Hypoxic perfusion of pulmonary arterial vasa vasorum increases pulmonary arterial pressure
In conclusion, the results of the current study prove that hypoxic perfusion of the vasa vasorum of the PA directly increases PAP in an ex situ lung perfusion setup suggesting that PA vasa vasorum function and wall ischemia may contribute to the development of PH.PMID:38651234 | DOI:10.1152/ajplung.00346.2023 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Emma L Heise Jawad Salman Karolin S Webs Klaus H öffler Christina Brandenberger Dietmar B öthig Christian M ühlfeld Axel Haverich Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
Aberrant Long-Chain Fatty Acid Metabolism Associated with Evolving Systemic Sclerosis-Associated Pulmonary Arterial Hypertension
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00057.2024. Online ahead of print.ABSTRACTWe sought to investigate differential metabolism in patients with systemic sclerosis (SSc) who develop pulmonary arterial hypertension (PAH) versus those who do not, as a method of identifying potential disease biomarkers. In a nested case-control design, serum metabolites were assayed in SSc subjects who developed right heart catheterization-confirmed PAH (n=22) while under surveillance in a longitudinal cohort from Johns Hopkins, then compared to metabolites assayed in matched SSc patients who did not develop P...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Julie C Coursen Tijana Tuhy Mario Naranjo Adrianne Woods Laura K Hummers Ami A Shah Karthik Suresh Scott H Visovatti Stephen C Mathai Paul M Hassoun Rachel L Damico Catherine E Simpson Source Type: research
A mouse model of progressive lung fibrosis with cutaneous involvement induced by a combination of oropharyngeal and osmotic minipump bleomycin delivery
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00408.2023. Online ahead of print.ABSTRACTSystemic sclerosis (SSc) with interstitial lung disease (SSc-ILD) lacks curative pharmacological treatments, thus necessitating effective animal models for candidate drug discovery. Existing Bleomycin (BLM)-induced SSc-ILD mouse models feature spatially limited pulmonary fibrosis, spontaneously resolving after 28 days. Here, we present an alternative BLM administration approach in female C57BL/6 mice, combining oropharyngeal aspiration (OA) and subcutaneous mini-pump delivery (pump) of BLM to induce a sustained a...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Andrea Grandi Erica Ferrini Matteo Zoboli Davide Buseghin Francesca Pennati Zahra Khalajzeyqami Roberta Ciccimarra Gino Villetti Franco Fabio Stellari Source Type: research
Progesterone amplifies allergic inflammation and airway pathology in association with higher lung ILC2 responses
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00207.2023. Online ahead of print.ABSTRACTPerimenstrual worsening of asthma occurs in up to 40% of asthmatic women, leading to increased acute exacerbations requiring clinical care. The role of sex hormones during these times remains unclear. In the current study, we used a translational approach to determine whether progesterone exacerbates allergic inflammation in the traditional OVA model in BALB/c mice. Simultaneously, we used PBMC from healthy human donors to assess the effects of progesterone on circulating ILC2. Briefly, lungs of ovarectomized (OV...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Shubhanshi Trivedi Cassandra E Deering-Rice Samuel E Aamodt Thomas P Huecksteadt Elizabeth J Myers Karl A Sanders Robert Paine Kristi J Warren Source Type: research
Hypoxic perfusion of pulmonary arterial vasa vasorum increases pulmonary arterial pressure
In conclusion, the results of the current study prove that hypoxic perfusion of the vasa vasorum of the PA directly increases PAP in an ex situ lung perfusion setup suggesting that PA vasa vasorum function and wall ischemia may contribute to the development of PH.PMID:38651234 | DOI:10.1152/ajplung.00346.2023 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Emma L Heise Jawad Salman Karolin S Webs Klaus H öffler Christina Brandenberger Dietmar B öthig Christian M ühlfeld Axel Haverich Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
Aberrant Long-Chain Fatty Acid Metabolism Associated with Evolving Systemic Sclerosis-Associated Pulmonary Arterial Hypertension
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00057.2024. Online ahead of print.ABSTRACTWe sought to investigate differential metabolism in patients with systemic sclerosis (SSc) who develop pulmonary arterial hypertension (PAH) versus those who do not, as a method of identifying potential disease biomarkers. In a nested case-control design, serum metabolites were assayed in SSc subjects who developed right heart catheterization-confirmed PAH (n=22) while under surveillance in a longitudinal cohort from Johns Hopkins, then compared to metabolites assayed in matched SSc patients who did not develop P...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Julie C Coursen Tijana Tuhy Mario Naranjo Adrianne Woods Laura K Hummers Ami A Shah Karthik Suresh Scott H Visovatti Stephen C Mathai Paul M Hassoun Rachel L Damico Catherine E Simpson Source Type: research
A mouse model of progressive lung fibrosis with cutaneous involvement induced by a combination of oropharyngeal and osmotic minipump bleomycin delivery
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00408.2023. Online ahead of print.ABSTRACTSystemic sclerosis (SSc) with interstitial lung disease (SSc-ILD) lacks curative pharmacological treatments, thus necessitating effective animal models for candidate drug discovery. Existing Bleomycin (BLM)-induced SSc-ILD mouse models feature spatially limited pulmonary fibrosis, spontaneously resolving after 28 days. Here, we present an alternative BLM administration approach in female C57BL/6 mice, combining oropharyngeal aspiration (OA) and subcutaneous mini-pump delivery (pump) of BLM to induce a sustained a...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Andrea Grandi Erica Ferrini Matteo Zoboli Davide Buseghin Francesca Pennati Zahra Khalajzeyqami Roberta Ciccimarra Gino Villetti Franco Fabio Stellari Source Type: research
Progesterone amplifies allergic inflammation and airway pathology in association with higher lung ILC2 responses
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00207.2023. Online ahead of print.ABSTRACTPerimenstrual worsening of asthma occurs in up to 40% of asthmatic women, leading to increased acute exacerbations requiring clinical care. The role of sex hormones during these times remains unclear. In the current study, we used a translational approach to determine whether progesterone exacerbates allergic inflammation in the traditional OVA model in BALB/c mice. Simultaneously, we used PBMC from healthy human donors to assess the effects of progesterone on circulating ILC2. Briefly, lungs of ovarectomized (OV...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Shubhanshi Trivedi Cassandra E Deering-Rice Samuel E Aamodt Thomas P Huecksteadt Elizabeth J Myers Karl A Sanders Robert Paine Kristi J Warren Source Type: research
Revisiting airway epithelial dysfunction and mechanisms in chronic obstructive pulmonary disease: Role of mitochondrial damage
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 16. doi: 10.1152/ajplung.00362.2023. Online ahead of print.ABSTRACTChronic exposure to environmental hazards causes airway epithelial dysfunction, primarily impaired physical barriers, immune dysfunction, and repair or regeneration. Impairment of airway epithelial function subsequently leads to exaggerated airway inflammation and remodeling, the main features of chronic obstructive pulmonary disease (COPD). Mitochondrial damage has been identified as one of the mechanisms of airway abnormalities in COPD, which is closely related to airway inflammation and airflow limitation. In ...
Source: Am J Physiol Lung Ce... - April 16, 2024 Category: Respiratory Medicine Authors: Qinglan He Peijun Li Lihua Han Chen Yang Meiling Jiang Yingqi Wang Xiaoyu Han Yuanyuan Cao Xiaodan Liu Weibing Wu Source Type: research
Revisiting airway epithelial dysfunction and mechanisms in chronic obstructive pulmonary disease: Role of mitochondrial damage
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 16. doi: 10.1152/ajplung.00362.2023. Online ahead of print.ABSTRACTChronic exposure to environmental hazards causes airway epithelial dysfunction, primarily impaired physical barriers, immune dysfunction, and repair or regeneration. Impairment of airway epithelial function subsequently leads to exaggerated airway inflammation and remodeling, the main features of chronic obstructive pulmonary disease (COPD). Mitochondrial damage has been identified as one of the mechanisms of airway abnormalities in COPD, which is closely related to airway inflammation and airflow limitation. In ...
Source: Am J Physiol Lung Ce... - April 16, 2024 Category: Respiratory Medicine Authors: Qinglan He Peijun Li Lihua Han Chen Yang Meiling Jiang Yingqi Wang Xiaoyu Han Yuanyuan Cao Xiaodan Liu Weibing Wu Source Type: research
Revisiting airway epithelial dysfunction and mechanisms in chronic obstructive pulmonary disease: Role of mitochondrial damage
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 16. doi: 10.1152/ajplung.00362.2023. Online ahead of print.ABSTRACTChronic exposure to environmental hazards causes airway epithelial dysfunction, primarily impaired physical barriers, immune dysfunction, and repair or regeneration. Impairment of airway epithelial function subsequently leads to exaggerated airway inflammation and remodeling, the main features of chronic obstructive pulmonary disease (COPD). Mitochondrial damage has been identified as one of the mechanisms of airway abnormalities in COPD, which is closely related to airway inflammation and airflow limitation. In ...
Source: Am J Physiol Lung Ce... - April 16, 2024 Category: Respiratory Medicine Authors: Qinglan He Peijun Li Lihua Han Chen Yang Meiling Jiang Yingqi Wang Xiaoyu Han Yuanyuan Cao Xiaodan Liu Weibing Wu Source Type: research
Natural antibodies targeting LPS in pleural effusions of various etiologies
CONCLUSIONS: Our results highlight a potential role of anti-LPS NAbs in the pleural cavity that should be further explored.PMID:38591123 | DOI:10.1152/ajplung.00377.2023 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - April 9, 2024 Category: Respiratory Medicine Authors: Ioannis Sarrigeorgiou Erasmia Rouka Ourania S Kotsiou Garyfallia Perlepe Efrosini S Gerovasileiou Konstantinos I Gourgoulianis Peggy Lymberi Sotirios G Zarogiannis Source Type: research
Chronic intermittent hypoxia elicits distinct transcriptomic responses among neurons and oligodendrocytes within the brainstem of mice
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 9. doi: 10.1152/ajplung.00320.2023. Online ahead of print.ABSTRACTChronic intermittent hypoxia (CIH) is a prevalent condition characterized by recurrent episodes of oxygen deprivation, linked to respiratory and neurological disorders. Prolonged CIH is known to have adverse effects, including endothelial dysfunction, chronic inflammation, oxidative stress, and impaired neuronal function. These factors can contribute to serious comorbidities, including metabolic disorders and cardiovascular diseases. To investigate the molecular impact of CIH, we examined male C57BL/6J mice expose...
Source: Am J Physiol Lung Ce... - April 9, 2024 Category: Respiratory Medicine Authors: Hemalatha Bhagavan Aguan D Wei Luiz M Oliveira Kimberly A Aldinger Jan-Marino Ramirez Source Type: research
