MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Ultrastructural Pathology' source. Thu, 11 Mar 2010 15:56:08 +0100 http://www.medworm.com/index.php?rid=3327426&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192700%26dopt%3DAbstract In conclusion, the results showed that the ultrastructural study provides essential or helpful information in many cases of glomerular diseases, and therefore electron microscopy should be considered an important tool of diagnostic renal pathology. As was recommended, it is important to reserve renal tissue for ultrastructural study unless electron microscopy can be routinely used in all biopsies. Thus, this technique could be performed wherever a renal biopsy has to be ultrastructurally evaluated. PMID: 20192700 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327426 Wed, 03 Mar 2010 18:17:26 +0100 3327426 http://www.medworm.com/index.php?rid=3327425&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192701%26dopt%3DAbstract Authors: Mansy SS, Elkhafif NA, Abelfatah AS, Yehia HA, Mostafa I An ultrastructural quantitative assessment of hepatic stellate cells (HSCs) was made in relation to hepatic fibrosis, apoptotic cellular changes, intracellular fat deposition, circulating inflammatory cells in the sinusoids, and the necroinflammatory activity in liver specimens of 33 patients proven to be positive for hepatitis C virus (HCV)-RNA by polymerase chain reaction with the intention that electron microscopy may throw more light on the role of HSCs in the complicated process of fibrogenesis. A detailed review concerning these parameters and observed evidence suggesting the potential properties of HSCs to recycle cellular debris into collagen fibers are reported. PMID: 20192701 [PubMed - in process] (Source: ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327425 Wed, 03 Mar 2010 18:17:18 +0100 3327425 http://www.medworm.com/index.php?rid=3327424&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192702%26dopt%3DAbstract Conclusion: Combined clinical and ultrastructural examination has led to a definitive diagnosis. These diseases exhibit indolent progression and as yet do not have specific treatment. PMID: 20192702 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327424 Wed, 03 Mar 2010 18:17:10 +0100 3327424 http://www.medworm.com/index.php?rid=3327423&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192703%26dopt%3DAbstract In this study, cell surface projections of primary culture cells from tissues of infiltrating ductal carcinoma Non Special Type with vascular invasion are examined by use of the Scanning Electron Microscopy method. In these cases the projections of cell membrane appeared extremely long and bridge-like covering very long distances between the breast cancer cells. Also, the long cell membrane projections, connect cells between them and form a complex. Sometimes, from one edge to another we observed a very long chain of cancer cells reaching sometimes a length of 3, 3 mm. On the other hand the absence of vascular invasion never shows such long projections of the cell membrane even if there are many metastatic nodes. The role of these extra long projections in communication between cancer cell... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327423 Wed, 03 Mar 2010 18:17:01 +0100 3327423 http://www.medworm.com/index.php?rid=3327422&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192704%26dopt%3DAbstract In this study of 2 adult patients with presumptive mitochondrial disease, the authors have identified distinctive morphological changes in medial rectus muscle biopsies that confirm the diagnosis of chronic progressive external ophthalmoplegia (CPEO). These findings demonstrate the usefulness of electron microscopy using medial rectus muscle in the diagnosis of adult patients with a slowly progressive course of mild skeletal weakness and CPEO. PMID: 20192704 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327422 Wed, 03 Mar 2010 18:16:52 +0100 3327422 http://www.medworm.com/index.php?rid=3327421&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192705%26dopt%3DAbstract In this study cerebral ultrastructure was examined in an in vivo rat model, after rewarming from profound hypothermia (15-13 degrees C). Animals held at 37 degrees C served as controls. After rewarming, brains were examined by electron microscope. Micrographs were taken randomly, analyzed anonymously, and quantified by morphometry. Serum analysis of the stress marker S-100beta was carried out in identical groups. The most striking findings in rewarmed animals, when compared to controls, were alterations of myelin sheaths (p<.008) and elevated S-100beta (p<.0001). This indicates that cells in the central nervous system are susceptible to injury in an experimental model of accidental hypothermia and rewarming. PMID: 20192705 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327421 Wed, 03 Mar 2010 18:16:44 +0100 3327421 http://www.medworm.com/index.php?rid=3327420&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192706%26dopt%3DAbstract Authors: Gilloteaux J, Ekwedike NN Comparisons of atrial tissues from Syrian hamster offspring born from cocaine-treated mothers during the last days of pregnancy with sham-treated ones demonstrate irreversible focal ischemic damage in the Purkinje myofibers and minor endocardial damages as well as minute cardiomyocyte vacuolization. These defects are consistent with the pharmacotoxicity of cocaine or its metabolites. The damaged Purkinje myocytes apparently remain in contact with adjacent cardiomyocytes but undergo autolytic process similar to that found in autoschizic cell death. Adjacent cell type(s) appear to segregate or engulf the injured cells. Data collected in this report demonstrate why clinical bradyarrhythmias, arrhythmias, or sudden death as cardiac arrest can be found in ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327420 Wed, 03 Mar 2010 18:16:34 +0100 3327420 http://www.medworm.com/index.php?rid=3327419&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20192707%26dopt%3DAbstract Authors: Wang G, Eyden B Sclerosing epithelioid fibrosarcoma (SEF) is a rare variant of fibrosarcoma, described initially by Meis-Kindblom et al. in 1995 (Meis-Kindblom JM, Kindblom L-G, Enzinger FM. Sclerosing epithelioid fibrosarcoma: a variant of fibrosarcoma simulating carcinoma. Am J Surg Pathol. 1995;19:979-993): more than 80 cases have been documented clinicopathologically since. Bone is a rare primary site for SEF, with only 2 cases so far reported. This paper documents the detailed clinical, histological, immunohistochemical, and ultrastructural features of a case occurring in the pubic bone of a 57-year-old diabetic woman presenting with a history of pain and compromised mobility involving her hip. Radiology revealed a destructive lesion in the right pubic bone. The lesion wa... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3327419 Wed, 03 Mar 2010 18:16:25 +0100 3327419 http://www.medworm.com/index.php?rid=3176469&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070146%26dopt%3DAbstract Authors: Nesland JM, Johannessen JV PMID: 20070146 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176469 Sat, 16 Jan 2010 00:56:30 +0100 3176469 http://www.medworm.com/index.php?rid=3176468&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070147%26dopt%3DAbstract Authors: Pitassi LH, Cintra ML, Ferreira MR, Magalhães RF, Velho PE Some Bartonella species are able to invade red blood cells (RBC) and may cause persistent infection in the susceptible host. Use of transmission electron microscopy (TEM) demonstrates, inside erythrocytes, the typical triple-walled agents. However, when examining ultrathin sections of blood cells, the authors have, on several occasions, detected intraerythrocytic abnormalities that mimic but are not typical of Bartonella spp. Small endovesicles, pseudoinclusions, cavities, and irregular hemoglobin granules distribution, resulting in regions of increased or decreased electron density, may be observed in the erythrocytes and platelets, which may be confused with bartonellas. So far, detailed ultrastructural findings... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176468 Sat, 16 Jan 2010 00:56:25 +0100 3176468 http://www.medworm.com/index.php?rid=3176467&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070148%26dopt%3DAbstract Authors: Crincoli V, Di Bisceglie MB, Scivetti M, Favia A, Di Comite M Dens invaginatus (D.I.) is a developmental anomaly caused by the infolding of the surface of a tooth crown before calcification has occurred. Its aetiology is controversial and remains unclear. It occurs in all dentitions with a prevalence that ranges from 0.25% to 7.74% and is mostly seen in the maxillary permanent incisors, particularly in the lateral incisors. Posterior teeth are infrequently involved. The purpose of this study was to investigate the morpho-structure of a second upper molar dens invaginatus compared with a control tooth. Ground and decalcified sections were prepared and histo-morphological evaluation of dental tissues was performed by using light microscopy, microradiography, and confocal laser s... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176467 Sat, 16 Jan 2010 00:56:21 +0100 3176467 http://www.medworm.com/index.php?rid=3176466&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070149%26dopt%3DAbstract Authors: Gupta M, de Leval L, Selig M, Oliva E, Nielsen GP Uterine tumors resembling ovarian sex cord tumors (UTROSCT) are tumors of unclear histogenesis. The authors analyzed the ultrastructural features of 13 UTROSCT and correlated the findings with their immunohistochemical profile. Features included cells with frequent organoid, nested or cord-like arrangement (8), lumen formation (2; one of which showed surface microvilli), nuclei with irregular indentations (8), intermediate filaments (13), prominent paranuclear aggregates (5), cell junctions (9), desmosome-like junctions (2), tonofilaments (2), basal lamina (1), and cytoplasmic lipid droplets (7; prominent in 3). No dense bodies, subplasmalemmal densities or pinocytotic vesicles were seen. Ultrastructural epithelial differentiat... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176466 Sat, 16 Jan 2010 00:56:18 +0100 3176466 http://www.medworm.com/index.php?rid=3176465&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070150%26dopt%3DAbstract Authors: Vitkov L, Klappacher M, Hannig M, Krautgartner WD The fate of the neutrophils within the inflammatory exudate in the periodontal crevice and their possible participation in the formation of neutrophil extracellular traps (NETs) are of clinical interest. However, the cytological analysis of clinical samples of inflammatory exudate is restricted by the obtainable quantities, which do not enable employing the routine approaches. Clinical examinations, ACLAR strip sampling, scanning electron microscopy, and confocal laser scanning microscopy were employed to analyze purulent crevicular exudate and gingival crevicular fluid in periodontitis. Bacteria, neutrophil activation, NETosis stages, and NETs were identified by molecular probe, expression of citrullinated histone H3, enzymati... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176465 Sat, 16 Jan 2010 00:56:14 +0100 3176465 http://www.medworm.com/index.php?rid=3176464&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070151%26dopt%3DAbstract Authors: Kravic-Stevovic T, Bogdanovic A, Boskovic D, Bumbasirevic V The ribosome-lamella complex (RLC) is a cylindrical structure composed of different numbers of circular lamellae with associated particles, regarded as ribosomes, around a central core. Structures resembling RLC, but lacking the typical mature appearance of RLC, have been called pre-RLC. The authors have found RLCs and pre-RLCs in peripheral lymphocytes of 3 patients with chronic lymphocytic leukemia (CLL). The fact that CLL patients with RLCs were in early Rai clinical stages, had good clinical prognostic factors, and did not require immediate therapy indicates that RLCs occurred in the early course of some cases of CLL. Moreover, the presence of RLC was associated with hypogammaglobulinemia M. PMID: 20070151 [Pu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176464 Sat, 16 Jan 2010 00:56:11 +0100 3176464 http://www.medworm.com/index.php?rid=3176463&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070152%26dopt%3DAbstract CONCLUSIONS: Diabetic Yorkshire pigs develop characteristic features of an early retinal microvasculopathy fairly rapidly and may serve as a higher-order animal model for studies of type 1 diabetes. PMID: 20070152 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176463 Sat, 16 Jan 2010 00:56:08 +0100 3176463 http://www.medworm.com/index.php?rid=3176462&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20070153%26dopt%3DAbstract Authors: Eyden B Rhabdomyosarcoma has traditionally been subclassified into alveolar, embryonal, and pleomorphic variants. Less commonly, spindle-cell, neuroendocrine, sclerosing, and lipid-rich or clear-cell subtypes are seen. The author recently encountered a myogenic sarcoma, with all the common markers of rhabdomyosarcoma, but expressing the unusual features of alpha-smooth-muscle actin and abundant rough endoplasmic reticulum (rER). This myogenic sarcoma, therefore, exhibited four lines of differentiation, and is documented here. The patient was a 65-year-old man with an inguinal soft tissue mass. Following surgical excision, the patient was given radiotherapy and was well without disease after 6 years. The tumor was positive for vimentin, desmin, alpha-smooth-muscle actin, alpha-... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3176462 Sat, 16 Jan 2010 00:56:05 +0100 3176462 http://www.medworm.com/index.php?rid=3030609&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929171%26dopt%3DAbstract This study analyzed the midgut of unfed, partially engorged, and fully engorged fed females as well as three infestations in rabbits. In A. cajennense, the digestive tube is long and from the midgut, two pairs of diverticula ramify and lead to a blind end. In some midgut regions were observed for the first time in ticks, structures termed here "nodules." The midgut of unfed females possesses a pseudostratified epithelium composed of digestive and generative cells. In partially engorged and engorged females at 1st infestation and partially engorged at 2nd infestation, the epithelium becomes stratified. In partially engorged females at 2nd infestation, the epithelium exhibits a third cell type: secretory cell. So the intestinal epithelium undergoes several changes during the feeding process ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030609 Thu, 26 Nov 2009 23:16:21 +0100 3030609 http://www.medworm.com/index.php?rid=3030608&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929172%26dopt%3DAbstract Authors: Mubarak M, Kazi JI ABSTRACT To determine the role of immunofluorescence (IF) and electron microscopy (EM) in the evaluation of renal biopsies in a developing country, the authors carried out a study in 200 patients with nephrotic syndrome. Renal biopsies were studied by light microscopy, IF, and EM. IF study was useful in all, being essential in 23.5% and helpful in remaining cases. EM was useful in 94.5% cases, being essential in 43% and helpful in 51.5% cases. The results demonstrate that IF and EM are essential in the evaluation of renal biopsies in nephrotic syndrome and these should be employed in the pathologic evaluation of renal biopsies. PMID: 19929172 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030608 Thu, 26 Nov 2009 23:16:19 +0100 3030608 http://www.medworm.com/index.php?rid=3030607&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929173%26dopt%3DAbstract Authors: Eşrefoǧlu M, Gül M, Seyhan M, Selimoǧlu MA ABSTRACT Renal, skeletal, cardiac, and ophthalmic involvement in Wilson disease (WD) is well known. In this case report, high copper content and ultrastructural findings of skin of a patient with WD accompanied by xerosis are presented. PMID: 19929173 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030607 Thu, 26 Nov 2009 23:16:16 +0100 3030607 http://www.medworm.com/index.php?rid=3030606&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929174%26dopt%3DAbstract This study provides morphological examples of skewed type II macrophage infiltration in gastric adenocarcinomas that is involved in scavenging activity, particularly erythrophagocytosis, formation of mature (nonepithelioid granulomas), and heterotypic aggregation with eosinophils. PMID: 19929174 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030606 Thu, 26 Nov 2009 23:16:13 +0100 3030606 http://www.medworm.com/index.php?rid=3030605&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929175%26dopt%3DAbstract Conclusion: SFT is an uncommon neoplasm with different histological patterns and clinical behavior. The authors hypothetize that the perivascular undifferentiated cells that most cases showed might correspond to a quiescent stage of adult stem mesenchymal cell and could be the target of the molecular aberrations implied in its pathogenesis. PMID: 19929175 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030605 Thu, 26 Nov 2009 23:16:10 +0100 3030605 http://www.medworm.com/index.php?rid=3030604&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929176%26dopt%3DAbstract Authors: Wartchow EP, Goin L, Schreiber J, Mierau GW, Terella A, Allen GC ABSTRACT Plexiform fibrohistiocytic tumor is a low-grade soft tissue malignancy that can at times be difficult to differentiate from the less biologically aggressive cellular neurothekeoma. The two entities, which may display identical clinical and histological features, cannot be distinguished by immunohistochemical or molecular diagnostic means. Electron microscopy may enable the accurate identification of problematic examples and thus aid in resolving these occasionally occurring diagnostic dilemmas. To illustrate typical variations in the ultrastructural appearance of plexiform fibrohistiocytic tumor, the authors present two diagnostically noncontroversial examples, and to demonstrate the potential diagnostic... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030604 Thu, 26 Nov 2009 23:16:08 +0100 3030604 http://www.medworm.com/index.php?rid=3030603&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19929177%26dopt%3DAbstract Authors: Pasquinelli G, Foroni L, Papadopoulos F, Dicandia L, Bisceglia M ABSTRACT Superficial acral fibromyxoma (SAFM) is an uncommon tumor of the superficial soft tissues of acral sites. SAFM is a proliferation of fibroblastic cells, within a myxoid to collagenous stroma. The published cases mostly expressed immunoreactivity for CD34, CD99, EMA, and, less frequently, CD10. The authors report an additional case that did not express any of the previously reported markers, including CD34, and antigens of mesenchymal stromal lineage. Ultrastructural study confirmed the tumor cells were typical fibroblasts with cytoplasmic intermediate filaments and numerous cisternae of rough endoplasmic reticulum. The authors describe the first example of SAFM, ultrastructurally studied, with pure fibro... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=3030603 Thu, 26 Nov 2009 23:16:05 +0100 3030603 http://www.medworm.com/index.php?rid=2981905&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895290%26dopt%3DAbstract In conclusion, there are morphological differences in the apical region of primary teeth with different pulpal and periapical pathologies. PMID: 19895290 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981905 Thu, 01 Oct 2009 00:00:00 +0100 2981905 http://www.medworm.com/index.php?rid=2981904&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895291%26dopt%3DAbstract Authors: Mahringer C, Fureder M, Kastner M, Ebner A, Hinterdorfer P, Vitkov L, Hannig M, Kienberger F, Schilcher K Atomic force microscopy (AFM) was used to study the effects of bleaching on the morphology of the enamel surface with nanoscale resolution. Samples of human tooth enamel with native (pumiced) or fine-polished surfaces were examined before and after bleaching with 30% carbamide peroxide. The obtained profilometric AFM data revealed significant morphological surface alterations. After 1 h of bleaching, the surface roughness increased significantly from 19 +/- 4nm to 33 +/- 5 nm. Six-hour bleaching did not produce any significant further increase in enamel surface roughness. The interrod junction depth raised more than twice after 1 h of bleaching. After 6 h of bleaching, a f... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981904 Thu, 01 Oct 2009 00:00:00 +0100 2981904 http://www.medworm.com/index.php?rid=2981903&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895292%26dopt%3DAbstract This study investigated the ultrastructural conformation changes of the chromatin in blood leukocytes of bipolar patients, versus normal controls, by using the phosphotungstic acid-hematoxylin (PTAH) block-staining method, modified for electron microscopy, and the immunohistochemical localization of the histone H1, by the immunogold method. These two methods are basically complementary. If histone H1 immunolabeling is used, it shows that the immunogold labeling on chromatin is different in the three phases of the illness, i.e., high in normothymia and low in depression as well as in mania. However, in this particular tissue fixation (4% paraformaldehyde-1% glutaraldehyde in 0,1 M phosphate buffer), the heterochromatin in the nuclei remains identical in the three phases of the illness. On t... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981903 Thu, 01 Oct 2009 00:00:00 +0100 2981903 http://www.medworm.com/index.php?rid=2981902&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895293%26dopt%3DAbstract Authors: Mattar EH, Haffor AS Hyperoxia has been widely used as model for oxidative stress. Free radicals (FR), suproxide anion (*O(-)(2)) and nitric oxide anion (*NO(-)), are highly toxic and have detrimental effects on nitroso-redox balance in the myocyte. Myocardium is rich with beta-adrenergic receptors and endothelial is the site of NO production. The authors hypothesized that graded doses of dobutamine result in hyperkinetic state, which shifts the nitroso-redox balance toward the buildup of reactive species in dose-dependent excess. The purpose of the present study was to investigate free radicals production and coronary endothelial cell pathological changes following increasing length of breathing oxygen (100% O(2)) and progressive doses of dobutamine. Thirty-five adult male ra... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981902 Thu, 01 Oct 2009 00:00:00 +0100 2981902 http://www.medworm.com/index.php?rid=2981901&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895294%26dopt%3DAbstract Authors: Pretorius E, Oberholzer HM, van der Spuy WJ, Meiring JH Macrothrombocytopenia is a rare condition where large, circulating platelets ranging between approximately 5 and 20 microm are found (typically platelets size range from 1.5 to 2.5 microm). The condition is also characterized by the prevalence of decreased numbers of circulating platelets, bleeding, short circulating times in blood, as well as abnormal platelet destruction. The current research investigates the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) of platelet aggregates and fibrin networks of a family diagnosed with macrothrombocytopenia. Although TEM analysis of macrothrombocytopenia is not novel, little is known regarding the SEM analysis of platelet aggregates and fibrin network... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981901 Thu, 01 Oct 2009 00:00:00 +0100 2981901 http://www.medworm.com/index.php?rid=2981900&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895295%26dopt%3DAbstract Authors: Singh HK, Donna Thompson B, Nickeleit V Haufen, i.e. discrete three-dimensional cast-like polyomavirus aggregates in the urine, mark polyoma BK-virus nephropathy (BKN) with positive and negative predictive values of greater than 95%. They are novel diagnostic biomarkers of BKN, an important infectious complication post kidney transplantation. Here, we describe technical details of Haufen detection by negative staining electron microscopy. We studied more than 400 urine samples from over 180 patients and developed easy-to-follow protocols for optimal specimen preservation and preparation, including sample clarification and concentration. We detail diagnostic clues to detect Haufen and illustrate pitfalls, including "Haufen-look-alikes," which can hamper the interpretation. Urin... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981900 Thu, 01 Oct 2009 00:00:00 +0100 2981900 http://www.medworm.com/index.php?rid=2981899&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895296%26dopt%3DAbstract Authors: Ru YX, Mao BY, Zhang FK, Pang TX, Zhao SX, Liu JH, Wickramasinghe SN Severe malarial anemia causes considerable mortality and morbidity in endemic areas. Possible mechanisms underlying the anemia include lysis of parasitized and nonparasitized red cells as well as parasite product-mediated effects on erythropoiesis. The latter include suppression of erythropoiesis, dyserythropoiesis, and ineffective erythropoiesis. Present transmission electron microscope data in two cases of Pasmodium vivax malaria show a hitherto undescribed mechanism contributing to malarial anemia, namely, infection of erythroblasts by parasites and their subsequent degradation. No parasites were detected in the peripheral blood but parasites were found in the bone marrow. These findings emphasise the valu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981899 Thu, 01 Oct 2009 00:00:00 +0100 2981899 http://www.medworm.com/index.php?rid=2981898&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19895297%26dopt%3DAbstract Authors: Cangiotti AM, Morroni M Ribosome-lamella complexes (RLCs) are mainly observed in a variety of hematological disorders and occasionally in solid neoplasms and in nonneoplastic diseases. These intracytoplasmic organelles are held to arise from rough endoplasmic reticulum, but, in agreement with more recent literature data, their function is still unclear. Ultrastructural analysis of glomeruli from a patient with focal segmental glomerulosclerosis secondary to metabolic syndrome disclosed significant foot process loss and abundant cytoskeletal proteins in major podocyte processes; two of the latter also displayed RLCs. This is the second report of RLCs in human renal glomerulus. Their close association with cytoskeletal proteins and lysosomes suggests a relationship with abnormal... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2981898 Thu, 01 Oct 2009 00:00:00 +0100 2981898 http://www.medworm.com/index.php?rid=2765632&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728229%26dopt%3DAbstract This study uses gene expression profiling by quantitative real time polymerase chain reaction (qRT-PCR) to identify "intrinsic" subtypes in a Saudi population of breast cancers and compares the distribution of subtypes to the more commonly profiled Caucasian population. In addition, the immunohistochemical profile of breast cancers was correlated to the gene expression analysis. Discrepancy rate of 39% in subtype prediction between gene expression and immunohistochemical profile of the tumors was noticed. Most of this variation was in the luminal subtype. Frequency of HER2+ subtype in the Saudi cases was high (28%) by both the immunohistochemistry (IHC) and the qRT-PCR classification. Triple-negative tumors comprised 39% while only 11% showed a basal-like profile. Analysis of larger cohort... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765632 Tue, 30 Jun 2009 23:00:00 +0100 2765632 http://www.medworm.com/index.php?rid=2765631&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728230%26dopt%3DAbstract Authors: Magalhães RF, Urso Pitassi LH, Lania BG, Barjas-Castro ML, Neves Ferreira Velho PE The authors present the case of a young man with aplastic anemia who went into shock and died after several red blood cell unit transfusions. Immunohematological studies did not show any abnormality and blood cultures from patients and blood bags were negative. The ultrastructural findings, allied with current scientific knowledge, permitted the diagnosis of Bartonella sp. infection. In face of this diagnosis, two possibilities should be considered: the first one is that the patient was already infected by the bacteria before the last RBC unit transfusion. The pathogen could be involved in aplastic anemia etiology and in the failure to recover hemoglobin levels, in spite of the transfusions... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765631 Tue, 30 Jun 2009 23:00:00 +0100 2765631 http://www.medworm.com/index.php?rid=2765630&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728231%26dopt%3DAbstract In conclusion, this study reports the fine definition of pulse granuloma at microscopic level by CLSM and the results warrant the use of this technique for further analyses. PMID: 19728231 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765630 Tue, 30 Jun 2009 23:00:00 +0100 2765630 http://www.medworm.com/index.php?rid=2765629&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728232%26dopt%3DAbstract Authors: Liu Y, Geng L, Suo Z The human pro-monocytic leukemia U937 cell line was previously reported to become rho degrees cells after a long-term ethidium bromide exposure. In the authors' extensive PCR studies with different pairs of primers for the mtDNA molecule they showed that these U937-rho degrees cells, after being cultured in their laboratory for a time, did replete their mtDNA. That the cells grew well in the normal medium (RPMI 1640 plus 10% fetal calf serum and 2 mM l-glutamine) as the parental cells also suggests that these cells contain functional mitochondria and mtDNA molecules. Further experiments showed that the cells cultured in the medium with pyruvate and uridine rather rapidly and strongly adhered on the culture flask walls while the cells cultured in the medium... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765629 Tue, 30 Jun 2009 23:00:00 +0100 2765629 http://www.medworm.com/index.php?rid=2765628&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728233%26dopt%3DAbstract Authors: Rampisela D, Donner LR The authors report the first case of perineurioma of the adrenal gland. The tumor was composed of elongated wavy spindle cells focally arranged in a fascicular pattern. It was positive for epithelial membrane antigen (EMA) and claudin-1, and was negative for S-100 protein and glial fibrillary acidic protein (GFAP). Electron microscopy showed long, slender cytoplasmic processes coated by discontinuos basal lamina and presence of many pinocytotic vesicles. PMID: 19728233 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765628 Tue, 30 Jun 2009 23:00:00 +0100 2765628 http://www.medworm.com/index.php?rid=2765627&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728234%26dopt%3DAbstract Authors: Guimaraes Fde S, de Oliveira SM, de Oliveira CC, Donatti L, Buchi Dde F The performance of a moderately shorter fixation protocol for transmission electron microscopy (TEM) was evaluated by analyzing the cell structure quality after the processing. The relevance of this experimental technique is mainly based on reducting time of the steps of conventional protocols: fixation, washes, dehydration, and epoxy resin infiltration. Two sources of murine cells were used, the peritoneal and mesenteric lymph node cells. A fixation and material processing faster than usual methods can save time and improve results. Samples analysis indicated good preservation of different cell structures and organelles after this protocol. PMID: 19728234 [PubMed - in process] (Source: Ultrastructural... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765627 Tue, 30 Jun 2009 23:00:00 +0100 2765627 http://www.medworm.com/index.php?rid=2765626&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728235%26dopt%3DAbstract In this study, the authors aimed to disclose whether the hoechst33342 staining required extensive optimization for identifying SP cells in the human ovarian cancer cell line OVCAR-3. After systematic evaluations, it was found that only 2.5 microg/mL hoechst33342 staining of the cells for 60 min could get an ideal SP population, which accounted for 0.9% of the whole cell population. The sorted SP cells showed significantly higher colony formation efficiency than the non-side population (NSP) cells, and only the SP cells could form holoclones. Real-time PCR disclosed that SP cells expressed higher levels of "stemness" gene Oct3/4 than the NSP cells did, indicating that the SP cells might harbor cancer stem cells in this cell line. The results highlight the necessity of SP method optimization... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2765626 Tue, 30 Jun 2009 23:00:00 +0100 2765626 http://www.medworm.com/index.php?rid=2538903&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479649%26dopt%3DAbstract Authors: Potgieter M, Pretorius E, Oberholzer HM The numerous protective effects of coenzyme Q10 (CoQ10) evoked the question of whether it might be able to elicit protection to cell membranes after being challenged by the membrane disrupter Triton X-100. Cardiac and skeletal muscle tissue from chick embryos was cultured and exposed to increasing concentrations of CoQ10 and Triton X-100. Scanning electron microscopy was used to study cell morphology. Results suggested the ability of CoQ10 to offer protection to cells challenged by Triton X-100. The authors suggest that CoQ10 may offer protection to muscle cells, by enhancing membrane repair via patch formation by an unknown mechanism that possibly involves Ca(2+)-dependent ion channel activation. PMID: 19479649 [PubMed - in process]... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538903 Sat, 27 Jun 2009 21:36:02 +0100 2538903 http://www.medworm.com/index.php?rid=2538902&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479650%26dopt%3DAbstract Authors: Castejon OJ In a vascular anomaly showing moderate edema, the extracellular space appeared apparently normal, exhibiting a membrane to membrane space of about 20 nm in width. In congenital hydrocephalus, this space appeared notably enlarged and occupied by an electron transparent, nonproteinaceous interstitial edema fluid, due to abnormal accumulation of cerebrospinal fluid. In brain trauma, the distended extracellular space contained either electron-lucid nonproteinaceous or electron-dense proteinaceous edema fluid. Hemorrhagic foci, fibrinoid material, and non-nervous invading cells, such as macrophages and monocytes, were also found. In brain tumors, the widened extracellular space showed electron-dense proteinaceous edema fluid and bundles of fibrinoid material. The enlarg... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538902 Sat, 27 Jun 2009 21:36:02 +0100 2538902 http://www.medworm.com/index.php?rid=2538901&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479651%26dopt%3DAbstract Authors: Bin-Jaliah I, Dallak M, Haffor AS Hyperoxia (HP) exposure inducts reactive oxygen species (ROS) in the lungs that may result in lung injury, including alveolar epithelial and endothelial cells. Lactate dehydrogenase (LDH) activity relates to glycolysis, whereas glutathione peroxidase (Gpx) activity relies on the pentose phosphate pathway (PPP). The purpose of this study was to examine early ROS-induced alveolar pathological changes in relation to the activity of glutathione peroxidase (GPx) and lactate dehydrogenase (LDH) activity. Twenty adult male rats, matched with age and body weight, were randomly assigned to two groups, control and experimental. The experimental group was exposed to hyperoxia for 24 h. Ultrastructure examination showed degenerated pneumocyte type I, cont... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538901 Sat, 27 Jun 2009 21:36:02 +0100 2538901 http://www.medworm.com/index.php?rid=2538900&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479652%26dopt%3DAbstract Authors: Garcia CA, Abell-Aleff PC, Gamb SI, Miller DV Amyloidomas are localized mass-forming deposits of amyloid that occur with or without association with systemic amyloidosis. The ultrastructural findings in 3 amyloidomas from 2 autopsy patients with primary systemic AL amyloidosis are described. By transmission electron microscopy, there were randomly oriented nonbranching fibrils showing some unusual curvilinear forms and considerable variability in fibril diameter (two subsets of fibrils, one 12-14 nm and another 28-30 nm in diameter). The larger fibrils showed features of microtubule formation. Scanning electron microscopy demonstrated complex 3-dimensional tangles of fibrils. These findings add to the current ultrastructural and morphologic spectrum of paraprotein deposition d... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538900 Sat, 27 Jun 2009 21:36:02 +0100 2538900 http://www.medworm.com/index.php?rid=2538899&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479653%26dopt%3DAbstract Authors: Sipponen A, Peltola R, Jokinen JJ, Laitinen K, Lohi J, Rautio M, Mannisto M, Sipponen P, Lounatmaa K Resin salve prepared from Norway spruce (Picea abies) has been used for centuries in traditional medicine to treat skin diseases. The authors studied with transmission and scanning electron microscopy, and with electron physiology, changes in cell wall and cell membrane of Staphylococcus aureus after exposure of the bacterial cultures to resin. After exposure, cell wall thickening, cell aggregation, changed branching of fatty acids, and dissipation of membrane potential of the bacterial cells were observed. The authors conclude that spruce resin affects the cell viability via changes in the cell wall and membrane, and impairs, thereby, the synthesis of energy in the bacteria. ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538899 Sat, 27 Jun 2009 21:36:02 +0100 2538899 http://www.medworm.com/index.php?rid=2538898&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19479654%26dopt%3DAbstract Authors: Ru YX, Eyden B, Li QH, Mao BY, Liu EB, Pang TX Primary and metastatic carcinomas have a reactive stroma characterized by many myofibroblasts. These cells have also been documented in nonepithelial malignancies, such as sarcomas, malignant melanoma, and lymphoid tumors but in generally far fewer numbers. In non-Hodgkin's lymphoma, Hodgkin's disease, and leukemia, myofibroblasts are rather rarely documented. In particular, there appear to be no reports of myofibroblasts in either primary bone-marrow/peripheral blood leukemia or secondary deposits of leukemia. In this paper, a case of a relapsed chronic myeloid leukemia appearing in an inguinal lymph node is described, containing many myofibroblasts. The case is detailed and presented with a discussion on the role of myofibroblas... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2538898 Sat, 27 Jun 2009 21:36:02 +0100 2538898 http://www.medworm.com/index.php?rid=2253505&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274579%26dopt%3DAbstract Authors: Al-Johany AM, Haffor AS Animal studies on the toxicity of heavy metals have been widely used as model to simulate the impacts of environmental pollution on the human health. In the present study the authors hypothesized that cadmium exposure inducts changes in reactive oxygen species (ROS) and that may be involved in the pathogenesis of lung diseases. The pathological changes of different pulmonary cells of ROS-cadmium-dependent effects were investigated in relation to the activity of lactate dehydrogenase (LDH) and glutathione peroxidase (GPx). Twelve animals were randomly assigned to two groups, control and experimental. The experimental group underwent ingestion of cadmium mixed with diet (200 mg/kg) for 7 weeks. Following the treatment conditions for each group, blood samp... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253505 Sun, 01 Mar 2009 05:00:00 +0100 2253505 http://www.medworm.com/index.php?rid=2253504&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274580%26dopt%3DAbstract Authors: Scivetti M, Lucchese A, Crincoli V, Pilolli GP, Favia G Liesegang rings are concentric noncellular lamellar structures, occasionally found in inflammatory tissues. They have been confused with various parasites, algas, calcification, and psammoma bodies. The authors examined Liesegang rings from oral inflammatory cysts by both optical and confocal laser scanning microscopy, and perfomed a three-dimensional reconstruction. These investigations indicate that Liesegang rings are composed of multiple birefringent concentric rings, resulting from a progressive deposition of organic substances, with an unclear pathogenesis. PMID: 19274580 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253504 Sun, 01 Mar 2009 05:00:00 +0100 2253504 http://www.medworm.com/index.php?rid=2253503&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274581%26dopt%3DAbstract Authors: Kang D, Kunugi S, Masuda Y, Ishizaki M, Koizumi K, Fukuda Y Three cases of biphasic mesothelioma and 2 cases of sarcomatoid mesothelioma were investigated using light and electron microscopy. In 2 of the 3 cases of biphasic mesotheliomas, fibrous long-spacing (FLS) collagen fibrils were discovered with a symmetrical cross-striation of 130 nm in periodicity. However, no connection between the FLS fibrils and usual collagen fibrils were observed. Periodic acid silver methenamine stain revealed unstained bands with periods of 130 nm in FLS fibrils, whereas the usual collagen fibrils showed continuous positive staining. All 3 cases of biphasic mesotheliomas showed deposits of hyaluronic acid, whereas both cases of sarcomatoid mesotheliomas showed little hyaluronic acid. As a high ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253503 Sun, 01 Mar 2009 05:00:00 +0100 2253503 http://www.medworm.com/index.php?rid=2253502&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274582%26dopt%3DAbstract Authors: Eyden B, Wang G, Yao L Epithelioid sarcoma is an uncommon but well-described malignancy, which is found predominantly in the soft tissues of the young and middle-aged, and which pursues an indolent to aggressive course. It shows a degree of both mesenchymal and epithelial differentiation. Myofibroblastic differentiation has been recorded in epithelioid sarcoma for some time, the evidence being based mainly on the presence of smooth-muscle-type myofilaments and, more recently, on alpha-smooth-muscle actin and muscle-specific actin immunostaining. Myofibroblastic differentiation based on the stricter criterion of the fibronectin fibril/fibronexus junction has not so far been demonstrated except for a single atypical case with spindle-cell morphology and a cytokeratin-negative im... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253502 Sun, 01 Mar 2009 05:00:00 +0100 2253502 http://www.medworm.com/index.php?rid=2253501&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274583%26dopt%3DAbstract The objective of this paper is to produce an ultrastructural classification of acute monocytic leukemia (AML-M5) in relation to clinical behaviors. The ultrastructural characteristics of blasts of the monocytic series were analyzed in 72 M5 patients by transmission electron microscopy (TEM), in terms of their content of typical monoblasts, atypical monoblasts, atypical promonocytes, and typical promonocytes in bone-marrow aspirates. Four kinds of monocytic blasts were identified by cell size and shape, nuclear profile, nucleocytoplasmic ratio, heterochromatin content, nucleolus, granules, vesicles, and Golgi apparatus. Their characteristics of remission rate, cytochemistry, immunophenotype, and cytogenetics were also investigated. The data obtained permitted M5 patients to be divided into ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253501 Sun, 01 Mar 2009 05:00:00 +0100 2253501 http://www.medworm.com/index.php?rid=2253500&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274584%26dopt%3DAbstract Authors: Walpusk J, Galambos C Despite the widespread utilization of immunohistochemical stains in pathologic practice, virus identification remains a challenge. In our institution, electron microscopy combined with the lift technique has been utilized for 40 years as a reliable diagnostic tool where a question of viral infection is raised by light microscopic observation and could not be otherwise confirmed. The combination of light microscopic and ultrastructural methods has allowed us to examine individual cells suspicious for harboring viral particles previously identified on a hematoxylin-eosin stained tissue section. In this review we describe the lift technique in detail with our modifications in the hope that the use of lift technique in these uncommon but specific circumstance... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253500 Sun, 01 Mar 2009 05:00:00 +0100 2253500 http://www.medworm.com/index.php?rid=2253498&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19274585%26dopt%3DAbstract Authors: Toumpanaki A, Baltatzis GE, Gaitanarou E, Seretis E, Toumpanakis C, Aroni K, Kittas C, Voloudakis-Baltatzis IE The aim of the present study was to observe possible qualitative and quantitative expression differences between nuclear matrix proteins (NMPs) of human colon adenocarcinoma and their mirror biopsies, using the technique of two-dimensional gel electrophoresis, in order to identify the existence of specific NMP fingerprints for colon cancer. Colon tissues were examined ultrastructurally and NMPs were isolated biochemically, by serial extraction of lipids, soluble proteins, DNA, RNA, and intermediate filaments and were separated according to their isoelectric point (pI) and their molecular weight (MW) by high-resolution two-dimensional electrophoresis (2D). By comparing... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2253498 Sun, 01 Mar 2009 05:00:00 +0100 2253498 http://www.medworm.com/index.php?rid=2162637&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191195%26dopt%3DAbstract In this study, 4 cases of human gastric carcinomas with massive neutrophil infiltration were studied by light and electron microscopy, focusing on the inflammatory angiogenesis in the tumor stroma. At light microscopy, the tumors were advanced gastric carcinomas in which various degrees of tubular differentiation were present. Under an electron microscope, pericytes exhibited two major differentiated states with distinct ultrastructural features: a contractile phenotype and a synthetic phenotype. The contractile phenotype was characterized by abundant microfilaments. Synthetic pericytes contained abundant rough endoplasmic reticulum, lipid bodies, and numerous membrane-bound vesicles. These ultrastructural findings extend concept of contractile/synthetic phenotype modulation, originally de... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162637 Thu, 01 Jan 2009 05:00:00 +0100 2162637 http://www.medworm.com/index.php?rid=2162636&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191196%26dopt%3DAbstract Authors: Kaarteenaho-Wiik R, Paakko P, Sormunen R Fibroblast differentiation into myofibroblast in transforming growth factor-beta1-exposed human lung fibroblasts and the immunolocalizations of alpha-smooth muscle actin, fibronectin, tenascin-C, and osteopontin in exposed cells were studied by conventional transmission electron microscopy and immunoelectron microscopy. Ultrastructural features of myofibroblasts were detected after exposure, e.g., alpha-smooth muscle actin positive bundles in the cytoplasm of cells and extracellular fibronectin-containing structures on the surface of the cell forming fibronexus structure, osteopontin adjacent to rough endoplastic reticulum and extracellular tenascin-C in the vicinity of the cell. The authors concluded that exposure to transforming growt... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162636 Thu, 01 Jan 2009 05:00:00 +0100 2162636 http://www.medworm.com/index.php?rid=2162635&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191197%26dopt%3DAbstract Authors: Eyden B Basal/external lamina is not found over plasma cells or other hemolymphoid cells, and the feature can have diagnostic value in distinguishing the neoplastic counterparts of such cells from epithelium, endothelium, mesothelium, and so on, which do have this feature. In this paper, a material ultrastructurally indistinguishable from basal or external lamina is reported on reactive plasma cells found in a fibrous pseudotumor, intralobular stroma of normal breast, tumor stroma of squamous cell carcinoma, and submucosa of normal human small intestine. It was focal, followed the contours of the cell-surface membrane, was lightly textured, 40-80 nm thick, and separated from the plasma cell surface membrane by a clear space resembling a lamina lucida. Its function remains unce... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162635 Thu, 01 Jan 2009 05:00:00 +0100 2162635 http://www.medworm.com/index.php?rid=2162634&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191198%26dopt%3DAbstract Authors: Mansy SS, Abdelfatah AS, Hassanein MH The present work highlights intracellular viral morphogenesis and virus-host cell interactions in patients proved to be infected with HCV. The material of this study consisted of 28 liver biopsies taken from patients positive for serum HCV-RNA by polymerase chain reaction. Liver biopsies were processed for light and electron microscopic examination. Ultrastructural findings of this work supported a new hypothesis for the turnover of HCV to retrovirus and described the presumed involved mechanism. This novel perception offers important insights that can explain the vague mechanisms of HCV behavior in the infected hepatocytes. PMID: 19191198 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162634 Thu, 01 Jan 2009 05:00:00 +0100 2162634 http://www.medworm.com/index.php?rid=2162633&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191199%26dopt%3DAbstract Authors: Eyden B The fibronexus is recognized as a characteristic marker of the myofibroblast. However, it is not completely specific for this cell, having been seen in aortic smooth muscle (in attenuated form) and endothelium in experimental animals. This paper documents fibronexus junctions in human in vivo endothelium. Ultrastructural observations were made on the vasculature of a desmoplastic and focally neurotropic malignant melanoma. Cross-sectioned fibronectin fibrils were seen outside the stromal surface of the endothelial plasmalemma. Often, they were positioned directly opposite the actin-filament bundles in the peripheral cytoplasm. Neoplastic and in vitro cultured cells apart, endothelium is the only nonmyofibroblastic cell type to show well-developed fibronexus junctions. ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162633 Thu, 01 Jan 2009 05:00:00 +0100 2162633 http://www.medworm.com/index.php?rid=2162632&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19191200%26dopt%3DAbstract Authors: Eyden B, Chakrabarty B, Hatimy U Lymphoma diagnosis rarely needs electron microscopy (EM), but one area where it can be useful is in the distinction of cytokeratin-positive lymphoma from carcinoma. The authors describe such a case, where difficulties were encountered due to lack of antibody specificity, distinguishing reactive from tumoral cells, and suboptimal sampling for EM. The tumor was in a lymph node next to the right submandibular gland in a 69-year-old man. This was a malignant tumor, composed of sheets of monomorphic large round cells. Interpretation on the part of a team of pathologists who examined this tumor was divided. On histological sections, the differential diagnosis was between carcinoma and lymphoma, which was modified to cytokeratin-positive lymphoma vers... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2162632 Thu, 01 Jan 2009 05:00:00 +0100 2162632 http://www.medworm.com/index.php?rid=2071892&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117263%26dopt%3DAbstract Authors: Zouvani I, Aristodemou S, Hadjisavvas A, Michael T, Vassiliou M, Patsias C, Pierides A, Ioannou K, Kyriacou K Thin basement membrane nephropathy is one of the main causes of hematuria in both children and adults. It is often associated with a family history and its true incidence is unknown. Accurate diagnosis of thin basement membrane nephropathy relies on the presence of attenuated glomerular basement membranes, a finding that can be appreciated only by examination in the electron microscope. In Cyprus the department of electron microscopy has received 990 consecutive renal biopsies for diagnosis. The aim of this study is to define the incidence of thin basement membrane nephropathy in this population sample based on the results of electron microscopy. PMID: 19117263 [Pu... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071892 Sat, 01 Nov 2008 04:00:00 +0100 2071892 http://www.medworm.com/index.php?rid=2071891&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117264%26dopt%3DAbstract Authors: Davidson B Malignant mesothelioma is a primary cancer of the serosal cavities, an anatomic site that is also frequently affected by metastatic disease, predominantly from primary carcinomas of the lung, breast, and ovary. Advances in immunohistochemistry have resulted in improved diagnostic sensitivity and specificity in the differential diagnosis between metastatic adenocarcinoma and malignant mesothelioma in both cytological and histological material. Recently, the author's group applied high throughput technology to the identification of new markers that may aid in differentiating malignant mesothelioma from ovarian and peritoneal serous carcinoma, tumors with closely related histogenesis and antigenic profile. In addition to the improved tools available for serosal cancer ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071891 Sat, 01 Nov 2008 04:00:00 +0100 2071891 http://www.medworm.com/index.php?rid=2071890&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117265%26dopt%3DAbstract In conclusion, their ultrastructural features showed that GSPCs were at the primary stage of differentiation, and could even partially reveal the underlying reasons for the malignant proliferation and differential inhibition of GSPCs. PMID: 19117265 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071890 Sat, 01 Nov 2008 04:00:00 +0100 2071890 http://www.medworm.com/index.php?rid=2071889&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117266%26dopt%3DAbstract Authors: Finzi G, Franzi F, Placidi C, Acquati F, Palumbo E, Russo A, Taramelli R, Sessa F, La Rosa S BACE2 is a protease homologous to BACE1 protein, an enzyme involved in the amyloid formation of Alzheimer disease (AD). However, despite the high homology between these two proteins, the biological role of BACE2 is still controversial, even though a few studies have suggested a pathogenetic role in sporadic inclusion-body myositis and hereditary inclusion-body myopathy, which are characterized by vacuolization of muscular fibers with intracellular deposits of proteins similar to those found in the brain of AD patients. Although BACE2 has also been identified in the pancreas, its function remains unknown and its specific localization in different pancreatic cell types has not been defin... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071889 Sat, 01 Nov 2008 04:00:00 +0100 2071889 http://www.medworm.com/index.php?rid=2071888&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19117267%26dopt%3DAbstract Authors: Wartchow EP, Trost BA, Tucker JA, Albano EA, Mierau GW Renal medullary carcinoma is a recently described highly aggressive malignancy that in most instances exhibits a constellation of clinical and light microscopic features sufficiently distinctive to enable a quick and confident diagnosis. Presented are three examples where, because of unusual elements in the clinical presentation, electron microscopic examination proved beneficial in establishing the diagnosis. PMID: 19117267 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=2071888 Sat, 01 Nov 2008 04:00:00 +0100 2071888 http://www.medworm.com/index.php?rid=1919809&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958788%26dopt%3DAbstract This study reports changes in the frequency of detection of various asbestos fiber types between 1982 and 2005. Crocidolite is increasingly detected in U.S. mesothelioma patients. The percentage of crocidolite fibers detected in lung tissue has risen from 4 to 10%, and the percentage of cases in which crocidolite was detected increased from 19 to 37%. Meanwhile, the frequency of detection of amosite and chrysotile has decreased. The authors performed a detailed analysis of cases in which crocidolite was identified in the absence of amosite. Most of such cases were identified in recent years, a finding of concern since crocidolite is considered the most potent fiber type with respect to the pathogenesis of mesothelioma. PMID: 18958788 [PubMed - in process] (Source: Ultrastructural Patho... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919809 Mon, 01 Sep 2008 04:00:00 +0100 1919809 http://www.medworm.com/index.php?rid=1919808&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958789%26dopt%3DAbstract Authors: Lepreux S, Doudnikoff E, Aubert I, Bioulac-Sage P, Bloch B, Martin-Negrier ML Human telomerase comprises a catalytic protein subunit (hTERT) and an RNA subunit (hTR). Telomerase extends chromosome ends in compensation for the attrition of the telomeres during replication. In this work, the authors explore the expression of hTERT and hTR in neutrophils, respectively by immunochemistry techniques and in situ hybridization. hTERT was strongly expressed in neutrophils cytoplasm. The ultrastructural study showed that the gold particles were not associated with specific organelles but scattered in the cytosol. hTR was not expressed. hTERT is expressed in the cytoplasm of neutrophils, but its roles-eventually extratelomeric effects-remain to be elucidated. PMID: 18958789 [PubMed ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919808 Mon, 01 Sep 2008 04:00:00 +0100 1919808 http://www.medworm.com/index.php?rid=1919807&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958790%26dopt%3DAbstract Authors: Caruso RA, Fedele F, Finocchiaro G, Pizzi G, Nunnari M, Gitto G, Fabiano V, Rigoli L Ultrastructural findings in three cases of gastric carcinoma with coagulative necrosis are reviewed with special emphasis on microvascular changes. Intratumoral microvasculature revealed more or less stabilized vessels. Some were characterized by a close association between pericytes and endothelial cells, whereas others showed laminated basement membrane, with a loose association between pericytes and endothelial cells. Some mural cells exhibited ultrastructural signs of regressive changes, including lipofuscin granules, swollen mitochondria, and cytoplasmic lucency. These findings are discussed in relationship to a number of recent studies of the microvascular injury caused by hypoxia and re... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919807 Mon, 01 Sep 2008 04:00:00 +0100 1919807 http://www.medworm.com/index.php?rid=1919806&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958791%26dopt%3DAbstract Authors: Pilolli GP, Lucchese A, Maiorano E, Favia G To define the value of confocal laser scanning microscopy (CLSM) as a practical method for a qualitative and quantitative analysis of hard tissue, the authors have analyzed normal maxillo-facial bone. They obtained and analyzed 58 bone samples from 28 patients who underwent to implant surgery. All the samples presented intense autofluorescence primarily ascribed to collagen. Variable degrees of autofluorescence have been identified between osteones and interosteonic bone. CLSM allowed improved tissue imaging, bidimensional pictures with better resolution at cellular level, and, in particular, the possibility of different histomorphometric evaluation. The application of CLSM to bone histomorphometry represents a new and never describe... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919806 Mon, 01 Sep 2008 04:00:00 +0100 1919806 http://www.medworm.com/index.php?rid=1919805&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958792%26dopt%3DAbstract Authors: Chen R, Chen JY, Zhou LW Although the spatial and temporal distributions of cellular NAD(P)H concentrations have been theoretically predicted as typical patterns of the metabolism in living cells, so far such a pattern was observed only in neutrophils. In this work, the dynamic NAD(P)H distributions in rat basophilic leukemia (RBL-2H3) and human hepatocellular carcinoma (Hep G2) cells were studied by imaging the autofluorescence of cellular NAD(P)H with a sensitive CCD detector in a confocal microscope. The typical pattern of the cytoplasmic NAD(P)H wave traveling along the long axis of the elongated cell with a velocity of 2.2+/-0.6 mircom/s was detected in RBL-2H3 cells. While in the case of Hep G2 cells, only the oscillation of the mitochondrial NAD(P)H was observed because... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919805 Mon, 01 Sep 2008 04:00:00 +0100 1919805 http://www.medworm.com/index.php?rid=1919804&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958793%26dopt%3DAbstract Authors: Vered M, Polak-Charcon S, Babushkin T, Dayan D The 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinoma, in which the carcinogen is administered systemically in drinking water, is the most comparable animal model to the development of human oral carcinoma. This is the first study to report the ultrastructural changes in this model. The most significant changes were observed in the carcinoma cells at the invasion front and included unique modifications in the basal lamina, presence of micropinocytotic vesicles (plasmalemmal caveolae), and emergence of cytoplasmic microfilaments featuring a parallel arrangement. The microfilaments, in both appearance and organization, were consistent with contractile microfilaments. These observations may be the morphological reflection ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919804 Mon, 01 Sep 2008 04:00:00 +0100 1919804 http://www.medworm.com/index.php?rid=1919803&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958794%26dopt%3DAbstract Authors: Donato G, Conforti F, Zuccala V, Cosco C, Laratta C, Perrotta I, Tripepi S, Amorosi A Ultrastructural data about large cell variant ovarian small cell carcinoma (LCV-SCC) are scarce and contradictory and the role of transmission electronmicroscopy (TEM) is not clear in the assessment of such tumors. The authors present a case of LCV-SCC without hypercalcemia in a 30-year-old woman. The diagnosis was confirmed by histopathological and immunohistochemical studies. Cytopathological examination of peritoneal washing showed a population of large neoplastic cells. TEM demonstrated that the neoplasia comprised two types of cells: one type showed many coarse secretory granules without dense core, and the other type was without granules and showed dilated endoplasmic reticulum and some... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919803 Mon, 01 Sep 2008 04:00:00 +0100 1919803 http://www.medworm.com/index.php?rid=1919802&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18958795%26dopt%3DAbstract This study revealed a spectrum of endothelial cell ultrastructure from lymphatic to blood vascular. It demonstrated frequent Weibel-Palade bodies and gap junctions. The spindle cells were shown to behave as facultative phagocytes, internalizing and processing necrotic cells and leaked red blood cells (RBCs). Fragmented RBCs were equivalent to the "hyaline droplets" seen by light microscopy. The final stages of RBC disintegration were hemosiderin and ferritin. Most significantly, this study disclosed that KS is actually composed of a single type of randomly oriented spindle cell forming vessels of varying size and integrity. PMID: 18958795 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1919802 Mon, 01 Sep 2008 04:00:00 +0100 1919802 http://www.medworm.com/index.php?rid=1762205&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696397%26dopt%3DAbstract Authors: Bronner IM, Hoogendijk JE, Veldman H, Ramkema M, van den Bergh Weerman MA, Rozemuller AJ, de Visser M In dermatomyositis (DM) there is strong histopathological evidence of a microvascular pathogenesis, including endothelial microtubular inclusions. In nonspecific myositis, perimysial and perivascular infiltrates in the muscle biopsy similar to DM are found. Microtubular inclusions in endothelial cells were systematically searched for and found in 4 of the 20 muscle biopsies of nonspecific myositis patients (20%). Three had a CTD (SLE, scleroderma, and Sjogren syndrome). Ten patients with DM and 5 patients with sporadic inclusion body myositis served as positive and negative controls, respectively. PMID: 18696397 [PubMed - in process] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762205 Tue, 01 Jul 2008 04:00:00 +0100 1762205 http://www.medworm.com/index.php?rid=1762204&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696398%26dopt%3DAbstract Authors: Terzakis JA, Taskin M The utility of scanning electron microscopy in the evaluation of ordinary glass peripheral blood smears of patients with myelodysplasia and those uncertain for myelodysplasia is emphasized. Attention is directed to changes in segmented granulocytes. Comparison of ultrastructural findings in abnormal blood smears with control cases is made. Important findings include reduced cytoplasmic granule number, increased cell size, large cytoplasmic vacuoles, condensation of the peripheral cytoplasm, prominence of large cytoplasmic granules, irregular cytoplasmic perimeter, abnormal nuclear morphology, abnormal cell shape, and a necklace-like arrangement of cytoplasmic granules. Of these findings, reduced cytoplasmic granule number was the most specific finding, wh... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762204 Tue, 01 Jul 2008 04:00:00 +0100 1762204 http://www.medworm.com/index.php?rid=1762203&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696399%26dopt%3DAbstract This study was conducted on 61 patients (27 males and 34 females). The age ranged from 27 to 81 years (mean 54 years). All suffered from oral squamous cell carcinoma and were treated by surgery and deep X-ray therapy (DXT). The UICC and TNM classification and staging recommendations were used for evaluation of the patients. All sections were stained with Ag-NORs stain for examination of the proliferative activity of the squamous cell carcinomas. Biopsies were also taken from another 6 cases--3 cases with normal striated muscle and 3 cases from normal oral mucosa--and served as controls. Statistical studies of Ag-NOR scores were classified into 3 scores: the p values of score I (ANOVA test) were .0001, score II (ANOVA test) was .0001, and score III (ANOVA test) was 06. Both scores I and II ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762203 Tue, 01 Jul 2008 04:00:00 +0100 1762203 http://www.medworm.com/index.php?rid=1762202&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696400%26dopt%3DAbstract Authors: Smit E, Pretorius E, Anderson R, Oommen J, Potjo M Canova is an immunomodulatory, homeopathic preparation that has been shown to activate macrophages in vitro and in vivo, with resultant enhanced spreading of the cells and formation of microvillus extensions from the cell body. Since monocytes are the precursor cells of macrophages and dendritic cells, the objective of the current study was to investigate the effects of Canova on the differentiation of human blood monocytes in vitro. Monocytes were isolated, grown in culture, and exposed to 10 and 20% Canova without the addition of cytokines. After 48 h, monocytes were prepared for analysis by scanning electron microscopy, while cells kept in culture for 7 days and exposed to Canova on days 1, 3, and 4 were analyzed by flow cy... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762202 Tue, 01 Jul 2008 04:00:00 +0100 1762202 http://www.medworm.com/index.php?rid=1762201&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696401%26dopt%3DAbstract Authors: Caruso RA, Cicciarello R, Gagliardi ME, Albiero F, Costa G, Fedele F, Cavaliere R, Finocchiaro G, Mesiti M, Cavallari V A primary invasive micropapillary carcinoma of the breast in a 46-year-old woman is reported. Histologically, it was composed predominantly of papillary tumor cell clusters without fibrovascular cores, surrounded by a clear space. Tumor cells were positive for cytokeratin (CK) 7, estrogen receptor (ER), and progesterone receptor (PR), but negative for p53, CK 20, CD34, c-Erb-B2, CK5, epidermal growth factor receptor (EGFR), vimentin, and c-kit. MUC1 expression was found at the reversed apical membrane of neoplastic cell clusters. Accordingly, electron microscopy showed the lack of basement membrane and presence of microvilli at the basal surface of the tumor ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762201 Tue, 01 Jul 2008 04:00:00 +0100 1762201 http://www.medworm.com/index.php?rid=1762200&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18696402%26dopt%3DAbstract Authors: Orenstein JM The purpose of this study was to characterize the ultrastructure of lymphoid tissue from HIV/AIDS patients and to evaluate it as a reservoir and source of HIV. HIV has been demonstrated in lymph nodes and tonsils and adenoids, by immunohistochemistry (IHC), in situ hybridization (ISH), and transmission electron microscopy (TEM), to be associated with germinal center (GC) follicular dendritic cells (FDC). The presence of HIV in the larger gastrointestinal tract-associated lymphoid tissue (GALT) has been much less studied. Whether FDC themselves are productively infected by HIV in any of the lymphoid sites is controversial. Lymph nodes, tonsils, and gastrointestinal biopsies were fixed in neutral buffered glutaraldehyde and prepared for TEM. Mature HIV particles wer... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762200 Tue, 01 Jul 2008 04:00:00 +0100 1762200 http://www.medworm.com/index.php?rid=1762212&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570151%26dopt%3DAbstract Authors: Pretorius E, Oberholzer HM, Smit E, Steyn E, Briedenhann S, Franz CR Several hematological abnormalities associated with HIV have been documented, but the mechanisms responsible for the cytopenias in AIDS patients are complex and not always completely understood. Thrombocytopenia, which occurs in about 40% of patients with HIV infection, may be caused by increased peripheral platelet destruction, a defect in platelet production due to the impaired formation of platelets by HIV-infected magakaryocytes, or a combination of these. The aim of this study was to compare the morphology of the platelet aggregates in platelet-rich plasma (PRP) clots prepared from HIV patients with those of controls without HIV. These platelet aggregates were studied using the scanning electron microsco... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762212 Thu, 01 May 2008 04:00:00 +0100 1762212 http://www.medworm.com/index.php?rid=1762211&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570152%26dopt%3DAbstract The objective of this study was to investigate the ultrastructural characteristics of nucleated cells in the bone marrow of patients with aplastic anemia (AA). This was done by observing the morphology of nucleated cells in bone marrow aspirates from 20 patients with AA by transmission electron microscopy. Erythroblasts were decreased in all cases and not observed in 6 cases. Nuclear abnormalities, such as pyknosis, karyolysis, karyorrhexis, apoptosis, and "Swiss cheese"-like changes, were found in 10 cases. Focal cytoplasmic necrotic changes and cytolysis were found in 3 cases. There were more megaloblasts in 4 cases. Abnormalities of granulocytes were found in 12 out of 18 cases. Megakaryocytes showed focal cytoplasmic necrotic changes. Most monocytes had dendritic features, including ex... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762211 Thu, 01 May 2008 04:00:00 +0100 1762211 http://www.medworm.com/index.php?rid=1762210&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570153%26dopt%3DAbstract Authors: Goscinski MA, Suo Z, Flørenes VA, Vlatkovic L, Nesland JM, Giercksky KE Fibroblast activation protein-alpha (FAP-alpha) and urokinase-type plasminogen activator (uPA) are serine proteases involved in cancer invasion and metastasis. The authors examined FAP-alpha and uPA expression in premalignant and malignant stages of esophageal adenocarcinoma by immunohistochemistry. Additionally, Western blotting was performed on fresh-frozen tissue samples. FAP-alpha and uPA were detected in metaplastic, dysplastic, and carcinoma cells, as well as in adjacent stroma. Stromal FAP-alpha expression was associated with depth of tumor invasion, while stromal uPA expression correlated with lymph node metastases in adenocarcinomas. Stromal uPA expression in cells with premalignant changes c... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762210 Thu, 01 May 2008 04:00:00 +0100 1762210 http://www.medworm.com/index.php?rid=1762209&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570154%26dopt%3DAbstract We report here a case of low-grade myofibrosarcoma with fibronexus arising in the right arm of an 80-year-old man. Histologically, the tumor was composed of relatively uniform and slender spindle cells arranged in fascicles. The nuclei with fusiform and tapered shapes were mildly hyperchromatic, but never exhibited pleomorphism. Mitotic figures were common, but no atypical mitosis was identified. At the tumor periphery, tumor cells had invaded into the surrounding skeletal muscle tissue. Tumor cells were positive diffusely for alpha-smooth muscle actin and less intensely for desmin, but were negative for h-caldesmon and S-100 protein. Ultrastructurally, tumor cells had well developed cytoplasmic organelles and varying amounts of peripheral or subplasmalemmal bundles of thin myofilaments wi... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762209 Thu, 01 May 2008 04:00:00 +0100 1762209 http://www.medworm.com/index.php?rid=1762208&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570155%26dopt%3DAbstract This study aimed at determining the subcellular localization of YKL-40, using immunogold labeling, in normal epithelial cells and in malignant tumor cells of the breast by immunoelectron microscopy. YKL-40 protein expression was redistributed in carcinoma versus normal glandular tissue of the breast. A reduced expression of YKL-40 in relation to intermediate filaments and desmosomes was found in tumor cells. Changes in YKL-40 expression suggest that the function of YKL-40 in cells of epithelial origin may be related to cell motility and cell-cell adhesion, features associated with invasion and migration potential of tumor cells. PMID: 18570155 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762208 Thu, 01 May 2008 04:00:00 +0100 1762208 http://www.medworm.com/index.php?rid=1762207&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570156%26dopt%3DAbstract Authors: Lucchese A, Pilolli GP, Petruzzi M, Crincoli V, Scivetti M, Favia G The authors used confocal laser scanning microscope to analyze human crown dentin. Specimens from 10 teeth were divided in two groups, one of which was decalcified and stained with hematoxylin and eosin. In the second group an undecalcified section was analyzed. Both groups were scanned by confocal microscope to generate optically sectioned images. All of the analyzed samples presented an intense autofluorescent that was ascribed to collagens. The degree of autofluorescence intensity was variable and might be due to collagen expression. The results indicate that a confocal microscope may be of help in analyzing and defining the nature and extent of collagen fibrils in human dentin. PMID: 18570156 [PubMed -... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762207 Thu, 01 May 2008 04:00:00 +0100 1762207 http://www.medworm.com/index.php?rid=1762206&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18570157%26dopt%3DAbstract This study suggests that FGN is mainly a renal-limited disease with possible involvement of the spleen. PMID: 18570157 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762206 Thu, 01 May 2008 04:00:00 +0100 1762206 http://www.medworm.com/index.php?rid=1762218&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446664%26dopt%3DAbstract Authors: Hicks J PMID: 18446664 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762218 Sat, 01 Mar 2008 05:00:00 +0100 1762218 http://www.medworm.com/index.php?rid=1762217&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446665%26dopt%3DAbstract Authors: Fletcher CD PMID: 18446665 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762217 Sat, 01 Mar 2008 05:00:00 +0100 1762217 http://www.medworm.com/index.php?rid=1762216&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446666%26dopt%3DAbstract Authors: Antonescu CR High-density microarrays are used to measure in a comprehensive manner large-scale ("profile") gene expression patterns (when tumor RNA is tested) or gene copy number changes (when tumor genomic DNA is tested). Microarray-based studies are increasingly useful in addressing a wide variety of questions in sarcoma biology. This review includes a brief description of the microarray methodology and data analysis, followed by few examples of the recent applications of gene expression in the diagnosis, classification, and treatment of sarcomas. PMID: 18446666 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762216 Sat, 01 Mar 2008 05:00:00 +0100 1762216 http://www.medworm.com/index.php?rid=1762215&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446667%26dopt%3DAbstract Authors: Herrera GA, Turbat-Herrera EA Due to the variable light microscopic appearance of sarcomas, a large number of neoplasms may need to be considered in the differential diagnosis in some situations. In difficult cases, the surgical pathologist must approach the differential diagnosis using ancillary diagnostic techniques and should do so in an orderly fashion, recognizing that arriving at the correct diagnosis is without a doubt the most important goal. It is also imperative to take into account the expense and time that may be involved in arriving to the definitive diagnosis, which could influence how the workup is conducted. Therefore, the most reasonable route to address the differential diagnosis in order to make a final solid diagnostic assessment should be taken. Whether im... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762215 Sat, 01 Mar 2008 05:00:00 +0100 1762215 http://www.medworm.com/index.php?rid=1762214&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446668%26dopt%3DAbstract Authors: Santucci M, Franchi A Differentiation along a distinct cell lineage can be identified either morphologically or with the adjunct of different ancillary techniques. While immunohistochemistry has progressively supplanted electron microscopy as the method of choice to characterize the phenotype of neoplastic cells in soft tissue tumors and in solving most differential diagnoses, a number of lesions still lack reliable specific markers or show overlapping antigen expression, which can be categorized by ultrastructural analysis through the recognition of submicroscopic features indicative of a specific line of differentiation. This review discusses the role of electron microscopy in the identification of the phenotype of neoplastic cells in soft tissue sarcomas, with particular re... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762214 Sat, 01 Mar 2008 05:00:00 +0100 1762214 http://www.medworm.com/index.php?rid=1762213&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18446669%26dopt%3DAbstract Authors: Bridge JA Sarcomas constitute a heterogeneous group of rare tumors that in recent years have been shown by cytogenetic analysis to have a remarkably high incidence of specific and primary alterations. These genetic alterations not only have guided molecular studies in establishing the underlying genes involved, thereby yielding important pathogenetic information, but have also provided clinicians with a valuable tool to add to their diagnostic armamentarium. The addition of molecular cytogenetic (fluorescence in situ hybridization [FISH]) and molecular approaches (reverse transcriptase-polymerase chain reaction [RT-PCR]) has further enhanced the sensitivity and accuracy of detecting nonrandom chromosomal imbalances and/or structural rearrangements in sarcomas, including assess... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762213 Sat, 01 Mar 2008 05:00:00 +0100 1762213 http://www.medworm.com/index.php?rid=1762223&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18300031%26dopt%3DAbstract Authors: Vitkov L, Kastner M, Kienberger F, Hinterdorfer P, Schilcher K, Grunert I, Dumfahrt H, Krautgartner WD Enamel bond strength is an important factor in restorative dentistry and crucially depends on the enamel roughness. To increase roughness, different etching procedures are employed and profilometric estimations, with probe profilometers, including atomic force microscopy (AFM), have been made. However, no correlation between roughness and bond strength has been found. To search for a possible error source leading to the underestimation of enamel roughness when utilizing probe profilometers, the authors compared scanning electron microscopy and AFM images of acid-etched tooth enamel. The results showed that AFM imaging cannot correctly depict the acid-etched enamel surface, be... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762223 Tue, 01 Jan 2008 05:00:00 +0100 1762223 http://www.medworm.com/index.php?rid=1762222&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18300032%26dopt%3DAbstract Authors: Liu Y, Wu H, Wu J, Wang S, Liu Y, Zhao Z, Zhang X, Li R, Guo M, Zhang Z The purpose of this report was to study the use of pre-embedding immunoelectron microscopy technique with gold and horseradish peroxidase (HRP) labeling in detecting the expression of ubiquitin C-terminal hydrolase 1 (UCH-L1) of podocytes in glomerulonephritis. The specimens of human IgA nephropathy and lupus nephritis were fixed with paraformaldehyde and lysine-HCl buffer, labeled by colloidal gold or HRP, embedded with epoxy resin, and examined under the transmission electron microscope. The high density of gold particles or peroxidase reaction products (DAB) combined with UCH-L1 was obvious in cytoplasm and processes of podocytes. This modified technique of pre-embedding immunoelectron microscopy could ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762222 Tue, 01 Jan 2008 05:00:00 +0100 1762222 http://www.medworm.com/index.php?rid=1762221&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18300033%26dopt%3DAbstract Authors: Caruso RA, Fedele F, Consolo P, Luigiano C, Venuti A, Cavallari V A rare case of pleomorphic giant cell carcinoma of the stomach in a 70-year-old man is reported. Characteristic microscopic findings included a general lack of architectural cohesiveness, aggregates of mononucleated or multinucleated giant cells, extensive areas of coagulative necrosis, and numerous mitoses. Immunohistochemically, tumor cells displayed cytoplasmic immunoreactivity for cytokeratin AE1/AE3 as well as overexpression of p53 and Ki-67. Electron microscopy revealed paranuclear tonofilaments bundles in giant cells confirming their epithelial nature. Furthermore, giant cells contained two or more nuclei with heterogeneous size, nucleoplasmic bridges, nuclear buds, and micronuclei. Similar abnormal nucle... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762221 Tue, 01 Jan 2008 05:00:00 +0100 1762221 http://www.medworm.com/index.php?rid=1762220&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18300034%26dopt%3DAbstract This study shows heterogeneous LM lesions in pure LCDD cases. LM alone may be suspicious but not diagnostic of LCDD. Immunofluorescence is more sensitive than EM for detection of LC for the definitive diagnosis of LCDD. This study supports the importance of utilizing kappa and lambda stains in the routine IF panel for diagnosis of LCDD. PMID: 18300034 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762220 Tue, 01 Jan 2008 05:00:00 +0100 1762220 http://www.medworm.com/index.php?rid=1762219&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18300035%26dopt%3DAbstract Authors: Tsiatis AC, Atkinson JB, Wright JK, Cates JM Sarcomas of the adult liver are unusual neoplasms, and can sometimes pose a difficult differential diagnosis. The authors report a myxoid spindle cell tumor arising in the liver of a 26-year-old woman. Histopathologic, immunohistochemical, and ultrastructural analysis demonstrated features of smooth muscle differentiation. Neoplastic nuclei were positive for estrogen receptor-beta and androgen receptor, but not estrogen receptor-alpha or progesterone receptor. Based on the large size of the tumor and the presence of conspicuous mitotic activity, the diagnosis of myxoid leiomyosarcoma was made. This case represents the third documented example of this tumor in the liver. The differential diagnosis in relation to this particular site ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762219 Tue, 01 Jan 2008 05:00:00 +0100 1762219 http://www.medworm.com/index.php?rid=1762229&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098053%26dopt%3DAbstract This study demonstrates that B. henselae adheres to and invades mature human erythrocytes. The results favor the possibility that erythrocytes can serve as a primary target in Bartonella spp. infections. From this observation, further studies are warranted to prevent Bartonella spp. transfusional transmission. PMID: 18098053 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762229 Thu, 01 Nov 2007 04:00:00 +0100 1762229 http://www.medworm.com/index.php?rid=1762228&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098054%26dopt%3DAbstract Authors: Velho PE, Pimentel V, Del Negro GM, Okay TS, Diniz PP, Breitschwerdt EB Bartonella spp. constitute emerging pathogens of worldwide distribution. Bacillary angiomatosis is the most frequent skin manifestation of bartonelloses; nevertheless, B. henselae infection should always be considered systemic, especially in immunodeficient individuals. The authors report the case of an AIDS patient with bacillary angiomatosis, who had concurrent severe anemia, hepatitis, peritonitis, pleuritis, and pericarditis. Clinical manifestation, electronic microscopic examination of erythrocytes, and histopathology of a papule biopsy suggested a Bartonella sp. infection. Multiple genes were target by PCR and B. henselae DNA was amplified and sequenced (GenBank accession number EF196804) from the an... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762228 Thu, 01 Nov 2007 04:00:00 +0100 1762228 http://www.medworm.com/index.php?rid=1762227&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098055%26dopt%3DAbstract Authors: Jonas L, Bloch C, Zimmermann R, Stadie V, Gross GE, Schäd SG Five patients with generalized slate-gray discoloration of the skin have been diagnosed histologically as argyria in the last 35 years in the Department of Dermatology and Venereology of Rostock and Halle. Light microscopically, there was visible black pigmentation in histiocytes, fibroblasts, and multinucleated giant cells of the dermis. In the transmission electron microscope (TEM), the authors observed electron-dense deposits inside lysosomes and residual bodies of phagocytes as well as outside the cells in the connective matrix. These deposits were identified by elemental analysis in TEM and electron energy loss spectroscopy (EELS) as well as scanning electron microscope (SEM) and energy dispersive x-ray ana... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762227 Thu, 01 Nov 2007 04:00:00 +0100 1762227 http://www.medworm.com/index.php?rid=1762226&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098056%26dopt%3DAbstract Authors: Ledesma-Montes C, Garcés-Ortíz M, Reyes-Gasga J, Salcido-García JF, Hernández-Flores F To recognize recently appearing mineralization phenomena, one must study the external surface of the sialoliths, since it is not possible to study them in the central portions of sialoliths. The authors examined the external surface of a sialolith by scanning electron microscopy and analyzed its microstructures. The study revealed the presence of numerous microstructures of different shapes (nodular, laminar, reticular, microgranular, and multinodular) and variable size arranged in a haphazard fashion. The diverse microstructures encountered strongly suggest that different mechanisms of mineralization occur during growth and development of the sialoliths. PMID: 180980... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762226 Thu, 01 Nov 2007 04:00:00 +0100 1762226 http://www.medworm.com/index.php?rid=1762225&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098057%26dopt%3DAbstract Authors: Kummoona R Research on ultrastructural cytopathological changes and apoptosis that occur in jaw lymphoma were done by using electron microscopy and ground sections. The author described this tumor in 1977-1978 as a highly malignant and lethal condition affecting children between 2 and 8 years (mean age 5 years). A duration of illness between 2 and 3 weeks is common and with a general condition of severe toxicity, anemia, and high body temperature. Clinical and pathological features of 24 children with jaw lymphoma seen in the Maxillofacial Unit, Surgical Specialized Hospital, Medical city, Baghdad, are described. Thirteen males and 11 females were included, with a death rate at 91.1%. The morphological characteristics were examined by ground sections. Lymphoblastic lymphoma fe... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762225 Thu, 01 Nov 2007 04:00:00 +0100 1762225 http://www.medworm.com/index.php?rid=1762224&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18098058%26dopt%3DAbstract Authors: Papadopulos F, Spinelli M, Valente S, Foroni L, Orrico C, Alviano F, Pasquinelli G Cooperation between research communities and software-development teams has led to the creation of novel software. The purpose of this paper is to show an alternative work method based on the usage of ImageJ (http://rsb.info.nih.gov/ij/), which can be effectively employed in solving common microscopic and ultrastructural image analysis tasks. As an open-source software, ImageJ provides the possibility to work in a free-development/sharing world. Its very "friendly" graphical user interface helps users to manage and edit biomedical images. The on-line material such as handbooks, wikis, and plugins leads users through various functions, giving clues about potential new applications. ImageJ is not ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762224 Thu, 01 Nov 2007 04:00:00 +0100 1762224 http://www.medworm.com/index.php?rid=1762235&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963181%26dopt%3DAbstract Authors: Ru YX, Zhao SX, Liu EB, Yang QY, Liu JH, Pang TX, Chen HS There are few transmission electron microscopic studies on bone marrow biopsies of patients with hematological disease owing to the difficulty of overcoming the artifacts of decalcification. Following the fixation of bone marrow biopsies thoroughly before a mild decalcification procedure, ultrastructural studies were performed on 13 patients with varied hematological diseases. Notable features included blood cell disorganization, fibroblast activation, myofibroblast transformation, as well as accumulation of collagen and extracellular amorphous matrix. In addition, excessive blood cell death in leukemia, apoptosis, and macrophage phagocytosis in myelodysplastic syndrome and polycythemia vera, as well as degranulation of... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762235 Sat, 01 Sep 2007 04:00:00 +0100 1762235 http://www.medworm.com/index.php?rid=1762234&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963182%26dopt%3DAbstract This study demonstrates that electron microscopy of skin biopsy is a useful diagnostic method to identify patients with clinical features of osteopetrosis with neuronal storage disease. PMID: 17963182 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762234 Sat, 01 Sep 2007 04:00:00 +0100 1762234 http://www.medworm.com/index.php?rid=1762233&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963183%26dopt%3DAbstract Authors: Rankin HV, Moody AJ, Moate RM, Macnaughton PD, Rahamim J, Smith ME, Sneyd JR The effect of hyperoxia on ciliary abundance in cultured explants of adult human bronchus was investigated. Bronchus samples were removed during surgery from patients receiving pneumonectomy or lobectomy for malignancy. Part or all of each of these samples was used for measurement of cilial abundance by scanning electron microscopy (SEM); in many cases the remainder was subdivided and cultured at 37 degrees C in DMEM medium, maintaining an air interface at the ciliated surface of each segment. Cultured segments were exposed to normoxia or hyperoxia (95% O(2)), and a segment was removed every other day for quantification of cilial abundance by SEM. There was a significant inverse relationship between s... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762233 Sat, 01 Sep 2007 04:00:00 +0100 1762233 http://www.medworm.com/index.php?rid=1762232&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963184%26dopt%3DAbstract Authors: Houreih MA, Eyden B, Deolekar M, Banerjee S The authors report a case of low-grade retroperitoneal malignant peripheral nerve sheath tumor (MPNST) showing Schwannian and fibroblastic differentiation in individual tumor cells. The tumor was detected in a 29-year-old male and posed diagnostic difficulty because of the unusual morphologic and immunophenotypic features. Morphologic examination of the H&E sections revealed a rather circumscribed, highly vascular, moderately cellular spindle cell tumor. The neoplastic cells were arranged in vague, short fascicles, distributed haphazardly amid hemangiopericytoma-like vascular channels, and showed occasional whorls. Myxoid stroma and keloid-like collagen bundles were frequently seen. There were satellite nodules outside the main t... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762232 Sat, 01 Sep 2007 04:00:00 +0100 1762232 http://www.medworm.com/index.php?rid=1762231&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963185%26dopt%3DAbstract Authors: Lach B, Benoit BG The authors describe a mixed malignant dural tumor composed of meningioma and myofibroblastic sarcoma (MFS). The meningioma component displayed epithelial membrane immunoreactivity and interdigitating cellular processes with desmosomal junctions on electron microscopy. MFS cells were immunoreactive for smooth muscle actin and vimentin, and focally for factor XIIIa, CD31, CD34, and Ulex europeus lectin receptors. Electron microscopy showed collections of intermediate filaments, stress fibers, subsarcolemmal densities of microfilaments, occasional fibronexus fibrils, few pinocytic vesicles, and discontinuous external lamina. After gross total removal, the tumor recurred 1 year later as aggressive MFS only. Development of MFS in continuity with meningioma sugges... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762231 Sat, 01 Sep 2007 04:00:00 +0100 1762231 http://www.medworm.com/index.php?rid=1762230&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17963186%26dopt%3DAbstract Authors: Stirling JW, Curry A PMID: 17963186 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762230 Sat, 01 Sep 2007 04:00:00 +0100 1762230 http://www.medworm.com/index.php?rid=1762244&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786825%26dopt%3DAbstract Authors: Mierau GW, Goin L To assess the diagnostic potential of perivascular elastic fiber detection as an indicator of ependymoma, the authors performed ultrastructural studies on a large series of pediatric brain tumors. Elastic fibers were demonstrated by electron microscopy in 38 of 50 (76%) ependymomas, 2 of 25 (8%) choroid plexus tumors, 0 of 100 (0%) medulloblastomas, and 0 of 100 (0%) astrocytomas. In some poorly differentiated examples, elastic fibers were initially the sole indicator of ependymal differentiation. The authors conclude that the sensitivity and specificity of this feature is sufficient to render demonstration of perivascular elastic fibers a useful diagnostic indicator of ependymoma. PMID: 17786825 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pat... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762244 Sun, 01 Jul 2007 04:00:00 +0100 1762244 http://www.medworm.com/index.php?rid=1762243&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786826%26dopt%3DAbstract The objective of this study was to report morphological and functional evidence of a well-preserved preantral follicle recovered from human frozen-thawed ovarian tissue in a long-term culture. The tissue was originally obtained from a 26-year-old woman with breast cancer. The ovarian cortex was collected by laparoscopy and frozen/thawed and cultured for 32 weeks in minimum essential medium alpha-MEM, supplemented with insulin transferrine selenite (ITS), human serum (HS), antibiotics, follicle-stimulating hormone (FSH). and N-acetyl cysteine (NAC). Thawed tissue samples were examined by light microscopy (LM), transmission electron microscopy (TEM), and real-time RT-PCR. LM examination of cortical pieces after 32 weeks of culture showed a healthy early preantral follicle; TEM and real-time ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762243 Sun, 01 Jul 2007 04:00:00 +0100 1762243 http://www.medworm.com/index.php?rid=1762242&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786827%26dopt%3DAbstract Authors: Voloudakis GE, Baltatzis GE, Agnantis NJ, Arnogianaki N, Misitzis J, Voloudakis-Baltatzis I Mucinous carcinoma of the breast (MCB) is histologically classified into 2 groups: (1) pure MCB and (2) mixed MCB. Pure MCB carries a better diagnosis than mixed MCB. This research relates to the cell surface topography and ultrastructure of the cells in the above cases and aims to find the differences between them, by means of two methods: scanning electron microscopy (SEM) and transmission electron microscopy (TEM). For the SEM examination, it was necessary to initially culture the MCB tissues and then proceed with the usual SEM method. In contrast, for the TEM technique, MCB tissues were initially fixed followed by the classic TEM method. The authors found the topography of pure MCB ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762242 Sun, 01 Jul 2007 04:00:00 +0100 1762242 http://www.medworm.com/index.php?rid=1762241&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786828%26dopt%3DAbstract Authors: Worawongvasu R By light microscopy, the dysplastic oral epithelia due to the neoplastic processes are similar to epithelial changes due to the inflammatory processes. Scanning electron microscopy may elucidate the different surface changes between the two. The aim of this study was to examine the surface appearances of the dysplastic oral epithelia adjacent to oral squamous cell carcinoma to see if there are any surface changes. A total of 2 specimens, one specimen from each patient with oral squamous cell carcinoma, were used for this study. Each specimen was divided in two. One half was prepared for light microscopy and the other half was prepared for scanning electron microscopy. Light microscopically, the epithelia showed mild dysplasia. By scanning electron microscopy, th... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762241 Sun, 01 Jul 2007 04:00:00 +0100 1762241 http://www.medworm.com/index.php?rid=1762240&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786829%26dopt%3DAbstract Authors: Worawongvasu R The oral epithelia may show epithelial changes induced by the inflammation of the underlying lamina propria. Light microscopically, the epithelial changes are similar to epithelial dysplasia seen in a premalignant lesion. A scanning electron microscope permits a resolution higher than that of a light microscope. Therefore, it may elucidate the changes observed light microscopically. The purpose of this study was to examine the surface changes of the epithelia of parulides (gum boils) compared with those of normal oral epithelia to see if there were any surface changes due to the underlying inflammatory processes. A total of 3 specimens (1 buccal mucosa, 1 gingiva, and 1 hard palate) taken from 3 patients, one specimen from each patient, were used as controls. A ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762240 Sun, 01 Jul 2007 04:00:00 +0100 1762240 http://www.medworm.com/index.php?rid=1762239&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786830%26dopt%3DAbstract Authors: Hemmi A, Osaka S, Sumie O, Nemoto N, Ohno N, Terada N, Fujii Y, Ohno S A middle-aged Japanese woman visited the Orthopedics Department of Nihon University Nerima Hikarigaoka Hospital complaining of pain in the left hip joint that had started approximately 8 months earlier. Following several examinations, including imaging diagnoses, an incisional biopsy demonstrated a malignant acetabular bone tumor, which was removed and examined by a quick-freezing and deep-etching (QF-DE) method, conventional electron microscopy, and light microscopy. Histologically, the tumor was a chondrosarcoma with marked myxoid changes. An interesting extracellular matrix was observed by the QF-DE method. The myxoid area consisted of a fine meshwork of proteoglycans (PG) without obvious aggrecans, whic... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762239 Sun, 01 Jul 2007 04:00:00 +0100 1762239 http://www.medworm.com/index.php?rid=1762238&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786831%26dopt%3DAbstract Authors: Seretis EC, Gavriil AN, Golematis VC, Voloudakis-Baltatzis IE The aim of this study was to investigate the ultrastructural appearance of pancreatic adenocarcinoma combined with glucagon and gastrin/cholecystokinin (CCK) expression. The authors investigated the ultrastructure and the immunocytochemistry of 12 human pancreatic cancer specimens and used 3 chronic pancreatitis samples and 6 adjacent histological normal pancreatic tissues (away from the tumor) as controls. The ultrastructural study revealed that chronic pancreatitis tissues were characterized by alterations of the secretory cells. The enzymic and secretory changes were confirmed by electron immunogold results. Glucagon appeared to be located not only in islet alpha cells but also in intermediate alpha acinar cells.... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762238 Sun, 01 Jul 2007 04:00:00 +0100 1762238 http://www.medworm.com/index.php?rid=1762237&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786832%26dopt%3DAbstract This article is one of the few detailed clinicopathological documentations of a small cell malignant melanoma of the rectum (anorectum) and the first to have the diagnosis confirmed ultrastructurally by the identification of melanosomes. The present case adds to the 3 mainly or entirely small cell anorectal malignant melanomas described in the literature. There are also at least 12 other cases with less well-defined numbers of small tumor cells or with small cells admixed with other cell morphologies. Documentation of these unusual morphological variants is important for identifying any distinctive outcome they might exhibit compared with conventional malignant melanoma. PMID: 17786832 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762237 Sun, 01 Jul 2007 04:00:00 +0100 1762237 http://www.medworm.com/index.php?rid=1762236&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17786833%26dopt%3DAbstract The objective of this research was to evaluate, by scanning electron microscopy, the apical structure of extracted human permanent teeth with different degrees of pulp and periapical pathology. A total of 25 teeth were extracted: 5 teeth with vital pulp (group I); 10 teeth with pulp necrosis without radiographically visible periapical lesion (group II); 10 teeth with pulp necrosis with radiographically visible periapical lesion (group III). The root apex was sectioned and processed for scanning electron microscopy. In groups I and II, fibers covered the root cementum and there was no cementum resorption or microorganisms. There were areas of cementum resorption in group III with microorganisms on the root apex surface (biofilm) and no fibers. The authors conclude that the presence of chron... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762236 Sun, 01 Jul 2007 04:00:00 +0100 1762236 http://www.medworm.com/index.php?rid=1762251&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613997%26dopt%3DAbstract Authors: Iancu TC, Mahajnah M, Manov I, Shaoul R Microvillous inclusion disease (MVID) is a congenital, usually neonatal, autosomal recessive condition manifested by severe, prolonged secretory diarrhea. Intestinal biopsies reveal extensive microvilli abnormalities, typical inclusions and vesicles mainly of the apical-luminal enterocytes and colonocytes. Although diagnosis can be suspected by special stains of the mucosa (PAS, CD10), the definitive diagnosis, recommended in view of potential intestinal transplantation, requires electron microscopy. In view of the marked variability of ultrastructural changes, extensive illustration is considered valuable for diagnosis. While the pathogenesis is still unknown, a number of images illustrate the suspected "arrested-trafficking" hypothesis... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762251 Tue, 01 May 2007 04:00:00 +0100 1762251 http://www.medworm.com/index.php?rid=1762250&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613998%26dopt%3DAbstract Authors: Iancu TC, Mahajnah M, Manov I, Cherurg S, Knopf C, Mandel H A new group of genetic diseases characterized by defective glycoprotein biosynthesis was recently described. Transferrin isoelectric focusing enabled identification of several types of patients with congenital disorders of glycosylation (CDG). The authors report on the liver involvement in two siblings with CDG type Ix presenting with failure to thrive and hypertransaminasemia who developed cardiomyopathy. In the initially affected infant, liver biopsy at 13 months of age showed increased periportal cellularity, steatosis, and mild fibrosis. Ultrastructurally, the hepatocytes displayed numerous myelinosomes, mostly with a pericanalicular polarization. No myelinosomes were seen in the bile canaliculi, Kupffer cells, an... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762250 Tue, 01 May 2007 04:00:00 +0100 1762250 http://www.medworm.com/index.php?rid=1762249&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613999%26dopt%3DAbstract Authors: Latif S, Zaman F, Veeramachaneni R, Jones L, Uribe-Uribe N, Turbat-Herrera EA, Herrera GA Reactivation of BK polyomavirus (BKV) is increasingly recognized as a cause of failure of renal allografts. Since no specific treatment is available for this infection, early diagnosis is important, as it allows for early intervention and possible recovery of renal function. Forty-four consecutive renal transplant biopsies performed over a 2-year period were included in the study. In addition to evaluation of renal biopsy tissue sections using routine histochemical stains, CD3, CD20, BK virus immunostains using the specific BK virus and the SV40 antibodies and electron microscopy studies were performed. None of the transplant cases but one exhibited classical histologic viral changes. Vir... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762249 Tue, 01 May 2007 04:00:00 +0100 1762249 http://www.medworm.com/index.php?rid=1762248&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17614000%26dopt%3DAbstract Authors: Yamazaki K Elastofibromas have been described as ill-defined tumors, composed of fibroblastic stromal cells and a dense collagenous stroma. A total of 5 elastofibromas from 4 Japanese patients were examined by ultrastructural and immunohistochemical methods. The proliferating fibroblastic stromal cells in the lesion showed Cx43-type gap junctions, isolated cilia, prominent nuclear fibrous laminae, and primitive cellular junctions with incomplete laminae. The active proliferating fibroblastic cells showed positive staining for vimentin, CD34, factor XIIIa, prominin 2 (CD133), and MEF 2. Conspicuous cell-to-matrix interactions were observed with abnormally unique elastins, collagens (type I, III, and IV), laminin, fibronectin, and amorphous extracellular matrix (GAGs; glycosamin... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762248 Tue, 01 May 2007 04:00:00 +0100 1762248 http://www.medworm.com/index.php?rid=1762247&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17614001%26dopt%3DAbstract Authors: Uhlík J, Vajner L, Adásková J, Konrádová V Inhaled corticosteroids are being recommended for the treatment of bronchial asthma for their anti-inflammatory properties and reduction of airway hyperreactivity. The first tissue coming to the contact with all inhaled substances is the airway epithelium. In this experiment, the immediate effect of a single MDI dose of beclomethasone on the ultrastructure of the tracheal and bronchiolar epithelium was studied. Due to the beclomethasone administration, the secretory elements were highly affected. The tracheal goblet cells were damaged, mucus release was significantly accelerated, and the mechanism of secretion was influenced. The bronchiolar Clara cells revealed signs of the pathological alteration. Their secr... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762247 Tue, 01 May 2007 04:00:00 +0100 1762247 http://www.medworm.com/index.php?rid=1762246&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17614002%26dopt%3DAbstract Authors: Hirose T, Shimada S, Tani T, Hasegawa T Ossifying fibromyxoid tumor (OFMT) is a rare enigmatic soft tissue tumor, the origin of which is still uncertain. The authors report on 3 cases of OFMT arising in the trunk and head and neck regions of adults. Two recurred and one was suspected to have metastasis. All tumors consisted of multiple nodules, in which round or polygonal tumor cells were arranged in sheets or cords within a fibromyxoid background. Characteristic shell-like bone tissues were recognized in all tumors. Based on the grading system proposed by Folpe et al., 2 cases were designated as malignant OFMT and 1 as typical. In addition to S-100 protein, cytokeratin and neuronal markers (neurofilament, CD56 or CD57) were detected in 1 and 2 tumors, respectively. The salien... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762246 Tue, 01 May 2007 04:00:00 +0100 1762246 http://www.medworm.com/index.php?rid=1762245&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17614003%26dopt%3DAbstract Authors: Kim JH, Cho BK, Kim IO, Park SH A case of clear-cell ependymoma occurring in the cerebellum of a 3-year-old girl is reported. Light-microscopically, the tumor consisted mainly of clear cells with a perinuclear halo and showed some vague perivascular pseudorosettes, not true rosettes. In addition, the histological features of anaplasia, characterized by increased mitosis and focal pseudopalisading necrosis, were also observed. Immunohistochemically, the tumor cells were focally positive for glial fibrillary acidic protein and weakly positive for epithelial membrane antigen. Ultrastructurally, the intermediate junctions and rudimentary cilia confirmed the ependymal differentiation. Fifteen cases of infratentorial clear-cell ependymoma have been reported to date, and this case is... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762245 Tue, 01 May 2007 04:00:00 +0100 1762245 http://www.medworm.com/index.php?rid=1762258&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613990%26dopt%3DAbstract Authors: Pretorius E, Humphries P The coagulation process, including thrombin, fibrin, as well as platelets, plays an important role in hemostasis, contributing to the general well-being of humans. Fibrin formation and platelet activation are delicate processes that are under the control of many small physiological events. Any one of these many processes may be influenced or changed by external factors, including pharmaceutical or nutritional products, e.g., the sweetener aspartame (L-aspartyl-L-phenylalanine methyl ester). It is known that phenylalanine is present at position P(9) and aspartate at position P(10) of the alpha-chain of human fibrinogen, and plays an important role in the conversion of fibrinogen to fibrin by the catalyst alpha-thrombin. The authors investigate the effec... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762258 Thu, 01 Mar 2007 05:00:00 +0100 1762258 http://www.medworm.com/index.php?rid=1762257&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613991%26dopt%3DAbstract Authors: González MA, Lois CG The current WHO classification of soft tissue tumors is based on the lineage of differentiation of the proliferating cells. Since mature mesenchymal cells have a broad phenotypic plasticity it has been considered unnecessary to recur to a hypothetical stem cell to explain the origin of these neoplasms. In spite of this assumption, the target cell of the oncogenic mutations in mesenchymal tumors is still a controversial item. Myxoid mesenchymal tumors constitute a heterogeneous group of neoplasms sharing in an ample mucinous matrix that separates neoplastic cells and facilitates their single submicroscopic study under electron microscopy examination. The authors have studied, by electron microscopy, 74 myxoid mesenchymal tumors, including a large varie... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762257 Thu, 01 Mar 2007 05:00:00 +0100 1762257 http://www.medworm.com/index.php?rid=1762256&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613992%26dopt%3DAbstract Authors: Dodson RF, Shepherd S, Levin J, Hammar SP Inhaled dust particulates are able to relocate to the extrapulmonary compartments, particularly the lymph nodes that drain the lung. There is little information about the concentration and type of asbestos in the lymphatics and lymph nodes. Quantitative analysis of asbestos lymph node burden conducted by light and analytical transmission electron microscopy has shown ferruginous bodies in lymph nodes that drain the lung and appreciable numbers of short asbestos fibers accumulate in lymph nodes in occupationally exposed individuals. The location of lymph nodes in the thoracic cavity was categorized according to the Naruke anatomical map. Tissue from eleven individuals with a history of asbestos exposure were selected for a comparative s... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762256 Thu, 01 Mar 2007 05:00:00 +0100 1762256 http://www.medworm.com/index.php?rid=1762255&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613993%26dopt%3DAbstract Authors: Ru YX, Mi YC, Liu JH, Cui W, Wang HJ, Zhao SX, Jian-Xiang W The ribosome-lamella complex (RLC) is a cylindrical structure composed of annular lamella associated particles, regarded as ribosomes, around a central core, which is best known in hairy cell leukemia. RLC has been presumed to originate from aggregating rER and ribosomes. Incomplete and maturing RLC structures have been called RLC precursors (pre-RLC). The present paper investigates the various architectural aspects of pre-RLC and the ultrastructural characteristics of the blasts in 6 cases of acute monocytic leukemia (M5) in which these structures occur. Blasts bearing pre-RLC contained irregular nuclei with less heterochromatin and a prominent nucleolus, and many cytoplasmic organelles in an abundant cytoplasm. The ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762255 Thu, 01 Mar 2007 05:00:00 +0100 1762255 http://www.medworm.com/index.php?rid=1762254&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613994%26dopt%3DAbstract This study shows the lack of characteristic LM findings of LCDD in combined cases of LCDD and LCCN and emphasizes the difficulty for-definitive diagnosis-without IF and EM. PMID: 17613994 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762254 Thu, 01 Mar 2007 05:00:00 +0100 1762254 http://www.medworm.com/index.php?rid=1762253&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613995%26dopt%3DAbstract Authors: Orenstein JM A complex relationship exists between HIV and its cellular targets. The lethal effect of HIV on circulating CD4(+) helper T lymphocytes parallels the degree of the infected individual's immunodeficiency and ultimately the transition to AIDS and death. However, as with other members of the Lentivirus family of retroviruses, the ubiquitous, mobile macrophage is also a prime target for HIV infection, and apparently, in most instances, is the initial infected cell, since most people are infected with a CCR5 chemokine-tropic virus. Unlike the lymphocyte, the macrophage is apparently a more stable viral host, capable of a long infected life as an HIV reservoir and a chronic source of infectious virus. Published in vitro studies have indicated that whereas lymphocytes re... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762253 Thu, 01 Mar 2007 05:00:00 +0100 1762253 http://www.medworm.com/index.php?rid=1762252&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17613996%26dopt%3DAbstract Authors: Finzi G, Franzi F, Sessa F, Mastaglio C, Capella C The presence of Tropheryma whippelii was demonstrated in the PAS-negative mesenteric granulomatous lymph nodes of a patient affected by Whipple disease. Ultrastructurally a few bacteria, enclosed by a membrane characteristic of Tropheryma whippelii, were found in the extracellular spaces and remnants of bacteria were found in the phagocytic vacuoles of macrophages. The scarce number of bacilli, probably due to the fact that the disease was at an initial phase, could explain the absence of PAS positivity. This case confirms the role of the electron microscopy in the diagnosis of Whipple disease, especially for extra-intestinal lesions and at the initial phase of the disease, when the characteristic PAS-positive macrophages can ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762252 Thu, 01 Mar 2007 05:00:00 +0100 1762252 http://www.medworm.com/index.php?rid=1762267&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455092%26dopt%3DAbstract Authors: Brealey JK BK virus nephropathy is a known cause of renal transplant dysfunction and failure. The disease is identified by examination of kidney biopsy tissue utilizing histopathological techniques. Ultrastructural examination of two glomeruli revealed pathology within one glomerulus. Glomerular basement membranes contained subepithelial humps of deposit-like material and BK viruses were identified within this material. Viruses were identified within intertubular capillaries. There was evidence of cytoplasmic clearance of viruses from the glomerular basement membrane by podocytes. The findings may be relevant to the investigation of hump formation and antigen clearance in BK virus nephropathy and postinfectious glomerulonephritis. PMID: 17455092 [PubMed - indexed for MEDLI... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762267 Mon, 01 Jan 2007 05:00:00 +0100 1762267 http://www.medworm.com/index.php?rid=1762266&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455093%26dopt%3DAbstract Authors: Sikorska B, Papierz W, Zakrzewki K, Fiks T, Polis L, Liberski PP Gangliogliomas are rare brain tumors, composed of neuronal and glial cells mixed in a different proportion. The basic histopathological pattern of gangliogliomas is well recognized but the variable microscopic appearance still can pose a challenge to the neuropathologist. The authors reanalyzed their series of gangliogliomas in the files of two departments of neuropathology. All analyzed tumors fulfilled the WHO histological criteria of ganglioglioma. Seven tumors were examined by electron microscopy. The following ultrastructural features were graded: presence of dense-cored vesicles, synaptic vesicles, synapses and intermediate filaments, abundant basal membranes, dystrophic neurites, autophagic vacuoles, and m... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762266 Mon, 01 Jan 2007 05:00:00 +0100 1762266 http://www.medworm.com/index.php?rid=1762265&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455094%26dopt%3DAbstract Authors: Fowler DJ, Anderson G, Vellodi A, Malone M, Sebire NJ Some lysosomal storage disorders cause progressive prenatal accumulation of undegradable metabolites that manifest as membrane-bound vacuoles in endothelial cells, fibroblasts, and trophoblast, identifiable by electron microscopic examination of chorionic villus samples (CVS). There were 111 CVS, which had ultrastructural examination for suspected storage disorders at Great Ormond Street Hospital (1988-2005). There were 31 positive diagnoses, including glycogen storage disease type II, gangliosidosis type 1, mucopolysaccharidosis type 1, MPS not specified, Niemann-Pick type A, sialidosis/mucolipidosis type 1, neuronal ceroid lipofuscinoses (including variant forms), Wolman disease, sialic acid storage disease, and storage d... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762265 Mon, 01 Jan 2007 05:00:00 +0100 1762265 http://www.medworm.com/index.php?rid=1762264&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455095%26dopt%3DAbstract In conclusion, this study documents that ultrastructural differences exist among phenotypically similar hMSCs derived from human bone marrow and term placenta leaves; such differences could be revelatory of the hMSCs in vitro differentiation potential and may provide useful clues to attempt their in situ identification. PMID: 17455095 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762264 Mon, 01 Jan 2007 05:00:00 +0100 1762264 http://www.medworm.com/index.php?rid=1762263&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455096%26dopt%3DAbstract Authors: Salaroli R, Russo A, Ceccarelli C, Mina GD, Arcella A, Martinelli GN, Giangaspero F, Capranico G, Cenacchi G Gene mutations impairing the functions of the WNT signaling transduction pathway have been found in approximately 15% of human sporadic medulloblastomas. To understand the functional role of the WNT pathway in medulloblastoma, we have investigated the intracellular distribution of beta-catenin in a series of 17 human medulloblastomas to correlate such expression with neuronal differentiation and in cultured cell models following functional silencing of the APC gene by small-interference RNA (siRNA). Transient siRNA transfection resulted in a 50% reduction of the APC gene product levels in both DAOY and D283MED cell lines. In the former, less-differentiated cell line, be... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762263 Mon, 01 Jan 2007 05:00:00 +0100 1762263 http://www.medworm.com/index.php?rid=1762262&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455097%26dopt%3DAbstract Authors: Kalil RK, Monteiro A, Lima MI, Silveira EB, Foltran FS, Martins CE, Rizzo IM Macrophagic myofasciitis (MMF) is an inflammatory myopathy related to aluminum-containing vaccines. Described in 1998, most cases were reported in adults, with only 22 cases being reported in children. Three children aged between 13 months and 3(1/2) years were investigated in our institution for neuromuscular symptoms. They underwent thorough clinical, familial, and laboratory investigations, electroneuromyography, muscle biopsy with transmission electron microscopy, scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS), and, in one case, brain magnetic resonance imaging. They had received regular immunizations. Two patients were hypotonic and one presented with myotonia. Muscle biops... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762262 Mon, 01 Jan 2007 05:00:00 +0100 1762262 http://www.medworm.com/index.php?rid=1762261&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455098%26dopt%3DAbstract Authors: Yamazaki K, Eyden BP Lipomatous hemangiopericytoma is a rare mesenchymal tumor showing areas of lipid-containing cells admixed with a spindle-cell component. Like other hemangiopericytomas, it shows a similar vascular pattern to solitary fibrous tumor and, partly for this reason, it and other hemangiopericytomas have been subsumed into solitary fibrous tumor. The present study provides a comprehensive documentation of a single case of pulmonary lipomatous hemangiopericytoma of the lung, the first to be described at this site, and compares it with solitary fibrous tumor, in terms of clinical, histological, immunohistochemical, ultrastructural, and cytogenetic findings. Apart from the lipid-laden-cell component, pulmonary lipomatous hemangiopericytoma and solitary fibrous tumor ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762261 Mon, 01 Jan 2007 05:00:00 +0100 1762261 http://www.medworm.com/index.php?rid=1762260&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455099%26dopt%3DAbstract Authors: Kanner WA, Drachenberg CB, Papadimitriou JC, Wang S, Meltzer SJ, Sklar GN Penile malignancies are rare in developed countries. The authors present a case of a penile urethral mesenchymal tumor occurring in a 51-year-old Caucasian male and displaying light microscopic, immunohistochemical, and ultrastructural features suggestive of a pacemaker cell type, combined with a lack of diagnostic features of any other established tumor category. The immunohistochemical profile was intensely positive for vimentin, PKC theta, and NSE and weakly positive to nonreactive for CD34 and smooth muscle actin, and entirely negative for CD117 (c-kit), S-100, and other markers. C-kit and PDGFRA gene analysis showed no mutations. Electron microscopy revealed tumor cells with plentiful cytoplasm and ... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762260 Mon, 01 Jan 2007 05:00:00 +0100 1762260 http://www.medworm.com/index.php?rid=1762259&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17455100%26dopt%3DAbstract Authors: Goedhals J, Beukes CA, Cooper S A 60-year-old female presented with a history of hoarseness, cough, chest pain, and dyspnea and a needle biopsy sample was submitted for histology. Light microscopy showed sheets of tumor cells with eosinophilic cytoplasm containing multiple crystals and eccentrically located nuclei. Electron microscopy showed large intracytoplasmic crystalloid inclusions. No crystalloid structures were found extracellularly. The tumor cells also had long slender microvilli and cell junctions, the features being consistent with a malignant epithelial mesothelioma. In the authors' experience this is a rare finding. The clinical information initially received was poor and electron microscopy was essential in making the correct diagnosis. PMID: 17455100 [PubMed... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762259 Mon, 01 Jan 2007 05:00:00 +0100 1762259 http://www.medworm.com/index.php?rid=1762279&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182431%26dopt%3DAbstract Authors: Agaram NP, Baren A, Antonescu CR Hepatic embryonal (undifferentiated) sarcoma (ES) is a rare pediatric tumor occurring predominantly in the first decade of life, but a few examples of adult ES have also been described. Isolated ultrastructural reports describe contradictory lines of differentiation in these tumors. Four pediatric and 3 adult ES cases were studied ultrastructurally and features were correlated with morphology. Morphologically, tumors were composed of mixture of plump spindle cells and bizarre giant cells, showing abundant cytoplasmic eosinophilic globules. Ultrastructurally, the hallmark features in all cases included dilated RERs and secondary lysosomes with dense precipitates. Dilated mitochondria and mitochondrial-RER complexes were often seen. Other feature... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762279 Wed, 01 Nov 2006 05:00:00 +0100 1762279 http://www.medworm.com/index.php?rid=1762278&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182432%26dopt%3DAbstract This study disclosed for the first time the diagnostic ultrastructures of Wilson disease, which might represent different detoxification processes to the reactive metals of copper and iron. PMID: 17182432 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762278 Wed, 01 Nov 2006 05:00:00 +0100 1762278 http://www.medworm.com/index.php?rid=1762277&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182433%26dopt%3DAbstract Authors: Hammer U, Wegener R, Nizze H, Wöhlke G, Kruse C, Dworniczak B, Kühn-Velten WN, Nöldge-Schomburg G, Hofmockel R, Jonas L A 20-year old African woman underwent anesthesia for interruption of an unwanted pregnancy. As a consequence of the anesthesia, she went into coma because of an as yet unknown and untested homozygotic state of sickle cell anemia. Her vital functions were maintained for more than 1 year by intensive medicine, but she died finally in multiorgan failure and aspiration pneumonia. Because of the complications under anesthesia and the missing preanesthetic test for hemoglobinopathy, autopsy was conducted in the forensic medicine department and not in the department of pathology. The sickle cell disease was diagnosed by electrophoresis of the blood, b... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762277 Wed, 01 Nov 2006 05:00:00 +0100 1762277 http://www.medworm.com/index.php?rid=1762276&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182434%26dopt%3DAbstract Authors: Hoogendijk CF, Marx J, Honey EM, Pretorius E, Christianson AL Zimmermann-Laband syndrome (ZLS) is a very rare autosomal dominant inherited condition characterized by 3 major clinical findings of which gingival hyperplasia are always present. The great heterogenicity of the syndrome is illustrated by the numerous variable clinical findings described in the literature. The purpose of the study was to examine a patient diagnosed with ZLS and to describe possible new characteristics of this rare syndrome, including the ultrastructural morphology using a transmission electron microscope (TEM) of the gingival and dermal fibroblasts. The ultrastrucutral morphology as has not previously been described in the literature. Tissue was collected from the alveolar ridge and skin of the fore... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762276 Wed, 01 Nov 2006 05:00:00 +0100 1762276 http://www.medworm.com/index.php?rid=1762275&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182435%26dopt%3DAbstract This study shows that the ultrastructural features are well developed and can help in distinguishing plasmablastic lymphoma from other light microscopically undifferentiated tumors. PMID: 17182435 [PubMed - indexed for MEDLINE] (Source: Ultrastructural Pathology) Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762275 Wed, 01 Nov 2006 05:00:00 +0100 1762275 http://www.medworm.com/index.php?rid=1762274&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182436%26dopt%3DAbstract Authors: Gallardo-Arrieta F, Mogas T, Magán L, García MA, García F, Abal M, Morote J, Serrano S, Reventós J, Lioreta J Benign prostatic hyperplasia is a prevalent disease that has received relatively little attention in spite of its morbidity and remarkable social impact. There are few animal models of prostatic hyperplasia. The dog is the only species, along with humans, in which prostatic hyperplasia develops spontaneously and almost universally with age. The aim of the present study has been to compare the ultrastructural findings in a model of experimentally induced canine prostatic hyperplasia with those of the spontaneously developed changes in untreated dogs. An experimental group of 5 male beagle dogs were castrated and treated with combined steroids (3 week... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762274 Wed, 01 Nov 2006 05:00:00 +0100 1762274 http://www.medworm.com/index.php?rid=1762273&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182437%26dopt%3DAbstract The objective of this study was to investigate the relationship between ultrastructural features and genotype in a large series of 125 histologically confirmed and CD117 positive GISTs. PCR analysis for the presence of KIT exon 9, 11, 13, and 17 and PDGFRA exon 12 and 18 mutations was performed. There were 62 (50%) tumors located in the stomach and 45 (36%) in the small bowel. Overall, KIT mutations were detected in 93 (75%) patients: 86 (69%) in exon 11, and 7 (6%) in exon 9. A PDGFRA mutation was detected in 7 (6%) cases and 25 (19%) cases had no mutation. Ultrastructurally, skeinoid fibers were seen in 55 (44%) cases and were more common in small bowel than stomach GISTs, and occurred in only in 1 of 16 patients with an ITD (KIT) exon 11 or PDGFRA mutation. Focal actin microfilaments we... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762273 Wed, 01 Nov 2006 05:00:00 +0100 1762273 http://www.medworm.com/index.php?rid=1762272&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182438%26dopt%3DAbstract Authors: Cuccurullo L, Accardo M, Agozzino L, Blasi F, Esposito S, Vosa C Protecting the myocardium from the risk of acute ischemia during heart surgery is still an unsolved problem; the problem is even more open and more pressing in pediatric heart surgery. To meet this greater risk it is advisable to use a cardioplegic solution with a composition that is better suited to the particular morphofunctional conditions of the myocardium in the child, i.e., a solution offering greater protection. To this purpose the authors experimented with Celsior cardioplegic solution during heart surgery in children to evaluate the efficacy compared to the standard St. Thomas solution. In this comparative study 15 children were treated with Celsior cardioplegic solution and 15 others with St. Thomas car... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762272 Wed, 01 Nov 2006 05:00:00 +0100 1762272 http://www.medworm.com/index.php?rid=1762271&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182439%26dopt%3DAbstract Authors: Politi PK, Havaki S, Manta P, Lyritis G The regeneration of soleus muscle injury induced by the bupivacaine model was studied ultrastructurally and immunohistochemically. Twenty-one young (age range 3-3.5 months) male Wistar rats were subjected to a single intramuscular injection of 1 mL of 0.5% Marcaine. The muscles were examined on biopsy days 1, 2, 3, 5, 7, 14, and 21. By day 1, mononuclear inflammatory cells had invaded the necrotic sarcoplasm. Degenerative morphological findings counted mainly for the hypercontracted fibers, dilation of sarcoplasmic reticulum, plasma membrane defects, mitochondrial alterations, and myofibril discontinuities. By day 2 proliferating myoblasts were seen with variety in shape, which fused on the day 3. Myotubes with multiple central nuclei an... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762271 Wed, 01 Nov 2006 05:00:00 +0100 1762271 http://www.medworm.com/index.php?rid=1762270&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182440%26dopt%3DAbstract Authors: Herrera GA In the last two decades, the ability of mesangial cells to respond to various stimuli or injurious agents by altering their phenotype and function has become recognized. The plasticity of these mesangial cells has been linked to the morphological and functional alterations responsible for the pathologic findings. Many of the glomerular disorders target the mesangium as the primary and/or initial site of injury. Understanding how mesangial cells are altered in the various conditions provides a platform for conceptualizing pathologic mechanisms and defining key steps amenable to therapeutic intervention. The present paper reviews the normal and altered mesangium with an emphasis on mechanisms involved in alterations of mesangial homeostasis. Mesangial cells and matrix... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762270 Wed, 01 Nov 2006 05:00:00 +0100 1762270 http://www.medworm.com/index.php?rid=1762269&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17182441%26dopt%3DAbstract In this report, the authors summarize their 19-year experience with over 200 biochemically proven cases of lysosomal storage diseases using electron microscopic screening of more than 950 skin biopsies. They found that electron microscopy (EM) is a highly sensitive, efficient, cost-effective, and rapid diagnostic screening tool for evaluation of lysosomal storage diseases in skin biopsies. Although EM is more expensive than a single enzyme assay, it can exclude more than 90% of cases in which lysosomal storage disease is being considered. EM is critical for diagnosis of neuronal ceroid lipofuscinosis and mucolipidosis IV and is the most cost-effective screening tool in patients with previously unrecognized storage diseases. PMID: 17182441 [PubMed - indexed for MEDLINE] (Source: Ultrast... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762269 Wed, 01 Nov 2006 05:00:00 +0100 1762269 http://www.medworm.com/index.php?rid=1762268&cid=s_28425_32_f&fid=28425&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17183762%26dopt%3DAbstract Authors: Bhattacharjee M, Venugopal B, Wong KT, Goto YI, Bhattacharjee MB The authors describe the case of a 50-year-old man with chronic progressive external ophthalmoplegia (CPEO), diabetes mellitus (DM), and coronary artery disease. The patient had no cardiac conduction abnormalities. During coronary artery bypass surgery, his heart and two skeletal muscles were biopsied. All three muscles showed ragged red fibers. The heart muscle showed significant glycogen accumulation. Analysis of mitochondrial DNA (mtDNA) showed a 5019-base-pair deletion, with no duplications. There were morphologically abnormal mitochondria in all 3 muscles, with clinically apparent difference in preservation of function. The combination of diabetes mellitus and mtDNA deletion is fortuitous, as they can be cau... Ultrastructural Pathology journals http://www.medworm.com/rss/comments.php?id=1762268 Wed, 01 Nov 2006 05:00:00 +0100 1762268