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Swine Flu

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Genetic Characterization and Evolution of H1N1pdm09 after Circulation in a Swine Farm.
In this study we investigated the molecular evolution of A(H1N1)pdm09 virus identified in pigs reared in a single herd. Nasal swabs taken from pigs showing respiratory distress were tested for influenza type A and A(H1N1)pdm09 by real-time RT-PCR assays. Virus isolation from positive samples was attempted by inoculation of nasal swabs samples into specific pathogen free embryonated chicken eggs (ECE) and complete genome sequencing was performed on virus strains after replication on ECE or from original swab sample. The molecular analysis of hemagglutinin (HA) showed, in four of the swine influenza viruses under study, a un...
Source: Biomed Res - July 19, 2014 Category: Research Authors: Boni A, Vaccari G, Di Trani L, Zaccaria G, Alborali GL, Lelli D, Cordioli P, Moreno AM Tags: Biomed Res Int Source Type: research

New analysis of 'swine flu' pandemic conflicts with accepted views on how diseases spread
(University of Cambridge) New analysis of the 2009 H1N1 influenza pandemic in the US shows that the pandemic wave was surprisingly slow, and that its spread was likely accelerated by school-age children. (Source: EurekAlert! - Infectious and Emerging Diseases)
Source: EurekAlert! - Infectious and Emerging Diseases - July 1, 2014 Category: Infectious Diseases Source Type: news

Healthcare workers under a mandated H1N1 vaccination policy with employment termination penalty: A survey to assess employee perception.
Abstract The ethical debate over mandatory healthcare worker (HCW) influenza vaccination is a heated one. Our study hospital instituted a mandatory employee influenza vaccination policy for the 2009-2010 influenza season during the highly publicized pandemic of the H1N1 "Swine Flu." Under this mandate there was no informed declination option, and termination of employment was the consequence for noncompliance. Our objective was to examine HCW perceptions of the H1N1 influenza virus, the vaccine, and the strict mandated vaccination policy. A survey was designed, distributed, and anonymously collected. In total, 202 ...
Source: Vaccine - July 1, 2014 Category: Allergy & Immunology Authors: Winston L, Wagner S, Chan S Tags: Vaccine Source Type: research

Utility of snout wipe samples for influenza A virus surveillance in exhibition swine populations
ConclusionsViral recoveries from potential snout wipe materials ranged from 0·26 to 1·59 log10 TCID50/ml less than that of the positive control in which no substrate was included; rayon/polyester gauze performed significantly worse than the other materials. In the field, snout wipes and nasal swabs had high levels of agreement for both rRT‐PCR detection and virus isolation. Although further investigation and refinement of the sampling method is needed, results indicate that snout wipes will facilitate convenient and undisruptive IAV surveillance in pigs at agricultural fairs. (Source: Influenza and Other Respiratory Viruses)
Source: Influenza and Other Respiratory Viruses - July 1, 2014 Category: Infectious Diseases Authors: Jody L. Edwards, Sarah W. Nelson, Jeffrey D. Workman, Richard D. Slemons, Christine M. Szablewski, Jacqueline M. Nolting, Andrew S. Bowman Tags: Original Article Source Type: research

A flow-through chromatography process for influenza A and B virus purification.
This study focused on the development of an economic flow-through chromatographic process avoiding virus strain sensitive capture steps. Therefore, a three-step process consisting of anion exchange chromatography (AEC), Benzonase(®) treatment, and size exclusion chromatography with a ligand-activated core (LCC) was established, and tested for purification of two influenza A virus strains and one influenza B virus strain. The process resulted in high virus yields (≥68%) with protein contamination levels fulfilling requirements of the European Pharmacopeia for production of influenza vaccines for human use. DNA was deplet...
Source: Journal of Virological Methods - July 1, 2014 Category: Virology Authors: Weigel T, Solomaier T, Peuker A, Pathapati T, Wolff MW, Reichl U Tags: J Virol Methods Source Type: research

N-acetyl-l-cystine (NAC) protects against H9N2 swine influenza virus-induced acute lung injury.
Abstract The antioxidant N-acetyl-l-cysteine (NAC) had been shown to inhibit replication of seasonal human influenza A viruses. Here, the effects of NAC on H9N2 swine influenza virus-induced acute lung injury (ALI) were investigated in mice. BALB/c mice were inoculated intranasally with 10(7) 50% tissue culture infective doses (TCID50) of A/swine/HeBei/012/2008/(H9N2) viruses with or without NAC treatments to induce ALI model. The result showed that pulmonary inflammation, pulmonary edema, MPO activity, total cells, neutrophils, macrophages, TNF-α, IL-6, IL-1β and CXCL-10 in BALF were attenuated by NAC. Moreover,...
Source: International Immunopharmacology - June 23, 2014 Category: Allergy & Immunology Authors: Zhang RH, Li CH, Wang CL, Xu MJ, Tong X, Dong W, Liu BJ, Wang GH, Tian SF Tags: Int Immunopharmacol Source Type: research

Immunotherapies influence the influenza vaccination response in multiple sclerosis patients: an explorative study
Conclusions: These findings suggest that MS patients receiving immunomodulatory therapies other than interferon beta should be considered for a vaccine response analysis and perhaps be offered a second dose of the vaccine, in cases of insufficient protection. (Source: Multiple Sclerosis)
Source: Multiple Sclerosis - June 16, 2014 Category: Neurology Authors: Olberg, H. K., Cox, R. J., Nostbakken, J. K., Aarseth, J. H., Vedeler, C. A., Myhr, K.-M. Tags: Research Papers Source Type: research

Reassortant swine influenza viruses isolated in Japan contain genes from pandemic A(H1N1) 2009
ABSTRACT In 2013, three reassortant swine influenza viruses (SIVs)—two H1N2 and one H3N2—were isolated from symptomatic pigs in Japan; each contained genes from the pandemic A(H1N1) 2009 virus and endemic SIVs. Phylogenetic analysis revealed that the two H1N2 viruses, A/swine/Gunma/1/2013 and A/swine/Ibaraki/1/2013, were reassortants that contain genes from the following three distinct lineages: (i) H1 and nucleoprotein (NP) genes derived from a classical swine H1 HA lineage uniquely circulating among Japanese SIVs; (ii) neuraminidase (NA) genes from human‐like H1N2 swine viruses; and (iii) other genes from pandemic ...
Source: Microbiology and Immunology - June 9, 2014 Category: Microbiology Authors: Katsushi Kanehira, Nobuhiro Takemae, Yuko Uchida, Hirokazu Hikono, Takehiko Saito Tags: Original Article Source Type: research

MicroRNA Expression in Mice Infected with Seasonal H1N1, Swine H1N1, or Highly Pathogenic H5N1.
In this study, we demonstrate differences in the miRNA expression profiles associated with the lung in mice infected with various influenza viruses that vary in virulence and pathogenicity. Additionally, this study employs a statistical model that utilizes changes in miRNA expression to identify the virus that was used to infect the mice. In all, this project identifies a unique fingerprint of viral pathogenicity associated with seasonal H1N1, swine H1N1, and highly pathogenic H5N1 in the mouse model and may lead to the identification of novel therapeutic and prophylactic targets. PMID: 24913561 [PubMed - as supplied b...
Source: Journal of Medical Microbiology - June 9, 2014 Category: Microbiology Authors: Vela EM, Kasoji MD, Wendling MQ, Price JA, Knostman KA, Bresler HS, Long JP Tags: J Med Microbiol Source Type: research

RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR
Real-time RT-PCR (rRT-PCR) assays are currently the method of choice in many laboratories for the detection and subtyping of influenza A virus (IAV) in swine. Traditionally, nasal swabs and lung tissues (sometimes broncho-alveolar lavage and tracheal tissues) are the primary specimens for IAV testing. However, oral fluids are becoming more common for IAV prognostic profiling. In this chapter, we describe (1) procedures of RNA extraction from the common clinical specimens, (2) two rRT-PCR assays for detection of IAV in swine, and (3) an rRT-PCR assay for subtyping swine IAV. RNA extraction procedures include a magnetic bead...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Hemagglutinin Inhibition Assay with Swine Sera
Hemagglutination is based on the ability of viruses such as influenza A virus to agglutinate red blood cells (RBCs) of specific animal species by formation of cross-linking lattices between RBCs. Antibodies that have the ability to inhibit the hemagglutination property of influenza A viruses are correlated with protection from infection. The hemagglutination inhibition (HI) test is a serological assay that measures the titer of specific antibodies in the sera and is the most common serological assay used to detect anti-influenza antibodies in swine sera. (Source: Springer protocols feed by Infectious Diseases)
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype-Specific IgG and IgA Whole-Virus Antibody to Influenza A Virus in Swine
Enzyme-linked immunosorbent assays can be used to detect isotype-specific anti-influenza antibodies in biological samples to characterize the porcine immune response to influenza A virus. The isotype antibody assay is based on an indirect ELISA using whole influenza virus as antigen and detection antibodies directed against porcine IgG and IgA. Samples such as serum, nasal wash, and bronchoalveolar lavage fluid allow for evaluation of systemic, upper, and lower respiratory tract mucosal antibody responses, respectively. The isotype ELISA assay is performed in a 96-well format using anti-swine detection antibodies conjugate...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

A Brief Introduction to Influenza A Virus in Swine
Influenza A viruses (IAV) of the Orthomyxoviridae virus family cause one of the most important respiratory diseases in pigs as well as humans. Repeated outbreaks and rapid spread of genetically and antigenically distinct IAVs represent a considerable challenge for animal production and public health. This overlap between human and animal health is a prime example of the “One Health” concept. Although only subtypes of H1N1, H1N2, and H3N2 are endemic in swine around the world, considerable diversity can be found not only in the hemagglutinin (HA) and neuraminidase (NA) genes, but in the other 6 genes as well. Hu...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Sample Types, Collection, and Transport for Influenza A Viruses of Swine
Detection of influenza A virus (IAV), viral antigen, nucleic acid, or antibodies in swine is dependent upon the collection of the appropriate sample type, the quality of the sample, and the proper storage and handling of the sample. The diagnostic tests to be performed should be considered prior to sample collection. Sera are acceptable samples for ELISA or hemagglutination inhibition tests, but not for real-time RT-PCR. Likewise, swabs and/or tissues are acceptable for real-time RT-PCR and virus isolation. The sample type will also depend on the age of swine that are being tested; oral fluids can be successfully collected...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs
Influenza virus isolation is a procedure to obtain a live and infectious virus that can be used for antigenic characterization, pathogenesis investigation, and vaccine production. Embryonated chicken egg inoculation is traditionally considered the “gold standard” method for influenza virus isolation and propagation. However, many primary cells and continuous cell lines have also been examined or developed for influenza virus isolation and replication. Specifically, swine influenza virus (SIV) isolation and propagation have been attempted and compared in embryonated chicken eggs, some primary porcine cells, and ...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs
Avian influenza virus and some mammalian influenza A viruses are usually isolated, propagated, and titrated in embryonated chicken eggs (ECE). Most any sample type can be accommodated for culture with appropriate processing. Isolation may also be accomplished in cell culture particularly if mammalian lineage isolates are suspected, for example, swine influenza in turkey specimens. Culture is highly sensitive, but is not specific for influenza A, which may be an advantage because a sample may be screened for several agents at once. Once an agent is isolated in culture, the presence of influenza viruses is confirmed with any...
Source: Springer protocols feed by Infectious Diseases - June 6, 2014 Category: Infectious Diseases Source Type: news

Research/Review: Structure and Linkage Disequilibrium Analysis of Adamantane Resistant Mutations in Influenza Virus M2 Proton Channel.
Abstract The M2 proton channel is translated by the M gene segment of influenza viruses, and has been adopted as an attractive target for influenza A viruses, on which a series of adamantane-based drugs act. However, recently epidemic influenza viruses have strong resistant effects against the adamantane-based drugs. In this paper, we combined evolutionary analyses, linkage disequilibrium as well as molecular dynamics simulations to explore the drug resistance of the M2 proton channel, with an aim of providing an in-depth understanding of the resistant mechanism for adamantane-based drugs. We collected 2746 coding ...
Source: Current Drug Metabolism - June 5, 2014 Category: Drugs & Pharmacology Authors: Yu Y, Li X, Hao P, Wang JF, Chou KC Tags: Curr Drug Metab Source Type: research

The influence of experimental infection of gilts with swine H1N2 influenza A virus during the second month of gestation on the course of pregnancy, reproduction parameters and clinical status
Conclusions: The results of the study indicate that infection of pregnant gilts with swine H1N2 IAV in the second month of pregnancy does not cause abortion and other reproduction disorders. No evidence for transplacental transmission of swine H1N2 IAV was found. However, due to subclinical course of influenza in the present experiment caution should be taken in extrapolating these results to the cases of acute influenza. The other limitation is IAV diversity. It cannot be excluded that other subtypes of IAV could be associated to reproduction failure in pigs. (Source: BioMed Central)
Source: BioMed Central - June 4, 2014 Category: Journals (General) Authors: Krzysztof KwitMa¿gorzata Pomorska-MólIwona Markowska-Daniel Source Type: research

Origins and pathogenesis of the 1918 flu pandemic [Evolution]
The source, timing, and geographical origin of the 1918–1920 pandemic influenza A virus have remained tenaciously obscure for nearly a century, as have the reasons for its unusual severity among young adults. Here, we reconstruct the origins of the pandemic virus and the classic swine influenza and (postpandemic) seasonal H1N1... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - June 3, 2014 Category: Science Authors: Worobey, M., Han, G.-Z., Rambaut, A. Tags: Biological Sciences Source Type: research

Cytokine Expression at Different Stages of Influenza A(H1N1)pdm09 Virus Infection in the Porcine Lung, Using Laser Capture Microdissection
This study highlighted the value of using laser capture microdissection to isolate specific tissue regions and investigate subtle differences in cytokine mRNA expression during lesion development in pigs infected with influenza A(H1N1)pdm09. (Source: Transboundary and Emerging Diseases)
Source: Transboundary and Emerging Diseases - June 1, 2014 Category: Veterinary Research Authors: D. J. Hicks, M. Kelly, S. M. Brookes, B. Z. Londt, A. Ortiz Pelaez, A. Orlowska, I. H. Brown, Y. I. Spencer, A. Núñez Tags: Original Article Source Type: research

Variant Influenza Associated with Live Animal Markets, Minnesota
Summary Variant influenza viruses are swine‐origin influenza A viruses that cause illness in humans. Surveillance for variant influenza A viruses, including characterization of exposure settings, is important because of the potential emergence of novel influenza viruses with pandemic potential. In Minnesota, we have documented variant influenza A virus infections associated with swine exposure at live animal markets. (Source: Zoonoses and Public Health)
Source: Zoonoses and Public Health - June 1, 2014 Category: Infectious Diseases Authors: M. J. Choi, C. A. Morin, J. Scheftel, S. M. Vetter, K. Smith, R. Lynfield, Tags: Short Communication Source Type: research

Avian-Like A (H1N1) Swine Influenza Virus Antibodies among Swine Farm Residents and Pigs in Southern China.
Abstract Infection of human with avian-like A (H1N1) swine influenza virus (SIV) occasionally occurs in China, suggesting a potential risk of cross-species transmission of the swine influenza H1N1 virus from pigs to humans, particularly to those having direct contact with pigs. A seroepidemiological study was conducted to assess the prevalence of antibodies against the avian-like A (H1N1) SIV among swine farm residents and pigs in southern China to evaluate the risk of infection to swine farm workers. Hemagglutination inhibition (HI) assays revealed that 11.17% (61/546) of the sera samples from swine farm residents...
Source: Japanese Journal of Infectious Diseases - May 29, 2014 Category: Infectious Diseases Authors: Zhou H, Cao Z, Tan L, Fu X, Lu G, Qi W, Ke C, Wang H, Sun L, Zhang G Tags: Jpn J Infect Dis Source Type: research

Influenza at the animal-human interface: a review of the literature for virological evidence of human infection with swine or avian influenza viruses other than A(H5N1).
PMID: 24832117 [PubMed - in process] (Source: Euro Surveill)
Source: Euro Surveill - May 19, 2014 Category: Infectious Diseases Authors: Freidl G, Meijer A, de Bruin E, de Nardi M, Munoz O, Capua I, Breed A, Harris K, Hill A, Kosmider R, Banks J, von Dobschuetz S, Stark K, Wieland B, Stevens K, van der Werf S, Enouf V, van der Meulen K, Van Reeth K, Dauphin G, Koopmans M, FLURISK Consortiu Tags: Euro Surveill Source Type: research

Tamiflu: drugs given for swine flu 'were waste of £500m'
Drug Tamiflu does nothing to halt the spread of influenza and Government wasted nearly £500?million stockpiling it over swine flu pandemic, study finds (Source: The Telegraph : Swine Flu A H1N1)
Source: The Telegraph : Swine Flu A H1N1 - May 13, 2014 Category: Infectious Diseases Tags: influenza drugs pandemic tamiflu swine flu Source Type: news

Vaccinia‐based influenza vaccine overcomes previously induced immunodominance hierarchy for heterosubtypic protection
This article is protected by copyright. All rights reserved (Source: European Journal of Immunology)
Source: European Journal of Immunology - May 13, 2014 Category: Allergy & Immunology Authors: Ji‐Sun Kwon, Jungsoon Yoon, Yeon‐Jung Kim, Kyuho Kang, Sunje Woo, Dea‐Im Jung, Man Ki Song, Eun‐Ha Kim, Hyeok‐il Kwon, Young Ki Choi, Jihye Kim, Jeewon Lee, Yeup Yoon, Eui‐Cheol Shin, Jin‐Won Youn Tags: Regular Article Source Type: research

Vaccinia-based influenza vaccine overcomes previously induced immunodominance hierarchy for heterosubtypic protection.
This article is protected by copyright. All rights reserved. PMID: 24825439 [PubMed - as supplied by publisher] (Source: European Journal of Immunology)
Source: European Journal of Immunology - May 13, 2014 Category: Allergy & Immunology Authors: Kwon JS, Yoon J, Kim YJ, Kang K, Woo S, Jung DI, Song MK, Kim EH, Kwon HI, Choi YK, Kim J, Lee J, Yoon Y, Shin EC, Youn JW Tags: Eur J Immunol Source Type: research

Protective efficacy of intranasally administered bivalent live influenza vaccine and immunological mechanisms underlying the protection.
Abstract Previously we reported the generation of a new potential live attenuated influenza vaccine (LAIV) named SIV/606 that expresses H1 and H3 HAs. We also demonstrated intratracheal vaccination of SIV/606 conferred protection against infections with both H1 and H3 swine influenza virus subtypes in pigs. Here we vaccinated pigs with SIV/606 intranasally, which is a more suitable route for LAIV, and evaluated vaccine efficacy. Intranasal vaccination of SIV/606 induced serum IgG antibody responses against both H1N1 and H3N2 SIVs and high titer of virus neutralizing antibodies against H1N1 SIV but not against H3N2 ...
Source: Vaccine - May 13, 2014 Category: Allergy & Immunology Authors: Pyo HM, Zhou Y Tags: Vaccine Source Type: research

Influenza at the animal-human interface: a review of the literature for virological evidence of human infection with swine or avian influenza viruses other than A(H5N1)
(Source: Eurosurveillance latest news)
Source: Eurosurveillance latest news - May 8, 2014 Category: Infectious Diseases Source Type: research

Epistatically Interacting Substitutions Are Enriched during Adaptive Protein Evolution
by Lizhi Ian Gong, Jesse D. Bloom Most experimental studies of epistasis in evolution have focused on adaptive changes—but adaptation accounts for only a portion of total evolutionary change. Are the patterns of epistasis during adaptation representative of evolution more broadly? We address this question by examining a pair of protein homologs, of which only one is subject to a well-defined pressure for adaptive change. Specifically, we compare the nucleoproteins from human and swine influenza. Human influenza is under continual selection to evade recognition by acquired immune memory, while swine influenza experiences...
Source: PLoS Genetics - May 8, 2014 Category: Genetics & Stem Cells Authors: Lizhi Ian Gong et al. Source Type: research

Solving the Mystery Flu That Killed 50 Million People
MoreCalifornia Bill Banning ‘Affluenza’ Defense Is Nixed7 Ebola Patients in Guinea Fight Off the Disease4 Diseases Making a Comeback Thanks to Anti-VaxxersYears ago the environmental historian Alfred Crosby was at Washington State University, where he was teaching at the time, when on a whim he decided to pick up an old almanac from 1917. (This is apparently the kind of thing historians like to do in their spare time.) He looked up the U.S. life expectancy in that year—it was about 51 years. He turned to the 1919 almanac, and found about the same figure. Then Crosby picked up the almanac from 1918. The U.S. l...
Source: TIME: Top Science and Health Stories - April 29, 2014 Category: Science Authors: Bryan Walsh Tags: Uncategorized 1918 pandemic avian flu bird flu death rates H1N1 H5N1 H7N9 health immune system influenza Spanish flu Source Type: news

Reassortant swine influenza viruses isolated in Japan contain genes from pandemic A(H1N1)2009
Abstract In 2013, three ressortant swine influenza viruses (SIVs)—two H1N2 and one H3N2—were isolated from symptomatic pigs in Japan; each contained genes from the pandemic A(H1N1)2009 virus and endemic SIVs. Phylogenetic analysis revealed that the two H1N2 viruses, A/swine/Gunma/1/2013 and A/swine/Ibaraki/1/2013, were reassortants that contain genes from three distinct lineages: 1) H1 and NP genes derived from a classical swine H1 HA lineage uniquely circulating among Japanese SIVs; 2) NA genes from human‐like H1N2 swine viruses; and 3) other genes from pandemic A(H1N1)2009 viruses. The H3N2 virus, A/swine/Miyazaki/...
Source: Microbiology and Immunology - April 17, 2014 Category: Microbiology Authors: Katsushi Kanehira, Nobuhiro Takemae, Yuko Uchida, Hirokazu Hikono, Takehiko Saito Tags: Original Article Source Type: research

The origin of the PB1 segment of swine influenza A virus subtype H1N2 determines viral pathogenicity in mice.
Abstract Swine appear to be a key species in the generation of novel human influenza pandemics. Previous pandemic viruses are postulated to have evolved in swine by reassortment of avian, human, and swine influenza viruses. The human pandemic influenza viruses that emerged in 1957 and 1968 as well as swine viruses circulating since 1998 encode PB1 segments derived from avian influenza viruses. Here we investigate the possible role in viral replication and virulence of the PB1 gene segments present in two swine H1N2 influenza A viruses, A/swine/Sweden/1021/2009(H1N2) (sw 1021) and A/swine/Sweden/9706/2010(H1N2) (sw ...
Source: Virus Research - April 12, 2014 Category: Virology Authors: Metreveli G, Gao Q, Mena N, Schmolke M, Berg M, Albrecht RA, García-Sastre A Tags: Virus Res Source Type: research

Assessment of seasonality of influenza in swine using field submissions to a diagnostic laboratory in Ontario between 2007 and 2012
ConclusionsThus, this study identified evidence of seasonality in influenza‐like disease in swine herds, but not in circulation of influenza virus. Evidence of seasonality in exposure to influenza was dependent on assumptions of between‐month correlation. High exposure to H3N2 and H1N1 subtypes warrants more detailed investigation of within‐herd influenza virus circulation. The study provides initial insight into seasonality of influenza in swine and should be followed with herd‐level studies. (Source: Influenza and Other Respiratory Viruses)
Source: Influenza and Other Respiratory Viruses - April 11, 2014 Category: Infectious Diseases Authors: Zvonimir Poljak, Susy Carman, Beverly McEwen Tags: Original Article Source Type: research

Effectiveness of Tamiflu and Relenza questioned
ConclusionThis major review is particularly significant for its use of unpublished, previously confidential data from both the drug manufacturers and regulators, to verify the information in published trials. As the researchers point out, much of the trial data is unreliable for various reasons, which makes it difficult to draw firm conclusions.While it appears that these drugs have a modest benefit, there is no solid evidence that either drug can protect people from the more serious complications of influenza. Paracetamol or ibuprofen would seem to be a far more cost-effective method of relieving the symptoms of influenza...
Source: NHS News Feed - April 10, 2014 Category: Consumer Health News Tags: Medication Swine flu Source Type: news

Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices
Viral respiratory diseases remain of major importance in swine breeding units. Swine influenza virus (SIV) is one of the main known contributors to infectious respiratory diseases. The innate immune response to swine influenza viruses has been assessed in many previous studies. However most of these studies were carried out in a single-cell population or directly in the live animal, in all its complexity. In the current study we report the use of a trachea epithelial cell line (newborn pig trachea cells - NPTr) in comparison with alveolar macrophages and lung slices for the characterization of innate immune response to an ...
Source: BioMed Central - April 9, 2014 Category: Journals (General) Authors: Mario Delgado-OrtegaSandrine MeloDarsaniya PunyadarsaniyaChristelle RaméMichel OlivierDenis SoubieuxDaniel MarcGaëlle SimonGeorg HerrlerMustapha BerriJoëlle DupontFrançois Meurens Source Type: research

Transcriptional approach to study porcine tracheal epithelial cells individually or dually infected with swine influenza virus and Streptococcus suis
Conclusion: Influenza virus would replicate in the respiratory epithelium and induce an inflammatory infiltrate comprised of mononuclear cells and neutrophils. In a co-infection situation, although these cells would be unable to phagocyte and kill S. suis, they are highly activated by this pathogen. S. suis is not considered a primary pulmonary pathogen, but an exacerbated production of proinflammatory mediators during a co-infection with influenza virus may be important in the pathogenesis and clinical outcome of S. suis-induced respiratory diseases. (Source: BMC Veterinary Research - Latest articles)
Source: BMC Veterinary Research - Latest articles - April 7, 2014 Category: Veterinary Research Authors: Yuan DangClaude LachanceYingchao WangCarl GagnonChristian SavardMariela SeguraDaniel GrenierMarcelo Gottschalk Source Type: research

The adjuvant component α-tocopherol triggers via modulation of Nrf2 the expression and turnover of hypocretin in vitro and its implication to the development of narcolepsy.
CONCLUSION: In case of a genetic predisposition (DQB1*602) α-tocopherol could confer to development of narcolepsy by activation of Nrf2 that finally leads to an elevated formation of longer hypocretin-derived fragments that can be presented by HLA-subtype DQB1*602. These cells are recognized by the immune system and due to their increased sensitivity to apoptotic stimuli they can be destroyed, finally leading to a lack of hypocretin. PMID: 24721530 [PubMed - as supplied by publisher] (Source: Vaccine)
Source: Vaccine - April 7, 2014 Category: Allergy & Immunology Authors: Masoudi S, Ploen D, Kunz K, Hildt E Tags: Vaccine Source Type: research

Immunogenicity and efficacy of a recombinant adenovirus expressing hemagglutinin from the H5N1 subtype of swine influenza virus in mice.
In this study we generated a replication-defective recombinant adenovirus (rAd-H5HA-EGFP) expressing the hemagglutinin (HA) gene of H5N1 A/Swine/Fujian/1/2001 (SW/FJ/1/01) and evaluated its immunogenicity and protective efficacy in BALB/c mice. The recombinant virus induced high levels of hemagglutination inhibition (HI) antibody at a median tissue culture infective dose of 10(8) or 10(7). Compared with mice in the control groups, the mice vaccinated with rAd-H5HA-EGFP did not show apparent weight loss after challenge with either the homologous SW/FJ/1/01 or the heterologous H5N1 A/Chicken/Hunan/77/2005 (CK/HuN/77/05). Rep...
Source: Canadian Journal of Veterinary Research - April 1, 2014 Category: Veterinary Research Authors: Wu Y, Qiao C, Yang H, Chen Y, Xin X, Chen H Tags: Can J Vet Res Source Type: research

Intranasally administered Endocine™ formulated 2009 pandemic influenza H1N1 vaccine induces broad specific antibody responses and confers protection in ferrets.
Abstract Influenza is a contagious respiratory disease caused by an influenza virus. Due to continuous antigenic drift of seasonal influenza viruses, influenza vaccines need to be adjusted before every influenza season. This allows annual vaccination with multivalent seasonal influenza vaccines, recommended especially for high-risk groups. There is a need for a seasonal influenza vaccine that induces broader and longer lasting protection upon easy administration. Endocine™ is a lipid-based mucosal adjuvant composed of endogenous lipids found ubiquitously in the human body. Intranasal administration of influenza a...
Source: Vaccine - March 29, 2014 Category: Allergy & Immunology Authors: Maltais AK, Stittelaar KJ, Kroeze EJ, van Amerongen G, Dijkshoorn ML, Krestin GP, Hinkula J, Arwidsson H, Lindberg A, Osterhaus AD Tags: Vaccine Source Type: research

One‐Health Simulation Modelling: A Case Study of Influenza Spread between Human and Swine Populations using NAADSM
Summary The circulation of zoonotic influenza A viruses including pH1N1 2009 and H5N1 continue to present a constant threat to animal and human populations. Recently, an H3N2 variant spread from pigs to humans and between humans in limited numbers. Accordingly, this research investigated a range of scenarios of the transmission dynamics of pH1N1 2009 virus at the swine–human interface while accounting for different percentages of swine workers initially immune. Furthermore, the feasibility of using NAADSM (North American Animal Disease Spread Model) applied as a one‐health simulation model was assessed. The study popul...
Source: Transboundary and Emerging Diseases - March 24, 2014 Category: Veterinary Research Authors: S. Dorjee, C. W. Revie, Z. Poljak, W. B. McNab, J. Sanchez Tags: Original Article Source Type: research

Three-quarters of people with flu have no symptoms
ConclusionThis large, community-based study found that most people with influenza in England don’t have symptoms, and even if they do, only a small proportion go to a doctor.Approximately 20% of people had an increase in antibodies against influenza in their blood after an influenza “season”. However, about three-quarters of infections were symptom-free, or so mild they weren't identified through weekly questioning about whether participants had a cough, cold, sore throat, or a “flu-like illness”.People who reported being ill were asked to take a nasal swab to test for the influenza virus. Among those with illnes...
Source: NHS News Feed - March 17, 2014 Category: Consumer Health News Tags: Swine flu Medical practice Source Type: news

Within-host influenza dynamics: A small-scale mathematical modeling approach.
Abstract The emergence of new influenza viruses like the pandemic H1N1 influenza A virus in 2009 (A(H1N1)pdm09) with unpredictable difficulties in vaccine coverage and established antiviral treatment protocols emphasizes the need of new murine models to prove the activity of novel antiviral compounds in vivo. The aim of the present study was to develop a small-scale mathematical model based on easily attainable experimental data to explain differences in influenza kinetics induced by different virus strains in mice. To develop a three-dimensional ordinary differential equation model of influenza dynamics, the follo...
Source: Biosystems - March 10, 2014 Category: Biotechnology Authors: Manchanda H, Seidel N, Krumbholz A, Sauerbrei A, Schmidtke M, Guthke R Tags: Biosystems Source Type: research

Spillover of pH1N1 to swine in Cameroon: an investigation of risk factors
Conclusions: Results suggest that the practice of allowing swine to roam freely is a significant risk factor for spillover of influenza from humans into swine populations. (Source: BioMed Central)
Source: BioMed Central - March 4, 2014 Category: Journals (General) Authors: Brenda LarisonKevin Y NjaboAnthony ChasarTrevon FullerRyan J HarriganThomas B Smith Source Type: research

Strategies for developing new antiviral flu drugs
New analysis of the influenza A virus by scientists at the University of Hertfordshire shows potential for developing new anti-viral drugs which are more likely to be universally effective against the flu virus originating from avian, swine or human virus strains. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - March 3, 2014 Category: Consumer Health News Tags: Flu / Cold / SARS Source Type: news

Prevalence of influenza a viruses in livestock and free-living waterfowl in Uganda
Conclusions: The study has demonstrated occurrence of influenza A viruses in animals in Uganda. The results suggest that increase in volumes of migratory waterfowl in the country could be associated with increased prevalence of these viruses in free-living waterfowl and poultry. (Source: BMC Veterinary Research - Latest articles)
Source: BMC Veterinary Research - Latest articles - February 27, 2014 Category: Veterinary Research Authors: Halid KirundaBernard ErimaAgnes TumushabeJocelyn KiconcoTitus TugumeSophia MuleiDerrick MimbeEdison MworoziJosephine BwogiLukwago LuswaHannah KibuukaMonica MillardAchilles ByaruhangaMariette DucatezScott KraussRichard WebbyRobert WebsterKofi WurapaDenis B Source Type: research

Prevalence of influenza A viruses in livestock and free-living waterfowl in Uganda
Conclusions: The study has demonstrated occurrence of influenza A viruses in animals in Uganda. The results suggest that increase in volumes of migratory waterfowl in the country could be associated with increased prevalence of these viruses in free-living waterfowl and poultry. (Source: BMC Veterinary Research - Latest articles)
Source: BMC Veterinary Research - Latest articles - February 27, 2014 Category: Veterinary Research Authors: Halid KirundaBernard ErimaAgnes TumushabeJocelyn KiconcoTitus TugumeSophia MuleiDerrick MimbeEdison MworoziJosephine BwogiLukwago LuswaHannah KibuukaMonica MillardAchilles ByaruhangaMariette DucatezScott KraussRichard WebbyRobert WebsterKofi WurapaDenis B Source Type: research

Bat-derived influenza-like viruses H17N10 and H18N11.
Abstract Shorebirds and waterfowls are believed to be the reservoir hosts for influenza viruses, whereas swine putatively act as mixing vessels. The recent identification of two influenza-like virus genomes (designated H17N10 and H18N11) from bats has challenged this notion. A crucial question concerns the role bats might play in influenza virus ecology. Structural and functional studies of the two major surface envelope proteins, hemagglutinin (HA) and neuraminidase (NA), demonstrate that neither has canonical HA or NA functions found in influenza viruses. However, putative functional modules and domains in other ...
Source: Trends in Microbiology - February 26, 2014 Category: Microbiology Authors: Wu Y, Wu Y, Tefsen B, Shi Y, Gao GF Tags: Trends Microbiol Source Type: research

Thanks to the FDA, Biocryst eyes finish line with peramivir
Durham drug developer BioCryst Pharmaceuticals announced encouraging news Tuesday morning when it said it successfully submitted a new drug application with federal regulators. The U.S. Food and Drug Administration accepted the BioCryst (Nasdaq: BCRX) application, called an NDA for short, for the drug peramivir, which would treat influenza strains including H7N9 and pandemic H1N1 swine flu viral strains. When the FDA agrees to accept an NDA, it essentially brings drug development of that new product… (Source: bizjournals.com Health Care:Physician Practices headlines)
Source: bizjournals.com Health Care:Physician Practices headlines - February 25, 2014 Category: American Health Authors: Jason deBruyn Source Type: research

A synchronized global sweep of the internal genes of modern avian influenza virus
Nature advance online publication 16 February 2014. doi:10.1038/nature13016 Authors: Michael Worobey, Guan-Zhu Han & Andrew Rambaut Zoonotic infectious diseases such as influenza continue to pose a grave threat to human health. However, the factors that mediate the emergence of RNA viruses such as influenza A virus (IAV) are still incompletely understood. Phylogenetic inference is crucial to reconstructing the origins and tracing the flow of IAV within and between hosts. Here we show that explicitly allowing IAV host lineages to have independent rates of molecular evolution is necessary for reliable phylogenetic...
Source: Nature AOP - February 16, 2014 Category: Research Authors: Michael WorobeyGuan-Zhu HanAndrew Rambaut Tags: Letter Source Type: research

A synchronized global sweep of the internal genes of modern avian influenza virus
Rambaut Zoonotic infectious diseases such as influenza continue to pose a grave threat to human health. However, the factors that mediate the emergence of RNA viruses such as influenza A virus (IAV) are still incompletely understood. Phylogenetic inference is crucial to reconstructing the origins and tracing the flow of IAV within and between hosts. Here we show that explicitly allowing IAV host lineages to have independent rates of molecular evolution is necessary for reliable phylogenetic inference of IAV and that methods that do not do so, including ‘relaxed’ molecular clock models, can be positi...
Source: Nature - February 16, 2014 Category: Research Authors: Michael WorobeyGuan-Zhu HanAndrew Rambaut Tags: Letter Source Type: research