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Effects of microinjection of angiotensin II and captopril to VTA on morphine self-administration in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study suggests the probable interaction between Ang II and opioid system in the VTA. PMID: 19700383 [PubMed - in process] (Source: Acta Biologica Hungarica)
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Hosseini M, Alaei HA, Havakhah S, Neemati Karimooy HA, Gholamnezhad Z Tags: Acta Biol Hung Source Type: journals

Ovarian histology and follicular score in female rats treated with nandrolone decanoate and submitted to physical effort.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The study was conducted to analyze the histology of the ovaries of adults rats treated with steroids, and submitted or not to physical effort. The control group consisted of females submitted to physical effort and sedentary females, both of which received a physiological solution of 0.9% saline. Treated females, sedentary or not, received 6 mg/kg of body weight of nandrolone decanoate. The steroid and physiological solution were administered intraperitoneally, with a single injection per week for 4 consecutive weeks. The applied physical effort was swimming (20 minutes daily, 5 days/week, for the 4 weeks of treatment)...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Cherici Camargo IC, Barreiros De Souza R, Fátima Paccola Mesquita S, Chuffa LG, Frei F Tags: Acta Biol Hung Source Type: journals

Biochemical markers of oxidative stress within tissues of cereal aphids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Effects of some dietary o -dihydroxyphenols on biochemical markers of oxidative stress within tissues of Sitobion avenae (Fabricius), (Homoptera, Aphididae) and Rhopalosiphum padi (Linnaeus), (Homoptera, Aphididae) has been studied. Among the studied aphid morphs the highest concentration of total thiols, hydrogen peroxide and lipid peroxidation products (TBARS) was noted for winged adults (alatae). Higher content of H 2 O 2 was observed within tissues of the oligophagous species R. padi while the monophagous species S. avenae had higher TBARS and total thiols concentration. Aphids exposed the dietary pro-oxidative o -...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Lukasik I, Goławska S, Leszczyński B Tags: Acta Biol Hung Source Type: journals

The effects of subchronic lithium administration in male Wistar mice on some biochemical parameters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lithium salts are efficiently used for treatment of psychiatric disorders. However, prolonged treatment frequently involves adverse side-effects. In the present work, effects of lithium carbonate administration on some biochemical parameters were studied in male mice. Lithium carbonate (20, 40 or 80 mg/kg body weight, corresponding to 3.77, 7.54 or 15.08 mg Li element/kg body weight, respectively) was injected daily for 14 or 28 days. The following parameters were recorded: water consumption, body weight, lithium and testosterone serum concentrations, activities of catalase (CAT), superoxide-dismutase (SOD) and glutath...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Nciri R, Allagui MS, Vincent C, Croute F, Elfeki A Tags: Acta Biol Hung Source Type: journals

Study on genes with altered expression in alpha-amanitin poisoned mice and evaluation on antagonistic effects of traditional Chinese medicines against toxicity of alpha-amanitin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The forward and reverse cDNA subtractive libraries before and after the toxic effect of alpha-amanitin were constructed by suppression subtractive hybridization and randomly selected clones from each subtractive library were screened by PCR and dot blot hybridization. A total of 85 genes with altered expression were finally identified, with 41 genes from the forward library and 44 from the reverse library. Subsequently, the antagonistic effects of candidate traditional Chinese medicines were evaluated based on the genetic transcription levels of the genes with significant altered expression, including Catnbeta, Flt3-L,...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Chen Q, Cao M, Xiang WL, Sun Q, Zhang J, Hou RT, Yan ZY, Yang ZR, Liu J, Zhao J Tags: Acta Biol Hung Source Type: journals

Whole effluent risk estimation for a small recipient watercourse.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Whole effluent toxicity is most often considered as a static parameter. However, toxicity might change as degradation processes, especially biodegradation goes by and intermediate products appear. These intermediates can even be more toxic than the original effluent was, posing higher risk to the ecosystem of the recipient water body. In our test series it was assessed how toxicity of a municipal wastewater sample changes during biodegradation taking into consideration different temperature regimes (10, 20 and 30 degrees C). Results proved our null hypothesis: after the high initial toxicity of the fresh effluent sampl...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Refaey M, Kováts N, Kárpáti A, Thury P Tags: Acta Biol Hung Source Type: journals

Somatic embryogenesis in apomict Boechera holboellii.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4-6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA - 6-benzylaminopurine; NAA - alpha-naphthaleneacetic acid; TDZ - 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 muM NAA + 8.87 muM BA and 2.68 muM NAA + 17.74 m...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Taskin KM, Turgut K, Scott RJ Tags: Acta Biol Hung Source Type: journals

Regeneration capacity of mature embryo-derived callus in barley ( Hordeum vulgare L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, induction of regenerable callus from mature embryos in eight Turkish barley varieties was analysed by using different plant growth regulators (PGRs). Varying concentrations (0.5-4 mg l -1 ) of 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro- o -anisic acid) were tested for callus induction from mature embryos. Highest percent of callus induction was observed in Bornova 92 variety (98.3%) on MS medium supplemented with 4 mg l -1 dicamba. Calli were transferred to regeneration media with 0.5 mg l -1 dicamba, 0.5 mg l -1 zeatin riboside (ZR) and 2 mg l -1 thidiazuron (TDZ). Low concentrations o...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Gürel F, Karakaş O, Albayrak G, Ari S Tags: Acta Biol Hung Source Type: journals

Optimization of coprogen production in Neurospora crassa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5 -monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which ...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Tóth V, Antal K, Gyémánt G, Miskei M, Pócsi I, Emri T Tags: Acta Biol Hung Source Type: journals

Characterization of the WILMS-TF microsatellite marker in Hungarian dog populations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Demand for correct and cost-effective genetic-based identification and parentage control has increasing importance in domestic animals, including dogs. In our study the applicability of a canine hyperpolymorphic microsatellite marker - which localized in the WILMS-TF (tumor factor) gene - was examined in mixed breed and purebred canine populations. The redesigned and shortened amplicons were genotyped using an allelic ladder which was constructed from sequence verified fragments. The nomenclature for allele calling based on repetition structures is suitable for international comparisons. Our study justified the potenti...
Source: Acta Biologica Hungarica - August 27, 2009 Category: Biology Authors: Zenke P, Maróti-Agóts A, Pádár Z, Zöldág L Tags: Acta Biol Hung Source Type: journals

Carnosine attenuates oxidative stress and apoptosis in transient cerebral ischemia in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cerebral ischemia leads to cognitive decline and neuronal damage in the hippocampus. Reactive oxygen species (ROS) play an important role in the neuronal loss after cerebral ischemia and reperfusion injury. Carnosine has both antioxidant and neuroprotective effects against ROS. In the present study, the effects of carnosine on oxidative stress, apoptotic neuronal cell death and spatial memory following transient cerebral ischemia in rats were investigated. Transient ischemia was induced by occlusion of right common carotid artery of rats for 30 min and reperfusion for 24 h or 1 week. Rats received intraperitoneal injec...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Pekcetin C, Kiray M, Ergur BU, Tugyan K, Bagriyanik HA, Erbil G, Baykara B, Camsari UM Tags: Acta Biol Hung Source Type: journals

Expression of two metallothionein genes in different brain regions of common carp.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd 2+ was investigated in the brain of common carp ( Cyprinus carpio ), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd 2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially;...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Ali KS, Ferencz A, Deér AK, Nemcsók J, Hermesz E Tags: Acta Biol Hung Source Type: journals

Ultrastructural aspects of previtellogenic oocyte growth in hermaphrodite sharpsnout seabream, Diplodus puntazzo (Teleostei, Sparidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This paper describes various aspects of previtellogenic oocyte growth in sharpsnout seabream, Diplodus puntazzo , is an important marine culture fish species in the Mediterranean. The ultrastructural characteristics of nuclear morphology, nuclear-cytoplasmic ratio and the starting of the follicle envelope formation were described in detail. These cells do not significantly differ from those of the other teleost species. The ultrastructural aspects provide new information on the reproductive biology of Sparidae. PMID: 19584025 [PubMed - in process] (Source: Acta Biologica Hungarica)
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Gülsoy N, Colak S Tags: Acta Biol Hung Source Type: journals

Effect of N-metylcarbamate pesticide bendiocarb on cattle lymphocytes after in vitro exposure.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bendiocarb is a carbamate broad-spectrum insecticide used to control disease vectors such as mosquitoes and flies, as well as household and agricultural pests. Nowadays, only few papers reporting cytogenetic or possible genotoxic effect of this insecticide on mammalian cells are available. In the present study 24-hour exposure to bendiocarbamate at concentrations ranging from 20 to 160 mug/ml was used for investigation of unstable chromosomal aberrations (CA), sister chromatid exchanges (SCE) and stable chromosomal aberration induction in cultured bovine peripheral lymphocytes. The slight but no significant increase of...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Holecková B, Siviková K, Dianovský J Tags: Acta Biol Hung Source Type: journals

Genotoxicity study of Hypiran and Chamomilla herbal drugs determined by in vivo supervital micronucleus assay with mouse peripheral reticulocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study Maximum Tolerated Dose (MTD) was obtained according to CSGMT protocol presented by the Environmental Mutagen Society of Japan. The positive group received mitomycin C in dose of 0.5 mg/kg. The peripheral blood samples before treatment (zero time) were considered as negative control. The MTD of Hypiron was 12 ml/kg and for Chamomilla was 10 ml/kg. Doses of MTD, 1/2 MTD and 1/4 MTD were considered for test groups. Then blood samples were prepared 48 hours after first administration of drugs and kept on precoated Acridine orange slides. The scoring of micronucleated reticulocytes were carried out per 2000 counte...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Kalantari H, Ashrafi Dashtearjandi A, Kalantar E Tags: Acta Biol Hung Source Type: journals

Effect of moderate hypoxia/reoxygenation on mitochondrial adaptation to acute severe hypoxia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In an experimental model, it was shown that repetitive periods of hypoxia/reoxygenation (H/R) [5 cycles of 5 min hypoxia (12% O 2 in N 2 ) followed by 15 min normoxia, daily for three weeks] attenuated basal and stimulated in vitro lipid peroxidation, as well as H 2 O 2 production in liver and brain mitochondria of rats exposed to acute severe hypoxia. Adaptation to moderate H/R enhanced in mitochondria the production and activity of reactive oxygen species scavengers, such as glutathione, manganese superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase. It was demonstrated that the maintenance of ...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Gonchar O, Mankovskaya I Tags: Acta Biol Hung Source Type: journals

UV-B response of greening barley seedlings.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The relationship between the greening stage of barley seedlings and their response to UV-B irradiation was studied. Etiolated barley seedlings ( Hordeum vulgare L., cv. Alfa) greened 12, 24 and 48 h were exposed to UV-B irradiation (312 nm) for 5 h. As a result of UV-B treatment the rate of CO 2 fixation and chlorophyll contents decreased but flavonoids, UV-B-induced compounds and carotenoids increased. The inhibition of photosynthesis in green plants was lower in comparison to greening ones. The 12 h greening plants were more sensitive to UV-B treatment than the plants greening 24 h and particularly 48 h, estimated by...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Fedina I, Velitchkova M, Georgieva K, Nedeva D, Cakırlar H Tags: Acta Biol Hung Source Type: journals

Effect of foliar feeding on nitrogen assimilation in alfalfa plants at insufficient molybdenum supply.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The influence of foliar feeding on the nitrogen assimilation in alfalfa plants under conditions of Mo shortage was studied. It was established that foliar fertilization with 0.3% solution of Agroleaf(R) resulted in increase of nitrogen fixation and nitrogen assimilation in the absence of Mo. Insufficient molybdenum supply leads to significant reduction of plant Mo content and nitrogen-fixing activity, while stress induced amino acids as alanine, GABA, threonine, proline and serine increased repeatedly. The negative effect of Mo deficiency on the enzyme activities related to the primary nitrogen assimilation (NR, GS, GO...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Hristozkova M, Geneva M, Stancheva I Tags: Acta Biol Hung Source Type: journals

Cytological changes in Turkish durum and bread wheat genotypes in response to salt stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Effects of salt stress on root growth, mitotic index, nuclear volume, vacuolization, nucleolar distortion and starch content were investigated in Turkish bread wheat ( Triticum aestivum L. cvs. Yildiz - salt sensitive, Dagdas - salt tolerant) and durum wheat ( Triticum durum L. cvs. C1252 - salt sensitive, Meramsalt tolerant) genotypes which were treated with 150 mM NaCI over a 6-day period. Salt treatment of wheat seedlings resulted in a decrease in root elongation and cell division in all genotypes at the 48 hours. According to controls, wheat root length decrease was 49% for Dagdas, 53.34% for Yildiz, 25.34% for Mer...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Yumurtaci A, Aydin Y, Uncuoglu AA Tags: Acta Biol Hung Source Type: journals

Shoot development from hypocotyl protoplasts of sunflower ( Helianthus annuus L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated mic...
Source: Acta Biologica Hungarica - May 31, 2009 Category: Biology Authors: Taski-Ajdukovic K, Nagl N, Miladinovic D, Mikic A Tags: Acta Biol Hung Source Type: journals

Type of cell death and the role of acetylcholinesterase activity in neurotoxicity induced by paraoxon in cultured rat hippocampal neurons.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, present data imply that the toxicity of paraoxon is both dose and duration dependent, which may even remain after the cessation of exposure. PMID: 19378919 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica)
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Bahrami F, Yousefpour M, Mehrani H, Golmanesh L, Sadraee SH, Khoshbaten A, Asgari A Tags: Acta Biol Hung Source Type: journals

Introducing tetraCys motifs at two different sites results in a functional dopamine transporter.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have introduced tetracysteine motifs into different positions of the dopamine transporter (DAT) for specific FlAsH labeling. Two of the constructs expressed at the cell surface and were functional as determined by [3H] dopamine uptake experiments. The N-terminally modified transporter showed uptake levels comparable to the wild-type DAT, while the construct with tetracysteine motif at position 511 displayed an uptake level about 1/3 of its wild-type counterpart. In addition, these two transporter constructs were visualized on the cell surface following labeling with a fluorescent cocaine analog. YFP introduced into ...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Orun O, Rasmussen S, Gether U Tags: Acta Biol Hung Source Type: journals

Ultrastructure of the mature spermatozoon of the bivalve Estellarca olivacea (Mollusca: Bivalvia: Arcidae) and its phylogenetic implications.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ultrastructure of mature spermatozoa of Estellarca olivacea was studied by transmission electron microscopy and its phylogenetic implications are discussed for the first time in this paper. The mature spermatozoon is composed of a head which contains a cone-shaped acrosome, a round nucleus and a tail region. The subacrosomal space is less electron dense which contains a homogeneous material. No axial rod and a basal plate were observed in subacrosomal space. No anterior invagination exists in the nucleus, but an inverted shallow V-shaped posterior invagination is visible. Nuclear lacunae could be seen clearly although ...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Zhu JQ, Yang WX Tags: Acta Biol Hung Source Type: journals

The effects of prometryne on subchronically treated mice evaluated by SCGE assay.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prometryne is a methylthio-s-triazine herbicide used to control annual broadleaf and grass weeds in many cultivated plants. Significant traces are documented in environment, mainly water, soil and plants used for human and domestic animal nutrition. Data on the toxic effects of prometryne and other methylthio-s-triazine have scorcely been published. The goal of this study was to investigate if prometryne, applied orally, could induce DNA damage in mouse leukocytes, in subchronical in vivo experimental design. Three different doses of prometryne were applied per os repeatedly every 48 hours. After the 7th dose (day 14) ...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Dikić D, Zidovec-Lepej S, Remenar A, Horvat-Knezević A, Benković V, Lisicić D, Sajli L, Springer O Tags: Acta Biol Hung Source Type: journals

The another toxic effect of carbamate insecticides.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after exposure to bendiocarb. In the liver, the activities of SOD, CAT and GR were not affected by bendiocarb. The induction or inhibition of isoenzymes of SOD (mainly MnSOD) were observed in the experimental groups. The activities of GSHPx-cum and GSHPx-H2O2 significantly decreased on the days 3 and 10 of the ...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Sobeková A, Holovská K, Lenártová V, Flesárová S, Javorský P Tags: Acta Biol Hung Source Type: journals

Preliminary studies on the effect of zinc and selenium on vanadium-induced cytotoxicity in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present work, we investigated the cytotoxicity of vanadium and the influence of zinc and selenium on vanadium-dependent cell damage in the BALB/c 3T3 cell culture. Treatment of cells for 24 hours with medium containing 50, 100 and 200 microM NaVO3 caused a significant decrease in the cell viability as measured by MTT test. Furthermore, the assays for reactive oxygen species (NBT reduction and phenol red oxidation) demonstrated the increase in superoxide and hydrogen peroxide production. In the cotreatment studies, the cells were exposed to NaVO3 (50, 100 and 200 microM) in the presence of nontoxic concentrations...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Zwolak I, Zaporowska H Tags: Acta Biol Hung Source Type: journals

Antioxidant and antiapoptotic activities of deprenyl and estradiol co-administration in aged rat kidney.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to determine whether the antioxidative effects of deprenyl can suppress apoptotic activity, with or without estradiol, in aged female rat kidney. Wistar Albino female rats were divided into six groups as follows; young (3 months old) control, aged (24 months old) control, aged deprenyl treated, aged estradiol treated, aged deprenyl plus estradiol treated and sham. All rats except for the sham group were injected for 21 days. Determination of oxidative stress parameter was performed spectrophotometrically. To detect apoptotic cells, TUNEL staining and caspase-3 immunohistochemistry were perfo...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Kiray M, Bagriyanik HA, Ergur BU, Pekcetin C, Topcu A Tags: Acta Biol Hung Source Type: journals

Changes in antioxidant status and biochemical parameters after orally cadmium administration in females rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The research was conducted to investigate the toxic effects of cadmium chloride (CdCl2), administered during gestation period on female Wistar rats. Pregnant rats received CdCl2 (20 mg/l, orally) from Day 6 to Day 19 of pregnancy. Results showed that Cd treatment induced a decrease in body weight gain. The relative liver weight increased significantly, with a marked decrease of glycogen and total lipids content. The administration of Cd induced hepatotoxicity as indicated by elevations in plasma alanine aminotransferase (ALT), aspartate aminotransferase and lactate dehydrogenase (LDH) activities (p < 0.05). Treatmen...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Chater S, Douki T, Favier A, Sakly M, Abdelmelek H Tags: Acta Biol Hung Source Type: journals

Comparison of murine fibroblast cell response to fluor-hydroxyapatite composite, fluorapatite and hydroxyapatite by eluate assay.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetic composite that contains the same molecular concentration of OH(-) groups and F(-) ions. The aim of this experiment was to evaluate the cellular responses of murine fibroblast NIH-3T3 cells in vitro to solid solutions of FHA and FA and to compare them with the effect of hydroxyapatite (HA). We studied 24, 48 and 72 h effects of biomaterials on cell morphology, proliferation and cell cycle of NIH-3T3 cells by eluate assay. Furthermore, we examined the ability...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Jantová S, Letasiová S, Theiszová M, Palou M Tags: Acta Biol Hung Source Type: journals

A comparative analysis of fatty acid composition of root and shoot lipids in Zea mays under copper and cadmium stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A comparative analysis of fatty acid composition was conducted in maize (Zea mays L.) under copper and cadmium stress. The unsaturation level (double-bond index) of phosphatidylethanolamine (PE) and digalactosyldiacylglycerol (DGDG) was increased in response to both metal treatments, whereas the phosphatidylinositol (PI), the phosphatidylcholine (PC) showed no significant changes. The Cu-treated roots showed a marked increase (about 2-fold) in the phospholipid (PL) content, while the Cd-treated roots showed a slight but insignificant increase. The steryl lipid SL/PL ratio was markedly decreased in response to Cu stress...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Chaffai R, Seybou TN, Marzouk B, El Ferjani E Tags: Acta Biol Hung Source Type: journals

Thermal inactivation kinetics of Lactococcus lactis subsp. lactis bacteriophage pll98-22.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Survival curves of Lactococcus lactis subsp. lactis bacteriophage pll98 inactivated by heat were obtained at seven temperature values (50-80 degrees C) in M17 broth and skim milk. Deviations from first-order kinetics in both media were observed as sigmoidal shapes in the survival curves of pll98. An empirical model with four parameters was used to define the thermal inactivation. Number of parameters of the model was reduced from four to two in order to increase the robustness of the model. The reduced model produced comparable fits to the full model. Both the survival data and the calculations done using the reduced m...
Source: Acta Biologica Hungarica - March 1, 2009 Category: Biology Authors: Sanlibaba P, Buzrul S, Akkoç N, Alpas H, Akçelik M Tags: Acta Biol Hung Source Type: journals

A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 p...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Shah AA, Hasan F, Hameed A, Ahmed S Tags: Acta Biol Hung Source Type: journals

Biological activity and identification of neuropeptides in the neurosecretory complexes of the cabbage pest insect, Mamestra brassicae (Noctuidae; Lepidoptera).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined brain-suboesophageal ganglia and corpora cardiaca-corpora allata complexes of the cabbage moth, Mamestra brassicae, in order to obtain clues about possible peptide candidates which may be appropriate for the biological control of this pest. With the aid of bioassays, reversed phase high performance liquid chromatography, and mass spectrometry, five neuropeptides were unequivocally identified and the presence of a further three were inferred solely by comparing mass spectra with known peptides. Only one neuropeptide with adipokinetic capability was identified in M. brassicae. Data from the established homologous...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Fónagy A, Marco HG, König S, Gäde G Tags: Acta Biol Hung Source Type: journals

Thyroidal inhibition following diverse stress in soft-shelled turtle, Lissemys punctata punctata bonnoterre.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The current study was undertaken to ascertain the effects of diverse stress on thyroid activity in soft-shelled turtles, Lissemys punctata punctata. The findings revealed that starvation (10 days), dehydration (10 days) or exposure to electric shock (12 volts for 15 seconds at an interval of 30 min for 3 h) caused significant decrease in the body weight (except in electric shock), relative weight, peripheral and central epithelial heights of the follicles and peroxidase activity of the thyroid gland of turtles. The degree of change in the values of these parameters was nearly same in all the stress experiments, indicat...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Ray PP, Sengupta A, Chaudhuri-Sengupta S, Maiti BR Tags: Acta Biol Hung Source Type: journals

Rat testicular mitochondrial antioxidant defence system and its modulation by aging.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Accumulation of oxidative damage caused by reactive oxygen species (ROS) underlies fundamental changes found during aging. In the present study, age related effect on testicular mitochondrial oxidant generation and antioxidant defence profile was investigated in Wistar rats at 3 months (young adults), 12 months (old adults) and 24 months (senescent animals) of age. Mitochondrial oxidative stress parameters viz., lipid peroxidation (LPx), protein carbonylation, hydrogen peroxide (H2O2) generation and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), levels of total, oxid...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Sahoo DK, Roy A, Chainy GB Tags: Acta Biol Hung Source Type: journals

Investigation of abiogenic stress-induced alterations in the level of secondary metabolites in poppy plants (Papaver somniferum L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We aimed to understand the effects of water stress on the alkaloid production in various developmental stages of poppy plants and the effect of stress on the alkaloids content in the capsules. Three stages of the life cycle of Papaver somniferum L. were selected in our studies: Rosette, Flowering and Lancing developmental stages. Four types of water conditions were examined: Control, Withdrawal of Water, 50% Water Supply and Inundation. The morphological monitoring, results of Relative Water Content and proline content were used as indicators of stress. The result of the measurements in poppy leaves show that the secon...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Szabó B, Lakatos A, Koszegi T, Botz L Tags: Acta Biol Hung Source Type: journals

Modeling and biochemical analysis of the activity of antibiofilm agent Dispersin B.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix (PGA), which is a linear polymer of beta(1,6)-linked N-acetylglucosamine (GlcNAc) residues. Dispersin B (DspB), a soluble glycoside hydrolase produced by the periodontal pathogen Actinobacillus actinomycetemcomitans degrades PGA. The enzyme DspB is an alpha/beta TIM-barrel protein and belongs to family 20 glycosyl hydrolases members. The enzyme activity of DspB with regard to its substrate specificity towards beta(1,6)-linked GlcNAc polymers and its endo/exo character was investigated through ligand docking and the hydrolysis o...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Kerrigan JE, Ragunath C, Kandra L, Gyémánt G, Lipták A, Jánossy L, Kaplan JB, Ramasubbu N Tags: Acta Biol Hung Source Type: journals

Effects of hyperbaric oxygenation on cultured barley embryos.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vulgare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, ...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Arican E, Gozukirmizi N Tags: Acta Biol Hung Source Type: journals

Trifluralin-induced disorganization of microtubular cytoskeleton alters the development of roots in Hordeum vulgare L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The extensive use of herbicides in agriculture becomes an important factor in environmental pollution, especially in case of slowly degradable compounds. Some agents act on plants during a long period of time, even if a very low concentration of the herbicide remains in the soil. Here, we investigated the toxicological effect of a low concentration of dinitroaniline herbicide, trifluralin, on growing seedlings of Hordeum vulgare L. Trifluralin in concentration of 1 microg/ml inhibited root growth. The mitotic activity of meristematic cells was suppressed due to the retardation of metaphase progression--alteration that ...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Sheval EV, Kazhura YI, Poleshuk NA, Lazareva EM, Smirnova EA, Maximova NP, Polyakov VY Tags: Acta Biol Hung Source Type: journals

The effect of silicon on the symptoms of manganese toxicity in maize plants.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effect of exogenously applied silicon (Si) on plant growth, lipid peroxidation, total phenolic compounds and non-protein thiols was studied in two maize varieties (Zea mays L. vars. Kneja 605, 434) differing in sensitivity to excess manganese (Mn). Based on the density of brown spots per leaf area and relative shoot weight (RSW) used to define Mn tolerance var. Kneja 434 was found to be more Mn-tolerant than Kneja 605. The lipid peroxidation level and total phenolic compounds were enhanced with increasing Mn concentration in the nutrient solution. In addition, the Mn-sensitive var. Kneja 605 with markedly expressed...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Stoyanova Z, Zozikova E, Poschenrieder C, Barcelo J, Doncheva S Tags: Acta Biol Hung Source Type: journals

A novel poly(3-hydroxybutyrate)-degrading Strepto verticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 p...
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Shah AA, Hasan F, Hameed A, Ahmed S Tags: Acta Biol Hung Source Type: journals

Changes in the leaf polypeptide patterns of wheat flag leaves spraying with non-purine cytokinins.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The changes in the polypeptide patterns of soluble proteins of wheat flag leaves after treatment with two synthetic cytokinin-like substances 3-CP-2-PU [1-(3-chlorophenyl)-3-(2-pyridyl)urea] and 3-CP-4-MPU [1-(3-chlorophenyl)-3-(4-methyl-2-pyridyl)urea] was studied. Both compounds increased differentially in quantity and composition most individual polypeptides identified compared to the non-treated leaves. While 3-CP-2-PU increased only the polypeptide quantity, 3-CP-4-MPU led to the appearance of new polypeptide with Mw of 51 kDa. PMID: 19133505 [PubMed - in process] (Source: Acta Biologica Hungarica)
Source: Acta Biologica Hungarica - December 1, 2008 Category: Biology Authors: Lazova G, Yonova P Tags: Acta Biol Hung Source Type: journals

Proceedings of the 11th Symposium of the International Society for Invertebrate Neurobiology. August 25-29, 2007. Tihany, Lake Balaton, Hungary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: PMID: 18773505 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica)
Source: Acta Biologica Hungarica - September 7, 2008 Category: Biology Tags: Acta Biol Hung Source Type: journals

Monitoring of neuroactive factors released from a pattern-generating network.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To detect neuroactive factors in biological fluids, we have previously elaborated a moveable biosensor, the preparation of isolated neuron penetrated with recording microelectrode. Here, we report that, in the pond snail, signals emanated from a central pattern generator (CPG) for feeding and detected by such biosensor correspond to long-term changes in motor output of the CPG as recorded from buccal nerves. The results suggest that combined recording from motor nerves, neurons in situ and the neuron-based biosensors may be a useful tool in studies of the mechanisms underlying network organisation. PMID: 18652368 [...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Chistopolsky IA, Vorontsov DD, Sakharov DA Tags: Acta Biol Hung Source Type: journals

Possible involvement of nitric oxide in coordination of buccal rhythm and gut motility in Lymnaea stagnalis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
NO-generating drugs (SNP, SNAP, NaNO2) activated the standard buccal rhythm and simultaneously slowed down the endogenous rhythm of the NO-synthesizing neuron B2 which enhances gut motility. In the B2, these drugs caused giant hyperpolarizing waves strongly associated with the buccal cycles. Hyperpolarizing waves on the B2 could also be induced by prolonged stimulation of the B2. The inhibitor of NO-synthase L-NNA or the NO scavanger PTIO abolished giant hyperpolarizing waves, depolarised the B2 and activated fast bursting in the B2. The results suggest that NO may be involved in coordination of radula and foregut move...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Dyakonova TL, Dyakonova VE Tags: Acta Biol Hung Source Type: journals

Functional neuroanatomy of the 5-HTergic system in the developing and adult buccal complex of the pond snail, Lymnaea stagnalis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Organization of the innervation of the buccal region by 5-HT-immunoreactive (IR) elements was investigated in the pond snail, Lymnaea stagnalis, with special attention to developmental aspects. A gradual maturation is characteristic for the 5-HT-IR muscle innervation, appearing first by late (E80-90%) embryogenesis. It runs parallel with the muscle development and the maturation of the 5-HTergic innervation in the buccal ganglia, peaking by the mid-postembryogenesis (P3) with the presence of a 5-HT-IR network in the buccal mass and rich innervation in the buccal ganglia, including axo-somatic contacts. The whole proces...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Balog G, Elekes K Tags: Acta Biol Hung Source Type: journals

Localization of serotonin transporter mRNA in the CNS of Lymnaea stagnalis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The serotonin transporter, SERT, is reported as a key molecule that regulates serotonergic neurotransmission. In the present study, we analyzed the localization of Lymnaea SERT (LymSERT) mRNA-containing neurons by in situ hybridization using frozen sections of the central nervous system (CNS) of Lymnaea. To precisely demonstrate the distribution of LymSERT mRNA-containing neurons, colocalization with serotonin immunoreactivity was also examined. The results showed that LymSERT mRNA was constitutively expressed and localized in the serotonin-containing neurons in the CNS. PMID: 18652373 [PubMed - indexed for MEDLINE...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Sadamoto H, Serfozo Z, Ito E Tags: Acta Biol Hung Source Type: journals

The effects of GABA on the network oscillations of the procerebrum in Limax valentianus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gamma-aminobutyric acid (GABA)-containing neurons are capable of controlling network oscillations and their patterns in the widely divergent species. In the terrestrial slug, Limax valentianus, oscillatory activity of the procerebrum neurons is considered to encode the odor information. Previous studies showed that GABA is present in the central nervous system and may be involved in the control of neuronal activity of Limax. In the present study, we examined the effects of GABA on the generation of oscillatory activity in the procerebrum. The results suggest that the GABAergic synaptic transmission may be involved in t...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Kobayashi S, Hattori M, Ito E Tags: Acta Biol Hung Source Type: journals

Neurophysiological analysis of visuo-vestibular conditioning in Lymnaea stagnalis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lymnaea are capable of learning an association between light and rotation. The conditioning paradigm to produce this association and the unconditioned escape response are the same for both Lymnaea and Hermissenda, but the underlying neural mechanisms appear to differ between the two species due to differences in the organization and function of their visuo- and vestibular-sensory systems. The RPeD11 interneuron in Lymnaea controls withdrawal behavior. We analyzed the electrophysiologic characteristics of the RPeD11 and observed an increase in cell excitability; that is, spontaneous activity of the postsynaptic potentia...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Suzuki H, Horikoshi T, Sakakibara M Tags: Acta Biol Hung Source Type: journals

Relationship between developmental synaptic modulation and conditioning-induced synaptic change in Lymnaea.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Though adult Lymnaea are bimodal breathers, young animals breathe mainly through the skin and adults through the lung. Operant conditioning changes adult breathing behavior from aerial to cutaneous. We hypothesized that this behavioral change is caused by alterations in the neuronal circuit during both development and conditioning. We focused our study on whether the synaptic connection between RPeD1 and RPA6 neurons is modulated during development and conditioning. Our findings indicated that the RPeD1 has an excitatory monosynaptic contact with the RPA6 in young naive and operantly-conditioned adult animals. The rela...
Source: Acta Biologica Hungarica - September 6, 2008 Category: Biology Authors: Karasawa T, Sato N, Horikoshi T, Sakakibara M Tags: Acta Biol Hung Source Type: journals