Biosensing-based quality control monitoring of the higher-order structures of therapeutic antibody domains
Anal Chim Acta. 2024 May 15;1303:342439. doi: 10.1016/j.aca.2024.342439. Epub 2024 Feb 29.ABSTRACTAdvanced biopharmaceutical manufacturing requires novel process analytical technologies for the rapid and sensitive assessment of the higher-order structures of therapeutic proteins. However, conventional physicochemical analyses of denatured proteins have limitations in terms of sensitivity, throughput, analytical resolution, and real-time monitoring capacity. Although probe-based sensing can overcome these limitations, typical non-specific probes lack analytical resolution and provide little to no information regarding which...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Hideki Watanabe Naoko Hayashida Megumi Sato Shinya Honda Source Type: research
FeNi-MIL-88B-based electrochemiluminescence immunosensor for ultra-sensitive detection of CD44 protein via dual-quenching strategy
Anal Chim Acta. 2024 May 15;1303:342520. doi: 10.1016/j.aca.2024.342520. Epub 2024 Mar 22.ABSTRACTBACKGROUND: Cluster of Differentiation 44 (CD44) is considered an important biomarker for various cancers, and achieving highly sensitive detection of CD44 is crucial, which plays a significant role in tumor invasion and metastasis, providing essential information for clinical tumor diagnosis. Commonly used methods for analysis include fluorescence spectroscopy (FL), photoelectrochemical analysis (PEC), electrochemical analysis (EC), and commercial ELISA kits. Although these methods offer high sensitivity, they can be relative...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Haiyang Li Li Dai Qiuyu Huang Jianxi Song Lipeng Liu Yan Li Hongmin Ma Qin Wei Source Type: research
A tutorial on potentiometric data processing. Analysis of software for optimization of protonation constants
Anal Chim Acta. 2024 May 15;1303:342476. doi: 10.1016/j.aca.2024.342476. Epub 2024 Mar 17.ABSTRACTDefining the distribution of the chemical species in a multicomponent system is a task of great importance with applications in many fields. To clarify the identity and the abundance of the species that can be formed by the interaction of the components of a solution, it is fundamental to know the formation constants of those species. The determination of equilibrium constants is mainly performed through the analysis of experimental data obtained by different instrumental techniques. Among them, potentiometry is the elective t...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Silvia Berto Salvador Blasco Lorenzo Castellino Aleksandar Cvetkovski Concetta De Stefano Sofia Gama Enrique Garc ía-España Petr Hermann Gabriele Lando Matteo Marafante Michel Meyer Winfried Plass Lauryn Quinodoz Demetrio Milea Source Type: research
Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA
Anal Chim Acta. 2024 May 15;1303:342477. doi: 10.1016/j.aca.2024.342477. Epub 2024 Mar 22.ABSTRACTCRISPR/Cas12a-based nucleic acid assays have been increasingly used for molecular diagnostics. However, most current CRISPR/Cas12a-based RNA assays require the conversion of RNA into DNA by preamplification strategies, which increases the complexity of detection. Here, we found certain chimeric DNA-RNA hybrid single strands could activate the trans-cleavage activity of Cas12a, and then discovered the activating effect of split ssDNA and RNA when they are present simultaneously. As proof of concept, split nucleic acid-activated...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Wen He Xinyu Li Xinmin Li Minghui Guo Mengxuan Zhang Ruiwei Hu Menghan Li Shijia Ding Yurong Yan Source Type: research
Sandwich-type electrochemical aptasensor based on hemin-graphite oxide as a signal label and rGO/MWCNTs/chitosan/carbon quantum dot modified electrode for sensitive detection of Acinetobacter baumannii bacteria
In this study, we present an electrochemical aptasensor based on a signal amplification strategy for the detection of A. baumannii, the high specificity and affinity of the aptamer for the target make it favorable for signal amplification. This allows for a highly sensitive and selective detection of the target. The aptasensor is based on a carbon screen-printed electrode (CSPE) that has been modified with a nanocomposite consisting of multi-walled carbon nanotubes (MWCNTs), reduced graphene oxide (rGO), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS. Additionally, the self-assembled aptamers were immobi...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Rokhsareh Abedi Jahan Bakhsh Raoof Mojtaba Mohseni Ayemeh Bagheri Hashkavayi Source Type: research
Dicationic imidazole ionic liquid stationary phase for preservative detection and its application under mixed mode of HILIC/RPLC/IEC
Anal Chim Acta. 2024 May 15;1303:342504. doi: 10.1016/j.aca.2024.342504. Epub 2024 Mar 23.ABSTRACTBACKGROUND: Food safety has always been a great concern, and the detection of additives is vital to ensuring food safety. Therefore, there is a necessity to develop a method that can quickly and efficiently separate and detect additives in food. High performance liquid chromatography is widely used in the analysis and testing of food additives. Ionic liquids have attracted wide attention in the preparation of high performance liquid chromatography stationary phases owing to their high stability, low vapor pressure and adjustab...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Hanqi Yang Jingdong Peng Huanjun Peng Hanlin Zeng Jiayu Yu Jiajia Wu Xiang Wang Source Type: research
SCREENES: Enhancing non-uniform sampling reconstruction for symmetrical NMR spectroscopy
Anal Chim Acta. 2024 May 15;1303:342510. doi: 10.1016/j.aca.2024.342510. Epub 2024 Mar 25.ABSTRACTBACKGROUND: Symmetrical NMR spectroscopy, such as Total Correlation Spectroscopy (TOCSY) and other homonuclear spectroscopy, displays symmetry in chemical shift but are generally not symmetrical in terms of intensity, which constitutes a pivotal branch of multidimensional NMR spectroscopy and offers a robust tool for elucidating the structures and dynamics of complex samples, particularly in the context of biological macromolecules. Non-Uniform Sampling (NUS) stands as a critical technique for accelerating multidimensional NMR...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Ze Fang Bo Chen Chengda Huang Yifei Yuan Yao Luo Liubin Wu Yida Chen Yuqing Huang Yu Yang Enping Lin Zhong Chen Source Type: research
Determination of fatty acid uptake and desaturase activity in mammalian cells by NMR-based stable isotope tracing
Anal Chim Acta. 2024 May 15;1303:342511. doi: 10.1016/j.aca.2024.342511. Epub 2024 Mar 21.ABSTRACTBACKGROUND: Mammalian cells both import exogenous fatty acids and synthesize them de novo. Palmitate, the end product of fatty acid synthase (FASN) is a substrate for stearoyl-CoA desaturases (Δ-9 desaturases) that introduce a single double bond into fatty acyl-CoA substrates such as palmitoyl-CoA and stearoyl-CoA. This process is particularly upregulated in lipogenic tissues and cancer cells. Tracer methodology is needed to determine uptake versus de novo synthesis of lipids and subsequent chain elongation and desaturation. ...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Penghui Lin James Sledziona Kubra B Akkaya-Colak Maria M Mihaylova Andrew N Lane Source Type: research
Novel CRISPR/SpRY system for rapid detection of CRISPR/Cas-mediated gene editing in rice
This study aimed to develop a novel and efficient method, termed the CRISPR/SpRY detection platform, for the rapid screening of CRISPR/Cas9-induced mutants based on CRISPR/SpRY-mediated in vitro cleavage using rice (Oryza sativa L.) samples genetically edited at the TGW locus as an example. We designed the workflow of the CRISPR/SpRY detection platform and conducted a feasibility assessment. Subsequently, we optimized the reaction system of CRISPR/SpRY, and developed a one-pot CRISPR/SpRY assay by integrating recombinase polymerase amplification (RPA). The sensitivity of the method was further verified using recombinant pl...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Zhixun Su Xiaofu Wang Xiaoyun Chen Lin Ding Xiaoqun Zeng Junfeng Xu Cheng Peng Source Type: research
Dual biomarkers-activatable hollow MnO < sub > 2 < /sub > -Based theranostic nanoplatform for efficient breast cancer-specific multisite fluorescence imaging and synergistic therapy
Anal Chim Acta. 2024 May 15;1303:342521. doi: 10.1016/j.aca.2024.342521. Epub 2024 Mar 22.ABSTRACTBACKGROUND: Theranostic nanoplatforms with integrated diagnostic imaging and multiple therapeutic functions play a vital role in precise diagnosis and efficient treatment for breast cancer, but unfortunately, these nanoplatforms are usually stuck in single-site imaging and single mode of treatment, causing unsatisfactory diagnostic and therapeutic efficiency. Herein, a dual biomarkers-activatable facile hollow mesoporous MnO2 (H-MnO2)-based theranostic nanoplatform, DNAzyme@H-MnO2-MUC1 aptamer (DHMM), was constructed for the s...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Juan Ning Guizhen Hu Tian Wu Yijun Zhao Yamin Nie Yanmei Zhou Source Type: research
Electronic waste analysis using laser-induced breakdown spectroscopy (LIBS) and X-ray fluorescence (XRF): Critical evaluation of data fusion for the determination of Al, Cu and Fe
In conclusion, this research represents an important step toward a more sustainable and efficient future for electronic waste recycling, signifying its relevance to global environmental welfare and resource conservation.PMID:38609264 | DOI:10.1016/j.aca.2024.342522 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Dennis S Ferreira Fabiola M V Pereira Alejandro C Olivieri Edenir R Pereira-Filho Source Type: research
Semiconducting polymer dots based l-lactate sensor by enzymatic cascade reaction system
Anal Chim Acta. 2024 May 15;1303:342523. doi: 10.1016/j.aca.2024.342523. Epub 2024 Mar 23.ABSTRACTBACKGROUND: l-lactate detection is important for not only assessing exercise intensity, optimizing training regimens, and identifying the lactate threshold in athletes, but also for diagnosing conditions like L-lactateosis, monitoring tissue hypoxia, and guiding critical care decisions. Moreover, l-lactate has been utilized as a biomarker to represent the state of human health. However, the sensitivity of the present l-lactate detection technique is inadequate.RESULTS: Here, we reported a sensitive ratiometric fluorescent prob...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Shuyi He Weichao Liu Steven Xu Wu Source Type: research
Self-validating photothermal and electrochemical dual-mode sensing based on Hg < sup > 2+ < /sup > etching Ti < sub > 3 < /sub > C < sub > 2 < /sub > MXene
Anal Chim Acta. 2024 May 15;1303:342525. doi: 10.1016/j.aca.2024.342525. Epub 2024 Mar 23.ABSTRACTMercury ions can cause serious damage to the ecological environment, and it is necessary to develop reliable and elegant mercury ion sensors. In this protocol, a label-free photothermal/electrochemical dual-mode strategy for Hg2+ is proposed based on delaminated Ti3C2 MXene nanosheets (DL-Ti3C2 MXene). Hg2+ exists in water in the form of HgCl2, Hg(OH)2, and HgClOH, and the electron-rich elements O and Cl can specifically bind to the positively charged DL-Ti3C2 MXene at the edge, and further oxidation-reduction reaction occurs ...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Yanxin Zhang Jing Li Xiaobing Li Jingchun Lv Qin Xu Hongbo Li Source Type: research
Developing a novel strategy for fabricating matrix film to assess the distribution of potassium perfluorooctanic sulfonate by matrix-assisted laser desorption/ionization mass spectrometry imaging
Anal Chim Acta. 2024 May 15;1303:342528. doi: 10.1016/j.aca.2024.342528. Epub 2024 Mar 25.ABSTRACTMatrix deposition plays a critical role in image quality of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). To improve the ionization efficiency and overcome the limitation of traditional matrix deposition methods in the face of difficult-to-sublimate or difficult-to-dissolve matrix, covalent organic frameworks (COFs) named COF-DhaTab was successfully synthesized and firstly used as matrix film. It was fabricated by imprinting of sieved COF-DhaTab powder on the surface of a double-sided adhes...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Yake Luo Shanshan Ma Jianxun Zhang Qidong Zhang Yanhao Zhang Jian Mao Hang Yuan Gangfeng Ouyang Shusheng Zhang Wuduo Zhao Source Type: research
Target and suspect screening of psychoactive substances in seizures and oral fluid exploiting retention time prediction and LC-MS/MS analysis
Anal Chim Acta. 2024 May 15;1303:342529. doi: 10.1016/j.aca.2024.342529. Epub 2024 Mar 25.ABSTRACTBACKGROUND: Novel psychoactive substances (NPS) are a group of substances, mainly of synthetic origin, characterized by toxicological properties extremely dangerous. The main difficulty in recognizing NPS in seizures and biological samples lies in their dynamic nature, related to the continuous synthesis and introduction on the market of new drugs, often with very similar structures to existing ones. The aim of this study was the creation of a robust and versatile method for the analysis of traditional drugs and NPS in differe...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Gaia Di Francesco Flaminia Vincenti Camilla Montesano Ilenia Bracaglia Martina Croce Sabino Napoletano Antonietta Lombardozzi Manuel Sergi Source Type: research
