MedWorm provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest medical blog items that have been tagged with 'immunohistochemistry'. Sat, 03 Sep 2011 02:45:22 +0100 http://www.medworm.com/index.php?rid=4406044&cid=t_262072_155_f&fid=38409&url=http%3A%2F%2Fneuropathologyblog.blogspot.com%2F2011%2F01%2Fbest-post-of-august-10-highly-pure-anti.html August 2010 is next up in our "Best of the Month" series. The following guest post from Dr. Olaf Bartsch appeared on August 27th and addresses an important development in the world of neuropathological immunohistochemistry. (Note: I'm not sure whether free samples are still available.)The German company Dianova now provides reliable highly pure (goat anti-mouse chromatography) lyophilizates of antibody clone H09. The antibody has been purified from cell culture supernatants by GAM (goat anti-mouse) affinity chromatography resulting in a more intense staining of oligodendroglioma versus a clear negative reaction with glioblastoma.Dianova's proprietary antibodies are now available in the USA directly and can be ordered from U.S. and Canadian customers on the HistoBioTec website or by purchas... neuropathology blog blogs http://www.medworm.com/rss/comments.php?id=4406044 Wed, 26 Jan 2011 19:56:00 +0100 4406044 http://www.medworm.com/index.php?rid=4122094&cid=t_262072_155_f&fid=38409&url=http%3A%2F%2Fneuropathologyblog.blogspot.com%2F2010%2F10%2Fbest-post-of-may-2010-free-samples-of.html The next in our Best of the Month series is from May 13, 2010. Free samples of IDH1 may or may not still be available, but you can check with the company:Dianova, the German company that makes anti-human IDH1 antibody for use in immunohistochemical staining of gliomas versus gliosis, is providing free samples of its product. Just go to the site, click on "free sample", and make a request via email. This marker may well be a tremendous advancement in distinguishing reactive astrocytosis from diffuse astrocytoma, so I would encourage you to get your free sample! Thanks to Drs. Craig Horbinski and Ty Abel for assisting me in discovering this opportunity. (Source: neuropathology blog) neuropathology blog blogs http://www.medworm.com/rss/comments.php?id=4122094 Sun, 31 Oct 2010 15:48:00 +0100 4122094 http://www.medworm.com/index.php?rid=4017874&cid=t_262072_139_f&fid=38879&url=http%3A%2F%2Ffeedproxy.google.com%2F%7Er%2FVirologyBlog%2F%7E3%2FXOYZJusvX68%2F Many virological techniques are based on the specificity of the antibody-antigen reaction. Examples in our virology toolbox include western blot analysis and ELISA. While very useful, these methods cannot be used to visualize viral proteins in infected cells or tissues. To do that we must turn to immunostaining. In direct immunostaining (illustrated), an antibody that recognizes a viral antigen is coupled directly to an indicator (a fluorescent dye or an enzyme). Indirect immunostaining is a more sensitive method because a second antibody is coupled to the indicator. The second antibody recognizes a common epitope on the virus-specific antibody. Multiple second antibodies can bind to the first antibody, leading to an increased signal from the indicator compared to direct immunostaining. To... virology blog blogs http://www.medworm.com/rss/comments.php?id=4017874 Thu, 30 Sep 2010 15:06:04 +0100 4017874 http://www.medworm.com/index.php?rid=3911878&cid=t_262072_155_f&fid=38409&url=http%3A%2F%2Fneuropathologyblog.blogspot.com%2F2010%2F08%2Fhighly-pure-anti-idh1-r132h.html A guest post regarding an important development from Dr. Olaf Bartsch:The German company Dianova now provides reliable highly pure (goat anti-mouse chromatography) lyophilizates of antibody clone H09. The antibody has been purified from cellculture supernatants by GAM (goat anti-mouse) affinity chromatography resulting in a more intense staining of oligodendroglioma versus a clear negative reaction with glioblastoma.Dianova's proprietary antibodies are now available in the USA directly and can be ordered from U.S. and Canadian customers on the HistoBioTec website or by purchase order.Please see the datasheet for details and order a free sample. Fig.1Double staining of GFAP (red) and clone H09 (brown) of oligodendrogliopma infiltrating zone demonstrating specific labelling of tumor cells ... neuropathology blog blogs http://www.medworm.com/rss/comments.php?id=3911878 Fri, 27 Aug 2010 20:48:00 +0100 3911878 http://www.medworm.com/index.php?rid=3490830&cid=t_262072_136_f&fid=37846&url=http%3A%2F%2Fhealthinfoispower.wordpress.com%2F2010%2F04%2F20%2Fexpression-of-proteins-linked-to-poor-outcome-in-women-with-ovarian-cancer%2F Scientists have established the presence of certain proteins in ovarian cancer tissues and have linked these proteins to poor survival rates in women with advanced stages of the disease. Scientists have established the presence of certain proteins in ovarian cancer tissues and have linked these proteins to poor survival rates in women with advanced [...] (Source: Libby's H*O*P*E*) Libby's H*O*P*E* blogs http://www.medworm.com/rss/comments.php?id=3490830 Wed, 21 Apr 2010 00:09:14 +0100 3490830 http://www.medworm.com/index.php?rid=3416373&cid=t_262072_155_f&fid=38410&url=http%3A%2F%2Ffeedproxy.google.com%2F%7Er%2FOncopathology%2F%7E3%2FYImc7GKKYrE%2Fimportance-of-immunohistochemistry-in.html Before discussing the utility of particular immunostains, it is important to review and understand some basics about the normal lymphoid follicle, in order to interpret the immunostains appropriately.The difference between a primary follicle and a secondary follicle:A primary or "resting" follicle: - a collection of B-cells in the cortex of the lymph node that has not been antigenically stimulated, and because of this, it does not have a germinal center.A secondary follicle:- Once a primary follicle has been antigenically simulated, it acquires a germinal center, and at this point is referred to as secondary follicle.Differential diagnosis of lymphoid lesion with follicular structures or nodules, :-1) Resting (primary) follicles,2) Secondary follicles (as a reflection of reactive follicula... Oncopathology blogs http://www.medworm.com/rss/comments.php?id=3416373 Mon, 06 Apr 2009 12:58:00 +0100 3416373