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Abstracts of the Annual Meeting of the Hungarian Society for Microbiology, October 15-17, 2008 Keszthely, Hungary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: PMID: 19828428 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 30, 2009 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: journals

Immunosenescence and vaccination of the elderly I. Age-related immune impairment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The sharp increase of life expectancy and the increasing ratio of ageing population pose new challenges for the public health system. The elderly suffer from more frequent and severe infections than young people. Theoretically, vaccination could protect the elderly against several infectious diseases, but due to their age-related immune impairment, vaccination might fail in many cases. Instead of ineffective vaccination campaigns, exploration and restoration of age-dependent dysregulation of their immune functions have to be placed into the focus of recent research. Frequent comorbidities in these people augment immune...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Ongrádi J, Stercz B, Kövesdi V, Vértes L Tags: Acta Microbiol Immunol Hung Source Type: journals

RpoS-independent and growth phase-dependent expression of dcuSR operon of Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The dcuSR operon of Escherichia coli encodes a two-component sensor/kinase-response/regulator system. This system regulates gene expression in response to external C 4 -dicarboxylates. During entry into stationary phase Gram-negative bacteria express genes that impart cellular resistance to environmental stresses. In E. coli , 50 or more genes are triggered by sigma factor ( sigma s ) during entry into stationary phase. Multi-copy dcuS-lacZ and chromosomally integrated dcuS-lacZ fusions analysis showed that the expression of dcuSR is positively regulated during growth phase. Many genes that are required for stationary-...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Abo-Amer AE, Altalhi AD Tags: Acta Microbiol Immunol Hung Source Type: journals

Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. In the present study, immobilization of a purified alkaline extra-cellular lipase of Bacillus cereus MTCC 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. B. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. Immobilized lipase was used to synthesis of ethyl acetate from vinyl acetate and ethanol in n -nonane. Various reaction condit...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Verma ML, Azmi W, Kanwar SS Tags: Acta Microbiol Immunol Hung Source Type: journals

Fungal endophytes in three medicinal plants of Lamiaceae.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three medicinal plants Ocimum sanctum, Ocimum bacilicum and Leucas aspera were screened to study endophytic diversity of the plants. Altogether 103 fungal endophytes belonging to fourteen genera were isolated. Leaves of all three medicinal plants were colonized by a great number of endophytic fungi. Leaves of O. sanctum were colonized by the most, that is, eleven endophytes. Highest Shannon-Wiener index (2.256) was exhibited by O. sanctum with the highest Simpson's diversity (0.8654) indicating great species specificity. O. bacilicum and L. aspera showed the highest similarity coefficient. Some fungal genera have been ...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Banerjee D, Manna S, Mahapatra S, Pati BR Tags: Acta Microbiol Immunol Hung Source Type: journals

Risk factors and outcomes for bloodstream infections with extended-spectrum beta -lactamase-producing Klebsiella pneumoniae ; Findings of the nosocomial surveillance system in Hungary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Risk factors for and outcomes of bloodstream infections (BSIs) caused by ESBL-producing and by ESBL-non-producing Klebsiella pneumoniae were compared in a four-year multicenter study in Hungary. One hundred ESBL-positive and one hundred ESBL-negative patients were included as cases and controls. Investigated risk factors were related to demographics, comorbid conditions, treatments, invasive procedures, surgery prior bacteremia, presence of additional nosocomial infections and preceding hospital admission within a year. Measured outcomes were crude mortality, mortality related to infection and delay in introducing appr...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Szilágyi E, Füzi M, Böröcz K, Kurcz A, Tóth A, Nagy K Tags: Acta Microbiol Immunol Hung Source Type: journals

Plant growth promotion by phosphate solubilizing bacteria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Most agronomic soils contain large reserves of total phosphorus [P], but the fixation and precipitation of P cause P deficiency, and in turn, restrict the growth of crops severely. Phosphorus replenishment, especially in sustainable production systems, remains a major challenge as it is mainly fertilizer-dependent. Though the use of chemical P fertilizers is obviously the best means to circumvent P deficiency in different agro-ecosystems, their use is always limited due to its spiralling cost. A greater interest has, therefore, been generated to find an alternative yet inexpensive technology that could provide sufficie...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Zaidi A, Khan MS, Ahemad M, Oves M Tags: Acta Microbiol Immunol Hung Source Type: journals

Microbial communities in bees, pollen and honey from Slovakia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
As the honey-bee gastrointestinal tract microflora and pollen are the primary sources for the honey microbial community, the aim of this work was to study and characterize the microbial transit among them. Therefore, an exhaustive microbial analysis of honey, adult honey-bee gastrointestinal tract, and pollen from different Slovakian regions and different seasons, was conducted. Microbial screening revealed that the primary sources of microbial community present in Slovakian honey are pollen and the honey-bees' digestive tract microflora, containing microorganisms normally present in dust, air and flowers. We found tha...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Kacániová M, Pavlicová S, Hascík P, Kociubinski G, Kńazovická V, Sudzina M, Sudzinová J, Fikselová M Tags: Acta Microbiol Immunol Hung Source Type: journals

In memoriam D. C. Gajdusek (1923-2008).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19789143 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2009 Category: Microbiology Authors: Rajcáni J Tags: Acta Microbiol Immunol Hung Source Type: journals

Sixtieth anniversary of the Institute of Medical Microbiology, Semmelweis University, Budapest (1948-2008)--reminiscences of sixty years in the institute.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19621764 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Nász I Tags: Acta Microbiol Immunol Hung Source Type: journals

Production of poly-3-hydroxyalkanoic acids by a moderately halophilic bacterium, Halomonas marina HMA 103 isolated from solar saltern of Orissa, India.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study is an attempt to optimize the cultural conditions for efficient production of P(3HB) by H. marina in batch cultivation. Growth of the organism under shake-flask culture using 2% (w/v) glucose resulted in P(3HB) accumulation accounting for more than 59% of cell dry weight after 50 h of incubation. The optimum P(3HB) production was attained with a combined supply of NH4Cl and yeast extract as N-source, 0.01% (w/v) phosphate, 1.5% (w/v) sulphate and 10% (w/v) NaCl. Qualitative and quantitative 1HNMR and FT-IR analysis of cells grown in alkanoic acids (C3-C6) as sole source of carbon and co-substrates revealed synth...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Biswas A, Patra A, Paul AK Tags: Acta Microbiol Immunol Hung Source Type: journals

Comparison of two clinic-based immunoassays with the immunofluorescence antibody test for the field diagnosis of canine monocytic ehrlichiosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to compare a clinic-based enzyme-linked immunosorbent assay (immunocomb-Eliza, Biogal, Israel) and a field-based dot-blot enzyme-linked immunoassay (DBELIA) with the indirect immunofluoresence antibody test (IFAT) used for the field diagnostics of canine monocytic ehrlichiosis (CME). The total test samples was 60; out of them 52 serum samples came from naturally exposed police German shepherd dogs and 8 from adult mongrels infected with viable Ehrlichia canis propagated in a dog macrophage laboratory cell line (DH82). The DBELIA test was positive in 86.7% (56/60) and negative in 13.3% (8/60), ...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Okewole EA, Adejinmi JO Tags: Acta Microbiol Immunol Hung Source Type: journals

Proteomics view of a Rhizobium isolate response to arsenite [As(III)] stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The genus Rhizobium colonizes in leguminous plants by symbiotic relationship and enriches soil nitrogen through the formation of the root nodule. Legumes are the appropriate crops for the recovery of marginal lands and they can easily grow in adverse climatic condition. Rhizobium sp. VMA301 was isolated from the root nodules of V. mungo, grown in arsenic contaminated field. The LC50 value of arsenite for VMA301 was found to be 1.8 mM. Sixteen differentially expressed proteins were identified using RP-HPLC and MALDI ToF mass spectrometry from arsenite induced whole cell lysate soluble proteins. It is also found that nin...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Mandal SM, Mandal M, Pati BR, Das AK, Ghosh AK Tags: Acta Microbiol Immunol Hung Source Type: journals

Screening and selection of lovastatin hyper-producing mutants of Aspergillus terreus using cyclic mutagenesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
134 fungal cultures isolated from different soil samples were screened for lovastatin production. Of these, 38 isolates produced different levels of lovastatin. An Aspergillus terreus strain GD13, producing 190 mg/l of lovastatin was selected and subjected to a rational mutation-selection programme based on the resistance to lovastatin and fatty acid synthase (FAS) inhibitors, viz., iodoacetamide and N-ethylmaleimide. After three cycles of mutagenesis, a hyper-producing mutant (EM19) exhibiting 7.5-fold (1424 mg/l) higher levels of lovastatin when compared to wild type parent strain was obtained. PMID: 19621768 [Pu...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Kaur H, Kaur A, Saini HS, Chadha BS Tags: Acta Microbiol Immunol Hung Source Type: journals

Molecular detection of T. pallidum by PCR in seronegative cases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For the molecular detection of Treponema pallidum authors introduced and used a nested PCR amplifying a conservative portion of the gene coding for the Tp 47 kDa membrane protein. PCR verified the presence of T. pallidum specific DNA in 5.7 per cent of syphilis seronegative 105 MSM belonging to HIV risk group. Treponema DNA was also detected in HIV infected, syphilis seronegative cases. Specificity of the method was demonstrated in rabbit inoculation test and also in clinically positive syphilis cases. PMID: 19621769 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Talha E, Juhász E, Kanizsai S, Nagy K Tags: Acta Microbiol Immunol Hung Source Type: journals

Immunohistochemical analysis of granzyme B in non-perforated appendicitis--short communication.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the present study was to determine the expression ofgranzyme B in non-perforated appendicitis. Appendix biopsies were obtained from the patients with clinically diagnosed as acute appendicitis and subjects admitted for elective abdominal surgery. All biopsies from the patients were non-perforated and histologically divided into acute and non-acute appendicitis. Granzyme B expression was assessed immunohistochemically. The results showed that granzyme B expression in both acute and non-acute appendicitis was significantly lower than that in the control appendix tissues (P < 0.05). The expression of this cy...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2009 Category: Microbiology Authors: Suyarsono , Budyani DR, Sosroseno W Tags: Acta Microbiol Immunol Hung Source Type: journals

Microbe-induced epigenetic alterations in host cells: the coming era of patho-epigenetics of microbial infections. A review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is well documented that the double-stranded DNA (dsDNA) genomes of certain viruses and the proviral genomes of retroviruses are regularly targeted by epigenetic regulatory mechanisms (DNA methylation, histone modifications, binding of regulatory proteins) in infected cells. In parallel, proteins encoded by viral genomes may affect the activity of a set of cellular promoters by interacting with the very same epigenetic regulatory machinery. This may result in epigenetic dysregulation and subsequent cellular dysfunctions that may manifest in or contribute to the development of pathological changes (e.g. initiation and...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Minárovits J Tags: Acta Microbiol Immunol Hung Source Type: journals

Clinical microbiology of early-onset and late-onset neonatal sepsis, particularly among preterm babies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prematurity has got special challenge for clinicians and also other medical staff, such as microbiologists. Immature host defense mechanisms support early-onset sepsis, which can be very serious with very high mortality. While the past decade has been marked by a significant decline in early-onset group B streptococcal (GBS) sepsis in both term and preterm neonates, the overall incidence of early-onset sepsis has not decreased in many centers, and several studies have found an increase in sepsis due to gram-negative organisms. With increasing survival of these more fastidious preterm infants, late-onset sepsis or speci...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Kristóf K, Kocsis E, Nagy K Tags: Acta Microbiol Immunol Hung Source Type: journals

Broadening spectrum of bovine spongiform encephalopathies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Until recently the etiology of bovine spongiform encephalopathy (BSE) was considered uniform. The infectious agent was thought to be a single strain of prion (posttranslationally altered form of normal prion protein: PrPSc) retaining its biochemical and biological characteristics during interspecies transmission. However, alternate PrPSc signatures through large-scale screening have recently been detected. In addition, genetic alterations governing susceptibility to prion infection and a mutation (E211K) capable of eliciting spontaneous BSE have been demonstrated. Thus, the spectrum of BSEs have broadened and three PrP...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Füzi M, Horváth P Tags: Acta Microbiol Immunol Hung Source Type: journals

Growth, keratinolytic proteinase activity and thermotolerance of dermatophytes associated with alopecia in Uyo, Nigeria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycological research was conducted on the mycelial growth, keratinolytic proteinase activity and thermotolerance ofdermatophytes associated with alopecia patients in Uyo, Nigeria. The results revealed that Microsporum sp. - AP1, Epidermophyton sp. - AP2, Trichophyton rubrum - AP4, Trichophyton mentagrophytes - AP5 and a yeast Candida albicans - AP3 isolated exhibited variable growth and keratinase activity at different temperatures. Microsporum sp. - AP1 and T. mentagrophytes - AP5 survived heat treatment at 90 degrees C but exhibited best mycelial growth at 30 degrees C (with 53.41 mg/50 ml biomass dry weight) and 40 ...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Essien JP, Umoh AA, Akpan EJ, Eduok SI, Umoiyoho A Tags: Acta Microbiol Immunol Hung Source Type: journals

Heat resistance of dermatophyte's conidiospores from athletes kits stored in Nigerian University Sport's Center.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The incidence and heat resistance of conidiospores produced by dermatophytes isolated from athlete's kits (canvasses, stockings and spike shoes) stored in Nigerian University Sport's Centre were investigated. Epidermophyton floccosum, Microsporum oudouinii, Microsporum canis, Trichophyton concentricum, Trichophyton mentagrophytes and Trichophyton rubrum were isolated and their incidence on the athlete's kits varied with the species and type of kits. Among the isolates T. mentagrophytes, T. rubrum and E. floccosum with 25%, 23% and 20% prevalence rates respectively, were the most common isolates, and are often associate...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Essien JP, Jonah I, Umoh AA, Eduok SI, Akpan EJ, Umoiyoho A Tags: Acta Microbiol Immunol Hung Source Type: journals

Review of meningococcal infection in children at a United Kingdom hospital.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sixty-nine children were identified and evaluable. Forty-one (60%) presented with hypotension and/or abnormal neurological signs. In 34 (49%) a petechial rash was present on admission. Of note, 13 (19%) had a non-petechial rash, and rash was absent in 19 (28%). Twenty-one (30%) presented with meningism or meningitis. In one child the illness was so mild that the child was discharged prior to making a diagnosis. Five children died (7%). Sixty-three cases (91%) were diagnosed by blood or cerebrospinal fluid culture; these investigations remain the mainstay of diagnosis. PMID: 19388559 [PubMed - in process] (Source: A...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Ali S, Hovenden JL, Symon DN Tags: Acta Microbiol Immunol Hung Source Type: journals

The prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among clinical isolates from a general hospital in Iran.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted at a 900+ bed general teaching hospital, from May to September 2007, in Iran. The aim of this study was to determine the prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae and their antimicrobial pattern. The Kirby-Bauer disk diffusion method and the phenotypic disk confirmatory test were performed for each isolate. The total of 206 isolates including 106 E. coli and 100 K. pneumoniae were collected of which 122 isolates (59.2%) were ESBL positive. The prevalence of ESBL-producing strains was 59.2% (122/206). All the isolates were susceptible ...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Bazzaz BS, Naderinasab M, Mohamadpoor AH, Farshadzadeh Z, Ahmadi S, Yousefi F Tags: Acta Microbiol Immunol Hung Source Type: journals

Biotransformation of L-tyrosine to tyramine by the growing cells of Lactococcus lactis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The potential of pyridoxal-5-phosphate dependent tyrosine decarboxylase (E.C. 4.1.1.25) of Lactococcus lactis was explored for the biotransformation of L-tyrosine to tyramine. Maximum bioconversion of L-tyrosine to tyramine was achieved in tyramine production medium (pH -5.5) at 30 degrees C after 16 h of incubation with 0.2% L-tyrosine. The yield oftyramine was found to be 11.8 microg/mL by the growing cells of L. lactis at shake flask level. Growth medium and different physico-chemical parameters to maximize the biotransformation of L-tyrosine to tyramine were optimized and yielded 1.9-fold increased synthesis of tyr...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2009 Category: Microbiology Authors: Thakur M, Azmi W Tags: Acta Microbiol Immunol Hung Source Type: journals

Sixtieth anniversary of the Institute of Medical Microbiology, Semmelweis University, Budapest (1948-2008).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19130744 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Nagy K Tags: Acta Microbiol Immunol Hung Source Type: journals

Cytolytic immune lymphocytes in the armamentarium of the human host.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The author of the monograph "Cytolytic Immune Lymphocytes..." (published in 2008 by Schenk Buchverlag Campus Dialog, Budapest, Passau, Pécs) proposed several research projects and described certain clinical events that require further elaboration and documentation. In this article the author provides what is required and has since become available. The first subject matter in question concerns the fusogenic viruses. The ancient fusogenic viruses might have created the first eukaryotic cell(s) by uniting archaeabacterial and prokaryotic/protobacterial protospheroplasts. Extant fusogenic viruses either produce tumor...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Sinkovics JG Tags: Acta Microbiol Immunol Hung Source Type: journals

Emergence of antiretroviral drug resistance in therapy-naive HIV infected patients in Hungary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mutations in the HIV-1 genes associated with resistance to antiretroviral drugs were detected also in primary HIV infected individuals who did not receive antiretroviral treatment. Drug resistance genotyping of HIV pol gene was done by in situ DNA hybridization using a Line Probe Assay and by direct sequencing. Viral variants harbouring resistance mutations such as: M41, T69R, K70R, M184V, T215Y in the pol gene were detected in 14% of the subjects. HIV mutants resistant to NRT inhibitors were found in 10 and 20% of patients infected before and after the year 2000, respectively. Multiple drug resistant viruses (2-3 drug...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Juhász E, Ghidán A, Kemény B, Nagy K Tags: Acta Microbiol Immunol Hung Source Type: journals

Molecular characterisation, antibiotic sensitivity and serotyping of hungarian Streptococcus pneumoniae isolates.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we summarise the results of the first major epidemiological survey of Streptococcus pneumoniae isolates from Hungary. Neither the incidence nor the level of resistance to penicillin of the strains was high, we observed, however, 40% macrolide resistance. The main underlying mechanism was the presence of the erm(B) gene, but we could find also strains with the mef gene, some of them with unusually high resistance levels. The main serogroups in ranking order were 6, 9, 19, 23 and 14, accounting together for 88.2% of all typed strains. We found a close correlation between serotypes and resistance. As a result of...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Dobay O Tags: Acta Microbiol Immunol Hung Source Type: journals

Vancomycin resistant enterococci (VRE) still persist in slaughtered poultry in hungary 8 years after the ban on avoparcin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report we examined the glycopeptide susceptibility of enterococci, isolated in 2005, from slaughtered animals, within the confines of Hungarian Antibiotic Resistance Monitoring System. We determined the presence of the van genes as well as their genetic relatedness in enterococci from poultry. Enterococcus sp. strains (n=175) were collected from intestinal samples of slaughtered poultry in 2005. The origin of the samples was registered at county level. After screening the strains with 30 mg vancomycin disc 19 (86%) intermediate resistant and 4 (3%) fully resistant strains were found. The distribution of minimum inh...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Ghidán A, Dobay O, Kaszanyitzky EJ, Samu P, Amyes SG, Nagy K, Rozgonyi F Tags: Acta Microbiol Immunol Hung Source Type: journals

Comparison of Streptococcus mutans strains from children with caries-active, caries-free and gingivitis clinical diagnosis by pulsed-field gel electrophoresis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A study was conducted to compare the DNA structure of Streptococcus mutans strains in children with caries-active, caries-free, and gingivitis clinical diagnosis. Twenty-eight Streptococcus mutans strains from 100 children's plaques were examined by pulsed-field gel electrophoresis (PFGE) method. The classified strains were closely related to one another, though the strains originated from different disease groups. Three identical pairs were found, but the pairs in two cases belonged to different disease groups. The results of the PFGE experiments suggest that there is no correlation between the different DNA patterns ...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Herczegh A, Ghidán A, Deseo K, Kamotsay K, Tarján I Tags: Acta Microbiol Immunol Hung Source Type: journals

Seroprevalence of HSV-2 in Hungary and comparison of the HSV-2 prevalence of pregnant and infertile women.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Herpes simplex virus type 2 infection is a quite common but frequently asymptomatic, therefore undiagnosed condition. Genital HSV-2 infection may cause neonatal herpes, enhances HIV transmission and may play a role in infertility. To evaluate the prevalence of HSV-2 in Hungary we tested 2500 serum samples for the presence of anti-HSV-2 IgG by ELISA method. According to our results Hungary belongs to the low-infected countries, the HSV-2 seroprevalence grows with age and is significantly higher among women than in men. We also examined the serostatus of 512 pregnant women and 539 women attending infertility clinics. Res...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Hettmann A, Gerle B, Barcsay E, Csiszár C, Takács M Tags: Acta Microbiol Immunol Hung Source Type: journals

Expression patterns of cel5A-cel5B, two endoglucanase encoding genes of Thermobifida fusca.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Expression patterns of cel5A and cel5B, two endoglucanase encoding genes of Thermobifida fusca were compared by quantitative real-time PCR. With Avicel as carbon source the transcript level of cel5A continuously increased until the 10th hour of incubation and then a sharp decrease was observed, whereas cel5B presented a slow constitutive expression on this substrate. When the microcrystalline cellulose powder MN300 was used as the inducing carbon source, the expression patterns of the two genes were similar. A low initial level of expression was followed by a rapid increase at the 5th hour of incubation; a transient re...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Sasvári Z, Posta K, Hornok L Tags: Acta Microbiol Immunol Hung Source Type: journals

Characterisation of the first VIM metallo-beta-lactamase-producing Pseudomonas aeruginosa clinical isolate in Serbia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this is the first report of VIM MBL-producing P. aeruginosa from Serbia and also of the occurrence of such isolates belonging to the international clonal complex CC11 in this country. PMID: 19130752 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Lepsanovic Z, Libisch B, Tomanovic B, Nonkovici Z, Balogh B, Füzi M Tags: Acta Microbiol Immunol Hung Source Type: journals

Observations on environmental factors in connection with avian botulism outbreaks in a Hungarian wetland habitat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the relationship between five water-quality parameters (water temperature, pH, conductivity, water-soluble oxygen (WSO) and organic matter content (chemical oxygen demand-COD)) and avian botulism cases were examined using statistical methods. Five-five sampling points, located in high and low avian botulism risk (HR and LR) areas of Lake 'Kis-Balaton' were chosen, respectively. Data from 5 HR and 5 LR years were processed. In some cases, significant (P<0.05) differences were discovered in water temperature, pH, WSO and COD values between HR and LR sites and years. Discriminant analysis verified positive r...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2008 Category: Microbiology Authors: Babinszky G, Csitári G, Józsa S Tags: Acta Microbiol Immunol Hung Source Type: journals

Microbial lipases: at the interface of aqueous and non-aqueous media. A review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the 'working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst's preparation. An optimization process for ester synthesis from pilot scale t...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Verma ML, Azmi W, Kanwar SS Tags: Acta Microbiol Immunol Hung Source Type: journals

Effect of controlled lactic acid bacterial fermentation on the microbiological and chemical qualities of Moroccan sardines (Sardina pilchardus).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lactic acid bacteria (LAB) strains were assayed for the conservation of fresh sardine "Sardina pilchardus". Lactobacillus delbrueckii subsp. delbrueckii was used for inoculation of sardine fillets in a solution of NaCl (5%, w/w) and glucose (4%, w/w) concentration in water. Microbial counts including Standard Plate Count (SPC), LAB, yeasts, coliforms, Salmonella, staphylococci and Clostridium were followed during two weeks of storage at 30 degrees C. Determinations of chemical parameters including pH, dry matter, fat, ash, total nitrogen (NT), total volatile basic nitrogen (TVBN) and trimethylamine (TMA) were carried o...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Ndaw A, Zinedine A, Faid M, Bouseta A Tags: Acta Microbiol Immunol Hung Source Type: journals

Molecular approach and bacterial quality of drinking water of urban and rural communities in Egypt.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study indicated good quality of tap water and bottled water. The untreated water samples (hand pumps) were, however, slightly contaminated by faecal coliforms, faecal enterococci, Clostridium perfringens, Salmonella and Shigella. Consequently, the consumers in the villages receiving water through hand pumps are often exposed to the risk of water-borne diseases due to inadequate treatment of the raw water. Therefore, there are guidelines necessary to protect groundwater quality. Moreover, PCR-amplified by some functional gene fragments such as dctA, dcuB, frdA, dcuS and dcuR genes of the E. coli was adapted for use as ...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Abo-Amer AE, Soltan el-SM, Abu-Gharbia MA Tags: Acta Microbiol Immunol Hung Source Type: journals

Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc-co-DMA-cl-MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n-heptane resulted in formation of isopropyl myristate (66.0 +/- 0.3 mM) in 15 h. The reaction temperature below or higher than 65 degrees C markedly reduced the formation of isoprop...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Verma ML, Chauhan GS, Kanwar SS Tags: Acta Microbiol Immunol Hung Source Type: journals

Oral poliovirus vaccine in management of recurrent aphthous stomatitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion OPV appeared to be effective in the management of RAS. PMID: 18800598 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Yazdanpanah MJ, Mokhtari MB, Mostofi K, Soleimani M, Ebrahimirad M, Esmaili H, Ahmadi SN Tags: Acta Microbiol Immunol Hung Source Type: journals

Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns. PMID: 18800599 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2008 Category: Microbiology Authors: Erdei E, Pusztahelyi T, Miskei M, Barna T, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: journals

Novel mechanisms in nutrient activation of the yeast protein kinase A pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In yeast the Protein Kinase A (PKA) pathway can be activated by a variety of nutrients. Fermentable sugars, like glucose and sucrose, trigger a spike in the cAMP level, followed by activation of PKA and phosphorylation of target proteins causing a.o. mobilization of reserve carbohydrates, repression of stress-related genes and induction of growth-related genes. Glucose and sucrose are sensed by a G-protein coupled receptor system that activates adenylate cyclase and also activates a bypass pathway causing direct activation of PKA. Addition of other essential nutrients, like nitrogen sources or phosphate, to glucose-rep...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Thevelein JM, Bonini BM, Castermans D, Haesendonckx S, Kriel J, Louwet W, Thayumanavan P, Popova Y, Rubio-Texeira M, Schepers W, Vandormael P, Van Zeebroeck G, Verhaert P, Versele M, Voordeckers K Tags: Acta Microbiol Immunol Hung Source Type: journals

Smut fungi (Basidiomycota p.p., Ascomycota p.p.) of the world. novelties, selected examples, trends.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
After defining the meaning of the term 'smut fungus', their current taxonomic classification is presented. There are to date, 1640 'true' species of smut fungi that are classified into 2 phyla, 2 subphyla, 4 classes, 8 orders, 24 families and 90 genera. Recent changes to the classification of the smut fungi have produced some surprises when compared to their traditional classification. The variability of the symptoms produced by the smut fungi on their host plants, and the great morphological diversity of the spores is illustrated by selected pictures. Trends and perspectives in the research of the taxonomy of smut fun...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Vánky K Tags: Acta Microbiol Immunol Hung Source Type: journals

Heterotrimeric G protein mediated regulation of proteinase production in Aspergillus nidulans.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Extracellular proteinase production induced by carbon starvation was studied in a series of heterotrimeric G protein signaling pathway mutants of Aspergillus nidulans. All the mutants tested--including deltafadA (Galpha), deltasfaD (Gbeta), deltagpgA (Ggamma) and deltasfgA (regulator of FadA signaling)--showed an elevated proteinase production after glucose depletion. Our results strongly support the view that during growth, FadA/SfaD/GpgA G protein signaling inhibits proteinase production via both Galpha and Gbetagamma subunits, and all conditions, which are not sufficient to support vegetative growth and, hence, inhi...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Emri T, Szilágyi M, Justyák A, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: journals

Lactose and D-galactose catabolism in the filamentous fungus Aspergillus nidulans.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The disaccharide lactose is a byproduct of cheese production accumulating to amounts of 800,000 tons per year worldwide, of which 15% is used as a carbon source for various microbial fermentations. Nevertheless, little is known about the regulation of its metabolism in filamentous fungi. Lactose is metabolized slowly, and some important fungi such as A. niger cannot use it at all. A more detailed knowledge on the rate-limiting steps would be helpful to improve its industrial application. We have chosen A. nidulans as an object for investigating how lactose and galactose metabolism are regulated because it has long beco...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Fekete E, Padra J, Szentirmai A, Karaffa L Tags: Acta Microbiol Immunol Hung Source Type: journals

Sexual attraction: on the role of fungal pheromone/receptor systems (A review).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pheromones have been detected in all fungal phylogenetic lineages. This came as a surprise, as the general role of pheromones in mate attraction was not envisioned for some fungi. Pheromones and pheromone receptor genes have been identified, however, in members of all true fungal lineages, and even for mycelia forming organisms of plant and amoeba lineages, like oomycetes and myxomycetes. The mating systems and genes governing the mating type are different in fungi, ranging from bipolar with two opposite mating types to tetrapolar mating systems (with four possible mating outcomes, only one of which leads to fertile se...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Kothe E Tags: Acta Microbiol Immunol Hung Source Type: journals

Mold challenge study in bottled natural mineral waters and spring waters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microbiological challenge study was carried out to verify the microbial stability of bottled waters against four different mold species isolated from bottled water (Fusarium sp.; Cladosporium sp.; Penicillium chrysogenum and Aspergillus fumigatus) and to follow the growth of the molds in bottled water. Twelve types of bottled water with different mineralization and CO2 level in PET and glass packages were collected from 4 European countries. Three different inoculation levels of spore suspensions were used to contaminate bottled water samples. The surviving colony forming unit (CFU) numbers and visual growth were monit...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Pap K, Tornai-Lehoczki J, Syposs Z Tags: Acta Microbiol Immunol Hung Source Type: journals

Lignocellulolytic enzymes profile during growth and fruiting of Pleurotus ostreatus on wheat straw and tree leaves.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cultivation of two commercial Pleurotus ostreatus (oyster mushroom) strains was performed in plastic bags. Tree leaves appeared to be an excellent growth substrate for the conversion into fruiting bodies with biological efficiency of 108-118%. The level of enzyme activity was strongly regulated during the life cycle of mushrooms. However, despite the quantitative variations, each strain had a similar pattern of enzyme accumulation in fermentation of both substrates. Laccase and MnP activities were high during substrate colonization and declined rapidly during fruiting body development. On the contrary, in substrate col...
Source: Acta Microbiologica et Immunologica Hungarica - June 1, 2008 Category: Microbiology Authors: Elisashvili V, Kachlishvili E, Penninckx MJ Tags: Acta Microbiol Immunol Hung Source Type: journals

Incidence, antibiotic resistance and clonal relations of MRSA strains isolated from a Romanian university hospital.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Our aim was to estimate the frequency and characteristics ofmethicillin-resistant Staphylococcus aureus (MRSA) strains occurring in a Romanian teaching hospital. We retrospectively studied isolates from infected or colonized patients treated at the intensive care and surgical units during January 2004-December 2005. The antibiotic susceptibility of MRSA strains and the presence of mecA gene were determined. Consecutively occurring strains isolated through a three-month period were typed using pulsed field gel electrophoresis. A total of 423 S. aureus strains were identified, methicillin-resistance was detected in 211 (...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2008 Category: Microbiology Authors: Székely E, Lorinczi L, Bilca D, Fodor E, Sóki J, Sabau M Tags: Acta Microbiol Immunol Hung Source Type: journals

The novel arthritis-drug substance MCS-18 attenuates the antibody production in vivo.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DISCUSSION: These findings suggest that MCS-18 could be a potent, non-toxic antagonist or a down-regulator of TLR signalling pathway. Investigations on further models are needed to establish ifMCS-18 may influence particularly the production of RA-specific auto-antibodies, too. PMID: 18507149 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2008 Category: Microbiology Authors: Kerek F, Szegli G, Cremer L, Lupu AR, Durbaca S, Calugaru A, Herold A, Radu DL Tags: Acta Microbiol Immunol Hung Source Type: journals

Immobilization of polygalacturonase from Aspergillus niger onto activated polyethylene and its application in apple juice clarification.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present work is focused on efficient immobilization of polygalacturonase on polyethylene matrix, followed by its application in apple juice clarification. Immobilization of polygalacturonase on activated polyethylene and its use in apple juice clarification was not reported so far. Aspergillus niger Van Tieghem (MTCC 3323) produced polygalacturonase when grown in modified Riviere's medium containing pectin as single carbon source by fed-batch culture. The enzyme was precipitated with ethanol and purified by gel filtration chromatography (Sephacryl S-100) and immobilized onto glutaraldehyde-activated polyethylene. T...
Source: Acta Microbiologica et Immunologica Hungarica - March 1, 2008 Category: Microbiology Authors: Saxena S, Shukla S, Thakur A, Gupta R Tags: Acta Microbiol Immunol Hung Source Type: journals