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Am J Physiol Cell Physiol; +26 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
26 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/11/18PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - November 18, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +31 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
31 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/10/06PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - October 7, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +26 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
26 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/09/23PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - September 24, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +42 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
42 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/08/20PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - August 20, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +29 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
29 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/07/10PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - July 10, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +155 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
155 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2009/06/27PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - June 27, 2009 Category: Cytology Tags: Report Source Type: journals

Inhibitory Effects of Polyunsaturated Fatty Acids on Kv4/KChIP Potassium Channels.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Kv4/KChIP potassium channels are a major class of rapidly inactivating K(+) channels in neurons and cardiac muscle. Modulation of Kv4/KChIP channels by polyunsaturated fatty acids (PUFAs) is important in the regulation of cellular excitability and the induction of activity-dependent synaptic plasticity. Using the Xenopus oocyte expression system, we studied the inhibition by PUFAs of the peak outward K(+) current and the accompanying increase in the rate of current inactivation of rKv4.2/rKChIP1b. Inhibitory effects do not depend on KChIP co-expression since Kv4.2 channels lacking an N-terminal KChIP association region...
Source: Am J Physiol Cell Ph... - March 4, 2009 Category: Cytology Authors: Boland LM, Drzewiecki MM, Timoney G, Casey E Tags: Am J Physiol Cell Physiol Source Type: journals

Dynamic interactions between L-type voltage sensitive calcium channel (VSCC) Cav1.2 ({alpha}1C) subunits and ahnak in osteoblastic cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Voltage sensitive calcium channels (VSCCs) mediate Ca(2+) permeability in osteoblasts. Association between VSCC alpha1 and beta subunits targets channel complexes to the plasma membrane and modulates function. In mechanosensitive tissues, a 700kDa ahnak protein anchors VSCCs to the actin cytoskeleton, via the beta2 subunit of the L-type Cav1.2 (alpha1C) VSCC complex. Cav1.2 is the major alpha1 subunit in osteoblasts, but the cytoskeletal complex and subunit composition are unknown. Among the four beta subtypes, beta2 and to a lesser extent beta3 subunits, co-immunoprecipitated with the Cav1.2 subunit in MC3T3-E1 preost...
Source: Am J Physiol Cell Ph... - March 4, 2009 Category: Cytology Authors: Shao Y, Czymmek KJ, Jones PA, Fomin VP, Akanbi K, Duncan RL, Farach-Carson MC Tags: Am J Physiol Cell Physiol Source Type: journals

HL-1 mouse cardiomyocyte injury and death after simulated ischemia and reperfusion: roles of pH, Ca2+-independent phospholipase A2, and Na+/H+ exchange.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Ca(2+)-independent phospholipase A2 group VI (PLA2-VI) and the Na(+)/H(+) exchanger isoform 1 (NHE1) are highly pH sensitive proteins which exert both protective and detrimental effects in cardiac ischemia/reperfusion. Here, we investigated the role of pHo in ischemia/reperfusion injury and death, and in regulation and function of iPLA2-VI and NHE1 under these conditions. HL-1 cardiomyocytes were exposed to simulated ischemia (SI, 0.5% O2, 8 mM K(+), 20 mM lactate) at pHo 6.0 and 7.4, with or without 4 or 8 h of reperfusion (SI/R). Cytochrome c release and caspase-3 activation were reduced after acidic compared to ...
Source: Am J Physiol Cell Ph... - March 4, 2009 Category: Cytology Authors: Andersen AD, Poulsen KA, Lambert IH, Pedersen SF Tags: Am J Physiol Cell Physiol Source Type: journals

A rapid, PPARgamma-dependent, effect of pioglitazone on phosphorylation of myosin phosphatase targeting subunit - MYPT.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the effect of pioglitazone (PIO - 20microM) in rat primary cultured aortic smooth muscle cells on constitutive phosphorylation of the regulatory subunit (MYPT) of myosin phosphatase. PIO decreased phosphoyrlation of Thr697 on MYPT within 15 min. and the inhibition was maintained up to 6 hours. The PPARgamma antagonist GW9662 (5microM) abrogated the inhibition of Thr697 phosphorylation mediated by PIO. Because longer term PIO treatment inhibits RhoA/Rho-kinase signaling and Thr697 phosphorylation we tested the effect of the Rho-kinase inhibitor Y27632 (10microM) on the inhibition of Thr697 phosphorylation by PIO...
Source: Am J Physiol Cell Ph... - March 4, 2009 Category: Cytology Authors: Atkins KB, Irey B, Xiang N, Brosius 3rd FC Tags: Am J Physiol Cell Physiol Source Type: journals

Directed differentiation of mouse cochlear neural progenitors in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, a...
Source: Am J Physiol Cell Ph... - March 1, 2009 Category: Cytology Authors: Lin J, Feng L, Hamajima Y, Komori M, Burns TC, Fukudome S, Anderson J, Wang D, Verfaillie CM, Low WC Tags: Am J Physiol Cell Physiol Source Type: journals

Am J Physiol Cell Physiol; +28 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
28 new PubMed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These PubMed results were generated on 2009/02/28PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - February 28, 2009 Category: Cytology Tags: Report Source Type: journals

Am J Physiol Cell Physiol; +21 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
21 new PubMed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These PubMed results were generated on 2009/02/22PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - February 22, 2009 Category: Cytology Tags: Report Source Type: journals

Determination of apparent calcium affinity for endogenously expressed human sarco/endoplasmic reticulum calcium ATPase isoform, SERCA3.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the properties of endogenous human SERCA3 and SERCA2b using a fluorescence-based assay for monitoring continuous Ca(2+) uptake into microsomes. The kinetic parameters were derived using a cooperative two-component uptake model for Ca(2+) activation, and the values assigned to SERCA3 were confirmed using the highly specific human SERCA3 inhibitory antibody, PL/IM430. First, using recombinant human SERCA3 and SERCA2b proteins transiently expressed in HEK-293 cells, we confirmed the previously observed low apparent Ca(2+) affinity for SERCA3 compared to SERCA2b (1.10 +/- 0.04 microM versus 0.26 +/- 0.01 microM, re...
Source: Am J Physiol Cell Ph... - February 18, 2009 Category: Cytology Authors: Chandrasekera PC, Kargacin ME, Deans JP, Lytton J Tags: Am J Physiol Cell Physiol Source Type: journals

Preadipocyte apoptosis is prevented by macrophage-conditioned medium in a PDGF-dependent manner.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Obesity is associated with macrophage accumulation and inflammation in adipose tissue. Macrophage-secreted factors have been reported to inhibit the differentiation of preadipocytes into adipocytes and to modulate adipogenic extracellular matrix gene expression. To enlarge our understanding of macrophages and the scope of their interactions with preadipocytes, we investigated their effect on preadipocyte survival. Acute exposure of 3T3-L1 preadipocytes to J774A.1 macrophage-conditioned medium (MacCM) stimulated platelet-derived growth factor receptor (PDGFR) tyrosine phosphorylation by 4.1-fold. There were significant ...
Source: Am J Physiol Cell Ph... - February 18, 2009 Category: Cytology Authors: Molgat A, Gagnon A, Sorisky A Tags: Am J Physiol Cell Physiol Source Type: journals

Sequential effects of GSNO and H2O2 on the Ca2+-sensitivity of the contractile apparatus of fast- and slow-twitch skeletal muscle fibers from the rat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) and nitric oxide (NO), have been shown to differentially alter the Ca(2+)-sensitivity of the contractile apparatus of fast-twitch skeletal muscle leading to the proposal that normal muscle function is controlled by perturbations in the amounts of these two groups of molecules (28). However, no previous studies have examined if these opposing actions are retained when the contractile apparatus is subjected to both molecule types. Using mechanically skinned fast- and slow-twitch skeletal muscle fibers of the rat, we compared the effects of sequential additio...
Source: Am J Physiol Cell Ph... - February 18, 2009 Category: Cytology Authors: Spencer T, Posterino GS Tags: Am J Physiol Cell Physiol Source Type: journals

Angiotensin II inhibits the Na+-K+ pump via PKC dependent activation of NADPH oxidase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The sarcolemmal Na(+)-K(+) pump, pivotal in cardiac myocyte function, is inhibited by angiotensin II (Ang II). Since Ang II activates NADPH oxidase we tested the hypothesis that NADPH oxidase mediates the pump inhibition. Exposure to 100 nmol/L Ang II increased superoxide-sensitive fluorescence of isolated rabbit ventricular myocytes. The increase was abolished by pegylated superoxide dismutase (SOD); by the NADPH oxidase inhibitor apocynin; and by myristolated inhibitory peptide to epsilon-protein kinase C (epsilonPKC), previously implicated in Ang II-induced Na(+)-K(+) pump inhibition. A role for epsilonPKC was also ...
Source: Am J Physiol Cell Ph... - February 4, 2009 Category: Cytology Authors: White CN, Figtree GA, Liu CC, Garcia A, Hamilton EJ, Chia KK, Rasmussen HH Tags: Am J Physiol Cell Physiol Source Type: journals

The nitric oxide-mediated protection of endothelial cells from hydrogen peroxide is mediated by intracellular zinc and glutathione.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxidative stress may cause endothelial dysfunction and vascular disease. It has been shown that NO protects endothelial cells (EC) against H2O2-induced toxicity. In addition, it is known that NO within cells induces a zinc release from proteins containing zinc sulfur complexes. The aim of this study was to investigate whether zinc released intracellularly by NO plays a signaling role in the NO-mediated protection against H2O2 in rat aortic EC. Our results show that the NO-mediated protection towards H2O2 depends on the activities of glutathione peroxidase and glutamate cysteine ligase (GCL), the rate-limiting enzyme of...
Source: Am J Physiol Cell Ph... - February 4, 2009 Category: Cytology Authors: Cortese-Krott MM, Suschek CV, Wetzel W, Kröncke KD, Kolb-Bachofen V Tags: Am J Physiol Cell Physiol Source Type: journals

Autocrine atp release coupled to extracellular pyrophosphate accumulation in vascular smooth muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
AUTOCRINE ATP RELEASE COUPLED TO EXTRACELLULAR PYROPHOSPHATE ACCUMULATION IN VASCULAR SMOOTH MUSCLE CELLS. Am J Physiol Cell Physiol. 2009 Feb 4; Authors: Prosdocimo DA, Douglas DC, Romani A, O'Neill WC, Dubyak GR Extracellular pyrophosphate (PPi) is a potent suppressor of physiological calcification in bone and pathological calcification in blood vessels. Ecto-nucleotide pyrophosphatase/ phosphodiesterases (eNPPs) generate PPi via the hydrolysis of ATP released into extracellular compartments by poorly understood mechanisms. Here we report that cultured vascular smooth muscle cells (VSMC) from rat aorta genera...
Source: Am J Physiol Cell Ph... - February 4, 2009 Category: Cytology Authors: Prosdocimo DA, Douglas DC, Romani A, O'Neill WC, Dubyak GR Tags: Am J Physiol Cell Physiol Source Type: journals

Fibrinogen-induced Endothelin-1 Production from Endothelial Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously demonstrated that fibrinogen (Fg) binding to the vascular endothelial intercellular adhesion molecule-1 (ICAM-1) leads to microvascular constriction in vivo and in vitro. Although a role of endothelin-1 (ET-1) in this Fg-induced vasoconstriction was suggested, the mechanism of action was not clear. In the current study, we tested the hypothesis that Fg-induced vasoconstriction results from ET-1 production by vascular endothelial cells (EC) and is mediated by activation of extracellular signal-regulated kinase -1/2 (ERK-1/2). Confluent, rat heart microvascular endothelial cells (RHMECs) were treated with o...
Source: Am J Physiol Cell Ph... - February 4, 2009 Category: Cytology Authors: Sen U, Tyagi N, Patibandla PK, Dean WL, Tyagi SC, Roberts AM, Lominadze D Tags: Am J Physiol Cell Physiol Source Type: journals

Attenuation of Proliferation and Migration of Retinal Pericytes in the Absence of Thrombospondin-1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Perivascular supporting cells, including vascular smooth muscle cells (VSMC) and pericytes (PC), provide instructive signals to adjacent endothelial cells (EC) helping to maintain vascular homeostasis. These signals are provided through direct contact and by release of soluble factors by these cells. Thrombospondin-1 (TSP1) is a matricellular protein and an autocrine factor for VSMC. TSP1 activity along with that of platelet-derived growth factor (PDGF) regulates VSMC proliferation and migration. However, the manner in which TSP1 and PDGF impact retinal PC function requires further investigation. Here we describe, for ...
Source: Am J Physiol Cell Ph... - February 4, 2009 Category: Cytology Authors: Scheef EA, Sorenson CM, Sheibani N Tags: Am J Physiol Cell Physiol Source Type: journals

Both sides now: multiple interactions of ATP with pannexin-1 hemichannels. Focus on "A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP".Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19179656 [PubMed - in process] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - February 1, 2009 Category: Cytology Authors: Dubyak GR Tags: Am J Physiol Cell Physiol Source Type: journals

Polyamines regulate E-cadherin transcription through c-Myc modulating intestinal epithelial barrier function.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The integrity of the intestinal epithelial barrier depends on intercellular junctions that are highly regulated by numerous extracellular and intracellular factors. E-cadherin is found primarily at the adherens junctions in the intestinal mucosa and mediates strong cell-cell contacts that have a functional role in forming and regulating the epithelial barrier. Polyamines are necessary for E-cadherin expression, but the exact mechanism underlying polyamines remains elusive. The current study was to determine whether polyamines induce E-cadherin expression through the transcription factor c-Myc and if polyamine-regulated...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Liu L, Guo X, Rao JN, Zou T, Xiao L, Yu T, Timmons JA, Turner DJ, Wang JY Tags: Am J Physiol Cell Physiol Source Type: journals

Dynamics of neutrophil extravasation and vascular permeability are uncoupled during aseptic cutaneous wounding.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transport of macromolecules and transmigration of leukocytes across vascular endothelium are regulated by a tight molecular junction, but the mechanisms by which these two inflammatory events are differentially controlled in time and magnitude during aseptic cutaneous wounding remain elusive. A real time fluorescence imaging technique was developed to simultaneously track influx of Alexa-680 labeled albumin and genetically tagged EGFP-neutrophils (PMN) within the wound bed. Vascular permeability increased ~3-fold more rapidly than the rate of PMN influx reaching a maximum at 12 hrs, on the order of ~0.15% per minute ve...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Kim MH, Curry FR, Simon SI Tags: Am J Physiol Cell Physiol Source Type: journals

Vascular endothelial growth factor induces protein kinase D-dependent production of pro-inflammatory cytokines in endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we determined the effect of VEGF on endothelial induction of pro-inflammatory cytokines and investigated the responsible signal pathways. By using a cytokine antibody array that detects the end point protein products released from endothelial cells (ECs), we found that VEGF, via VEGF receptor 2, predominantly induced the production of pro-inflammatory cytokine interleukin (IL)-6 and CXC chemokines IL-8 and growth-related oncogene-alpha (GROalpha) in ECs but not in leukocytes among 36 cytokines in the array. The production of these inflammatory cytokines by VEGF was much stronger than the induction of cell ad...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Hao Q, Wang L, Tang H Tags: Am J Physiol Cell Physiol Source Type: journals

Counter-Regulation of Clathrin-mediated Endocytosis by the Actin and Microtubular Cytoskeleton in Human Neutrophils.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We recently reported that disruption of the actin cytoskeleton enhanced N-formylmethionyl-leucyl-phenylalanine (fMLP)-stimulated granule exocytosis in human neutrophils, but decreased plasma membrane expression of complement receptor 1 (CR1), a marker of secretory vesicles. The present study was initiated to determine if reduced CR1 expression was due to fMLP-stimulated endocytosis, to determine the mechanism of this endocytosis, and to examine its impact on neutrophil functional responses. Stimulation of neutrophils with fMLP or ionomycin in the presence of latrunculin A resulted in uptake of AlexaFluor 488-labeled al...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Uriarte SM, Jog NR, Luerman GC, Bhimani S, Ward RA, McLeish KR Tags: Am J Physiol Cell Physiol Source Type: journals

Ca2+ pathway involved in the refilling of store sites in rat adrenal medullary cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been suggested that store-operated Ca(2+) entry (SOC) facilitates catecholamine secretion and synthesis in bovine adrenal medullary (AM) cells. However, there has been no experimental result clearly showing that cation channel activity is enhanced by store Ca(2+) depletion. Thus, the present experiments were undertaken to address the issue of whether or not rat AM cells have SOC channels. Inhibition of the sarco/endoplasmic reticulum Ca(2+) (SERCA) pump resulted in a sustained increase in [Ca(2+)]i in rat AM cells. This increase was completely suppressed by 2 mM Ni(2+), but not by 100 microM D600. A bath applica...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Matsuoka H, Harada K, Ikeda T, Uetsuki K, Sata T, Warashina A, Inoue M Tags: Am J Physiol Cell Physiol Source Type: journals

The Role of Cell Cholesterol and the Cytoskeleton in the Interaction between IK1 and Maxi-K Channels.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently we demonstrated a novel interaction between large conductance (maxi-K or KCa1.1) and intermediate conductance (IK1 or KCa3.1) Ca(2+)-activated K channels: activation of IK1 channels causes the inhibition of maxi-K activity. Here we show that the interaction between these two channels can be regulated by the membrane cholesterol level in parotid acinar cells. Depletion of cholesterol using methyl-beta-cyclodextrin weakened, while cholesterol enrichment increased, the ability of IK1 activation to inhibit maxi-K channels. Cholesterol's stereoisomer, epicholesterol, was unable to substitute for cholesterol in the ...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Romanenko VG, Roser K, Melvin JE, Begenisich T Tags: Am J Physiol Cell Physiol Source Type: journals

Green tea (-)-epigallocatechin gallate inhibits insulin stimulation of 3T3-L1 preadipocyte mitogenesis via the 67-kilodalton laminin receptor pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the pathways involved in EGCG modulation of insulin-stimulated mitogenesis in 3T3-L1 preadipocytes. EGCG inhibited insulin stimulation of preadipocyte proliferation in a dose- and time-dependent manner. EGCG also suppressed insulin-stimulated phosphorylation of the insulin receptor-beta (IRbeta), insulin receptor substrates 1 and 2 (IRS1 and IRS2), and mitogen-activated protein kinase pathway proteins, RAF1, MEK1/2, and ERK1/2, but not JNK. Furthermore, EGCG inhibited the association of IR with the IRS1 and IRS2 proteins, but not with the IRS4 protein. These data suggest that EGCG selectively affect...
Source: Am J Physiol Cell Ph... - January 28, 2009 Category: Cytology Authors: Ku HC, Chang HH, Liu HC, Hsiao CH, Lee MJ, Hu YJ, Hung PF, Liu CW, Kao YH Tags: Am J Physiol Cell Physiol Source Type: journals

Renal NHE3 and NaPi2 partition into distinct membrane domains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, NHE3 and NaPi2, while both localized to apical microvilli, are segregated into domains: NHE3 to lipid rafts and NaPi2 to non-rafts. These domain properties may play a role in the distinct trafficking patterns observed during elevated pressures: NHE3 remains in rafts and settles to the base of the microvilli while NaPi2 is freely endocytosed. Key words: subcellular fractionation, hypertension, lipid raft, kidney, proximal tubule. PMID: 19158399 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Riquier AD, Lee DH, McDonough AA Tags: Am J Physiol Cell Physiol Source Type: journals

Insights into the residence in lipid rafts of adenylyl cyclase ac8 and its regulation by capacitative calcium entry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
INSIGHTS INTO THE RESIDENCE IN LIPID RAFTS OF ADENYLYL CYCLASE AC8 AND ITS REGULATION BY CAPACITATIVE CALCIUM ENTRY. Am J Physiol Cell Physiol. 2009 Jan 21; Authors: Pagano M, Clynes MJ, Masada N, Ciruela A, Ayling LJ, Wachten S, Cooper DM Adenylyl cyclases (ACs) are a family of critically important signalling molecules that are regulated by multiple pathways. Adenylyl cyclase 8 (AC8) is a Ca(2+) stimulated isoform that displays a selective regulation by capacitative Ca(2+) entry (CCE), the process whereby the entry of Ca(2+) into cells is triggered by the emptying of intracellular stores. This selectivity was ...
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Pagano M, Clynes MJ, Masada N, Ciruela A, Ayling LJ, Wachten S, Cooper DM Tags: Am J Physiol Cell Physiol Source Type: journals

A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
When skeletal muscle is stretched or injured, myogenic satellite cells are activated to enter the cell cycle. This process depends on nitric oxide (NO) production by NO synthase (NOS), matrix metalloproteinase activation, release of hepatocyte growth factor (HGF) from the extracellular matrix, and presentation of HGF to the c-met receptor as demonstrated by a primary culture and in vivo assays. We now add evidence that calcium-calmodulin is involved in the satellite cell activation cascade in vitro. Conditioned medium from cultures which were treated with a calcium ionophore (A23187, ionomycin) for 2 hr activated cultu...
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE Tags: Am J Physiol Cell Physiol Source Type: journals

Gs{alpha} deficiency in skeletal muscle leads to reduced muscle mass, fiber-type switching, and glucose intolerance without insulin resistance or deficiency.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ubiquitously expressed G protein alpha-subunit Gsalpha is required for receptor-stimulated intracellular cAMP responses, and is an important regulator of energy and glucose metabolism. We have generated skeletal muscle-specific GsalphaKO mice (MGsKO) by mating Gsalpha-floxed mice with muscle creatine kinase-cre transgenic mice. MGsKO mice had normal body weight and composition, and their serum glucose, insulin, free fatty acid and triglyceride levels were similar to that of controls. However, MGsKO mice were glucose intolerant despite the fact that insulin sensitivity and glucose-stimulated insulin secretion were n...
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Chen M, Feng HZ, Gupta D, Kelleher J, Dickerson KE, Wang J, Hunt D, Jou W, Gavrilova O, Jin JP, Weinstein LS Tags: Am J Physiol Cell Physiol Source Type: journals

L-Thyroxine vs. 3,5,3'-Triiodo-L-Thyronine and Cell Proliferation: Activation of Mitogen-Activated Protein Kinase and Phosphatidylinositol 3-Kinase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
3,5,3'-Triiodo-L-thyronine (T3), but not L-thyroxine (T4), activated phosphatidyl-inositol 3-kinase (PI 3-K) by a receptor on alphavbeta3 integrin in human glioblastoma U87MG cells. We confirmed that both T3 and T4 activate ERK1/2, but activated ERK1/2 did not contribute to T3-induced PI 3-K activation and an inhibitor of PI 3-K, LY294002, did not block activation of ERK1/2 by physiological concentrations of T3 and T4. Thus, PI 3-K and ERK1/2 are parallel signal transduction pathways in T3-treated U87MG cells. T3 and T4 both caused proliferation of U87MG cells. An ERK1/2 inhibitor, PD98059, blocked the proliferative ac...
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Lin HY, Sun M, Tang HY, Lin C, Luidens MK, Mousa SA, Incerpi S, Drusano GL, Davis FB, Davis PJ Tags: Am J Physiol Cell Physiol Source Type: journals

Phospholemman regulates cardiac Na+/Ca2+ exchanger by interacting with the exchanger's proximal linker domain.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we used intact Na(+)/Ca(2+) exchanger with various deletions in the intracellular loop to map out the interaction sites with PLM. We first demonstrated by Western blotting and confocal immunofluorescence microscopy that wild-type (WT) NCX1 and its deletion mutants were expressed in transfected HEK293 cells. Co-transfection with PLM and NCX1 (or its deletion mutants) in HEK293 cells did not decrease NCX1 (or its deletion mutants) expression. Co-expression of PLM with wild-type NCX1 inhibited NCX1 current (INaCa). Deletion of residues 240-679, 265-373, 250-300 or 300-373 from WT NCX1 resulted in loss of inhibi...
Source: Am J Physiol Cell Ph... - January 21, 2009 Category: Cytology Authors: Zhang XQ, Wang J, Carl LL, Song J, Ahlers BA, Cheung JY Tags: Am J Physiol Cell Physiol Source Type: journals

General redox environment and carotid body chemoreceptor function.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
GENERAL REDOX ENVIRONMENT AND CAROTID BODY CHEMORECEPTOR FUNCTION. Am J Physiol Cell Physiol. 2009 Jan 14; Authors: Agapito MT, Sanz-Alfayate G, Gomez-Niño A, Gonzalez C, Obeso A Carotid body (CB) chemoreceptor cells detect physiological levels of hypoxia and generate a hyperventilation, homeostatic in nature, aimed to minimize the deleterious effects of hypoxia. Intimate mechanisms involved in oxygen-sensing in chemoreceptor cells remain largely unknown, but reactive oxygen species (ROS) had been proposed as mediators of this process. We have determined glutathione levels and calculated glutathione redox ...
Source: Am J Physiol Cell Ph... - January 14, 2009 Category: Cytology Authors: Agapito MT, Sanz-Alfayate G, Gomez-Niño A, Gonzalez C, Obeso A Tags: Am J Physiol Cell Physiol Source Type: journals

Effect of pH on L- and D-methionine uptake across the apical membrane of Caco-2 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, Met transport across the apical membrane of Caco-2 cells is increased by low extracellular pH as the result of the stimulation of two transport systems functionally identified with systems L and B(0,+) for L-Met and with system B(0,+) for D-Met. Key words: intestine, amino acid transport, H+-gradient. PMID: 19144861 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - January 14, 2009 Category: Cytology Authors: Martin-Venegas R, Rodriguez-Lagunas MJ, Mercier Y, Geraert PA, Ferrer R Tags: Am J Physiol Cell Physiol Source Type: journals

Regulation of Epithelial Sodium Channel (ENaC)-Mediated Sodium Transport by Glucocorticoids in Reissner's Membrane Epithelium.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Reissner's membrane epithelium forms much of the barrier that produces and sustains the large ionic differences between cochlear endolymph and perilymph. We have reported that Reissner's membrane contributes to normal cochlear function by absorbing Na(+) from endolymph via amiloride-sensitive channels in gerbil inner ear. In the present study, we sought in mouse Reissner's membrane 1) to identify candidate genes involved in the Na(+) transport pathway, 2) to determine whether their level of expression was regulated by the synthetic glucocorticoid dexamethasone and 3) to obtain functional evidence for the physiologic im...
Source: Am J Physiol Cell Ph... - January 14, 2009 Category: Cytology Authors: Kim SH, Kim KX, Raveendran NN, Wu T, Pondugula SR, Marcus DC Tags: Am J Physiol Cell Physiol Source Type: journals

Both hypoxia and work are required to enhance expression of myoglobin in skeletal muscle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Not applicable Key words: hypoxia, myoglobin, gene expression. PMID: 19144863 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - January 14, 2009 Category: Cytology Authors: Wittenberg BA Tags: Am J Physiol Cell Physiol Source Type: journals

Am J Physiol Cell Physiol; +40 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
40 new PubMed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These PubMed results were generated on 2009/01/08PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - January 8, 2009 Category: Cytology Tags: Report Source Type: journals

Dissecting the Functions of Protein-Protein Interactions: Caveolin as a Promiscuous Partner.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
na. PMID: 19118160 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - December 31, 2008 Category: Cytology Authors: Hardin CD, Vallejo J Tags: Am J Physiol Cell Physiol Source Type: journals

Podocalyxin-like protein is an E-/L-selectin ligand on colon carcinoma cells: Comparative biochemical properties of selectin ligands in host and tumor cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Selectins facilitate metastasis and tumor cell arrest in the microvasculature by mediating binding of selectin-expressing host cells to ligands on tumor cells. We recently identified CD44 variant isoforms as functional P-, but not E-/L-, selectin ligands on colon carcinoma cells. Furthermore, a ~180-kDa sialofucosylated glycoprotein(s) mediated selectin binding in CD44-knockdown cells. Using immunoaffinity chromatography and tandem mass spectrometry, we identify podocalyxin-like protein (PCLP) as an alternative selectin ligand. Blot rolling and cell-free flow-based adhesion assays disclose that PCLP on LS174T colon car...
Source: Am J Physiol Cell Ph... - December 31, 2008 Category: Cytology Authors: Thomas SN, Schnaar RL, Konstantopoulos K Tags: Am J Physiol Cell Physiol Source Type: journals

Nox4 NADPH oxidase mediates oxidative stress and apoptosis caused by TNF{alpha} in cerebral vascular endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Inflammatory brain disease may damage cerebral vascular endothelium leading to cerebral blood flow dysregulation. The pro-inflammatory cytokine TNFalpha causes oxidative stress and apoptosis in cerebral microvascular endothelial cells (CMVEC) from newborn pigs. We investigated contribution of major cellular sources of reactive oxygen species to endothelial inflammatory response. NO synthase and xanthine oxidase inhibitors (L-NNA and allopurinol) had no effect, while mitochondrial electron transport inhibitors (CCCP, TTFA and rotenone) attenuated TNFalpha-induced superoxide (O2(.-)) and apoptosis. NADPH oxidase inhibito...
Source: Am J Physiol Cell Ph... - December 31, 2008 Category: Cytology Authors: Basuroy S, Bhattacharya S, Leffler CW, Parfenova H Tags: Am J Physiol Cell Physiol Source Type: journals

VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Accumulating evidence points to a significant role of vasodilator-stimulated phosphoprotein (VASP) in the maintenance of endothelial barrier functions. We have recently shown that impaired barrier functions in VASP-deficient microvascular myocardial endothelial cells (MyEnd VASP -/-) correlated with decreased Rac 1 activity. To further test the hypothesis that VASP is involved in regulation of Rac 1 activity, we studied cAMP-dependent Rac 1 activation. Both, inhibition of Rac 1 activation by NSC-23766 as well as of protein kinase A (PKA) by PKI completely blunted the efficacy of forskolin/rolipram (F/R)-mediated cAMP i...
Source: Am J Physiol Cell Ph... - December 31, 2008 Category: Cytology Authors: Schlegel N, Waschke J Tags: Am J Physiol Cell Physiol Source Type: journals

Nardilysin convertase regulates the function of the maxi-k channel isoform mk44 in human myometrium.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
NARDILYSIN CONVERTASE REGULATES THE FUNCTION OF THE MAXI-K CHANNEL ISOFORM MK44 IN HUMAN MYOMETRIUM. Am J Physiol Cell Physiol. 2008 Dec 31; Authors: Korovkina VP, Stamnes SJ, Brainard AM, England SK In smooth muscle, the large-conductance calcium and voltage sensitive potassium channel (maxi-K) generate isoforms with disparate responses to contractile stimuli. We have previously shown that the human myometrium expresses high levels of the maxi-K channel isoform mK44. The studies presented here demonstrate that nardilysin convertase, a zinc-dependent metalloprotease of the insulinase family, regulates the plasm...
Source: Am J Physiol Cell Ph... - December 31, 2008 Category: Cytology Authors: Korovkina VP, Stamnes SJ, Brainard AM, England SK Tags: Am J Physiol Cell Physiol Source Type: journals

Map kinase phosphatase 1: a link between cell signalling and histone phosphorylation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
MAP KINASE PHOSPHATASE 1: A LINK BETWEEN CELL SIGNALLING AND HISTONE PHOSPHORYLATION. Am J Physiol Cell Physiol. 2008 Dec 24; Authors: Pagès G The DNA of eukaryotic cells is highly folded and compacted into a dynamic polymer termed chromatin that can be propagated during mitosis. Chromatin contains the entire genome and associated proteins called histones that are subjected to different post-translational modifications, generally in the amino-terminal domain. Distinct modifications including acetylation, methylation, ubiquitination, ADP-ribosylation, glycosylation and phosphorylation dictate dynamic transi...
Source: Am J Physiol Cell Ph... - December 24, 2008 Category: Cytology Authors: Pagès G Tags: Am J Physiol Cell Physiol Source Type: journals

Calpain-1 is Required for Hydrogen Peroxide Induced Myotube Atrophy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent reports suggest numerous roles for cysteine proteases in the progression of skeletal muscle atrophy due to disuse or disease. Nonetheless, a specific requirement for these proteases in the progression of skeletal muscle atrophy has not been demonstrated. Therefore, this investigation determined whether calpains or caspase-3 are required for oxidant induced C2C12 myotube atrophy. We demonstrate that exposure to hydrogen peroxide (25microM H2O2) induces myotube oxidative damage and atrophy, with no evidence of cell death. Twenty-four hours of exposure to H2O2 significantly reduced both myotube diameter and the abu...
Source: Am J Physiol Cell Ph... - December 24, 2008 Category: Cytology Authors: McClung JM, Judge A, Talbert EE, Powers SK Tags: Am J Physiol Cell Physiol Source Type: journals

Tumor Necrosis Factor Alpha Down-Regulates Intestinal NHE8 Expression by Reducing Basal Promoter Activity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our studies suggest that TNF-alpha decreases NHE8 expression in inflammation induced by TNBS and LPS, which may contribute to the diarrhea associated with inflammation. Key words: TNBS colitis, TNF-, NHE8, intestine. PMID: 19109523 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - December 24, 2008 Category: Cytology Authors: Xu H, Chen H, Dong J, Li J, Chen R, Uno JK, Ghishan FK Tags: Am J Physiol Cell Physiol Source Type: journals

Hyperosmotic stress induces Rho/Rho kinase/LIM kinase-mediated cofilin phosphorylation in tubular cells: key role in the osmotically triggered F-actin response.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hyperosmotic stress induces cytoskeleton reorganization and a net increase in cellular F-actin, but the underlying mechanisms are incompletely understood. While de novo F-actin polymerization likely contributes to the actin response, the role of F-actin severing is unknown. To address this problem we investigated whether hyperosmolarity regulates cofilin, a key actin-severing protein, whose activity is inhibited by phosphorylation. Since the small GTPases Rho and Rac are sensitive to cell volume changes, and can regulate cofilin phosphorylation, we also asked if they might link osmostress to cofilin. Here we show that ...
Source: Am J Physiol Cell Ph... - December 24, 2008 Category: Cytology Authors: Thirone AC, Speight P, Zulys M, Rotstein OD, Szaszi K, Pedersen SF, Kapus A Tags: Am J Physiol Cell Physiol Source Type: journals

Molecular Beacons Can Assess Changes in Expression and 3' Polyadenylation of Human eNOS mRNA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The endothelium plays an essential role in maintaining vascular homeostasis, and it fulfills this role by modulating intracellular signaling and gene expression in response to chemical and mechanical stimuli. Assessing changes in endothelial gene expression is essential to understanding how physiologic and pathophysiologic processes modulate vascular homeostasis. Here we describe the use of molecular beacons to rapidly and quantitatively assess expression and 3' polyadenylation of a gene that is important for vascular homeostasis, endothelial nitric oxide synthase (eNOS). Single and dual-FRET molecular beacon hybridiza...
Source: Am J Physiol Cell Ph... - December 24, 2008 Category: Cytology Authors: Jones R, Baker MB, Weber M, Harrison DG, Bao G, Searles CD Tags: Am J Physiol Cell Physiol Source Type: journals