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Gene cloning, characterization, and heterologous expression of levansucrase from Bacillus amyloliquefaciens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we cloned and characterized the enzymatic kinetics using levansucrase expressed in Escherichia coli. Optimum pH for D: -glucose production and levan formation was 6.0 and 8.0, respectively, whereas optimum temperature was 30 degrees C and 4 degrees C, respectively. The K (m) and V (max) values for levansucrase were calculated to be 47.81 mM sucrose and 57.47 mumole/min mg protein, respectively. Prominent expression of levansucrase was obtained through xylose induction in Bacillus megaterium, where most of the His(6)-tagged protein was secreted into the culture broth, giving levansucrase activity of 12,906 U/...
Source: Journal of Industrial Microbiology and Biotechnology - November 16, 2009 Category: Microbiology Authors: Rairakhwada D, Seo JW, Seo MY, Kwon O, Rhee SK, Kim CH Tags: J Ind Microbiol Biotechnol Source Type: journals

Proteolytic activity among psychrotrophic bacteria isolated from refrigerated raw milkEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Psychrotrophic bacteria were isolated from refrigerated raw milk from a processing plant in Southern Brazil. Psychrotrophic counts were between 4.9 and 7.8 log cfu/mL, and 5.3 to 7.2 log cfu/mL, for samples collected at the truck and the milk storage silo, respectively. Among the bacterial isolates, 90% were Gram-negative. Most strains presented low proteolytic activity, but strains of Burkholderia cepacia, Klebsiella oxytoca and Aeromonas sp. showed higher than 20 U/mL on azocasein as substrate. Crude proteases from selected strains were resistant to conventional heat treatments and caused coagulation of UHT milk after 5 ...
Source: International Journal of Dairy Technology - November 12, 2009 Category: Nutrition Authors: MARIA F B L NÖRNBERG, ROSANE S C FRIEDRICH, RITA D N WEISS, EDUARDO C TONDO, ADRIANO BRANDELLI Source Type: journals

Cellular components of probiotics control Yersinia ruckeri infection in rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Subcellular components of the probiotics Aeromonas sobria GC2 and Bacillus subtilis JB-1, when administered to rainbow trout, Oncorhynchus mykiss, conferred protection against a new biogroup of Yersinia ruckeri. Thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (CWPs), outer membrane proteins (OMPs), lipopolysaccharides (LPS), whole cell proteins (WCPs) and live cells followed by challenge on day 8 with Y. ruckeri led to 80[ndash]100% survival compared with 10% survival in the controls. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of WCPs and OMPs fr...
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: A Abbass, S M Sharifuzzaman, B Austin Source Type: journals

Inhibition by Chestnut Honey of N-Acyl-l-homoserine Lactones and Biofilm Formation in Erwinia carotovora, Yersinia enterocolitica, and Aeromonas hydrophilaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Journal of Agricultural and Food Chemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - November 10, 2009 Category: Food Science Tags: article Source Type: journals

Growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This paper reports the effects of changing pH (5-7) and temperature (T, 40-60 degrees C) on the efficiencies of bacterial hydrolysis of suspended organic matter (SOM) in wastewater from food waste recycling (FWR) and the changes in the bacterial community responsible for this hydrolysis. Maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at pH 5.7 and T = 44.5 degrees C. Changes in short-chain volatile organic acid profiles and in acidogenic bacterial communities were investigated under these conditions. Propionic and butyric acids concentrations increased rapidly ...
Source: Applied Microbiology and Biotechnology - November 6, 2009 Category: Microbiology Authors: Kim MD, Song M, Jo M, Shin SG, Khim JH, Hwang S Tags: Appl Microbiol Biotechnol Source Type: journals

The DC-SIGN of Zebrafish: Insights into the Existence of a CD209 Homologue in a Lower Vertebrate and Its Involvement in Adaptive Immunity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN/CD209) has become hot topic in recent studies because of its important roles in immune responses and immune escape. CD209 has been well characterized in humans and several other mammals, but little documentation exists about it in lower vertebrates. This is the first report on the identification and functional characterization of a fish DC-SIGN/CD209 molecule. The zebrafish DC-SIGN/CD209 cDNA translates into 343 aa organized into three domains structurally conserved among vertebrates. An EPN motif essential for interacting with Ca(2+) and for recognizing mann...
Source: Journal of Immunology - November 4, 2009 Category: Allergy & Immunology Authors: Lin AF, Xiang LX, Wang QL, Dong WR, Gong YF, Shao JZ Tags: J Immunol Source Type: journals

Hepatic gene expression profiling reveals protective responses in Atlantic salmon vaccinated against furunculosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Efficiency of vaccination against furunculosis depends largely on the ability of host to neutralize the negative impacts of immune responses combined with efficient clearance and prevention of tissue damages. (Source: BMC Genomics - Latest articles)
Source: BMC Genomics - Latest articles - October 30, 2009 Category: Genetics & Stem Cells Authors: Stanko SkugorSven-Martin JorgensenBjarne GjerdeAleksei Krasnov Source Type: journals

Plasmid-mediated QnrS2 determinant in an Aeromonas caviae isolate recovered from a patient with diarrhoeaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clin Microbiol Infect A qnrS2 gene was identified in an Aeromonas caviae isolate (MICs of ciprofloxacin, norfloxacin and ofloxacin >32 mg/L) from a stool sample collected from a patient with gastroenteritis. The analysis of the gyrA and parC genes revealed amino acid substitutions Ser83-Ile and Ser80-Thr, respectively. In addition, five out of 41 nalidixic acid-resistant Aeromonas isolates studied (26 identified as Aeromonas veronii bv sobria and 15 identified as A. caviae) showed ciprofloxacin resistance. The identification of plasmid-mediated qnr genes outside of the Enterobacteriaceae underlines a possible diffusion of ...
Source: Clinical Microbiology and Infection - October 28, 2009 Category: Microbiology Authors: A. Arias, C. Seral, F. Navarro, E. Miró, P. Coll, F. J. Castillo Source Type: journals

Design and Performance of a Turbot (Scophthalmus maximus) Oligo-microarray Based on ESTs from Immune Tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An expressed sequence tag database from immune tissues was used to design the first high-density turbot (Scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. Specific oligonucleotides (60 mers) were successfully designed for 2,716 out of 3,482 unique sequences of the database. An Agilent custom oligo-microarray 8 x 15 k (five replicates/gene; eight microarrays/slide) was constructed. The performance of the microarray and the sources of variation along microarray analysis were examined on spleen pools of controls and Aeromonas salmonicida-challenged fish at 3 day...
Source: Marine Biotechnology - October 20, 2009 Category: Biotechnology Authors: Millán A, Gómez-Tato A, Fernández C, Pardo BG, Alvarez-Dios JA, Calaza M, Bouza C, Vázquez M, Cabaleiro S, Martínez P Tags: Mar Biotechnol (NY) Source Type: journals

Simultaneous differential detection of human pathogenic and nonpathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The multiplex PCR based on combined gyrB and pntA provides a high discriminatory power in the differentiation between Vibrio alginolyticus and V. parahaemolyticus, and between V. cholerae and Vibrio mimicus.Significance and Impact of the Study: This assay will be useful for rapid differentiation of various Vibrio species from clinical and environmental sources and significantly overcomes the limitations of the conventional methods. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: C.S.J. Teh, K.H. Chua, K.L. Thong Source Type: journals

[Necrotizing fasciitis caused by Aeromonas hydrophila in an immunocompetent patient.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19822313 [PubMed - as supplied by publisher] (Source: Cirugia eEspanola)
Source: Cirugia eEspanola - October 9, 2009 Category: Surgery Authors: Fernández Arroyo M, González Romero D, Santana Cabrera L, Sánchez Palacios M Tags: Cir Esp Source Type: journals

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., two new clinical species from Taiwan.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two clinical Aeromonas sp. strains (A2-50 and A2-67) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on the sequences of the 16S rDNA gene that showed similarities with A. caviae, A. trota and A. aquariorum ranging from 99.6% to 99.8%. The rpoD phylogenetic tree allocated these two strains to two new and independent phylogenetic lines, the neighbouring species being A. caviae showing 93.2% similarity (56 bp differences) with strain A2-50 and 92.2% (63 bp differences) with strain A2-67. A multi-locus phylogenetic analysis of 5 housekeeping genes (gyrB, r...
Source: International Journal of Systematic and Evolutionary Microbiology - October 8, 2009 Category: Microbiology Authors: Alperi A, Martínez-Murcia AJ, Ko WC, Monera A, Saavedra MJ, Figueras MJ Tags: Int J Syst Evol Microbiol Source Type: journals

Aeromonas Sepsis as a Marker of Devitalized Tissue in Traumatic InjuriesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Surgical Infections , Vol. 0, No. 0. (Source: Surgical Infections)
Source: Surgical Infections - October 7, 2009 Category: Surgery Tags: article Source Type: journals

Detection and characterization of class 1 integrons in Aeromonas spp. isolated from human diarrheic stool in MexicoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We determined the presence of class 1 integrons related to the acquisition of resistance to antimicrobials in Aeromonas spp. isolated from individuals with diarrhea. Species were identified as A. caviae, A. hydrophila, A. veronii and A. media using PCR-RFLP of the 16S rDNA. Selected isolates were further characterized by ERIC-PCR. Resistance to chloramphenicol, aztreonam, tetracycline, trimethoprim/sulfamethoxazole, nalidixic acid and streptomycin, among others, was determined using the Kirby-Bauer method. Integrons were detected by PCR amplification of the 5[prime] conserved, variable, and 3[prime] conserved regions. Sequ...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Abigail Pérez-Valdespino, Elizabeth Fernández-Rendón, Everardo Curiel-Quesada Source Type: journals

Common Mechanistic Features among Metallo-{beta}-lactamases: A COMPUTATIONAL STUDY OF AEROMONAS HYDROPHILA CphA ENZYME [Enzyme Catalysis and Regulation]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Metallo-β-lactamases (MβLs) constitute an increasingly serious clinical threat by giving rise to β-lactam antibiotic resistance. They accommodate in their catalytic pocket one or two zinc ions, which are responsible for the hydrolysis of β-lactams. Recent x-ray studies on a member of the mono-zinc B2 MβLs, CphA from Aeromonas hydrophila, have paved the way to mechanistic studies of this important subclass, which is selective for carbapenems. Here we have used hybrid quantum mechanical/molecular mechanical methods to investigate the enzymatic hydrolysis by CphA of the antibiotic biapenem. Our calcul...
Source: Journal of Biological Chemistry - October 2, 2009 Category: Chemistry Authors: Simona, F., Magistrato, A., Dal Peraro, M., Cavalli, A., Vila, A. J., Carloni, P. Tags: Enzyme Catalysis and Regulation Source Type: journals

Aeromonas hydrophila sepsis in a preterm neonate.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19887702 [PubMed - in process] (Source: Indian Pediatrics)
Source: Indian Pediatrics - October 1, 2009 Category: Pediatrics Authors: Chaudhari T, Todd DA Tags: Indian Pediatr Source Type: journals

Integration of microfiltration and anion-exchange nanoparticles-based magnetic separation with MALDI mass spectrometry for bacterial analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is powerful in characterizing and identifying bacterial isolates. However, sufficient quantities of bacterial cells are required for generating MALDI mass spectra and a procedure to isolate and enrich target bacteria from sample matrix prior to MALDI-MS analysis is often necessary. In this paper, anion-exchange superparamagnetic nanoparticles (NPs), i.e., fluidMAG-DEAE and fluidMAG-Q, were employed to capture Aeromonas, Salmonella, Pseudomonas, Enterococcus, Bacillus, Staphylococcus and Escherichia coli from aqueous solutions and fresh water. The ...
Source: Talanta - September 30, 2009 Category: Chemistry Authors: Li S, Guo Z, Liu Y, Yang Z, Hui HK Tags: Talanta Source Type: journals

Structural Basis for the Kexin-like Serine Protease from Aeromonas sobria as Sepsis-causing Factor [Protein Structure and Folding]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The anaerobic bacterium Aeromonas sobria is known to cause potentially lethal septic shock. We recently proposed that A. sobria serine protease (ASP) is a sepsis-related factor that induces vascular leakage, reductions in blood pressure via kinin release, and clotting via activation of prothrombin. ASP preferentially cleaves peptide bonds that follow dibasic amino acid residues, as do Kex2 (Saccharomyces cerevisiae serine protease) and furin, which are representative kexin family proteases. Here, we revealed the crystal structure of ASP at 1.65 Å resolution using the multiple isomorphous replacement method with anoma...
Source: Journal of Biological Chemistry - September 24, 2009 Category: Chemistry Authors: Kobayashi, H., Utsunomiya, H., Yamanaka, H., Sei, Y., Katunuma, N., Okamoto, K., Tsuge, H. Tags: Protein Structure and Folding Source Type: journals

Effect of oral immunization with Aeromonas hydrophila ghosts on protection against experimental fish infectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Oral immunization with A. hydrophila ghosts can elicit systemic and mucosal adaptive immune responses and has higher potential to induce protective adaptive immunity than normal vaccine.Significance and Impact of the Study: Oral immunization with bacterial ghosts is a promising new solution with potential application to prevent diseases in fish. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 22, 2009 Category: Microbiology Authors: F.P. Tu, W.H. Chu, X.Y. Zhuang, C.P. Lu Source Type: journals

A novel method for the diagnosis of drowning by detection of Aeromonas sobria with PCR methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, from the preserved blood samples of 32 freshwater drowning cases, specific DNA fragments of Aeromonas sobria, one of the most common aquatic bacteria, were examined using PCR. The DNA fragments of the bacterium were detected from 27 of 32 cases with first round PCR or nested-PCR. The remaining 5 cases in which bacterial DNA was not detected had longer storage periods for the blood samples and shorter time intervals from drowning to death. These results indicate that the present method can be applied to the diagnosis of death by drowning. (Source: Legal Medicine)
Source: Legal Medicine - September 18, 2009 Category: Forensic Medicine Authors: Miwako Aoyagi, Kimiharu Iwadate, Kenji Fukui, Shuntaro Abe, Kentaro Sakai, Kyoko Maebashi, Eriko Ochiai, Mihoko Nakamura Tags: Original Articles Source Type: journals

Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146bp and contained a 672bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif, a potential N-linked glycosylation site and ten cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to d...
Source: Developmental and Comparative Immunology - September 8, 2009 Category: Allergy & Immunology Authors: Zhou X, Feng H, Guo Q, Dai H Tags: Dev Comp Immunol Source Type: journals

Gene Cloning, Expression, and Characterization of Recombinant Aerolysin from Aeromonas hydrophila.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aerolysin is a significant virulent toxin protein secreted by Aeromonas hydrophila; it produces deep wound infections and hemorrhagic septicemia. The complete aerolysin gene (1,482 bp) was amplified from A. hydrophila. Furthermore, it was cloned and expressed into Escherichia coli BL21(DE3) codon plus RP cells using 0.5 mM IPTG for induction. The protein size was 54 kDa as estimated by SDS-PAGE, and it was purified by Ni-NTA affinity chromatography. Anti-His antibodies were used to characterize the expressed aerolysin by Western blotting and showed hemolytic activity with fish red blood cells. Aerolysin may be used as ...
Source: Applied Biochemistry and Biotechnology - September 7, 2009 Category: Biochemistry Authors: Singh V, Somvanshi P, Rathore G, Kapoor D, Mishra BN Tags: Appl Biochem Biotechnol Source Type: journals

N-Acyl homoserine lactones involved in quorum sensing control type VI secretion system, biofilm formation, protease production, and in vivo virulence from a clinical isolate of Aeromonas hydrophila.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we delineated the role of N-acyl homoserine lactone(s) (AHLs) mediated quorum sensing (QS) in the virulence of diarrheal isolate SSU of A. hydrophila by generating a double knockout DeltaahyRI mutant. The protease production was substantially reduced in the DeltaahyRI mutant when compared to that in the wild-type (WT) strain. Importantly, based on Western blot analysis, the DeltaahyRI mutant was unable to secrete type VI secretion system-associated effectors, namely hemolysin-co-regulated protein and the valine-glycine repeat family of proteins, while significant levels of these effectors were detected in th...
Source: Microbiology - September 2, 2009 Category: Microbiology Authors: Khajanchi BK, Sha J, Kozlova EV, Erova TE, Suarez G, Sierra JC, Popov VL, Horneman AJ, Chopra AK Tags: Microbiology Source Type: journals

An experimental vaccine against Aeromonas hydrophila can induce protection in rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A candidate vaccine against Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss, was developed using a bacterial lysate. To test the strength of protection, A. hydrophila challenge models were compared using injection into both the intraperitoneal (IP) cavity and the dorsal sinus (DS) with selected doses of live bacteria washed in saline or left untreated. Unlike the IP route, injection into the DS with either saline washed or unwashed cells resulted in consistent cumulative mortality and a dose response that could be used to establish a standard challenge having an LD50 of approximately 3 × 107 colony forming unit...
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: S E LaPatra, K P Plant, S Alcorn, V Ostland, J Winton Source Type: journals

Occurrence of motile Aeromonas in municipal drinking water and distribution of genes encoding virulence factors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aeromonas-associated cases of gastroenteritis are generally considered waterborne. The purpose of this study was to evaluate the potential microbiological risk associated with the presence of these bacteria in public drinking water. Over a period of one year, 132 drinking-water samples were monitored in León (NW of Spain, 137,000 inhabitants) for mandatory drinking-water standards and the occurrence of Aeromonas spp. Samples were taken at the municipal water treatment plant, one storage facility, and two public artesian drinking-water fountains. Because of low numbers of coliforms or Clostridium perfringens, the n...
Source: International Journal of Food Microbiology - August 21, 2009 Category: Food Science Authors: Pablos M, Rodríguez-Calleja JM, Santos JA, Otero A, García-López ML Tags: Int J Food Microbiol Source Type: journals

Management of human and animal bite wound infection: An overviewEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Animal and human bite wounds can lead to serious infections. The organisms recovered generally originate from the biter’s oral cavity and the victim’s skin flora. Anaerobes were isolated from more than two thirds of human and animal bite infections. Streptococcus pyogenes is often recovered in human bites, Pasteurella multocida in animal bites, Eikenella corrodens in animal and human, Capnocytophaga spp, Neisseria weaveri, Weeksella zoohelcum, Neisseria canis, Staphylococcus intermedius, nonoxidizer-1, and eugonic oxidizer-2 in dog, Flavobacterium group in pig, and Actinobacillus spp in horse and ...
Source: Current Infectious Disease Reports - August 17, 2009 Category: Infectious Diseases Tags: Current Infectious Disease Reports Source Type: journals

Identification, Biosynthesis, and Characterization of Polyhydroxyalkanoate Copolymer Consisting of 3-Hydroxybutyrate and 3-Hydroxy-4-methylvalerate.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found that Ralstonia eutropha strain PHB(-)4 expressing the polyhydroxyalkanoate (PHA) synthase 1 (PhaC1(Ps)) from Pseudomonas sp. 61-3 synthesized a PHA copolymer containing a 3-hydroxybutyrate (3HB) and small amounts of 3-hydroxy-4-methylvalerate (3H4MV) and 3-hydroxyvalerate (3HV) from fructose as a sole carbon source. 3H4MV is a monomer unit that has hitherto not been reported as a naturally occurring component of PHAs. To increase the 3H4MV fraction in PHA copolymers, the culture medium was supplemented with four structural analogs that served as 3H4MV precursors. Of these, 4-methylvalerate (4MV) was...
Source: Biomacromolecules - August 13, 2009 Category: Biochemistry Authors: Tanadchangsaeng N, Kitagawa A, Yamamoto T, Abe H, Tsuge T Tags: Biomacromolecules Source Type: journals

The Aeromonas hydrophila AH-3 T3SS expression: a study of its regulatory network.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we analysed the expression of the T3SS (type 3 secretion) regulon by analysing the activity of the aopN-aopD and aexT promoters (T3SS machinery components and effector, respectively) by means of two different techniques: promoterless-gfp fusions and Real Time PCR. The expression of A. hydrophila AH-3 T3SS regulon was induced in response to several environmental factors, of which calcium depletion, high magnesium concentration and high growth temperature were shown to be the major ones. Once the optimal conditions were established, we tested the expression of the T3SS regulon in the background of several viru...
Source: Applied and Environmental Microbiology - August 13, 2009 Category: Microbiology Authors: Vilches S, Jimenez N, Tomás JM, Merino S Tags: Appl Environ Microbiol Source Type: journals

[Leech-borne infection on a TRAM flap: A case report.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a new case of delayed leech-borne infection in mammary reconstruction by a Transverse Rectus Abdominis Myocutaneous flap (TRAM) flap, which caused the flap loss. The use of prophylactic antibiotics is a way to prevent A.hydrophila infection (third generation cephalosporin, ciprofloxacin). This antibioprophylaxy must be followed until wound closure of the venous congested tissue. PMID: 19674823 [PubMed - as supplied by publisher] (Source: Annales de Chirurgie Plastique et Esthetique)
Source: Annales de Chirurgie Plastique et Esthetique - August 9, 2009 Category: Cosmetic Surgery Authors: Bourdais L, Heusse JL, Aillet S, Schoentgen C, Watier E Tags: Ann Chir Plast Esthet Source Type: journals

The structure of the di-zinc subclass B2 metallo-{beta}-lactamase CphA reveals that the second inhibitory zinc ion binds in the "histidine" site.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacteria can defend themselves against beta-lactam antibiotics through the expression of class B beta-lactamases, which cleave the beta-lactam amide bond and render the molecule harmless. There are three subclasses of class B beta-lactamases (B1, B2 and B3), all of which require Zn(2+) for activity and can bind either one or two zinc ions. Whereas the B1 and B3 metallo-beta-lactamases are most active as di-zinc enzymes, subclass B2 enzymes such as Aeromonas hydrophila CphA are inhibited by the binding of a second zinc ion. We crystallized A. hydrophila CphA in order to determine the binding site of the inhibitory zinc ...
Source: Antimicrobial Agents and Chemotherapy - August 2, 2009 Category: Microbiology Authors: Bebrone C, Delbrück H, Kupper MB, Schlömer P, Willmann C, Frère JM, Fischer R, Galleni M, Hoffmann KM Tags: Antimicrob Agents Chemother Source Type: journals

Aeromonas fluvialis sp. nov., isolated from a Spanish river.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A Gram-negative, facultatively anaerobic bacterial strain designated 717 was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being A. veronii (99.5% similarity, with 7 different nucleotides). A polyphasic study based on a Multilocus Phylogenetic Analysis of 5 housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684bp) showed isolate 717 as an independent phylogenetic line within the genus Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila...
Source: International Journal of Systematic and Evolutionary Microbiology - July 30, 2009 Category: Microbiology Authors: Alperi A, Martínez-Murcia AJ, Monera A, Saavera MJ, Figueras MJ Tags: Int J Syst Evol Microbiol Source Type: journals

Isolation of faecal coliform bacteria from the American alligator (Alligator mississippiensis)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: A prevalence of enteric bacteria, both faecal coliforms and potential pathogens, was observed in American alligators. The high faecal coliform bacterial density of alligator faeces may suggest that alligators are a potential source of bacterial contamination in South Carolina coastal waters.Significance and Impact of the Study: These findings help to increase our understanding of faecal coliform and potential pathogenic bacteria from poikilothermic reptilian sources, as there is the potential for these sources to raise bacterial water quality levels above regulatory thresholds. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - July 24, 2009 Category: Microbiology Authors: M.A. Johnston, D.E. Porter, G.I. Scott, W.E. Rhodes, L.F. Webster Source Type: journals

Divergent evolution and purifying selection of the flaA gene sequences in AeromonasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The models applied to our set of sequences allowed us to determine the possible evolutionary pathway followed by the flaA gene in Aeromonas, suggesting that this gene have probably been evolving independently in the two groups of Aeromonas species since the divergence of a distant common ancestor after one or several episodes of positive selection.ReviewersThis article was reviewed by Alexey Kondrashov, John Logsdon and Olivier Tenaillon (nominated by Laurence D Hurst). (Source: Biology Direct)
Source: Biology Direct - July 20, 2009 Category: Biology Authors: Maribel FarfanDavid Minana-GalbisM Carmen FusteJ Gaspar Loren Source Type: journals

Exploring threshold operation criteria of biostimulation for azo dye decolorization using immobilized cell systems.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This follow-up study provided an evaluation on threshold operation criteria of biostimulation in immobilized cell systems (ICSs) with Aeromonas hydrophila onto packing materials Porites corals. Essential nutrients in appropriate flow rate for biostimulation were inevitably required to maintain maximum attached cell population for cost-effective biodecolorization. With the method of "graphical reconstruction", the most economically feasible strategy of medium stimulation for color removal was quantitatively revealed. Our findings pointed out no matter what operation mode of reactor was (e.g., suspended batch cultures or...
Source: Bioresource Technology - July 20, 2009 Category: Biotechnology Authors: Chen BY, Yen CY, Chen WM, Chang CT, Wang CT, Hu YC Tags: Bioresour Technol Source Type: journals

Antimicrobial resistance and in vitro gene transfer in bacteria isolated from the ulcers of EUS-affected fish in IndiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The resistance to ampicillin, erythromycin, sulfadiazine, novobiocin and chloramphenicol is prevalent among the bacteria isolated from EUS-affected fish, and resistant determinants of some of these antibiotics have been transferred to the bacteria of other origin.Significance and Impact of the Study: The emergence of antibiotic resistance bacteria and gene transfer in vitro suggests that antibiotics should be used more cautiously to treat Aeromonas infections in aquaculture. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - July 20, 2009 Category: Microbiology Authors: A. Das, D. Saha, J. Pal Source Type: journals

Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonadsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Twelve strains of fish pathogenic aeromonads were identified by 16S rRNA sequencing as Aeromonas bestiarum, A. hydrophila, A. hydrophila subsp. dhakensis, A. salmonicida subsp. salmonicida, A. sobria biovar sobria and A. veronii biovar sobria. Following intramuscular injection, A. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. Extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. Congo red absorption and siderophore production was recorded, but not so the suicide phenomenon or slime production. Sodium dodecyl su...
Source: Journal of Fish Diseases - July 9, 2009 Category: Zoology Authors: P Orozova, M Barker, D A Austin, B Austin Source Type: journals

In vivo modulation of immune response and antioxidant defense in Atlantic cod, Gadus morhua following oral administration of oxolinic acid and florfenicol.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxolinic acid and florfenicol are the commonly used antibiotics for the treatment of bacterial diseases in Atlantic cod, Gadus morhua. The changes in selected innate humoral immune response of the fish, bacterial proliferation in serum and transcriptional activity of selected immune- and antioxidant defense-related genes following oral administration of these antimicrobial compounds were evaluated. Juvenile cod (75-100g) were fed commercial feed coated with either florfenicol (10mg kg(-1) fish, active ingredient) or oxolinic acid (20mg kg(-1) fish, active ingredient) at a ration of 0.5% body weight for 10 days. Whole b...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - July 9, 2009 Category: Biochemistry Authors: Caipang CM, Lazado CC, Brinchmann MF, Berg I, Kiron V Tags: Comp Biochem Physiol C Toxicol Pharmacol Source Type: journals

Use of bacterial lipopolysaccharide (LPS) as an immunostimulant for the control of Aeromonas hydrophila infections in rainbow trout Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: LPS was effective at preventing disease caused by A. hydrophila and in stimulating the innate immune response of rainbow trout.Significance and Impact of the Study: The results of this study highlight the role of LPS in fish disease control. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - July 6, 2009 Category: Microbiology Authors: E.J. Nya, B. Austin Source Type: journals

Characterization of electrochemical activity of a strain ISO2-3 phylogenetically related to Aeromonas sp. isolated from a glucose-fed microbial fuel cellEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The microbial communities associated with electrodes in closed and open circuit microbial fuel cells (MFCs) fed with glucose were analyzed by 16S rRNA approach and compared. The comparison revealed that bacteria affiliated with the Aeromonas sp. within the Gammaproteobacteria constituted the major population in the closed circuit MFC (harvesting electricity) and considered to play important roles in current generation. We, therefore, attempted to isolate the dominant bacteria from the anode biofilm, successfully isolated a Fe (III)-reducing bacterium phylogenetically related to Aeromonas sp. and designated as strain ISO2-3...
Source: Biotechnology and Bioengineering - July 1, 2009 Category: Biomedical Science Authors: Kyungmi Chung, Satoshi Okabe Source Type: journals

Phylogenetic analysis and identification of Aeromonas species based on sequencing of the cpn60 universal target.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, cpn60 UT sequencing was shown to be a universal, useful, simple and rapid method for the identification and phylogenetic affiliation of Aeromonas strains. PMID: 19567585 [PubMed - as supplied by publisher] (Source: International Journal of Systematic and Evolutionary Microbiology)
Source: International Journal of Systematic and Evolutionary Microbiology - June 29, 2009 Category: Microbiology Authors: Miñana-Galbis D, Urbizu-Serrano A, Farfán M, Fusté MC, Loren JG Tags: Int J Syst Evol Microbiol Source Type: journals

PCR–DGGE-based methodologies to assess diversity and dynamics of Aeromonas communitiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The three systems used showed to be useful to describe the diversity of Aeromonas communities. However, the combined use of more than one primer set is advisable.Significance and Impact of the Study: The methods presented here can be applied to understand the natural pool of Aeromonas and also to monitor and control these bacteria in aquatic reservoirs. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - June 29, 2009 Category: Microbiology Authors: V. Calhau, M. Tacão, F. Morgado, A. Correia, I. Henriques Source Type: journals

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188bp and contained a 312bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constit...
Source: Developmental and Comparative Immunology - June 27, 2009 Category: Allergy & Immunology Authors: Zhou X, Guo Q, Dai H Tags: Dev Comp Immunol Source Type: journals

Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from Aeromonas salmonicida subsp. achromogenesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two mutants of the toxic extracellular zinc endopeptidase AsaP1 (AsaP1_E294Q and AsaP1_E294A) of Aeromonas salmonicida subsp. achromogenes were expressed in Escherichia coli and crystallized by the vapour-diffusion method. Crystals were obtained using several precipitants and different protein concentrations. Protein crystals were found in a monoclinic (C2) as well as an orthorhombic (P212121) space group. The crystals belonging to the monoclinic space group C2 had unit-cell parameters a = 103.4, b = 70.9, c = 54.9 Å, β = 109.3° for AsaP1_E294A, and a = 98.5, b = 74.5, c = 54.7 Å, β = 112.4° for AsaP1_E294Q. ...
Source: Acta Crystallographica Section F - June 26, 2009 Category: Biochemistry Authors: Bogdanović, X.Singh, R.K.Hentschke, J.Gudmundsdóttir, B.K.Hinrichs, W. Tags: zinc metallopeptidases Aeromonas salmonicida subsp. achromogenes crystallization communications Source Type: journals

Effect of Lactococcus lactis CLFP 100 and Leuconostoc mesenteroides CLFP 196 on Aeromonas salmonicida Infection in Brown Trout (Salmo trutta)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
J Mol Microbiol Biotechnol (DOI:10.1159/000226588) (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - June 25, 2009 Category: Microbiology Source Type: journals

Occurrence of heavy metals and antibiotic resistance in bacteria from internal organs of american bullfrog (Rana catesbeiana) raised in MalaysiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A total of 40 bacteria have been successfully isolated from internal organs of the American bullfrog (Rana catesbeiana) raised in Malaysia, namely, eight isolates of Aeromonas spp., 21 of Edwardsiella spp., six of Flavobacterium spp. and five of Vibrio spp. In terms of antibiotic susceptibility testing, each isolate was tested against 21 antibiotics, resulting in 482 (57.3%) cases of sensitivity and 61 (7.3%) cases of partial sensitivity. Meanwhile, 297 (35.4%) bacterial isolates were registered as resistant. The multiple antibiotic resistance (MAR) index of each bacterial species indicated that bacteria from raised bullfr...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 25, 2009 Category: Tropical Medicine Source Type: journals

Infections in Patients With Aplastic AnemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Infection is a major cause of death in patients with aplastic anemia (AA). There are differences between the immunocompromised state of a patient with AA and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. Prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. Recovery from neutropenia is directly related to survival, and supportive care plays a large role in protection while the patient is in a neutropenic state. The most common invasive mycoses include the Aspergillus species, Z...
Source: Seminars in Hematology - June 25, 2009 Category: Hematology Authors: Jessica M. Valdez, Phillip Scheinberg, Neal S. Young, Thomas J. Walsh Source Type: journals

Prevalence of Genes Encoding the Type Three Secretion System and the Effectors AexT and AexU in the Aeromonas veronii GroupEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DNA and Cell Biology , Vol. 0, No. 0. (Source: DNA and Cell Biology)
Source: DNA and Cell Biology - June 17, 2009 Category: Cytology Tags: article Source Type: journals

New Structural Variants of Homoserine Lactones in Bacteria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we describe for the first time the occurrence of acyl chains carrying a methyl branch, and present a GC-MS-based method that can be used to distinguish these compounds from unbranched isomers. The bacterium Aeromonas culicicola produces several methyl branched AHLs. In Jannaschia helgolandensis-a marine bacterium of the Roseobacter clade-a doubly unsaturated AHL, (2E,9Z)-N-(2,9-hexadecadienoyl)-L-homoserine lactone, occurs. The location and configuration of the double bonds was proven by spectrometric investigation and synthesis. Finally, a method was developed to establish the absolute configuration of 3-hyd...
Source: Chembiochem - June 15, 2009 Category: Biochemistry Authors: Thiel V, Kunze B, Verma P, Wagner-Döbler I, Schulz S Tags: Chembiochem Source Type: journals

Inhibition of frog antimicrobial peptides by extracellular products of the bacterial pathogen Aeromonas hydrophilaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The proteolytic ECPs of Aer. hydrophila have the ability to inhibit the skin antimicrobial peptides of frogs.Significance and Impact of the Study: The results of this study provide new information on the association of ECPs with the resistance of Aer. hydrophila to frog antimicrobial peptides. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - June 14, 2009 Category: Microbiology Authors: E. Schadich, A.L.J. Cole Source Type: journals

Quantitative analysis of bacterial adhesion to fish tissue.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Adhesion to host tissue represents a first crucial step in most bacterial infections. Both specific adhesion-ligand as well as hydrophobic interactions may be involved. The adhesion of Aeromonas salmonicida subsp. salmonicida, Lactococcus garvieae, and Yersinia ruckeri strains to fish tissue cells was assessed. To determine whether the observed bacterial adhesion to fish tissue cells was caused by non-specific interactions, adhesion to bovine serum albumin (BSA) and polystyrene was also tested. Our results demonstrated that non-specific adhesion such as hydrophobic interactions are only partially involved in the bindin...
Source: Colloids and Surfaces - June 13, 2009 Category: Biotechnology Authors: Vendrell D, Balcázar JL, Calvo AC, de Blas I, Ruiz-Zarzuela I, Gironés O, Múzquiz JL Tags: Colloids Surf B Biointerfaces Source Type: journals