Uremia Impedes Skeletal Myocyte Myomixer Expression and Fusogenic Activity
In patients with chronic kidney disease (CKD), skeletal muscle mass and function are known to occasionally decline. However, the muscle regeneration and differentiation process in uremia has not been extensively studied. In mice with CKD induced by adenine-containing diet, the tibialis anterior muscle injured using a barium chloride injection method recovered poorly as compared to control mice. In the cultured murine skeletal myocytes, stimulation with indoxyl sulfate (IS), a representative uremic toxin, morphologically jeopardized the differentiation, which was counteracted by L-ascorbic acid (L-AsA) treatment. (Source: A...
Source: American Journal of Pathology - April 17, 2024 Category: Pathology Authors: Takaaki Higashihara, Motoki Odawara, Hiroshi Nishi, Takehito Sugasawa, Yumika Suzuki, Satoshi Kametaka, Reiko Inagi, Masaomi Nangaku Tags: Regular Article Source Type: research
Editorial Board
(Source: American Journal of Pathology)
Source: American Journal of Pathology - April 17, 2024 Category: Pathology Source Type: research
Table of Contents
(Source: American Journal of Pathology)
Source: American Journal of Pathology - April 17, 2024 Category: Pathology Source Type: research
Stromal rigidity stress accelerates pancreatic intraepithelial neoplasia progression and chromosomal instability via nuclear PTK2 localization
Since the mechanotransduction by stromal stiffness stimulates the rupture and repair of the nuclear envelope in pancreatic progenitor cells, accumulated genomic aberrations are under selection in the tumor microenvironment. Analysis of cell growth, micronuclei, and γH2AX foci links to mechanotransduction pressure in vivo during serial orthotopic passages of mouse KrasLSL-G12D/+;Trp53flox/flox;Pdx1-Cre (KPC) cancer cells in the tumor and in migrating through the size-restricted 3μm micropores. To search for pancreatic cancer cell-of-origin, analysis of single -cell datasets revealed that the ECM shapes an alternate route ...
Source: American Journal of Pathology - April 15, 2024 Category: Pathology Authors: Li-Yun Ding, Chia-Jung Chang, Szu-Ying Chen, Kuan-Lin Chen, Yueh-Shan Li, Yun-Chieh Wu, Ting-Yi Hsu, Hsin-Yu Ying, Hsin-Yi Wu, Michael, W. Hughes, Chia-Yih Wang, Chih-Han Chang, Ming-Jer Tang, Woei-Jer Chuang, Yan-Shen Shan, Chia-Jung Chang, Po-Hsien Huan Tags: Regular Article Source Type: research
Differentiation of Type 17 Mucosal-Associated Invariant T Cells in Circulation Contributes to the Severity of Sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RAR-related orphan receptor γt in patients with sepsis. The proportion of MAIT17 was correlated with the severity of sepsis. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 8, 2024 Category: Pathology Authors: Xinying Li, Sicheng Fu, Hao Cheng, Min Ma, Zijian Song, Jun Li, Shuang Wu, Chong Zhang, Xiaoxia Wang, Maoyu Tang, Xuexue Pu, Qiang Ji, Jinquan Liang, Zhibin Zhao, Heinrich K örner, Bin Li, Min Shao, Hua Wang Tags: Regular article Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and ROR γt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 8, 2024 Category: Pathology Authors: Xinying Li, Sicheng Fu, Hao Cheng, Min Ma, Zijian Song, Jun Li, Shuang Wu, Chong Zhang, Xiaoxia Wang, Maoyu Tang, Xuexue Pu, Qiang Ji, Jinquan Liang, Zhibin Zhao, Heinrich K örner, Bin Li, Min Shao, Hua Wang Tags: Regular Article Source Type: research
This Month in AJP
The impact of the functions of serine —a non-essential amino acid—on the pathogenesis of inflammatory bowel diseases (IBD) is unclear. Using human patient samples and mouse models of colitis, Bai and Saha et al (Am J Pathol, AJPA-D-23-00501) studied this impact. Serine synthesis selectively increases in the epithelial cells of colon s from both patients with IBD and mouse models of colitis. Inhibiting serine synthesis impaired epithelial cell proliferation in vivo. Dietary removal of serine also sensitized mice to acute chemically-induced colitis but improved inflammation in chronic colitis models. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 8, 2024 Category: Pathology Tags: This Month in AJP Source Type: research
Transcription Factor E3/Phosphatidylinositol 3-Kinase/Akt/Mammalian Target of Rapamycin Axis in Renal Cell Carcinoma Affects Tumor Microenvironment
This study aimed to determine the relationship between TFE3 and the PI3K/Akt pathway. TFE3 down-regulation was achieved by transient transfection of siRNA and shRNA in UOK146 cells. TFE3 overexpression was induced by transient transfection with pcDNA3.1 encoding the constitutively active form of TFE3. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Chung Su Hwang, Yun Kyung Kang, Ji Yun Kim, So Hyun Shin, Joon Young Park, Ji Sun Song, So Young Kim, Se Jin Jung, Jung Hee Lee, Ju-Young Na, Dong Hoon Shin, Jee Yeon Kim, Sung Woo Park, Hyun Jung Lee Tags: Regular article Source Type: research
Role of HNF4 α-cMyc Interaction in CDE Diet–Induced Liver Injury and Regeneration
This study investigated the role of the HNF4α-cMyc interaction in regulating liver injury and regeneration using the choline-deficient and ethionine-supplemented (CDE) diet model. Wild-type (WT), hepatocyte-specific HNF4α-knockout (KO), cMyc-KO, and HNF4α-cMyc double KO (DKO) mice were fed a CDE diet for 1 week to induce subacute liver injury. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Manasi Kotulkar, Julia Barbee, Diego Paine-Cabrera, Dakota Robarts, Maura O ’Neil, Udayan Apte Tags: Regular article Source Type: research
TFE3/PI3K/Akt/mTOR axis in renal cell carcinoma affects tumor microenvironment
The role of the PI3K/Akt pathway in renal cell carcinoma (RCC) progression, metastasis, and resistance to therapies has not been thoroughly investigated. Transcription factor E3 (TFE3) expression is related to poorer prognosis and tumor microenvironment in patients with RCC. We aimed to determine the relationship between TFE3 and the PI3K/Akt pathway. TFE3 downregulation was achieved by transient transfection of small interfering RNA (siRNA) and short hairpin RNA (shRNA) in UOK146 cells. TFE3 overexpression was induced by transient transfection with pcDNA3.1 encoding the constitutively active form of TFE3. (Source: America...
Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Chung Su Hwang, Yun Kyung Kang, Ji Yun Kim, So Hyun Shin, Joon Young Park, Ji Sun Song, So Young Kim, Se Jin Jung, Jung Hee Lee, Ju-Young Na, Dong Hoon Shin, Jee Yeon Kim, Sung Woo Park, Hyun Jung Lee Tags: Regular Article Source Type: research
Role of HNF4alpha-cMyc Interaction in CDE-diet Induced Liver Injury and Regeneration
This study investigated the role of HNF4α-cMyc interaction in regulating liver injury and regeneration using the cho line-deficient and ethionine-supplemented (CDE) diet model. WT, hepatocyte-specific HNF4α-KO, cMyc-KO, and HNF4α-cMyc double knockout (DKO) mice were fed a CDE diet for one week to induce subacute liver injury. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Manasi Kotulkar, Julia Barbee, Diego Paine-Cabrera, Dakota Robarts, Maura O ’Neil, Udayan Apte Tags: Regular Article Source Type: research
Graph perceiver network for lung tumor and bronchial premalignant lesion stratification from histopathology
Bronchial premalignant lesions (PMLs) precede the development of invasive lung squamous carcinoma (LUSC), posing a significant challenge in distinguishing those likely to advance to LUSC from those that might regress without intervention. In this context, we present a novel computational approach, the Graph Perceiver Network (GRAPE-Net), leveraging hematoxylin and eosin (H&E) stained whole slide images (WSIs) to stratify endobronchial biopsies of PMLs across a spectrum from normal to tumor lung tissues. (Source: American Journal of Pathology)
Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Rushin H. Gindra, Yi Zheng, Emily J. Green, Mary E. Reid, Sarah A. Mazzilli, Daniel T. Merrick, Eric J. Burks, Vijaya B. Kolachalama, Jennifer E. Beane Tags: Regular Article Source Type: research
Title Page
(Source: American Journal of Pathology)
Source: American Journal of Pathology - April 1, 2024 Category: Pathology Source Type: research
Table of Contents
(Source: American Journal of Pathology)
Source: American Journal of Pathology - April 1, 2024 Category: Pathology Source Type: research
Meeting Abstracts
(Source: American Journal of Pathology)
Source: American Journal of Pathology - April 1, 2024 Category: Pathology Source Type: research
