MedWorm: Biology

A monograph of the entomopathogenic genera hypocrella, moelleriella, and samuelsia gen. nov. (ascomycota, hypocreales, clavicipitaceae), and their aschersonia-like anamorphs in the neotropics.

Studies in Mycology — Thu, 03 Jul 2008 14:21:14 +0100

A monograph of the entomopathogenic genera Hypocrella, Moelleriella, and Samuelsia gen. nov. (Ascomycota, Hypocreales, Clavicipitaceae), and their aschersonia-like anamorphs in the Neotropics.

Stud Mycol. 2008;60:1-66

Authors: Chaverri P, Liu M, Hodge KT

The present taxonomic revision deals with Neotropical species of three entomopathogenic genera that were once included in Hypocrella s. l.: Hypocrella s. str. (anamorph Aschersonia), Moelleriella (anamorph aschersonia-like), and Samuelsia gen. nov (anamorph aschersonia-like). Species of Hypocrella, Moelleriella, and Samuelsia are pathogens of scale insects (Coccidae and Lecaniidae, Homoptera) and whiteflies (Aleyrodidae, Homoptera) and are common in tropical regions. Phylogenetic analyses of DNA sequences from nuclear ribosomal large subunit (28S), translation elongation factor 1-alpha (TEF 1-alpha), and RNA polymerase II subunit 1 (RPB1) and analyses of multiple morphological characters demonstrate that the three segregated genera can be distinguished by the disarticulation of the ascospores and shape and size of conidia. Moelleriella has filiform multi-septate ascospores that disarticulate at the septa within the ascus and aschersonia-like anamorphs with fusoid conidia. Hypocrella s. str. has filiform to long-fusiform ascospores that do not disarticulate and Aschersonia s. str. anamorphs with fusoid conidia. The new genus proposed here, Samuelsia, has filiform to long-fusiform ascospores that do not disarticulate and aschersonia-like anamorphs with small allantoid conidia. In addition, the present study presents and discusses the evolution of species, morphology, and ecology in Hypocrella, Moelleriella, and Samuelsia based on multigene phylogenetic analyses.

PMID: 18490956 [PubMed - in process]

(Source: Studies in Mycology)

MedWorm Sponsored Message: Find out how you can get your message across here by sponsoring this MedWorm news feed.

The ppar-γ agonist 15-deoxy-Δ12,14-prostaglandin j2 attenuates microglial production of il-12 family cytokines: potential relevance to alzheimer's disease

PPAR Research — Thu, 03 Jul 2008 11:56:39 +0100

Accumulation of amyloid-β peptide (Aβ) appears to contribute to the pathogenesis of Alzheimer's disease (AD). Therapeutic hope for the prevention or removal of Aβ deposits has been placed in strategies involving immunization against the Aβ peptide. Initial Aβ immunization studies in animal models of AD showed great promise. However, when this strategy was attempted in human subjects with AD, an unacceptable degree of meningoencephalitis occurred. It is generally believed that this adverse outcome resulted from a T-cell response to Aβ. Specifically, CD4+ Th1 and Th17 cells may contribute to severe CNS inflammation and limit the utility of Aβ immunization in the treatment of AD. Interleukin (IL)-12 and IL-23 play critical roles in the development of Th1 and Th17 cells, respectively. In the present study, Aβ1−42 synergistically elevated the expression of IL-12 and IL-23 triggered by inflammatory activation of microglia, and the peroxisome proliferator-activated receptor (PPAR)-γ agonist 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2) effectively blocked the elevation of these proinflammatory cytokines. Furthermore, 15d-PGJ2 suppressed the Aβ-related synergistic induction of CD14, MyD88, and Toll-like receptor 2, molecules that play critical roles in neuroinflammatory conditions. Collectively, these studies suggest that PPAR-γ agonists may be effective in modulating the development of AD. (Source: PPAR Research)

The role of ppar-γ and its interaction with cox-2 in pancreatic cancer

PPAR Research — Thu, 03 Jul 2008 11:56:39 +0100

In recent years, the study of the peroxisome proliferators activated receptor gamma (PPAR-γ) as a potential target for cancer prevention and therapy has gained a strong interest. However, the overall biological significance of PPAR-γ in cancer development and progression is still controversial. While many reports documented antiproliferative effects in human cancer cell and animal models, several studies demonstrating potential tumor promoting actions of PPAR-γ ligands raised considerable concerns about the role of PPAR-γ in human cancers. Controversy also exists about the role of PPAR-γ in human pancreatic cancers. The current review summarizes the data about PPAR-γ in pancreatic cancer and highlights the biologically relevant interactions between the cyclooxygenase and PPAR system. (Source: PPAR Research)

Pparγ and apoptosis in cancer

PPAR Research — Thu, 03 Jul 2008 11:56:39 +0100

Peroxisome proliferator-activated receptors (PPARs) are ligand binding transcription factors which function in many physiological roles including lipid metabolism, cell growth, differentiation, and apoptosis. PPARs and their ligands have been shown to play a role in cancer. In particular, PPARγ ligands including endogenous prostaglandins and the synthetic thiazolidinediones (TZDs) can induce apoptosis of cancer cells with antitumor activity. Thus, PPARγ ligands have a potential in both chemoprevention and therapy of several types of cancer either as single agents or in combination with other antitumor agents. Accordingly, the involvement of PPARγ and its ligands in regulation of apoptosis of cancer cells have been extensively studied. Depending on cell types or ligands, induction of apoptosis in cancer cells by PPARγ ligands can be either PPARγ-dependent or -independent. Through increasing our understanding of the mechanisms of PPARγ ligand-induced apoptosis, we can develop better strategies which may include combining other antitumor agents for PPARγ-targeted cancer chemoprevention and therapy. This review will highlight recent research advances on PPARγ and apoptosis in cancer. (Source: PPAR Research)

A novel mechanism of pparγ regulation of tgfβ1: implication in cancer biology

PPAR Research — Thu, 03 Jul 2008 11:56:39 +0100

Peroxisome proliferator-activated receptor-γ (PPARγ) and retinoic acid X-receptor (RXR) heterodimer, which regulates cell growth and differentiation, represses the TGFβ1 gene that encodes for the protein involved in cancer biology. This review will introduce the novel mechanism associated with the inhibition of the TGFβ1 gene by PPARγ activation, which regulates the dephosphorylation of Zf9 transcription factor. Pharmacological manipulation of TGFβ1 by PPARγ activators can be applied for treating TGFβ1-induced pathophysiologic disorders such as cancer metastasis and fibrosis. In this article, we will discuss the opposing effects of TGFβ on tumor growth and metastasis, and address the signaling pathways regulated by PPARγ for tumor progression and suppression. (Source: PPAR Research)

Macrophages, ppars, and cancer

PPAR Research — Thu, 03 Jul 2008 11:56:39 +0100

Mononuclear phagocytes often function as control switches of the immune system, securing the balance between pro- and anti-inflammatory reactions. For this purpose and depending on the activating stimuli, these cells can develop into different subsets: proinflammatory classically activated (M1) or anti-inflammatory alternatively activated (M2) macrophages. The expression of the nuclear peroxisome proliferator-activated receptors (PPARs) is regulated by M1- or M2-inducing stimuli, and these receptors are generally considered to counteract inflammatory M1 macrophages, while actively promoting M2 activation. This is of importance in a tumor context, where M1 are important initiators of inflammation-driven cancers. As a consequence, PPAR agonists are potentially usefull for inhibiting the early phases of tumorigenesis through their antagonistic effect on M1. In more established tumors, the macrophage phenotype is more diverse, making it more difficult to predict the outcome of PPAR agonism. Overall, in our view current knowledge provides a sound basis for the clinical evaluation of PPAR ligands as chemopreventive agents in chronic inflammation-associated cancer development, while cautioning against the unthoughtful application of these agents as cancer therapeutics. (Source: PPAR Research)

MedWorm Sponsored Message: Find out how you can get your message across here by sponsoring this MedWorm news feed.

[human skin stem cells]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Human skin stem cells]

J Soc Biol. 2008;202(1):3-6

Authors: Bernard BA

The homeostasis of continuously renewing human epidermis relies on the presence of adult stem cells, residing in the basal layer. Epidermal stem cells have been enriched and functionally characterized, but the exact location remained elusive. The human hair follicle and its pigmentation unit also cyclically regenerate from stem cells. Contrary to epidermal stem cells, human hair follicle stem cells have been localized, enriched, functionally and biochemically characterized. Their specific gene expression pattern has been established. The melanocyte stem population has also been localized and characterized. Finally, the hair follicle was found to harbor a number of other multipotent cells, which designates this unique organ as an alternative source of stem cells for tissue regeneration.

PMID: 18460303 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

[fibroblast subpopulations: a developmental approach of skin physiology and ageing]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Fibroblast subpopulations: a developmental approach of skin physiology and ageing]

J Soc Biol. 2008;202(1):7-14

Authors: Asselineau D, Pageon H, Mine S

Skin is an organ whose function is far beyond a physical barrier between the inside and the outside of the body. Skin as the whole organism is subjected to ageing which concerns skin mostly in its dermal and deepest component which is also its matricial component. The dermis is a tissue rich in matricial elements and poor in cellular content and it is generally admitted that modifications occurring in the matrix are those which mostly contribute to skin ageing, by altering its biomechanical properties. Therefore it is common to address questions related to skin ageing by considering alterations in matrix molecules like collagen. Actually the dermis is a complex tissue both matricial and cellular and is divided between a superficial dermis close to epidermis and a deep dermis much thicker and histologically different. Several years ago we have undertaken investigations related to fibroblasts which are the cells responsible for the formation and maintenance of the dermis, aiming at isolation, culture and characterization of the fibroblasts from the superficial dermis also called papillary dermis and fibroblasts from the deep dermis also called reticular dermis. We were able to show that these fibroblasts in classical culture on plastic exhibit very different morphologies associated with different secretion properties and we have confirmed and expanded such observations revealing different phenotypes by incorporating these cells in reconstructed skin which allows the reproduction of a three-dimensional architecture recalling skin in vivo especially after grafting onto the nude mouse. We also raise the question of how these two dermal regions appear during the formation of the dermis and the question of their fate during ageing. Progress in solving these questions would certainly appear to be very useful for a better understanding of skin physiology and ageing and would hopefully provide new strategies in anti-ageing research.

PMID: 18460304 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

[embryonic stem cells and skin: from cellular model to therapeutic potential]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Embryonic stem cells and skin: from cellular model to therapeutic potential]

J Soc Biol. 2008;202(1):15-23

Authors: Aberdam E, Aberdam D

Embryonic stem (ES) cells are pluripotent cells able to differentiate into many cell types in vitro, thus providing a potential unlimited supply of cells for cognitive in vitro studies and cell-based therapy. We recently reported their efficient ability to recapitulate ectodermal and epidermal fates and form, in culture, a multilayered epidermis coupled with an underlying dermal compartment, similar to native skin. Thus, ES cells have the potential to recapitulate the reciprocal instructive ectodermal-mesodermal commitments, characteristic of embryonic skin formation. We clarified the function of BMP-4 in the binary neuroectodermal choice by stimulating sox-1+ neural precursors to undergo specific apoptosis while inducing epidermal differentiation. We further demonstrated that p63 stimulates ectodermal cell proliferation and is necessary for epidermal commitment. We provided further evidence that this unique cellular model provides a powerful tool to identify the molecular mechanisms controlling normal skin development and to investigate human ectodermal dysplasia congenital pathologies linked to p63 (in p63-ectodermal dysplasia human congenital pathologies). Epidermal stem cell activity has been used for years to repair skin injuries, but ex vivo keratinocyte amplification has limitations and grafted skin homeostasis is not totally satisfactory. Human ES cells raise hopes that the understanding of developmental steps leading to the generation of epidermal stem cells will once be translated into therapeutic benefit. We recently demonstrated that human embryonic stem cells can give rise to a stable somatic ectodermal cell population. Its finite population doubling, normal cell cycle kinetics and the absence of teratoma formation strongly suggest that, although derived from human embryonic stem cells, these ectodermal cells represent a clinically safe somatic cell population. They could thus be particularly useful as a source for committed, homogeneous, non-tumorigenic cell populations to be employed in clinical trials for epithelial stem cell loss.

PMID: 18460305 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

[melanocyte stem cells in adults]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Melanocyte stem cells in adults]

J Soc Biol. 2008;202(1):25-32

Authors: Aubin-Houzelstein G, Djian-Zaouche J, Panthier JJ

Melanocyte stem cells have been recently localized in mice, in the outer root sheath of the lower permanent portion of the hair follicle. Specific depletion of melanocyte stem cell population is responsible for natural hair greying in aging mice and humans. Melanocyte stem cells also seem to drive the growth of malignant melanomas. A few mutations, either spontaneous or genetically engineered, accelerate the natural process of hair greying with age. These mutations allowed the identification of genes and signalling pathways controlling emergence, maintenance and/or differentiation of melanocyte stem cells. This review summarizes recent studies on the melanocyte stem cells and defines a few major unanswered questions in the field.

PMID: 18460306 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

[epidermal stem cells and ex vivo cutaneous gene therapy: application to xeroderma pigmentosum]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Epidermal stem cells and ex vivo cutaneous gene therapy: application to xeroderma pigmentosum]

J Soc Biol. 2008;202(1):33-41

Authors: Warrick E, Bergoglio V, Bernerd F, Magnaldo T

Ex vivo cutaneous gene therapy is an alternative treatment for recessively inherited diseases with cutaneous traits. It relies on the transfer in cultured epidermal keratinocytes of the wild-type allele of the gene whose mutation is responsible for the disease. As for severely burnt patients, epithelial sheets developed from genetically corrected cells may then be grafted back to the patients. Long term correction and graft take depend on the genetic correction of stem cells. Success of such an approach has recently been reported in the case of one patient suffering from a severe case of junctional epidermolysis bullosae. Here we report a method for safely selecting keratinocytes populations after genetic manipulation. The method is non invasive and non immunogenic and allows high enrichment of genetically manipulated stem keratinocytes. This could perhaps contribute to ex vivo gene therapy approaches of cancer prone genodermatoses such as xeroderma pigmentosum.

PMID: 18460307 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

MedWorm Sponsored Message: Find out how you can get your message across here by sponsoring this MedWorm news feed.

[origins and selection of epidermal progenitors and stem cells: a challenge for tissue engineering]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Origins and selection of epidermal progenitors and stem cells: a challenge for tissue engineering]

J Soc Biol. 2008;202(1):43-54

Authors: Deshayes N, Rathman-Josserand M

The use of epidermal stem cells and their progeny for tissue engineering and cell therapy represents a source of hope and major interest in view of applications such as replacing the loss of functionality in failing tissues or obtaining physiologic skin equivalents for skin grafting. The use of such cells necessitates the isolation and purification of rare populations of keratinocytes and then increasing their numbers by mass culture. This is not currently possible since part of the specific phenotype of these cells is lost once the cells are placed in culture. Furthermore, few techniques are available to unequivocally detect the presence of skin stem cells and/or their progeny in culture and thus quantify them. Two different sources of stem cells are currently being studied for skin research and clinical applications: skin progenitors either obtained from embryonic stem cells (ESC) or from selection from adult skin tissue. It has been shown that "keratinocyte-like" cells can be derived from ESC; however, the culturing processes must still be optimized to allow for the mass culture of homogeneous populations at a controlled stage of differentiation. The functional characterization of such populations must also be more thoroughly achieved. In order to use stem cells from adult tissues, improvements must be made in order to obtain a satisfactory degree of purification and characterization of this rare population. Distinguishing stem cells from progenitor cells at the molecular level also remains a challenge. Furthermore, stem cell research inevitably requires cultivating these cells outside their physiological environment or niche. It will thus be necessary to better understand the impact of this specific environmental niche on the preservation of the cellular phenotypes of interest.

PMID: 18460308 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

[stem cells from human interfollicular epidermis: phenotypes and potentialities]

Journal de la Societe de Biologie — Thu, 03 Jul 2008 11:39:25 +0100

[Stem cells from human interfollicular epidermis: phenotypes and potentialities]

J Soc Biol. 2008;202(1):55-65

Authors: Fortunel NO, Martin MT

Stem cells from different tissue origins share common characteristics, including selfrenewal capacity and tissue regeneration potential. Finding criteria to identify particular stem cell types, and understanding signaling pathways responsible for stemness, represent major research areas that will lead to a better characterization of the normal state of stem cells, thus improving our capability to use them for regenerative therapies. We will review here different approaches and experimental models liable to increase our knowledge of stem cells from human interfollicular epidermis. One of them, based on transcriptional profiling performed at the level of the global genome, consisted in searching universal molecular markers of stem cells. In other approaches, stem cells were studied at the level of specific characteristics. Understanding somatic stem cell properties such as quiescence or slow cycling state, and detoxification potential, led to the identification of phenotypes suitable for the selection of epidermal keratinocyte sub-populations with stem cell properties. The specific interests of these different research strategies will be discussed.

PMID: 18460309 [PubMed - in process]

(Source: Journal de la Societe de Biologie)

Red wine ingredient wards off effects of age on heart, bones, eyes and muscle