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Distribution of Notch family proteins in intrauterine growth restriction and hypertension complicated human term placentas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we aimed to clarify the immunolocalization of Notch proteins in full-term placentas after IUGR and PIH in comparison with normal placentas. Formalin-fixed, paraffin-embedded term placentas obtained by caesarean operations were processed for immunohistochemical localization of Notch 1, 2, 4 and Jagged 2. Transmission electron microscopy was also performed. In normal term placentas, all Notch proteins were intensely immunostained in the brush border of cells of the syncytiotrophoblast layer of the basal (maternal) side and the chorionic plate (fetal) side. The endothelial cells were also intensely immunostained...
Source: Acta Histochemica - November 11, 2009 Category: Biochemistry Authors: Sahin Z, Acar N, Ozbey O, Ustunel I, Demir R Tags: Acta Histochem Source Type: journals

The role of macrophages in the disappearance of Meckel's cartilage during mandibular development in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we observed morphological changes of Meckel's cartilage with development and analyzed the factors which might be related to this process. Meckel's cartilage of ICR strain mice from 14 to 19 days gestation (E14-19) were used in this study. Histological and immunohistochemical studies indicated the decrease in the amount of sulfated glycoconjugates and the localization of type I collagen in the Meckel's cartilage matrix during development. Chondrocytes also expressed high acid phosphatase activities at these stages. An organ culture study indicated that Meckel's cartilage at E17 disappeared during the cultivat...
Source: Acta Histochemica - October 22, 2009 Category: Biochemistry Authors: Tsuzurahara F, Soeta S, Kawawa T, Baba K, Nakamura M Tags: Acta Histochem Source Type: journals

Abelardo Gallego (1879-1930) and his contributions to histotechnology: The Gallego stains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Gallego general tissue stain is used in histology and histopathology and stains beautifully connective tissue, in which all nuclei are stained in magenta red, epithelial cytoplasm in red-yellow, connective tissue is stained in brilliant green, muscle in olive green, and keratinized epithelium and blood in grass green. There is also Gallego's method for differential staining of elastic tissue that enables a complete differentiation between elastic and collagenous connective tissue. The elastic tissue is stained in brilliant fuschin red, while collagen is stained in brilliant green. Abelardo Gallego was born in Spain...
Source: Acta Histochemica - October 22, 2009 Category: Biochemistry Authors: Ortiz-Hidalgo C Tags: Acta Histochem Source Type: journals

Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to show the effect of maternal exposure to flutamide on connexin 43 (Cx43) gene expression in testes and ovaries of 2-day-old piglets. Additionally, anogenital distance (AGD) was measured both in male and female offspring. Immunohistochemistry, Western blotting, and RT-PCR were performed to assess the immunoreactivity and the presence of Cx43 protein and it's mRNA, respectively. Following flutamide exposure strong immunostaining for Cx43 was observed between testicular Leydig cells, between granulosa cells of primary follicles, and between interstitial cells surrounding clusters of oocyte nest...
Source: Acta Histochemica - October 21, 2009 Category: Biochemistry Authors: Durlej M, Kopera I, Knapczyk-Stwora K, Hejmej A, Duda M, Koziorowski M, Slomczynska M, Bilinska B Tags: Acta Histochem Source Type: journals

Cellular localization of aquaporins along the secretory pathway of the lactating bovine mammary gland: An immunohistochemical study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we examined the cellular localization of aquaporins (AQPs) along the secretory pathway of actively lactating bovine mammary glands using immunohistochemistry. Mammary tissues examined included secretory ducts and acini, gland cisterns, teats, stromal and adipose tissues. Aquaporin 1 (AQP1) was localized in capillary endothelia throughout the mammary gland in addition to myoepithelial cells underlying teat duct epithelia. AQP2 and AQP6 were not detected and AQP9 was found only in leukocytes. AQP3 and AQP4 were observed in selected epithelial cells in the teat, cistern and secretory tubuloalveoli. AQP5 immunopo...
Source: Acta Histochemica - October 21, 2009 Category: Biochemistry Authors: Mobasheri A, Kendall BH, Maxwell JE, Sawran AV, German AJ, Marples D, Luck MR, Royal MD Tags: Acta Histochem Source Type: journals

PRDM16 expression in the developing mouse embryo.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PRDM16 is a member of the PR domain-containing protein family and is associated with various disease states including myelodysplastic syndrome and adult T-cell leukemia, as well as developmental abnormalities such as cleft palate. It is also known to act as a regulator of cell differentiation. Expression analysis of PRDM16 is limited, especially within the developing embryo. The current study evaluated the temporal and spatial localization of PRDM16 during early mouse development (embryonic days 8.5-14.5). PRDM16 was first detected on E9.5 in a limited number of tissues and by E14.5, was expressed in a broad range of d...
Source: Acta Histochemica - October 21, 2009 Category: Biochemistry Authors: Horn KH, Warner DR, Pisano M, Greene RM Tags: Acta Histochem Source Type: journals

Demonstration of substances of innate immunity in the esophageal epithelium of domesticated mammals. Part I - Methods and evaluation of comparative fixation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the investigation was to demonstrate that the esophageal epithelium of domesticated mammals exhibits characteristic features of innate immunity. The esophageal samples used were obtained immediately after euthanization from seven species of domesticated mammals of three nutrition groups (herbivores: horse, goat, cattle; omnivores: pig, dog, laboratory rat; carnivores: cat). The experimental basis was immunohistochemistry, which was evaluated in a qualitative and statistically relevant semi-quantitative manner. The first part of the study analyzed the influence of different fixation media on the immunohistoch...
Source: Acta Histochemica - October 21, 2009 Category: Biochemistry Authors: Nina Hornickel I, Kacza J, Schnapper A, Beyerbach M, Schoennagel B, Seeger J, Meyer W Tags: Acta Histochem Source Type: journals

Peptidyl arginine deiminase: A novel immunohistochemical marker for liver fibrosis in patients with chronic hepatitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objectives of this study therefore were to find out if PAD could be demonstrated immunohistochemically in liver biopsies of patients with chronic hepatitis and if it is associated with METAVIR activity and fibrosis scores. Liver biopsies were obtained from 100 patients with chronic liver diseases between September 2006 and 2007. The biopsies were scored by two histopathologists according to the METAVIR activity and fibrosis scores after histological preparation. Immunohistochemistry for PAD was performed on the biopsies using a monoclonal antibody against PAD. PAD could not be demonstrated in normal liver biopsies but ...
Source: Acta Histochemica - October 14, 2009 Category: Biochemistry Authors: Abdeen SM, Olusi SO Tags: Acta Histochem Source Type: journals

Prognostic relevance of cyclooxygenase-2 (COX-2) expression in Chinese patients with prostate cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cyclooxygenase-2 (COX-2), an inducible isoform of cyclooxygenase, has been reported to be correlated with tumorigenesis, tumor progression and metastasis. The present study was designed to investigate the clinicopathological and prognostic significance of COX-2 in Chinese patients with prostate cancer. Firstly, RT-PCR and Western blot assays were performed to detect the expression of COX-2 mRNA and protein in prostate cancer cell lines and 20 tissue samples (tumor or corresponding non-tumor). Next, immunohistochemistry was performed to detect the expression of COX-2 protein in 88 prostate cancer tissue samples. Finally...
Source: Acta Histochemica - October 13, 2009 Category: Biochemistry Authors: Bin W, He W, Feng Z, Xiangdong L, Yong C, Lele K, Hongbin Z, Honglin G Tags: Acta Histochem Source Type: journals

Immunohistochemical studies of the epididymal duct in Egyptian water buffalo (Bubalus bubalis).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion these findings showed a regional-specific distribution pattern, distinct from that in bovine bulls. Some potential functional capacities, especially absorptive and secretory ones, are discussed in relation to the different epididymal regions. PMID: 19836061 [PubMed - as supplied by publisher] (Source: Acta Histochemica)
Source: Acta Histochemica - October 13, 2009 Category: Biochemistry Authors: Alkafafy M, Elnasharty M, Sayed-Ahmed A, Abdrabou M Tags: Acta Histochem Source Type: journals

Abnormal cell-cycle expression of the proteins p27, mdm2 and cathepsin B in oral squamous-cell carcinoma infected with human papillomavirus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Since the role of human papillomavirus (HPV) infection in oral carcinogenesis is still unclear, the purpose of this study was to verify the association between the expression of p27, mdm2 and cathepsin B and by HPV-related oral lesions. Fifty-five oral biopsies were studied and HPV detection and typing (6/11, 16, 18, 31 and 33) were performed using polymerase chain reaction techniques. The distribution p27, mdm2 and cathepsin B was determined by immunohistochemistry. Twenty-one (38%) out of the 55 oral lesions tested positive for HPV, of which 6 (33%) were HPV 6/11, 1 (5%) was HPV 16, 14 (72%) were HPV 18 and none was ...
Source: Acta Histochemica - October 4, 2009 Category: Biochemistry Authors: Cristina Mazon R, Rovigatti Gerbelli T, Benatti Neto C, de Oliveira MR, Antonio Donadi E, Guimarães Gonçalves MA, Garcia Soares E, Patrícia Klay C, Tersariol I, Aparecida Pinhal M, Resende L, Pienna Soares C Tags: Acta Histochem Source Type: journals

Expression of apolipoprotein M in human hepatocellular carcinoma tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study examined mRNA levels and protein mass of apolipoprotein M (apoM) in human hepatocellular carcinoma (HCC) tissues and in the adjacent tissues. Plasma apoM levels in these HCC patients were also determined and compared to the normal subjects. The mean level of plasma apoM in the HCC patients was 0.61+/-0.30ODmm(-2), which was significantly higher than that in the normal subjects 0.37+/-0.07ODmm(-2) (P<0.01). However, both apoM mRNA levels and apoM protein mass in the HCC tissues were significantly lower than in the adjacent tissues (P<0.05). It is concluded that human hepatocellular carcinoma tiss...
Source: Acta Histochemica - September 28, 2009 Category: Biochemistry Authors: Jiang J, Wu C, Luo G, Zheng L, Chen L, Zhang X, Xu N Tags: Acta Histochem Source Type: journals

The effects of Gemcitabine and Vinorelbine on inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) distribution of MCF-7 breast cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, while G treatment, induced S-phase arrest, triggered the NOS pathway after treatment of MCF-7 cells, V treatment, arrested microtubule organization and did not change the NOS pathway. Detection of increased eNOS immunolabelling and apoptosis after G treatment of MCF-7 cells could be important to the treatment of human breast cancer. PMID: 19796794 [PubMed - as supplied by publisher] (Source: Acta Histochemica)
Source: Acta Histochemica - September 28, 2009 Category: Biochemistry Authors: Zeybek ND, Inan S, Ekerbicer N, Vatansever HS, Karakaya J, Muftuoglu SF Tags: Acta Histochem Source Type: journals

Expression of Slit2 and Robo1 after traumatic lesions of the rat spinal cord.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have used semi-quantitative RT-PCR, Western blot, and immunofluorescence imaging approaches to detect the expression levels of Slit2 and its receptor Robo1 in the rat spinal cord after traumatic lesions. Our results revealed that both the mRNA and protein levels of Slit2 were up-regulated in the injured spinal cord. The Slit2 expression level was increased at day 7 until day 14, and then returned to normal level at day 21 after injury. A double-immunolabelling study showed that Slit2 and neurofilament (NF) proteins were both localized in neurons of spinal corda cinerea. Slit2 immunopositivity was detected in neurona...
Source: Acta Histochemica - September 21, 2009 Category: Biochemistry Authors: Liu JB, Jiang YQ, Gong AH, Zhang ZJ, Jiang Q, Chu XP Tags: Acta Histochem Source Type: journals

Comparision of biocompatibility and cytotoxicity of two new root canal sealers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to investigate the remote organ toxicity and connective tissue reaction of two new root canal sealers ("GuttaFlow((R))" and "EndoREZ((R))") and to compare them with zinc oxide eugenol sealer using biochemical and histopathological parameters. A total of 60 white albino Wistar rats were used in the study. 0.1ml of GuttaFlow((R)), EndoREZ((R)) or Kerr Pulp Canal Sealer((R)) were administered subcutaneously into the mid-dorsal thoracic region of rats (15 in each group). Control rats were given saline only. Rats were decapitated after 24h, on day 7 and on day 30 of the experiment and tissue sample...
Source: Acta Histochemica - September 20, 2009 Category: Biochemistry Authors: Gencoglu N, Sener G, Omurtag GZ, Tozan A, Uslu B, Arbak S, Helvacioglu D Tags: Acta Histochem Source Type: journals

Multiple immunoenzyme labeling using heat treatment combined with the polymer method: An analysis of the appropriate inactivation conditions of primary antibodies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Multiple immunoenzyme labeling is of considerable value to detect several antigens in the same specimen, although this technique is limited when the primary antibodies have been raised in the same animal species. Multiple immunoenzyme labeling using heat treatment is a simple, reliable and straightforward technique wherein the heat treatment prevents mixed labeling and cross-reaction. The present study determined the inactivation time for primary antibodies by heat treatment in order to apply this procedure to routine histopathological diagnosis and research, and found that the inactivation time differed among the prim...
Source: Acta Histochemica - September 20, 2009 Category: Biochemistry Authors: Ikeda K, Suzuki T, Tate G, Mitsuya T Tags: Acta Histochem Source Type: journals

Methimazole-induced hypothyroidism causes cellular damage in the spleen, heart, liver, lung and kidney.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is known that a hypothyroidism-induced hypometabolic state protects against oxidative damage caused by toxins. However, some workers demonstrated that antithyroid drug-induced hypothyroidism can cause cellular damage. Our objective was to determine if methimazole (an antithyroid drug) or hypothyroidism causes cellular damage in the liver, kidney, lung, spleen and heart. Twenty-five male Wistar rats were divided into 5 groups: euthyroid, false thyroidectomy, thyroidectomy-induced hypothyroidism, methimazole-induced hypothyroidism (60mg/kg), and treatment with methimazole (60mg/kg) and a T(4) injection (20mug/kg/d sc)...
Source: Acta Histochemica - September 20, 2009 Category: Biochemistry Authors: Cano-Europa E, Blas-Valdivia V, Franco-Colin M, Gallardo-Casas CA, Ortiz-Butrón R Tags: Acta Histochem Source Type: journals

Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Immunolocalization of 3beta-hydroxysteroid dehydrogense (3beta-HSD), cytochrome P450c17 (P450c17) and androgen receptor (AR) were investigated in rat cumuli oophori (COCs) of late pre-ovulatory follicles and in post-ovulatory COCs bearing fertilized oocytes. A gradient of intensity of 3beta-HSD immunolabelling was observed in the granulosa layer of pre-ovulatory follicles, with almost negative immunolabelling in COCs and with the strongest immunoreaction in the mural granulosa cells. Post-ovulatory COCs showed strong 3beta-HSD immunolabelling in the peripheral regions and weak labelling near the oocyte, suggestive of r...
Source: Acta Histochemica - September 20, 2009 Category: Biochemistry Authors: Szołtys M, Słomczyńska M, Knapczyk-Stwora K, Durlej M, Lechowska A, Starowicz A Tags: Acta Histochem Source Type: journals

Immunolocalisation of laminin-1 in keratocystic odontogenic tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Keratocystic odontogenic tumors (KOTs) are distinct odontogenic lesions frequently affecting the jawbones. They may be associated with nevoid basal cell carcinoma syndrome (NBCCS), and may exhibit disorders involving the extracellular matrix. The aim of this study was to investigate the immunolocalisation of laminin-1 in 20 cases of KOTs in order to contribute to the characterization of this protein, which is little studied in odontogenic tumors. Our results showed laminin-1 in all 20 KOTs studied; its labelling intensity was weak in three cases (15%), moderate in five (25%) and strong in 12 cases (60%). Laminin-1 immu...
Source: Acta Histochemica - September 18, 2009 Category: Biochemistry Authors: Silva Gurgel CA, Gonçalves Ramos EA, Araújo Melo L, Brandi Schlaepfer C, de Souza RO, Campos Oliveira M, Dos Santos JN Tags: Acta Histochem Source Type: journals

Acidic polysaccharide of Panax ginseng as a defense against small intestinal damage by whole-body gamma irradiation of mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An acidic polysaccharide of Panax ginseng (APG), ginsan, has been reported to protect the hematopoietic system by increasing the number of bone marrow cells and spleen cells. Therefore, we evaluated the ability of APG to protect mice from radiation-induced damage of the small intestine. APG treatment caused the lengthening of villi and a numerical increase of crypt cells in the small intestine at 3.5 days after 7Gy irradiation compared to irradiated, non-treated controls. In addition, APG significantly inhibited irradiation-induced apoptosis by decreasing the amount of pro-apoptotic p53 and Bax as well as augmenting th...
Source: Acta Histochemica - September 17, 2009 Category: Biochemistry Authors: Park E, Hwang I, Song JY, Jee Y Tags: Acta Histochem Source Type: journals

Heterogeneous levels of oxidative phosphorylation enzymes in rat adrenal glands.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we morphologically and biochemically examined mitochondria in the rat adrenal gland. Immunohistochemistry showed that the rank order for intensity of immunolabelling for complex IV was zona reticularis > zona fasciculata adrenal medulla, whereas for complex V alpha and beta subunits, it was zona fasciculata > zona reticularis and adrenal medulla. The immunolabelling for complex I was homogeneous in the adrenal gland. The difference in immunolabelling between complexes I and IV indicates that the ratio of levels of complex I to that of complex IV in the zona reticularis was smaller than that in the zona...
Source: Acta Histochemica - September 17, 2009 Category: Biochemistry Authors: Ogawa K, Harada K, Endo Y, Sagawa S, Inoue M Tags: Acta Histochem Source Type: journals

Newly formed skeletal muscle fibers are prone to false positive immunostaining by rabbit antibodies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we demonstrate that newly formed myofibers are especially prone to false positive staining by rabbit antibodies and this unwanted staining is only recognized (1) by a negative muscle tissue control that does not harbor the protein to be examined (fx. from knockout mouse) or (2) by use of a nonsense rabbit antibody that has been prepared in the same way as the antibody of interest. However, many muscle immuno(cyto/histo)chemical studies only rely on controls that reveal non-specific binding by the secondary antibody and neglect that the primary rabbit antibody itself may cause false positive staining of the mu...
Source: Acta Histochemica - September 17, 2009 Category: Biochemistry Authors: Andersen DC, Kliem A, Schrøder HD, Jensen CH Tags: Acta Histochem Source Type: journals

Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH-diaphorase (NADPH-d) activity. Compared to control subjects, an increase in nitrergic activity was found in the molecular layer of dentate gyrus and in the lacunosum molecular and stratum radiatum of CA1 (cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal s...
Source: Acta Histochemica - September 10, 2009 Category: Biochemistry Authors: Maia CD, Ferreira VM, Kahwage RL, do Amaral MN, Serra RB, Noro Dos Santos S, do Nascimento JL, Rodrigues LG, Trévia N, Diniz CW Tags: Acta Histochem Source Type: journals

Albumin precursor and Hsp70 modulate corneal wound healing in an organ culture model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to investigate the role of albumin precursor and Hsp70 in corneal wound healing, we have analyzed the distribution of these proteins in wounded and non-wounded corneas of rabbits and the effects of topical applications of anti-albumin precursor and anti-Hsp70 antibodies on wound healing. Anti-albumin precursor and anti-Hsp70 antibodies were topically applied in healing corneal epithelium of rabbit eyes in organ culture. Corneas were allowed to heal in vitro for up to 120h in serum-free medium with 5 and 10mug/ml or without (migrating control) anti-albumin precursor/ or anti-Hsp70 antibodies. Fibronectin (Fb) (...
Source: Acta Histochemica - September 7, 2009 Category: Biochemistry Authors: Mushtaq S, Naqvi ZA, Siddiqui AA, Ahmed N Tags: Acta Histochem Source Type: journals

The immunohistochemical localization of the glycosphingolipid asialo-GM1 in the intestine of weaned piglets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The duodenum, jejunum, ileum, cecum and colon of three male hybrid piglets, 4 weeks old just after weaning, were investigated for the immunohistochemical localization of the asialoganglioside, GM1 (asialo-GM1). The study revealed various degrees of labelling for this acid glycosphingolipid in neural, epithelial and blood elements in all the gut segments. The immunolabelled neural structures, represented by ganglionic perikarya and nerve fibers, were distributed throughout the intestinal wall and showed quantitative variations in the various regions. In contrast the numerical evaluation of labelled epithelial cells was ...
Source: Acta Histochemica - September 6, 2009 Category: Biochemistry Authors: Trandaburu I, Oswald IP, Trandaburu T Tags: Acta Histochem Source Type: journals

Cilia-like structures anchor the amphioxus notochord to its sheath.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Body stiffness is important during undulatory locomotion in fish. In amphioxus, the myosepta play an important role in transmission of muscular forces to the notochord. In order to define the specific supporting role of the notochord in amphioxus during locomotion, the ultrastructure of 10 adult amphioxus specimens was analyzed using transmission electron microscopy. Numerous cilia-like structures were found on the surface of each notochordal cell at the sites of their attachment to the notochordal sheath. Ultrastructurally, these structures consisted of the characteristic arrangement of peripheral and central microtub...
Source: Acta Histochemica - September 6, 2009 Category: Biochemistry Authors: Bočina I, Ljubešić N, Saraga-Babić M Tags: Acta Histochem Source Type: journals

Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Propolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects. The target of this study was to investigate the anti-apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF-7 in culture. Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey. Individual extracts at 0.5, 0.25, 0.125 and 0.063mg/ml were incubated with MCF-7 cells during 2 days ...
Source: Acta Histochemica - September 2, 2009 Category: Biochemistry Authors: Seda Vatansever H, Sorkun K, Ismet Deliloğlu Gurhan S, Ozdal-Kurt F, Turkoz E, Gencay O, Salih B Tags: Acta Histochem Source Type: journals

Comparison of two techniques for the screening of human tumor cells in mouse blood: Quantitative real-time polymerase chain reaction (qRT-PCR) versus laser scanning cytometry (LSC).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The formation of metastases is often investigated in xenografted human tumors in mice and the need arises to detect disseminated human tumor cells in small volumes of mouse blood. Two techniques, namely quantitative real-time polymerase chain reaction (qRT-PCR) and laser scanning cytometry (LSC), were compared for screening of 100mul blood samples from immunodeficient mice spiked with a defined number of human HT29 colon carcinoma cells. With both techniques (qRT-PCR for amplifying of human Alu-sequences and LSC techniques for screening of fluorescence labelled cells), it was possible to detect single disseminated huma...
Source: Acta Histochemica - September 1, 2009 Category: Biochemistry Authors: Nehmann N, Wicklein D, Schumacher U, Müller R Tags: Acta Histochem Source Type: journals

Nitroxidergic system in human trigeminal ganglia neurons: A quantitative evaluation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The trigeminal ganglia are involved in transmission of orofacial sensitivity. The free radical gas nitric oxide (NO) has recently been found to function as a messenger molecule in both central and peripheral trigeminal primary afferent neurons. NO is produced within neurons mainly by two enzymes: a constitutive (neuronal) form of NO synthase (nNOS) or an inducible form of NOS (iNOS). The aim of the study was to evaluate the distribution of trigeminal neurons according to size (small, medium and large neurons) and to correlate the percentage of NOS-immunopositive neurons with regard to neuronal size. The results showed ...
Source: Acta Histochemica - September 1, 2009 Category: Biochemistry Authors: Borsani E, Giovannozzi S, Boninsegna R, Rezzani R, Labanca M, Tschabitscher M, Rodella LF Tags: Acta Histochem Source Type: journals

First János Fischer Memorial Symposium. Editorial.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
First János Fischer Memorial Symposium. Editorial. Acta Histochem. 2009;111(4):268 Authors: Makovitzky J, Richter S PMID: 19121534 [PubMed - indexed for MEDLINE] (Source: Acta Histochemica)
Source: Acta Histochemica - August 27, 2009 Category: Biochemistry Authors: Makovitzky J, Richter S Tags: Acta Histochem Source Type: journals

Proceedings of the first János Fischer Memorial Symposium, 15 September 2006, Szeged, Hungary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Proceedings of the first János Fischer Memorial Symposium, 15 September 2006, Szeged, Hungary. Acta Histochem. 2009;111(4):268-390 Authors: PMID: 19702003 [PubMed - indexed for MEDLINE] (Source: Acta Histochemica)
Source: Acta Histochemica - August 27, 2009 Category: Biochemistry Tags: Acta Histochem Source Type: journals

Nucleolar and cytoplasmic RNA density-concentration in leukemia granulocytic progenitors in human bone marrow biopsies: A short cytochemical note.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study was undertaken to provide more information on the differentiation and maturation of human granulocytes using computer-assisted image RNA densitometry at single-cell level. The bone marrow of patients suffering from chronic phase of chronic myeloid leukemia represents a very convenient model for such measurements because of the satisfactory number of early stages, as well as advanced stages, of the granulocytic cell lineage represented by neutrophils. In contrast to the erythroid cell lineage, similar nucleolar and cytoplasmic RNA density-concentration values were found only in early granulocytic proge...
Source: Acta Histochemica - August 18, 2009 Category: Biochemistry Authors: Smetana K, Jirásková I, Mikulenková D, Klamová H Tags: Acta Histochem Source Type: journals

Subcellular and regional location of "brain" proteins BASP1 and MARCKS in kidney and testis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Proteins BASP1 and MARCKS are abundant in axonal endings of neurons. Similarly to brain-specific protein GAP-43, BASP1 and MARCKS are reversibly bound to the plasma membrane. These proteins control both actin polymerization and actin cytoskeleton binding to the membrane. Performing these functions, BASP1 and MARCKS take part in growth cone guidance during development and in neurotransmitter secretion in adults. These activities predetermine the pivotal role of BASP1 and MARCKS in learning and memory. BASP1 and MARCKS were also found in non-nerve tissues, in particular, in the kidney and testis. Evidently, the physiolog...
Source: Acta Histochemica - August 13, 2009 Category: Biochemistry Authors: Mosevitsky M, Silicheva I Tags: Acta Histochem Source Type: journals

Phosphorylation of alpha-synuclein upregulates tyrosine hydroxylase activity in MN9D cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we provide evidence that tyrosine hydroxylase (TH) expression was unaffected by overexpression of wild-type and phospho-mimic mutant alpha-synuclein (S129D) in dopaminergic MN9D cells. However, alpha-synuclein overexpression evidently inhibited TH phosphorylation at Ser40 and dopamine synthesis, while alpha-synuclein (S129D) mutant enhanced TH phosphorylation and dopamine synthesis. This phospho-mimic mutant prevented wild-type alpha-synuclein cytotoxicity to MN9D cells, which might be due to aggregation of mutant alpha-synuclein in the cytoplasm and nuclei. These results demonstrated that phosphorylation at ...
Source: Acta Histochemica - August 12, 2009 Category: Biochemistry Authors: Wu B, Liu Q, Duan C, Li Y, Yu S, Chan P, Uéda K, Yang H Tags: Acta Histochem Source Type: journals

Differential regulation of FGF-2 in neurons and reactive astrocytes of axotomized rat hypoglossal nucleus. A possible therapeutic target for neuroprotection in peripheral nerve pathology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Despite the favorable treatment of cranial nerve neuropathology in adulthood, some cases are resistant to therapy leading to permanent functional impairments. In many cases, suitable treatment is problematic as the therapeutic target remains unknown. Basic fibroblast growth factor (bFGF, FGF-2) is involved in neuronal maintenance and wound repair following nervous system lesions. It is one of few neurotrophic molecules acting in autocrine, paracrine and intracrine fashions depending upon specific circumstances. Peripheral cranial somatic motor neurons, i.e. hypoglossal (XII) neurons, may offer a unique opportunity to s...
Source: Acta Histochemica - August 5, 2009 Category: Biochemistry Authors: de Oliveira GP, Duobles T, Castelucci P, Chadi G Tags: Acta Histochem Source Type: journals

Nervous regulation of internal organs in fishes. Preface.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19091387 [PubMed - indexed for MEDLINE] (Source: Acta Histochemica)
Source: Acta Histochemica - August 5, 2009 Category: Biochemistry Authors: Olsson C, Jonz M, Zaccone G Tags: Acta Histochem Source Type: journals

Nervous control of circulation--the role of gasotransmitters, NO, CO, and H2S.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The origins and actions of gaseous signaling molecules, nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) in the mammalian cardiovascular system have received considerable attention and it is evident that these three "gasotransmitters" perform a variety of homeostatic functions. The origins, actions and disposition of these gasotransmitters in the piscine vasculature are far from resolved. In most fish examined to date, NO or NO donors are generally in vitro and in vivo vasodilators acting via soluble guanylyl cyclase, although there is evidence for NO-mediated vasoconstriction. Injection of sodium n...
Source: Acta Histochemica - August 5, 2009 Category: Biochemistry Authors: Olson KR, Donald JA Tags: Acta Histochem Source Type: journals

Sensory innervation of the Gills: O2-sensitive chemoreceptors and mechanoreceptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Physical characteristics of water (O(2) solubility and capacitance) dictate that cardiovascular and ventilatory performance be controlled primarily by the need for oxygen uptake rather than carbon dioxide excretion, making O(2) receptors more important in fish than in terrestrial vertebrates. An understanding of the anatomy and physiology of mechanoreception and O(2) chemoreception in fishes is important, because water breathing is the primitive template upon which the forces of evolution have modified into the various cardioventilatory modalities we see in extant terrestrial species. Key to these changes are the O(2)-...
Source: Acta Histochemica - August 5, 2009 Category: Biochemistry Authors: Burleson ML Tags: Acta Histochem Source Type: journals

Molecular-assisted immunohistochemical optimization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report our experience conducting an IHC optimization of antibodies against five proteins previously identified by proteomic analysis to be breast cancer biomarkers, namely 6PGL (PGLS), CAZ2 (CAPZA2), PA2G4 (EBP1) PSD2 and TKT. Large variations in the immunolocalizations and intensities were observed when manipulating the antigen retrieval method and primary antibody incubation concentration. However, the use of an independent molecular analysis method provided a clear indication in choosing the appropriate biologically and functionally relevant "staining pattern". Without this latter step, each of these c...
Source: Acta Histochemica - July 20, 2009 Category: Biochemistry Authors: Mohd Omar MF, Huang N, Ou K, Yu K, Putti TC, Jikuya H, Ichikawa T, Nishimura O, Tan P, Salto-Tellez M Tags: Acta Histochem Source Type: journals

High cholesterol diet increases MMP9 and CD40 immunopositivity in early atherosclerotic plaque in rabbits.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Inflammation is one of the important contributing factors for the development of atherosclerosis and heart disease. Inflammation leads to the mobilization of various cells in developing atherosclerotic plaque and simultaneously triggers the up-regulation of various cytokine secretions from effector cells. To understand early molecular events during atherosclerosis we developed a rabbit model in which male New Zealand White rabbits were fed high cholesterol diets for 12 weeks. Total cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C) and high sensitivity C-reactive protein (hs-CRP) were sig...
Source: Acta Histochemica - July 14, 2009 Category: Biochemistry Authors: Mathapati S, Arumugam SB, Verma RS Tags: Acta Histochem Source Type: journals

Histochemical analysis of muscle fiber types of rat superior rectus extraocular muscle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Extraocular muscles (EOMs) represent a distinctive class among mammalian skeletal muscles in exhibiting unique anatomical and physiological properties. To gain insight into the basis for the unique structural/functional diversity of EOM fiber types and to explain their high fatigue resistance, rat superior rectus muscle (SRM) was studied using histochemical techniques. Muscle fibers were typed with regard to their oxidative and glycolytic profiles generated from succinic dehydrogenase (SDH) and phosphorylase activities in combination with their morphologic characteristics. Superior rectus muscle is organized into two l...
Source: Acta Histochemica - July 13, 2009 Category: Biochemistry Authors: Rashed RM, El-Alfy SH, Mohamed IK Tags: Acta Histochem Source Type: journals

Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos - E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage...
Source: Acta Histochemica - July 13, 2009 Category: Biochemistry Authors: Paiva KB, Silva-Valenzuela MD, Massironi SM, Ko GM, Siqueira FM, Nunes FD Tags: Acta Histochem Source Type: journals

High levels of connexin 43 mRNA in high grade astrocytomas. Study of 32 cases with in situ hybridization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study showed that the immunohistochemical labelling of Cx43 is reduced in grade III and grade IV. Decreased gap junction-mediated intercellular communication corresponds to decreased Cx43 and increased cellular proliferation rates, as measured by immunolabelling of the Ki67 nuclear antigen. This study demonstrated also the persistence in high grade astrocytomas of elevated levels of Cx43 mRNA. The reduced levels of Cx43 protein should not to be ascribed to a reduced genetic transcription, but to an alteration of the post-transcriptional mechanisms such as the regulation of its synthesis and/or the intracellular transp...
Source: Acta Histochemica - July 12, 2009 Category: Biochemistry Authors: Caltabiano R, Torrisi A, Condorelli D, Albanese V, Lanzafame S Tags: Acta Histochem Source Type: journals

Liver immunolocalization and plasma levels of MMP-9 in non-alcoholic steatohepatitis (NASH) and hepatitis C infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Non-alcoholic steatohepatitis (NASH) is a progressive fibrotic disease. Many issues related to the pathogenesis of this disease remain unresolved. Because of NASH association with the activation of liver fibrogenesis, we examined the plasma levels and liver immunolocalization of matrix metalloprotease-9 (MMP-9), a molecule involved in the remodelling processes of fibrogenesis. In addition, patients with chronic hepatitis C (HCV) were analyzed. Plasma concentrations of MMP-9 were determined by ELISA from peripheral blood and immunohistochemistry of the same protein was carried out in formalin-fixed and paraffin wax-embe...
Source: Acta Histochemica - July 12, 2009 Category: Biochemistry Authors: D'Amico F, Consolo M, Amoroso A, Skarmoutsou E, Mauceri B, Stivala F, Malaponte G, Bertino G, Neri S, Mazzarino MC Tags: Acta Histochem Source Type: journals

The existence of gonadotropin-releasing hormone-like peptides in the neural ganglia and ovary of the abalone, Haliotis asinina L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have demonstrated the presence and distribution of two isoforms of GnRH-like peptides in neural ganglia and ovary of reproductively mature female abalone, Haliotis asinina, using immunohistochemistry. We found significant immunoreactivities (ir) of anti-lamprey(l) GnRH-III and anti-tunicate(t) GnRH, but with variation of labeling intensity by each anti-GnRH type. lGnRH-III-ir was detected in numerous type 1 neurosecretory cells (NS1) throughout the cerebral and pleuropedal ganglia, whereas tGnRH-I-ir was detected in only a few NS1 cells in the dorsal region of cerebral and pleuropedal ganglia. In addition...
Source: Acta Histochemica - July 12, 2009 Category: Biochemistry Authors: Nuurai P, Poljaroen J, Tinikul Y, Cummins S, Sretarugsa P, Hanna P, Wanichanon C, Sobhon P Tags: Acta Histochem Source Type: journals

Immunolocalization of FGF1 and FGF2 in the regenerating tail of the lizard Lampropholis guichenoti: Implications for FGFs as trophic factors in lizard tail regeneration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A role for fibroblast growth factors in stimulating limb and tail regeneration in amphibians has been shown; however, it is unknown whether these growth factors are also involved in the regeneration of the tail of lizard, an amniote model for studies on tissue regeneration. The presence of fibroblast growth factor-1 (FGF1) and -2 (FGF2) in the regenerating tail of the lizard Lampropholis guichenoti has been studied using immunofluorescence labeling. The study reveals that FGF2 is mainly localized in the wound and scaling epidermis, in differentiating muscles, in spinal ganglia, regenerating nerves and spinal cord. FGF1...
Source: Acta Histochemica - July 6, 2009 Category: Biochemistry Authors: Alibardi L, Lovicu FJ Tags: Acta Histochem Source Type: journals

Modifications of the lactate dehydrogenase assay, a histochemical determinant of osteocyte viability--a qualitative study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the effect of (a) concentration of coenzyme and tetrazole (b) bone specimen thickness (c) ability to use frozen sections and (d) incubation time/dilution. We concluded that a 1000-fold increase in the concentration of coenzyme and tetrazole were required. Fresh bone produced optimal results and near-complete viability. Special considerations must be taken with smaller, more fragile specimens (e.g., mouse bone), such as increasing specimen thickness, dilution of incubation medium and/or the reduction of incubation time. Sections from thawed frozen bone resulted in a diffuse reaction. Osteocyte viability can be a...
Source: Acta Histochemica - June 28, 2009 Category: Biochemistry Authors: Phillips CA, Hughes DR, Huja SS Tags: Acta Histochem Source Type: journals

Effects of Nigella sativa seed extract on ameliorating lung tissue damage in rats after experimental pulmonary aspirations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aspiration of gastric contents can cause serious lung injury, although the mechanisms of pulmonary damage are still not clear and means of amelioration of the pulmonary damage have been little investigated. The black cumin seed, Nigella sativa L. (NS) has been shown to have specific health benefits and the aim of the current study was to investigate the possible beneficial effects of NS on experimental lung injury in male Wistar rats after pulmonary aspiration of different materials. The rats were randomly allotted into one of six experimental groups (n=7 per group): (1) saline control, (2) saline+NS treated, (3) Pulmo...
Source: Acta Histochemica - June 28, 2009 Category: Biochemistry Authors: Kanter M Tags: Acta Histochem Source Type: journals

To the memory of Prof. Dr. Rer. Nat. János Fischer (1945-1999), a pioneer of sugar histochemistry and biochemistry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To the memory of Prof. Dr. Rer. Nat. János Fischer (1945-1999), a pioneer of sugar histochemistry and biochemistry. Acta Histochem. 2009;111(4):269-72 Authors: Emödy L, Makovitzky J PMID: 19514118 [PubMed - in process] (Source: Acta Histochemica)
Source: Acta Histochemica - June 28, 2009 Category: Biochemistry Authors: Emödy L, Makovitzky J Tags: Acta Histochem Source Type: journals

Ovarian hormones control the changing expression of claudins and occludin in rat uterine epithelial cells during early pregnancy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulation of the uterine luminal environment is important for successful attachment and implantation of the blastocyst. The contents and volume of luminal fluid are regulated in part by the tight junctions. Using immunofluorescence microscopy, protein and RNA analysis, the cellular distributions of tight junction components claudins and occludin were observed during early pregnancy and under various hormonal regimens. Results indicate that occludin and claudin-4 distribution changed during early pregnancy and in response to ovarian hormones. At the time of implantation and in response to progesterone administration to...
Source: Acta Histochemica - June 22, 2009 Category: Biochemistry Authors: Nicholson MD, Lindsay LA, Murphy CR Tags: Acta Histochem Source Type: journals