar patients, versus normal controls, by using the phosphotungstic acid-hematoxylin (PTAH) block-staining method, modified for electron microscopy, and the immunohistochemical localization of the histone H1, by the immunogold method. These two methods are basically complementary. If histone H1 immunolabeling is used, it shows that the immunogold labeling on chromatin is different in the three phases of the illness, i.e., high in normothymia and low in depression as well as in mania. However, in this particular tissue fixation (4% paraformaldehyde-1% glutaraldehyde in 0,1 M phosphate buffer), the heterochromatin in the nuclei remains identical in the three phases of the illness. On t... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981903 Thu, 01 Oct 2009 00:00:00 +0100 2981903 http://www.medworm.com/index.php?rid=2981902&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895293%26dopt%3DAbstract Authors: Mattar EH, Haffor AS Hyperoxia has been widely used as model for oxidative stress. Free radicals (FR), suproxide anion (*O(-)(2)) and nitric oxide anion (*NO(-)), are highly toxic and have detrimental effects on nitroso-redox balance in the myocyte. Myocardium is rich with beta-adrenergic receptors and endothelial is the site of NO production. The authors hypothesized that graded doses of dobutamine result in hyperkinetic state, which shifts the nitroso-redox balance toward the buildup of reactive species in dose-dependent excess. The purpose of the present study was to investigate free radicals production and coronary endothelial cell pathological changes following increasing length of breathing oxygen (100% O(2)) and progressive doses of dobutamine. Thirty-five adult male ra... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981902 Thu, 01 Oct 2009 00:00:00 +0100 2981902 http://www.medworm.com/index.php?rid=2981901&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895294%26dopt%3DAbstract Authors: Pretorius E, Oberholzer HM, van der Spuy WJ, Meiring JH Macrothrombocytopenia is a rare condition where large, circulating platelets ranging between approximately 5 and 20 microm are found (typically platelets size range from 1.5 to 2.5 microm). The condition is also characterized by the prevalence of decreased numbers of circulating platelets, bleeding, short circulating times in blood, as well as abnormal platelet destruction. The current research investigates the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) of platelet aggregates and fibrin networks of a family diagnosed with macrothrombocytopenia. Although TEM analysis of macrothrombocytopenia is not novel, little is known regarding the SEM analysis of platelet aggregates and fibrin network... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981901 Thu, 01 Oct 2009 00:00:00 +0100 2981901 http://www.medworm.com/index.php?rid=2981900&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895295%26dopt%3DAbstract Authors: Singh HK, Donna Thompson B, Nickeleit V Haufen, i.e. discrete three-dimensional cast-like polyomavirus aggregates in the urine, mark polyoma BK-virus nephropathy (BKN) with positive and negative predictive values of greater than 95%. They are novel diagnostic biomarkers of BKN, an important infectious complication post kidney transplantation. Here, we describe technical details of Haufen detection by negative staining electron microscopy. We studied more than 400 urine samples from over 180 patients and developed easy-to-follow protocols for optimal specimen preservation and preparation, including sample clarification and concentration. We detail diagnostic clues to detect Haufen and illustrate pitfalls, including "Haufen-look-alikes," which can hamper the interpretation. Urin... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981900 Thu, 01 Oct 2009 00:00:00 +0100 2981900 http://www.medworm.com/index.php?rid=2981899&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895296%26dopt%3DAbstract Authors: Ru YX, Mao BY, Zhang FK, Pang TX, Zhao SX, Liu JH, Wickramasinghe SN Severe malarial anemia causes considerable mortality and morbidity in endemic areas. Possible mechanisms underlying the anemia include lysis of parasitized and nonparasitized red cells as well as parasite product-mediated effects on erythropoiesis. The latter include suppression of erythropoiesis, dyserythropoiesis, and ineffective erythropoiesis. Present transmission electron microscope data in two cases of Pasmodium vivax malaria show a hitherto undescribed mechanism contributing to malarial anemia, namely, infection of erythroblasts by parasites and their subsequent degradation. No parasites were detected in the peripheral blood but parasites were found in the bone marrow. These findings emphasise the valu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981899 Thu, 01 Oct 2009 00:00:00 +0100 2981899 http://www.medworm.com/index.php?rid=2981898&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895297%26dopt%3DAbstract Authors: Cangiotti AM, Morroni M Ribosome-lamella complexes (RLCs) are mainly observed in a variety of hematological disorders and occasionally in solid neoplasms and in nonneoplastic diseases. These intracytoplasmic organelles are held to arise from rough endoplasmic reticulum, but, in agreement with more recent literature data, their function is still unclear. Ultrastructural analysis of glomeruli from a patient with focal segmental glomerulosclerosis secondary to metabolic syndrome disclosed significant foot process loss and abundant cytoskeletal proteins in major podocyte processes; two of the latter also displayed RLCs. This is the second report of RLCs in human renal glomerulus. Their close association with cytoskeletal proteins and lysosomes suggests a relationship with abnormal... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981898 Thu, 01 Oct 2009 00:00:00 +0100 2981898 http://www.medworm.com/index.php?rid=2765632&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728229%26dopt%3DAbstract This study uses gene expression profiling by quantitative real time polymerase chain reaction (qRT-PCR) to identify "intrinsic" subtypes in a Saudi population of breast cancers and compares the distribution of subtypes to the more commonly profiled Caucasian population. In addition, the immunohistochemical profile of breast cancers was correlated to the gene expression analysis. Discrepancy rate of 39% in subtype prediction between gene expression and immunohistochemical profile of the tumors was noticed. Most of this variation was in the luminal subtype. Frequency of HER2+ subtype in the Saudi cases was high (28%) by both the immunohistochemistry (IHC) and the qRT-PCR classification. Triple-negative tumors comprised 39% while only 11% showed a basal-like profile. Analysis of larger cohort... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765632 Tue, 30 Jun 2009 23:00:00 +0100 2765632 http://www.medworm.com/index.php?rid=2765631&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728230%26dopt%3DAbstract Authors: Magalhães RF, Urso Pitassi LH, Lania BG, Barjas-Castro ML, Neves Ferreira Velho PE The authors present the case of a young man with aplastic anemia who went into shock and died after several red blood cell unit transfusions. Immunohematological studies did not show any abnormality and blood cultures from patients and blood bags were negative. The ultrastructural findings, allied with current scientific knowledge, permitted the diagnosis of Bartonella sp. infection. In face of this diagnosis, two possibilities should be considered: the first one is that the patient was already infected by the bacteria before the last RBC unit transfusion. The pathogen could be involved in aplastic anemia etiology and in the failure to recover hemoglobin levels, in spite of the transfusions... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765631 Tue, 30 Jun 2009 23:00:00 +0100 2765631 http://www.medworm.com/index.php?rid=2765630&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728231%26dopt%3DAbstract In conclusion, this study reports the fine definition of pulse granuloma at microscopic level by CLSM and the results warrant the use of this technique for further analyses. PMID: 19728231 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765630 Tue, 30 Jun 2009 23:00:00 +0100 2765630 http://www.medworm.com/index.php?rid=2765629&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728232%26dopt%3DAbstract Authors: Liu Y, Geng L, Suo Z The human pro-monocytic leukemia U937 cell line was previously reported to become rho degrees cells after a long-term ethidium bromide exposure. In the authors' extensive PCR studies with different pairs of primers for the mtDNA molecule they showed that these U937-rho degrees cells, after being cultured in their laboratory for a time, did replete their mtDNA. That the cells grew well in the normal medium (RPMI 1640 plus 10% fetal calf serum and 2 mM l-glutamine) as the parental cells also suggests that these cells contain functional mitochondria and mtDNA molecules. Further experiments showed that the cells cultured in the medium with pyruvate and uridine rather rapidly and strongly adhered on the culture flask walls while the cells cultured in the medium... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765629 Tue, 30 Jun 2009 23:00:00 +0100 2765629 http://www.medworm.com/index.php?rid=2765628&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728233%26dopt%3DAbstract Authors: Rampisela D, Donner LR The authors report the first case of perineurioma of the adrenal gland. The tumor was composed of elongated wavy spindle cells focally arranged in a fascicular pattern. It was positive for epithelial membrane antigen (EMA) and claudin-1, and was negative for S-100 protein and glial fibrillary acidic protein (GFAP). Electron microscopy showed long, slender cytoplasmic processes coated by discontinuos basal lamina and presence of many pinocytotic vesicles. PMID: 19728233 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765628 Tue, 30 Jun 2009 23:00:00 +0100 2765628 http://www.medworm.com/index.php?rid=2765627&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728234%26dopt%3DAbstract Authors: Guimaraes Fde S, de Oliveira SM, de Oliveira CC, Donatti L, Buchi Dde F The performance of a moderately shorter fixation protocol for transmission electron microscopy (TEM) was evaluated by analyzing the cell structure quality after the processing. The relevance of this experimental technique is mainly based on reducting time of the steps of conventional protocols: fixation, washes, dehydration, and epoxy resin infiltration. Two sources of murine cells were used, the peritoneal and mesenteric lymph node cells. A fixation and material processing faster than usual methods can save time and improve results. Samples analysis indicated good preservation of different cell structures and organelles after this protocol. PMID: 19728234 [PubMed - in process] (Source: Ultrastructural... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765627 Tue, 30 Jun 2009 23:00:00 +0100 2765627 http://www.medworm.com/index.php?rid=2765626&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728235%26dopt%3DAbstract In this study, the authors aimed to disclose whether the hoechst33342 staining required extensive optimization for identifying SP cells in the human ovarian cancer cell line OVCAR-3. After systematic evaluations, it was found that only 2.5 microg/mL hoechst33342 staining of the cells for 60 min could get an ideal SP population, which accounted for 0.9% of the whole cell population. The sorted SP cells showed significantly higher colony formation efficiency than the non-side population (NSP) cells, and only the SP cells could form holoclones. Real-time PCR disclosed that SP cells expressed higher levels of "stemness" gene Oct3/4 than the NSP cells did, indicating that the SP cells might harbor cancer stem cells in this cell line. The results highlight the necessity of SP method optimization... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765626 Tue, 30 Jun 2009 23:00:00 +0100 2765626 http://www.medworm.com/index.php?rid=2538903&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479649%26dopt%3DAbstract Authors: Potgieter M, Pretorius E, Oberholzer HM The numerous protective effects of coenzyme Q10 (CoQ10) evoked the question of whether it might be able to elicit protection to cell membranes after being challenged by the membrane disrupter Triton X-100. Cardiac and skeletal muscle tissue from chick embryos was cultured and exposed to increasing concentrations of CoQ10 and Triton X-100. Scanning electron microscopy was used to study cell morphology. Results suggested the ability of CoQ10 to offer protection to cells challenged by Triton X-100. The authors suggest that CoQ10 may offer protection to muscle cells, by enhancing membrane repair via patch formation by an unknown mechanism that possibly involves Ca(2+)-dependent ion channel activation. PMID: 19479649 [PubMed - in process]... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538903 Sat, 27 Jun 2009 21:36:02 +0100 2538903 http://www.medworm.com/index.php?rid=2538902&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479650%26dopt%3DAbstract Authors: Castejon OJ In a vascular anomaly showing moderate edema, the extracellular space appeared apparently normal, exhibiting a membrane to membrane space of about 20 nm in width. In congenital hydrocephalus, this space appeared notably enlarged and occupied by an electron transparent, nonproteinaceous interstitial edema fluid, due to abnormal accumulation of cerebrospinal fluid. In brain trauma, the distended extracellular space contained either electron-lucid nonproteinaceous or electron-dense proteinaceous edema fluid. Hemorrhagic foci, fibrinoid material, and non-nervous invading cells, such as macrophages and monocytes, were also found. In brain tumors, the widened extracellular space showed electron-dense proteinaceous edema fluid and bundles of fibrinoid material. The enlarg... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538902 Sat, 27 Jun 2009 21:36:02 +0100 2538902 http://www.medworm.com/index.php?rid=2538901&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479651%26dopt%3DAbstract Authors: Bin-Jaliah I, Dallak M, Haffor AS Hyperoxia (HP) exposure inducts reactive oxygen species (ROS) in the lungs that may result in lung injury, including alveolar epithelial and endothelial cells. Lactate dehydrogenase (LDH) activity relates to glycolysis, whereas glutathione peroxidase (Gpx) activity relies on the pentose phosphate pathway (PPP). The purpose of this study was to examine early ROS-induced alveolar pathological changes in relation to the activity of glutathione peroxidase (GPx) and lactate dehydrogenase (LDH) activity. Twenty adult male rats, matched with age and body weight, were randomly assigned to two groups, control and experimental. The experimental group was exposed to hyperoxia for 24 h. Ultrastructure examination showed degenerated pneumocyte type I, cont... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538901 Sat, 27 Jun 2009 21:36:02 +0100 2538901 http://www.medworm.com/index.php?rid=2538900&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479652%26dopt%3DAbstract Authors: Garcia CA, Abell-Aleff PC, Gamb SI, Miller DV Amyloidomas are localized mass-forming deposits of amyloid that occur with or without association with systemic amyloidosis. The ultrastructural findings in 3 amyloidomas from 2 autopsy patients with primary systemic AL amyloidosis are described. By transmission electron microscopy, there were randomly oriented nonbranching fibrils showing some unusual curvilinear forms and considerable variability in fibril diameter (two subsets of fibrils, one 12-14 nm and another 28-30 nm in diameter). The larger fibrils showed features of microtubule formation. Scanning electron microscopy demonstrated complex 3-dimensional tangles of fibrils. These findings add to the current ultrastructural and morphologic spectrum of paraprotein deposition d... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538900 Sat, 27 Jun 2009 21:36:02 +0100 2538900 http://www.medworm.com/index.php?rid=2538899&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479653%26dopt%3DAbstract Authors: Sipponen A, Peltola R, Jokinen JJ, Laitinen K, Lohi J, Rautio M, Mannisto M, Sipponen P, Lounatmaa K Resin salve prepared from Norway spruce (Picea abies) has been used for centuries in traditional medicine to treat skin diseases. The authors studied with transmission and scanning electron microscopy, and with electron physiology, changes in cell wall and cell membrane of Staphylococcus aureus after exposure of the bacterial cultures to resin. After exposure, cell wall thickening, cell aggregation, changed branching of fatty acids, and dissipation of membrane potential of the bacterial cells were observed. The authors conclude that spruce resin affects the cell viability via changes in the cell wall and membrane, and impairs, thereby, the synthesis of energy in the bacteria. ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538899 Sat, 27 Jun 2009 21:36:02 +0100 2538899 http://www.medworm.com/index.php?rid=2538898&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479654%26dopt%3DAbstract Authors: Ru YX, Eyden B, Li QH, Mao BY, Liu EB, Pang TX Primary and metastatic carcinomas have a reactive stroma characterized by many myofibroblasts. These cells have also been documented in nonepithelial malignancies, such as sarcomas, malignant melanoma, and lymphoid tumors but in generally far fewer numbers. In non-Hodgkin's lymphoma, Hodgkin's disease, and leukemia, myofibroblasts are rather rarely documented. In particular, there appear to be no reports of myofibroblasts in either primary bone-marrow/peripheral blood leukemia or secondary deposits of leukemia. In this paper, a case of a relapsed chronic myeloid leukemia appearing in an inguinal lymph node is described, containing many myofibroblasts. The case is detailed and presented with a discussion on the role of myofibroblas... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538898 Sat, 27 Jun 2009 21:36:02 +0100 2538898 http://www.medworm.com/index.php?rid=2253505&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274579%26dopt%3DAbstract Authors: Al-Johany AM, Haffor AS Animal studies on the toxicity of heavy metals have been widely used as model to simulate the impacts of environmental pollution on the human health. In the present study the authors hypothesized that cadmium exposure inducts changes in reactive oxygen species (ROS) and that may be involved in the pathogenesis of lung diseases. The pathological changes of different pulmonary cells of ROS-cadmium-dependent effects were investigated in relation to the activity of lactate dehydrogenase (LDH) and glutathione peroxidase (GPx). Twelve animals were randomly assigned to two groups, control and experimental. The experimental group underwent ingestion of cadmium mixed with diet (200 mg/kg) for 7 weeks. Following the treatment conditions for each group, blood samp... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253505 Sun, 01 Mar 2009 05:00:00 +0100 2253505 http://www.medworm.com/index.php?rid=2253504&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274580%26dopt%3DAbstract Authors: Scivetti M, Lucchese A, Crincoli V, Pilolli GP, Favia G Liesegang rings are concentric noncellular lamellar structures, occasionally found in inflammatory tissues. They have been confused with various parasites, algas, calcification, and psammoma bodies. The authors examined Liesegang rings from oral inflammatory cysts by both optical and confocal laser scanning microscopy, and perfomed a three-dimensional reconstruction. These investigations indicate that Liesegang rings are composed of multiple birefringent concentric rings, resulting from a progressive deposition of organic substances, with an unclear pathogenesis. PMID: 19274580 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253504 Sun, 01 Mar 2009 05:00:00 +0100 2253504 http://www.medworm.com/index.php?rid=2253503&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274581%26dopt%3DAbstract Authors: Kang D, Kunugi S, Masuda Y, Ishizaki M, Koizumi K, Fukuda Y Three cases of biphasic mesothelioma and 2 cases of sarcomatoid mesothelioma were investigated using light and electron microscopy. In 2 of the 3 cases of biphasic mesotheliomas, fibrous long-spacing (FLS) collagen fibrils were discovered with a symmetrical cross-striation of 130 nm in periodicity. However, no connection between the FLS fibrils and usual collagen fibrils were observed. Periodic acid silver methenamine stain revealed unstained bands with periods of 130 nm in FLS fibrils, whereas the usual collagen fibrils showed continuous positive staining. All 3 cases of biphasic mesotheliomas showed deposits of hyaluronic acid, whereas both cases of sarcomatoid mesotheliomas showed little hyaluronic acid. As a high ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253503 Sun, 01 Mar 2009 05:00:00 +0100 2253503 http://www.medworm.com/index.php?rid=2253502&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274582%26dopt%3DAbstract Authors: Eyden B, Wang G, Yao L Epithelioid sarcoma is an uncommon but well-described malignancy, which is found predominantly in the soft tissues of the young and middle-aged, and which pursues an indolent to aggressive course. It shows a degree of both mesenchymal and epithelial differentiation. Myofibroblastic differentiation has been recorded in epithelioid sarcoma for some time, the evidence being based mainly on the presence of smooth-muscle-type myofilaments and, more recently, on alpha-smooth-muscle actin and muscle-specific actin immunostaining. Myofibroblastic differentiation based on the stricter criterion of the fibronectin fibril/fibronexus junction has not so far been demonstrated except for a single atypical case with spindle-cell morphology and a cytokeratin-negative im... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253502 Sun, 01 Mar 2009 05:00:00 +0100 2253502 http://www.medworm.com/index.php?rid=2253501&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274583%26dopt%3DAbstract The objective of this paper is to produce an ultrastructural classification of acute monocytic leukemia (AML-M5) in relation to clinical behaviors. The ultrastructural characteristics of blasts of the monocytic series were analyzed in 72 M5 patients by transmission electron microscopy (TEM), in terms of their content of typical monoblasts, atypical monoblasts, atypical promonocytes, and typical promonocytes in bone-marrow aspirates. Four kinds of monocytic blasts were identified by cell size and shape, nuclear profile, nucleocytoplasmic ratio, heterochromatin content, nucleolus, granules, vesicles, and Golgi apparatus. Their characteristics of remission rate, cytochemistry, immunophenotype, and cytogenetics were also investigated. The data obtained permitted M5 patients to be divided into ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253501 Sun, 01 Mar 2009 05:00:00 +0100 2253501 http://www.medworm.com/index.php?rid=2253500&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274584%26dopt%3DAbstract Authors: Walpusk J, Galambos C Despite the widespread utilization of immunohistochemical stains in pathologic practice, virus identification remains a challenge. In our institution, electron microscopy combined with the lift technique has been utilized for 40 years as a reliable diagnostic tool where a question of viral infection is raised by light microscopic observation and could not be otherwise confirmed. The combination of light microscopic and ultrastructural methods has allowed us to examine individual cells suspicious for harboring viral particles previously identified on a hematoxylin-eosin stained tissue section. In this review we describe the lift technique in detail with our modifications in the hope that the use of lift technique in these uncommon but specific circumstance... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253500 Sun, 01 Mar 2009 05:00:00 +0100 2253500 http://www.medworm.com/index.php?rid=2253498&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274585%26dopt%3DAbstract Authors: Toumpanaki A, Baltatzis GE, Gaitanarou E, Seretis E, Toumpanakis C, Aroni K, Kittas C, Voloudakis-Baltatzis IE The aim of the present study was to observe possible qualitative and quantitative expression differences between nuclear matrix proteins (NMPs) of human colon adenocarcinoma and their mirror biopsies, using the technique of two-dimensional gel electrophoresis, in order to identify the existence of specific NMP fingerprints for colon cancer. Colon tissues were examined ultrastructurally and NMPs were isolated biochemically, by serial extraction of lipids, soluble proteins, DNA, RNA, and intermediate filaments and were separated according to their isoelectric point (pI) and their molecular weight (MW) by high-resolution two-dimensional electrophoresis (2D). By comparing... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253498 Sun, 01 Mar 2009 05:00:00 +0100 2253498 http://www.medworm.com/index.php?rid=2162637&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191195%26dopt%3DAbstract In this study, 4 cases of human gastric carcinomas with massive neutrophil infiltration were studied by light and electron microscopy, focusing on the inflammatory angiogenesis in the tumor stroma. At light microscopy, the tumors were advanced gastric carcinomas in which various degrees of tubular differentiation were present. Under an electron microscope, pericytes exhibited two major differentiated states with distinct ultrastructural features: a contractile phenotype and a synthetic phenotype. The contractile phenotype was characterized by abundant microfilaments. Synthetic pericytes contained abundant rough endoplasmic reticulum, lipid bodies, and numerous membrane-bound vesicles. These ultrastructural findings extend concept of contractile/synthetic phenotype modulation, originally de... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162637 Thu, 01 Jan 2009 05:00:00 +0100 2162637 http://www.medworm.com/index.php?rid=2162636&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191196%26dopt%3DAbstract Authors: Kaarteenaho-Wiik R, Paakko P, Sormunen R Fibroblast differentiation into myofibroblast in transforming growth factor-beta1-exposed human lung fibroblasts and the immunolocalizations of alpha-smooth muscle actin, fibronectin, tenascin-C, and osteopontin in exposed cells were studied by conventional transmission electron microscopy and immunoelectron microscopy. Ultrastructural features of myofibroblasts were detected after exposure, e.g., alpha-smooth muscle actin positive bundles in the cytoplasm of cells and extracellular fibronectin-containing structures on the surface of the cell forming fibronexus structure, osteopontin adjacent to rough endoplastic reticulum and extracellular tenascin-C in the vicinity of the cell. The authors concluded that exposure to transforming growt... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162636 Thu, 01 Jan 2009 05:00:00 +0100 2162636 http://www.medworm.com/index.php?rid=2162635&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191197%26dopt%3DAbstract Authors: Eyden B Basal/external lamina is not found over plasma cells or other hemolymphoid cells, and the feature can have diagnostic value in distinguishing the neoplastic counterparts of such cells from epithelium, endothelium, mesothelium, and so on, which do have this feature. In this paper, a material ultrastructurally indistinguishable from basal or external lamina is reported on reactive plasma cells found in a fibrous pseudotumor, intralobular stroma of normal breast, tumor stroma of squamous cell carcinoma, and submucosa of normal human small intestine. It was focal, followed the contours of the cell-surface membrane, was lightly textured, 40-80 nm thick, and separated from the plasma cell surface membrane by a clear space resembling a lamina lucida. Its function remains unce... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162635 Thu, 01 Jan 2009 05:00:00 +0100 2162635 http://www.medworm.com/index.php?rid=2162634&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191198%26dopt%3DAbstract Authors: Mansy SS, Abdelfatah AS, Hassanein MH The present work highlights intracellular viral morphogenesis and virus-host cell interactions in patients proved to be infected with HCV. The material of this study consisted of 28 liver biopsies taken from patients positive for serum HCV-RNA by polymerase chain reaction. Liver biopsies were processed for light and electron microscopic examination. Ultrastructural findings of this work supported a new hypothesis for the turnover of HCV to retrovirus and described the presumed involved mechanism. This novel perception offers important insights that can explain the vague mechanisms of HCV behavior in the infected hepatocytes. PMID: 19191198 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162634 Thu, 01 Jan 2009 05:00:00 +0100 2162634 http://www.medworm.com/index.php?rid=2162633&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191199%26dopt%3DAbstract Authors: Eyden B The fibronexus is recognized as a characteristic marker of the myofibroblast. However, it is not completely specific for this cell, having been seen in aortic smooth muscle (in attenuated form) and endothelium in experimental animals. This paper documents fibronexus junctions in human in vivo endothelium. Ultrastructural observations were made on the vasculature of a desmoplastic and focally neurotropic malignant melanoma. Cross-sectioned fibronectin fibrils were seen outside the stromal surface of the endothelial plasmalemma. Often, they were positioned directly opposite the actin-filament bundles in the peripheral cytoplasm. Neoplastic and in vitro cultured cells apart, endothelium is the only nonmyofibroblastic cell type to show well-developed fibronexus junctions. ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162633 Thu, 01 Jan 2009 05:00:00 +0100 2162633 http://www.medworm.com/index.php?rid=2162632&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191200%26dopt%3DAbstract Authors: Eyden B, Chakrabarty B, Hatimy U Lymphoma diagnosis rarely needs electron microscopy (EM), but one area where it can be useful is in the distinction of cytokeratin-positive lymphoma from carcinoma. The authors describe such a case, where difficulties were encountered due to lack of antibody specificity, distinguishing reactive from tumoral cells, and suboptimal sampling for EM. The tumor was in a lymph node next to the right submandibular gland in a 69-year-old man. This was a malignant tumor, composed of sheets of monomorphic large round cells. Interpretation on the part of a team of pathologists who examined this tumor was divided. On histological sections, the differential diagnosis was between carcinoma and lymphoma, which was modified to cytokeratin-positive lymphoma vers... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162632 Thu, 01 Jan 2009 05:00:00 +0100 2162632 http://www.medworm.com/index.php?rid=2071892&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117263%26dopt%3DAbstract Authors: Zouvani I, Aristodemou S, Hadjisavvas A, Michael T, Vassiliou M, Patsias C, Pierides A, Ioannou K, Kyriacou K Thin basement membrane nephropathy is one of the main causes of hematuria in both children and adults. It is often associated with a family history and its true incidence is unknown. Accurate diagnosis of thin basement membrane nephropathy relies on the presence of attenuated glomerular basement membranes, a finding that can be appreciated only by examination in the electron microscope. In Cyprus the department of electron microscopy has received 990 consecutive renal biopsies for diagnosis. The aim of this study is to define the incidence of thin basement membrane nephropathy in this population sample based on the results of electron microscopy. PMID: 19117263 [Pu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071892 Sat, 01 Nov 2008 04:00:00 +0100 2071892 http://www.medworm.com/index.php?rid=2071891&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117264%26dopt%3DAbstract Authors: Davidson B Malignant mesothelioma is a primary cancer of the serosal cavities, an anatomic site that is also frequently affected by metastatic disease, predominantly from primary carcinomas of the lung, breast, and ovary. Advances in immunohistochemistry have resulted in improved diagnostic sensitivity and specificity in the differential diagnosis between metastatic adenocarcinoma and malignant mesothelioma in both cytological and histological material. Recently, the author's group applied high throughput technology to the identification of new markers that may aid in differentiating malignant mesothelioma from ovarian and peritoneal serous carcinoma, tumors with closely related histogenesis and antigenic profile. In addition to the improved tools available for serosal cancer ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071891 Sat, 01 Nov 2008 04:00:00 +0100 2071891